NC0066516_More Information Received_20190226

Hazen azen and Sawyer
Hazen
498 Seventh Avenue, 11th Floor
New York, NY 10018•212.539.7000
RECEI"VED/DFNR/DWR
Transmittal Letter
To: Copies: FEB 2 6 2019
Julie Grzyb
North Carolina Department of Environmental Quality, Water Water Resources
Quality Division Permitting Section
512 N Salisbury St, 9th Floor
Raleigh, NC 27604
From: Date:
Tim Devine, PE February 26, 2019
Subject:
Terrible Creek Wastewater Treatment Plant NPDES Permit Application No. NC0066516, Town of Fuquay-Varina
We are sending you:
® Attached ❑ Under Separate Cover Via the Following Items:
❑ Shop Drawings 0 Plans 0 Specifications 0 Change Order
❑ Prints 0 Samples 0 Copy of Letter 0 Reports
® Other: Terrible Creek WWTP NPDES Permit Application No. NC0066516, Town of Fuquay-Varina
NPDES Renewal Application Package Update— Part E Toxicity Testing Data
Copies Date Drawing No. Rev. Description Action*
1 2/26/2019 ---- Revised EPA Form 2A Part E Toxicity Testing Data FA
1 2/26/2019 ---- Attachment(Tab F of Oct. 2018 NPDES renewal FA
application package): Second Species Toxicity Tests
Action*
❑ A Approved 0 CR Correct and Resubmit ❑ Resubmit_Copies
❑ AN Approved As Noted ❑ F File ❑ Return_Copies
❑ AS As Requested ® FA For Approval ® Review and Comment
❑ Other:
Mailing Method
❑ U.S.Postal Service 1st Class ® Courier/Hand Delivery 0 FedEx Priority Overnight ❑ FedEx 2-Day Delivery
❑ Certified/Registered Mail 0 United Postal Service(UPS) 0 FedEx Standard Overnight 0 FedEx Economy
❑ Other:
Comments:
hazenandsawyer.com

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Terrible Creek V V TP, NC0066516 Renewal Neuse
SUPPLEMENTAL APPLICATION INFORMATION
PART E. TOXICITY TESTING DATA
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points: 1)POTWs with a design flow rate greater than or equal to 1.0 mgd;2)POTWs with a pretreatment program(or those that are
required to have one under 40 CFR Part 403);or 3)POTWs required by the permitting authority to submit data for these parameters.
• At a minimum,these results must include quarterly testing for a 12-month period within the past 1 year using multiple species(minimum of two
species),or the results from four tests performed at least annually in the four and one-half years prior to the application,provided the results
show no appreciable toxicity,and testing for acute and/or chronic toxicity,depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition,this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition,submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity,provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation,if one was conducted.
• If you have already submitted any of the information requested in Part E,you need not submit it again. Rather,provide the information
requested in question E.4 for previously submitted information. If EPA methods were not used,report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below,they may be submitted in place of Part E.
If no biomonitoring data is required,do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
complete.
E.1. Required Tests. Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
® chronic ❑ acute
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one
column per test(where each species constitutes a test). Copy this page if more than three tests are being reported.
Test number: 001 Test number: 002 Test number: 003
a. Test information.
Test Species&test method number Pimephales Promelas Pimephales Promelas Pimephales Promelas
EPA1000.0 EPA1000.0 EPA1000.0
Age at initiation of test <48 hours <48 hours <48 hours
Outfall number 001 001 001
Dates sample collected 10/22/18, 10/24/18, 10/25/18 11/12/18, 11/14/18, 11/15/18 12/3/18, 12/5/18, 12/6/18
Date test started 10/23/2018 11/13/2018 12/4/2018
Duration 7 days 7 days 7 days
b. Give toxicity test methods followed.
Short-Term Methods for Estimating the Short-Term Methods for Estimating the Short-Term Methods for Estimating the
Manual title Chronic Toxicity of Effluents and Recieving Chronic Toxicity of Effluents and Recieving Chronic Toxicity of Effluents and Recieving
Water to Freshwater Organisms Water to Freshwater Organisms Water to Freshwater Organisms
Edition number and year of publication 4th Edition, 2002 4th Edition,2002 4th Edition, 2002
Page number(s) pp. 53-111 pp. 53-111 pp. 53-111
c. Give the sample collection method(s)used. For multiple grab samples,indicate the number of grab samples used.
24-Hour composite X X X
Grab
d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection X X X
After dechlorination
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 15 of 24

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Terrible Creek VVVVTP, NC0066516 Renewal Neuse
Test number: 001 Test number: 002 Test number: 003
e. Describe the point in the treatment process at which the sample was collected.
Sample was collected: Final Effluent Final Effluent Final Effluent
f. For each test,include whether the test was intended to assess chronic toxicity,acute toxicity,or both
Chronic toxicity X X X
Acute toxicity
9YP performed.
the type of test
Static
Static-renewal X X X
Flow-through
h. Source of dilution water. If laboratory water,specify type;if receiving water,specify source.
Laboratory water Soft Synthetic Freshwater Soft Synthetic Freshwater Soft Synthetic Freshwater
Receiving water
i. Type of dilution water. If salt water,specify"natural"or type of artificial sea salts or brine used.
Fresh water Soft Synthetic Freshwater Soft Synthetic Freshwater Soft Synthetic Freshwater
Salt water
j. Give the percentage effluent used for all concentrations in the test series.
0, 22.5,45, 75, 90, 100% 0,22.5,45, 75, 90, 100% 0, 22.5,45, 75, 90, 100%
k. Parameters measured during the test. (State whether parameter meets test method specifications)
pH Yes Yes Yes
Salinity N/A N/A N/A
Temperature Yes Yes Yes
Ammonia N/A N/A N/A
Dissolved oxygen Yes Yes Yes
I. Test Results.
Acute: N/A
Percent survival in 100% 0 0 0
effluent
LCs0
95%C.I.
Control percent survival
Other(describe)
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 16 of 24

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Terrible Creek WWTP, NC0066516 Renewal Neuse
Chronic:
NOEC 100% 100% 100%
IC25 N/A N/A N/A
Control percent survival 100% 100% 100%
Other(describe)
m. Quality Control/Quality Assurance.
Is reference toxicant data available? Yes Yes Yes
Was reference toxicant test within Yes Yes Yes
acceptable bounds?
What date was reference toxicant test 10/23/2018 11/13/2018 12/4/2018
run(MM/DD/YYYY)?
Other(describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ® No If yes,describe:
E.4. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information,or information regarding the
cause of toxicity,within the past four and one-half years,provide the dates the information was submitted to the permitting authority and a summary
of the results.
Date submitted: N/A/ / (MM/DD/YYYY)
Summary of results: (see instructions)
Required NPDES toxicity monitoring is submitted quarterly to NCDWR
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 17 of 24

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Terrible Creek V V TP, NC0066516 Renewal Neuse
SUPPLEMENTAL APPLICATION INFORMATION
PART E. TOXICITY TESTING DATA(continued)
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points: 1)POTWs with a design flow rate greater than or equal to 1.0 mgd;2)POTWs with a pretreatment program(or those that are
required to have one under 40 CFR Part 403);or 3)POTWs required by the permitting authority to submit data for these parameters.
• At a minimum,these results must include quarterly testing for a 12-month period within the past 1 year using multiple species(minimum of two
species),or the results from four tests performed at least annually in the four and one-half years prior to the application,provided the results
show no appreciable toxicity,and testing for acute and/or chronic toxicity,depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition,this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition,submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity,provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation,if one was conducted.
• If you have already submitted any of the information requested in Part E,you need not submit it again. Rather,provide the information
requested in question E.4 for previously submitted information. If EPA methods were not used,report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below,they may be submitted in place of Part E.
If no biomonitoring data is required,do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
complete.
E.1. Required Tests. Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
® chronic ❑ acute
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one
column per test(where each species constitutes a test). Copy this page if more than three tests are being reported.
Test number: 004 Test number: Test number:
a. Test information.
Test Species&test method number Pimephales Promelas
EPA1000.0
Age at initiation of test <48 hours
Outfall number 001
Dates sample collected 1/7/18, 1/9/18, 1/10/18
Date test started 1/8/2019
Duration 7 days
b. Give toxicity test methods followed.
Short-Term Methods for Estimating the
Manual title Chronic Toxicity of Effluents and Recieving
Water to Freshwater Organisms
Edition number and year of publication 4th Edition, 2002
Page number(s) pp. 53-111
c. Give the sample collection method(s)used. For multiple grab samples,indicate the number of grab samples used.
24-Hour composite X
Grab
d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection X
After dechlorination
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 18 of 24

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Terrible Creek V VVTP, NC0066516 Renewal Neuse
Test number: 004 Test number: Test number:
e. Describe the point in the treatment process at which the sample was collected.
Sample was collected: Final Effluent
f. For each test,include whether the test was intended to assess chronic toxicity,acute toxicity,or both
Chronic toxicity X
Acute toxicity
g. Provide the type of test performed.
Static
Static-renewal X
Flow-through
h. Source of dilution water. If laboratory water,specify type;if receiving water,specify source.
Laboratory water Soft Synthetic Freshwater
Receiving water
i. Type of dilution water. If salt water,specify"natural"or type of artificial sea salts or brine used.
Fresh water Soft Synthetic Freshwater
Salt water
j. Give the percentage effluent used for all concentrations in the test series.
0, 22.5,45, 75, 90, 100%
k. Parameters measured during the test. (State whether parameter meets test method specifications)
pH Yes
Salinity N/A
Temperature Yes
Ammonia N/A
Dissolved oxygen Yes
I. Test Results.
Acute: N/A
Percent survival in 100%
effluent /0
LCso
95%C.I.
Control percent survival
Other(describe)
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 19 of 24

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Terrible Creek WWTP, NC0066516 Renewal Neuse
Chronic:
NOEC 100
IC25 N/A
Control percent survival 95%
Other(describe)
m. Quality Control/Quality Assurance.
Is reference toxicant data available? Yes
Was reference toxicant test within Yes
acceptable bounds?
What date was reference toxicant test 1/8/2019
run(MM/DD/YYYY)?
Other(describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ® No If yes,describe:
II�
E.4. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information,or information regarding the
cause of toxicity,within the past four and one-half years,provide the dates the information was submitted to the permitting authority and a summary
of the results.
Date submitted: N/A/ / (MM/DD/YYYY)
Summary of results: (see instructions)
Required NPDES toxicitymonitoring is submitted quarterlyto NCDWR
4 4
END OF PART E.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
EPA Form 3510-2A(Rev. 1-99). Replaces EPA forms 7550-6&7550-22. Page 20 of 24

TAB F: Second Series
Toxicity Testing Results

• Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date:11/1/2018
Facility: Fuquay Varina NPDES#NCOO 66516 Pipe#: County: Wake
Lqb rat : Meritech,Inc. Comments
Signature of Operator In espy 3sible Charge
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Water Sciences Section
Aquatic Toxicology Branch
Division of Water Resources
1621 Mail Service Center
Raleigh,N.C.27699-1621
Test Initiation Date/Time FVTC 3:55 PM Avg Wt/Surv.Control 0.924 Test Organisms
Eff. Re t. 1 2 3 4 r Cultured
d In-House
Control Surviving# 10 10 10 10 %Survival 100.0 7 Outside Supplier
Original# 10 10 10 10
Wt/original(mg) 1.016 0.890 0.967 0,823 Avg Wt(mg) 0,924 Hatch Date: 10/22/18
22.5 Surviving# 10 10 10 10 %Survival 100.0 Hatch Time: 3:00 pm CT
Original# 10 10 10 10
Wt/original(mg) 0.885 0.793 0.850 0.758 Avg Wt(mg) 0.822
45 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.780 0.877 0.847 0.710 Avg Wt(mg) 0.804
75 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 - 10 10 10
Wt/original(mg) 0.680 0.951 0.822 0.807 Avg Wt(mg) 0,815
90 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.716 0.998 0.794 0.698 Avg Wt(mg) 0.802
100 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.845 0.834 0.905 0.762 Avg Wt(mg) 0.837
Water Quality Data Day
Control 0 1 2 3 4 5 6
pH(SU)Init/Fin 8.02 17.82 8.17 / 7.99 8.08 / 7.90 8.15 / 8.14 8.27 / 8.04 8.26 / 7.86 8.22 17.86
DO(mg/L) Init/Fin 7.84 / 6.68 7.53 / 7.40 7.90 16.80 7.45 / 7.72 7.95 / 7.46 7.68 / 6.89 7.70 16.73
Temp(C)Init/Fin 24.4 124.0 24.9 / 24.6 25.8 / 24.8 24.3 / 24.7 24.2 124.8 24.4 / 24.2 24.1 125.0
High Concentration 0 1 2 3 4 5 6
pH(SU)Init/Fin 7.18 / 7.53 7.19 / 7.73 7.33 / 7.66 7.23 / 8.04 7.81 / 7.82 7.83 17.63 7.50 / 7.60
DO(mg/L) Init/Fin 8.28 / 6.88 7.85 / 7.45 7.79 / 6.88 7.91 / 7.60 7.95 / 7.24 7.59 16.85 7.74 / 6.67
Temp(C)!nit/Fin 24.5 124.6 25.0 / 25,0 25.5 1 24.8 25.1 124.3 24.5 / 24.4 24.9 124.3 25.3 / 24.7
Sample 1 2 3 Survival Growth Overall Result
Collection Start Date 10/22/2018 _ 10/24/2018 10/25/2018 Normal ri Fl ChV >100
Grab Horn.Var. ri Fl
Composite(Duration) 24.0 24.0 24.0 NOEC 100 100
Hardness(mg/L) 58 58 60 LOEC >100 >100
Alkalinity(mg/L) 35 41 39 ChV >100 >100
Conductivity(umhos/cm) 476 492 500 Method Steel's Dunnett's
Chlorine(mg/L) <0.1 <0.1 <0.1
p.at Receipt("C) 1.2 1.1 1.3 Stats Survival Growth
Conc. Critical Calculated Critical Calculated
Dilution H2O Batch# 1326 1327 1328 22.5 10 18 2.41 1.5785
Hardness(mg/L) 44 42 48 45 10 18 2.41 1.8557
Alkalinity(mg/L) 53 54 52 75 10 18 2.41 1.6786
Conductivity(umhos/cm) 201 206 224 90 10 18 2.41 1.8865
100 10 18 2.41 1.3475

