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HomeMy WebLinkAboutCPF 2014 Fish Community TemplatesFISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification AVENTS CR SR 1418 108/14/131 BF41 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion HARNETT 7 1 03030004 1 35.4872014-78.90999 18-13-(2) Northern Outer Piedmont Stream Classification Drainage Area (mi) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-IV;HQW 14.2 150 6 0.4 Yes Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 100 0 0 0 Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q., MGD) None I --- I --- Water Quality Parameters Temperature (°C) 23.5 Dissolved Oxygen (mg/L) 7.3 Specific Conductance (pS/cm) 62 pH (s.u.) 5.2 Water Clarity I Turbid -tannic Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 14 4 7 8 5 6 8 5 5 67 Substrate lGravel, cobble, bedrock, and, clay Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 08/14/13 1 2013-50 11 40 Good -Fair 06/10/09 2009-63 17 46 Good 06/06/03 2003-22 15 44 Good -Fair 09/21 /98 98-68 14 48 Good Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Bluehead Chub and White Shiner (n=120 None; 1 of only 2 sites where no nonindigenous (34%) and 105 (30%), respectively). Exotic Species species were collected. Gained -- Eastern Mosquitofish first collection ever and Redfin Pickerel Lost -- Rosyside Dace (n=8), Creek Chubsucker (n=14, young -of -year only), Green Sunfish, Warmouth, Bluegill (n=12), Redear Sunfish, and Largemouth Bass (young -of -year only), Flat Bullhead. Unless otherwise noted, all species gained or lost were represented by 1-3 fish/species. Watershed -- drains northwestern Harnett County; no municipalities or NPDES dischargers within the watershed; headwaters are within the Rolling Coastal Plain, although most of the watershed is in the Northern Outer Piedmont; tributary to the Cape Fear River, site is — 1.2 miles above the creek's confluence with the river and upstream from a waterfall which functions as a natural barrier to upstream fish migration and recolonization except under extremely high flows; site is within Raven Rock State Park. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 51 % forest, 21 % cultivation, 6% developed. and 13% grassland/herbaceous; since 1992 forested land has decreased from 61 % to 51 % and developed land has increased from 1 % to 6%. Habitat -- low flow the day of the sampling even though it was a wet spring and summer with persistently and extremely high flows; habitats appeared similar to what they were during previous cycle; runs, two well -developed riffles, undercuts; cobble bars, and shallow pools; open canopy due to loss of trees from frequent storms. Water Quality -- second lowest specific conductance of any fish community site in 2013, slightly increasing, ranging from 47 pS/cm in 1998 to 62 pS/cm in 2013. 2013 -- number of fish declined by 40% between 2009 and 2013 (583 vs. 351); loss of six species including suckers and sunfish and fewer species with multiple age classes (resulting from extremes in flows) resulted in a decrease in the NCIBI score and rating. 2004-2013 -- only 19 species are known from the site, including 1 species of darter and 2 nonindigenous species, but several species of minnows (e.g., Satinfin Shiner, Swallowtail Shiner, Highfin Shiner, Dusky Shiner, Sandbar Shiner, and Comely Shiner), species of redhorse suckers, and the intolerant Piedmont Darter are absent; dominant species is the Bluehead Chub (38%); due to the natural barrier downstream, an Excellent rating may be unachievable for this regional reference site. Recommendation -- based upon all the data collected, there has been no long-term change in water quality in the Avents Creek watershed; community is likely affected more so by flow extremes than by changes in water quality; continued basinwide assessment of this regional reference site in 2018 to document any potential impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification BEAR CR SR 2187 105/16/131 BF56 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CHATHAM 1 12 03030003 1 35.62558751 -79.298785 17-43-16b Carolina Slate Belt Stream Classification Drainage Area (mi) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C 42.6 1 354 9 0.3 1 No Forested/Wetland Urban Visible Landuse (%) 100 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) Agriculture Other (describe) 0 0 NPDES Number Volume (Qw, MGD) None Water Quality Parameters Temperature (°C) Dissolved Oxygen (mg/L) R6.1 Specific Conductance (pS/cm) pH (s.u.) Water Clarity I Clear -tannic Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 16 10 7 8 4 6 8 5 5 74 Substrate lCobble, boulder, bedrock, gravel, sand Sample Date Sample ID Species Total NCIBI Score NCIBI Rating 05/16/13 2013-27 16 48 Good 06/12/08 2008-60 17 42 Good -Fair 05/15/08 2008-40 17 44 Good -Fair 04/17/08 2008-19 14 46 Good 03/13/08 2008-03 11 34 Fair 06/13/03 2003-25 1 13 1 44 1 Good -Fair 10/29/99 99-75 14 36 Fair 04/07/99 99-03 17 40 Good -Fair 04/23/98 98-21 19 50 Good Most Abundant Species, 2013 lGreen Sunfish (n=49, 19%) Exotic Species lGreen Sunfish (n=49) �G.idne-- Flat Bullhead first collection ever; Yellow Bullhead (n=7), Margined Madtom, and Carolina Darter. Species Change Since Last Cycle Lost -- Whitemouth Shiner, Chain Pickerel, Pumpkinseed, sunfish hybrid, and Largemouth Bass. Unless otherwise noted, all species gained or lost were represented by 1-3 fish/species. uata Watershed --drains southwestern Chatham County; no municipalities in the watershed; two small NPDES dischargers (NC0039331 and NC0039381, Total Qw = 0.017 MGD) located on unnamed tributaries in the headwaters of the creek; large tributary to the Rocky River. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories > 5% are reported) -- 53% forest, 33% cultivation, 5% developed, and grassland/herbaceous 5%; since 1992 forested land has decreased from 67% to 53% and developed land has increased from 1 % to 5%. Habitat -- a Carolina Slate Belt -type stream with angular rocks; shallow cobble riffles with low flow; deep pools at the beginning and end of the reach; Justicia, Podostemum, and Fissidens growing in the riffles; instream habitats are especially flow -dependent; no change in habitats since last cycle. Water Quality -- dischargers may be contributing to the elevated specific conductance which has fluctuated from 91 pS/cm in 1998 to 148 pS/cm in 2008, declined slightly in 2013; dissolved oxygen, recorded early in the morning, only at 62% of saturation. 2013 -- less dominance by Insectivores than in 2008 resulting in a more trophically balanced community, an increase in the NCIBI score, and increase in the rating. 1998-2013 -- 25 species are known from this site including 3 species of darters (including the Carolina Darter, Etheostoma collis , a state Special Concern Species which has been collected six times since 1998), 1 intolerant species, and only 1 nonindigenous species; intolerant Piedmont Darter has not been collected since 2003; dominant species is Highfin Shiner (26%); number of fish collected has been stable since April 2008 (range 225-283); drought recovery studies conducted in 2008 were summarized in BAU Memorandum F-20090122. Recommendation -- based upon all the data collected, no persistent long-term change in water quality in the Bear Creek watershed; except for the two Fair ratings during extremely low flow events, the community ratings have fluctuated between Good -Fair and Good; continued basinwide assessment of this site in 2018 to document any potential impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification BIG ALAMANCE CR SR 3088 104/23/131 BF68 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion GUILFORD 3 03030002 1 35.9988541 -79.642911 16-19-(1) Southern Outer Piedmont Stream Classification Drainage Area (mi) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site W S-IV; NS W 30.5 585 7 0.3 No Forested/Wetland Rural Residential Agriculture Other (describe) Visible Landuse (%) 50 25 25 0 Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q_ MGD) None Water Quality Parameters Temperature (°C) 12.9 Dissolved Oxygen (mg/L) 9.3 Specific Conductance (pS/cm) 116 pH (s.u.) 6.5 Water Clarity I Clear, slightly stained Habitat Assessment Scores (max) Channel Modification (5) 5 Instream Habitat (20) 12 Bottom Substrate (15) 3 Riffle Habitat (16) 5 Pool Variety (10) 4 Erosion (7) 4 Bank Vegetation (7) 6 Light Penetration (10) 8 Left Riparian Score (5) 5 Right Riparian Score (5) 2 Total Habitat Score (100) 54 Sample Date Sample ID Substrate Isand, gravel, boulders along the right bank Species Total NCIBI Score NCIBI Rating 04/23/13 2013-10 17 50 Good 04/16/09 2009-14 20 44 Good -Fair 04/22/03 2003-04 15 42 Good -Fair 10/26/99 99-70 10 36 Fair 04/20/99 99-21 19 48 Good 04/08/98 98-14 20 52 Good Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Swallowtail Shiner and Speckled Killifish Green Sunfish and Redear Sunfish (n=16 and 1, (n=65 (22%) and 61 (21%) respectively) Exotic Species respectively) Gained -- Sandbar Shiner and Margined Madtom. Lost -- Creek Chub, Notchlip Redhorse, Pirate Perch, Warmouth, Largemouth Bass, and Black Crappie. All species gained or lost were represented by 1-3 fish/species. Watershed -- drains southeastern Guilford County with its headwaters dissected by the US 421 corridor; no NPDES dischargers in the watershed; site is — 7 miles upstream from Lake Mackintosh; tributary to the Haw River. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 52% forest, 34% cultivation, and 10% developed; since 1992 forested land has decreased from 63% to 52% and developed land has increased from 4% to 10%. Habitat -- sandy runs, sticks in the current, rip/rap boulders along the right bank at the beginning of the reach; blow -outs along both banks, especially along the left bank at the end of the reach; shallow, sandy stick and gravel riffles and runs; same habitats as in previous cycles. Water Quality -- specific conductance relatively stable, ranging from 91 pS/cm in 2003 to 121 pS/cm in 1999. 2013 -- increase in NCIBI score and rating due to a more balanced trophic structure and more species with multiple age classes (groups) than in 2009 indicating no lingering effects from drought. 1998-2013 -- continued increase in the NCIBI scores and ratings since October 1999 (a severe drought); a diverse community with 29 species known from the site, including 3 species of suckers,3 nonindigenous species, and 2 species of darters, but several species, including the intolerant Piedmont Darter, have not been collected since 1999; dominant species is the Highfin Shiner (23%); community is strongly influenced by flow conditions with recolonization sources limited by the downstream reservoir; stream may go intermittent during sustained droughts. Recommendation -- based upon all the data collected, the community is affected more so by low flow affects that by changes in water quality; no long-term change in water quality; continued basinwide assessment of this site in 2018 to document any potential impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification BOLIN CR off SR 1750 105/10/131 BF14 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion ORANGE 6 03030002 35,926322 79.028269 16-41-1-15-1-(4) Triassic Basins Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-IV;NSW 1 11.5 1 258 8 0.3 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 1 0 100 0 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None I --- I --- Water Quality Parameters Temperature (°C) 16.3 Dissolved Oxygen (mg/L) 8.8 Specific Conductance (pS/cm) 136 pH (s.u.) 6.7 Water Clarity I Slightly turbid Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 4 12 3 12 4 3 6 10 2 2 58 Substrate Sand, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 05/10/13 2013-16 17 40 Good -Fair 10/22/01 2001-92 20 44 Good -Fair 05/18/98 98-38 12 40 Good -Fair Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Speckled Killifish (n=191, 30%) Exotic Species Green Sunfish and Redear Sunfish (n=12 and 2, respectively) Gained -- Creek Chubsucker (n=11) and Redfin Pickerel (n=2) first collections ever. Lost -- Highfin Shiner (n=1), Yellow Bullhead (n=1), Brown Bullhead (n=4), sunfish hybrid (n=1), Black Crappie (n=6), and Yellow Perch (n=1). ed -- drains northern portions of the cities of Chapel Hill and Carrboro in southern Orange County; no NPDES dischargers in the watershed; to Little Creek, New Hope Creek, and ultimately Jordan Reservoir. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 48% forest and 45% developed; since 1992 forested land has decreased from 58% to 48% and developed land has increased from 36% to 45%. Habitat -- gravelly riffles; snags and undercuts; embedded sand and gravel substrate; deep pools absent; entrenched channel; narrow riparian zones; hydrologically flashy due to its developed watershed. Water Quality -- elevated specific conductance from urban stormwater runoff, ranging from 136 pS/cm in 2013 to 210 pS/cm in 2001. 2013 -- most fish (n=633) collected from any site in the basin; moderately high percentage of tolerant fish (42%, Creek Chub, Eastern Mosquitofish, Redbreast Sunfish, and Green Sunfish); very skewed trophic structure (2% Omnivores+Herbivores and 97% Insectivores). 2004-2013 -- very abundant and fairly diverse stream for its size with 22 species known from the site including 2 nonindigenous species, but no intolerant species; dominant species are Bluehead Chub (23%) and Speckled Killifish (19%); site has consistently rated Good -Fair. Recommendation -- based upon all the data collected, there has been no change in water quality in the Bolin Creek watershed; continue monitoring this site in 2018 to document impacts from increasing urbanization of its watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification BRUSH CR SR 1102 105/15/131 BF24 I Excellent County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CHATHAM 9 03030003 35,7093087 79.5400259 17-23a Carolina Slate Belt Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C 119.1 1 507 7 0.3 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 25 0 75 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None I --- I --- Water Quality Parameters Temperature (°C) R6.9 Dissolved Oxygen (mg/L)Specific Conductance (pS/cm)pH (s.u.) Water Clarity I Clear, easily silted Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 18 12 16 8 7 7 9 4 5 91 Substrate Cobble, boulder, bedrock, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Rating 05/15/13 2013-24 18 56 Excellent 06/08/09 2009-56 18 50 Good 06/26/03 2003-36 16 52 Good Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Highfin Shiner (n=227, 45%) Exotic Species Green Sunfish and Redlip Shiner (n=24 and 1, respectively) Gained -- Creek Chub first collection ever; Chain Pickerel and Warmouth. Lost -- Rosyside Dace, Eastern Mosquitofish (n=6), and Redear Sunfish. Unless otherwise noted, all species gained or lost were represented by 1 or 2 fish/species. Watershed -- drains the extreme western portion of Chatham and extreme eastern portion of Randolph counties; no municipalities within the watershed; one small permitted discharger (NC0038300, Qw = 0.01 MGD) located - 3 miles upstream; large tributary to the Deep River, site is in the creek's upper reaches. