NC0089737_Comments_20180807

McGill
A S S O C I A T F S
RECEBVED/DENRIDWR
AUG 092018
AugustWater Resources
7, 2018
Permitting Section
Ms. Brianna Young
Environmental Senior Specialist
Department of Environmental Quality
Division of Water Resources
Compliance and Expedited Permitting Unit
1617 Mail Service Center
Raleigh,North Carolina 27699-1617
RE: Water Treatment Plant Upgrade
Town of Franklin
NPDES Application Permit#NC0089737
Macon County
Dear Ms.Young:
I am providing and enclosing the following additional information as requested per your
letter dated June 8, 2018 for the NPDES permit application for the Town of Franklin Water
Treatment Plant,NPDES Permit NC0089737:
Item#2:
I am enclosing the test results for aquatic toxicity using Ceriodaphnia dubia at 90%effluent
the wastewater discharge from the WTP. A series of three test were run. Tests on July 12, 2018,
July 19, 2108 and July 26, 2018. Copies of these results are attached.
55 Broad Street P.O. Box 2259 ph: 828.252.0575
Asheville,North Carolina 28801 Asheville,North Carolina 28802 f: 828.252.2518 +++ . mcgillengineers Loin

If there are any questions please give me a call at 828-252-0575 or email at
[e-mail].
Sincerely,
McGILL ASSOCIATES, P.A.
KEITH WEBB, P.E.
Vice President-Principal
Attachments
CC; Summer Woodard—Franklin
Nathanael Moore—Franklin w/attachment
Rob Hartsell—Franklin
J.Meliski-McGill Associates w/attachment
15.00359/Franklin WTP/NPDES/by07july18-npdes response

PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
July 27, 2018
Mr. Joseph Meliski
McGill &Associates
55 Broad Street
Asheville,NC 28801
RE: ETS PROJECT NUMBER: 13431
Dear Mr. Meliski:
Enclosed are toxicity test results for samples from the Franklin WTP received by
Environmental Testing Solutions, Inc. July 10 through July 12, 2018.
Parameter Test Procedure EPA Method Final
Code Number Result
North Carolina Ceriodaphnia Chronic Effluent
TGP3B Toxicity Procedure EPA-821-R-02-013 FAIL
(Ceriodaphnia Pass/Fail Toxicity Test)
If this test was performed as an NPDES requirement or by Administrative Letter, please enter an
F on the Effluent Discharge Monitoring Form (MR-1) for the collection date July 10,2018 using
the parameter code TGP3B.
Additionally, please sign and submit a copy of the DWQ Aquatic Toxicity Form (AT-3)by
August 31, 2018.
If you have any questions concerning these results, please feel free to contact me.
Sincerely,
Jim umner
Laboratory Director
This report should not be reproduced,except in its entirety,without the written consent of Environmental Testing Solutions,Inc.
The results in this report relate only to the samples submitted for analysis.
North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001

• PO Box 7565
• Asheville,NC 28802
•
• Phone: [phone]
Fax: [phone]
-y Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: July 27,2018
Facility: Franklin WTP NPDES#: NC-0089737 Pipe#: 001 County: Macon
Laboratory Performing Test: Environmental Testing Solutions,Inc. Comments:
Signature of Operator in Responsible Charge: .
Signature of Laboratory Supervisor: � Project: 13431
Samples: 180710.34,180712.32
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: _ Start time: End time:
Raleigh,NC 27699-1621 07-11-18 07-18-18 1257 0700
Sample Information Sample 1 Sample 2 Control
Collection start date: 07-10-18 07-12-IS rest Information Start Renewal I Renewal 2 Start Renewal I Renewal
Grab: \ X Treatment: 90% 90% 90% Control Control Control
Composite duration: Initial pH(SU): 7.24 6.58 6.92 7.27 7.25 7.44
Alkalinity(mg/L CaCO3): 30,30,30 Final pH(SU): 7.02 7.08 6.74 7.17 7.32 6.95
Hardness(mg/L CaCO3): 40,40,38 Initial DO(mg/L): 8.1 8.1 7.8 7.9 7.8 7.8
Conductivity(pmhos/cm): 112 75 I,n i,, iio Final DO(mg/L): 8.1 8.0 7.7 _ 8.0 7.8 7.8
Total residual chlorine(mg/L): <0.10 <0.10 Initial Temp.(°C): 24.6 24.9 24.8 24.8 24.7 24.7
Sample Temp.at Receipt(°C): 1.8 1.8 Final Temp.(°C): 24.9 25.1 25.0 25.1 25.1 25.0
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 I I 12 Mean Chronic Test Results
Number of Young Produced 25 26 28 29 28 28. 30 26 27 29 28 27
27.6 Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 5.2
Effluent Percentage 90% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Control: 0 of 11
Number of Young Produced 28 23 19 28 16 0 18 0 0 3 0 10 12.1 IWC: 3 of 12
Adult Survival: (L)ive,(D)ead L L L L L D L D D D D D 56.2 Significant?: No
L %Rea°`lion Final Mortality Significant at: 90%
Effluent Percentage-
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Reproduction Analyses
Number of Young Produced Reproduction LOEC: 90%
Adult Survival: (L)ive,(D)ead I Reproduction NOEC: <90%
%Reduction
Overall Method: Heteroscedastic t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms I '_ 3 4 5 6 7 8 9 10 11 12 Mean Method: Shapiro-Wilk.
Number of Young Produced Statistic: 0.918
Adult Survival: (L)ive,(D)ead Critical Value: 0.884
%Reduction
Equal Variances: No
Effluent Percentage Method: F-Test_
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 60.869
Number of Young Produced Critical Value: 5.320 _
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
%Reduction
Method: .
Effluent Percentage Effluent% Rank Sum Critical Sun,
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean 90%
Number of Young Produced
Adult Survival: (L)ive,(D)ead
%Reduction
Overall Analysis:
Result: FAIL LOEC: 90% NOEC: <90% ChV: <90%
DWQ form AT-3(8/91)Rev. 11/95