Meritech, Inc, (Lab # 027)
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Client: FVTC #of Organisms per Chamber: 10 Start Date: 10/23/18 Time: 3 s4
NPDES#: 0066516 Test Vessel Size: 400 ml End Date: 10/30/18 Time: J?. 0.7- ivl
Initiated by: (i7� Test Solution Volume: 250 ml
Date/Time Fed: 10/23/18 1. 36 ,^1 Temp. of Stock: 2 Randomization: 430 No
Date/Time Born: 10/22/18 3:00 PM CT #of Reps: 4 Incubator#:
Organism Source: Aquatox,Inc. Test Organism: Pimephales promelas Analyst(s): MR, CD, LV, SB
Transferred by: Fed by:
Date Time Initials Date Time 11 Initials Time 2 Initials
Day 1 10/24 )1 `UD.,v, LAD/LA) Day 0 10/23 (4r3Cp C1
Day 2 10/25 `/ /r VC43 Day 1 10/24 7 rSJ�,r2 'Mr? L.../
Day3 10/26 („j'Lr0 Day2 10/25 grit/he( 61�
Day 4 10/27 -�3 Day 3 10/26 r.� j�,�,.� j,, g'o4:.1
Day 5 10/28 ' Ol e•-k" "Zl Day 4 10/27 �/ (�� "2): yC-F1`'`
Day6 10/29 Zji.41 CA..1 Day5 10/28 C/z) /�,� $3
Day 6 10/29 /4-tvy‘ /1/).\_ :2)23 -
Test Termination Data: /
Initials/Signature: ` ,"41 v"
End Date: , 0/30/18

MER Ems...1wi. A / I m..,
ENVIRONMENTAL LABORATORIES
A OIvIs/on of Wetter Technology and Contra/s, /r.c.
Chemical and Physical Determinations
Client: FVTC NTPDES#: 0066516 Start Date: 10/23/18
End Date: 10/30/18
Day
Concentration: control 0-I 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
Soft Synthetic Feshwater
Batch tl: ) -(g r� i30 '"7 , 3)-1
pH: Initial F. ()2 ' D g t d 5 2
Final t.()? `f �U �. ! g uN-1 �-7r
D.O.: Initial d 11 �� 2 ci 4 �i{)S' /'Vt taL� n?-o
Final 6,id) —3Lb 6p .7 7a .'1/tp c 'fl U131
Temp.: 2 tt, 1'(,21 o<St8' 21.1
Final 2 Li, () �� �y '� � o?• (( /_ -76 f.?
Conductivity: Initial 1 1 c2-0'1 )blp 7 f j )0
Final I M uc I c2'c
Residual Chlorine: z©,t Z.0. j Gp•t L.b t /
Hardness: LJ q 4). 1 . -
Alkalinity: 5 61-
Day
Concentration: 22.50% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial —7, ?O �p "7. 7 ' T - �t.17 it
t.`' '! !
Final 7 fl- W T,5 '� �l 8r Ob 77 {3 f
I r
D.O.: Initial 7 c(o LO �1 7- 97 . iO'- 7,
Final G ivp2� 4f ,t 7 `7). 7sj (0,39 (01 en)
Temp.: Initial g-t- ac`�• 3 `�5 ) f
Final 02 l{
a�K.` ,i T5� �- dk,g L`�'� .;K,� 2
a s
Conductivity: Initial Q� g'4-1 (,g , � rj r
Final )j3 a ] ! ( .3 9.13.
Residual Chlorine: Z p: j 4-0.1 G O. i c: / 46,
Day
Concentration: 45.0% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial 7. (1 7/ LO L9C) . ,d 7 8'D j
Final ] 77X L lQ ,12
D.O.: Initial '-7,I • . C/3 S
Final �I7 -1 93g .�Di`�"t `7�f n1 ( -
1l,,�� �l � � G , ty
Temp.: Initial c9_l+3 �5► 5 ,Z5.7 a1 t'(r) �,?`� �,`( .5
Final a K g -�2`1'a 2-5,I A.1:\., L ZCi.(p
Conductivity: Initial j a 33 6 3q 0 3S ',3o.
Final 10 �UW 4 311 3 3w3 33ci
Residual Chlorine: G 0.f z c . ti GO. p. I CO, C c p, I 40,

ME W'rEiCW, :i is
ENVIRONMENTAL LABORATORIES
a[�VA/���m/N�n r�r Tae/ii.u%uVY i�neY t`.'nnere/t, /lac.
Chemical and Physical Determinations
Page 2 of 2
Client: FVTC NPDES#: 0066516 Start Date: 10/23/18
End Date: 10/30/18
Day
Concentration: 75% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial 7r49 _77 7 ci.5 7 13
P "�
Final 4(0 -7 /0 7 ) gift �q tDC1 10G
D.O.: Initial p,Oa _ i) ppT/ 4' 7, 10 B
I Q
Final L 77 ,E (o J R"q 712a ( - (83-.5
Temp.: Initial 914,'"), 11- ,f,a. r�]�1./ 9,q pi
Final g�,9 p��; 6 i 17,5 �q.�" l gq,�
Conductivity: Initial ?,C)Bpp .�o 1k
L4 0 �r� 'LI LT L�`3+U G I D
3 Final ' ' t +1})? Li
(} `-!_ g U` 0' 11
Residual Chlorine: ( L ,1 w. j Lo. t GO.( LO,C Z.,91
Day
Concentration: 90% 0-1 1-,2! 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial })1 7)aq 35 '133 '? S :-MCI 7151
Final _U� �� i0�� 7M2 -7?"( t='.�� _
D.O.: Initial S,L�, "W11 � 7,toe( �l {D� I
Final (t3-4 'yiv cc, 3t03 3ic, )g (640l2
Temp.: Initial 45 3 w9 ��;�� `v, r9-1.1•c; 948 pas i
Final 9 0 9•S ;114•1
Conductivity: Initial IN( y 11 4 .\ 4( 4 L (ps L Lei(0-
Final ;3 '�- ( 3 L1 70 L 39 ills) H611
Residual Chlorine: 40, t 40 ( 2.0,1 G O, t gyp,( er'') t r
Day
Concentration: 100% 0-1,, 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial h)U --71 )C-t ?)3 7Os7$1
Final 763 73 1 (pip gi 0ti 7)g) 7,tc, coo
D.O.: Initial qIl 1) 4
Final 618 (&5 (oitir< 7)'100 ?0-9 tot Sc
Temp.: Initial 9-4 5 .510 9_5 5 )',j 1 c r-! csi 3
Final ;II,ip r I3 .3 ,lk.1,l1 aU aII
Conductivity: Initial Li 7-3 ci lj 9 to
Final ti o< gels 4 q ' (00 s ' 1-
Residual Chlorine: Z ( LO L..o.( Coll LO.! GO,) G D•
Hardness: v��
Alkalinity: 34 j 3

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 10/23/18
End Date: 10/30/18
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesdayd
Rep A 0 � V _� �� 0 ()
Rep B 0 0 `" o 6
Rep C 0 0 _ ___0__
0 6
0 r>
Rep D 0 a % 0 �/
Concentration 22.50%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Re 0 P 6
p A 0 —0— ---0— �—
Rep B 0 v 11 41_1 0
Rep C 0 (/ 0 0 D
Rep D 0 ___ _ ____ 0 _ U U 0
Concentration 45.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep 0 0 _0_ 0 V (d)
B 0 , — 0 V �"
.)
Rep - __ --
Rep C 0 0 ___0-_ 0 0 0 0
d7 Rep D 0 —9-
6L�

•
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 10/23/18
End Date: 10/30/18
O I
Concentration 75/o
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
..11 , v 1
RepA 0 _ U
'
0
RepB 0 �_ _a_ �)_ .� _
Rep 0 0 C� 0 v 0
Rep D 0 J�l 0 0 0 G 0 __
Concentration 90%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 / 4
Rep B 0 (0 ____0_ 0 G _ 0
U
—0—
RepC 0 —0_- 0 t) ___ _a_ 0
Rep D 0 _CL ) U OC) _
Concentration 100%
Tuesday Wednesday Thursday Friday Saturday Su ay Monday Tuesday
Rep A 0 ---0— Li --0— _____a_ ____0_
Rep B 0 Oo _0_ 0 D o V
Rep 0 ____40 D
..4..._ _o__
Rep D 0 E) _ QU0 1

AMERI TECH, INC.
ENVIRONMENTAL LABORATORIES
A D of Water Technologyand Controls,Inc_
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC Page: 1 of 2
NPDES #: 0066516 Outfall:
Date of Test: 10/23/18
Initial weights taken on: /- 6-- /jc by: i--1./ Organism: Pimephales promelas
Final weights taken on: 0- 3(-(7 by: CA"
PAN WT. PAN + ORG. WT. OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) #ORG. WT./ORG. (mg) SURV%
A gQ /41,6 A. 31 /0, /6 /0 / o/6 roe)
B g.29, q F3flWzi Fi g° /0 Oi a4'O /0o
0
c E13. O g. 34'17 q, 67 /v 0, q67 fr O
D ?!L1. gq )I ,Q F 3 10 0! Q3 /(iii
A I ?Q6, L-17 S736, K Y Id 0, FR5 (oo
22.50% B F/�. Oo F(25, 613 71 q3 le 0 793 lD()
c g1703 F?65O g So /0 a R6o l00
D gaq, I a F36 7( 7, 3? /0 ,n176? /0(7
A 17. ( 7_ 026,q7 7 E<3 /0 Q 7F0 /0 )
45.0% B El--7, -66 e96, 63 F 77 /0 0. , 77 /Qv
c wQF57 g37Oq w, q7 lD o1Fq7 /(-)0
o P--1300g6-0, 10 7 10 /0 a- 710 161

MER/TECH. INC.
ENVIRONMENTAL LABORATORIES
�_ A Division of W.t r Technology and Controls.lnc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC Page: 2 of 2
NPDES #: 0066516 Outfall:
Date of Test: 10/23/18
Initial weights taken on: '- 5—)g by: (A✓ Organism: Pimephales promelas
Final weights taken on: /o-3/-l1 by: (A)
PAN WT. PAN +ORG. WT. OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) #ORG. WT./ORG.(mg) SURV%
A &' 5c .3 I5 k3 0,to370 l00
75% B F31 , 3 fI 5J 10 o qci
, Qc_Q /0 0( FQ /an
D F-V 97 07 /0 O- ?() .7 /a )
A ?N4 e5-- 7i /( ) 0 a 7/( /o0
90% B 0Q6, W3(t /4 cf qq?
c17� y�( �s �, 3. 7, q -( /0 0 f 7 t( 1 t962
D 153 I•Ci 6 ? , /0 0.6 ? lira
A go..0(,) g2e, q6' E! z6' In oFg5 lea
100% B b P6 64'3 , F, 3e/ / (' 36/ /Oo
c gQ6e of R 35e 13 qt 0S y o O4o5 /or .

