NCS000575_Fish tissue monitoring results_20230726

(' DUKE
ENERGY
outhit
PROGRESS Keith anagei
Plant General Manager
�V Asheville Combined Cycle Station
FedEx Tracking: 772770924304 �� r0?� Duke Energy Progress
AS VL PL T I 46 Duke Energy Lane
Jul I9 2023 �� Arden,NC 28704
y ,
� �N o: 828-650-0620
File No.: 12520R O t. 828-650-0701
keith.douthit@ duke-energy.com
Brianna Young
NCDEMLR Stormwater Program
1612 Mail Service Center
Raleigh, North Carolina 27699-1612
Subject: NPDES wastewater permit renewal NC0000396 - Fish Tissue Monitoring report
NPDES industrial stormwater permit NCS000575
Dear Ms. Young,
As required per the above referenced industrial stormwater permit, attached please find a copy of
the Fish Tissue Monitoring report submitted with the renewal package for industrial wastewater
permit NC0000396.
Should you have any questions or comments concerning these reports, please contact Teresa
Williams at [phone] or Don Safrit at 919-546-6416.
Certification by Responsible Official:
"I certify, under penalty of law, that this document and all attachments were prepared under my
direction or supervision in accordance with a system designed to assure that qualified personnel
properly gather and evaluate the information submitted. Based on my inquiry of the person or
persons who manage the system, or those persons directly responsible for gathering the
information, the information submitted is, to the best of my knowledge and belief, true, accurate,
and complete. I am aware that there are significant penalties for submitting false information,
including the possibility of fines and imprisonment for knowing violations.".
Sincerely,
,,
Amber Iciano
Acting, Asheville Station Manager
Enclosure

be: Don Safrit
Teresa Williams
Amber Iciano
Kyle Hussey

Asheville Combined-Cycle Station
al �
Fish Tissue Monitoring of the French Broad River
Buncombe County, North Carolina (2019-2022)
NPDES .Permit No. NC0000396
Duke Energy Progress
May, 2023

Table of Contents
Page
1.0 Introduction..................................................................................................................... 1
2.0 Methods............................................................................................................................ 1
2.1 Study Site.................................................................................................................... 1
2.2 Target Species............................................................................................................. 1
2.3 Field Sampling Methods............................................................................................. 2
2.4 Laboratory Process and Analysis................................................................................ 2
3.0 Results and Discussion.................................................................................................... 2
3.1 Arsenic........................................................................................................................ 3
3.2 Mercury...............................................................................................
3.3 Selenium .........................................................................................................
4.0 Summary.......................................................................................................................... 4
5.0 References........................................................................................................................ 4
List of Tables
Table Page
3-1 Numbers and total length ranges (mm) of species of fish collected for trace element
analysis in the three sampling areas of the French Broad River during 2019-2022......... 3
3-2 Means and ranges of trace elements in fish muscle tissue collected from three
sampling locations in the French Broad River during 2019-2022.................................... 4
List of Figures
Figure Page
1 French Broad River trace element monitoring locations.................................................. 5
Appendices
Appendix A: Arsenic,mercury, and selenium concentrations(wet weight) in axial muscle of fish
from the French Broad River 2019-2022.
Appendix B: Baseline mercury and selenium concentrations (wet weight) in axial muscle of fish
from the French Broad River during August and November 2004.

Fish Tissue Monitoring of the French Broad River Near Asheville Combined Cycle Station(2019-2022)
1.0 Introduction
Duke Energy Progress (DEP) owns and operates the Asheville Combined-Cycle Station
(Asheville Station) located on the east side of the French Broad River in Buncombe County,
Arden,North Carolina. As currently required by the Asheville Station's National Pollutant
Discharge Elimination System (NPDES) Permit No.NC0000396 (current through November 30,
2023), Special Condition A. (15):
"The facility shall conduct fish tissue monitoring annually and submit the results with the
NPDES permit renewal application. The objective of this monitoring is to evaluate
potential uptake of pollutants by fish tissue near the ash pond discharge. The parameters
analyzed in fish tissue shall include arsenic, selenium, and mercury. The monitoring shall
be conducted in accordance with the sampling plan approved by the Division. "
Fish tissue monitoring was originally only required for mercury and selenium after Flue Gas
Desulfurization(FGD) operations commenced in December of 2005. Operation of the FGD
system discontinued when the coal fired generation system was decommissioned in 2019. Electric
generation has transitioned away from coal fired generation to natural gas (combustion turbines).
