NC0021717_Renewal Application_20190403

ROY COOPER
GM,crnor
MICHAEL S. REGAN
Scrrctor r
LINDA CULPEPPER
Oite(for
Kenneth Noland, Manager Town
Town of Wilkesboro
203 W Main St
Wilkesboro, NC 28697
Subject: Permit Renewal
Application No. NCO021717
Cub Creek WWTP
Wilkes County
Dear Applicant:
NORTH CAROLINA
Environmental Quality
April 03, 2019
The Water Quality Permitting Section acknowledges the April 2, 2019 receipt of your permit renewal application and
supporting documentation. Your application will be assigned to a permit writer within the Section's NPDES WW permitting
branch. Per G.S. 150B-3 your current permit does not expire until permit decision on the application is made.
Continuation of the current permit is contingent on timely and sufficient application for renewal of the current permit. The
permit writer will contact you if additional information is required to complete your permit renewal. Please respond in a
timely manner to requests for additional information necessary to allow a complete review of the application and renewal
of the permit.
Information regarding the status of your renewal application can be found online using the Department of Environmental
Quality's Environmental Application Tracker at:
https•//deq nc gov/permits-regulations/permit-guidance/environmental-application-tracker
If you have any additional questions about the permit, please contact the primary reviewer of the application using the
links available within the Application Tracker.
Sincerely,
A�(av �0�
Wren Thedford
Administrative Assistant
Water Quality Permitting Section
ec: WQPS Laserfiche File w/application
E North Cara in Department of Environmento] Qua v. I D vs• r, of Water Fesour-•s
W rstorSa err, Regors MDe 1 450 West Hanes A' Road, Su le 3DD I W rstorSa em, North Carona 27105
'"�� 336 776-98DD

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717 2019 Renewal Yadkin
FORM
2A NPDES FORM 2A APPLICATION OVERVIEW
NPDES
APPLICATION OVERVIEW
Form 2A has been developed in a modular format and consists of a "Basic Application Information" packet
and a "Supplemental Application Information" packet. The Basic Application Information packet is divided
into two parts. All applicants must complete Parts A and C. Applicants with a design flow greater than or
equal to 0.1 mgd must also complete Part B. Some applicants must also complete the Supplemental
Application Information packet. The following items explain which parts of Form 2A you must complete.
BASIC APPLICATION INFORMATION: Basic Application Information for all Applicants. All applicants must complete
questions A.1 through A.8. A treatment works that discharges effluent to surface waters of the United States must also
answer questions A.9 through A.12.
A. Additional Application Information for Applicants with a Design Flow >_ 0.1 mgd. All treatmep,k� flows
greater than or equal to 0.1 million gallons per day must complete questions B.1 through B.6. REur-`�� i�� '�
s2F�at 1
B. Certification. All applicants must complete Part C (Certification). Ar r� 0 2 2019
SUPPLEMENTAL APPLICATION INFORMATION: Water ( j4,ources
P®rmit:ing Section
C. Expanded Effluent Testing Data. A treatment works that discharges effluent to surface waters of the United States and meets
one or more of the following criteria must complete Part D (Expanded Effluent Testing Data):
1. Has a design flow rate greater than or equal to 1 mgd,
2. Is required to have a pretreatment program (or has one in place), or
3. Is otherwise required by the permitting authority to provide the information.
D. Toxicity Testing Data. A treatment works that meets one or more of the following criteria must complete Part E (Toxicity Testing
Data):
1. Has a design flow rate greater than or equal to 1 mgd,
2. Is required to have a pretreatment program (or has one in place), or
3. Is otherwise required by the permitting authority to submit results of toxicity testing
E. Industrial User Discharges and RCRA/CERCLA Wastes. A treatment works that accepts process wastewater from any
significant industrial users (SIUs) or receives RCRA or CERCLA wastes must complete Part F (Industrial User Discharges
and RCRA/CERCLA Wastes). SIUs are defined as:
1. All industrial users subject to Categorical Pretreatment Standards under 40 Code of Federal Regulations (CFR) 403.6 and
40 CFR Chapter I, Subchapter N (see instructions), and
2. Any other industrial user that:
a. Discharges an average of 25,000 gallons per day or more of process wastewater to the treatment works (with certain
exclusions), or
b. Contributes a process wastestream that makes up 5 percent or more of the average dry weather hydraulic or organic
capacity of the treatment plant, or
C. Is designated as an SIU by the control authority.
G. Combined Sewer Systems. A treatment works that has a combined sewer system must complete Part G (Combined Sewer
Systems).
ALL APPLICANTS MUST COMPLETE PART C (CERTIFICATION)
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 1 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
BASIC APPLICATION INFORMATION
PART A. BASIC APPLICATION INFORMATION FOR ALL APPLICANTS:
All treatment works must complete questions A.1 through A.8 of this Basic Application Information Packet.
A.1. Facility Information.
Facility Name Wilkesboro Water Reclamation Facility (formerly Town of Wilkesboro Cub Creek Wastewater Treatment Plant)
Mailing Address P. O Box 1056
Wilkesboro NC 28697
Contact Person Ken Noland
Title Town Manager
Telephone Number 336-838-3951
Facility Address 700 Snyder Street
(not P.O. Box) Wilkesboro NC 28697
A.2. Applicant Information. If the applicant is different from the above, provide the following:
Applicant Name
Mailing Address
Contact Person
Title
Telephone Number _ L
Is the applicant the owner or operator (or both) of the treatment works?
® owner ® operator
Indicate whether correspondence regarding this permit should be directed to the facility or the applicant.
❑ facility ® applicant
A.3. Existing Environmental Permits. Provide the permit number of any existing environmental permits that have been issued to the treatment works
(include state -issued permits).
NPDES NCO021717 PSD
UIC Other
RCRA Other
A.4. Collection System Information. Provide information on municipalities and areas served by the facility. Provide the name and population of each
entity and, if known, provide information on the type of collection system (combined vs separate) and its ownership (municipal, private, etc.).
Name Population Served Type of Collection System Ownership
Town of Wilkesboro NC 4,000 separate sewer Town of Wilkesboro
Total population served 4,000
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22, Page 2 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN.
Town of Wilkesboro Cub Creek, NCO021717 2019 Renewal Yadkin
A.5. Indian Country.
a- Is the treatment works located in Indian Country?
❑ Yes ® No
b. Does the treatment works discharge to a receiving water that is either in Indian Country or that is upstream from (and eventually flows
through) Indian Country?
❑ Yes ❑ No
A.6. Flow. Indicate the design flow rate of the treatment plant (i e., the wastewater flow rate that the plant was built to handle). Also provide the
average daily flow rate and maximum daily flow rate for each of the last three years. Each year's data must be based on a 12-month time period
with the 1211 month of "this year" occurring no more than three months prior to this application submittal
a. Design flow rate 4.90 mgd
Two Years Ago Last Year This Year
b. Annual average daily flow rate 3.66 MGD [CY 20161 3.99 MGD [CY 20171 4.13 MGD [CY 20181
c Maximum daily flow rate 6.20 MGD [CY 20161 8.50 MGD [CY 20171 7.64 MGD [CY 20181
A.7. Collection System. Indicate the type(s) of collection system(s) used by the treatment plant. Check all that apply. Also estimate the percent
contribution (by miles) of each.
Separate sanitary sewer 100 %
❑ Combined storm and sanitary sewer 0 %
A.B. Discharges and Other Disposal Methods.
a. Does the treatment works discharge effluent to waters of the U.S ? ® Yes ❑ No
If yes, list how many of each of the following types of discharge points the treatment works uses:
i Discharges of treated effluent
ii. Discharges of untreated or partially treated effluent
iii. Combined sewer overflow points
iv. Constructed emergency overflows (prior to the headworks)
V. Other
b Does the treatment works discharge effluent to basins, ponds, or other surface impoundments
that do not have outlets for discharge to waters of the U.S.? ❑ Yes
If yes, provide the following for each surface impoundment:
Location:
Annual average daily volume discharge to surface impoundment(s)
Is discharge 0 continuous or ❑ intermittent?
C. Does the treatment works land -apply treated wastewater?
If yes, provide the following for each land application site:
d.
Location:
Number of acres:
Annual average daily volume applied to site:
Is land application ❑ continuous or ❑ intermittent?
Does the treatment works discharge or transport treated or untreated wastewater to another
treatment works?
1 (one)
0 (none)
0 (none)
0 (none)
® No
mgd
❑ Yes ® No
mgd
❑ Yes ❑ No
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 3 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
If yes, describe the mean(s) by which the wastewater from the treatment works is discharged or transported to the other treatment works
(e-g., tank truck, pipe)
If transport is by a party other than the applicant, provide:
Transporter Name
Mailing Address
Contact Person
Title
Telephone Number
For each treatment works that receives this discharge, provide the following:
Name
Mailing Address
Contact Person _
Title
Telephone Number L_ 1
If known, provide the NPDES permit number of the treatment works that receives this discharge
Provide the average daily flow rate from the treatment works into the receiving facility.
mgd
e. Does the treatment works discharge or dispose of its wastewater in a manner not included
in A.8 through A 8 d above (e.g , underground percolation, well injection): ❑ Yes
© No
If yes, provide the following for each disposal method:
Description of method (including location and size of site(s) if applicable):
Annual daily volume disposed by this method: _
Is disposal through this method ❑ continuous or ❑ intermittent?
EPA Form 3510-2A (Rev- 1-99). Replaces EPA forms 7550-6 & 7550-22 Page 4 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717 2019 Renewal Yadkin
WASTEWATER DISCHARGES:
If you answered "Yes" to question A.8.a, complete questions A.9 through A.12 once for each outfall (including bypass points) through
which effluent is discharged. Do not include information on combined sewer overflows in this section. If you answered "No" to question
A.8.a, go to Part B, "Additional Application Information for Applicants with a Design Flow Greater than or Equal to 0.1 mgd."
A.9. Description of Outfall.
a. Outfall number 001
b- Location WILKESBORO
28697
(City or town, if applicable)
(Zip Code)
WILKES
NORTH CAROLINA
(County)
(State)
36'09'19"
80°08'20"
(Latitude)
(Longitude)
c Distance from shore (if applicable)
NOT APPLICABLE ft
d. Depth below surface (if applicable)
NOT APPLICABLE ft.
e. Average daily flow rate [CY2018]
4.13 mgd
f Does this outfall have either an intermittent or a periodic discharge?
❑ Yes ® No (go to A.9.g-)
If yes, provide the following information:
Number f times per year discharge occurs:
Average duration of each discharge:
Average flow per discharge:
Months in which discharge occurs:
g. Is outfall equipped with a diffuser?
A.10. Description of Receiving Waters.
❑ Yes ® No
mgd
a. Name of receiving water Yadkin River
b. Name of watershed (if known) YADKIN/PEE DEE RIVER BASIN
United States Soil Conservation Service 14-digit watershed code (if known): 03040101040010
c. Name of State Management/River Basin (if known): YADKIN RIVER BASIN
United States Geological Survey 8-digit hydrologic cataloging unit code (if known): 03040101
d. Critical low flow of receiving stream (if applicable)
acute N/A cfs chronic Summer 7Q10 = X.X PT MEMO cfs
e. Total hardness of receiving stream at critical low flow (if applicable): NOT APPLICABLE mg/I of CaCO3
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 5 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
A.11. Description of Treatment
a. What level of treatment are provided? Check all that apply.
❑ Primary ® Secondary
❑ Advanced ❑ Other. Describe:
b. Indicate the following removal rates (as applicable):
Design BOD5 removal or Design CBOD5 removal 90 %
Design SS removal 90 %
Design P removal 90 %
Design N removal 90 %
Other %
C. What type of disinfection is used for the effluent from this outfall? If disinfection varies by season, please describe:
UV Disinfection (year-round)
If disinfection is by chlorination is dechlorination used for this outfall? ❑ Yes ® No Nat Applicable
Does the treatment plant have post aeration? ® Yes ❑ No
A.12. Effluent Testing Information. All Applicants that discharge to waters of the US must provide effluent testing data for the following
parameters. Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is
discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data
collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of
40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a
minimum, effluent testing data must be based on at least three samples and must be no more than four and one-half years apart.
Outfall number: 001 [Data from CY2017 through CY20181
MAXIMUM DAILY VALUE
AVERAGE DAILY VALUE
PARAMETER
Value
Units
Value
Units
Number of Samples
pH (Minimum)
6.6
S.U.
pH (Maximum)
7.8
S.U.
Flow Rate
8.5
MGD
4.06
MGD
730
Temperature (winter) [11/1 - 3/31]
XX.X
°C
10.06
°C
201
Temperature (Summer) [4/1 - 10/31] XX.X °C 22.66 cc 298
• For pH please report a minimum and a maximum daily value
MAXIMUM DAILY
AVERAGE DAILY DISCHARGE
POLLUTANT
DISCHARGE
ANALYTICAL
ML/MDL
of
METHOD
Conc.
FNumber
Units
Conc.
Units
Samples
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
BIOCHEMICAL OXYGEN
BODS
20.7
m /1
8.60
Mg/1
492
SM 5210 B
2 mg/1
DEMAND (Report one)
CBOD5
FECAL COLIFORM ['geometric mean]
20C
1100 ml
11.82
/100 ml
451
SM 9222 D
1 c01/100 m1
TOTAL SUSPENDED SOLIDS (TSS)
737
un/I
15.52
m /l
494
SM 2540 D
1 m /I
END OF PART A.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 6 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
BASIC APPLICATION INFORMATION
PART B. ADDITIONAL APPLICATION INFORMATION FOR APPLICANTS WITH A DESIGN FLOW GREATER THAN OR
EQUAL TO 0.1 MGD (100,000 gallons per day).
All applicants with a design flow rate >_ 0.1 mgd must answer questions B.1 through B.6. All others go to Part C (Certification).
B.1. Inflow and Infiltration. Estimate the average number of gallons per day that flow into the treatment works from inflow and/or infiltration.
750,000 gpd (During a'/;' or more rainfall event)
Briefly explain any steps underway or planned to minimize inflow and infiltration.
Kimberly call Bob
B.2. Topographic Map. Attach to this application a topographic map of the area extending at least one mile beyond facility property boundaries. This
map must show the outline of the facility and the following information. (You may submit more than one map if one map does not show the entire
area )
a. The area surrounding the treatment plant, including all unit processes
b. The major pipes or other structures through which wastewater enters the treatment works and the pipes or other structures through which
treated wastewater is discharged from the treatment plant. Include ouffalls from bypass piping, if applicable.
c. Each well where wastewater from the treatment plant is injected underground.
d. Wells, springs, other surface water bodies, and drinking water wells that are: 1) within '/. mile of the property boundaries of the treatment
works, and 2) listed in public record or otherwise known to the applicant.
e. Any areas where the sewage sludge produced by the treatment works is stored, treated, or disposed.
f. If the treatment works receives waste that is classified as hazardous under the Resource Conservation and Recovery Act (RCRA) by truck, rail,
or special pipe, show on the map where the hazardous waste enters the treatment works and where it is treated, stored, and/or disposed.
B.3. Process Flow Diagram or Schematic. Provide a diagram showing the processes of the treatment plant, including all bypass piping and all
backup power sources or redunancy in the system. Also provide a water balance showing all treatment units, including disinfection (e.g.,
chlorination and dechlorination). The water balance must show daily average flow rates at influent and discharge points and approximate daily flow
rates between treatment units. Include a brief narrative description of the diagram. {Schematic and Narrative Attached]
B.4. Operation/Maintenance Performed by Contractor(s).
Are any operational or maintenance aspects (related to wastewater treatment and effluent quality) of the treatment works the responsibility of a
contractor? ❑ Yes [7 No
If yes, list the name, address, telephone number, and status of each contractor and describe the contractor's responsibilities (attach additional
pages if necessary)
Name:
Mailing Address:
Telephone Number
Responsibilities of Contractor:
B.5. Scheduled improvements and Schedules of Implementation. Provide information on any uncompleted implementation schedule or
uncompleted plans for improvements that will affect the wastewater treatment, effluent quality, or design capacity of the treatment works If the
treatment works has several different implementation schedules or is planning several improvements, submit separate responses to question B.5
for each. (If none, go to question B 6.)
a. List the ouffall number (assigned in question A.9) for each ouffall that is covered by this implementation schedule.
001 — One additional final clarifier is being added
b. Indicate whether the planned improvements or implementation schedule are required by local, State, or Federal agencies.
❑ Yes ® No
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 7 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
C. If the answer to B.5.b is "Yes," briefly describe, including new maximum daily inflow rate (if applicable).
d. Provide dates imposed by any compliance schedule or any actual dates of completion for the implementation steps listed below, as
applicable. For improvements planned independently of local, State, or Federal agencies, indicate planned or actual completion dates, as
applicable Indicate dates as accurately as possible.
Schedule Actual Completion
Implementation Stage MM/DD/YYYY MM/DD/YYYY
Begin Construction
End Construction
Begin Discharge
Attain Operational Level
e. Have appropriate permits/clearances concerning other Federal/State requirements been obtained? ® Yes ❑ No
Describe briefly: Authorization to Construct (AtoC) was obtained
B.6. EFFLUENT TESTING DATA (GREATER THAN 0.1 MGD ONLY).
Applicants that discharge to waters of the US must provide effluent testing data for the following parameters. Provide the indicated
effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include information
on combine sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate
QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a minimum effluent testing data must be
based on at least three pollutant scans and must be no more than four and one-half years old.
Outfall Number: 001 [Data from CY2017 through CY2018]
MAXIMUM DAILY
AVERAGE DAILY DISCHARGE
DISCHARGE
ANALYTICAL
POLLUTANT
METHOD
MUMDL
Conic.
Units
Conc.
Units
Number of
Samples
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
AMMONIA (as N)
28.5
mg/I
2.53
mg/I
497
SM 4500 NH3 F
0.1 mg/I
CHLORINE (TOTAL
Not
Not
Not Applicable
RESIDUAL, TRC)
Applicable
Applicable
DISSOLVED OXYGEN
11.2
mg/1
7.99
mg/I
493
SM 4500 O G
0.5 mg/I
TOTAL KJELDAHL
11.5
mg/I
5.5
mg/I
08
EPA 351.1
0.2 mg/I
NITROGEN (TKN)
NITRATE PLUS NITRITE
15.7
mg/I
10.50
mg/I
08
EPA 353.2
0.1 mg/I
NITROGEN
OIL and GREASE
<5
mg/I
<5
mg/I
08
EPA 413.1
5 mg/1
PHOSPHORUS (Total)
9.9
mg/I
8.90
mg/I
08
SM 4500 P E
0.1 mg/I
TOTAL DISSOLVED SOLIDS
mg/I
94.04
mg/I
494
SM 2540C
10 mg/I
(TDS)
OTHER
END OF PART B.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22 Page 8 of 22