MERITECH, INC. Meritech Sample ID#: Iwef.%Vc
Bioassay Sample Chain of Custody
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: Tad)vk_ of- Irk t) ( VV-c �t, � PO#:
Contact Person: Pam-- S('.el tkv..rr)vv _ NPDES#: NCOOCaCirt ce
Address: 5)t22 (•f.cc i ) f.Z Phone:
City: fi!¢vti.y Pipe#:
County: Ck ..cr State: NC— Zip: ZZSZCp
SAMPLE INFORMATION
Sample Site: Te-rr+btG C<;LIC d.c.--
Sample Type: ❑Grab 0 Composite #of containers: Z
Sampling Time: Start Date: Cc)•-IL'tY_ Start Time: Wei) ATi PM
End Date: (J'2--', -L'{ End Time: O`t:,,•J A.t PM
***Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be<6.0°C upon receipt t the laboratory'
Collector's Name: Print: e-tr,S 14-(6._''� Signature:
TOXICITY TEST INFORMATION
Test Required: ,Chronic(7 days) Test Organism: sigl Ceriodaphnla dubia (water flea)
❑Acute(24-48 hours) 1J Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: 60 % Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION l
Relinquished by: Date: /Q 4 !J)3 1 Time: f•f 3 0 8 PM
Received b j...)=......"---- Date: (6 •-c --Ij S- Tin:0 /( , 3 0 • .I PM
Relinquished by: Date: /d — Time: }' 6, PM
Received by: Date: Time: AM PM
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Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX 154 Meritech Pick-up ❑Delivered ❑ Other
**Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED'"
St Is-gl- SAMPLE RECEIVING (Laboratory Use Only)
relinquished by:
J
Received by: Date: � '�}31f;} Time: 33 O AM 64
Sample Temperatures("C): F1 f d i r?-7 I Sample Cunditiun. �
WHITE= Laboratory copy YELLOW=Client copy

MERITECH, INC. Meritech Sample ID#: ,u)�(i3
Bioassay Sample Chain of Custody
•9 642 Tamco Rd,Reidsville NC 27320
�� Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike.reed(ilmeritech-Iabs.com Web Site: www.meritech-Iabs.com
CLIENT INFORMATION
Client: f)w..ti G.- r J co./i
(.00(A:) 1 PO#:
Contact Person: 11)'�„ac- St'kctw1 NPDES#: Na.VC 2i t l.ci
Address: •s`ri tl (i L(V't5(' (7-6- Phone:
City: Foguis-j Pipe#:
County: WA k t- 1 State: iti c.._ Zip: 2-7s 2 w
SAMPLE INFORMATION
Sample Site: T if•Ite-Cii-ct/ (,JLJ t L &coin... r
Sample Type: ❑Grab VI Composite #of containers: Z
Sampling Time: Start Date: I -2,'-r"t 41 Start Time: Cit-u) Ali
PM
End Date: (0'-2-C-t SS/ End Time: (1" ' P PM
"*'Triple rinse sample container with sample before filling.Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be<6.0°C upon receipt at the laboratory***
OCollectors Name: Print: G y V t.rt"- Signature:
TOXICITY TEST INFORMATION
Test Required: XChronic(7 days) Test Organism: ❑Ceriodaphnia dubia (water flea) 1
❑Acute(24-48 hours) (j,,Pimephales promelas (fathead minnow)
❑ Mysidopsis bahla (shrimp)
IWC: -/U % Test Concentrations(if multiple dilutions);
Comments:
SHIPPING INFORMATION
Relinquished by: . 4:11 Date: (U-Z5- ($ Time: /r /5 62PM
Received b • /� Date: f 0/2.,s /i Time: // Y s 0 PM
I Relinquished b ' Date: !OAP..571,9 S? Time: 16-02.5� AM 0
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX (,Meritech Pick-up ❑Delivered [] Other
**Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED**
SAMPLE RECEIVING(Laboratory Use Only)
Anguished by:
Received by: Date: WI'/ b Time: COD'S AM
1 Sample Temperatures(°C): I, / l t 1 I I Sample Condition: _ �___
WHITE=Laboratory copy YELLOW=Client copy

MERITECH, INC. MeritechSampleIDN: \01 k,t �
Bioassay Sample Chain of Custody
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike.reedAmeritech-Iabs.com Web Site: www.meritech-iabs.com
. (( CLIENT INFORMATION
Client: I K 0 j Uq( ( ..)T? PO#:
Contact Person: f7cL.• Sf ti<:L.�U� NPDES#: NCO-AA: tb
Address: f i Z (-,I t}92 j1 Phone:
City: f Jci. Pipe#:
County: (..)l'}d/L1 State: Zip: W'SZ
SAMPLE INFORMATION
Sample Slte: ktrhk. ("Ail.AS(l7
Sample Type: ❑Grab c46 Composite #of containers: 3
Sampling Time: Start Date: (0-ZS t1 Start Time: !CA5 Ayl PM
L
End Date: 10-2 i,if End Time: iY('J Arty' PM
***Triple rinse sample container with sample before filling.Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be<6.0°C upon receip t the laboratory***
Collector's Name: Print: e11/ > )�i (l&✓� Signature: —
TOXICITY TEST INFORMATION
Test Required: [A Chronic(7 days) Test Organism: ❑Ceriodaphnia dubla (water flea)
El Acute(24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: �} % Test Concentrations(If multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by: Date: /D/p2 6 Jt 8 Time: 42_1/O AM
Received by: - —° Date: /O/076//o Time: JoZ..VO AM
Relinquished b Date: f 07p.._64g, Time: /S O-S AM cPM
Received by: - Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX 7" Meritech Plck-up ❑Delivered ❑ Other
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED**
SAMPLE RECEIVING(Laboratory Use Only)
elinquished by:_(
Received by: Date: t 4'314./t Yj Time: ER.r AM ii J
Sample Temperatures CC): L ` 1 1, /f ' J / Sample Condition: (6
WHITE=Laboratory copy YELLOW=Client copy -0111111MIIIIMNIMI

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Shapiro - Wilk' s Test for Normality
D = 0. 1518
W = 0 . 9743
Critical W = 0 . 8840 (alpha = 0 .01 , N = 24)
W = 0. 9160 (alpha = 0 . 05 , N = 24)
Data PASS normality test (alpha = 0 . 01) . Continue analysis .

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Bartlett ' s Test for Homogeneity of Variance
Calculated B1 statistic = 3 .3216 (p-value = 0.6505)
Data PASS Bi homogeneity test at 0. 01 level . Continue analysis .
Critical B = 15 . 0863 (alpha = 0 . 01, df = 5)
= 11. 0705 (alpha = 0 . 05, df = 5)

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
ANOVA Table
SOURCE DF SS MS F
Between 5 0 . 0424 0 . 0085 1. 0065
Within (Error) 18 0 . 1518 0 . 0084
Total 23 0 . 1942
(p-value = 0.4422)
Critical F = 4 . 2479 (alpha = 0 . 01, df = 5, 18)
= 2 .7729 (alpha = 0 . 05, df = 5, 18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0 . 05)

Title : FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Dunnett 's Test - TABLE 1 OF 2 Ho:Control<Treatment
TRANSFORMED MEAN CALCULATED IN SIG
GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0 . 05
1 Control 0 . 9240 0 . 9240
2 22 . 50 0 . 8215 0 . 8215 1. 5785
3 45 0 . 8035 0 . 8035 1 . 8557
4 75 0 . 8150 0 . 8150 1.6786
5 90 0 . 8015 0 . 8015 1. 8865
6 100 0 . 8365 0 . 8365 1 .3475
Dunnett critical value = 2 .4100 (1 Tailed, alpha = 0 . 05, df = 5, 18)
Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Dunnett ' s Test - TABLE 2 OF 2 Ho:Control<Treatment
NUM OF MIN SIG DIFF % OF DIFFERENCE
GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL
a Control 4
22 . 50 4 0 . 1565 16 . 9 0 . 1025
3 45 4 0 . 1565 16 . 9 0 . 1205
4 75 4 0 . 1565 16 . 9 0 . 1090
5 90 4 0 . 1565 16 . 9 0 . 1225
6 100 4 0. 1565 16 . 9 0 . 0875

Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date:11/28/2018
Facility: Fuquay Varina NPDES#NCO() 66516 Pipe#: County: Wake
',gle tort': Meritech, Inc Comments
Signature of Operator in Responsible Gharge
J
Signature of Laboratory Supervisor
1A1L ORIGINAL TO: Water Sciences Section
Aquatic Toxicology Branch
Division of Water Resources
1621 Mail Service Center
Raleigh,N.C.27699-1621
Test Initiation Date/Time 11/13/2018 4:25 PM Avg Wt/Surv.Control 0.779 Test Organisms
%Eff. Repl. 1 2 3 4 lu Cultured In-House
Control Surviving# 10 10 10 10 %Survival 100.0 r Outside Supplier
Original# 10 10 10 10
Wtoriginal(mg) 0.850 0.697 0.769 0.801 Avg Wt(mg) 0.779 Hatch Date: 11/12/18
22.5 Surviving# 10 10 10 10 %Survival 100.0 Hatch Time: 3:00 pm CT
Original# 10 10 10 10
WUoriginal(mg) 0.791 0.632 0.748 0.767 Avg Wt(mg) 0.735
45 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.608 0.651 0.739 0.768 Avg Wt(mg) 0.692
75 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.714 0.736 0.706 0.743 Avg Wt(mg) 0.725
90 Surviving# 10 10 10 10 %Survival 100,0
Original# 10 10 10 10
Wt/original(mg) 0.717 0,687 0.693 0.752 Avg Wt(mg) 0.712
100 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.679 0.708 0.667 0.733 Avg Wt(mg) 0.697
Water Quality Data Day
Control 0 1 2 3 4 5 6
pH(SU)Init/Fin 8.03 / 7.99 8.15 / 7.96 8.07 / 7.73 8.13 / 7.97 8.13 / 8.07 8.22 / 7.92 8.11 / 7.69
DO(mg/L) Init/Fin 7.88 / 7.36 7.79 / 7.73 7.87 / 6.75 7.62 / 7.83 8.05 / 7.50 8.17 / 7.34 7.69 / 6.55
Temp(C)Init/Fin 25.1 / 24.1 24.5 / 24.5 24.7 / 24.6 25.0 / 24.3 25.0 / 24.5 25.4 / 24.5 24.0 / 24.6
High Concentration 0 1 2 3 4 5 6
pH(SU)Init/Fin 6.95 / 7.65 7.12 / 7.70 7.17 17.63 7.37 / 8.04 7.92 / 8.07 8.05 / 7.78 7.50 / 7.71
DO(mg/L) Init/Fin 8.18 / 7.44 8.04 / 7.18 8.22 r 7.00 8.19 / 7.85 8.12 / 7.47 8.03 17.20 7.92 / 6.45
Temp(C)Init/Fin 25.7 / 24.6 25.5 124.7 24.8 / 24.2 24.8 r 24.7 25.9 / 25.2 25.3 / 24.7 24.8 124.9
Sample 1 2 3 Survival Growth Overall Result
Collection Start Date 11/12/2018 11/14/2018 11/15/2018 Normal TI PI ChV >100
Grab Horn.Var. TI PI
Composite(Duration) 24.0 24.0 24.0 NOEC 100 100
Hardness(mg/L) 54 50 48 LOEC >100 >100
Alkalinity(mg/L) 51 56 56 ChV >100 >100
Conductivity(umhos/cm) 479 384 399 Method Steel's Dunnett's
Chlorine(mg/L) <0.1 <0.1 _ <0.1
p.at Receipt(°C) 1.6 1.4 1,6 Stats Survival Growth
Conc. Critical Calculated Critical Calculated
Dilution H2O Batch# 1332 1333 1334 1335 1336 22.5 10 18 2.41 1.1986
Hardness(mg/L) 44 42 44 44 44 45 10 18 2.41 2.3502
Alkalinity(mg/L) 54 53 52 55 48 75 10 18 2.41 1.4597
Conductivity(umhos/cm) 209 185 200 195 187 90 10 18 2.41 1.7945
100 10 18 2.41 2.2096

Meritech, Inc. (Lab # 027)
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Client: FVTC #of Organisms per Chamber:10 Start Date: 11/13/18 Time: of i Q
NPDES#: 0066516 Test Vessel Size: 400 ml End Date: 11/20/18 Time: / 0. 1)
Initiated by: ,/y12\./k_i Test Solution Volume: 250 ml
Date/Time Fed: 11/13/18 o�`vUpr k Temp. of Stock: a2S. J °C Randomization: 0 No
Date/Time Born: 11/12/18 3:00 PM Cr #of Reps: 4 Incubator#:
Organism Source: Aquatox,Inc. Test Organism: Pimephales promelas Analyst(s): MR, CD, LV, SB
Transferred by: Fed by:
Date Time Initials Date Time 1 Initials Time 2 Initials
Day 1 11/14 l/;05;4ri LP/64t/ Day 0 11/13 0-,0Upri L.-" (9 0(flri r,-Ag •
Day 2 11/15 :a)" '`/,J Day 1 11/14 7 ��ja,.� / / 3:I j p
Day 3 11/16 0 a4„?.1 ,/i'/i'�/ Day 2 11/15 (' 5&Ail- G 0
Day4 11/17 W.o.. S-4? Day3 11/16 ',57�r„� t -" 'C 70pM t
Days 11/18 1,7 -Cj(oki^^^ Day4 11/17 ;�S�r+ ,X •f f)peit tA.
Day 6 11/19 l r 5s„�} �,�1 Day 5 11/18 -3 v� 3= d�f^ t
Day 6 11/19 a"/Oh/4- fSL- ;�)Mr-
Test Termination Data:
Initials/Signature: itka-- 2712-tecei CA.14y(j i�.0'-2-, �,1vaxL,
End Date: 11/20/18

y MER 1 TECH, 1 Al C.,
ENVIRONMENTAL LABORATORIES
'C721401FA Pfvisfon of Water technology and Controls, Inc.
Chemical and Physical Determinations
Client: FVTC 1N1PDES#: 0066516 Start Date: 11/13/18
End Date: 11/20/18
Day
Concentration: control 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
Soft Synthetic Feshwater/ 3 aC ) 1 r S c . /3.3 6,'>
Batch#: � � t � � 33
pH: Initial X 03 gi is U•-0-7 j3ti v53/3 (--f e) U
Final .) e1 I(" _ �3 7, l 7- Q,-,- 7 7 3- coCol�
D.O.: Initial 7 ik 37 1(4�C� _ x'O�) ,1`, -1
'
Final 1( D 3✓' f e ,7�27 7.5d 7,3..4 . 6,cs
Temp.: Initial �S. 1 2 y�S `7 05
ct t) � ‘1 r2 t(,-0
Final t'l", 1 2Lj S 711. I., �'/`1 (2 v- .4A 2p �( ,(,,
)
Conductivity: Initial p 'ivt f)1 Lk I g;' � y ) FG , `�f}\\ / 37
Final I�"I, a i , I( ' (9 me\ a-1 gto(
Residual Chlorine: 7.O,( z. .( GO,f GO,1 2-0,( --Co• I e--6.(
Hardness: Lj 7 Cy i( if 9 - ,. Li
Alkalinity: rj ty — ?a 5g..., 5S -�
tlY
Day
Concentration: 22.50% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial (r 67 ?t j?-- 7,7q g. O T '17 C1
Final 1W Gf -met (-70
D.O.: Initial 7 iF4 7 LI6j S ? cg 0 i 2-'I -
"_
Final Iv i et.).) To ?,'/ 7. .� 9 �' -1 i - (.P
Temp.:
Initial a,--J aS, 1 7-c{,Li 92L •�( '4 ,(
'
Final 24,( ')t1'o�L alfr) na ,2' - -ki,.c( *5'.-
Conductivity: Initial ). j 231 1-f 3 P' t 1 ),`1 1 '7 1-(5
Final to/i ,, ft(a ;25� g.5 q 1G0 x,e a.443
Residual Chlorine: L(9, 1 t.0 r( L.01 ( /-0,1 Z--OrI L.O,.l L0,/
Day
Concentration: 45.0% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial --7,--, -3(4� 7 S3 'Ito-4 ...0-J ,'/f Final r3 . 9?. 7() 666 ° ) ,(Dq
D.O.: Initial , 4 - ti J() F..0 3 ,.i 3
Final 73' ' 3(p _(p,(173 -ZF1 7,57 7)l -3 Lot-t
Temp.: Initial . LA Q-S, f 24.) 2-, 3 4.5>) 2 \'( 9,"4 i
Final (�qt ,to )) --,? � •0 2tt. o at(, ci
Conductivity: Initial -3a 7 '�-'�333 'ra 7 7 Q,?0 1-4(7 97
Final ')t (1 3 quo '3 o 1 1 / 30-) 3o
Residual Chlorine: LO.i Z.-6.1 GO,1 L.0,l 1__O,( L. ,1 GQ, 4