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 50% forest, 36% cultivation, 6% developed, and 6% grassland/herbaceous; since 1992 forested land has decreased from 67% to 50% and developed land has increased from 1 % to 6%. Habitat -- typical high -quality Carolina Slate Belt -type stream with angular rocks covered by slick periphyton; snags; shallow boulder and side pools; shallow, short and frequent plunge -type riffles; Podostemum and Fissidens abundant; habitats have not changed since 2003; 2nd highest total habitat score of any fish community site in the basin; low flow. Water Quality -- specific conductance stable, ranging from 96 pS/cm in 2009 to 107 pS/cm in 2013. 2013 -- more balanced trophic structure (due to an abundance of the pool- and run -dwelling insectivorous Highfin Shiner and fewer omnivorous Bluehead Chub) than in the previous cycle resulted in the 6 point increase in score and the improvement in rating from Good to Excellent; only metrics not scoring the maximum score (5) were the Number of Species of Suckers and the Percentage of Piscivores. 2003-2013 -- 22 species known from the site, including 2 species of darters, 1 intolerant species, and 3 nonindigenous species; dominant species is the Bluehead Chub (24%) and Highfin Shiner (20%). Recommendation -- based upon all the data collected, the fish community data suggest an improvement in water quality in the Brush Creek watershed; continued basinwide assessment in 2018 to document any impacts from changing landuse in the watershed and to assess if the 2013 improvement to Excellent is the initiation of a trend or of a one-time fluctuation. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification FERRELS CR I SR 1525 105/16/131 BF43 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CHATHAM 1 4 1 03030002 1 35.8268271 -79.191177 1 16-32 Carolina Slate Belt Stream Classification Drainage Area (mi) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site W S-IV; NSW 1 15.7 1 362 1 6 0.3 1 Yes Forested/Wetland Urban Visible Landuse (%) 100 1 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) Agriculture Other (describe) 1 0 1 0 NPDES Number Volume (Q., MGD) None I --- I --- Water Quality Parameters Temperature (°C) 17.3 Dissolved Oxygen (mg/L) 7.3 Specific Conductance (pS/cm) 122 pH (s.u.) 6.6 Water Clarity I Slightly turbid Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 12 3 3 6 5 7 10 5 5 61 Substrate lBoulders, sand Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 05/16/13 2013-29 20 46 Good 05/15/08 2008-39 22 54 Excellent 03/13/08 2008-01 25 38 Fair 05/02/03 2003-14 15 48 Good 04/21/98 98-17 20 44 Good -Fair Eastern Mosquitofish and Redbreast Most Abundant Species, 2013 Sunfish (n=36 (16%) and 31 (14%), Exotic Species Green Sunfish (n=22) respectively) Gained -- Comely Shiner (n=4) and Margined Madtom first collections ever; and Golden Shiner (n=4). Lost -- Species Change Since Last Cycle Highfin Shiner, Creek Chub, White Sucker, Flat Bullhead, Redear Sunfish, Largemouth Bass (n=4), and Piedmont Darter. Unless otherwise noted, all species gained or lost were represented by 1-3 fish/species. uaca Watershed -- drains northern Chatham and southern Orange counties; tributary to the Haw River, no municipalities or NPDES dischargers in the watershed; site is — 0.5 mile upstream of the creek's confluence with the river. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories > 5% are reported) -- 79% forest and 11 % cultivation; since 1992 forested land has decreased from 88% to 79% and developed land has increased from < 1 % to 3%; greatest percentage of forested land of any fish community site in the basin that was assessed in 2013. Habitat -- watershed borders the Triassic Basins, instream and riparian habitats are more characteristic of that ecoregion than of the Carolina Slate Belt; sandy and gravely runs; infrequent cobble riffles; boulder crevices and pools; riparian zones are wide, intact, and well vegetated providing a good canopy over the stream; low flow. Water Quality -- specific conductance has gradually increased from 83 pS/cm in 1998 to 122 pS/cm in 2013. 2013 -- an 8 point decline in NCIBI score and 1 rating class decline resulted from the number of fish declining by 33% between 2008 and 2013 (329 vs. 219), the loss of the intolerant Piedmont Darter, and a moderately high percentage of tolerant fish (49%, Eastern Mosquitofish, Redbreast Sunfish, Green Sunfish, Satinfin Shiner, and Golden Shiner). 1998-2013 -- very diverse community for a stream of its size which is related to site's proximity to the river with species migrating to and from the river; 31 species are known from this site including 12 species of minnows, 8 species of sunfish, 2 species of darters, 1 intolerant species, and 4 nonindigenous species; total species diversity has declined by 5 species and number of fish has declined by 75% since 2008; dominant species is the Sandbar Shiner (31 %, although 74% of them were collected in March 2008); due to the site's proximity to the river and fluctuating water levels, the community and ratings are temporally variable. 2008 drought recovery studies were summarized in BAU Memorandum F-20090122. Recommendation -- based upon all the data collected, there may be a slight decline in water quality in the Ferrells Creek watershed (increase in specific conductance, a 8 point decline in NCIBI score, and 1 rating class change), but community may also be affected by flow extremes and proximity to the river; continued basinwide assessment of this regional reference site in 2018 is recommended to document any potential impacts from increased development in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification FORK CR SR 1003 105/14/131 BF23 I Excellent County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion RANDOLPH 9 1 03030003 1 35.5278451 -79.642261 1 17-25 Carolina Slate Belt Stream Classification Drainage Area (mi) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C 31.2 1 361 12 0.4 1 Yes Forested/Wetland Urban Visible Landuse (%) 90 1 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) Agriculture Other (describe) 10 0 NPDES Number Volume (Q., MGD) None I --- Water Quality Parameters Temperature (°C) Dissolved Oxygen (mg/L) P6.q7 Specific Conductance (pS/cm) pH (s.u.) Water Clarity I Clear, tannic stained Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 18 12 16 10 7 7 10 5 4 94 Substrate I Boulder, cobble, bedrock, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 05/14/13 2013-23 1 17 58 Excellent 06/08/09 2009-57 24 52 Good 06/26/03 2003-37 23 50 Good Most Abundant Species, 2013 Species Change Since Last Cycle uaca Highfin Shiner (n=116, 39%) Exotic Species Green Sunfish and Roanoke Bass (n= 13 and 6, respectively). Gained -- Chain Pickerel (n=1), Roanoke Bass (n=6), and Carolina Darter (n=4) first collections ever; and Speckled Killifish. Lost -- American Eel, Golden Shiner, Redlip Shiner (n=4), Spottail Shiner, Swallowtail Shiner (n=4), Sandbar Shiner, Fathead Minnow, Brassy Jumprock, Notchlip Redhorse, Flat Bullhead, and Fantail Darter. Unless otherwise noted, all species gained or lost were represented by 1-3 fish/species. Watershed -- drains southern Randolph and northern Moore counties; Town of Seagrove is in the headwaters; no NPDES dischargers in the watershed; tributary to the Deep River, site is — 3.9 miles above the stream's confluence with the river. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 60% forest, 25% cultivation, and 6% grassland/herbaceous; since 1992 forested land has decreased from 81 % to 60% and cultivated land from 17% to 25%; least amount of developed land of any fish community site in the basin that assessed in 2013. Habitat -- no change in habitats since 2003; highest total habitat score of any fish community site in the basin; typical high -quality Carolina Slate Belt -type stream -- slick angular rocks covered with periphyton; side root snags; long pools with cobble bottoms; cobble runs and riffles; boulders and bedrock; Podostemum in the riffles and Justicia (especially at the bridge); low flow. Water Quality -- specific conductance stable, ranging from 89 pS/cm in 2003 to 100 pS/cm in 2013. 2013 -- greatest NCIBI score (58) and most species of darters (n=3) collected from any site in the basin; only metric not scored the maximum value (5) was the Number of Species of Suckers (only one species was collected and was scored a'3"); increased NCIBI score and rating was due to a greater Percentage of Piscivores and more species with multiple age classes collected in 2013 than in 2009. 2004-2013 -- very diverse community with 31 species known from the site, including 12 species of minnows, 4 species each of catfish, darters (including the Carolina Darter, Etheostoma collis, a state Special Concern Species), and nonindigenous species, and 3 species of suckers; dominant species are the White Shiner and Highfin Shiner (14% each) and Tessellated Darter (13%); Percentage of Tolerant Fish stable (16%-23%); trophic metrics are stable (20%-28% Omnivores+Herbivores and 72%-80% Insectivores). In addition, Roanoke Bass, Ambloplites cavifrons, a Significantly Rare species in its native range in the Tar and Neuse River basins, was also collected in Fork Creek; it was introduced in the mid -late 1970s in the Deep River system to serve as a refugial population by the NC Wildlife Resources Commission. This community is affected more so by low flows and droughts than by changes in water quality; recurring and prolonged droughts most likely have prevented the community from being consistently rated Excellent. Recommendation -- in general, no long-term change in water quality in the Fork Creek watershed; continued basinwide assessment of this regional reference site in 2018. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification HAW CR SR 2158 104/18/131 BF55 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion ALAMANCE 2 1 03030002 1 6,0003619-79.342724 16-20-(4) Carolina Slate Belt Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C;NSW 1 28.0 1 462 1 9 1 0.4 1 No Forested/Wetland Rural Residential Agriculture Other (describe) Visible Landuse (%) 75 1 5 20 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None I --- I --- Water Quality Parameters Temperature (°C) R6.6 Dissolved Oxygen (mg/L) Specific Conductance (pS/cm) pH (s.u.) Water Clarity IClear Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 14 3 5 8 2 6 10 3 5 61 Substrate Gravel, sand, silt, boulders, cobble. Sample Date Sample ID Species Total NCIBI Score NCIBI Rating 04/18/13 2013-06 22 44 Good -Fair 04/13/09 2009-09 23 48 Good 04/23/03 2003-09 22 52 Good Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Spottail Shiner (n=144, 33%) Exotic Species Rosefin Shiner, Crescent Shiner, and Green Sunfish (n=63, 32, 9, respectively). Gained -- Highfin Shiner, Satinfin Shiner (n=12), Whitefin Shiner, and sunfish hybrid first collections ever; and Snail Bullhead. Lost -- Sandbar Shiner, Fathead Minnow, Creek Chub, and Oriental Weatherfish. Unless otherwise noted, all species gained or lost were represented by 1-3 fish/species. Watershed -- drains east -central Alamance and western Orange counties; upper watershed is traversed by the 1-85 corridor; no NPDES dischargers in the watershed; tributary to the Haw River, site is - 1.2 miles above the creek's confluence with the river; borders the Southern Outer Piedmont. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.htm], only landuse categories >_ 5% are reported) -- 51 % forest, 26% cultivation, and 14% developed, and 6% grassland/herbaceous; since 1992 forested land has decreased from 64% to 51 % and developed land has increased from 9% to 14%. Habitat -- snags and large deadfalls along the margins, runs, boulder outcrops and crevices on the right, gravel riffles and chutes; pools and deep water snags filled with gravel, silt, and sand; entrenched with red clay banks. Water Quality -- specific conductance has gradually increased since 2003, ranging from 86 µS/cm in 2003 to 120 pS/cm in 2013. 2013 -- most species (n=22) and greatest percentage of Omnivores+Herbivores (41 %) collected from any site in the basin; slight decrease in the NCIBI score and rating due to an abundance of the omnivorous Spottail Shiner which may have migrated upstream from the Haw River. 2003-2013 -- most diverse fish community site of any in the basin with 33 species known from the site, including 13 species of cyprinids and 7 nonindigenous species, but no intolerant species; dominant species are the Spottail Shiner (21 %) and Bluegill (14%); an 8-point decrease in the score and rating between 2003 and 2013 and an increase in the specific conductance may signal a true decline in water quality due to changing landuse practices. Recommendation -- continued basinwide assessment in 2018 to document any continuing impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification HECTOR CR SR 1412 108/13/131 BF40 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion HARNETT 7 1 03030004 1 35.4668211 -78.8583038 18-15-(0.7) Northern Outer Piedmont Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-IV;HQW 1 17.0 1 133 1 8 1 0.3 1 No Forested/Wetland Rural Residential Agriculture Other (describe) Visible Landuse (%) 50 1 50 0 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None I --- I --- Water Quality Parameters Temperature (°C) 24.3 Dissolved Oxygen (mg/L) 6.9 Specific Conductance (pS/cm) 73 pH (s.u.) 6.9 Water Clarity I Clear -tannic Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 16 11 14 8 6 6 10 3 3 82 Substrate lBedrock, sand, cobble, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Rating 08/13/13 2013-49 16 44 Good -Fair 06/06/03 2003-21 20 56 Excellent 05/06/98 98-32 20 46 Good Most Abundant Species, 2013 Exotic Species White Shiner (n=43, 28%) Green Sunfish, Redear Sunfish, and Spotted Bass, n=1 each) Species Change Since Last Cycle Data Analysis Gained -- Longnose Gar, Comely Shiner (n=10), and Redfin Pickerel first collections ever. Lost -- Whitefin Shiner (n=5), Creek Chubsucker, Spotted Sucker (n=8), Notchlip Redhorse, Largemouth Bass (young -of -year only), Tessellated Darter (n=9), and Piedmont Darter. Unless otherwise noted, all species gained or lost were represented by 1 or 2 fish/species. Watershed -- drains extreme southwestern Wake and northern Harnett counties; no municipalities or NPDES dischargers in the watershed; tributary to the Cape Fear River, site is - 1.7 miles from the creek's confluence with the river. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.htmi, only landuse categories >_ 5% are reported) -- 49% forest, 22% cultivation, 6% developed, 5% shrubland, and 15% grassland/herbaceous; since 1992 forested land has decreased from 61 % to 49%, developed land has increased from < 1 % to 6%, and cultivated land has decreased from 33% to 22%. Habitat -- very low flow at the time of sampling even though the wet spring and summer had resulted in earlier high flows; high quality instream habitats; bedrock riffles and runs, shallow pools, side snags, and undercuts; evidence of high water with eroded banks; Podostemum on the bedrock; narrow riparian zones. Water Quality -- specific conductance low, but increasing slightly, ranging from 48 pS/cm in 1998 to 73 pS/cm in 2013. 2013 -- loss of darter and sucker species and fewer species with multiple age classes (resulting from extremes in flows) resulted in a substantial decrease in the NCIBI score and rating; 1 of only 2 sites in the basin from which no darters were collected. 