Page lof1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0, NC Modification-December 2010,Version 3.0)-Control Bench Sheet
I Control 4: , Date: 07-11-18
Test Grouping Information: Test Organism Information:
Facility Project# Organism Source: In-house Culture 6 _
�'o1�eST L) 1 \ 1 Age: <24-hours old
5 �2A1�Y1-\I.� \"��+�t Source(culture board): 01-a-th B
4 ‘Fela>SPAO— 1'341-k Replicate 4 1 2 3 I 4 1 5 6 7 8 9 111112
13 110\DSO-3 \ � Culture boardcu # 1 (I iI t2J p 3 5 0 3I 11 ►a 21 2�
�� A2� t3�{1la Date and time organisms were (TYI\-tk os—L2— TO 11..,t0
1-Control born between:
Average transfer volume: 0.03 mL
Transfer bowl information: pH(S.U.): -1• fj 3
Daily Renewal Information: Temperature(°c): -1-4•
°%
Day Date
Test Initiation,renewal,feeding or
termination 55W Batch
Selenastrum YWT Location Randomizing
Time Analyst Batch Batch Incubator/Shelt Template
0 07-11-18 Initiation/Feeding
i l'1S1 11 (n-os-IE A Q1-os•1$ 05-31-qs. -2.1).5 GrL2erJ
1 07-12-18 Feeding
0900 7-5
2 07-13-18 Renewal1/Feeding
3 07-14-18 Feeding
nS5S TS
4 07-15-18 Feeding
L.
.os55 T5
5 07-16-18 Renewal2/Feeding x - s
06.50 ak CT\•tt-\!;
6 07-17-18 eedinga(.1R-4R0 -F^
7 07-18-18 Termination �`�
o�ob 1
Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst ^�/ ^✓A. w 1` !-1 S
pH(S.U.) 1.'2'\ t.l� (.2S 32 1.�y �D.gJ�
Dissolved oxygen(mg/L)
_ __ 1.`1 �.b 4 ,B �1 �4 • '1.8
Conductivity(pathos/cm) \So 1._ t
Control,SSW _ 7
I *Alkalinity(mg/L CaCO3) 3o _ 30 — -.30
*Hardness(mg/L CaCO3) 0 ' f 0
*Temperature(°C) `,•& ZS•\ lti`�•1 LS- 45
Isk- 1 'IS.0
'Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation,renewal or termination by
the analyst identified in the Daily Renewal Information table. Alkalinity and hardness performed by the analysts indentified on the test bench sheets and transcribed to this bench sheet.
!I Survival and Reproduction Data:
Day Observations Replicate number
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality l
Renewal One (L=Live,D=dead) ` \--- �--
Number of broods present L1 [ I L I [ it__ I� L L-3_... L L•_
5 Number of young produced It 1'1 I.. ILS- I Lt.`LL= t. t' lb IO11L= 3
Renewal Two Adult mortality V_
(L=Live,D=dead)
Number of broods present L( L I LI LL IL'_ Li 11— LL Li
Number of young produced (` . 13 I2. I 1 I,1 )Lf ti 2 I t sIL{
7 Total young produced Z�1S 1-68 Z F{ 30 1-1° 11 7_51 Z1E- 2^7
Final
Final adult mortality 1
(L=Live,D=dead) -^ L._ k__ l___ L L-
X for 3rd Broods X X x >( Ic �/ \/ x v 7(
Control Acceptance Criteria: �►y /` 7\ /` / \ /` 7_ /`
%of Male Adults(e 20%) /. Mean Offspring/Female ^� /-
%Adults having 3rd Broods(_80%) (On (>15 offspring/surviving female) 1',lp
%Mortality(e 20%) %CV(<40%) 5.2.7.
SOP AT12-Exhibit AT12.3,revision 11-01-14

rrna ,r
Page 1of1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0, NC Modification—December 2010,Version 3.0)-Test Bench Sheet
Paired with Control# ‘ Date: (11-I •1g
Client Town of Franklin NPDES# NC0089737
Facility Franklin WTP Outfall 001
0.04
Project# #fin %-�-1-'0, County Macon
Test Concentration(Chronic Limit) 90% Dilution mL mL Total volume
preparation: Sample Dilution water mL
Samples were not aerated or treated unless otherwise noted on this form. Control,dilution water 270 30 300
and test renewal information are included on the Control Bench Sheet indicated above.
Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst us 1A- VA." tel/ 75
pH(S.U.) •ZK `4.02. 1P.S0 TUC/ °Z- 6,7 L{
c�
Test Dissolved oxygen(mg/L) 8-l 6,1 ti,lI aiv0 .Q 7. 7
Concentration Conductivity(µmhos/cm) //3 eta+ sj
*Temperature(°C) IAA. -.0t.S 1'1.1 -LS.\ 1"k. € 1S-n
—
-pH(S.U.) •2� 10• -
Dissolved oxygen(mg/L) g-\ c.0
100% Conductivity(µmhos/cm) 112.
*Total residual chlorine(mg/L) 40.1.0 -0-10
Sample number Sample 1 1 4. GI 10 .v4 Sample 2 1k ffl t1•l-__
'Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation,renewal or termination by
the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine perfonned by the analyst identified on the Total Residual
Chlorine Bench Sheet and transcribed to this bench sheet.
Survival and Reproduction Data (performed at test concentration):
Day Observations Replicate number
1 2 3 4 I 5 6 7 8 9 10_1 ...3._—11 I 12
2 Adult mortality L L \- L L_ L- -1L L
Renewal One (L=Live,D=dead)
Number of broods present 1 7., 11 11 t I t O I 1 _ I L • L
L
5 Number of young produced l b 13 1 l'>_ 0 S 3 I O
Renewal Two Adult mortality 1__. L_ t.
(L=Live,D=dead) 1. k--
I
Number of broods present L [ L 12_.- L� .r I:
Number of young produced
1S Ib s IL l3 1. , // . I
Final Total young produced -2_8 1'S 1 Z& 16 Q 1 Q d 3
Final adult mortality ` D b D
(L=live,D=dead) `" `—' L
Test was initiated using Sample 1. Sample 2 was used for Renewals One(day 2)and Two(day 5). Samples were diluted to the test concentration prior to use with soft synthetic water
and warmed to 25.0±1.0°C in a warm water bath.
Comments:
Test Results and Statistical Analyses:
Test results Statistics
t%Mortality j-t t-Stat or
JV�• Rank Sum 4 Zi
Mean offspring 1-Tailed
per female 12 el Critical 291.0
Reduction PASS or FAIL FA-IL
from control 2 7
SOP AT12-Exhibit AT12.2,revision 11-01-14

} Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test
EPA-821-R-02-013,Method 1000.0-North Carolina Modification
Quality Control
Verification of Data Entry,Calculations,and Statistical Analyses
Client: Franklin WTP
Test dates: July 11-18,2018
Environmental Testing Solutions,Inc. Project number: 13431
Reveiwed by:
Concentration l)a', Survival Average Coefficient of Percent reduction
(%) Number of young produced by replicate number (%) reproduction variation from control (%)
1 2 3 4 5 6 7 8 9 10 I I 12 (offspring/female) CA)
Control 5 11 13 16 15 14 14 13 14 16 14 17 13
7 14 13 12 14 14 14 17 12 11 15 11 14 100 27.6 5.2 Not applicable
Total 25 26 28 29 28 28 30 26 27 29 28 27
)0% 13 13 4 12 3 0 5 0 0 3 0 10
7 15 10 IS 16 13 0 13 0 0 0 0 0 50 12.1 93.2 56.2
Total 28 23 19 28 16 0 18 0 0 3 0 10
Dunnett's MSD value: 8.908 MSD= Minimum Significant Difference
PMSD: 32.3 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10th percentile) =13%.
Upper PMSD bound determined by USEPA(90`h percentile)=47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile,respectively,of PMSD data from EPA's WET
Interlaboratory Variability Study(USEPA,2001a;USEPA,2001b).
USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency,Cincinnati,OH.
Franklinwtp_07-11-18

0
0 F , ,
0
44,
Environmental Testing Solutions,Inc.
Ceriodaphnia Survival and Reproduction Test-7 Day Survival
Start Date: 7/11/2018 Test ID: CdPFCRNC Sample ID: Franklin WTP
End Date: 7/18/2018 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
Comments:
Conc-% 1 2 3 4 5 6 7 8 9 10
D-Control 1.0000 1.0000 1.0000 1.0000 1.0000 1 0000 1.0000 1 .0000 1.0000 1.0000
D-Control 1.0000 1.0000
90 1.0000 1.0000 1.0000 1.0000 1.0000 0.0000 1.0000 0.0000 0.0000 0.0000
90 0.0000 0.0000
Not Fisher's 1-Tailed
Conc-% Mean N-Mean Resp Resp Total N Exact P Critical
D-Control 1.0000 1.0000 0 12 12 12
*90 0.5000 0.5000 6 6 12 12 0.0069 0.0500
Hypothesis Test(1-tail, 0.05) NOEC LOEC ChV TU
Fisher's Exact Test <90 90
Treatments vs D-Control
Franklinwtp_07-11-18

Environmental Testing Solutions,Inc.
Ceriodaphnia Survival and Reproduction Test-Reproduction
Start Date: 7/11/2018 Test ID: CdPFCRNC Sample ID: Franklin WTP
End Date: 7/18/2018 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
Comments:
Conc-% 1 2 3 4 5 6 7 8 9 10
D-Control 25.000 26.000 28.000 29.000 28.000 28.000 30.000 26.000 27.000 29.000
D-Control 28.000 27.000
90 28.000 23.000 19.000 28.000 16.000 0.000 18.000 0.000 0.000 3.000
90 0.000 10.000
Transform: Untransformed 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
D-Control 27.583 1.0000 27.583 25.000 30.000 5.233 12
X90 12.083 0.4381 12.083 0.000 28.000 93.195 12 4.729 2.718 8.908
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.9177r 0.884 0.20999 0.10897
F-Test indicates unequal variances (p = 6.35E-08) 60.8691 5.31963
Hypothesis Test(1-tail, 0.01) MSDu MSDp MSB MSE F-Prob df
Heteroscedastic t Test indicates significant differences 8.90816 0.32295 1441 .5 64.447 1.0E-04 1. 22
Treatments vs D-Control
Franklinwtp 07-11-18

Ceriodaphnia dubia
�� � Chronic Reference Toxicant Control Chart
Environmental Testing Solutions,Inc. Source• In-house Culture
1.10 1 I I I I I I I I I I I I I I I I I I I
- Control Limits (± 2 Standard Deviations _ -- c -
1.08 - . ... . .—......- • _ • • • –
• • • • -
1.06 – –
• • •
V •
crs •
Z 1.04 – ..® . �.ONIUM......®...mlaa", _
J
aA I I I I I I I I I I I I I I I I I I I I
N - I I I I I I I I I I I I 1 I I I I I I I -
L)
A1.15 – ....................................................................>....,........,..................A. _
CD W73 _ arning Limits
rL
1.10 – –
_ r.._ .. .._... .._........–S .�...�.._.._..—S y_ ,.
-
• • • • •• • •
1.05 - —...._,.e.. ........•..o.. ..,. .. ._.•._......��.._..._..._ .>—."-,,, -
1.00 – –
0.05 1 I L__ I I _1—J_LI I I I 1 1 1 I I _L__I
01 A-1 01 Al O0,1 O. ,1 00,1 0611 1117 Oa 11 1. ,1 0.0,1 O_,1 Oy11 091' 0616 0616 031 0315 005 Qy 14i 101$
c 6 OP Oy O6. O6. O (S 09 10 11P 1ti c (S 03° O Oa. Oy O6- 61
Test date
• 7-day IC25=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodaphnia reproduction (calculated using ToxCalc).
--- Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
— Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
> Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
..«m.- — USEPA Warning Limits (mean logarithmic IC25±SAio converted to anti-logarithmic values,
SA to 10th percentile of CVs reported nationally by USEPA)
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i 351 Depot Street
Asheville,NC 28801
! F.
� Phone: [phone]
'' Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Franklin WTP NPDES#: NC 0089737 Pipe#: 001 County: Macon
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 90%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Grab sample: 9 Sample location: 1,04s it?560e0
Date: -7-'0-6 Time: ' 44 Volume collected for testing: l_L
Number of containers filled for testing: /
Method of transport to laboratory: MC ctiu-
Comments:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
Sample collected by:
4C°WL
O16 .0-jp i
Print Signature :late and time
Relinquished by: Received by: A:4'
i7Mlo-i4 /holly
Print Signature Date and time Print Signature Date and time
Relinquished by: Received by:
q /4
Ibtn�
Print Signature Date and time Print Signature Dale and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
00
Print Signature Date and time Print Signature �t [e an
Custody seals intact?: - Sample temperature upon receipt at ETS(°C):
Yes No Not
used k A
Samples received in good condition?: EMI -
Yes No Total residual chlorine upon receipt at ETS: _ 111
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) Present Absent
Tracking number: '11/4
Project number:1 3 I Sample number: 180q10.3t4
Comments:

.,+\, -.7,26
• .
. .. ,
• PT J e'1?= 3/,/ 351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Franklin WTP NPDES#: NC 0089737 Pipe#: 001 County: Macon
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 90%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Grab sample: Sample location: WASTe. LA640?✓
Date: .7 /2- S /71- )2-it Time: � ;/ Volume collected for testing: 1-l_..
p
/ Number of containers filled for testing: /
Method of transport to laboratory: Pn&c G.ni_&.
Comments:
(
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
Sample collected by: ?/1074.17220
,Q-� /./�./�
J0,AEXT r it?/1,"r `°- " 7
-12—J$
Print Signature Date d time
Relinquished by: Received by:
R6Ba 17 11/11375g 4,t, -7;2-.1;
l -I;
/� -5,4 . f RG ci‘i
Print Signature Date and time Print Signature Date and time
Relinquished by: Received by:
11 Isis
Print Signature Date and time Print Signature Date and time
Sample receipt information: (to be completed by ETS personnel) I
Relinquished to ETS by: Received at ETS by:
- 14. 3C vmple", c),_, - IS1.-I
Print Signature Date and time Print Signature Date and linu-
Custody seals intact?: _ Ill Sn Sample temperature upon receipt at ETS(°C): iYes No Not used 1.
Samples received in good condition?: M
Yes No Total residual chlorine upon receipt at ETS: _ ati
(DPD Presence/Absence Indicator,MDL=0.10 mg/L) Present Absent
Tracking number: tICK
Project number: I1141JI Sample number: I 601-12.W.--
Comments:
O1-12. .-Comments:

•
•
PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
July 31, 2018
Mr. Joseph Meliski
McGill &Associates
55 Broad Street
Asheville,NC 28801
RE: ETS PROJECT NUMBER: 13467
Dear Mr. Meliski:
Enclosed are toxicity test results for samples from the Franklin WTP received by
Environmental Testing Solutions, Inc. July 17 through July 19, 2018.
Parameter Test Procedure EPA Method Final
Code Number
Result
North Carolina Ceriodaphnia Chronic Effluent
TGP3B Toxicity Procedure EPA-821-R-02-013 PASS
(Ceriodaphnia Pass/Fail Toxicity Test)
If this test was performed as an NPDES requirement or by Administrative Letter,please enter a
P on the Effluent Discharge Monitoring Form (MR-1) for the collection date July 17,2018 using
the parameter code TGP3B.
Additionally,please sign and submit a copy of the DWQ Aquatic Toxicity Form(AT-3) by
August 31,2018.
If you have any questions concerning these results,please feel free to contact me.
Sincerely,
ch)11
Sumner
Laboratory Director
This report should not be reproduced,except in its entirety,without the written consent of Environmental Testing Solutions,Inc.
The results in this report relate only to the samples submitted for analysis.
North Carolina Certificate Numbers: Biological Analyses: 37,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001

• PO Box 7565
• Asheville,NC 28802
VP
0 Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: July 31,2018
Facility: Franklin WTP NPDES#: NG 0089737 Pipe#: 001 County: Macon
Laboratory Performing Test: Environmental Testing Solutions,Inc. Comments:
Signature of Operator in Responsible Charge: .
Signature of Laboratory Supervisor: _0 ,,,p„y..c..---.- Project: 13467
Samples: 180717.04,180719.04
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 07-18-18 07-25-18 1150 0830
Sample Information Sample 1 Sample 2 Control
Collection start date: 07-17-18 07-19-11 I est Information Stan Rcnenal I Renewal Start Renewal I Renewal
Grab: X X Treatment: 90% 90% 90% Control Control Control
Composite duration: Initial pH(SU): 6.92 6.54 6.56 7.48 7.50 7.07
Alkalinity(mg/L CaCO3): 30 Final pH(SU): 6.70 6.61 6.60 7.46 7.08 7.02
Hardness(mg/L CaCO3): 38 Initial DO(mg/L): 8.0 8.4 8.0 7.8 7.8 7.7
Conductivity(pmhos/cm): t,; 68 is3_Iaa FinalDO(mg/L): 8.5 8.1 8.1 7.8 7.6 7.9
Total residual chlorine(mg/L): <0.10 <0.10 Initial Temp.(°C): 24.9 25.0 24.7 24.6 24.7 24.6
Sample Temp.at Receipt(°C): 0.8 0.3 Final Temp.(°C): 24.8 24.9 24.9 25.1 25.0 24.9
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan Chronic Test Results
Number of Young Produced 34 30 29 32 28 29. 30 29 29 27 30 28 29 6 Final Control Mortality(%). 0.0
Adult Survival: (L)ive,(D)ead L L L L L L L LL L L L %Control with 3rd Broods: 100
Control Reproduction CV: 6.4
Effluent Percentage 90% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Control: 0 of 12
Number of Young Produced 34 35 33 34 35 29 35 35 32 36 35 35 34.0 IWC: 0 of 12
Adult Survival: (L)ive,(D)ead L L L LL L L LL L L L -14.9 Significant?: No
/Redaction Final Mortality Significant at No Conc
Effluent Percentage-
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Reproduction Analyses
Number of Young Produced Reproduction LOEC: >90%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 90%
%Reduction
Overall Method: Homoscedasn:
Effluent Percentage_ Normal Distribution: Se,
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Method: Shapiro-Wilk',
Number of Young Produced _ Statistic: 0.953
Adult Survival: (L)ive,(D)ead Critical Value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 1.028
Number of Young Produced Critical Value: 5.320
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
%Reduction
Method: .
Effluent Percentage Effluent Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 I2 Mean 90% .
Number of Young Produced
Adult Survival: (L)ive,(D)ead
'„Reduction
-
Overall Analysis:
Result: PASS LOEC: >90% NOEC: 90% ChV: >90%
DWQ form AT-3(8/91)Rev. 11/95

I Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0, NC Modification—December 2010,Version 3.0)-Control Bench Sheet
I Control#: , Date: 07-18-18
Test Grouping Information: Test Organism Information:
Facility Project# Organism Source: In-house Culture
I 6 n it'UP Age: <24-hours old
5 • 0.S Source(culture board): v\•tp•\& A
4 V-RA��L(t3 13`-14.'1 Replicate# 1 2 3 4 5 6 7 8 9 10 11 12
3 (AICs N041THFo(M ISA%.-\ Culture board cup# I- 4 q It '`1 iS it. to Zo-12.21 11
2 L 'le_ke_QtkL 03,11„...l Date and time organisms were -\$•ti max% -vv) \\OZ,
1-Control ' ' , born between:
Average transfer volume: 0.03 mL
ITransfer bowl information: pH(S.U.): 1,s4
Temperature(°C): "VA.$
Daily Renewal Information:
Test Initiation,renewal,feeding or
Selenastrum YWT Location Randomizing
Day Date termination SSW Batch
Time Analyst Batch Batch Incubator/Shelf Template
0 07-18-18 Initiation/Feding "I_(-3 6- 0
I 1 07-19-18 Feedin
l.�9/IS Ts
2 07-20-18 Renewals/Feeding
l0". al-tZ►i;
3 07-21-18 Feeddw 3 O -lc)
4 07-22-18Fee3g8L'U TS
5 07-23-18 ,Renewwall/-FFeeeddiing 6k t)1.11 14.-
1
6 07-24-18 Feedin
O 5•OU -"S
7 07-25-18 Termination
6P5-0 IA
I Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial -T,5
rFiinaall
Concentration Analyst -7 X11 (5�� I S .' TS es ter 1 ,J
I
pH(S.U.) 1.—[ D Sof S d'id c�'p 7.05 '4.07 7.0.2
Dissolved oxygen(mg/L) "7.$' 7•? 7.g' �.(..o 4.7 7 ci
Conductivity(µmhos/cm) /53 M 3 //.✓g
Control,SSW *Alkalinity(mg/L CaCO3) 30 \(j1'f1e, �ZY1,B
I *Hardness(mg/LCaCO3) 3e, \�M
*Temperature co /4-14 -15.‘. WA -LS .O 1A-(o by-'t
*Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures perforated at the time of test initiation,renewal or termination by
the analyst identified in the Daily Renewal Information table. Alkalinity and hardness performed by the analysts indentified on the test bench sheets and transcribed to this bench sheet.
Survival and Reproduction Data:
Observations Replicate number
Day 1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality L _ L ` -L. LL- L. 1_ L,
Renewal One (L=Live,D=dead)
' I'
Number of broods present IZ L L. L LI L `,i L f L `,L Li
I5 Number of young produced \\O lb 1,`11 4S t3 4"Z, t`t 'S2' is 1't1is
Renewal Two Adult mortality L k— L L L.
(L=Live,D=dead)
I Number of broods presentLI L t LL L
Number of young produced ( 1S (1 15 (1 Ito (y t1 12- IL (3
7 Total young produced 3,1 -50 .LS 3.Z le -Let 7o z ^15 .1 j� U
l
Final adult mortality L_ k.... L-- L_ ' �
(L=Line,D=dead)
X for 3rd Broods X X• X- )4- ) x )- )L x > > )(
I Control Acceptance Criteria:
of Male Adults(<_20%)
(i). Mean Offspring/Female
Adults having 3rd Broods(. 80%) (00 7 (?15 offspring/surviving female) a
%Mortality(<_20%) 0 %CV(<40%) 0•r17
SOP AT12-Exhibit AT12.3,revision 11-01-14