AWE R/ CH, Alf C,
ENVII N 1 1'JTAL LABQR.-r IES
A ON.a/un:nt Nan Mr 7-4.chttulawiw idn€F Cnn n o/s. Inc.
Chemical and Physical Determinations
Page 2 of 2
Client: FVTC NPDES#: 0066516 Start Date: 11/13/18
End Date: 11/20/18
Day
Concentration: 75% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial —71/� 04-1 7. kk q6 ` 07 (Q
Final 77 3 X R-,r�7 60 -1�770 .
D.O.: Initial 0<, p(� 7cf ? g/i 1
Final ( ( 7. 7-7 `7,5-0 1 (049
Temp.: Initial �.�. 26 Zti Li 2'• act C) aZ.Li 1 t '2 11, 6
Final a�• 0 2 'x�.7 ,1 �`1 ' � ���. t K• ?�
Conductivity: Initial 3 G 5 t{()Li 3akf 33.-5 3 Li 3 3%-ci •3 ��
Final Lie 1113 361 -7)6, 3C0-1 ) 66,
Residual Chlorine: Z Q. t La ( L•O#( GU:1 L 0,t LOt( CZ.)• 1
Day
Concentration: 90% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial 7, 0 7 j l 7 /6 7,3y 1, -5 O 6 g6
Final ( — ?)Q01 74c
D.O.. Initial -- T f z q.04 7-06 %- O 7 71 in-
Final 3 go i -7N 7, ( .3?"
Temp.: Initial g4 D-t u 12 k tic
Final off(,." 9M 7 I(A 9-4 3 ay• 1 a Lt `f
Conductivity: Initial L 14 L (j 36 36 j3 373
Final IO g 3 i-76 og ve3.3
Residual Chlorine: Zat 1 LC'// 40 t !_O r 1 l GI E C-O I J LO.1
Day
Concentration: 100% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial G. ci S `),1?. 7, /7 ? 7r y 7C) -7c6)
Final (D5 . "' ) S 1 i3 U� b•0 7 �y Tl'J
D.O.: Initial 7 j g ,�� r2 ! l� � b t D3 // tp-
Final 3(cj ry 7 7-a5 747 v '� 14�45
Temp.: Initial .QC,."'1 �� 5 2-t ' 2�.Cj �S�G1 ,2S.3 o�t(,t�
Final 9 Li• lv �N 7 ` 4( � /� t�S-� a�1t� 2
Conductivity: Initial Li`7' two 3?1 3 q q Lion
Final 16 1 95 L/06, ci3OQ `- 4) cos Hd.,3
Residual Chlorine: Lo.1 ZZ:).t GOI( Li). I LO, 1 La,)
Hardness: 54 50 • f
Alkalinity: 5.1 56

1
MERI-TECH INC. •
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 11/13/18
End Date: 11/20/18
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
RepA 0
a 60 Q
0
ll��
Rep B 0 v C) —0— c) 0 _
p (�
Re C 0 0 U /� u _
0 /�.
rJ�
�`
Rep 0 D U C � C 0 _ 0
Concentration 22.50%
Tuesday Wednesday Thursday Friday Sat rday Sunday Monday Tuesday
Rep A 0 0 06.) 0 __O____
Rep B 0 6 0 o C) ��
RepC 0 �) U _ l� 0 0 0
Rep D 0 _Ai_ __O__ 0 l J 0 0 —IL
Concentration 45.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
1
Rep A 0 -6— —� —0-- -- --4— _179-____
Rep B 0 0 ___Q—
6 0 0 _O____
p 0 Re C 0 0 l 6
-0 (-)Rep D 0 — 0 0 0 0 ____0_ _0_

MERI-TECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 11/13/18
End Date: 11/20/18
Concentration 75%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 0
( l 0 — 0
Rep B 0 6 l 0 0 r
C
Rep C 0 D (J --tom 6 Cr) - 0
0
Rep D 0 0 --1j— —C— o
" 0
Concentration 90%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 j 0
0
RepB 0 11 v 79 6) V ril 0
D ,..,
RepC 0 ( ) _— __ 0
Rep D 0 1/ 0 Oc) p 0
Concentration 100%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
v
RepA 0 / O C)
CY ____6_ __D___
Rep 0 J -1)-_ -�fV-- V 0
r
6
RepC 0 __ _ _ n 0 0
Rep D 0 b 0 0 0

MERI TECH; INC_
ENVIRONMENTAL.LABORATORIES.
A Devlsion pf Water-Trrctino%yy and Concc915,,„c
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC Page: 1 of 2
N PDES #.• 0066516 Outfall:
Date of Test: 11/13/18
Initial taken on:weights //` b : (A/ Organism: Pimephales promelas
g � ^ � Y
Final weights taken on: // - a('VV by: (,L"
PAN WT. PAN +ORG. WT. OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) # ORG. WT./ORG. (mg), SURV%
A W30.to c-'.-3?? 0 o ( r(V 5° Loo
B QO. 7 . F97, q / 0 0 ,6? 7 too
0
c E37 a 741 10 0.766( too
D ge/,30 3P.31 w• of /0 0130/ loo
A 8(9X to g311. 0 4I /o 100
22.50%
B go . /r g/Q, z6 6 ,3g / o oh 2 too
c pz-4( 7 a I/ -7, In 0.7LiF 100
D 'WIq 5'q R27 76 l . `o oI 76 7 ion
A F/6, LIE g QS3 6 , ox /00 0160e 100
45.0% B o(;)i W39,70 6 , 6/ i o G, s/ lou
c ?g,?4,e5 l o oI 73q 100
D g 5gt-( 39 . g5. 76y 10 d7( 100

MERI TECH,.. INC..
' ENVIRQNMENTAL:.LABORATORIES
A Diids�e,t c/'Water Te vn-,, yy..a,td CgntrpI,/Zr
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC Page: 2 of 2
NPDES #: 0066516 Outfall:
Date of Test: 11/13/18 Q /
Initial weights taken on: l /-9J/o by: (A/ Organism: Pimephales promelas
Final weights taken on: /1 )- l? by: (A)
PAN WT. PAN + ORG. WT. OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) #ORG. WT./ORG. (mg) SURV%
A 7a6., �o Y3,- 0o ' 7' I 1 I v o. 7/ /0(O
75% B Zo t. l c g •,6 71 36 >'. 0 a 73 / 0��
_ c F26-0,/ E%I33 7o70 t0 0.1706 / 0
D F�7 7o W35‘ /3 71/`3 /() O, 7L/3 100
A <<3 . Wgggg Z / 7 10 a, 7/7 /00
90%%o B FA,56 Fggi ,-/ 6,T7 to (), (07 h-X)
c EgX FL 356 6 43 ( 0 c,6 3. / 0 )
Q01 (3OL 53 75Q l 0 0176a. /00
A F3/a1 •F3 ( _ 6 ' 7q ot(07 1000
100%%0 B $1,2�7C� gig.7F 70Y l0 0, 706 f 0�
c 89,t3q F36.% 6.67 /0 U, 6 7 /00
D ? .�� W 1-/,, 77 -7 33 / () 0 73'3 10

MERITECH, INC. Meritech Sample lD#: `t
Bioassay_Sample_Chafn_of_Custady
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: o,,n of Foot oy — VotOrlq Pa#:
Contact Person: NPDES#: NC 006CS-1C
Address: Phone:
City: Pipe#: 00/
County: State: Zip:
SAMPLE INFORMATION
Sample Site: l 2- k!_(Ve T-
Sample Type: ❑Grab Composite - #of containers: Z
Sampling Time: Start Date: ] ( -(Z--(' Start Time: p$bc) 6. PM
End Date: !( ^ (3 -(c? End Time: 09crcT a PM
***Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be<6.0°C upon receipt at the laboratory***
Collector's Name: Print: Ilpy ��� Signature: 71`4/
TOXICITY TEST INFORMATION
Test Required: Pf Chronic(7 days) Test Organism: ❑ Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) (71 Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: 9v % Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by: � Date: Jl —/3 r{/Ca' Time: a i V,S PM
Received by: i • Date: /rr l/43 `( d Time: jc" '(jr5�� PM
Relinquished by: / t► '` Date: l i ( 3`J f Time: '� S AM PM
Received by: Date: Time: J AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX 71 Meritech Pick-up ['Delivered ❑ Other
**Samples shipped on Friday must be FedEx and must be dearly labeled for Saturday delivery,NO SIGNATURE REQUIRED'*
SAMPLE RECEIVING (Laboratory Use Only)
linquished by: •
Received by: Date: j, (� d Time: is ts- AM i6
Sample Temperatures(°C): j- (4 / ` Co ! I Sample Condition:
11111411110-- WHITE=Laboratory copy YELLOW=Client copy -4111111111111111=111

MERITECH, INC. t ((1\a'
Meritech Sample ID#: 4 1 1 t
BjQassay Samp/e Chainof Custody
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: TcWk- d - Ptak-- UL ? PO#:
Contact Person: , S}..�(4{,�bsv� NPDES#: NCW 445-1fe
Address: ,S'4ftt i4L(t 3 ue R Z Phone:
City: }iYiut.,( Pipe#: WI
County: L X< State: IVL Zip: 7-15 .t,
SAMPLE INFORMATION
Sample Site: TG
Sample Type: ❑Grab T Composite #of containers: 2-
Sampling Time: Start Date: Start Time: 0100 A{il PM
End Date: IL-IS- r }r End Time: ( dJ AO PM
***Triple rinse sample container with sample before filling.Completely fill the sample container with no air space.
Pack the sample cooler► completely in ice. The sample must be<6.0°C upon receipt at the laboratory'
c
Collector'sName: Print: l.:S f-IK� Signature:
TOXICITY TEST INFORMATION
Test Required: 71 Chronic(7 days) Test Organism: ❑Ceriodaphnia dubia (water flea)
❑Acute(24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: % Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION r
Relinquished by: ( 1 C ..4'� Date: if-Ir-4' Time: �c2 y J. AM
Received by: Date: 1/7/5/18 Time: /0 / 5 AM 400
Relinquished by: `--" Date: ///f S/,8 Time: /6 yQ AM.111.
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:[] UPS ❑ Fed EX 111 Meritech Pick-up ❑Delivered ❑ Other
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED**
SAMPLE RECEIVING (Laboratory Use Only)
inquished by:
Received by: Date: I t I t 5 l6 Time: ((.0 t U AM 10
Sample Temperatures(°C): I i -1 / (` iI / I Sample Condition: �T
WHITE=Laboratory copy YELLOW=Client copy -411111111111111

MERITECH, INC. Meritech Sample ID#:11 l Qtp f
Bioassay Sample Chain of Custody
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
p • CLIENT INFORMATION
a,,�, QI-
Client: 1oc f--c/1Ju-%.r (.t.t,j--7 PO#:
Contact Person: t 'Sti0 k..w,>S^ NPDES#: NC 0044&c
Address: ,S-fJ't'L I4•it-Ive
Phone:
City:
Pipe it:
County: k .t State: IJ L Zip: 2 7 5 7-4
SAMPLE INFORMATION
Sample Site: (L (,./w'V F
Sample Type: ❑Grab Eil Composite it of containers: 3
Sampling Time: Start Date: (1 (S-c' Start Time: 6r(:uJ Ali PM
End Date: ((-Its,-c 6 End Time: Oi;u J AO PM
'*'Triple rinse sample container with sample before filling.Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be<6.0°C upon recei at the laborato
Collector's Name: Print: a,r,i 1--c-1o,(A. Signature:
TOXICITY TEST INFORMATION
Test Required: it Chronic(7 days) Test Organism: ❑Ceriodaphnia dubia (water flea)
❑Acute(24-48 hours) [7r Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: L V % Test Concentrations(if multiple dilutions):
Comments:
i � SHIPPING INFORMATION
lN�' Date: j{- 14-c Time: {/� AM P ,
Relinquished by. ��
Received by: /41-; Date: -(L� Time: t D ' ( . AM jjRelinquished • , Date: /Hie
-(' -(,- Time: (D.!. AM:Y�Received b Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX Meritech Pick-up ❑Delivered ❑ Other
"Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED**
SAMPLE RECEIVING(Laboratory Use Only)
linquished by: ° i
l/ 1Received by: Date: C l !l(,1,t�7 Time: (NO AM -tSample Temperatures(°C): I lk / jc / 1( `r-) i Sample Condition: 7609
1111111111111010- WHITE=Laboratory copy YELLOW=Client copy -44111111111

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Shapiro - Wilk' s Test for Normality
D = 0 . 0502
W = 0 . 9616
Critical W = 0 . 8840 (alpha = 0 . 01 , N = 24)
W = 0 . 9160 (alpha = 0 . 05 , N = 24)
Data PASS normality test (alpha = 0 . 01) . Continue analysis .