1998-2013 -- 29 species known from the site, including 2 species of darters, 1 intolerant species, and 3 nonindigenous species; dominant species are Bluehead Chub (28%) and White Shiner (18%); number of fish collected has always been unexplainably lower than expected; was not sampled in 2009 because of high turbid flows. Recommendation -- based upon all the data collected, there may be a decline in water quality in the Hector Creek watershed (increase in specific conductance, 12 point decline in NCIBI score, and 2 rating class change), but community may also be affected by flow extremes; continued basinwide assessment in 2018 to document any potential impacts from changing landuse in the watershed; due to changing landuse, site should no longer be considered a regional reference site. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification INDIAN CR I SR 2306 105/15/131 BF59 I Poor (Not Rated) County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CHATHAM 1 10 1 03030003 1 35.538333331 -79.33583333 17-35 Triassic Basins Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-IV 25.3 1 222 10 0.3 1 No Forested/Wetland Urban Visible Landuse (%) 50 1 0 Upstream NPDES Dischargers (i' 1 MGD or < 1 MGD and within 1 mile) Agriculture Other (describe) 50 1 0 NPDES Number Volume (Q., MGD) None I --- Water Quality Parameters Temperature (°C) 17.1 Dissolved Oxygen (mg/L) 7.8 Specific Conductance (pS/cm) 140 pH (s.u.) 6.7 Water Clarity I Slightly turbid Habitat Assessment Scores (max) Channel Modification (5) 5 Instream Habitat (20) 8 Bottom Substrate (15) 4 Riffle Habitat (16) 3 Pool Variety (10) 6 Erosion (7) 4 Bank Vegetation (7) 6 Light Penetration (10) 7 Left Riparian Score (5) 1 Right Riparian Score (5) 2 Total Habitat Score (100) 46 Sample Date Sample ID Site Photograph Substrate I Gravel, sand, fine silt and clay Species Total NCIBI Score NCIBI Rating 05/15/13 1 2013-26 9 24 Poor (Not Rated) 04/26/05 1 2005-35 22 52 Good 06/13/03 2003-26 15 36 Fair 04/23/98 98-22 22 56 Excellent Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Eastern Mosquitofish (n=147, 70%) Exotic Species Green Sunfish (n=6) Gained --Golden Shiner. Lost -- Whitefin Shiner (n=10), Whitemouth Shiner, Highfin Shiner (n=25), Comely Shiner (n=9), Spottail Shiner (n=33), Coastal Shiner, Sandbar Shiner (n=43), Creek Chubsucker (n=37), Notchlip Redhorse, Yellow Bullhead, Pirate Perch, Pumpkinseed (n=20), sunfish hybrid, Largemouth Bass, and Piedmont Darter (n=10). Unless otherwise noted, all species gained or lost were represented by 1-3 fish/species. Watershed -- drains southeastern Chatham County; no NPDES dischargers or municipalities in the watershed; tributary to the Deep River, site is — 1.8 miles upstream from the creek's confluence with the river; although the site is within the Triassic Basins, most of its watershed is in the Carolina Slate Belt ecoregion. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories Z 5% are reported) -- 58% forest, 27% cultivation, 5% grassland/herbaceous, and 5% shrubland; since 1992 forested land has decreased from 80% to 58% and cultivated land has increased from 17% to 27%%. Habitat -- variable flows from extremely high to extremely low; low flow at time of sampling; overall decline in the habitats during the past several cycles; total habitat score declined from 76 in 1998 to 46 in 2013; very silty, especially along the left bank; shallow; a couple of deeper pools with snags; embedded shallow gravel riffles; cattle with access resulting in breaks in the riparian zones, especially along the left bank; cattle barricade damaged by high flow. Water Quality -- specific conductance has increased over time from 91 pS/cm in 1998 to 140 pS/cm in 2013. 2013 -- 2nd fewest species and greatest percentage of tolerant fish (90%) collected at any site in the basin; between 2005 and 2013 the number of fish declined by 63% (564 vs. 209) and number of species declined by 59% (22 vs. 9), the greatest percentage loss of species at any site in the basin; 15 species representing 199 fish collected in 2005 were not collected in 2013; suckers, intolerant species, and piscivores absent; very skewed trophic structure (95% Insectivores and 5% Omnivores+Herbivores); only 2 out of 9 species were represented by multiple age classes (a very low percentage of species with multiple age classes is often seen in communities recovering from drought and high flow effects); a substantial decline in the NCIBI score and rating. 1998-2013 -- historically a diverse community with 30 species known from the site including 2 species of darters, 1 intolerant species, and 2 nonindigenous species; dominant species is the tolerant Eastern Mosquitofish (31 %); since 1998 there have been substantial changes in the fish community: 1) the percentage of Eastern Mosquitofish has increased from to 2% to 12% to 35% to 70% of all the fish collected, 2) the Percentage of Tolerant fish has increased from 18% to 36% to 52% to 90%, 3) the Percentage of Omnivores+Herbivores has decreased from 18% to 10% to 13% to 5%, and 4) the Percentage of Insectivores has increased from 80% to 82% to 87% to 95%. Recommendation -- continue monitoring this site in 2018 to document impacts from changing landuse practices, consider re -sampling in 2013 under more stable flow conditions to verify its 2013 rating and possibly prevent its placement on the impaired stream's list; until verified, community should be considered Not Rated in 2013. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification JORDAN CR SR 1754 104/22/131 BF46 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion ALAMANCE 2 03030002 1 36,188911-79.394761 16-14-6-(0.5) Southern Outer Piedmont Stream Classification Drainage Area (mi2) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-II;HQW,NSW 24.1 554 7 0.3 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 50 0 50 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q., MGD) None I --- Water Quality Parameters Temperature (°C) 13.0 Dissolved Oxygen (mg/L) 9.9 Specific Conductance (pS/cm) 98 pH (s.u.) 6.8 Water Clarity I Slightly turbid Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 9 3 2 8 4 7 8 5 5 56 Substrate Sand Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 04/22/13 2013-09 9 42 Good -Fair 06/03/09 2009-52 12 46 Good 04/23/03 2003-07 12 40 Good -Fair Most Abundant Species, 2013 Bluegill (n=14, 23%) Exotic Species None; 1 of only 2 sites where no nonindigenous species were collected. Species Change Since Last Cycle Gained -- Pirate Perch (n=1) first collection ever. Lost -- Golden Shiner (n=5), Highfin Shiner (n=22), Green Sunfish (n=18), and Redear Sunfish (n=1). Data Analysis -- drains primarily rural, northern Alamance and southern Caswell counties; no municipalities or NPDES dischargers within the watershed; tributary to Stony Creek Reservoir. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 47% forest, 38% cultivation, and 6% developed; since 1992 forested land has decreased from 64% to 47% and developed land has increased from 1 % to 6%. Habitat -- low flow; very shallow, sandy runs; infrequent stick riffles; abundant deadfalls and coarse woody debris; side snags and undercut pools; sun -lit in places; good riparian zones, however, the stream suffers from substantial bank erosion and habitat degradation; stream may go dry during droughts and may be impacted by prolonged low flow conditions. Water Quality -- specific conductance has ranged from 82 pS/cm in 2003 to 123 pS/cm in 2009. 2013 -- fewest fish (n=60) collected from any site in the basin; slight decrease in the NCIBI score and rating due to the loss of three species. 2003-2013 -- low diversity community for a stream of its size, only 15 species known from the site, including the Carolina Darter, Etheostoma collis, a state Special Concern Species, which was collected in 2003, 2009, and 2013, and the nonindigenous Green Sunfish and Redear Sunfish; intolerant species absent; Bluehead Chub, a ubiquitous Piedmont species is absent from the site; recolonization following droughts or prolonged low flows is affected by limited source populations in tributary streams because Jordan Creek is bracketed upstream by Lake Burlington and downstream by Stony Creek Reservoir; dominant species is Bluegill (23%). Recommendation -- because the fish community is strongly affected by low flows and limited source populations for recovery, basinwide monitoring could be conducted once every 10 years to track any impacts from long-term changes in land use patterns; based upon all the data collected there has been no long-term change in water quality in the Jordan Creek watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification KENNETH CR SR 1441 07/31/14 BF42 Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion HARNETT 7 03030004 1 35.514518-79.786129 18-16-1-(2) Northern Outer Piedmont Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WSW 1 15.4 1 220 1 8 1 0.4 1 No Forested/Wetland Urban Visible Landuse (%) 70 1 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) Agriculture Other (describe) 25 5 (sewer line right-of-way) NPDES Number Volume (Q., MGD) None --- --- Water Quality Parameters Temperature (°C) 25.0 Dissolved Oxygen (mg/L) 5.3 Specific Conductance (pS/cm) 89 pH (s.u.) 6.5 Water Clarity I Clear -slightly turbid Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) Sample Date 5 10 3 7 6 5 6 10 5 3 60 Sample ID Substrate Sand, silt, clay, gravel, cobble, bedrock Species Total NCIBI Score NCIBI Rating 07/31 /14 2014-69 19 40 Good -Fair 08/13/13 2013-48 14 34 Fair (Not Rated) 06/10/09 2009-62 15 40 Good -Fair 06/06/03 2003-20 15 46 Good 05/06/98 98-31 14 44 Good -Fair 02/09/94 94-01 12 34 Fair Most Abundant Species, 2013 Redbreast Sunfish (n=29, 32%) Exotic Species Green Sunfish, Spotted Bass, and Redear Sunfish, n = 2, 2, and 1, respectively. Species Change Since Last Cycle Gained -- Flier (n=1), first collection ever; Largemouth Bass (n=2). Lost -- Creek Chub (n=12), Eastern Mosquitofish (n=13), and Warmouth (n=4). Data Analysis Watershed -- drains northern Harnett and southern Wake counties, including the Town of Fuquay-Varina; no NPDES dischargers in the watershed, site was - 4.5 miles below the Kenneth Creek W WTP (NC002818, Qw = 1.2 MGD) which ceased discharging since 07/2007 after tying into Harnett County's W WTP (NC0021636); headwaters are in the Rolling Coastal Plain, although site is in the Northern Outer Piedmont; tributary to Neil's Creek. Landuse 2006 (from USGS, http://water.usgs.gov/osw/strearnstats/north_carolina.htm1, only landuse categories >_ 5% are reported) -- 31 % forest, 23% cultivation, 24% developed, and 15% grassland/herbaceous; since 1992 forested land has decreased from 41 % to 31 %, cultivated land from 42% to 23%, and developed land has increased from 14% to 24%. Habitat -- habitats appeared the same as in previous cycle; primarily runs, some side snags; gravel riffles; one artificial cobble riffle at sewer pipe which intersects the channel; sewer right-of-way within the right riparian zone; evidence of very high flows from the persistently and extremely high flows during the spring and summer. Water Quality -- specific conductance stable, ranging from 83 pS/cm in 1998 to 97 pS/cm in 2003; dissolved oxygen, recorded early in the morning, only at 64% of saturation. 2013 -- 6 point decline in NCIBI score and 1 rating class decline resulted from the number of fish declining by 67% between 2009 and 2013 (275 vs. 90), very low percentage of species with multiple age classes (29%) which is often seen in communities recovering from drought and high flow effects. 1994-2013 -- 23 species are known from the site, including 7 species of sunfish and 3 nonindigenous species, but no intolerant species or Notropis species (e.g., Sandbar Shiner, Highfin Shiner, Dusky Shiner, or Swallowtail Shiner) and suckers are generally absent; compared with reference sites, there is consistently lower than expected total species diversity; last two cycles have had skewed trophic metrics with too few Omnivores+Herbivores (4% and 9%) and and high percentages of Insectivores (88%) and Piscivores (2.6% and 7.8%); abundance of Bluehead Chub has declined sharply since 1994 (4% in 2013 and 55%) in 1994). Recommendation -- based upon all the data collected, there may be a slight decline in water quality in the watershed (6-12 point decline in NCIBI score and 2 rating class changes since 2003), continue monitoring in 2018 to document impacts from changing landuse practices; consider re -sampling in 2014 under more stable flow conditions to verify its 2013 rating and possibly prevent its placement on the impaired stream's list; until verified, community should be considered Not Rated in 2013. Note: the site was re -sampled in 2014 and was rated Good -Fair; one new species was collected (Creek Chubsucker). FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification KENNETH CR SR 1441 108/13/131 BF42 I Fair (Not Rated) County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion HARNETT 1 7 03030004 1 35.5145181 -79.786129 18-16-1-(2) Northern Outer Piedmont Stream Classification Drainage Area (mi) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-IV 15.4 1 220 1 8 0.4 1 No Forested[Wetland Urban Agriculture Other (describe) Visible Landuse (%) 70 1 0 25 5 (sewer line right-of-way) Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None Water Quality Parameters Temperature (°C) 25.0 Dissolved Oxygen (mg/L) 5.3 Specific Conductance (pS/cm) 89 pH (s.u.) 6.5 Water Clarity I Clear -slightly turbid Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) Sample Date 5 10 3 7 6 5 6 10 5 3 60 Sample ID Substrate ISand, silt, clay, gravel, cobble, bedrock Species Total NCIBI Score NCIBI Rating 08/13/13 2013-48 14 34 Fair (Not Rated) 06/10/09 2009-62 15 40 Good -Fair 06/06/03 2003-20 15 46 Good 05/06/98 98-31 14 44 Good -Fair 02/09/94 94-01 12 34 Fair Most Abundant Species, 2013 Redbreast Sunfish (n=29, 32%) Exotic Species Green Sunfish, Spotted Bass, and Redear Sunfish, n = 2, 2, and 1, respectively. Species Change Since Last Cycle Gained -- Flier (n=1), first collection ever; Largemouth Bass (n=2). Lost -- Creek Chub (n=12), Eastern Mosquitofish (n=13), and Warmouth (n=4). uaca Watershed -- drains northern Harnett and southern Wake counties, including the Town of Fuquay-Varina; no NPDES dischargers in the watershed, site was — 4.5 miles below the Kenneth Creek WWTP (NC002818, Qw = 1.2 MGD) which has been off-line since 07/2007 after tying into Harnett County's WWTP (NC0021636); headwaters are in the Rolling Coastal Plain, although site is in the Northern Outer Piedmont; tributary to Neil's Creek. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.htmi, only landuse categories >_ 5% are reported) -- 31 % forest, 23% cultivation, 24% developed, and 15% grassland/herbaceous; since 1992 forested land has decreased from 41 % to 31 %, cultivated land from 42% to 23%, and developed land has increased from 14% to 24%. Habitat -- habitats appeared the same as in previous cycle; primarily runs, some side snags; gravel riffles; one artificial cobble riffle at sewer pipe which intersects the channel; sewer right-of-way within the right riparian zone; evidence of very high flows from the persistently and extremely high flows during the spring and summer. Water Quality -- specific conductance stable, ranging from 83 pS/cm in 1998 to 97 pS/cm in 2003; dissolved oxygen, recorded early in the morning, only at 64% of saturation. 