This data report is submitted to provide an overview of the sampling conducted during the permit
cycle and to fulfill the NPDES permit renewal application.
2.0 Methods
2.1 Study Site
Fish were collected from three locations within the French Broad River (Figure 1). These
locations were adjacent to the Asheville Station discharge (Station DI), 6.2 kilometers upstream
of the discharge(Station UP) and 10.8 kilometers downstream of the discharge(Station DN).
2.2 Target Species
Sunfish (Lepomis) and black bass (Micropterus spp.)/rock bass (Ambloplities spp.) were targeted
for collection based on prior sampling experience, likely availability, potential for human
consumption, and general trophic level. An attempt was made to collect ten fish per target species
with the understanding that six would satisfy permit requirements. As recommended by the U.S.
1

Environmental Protection Agency (USEPA) an attempt was made to limit the smallest fish to 75%
of the largest fish total length by species depending on availability (USEPA 2000).
2.3 Field Sampling Methods
Fish were collected using electrofishing procedures specified in the Duke Energy Environmental
Science Field Programs procedures (ESFP-SW-0200, Rev. 0 and ESFP-SW-0600, Rev. 0) which
were approved by the North Carolina Department of Environmental Quality under the DEP
Biological Laboratory Certification (# 006). Only live fish that showed little or no signs of
deterioration were retained for analysis, put in a labeled bag, and placed on ice until frozen.
Ancillary fisheries data including species, total length (mm), and total weight (g) were also
recorded. Fish collected were transferred to a freezer daily and maintained frozen until processing
at the DEP New Hill Trace Element Laboratory. Associated water quality data including water
temperature,dissolved oxygen, and specific conductance were recorded daily at the surface at each
sampling location(available on request).
2.4 Laboratory Processing and Analysis
All fish samples were processed individually and analyzed in the trace element laboratory
according to procedures ESFP-SW-0601, Rev. 0 and ESFP-SW-0602 Rev. 0. Fish were thawed
and skeletal muscle was fileted off using a carbon-steel scalpel. Muscle tissue from each
individual fish was homogenized, weighed to the nearest 0.001 g, and completely dried in a
desiccator. The dried weight was recorded and used to calculate a dry weight to fresh(wet)
weight ratio for that fish. Approximately 0.1 g of dried tissue was analyzed for each fish using X-
ray fluorescence. Quality control was achieved utilizing analytical standards, replicates, and
certified reference materials. Following analysis, residual processed samples were archived and
will be kept for at least two years in the event re-analysis is needed.
3.0 Results and Discussion
During the study period (2019-2022), 209 individuals representing seven species were captured
and analyzed from the French Broad River (Table 3-1). These species included Smallmouth Bass
Micropterus dolomieu, Largemouth Bass M. salmoides, Alabama Bass M. henshalli, Rock Bass
Amboplities rupestris, Redbreast Sunfish Lepomis auritus, Bluegill L. macrochirus, and Redear
Sunfish L. microlophus. A full list of individual fish and associated trace element concentrations
2

(wet weight) is presented in Appendix A. In addition to the length and weight of each fish,the
dry-to-wet weight ratios are presented on individual fish to convert the arsenic, mercury, and
selenium concentrations wet weight values back to dry weight values as desired. The 2004
baseline data(prior to the flue gas desulfurization system installation for the coal-fired plant) are
also presented for comparison purposes (Appendix B).
Table 3-1. Numbers and total length ranges (mm) of species of fish collected for trace
element analysis in the three sampling areas of the French Broad River during 2019-2022.
UP DI DN
Number Range Number Range Number Range
Smallmouth Bass 15 192-377 24 170-473 22 219420
Black bass Largemouth Bass 13 248-488 3 178-212 8 454-245
(Micropterus)l g
Rock bass Alabama Bass 1 230 1 390 -
(Amboplities)
Rock Bass 4 186-225 3 154-220 4 175-212
Redbreast Sunfish 33 161-203 26 125-205 32 145-225
Sunfish Bluegill 3 177-185 8 140-177 5 138-194
(Lepom is)
Redear Sunfish 3 184-208 - 1 170
3.1 Arsenic
All fish collected during 2019-2022 at the three sample locations were well below the USEPA
screening value for recreational anglers of 1.2 µg/g (wet weight) for arsenic (USEPA 2000; Table
3-2).