I
�1 J N. C. 7
VACPAJIGIC L66fjON
no
1002
q
i
0
i
0
N.
7'30"
W OlftE. 81'07'30"
ROAD CLASSIFICATION •c
`, ca
JQ, �
Heavy-duty Lighl duty..
Medium duty .� Unimproved dirt .....
0 U S. Route O Stale Route
WILKESBORO, N. C.
N3607.5—W8107.5/7.5
1966
Ah1S 4756 11 NW —SERIES V042
04;1.i

H. WWTP Diagram
Influent
Pumps
gester
Diges er # 2
Clarifier #1
Sampling Points
1 --Influent #1
2- Influent #2 Tyson
3 — Activated Sludge
Basins
4 — Sludge to Disposal
6- Effluent after UV
Disinfection
Town of Wilkesboro
Headworks Long Term Monitoring Plan
Headworks
Structure _
Clarifier #2
Dip,,sl�,- # 3
tiv Effluen i
Disinfect
I G
T I
OO
Return Activated
Sludge UO
Pump Pit 0
LO
Filter Press Sludge to
Disposal
.---� Effluent Influent
_.....��) Sludge �b Digester Supernatant
--•—�► Activated Sludge Return Activated Sludge
Waste Activated Sludge Return Activated Sludge from Clarifiers
!1"
File NameModel LTMP_STMP July 2004 Page 5
I'rintr�l: February 2S. 2U1?. 12:47 I'M
Modeled after: Chapter 4, Appendix 4-A. DWO Model Revision Date: July 29, 2004

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
BASIC APPLICATION INFORMATION
PART C. CERTIFICATION
All applicants must complete the Certification Section. Refer to instructions to determine who is an officer for the purposes of this
certification. All applicants must complete all applicable sections of Form 2A, as explained in the Application Overview. Indicate below which
parts of Form 2A you have completed and are submitting. By signing this certification statement, applicants confirm that they have reviewed
Form 2A and have completed all sections that apply to the facility for which this application is submitted.
Indicate which parts of Form 2A you have completed and are submitting:
® Basic Application Information packet Supplemental Application Information packet:
® Part D (Expanded Effluent Testing Data)
® Part E (Toxicity Testing: Biomonitoring Data)
® Part F (Industrial User Discharges and RCRA/CERCLA Wastes)
❑ Part G (Combined Sewer Systems)
ALL APPLICANTS MUST COMPLETE THE FOLLOWING CERTIFICATION.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system
designed to assure that qualified personnel properly gather and evaluate the information submitted- Based on my inquiry of the person or persons who
manage the system or those persons directly responsible for gathering the information, the information is, to the best of my knowledge and belief, true,
accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment
for knowing violations. f� ►'/nvA, r
� o ri A i c� c�
Name and official title C ��Y� ✓� L) ✓� 0� � ` w A
Signature
3 ✓ 3/$ qs/
Telephone number
Date signed �l'1 f Q
i
Upon request of the permitting authority, you must submit any other information necessary to assure wastewater treatment practices at the treatment
works or identify appropriate permitting requirements.
SEND COMPLETED FORMS TO:
NCDENR/ DWQ
Attn: NPDES Unit
1617 Mail Service Center
Raleigh, North Carolina 27699-1617
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22 Page 9 of 22

FACILITY NAME AND PERMIT NUMBER:
Town of Wilkesboro Cub Creek, NC0021717
PERMIT ACTION REQUESTED:
2019 Renewal
RIVER BASIN:
Yadkin
SUPPLEMENTAL APPLICATION INFORMATION
PART D. EXPANDED EFFLUENT TESTING DATA
Refer to the directions on the cover page to determine whether this section applies to the treatment works.
Effluent Testing: 1.0 mgd and Pretreatment Works. If the treatment works has a design flow greater than or equal to 1.0 mgd or it has (or is required
to have) a pretreatment program, or is otherwise required by the permitting authority to provide the data, then provide effluent testing data for the following
pollutants Provide the indicated effluent testing information and any other information required by the permitting authority for each ouffall through which
effluent is discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected
through analyses conducted using 40 CFR Part 136 methods. In addition, these data must comply with QA/QC requirements of 40 CFR Part 136 and
other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136 Indicate in the blank rows provided below
any data you may have on pollutants not specifically listed in this form. At a minimum, effluent testing data must be based on at least three pollutant
scans and must be no more than four and one-half years old.
Ouffall number: 001 fCY2018-CY20181 (Complete once for each outall discharging effluent to waters of the United States.)
POLLUTANT
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
ANALYTICAL
METHOD
ML/MDL
Conc.
Units
Mass
Units
Conc.
Units
Mass
Units
Number
of
Samples
METALS (TOTAL RECOVERABLE), CYANIDE, PHENOLS, AND HARDNESS.
ANTIMONY
<25
Pg/I
<25
Pg/1
3
EPA 200.7
25 pg/I
ARSENIC
<10
Pg/I
<10
Pg/I
3
EPA 200.7
5 pg/I
BERYLLIUM
<5
Pg/I
<5
pg/I
3
EPA 2007
5 pg/1
CADMIUM
<2
Pg/I
<2
Pg/I
3
EPA 200 7
2 pg/I
CHROMIUM
<5
Pg/I
<5
Pg/I
3
EPA 2003
5 Pg/I
COPPER
3
Pg/I
<2
Pg/I
3
EPA 200.7
2 Pg/I
LEAD
<10
Pg/I
<10
Pg/I
3
EPA 200.7
10 Pg/I
MERCURY
6.43
ng/I
4.06
ng/I
3
EPA 1631
1 ng/1
NICKEL
<10
Pg/I
<10
Pg/l
3
EPA 2003
10 Pg/I
SELENIUM
<10
Pg/I
<10
Pg/1
3
EPA 200.7
10 Pg/1
SILVER
<5
Pg/I
<5
Pg/I
3
EPA 2003
5 Pg/I
THALLIUM
<20
ug/I
<20
ug/I
3
EPA 200 7
20 ug/I
ZINC
257
Pg/I
104
Pg/I
3
EPA 200.7
10 Pg/I
CYANIDE
<5
Pg/I
<5
Pg/I
3
EPA 335A
5 pg/I
TOTAL PHENOLIC
COMPOUNDS
37
mg/I
15.6
mg/I
3
EPA 420 1
0.01 mg/I
HARDNESS (as CaCO3)
108
mg/I
90.7
mg/I
3
SM 2340 C
1 mg/I
Use this space (or a separate sheet) to provide information on other metals requested by the permit writer
T_
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 10 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
Outfall number: 001 [CY 2016 CY 20181 (Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
POLLUTANT
ANALYTICAL
ML/MDL
Number
Conc.
Units
Mass
Units
Conc.
Units
Mass
Units
of
METHOD
Samples
VOLATILE ORGANIC COMPOUNDS ['NOTE: different detection limits on samples]
ACROLEIN*
<50
-=
Pg/I
<50
Pg/I
3
EPA 624+AA
50 pg/I
ACRYLONITRILE*
<10
Pg/1
<10
Pg/I
3
EPA 624+AA
10 pg/I
BENZENE*
<1
Pg/I
<1
Pg/I
3
EPA 624
1 pg/I
BROMOFORM*
<1
Pg/1
<1
Pg/I
3
EPA 624
1 Pg/I
CARBON
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pg/I
TETRACHLORIDE*
CHLOROBENZENE*
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pg/I
CHLORODIBROMO-
<1
Pg/I
<1
Pg/I
3
EPA 624
1 1
METHANE*
CHLOROETHANE*
<5
Pg/I
<5
Pg/I
3
EPA 624
5 Pg/I
2-CHLOROETHYLVINYL
<5
Pg/I
<5
Pg/I
3
EPA 624
5 pg/l
ETHER*
CHLOROFORM*
<1
Pg/I
<1
Pg/I
3
EPA 624
1 1
DICHLOROBROMO-
<1
Pg/I
<1
Pg/I
3
EPA 624
1 pgll
METHANE*
1,1-DICHLOROETHANE*
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pg/I
1,2-DICHLOROETHANE*
<1
Pg/I
<1
Pg/l
3
EPA 624
1 Pg/I
TRANS-I,2-DICHLORO-
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pg/I
ETHYLENE*
1,1-DICHLORO-
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pg/I
ETHYLENE*
1,2-DICHLOROPROPANE*
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pgll
1,3-DICHLORO-
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pg11
PROPYLENE*
ETHYLBENZENE*
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pg/I
METHYL BROMIDE*
<5
Pg/I
<5
Pg/I
3
EPA 624
5 pg/l
METHYL CHLORIDE*
<5
Pg/l
<5
Pg/I
3
EPA 624
51
METHYLENE CHLORIDE*
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pg/I
1,1,2,2-TETRA-
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pg/I
CHLOROETHANE*
TETRACHLORO-
<1
Pg/I
<1
Pg/I
3
EPA 624
1 pg1l
ETHYLENE*
TOLUENE*
<1
Pg/I
<1
Pg/1
3
EPA 624
1 Pg/I
EPA Form 3510-2A (Rev. 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 11 of 22