Page l of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0, NC Modification—December 2010,Version 3.0)-Test Bench Sheet
Paired with Control# t Date: al-a•ta
1 Client Town of Franklin NPDES# NC0089737
Facility Franklin WTP Outfall 001
1 Project# \% . % \ County Macon
Test Concentration(Chronic Limit) 90% Dilution mL mL Total volume
preparation: Sample Dilution water mL
1 Samples were not aerated or treated unless otherwise noted on this form. Control,dilution water 270 30 300
and test renewal information are included on the Control Bench Sheet indicated above.
I Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final . S
Initial+ Final
Concentration Analyst TS .1, T3 Ts H / (InT3
I pH(S.U.) 69,70 6,541 6.6 1 (.SCO Co.Coo
�.9 p p
Test Dissolved oxygen(mg/L)
01,6
�� ?p d>rd4 0.0 0•y 6. 1 83-,. ? I
Concentration Conductivity(µmhos/cm) 73 77 '18
I - *Temperature(°C) _iZy��•�, --t-A. LS•ju Z�\-c1 n-V' -l}�-�
pH(S.U.) 60.� U �i. � 5 _
Dissolved oxygen(mg/L)
I100% Conductivity(µmhos/cm) CO 3 693*Total residual chlorine(mg/L) p• 10 <o- 1 o
Sample number Sample 1 18 6111.64 Sample 2 1i,n la• O`A,
I *Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation,renewal or termination by
the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual
Chlorine Bench Sheet and transcribed to this bench sheet.
ISurvival and Reproduction Data (performed at test concentration):
Day Observations Replicate number 1
1-1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality
I Renewal One (L=Live,D=dead) 1=_L
,
Number of broods present l= I LE tL IE LE L . L LI
5 Number of young produced 11, 1'l IL. 1q 11 15 (t, u0 1s 14 tS 11
I Renewal Two Adult mortality L. l— L_
(L=Live,D=dead)
Number of broods present ._ t_ L LI_ c� LL LL a 11 . Li. (
I Number of young produced O 1 g 1'+ 15 Ig 14 I , ii 1 (1 ii /0 IR
7 Total young produced 34 3S s3 3� Ns zs - s 3S 3—L
Final 3 V, 35 3%
I Final adult mortality . l— k. L l_ L L
(L=Live,D=dead)
Test was initiated using Sample 1. Sample 2 was used for Renewals One(day 2)and Two(day 5). Samples were diluted to the test concentration prior to use with soft synthetic water
and warmed to 25.0±1.0°C in a warm water bath.
IComments:
I Test Results and Statistical Analyses:
Test results Statistics
Mortality t-Stat or _5�'�
r Rank Sum
I Mean offspring 1-Tailed 1lQ
per female 3L-1.0 Critical 2:5016
Reduction . (14A). PASS or FAIL p55
Ifrom control
SOP AT12-Exhibit AT12.2,revision 11-01-14

• Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test
EPA-821-R-02-013,Method 1000.0-North Carolina Modification
Quality Control
Verification of Data Entry,Calculations,and Statistical Analyses
Client: Franklin WTP
Test dates: July 18-25,2018
Environmental Testing Solutions,Inc. Project number: 13467
Reveiwed by: wk.... .
Concentration Dap Survival Average Coefficient of Percent reduction
(%) Number of young produced by replicate number (%) reproduction variation from control (%)
1 2 3 4 5 6 7 8 9 10 11 12 (offspring/female) (%)
Control 5 16 16 14 15 13 12 14 15 12 15 14 15
7 18 14 15 17 15 17 16 14 17 12 16 13 100 29.6 6.4 Not applicable
Total 34 30 29 32 28 29 30 29 29 27 30 28
90% 5 16 17 16 19 17 15 16 16 15 19 l5 17
7 18 18 17 15 18 14 19 19 17 17 20 1s 100 34.0 5.6 -14.9
Total 34 35 33 34 35 29 35 35 32 36 35 35
Dunnett's MSD value: 1.939 MSD= Minimum Significant Difference
PMSD: 6.6 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10'h percentile) =13%.
Upper PMSD bound determined by USEPA(90th percentile)=47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile,respectively,of PMSD data from EPA's WET
Interlaboratory Variability Study(USEPA,2001a;USEPA,2001b).
USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes I and 2-Appendix.EPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency,Cincinnati,OH.
Frank)inwtp_07-11-18

1
Environmental Testing Solutions,Inc.
Ceriodaphnia Survival and Reproduction Test-Reproduction
Start Date: 7/18/2018 Test ID: CdPFCRNC Sample ID: Franklin WTP
End Date: 7/25/2018 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
Comments:
Conc% 1 2 3 4 5 6 7 8 9 10
0-Control 34.000 30.000 29.000 32.000 28.000 29.000 30.000 29.000 29.000 27.000
D-Control 30.000 28.000
90 34.000 35.000 33.000 34.000 35.000 29.000 35.000 35.000 32.000 36.000
90 35.000 35.000
Transform: Untransformed 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
0-Control 29.583 1.0000 29.583 27.000 34.000 6.358 12
90 34.000 1.1493 34.000 29.000 36.000 5.609 12 -5.712 2.508 1.939
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95306 0.884 -0.3395 2.01484
F-Test indicates equal variances (p = 0.96) 1.02784 5.31963
Hypothesis Test(1-tail, 0.01) MSDu MSDp MSB MSE F-Prob df
Homoscedastic t Test indicates no significant differences 1.93946 0.06556 117.042 3.58712 9.6E-06 1, 22
Treatments vs D-Control
Franklinwtp_07-11-18

® 4Wlrim wifirgi our
_ -7.Z 351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Franklin WTP NPDES#: NC 0089737 Pipe#: 001 County: Macon
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 90%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Grab sample: Sample location: CZ%
Date: '7—)7-/1 Time: 33409 Volume collected for testing: tt--L
Number of containers filled for testing:
Method of transport to laboratory: At,(31Q.
Comments:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
Sample collected by:
Rat it 141 a 1-47.
3 3/fin
Print Signature Date and Ernie
Relinquished by: Received by:
RAatii, d,; ti p )-
Print Si Cnature Date and time Print Signature Date and time
Relinquished by: Received by:
fila,��g
i mil/ '~
Print Signature Date and time Print Signature Date and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received
at ETS by:
•
Punt Signature Date and time Print Signature Date and time
Custody seals intact?: M' MI MI Sample temperature upon receipt at ETS(°C):
Yes No Not used
Samples received in good condition?: w _
Yes Total residual chlorine upon receipt at ETS: _ I ✓ I
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) Pfeunt `I" '
Tracking number: A
Project number: 13414—Sample number: le
O
Comments:
1