3.2 Mercury
One of I I I sunfish species collected during 2019-2022 was above the USEPA recreational
screening value of 0.4 µg/g (wet weight) for mercury (USEPA 2000; Table 3-2). Of the 98 black
bass collected 40 were above the 0.4 µg/g mercury screening value. Of these 40 exceedances 16
were from UP, 13 from DI, and 13 from DN.
3.3 Selenium
All fish collected during 2019-2022 at the three sample locations were well below the USEPA
screening value for recreational anglers of 20 µg/g (wet weight) (USEPA 2000; Table 3-2).
3

Table 3-2. Means and ranges of trace elements in fish muscle tissue collected from three
sampling locations in the French Broad River during 2019-2022. All values are in µg/g wet
weight, and the USEPA screening values are shown next to each element. When
concentrations were less than the reporting limit, the reporting limit value was used for
analysis.
Arsenic(1.2) Selenium (20) Mercury(0.4)
Mean Range Mean Range Mean Range
UP Black bass 0.15 0.12-0.20 0.36 0.23-0.53 0.47 0.09-1.16
Sunfish 0.14 0.12-0.16 0.52 0.30-2.36 0.12 <0.06-0.71
DI Black bass 0.16 0.12-0.20 0.33 0.22-0.67 0.37 0.07-0.77
Sunfish 0.14 0.12-0.16 0.51 0.23-1.22 0.10 <0.06-0.35
DN Black bass 0.15 0.12-0.20 0.39 0.20-0.54 0.34 <0.06-0.72
Sunfish 0.14 0.12-0.15 0.48 0.33-0.66 0.14 <0.06-0.37
4.0 Summary
When considered altogether, it does not appear that any pattern of arsenic, mercury, or selenium
accumulation in fish tissues existed during the 2019-2022 NPDES permit cycle attributable to the
Asheville Plant operations or in the French Broad River within the reach sampled. Furthermore,
no fish tissue from any location or from any year exceeded the USEPA recreational screening
values for arsenic or selenium. Fish having tissue that did exceed the screening value for mercury
appear to be randomly distributed and unrelated to plant operations. The mercury results seen
during this NPDES permit cycle are also comparable to the 2004 baseline data, indicating that
mercury levels have remained relatively stable in the reach of the French Broad River sampled.
Fish tissue samples were collected every year with at least six fish from each trophic level at three
designated locations in accordance with the state approved study plan satisfying the fish tissue
monitoring requirement of the NPDES permit.
5.0 References
USEPA. 2000. Guidance for assessing chemical contaminant data for use in fish advisories.
Vol. 1. Fish sampling and analysis. Third edition. EPA 823-B-00-007. United States
Environmental Protection Agency, Office of Water, Washington, DC.
4

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Figure 1. French Broad River trace element monitoring locations.
5

Appendix A
Arsenic, mercury,and selenium concentrations(wet weight) in axial muscle of fish from the
French Broad River 2019-2022. Exeeedances of screening values are highlighted.