FACILITY NAME AND PERMIT NUMBER:
Town of Wilkesboro Cub Creek, NCO021717
PERMIT ACTION REQUESTED:
2019 Renewal
RIVER BASIN:
Yadkin
Outfall number: 001 [CY 2016-20181 (Complete once for each outfall discharging effluent to waters of the United States.)
POLLUTANT
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
ANALYTICAL
METHOD
ML/MDL
Conc.
Units
Mass
Units
Conic.
Units
Mass
Units
Number
of
Samples
1,1,1
TRICHLOROETHANE'
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pgll
1,1,2
TRICHLOROETHANE`
<1
Pg/I
<1
Pg/I
3
EPA 624
1 Pgll
TRICHLOROETHYLENE"
<1
Pg/I
<1
pg/I
3
EPA 624
1 Pgll
VINYL CHLORIDE'
<5
Pg/I
<5
pg/I
3
EPA 624
5 pg/l
Use this space (or a separate sheet) to provide information on other volatile organic compounds requested by the permit writer
ACID -EXTRACTABLE COMPOUNDS
P-CHLORO-M-CRESOL
<10
Pg/I
<10
Pg/I
3
EPA 625
10 Pg/I
2-CHLOROPHENOL
<10
Pg/I
<10
Pg/I
3
EPA 625
10 Pg/I
2,4-DICHLOROPHENOL
<10
Pg/I
<10
pg/I
3
EPA 625
10 Pg/I
2,4-DIMETHYLPHENOL
<10
Pg/I
<10
Pg/I
3
EPA 625
10 pg/I
4,6-DINITRO-0-CRESOL
<50
Pg/I
<50
pg/I
3
EPA 625
50 pg/1
2,4-DINITROPHENOL
<50
Pg/I
<50
pg/I
3
EPA 625
50 pg/I
2-NITROPHENOL
<10
Pg/I
<10
Pg/I
3
EPA 625
10 Pg/I
4-NITROPHENOL
<50
Pg/I
<50
Pg/I
3
EPA 625
50 pg/I
PENTACHLOROPHENOL
<50
Pg/I
<50
Pg/I
3
EPA 625
50 Pg/I
PHENOL
<10
Pg/l
<10
Pg/l
3
EPA 625
10 Pg/I
TRIOHLOROPHENOL
RIC
<10
Pg/l
<10
pg/I
3
EPA 625
10 Pg/I
Use this space (or a separate sheet) to provide information on other acid -extractable compounds requested by the permit writer
BASE -NEUTRAL COMPOUNDS
ACENAPHTHENE
<10
Pg/I
<10
Pg/I
3
EPA 625
10 Pg/I
ACENAPHTHYLENE
<10
Pg/I
<10
Pg/I
3
EPA 625
10 Pg/I
ANTHRACENE
<10
Pg/I
<10
Pg/I
3
EPA 625
10 Pg/I
BENZIDINE
<50
Pg/I
<50
Pg/I
3
EPA 625
50 pg/I
BENZO(A)ANTHRACENE
<10
Pg/I
<10
Pg/I
3
EPA 625
10 Pg/I
BENZO(A)PYRENE
<10
Pg/I
<10
pg/I
3
EPA 625
10 Pg/I
EPA Form 3510-2A (Rev. 1-99) Replaces EPA forms 7550-6 & 7550-22. Page 12 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
Outfall number. 001 ICY 2016- CY 20181 (Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
POLLUTANT
ANALYTICAL
ML/MDL
Number
Conc.
Units
Mass
Units
Conc.
Units
Mass
Units
of
METHOD
Samples
3,4 BENZO-
<10
pg/l
<10
pg/I
3
EPA 625
10 pg/1
FLUORANTHENE
BENZO(GHI)PERYLENE
<10
pg/I
<10
pg/I
3
EPA 625
10 pg/I
BENZO(K)
<10
pg /I
<10
Pg/I
3
EPA 625
10 Pg/1
FLUORANTHENE
BIS (2-CHLOROETHOXY)
<10
Ng/I
<10
pg/I
3
EPA 625
10 Pg/I
METHANE
BIS (2-CHLOROETHYL)-
<10
pg/I
<10
Pg/I
3
EPA 625
10 pg/l
ETHER
BIS (2-CHLOROISO-
<10
pg /l
<10
pg/1
3
EPA 625
10 Pg/1
PROPYL)ETHER
BIS (2-ETHYLHEXYL)
<10
Ng/I
<10
Pg/1
3
EPA 625
10 Pg/I
PHTHALATE
4-BROMOPHENYL
<10
pg /I
<10
Pg/I
3
EPA 625
10 Pg/I
PHENYLETHER
BUTYL BENZYL
<10
Pg 11
<10
pg/I
3
EPA 625
10 pg/I
PHTHALATE
2-CHLORO-
<10
pg /I
<10
pg/I
3
EPA 625
10 Pg/I
NAPHTHALENE
4-CHLORPHENYL
<10
pg 11
<10
pgll
3
EPA 625
10 pg/I
PHENYLETHER
CHRYSENE
<10
Pg/I
<10
pg/I
3
EPA 625
10 pg/i
DI-N-BUTYL PHTHALATE
<10
pg/I
<10
Pg/I
3
EPA 625
10 Pg/I
DI-N-OCTYL PHTHALATE
<10
pg/I
<10
jig/1
3
EPA 625
10 pg/I
DIBENZO(A,H)
<10
jig/1
<10
Pgll
3
EPA 625
10 pgll
ANTHRACENE
1,2-DICHLOROBENZENE
<10
Pg/I
<10
pg/l
3
EPA 625
10pg/I
1,3-DICHLOROBENZENE
<10
Pg/l
<10
pg/I
3
EPA 625
10 pg/I
1,4-DICHLOROBENZENE
<10
pg/I
<10
Pg/I
3
EPA 625
10 pg/I
3,3-DICHLORO-
<50
pg /I
<50
Pgll
3
EPA 625
50 pg/1
BENZIDINE
DIETHYL PHTHALATE
<10
Pg/I
<10
Pg/I
3
EPA 625
10 Pg/I
DIMETHYL PHTHALATE
<10
pgll
<10
Pg/I
3
EPA 625
10 Pg/I
2,4-DINITROTOLUENE
<10
Pg/l
<10
pg/I
3
EPA 625
10 Pg/I
2,6-DINITROTOLUENE
<10
Pgll
<10
Pg/I
3
EPA 625
10 Pgll
1,2-DIPHENYL-
<10
pg /l
<10
Pg/l
3
EPA 625
10 Pg/I
HYDRAZINE
EPA Form 3510-2A (Rev. 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 13 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
Outfall number: 001 ICY -CY XXX] (Complete once for each outfall discharging effluent to waters of the United States )
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
POLLUTANT
ANALYTICAL
ML/MDL
Number
Conc.
Units
Mass
Units
Conc.
Units
Mass
Units
of
METHOD
Samples
FLUORANTHENE
<10
pg/I
<10
Ng/I
3
EPA 625
10 pg/I
FLUORENE
<10
pg/I
<10
Ng/I
3
EPA 625
10 Ng/I
HEXACHLOROBENZENE
<10
Ng/I
<10
pg/I
3
EPA 625
10 Ng/I
HEXACHLORO-
<10
Ng/I
<10
Ng/I
3
EPA 625
10 pg/I
BUTADIENE
HEXACHLOROCYCLO-
<50
Ng/I
<50
Ng/I
3
EPA 625
50 j l
PENTADIENE
HEXACHLOROETHANE
<10
Ng/I
<10
Pg/I
3
EPA 625
10 Ng/I
INDENO(1,2,3-CD)
<10
Ng/I
<10
pg/I
3
EPA 625
10 pg/l
PYRENE
ISOPHORONE
<10
Ng/I
<10
Ng/l
3
EPA 625
10 Ng/l
NAPHTHALENE
<10
Ngll
<10
Ng/I
3
EPA 625
10 Ng/I
NITROBENZENE
<10
Ng/I
<10
Ng/I
3
EPA 625
10 Ng/I
N-NITROSODI-N-
<10
Ng/I
<10
Ng/I
3
EPA 625
10 Ng/I
PROPYLAMINE
N-NITROSODI-
<10
Ng/I
<10
Ng/I
3
EPA 625
10 Ng/I
METHYLAMINE
N-NITROSODI-
<10
Ng/I
<10
pg/I
3
EPA 625
10 pg/I
PHENYLAMINE
PHENANTHRENE
<10
Ng/I
<10
Ng/I
3
EPA 625
10 Ng/I
PYRENE
<10
pg/I
<10
Ng/I
3
EPA 625
10 pg/I
1,2,4
TRICHLOROBENZENE
<10
Ng/I
<10
Ng/I
3
EPA 625
10 pg/I
Use this space (or a separate sheet) to provide information on other base -neutral compounds requested by the permit writer
Use this space (or a separate sheet) to provide information on other pollutants (e g., pesticides) requested by the permit writer
END OF PART D.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
EPA Form 3510-2A (Rev 1-99). Replaces EPA forms 7550-6 & 7550-22 Page 14 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
SUPPLEMENTAL APPLICATION INFORMATION
PART E. TOXICITY TESTING DATA
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points: 1) POTWs with a design flow rate greater than or equal to 1.0 mgd, 2) POTWs with a pretreatment program (or those that are
required to have one under 40 CFR Part 403). or 3) POTWs required by the permitting authority to submit data for these parameters.
• At a minimum, these results must include quarterly testing for a 12-month period within the past 1 year using multiple species (minimum of two
species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results
show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation, if one was conducted.
• If you have already submitted any of the information requested in Part E, you need not submit it again Rather, provide the information
requested in question EA for previously submitted information. If EPA methods were not used, report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E.
If no biomonitoring data is required, do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
complete.
E.1. Required Tests.
Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years. [2014-2018: XX daphnia and X fat head minnow]
FJ chronic ❑ acute [SEE ATTACHED WET TEST SUMMARY- PART E ATTACHMENTS 1 & 2]
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years Allow one
column per test (where each species constitutes a test). Copy this page if more than three tests are being reported.
Test number: Test number: Test number:
a. Test information.
Test Species & test method number
Age at initiation of test
Outfall number
Dates sample collected
Date test started
Duration
b. Give toxicity test methods followed
Manual title
Edition number and year of publication
Page number(s)
c. Give the sample collection method(s) used For multiple grab samples, indicate the number of grab samples used.
24-Hour composite
Grab
d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection
After dechlorination
EPA Form 3510-2A (Rev. 1-99) Replaces EPA forms 7550-6 & 7550-22. Page 15 of 22

FACILITY NAME AND PERMIT NUMBER:
Town of Wilkesboro Cub Creek, NCO021717
PERMIT ACTION REQUESTED:
2019 Renewal
RIVER BASIN:
Yadkin
Test number: 1 Test number: 2 Test number:
e. Describe the point in the treatment process at which the sample was collected
Sample was collected:
f. For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both
Chronic toxicity
Acute toxicity
g_ Provide the type of test performed.
Static
Static -renewal
Flow -through
h. Source of dilution water. If laboratory water, specify type, if receiving water, specify source
Laboratory water
Receiving water
i. Type of dilution water. If salt water, specify "natural' or type of artificial sea salts or brine used
Fresh water
Salt water
j- Give the percentage effluent used for all concentrations in the test series.
k Parameters measured during the test. (State whether parameter meets test method specifications)
pH
Salinity
Temperature
Ammonia
Dissolved oxygen
I. Test Results.
Acute:
Percent survival in 100%
effluent
%
%
°
�0
LC5o
95% C.I.
%
%
%
Control percent survival
%
%
%
Other (describe)
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22 Page 16 of 22

FACILITY NAME AND PERMIT NUMBER:
Town of Wilkesboro Cub Creek, NCO021717
PERMIT ACTION REQUESTED:
2019 Renewal
RIVER BASIN:
Yadkin
Chronic:
NOEC
%
%
%
IC25
%
%
%
Control percent survival
%
%
%
Other (describe)
m. Quality Control/Quality Assurance_
Is reference toxicant data available?
Was reference toxicant test within
acceptable bounds?
What date was reference toxicant test
run (MM/DD/YYYY)?
Other (describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ❑ No If yes, describe:
E.4. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information, or information regarding the
cause of toxicity, within the past four and one-half years, provide the dates the information was submitted to the permitting authority and a summary
of the results.
Date submitted: / /_ (MM/DD/YYYY)
Summary of results: (see instructions)
WET Test Summary Included and Labeled "PART E ATTACHMENTS 1 & 2". NPDES Permit NCO021717 requires quarterly chronic
WET analyses on the final effluent after disinfection in March June September December and data submittal quarterly on corresponding
eDMRs and to the DEQ Environmental Sciences Branch Four second species tests are also included per NPDES permit requirement
WET 2014-2018 = XX Chronic Daphnia tests: XX passed/X invalid /X failed: X fat head minnow tests: X passed. XXX FAILS?)
END OF PART E.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22. Page 17 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
SUPPLEMENTAL APPLICATION INFORMATION
PART F. INDUSTRIAL USER DISCHARGES AND RCRA/CERCLA WASTES
All treatment works receiving discharges from significant industrial users or which receive RCRA,CERCLA, or other remedial wastes must
complete part F.
GENERAL INFORMATION:
F.1. Pretreatment program. Does the treatment works have, or is subject to, an approved pretreatment program?
Yes ❑ No
F. 2. Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works. TOTAL OF 2 SIGNIFICANT INDUSTRIAL USERS
a. Number of non -categorical SIUs. 2
b. Number of CIUs. 0
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU. If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU. SIU SUMMARY FORMS ARE INCLUDED AND LABELED PART F ATTACHMENTS 1-2
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary
Name: Wilkes County Landfill (Lechate Only)
Mailing Address: 110 North St.
Wilkesboro, NC 28697
FA. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
Lechate from pond. Aeration in pond (see attached)
F.5. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge
Principal product(s): Lechate only from landfill
Raw material(s):
F.6. Flow Rate.
a. Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent.
49 494 gpd ( continuous or X intermittent)
b. Non -process wastewater flow rate. Indicate the average daily volume of non -process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent
gpd (_ continuous or intermittent)
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following:
a. Local limits Yes ❑ No
b. Categorical pretreatment standards ❑ Yes ® No
If subject to categorical pretreatment standards, which category and subcategory?
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 18 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
F.8. Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e g
upsets, interference) at the treatment works in the past three years?
�7 Yes ® No If yes, describe each episode.
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE:
F.9. RCRA Waste. Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe?
❑ Yes ® No (go to F.12)
F.10. Waste transport. Method by which RCRA waste is received (check all that apply):
❑ Truck ❑ Rail ❑ Dedicated Pipe
F.11. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units)
EPA Hazardous Waste Number Amount Units
CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
FA 2. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
❑ Yes (complete F.13 through F 15 ) ® No [None from CERCLA or RCRA and none that meets the SIU definition]
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is expected to originate in
the next five years).
F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received) Include data on volume and concentration, if
known. (Attach additional sheets if necessary )
F.15. Waste Treatment.
a. Is this waste treated (or will be treated) prior to entering the treatment works?
❑ Yes ❑ No
If yes, describe the treatment (provide information about the removal efficiency):
b. Is the discharge (or will the discharge be) continuous or intermittent?
❑ Continuous ❑ Intermittent If intermittent, describe discharge schedule.
END OF PART F.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 19 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
SUPPLEMENTAL APPLICATION INFORMATION
PART G. COMBINED SEWER SYSTEMS
If the treatment works has a combined sewer system, complete Part G. NOT APPLICABLE
G.I. System Map. Provide a map indicating the following: (may be included with Basic Application Information)
a. All CSO discharge points.
b. Sensitive use areas potentially affected by CSOs (e.g., beaches, drinking water supplies, shellfish beds, sensitive aquatic ecosystems, and
outstanding natural resource waters).
c Waters that support threatened and endangered species potentially affected by CSOs
G.2. System Diagram. Provide a diagram, either in the map provided in G.1 or on a separate drawing, of the combined sewer collection system that
includes the following information.
a. Location of major sewer trunk lines, both combined and separate sanitary.
b Locations of points where separate sanitary sewers feed into the combined sewer system
C. Locations of in -line and off-line storage structures.
d. Locations of flow -regulating devices.
e Locations of pump stations.
CSO OUTFALLS:
Complete questions G.3 through G.6 once for each CSO discharge point.
G.3. Description of Outfall.
a Outfall number
b. Location
(City or town, if applicable) (Zip Code)
(County) (State)
(Latitude) (Longitude)
c- Distance from shore (if applicable) ft
d Depth below surface (if applicable)
e Which of the following were monitored during the last year for this CSO?
❑ Rainfall ❑ CSO pollutant concentrations ❑ CSO frequency
❑ CSO flow volume ❑ Receiving water quality
f How many storm events were monitored during the last year?
GA. CSO Events.
a Give the number of CSO events in the last year
_ events (`j actual or ❑ approx.)
b Give the average duration per CSO event
_ hours (❑ actual or ❑ approx.)
EPA Form 3510-2A (Rev, 1-99) Replaces EPA forms 7550-6 & 7550-22. Page 20 of 22

IUP, PART I,
Industrial User (IU) Specific Conditions
E.) Schematic and Monitoring Locations:
The facility schematic and description of monitoring location(s) given below must
show enough detail such that someone unfamiliar with the facility could readily find
and identify the monitoring location(s) and connection to the sewer. Include and
identify all regulated pipes.
Leachate Re -Circulation Diagram
50' to 100' so;iker hose
7j,p
PVC Ads-pter
1" PVC