2013 -- 6 point decline in NCIBI score and 1 rating class decline resulted from the number offish declining by 67% between 2009 and 2013 (275 vs. 90), very low percentage of species with multiple age classes (29%) which is often seen in communities recovering from drought and high flow effects. 1994-2013 -- 23 species are known from the site, including 7 species of sunfish and 3 nonindigenous species, but no intolerant species or Notropis species (e.g., Sandbar Shiner, Highfin Shiner, Dusky Shiner, or Swallowtail Shiner) and suckers are generally absent; compared with reference sites, there is consistently lower than expected total species diversity; last two cycles have had skewed trophic metrics with too few Omnivores+Herbivores (4% and 9%) and and high percentages of Insectivores (88%) and Piscivores (2.6% and 7.8%); abundance of Bluehead Chub has declined sharply since 1994 (4% in 2013 and 55%) in 1994). Recommendation -- based upon all the data collected, there may be a slight decline in water quality in the Kenneth Creek watershed (6-12 point decline in NCIBI score and 2 rating class changes since 2003), continue monitoring this site in 2018 to document impacts from changing landuse practices; consider re -sampling in 2014 under more stable flow conditions to verify its 2013 rating and possibly prevent its placement on the impaired stream's list; until verified, community should be considered Not Rated in 2013. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification LITTLE ALAMANCE CR I SR 2309 104/24/131 BF60 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion ALAMANCE 3 1 03030002 1 36.0346451 -79.408991 1 16-19-11 Southern Outer Piedmont Stream Classification Drainage Area (mi) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C;NSW 14.5 1 485 1 8 0.4 1 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 25 50 0 25 (park) Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q., MGD) None --- --- Water Quality Parameters Temperature (°C) 15.4 Dissolved Oxygen (mg/L) 9.2 Specific Conductance (pS/cm) 184 pH (s.u.) 7.1 Water Clarity I Slightly turbid Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) Sample Date 5 12 3 5 8 3 6 9 3 2 56 Sample ID Site Photograph Substrate lCobble, boulder, gravel, sand Species Total NCIBI Score NCIBI Rating 04/24/13 2013-15 20 44 1 Good -Fair 04/16/09 2009-15 21 50 Good 04/23/03 2003-08 20 52 Good 04/08/98 98-12 10 38 Fair 11 /04/93 93-41 14 48 Good F osefin Shiner, Crescent Shiner, Green Sunfish, Most Abundant Species, 2013 Redbreast Sunfish (n=86, 38%) Exotic Species edear Sunfish, White Sucker (n=16, 3, 2, 1, and respectively) Species Change Since Last Cycle Gained -- Yellow Bullhead (n=1) and sunfish hybrid (n=2) first collections ever; and Creek Chubsucker (n=2). Lost -- Eastern Mosquitofish (n=6), Pumpkinseed (n=2), and Warmouth (n=20). uaca Watershed -- drains the City of Burlington and the 1-85/1-40/US 70 corridors in central Alamance County; no NPDES dischargers in the watershed; borders the Carolina Slate Belt with its characteristic stream qualities (i.e. rocky substrates and very low summer flows); site is —2.6 miles above the creek's confluence with Big Alamance Creek. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 11 % forest and 84% developed; since 1992 forested land has decreased from 19% to 11 % and developed land has increased from 72% to 84%; greatest percentage of developed land and least percentage of forested land of any fish community site in the basin that was assessed in 2013. Habitats -- urban debris in the channel, along the banks, and in the flood plain; hydrologically flashy due to its developed watershed; severe sloughing of the right bank near the beginning of the reach. Water Quality -- elevated specific conductance, 156-186 pS/cm, is indicative of stormwater and nonpoint source runoff. 2013 -- 6 point decline in NCIBI score and 1 rating class decline resulted from the number of fish decreasing by 37% between 2009 and 2013, a very low percentage of species with multiple age classes which is often seen in populations recovering from drought or high flow effects. In addition, there was a moderately high percentage of diseased fish (1.79%, Bluegill with popeye disease). 2004-2013 -- a very diverse stream for its size and considering the very high percentage of its watershed which is developed; 28 species known from the site, including 6 species of sunfish and 5 nonindigenous species, but no intolerant species. Dominant species is the Tessellated Darter (23%) which was uncommon in 1993 and 1998; over the past three cycles the diversity (total species, species of darters, sunfish, and suckers) and trophic metrics (Omnivores+Herbivores and Insectivores) have been stable, but the Number of Fish has declined by 56% (515 vs. 224) and the Percentage of Tolerant Fish has increased (19% to 30% to 43%); since 2003 the NCIBI score has declined by 8 points and 1 rating class. Recommendation -- based upon all the data collected, there may be a slight decline in water quality in the Little Alamance Creek watershed (6-8 point decline in NCIBI score and 1 rating class change), but community may also be more affected by recent flow extremes than by changes in water quality. It is recommended that to continue basinwide assessment of this site in 2018 to document any potential impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification LITTLE ALAMANCE CR SR 3039 104/23/131 BF67 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion GUILFORD 3 03030002 1 36,029791-79.719191 16-19-3-(0.5) Southern Outer Piedmont Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-IV;NSW 1 10.4 1 661 1 6 1 0.4 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 80 0 20 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None I --- I --- Water Quality Parameters Temperature (°C) 13.0 Dissolved Oxygen (mg/L) 8.6 Specific Conductance (pS/cm) 200 pH (s.u.) 7.0 Water Clarity Slightly turbid, greenish Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 14 3 7 8 4 7 10 4 5 67 Substrate Gravel, sand, cobble Sample Date Sample ID Species Total NCIBI Score NCIBI Rating 04/23/13 2013-11 13 44 Good -Fair 04/16/09 2009-13 13 44 Good -Fair 04/22/03 2003-05 13 44 Good -Fair 04/22/98 98-18 14 44 Good -Fair Most Abundant Species, 2013 IBluehead Chub (n=135, 33%) 1Exotic Species lWhite Sucker (n=2) Species Change Since Last Cycle Gained -- White Sucker (n=2) and Flat Bullhead (n=1). Lost -- Redear Sunfish (n=9) and Largemouth Bass (n=1) vaia Anarysis Watershed -- drains an urban/suburban area south and east of 1-85, US 421, and NC 22; one permitted discharger (Qw = 0.01 MGD) located - 4 miles upstream; tributary to Big Alamance Creek. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only Ianduse categories >_ 5% are reported) -- 41 % forest, 24% cultivation, and 26% developed; since 1992 forested land has decreased from 57% to 41 % and developed land has increased from 15% to 26%. Habitats -- beaverdams were observed upstream and downstream of the reach sampled in previous cycles, therefore the reach was moved further downstream from the 1-85 bypass bridge; habitats includes undercuts, snags, coarse woody debris and deadfalls; snags, boulder crevices, riffles, runs, sticks, periphyton-covered rocks; and forested riparian zones. Water Quality -- elevated specific conductance resulting from urban stormwater and nonpoint run-off, gradually increasing over time, ranging from 123 pS/cm in 1998 to 200 pS/cm in 2013. 2013 -- number of fish declined by 38% between 2009 and 2013 (659 vs. 410), but still an abundant community for an increasingly developed watershed; total species, species of suckers and darters consistently lower than expected. 1998-2013 -- 17 species known from the site, but no intolerant species and suckers are rare; dominant species is the Bluehead Chub (31 %); stable metrics for Number of Species, Percentage of Tolerant Fish (19%-31 %), Omnivores+Herbivores (24%-33%) and Insectivores (67%-75%); NCIBI scores stable and community has consistently rated Good -Fair. Recommendation -- continue monitoring this site in 2018 to document impacts from increasing urbanization of its watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification LITTLE TROUBLESOME CR SR 2600 104/22/131 BF63 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion ROCKINGHAM 1 1 1 03030002 1 36.282488-79,61162 16-7b Northern Inner Piedmont Stream Classification Drainage Area (mi) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C;NSW 12.1 1 651 1 5 0.2 1 No Forested/Wetland Rural Residential Agriculture Other (describe) Visible Landuse (%) 1 75 1 25 1 0 0 Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q., MGD) None I --- I --- Water Quality Parameters Temperature (°C) .9 Dissolved Oxygen (mg/L) .3 P6.6 Specific Conductance (pS/cm) 33 pH (s.u.) Water Clarity I Clear, easily silted Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 7 3 5 1 1 2 0 2 5 31 Substrate Isand, gravel, artificial cobble riffle Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 04/22/13 2013-07 11 40 Good -Fair 06/03/09 2009-50 15 44 Good -Fair 04/21 /03 2003-01 14 44 Good -Fair 10/12/98 98-82 9 24 Poor 04/06/98 98-04 11 36 Fair Most Abundant Species, 2013 Bluehead Chub (n=35, 32%) Exotic Species Crescent Shiner, Rosefin Shiner, and Green Sunfish (n=27, 4, and 3, respectively) Species Change Since Last Cycle Gained -- none. Lost -- Flat Bullhead (n=1), Eastern Mosquitofish (n=1), Pumpkinseed (n=1), Warmouth (n=4), land Largemouth Bass (n=7). Data Analysis Watershed -- drains southeastern Rockingham County, including the southeastern area of the Town of Reidsville; no NPDES dischargers in the watershed; borders the Southern Outer Piedmont; tributary to the Haw River, site is — 1 mile above its confluence with the river. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories > 5% are reported) -- 34% forest, 19% cultivation, and 41 % developed; since 1992 forested land has decreased from 45% to 34% and developed land has increased from 33% to 41 %. Habitat -- lowest rated habitat quality of any fish community site in the basin in 2013; no canopy, coarse woody debris, pools, or instream habitats; young willows and River Birch planted along the banks and in the riparian zones as a EEP stream restoration project (http://portal.ncdenr.org/c/document_library/get_file?p_I_id=60409&folderld=7037682&name=DLFE-64260.pdf); very shallow cobble and gravel runs; artificially constructed cobble/gravel riffle; periphyton abundant; low flow. Water Quality -- specific conductance has ranged from 112-139 pS/cm since 2003 2013, indicative of urban stormwater and nonpoint source runoff; before relocation of the upstream WWTP discharge it was 236 and 549 pS/cm. 2013 -- slight decline in NCIBI score was due to fewer fish and species of sunfish collected in 2013 than in 2009; number offish declined by 66% between 2009 and 2013 (316 vs. 106); greatest percentage of nonindigenous species (27%) and nonindigenous fish (32%) of any site in the basin. 1998-2013 -- 21 species are known from the site including 8 species of cyprinids and 3 nonindigenous species; no suckers, species of Notropis, or intolerant species have ever been collected at this site; dominant species are Bluegill (23%) and Bluehead Chub (22%); improvements in the fish community were documented after the discharge was relocated in 1998, but improvements have plateaued. Recommendation -- based upon all the data collected, there has been no recent change in water quality in the Little Troublesome Creek watershed; continued basinwide assessment in 2018 to document any improvements in the fish community resulting from the completed stream restoration project. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification LOVES CR SR 2229 107/31/141 BF58 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CHATHAM 12 03030003 35,729184 -79.428752 17-43-10b Carolina Slate Belt Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C 1 8.2 1 511 1 5 1 0.3 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 25 1 0 0 75 (Industrial - WWTP) Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None --- --- Water Quality Parameters Temperature (°C) 15.8 Dissolved Oxygen (mg/L) 9.3 Specific Conductance (pS/cm) 166 pH (s.u.) 6.9 Water Clarity I Clear, easily silted Habitat Assessment Scores (max) Channel Modification (5) 4 Instream Habitat (20) 14 Bottom Substrate (15) 8 Riffle Habitat (16) 10 Pool Variety (10) 8 Erosion (7) 6 Bank Vegetation (7) 7 Light Penetration (10) 10 Left Riparian Score (5) 3 Right Riparian Score (5) 5 Total Habitat Score (100) 75 Sample Date Sample ID Substrate Cobble, gravel, bedrock, boulder, and angular rocks Species Total NCIBI Score NCIBI Rating 07/31 /14 2014-68 21 44 Good -Fair 04/17/13 2013-02 13 32 Poor (Not Rated) 04/15/09 2009-11 19 52 Good 05/05/03 2003-17 21 44 Good -Fair 05/04/98 98-27 20 52 Good Most Abundant Species, 2013 Species Change Since Last Cycle Redbreast Sunfish (n=136, 49%) Exotic Species lGreen Sunfish (n=29) Gained -- Yellow Bullhead and Chain Pickerel (n=10) first collections ever; and Bluegill (n=48). Lost -- Swallowtail Shiner, Sandbar Shiner (n=11), Creek Chub, Brassy Jumprock, Speckled Killifish (n=6), Eastern Mosquitofish, Warmouth, Redear Sunfish, and Piedmont Darter (n=11). Unless otherwise noted, all species gained or lost were represented by 1-3 fish/species. ua[a Hnaiysis Watershed -- includes most of the Town of Siler City in western Chatham County; no NPDES dischargers in the watershed; tributary to the Rocky River, site is - 0.5 mile above the creek's confluence with the river and upstream from the WWTP's 4 MGD outfall. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 40% forest, 15% cultivation, and 39% developed; since 1992 forested land has decreased from 57% to 40% and developed land has increased from 30% to 39%. Habitat -- Carolina Slate Belt -type stream; very slick rocks with thick bluegreen algae mats (Phormidium amoenum ); deep pool at end of reach filled with soft sediment; similar appearing habitats as in 2009. Water Quality -- specific conductance has ranged from 107 µS/cm in 2003 to 166 pS/cm in 2013, increased by 44 µS/cm between 2009 and 2013; low flow. 2013 -- a 20 point NCIBI score and 3 rating class differences between 2009 and 2013 because of the loss of total species, loss of species of darters, sunfish, suckers, and intolerant species; a skewed trophic structure (5% Omnivores+Herbivores and 91 % Insectivores), and high percentages of Tolerant Fish (61 %) and diseased fish (2.9%, primarily Bluegill with popeye disease); recolonization would entail migrating from the river upstream through the effluent mixing zone to reach the site. 1998-2013 -- aside from the 2013 sample, an extremely diverse community for the size of the stream, 27 species are known from the site, including 2 species of darter, 1 intolerant species, and 2 nonindigenous species; site's proximity to the river influences overall diversity of the community with some species migrating to and from the river; the number of fish has declined by 45% since 2003 (507 vs. 332 vs. 276) and the number of species collected has declined since 2003; consistently high Percentage of Tolerant Fish (55-61 %); dominant species are the tolerant Redbreast Sunfish (37%) and Green Sunfish (18%). Recommendation -- continue monitoring this site in 2018 to document impacts from changing landuse practices in this increasingly urbanized watershed, consider re -sampling in 2013; until verified, community should be considered Not Rated in 2013. Note: the site was re -sampled in 2014 and was rated Good -Fair. The specific conductance was elevated (211 µS/cm); the flow was very low with many exposed riffles; 74% of all the fish collected were the tolerant Green Sunfish (38%), the tolerant Redbreast Sunfish (20%), and Bluegill (16%); most fish ever collected at this site (n=687); community continued to have a skewewd trophic structure (96% Insectivores and only 4% Omnivores+Herbivores); two new species collected (Pirate Perch and Notchlip Redhorse). FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification LOVES CR I SR 2229 104/17/131 BF58 I Poor (Not Rated) County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CHATHAM 12 1 03030003 1 35.7291841 -79.428752 17-43-10b Carolina Slate Belt Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C 8.2 1 511 1 5 0.3 1 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 25 0 0 75 (Industrial - WWTP) Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q„ MGD) None I --- Water Quality Parameters Temperature (°C) 15.8 Dissolved Oxygen (mg/L) 9.3 Specific Conductance (pS/cm) 166 pH (s.u.) 6.9 Water Clarity I Clear, easily silted Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 4 14 8 10 8 6 7 10 3 5 75 Substrate lCobble, gravel, bedrock, boulder, and angular rocks Sample Date SamDle ID Species Total NCIBI Score NCIBI Ratinq 04/17/13 2013-02 13 32 Poor (Not Rated) 04/15/09 2009-11 19 52 Good 05/05/03 2003-17 21 44 Good -Fair 05/04/98 98-27 20 52 Good Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Redbreast Sunfish (n=136, 49%) Exotic Species lGreen Sunfish (n=29) Gained -- Yellow Bullhead and Chain Pickerel (n=10) first collections ever; and Bluegill (n=48). Lost -- Swallowtail Shiner, Sandbar Shiner (n=11), Creek Chub, Brassy Jumprock, Speckled Killifish (n=6), Eastern Mosquitofish, Warmouth, Redear Sunfish, and Piedmont Darter (n=11). Unless otherwise noted, all species gained or lost were represented by 1-3 fish/species. Watershed -- includes most of the Town of Siler City in western Chatham County; no NPDES dischargers in the watershed; tributary to the Rocky River, site is — 0.5 mile above the creek's confluence with the river and upstream from the W WTP's 4 MGD outfall. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories > 5% are reported) -- 40% forest, 15% cultivation, and 39% developed; since 1992 forested land has decreased from 57% to 40% and developed land has increased from 30% to 39%. Habitat -- Carolina Slate Belt -type stream; very slick rocks with thick bluegreen algae mats (Phormidium amoenum ); deep pool at end of reach filled with soft sediment; similar appearing habitats as in 2009. Water Quality -- specific conductance has ranged from 107 pS/cm in 2003 to 166 pS/cm in 2013, increased by 44 pS/cm between 2009 and 2013; low flow. 2013 -- a 20 point NCIBI score and 3 rating class differences between 2009 and 2013 because of the loss of total species, loss of species of darters, sunfish, suckers, and intolerant species; a skewed trophic structure (5% Omnivores+Herbivores and 91 % Insectivores), and high percentages of Tolerant Fish (61 %) and diseased fish (2.9%, primarily Bluegill with popeye disease); recolonization would entail migrating from the river upstream through the effluent mixing zone to reach the site. 1998-2013 -- aside from the 2013 sample, an extremely diverse community for the size of the stream, 27 species are known from the site, including 2 species of darter, 1 intolerant species, and 2 nonindigenous species; site's proximity to the river influences overall diversity of the community with some species migrating to and from the river; the number of fish has declined by 45% since 2003 (507 vs. 332 vs. 276) and the number of species collected has declined since 2003; consistently high Percentage of Tolerant Fish (55-61 %); dominant species are the tolerant Redbreast Sunfish (37%) and Green Sunfish (18%). Recommendation -- continue monitoring this site in 2018 to document impacts from changing landuse practices in this increasingly urbanized watershed, consider re -sampling in 2013 under more stable flow conditions; until verified, community should be considered Not Rated in 2013. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification MARYS CR SR 2174 104/18/131 BF101 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion ALAMANCE 4 03030002 35,915973 79.306684 16-26 Carolina Slate Belt Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C;NSW 1 11.9 1 423 7 0.3 No Forested/Wetland Rural Residential Agriculture Other (describe) Visible Landuse (%) 1 85 1 15 0 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None I --- I --- Water Quality Parameters Temperature (°C) R6.8 Dissolved Oxygen (mg/L)Specific Conductance (pS/cm)pH (s.u.) Water Clarity I Clear, slightly stained Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 18 12 15 8 6 6 10 5 5 90 Substrate boulder, cobble, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Rating 04/18/13 2013-05 21 48 Good 04/13/09 2009-08 26 50 Good 06/09/04 2004-78 24 54 Excellent Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Bluehead Chub (n=140, 25%) Exotic Species Crescent Shiner, Green Sunfish, and White Sucker (n=95, 31, and 5, respectively) Gained -- Yellow Bullhead, first collection ever. Lost -- Rosefin Shiner (n=5), Golden Shiner, Brassy Jumprock, V-lip Redhorse (n=4), Pumpkinseed (n=3), and Largemouth Bass. Unless otherwise noted, all species gained or lost were represented by 1 fish/species. Watershed -- drains south-central Alamance County; no municipalities or NPDES dischargers in the watershed; tributary to the Haw River, site is — 1 mile above the creek's confluence with the river. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 48% forest, 38% cultivation, and 6% developed, and 6% grassland/herbaceous; since 1992 forested land has decreased from 62% to 48% and developed land has increased from <1 % to 6%. Habitats -- 3`d highest total habitat score of any fish community site in the basin; a typical Carolina Slate Belt -type stream with high quality instream and riparian habitats; variety of sizes and depths to the riffles and pools, very slick periphyton-covered rocks, root mats, and snags; low flow. Water Quality -- specific conductance relatively stable, ranging from 89 pS/cm in 2009 to 114 pS/cm in 2004. 2013 --unlike the similar size Pokeberry Creek, the number of fish collected has been very stable ranging from 553-581; slight decrease in the NCIBI score due to loss of a species of sunfish, however, no change in the rating. 2004-2013 -- very diverse community for a stream of its size which is related to site's proximity to the river with species migrating to and from the river; 32 species known from the site, including 13 species of cyprinids, 5 nonindigenous species, and 4 species of suckers, but no intolerant species; dominant species is Bluehead Chub (23%); trophic metrics are stable (24%- 34% Omnivores+Herbivores and 65%-76% Insectivores); affected more so by low flows and droughts as are many Carolina Slate Belt streams. Recommendation -- based upon all the data collected, there has been no change in water quality in the Marys Creek watershed; continued basinwide assessment in 2018 to document any impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification MCLENDONS CR SR 1210 109/04/131 BF103 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion MOORE 10 1 03030003 1 35,317009-79.543348 17-30 Triassic Basins Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C 114.3 1 350 1 6 1 0.3 1 Yes Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 100 0 0 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q„,, MGD) None I --- I --- Water Quality Parameters Temperature (°C) R5.9 Dissolved Oxygen (mg/L)Specific Conductance (pS/cm) pH (s.u.) Water Clarity I Clear -tannic Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 4 14 4 10 6 3 6 10 4 4 65 Substrate Gravel, sand, boulders at the end of the reach along the right bank Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 09/04/13 2013-52 17 46 Good 06/08/09 2009-58 13 46 Good 05/05/98 98-29 16 50 Good Most Abundant Species, 2013 Species Change Since Last Cycle ua[a Ana Highfin Shiner (n=96, 51 %) Exotic Species lGreen Sunfish (n=2) Gained -- Golden Shiner, Flier (n=5), Warmouth, and Bluegill (n=10) first collections ever; Margined Madtom and Redfin Pickerel. Lost -- Coastal Shiner, Creek Chubsucker (n=9), and Notchlip Redhorse (n=4). Unless otherwise noted, all species gained or lost were represented by 1-3 fish/species. Watershed -- drains central Moore County; no municipalities or NPDDES dischargers within the watershed; headwaters are in the Sand Hills, although the site is in the Triassic Basins, also borders the Carolina Slate Belt; large tributary to the Deep River; site is in the creek's headwaters. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.htm1, only landuse categories >_ 5% are reported) -- 62% forest, 7% cultivation, 5% developed, and 19% grassland/herbaceous; since 1992 forested land has decreased from 87% to 62% and developed land has increased from < 1 % to 5%. Habitats -- at time of sampling it was during a low flow period, but most of the spring and summer was a persistently and extremely high -flow period; decline in total habitat score from previous cycle due to effects from the high flows; eroded banks from the very high flows; coarse woody debris; gravel riffles; undercuts, snags; deep pool at end of the reach at site of an old mill; entrenched, possibly dredged and channelized historically. Water Quality -- lowest specific conductance of any fish community site in 2013; has ranged from 22pS/cm-38 pS/cm. 2013 -- lowest percentage of tolerant fish (10%) of any site in the basin; no change in score and ratings, but some metrics had decreased due to the persistent high flows (e.g., total number of fish and number of species with multiple age classes). 1998-2013 -- 24 species are known from the site, including 3 species of darters, 1 intolerant species, and only 1 nonindigenous species; dominant species is the Highfin Shiner (24%); despite extremes in flows, NCIBI scores and ratings have been consistently Good since 1998. Recommendation -- based upon all the data collected, there has been no long-term change in water quality in the McLendons Creek watershed; continued basinwide assessment of this regional reference site in 2018 to document any potential impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification MORGAN CR off SR 1900 105/10/131 BF15 I Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion ORANGE 6 03030002 35,892783 79.016389 16-41-2-(5.5)b Triassic Basins Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-IV;NSW 1 41.6 1 245 13 0.4 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 100 0 0 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) OWASA Mason Farm WWTP NCO025241 14.5 Water Quality Parameters Temperature (°C) R7.0 Dissolved Oxygen (mg/L)Specific Conductance (pS/cm)pH (s.u.) Water Clarity I Slightly turbid Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 4 7 4 3 8 5 7 9 5 5 57 Substrate Sand, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Rating 05/10/13 2013-17 22 38 Fair 04/20/99 99-20 23 38 Fair 05/18/98 98-37 19 36 Fair Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Eastern Mosquitofish (n=292, 51%) Exotic Species Channel Catfish, Green Sunfish, and Redear Sunfish (n=8, 6, and 2) Gained -- Flat Bullhead and Flier (n=1 each) first collections ever; Swallowtail Shiner (n=72), Gizzard Shad (n=7), Redfin Pickerel, (n=8) and Tessellated Darter (n=12). Lost -- Threadfin Shad (n=21), Rosyside Dace N=1), White Shiner (n=1), Bluehead Chub, Creek Chub (n=2), White Catfish (n=1), and Pumpkinseed (n=3). Watershed -- drains western and southern portions of the cities of Chapel Hill and Carrboro in southeastern Orange County; site is — 0.7 mile downstream from the Mason Farm WWTP; tributary to New Hope Creek and ultimately Jordan Reservoir. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 63% forest, 11 % cultivation, and 21 % developed; since 1992 forested land has decreased from 76% to 63% and developed land has increased from 12% to 21 %. Habitat -- well -shaded channel for the size of the stream, surrounded by wide riparian zones; hydrologically flashy due to its developed watershed. Water Quality -- 3rd greatest specific conductance of any fish community site in 2013 resulting from upstream WWTP effluent, has ranged from 219 pS/cm in 1999 to 320 pS/cm in 1998. 2013 - most species (n=22) and number of species of sunfish (n=7) collected from any site in the basin; very skewed trophic structure (94% Insectivores and 3% Omnivores+Herbivores), very high percentage of tolerant fish (71 % comprised of Redbreast Sunfish, Green Sunfish, Satinfin Shiner, Flat Bullhead, and Eastern Mosquitofish). 1998-2013 -- proximity to backwaters of Jordan Reservoir influences species diversity, especially that of the sunfishes, a very abundant and diverse community with 32 species known from the site, including 9 species of sunfish, 7 species of cyprinids, 5 nonindigenous species, but no intolerants, and only 1 individual of 1 species of sucker (Notchlip Redhorse) has ever been collected here; dominant species are the tolerant Redbreast Sunfish (28%) and Eastern Mosquitofish (26%). Recommendation -- based upon all the water quality and fish community data collected, this stream has consistently been impaired; future monitoring can be reduced to once every 10 years. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification N PR STINKING QUARTER CR SR 1129 104/24/131 BF135 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion ALAMANCE 3 03030002 35,993487 79.514609 16-19-8-1 Southern Outer Piedmont Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C;NSW 1 20.8 1 526 10 0.4 1 No Forested/Wetland Rural Residential Agriculture Other (describe) Visible Landuse (%) 1 60 1 20 20 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None I --- Water Quality Parameters Temperature (°C) 13.3 Dissolved Oxygen (mg/L) 8.7 Specific Conductance (pS/cm) 118 pH (s.u.) 6.7 Water Clarity I Clear, slightly stained Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 12 4 2 6 5 6 8 4 3 55 Substrate Sand, gravel, boulders along the right bank Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 04/24/13 2013-13 11 44 Good -Fair 06/04/09 2009-54 20 50 Good 04/24/03 2003-10 @ SR 1113 16 46 Good Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Redbreast Sunfish (n=38, 35%) Exotic Species JGreen Sunfish and White Sucker (n=9 and 1) Gained -- Creek Chubsucker. Lost -- Crescent Shiner (n=22), Whitemouth Shiner (n=5), Highfin Shiner, Creek Chub (n=13), Brassy Jumprock, Pirate Perch (n=5), Speckled Killifish (n=6), Pumpkinseed, Redear Sunfish, sunfish hybrid, and Black Crappie. Unless otherwise noted, all species gained or lost were represented by 1 or 2 fish/species. Watershed -- drains southeastern Guilford and western Alamance counties; tributary to Big Alamance Creek; no municipalities within the watershed; one small permitted discharger in the headwaters (NC0038164, Qw = 0.0045 MGD). Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.htmi, only landuse categories >_ 5% are reported) -- 43% forest, 43% cultivation, and 7% developed; since 1992 forested land has decreased from 58% to 43% and developed land has increased from 1 % to 7%. Habitats -- thick deposits of silts along the stream margins; boulder and gravel bottom pools; coarse woody debris in the channel; runs, snags, gravel bars, and stick riffles present; stream channel entrenched; low flow. Water Quality -- specific conductance has ranged from 86 pS/cm in 2003 to 123 pS/cm in 2009. 2013 -- 6 point decrease in NCIBI score and one rating class decline resulted from the loss of fish (declining by 75% between 2009 and 2013 (429 vs. 108), the greatest percentage at any site in the basin), the loss of species (declining by 45%, the second greatest loss in the basin), and from a low percentage of species with multiple age classes (which is often seen in communities recovering from drought and high flow effects). 2003-2013 -- 23 species known from the two sites, including 3 species of suckers, but no intolerant species; 4 nonindigenous species known from the two sites with the tolerant and nonindigenous Green Sunfish the dominant species (27%), which can become dominant in streams that are subject to recurring low flows; moderately high Percentage of Tolerant Fish (47% 55%), stable trophic metrics (Omnivores+Herbivores 10%-18% and Insectivores 81 %-90%); community seems to be affected by extreme flows more so than the adjacent South Prong Stinking Quarter Creek watershed which has similar landuse characteristics but a larger drainage area (33.6 mi). Recommendation -- based upon all the data collected, there may be a slight decline in water quality in the North Prong Stinking Quarter Creek watershed (6 point decline in NCIBI score and 1 rating class change), but community may also be affected by flow extremes than by changes in water quality; continued basinwide assessment of this site in 2018 to document any potential impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification N BUFFALO CR SR 2770 105/13/131 BF66 I Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion GUILFORD 2 03030002 1 36,129797-79.662723 16-11-14-1b Southern Outer Piedmont Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C;NSW 1 43.8 1 664 1 13 1 0.4 No Forested/Wetland Rural Residential Agriculture Other (describe) Visible Landuse (%) 50 1 25 25 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) City of Greensboro's North Buffalo Creek WWTP NCO024325 16 Water Quality Parameters Temperature (°C) 16.3 Dissolved Oxygen (mg/L) 8.4 Specific Conductance (pS/cm) 301 pH (s.u.) 7.0 Water Clarity I Very slightly turbid Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 14 5 7 10 4 7 7 5 3 67 Substrate lBoulder, cobble, sand, bedrock Sample Date Sample ID Species Total NCIBI Score NCIBI Rating 05/13/13 2013-19 14 38 Fair 06/23/03 2003-29 10 28 Poor 04/07/98 98-10 6 32 Poor 05/10/94 94-17 5 24 Poor Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Redbreast Sunfish and Bluehead Chub (n=38 (29%) and 35 (27%), respectively. Exotic Species Green Sunfish and Red Shiner (n=3 and 1, respectively) Gained -- Satinfin Shiner (n=7), Whitefin Shiner, Red Shiner, Redfin Pickerel, and Tessellated Darter (n=8) first collections ever; Red Shiner. Lost -- White Sucker. Unless otherwise noted, all species gained or lost were represented by 1 fish/species. Watershed -- drains the northern portion of the City of Greensboro metropolitan area in central Guilford County; site is — 8.5 miles downstream from the City of Greensboro's North Buffalo Creek WWTP and — 02. mile upstream from the creek's confluence with South Buffalo Creek; tributary to Buffalo Creek and ultimately the Haw River. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 17% forest, 7% cultivation, and 73% developed; since 1992 forested land has decreased from 25% to 17% and developed land has increased from 67% to 74%. Habitat -- moderately high quality instream and riparian habitats; two riffles; runs; boulder and bedrock outcrops; side snags and undercuts; hydrologically flashy due to its developed watershed. Water Quality -- greatest specific conductance of any fish community site in 2013 resulting from upstream WWTP and stormwater runoff. 2013 -- a 10 point increase in the NCIBI score and a 1 rating class improvement were due to the first time darters were ever collected at this site, a lower percentage of tolerant fish, and a more balanced trophic structure than in years past. 2004-2013 -- 16 species known from the site, including 3 nonindigenous species; dominant species are Redbreast Sunfish (33%) and Bluehead Chub (26%); no intolerant species have ever been collected at this site; consistently low number of fish collected, but the number of fish has increased 122% between 1998 and 2013 (59 vs. 131); lower than expected species diversity for a stream of its size, but species diversity has been gradually increasing since 1994. Recommendation -- the WWTP is to be decommissioned in late 2015 with the effluent to be diverted to the T. Z. Osborne POTW (NC0047384) for treatment; fish community monitoring should be continued in 2018 to determine if there is any recovery in the community following removal of the discharge. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification POKEBERRY CR I SR 1711 104/17/131 BF109 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CHATHAM 4 1 03030002 1 35.77403511 -79.1200126 16-37 Carolina Slate Belt Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-IV, NSW 11.5 1 321 1 7 1 0.4 1 Yes Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 100 0 0 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q„ MGD) None I --- Water Quality Parameters Temperature (°C) 19.4 Dissolved Oxygen (mg/L) 9.6 Specific Conductance (pS/cm) 99 pH (s.u.) 6.9 Water Clarity I Clear Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 18 10 14 8 6 6 10 5 5 87 Substrate lCobble, gravel, boulder, sand Sample Date SamDle ID Species Total NCIBI Score NCIBI Ratinq 04/17/13 2013-03 19 48 Good 04/15/09 2009-12 24 56 Excellent 07/19/04 2004-130 21 48 Good Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Redbreast Sunfish (n=53, 27%) Exotic Species Green Sunfish and White Sucker, n= 3 and 1, respectively) Gained -- Bowfin, Golden Shiner, and White Sucker first collections ever; and Largemouth Bass. Lost -- Rosyside Dace (n=5), Whitefin Shiner (n=10), Spottail Shiner (n=4), Sandbar Shiner, Creek Chubsucker (n=19), Notchlip Redhorse, Snail Bullhead, Yellow Bullhead, Eastern Mosquitofish, and Piedmont Darter. Unless otherwise noted, all species gained or lost were represented by 1 or 2 fish/species. Watershed -- drains northeastern Chatham County west of Jordan Reservoir; no municipalities or NPDES dischargers within the watershed; tributary to the Haw River; site is — 1.1 miles above the creek's confluence with the river. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories > 5% are reported) -- 75% forest, 13% cultivation, and 7% developed; since 1992 forested land has decreased from 89% to 75% and developed land has increased from <1 % to 7%. Habitats --atypical high -quality Carolina Slate Belt -type stream with very shallow and wide riffles; pools, very slick periphyton-covered rocks, root mats, and snags; Russian Olive abundant in the riparian zones; low flow. Water Quality -- specific conductance has ranged from 84 pS/cm in 2009 to 110 pS/cm in 2004. 2013 -- community was still affected by the late 2012-early 2013 drought; number of fish collected has greatly declined from 722 to 524 to 195 since 2004 (a 73% decrease) primarily due to population declines in Bluehead Chub and Margined Madtom; very low percentage of species with multiple age classes which is often seen in communities recovering from sustained and frequent droughts followed by very high flow effects; 8 point decline in NCIBI score and 1 rating class was due to fewer species, loss of a species of darter, loss of intolerant species, and loss of age classes (9 of 19 species represented by only only 1 or 2 fish/species). 2004- 2013 -- extremely diverse community for a stream of its size which is related to site's proximity to the river with species migrating to and from the river; 32 species known from the site including 11 species of cyprinids, 2 species of darters, and 3 nonindigenous species, but these nonindigenous species comprise only 2% of all the fish ever collected; dominant species is the Bluehead Chub (31 %); community is affected more so by persistent low flows and droughts as are many Carolina Slate Belt streams than by changes in water quality. Recommendation -- based upon all the data collected, there has been no long-term change in water quality in the Pokeberry Creek watershed; continued basinwide assessment of this regional reference site in 2018 to document any potential impacts from increased development in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification POLECAT CR SR 2114 105/14/131 BF53 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion RANDOLPH 9 1 03030003 1 35.8798941 -79.7703185 17-11-(1)b Southern Outer Piedmont Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-III 129.3 1 655 1 7 1 0.4 1 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 1 25 0 75 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None I --- I --- Water Quality Parameters Temperature (°C) 12.0 Dissolved Oxygen (mg/L) 8.8 Specific Conductance (pS/cm) 119 pH (s.u.) 6.3 Water Clarity I Slightly turbid -turbid Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 8 3 1 8 1 6 9 1 3 45 Substrate Iclay, sand, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Rating 05/14/13 2013-21 14 46 Good 06/04/09 2009-55 18 60 Excellent 06/24/03 2003-33 16 46 Good Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Bluegill (n=83, 39%) Exotic Species Green Sunfish (n=1) Gained -- Warmouth (n=2) first collection ever. Lost -- Notchlip Redhorse (n=35), Flat Bullhead (n=1), Pumpkinseed (n=1), Redear Sunfish (n=3), sunfish hybrid (n=1), Black Crappie (n=2), and Piedmont Darter (n=2). Watershed -- drains southern Guilford and north -central Randolph counties; no municipalities within the watershed; two small permitted dischargers in the headwaters (NC0055913 and NC0023299, Total Qw = 0.10 MGD); tributary to the Deep River, impounded — 5 miles downstream by the Camp Nawaka Lake Dam. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 47% forest, 34% cultivation, and 13% developed; since 1992 forested land has decreased from 62% to 47% and developed land has increased from 8% to 13%. Habitats -- good root snags; large deadfalls; deeply entrenched with severely eroding banks; sticks in the current functioning as riffles; riparian zones are hay fields. Water Quality -- specific conductance has ranged from 109 pS/cm in 2003 to 140 pS/cm in 2009. 2013 -- a 14 point decline in NCIBI score and 1 rating class decline (from a very high Excellent to a very low Good) resulted from the number of fish declining by 36% between 2009 and 2013 (334 vs. 213), the loss of several species including Notchlip Redhorse, piscivorous Black Crappie (probably from the downstream reservoir), and the intolerant Piedmont Darter. 2003-2013 -- 23 species known from the site, including 7 species of sunfish, 3 species of suckers, and 3 nonindigenous species; dominant species is the Bluegill (30%); maximum NCIBI score and rating in 2009 were not expected given the low quality instream habitats; metric values in 2013 were more in -line with those calculated in 2003; since 2003 the tolerant fish and trophic metrics have been stable (Percent Tolerant Fish 19%-23%, Omnivores+Herbivores 10%%-18%, and Insectivores 76%%-88%). Recommendation -- based upon all the data collected, there seems to have been a substantial decline in water quality in the Polecat Creek watershed (14 point decline in NCIBI score and 1 rating class change), however, the fish community is also affected by the downstream impoundment which limits recolonization by stream fauna; continued basinwide assessment of this site in 2018 to document any potential impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification REEDY FK I SR 2728 109/05/131 BF65 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion GUILFORD 2 1 03030002 1 36.1795781 -79.647313 16-11-(9)a2 Southern Outer Piedmont Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C;NSW 127 1 648 1 10 0.4 1 No Forested/Wetland Rural Residential Agriculture Other (describe) Visible Landuse (%) 25 1 50 25 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q,, MGD) None I --- Water Quality Parameters Temperature (°C) 21.5 Dissolved Oxygen (mg/L) 6.8 Specific Conductance (pS/cm) 108 pH (s.u.) 7.0 Water Clarity I Clear -slightly tannic Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 14 8 7 6 5 6 8 5 4 68 Substrate JCobble, boulder, bedrock, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 09/05/13 2013-53 13 48 Good 06/25/03 2003-35 15 52 Good 10/12/98 98-84 22 52 Good 04/07/98 98-11 16 48 Good 11 /03/93 93-39 1 15 46 Good Bluehead Chub and Sandbar Shiner Most Abundant Species, 2013 (n=128 (24%) and 113 (21 %), Exotic Species Green Sunfish (n=2) respectively). Species Change Since Last Cycle Gained -- Speckled Killifish (n=25). Lost -- Comely Shiner (n=2), Redfin Pickerel (n=2), Warmouth (n=1), and sunfish hybrid (n=1). uara Watershed -- drains northwest Guilford and a very small portion of northeastern Forsyth counties, including the northern portion of the City of Greensboro metropolitan area; creek is impounded upstream by four major reservoirs and one small mill dam; one small NPDES dischargers (NC0022691, Qw 0.082 MGD) located —6.8 miles upstream; large tributary to the Haw River. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.htmi, only landuse categories >_ 5% are reported) -- 39% forest, 21 % cultivation, and 31 % developed; since 1992 forested land has decreased from 54% to 39% and developed land has increased from 15% to 31 %. Habitat -- site is within the Southern Outer Piedmont, but instream habitats are similar to those in Carolina Slate Belt -type streams; heavy diatom, Fissidens and Podostemum growths in the riffles; riffles at the bridge and at the end of the reach; one long shallow run/pool at low flow; shallow side snags and undercuts; open canopy at the bridge; evidence of persistent and extremely high flows in the spring and summer 2013. Water Quality -- specific conductance relatively stable, ranging from 80 pS/cm in 1998 to 113 pS/cm in 1993. 2013 -- slight decline in NCIBI score due to loss of one species of sunfish and piscivores; total species diversity and diversity of suckers were lower than expected compared with those at reference sites. 