2022
Fish Species Locations Month Length Weight As(µgig) Hg()ig/g) Se(µg/g) Dry-to-Wet'
(MM) (9) Weight Ratio
Smallmouth Bass UP June 335 432 0.17 0.74 0.32 0.20
Smallmauth Bass UP June 236 169 0.15 0.29 0.33 0.20
Smallmouth Bass UP October 299 339 0.15 0.56 0.35 0.20
Smallmouth Bass UP October 192 104 0.14 0.18 0.26 0.20
Alabama Bass UP June 230 164 0.14 0.30 0.43 0.21
Rock Bass UP June 212 230 0.15 0.45 0.36 0.20
Largemouth Bass UP June 334 493 0.15 0.25 0.30 0.20
Largemouth Bass UP June 248 188 0.15 0.21 0.28 0.20
Largemouth Bass UP June 342 550 0.12 0.37 0.31 0.20
Largemouth Bass UP October 279 319 0.15 0.62 0.36 0.21
Redbreast Sunfish UP June 171 114 0.15 0.07 0.33 0.20
Redbreast Sunfish UP June 170 93 0.15 0.27 0.46 0.20
Redbreast Sunfish UP June 199 148 0.13 0.10 0.38 0.19
Redbreast Sunfish UP June 200 169 0.12 0.19 2.36 0.20
Redbreast Sunfish UP June 173 106 0.15 0.14 0.39 0.21
Redbreast Sunfish UP June 175 114 0.14 <0.06 0.38 0.20
Redbreast Sunfish UP June 161 82 0.15 0.09 OA4 0.20
Bluegill UP June 185 118 0.15 0.08 0.30 0.19
Bluegill UP June 177 113 0,14 0.28 0.36 0.20
Redear Sunfish UP June 208 176 0.16 0.11 0.46 0.21
Smallmouth Bass DI June 360 762 0.16 0.26 0.26 0.21
Smallmouth Bass DI June 310 437 0.16 0.35 0.32 0.21
Smallmouth Bass DI June 194 115 0.16 0.19 0.33 0.21
Smallmouth Bass DI June 400 851 0.15 0.51 0.24 0.20
Smallmouth Bass DI June 228 164 0.16 0.24 0.34 0.21
Smallmouth Bass DI October 270 271 0.16 0.16 0.26 0.21
Smallmouth Bass DI October 281 307 0.15 0.23 0.27 0.21
Rock Bass DI June 154 79 0.14 0.09 0.40 0.20
Rock Bass DI June 161 93 0.12 0.07 0.30 0.19
Rock Bass DI October 220 202 0.16 0.30 0.29 0.21
Redbreast Sunfish D1 June 194 144 0.14 0.08 0.39 0.20
Redbreast Sunfish DI June 155 73 0.13 0.07 0.48 0.20
Redbreast Sunfish DI June 143 53 0.14 0.13 0.39 0.20
Redbreast Sunfish DI June 139 54 0.14 0.11 0.52 0.20
Redbreast Sunfish DI June 162 102 0.15 0.08 0.40 0.21
Redbreast Sunfish DI June 145 75 0.12 0.06 0.29 0.17
Redbreast Sunfish DI October 138 45 0.13 0.06 0.34 0.19
Redbreast Sunfish DI October 136 43 0.13 0.13 0.36 0.19
Bluegill D1 June 177 115 0.13 0.08 0.25 0.19
Bluegill DI June 141 58 0.14 <0.06 0.86 0.21
Smallmouth Bass DN June 303 377 0.l6 0.38 0.36 0.21
Smallmouth Bass DN June 420 906 0.l4 0.63 0.34 0.20
Smallmouth Bass DN June 364 622 0,16 0.72 0.34 0.20
Smallmouth Bass DN June 235 175 0.15 0.20 0.33 0.21
Smallmouth Bass DN June 243 201 0.16 0.18 0.31 0.21
Rock Bass DN June 175 106 0.14 0.07 0.34 0.21
Largemouth Bass DN June 406 1089 0.13 0.50 0.20 0.18
Largemouth Bass DN June 454 1100 0.13 0.72 0,38 0.17
Largemouth Bass DN June 251 216 0.13 0.22 0.29 0.19
Largemouth Bass DN June 243 176 0.13 0.17 0.29 0.19
Redbreast Sunfish DN June 145 69 0.14 0.15 0.53 0.20
Redbreast Sunfish DN June 212 182 0.13 0.17 0.44 0.19
Redbreast Sunfish DN June 219 214 0.14 0.21 0.37 0.19
Redbreast Sunfish DN June 201 167 0.14 0.22 0.39 0.20
Redbreast Sunfish DN June 175 125 0.14 <0.06 0.33 0.21