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717 2019 Renewal Yadkin
C. Give the average volume per CSO event.
million gallons (❑ actual or ❑ approx )
d. Give the minimum rainfall that caused a CSO event in the last year
Inches of rainfall
G.5. Description of Receiving Waters.
a. Name of receiving water:
b. Name of watershed/river/stream system:
United State Soil Conservation Service 14-digit watershed code (if known):
c. Name of State Management/River Basin:
United States Geological Survey 8-digit hydrologic cataloging unit code (if known):
G.6. CSO Operations.
Describe any known water quality impacts on the receiving water caused by this CSO (e.g., permanent or intermittent beach closings, permanent or
intermittent shell fish bed closings, fish kills, fish advisories, other recreational loss, or violation of any applicable State water quality standard).
END OF PART G.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 21 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
SUPPLEMENTAL APPLICATION INFORMATION
PART F. INDUSTRIAL USER DISCHARGES AND RCRA/CERCLA WASTES
All treatment works receiving discharges from significant industrial users or which receive RCRA,CERCLA, or other remedial wastes must
complete part F.
GENERAL INFORMATION:
F.1. Pretreatment program. Does the treatment works have, or is subject to, an approved pretreatment program?
Yes [l No
F.2. Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works. TOTAL OF 2 SIGNIFICANT INDUSTRIAL USERS
C. Number of non -categorical SIUs.
d. Number of CIUs J
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU. If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU. SIU SUMMARY FORMS ARE INCLUDED AND LABELED PART F ATTACHMENTS 1-2
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
Name: Tyson Foods, Inc
Mailing Address: P.O Box 88
Wilkesboro, NC 28697
FA. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
See attachments
F.5. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
Principal product(s): Chicken products
Raw material(s): Chickens
F.6. Flow Rate.
C. Process wastewater flow rate Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent.
2.6767,000 gpd ( , ,_ _ continuous or intermittent)
d. Non -process wastewater flow rate Indicate the average daily volume of non -process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent.
gpd ( continuous or intermittent)
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following:
a. Local limits ❑ Yes ❑ No
b. Categorical pretreatment standards ❑ Yes ❑ No
If subject to categorical pretreatment standards, which category and subcategory?
NPDES FORM 2A Additional Information

Sheetl
Flow Chart Waste Water, Permit Required Confined Spaces
Page 1

Sheets
EIf
Confine Space
Permit Required
LoCkNolve
Confine
Permit F
Tyson Lift
PUMP Station at
city
(3} T,10 TS HP Pumps
Confine Space
Permit Required
Valve
Con me Space
Permit Required
basin
Page 2

Sheetl
Wavlty flow
25hp ;eivnp
valve ND
Mixer
60hp
vary
Basin
7 IMG
Basin 7.5/mg Ohp
INC'
{ �e
_
t xer M
25hp
Mixer
Bypass _.__� 1--Pypass
Vaity
Aerators
25hp
Valve NC Mixer
`10hp
10hp .
Basin
7.5/MG
V N. NC
�,,,,—, 25hp
�=1 Ohn Mixer
Soho
Note Valve Symbols NC Normally Close
NO Normally Open
' NC
alv
Eft to Town of Wilkesboro P.O.T.W
fl
Eff.san4ft point& Towns lab
spout
1�
Effluent
Page 3

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Town of Wilkesboro Cub Creek, NCO021717
2019 Renewal
Yadkin
F.B. Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e.g.,
upsets, interference) at the treatment works in the past three years?
❑ Yes r1j No If yes, describe each episode.
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE:
F.9. RCRA Waste. Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe?
❑ Yes ® No (go to F.12)
F.10. Waste transport. Method by which RCRA waste is received (check all that apply):
❑ Truck E Rail ❑ Dedicated Pipe
F.11. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units).
EPA Hazardous Waste Number Amount Units
CERCLA (SUPERFUND) WASTEWATER, RCRA REM EDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
F.12. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
Yes (complete F.13 through F.15.) ® No [None from CERCLA or RCRA and none that meets the SIU definition]
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is expected to originate in
the next five years).
F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received) Include data on volume and concentration, if
known (Attach additional sheets if necessary )
F.15. Waste Treatment.
a. Is this waste treated (or will be treated) prior to entering the treatment works?
❑ Yes ❑ No
If yes, describe the treatment (provide information about the removal efficiency):
b. Is the discharge (or will the discharge be) continuous or intermittent?
i
❑ Continuous El Intermittent If intermittent, describe discharge schedule.
END OF PART F.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
NPDES FORM 2A Additional Information

October 91h, 2018
Mr. Jim Gonsiewski
Facility Name: Town of Wilkesboro
Permit Number: WQ0000092
Period: January 1, 2017- December 31, 2017
Dear Mr. Gonsiewski,
The Town of Wilkesboro has a contract with the County of Wilkes to dispose of the
POTW's Wastewater Treatment Biosolids in Wilkes County's (Subtitle D) Landfill located
at Roaring River, North Carolina.
A total of 4,355.60 tons was hauled from the Wilkesboro's Cub Creek Wastewater
Treatment Plant during 2017.
Ken Noland
Town Manager

CITY OF WILKESBORO POTW NCO021717 SECOND SPECIES BIOASSAY [Fathead Minnow]
March 22,24,25 2016 1 (with daphnia and #1 PPA-#1 Fish)
Repl.
1
2
3
4
Control
Surviving #
10
10
10
10
% Survival
100
Results
Original #
10
10
10
10
Survival
Growth
WgUoriginal (mg)
0.610
0.794
0.692
0.605
Avg Wgt (mg)
0.675
NOEC
14.8
14.8
LOEC
>14.8
>14.8
0.92% Eff
Surviving #
10
9
9
9
% Survival
92.5
ChV
>14.8
>14.8
Original #
10
10
10
10
WgUoriginal (mg)
0.692
0.515
0.593
0.522
Avg Wgt (mg)
0.581
Overall
ChV
>14.8
1.85% Eff
Surviving #
10
10
10
10
% Survival
100
Original #
10
10
10
10
WgUoriginal (mg)
0.693
0.661
0.686
0.71
Avg Wgt (mg)
0.688
3.7% Eff
Surviving #
9
10
9
10
% Survival
95
Original #
10
10
10
10
Wgt/original (mg)
0.694
0.698
0.625
0.674
Avg Wgt (mg)
0.673
7.4% Eff
Surviving #
10
10
10
9
% Survival
97.5
Original #
10
10
10
10
WgUoriginal (mg)
0.783
0.572
0.555
0.739
Avg Wgt (mg)
0.662
14.8% Eff
Surviving #
10
10
10
8
% Survival
95
Original #
10
10
10
10
WgUoriginal (mg)
0.664
0.756
0.806
0.69
Avg Wgt (mg)
0.729
(with daphnia and #2 PPA-#2 Fish)
1 2 3 4
JUNE 7/9/10, 2016
Repl.
Control
Surviving #
10
10
10
10
% Survival
100
Results
Original #
10
10
10
10
Survival
Growth
WgUoriginal (mg)
0.281
0.294
0.433
0.338
Avg Wgt (mg)
0.337
NOEC
14.8
14.8
LOEC
>14.8
>14.8
0.92% Eff
Surviving #
10
10
10
10
% Survival
100
ChV
>14.8
>14.8

Original #
10
10
10
10
WgUoriginal (mg)
0.433
0.515
0.279
0.434
Avg Wgt (mg)
0.415
Overall
ChV
>14.8
1.85% Eff
Surviving #
10
10
10
9
% Survival
97.5
Original #
10
10
10
10
WgUoriginal (mg)
0.456
0.358
0.475
0.408
Avg Wgt (mg)
0A24
3.7% Eff
Surviving #
10
9
10
10
% Survival
97.5
Original #
10
10
10
10
WgUoriginal (mg)
0.354
0.406
0.419
0.424
Avg Wgt (mg)
0.401
7.4% Eff
Surviving #
9
9
10
10
% Survival
95
Original #
10
10
10
10
WgUoriginal (mg)
0.378
0.351
0.395
0.386
Avg Wgt (mg)
0.378
14.8% Eff
Surviving #
10
10
10
10
% Survival
100
Original #
10
10
10
10
WgUoriginal (mg)
0.496
0.526
0.393
0.484
1 Avg Wqt (mq)
1 0.475
SEPTEMBER 13/15/16, 2016 (with daphnia and no PPA-#3 Fish)
Reol. 1 1 1 2 1 3 1 4
Control
Surviving #
10
9
10
10
% Survival
97.5
Results
Original #
10
10
10
10
Survival
Growth
WgUoriginal (mg)
0.648
0.733
0.649
0.776
Avg Wgt (mg)
0.702
NOEC
14.8
14.8
LOEC
>14.8
>14.8
0.92% Eff
Surviving #
9
10
9
10
% Survival
95
ChV
>14.8
>14.8
Original #
10
10
10
10
WgUoriginal (mg)
0.449
0.556
0.53
0.638
Avg Wgt (mg)
0.543
Overall
ChV
>14.8
1.85% Eff
Surviving #
10
10
10
10
% Survival
100
Original #
10
10
10
10
WgUoriginal (mg)
0.639
0.66
0.735
0.825
Avg Wgt (mg)
0.715
3.7% Eff
Surviving #
10
10
10
10
% Survival
1 00

Original #
10
10
10
10
Wgt/original (mg)
0.698
0.683
0.739
0.823
Avg Wgt (mg)
0.736
7.4% Eff
Surviving #
10
10
10
10
% Survival
100
Original #
10
10
10
10
WgUoriginal (mg)
0.738
0.664
0.608
0.715
Avg Wgt (mg)
0.681
14.8% Eff
Surviving #
10
10
10
10
% Survival
100
Original #
10
10
10
10
Wgt/original (mg)
0.693
0.739
0.758
0.736
Avg Wgt (mg)
0.732
DECEMBER 6/8/9, 2016 (with daphnia and #3 PPA-94 Fish)
Repl. 1 2 3 4
Control
Surviving #
10
9
10
10
% Survival
97.5
Results
Original #
10
10
10
10
Survival
Growth
Wgt/original (mg)
0.77
0.706
0.79
0.692
Avg Wgt (mg)
0.740
NOEC
14.8
14.8
LOEC
>14.8
>14.8
0.92% Eff
Surviving #
9
10
9
10
% Survival
95
ChV
>14.8
>14.8
Original #
10
10
10
10
WgUoriginal (mg)
0.627
0.779
0.801
0.696
Avg Wgt (mg)
0.726
Overall
ChV
1.85% Eff
Surviving #
10
10
10
10
% Survival
100
Original #
10
10
10
10
Wgt/original (mg)
0.695
0.779
0.801
0.696
Avg Wgt (mg)
0.743
3.7% Eff
Surviving #
9
10
10
10
% Survival
97.5
Original #
10
10
10
10
Wgt/original (mg)
0.88
0.653
0.834
0.772
Avg Wgt (mg)
0.785
7.4% Eff
Surviving #
10
10
10
9
% Survival
97.5
Original #
10
10
10
10
Wgt/original (mg)
0.88
0.653
0.834
0.831
Avg Wgt (mg)
0.800
14.8% Eff
Surviving #
10
10
10
10
% Survival
100

Original # 10 10 10 10
WgUoriginal (mg) 0.951 0.84 0.878 0.831 Avg Wgt (mg) 0.875
OCTOBER 2/4/5, 2018 (with daphnia and #3 PPA-#4 Fish)
ReW. I 1 1 2 1 3 1 4
Control
Surviving #
10
9
1
9
% Survival
72.5
Results
Original #
10
10
10
10
Survival
Growth
WgUoriginal (mg)
0.970
0.784
0.853
0.849
Avg Wgt (mg)
0.864
NOEC
14.8
14.8
LOEC
>14.8
>14.8
0.92% Eff
Surviving #
9
9
9
9
% Survival
90.0
ChV
>14.8
>14.8
Original #
10
10
10
10
WgUoriginal (mg)
0.894
0.647
0.83
0.619
Avg Wgt (mg)
0.748
Overall
ChV
>14.8
1.85% Eff
Surviving #
10
9
9
9
% Survival
92.5
Original #
10
10
10
10
WgUoriginal (mg)
0.796
0.714
0.698
0.743
Avg Wgt (mg)
0.738
3.7% Eff
Surviving #
10
10
10
10
% Survival
100
Original #
10
10
10
10
WgUoriginal (mg)
0.729
0.706
0.74
0.783
Avg Wgt (mg)
0.740
7.4% Eff
Surviving #
8
9
10
8
% Survival
87.5
Original #
10
10
10
10
WgUoriginal (mg)
0.702
0.736
0.912
0.789
Avg Wgt (mg)
0.785
14.8% Eff
Surviving #
10
10
10
10
% Survival
100
Original #
10
10
10
10
WgUoriginal (mg)
0.771
0.812
0.755
0.756
Avg Wgt (mg)
1 0.774
DECEMBER 416/7, 2018 (with daphnia and #3 PPA-#4 Fish)
Repl. 1 1 2 1 3 1 4

Control
Surviving #
10
10
10
10
% Survival
100
Results
Original #
10
10
10
10
Survival
Growth
WgUoriginal (mg)
0.592
0.597
0.618
0.602
Avg Wgt (mg)
0.602
NOEC
14.8
14.8
LOEC
>14.8
>14.8
0.92% Eff
Surviving #
10
9
10
10
% Survival
97.5
ChV
>14.8
>14.8
Original #
10
10
10
10
WgUoriginal (mg)
0.513
0.527
0.594
0.674
Avg Wgt (mg)
0.577
Overall
ChV
>14.8
1.85% Eff
Surviving #
10
10
10
10
% Survival
100
Original #
10
10
10
10
WgUoriginal (mg)
0.692
0.527
0.640
0.570
Avg Wgt (mg)
0.607
3.7% Eff
Surviving #
9
10
10
10
% Survival
97.5
Original #
10
10
10
10
WgUoriginal (mg)
0.369
0.456
0.601
0.592
Avg Wgt (mg)
0.505
7.4% Eff
Surviving #
10
9
10
10
% Survival
97.5
Original #
10
10
10
10
WgUoriginal (mg)
0.565
0.522
0.55
0.436
Avg Wgt (mg)
0.518
14.8% Eff
Surviving #
10
10
10
10
% Survival
100
Original #
10
10
10
10
WgUoriginal (mg)
0.591
0.704
0.693
0.717
Avg Wgt (mg)
0.676

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:3/27/2019
Facility: Wilkesboro NPDES # NC00 21717 Pipe #: 001 County: Wilkes
La ory: Meritec Inc. Comments
x
Si ature of Operator in Responsible Charge
x
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Water Sciences Section
Aquatic Toxicology Branch
Division of Water Resources
1621 Mail Service Center
Raleigh, N.C. 27699-1621
Test Initiation Date/Time 6/7/2016 4:15 PM Avg Wt/Surv. Control 0.337
% Eff. Repl. 1 2 3 4
Control 1 Surviving #
Original #
Wt/original (mg)
0.92 Surviving #
Original #
Wt/original (mg)
1.85 Surviving #
Original #
Wt/original (mg)
3.7 Surviving #
Original #
Wt/original (mg)
10
10
10
10
10
10
10
10
0.433
0.515
0.279
0.434
10
10
10
9
10
10
10
10
0.456
0.358
0.475
0.408
% Survival 100.0
Test Organisms
_ Cultured In -House
Outside Supplier
Avg Wt (mg) 0.337 Hatch Date: 6/6/16
% Survival 100.0 Hatch Time: 3 - 4 pm CST
Avg Wt (mg) 0.415
% Survival 97.5
Avg Wt (mg) 0.424
% Survival 97.5
Avg Wt (mg) 0 401
7.4 Surviving #
% Survival 95.0
Original #
Wt/original (mg)
Avg Wt (mg) 0.378
14.8 Surviving #
% Survival 100.0
Original #
Wt/original (mg)
Avg Wt (mg) 0.475
Water Quality Data
Day
Control
0
1
2
3
4 5
6
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
0
1
2
3
4 5
6
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
1
2
3
Survival Growth
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp. at Receipt (°C)
8.13
/ 7.74
8.02
/ 7 69
8.10
/ 7.80
8.15
/ 8.05
8.11
/ 8.03
8.18
/ 7.85
8.06
/ 7.88
7.78
/ 7.07
7.89
! 6.89
7.80
! 6.85
7.84
/ 7.09
7.79
/ 7.56
7.93
17.60
8.01
/ 7.53
24.3
/ 24.7
24.2
/ 24.6
24.2
/ 24.3
24.2
/ 24.9
24.6
/ 24.9
24.4
/ 25-0
24.0
/ 24.5
8.02
/ 7 97
8.03
/ 7.76
7.97
/ 7.86
8 07
/ 7.81
7.99
/ 8.09
8.14
/ 7.96
8.12
/ 8.07
7.68
/ 7.38
7.87
/ 6J3
7.72
/ 6.68
7.76
/ 7.29
7.69
! 7.50
7.84
/ 7.47
8.02
! 7 83
24.5
/ 25.0
25 0
/ 24.7
25.2
/ 25.5
25.1
/ 25.2
24.8
/ 24.5
24.2
/ 25.6
24.3
/ 25.1
6/7/2016
6/8/2016
6/9/2016
20.8
24.0
25.8
88
90
94
162
153
153
1002
1087
1108
<0.1
<0.1
<0.1
0.7
1.8
1.4
Dilution H2O Batch # 1026 1027 1028 F40
Hardness (mg/L) 42 44 42 Alkalinity (mg/L)49 53 51 Conductivity (umhos/cm) 172 215 203
Normal
Hom. Var.
NOEC
14.8
14.8
LOEC
>14.8
>14.8
ChV
>14.8
>14.8
Method
Steel's
Dunnett's
Overall Result
ChV >14.8
Stats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
0.92
10 18
10 16
2.41-1.8443
2.41-2.0551
1.85
3.7
10 16
10 14
2.41-1.5047
2.41-0.9602
7.4
H14.77 8
10 18
2.41-3.2378
DWQ Form AT-5

• Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:3/31/2016
Facility: Wilkesboro
NPDES # NC00 21717
Pipe #: 001 County: Wilkes
Inc.
Sig ature of Operator in Respo ble Ch
x , �) C_-4f�
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Water Sciences Section
Aquatic Toxicology Branch
Division of Water Resources
1621 Mail Service Center
Raleigh, N.C. 27699-1621
Test Initiation Date/Time 3/22/2016 3:30 PM Avg Wt/Surv. Control 0.675
% Eff. Repl. 1 2 3 4
FC-o-n-t-ro-11 Surviving #
Original #
Wt/original (mg)
0.92 Surviving #
Original #
Wt/original (mg)
10
10
10
10
10
10
10
10
0.610
0.794
0.692
0.605
10
9
9
9
10
10
10
10
0.692
0.515
0.593
0.522
% Survival 100.0
Avg Wt (m
g).F 0.675
Test Organisms
7 Cultured In -House
f7 Outside Supplier
Hatch Date: 3/21/16
% Survival 9-2-51 Hatch Time: 3:00 pm CT
Avg Wt (mg) 0.581
F--18-5-1 Surviving #
% Survival 100.0
Original #
Wt/original (mg)
Avg Wt (mg) 0.688
3.7 Surviving #
% Survival 95.0
Original #
Wt/original (mg)
Avg Wt (mg) 0.673
7.4 Surviving #
% Survival 97.5
Original #
Wt/original (mg)Avg
Wt (mg) 0.662
10
10
10
9
10
10
10
1
14.87 Surviving #
10
10
10
8
% Survival
95.0
Original #
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp. at Receipt (°C)
8.35
! 7.82
8.08
7.87
8.26
/ 8.01
8.19
/ 8.07
8.20
/ 7.84
8.19
17.89
8.11
17.89
0 1 2 3 4 5 6
8.16
/ 7.94
8.10
/ 7.91
8.04
/ 7.87
8.02
17.96
8.08
/ 7.83
8.08
/ 8.02
8.13
/ 7.88
7.71
/ 7.07
7.65
/ 7.10
7.74
/ 7.12
7.75
/ 7.48
7.76
/ 6.83
7.82
/ 7.20
7.83
/ 6.41
24.8
/ 24.3
25.5
/ 24.3
24.5
/ 24.2
24.9
/ 24.2
24.3
/ 24.7
24.4
/ 24.7
25.0
/ 24.6
1 2 3
3/21 /2016
3/23/2016
3/24/2016
21.4hrs
24.Ohrs
26.4hrs
316
272
256
77
72
86
1072
1183
1155
<0.1
<0.1
<0.1
0.6
0.5
0.5
Dilution H2O Batch # 993 994 995 996 997 998
Hardness (mg/L) 44 46 46 46 42 46
Alkalinity (mg/L) 56 55 56 55 55 54
Conductivity (umhos/cm) 226 244 210 198 196 207
Survival
Growth
Normal
Yes
F1
Hom. Var.
Irl
11*711
NOEC
14.8
14.8
LOEC
>14.8
>14.8
ChV
>14.8
>14.8
Method
Steel's
Dunnett's
Overall Result
ChV >14.8
Stats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
0.92
10 12
2.41 1.7887
1.85
10 18
2.41-0.2313
3.7
10 14
2.41 0.0472
7.4
10 16
2.41 0.2454
14.8
10 16
2.41-1.0147
10
10
10
10
9
10
9
10
10
10
10
10
0.694
0.698
0.625
0.674

I
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Client: Wilkesboro # of Organisms per Chamber: 10 Start Date: 3/22/16
NPDES #: NCOO21717 Test Vessel Size: 400 ml End Date: 3/29/16
Initiated by: Test Solution Volume: 250 ml
Time:
Time: ;
Date/Time Fed:
3/22/16 '1 a ,C ,.
Temp. of Stock:,`-1
�, °C Randomization: Yes No
Date/Time
Born:
3/21/16 3:00 PM CT
# of Reps:
4
Incubator #: t .
Organism Source:
Aquatox, Inc.
Analyst(s):
MR, TH,
CD
Transferred by:
Fed by:
Date
Time Initials
Date
Time 1 --- Initials Time 2 Initials
Day 1
3/23
r1
Day 0
3/22
-
Day 2
3/24
�j `` �" f
Day 1
3/23
n'
�A, ,
Day 3
3/25
, (' . ��
Day 2
3/24
Day 4
3/26
Day 3
3/25
`� l� M /�L- �'L\M �►�,�
Days
3/27
J r- Nv
Day
3/26
.C%.�,� C_
Day 6
3/28
(ll'�1 �'
Day
3/28
Test Termination Data:
Initials/Signature:
End Date: 3/29/16
End Time

AML
ENVIRONMENTAL LABORATORIESADivision of Water Techr,c>1c>gy and Controls, lnc.
Chemical and Physical Determinations
Client: Wilkesboro NPDES#: NCO021717 Start Date: 3/22/16
Test Organism: Pimephales promelas
Day
End Date: 3/29/16
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Freshwater
Batch #
e-
pH: Initial
T, 7j
0,
'S
Final
7gq
D.O. Initial
Z
7
Final
c<:,
C ,,,tS 1
7 c l
Temp.: Initial
Z (2,
24, k
Final
Conductivity: Initial
Final
7
Residual Chlorine:
-'-0
I'
Hardness:
Alkalinity:,
45
Day
Concentration: 0.92%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Kemarks
pH: Initial
ks
Final
t
7�
7,
S
1--) ,n
7 60
D.O. Initial
-7
Final
1c,
Temp.: Initial
Final
Conductivity: Initial
Final
Residual Chlorine:
Day
Concentration: 1.85%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
Final
D.O.: Initial
17
7
Final
Temp.: Initial
Final
Conductivity: Initial
Final
�' o
�
Residual Chlorine: 1,:f-&
C(-,.
--SC

Client: Wilkesboro
ENVIRONMENTAL LA BO RATOfilES
Chemical and Physical Determinations
NPDES#: NCO021717
Test Organism: Pimephales promelas
Day
Start Date: 3/22/16
End Date: 3/29/16
Page 2 of 2
Concentration: 3.7%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
",
"
, - 41,
(,
7=1
:",//
Final
A
7, (IN
Ll
D.O.: Initial
.71
•7
arc
'74
Final
j'
L� 77
Temp.: Initial
>J11
2 'i
Li
Final
-2
2 4, '7
-
7,1A
)M T
Conductivity: Initial
Final
Li
G C,
Residual Chlorine:
4:-C, I
Ln
Z
Day
Concentration: 7.4%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
k ci
Final
TT
N
D.O.: Initial
7_ Z
Final
c:,
C 4>'
7./
Temp.: Initial
'I �
, I Ll
Final
--,5
Conductivity: Initial
Final
L
1
-j!
2
-2 1
-3
Residual Chlorine:
t
0 k
-7
4,
-
1-2'
E,1
4L
Day
Concentration: 14.8%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
<3�11 I &
Final
Cj _A
"1 C
-7
D.O.: Initial
77)-
Final
1
1 CU
7. l
-7,
Temp.: Initial
Li
Final
14
Conductivity: Initial
Final
Residual Chlorine:
Day
Concentration: 100%
0-1
1-2
2-3
3-4
4-5
5-6
0-7
Remarks
-1
pH:
Conductivity:
Residual Chlorine:
Hardness;
Alkalinity:

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Wilkesboro NPDES#: NC0021717
Test Organism: Pimephales promelas
Start Date: 3/22/16
End Date: 3/29/16
Concentration
Control
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
��
Cl
Rep B
0
Rep C
0
Rep D
0�-
Concentration
0.92%
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0�
Rep B
0
jT
Rep C
0'
Rep D
0
Concentration
1.85%
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
Rep B
0
Rep C
0
5
Rep D
0
-
-

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client:
Wilkesboro
NPDES#:
NCO021717 Start Date:
3/22/16
Test Organism:
Pimephales
promelas
End Date:
3/29/16
Concentration
3.7%
Tuesday
Wednesday
Thursday
Friday Saturday Sunday
Monday Tuesday
Rep A
0
/
Rep B
0�_-
Rep C
0
-
T
/.
�,/
(.''
L
( �.
Rep D
0
Concentration 7.4%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 / �-
Rep B 0
Rep C 0
Rep D 0
Concentration 14.8%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
Rep B 0
Rep C 0
Rep D 0

,F ENVIRONMENTAL LABORATORIES
,4 Division of LVerer Technology .and Conrrofs, Inc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility. Wilkesboro
NPDES #: NCO021717
Date of Test: 3/22/ 16
Initial weights taken on: s'- b
Final weights taken on: > 3 ` L
Page: 1 of 2
Outfall:
by; _ C �') Organism: Pimephales promelas
by: , i
PAN WT.
PAN + ORG.
WT. OF ORG.
MEAN
CONC
REP
(mg)
WT. (mg)
(mg)
# ORG.
WT./ORG. (mg)
SURV %
A
Uri
o
'
� 1 Y'
C
D
B
0.92%
--
C
Ll
A
B
1.85%
C
D

smv/RommemrAL LAaonATomss
F|SHLARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility- Wilkesboro
NPDES#� N
Date 0fTest. 3/77/16
\(
Initial weights taken on: ~" �� ' ) ` — � �0
Final weights taken on: T
Organism:
Page-2of2
(}u1faU:
CONC
REP
(Mg)
WT. (mg)
(Mg)
# ORG.
WTJORG. (mg)
SURV %
IT, T7

MERITECH, INC. Meritech Sample ID #:
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reed(a)meritech-labs.com Web Site: www.meritechlabs.com
CLIENT INFORMATION
Client:
k) V eS o a)
PO#:
Contact Person:
,tty1Y)ei 1"iJ
��/ `CS
NPDES#:
NC Q\CI.;t 1 j 1
Address:
'1 G e S n y C-1
f'r' �V ei2 t
Phone:
-7 _3 y
City:
v�i; I! PS h ` s ('
,
Pipe #:
G C
County:
`/u ��.t'
I
State: Zip:
INFORMATION
-
Sample Site:
r
lSAMPLE
Sample Type:
❑ Grab Composite # of containers:
Sampling Time: Start Date: `r�21 //�• Start Time: /Gr I PM
r
End Date: 2 End Time: rf� /�l 4� AM PM
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory—
Collector's Name: Print: h, cYt I7 EjYy ��r ` �`"S Signature:
TOXICITY TEST INFORMATION
Test Required: .Chronic (7 days) Test Organism: Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours) [ Pirnephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
iwc: �, 7 % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
Time: •,�/
AM
Relinquished by: Date: _j '�,�
Received by://7 I - i Date: Time: �,T / ��
AM
Relinquished byEJ:' •� , / � �_/ Date: -% ;�"z Time: 1 L•
AM
Received by: Date: Time:
AM PM
Relinquished by: Date: Time:
AM PM
Received by: Date: Time:
AM PM
Relinquished by: Date: Time:
AM PM
Received by: Date: Time:
AM PM
Sample Temperature (°C):
Method of Shipment: ❑ UPS ❑ Fed EX V4� Meritech Pick-up ❑ Delivered ❑ Other
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: -�
Received by: i `- Date: Time: i AM 2M
Sample Temperatures (°C): C"', J / I / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

r ,r,
MERITECH, INC. MeritechSample ID
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reed(o)meritech-labs.com Web Site: www,meritech-labs.com
y j CLIENT INFORMATION
Client: I C W l) �`j -� yti1 ;��, (`I� by 22 PO#.
Contact Person: K , vx0o e f \\j Y v , fieS NPDES#: NC Cl Ci `Z 1-1 ) 1
Address: �(✓� S Y1^► �' C Zet Phon : 6) (,C;-7 - 2,3 `"
City: Pipe #: L O I
County: State: jyL Zip: Cle 11
SAMPLE INFORMATION
Sample Site:
Sample Type: C -) Grab EXComposite
Sampling Time: Start Date:
End Date: --a (p
# of containers:
Start Time: 1740 V PM
End Time: l y I VAgM J PM
Triple rinse sample container with sample before filling. Completely Till the sample container with no air space_
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory—
Collector's Name: Print: KMXv Qei Signature:
Test Required: (Chronic (7 days)
❑ Acute (24-48 hours)
TOXICITY TEST INFORMATION
Test Organism: [ Ceriodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by:
Date: 3 7 p
Time: � ) �
AM
PM
Received by: _
-- Date: .3 l
Time: r
�. 2-P
AM
PM
Relinquished by:�
Date: _
Time: 3:_e
AM
PM
Received by:
Date:
Time:
AM
PM
Relinquished by:
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Relinquished by:
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Sample Temperature (°C):
Method of Shipment: ❑ UPS
❑ Fed EX K Meritech Pick-up ❑ Delivered
❑ Other
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLE RECEIVING (Laboratory Use Only
AVI
Relinquished by:,�% HOC
Received by: Date: ` Time: ^3I AM
Sample Temperatures (°C): l� a / 'c ! ! I Sample Condition: !
�� WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. MeritechSample ID
I
Bioassay Sample Chain Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: [e-mail] Web Site: _www.meritech-labs.com
FORMATION
CLIENT IN--
Clien
PO#:
Contact Person:
r
NPDES#: _NC & �) -2 I% f %
Address:
o S i t is t' i'
- tre t
q
Phone(
City:
`'Ic
1
Vyt I t�.P , ,OC,; ;
Pipe #:�y
County:
I V,;'S
State:
Zip:
SAMPLE INFORMATION
Sample Site:
Sample Type: U Grab Composite # of containers: i3
Sampling Time: Start Date: i31k? V l / &- Start Time: " t M j PM
End Date: ��; % [.� End Time: 0 `. 0 j `M PM
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory—
Collector's Name: Print: ;\irl �ly=�-t 1 �'�: `1�'C Signature: ?)lzf,=�,t?1 Zjr
TOXICITY TEST INFORMATION
Test Required: Chronic (7 days) Test Organism: Ceriodaphnia dubia (water flea)
Acute (24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: 7 ,'% % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by:
ell
Date:j
Time:
(, f
AM?
Received by:
/�
}—
j '., ` r
Date:
i / (
Time:
/ . �/�
AM
Relinquished by:
_�
—
!j /
Date:
Time:-,
:
AM
f?fvl
Received by:
Date:
Time:
AM
PM
Relinquished by:
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Relinquished by:
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Sample Temperature (°C):
Method of Shipment:❑ UPS ❑ Fed EX g Meritech Pick-up ❑ Delivered ❑ Other
" Samples shipped on Friday must be FedEx and must be clearly lab@led for Saturday delivery, NO SIGNATURE REQUIRED
SAMPLE RECEIVING (Laboratory Use Onl
1 i
Relinquished by:
Received by: r �, Date: I Time: i''` AM' PM
Sample Temperatures (°C): (; . r, / / / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