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Bartlett ' s Test for Homogeneity of Variance
Calculated B1 statistic = 7 . 5823 (p-value = 0 . 1808)
Data PASS B1 homogeneity test at 0 . 01 level . Continue analysis.
Critical B = 15 . 0863 (alpha = 0. 01, df = 5)
11 . 0705 (alpha = 0. 05, df = 5)

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
ANOVA Table
SOURCE DF SS MS F
Between 5 0 . 0204 0 .0041 1.4623
Within (Error) 18 0 . 0502 0 . 0028
Total 23 0. 0706
(p-value = 0 .2507)
Critical F = 4 .2479 (alpha = 0 . 01, df = 5, 18)
= 2 .7729 (alpha = 0 . 05, df = 5, 18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0 . 05)

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Dunnett ' s Test - TABLE 1 OF 2 Ho:Control<Treatment
TRANSFORMED MEAN CALCULATED IN SIG
GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0 . 05
1 Control 0 . 7793 0 . 7793
2 22 . 5 0 . 7345 0 . 7345 1 . 1986
3 45 0 . 6915 0 . 6915 2 .3502
4 75 0 . 7248 0 . 7248 1 .4597
5 90 0 . 7123 0 . 7123 1 . 7945
6 100 0 . 6968 0 . 6968 2 .2096
Dunnett critical value = 2 .4100 (1 Tailed, alpha = 0 . 05, df = 5, 18)
Title : FVTC
File : FVTCGrowth Transform: NO TRANSFORMATION
Dunnett ' s Test - TABLE 2 OF 2 Ho:Control<Treatment
NUM OF MIN SIG DIFF % OF DIFFERENCE
GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL
Control 4
22 . 5 4 0. 0900 11 .5 0 . 0448
3 45 4 0. 0900 11 .5 0 . 0878
4 75 4 0. 0900 11 .5 0 . 0545
5 90 4 0 . 0900 11 .5 0 . 0670
6 100 4 0 . 0900 11 . 5 0 . 0825

Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date:12/14/2018
Facility: Fuquay Varina NPDES#NC00 66516 Pipe#: County: Wake
L/aliora, ry: Meritech, Inc. Comments)
Signature of Operator in Res onsible Char e
9 P py 9 2
�t
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Water Sciences Section
Aquatic Toxicology Branch
Division of Water Resources
1621 Mail Service Center
Raleigh,N.C.27699-1621
Test Initiation Date/Time 12/4/2018 4:36 PM Avg Wt/Surv. Control 0.614 Test Organisms
%Eff. Repl. 1 2 3 4 r' Cultured In-House
Control Surviving# 10 10 10 10 %Survival 100.0 C_ Outside Supplier
Original# 10 10 10 10
Wt/original(mg) 0.628 0.652 0.644 0.530 Avg Wt(mg) 0.614 Hatch Date: 12/3/18
22.5 Surviving# 10 10 10 10 %Survival 100,0 Hatch Time: 3:00 pm CT
Original# 10 10 10 10
Wt/original(mg) 0.675 0.554 0.499 0.576 Avg Wt(mg) 0.576
45 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.591 0.705 0.699 0.563 Avg Wt(mg) 0.640
75_1 Surviving# 10 10 9 10 %Survival 97.5
Original# 10 10 10 10
Wt/original(mg) 0.613 0.594 0.611 0.551 Avg Wt(mg) 0.592
90 Surviving# 10 10 10 9 %Survival 97.5
Original# 10 10 10 10
Wt/original(mg) 0.687 0.620 0.695 0.648 Avg Wt(mg) 0.663
100 Surviving# 10 10 10 9 %Survival 97.5
Original# 10 10 10 10
Wt/original(mg) 0.600 0.581 0.620 0.574 Avg Wt(mg) 0.594
Water Quality Data Day
Control 0 1 2 3 4 5 6
pH(SU) Init/Fin 8.09 / 7.92 8.20 / 7.93 8.21 / 8.16 8.27 / 7.83 8.21 / 7.72 8.20 17.87 8.07 i 7.81
DO(mg/L) Init/Fin 7.71 1 7.09 7.66 / 7.27 7.69 / 6.96 7.68 / 6.88 7.68 17.47 7.85 / 6.48 7.52 / 6.99
Temp(C)'nit/Fin 24,8 124,5 24.8 / 24.6 24.4 / 25.2 24.7 / 24.5 25.4 / 24.9 25.2 / 24.9 24.9 / 24.1
High Concentration 0 1 2 3 4 5 6
pH(SU) Init/Fin 7.19 / 7.80 7,50 / 7.86 7.00 / 7.43 7.16 / 7.36 6.95 17.16 8.22 / 7.02 6.74 17.12
DO(mg/L) Init/Fin 8.35 17.04 7.59 / 7.19 8.39 17.11 7.72 / 7.09 8.26 / 7.42 7.85 / 6.41 8.32 16.83
Temp(C)IniUFin 25.1 / 25.6 25.2 / 25.4 24.9 / 25.9 25.3 / 24.3 25.0 124.6 24.7 / 25.0 25.7 125.3
Sample 1 2 3 Survival Growth Overall Result
Collection Start Date 12/3/2018 12/5/2018 12/6/2018 Normal fl r) ChV >100
Grab Horn.Var. 171 Fj
Composite(Duration) 24.0 24.0 24.0 NOEC 100 100
Hardness(mg/L) 48 58 60 LOEC >100 >100
Alkalinity(mg/L) 56 27 14 ChV >100 >100
Conductivity(umhos/cm) 422 469 478 Method Steel's Dunnett's
Chlorine(mg/L) <0,1 <0.1 <0.1
p.at Receipt(°C) 1.3 1.8 1.8 Stats Survival Growth
Conc. Critical Calculated Critical Calculated
Dilution H2O Batch# 1337 1338 1339 1340 1341 22.5 10 18 2.41 1.0135
Hardness(mglL) 42 44 44 42 44 45 10 18 2.41 -0.7027
Alkalinity(mglL) 55 57 58 59 51 75 10 16 2.41 0.5743
Conductivity(umhos/cm) 199 219 233 213 209 90 10 16 2.41 -1.3243
100 10 16 2.41 0.5338

Meritech, Inc. (Lab # 027)
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Client: FVTC #of Organisms per Chamber. 10 Start Date: 12/4/18 Time: 'mil`36/7/1
NPDES#: 0066516 Test Vessel Size: 400 ml End Date: 12/11/18 Time: I - ? t.
Initiated by: (,r)/L(_./ Test Solution Volume: 250 ml
Date/Time Fed: 12/4/18 1:7OOr'.. Temp. of Stock: (4(7" °C Randomization: No
Date/Time Born: 12/3/18 3:00 PM cr #of Reps: 4 Incubator#: I
Organism Source: Aquatox, Inc. Test Organism: Pimephales promelas Analyst(s): MR, CD, LV, SB
Transferred by: Fed by:
Date Time Initials Date Time 1 Initials Time 2 Initials
Day 1 12/5 it: ri L J/ j7 Day 0 12/4 l- 39)1,1 6_1/
12 6 1 vt 4/1 /t�.-" Da 1 12/5 • (4 4✓1 ' d- 30
Day2 .�.! ( Y
Y
/ � A
Day3 12/7 7( 3 i L.2/(7 Day2 12/6 0 0/441
Day4 12/8 7:t(f):Frt r.✓/7/} Day3 12/7 7 ..• i i J
Day 5 12/9 I-) }—pr^ / Day4 12/8 � �/� ( J � O )J>p It-P---
Day 6 12/10 fa 2(,4vl .Nlc�j�/,�/ Day 5 12/9 q`.il.4N- /&y 3/ /Op71jU`7i �1
Ark
Day6 12/10 i
Test Termination Data:
Initials/Signature: L-1/ ‘4"..dr/ArAll 6-0/61
End Date: 12/11/18 �/v

MER./TECH, 1I11C,.
ENVIRONMENTAL LABORATORIES
t WIF A Olvlslon of Water Technology and Controls, Inc.
Chemical and Physical Determinations
Client: FVTC NPDES#: 0066516 Start Date: 12/4/18
End Date: 12/11/18
Day
Concentration: control 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
Soft Synthetic Feshwater [� /F`
Batch#:/337 /33- / 33Lr? (CJ /3"// --�
pH: Initial X( q3,n ,/ ,,�t! Vi c ft?i 'PG 6-9-
Final 9 - g, ) (0 .17K 7• 70-
D.O.: Initial •--i-q-( 727/_0(0 -7i ( 5
Final r-f: Z� l.t/1Cj(Y 0.8 (0.(1,Y
7
Temp.: Initial 1,1Cf, ' ')t' . 021,7 r i \ 2
Final a.t'( � ,��c 2 ..i 7 y -/ 9 I I
Conductivity: Initial / A D ac- a l ) (<2
Final / C '2c)-S a 3 1 2 T7 )-its icpI
Residual Chlorine: <-01 <0 4011 <rn 1 fie, Cc,.
Hardness: Ct 1-1LJ L Li y u --�
Alkalinity: 5143 -3- 5y ' ) G9
Day
Concentration: 22.50% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial ., f- -3 /3 7.7 3 '8"1-1 27- v21)
Final `:71 irt-{' 767. 7iyu`i •
D.O.. Initial / 2 F. 0 1 t.09
Final 7j( "7, 03 lP r 1 LQ j 7.11 (Q<
Temp.: Initial C(.7 7. c) ac ,
Final 2.5, (1 "2I c az��� act, �.`��? �� r 0 cc!'
Conductivity: Initial 24-7 �--v ac7 ''t-1 7-7 1 )106(
Final O 2'EC7
Residual Chlorine: Cia .oy I Ge, I Zc),l <0, ( Cw I EcI, 1
Day
Concentration: 45.0% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial // 57 7 75 27 l-7 s ?1.194 7, 149
Final �7 7, L 77 7-(o 77 tp 10 7 9
D.O.: Initial .116 gtOt� �, �7 74..z. fir
Final toil, C��� (Orc5 /Wt99 / �� . 67,i�� b3
Temp.: Initial i2i. U _24, 6 ) 0-�,0 _ •0.
Final ?\-(•1 fd-t-tc(2 at1,5 ` ,
Conductivity: Initial Qq 02 0� 3R,1 1(o -4'3 0
Final 3• 1 L "J O
Residual Chlorine: .e <011 I Co I ern( EU I ‹-cn 1

M 7TECH, /N(C_
ENVIRONMENTAL LABORATORIES
- A !'>:VA/on e l t-V Mr>i-ti-It/rbr/aryY'�^rl Gran n'1S.,//rc.
Chemical and Physical Determinations
Page 2 of 2
Client: FVTC NPDES#: 0066516 Start Date: 12/4/18
End Date: 12/11/18
Day
Concentration: 75% 0-1-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial )/1 s-to 7, 30 3s 4 S), ( 777 �a
Final 7-0-q -7,Y6 Y65 7,a9 310 256i
D.O.: Initial 7 ( (pc �C�� �7 Sg �c/�- 7� S
Final (0,�j 1 7 2 1 (01C(b> (M9Sa %
Temp.: Initial ,5"• ) ��. -2 1,-7 olu S 7
Final 2L( Q -L-7 ,Qtf, 7 � a Y,� ?- , t)
Conductivity: Initial 22 3`, y D$' Li
Final 378 �j � v( 1, Cf 1 y 9-Lib O Lit I) 3(06
Residual Chlorine: <o ( ) (6) <oi i cLA1
Day
Concentration: 90% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial 7 Q.1 . S� 7-it, r) 77 0- 2.�b (0,19
Final . .J 7,J5 id `7) 19 7f'?U 1 1�J 3,3c9
D.O.: Initial .07 �� /c2-1 -3 i3 8, 1 U 7- 7 i n661
Final (0,9q .7•� •' I ' no (7,47 (o=c ?
Temp.: Initial 05.j e? g�� �( y ' r . 3n, "7df- a.3•
Final o'Zj. a�fj �j �S. t ' i -1 9c fcl
Conductivity: Initial tiu j _! 3? L..J y q , 4 `1
Final )( L p55 L4 Y5 c 5t Li 7 g LVi4
Residual Chlorine: .Cc.-ht ��,� <ca( Lv,( Lci I Ccn' �a
Day
Concentration: 100% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial 7,f q -zoo 7�i f( (Ro s �' IQ/ aFinal ti0 7 ?}Lis' 7t ( 7. l )(LaiD.O.: Initial '7 9 'i3`7 ,�' �a, 1 ,4/.5-9-A,
�,�,` C,�
Final 7i4 1 / 1 1 O t 7. COqCo, i 1'3
Temp.: Initial .a, `zvt l #2,5 age° ?y 7
Final D-S. (, 5. aS. " 2'-v `)-Y. ?fr.o P-6 3
Conductivity: Initial (-MCI Lidi LI 6 7 7 % 7 g �� 1
Final -! ,-1 i-{ ut5 - S.Z. F / /t--3 Hip 51
tif
Residual Chlorine: Lov l L ( Lcr I Gci, ( LEn 1 col <6,1
Hardness:
Alkalinity: '