Redbreast Sunfish DN June 213 211 0.15 0.27 0.45 0.21
Bluegill DN June 138 61 0.15 0.07 0.33 0.21
Bluegill DN June 144 61 0.15 <0.06 0.40 0.21
Bluegill DN June 140 65 0.14 0.06 0.37 0.20
Redear Sunfish DN June 170 92 0.15 0.I 1 0.59 0.21

2021
Fish Species Locations Month Length Weight As(µg/g) Hg(µg/g) Se(µg/g) Dry-to-Wet'
(MM) (g) _ Weight Ratio
Redbreast Sunfish UP June 182 135 0.14 0.71 0.62 0.20
Redbreast Sunfish UP June 190 145 0.13 0.23 0.46 0.19
Redbreast Sunfish UP June 187 142 0.13 0.10 0.51 0.19
Redbreast Sunfish UP June 171 106 0.14 0.09 0.40 0.20
Redbreast Sunfish UP June 197 156 0,14 0.12 0,42 0.20
Redbreast Sunfish UP June 185 143 0.13 0.07 0.42 0.19
Redbreast Sunfish UP June; 188 145 0.16 0.14 0.42 0.20
Redbreast Sunfish UP June 180 139 0.16 0.09 0.60 0.20
Redbreast Sunfish UP June 200 179 0.14 0.17 0.56 0.20
Redbreast Sunfish UP June 185 154 0.15 0.09 0.42 0.19
Smallmouth Bass UP June 306 358 0.14 0.74 0.41 0.17
Smallmouth Bass UP June 286 324 0.15 0.75 0.40 0.19
Smallmouth Bass UP June 275 336 0.17 0.65 0.36 0,21
Smallmouth Bass UP June 326 471 0.16 1.09 0.36 010
Smallmouth Bass UP June 377 791 0.16 0.45 0.30 0.20
Rock Bass UP June 225 288 0.15 0.52 0.38 0.19
Smallmouth Bass UP August 373 709 0.20 0.75 0.35 0.22
Smallmouth Bass UP August 370 624 0,20 1.16 0.46 0.22
Smallmouth Bass UP August 265 246 0.17 0.76 0.42 0.21
Smallmouth Bass UP August 200 115 0.15 0.21 0.38 0.21
Redbreast Sunfish D1 June 196 165 0.15 0.35 0.48 0.22
Redbreast Sunfish DI June 202 193 0.14 0.21 0.48 0.20
Redbreast Sunfish DI June 165 106 0.15 0.12 0.74 0.21
Redbreast Sunfish DI June 171 106 0.14 0.06 0.44 0.20
Bluegill DI June 148 66 0.14 0.07 0.42 0.20
Redbreast Sunfish DI June 156 59 0.13 0.13 0.49 0.18
Redbreast Sunfish DI June 151 65 0.14 0.09 0.64 0.20
Bluegill DI June 150 67 0.13 0.06 0.30 0.19
Bluegill DI June 139 58 0.13 0.06 0.34 0.19
Redbreast Sunfish DI June 125 38 0.16 0.07 0.72 0.20
Smallmouth Bass DI June 425 1032 0.15 0.53 0.23 0.19
Smallmouth Bass DI June 314 390 0.15 0.56 0.27 0.19
Smallmouth Bass DI June 387 792 0.16 0.74 0.24 0.20
Smallmouth Bass DI June 370 697 0.17 0.67 0.42 0.21
Largemouth Bass DI June 178 83 0.14 0.10 0.34 0.20
Largemouth Bass DI June 212 124 0.14 0.25 0.36 0.20
Alabama Bass DI June 390 660 0.17 0.28 0.67 0.21
Smallmouth Bass DI June 410 952 0.16 0.57 0.22 0.20
Smallmouth Bass DI June 445 1142 0.14 0.54 0.23 0.18
Redbreast Sunfish DN June 209 190 0.14 0.07 0.42 0.20
Redbreast Sunfish DN June 216 200 0.14 0,09 0.40 0.20
Redbreast Sunfish DN June 225 237 0.14 0.37 0.50 0.20
Redbreast Sunfish DN June 208 179 0.13 0.25 0.53 0.19
Redbreast Sunfish DN June 209 184 0.13 0.12 0.42 0.19
Redbreast Sunfish DN June 192 145 0.13 0.18 0.42 0.19
Redbreast Sunfish DN June 177 100 0.13 0.17 0.42 0.19
Redbreast Sunfish DN June 179 113 0.14 0.35 0.48 0.20
Redbreast Sunfish DN June 212 197 0.14 0.20 0.54 0.20
Redbreast Sunfish DN June 158 80 0.14 0.12 0.42 0.20