1
Title: wilkesboro
File: wilkes Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
----------------------------------------------------------------------------
D = 0.1010
W = 0.9428
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

1
Title: wilkesboro
File: wilkes Transform: NO TRANSFORMATION
'Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------
Calculated B1 statistic = 8.3098 (p-value = 0.1400)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
-------------------------------------------------------------------
Critical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)

4
Title: wilkesboro
File: wilkes Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------
SOURCE DF SS MS F
Between 5 0.0475 0.0095 1.6916
Within (Error) 18 0.1010 0.0056
Total 23 0.1485
(p-value = 0.1876)
Critical F = 4.2479 (alpha = 0.01, df = 5,18)
= 2.7729 (alpha = 0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha. = 0.05)

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:12/19/2016
Facility: Wilkesboro NPDES # NC00 21717 Pipe #: 001 County: Wilkes
(�La6ratory/. Meritech, Inc. Comments Single statistical inversion in the
•--------- 0.92% test concentration. The 0.92% test
Sighature of Operator in Resp Bible Charge concentration is not included in the final
x � / a v .�,��� 4 statistical results.
Signature of Laboratory Supervisor
Test Initiation Date/Time
% Eff. Repl.
Control Surviving It
Original it
Wt/original (mg)
0.92 Surviving It
Original 11
Wt/original (mg)
1.85 Surviving It
Original It
Wt/original (mg)
3.7 Surviving It
Original It
Wt/original (mg)
F-T.-T-1 Surviving It
Original It
Wt/onginal (mg)
14.8 Surviving It
Original It
Wt/original (mg)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp. at Receipt (°C)
MAIL ORIGINAL'I0: Water Sciences Section
Aquatic Toxicology Branch
Division of Water Resources
1621 Mail Service Center
Raleigh, N.C. 27699-1621
12/6/2016 1:40 PM
1 2 3 4
Avg Wt/Surv. Control 0.759
10
9
10
10
10
10
10
10
0.770
0.706
0.790
0.692
9
10
9
10
10
10
10
10
0.627
0.719
0.524
0.510
10
10
10
10
10
10
10
10
0.853
0.853
0.795
0.727
9
10
10
10
10
10
10
10
0.695
0.779
0.801
0.696
10
10
1 10
9
10
10
10
10
0.880
0.653
0.834
0.772
10
10
10
10
10
10
10
10
0.951
0.840
0.878
0.831
Day
% Survival 97.5
AvgWt(mg)F 0.740
% Survival 95.0
Avg Wt (mg) 0.595
% Survival 100.0
Avg Wt (m
g).F 0.807
'% Survival 97.5
Avg Wt (mg) 0.743
Oo Survival 97.5
Avg Wt (mg) 0.785
% Survival 100.0
Avg Wt (mg) 0.875
n 1 2 3 4 5 6
Test Organisms
7 Cultured In -House
f7 Outside Supplier
Hatch Date: 12/5/16
Hatch Time: 3:00 pm CT
8.02
/ 7.68
8.07
/ 7.61
8.14
/ 7.99
8.15
r 8.15
8.29
18.11
8.18
/ 7.72
8.19
! 7.64
7.73
/ 6.50
7.90
/ 6.20
7.75
! 7.57
7.95
r 7.78
8.07
r 7.71
7.45
/ 6.65
7.81
/ 6.20
25.4
/ 25.3
24.9
/ 24.6
24.8
! 24.6
24.7
/ 24.9
25.1
r 24.8
25.0
/ 25.5
25.1
! 24.8
n 1 3 4 5 6
7.95
r 7.71
8.01
/ 7.65
7.97
/ 7.99
8.08
1 8.15
8.20
/ 8.10
8.07
/ 7.76
8.06
! 7.76
7.68
/ 6.59
7.79
/ 6.00
7.63
/ 7.56
7.85
/ 7.97
8.05
/ 7.59
7.85
/ 6.57
7.79
/ 6.39
24.9
/ 24.8
25.9
/ 24.2
25.4
/ 24.9
24.6
/ 24.6
25.9
/ 24.8
25.0
/ 24.5
25.6
/ 25.2
1 2 3
12/5/2016
12/7/2016
12/8/2016
24.1
23.9
26.4
85
108
128
106
109
109
1036
1044
1232
<0.1
<0.1
<0.1
3.3
1.0
0.7
Dilution H2O Batch It 1098 1099 1100 1101 1102
Hardness (mg/L) 44 48 46 42 44
Alkalinity (mg/L) 51 59 59 50 58
Conductivity (umhos/cm) 193 221 199 197 221
Syrvival
Growth Overall Result
-81
Normal
IFS
ir-11 ChV >1-4
Hom. Var.
IF 1
F171
NOEC
14.8
14.8
LOEC
>14.8
>14.8
ChV
>14.8
>14.8
Method
Steel's
Dunnett's
Stats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
0.92
10 N/A
10 20
2.41 N/A
2.41-1.4527
1.85
3.7
10 18
10 18
2.41-0.0699
2.41-0.9738
7.4
14.8
1 10 20
2.41-2.9161

Client:
Wilkesboro
NPDES #: 0021717
Initiated by:�� L'
Date/Time Fed: 12/6/16
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber: 10
Test Vessel Size: 400 ml
Test Solution Volume: 250 ml
Temp. of Stock: X7 °C
Date/Time Born: 12/5/16 3:00 PM CT # of Reps: 4
Organism Source: Aquatox, Inc. Analyst(s): MR, CD, LV, SB
Transferred by:
Date
Time Initials
Day 1
12/7
01 G✓�
Day 2
12/8
� '1
Day
12/9
�l (1�jG�( �>✓� ��'�
Day 4
12 10
Day 5
12/11
Day 12/12 21/ )_3
Test Termination Data:
Initials/Signature: L✓�=' �„ Sri f�
End Date: 12/13/16
Start Date: 12/6/16 Time:
End Date: 12/ 13/ 16 Time: / / C (,�� i✓�
Randomization: Yes No
Incubator #:
Fed by:
Date
Time 1
Initials
Time 2
Initials
Day 0
12/6
; t / U/?✓�
V� rr
1�
Day 1
12/7
Day 2
12/8''
f)i�';� ,`
/ ✓
l=':,N �/� �1��
Day
12/9
=``;i�iZrt%I
L✓7G��
Day 4
12/ 10
Day 5 12/11 �G` :_w. Cg?
Day 12/12 G. j�'i,,r /vt

ENVIRONMENTAL LABORATORIES
4 Division of Water Technology and Controls, lnc
Chemical and Physical Determinations
Client: Wilkesboro NPDES#: 0021717 Start Date: 12/6/16
Test Organism: Plmephales promelas End Date: 12/13/16
Da
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Freshwater
Batch # :
y�
V C�
�U9
Cr�O�
�� ``
V
l �
pJ+ Initial
`- ,�
��•r;7
i 5
cox
i 1
Final
7
Co (
1M61
1 • (5
V.� (
�
U.O. Initial
1
7• 7
- d 7
5�
Final
(� � S G
I' �
7 � �
% t
l (12S
lY•
Temp.: Initial
Y, cl
q • X
S'
Final
5
u
•
, f
`1. E�
`1-S.
'
�r
Conductivity: Initial
q, c f316,
`-17 �;
Final�-
Residual Chlorine:
L O, (
L.O• (
d (
< i
L� (
��•
<�•
Hardness:
Alkalinity:
'151
Dav
Concentration: 0.92%
0-1
1-2
2-3
3-4
4-5
6-7
Remarks
pH: Initial
, �J
, / oZ
U
��
`�«
G5-6
o
c
bLi
Final
% to ��
D.O.: Initial
�vp4
7
�-
�-
�• �/�j
7.�
Final
C, - Li
S,
Ll
Temp.: Initial
Final
9Q, t,1
0�
Conductivity: Initial
Final
T 3
2
abT
aS
Residual Chlorine:
I Z-0.1
L . (
O. l
G 0 • i
Dav
Concentration: 1.85%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
r3
R, �i>
Final
7, 6 a
D.O.. Initial
Final
Temp.: Initial
Final
�7 ,
ti.
5 - �-
�� `�
aL4
if ,
Conductivity: Initial
Final
►��-
S
�
Residual Chlorine:
L0, 1
L-(� . r
L O)
40.1
G ,

MER/TECH, ff~C
is - X
EN VIRON Mc NTAL LABO RATO RIESr,c
.4 Oiv(slo� c./ Ut ar r Tocl.r•o/opy and Co••fresls, I
Chemical and Physical Determinations
Page 2 of 2
Client: Wilkesboro NPDES#: 0021717 Start Date: 12/6/16
Test Organism: Pimephales promelas End Date: 12/13/16
Day
Concentration: 3.7%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial:
VI
A
r l,7
,J3
1Z—
Final
77
5`1
q `1 07
r'
GI
v S
llb- '
D.O.: Initial
{
11
`�, c (p
"7-
Final
L- . 7
­5( a
S -
-76
Temp.. Initial
` ,3, (.O
";S
�-j- i
Final
7
, H,
Conductivity: Initial
r ��
�
a (��
2-do
J1
Final��-
Residual Chlorine:
Zv, l
L O.
G O.
G U.
Z-G. j
G D
Dav
Concentration: 7.4%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
, U
,01)
`S
,
' ��
r l%
�,fil✓
Final
7- G'
'1- Q- "
D.O.: Initial
7 X3
41
�
7.
Final
G--Y
U.
7 -�-��
�`l
Temp.: Initial
9t .
5 >S
`1, 3
, q
Final
Lj.
�t •
-U U14
Conductivity: Initial
r,),5C)
r SA-)
�`�'�
v �'Q
Final
`6
rW.
' ,
- Gj
1
t
Residual Chlorine: I
e_a I
Z-. f
Dav
Concentration: 14.8%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
Final
7,71
(DG?
"l
p;
D.O.: Initial
7. 77
Final
(Q S j
, c,L
j
Temp.. Initial
I(.
j,�,
Final
7L(.
IU-d
Conductivity: Initial
7 610
`j X-1
7 kS"
Final
Residual Chlorine:
ZO. i
zC�. I
Z O. l
LC; 1
GO I
Z_G.
Day
Concentration: 100%
0-1
1 1-2
2-3
3-4
4-5
1 5-6
6-7
Remarks
pH:
7- 4- O
_?-
Conductivity:
162
0Lfq/
3�z
Residual Chlorine:
L p,
LO.
.40.1
Hardness:
V'
Alkalinity:
10

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Wilkesboro NPDES#: 0021717
Test Organism: Pimephales promelas
Concentration Control
Start Date: 12/6/16
End Date: 12/13/16
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep 0
Rep B 0
Rep C 0
Rep D 0
Concentration 0.92%
Tuesday Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A 0
0
Rep B 0
Rep C 0 v
�J
y
(,/
v
,' �-✓ i�-i3 lL
D
Rep 0
v
Concentration 1.85%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
Rep B 0
�
�r
Rep C 0
Rep D 0
�J I

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client:
Wilkesboro NPDES#: 0021717
Start Date:
12/6/16
Test Organism:
Pimephales promelas
End Date:
12/13/16
Concentration 3.7%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday
Rep A 0
Rep B 0
Rep C 0
Rep D 0
Concentration 7.4%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday
Rep A 0
U
Rep B 0 V �� `✓ ��
Rep C 0
Rep D 0
Concentration 14.8%
Tuesday Wednesday Thursday Friday Sa-turday Sunday Monday
Rep A 0
Rep B 0 U
z�
`i
��
Rep C 0
Rep D 0
Tuesday
Tuesday
Tuesday

A07HR/TECH, /NC_
ENVIRONMENTAL LABORATORIES
!.. A Division of L Vafer Technolo Jy and controls, inc.
FISH LARVAL SURVIVALIGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Wilkesboro
NPDES #: 0021717
Date of Test: 12/6/16
Initial weights taken on- by: ! V
Final weights taken on: ', _ j�_ .� by: L11
Page: 1 of 2
Outfall-
Organism: Pimephales promelas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WT.IORG. (mg)
SURV %
A
23 O
�y� . ��>
7 -7U
i D
O. 7 7 v
100
B
y
D C�
7�D�
C�
vrr766
qLI
0
c
237oQ,
2q y^ qa
-7. i O
0. -N a
00
B
2a6-73
9 33h q'
i sf
10
6, -� 1
16 D
0.92%
A
���«
' 5
I �
C�.3
Gov
B
S-Y.7
1.85%

ENVIRONMENTAL LABORATORIES
A Division of Water Techno/agy end Controls, Inc
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Wilkesboro Page: 2 of 2
NPDES #: 0021717 Outfall:
Date of Test: 12/6/16
Initial weights taken on: J j - .:-j by:
Final weights taken on. L-- by: L--1�'
Organism: Pimephales promelas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV
o
3.7 /o
A
6.
7,
B
2Ljy. ` 5
p��
�. i �(
v, 7 7
lG
A
? I
S'So..q I
7.4�ia
B
p 3�? g5
5. 3 E
6_ /�
235 61
RLI 3. g;
P q
0. K3
D
7- �j
�'
c- 7,�
��>
A
14.8%
B
30,30
0'��qo
O
C
) 6
7' q. G C
) .7
;