'• Tc
PI. = 'iI
11
ID 351 Depot Street
• Asheville,NC 28801
# ., Phone: [phone]
talk .
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Franklin WTI) NPDES#: NC 0089737 Pipe#: 001 County: Macon
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 90%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Grab sample: Sample location: 60\
Date: 01'1 V 18 Time: °tn. Volume collected for testing: 1-, L.
Number of containers filled for testing:
Method of transport to laboratory: M c GM—
Comments: i _Comments:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
Sample collected by:
3 ?rIlA Ett- 064444707-'W
1;zzAn
Print Signature Date and time
Relinquished�p� by:
1 ��D�--}} -7-/q-1 -Received by:
r NERT' AWOL Qo(,,J LI l( Htemy mac. L.
Print Signature Date and time Print 'Signature Date and time
Relinquished by: Received by:
•�15,i'6kF to
Print
4J,Signature Date and time Print Signature Date in:I lime
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
�` SS MND - x-19 I F
V.
J
Print Signature Date and time Print Signature Date and
Custody seals intact?: - 11111 [--- / Sample temperature upon receipt at ETS(°C):
Yes No Not used 0 a3
J .C.
Samples received in good condition?: In -
Yes No Total residual chlorine upon receipt at ETS: _ ./
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) Present \b''„t
Tracking number: d l
Project number:(34101--
T Sample number: IS01 9,6 y
Comments:

•4:i,, r 1 1 Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
Environmental Testing Solutions,Inc. Source: In-house Culture
1.10 IIIIIIIIIIIIIIIIIIII
- Control Limits (± 2 Standard Deviations„ �,,,.—•� ———• -
1.08 — ---------- _
• • • • -
• •
1.06 — • • —
�.•.— —.a • • •
- •
U
Z 1.04 — • —
J
b.0 I IIIIIIIII IIIII IIIII
N - I IIIIIIIII 11111 I I I I I -
V_
>. 1.15 — .., ... —
.R5 Warning Limits
r
1.10 — —
• •— • - • __ • •
1.05 — —..—..—•.—••—..,♦,..—..—••-1°..It.--!.--e••—•._.•—..—..—..—..—..—... —
1.00 — —
0.95 I I 1 1 1 1 1 1 I I I 1 1 1 1 ii 1 II
�i 1i �i 1i OA 1i D.L i 061- 00- 10,1 0c 1l 1.L-1 03,11 �i si 001 0co?,005:,00,55 031$031$0%NI,�5 0 10 9,
03. 13 0a� 05e .0 06� 0i 0$� 09N 1,0. .N ), 01� Q 03, 0Q� 0A� 05� .0 01,
Test date
• 7-day IC25=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodaphnia reproduction (calculated using ToxCalc).
— Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
=N .,r-- — Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
.,. —. Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
,--. , USEPA Warning Limits (mean logarithmic IC25±SA.10 converted to anti-logarithmic values,
SA 10= 10th percentile of CVs reported nationally by USEPA)
i
end and
eviewed by
1
(•'Ma\tuner
I

••
PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
August 03, 2018
Mr. Joseph Meliski
McGill &Associates
55 Broad Street
Asheville,NC 28801
RE: ETS PROJECT NUMBER: 13490
Dear Mr. Meliski:
Enclosed are toxicity test results for samples from the Franklin WTP received by
Environmental Testing Solutions, Inc. July 24 through July 26, 2018.
Parameter Test Procedure EPA Method Final
Code Number Result
North Carolina Ceriodaphnia Chronic Effluent
TGP3B Toxicity Procedure EPA-821-R-02-013 PASS
(Ceriodaphnia Pass/Fail Toxicity Test)
If this test was performed as an NPDES requirement or by Administrative Letter,please enter a
P on the Effluent Discharge Monitoring Form (MR-1) for the collection date July 24,2018 using
the parameter code TGP3B.
Additionally, please sign and submit a copy of the DWQ Aquatic Toxicity Form (AT-3) by
August 31,2018.
If you have any questions concerning these results,please feel free to contact me.
Sincerely,
i Sumner
Laboratory Director
This report should not be reproduced,except in its entirety,without the written consent of Environmental Testing Solutions,Inc.
The results in this report relate only to the samples submitted for analysis.
North Carolina Certificate Numbers: Biological Analyses: 37,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001

• AshevillePONC Box
28802 7565
•'
• 5 Phone: [phone]
= ,Fax: [phone]
. Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: August 03.2018
facility: Franklin WTP NPDES#: NC-0089737 Pipe#: 001 County: Macon
Laboratory Performing Test: Environmental Testing Solutions,Inc. Comments:
Signature of Operator in Responsible Charge: _ .
Signature of Laboratory Supervisor: __0w-...___-_ Project: 13490
Samples: 180724.01,180726.01
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 07-25-18 08-01-18 1238 0818 '
Sample Information Sample I Sample 2 Control
,
Collection start date: 07-24-18 07-26-1 Test Information Stan Renewal I Renewal 2 Stan Renewal I Renewal 2
Grab: X \ Treatment: 90% 90% 90% Control Control Control
Composite duration: Initial pH(SU): 6.89 6.69 6.96 7.20 7.17 _ 7.57
Alkalinity(mg/L CaCO3): +I Final pH(SU): 6.70 7.21 6.25 7.04 7.67 6.72
Hardness(mg/L CaCO3): 40 Initial DO(mg/L): 8.0 8.0 8.1 7.9 7.9 7.8
Conductivity(µmhos/cm): S+ Final DO(mg/L): 8.1 8.1 8.1 8.0 8.0 8.2
Total residual chlorine(mg/L): 0.10 Initial Temp.(°C): 24.7 24.7 25.0 24.6 24.6 24.8
Sample Temp.at Receipt(°C): n o Final Temp.(°C): 25.0 24.8 24.9 24.9 25.0 24.7
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of Young Produced 28 24 28 25 26 30 27 29 27 29 26 27 27.2 Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 6.4
Effluent Percentage 90% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Control: 0 of 12
Number of Young Produced 36 33 30 32 35 34 37 30 38 34 34 34 33.9 IWC: 0 of 12
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L -24.8 Significant?: No
Reduction Final Mortality Significant at: No Cone.
Effluent Percentage
Treatment 3 Organisms I 2 3 4 5 6 7 8 9 10 11 12 Mean Reproduction Analyses
Number of Young Produced Reproduction LOEC: >90%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 90%
Reduction Overall Method: Homoscedastic t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Method: Shapiro-Wilk's
Number of Young Produced Statistic: 0.973
Adult Survival: (L)ive,(D)ead Critical Value: 0.884
%Reduction
Equal Variances: Ye.
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 _ 6 7 8 9 10 11 12 Mean Statistic: 1.988 _
Number of Young Produced Critical Value: 5.320
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
%Reduction Method:
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 II 12 Mean 90% .
Number of Young Produced
Adult Survival: (L)ive,(D)ead
%Reduction
Overall Analysis:
Result: PASS LOEC: >90% NOEC: 90% ChV: >90%
DWQ form AT-3(8/91)Rev. 11/95