Rock Bass DN June 205 167 0.15 0.13 0.51 0.19
Rock Bass DN June 191 134 0.15 0.16 0.36 0.19
Rock Bass DN June 212 205 0.14 0.21 0,46 0.20
Smallmouth Bass DN June 274 292 0.13 0.41 0.38 0.19
Smallmouth Bass DN June 277 289 0.16 0.45 0.46 0.20
Smallmouth Bass DN June 354 617 0.16 0.60 0.36 0.20
Smallmouth Bass DN June 349 560 0.15 0.63 0.34 0.19
Smallmouth Bass DN August 378 588 0.17 0.65 0,40 0.21
Smallmouth Bass DN August 275 218 0.16 0.22 0.36 0.20
Largemouth Bass DN August 360 634 0.13 0.20 0.31 0.18

2020
Fish Species Locations Month Length Weight As(µg/g) Hg(µg/g) Se(µg/g) Dry-to-Wet'
(MM) (g) Weight Ratio
Redbreast Sunfish UP June 187 138 0.15 0.08 0.41 0.21
Redbreast Sunfish UP June 188 135 0.14 0.1 0.65 0.20
Redbreast Sunfish UP June 190 142 0.14 0.09 0.56 0.20
Redbreast Sunfish UP June 188 143 0.14 0.11 0.39 0.20
Redbreast Sunfish UP June 184 113 0.15 0.11 0.46 0.20
Redbreast Sunfish UP June 193 142 0,13 0.08 0.51 0.19
Redbreast Sunfish UP September 168 86 0.15 0.13 0.48 0,20
Redbreast Sunfish UP September 181 118 0.15 0.09 0.41 0.19
Redbreast Sunfish UP September 169 91 0.15 0,07 0.44 0.21
Redear Sunfish UP September 185 104 0.16 0.12 0.57 0.21
Smallmouth Bass UP June 311 410 0.16 0.72 0.44 0.19
Smallmouth Bass UP June 267 259 0.15 0.93 0.49 0.20
Rock Bass UP June 195 155 0.15 0.09 0.49 0.20
Rock Bass UP June 186 142 0.19 0.16 0.53 0.23
Largemouth Bass UP June 344 568 0.15 0.29 0.44 0.19
Largemouth Bass UP June 378 933 0.15 0.35 0.29 0.19
Largemouth Bass UP September 297 402 0.14 0.21 0.31 0.20
Bluegill DI June 145 61 0.14 0.06 0.40 0.20
Bluegill DI June 140 53 0.13 0.07 0.35 0.19
Bluegill Dt September 172 113 0.16 0.10 0.70 0.20
Redbreast Sunfish DI September 185 128 0.13 0.09 0.48 0.19
Redbreast Sunfish DI September 205 164 0.14 0.16 0.58 0.19
Redbreast Sunfish DI September 150 70 0.13 0.07 0.47 0.19
Smallmouth Bass DI June 170 170 0.15 0.14 0.38 0.20
Smallmouth Bass DI June 215 215 0.l5 0.43 0.43 0.20
Smallmouth Bass DI June 237 237 0.15 0.21 0.40 0.20
Smallmouth Bass DI June 230 230 0.15 0.14 0.33 0.20
Smallmouth Bass DI September 365 365 0.19 0.42 0.34 0.22
Largemouth Bass DI June 182 182 0.13 0.15 0.29 0.19
Redbreast Sunfish DN June 185 166 0.14 0.16 0.65 0.20
Redbreast Sunfish DN June 196 172 0.15 0.08 0.58 0.21
Redbreast Sunfish DN June 200 193 0.14 0.09 0.54 0.20
Redbreast Sunfish DN June 213 201 0.13 0.08 0.55 0.20
Redbreast Sunfish DN June 206 220 0.14 0.09 0.47 0.20
Redbreast Sunfish DN September 211 130 0.12 0,14 0,51 0.19
Redbreast Sunfish DN September 190 150 0.13 0.14 0.5 0.19
Redbreast Sunfish DN September 196 106 0.15 0,16 0.56 0.20
Redbreast Sunfish DN September 177 93 0.12 0.07 0.34 0.18
Bluegill DN June 164 166 0.14 0.16 0.65 0.20
Smallmouth Bass DN June 228 145 0.20 0.15 0.16 0.46
Smallmouth Bass DN June 241 185 0.19 0.23 0.14 0.48
Smallmouth Bass DN June 239 171 0.20 0.18 0.15 0.48
Smallmouth Bass DN June 219 129 0.19 0.16 0.16 0.46
Smallmouth Bass DN June 252 210 0.20 0.16 0.16 0.54