MERITECH, INC. Meritech Sample ID #:
Bioassay Sample Chain of Custody
- 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reed(a)meritech-labs.com Web Site: www.meritech-labs.com
Town of Wilkesboro VT INFORMATION NPbE8#. NC 0021717
C 700 Snyder Street PH [phone]
Contact Pei Wilkesboro, NC 28697 FAX [phone]
Add Attention: Kimberly Wiles mail: . ` [e-mail]
001
County: 0 �� S State: Zip:
�+ �+ SAMPLE INFORMATION
Sample Site: ri, incd t5 i' i' U ery�
Sample Type: ❑ Grab Composite # of containers:
Sampling Time: StartDate: Start Time: . V5� AM PM
End Date: 1 2_6 -/6 End Time: PM
*** Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory—
Collector's Name: Print: b POVSignature:
(t/%X
9
- - —
s
TOXICITY TEST INFORMATION
Test Required: Zchronic (7 days)
Test Organism: Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours)
KPimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: %
Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMAT/I-ONjj
L,0
Relinquished by: /.�-��
Date: �.�-Ct1 _/k Time: S
AM
PM
Received by:
— Date: �- 6-- l ([i Time: -�
PM
Relinquished by: /w 2 �t _.���
Date: 6 9'-K •—%C7 Time:
AM
PM
Received by:
Date: Time:
AM
PM
Relinquished by:
Date: Time:
AM
PM
Received by:
Date: Time:
AM
PM
Relinquished by:
Date: Time:
AM
PM
Received by:
Date: Time:
AM
PM
Sample Temperature (°C):
Method of Shipment:[] UPS
❑ Fed EX K Meritech Pick-up ❑Delivered ❑ Other
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
1 \ SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
Received by: Date: C L f Time: 1,1 (/ AM PM
Sample Temperatures (°C): Sample Condition: c(�
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. Meritech Sample ID #:
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reed(a)meritech-labs.com Web Site: vr-- ` ` '-"-
- -- ---
NPDES#
NC 0021717
Town of Wilkesboro FORMATION
PH
[phone]
Client:
700 Snyder Street
FAX
Email:
[phone]
[e-mail]
Contact Person:
Wilkesboro, NC 28697
Address:
Attention: Kimberly Wiles -
City:
_
Pipe #: (_
OI
County:
Res State:
Zip:
SAMPLE INFORMATION
�+
I(Aen 1
Sample Site:
n0-1 T
Sample Type:
❑ Grab ,Composite # of
containers:
� -16 ,
Sampling Time:
Iis-7
Start Date: � Start Time: 7 q
.h
AM PM
End Date: 1, -- —ap 4— End Time: `7
AM PM
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory''
Collector's Name: Print: K;mber I.1 tIV 1 �es Signature:
Test Required: Chronic (7 days)
❑ Acute (24-48 hours)
IWC:3.'7 %
TOXICITY TEST INFORMATION
Test Organism: XCeriodaphnia dubia (water flea)
K Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions):
Comments-
SHIPPING INFORMATION
Relinquished by: Date: l- -l/�o Time: PM
R lve AM d b S Date: f� ! Time: I'D �S-o PM
ecey. ,
Date: %>- ��
Time: , �-
AM
PM
Relinquished by:
4011`
Received by:
Date:
Time:
AM
PM
Relinquished by:
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Relinquished by:
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Sample Temperature (°C):
Method of Shipment: ❑ UPS ❑ Fed EX 'N Meritech Pick-up ❑ Delivered ❑ Other
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: � `
Date: Time:�M
Received b�APM
Sample Temperatures (°C): / � :y / /
Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. MeritechSample lD#: 1:vl (� IL'
Bioassay Sample Chain of Custody
" 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reedCa meritech-labs.com Web Site: wwv
NPDES# NC 0021717
ORMATION PH [phone]
Client: Town of Wilkesboro FAX [phone]
700 Snyder Street
Contact Person:— Wilkesboro, NC 28697 Email: [e-mail]
Address:— Attention: Kimberly Wiles
City: _ Pipe #: 0
County: W 1 ke S State: Zip:
�+ SAMPLE INFORMATION
Sample Site: 114 ent
Sample Type: ❑ Grab -Composite # of contaiinne�
-rrs: _
Sampling Time: StartDate: IQ ^ 9- f � rI Start Time: / . Y 3 C-� PM
End Date: l a A —/ �o End Time: P : 0 7 �� PM
*" Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0'C upon receipt at the laboratory'
Collector's Name: Print: k-mbeylIV WhIP T Signature: r,(%
TOXICITY TEST INFORMATION
Test Required: ® Chronic (7 days) Test Organism: [� Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours) Pimephales promelas (fathead minnow)
2 r] ❑ Mysidopsis bahia (shrimp)
3 mc: • / % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by: Date:
Time:
AM
PM
Received by:
Date:
Time:
�
5 �j
13
PM
Relinquished by:
Date: j �l
Time:
/ : 1
AM
j_2
Received by:
Date:
Time:
AM
PM
Relinquished by:
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Relinquished by:
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Sample Temperature (°C):
Method of Shipment: ❑ UPS ❑ Fed EX X[ Meritech Pick-up ❑ Delivered
❑ Other
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
o
Received by/
Date: _ �,
Time:
j 1 i 1 )
AM
PM
i
Sample Temperatures (°C): /
/ �. / �� i / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

Title: Wilkesboro
File: Wilkes Transform: NO TRANSFORMATION
Shapiro - Wilkes Test for Normality
----------------------------------------------------------------------------
D = 0.0648
W = 0.9539
Critical W = 0.8680 (alpha = 0.01 , N = 20)
W = 0.9050 (alpha = 0.05 , N = 20)
Data PASS normality test (alpha = 0.01). Continue analysis.

'Title: Wilkesboro
File: Wilkes Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 1.9296 (p-value = 0.7487)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
----------------------------------------------------------------------------
Critical B = 13.2767 (alpha = 0.01, df = 4)
= 9.4877 (alpha = 0.05, df = 4)

Title: Wilkesboro
File: Wilkes Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE DF SS MS F
----------------------------------------------------------------------------
Between 4 0.0493 0.0123 2.8540
Within (Error) 15 0.0648 0.0043
----------------------------------------------------------------------------
Total 19 0.1141
----------------------------------------------------------------------------
(p-value = 0.0608)
Critical F = 4.8932 (alpha = 0.01, df = 4,15)
= 3.0556 (alpha = 0.05, df = 4,15)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title:
Wilkesboro
File:
Wilkes
Transform: NO
TRANSFORMATION
Dunnett's Test -
TABLE 1 OF 2
Ho:Control<Treatment
----------------------------------------------------------------------------
TRANSFORMED
MEAN CALCULATED
IN SIG
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
------------------
T STAT 0.05
------ ---
-------------------------
1
Control
-----------
0.7395
0.7395
2
1.85
0.8070
0.8070
-1.4527
3
3.7
0.7428
0.7428
-0.0699
4
7.4
0.7848
0.7848
-0.9738
5
14.8
0.8750
0.8750
-2.9161
----------------------------------------------------------------------------
Dunnett
critical value = 2.3600 (1 Tailed,
alpha = 0.05, df
= 4,15)
Title: Wilkesboro
File: Wilkes Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 2
OF 2 Ho:Control<Treatment
----------------------------------------------------------------------------
NUM OF
MIN SIG DIFF
o OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS
-------
(IN ORIG. UNITS)
----------------
CONTROL
-------
FROM CONTROL
------------
-----
1
--------------------
Control
4
2
1.85
4
0.1097
14.8
-0.0675
3
3.7
4
0.1097
14.8
-0.0033
4
7.4
4
0.1097
14.8
-0.0453
5
----------------------------------------------------------------------------
14.8
4
0.1097
14.8
-0.1355

Title: Wilkesboro
File: Wilkes Transform: NO TRANSFORMATION
Shapiro - Wilkes Test for Normality
----------------------------------------------------------------------------
D = 0.0225
W = 0.6749
Critical W = 0.8680 (alpha = 0.01 , N = 20)
W = 0.9050 (alpha = 0.05 , N = 20)
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning - The first three homogeneity tests are sensitive to non -normality
and should not be performed with this data as is.

Title: Wilkesboro
File: Wilkes Transform: NO TRANSFORMATION
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
------------------------------------------------------------
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.
-----------------------------------------------------------------

Title: Wilkesboro
File: Wilkes Transform: NO TRANSFORMATION
Steel's Many -One
Rank Test
- Ho:
Control<Treatment
----------------------------------------------------------------------------
MEAN IN
RANK
CRIT.
SIG
GROUP
IDENTIFICATION
ORIGINAL UNITS
SUM
VALUE
DF 0.05
---
-------------------------
1
Control
-----------
0.9750
-------
------
-----
2
1.85
1.0000
20.00
10.00
4.00
3
3.7
0.9750
18.00
10.00
4.00
4
7.4
0.9750
18.00
10.00
4.00
5
14.8
1.0000
20.00
10.00
4.00
----------------------------------------------------------------------------
Critical
values are 1 tailed ( k = 4 )

.J ep let -A h.y u I 'c'
Efflue.it Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:9/28/2016
Facility: Wilkesboro NPDES # NC00 21717 Pipe #: 001 County: Wilkes
. Naboatory: Mgkitech, Inc.
x
Signature Q Operator in Respon$ible Char
x �/� -�
Signature of Laboratory Supervisor _
Comments
Single Statistical inversion in the 0.92% test
concentration. The 0.92% test concentration
is not included in the final results.
MAIL ORIGINAL TO: Water Sciences Section `u f Y
Aquatic Toxicology Branch
Division of Water Resources
1621 Mail Service Center
Raleigh, N.C. 27699-1621
Test Initiation Date/Time 9/13/2016 1:50 PM Avg Wt/Sun/. Controll 0.722
% Eff. Repl. 1 2 3 4
Control I Surviving #
Original #
Wt/original (mg)
0.92 1 Surviving #
Original #
Wt/original (mg)
1.85 1 Surviving #
Original #
Wt/original (mg)
10
9
10
10
10
10
10
10
0.648
0.733
0.649
0.776
9
10
9
1 10
10
10
10
10
0.449
0.556
0.530
0.638
10
10
10
10
10
10
10
10
0.639
0.660
0.735
0.825
% Survival 97.5
Avg Wt (mg) 0.702
Test Organisms
C' Cultured In -House
i✓ Outside Supplier
Hatch Date: 9/12/16
Survival 95.0 Hatch Time: 3:00 pm CT
Avg Wt (mg) F 0.543
% Survival 100.0
Avg Wt (mg) 0.715
3.7 1 Surviving #
10
10
10
10
% Survival 100.0
Original #
10
10
10
10
10
10
10
10
0.738
0.664
0.608
0.715
14.8 Surviving #
% Survival
100.0
Original #
WUoriginal (mg)
Avg Wt (mg)
0.732
Water Quality Data
Day
Control
0
1
2
3
4
5
6
pH (SU) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp. at Receipt (°C)
7.95
/ 7.67
8.01
/ 7.85
8.18
! 7.68
8.11
/ 8.05
8.17
7.88
8.34
/ 7.97
8.19
/ 7.92
n 1 9 R 4 5 6
7.88
/ 7.66
7.94
/ 7.64
7.96
/ 7.85
8.00
/ 8.18
8.26
/ 7.87
8.17
/ 8.03
8.13
/ 7.95
7.84
/ 6.90
7.85
/ 6.56
7.83
/ 7.03
7.57
/ 7.81
8.10
/ 6.34
8.10
/ 7.52
7.72
/ 7.69
24.6
/ 24.6
24.9
/ 24.5
25.1
/ 25.4
24.2
/ 25.4
24.8
/ 24.7
24.9
/ 24.5
24.6
/ 25.8
1 2 3
9/12/2016
9/14/2016
9/15/2016
21.2
24.1
25.9
98
138
146
108
119
153
1102
1221
1200
<0.1
<0.1
<0.1
1.4
1.3
1.1
Dilution H2O Batch # 1068 1069 1070 1071
Hardness (mg/L) 44 48 44 44
Alkalinity (mg/L) 51 53 54 53
Conductivity (umhos/cm) 194 211 203 208
Survival
Growth
Normal
irl
F1
Hom. Var.
(r'i
FI
NOEC
14.8
14.8
LOEC
>14.8
>14.8
ChV
>14.8
>14.8
Method
Steel's
Dunnett's
Overall Result
ChV
Stats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
0.92
10 16
2.41 Not Included
1.85
10 20
2.41-0.3025
3.7
10 20
2.41-0.7818
7.4
10 20
10 20
2.41 0.4622
2.41-0.6848
14.8
10
10
10

Client: Wilkesboro
NPDES #: 0021717
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber: 10
Test Vessel Size: 400 ml
Initiated by: G-.:S: Test Solution Volume: 250 ml
Date/Time Fed: 9/13/16 GI��� Temp. of Stock: all -°C
Date/Time Born: 9/12/16 3:00 Pm CT # of Reps: 4
Organism Source: Aquatox, Inc.
Transferred by:
Date
Time
Initials
Day 1
9 / 14
Day 2
9/ 15
Day 3
9/ 16
Day 4
9/ 17
Day 5
9/18
Day 6
9/ 19
`� '-at'�•-
/n��r>
Analyst(s): MR, CD, LW, S
Test Termination Data: /
Initials/Signature: ('j)
End Date: 9/20/16
End Time: j � V' nR�
Start Date: 9/ 13/ 16 Time: 3YA
End Date: 9/20/16 Time:
Randomization: Yes No
Incubator #:
Fed by:
Date
Time 1 Initials
Time 2 Initials
Day
9/13
Day
9/14
f rL
Day 2
9 / 15
Day 3
9/16
1 S
' -- U w- Cam,
Day 4
9 / 17`
�� L,�/hILL
Day 5
9 / 18
Day
9/19
Li

7—�EC7v, ®AjfC7.
ENVIRONMENTAL LABORATORIES
A Division cf Wafer Technology and Controls, Inc
Chemical and Physical Determinations
Client: Wilkesboro NPDES#: 0021717 Start Date: 9/13/16
Test Organism: Pimephales promelas End Date: 9/20/16
r DAV
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Freshwate
Batch # :
'Ll 79 _
pH: Initial
U
^' (��
Final
�;
$ .
%
D.O.: Initial
-7,`, 1
7.
7
Final
. �'�
..�jU
��
7 `//
6-CbA
LA'5
7, 74
Temp.: Initial
Final
7,S. L
:� `i - J
Conductivity: Initial
Zl 1
l D
5-
Final
VW�
Residual Chlorine:
Ld. (
Gb. (
L �.
L`' 1
Hardness:
q,1.
Ll z
Alkalinity:
1
3
�;-'
-'
Dav
Concentration: 0.92%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
Final
%:
qq 4
D.O.: Initial
�•���
�'
Z
Final
7"Temp.:
Initial
�La
,�
25-S
KZ,579,,
Final
"' S ,�1
2 ,�
,� (_a
%
1 _Conductivity:
Initial
d
2.2�
90
-� ?
'L
f
�- l
Final`3
,
t3
Residual Chlorine:
?Q, (
o
G
L.
C 0 ` 1
L_()' •
Lc
Dav
Concentration: 1.85%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
Final
7,
�.
, �i U
''S
% r ,'
-7,
, S' b'
D.O.: Initial
1.-7.
Final
CIS
7-f3
L,I-i.3
7. 15
Temp.: Initial'
Final
,1f
Lj
{ .�.
�; J `i
' -71
Conductivity: Initial
•�
3J,
Final
a L13
iri
Residual Chlorine:

`WA=- "® 7TA=74C:-H, ®~C:7-
ENVIRONMENTAL LABORATOFtIES^c
A O/vlslo-s c.f N..t¢� 15pch�o/ogy wed Co.r.-ols. /
Chemical and Physical Determinations
Page 2 of 2
Client: Wilkesboro NPDES#: 0021717 Start Date: 9/13/16
Test Organism: Pimephales promelas End Date: 9/20/16
l)av
Concentration: 3.7%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
7. 3
.
r
�Sr ,
V
�' S
Final
.7l
-7-.
I i"
eY. /)�
,l
� •�il'�
�
D.O. Initial
-7. Q'7
� 1
`7. _
Final
�
7 �`/
Temp.: Initial
1 `z
Final
LU / 5aiZ
Conductivity: Initial
Final
2,S2
d6 U
Residual Chlorine:
4-- D.
G(}. I
< <J
L_ 0
nav
Concentration: 7.4%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
-7 . `r a
-7
Final
-%. 7'
7 6
1,114
Y - (6
`7."
'P6-LI
`j 77
D.O.. Initial
. `�
�- �,-'�
. 5ET
k G 7
Q r (1
Final
73
Temp.: Initial
U,
3`t
Final
V
y .
Conductivity: Initial
?
Final
Z
�(
!�
Residual Chlorine:
Zo, t
40. L`'
e> • I
< <�
t' :
�� 1
Dav
Concentration: 14.8%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
-7, u
-7,q
7
Final
(jj-
%
'71
" I
,:3
7. 5
D.O.: Initial
, Ti7
-7,79
? -E�7)
I
Final
Temp.: Initial
Final
e;,Z�
Conductivity: Initial
3
��.,
1 %
��-�
-3 `t
Final
93
S
1J
j
Residual Chlorine:
Zb •
c' , I
E`-v
.
�-
1
111 V
Concentration: 100%
0-1
1-2
2-3
3-4
1-5
5-6
6-7
Remarks
pH:
% L
%- 7
Conductivity:
1 a
Residual Chlorine:
no. I
Hardness:
f
Alkalinity:
`L'
1
�'