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 12/4/18
End Date: 12/11/18
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
0 � ) V v I
Rep AV -- --
0
VRep B 0 _____L 0
Rep 0 C 0 C 0 -C—fa-- .-122--
—Lz_
�
G
Rep D 0 0 0 0 _12__ ._4._ ____61_.
centration 22.50%
ThursdayFridaySaturdaySundayMondayTuesday
Wednesday
Tuesday Wedn y
Rep A 0 --0-- 6 0 ,, __79-.
Rep B 0 __0_ 0 0 O U ______a
RepC 0 —0_ 0 _ _ 0 -- 0
Rep D 0 0 __ _ U 6 0 0 D
V
Concentration 45.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 —41— U
CoJ
Rep B 0 ___ _ 71
© o ____ .-
Rep C 0 0 0 0 6 —0-- l
Rep D 0 —�—
0 0 6 Cl t

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 12/4/18
End Date: 12/11/18
Concentration 75%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
RepA 0 _ / (e)
1 1) 0 d ��
,...../
Rep B 0 _ 0 f 1 0 0 0 _—b-
1
Rep C 0 0 C o -rJ_. 0
\L .� a.—RepD 0 u 0 0
rit-
0-4046
;oncentration 90%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
RepA 0 f`/ / 74 9 --0-_
r_rj___Rep B 0 _ _____t;_)___ v —C)_— 0
Rep C 0 ___ __ _ 0 _ ---)--
Rep D 0 () ! _ a__ 0 v 0
Concentration 100%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
RepA 0 ____ _ /1 6 C)
b. --a—
Rep B 0 p CL U — 0 0 _
Rep C 0 —a_ _ " ___ v (7 V ()
Re0 0 `� v
p D 0 l b

il/IERI TECH
ENVIRONMENTAL LABORATORIES
‘tgileA OfVlsion of Water Technology and Co nlrnls,Mc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC Page: 1 of 2
NPDES #: 0066516 Outfall:
Date of Test: 12/4/18
Initial weights taken on: 11 / by: CV Organism: Pimephales promelas
Final weights taken on: 1 f 2'f 3-1 ; by: bt-,1
PAN WT. PAN + ORG. WT.OF ORG. MEAN
0
CONC REP (mg) WT. (mg) (mg) #ORG. WT./ORG. (mg) SURV /o
A g� ,6o g30, F 6, re 1e (,(00 too
B A7 5 6ti gt9I I6 6,5 1() . r)-65Q ion
//l� 6 LH I //
D U. J / F 351 qq 51 30 /0 06 36 iC'
C l7 0 0.6,76 /on
22.50% B Ea(, q 7 L/13 5( 5L/ /() Q,55 L/ too
cI (-q LJ I 0 (-01 /(;)9
D PoNigi 35!I( 5 7 JO (),57 /)O
A WZ.6E S39 5c 59/ /0 <) ,5q/ /Do
0 B /I� / � Z JO O ' ��� 10
45.0%
1 5� � 0
c 8/ gg &I 7 6t q? 1() O,c qc Lao
D gg.-77o g33.33 5. 63 /0 6(663 /co

1,
MERI TECH /NC
ENVIRONMENTAL LABORATORIES.
A DR Isivn of Wdter T+zchnolovy and Confm/s,fnc_
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC Page: 2 of 2
NPDES #: 0066516 Outfall:
Date of Test: 1214/18
Initial weights taken on: l/- lR by: (V Organism: Pimephales promelas
Final weights taken on: / -13-r by: (,c/
PAN WT. PAN+ ORG. WT.OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) #ORG. WT./ORG. (mg) SURV%
A W1 .93 0(1,36) 613 /010 ()%/3 /�o
75% B '12. -33 ?/1 a 7 l 1 C 5( / c)
c w55.47 t, 7 61) 1 l 0,6I1 4C�
D g.253. 77 E r1 -V, 53/ )() 0, 1 l04
A g2s3.1-a g39 2 t 6! R7 in os6F7 Ioo
90% B R "_1,661 Fari Qô l« 0 'o /oo
gog(3 ?,35,5' c, q5" l0 c.),6 q6 /o0
D3�o '361 (1c1 Er a(01-/
A EJ 7/ R ��3�� . lOO k7 «. ( OO /(30
100% B WE. 95 R3gf .70. 5, Fi )o of 5'T loo
c 7t 7, 5a R9337, 6126 )6) 0,G"2o /00
D �a6i. p0 311 q 57/ 0, 57L/ ()

. MERITECH, INC. Merltech Sample ID#: 1 W,1%10I
Bioassay Sample Chain of Custody
... .le 642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike.reedanmeritech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: Ttx41„A. o- Fu1t1ci PO#:
Contact Person: 16444,4,.. S4.714c,,.sa,,� NPDES#: NCtSO&L.05-ip
Address: S'la 4,t 1 toe I2 . Phone:
City: Rtq.h._./ Pipe#:
County: ( .K.-a- State: e, Zip: 2:15-Z Lc
SAMPLE INFORMATION
Sample Site: 1 eft4r.- C1' 't r)T P
Sample Type: ❑Grab 11 Composite #of containers: Z.
Sampling Time: Start Date: 1 Z-3-1`6 Start Time: 01‘...c.PJ AO PM
End Date: 12--4-II( End Time: a=0.3 Ain PM
**'Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be<6.0°C upon receipt at the laboratory""'*
Collectors Name: Print: er 'SRi
1.t1A Signature: et
TOXICITY TEST INFORMATION
1st Required: VI Chronic(7 days) Test Organism: VI Ceriodaphnia dubia (water flea)
❑Acute(24-48 hours) GA Pimephales promelas (fathead minnow)
❑ Mysldopsis bahia (shrimp)
%
IWC: ��6 Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION �/
Relinquished by: CIS./. S f:t-tvA.n Date: 1Z-t{-I V Time: /0 .10 S- AM PM
Received by: . Date: .L4.t Time: 10 ,1 ��,� PM
Relinquished by: Date: (r2 0'-( Time: G.--�t[' AM t
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Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:0 UPS ❑ Fed EX 1 Meritech Pick-up ❑Delivered ❑ Other
"Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED**
SAMPLE RECEIVING(Laboratory Use Only)
,,alinquished by:
Received by: Date: /li/Lf h Time:'(/iU AM V
Sample Temperatures(°C): ( -3 ! r ' 3 i / Sample Condition: C C--4(
4/3
ANAMMII 1A/L11TC-,1 ..knr..+nrtr rnnv VP! I t1W=("Mani ennv -4111111110

MERITECH, INC. -Merltech Sample ID#: 1,04 (1(t3
Bioassay Sample Chain of Custody
642 Tamco Rd,Reidsville NC 27320 .
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reedt meritech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: dvh Olt C avow— U4 rli+ot PO#:
•
Contact Person: Aciebti j{'t.p Ln,.,san NPDES#: NC OO 46 5 i C.
Address: Phone:
City: Pipe#:
•
County: State: Zip:
SAMPLE INFORMATION
'- .(-c Sample Site: (erllLte C�re,k . Ian 4-
Sample Type: ❑Grab 0 Composite #of containers: -•
Sampling Time: Start Date: i Z` ^113 Start Time: 0 170 AM PM.
End Date: j 2'G--13 End Time: 0906 AM PM.
***Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be<6.0°C upon receipt at the laboratory***
K' /-3
Collectors Name: Print: �t�oy 4�, Signature: a ✓1
TOXICITY TEST INFORMATION
est Required: Chronic(7 days) Test Organism: VI Cerlodaphnla dubla (water flea)
❑ Acute(24-48 hours) vi Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: 90 % Test Concentrations(if multiple dilutions):
Comments:
�� ` SHIPPING INFORMATION
Relinquished by: --<---
, ►__ ____ Date: (2—6`1 V Time: /a. 5 AM diglP
Received b : //� Date: /.2/6 /I 8 Time: l a/ - - AM dip
Relinquished b /G_ Date: J 2/0 /I/$ Time: /to ?--0 AM OP
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Sample Temperature(°C): •
Method of Shipment:❑ UPS ❑ Fed EX yA Meritech Pick-up ❑Delivered ❑ Other
**Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED"*
MPLE RECEIVING(Laboratory Use Only)
A
,..- /<-131:
..eiinqulshed by: :„.....„
Received by: &)'''.5r'--------
Date: ` (,.el C Time: 7&:,,?ti AM el
Sample Temperatures(°C): t-5 i I` V i I Sample Condition: .. `-"2
WI.IITF=I ahnratnry rnnv YELLOW=Client coov -41111=1111111•11111101

MERITECH, INC. Meritech Sample ID#: 12/[JTI a �� `
Bioassay Sample Chain of Custody
- 642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client:�p✓n �t- F✓Q .1Q —�16-'11 PO#:
Contact Person: 4jet f"l 4I—<Phvel;Cori NPDES#: NC OO66S1 6
Address: Phone:
City: Pipe#: 1.
County: State: • Zip:
SAMPLE INFORMATION
Sample Site: t,!"to Cf c E
Sample Type: ❑Grab 0 Composite #of containers: 3
Sampling Time: Start Date: 12"6-1 g' Start Time: 1 I•00 AM PM
End Date: I 2—7-1 a End Time: 11 100 AM PM
**'Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be<6.0°C upon receipt at the laboratory*
1 fir-.
Collector's Name: Print: kb'j _V n 5 Signature: I,
TOXICITY TEST INFORMATION
Test Required: VI Chronic(7 days) Test Organism: Cerlodaphnla dubia (water flea)
❑Acute(24-48 hours) 4 Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
{WC: 90 % Test Concentrations(if multiple dilutions):
Comments:
,.� SHIPPING INFORMATION
Relinquished by: /;Yt9 Date: I Z' 7 -/ Time: /•R 5 0 AM
Received by: Date: /L//7 //g Time: /02_5-0 AM irar
Relinquished by: /��— Date: /c- 7//R Time: / ' "eD AM
Received by: Date:' Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:[] UPS ❑ Fed EX Meritech Pick-up ❑Delivered ❑ Other
'*Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED**
/SAMPLE RECEIVING (Laboratory Use Only)
linqutshed by: rr��
Received by: i
Date: Z(4-((k- Time: 15 LEA AM PfW
/j J
Sample Temperatures(°C): Ct L` I i A I Sample Condition:
111111=1111.► WHITE=Laboratory copy YELLOW= Client copy

Title: FVTC
F FVTCGrowth Transform: NO TRANSFORMATION
Shapiro - Wilk' s Test for Normality
D = 0 . 0493
W = 0 .9755
Critical W = 0 .8840 (alpha = 0 .01 , N = 24)
W = 0 . 9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0 .01) . Continue analysis.

Title: FVTC
F - : FVTCGrowth Transform: NO TRANSFORMATION
Bartlett ' s Test for Homogeneity of Variance
Calculated Bi statistic = 6 .2510 (p-value = 0.2826)
Data PASS B1 homogeneity test at 0. 01 level . Continue analysis .
Critical B = 15 . 0863 (alpha = 0 .01, df = 5)
11. 0705 (alpha = 0 . 05, df = 5)

Title: FVTC
F. - : FVTCGrowth Transform: NO TRANSFORMATION
ANOVA Table
SOURCE DF SS MS F
Between 5
0. 0213 0 . 0043 1. 5552
Within (Error) 18 0 .0493 0 .0027
Total 23 0 . 0706
(p-value = 0 .2229)
Critical F = 4 .2479 (alpha = 0 . 01, df = 5, 18)
2 .7729 (alpha = 0. 05, df = 5, 18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0 .05)

Title: FVTC
F. - p: FVTCGrowth Transform: NO TRANSFORMATION
Dunnett's Test - TABLE 1 OF 2 Ho:Control<Treatment
TRANSFORMED MEAN CALCULATED IN SIG
GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0 . 05
1 Control 0 . 6135 0 . 6135
2 22 . 5 0 . 5760 0 .5760 1. 0135
3 45 0 . 6395 0 . 6395 -0 . 7027
4 75 0 . 5923 0 . 5923 0 .5743
5 90 0 .6625 0 .6625 -1. 3243
6 100 0. 5938 0 . 5938 0 . 5338
Dunnett critical value = 2 .4100 (1 Tailed, alpha = 0 . 05, df = 5, 18)
Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Dunnett 's Test - TABLE 2 OF 2 Ho:Control<Treatment
NUM OF MIN SIG DIFF % OF DIFFERENCE
GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL
Control 4
z 22 . 5 4 0 .0892 14 . 5 0 . 0375
3 45 4 0 . 0892 14 . 5 -0. 0260
4 75 4 0 . 0892 14 . 5 0 .0212
5 90 4 0. 0892 14 .5 -0 . 0490
6 100 4 0. 0892 14 .5 0 .0198

Title: FVTC
F FVTCSurvival Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
D = 0. 0225
W = 0. 6683
Critical W = 0 . 8840 (alpha = 0. 01 , N = 24)
W = 0 . 9160 (alpha = 0. 05 , N = 24)
Data FAIL normality test (alpha = 0 . 01) . Try another transformation.
Warning - The first three homogeneity tests are sensitive to non-normality
and should not be performed with this data as is.