Largemouth Bass DN June 257 227 0.20 0.26 O.i4 0.41
Smallmouth Bass DN September 290 247 0.24 0.42 0.20 0.50

2019
Fish Species Locations Month Length Weight As(µg/g) Hg(pg/g) Se(pg/g) Dry-to-Wet'
(MM) (g) _ _ _ Weight Ratio
Redbreast Sunfish UP June 190 159 0.15 0.08 0.47 0.20
Redbreast Sunfish UP June 190 149 0.13 0.07 0.59 0.19
Redbreast Sunfish UP June 196 158 0.14 <0,06 0.46 0.20
Redbreast Sunfish UP June 203 166 0.15 0.06 0.46 0.20
Redbreast Sunfish UP June 186 138 0.14 0.08 0.49 0.20
Redbreast Sunfish UP June 180 123 0.l4 <0.06 0.65 0.21
Largemouth Bass UP October 488 1700 0.16 0.52 0.44 0.20
Largemouth Bass UP October 488 1700 0.15 0.18 0.33 0,20
Largemouth Bass UP October 415 1225 0.15 0.28 0.28 0.20
Bluegill UP November 184 130 0.16 <0.06 0.49 0.20
Redear Sunfish UP November 184 118 0.16 0.09 0.65 0,21
Redbreast Sunfish UP November 171 80 0.14 0.07 0.45 0.20
Largemouth Bass UP November 286 278 0.13 0.18 0.26 0.20
Largemouth Bass UP November 280 246 0.14 0.17 0.30 0.20
Largemouth Bass UP November 349 636 0.14 0.33 0.23 0.20
Redbreast Sunfish DI June 182 135 0.14 0.07 0.47 0.20
Redbreast Sunfish DI June 178 99 0.13 0.08 1,06 0,19
Redbreast Sunfish DI June 165 84 0.14 0,08 0,62 0.19
Redbreast Sunfish DI October 200 128 0.13 0.07 0.23 0.20
Redbreast Sunfish ❑I October 200 128 0.13 0.15 0.84 0.19
Redbreast Sunfish D1 October 188 122 0.14 0.14 0,44 0.19
Redbreast Sunfish DI October 182 91 0.12 0.11 1.27 0.19
Redbreast Sunfish DI October 179 94 0.14 0.10 0.36 0.19
Smallmouth Bass DI June 384 703 0.19 0.47 0.48 0.21
Smallmouth Bass DI June 306 297 0,15 0,80 0,49 0,19
Smallmouth Bass DI June 290 291 0,16 0,28 0,32 0,20
Smallmouth Bass DI October 438 1016 0,16 0.51 0.21 Ul
Smallmouth Bass DI October 283 274 0.17 0.37 0.29 0.21
Smallmouth Bass DI October 473 1406 0.17 0.65 0.30 0.22
Redbreast Sunfish DN October 180 91 0.14 <0.06 0.55 0.21
Redbreast Sunfish DN October 192 122 0,15 0.37 0,60 0.18
Redbreast Sunfish DN October 193 125 0.15 0.09 0.58 0.20
Redbreast Sunfish DN October 200 156 0.14 0.07 0.52 0.20
Redbreast Sunfish DN October 178 99 0.13 0.14 0.62 0.21
Redbreast Sunfish DN October 186 122 0.13 0.08 0,60 0,19
Redbreast Sunfish DN October 221 172 0,14 0,08 0.56 0.19
Bluegill DN October 193 150 0.13 0.10 0.35 0.20
Largemouth Bass DN October 327 460 0.14 0.21 0.37 0.20
Largemouth Bass DN October 268 217 0.14 0.19 0.41 0.19
Smallmouth Bass DN October 395 768 0.14 <0.06 0.34 0.20
Smallmouth Bass DN October 329 422 0.15 0.53 0,41 0.21
Smallmouth Bass DN October 385 592 0,16 0.69 0.45 0.19
Smal[mouth Bass DN October 245 177 0,15 0.12 0.54 0.20
Smallmouth Bass DN October 395 768 0.17 0.61 0.36 0.22
'To convert to a dry weight,divide the wet weight concentrations by the dry-to-wet weight ratio.

Appendix B
Baseline mercury and selenium concentrations (wet weight) in axial muscle of fish
from the French Broad River during August and November 2004.1 Exceedances
of screening values are highlighted.