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Wilkesboro NPDES#: 0021717
Test Organism: Pimephales promelas
Concentration Control
Start Date: 9/13/16
End Date: 9/20/16
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
yRep A 0
Rep B 0
Rep C 0
Rep D 0
Concentration
0.92%
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
Rep B
0
Rep C
0
Rep D
0
Concentration
1.85%
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
Rep B
0
Rep C
0
r
Rep D
0

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client:
Wilkesboro
NPDES#:
0021717
Start Date:
9/13/16
Test Organism:
Pimephales
promelas
End Date:
9/20/16
Concentration
3.7%
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
Rep B
0
Rep C
0
v
��
Rep D
0
Concentration 7.4%
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A 0
Rep B 0
Rep C 0
Rep D 0
Concentration
14.8%
Tuesday
Wednesday Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
r)
Rep B
0
Rep C
0
`-
v
Rep D
0
V
G�
I
1✓
V

ENVIRONMENTAL LABORATORIES
A Division Of Wfer Technology and Controls. Inc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility-. Wilkesboro
NPIDES #-. 0021717
Date ofTest: 9/13/16
Initial weights taken on: by: Z—L./
Final weights taken on: l by: CQ,
Page: 1 of 2
Outfall:
Organism: Pimephales promelas
CONG
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WT./ORG. (mg)
SURV %
A
4�
B
_T 1
—7
0
C
25,q,
-79
C/
D
!NG,3L
1/7
A
B
0.92%
C
(Oc
D
A
B
1.85%
73
D
-32

��
~~~~~�~~ff ~^�^��~~*r ff~~~`~�~
smvmomxxemnAL LAaonAromss
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
F8Ci|hv� Wilkesboro
NPDES#- (}021717
D8te0fTest: 9/13/16
Initial weights taken on: by:Final weights taken
ii
by-
Page- 3 of
{)UtfaU�
Organism- Pimephales promelas
CONC I
REP
(mg)
WT. (mg)
(mg)
# ORG.
WTJORG. (mg)
SURV %
jpc
71
A
lag
Sol/
7i3G

MERITECH, INC. Meritech Sample ID#: yc�131"'',�
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reed(cDmeritech-labs.com Web Site: www.meritech-labs.com
Client: .
Contact Person: on
Address: 00 ,-)
City: ; 1 VPS
County: 1 Ve 3
CLIENT INFORMATION
State: VrC
PO#: _
NPDES#: NC O C Z! 7 /2
Phone:(334)
Pipe #:' w!
Zip: �t �jPi69 ?
j� r (� SAMPLE INFORMATION
Sample Site: l l�na-d
Sample Type: ❑ Grab Composite # of containers: T
Sampling Time: StartDate: CI -I .2 % 62 Start Time: / b q(� AA PM
End Date: l '1.3 ! 6 End Time: 'j 0 & PM
Triple rinse sample container with sample before filling. Completely fill the sample container with no airspace.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory—Collector's Name: Print: klrY7 heyli I icy Signature: �lyyvf L--'1,4 I JACA
TOXICITY TEST INFORMATION v
Test Required: .Chronic (7 days) Test Organism: Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours) ❑ Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Comments:
Relinquished
Received
Relinquished
Received
Relinquished by:
Received by:
Relinquished by:
Received by:
IWC: 3-7 % Test Concentrations (if multiple dilutions):
SHIPPING INFORMATION
Date:
—
Date:
r •• j 3 j
Date:
Date:
Date:
Date:
Date:
Date:
Sample Temperature (°C):
Time: S-6— CAW'IPM
Time:
f17; S u`' 4 PM
7• ��
Time:
AM
I-P2A/
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Method of Shipment: ❑ UPS ❑ Fed EX X Meritech Pick-up ❑ Delivered ❑ Other
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
/ SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: �vC-•-� - %�^-�
Received �� Date: 1 3 ` Time: I �/ AM QP
,
Sample Temperatures ('C): Sample Condition: G %
WHITE = Laboratory copy YELLOW = Client copy ��

MERITECH,INC. MeritechSample ID
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reed(a)meritech-labs.com Web Site: _www.meritech-labs.com
CLIENT INFORMATION
Client:
u) () U-C
} 1
1 , 1 NIPS b6frC
PO#:
Contact Person:
NPDES#: NC U -V / /
Address:
�7
oosny d i/
6+r�
Phone: 3,3& -2 3
City.
'Apt
V �+ 1 I K I, e S
Pipe #: 601
1
County:
W i-S
n'
State: /yc - Zip:
SAMPLE INFORMATION
c
r ' nQ�
`
ETT / !.(e n +
Sample Site:
Sample Type:
❑ Grab MComposite # of containers:
_
Sampling Time: Start Date: /Q' 1,4-1 V Start Time: •'75 2 ® PM
End Date: % - i S % L End Time: / S g M PM
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory`**
Collector's Name: Print: n I �(�j l�% �i IC'S Signature: 0",6�q
Test Required: %6hronic(7days)
❑ Acute (24-48 hours)
IWC: ;3. 1] %
Comments:
TOXICITY TEST INFORMATION
Test Organism: f�z Ceriodaphnia dubia (water flea)
[gPimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions):
�n
Relinquished by: G
Received by: �—
Relinquished by:�-
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
PPING INFORMATION
Date:
Date:
Date: �5�16
Date:
Date:
Date:
Date:
Date:
Sample Temperature (°C)
Time: /0 L�S �� PM
Time: IO L/ S <Em) PM
Time: AM iPM /
Time: AM PM
Time: AM PM
Time: AM PM
Time: AM PM
Time: AM PM
Method of Shipment: ❑ UPS ❑ Fed EX �4 Meritech Pick-up Delivered ❑ Other
Samples shipped on Friday must be Fed Ex and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
Received by:: ,�„ Date: 7,// ( Time: AM
Sample Temperatures (°C): L : 7 / 1 ) / / Sample Condition: C n
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. Meritech Sample ID M 0_.'
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
�) CLIENT INFORMATION
Client: L)0 O � v v t' �G Z% PO#:
Contact Person: *7: w1 r r t `F S NPDES#: NCC)C) -Z / ,7 17
Address: it der " e0jL PhoneC33C.-) (,-, (, 7-,2 39
City: lv i) Vf Sb('ro Pipe#: 06 /
County: S. State:—�= Zip:`(✓ /
SAMPLE INFORMATION
Sample Site: 1 i 6 CO��ty E � - l y en '
Sample Type: ❑ Grab D� Composite # of containers:
Sampling Time: Start Date: (�'l7 [ t✓ Start Time: / 5U AM PM
-1 End Date: 46 " 6 End Time: ql�b (M PM
*** Triple rinse sample container with sample before filling. Completely fill the sample container with no airspace.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory***
Collector's Name: Print: "'J /y VV i I�S Signature:
TOXICITY TEST INFORMATION
Test Required: $ Chronic (7 days) Test Organism: [;,Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis Bahia (shrimp)
1WC: ; j, ' % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by: � Date: —-; Time: ( PM
PM
Received by: 2 Date: g %�j — ('�/ Time:
Relinquished by: ✓ (_WL
Date: 7 "'�t�j<-- lly Time: r o
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature (°C):
Method of Shipment: ❑ UPS ❑ Fed EX K Meritech Pick-up ❑ Delivered ❑ Other
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED *'
SAMPLE RECEIVING (Laboratory Use Only)
t 1 ,r
Relinquished by:_)'-,
-ram
Received by: / - `I Date: t / I ?- / `: I. Time: S c (�l AM CPM
Sample Temperatures (°C): 1 , \ / 1 . Sample Condition:
�� WHITE = Laboratory copy YELLOW = Client copy

Title: Wilkesboro
File: WilkesGrowth Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
----------------------------------------------------------------------------
D = 0.0576
W = 0.9452
Critical W = 0.8680 (alpha = 0.01 , N = 20)
W = 0.9050 (alpha = 0.05 , N = 20)
Data PASS normality test (alpha = 0.01). Continue analysis.

'Title: Wilkesboro
File: WilkesGrowth Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
-----------------------------------------------------------------
Calculated B1 statistic = 2.8324 (p-value = 0.5863)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
-------------------------------------------------------------------
Critical B = 13.2767 (alpha = 0.01, df = 4)
= 9.4877 (alpha = 0.05, df = 4)

Title: Wilkesboro
File: WilkesGrowth Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE DF SS MS F
----------------------------------------------------------------------------
Between 4 0.0080 0.0020 0.5219
Within (Error) 15 0.0576 0.0038
----------------------------------------------------------------------------
Total 19 0.0656
----------------------------------------------------------------------------
(p-value = 0.7212)
Critical F = 4.8932 (alpha = 0.01, df = 4,15)
= 3.0556 (alpha = 0.05, df = 4,15)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title:
File:
Wilkesboro
WilkesGrowth
Transform: NO
TRANSFORMATION
Dunnett's Test -
TABLE 1 OF 2
Ho:Control<Treatment
____
---------------
TRANSFORMED
MEAN CALCULATED
IN SIG
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
-----------
T STAT 0.05
-------------------------
1
Control
-----------
0.7015
0.7015
2
1.85%
0.7148
0.7148
-0.3025
3
3.7%
0.7358
0.7358
-0.7818
4
7.4%
0.6813
0.6813
0.4622
5
14.8%
0.7315
0.7315
-0.6848
-----------------------------------------------------------
Dunnett
critical value = 2.3600 (1 Tailed,
alpha = 0.05, df
= 4,15)
Title: Wilkesboro
File: WilkesGrowth Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 2
OF 2 Ho:Control<Treatment
----------------------------------------------------------------
NUM OF
MIN SIG DIFF
% OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS
-------
(IN ORIG. UNITS)
----------------
CONTROL
-------
FROM CONTROL
----
-----
1
--------------------
Control
4
2
1.85%
4
0.1034
14.7
-0.0132
3
3.70-o
4
0.1034
14.7
-0.0342
4
7.4%
4
0.1034
14.7
0.0203
5
--------------------------------------------------------------------------
14.8%
4
0.1034
14.7
-0.0300

Title: WilkesSury
File: WilkesSury Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
----------------------------------------------------------------------------
D = 0.0175
W = 0.7713
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning - The first three homogeneity tests are sensitive to non -normality
and should not be performed with this data as is.

-Title:
WilkesSury
File:
WilkesSury
Transform:
NO
TRANSFORMATION
Steel's Many -One
Rank Test
- Ho:
Control<Treatment
----------------------------------------------------------------------------
MEAN IN
RANK
GRIT.
SIG
GROUP
IDENTIFICATION
ORIGINAL UNITS
SUM
-------
VALUE
------
DF 0.05
----- ---
-------------------------
1
Control
-----------
0.9750
2
0.920-.
0.9500
16.00
10.00
4.00
3
1.85%
1.0000
20.00
10.00
4.00
4
3.70-o
1.0000
20.00
10.00
4.00
5
7.40-o
1.0000
20.00
10.00
4.00
6
14.8%
1.0000
20.00
10.00
4.00
----------------------------------------------------------------------------
Critical
values are 1 tailed ( k = 5 )

SAMPLE
DATES
6/10 & 6/12 2014
9/16 & 9118 2014
12/2 & 12/4 2014
313 & 3/5 2015
6/2 & 6/4 2015
9/15 & 9/17 2015
2/15 & 12/17 201
3/22 & 3/24 2016
7/6 & 7/7 2016
EPA Multiple
Dilution Series
CITY OF WILKESBORO POTW [NC0021717] WHOLE EFFLUENT TOXICITY RESULTS SUMMARY [BIOASSAY TEST]
June 1, 2014 through December 31, 2018
FEMALE ORGANISMS AVG/ Reproduction
SAMPLE #1 #2 #3 #4 #5 #6 #7 #8 #9 #10 #11 #12 FEM Mortality %Mortality RESULT % Reduction COMMENTS
Control
16
29
28
28
29
33
30
25
24
22
15
16
24.58
0/12
0%
3.7 % Eff
31
17
13
20
16
26
22
14
11
10
22
22
18.67
0/12
0%
1 PASS
1 24.07
Control
16
13
20
19
21
20
23
17
20
21
24
24
19.83
0/12
0%
3.7 % Eff
26
20
26
17
25
22
17
10
26
24
23
19
21.25
0/12
0%
1 PASS
1 -7.14
Control
21
24
26
19
23
22
25
26
23
18
20
23
22.50
0/12
0%
3.7 % Eff
21
25
26
28
25
24
20
28
25
28
28
20
24.83
0/12
0%
1 PASS
1 -10.37
Control
25
11
20
18
23
22
20
22
21
22
22
24
20.83
0/12
0%
3.7 % Eff
25
34
25
22
29
20
24
30
27
27
28
25
26.33
0/12
0%
PASS
-26.40
Control
22
21
19
15
17
25
20
17
19
13
27
23
19.83
0/12
0%
3.7 % Eff
22
25
21
26
17
27
26
25
20
25
19
21
22.83
0/12
0%
1 PASS
1 -15.13
Control
26
30
29
30
25
24
26
29
29
26
29
26
27.42
0/12
0%
3.7 % Eff
27
29
26
28
31
29
30
29
26
27
30
31
28.58
0/12
0%
1 PASS
1 -4.26
Control
1 16
21
1 23
24
26
25
25
26
24
13
26
25
22.83
0/12
0%
3 7 % Eff
1 27
26
1 25
30
24
27
27
29
23
27
17
27
25.75
0/12
0%
1 PASS
1 -12.77
Control
24
27
24
24
24
20
28
25
30
28
26
27
25.58
0/12
0%
3.7 %Eff
25
27
21
23
29
26
25
26
25
29
29
28
26.08
0/12
0%
1 PASS
1 -1.95
Control
18
25
23
26
18
22
24
25
28
25
23.4
0/10
0%
1.85% Eff
28
28
21
24
24
24
22
1
22
25
25
24.3
0/10
0%
-3.85
2.775% Eff
24
24
20
24
24
23
26
27
25
23
24
0/10
0%
-2.56

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9/4 & 9/6 2018
12/4 & 1216 2018
* not included in final results per Meritech
Control
29
25
24
28
22
23
24
31
29
24
26
26
25.92
0/12
0%
3.7 % Eff
29
26
26
25
27
27
23
28
26
26
29
24
26.33
0/12
0%
PASS
-1.61
Control
24
24
29
23
19
23
24
28
28
25
17
23
1 23.92
0/12
0%
3.7 % Eff
25
27
24
24
27
24
25
27
27
26
25
26
1 25.58
1 0/12
J 0%
1 PASS
1 -6.97