Title : FVTC
F" . FVTCSurvival Transform: NO TRANSFORMATION
Hartley' s Test for Homogeneity of Variance
Bartlett ' s Test for Homogeneity of Variance
These two tests can not be performed because at least one group has
zero variance .
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless .

Title: FVTC
F41 : FVTCSurvival Transform: NO TRANSFORMATION
Steel ' s Many-One Rank Test - Ho: Control<Treatment
MEAN IN RANK CRIT. SIG
GROUP IDENTIFICATION ORIGINAL UNITS SUM VALUE DF 0. 05
1 Control 1 . 0000
2 22 . 5 1 . 0000 18 .00 10 . 00 4 . 00
3 45 1 . 0000 18 . 00 10 . 00 4 . 00
4 75 0 . 9750 16 .00 10 . 00 4 . 00
5 90 0 . 9750 16 . 00 10 . 00 4 . 00
6 100 0 . 9750 16 . 00 10 . 00 4 . 00
Critical values are 1 tailed ( k = 5 )

I
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date:1/17/2019
Facility: Fuquay Varina NPDES#NC00 66516 Pipe It: County: Wake
'Laboreritech Inc. Comments
� - ; '
Signature of Operator In Re poQibie Charge
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Water Sciences Section
1 Aquatic Toxicology Branch
Division of Water Resources
1621 I11ai1 Service Center
Raleigh,N.C.27699-1621
Test Initiation Date/Time 1/8/2019 4:33 PM Avg WUSurv. Control 0.724 Test Organisms
Eff. Repl. 1 2 3 4 r Cultured in-House
Control Surviving# 10 9 9 10 %Survival 95.0 17 Outside Supplier
Original# 10 10 10 10
Wt/original(mg) 0.683 0.748 0.587 0.728 Avg Wt(mg) 0.687 Hatch Date: 1/7/19
22.5 Surviving# 9 9 10 10 %Survival 95.0 Hatch Time: 3:00 pm CT
Original# 10 10 10 10
Wt/original(mg) 0.776 0.711 0.683 0.658 Avg Wt(mg) 0.707
%
45 Surviving# 10 10 8 10 Survival 95.0
Original# 10 10 10 10
Wt/original(mg) 0.614 0,794 0.533 0.658 Avg Wt(mg) 0.650
75 Surviving# 10 9 10 10 %Survival 97.5
Original# 10 10 10 10
Wt/original(mg) 0.790 0.638 0.616 0.684 Avg Wt(mg) 0.682
90 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.775 0,565 0.679 0.664 Avg Wt(mg) 0.671
100 Surviving# 10 10 10 9 %Survival 97.5
Original# 10 10 10 10
Wt/original(mg) 0.785 0.585 0.709 0.657 Avg Wt(mg) 0.684
Water Quality Data Day
Control 0 1 2 3 4 5 6
pH(SU)Init/Fin 8.01 17.85 7.98 / 7,81 8.23 / 8.12 8.26 18.08 8.28 17.98 8.22 / 7.99 8.24 17.71
DO(mg/L) Ina/Fin 7.71 1 6.95 7.29 / 7.04 7,87 / 7.26 7.76 / 7.74 8.15 / 7.53 8.15 / 7.41 7.89 / 6.56
Temp(C)Init/Fin 24.5 / 25.2 24.6 1 24.9 24.6 / 24.0 25.2 / 24.3 24.5 / 24.7 24.4 124,1 24.6 / 24.2
High Concentration 0 1 2 3 4 5 6
pH(SU)Init/Fin 7.41 / 7.97 7.54 / 8.03 7.44 / 7.99 7.80 / 8.14 7.89 / 7.92 7.65 / 7.92 7.70 / 7.73
DO(mg/L) Init/Fin 8.26 / 6.84 7.53 17.25 8.15 / 7.10 7.68 / 7.74 8.11 17.25 8.45 / 7.28 8.20 / 6.32
Temp(C) Init/Fin 25.1 125.8 24.3 / 25.0 25.7 / 25.2 24.6 124.7 25.9 124.9 25.3 / 24.1 25.2 / 24.5
Sample 1 2 3 Survival Growth Overall Result
Collection Start Date 1/7/2019 1/9/2019 1/10/2019 Normal r) PI ChV >100
Grab Horn.Var. ri PI
Composite(Duration) 24.0 24.0 24.0 NOEC 100 100
Hardness(mg/L) 46 48 46 LOEC >100 >100
Alkalinity(mg/L) 72 80 75 ChV >100 >100
Conductivity(umhos/cm) 465 488 490 Method Steel's Dunnett's
Chlorine(mg/L) <0.1 <0.1 <0.1
p.at Receipt(CC) 1.8 1.8 1.9 Stats Survival Growth
Conc. Critical Calculated Critical Calculated
Dilution H2O Batch# 1348 1349 1350 1351 22.5 10 18 2.41 -0.3544
Hardness(mg/L) 44 44 44 46 45 10 19 2.41 0.6354
Alkalinity(mg/L) 50 55 52 54 75 10 20 2.41 0.0778
Conductivity(umhos/cm) 193 208 236 217 90 10 22 2.41 0.2723
100 10 20 2.41 0.0432

Meritech, Inc. (Lab # 027)
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Client: FVTC #of Organisms per Chamber. 10 Start Date: 1/8/19 Time: . 33pi
n.
NPDES#: 0066516 Test Vessel Size: 400 ml End Date: 1/15/19 Time: / (`.5 0 -.-
initiated by: l Test Solution Volume: 250 ml
Date/Time Fed: 1/8/19 -,I:_CL' )'Nlv` Temp. of Stock: ''�l!!` •`5 Randomization: le) No°C p
Date/Time Born: 1/7/19 3:00 PM cr #of Reps: 4 Incubator#:
Organism Source: Aquatox,Inc. Test Organism: Pimephales promelas Analyst(s): MR, CD, LV, SB
Transferred by: Fed by:
Date Time Initials Date Time 1 Initials Time 2 Initials
Day 1 1/9 ) i- ,''"t (-0 / 2 Day 0 1/8 .Z:() al , ;' fig „is,
L / 1/9 7 r 30
Day 1 ✓f 1 (.c./ I'5�>n�
Day 2 1/10 � l���� v�l��L�/
/( L.J//C P Day 2 1/10 f li�V �� (�//7:/`Itr�F'-�^ 1`' --
Day 3 1/11 1�1r�"( /�i� r
Day4 1/12 < 0�4iv � 7-
Day3 1/11 (7 (�✓ /30,,,,,,1 (4�
Days 1/13 n 7/0-__ Day 4 1/12 _7;,t )i 4J/ .,)-:3?471„ "4
1/13 (;
?o ,, _ 3:9-0n LO
7• �r
Day6 1/14 - � � ��.
Day 51--- ---�
Day 6 1/14 f-f cj,,�ll b i d tS8
Test Termination Data:
Initials/Signature: j / �/ 7��
End Date: 1/15/19

ENVIRONMENTAL LABORATORIES
'� A olvlslon Of Water technology and Controls, Inc.
Chemical and Physical Determinations
Client: FVTC PIPDES#: 0066516 Start Date: 1/8/19
End Date: 1/15/19
Day
Concentration: control 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
Soft Synthetic Feshwater f y 2/j 1_ ,r� q p
Batch#: l7 ( ^ 7 /G �� / ��U lJ f 4..
pH: Initial ‘,67, 7�tL�g .A ,v CY ?,
Final '/f/ � 2- )70 7 l 1 %Cf 7,71
D.O.: Initial jj'7.7/ -399 ?-i }7-- L . 1 Tl 7c/
Final l o,CIS `u -�� ?"1) 0 7 l 7 97.3 co
Temp.: Initial 2 y, 5 c:2•A•to ,Cc 2- ,<i 21,1 <),I( ,(,
Final �� �� 941 a q, t✓'•:,41-11- 3 4 (
Conductivity: Initial I CO !q t 2 a 1.3 , ,3z.o a /5''1-
Final ;� f( 1 19 i �`�► � V 0(0
Residual Chlorine: �w `c ZoL - 1
Hardness: LA,k4 __1) ) �f 4(0
Alkalinity: r�t j 514 —�
Day
Concentration: 22.50% 70-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial x p �Q 2 OO t8,Oj
�%1
Final �1/,, 9, 676q, 7-q `/u7 7 /Q,
D,O.: Initial c1,\-1 �_D ' / 7 79O
Final (QAb Pr 5? SIU -7 ) b 6,37
Temp.: Initial odtf. 5i , �i-\ L Z5-� , �J 0 , ,lam 2.if,5
Final "af S �.�•!� u(r (,, }`1- 1,1
. 2`l
Conductivity: Initial 256 Y � :�O 47G $$' a (0
Final j p. ,,q90 3 i y 9 2 y-
Residual Chlorine: LrA I ,I „k . l 7.:*())/
Day
Concentration: 45.0% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
-� l
pH: Initial `� ? y 71�1 i 1 ci 1 ` 7,?' 3, -
n
Final 79( 418 ?) ,U O' 0 5 `7, 67
D.O.: Initial `"(F(')/ u U „-- • - ,c2 y
Final 6, j - 1 1)„ �X 7-3ci --Nip 6. 0
Temp.: Initial �i 5,() 2�, - a °2 j;L , 5-`� 7 y,7 .14, 0
Final (�7 .6 �11/07\ 7 t • _ rJ.
Conductivity: Initial 5C q 5by 3� J F 5
Final 510 3( 4 I 1 67 5 .Z 3y
Residual Chlorine: C.0,1 Ce.),, %, 1 ex.,4,C Cc_r,( _ tuft Cu►

M.ER/TECH„ /-/VC_
ENVIRONMENTAL LACCORATOAI ES
A C+iv/�lc.r raY.Waf.', T>c/ri.uiu DY inner t ri rrt+ls. /h�.
Chemical and Physical Determinations
Page 2 of 2
Client: FVTC NPDES#: 0066516 Start Date: 1/8/19
End Date: 1/15/19
Day
Concentration: 75% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial 7z77 � {S�S ���' �h� 'i '7, 7 /
�� �r
Final "7, C, G, pot lit$ 7, g 77/(f) 7,66
D.O.: Initial ,/3 •9 R- -M 3 `�)C��- ?� � P-
Final jt-)1j2 (Q S l —`h'tf/q 7/ / -?)2I(
�`
Temp.: Initial ��, C oZ�j t0 �7'�1 a5`� � �� f,( '�� `i
Final 21 ) q.,f? ptt, /12-J. 0 au,
Conductivity: Initial gi iQ OM 9?it Li ..." 5 !)3
Final r3 ?)Cq Li` 6 Ul i o - tat-it
Residual Chlorine: 4oi(. 1 Ccr► Cc( emu' j a
t
Day
Concentration: 90% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial -7146 `fl I AL R ° 5,04 7 7O
Final °IS aC.t) ?i(771' t -Lc/' 15
D.O.: Initial � A,LA gul m p
Final LA '- 7-3 7 3: 2 (n.
Temp.: Initial 2, tf, 4' 21 ' 7 Rai. i' m, I 2S,
Final ff �� 9- .tit,(-) l ,1 , g` �.Lt. '7 o 7-
Conductivity: Initial .,,q7 y3( `I S l`I q ) -I'��Q.') - 5�/ t`i`>
Final tiL`1 "l� "I�p , �''�� �1 g LA -,
Residual Chlorine: ,c-n.t e"ca < <cA/ ZCL t e U (
Day
Concentration: 100% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial 1,. 7-0_4 41 z b 5
Final - C( k 03 71 1 2 g a 7 /3
D.O.: Initial _' � {r �1i �� t 'tll _pi �vl l� U,
Final Its /� Q : '7 ��� � 6.3Q
Temp.: Initial ��.� 2`� o% 1 �` �, �J i• �l a4.
Final 3 9S"_ g11.ci , 1 "),Li,( 2 .S
Conductivity: Initial Lj(,)v) k 1 L- x �t C�3" L- H
Final - 2'7 11 ! 415 `J Ct& L7 yco 61
Residual Chlorine: Z', e ! ,.(:), eo,
Hardness: t-{ LI'k LE
Alkalinity: '-J� 70 7

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 1/8/19
End Date: 1/15/19
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 _6.3... 9 / ) 7 ) o (� 0
Rep B 0 ---0— ° () v `-' ___0_ l
Rep C 0 it 0 l 0 0 6
ty
RepD 0 (90 0 C 0
^-'ncentration 22.50%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
RepA 0 U 0 ___0_ 0 ��Rep B 0 i 1 _ V 1 b
RepC 0 0 7 0 l v A 0
RepD 0 --a— 0 () 0 v b O
Concentration 45.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
RepA 0 _ Ub 0,
0cc) 0RepB 0 ( 0 0 0 0
Rep C 0 _ a ;f) [] 0 __
RepD 0 0 _ 0 0 _a_ 0