Locations Month Length Weight Hg Se Dry-to-Wet'
Fish (MM) (g) (µg/g) (µg/g) Weight Ratio
Species
Smallmouth bass UP August 246 201 0.87 0.39 0.22
Smallmouth bass UP August 297 370 0.49 0.36 0.21
Smallmouth bass UP August 346 620 0.44 0.22 0.22
Smallmouth bass UP August 445 1,300 1.66 0.31 0.24
Smallmouth bass UP August 370 744 1.49 0.30 0.20
Redbreast sunfish UP August 172 106 <0.18 0.28 0.20
Redbreast sunfish UP August 160 87 0.32 0.24 0.20
Redbreast sunfish UP August 145 72 <0.27 0.39 0.30
Redbreast sunfish UP August 149 60 <0.17 0.25 0.21
Redbreast sunfish UP August 190 160 0.12 0.32 0.20
Black redhorse UP August 372 540 <0.15 0.26 0.21
Black redhorse UP August 380 550 <0.17 0.19 0.21
Black redhorse UP August 410 790 0.53 0.26 0.20
Black redhorse UP August 413 843 0.54 0.24 0.19
Black redhorse UP August 415 999 0.40 0.30 0.20
Black redhorse UP August 405 863 0.46 0.30 0.20
Largemouth bass DI August 475 1,725 0.74 1.23 0.21
Largemouth bass DI August 395 611 <0.16 1.46 0.20
Largemouth bass DI August 405 795 0.17 0,21 0,21
Smallmouth bass DI August 263 223 0.52 0.32 0.22
Smallmouth bass DI August 355 565 0.54 4.14 0.22
Smallmouth bass DI August 374 871 0.27 0.47 0,23
Smallmouth bass DI August 368 802 0.74 0.25 0.22
Smallmouth bass DI August 440 1,300 0,99 0.45 0.22
Redbreast sunfish DI August 136 52 <0.19 3.81 0.21
Redbreast sunfish DI August 183 108 <0.17 3.61 0.19
Redbreast sunfish DI August 164 80 0.20 0.57 0.20
Redbreast sunfish DI August 182 128 <0.15 0.69 0.19
Redbreast sunfish DI August 177 109 0.74 1,23 0.20
Redbreast sunfish DI August 149 58 <0.16 1,46 0.20
Black redhorse DI August 375 <0.18 0.26 0.30 0.22
Black redhorse DI August 383 0.25 0.25 0.30 0.21
Black redhorse DI August 457 0.52 0.48 0.50 0.20
Black redhorse DI August 465 0.76 0.21 0.20 0,21
Black redhorse DI August 493 0.69 0.21 0.20 0.21
Black redhorse DI August 475 0,43 0.77 0.80 0,20
Black redhorse DI November 410 <0.18 0.26 0.20 0.20
Smallmouth bass DN November 277 265 0.29 0.57 0.21
Smallmouth bass DN November 295 410 0.32 0.15 0,21
Smallmouth bass DN November 310 460 0.77 0.35 0.22
Smallmouth bass DN November 347 620 0.68 0.33 0.22
Smalimouth bass DN November 345 750 0.40 0.29 0.22
Bluegill DN August 135 59 <0,16 0.33 0.20
Redbreast sunfish DN August 162 66 0.32 0.83 0,20
Redbreast sunfish DN August 175 96 <0.18 0.76 0.22
Redbreast sunfish DN August 185 139 0.25 0.31 0.19
Redbreast sunfish DN August 198 156 <0,15 0.27 0.19
Redbreast sunfish DN August 273 324 0.54 0.19 0.21
Redbreast sunfish DN August 126 46 <0.14 0.41 0,20
Redbreast sunfish DN August 197 170 0.l2 0.73 0.20
Redbreast sunfish DN August 198 154 <0.16 0.93 0.20
Black redhorse DN August 365 509 <0.2 0.50 0.20
Black redhorse DN August 356 518 0.1 0.30 0.20
Black redhorse DN August 375 651 <0.2 0.80 0.20
Black redhorse DN August 395 755 <0.2 0.50 0.20
Black redhorse DN August 388 810 0.5 0.40 0.21
Black redhorse DN August 423 910 <0.2 0.40 0.19
Arsenic was not required by permit during baseline monitoring.
Z To convert to a dry weight,divide the wet weight concentrations by the dry-to-wet weight ratio.