2004-2013 -- low diversity for a stream of its size which is most likely related to upstream impoundments and lower water quality downstream below the fork's confluence with Buffalo Creek; only 24 species known from the site, including 3 species of darters, 1 intolerant species, and 3 nonindigenous species; number of species has declined by 41 % since 1998; dominant species are Sandbar Shiner and Bluehead Chub (18% each); Creek Chubsucker has never been collected at this site and suckers have been absent since 1998; trophic metrics are stable since 1998 (23%-27% Omnivores+Herbivores and 73%-77% Insectivores); has consistently been rated Good since 1993. Recommendation -- based upon all the data collected, there has been no change in water quality in the Reedy Creek watershed; continued basinwide assessment in 2018 to document any impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification RICHLAND CR I SR 1154 105/13/131 BF31 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion GUILFORD 8 1 03030003 1 35.941151 -79.932013 17-7-(4) Southern Outer Piedmont Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site W S-IV; CA:* 12.4 1 703 1 6 0.3 1 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 90 0 0 10 (K-V Line) Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q„ MGD) None I --- Water Quality Parameters Temperature (°C) 16.8 Dissolved Oxygen (mg/L) 9.9 Specific Conductance (pS/cm) 192 pH (s.u.) 7.1 Water Clarity I Slightly turbid Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 12 3 2 6 3 6 9 4 5 55 Substrate Isand, cobble along the right bank Sample Date SamDle ID Species Total NCIBI Score NCIBI Ratinq 05/13/13 2013-20 14 40 Good -Fair 04/22/03 2003-06 9 36 Fair 04/22/98 98-19 9 26 Poor Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Spottail Shiner (n=138, 32%) Exotic Species Green Sunfish (n=8) Gained -- Bluehead Chub (n=16), Spottail Shiner (n=138), Swallowtail Shiner (n=77), Sandbar Shiner (n=20), Satinfin Shiner (n=16), Snail Bullhead (n=2), and Tessellated Darter (n=7) first collections ever. Lost -- Rosyside Dace (n=15) and Golden Shiner (n=1). Watershed -- drains southwest Guilford County, including the eastern portion of the City of High Point and the US 29/70 corridor; one water treatment facility (NC0081256, Qw = unlimited) located on an unnamed tributary in the creek's headwaters; tributary to Randleman Reservoir (Deep River); site is — 2.1 miles upstream from the creek's confluence with the reservoir. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north carolina.html, only landuse categories >_ 5% are reported) -- 15% forest, 5% cultivation, and 78% developed; since 1992 forested land has decreased from 26% to 15% and developed land has increased from 65 %to 78%. Habitat -- shallow, sandy runs and side snags; coarse woody debris; primarily stick riffles with one gravel/cobble riffle; well -canopied with wide riparian zones; carries a heavy sediment (sand) and urban debris (tires and plastics) load; hydrologically flashy due to its developed watershed. Water Quality -- specific conductance, elevated from urban stormwater and nonpoint run-off, has ranged from 158 pS/cm to 192 pS/cm. 2013 -- the nine species collected for the 1 st time at this site accounted for 63% of all the fish collected (276 out of 438); species such as Spottail Shiner and Satinfin Shiner are often reservoir -inhabitants, thus they might have migrated from the upper end of the reservoir back into the creek, others such as Tessellated Darter, Bluehead Chub, Swallowtail Shiner, and Sandbar Shiner must have re -populated the creek since 2003 prior to completion of the dam; increases in total species diversity, diversity of darters, and a lower percentage of tolerant fish accounted for the slight increase in the NCIBI score and rating. 1998-2013 -- 17 species known from the site, including just 1 nonindigenous species (Green Sunfish); intolerant species are absent; dominant species is the tolerant Redbreast Sunfish (35%), but its dominance has steadily decreased from 62% to 37% to 21 % since 1998; the Percentage of Tolerant fish decreased from 67% in 1998 and 2003 to 31% in 2013; steady improvement in the NCIBI scores and ratings since 1998. Recommendation -- based upon all the data collected, there has been an unexpected improvement in water quality in the Richland Creek watershed; continued basinwide assessment in 2018 to document if the rating is able to be maintained in an increasingly developed watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification ROCKY R SR 1300 104/17/131 BF33 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CHATHAM 12 1 03030003 1 35.807006-79.52769 17-43-(1)a Carolina Slate Belt Stream Classification Drainage Area (mi) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-III 7.4 617 6 0.3 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 50 0 50 0 Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q., MGD) None I --- I --- Water Quality Parameters Temperature (°C) 16.3 Dissolved Oxygen (mg/L) 8.0 Specific Conductance (pS/cm) 104 pH (s.u.) 6.3 Water Clarity I Clear, stained Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 12 5 10 10 4 6 8 3 5 68 Substrate lCobble, gravel, boulder, bedrock, sand Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 04/17/13 2013-01 12 44 Good -Fair 04/15/09 2009-10 17 50 Good 05/06/03 2003-18 15 40 Good -Fair 05/04/98 98-26 15 44 Good -Fair Redbreast Sunfish and Bluehead Chub Most Abundant Species, 2013 (n=138 (33%) and 112 (27%), Exotic Species Green Sunfish (n=16) respectively) Species Change Since Last Cycle Gained -- none. Lost -- Golden Shiner (n=1), Whitemouth Shiner (n=1), Highfin Shiner (n=11), Pumpkinseed Sunfish (n=5), Redear Sunfish (n=30, and sunfish hybrid (n=2). Data Analysis Watershed -- headwaters originate in the Town of Liberty; drains northeast Randolph and northwestern Chatham counties; no NPDES dischargers or municipalities within the watershed; site is — 4.3 miles upstream from the backwaters of the Slier City water supply reservoir. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories > 5% are reported) -- 33% forest, 44% cultivation, and 17% developed; since 1992 forested land has decreased from 47% to 33% and developed land has increased from 11 % to 17%. Habitat -- situated in the Carolina Slate Belt although the substrate includes an abundance of gravel and sand; periphyton abundant atop the substrate; riffles only at the beginning of the reach; runs and bedrock pools; some Justicia; open woods and canopy in places; pasture along the left bank; cattle with access, but no evidence of them using the pasture or entering the stream, cattle exclusion barrier across the stream; low flow. Water Quality -- specific conductance relatively stable, ranging from 81 pS/cm in 1998 to 104 pS/cm in 2013. 2013 -- an abundant community, one of the few sites where the number of fish increased between 2009 and 2013 (321 vs. 420); 89% more fish were collected in 2013 than in 2003 (222 vs. 420); but with fewer species being collected and a greater percentage of them tolerant (e.g., Redbreast Sunfish, Eastern Mosquitofish, and Green Sunfish), the NCIBI score and rating declined. 1998-2013 - diverse stream for its size, 19 species known from the site, intolerant species are absent; from 2003 to 2103 the number and percent abundance of Margined Madtom, Redbreast Sunfish, and Tessellated Darter have steadily increased; dominant species are Bluehead Chub (37%) and Redbreast Sunfish (22%); Percentage of Tolerant Fish has increased since 2003 from 15% to 35% to 44%; 3 of the 4 times assessed, the community has rated Good -Fair. Recommendation -- based upon all the data collected, community is likely sensitive to flow and drought regimes; no long-term change in water quality in the Rocky River watershed is suggested by the biological data; continued basinwide assessment of this site in 2018 to document any potential impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification S PR STINKING QUARTER CR SR 1117 104/24/131 BF28 I Excellent County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion ALAMANCE 3 03030002 1 5,9892801-79.496674 16-19-8-2-(2) Southern Outer Piedmont Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C;NSW 1 33.6 1 500 1 9 1 0.4 No Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 100 0 0 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None I --- I --- Water Quality Parameters Temperature (°C) R6.8 Dissolved Oxygen (mg/L)Specific Conductance (pS/cm)pH (s.u.) Water Clarity I Clear, slightly stained Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 12 4 3 4 4 6 10 5 5 58 Substrate Sand, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 04/24/13 2013-14 22 56 Excellent 06/04/09 2009-53 22 52 Good 04/24/03 2003-11 20 54 Excellent Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Crescent Shiner, Redear Sunfish, Green Redbreast Sunfish (n=78, 16%) Exotic Species Sunfish, White Sucker (n=55, 9, 2, and 1, respectively) Gained -- White Shiner (n=19) and Redear Sunfish (n=9) first collections ever; Margined Madtom and Piedmont Darter. Lost -- Brassy Jumprock, Snail Bullhead, Largemouth Bass, and Black Crappie. Unless otherwise noted, all species gained or lost were represented by 1 fish/species. -- drains the extreme southeastern corner of Guilford, northeastern Randolph, and southwestern Alamance counties; no NPDES dischargers or municipalities within the watershed; tributary to Big Alamance Creek. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 48% forest, 40% cultivation, and 6% developed; since 1992 forested land has decreased from 65% to 48% and developed land has increased from < 1 % to 6%. Habitats -- very shallow runs and pools with side snags, gravel and sand bars, and stick riffles; coarse woody debris and heavy diatom growths; stream channel is entrenched; cattle are fenced out on the right side; good riparian zones along both banks; low flow. Water Quality -- specific conductance has ranged from 84 pS/cm in 2003 to 127 pS/cm in 2013. 2013 -- most species (n=22) collected from any site in the basin; only metric not scoring the maximum score (5) was the Percentage of Piscivores. 2003-2013 -- a stable and abundant diverse community; 28 species known from the site, including 2 darters, 1 intolerant species, and 5 nonindigenous species; dominant species are the Swallowtail Shiner (17%) and Redbreast Sunfish (14%); number of fish collected is very stable, ranging from 442 to 484; Percentage of Tolerant Fish stable (25%-26%); and trophic metrics are stable (10%-19% Omnivores+Herbivores and 81 %-89% Insectivores);community does not seem to be affected by extreme flows as much as does the community in the adjacent North Prong Stinking Quarter Creek watershed which has similar landuse characteristics but a smaller drainage area (20.8 mi2); did not rate Excellent in 2009 because the intolerant Piedmont Darter was represented only by young -of -year rather than by juveniles or adults and was not included in the calculations; NCIBI ratings have ranged from high Good to Excellent. Recommendation -- based upon all the data collected, there has been no change in water quality in the South Prong Stinking Quarter Creek watershed; continued basinwide assessment in 2018 to document any impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification SANDY CR I SR 2481 105/14/131 BF62 I Excellent County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion RANDOLPH 1 9 1 03030003 1 35.785251 -79.665604 17-16-(1)a Carolina Slate Belt Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site W S-I I I; CA 1 45.1 1 503 1 16 0.4 1 No ForestediWetland Urban Agriculture Other (describe) Visible Landuse (%) 100 0 0 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q„ MGD) None I --- Water Quality Parameters Temperature (°C) 13.9 Dissolved Oxygen (mg/L) 9.3 Specific Conductance (pS/cm) 117 pH (s.u.) 7.0 Water Clarity I Clear Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 18 10 16 10 7 7 8 4 4 89 Substrate lCobble, boulder, bedrock, gravel Sample Date SamDle ID Species Total NCIBI Score NCIBI Ratinq 05/14/13 2013-22 15 56 Excellent 06/24/03 2003-31 15 52 Good 06/01/99 99-39 18 56 Excellent 05/04/98 98-25 19 60 Excellent 03/22/94 94-04 1 15 52 Good Most Abundant Species, 2013 IBluegill (n=102, 30%) 1 Exotic Species lGreen Sunfish (n=14) Species Change Since Last Cycle Gained -- Golden Shiner (n=1) and Brown Bullhead (n=13) first collections ever; and Creek Chub (n=2). Lost - Redlip Shiner (n=4), Pumpkinseed (n=1), Redear Sunfish (n=3), and sunfish hybrid (n=9). Data Analysis Watershed -- drains northeastern Randolph County; one small permitted discharger (NC0040941, Qw = 0.009 MGD) located on an unnamed headwater tributary; site is located — 1.9 miles downstream from Kidds Mill Dam and — 0.9 miles upstream from Sandy Creek Reservoir; tributary to Deep River. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 49% forest, 38% cultivation, and 7% developed; since 1992 forested land has decreased from 64% to 49% and developed land has increased from 2% to 7%. Habitat -- high quality Carolina Slate Belt -type instream habitats; boulder and snag pools; frequent riffles; open canopy at low-water bridge; abundant Fissidens and Podostemum in the riffles; slick, periphyton covered rocks; breaks in the riparian zone at the bridge allowing sediment to runoff into the creek. Water Quality -- specific conductance increased between 2003 to 2013 (from 79 pS/cm to 117 pS/cm), but variable over the time period ranging from 70 pS/cm in 1994 to 117 pS/cm in 2013. 2013 -- one of the few sites were there was an increase in the number of fish collected between monitoring cycles (342 vs 179); a 4 point increase in the NCIBI score and 1 rating class increase due to more fish being collected and a more balanced trophic structure. 2004-2013 -- 27 species known from this stream, including 3 species of suckers, 2 species of darters, 1 intolerant species, and 1 nonindigenous species; dominant species is Bluehead Chub (31 %); recolonization following droughts or prolonged low flows is affected by limited downstream source populations in tributary streams because of the downstream reservoir; Redlip Shiner, a nonindigenous species in the basin, was 23% of all fish collected in 1999, in 2013 it was absent; Percentage of Tolerant fish stable ranging from 7%-16%, trophic metrics relatively stable (Omnivores+Herbivores 23%-38% and Insectivores 55%-71 %); NCIBI scores and ratings have ranged from a high Good to a high Excellent. Recommendation -- based upon all the data collected, community is responsive to flow and drought regimes; no long-term change in water quality in the Sandy Creek watershed; continued basinwide assessment of this site in 2018 to document any potential impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification S BUFFALO CR US 70 104/23/131 BF73 I Poor County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion GUILFORD 2 03030002 1 36,089368-79.688088 16-11-14-2b Southern Outer Piedmont Stream Classification Drainage Area (mi2) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C;NSW 1 39.5 685 9 0.3 No Forested/Wetland Urban Visible Landuse (%) 40 10 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) Agriculture Other (describe) 0 50 (Commercial and Industrial) NPDES Number Volume (Q., MGD) None I --- Water Quality Parameters Temperature (°C) M73 Dissolved Oxygen (mg/L) Specific Conductance (pS/cm) pH (s.u.) Water Clarity IClear Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 4 7 3 0 4 2 5 5 5 5 40 Substrate Sand, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Rating 04/23/13 2013-12 7 24 Poor 06/23/03 2003-28 7 26 Poor 04/21 /99 99-22 7 22 Poor 04/07/98 98-08 6 26 Poor 05/10/94 94-15 10 30 Poor Most Abundant Species, 2013 jEastern Mosquitofish (n=47, 40%) 1 Exotic Species lGreen Sunfish (n=4) Species Change Since Last Cycle Gained -- Flat Bullhead (n=1). Lost -- Red Shiner (n=4). Data Analysis Watershed -- drains eastern and southeastern portions of the City of Greensboro metropolitan area, including the 1-40/1-85 corridor; one very small (NC0088773; Qw = 0.001 MGD) groundwater remediation facility located in the creek's headwaters. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 12% forest, 5% cultivation, and 81 % developed; since 1992 forested land has decreased from 23% to 12% and developed land has increased from 68% to 81 %. Habitat -- an urban stream with elevated conductivity, tires, urban debris, lack of instream habitats, and hydrologically flashy; one of the lowest habitat scores of any fish community site in the basin in 2013; riffles absent, bank erosion, shallow sandy runs, some side snags; forested and wide riparian zones. Water Quality -- 2"d greatest specific conductance measurements of any fish community site in the basin in 2013 resulting from stormwater runoff and has ranged from 197-266 µS/cm. 2013 -- fewest species (n=7) and lowest NCIBI score (24) of any site in the basin; number of fish declined by 60% between 2003 and 2013 (293 vs. 118); suckers and intolerant species absent; very skewed trophic structure with only 1 % Omnivores+Herbivores. 1994-2013 -- the least diverse fish community site in the basin, only 13 species are known from the site, including 2 nonindigenous species; 4 of the 9 species (Golden Shiner, Snail Bullhead, Pumpkinseed, and Warmouth) have not been collected since 1994; dominant species are the tolerant Eastern Mosquitofish and Redbreast Sunfish; fish community has consistently been rated Poor; the number of the nonindigenous and tolerant Red Shiner has declined from 116 in 1999 to 0 in 2013; no suckers, intolerant species, or piscivores have ever been collected from this site; very skewed trophic structure dominated by Insectivores (97%-100%). Recommendation -- based upon all the water quality, fish community, and habitat quality data collected, this stream has consistently been impaired; future monitoring can be reduced to once every 10 years or eliminated. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification STONY CR SR 1104 104/22/131 BF26 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CASWELL 2 03030002 36,2573257 79.4469673 16-14-(1)a Southern Outer Piedmont Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-II;HQW,NSW 1 12.5 1 596 7 0.4 Yes Forested/Wetland Urban Agriculture Other (describe) Visible Landuse (%) 1 100 0 0 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None I --- I --- Water Quality Parameters Temperature (°C) R7.0 Dissolved Oxygen (mg/L) Specific Conductance (pS/cm) pH (s.u.) Water Clarity I Slightly turbid, green Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 13 3 3 10 5 7 9 5 5 65 Substrate Boulder, gravel, sand Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 04/22/13 2013-08 17 46 Good 06/03/09 2009-51 15 48 Good 04/21 /03 2003-02 14 44 Good -Fair 05/19/94 94-21 18 54 Excellent Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Crescent Shiner (n=50, 24%) Exotic Species Crescent Shiner and Green Sunfish (n=50, and 1, respectively) Gained -- Flat Bullhead (n=1) first collection ever; Rosyside Dace (n=1), Snail Bullhead (n=1), and Largemouth Bass (n=1). Lost -- Warmouth (n=2) and Bluegill (n=11). -- drains southwestern Caswell County; no municipalities or NPDES dischargers within the watershed; impounded downstream by Lake Burlington. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.htmi, only landuse categories >_ 5% are reported) -- 53% forest, 35% cultivation, and 5% developed; since 1992 forested land has decreased from 69 to 53%, cultivated land has increased from 29% to 35%, and developed land has increased from 2% to 5%. Habitats -- channel filled with sediment in places; mid -channel runs, pools, and side boulders; infrequent stick and gravel riffles; snags and undercuts, banks vegetated, right riparian zone intact but logged circa 2010; angular rocks covered with periphyton; low flow. Water Quality -- specific conductance has ranged from 84 pS/cm in 2003 to 120 pS/cm in 2009. 2013 -- number of fish declined by 66% between 2009 and 2013 (629 vs. 208); slight changed in the NCIBI score but not the rating due to fewer fish and species of sunfish collected in 2013 than in 2009 but that was counteracted by an overall increase in species diversity and a slight increase in the Percentage of Piscivores. 1994-2013 -- 22 species are known from the site, including 3 nonindigenous species, but no intolerant species; dominant species is the Crescent Shiner (26%); trophic metrics stable (10%-25% Omnivores+Herbivores and 74%-89% Insectivores). Recommendation -- based upon all the data collected, there has been no long-term change in water quality in the Stony Creek watershed; continued basinwide assessment of this regional reference site in 2018 to document any potential impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification TERRELLS CR NC 87 105/16/131 BF9 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CHATHAM 4 03030002 1 35.8219161 -79.255539 1 16-31-(2.5) Carolina Slate Belt Stream Classification Drainage Area (mi) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site WS-IV;NSW 21.0 1 420 1 7 0.4 1 No Forested[Wetland Urban Agriculture Other (describe) Visible Landuse (%) 100 1 0 0 0 Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Qw, MGD) None Water Quality Parameters Temperature (°C) 16.9 Dissolved Oxygen (mg/L) 8.2 Specific Conductance (pS/cm) 101 pH (s.u.) 6.6 Water Clarity I Clear Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) Sample Date 5 18 12 10 10 6 7 10 5 5 88 Sample ID Substrate lCobble, boulder, bedrock, gravel, sand Species Total NCIBI Score NCIBI Rating 05/16/13 2013-28 12 48 Good 06/12/08 2008-59 13 48 Good 05/15/08 2008-38 11 44 Good -Fair 04/17/08 2008-18 13 46 Good 03/13/08 2008-02 10 42 Good -Fair 04/24/03 2003-12 16 56 Excellent 04/21 /98 98-15 17 52 Good 04/19/94 94-10 14 50 Good Most Abundant Species, 2013 Highfin Shiner (n=100, 43%) Exotic Species Green Sunfish and Redear Sunfish (n=11 and 1, respectively) Species Change Since Last Cycle Gained -- sunfish hybrid first collection ever; Redear Sunfish. Lost -- Snail Bullhead and Chain Pickerel. All species gained or lost were represented by 1 fish/species. Data Analysis Watershed -- drains north -central Chatham County, no NPDES dischargers or municipalities within the watershed; tributary to the Haw River, site is — 3.1 miles above the creek's confluence with the river and —2.2 miles upstream from the Baldwin Mill Pond Dam, built circa 1820. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.html, only landuse categories >_ 5% are reported) -- 56% forest, 34% cultivation, and 5% grassland/herbaceous; since 1992 forested land has decreased from 67% to 56% and developed land has increased from < 1 % to 3%. Habitats -- typical high quality Carolina Slate Belt -type habitats -- shallow and frequent riffles and runs; Fissidens (river moss); boulder pools, snags, root mats, and undercuts; slick rocks; wide forested riparian zones; no change in habitats since last cycle. Water Quality -- specific conductance relatively stable since 1994 ranging from 73 pS/cm in 2003 to 101 pS/cm in 2013. 2013 -- as in cycles past, lower than expected total species diversity; intolerant species and piscivores were absent. 1994-2013 -- 23 species are known from this site including 6 species of sunfish, 3 species of darters (including the Carolina Darter, Etheostoma collis, a state Special Concern Species which has been collected here six times since 2003), 1 intolerant species, and 2 nonindigenous species; dominant species are Highfin Shiner (27%), Bluehead Chub (13%), and Whitemouth Shiner (12%); several species have not been collected since 1998 or 2003 (e.g., Creek Chub, Brassy Jumprock, Yellow Bullhead, Flat Bullhead, Pumpkinseed, and Piedmont Darter); this site has rated Good 5 of the 8 times it has been sampled; affected more so by low flows and droughts as are many Carolina Slate Belt streams. However, it is also affected by the downstream impoundment which limits recolonization by stream fauna. Drought recovery studies conducted in 2008 were summarized in BAU Memorandum F- 20090122. Recommendation -- based upon all the data collected, there has been no change in water quality in the Terrells Creek watershed; continued basinwide assessment in 2018 to document any impacts from changing landuse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification TICK CR I SR 2170 105/15/131 BF136 I Good -Fair County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion CHATHAM 12 1 03030003 1 35.673461 -79.365912 17-43-13b Carolina Slate Belt Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C 1 19.3 1 419 1 6 1 0.4 No ForestediWetland Urban Agriculture Other (describe) Visible Landuse (%) 50 1 0 50 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q„ MGD) None I --- Water Quality Parameters Temperature (°C) 14.6 Dissolved Oxygen (mg/L) 7.5 Specific Conductance (pS/cm) 139 pH (s.u.) 6.6 Water Clarity I Clear, easily silted Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 14 8 7 8 5 6 9 3 4 69 Substrate JCobble, sand, silt, clay, gravel Sample Date Sample ID Species Total NCIBI Score NCIBI Ratinq 05/15/13 2013-25 15 42 Good -Fair 06/11/09 2009-65 16 52 Good 06/13/203 2003-24 @ US 421 15 38 Fair 04/19/94 94-09 @ US 421 17 56 Excellent Most Abundant Species, 2013 JGreen Sunfish (n=53, 2901.) Exotic Species JGreen Sunfish (n=53) Species Change Since Last Cycle Gained -- Margined Madtom (n=1). Lost -- sunfish hybrid (n=2), Bluegill (n=3), and Piedmont Darter (n=1). Data Analysis Watershed -- drains southwest Chatham County; no municipalities or NPDES dischargers within the watershed; in 2009 the site was moved 2.8 miles downstream from the US 421 site to assess more of the watershed (20.3 vs.15.5 mi2); no large or named streams join the creek between the two sites; tributary to the Rocky River, site is — 2 miles upstream of the creek's confluence with the river and at the last possible access to the creek at a bridge crossing. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.htm], only landuse categories >_ 5% are reported) -- 57% forest, 30% cultivation, and 5% developed; since 1992 forested land has decreased from 71 % to 57% and developed land has increased from 1 % to 5%. Habitat -- Carolina Slate Belt -type stream with large deadfall across the upper one-third of the reach; habitats same as in previous cycle; shallow; exposed cobble bars; undercuts and snags; Justicia and Podostemum in the riffles; long pools with bank snags; low flow with many dry riffles; cattle fenced out along both banks. Water Quality -- specific conductance variable, ranging from 108 pS/cm in 1994 to 121 pS/cm, 160 pS/cm, and 139 pS/cm during the past three cycles. 2013 -- 10 point decline in NCIBI score and 1 rating class decline resulted from the loss of the intolerant Piedmont Darter and an abundance of tolerant Green Sunfish, Eastern Mosquitofish, Redbreast Sunfish, and Golden Shiner (50% of all the fish collected). 1994-2013 -- 22 species are known from the two sites, including 2 species of darters, 1 intolerant species, and 2 nonindigenous species; dominant species are the Highfin Shiner and Green Sunfish (16% each); nonindigenous Green Sunfish, a species that can become abundant in low flow Carolina Slate Belt streams, has displaced the native Redbreast Sunfish; data suggest the decline in rating may be affected affected more so by low flows and droughts as are many Carolina Slate Belt streams than by changes in water quality. Recommendation -- based upon all the data collected, there may be a decline in water quality in the Tick Creek watershed (slight increases in specific conductance, 10 point decline in NCIBI score, and 1 rating class change), however, the community may also be affected by flow extremes; continued basinwide assessment of this site in 2018 to document any potential impacts from changing Ianduse in the watershed. FISH COMMUNITY SAMPLE Waterbody Location Date Station ID Bioclassification VARNALS CR I SR 2116 104/18/131 BF119 I Good County Subbasin 8 digit HUC Latitude Longitude AU Number Level IV Ecoregion ALAMANCE 2 1 03030002 1 35.98720971 -79.3587846 16-21b Carolina Slate Belt Stream Classification Drainage Area (mil) Elevation (ft.) Stream Width (m) Average Depth (m) Reference Site C;NSW 1 11.6 1 450 1 7 1 0.4 No Forested/Wetland Rural Residential Agriculture Other (describe) Visible Landuse (%) 80 1 20 0 0 Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (Q„ MGD) None I --- Water Quality Parameters Temperature (°C) 17.0 Dissolved Oxygen (mg/L) 8.4 Specific Conductance (pS/cm) 89 pH (s.u.) 6.2 Water Clarity I Clear, slightly stained Habitat Assessment Scores (max) Channel Modification (5) Instream Habitat (20) Bottom Substrate (15) Riffle Habitat (16) Pool Variety (10) Erosion (7) Bank Vegetation (7) Light Penetration (10) Left Riparian Score (5) Right Riparian Score (5) Total Habitat Score (100) 5 16 12 14 8 7 5 10 2 5 84 Substrate 113oulder, cobble, gravel, bedrock Sample Date SamDle ID Species Total NCIBI Score NCIBI Ratinq 04/18/13 2013-04 18 48 Good 04/13/09 2009-07 24 54 Excellent 06/09/04 2004-77 18 46 Good Most Abundant Species, 2013 Species Change Since Last Cycle Data Analysis Bluehead Chub (n=96, 23%) Crescent Shiner, Green Sunfish, Rosefin Shiner, Exotic Species and Mountain Redbelly Dace, n = 89, 13, 12, and 1, respectively. Gained -- Mountain Redbelly Dace, Satinfin Shiner, Eastern Mosquitofish, and Warmouth first collections ever; and Flat Bullhead. Lost -- Highfin Shiner, Comely Shiner, Spottail Shiner (n=10), Swallowtail Shiner (n=21), Fathead Minnow (n=4), White Sucker, Redfin Pickerel (young -of -year only), Speckled Killifish, Pumpkinseed (n=5), sunfish hybrid, Black Crappie, and Oriental Weatherfish. Unless otherwise noted, all species gained or lost were represented by 1 or 2 fish/species. -- drains central Alamance County; no NDPES dischargers or municipalities in the watershed; tributary to the Haw River, site is — 0.7 miles above the creek's confluence with the river. Landuse 2006 (from USGS, http://water.usgs.gov/osw/streamstats/north_carolina.htm], only landuse categories >_ 5% are reported) -- 60% forest, 27% cultivation, 6% developed, and 5% grassland/herbaceous; since 1992 forested land has decreased from 72% to 60% and developed land has increased from < 1 % to 6%. Habitat -- a typical Carolina Slate Belt -type stream with high quality riffles and pools, angular rocks covered with slick periphyton; variety of sizes and depths to the pools and riffles; lawn and house along the left bank, forested riparian along the right bank; low flow. Water Quality -- specific conductance relatively stable, ranging from ranging from 70 pS/cm in 2009 to 89 pS/cm in 2013. 2013 -- loss of a species of sucker and a low percentage of piscivores resulted in the decline in the NCIBI score and rating, but still a diverse and abundant community. 2004- 2013 -- very diverse community for a stream of its size which is related to site's proximity to the river with species migrating to and from the river; 31 species known from the site including 12 species of cyprinids, 7 nonindigenous species, but no intolerant species; dominant species is the Bluehead Chub (18%), Crescent Shiner (13%), Margined Madtom (13%), Bluegill (12%), and Green Sunfish (11 %); trophic metrics are stable (19%-25% Omnivores+Herbivores and 75%-81 % Insectivores); affected more so by low flows and droughts as are many Carolina Slate Belt streams than by changes in water quality. Recommendation -- based upon all the data collected, there has been no change in water quality in the Varnals Creek watershed; continued basinwide assessment in 2018 to document any impacts from changing landuse in the watershed.