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 1/8/19
End Date: 1/15/19
Concentration 75%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 ;9 tJ / / -0- 9 _ 6
Rep B 0 ,(' 0 0 to
( 0
Rep C 0 0 0 CI 0 9,Thi
Rep D 0 i--} U () L
v
concentration 90%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 0 D __.. 0 0 _ 0
RepB 0 0 0 0 ) 0 0
Rep C 0 (`) 0 0 t/ 0 0 0
C> G /0 --0- ) "___�_.7 0)Rep D 0 _
Concentration 100%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Re /� (/ /
p A 0
v C
Rep 8 0 _6)___ l l 0 0 e7 6
0
Rep C 09 1) 0 0 0 0 CY
0 1
Rep D 0 _____I___ 0 .__ D 0

ENVIRONMENTAL LABORATORIES
: - A fJiYfsiOA of WaterTechroIogynnd Con[r?%s./nc_
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC Page: 1 of 2
NPDES #: 0066516 Outfall:
Date of Test: 1/8/19
Initial weights taken on: / 2- ,7-( 7 by: (A/ Organism: Pimephales promelas
Final weights taken on: /'I6 y( f by: C
PAN WT. PAN + ORG. WT. OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) #ORG. WT./ORG. (mg) SURV%
A Rl qr 6.4) Y�6,LW 6-X.3 Co 04?3 Or)
B R,Q.N F/qI 2 7,-/E q-1 01717 qo
c Ei c, -L/ n Q63/ -5,8 7 0,677 io
D Fc3: 76 31. oil 7 ` �c�' to (.),7Z loo
A •a 3 i. 6 I �s�'3ql 37 7, 76 0 -777 qo
22.50% - B Fo5. (6, o. 9 7 7. /1 1 01711 qo
c 6'i,?-3 o,132 -7 / 10 6?3 /00
D g31, 0 . W3761 6, 5 ? /u 0r65? Ion
A E23,51 FQ,q, 65 6, /
lo no" /00
45.00/0 B a3r o,J n 300 q '7 L( to O,-7qL( id()
c j3, 57 / 7o 5 33 F 0 S33
D O (9 ?1 l6 U 33,5 6tx !O O,6�P 496

MER/TECH.
- ENVIRONMENTAL:LABORATORIES'
_ 'A gfirfsidd pf.Water'-Technology end Commis;jnc..,
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC Page: 2 of 2
NPDES #: 0066516 Outfall:
Date of Test: 1/8/19
Initial weights taken on: / -,,7-/ by: (A/ Organism: Pimephales promelas
Final weights taken on: /-"lG""/ ( by: to
PAN WT. PAN+ ORG. WT.OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) #ORG. WT./ORG. (mg) SURV%
A g32.5, /o, /2 7 q0 l 0.74O /at)
B am. 66 w,34 �" 13 O 3F qO
75% g � �c �?7 (l O 4• E76/5„-� ' 6 /67 Jo c/6/6 1(9()
D 3Lll .3 � Z/ /0 0t t1 /0(9
A ► 7 Wf3/I 7l 75 10 6. 7 -75 / o
90% B x�l6i, qa F 5,55 5 io o3 6 /ov
c m. 61 Plato 61761 to c i 6-7q (a--)
D I71 6/, 03 6t6L/ 10 O, 661--( too
A E17, 56 eZLf j 7FL 10 oi7J kr)
g00% B Rigx2 . az./107 51Kr_') 10 /o0
c R.2.11 8 gay. q7 10( /v 0 70q ict
D 3,)g ?7 F34i 6 6t57 of6s1 q-0

MERITECH, INC. (Lab #027) Meritech Sample ID#: O 1(A 6 n
Bioassay Sample Chain of Custody
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritechlabs.com
CLIENT INFORMATION
Client: -T n oc F4'1 Vel PO#:
Contact Person: $"k .5f4p1,444,so... NPDES#: NC do&6-6T(t
Address: 5'iv5r HT-It the Q2 Phone:
City: Fu4041.1
Pipe#:
County: (..)4V-4-- State: Nt Zip: z1S Z 4
SAMPLE INFORMATION
Sample Site: Terribic erne*((.. W6s)1" P
Sample Type: ❑Grab 14 Composite #of containers: Z
Sampling Time: Start Date: t '-7"l 1 Start Time: U`t;UJ CIA PM
End Date: t-cer—I End Time: Ut Ali PM
***Triple rinse sample container with sample before filling completely with NO AIR SPACE.Pack the sample cooler completely with ice. The
sample must be<0.6°C upone receipt at Meritech*** --
O.Ca y r L vn Signature: //��[�
Collector's Name: Print: T `��,
TOXICITY TEST INFORMATION
;t Required: Chronic(7 days) Test Organism: EA Cerlodaphnia dubia (water flea) -
❑ Acute(24-48 hours) lip Pimephales promelas (fathead minnow)
(] ❑ Mysidopsis bahia (mysid shrimp)
IWC: -(u % ***Friday Samples for Chronic Fathead test must be collected after 9:00 a.m.on Friday***
Comments:
/�6� SHIPPING INFORMATION
Relinquished by: Date: I-Sr--t Time: "Z� e2:•,AM i
Date: i Time: /Z/, AM PM
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CIO
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Date: Time: AM PM
Sample Temperature(°C):
Method ofShipment:❑ UPS ❑ Fed EX Meritech Pick-up Delivered ❑ Other
**Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED**
SAMPLE RECEIVING(Laboratory Use Only)
r
r e1Inquished by: COeceived by:
Date: 1 5 Time: /�I;.,CJ AM 6
Sample Temperatures(°C): e 1 (- ) i / Sample Condition: '7-`L67/
Mrurrc -I ahnro4nn“ren„ YELLOW=Client cony

MERITECH, INC. (Lab #027) Meritech Sample ID tt: C\,v }`kittt
Bioassay Sample Chain of Custody
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reedameritechlabs.com Web Site: www.meritechlabs.com
CLIENT INFORMATION
Client: TOW.A- 0� �Urlt,rr-/ PO#:
Contact Person: sfl(1 G a)u✓L NPDES#: NC00(4.5
-14
Address: Y/317- t-Ltl.fop Phone: .
City: {- Uq,( Pipe#:
County: U4,14'L State: NC.- Zip: Z?Sztt
SAMPLE INFORMATION
Sample Site: TrATito(t. Cv *°C
Sample Type: ❑Grab Composite #of containers:
Sampling Time: Start Date: I-4-1 Y Start Time: Vi!v ) AI PM
End Date: I--to"t 1 End Time: Ui'µ� All PM
***Triple rinse sample container with sample before filling completely with NO AIR SPACE.Pack the sample cooler completely with ice. The
sample must be<0.6°C upone receipt at Meritech***
Collector's Name: Print: (',ti-sS f'�`-/vth Signature: t;/k, �
TOXICITY TEST INFORMATION
st Required: I Chronic(7 days) Test Organism: 01 Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) [ Pimephales promelas (fathead minnow)
GG�� ❑ Mysidopsis bahia (mysid shrimp)
IWC: 1C7 % ***Friday Samples for Chronic Fathead test must be collected after 9:00 a.m.on Friday***
Comments:
SHIPPING INFORMATION p�
Relinquished by: airs k`(•+.v� Date: to-t/( Time: //02 4 PM
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Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑Delivered El Other
**Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED**
SAMPLE RECEIVING (Laboratory Use Only)
....inquished by:
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Sample Temperatures(°C): r 6 i I '' / I Sample Condition: 6-19
I nkr`ro#nrw rnnto VFI I tlW=(lion+rnmi

MERITECH, INC. (Lab #027) Meritech Sample IDtl:O1 i I Ci
Bioassay Sample Chain of Custody
A
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritechlabs.com
CLIENT INFORMATION
Client: I or
/ PO#:
ST'Contact Person: A-CIA k... epr`-j h
eSeti NPDES#: NCO0005`/L
Address:
t I. Phone:
City: F�l ti t^a VA,yr P2. Pipe#: V°
County: wti State: A/ - Zip: .2 75.2-G
j�
SAMPLE INFORMATION
Sample Site: TG (AS)TP
Sample Type: ❑Grab Q'Composite #of containers: 3
Sampling Time: Start Date: 11 '—/d 1 f Start Time: AU t is- AM PM
!End Date: `11— '9 End Time: /0!/5 de, PM
***Triple rinse sample container with sample before filling completely with NO AIR SPACE.Pack the sample cooler completely with ice. The
� sample must be<0.6°C upone receipt at Meritech***
-
Collector's Name: Print: i7cT1/ ,r ftc, Signature: ---1:g1n5
�� TOXICITY TEST INFORMATION
st Required: l�Chronic(7 days) Test Organism: Q C riodaphnia dubia (water flea)
❑Acute(24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis battle (mysid shrimp)
IWC: ?D % ***Friday Samples for Chronic Fathead test must be collected after 9:00 a.m.on Friday***
Comments:
SHIPPING q INFORMATION
Relinquished by: !� r'�le/% Date: I ri /� Time: /-342° AM a
Received b : ----- .' Date: / /I ( /1 I Time: /3a2.O AM P„
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Sample Temperature(°C):
Method of Shipment:❑ UPS 0 Fed EX [Meritech Pick-up ❑Delivered ❑ Other
**Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED**
SAMPLE RECEIVING (Laboratory Use Only)
linquished by: 22---"\,7
Received by: (C)
Date: i 1�,/(7'1 Time: 15..S AM ek
Sample Temperatures CC): ( C' I I Ci I I. Cl I Sample Condition: r C
iwmiiiillkl■..- UnJITG_ I ahnrm+nnu rnrw VFI I t1W=Pliant r•.nnv

Title: fuquay
fuquay Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
D = 0. 1204
W = 0 . 9659
Critical W = 0 . 8840 (alpha = 0. 01 , N = 24)
W = 0 . 9160 (alpha = 0 . 05 , N = 24)
Data PASS normality test (alpha = 0 . 01) . Continue analysis.

Title: fuquay
F11 : fuquay Transform: NO TRANSFORMATION
Bartlett ' s Test for Homogeneity of Variance
Calculated B1 statistic = 1. 5589 (p-value = 0 . 9062)
Data PASS Bl homogeneity test at 0 . 01 level . Continue analysis .
Critical B = 15 . 0863 (alpha = 0 . 01, df = 5)
= 11. 0705 (alpha = 0 . 05, df = 5)

Title: fuquay
Fi1P: fuquay Transform: NO TRANSFORMATION
ANOVA Table
SOURCE DF SS MS F
Between 5 0 .0072 0 . 0014 0 . 2142
Within (Error) 18 0 . 1204 0 . 0067
Total 23 0. 1276
(p-value = 0. 9519)
Critical F = 4 .2479 (alpha = 0 . 01, df = 5, 18)
= 2 .7729 (alpha = 0 .05, df = 5, 18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0 . 05)

Title: fuquay
File: fuquay Transform: NO TRANSFORMATION
Dunnett ' s Test - TABLE 1 OF 2 Ho:Control<Treatment
TRANSFORMED MEAN CALCULATED IN SIG
GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0 . 05
1 control 0 . 6865 0 .6865
2 22 .5 0 . 7070 0 .7070 -0 .3544
3 45 0 . 6498 0 .6498 0 .6354
4 75 0 . 6820 0 .6820 0 . 0778
5 90 0 . 6708 0 . 6708 0 .2723
6 100 0 . 6840 0. 6840 0 . 0432
Dunnett critical value = 2 . 4100 (1 Tailed, alpha = 0 . 05, df = 5, 18)
i
Title: fuquay
File: fuquay Transform: NO TRANSFORMATION
Dunnett ' s Test - TABLE 2 OF 2 Ho:Control<Treatment
NUM OF MIN SIG DIFF % OF DIFFERENCE
GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL
-1_ control 4
22 . 5 4 0. 1394 20 . 3 -0 . 0205
3 45 4 0 . 1394 20 .3 0 . 0367
4 75 4 0 . 1394 20 . 3 0 . 0045
5 90 4 0 . 1394 20 . 3 0 . 0157
6 100 4 0 .1394 20. 3 0 . 0025

r
Title: fuquay - survival
File: fuquaysury Transform: NO TRANSFORMATION
Shapiro - Wilk' s Test for Normality
D = 0 .0650
W = 0 . 8435
Critical W = 0 . 8840 (alpha = 0 . 01 , N = 24)
W = 0 . 9160 (alpha = 0 .05 , N = 24)
1
Data FAIL normality test (alpha = 0. 01) . Try another transformation.
Warning - The first three homogeneity tests are sensitive to non-normality
and should not be performed with this data as is .

Title: fuquay - survival
File: fuquaysury Transform: NO TRANSFORMATION
Hu—ley's Test for Homogeneity of Variance
Bartlett 's Test for Homogeneity of Variance
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless .

Title: fuquay - survival
File: fuquaysury Transform: NO TRANSFORMATION
Steel ' s Many-One Rank Test - Ho: Control<Treatment
MEAN IN RANK CRIT. SIG
GROUP IDENTIFICATION ORIGINAL UNITS SUM VALUE DF 0 . 05
1 control 0 . 9500
2 22 . 5 0 . 9500 18. 00 10 . 00 4 . 00
3 45 0 . 9500 19 . 00 10. 00 4 .00
4 75 0 . 9750 20 . 00 10 . 00 4 .00
5 90 1 . 0000 22 . 00 10 .00 4 . 00
6 100 0 . 9750 20 . 00 10 . 00 4 . 00
Critical values are 1 tailed ( k = 5 )
1