-w
_a,"' Oxy
111
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia Page 1 of 1
(EPA-821-R-02-013 Method 1002.0,NC Modification—December 2010,Version 3.0)-Control Bench Sheet
IIControl#: I Date: 07-25-18
Test Grouping Information: Test Organism Information:
Facility Project# Organism Source: In-house Culture
II 6 __� Age: <24-hours old
5
____..----1( ....6-1S Source(culture board): in-t'1-t& IN I
4 Replicate# 1 I 2
3 Culture board cup# n lb f 3 it f 4 5 6�S 'S t°1J1728 9 11411112 Y131
Ys
2 F�QPN 1tC�dw3 13�{�� Date and time organisms were 01-15.1 0 V`1 b TT) 1-r11::
L1Q
1-Control born between:
Average transfer volume: 0.03 mL
, g Transfer bowl information: pH(S.U.): '\.S'S
Daily Renewal Information: Temperature(°c): /A..%
Test Initiation,renewal,feeding or
Day Date termination SSW Batch Selenastrum YWT Location Randomizing
Time Analyst Batch Batch Incubator/Shelf Template
0 07-25-18 Initiation/Feeding
kZ3CS. ZS1 , optEla 01-usAI vvcs-‘2 z$\ et,..1K
1 07-26-18 Feeding-,\b0
2 07-27-18 Renewal r1//Feeding
Q'16S cn-1&4k
1 3 07-28-18
4 07-29-18 FeedOnpn,z_..)
Feedi��SQn5 T
5 07-30-18 Renewal 2/Feeding b
0100cn-I�I-1 k
6 07-31-18 Feeding
U Z a
eti
7 08-01-18 iermin 0�1b
1 4
1 Chemical Analyses: J Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst T5 'T'S -1-5 IX, ,A
pH(S.U.) 7.d.0 7.04 7.17 "4=41- -4-.5 - 10.1 2, 1
Dissolved oxygen(mg/L) 7 / Q,o 7.9 E3.0 /}e �
Conductivity(µmhos/cm) / Vv
Control,SSW :�5g 15 7 OyPj 1
*Alkalinity(mg/L CaCO3) /1 it ar,e - , 0 I
I *Hardness(mg/L CaCO3) `t �y't \✓\r
*Temperature(°C) -1.q. 14.4‘ -LA.L "G- U . l. AA 1 x
*Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation,renewal or termination by
Ithe analyst identified in the Daily Renewal Information table. Alkalinity and hardness performed by the analysts indentified on the test bench sheets and transcribed to this bench sheet.
Survival and Reproduction Data:
Day Observations Replicate number
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality
Renewal One (L=Live,0=dead) V._ l..
Number of broods present I - L1 [ L 11, 11- 1 -2.. I L ii. 0_, 1-L. 1 /
I 5 Number of young produced 11- ll, I IS 1-Z. Ib I f 11.„ %1 I S t1„ t q
Renewal Two Adult mortality
(L=Live,D=dead) 1 V I_ L. L 1_ 1_ 1✓ k__,
Number of broods present L< L\ 1± L` t` I1 t I t LL 11 � , ,
Number of young produced lip 1 — I S lI b 14 r-1 t3 I1 IIP 14 i I
7 Total young produced pp 2.4
Final --Ls, Z 4i ZS 2-la 30 1"1 .2_`1Z1 2`t1-43 Z,
Final adult mortality 1 t
it (L=Live,D=dead) L L__. �. ` (`
X for 3rd Broods x, )(. ) x ><- X ( x / x X
`i
Control Acceptance Criteria:
of Male Adults(S 20%)
0). Mean Offspring/Female �}^�
%Adults having 3rd Broods(>_80%) 100- (>_15 offspring/surviving female) 0(-}-2-
%Mortality(<_20%) V /. %CV(<40%) /_,q?
SOP AT12-Exhibit AT12.3,revision 11-01-14

Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
I (EPA-821-R-02-013 Method 1002.0, NC Modification—December 2010,Version 3.0)-Test Bench Sheet
I Paired with Control# I Date: 01-1S•t&
Client Town of Franklin NPDES# NC0089737
Facility Franklin WTP Outfall 001
IProject# 1"Ag0 County Macon
Test Concentration(Chronic Limit) 90% Dilution ml mL Total volume
I preparation: Sample Dilution water mL
Samples were not aerated or treated unless otherwise noted on this form. Control,dilution water 270 30 300
and test renewal information are included on the Control Bench Sheet indicated above.
I Chemical Analyses: ^Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst /J p5p G.70 I 5 T5 �1' �%m1/4-I pH(S.U.) 6,P / (0,07-.2i1 c0.1 1 Z-S-
Test Dissolved oxygen(mg/0g•0 g. I e.o ei,l at ill
Concentration Conductivity(µmhos/cm) 152 lug Loco
I *Temperature(°C) t.{.'\ 'LS•O l'�•'� L� �+-U ZAy
pH(S.U.) . 6.706.56
Dissolved oxygen(mg/L) S 0 g• 1
I 100% Conductivity(µmhos/cm) 54 p5"?'
*Total residual chlorine(mg/L) G ,l0 c 0•1 O
Sample number Sample 1 1&6.12.14.
12`( O 1 Sample 2 ,C crv-Lsco•O
I *Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation,renewal or termination by
the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine perfonned by the analyst identified on the Total Residual
Chlorine Bench Sheet and transcribed to this bench sheet.
Survival and Reproduction Data (performed at test concentration):
I
Observations Replicate number
Day 1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality L L L_- 1. L L V.
I
Renewal One (L=Live,D=dead) II ^, L-1,_. ' ' ' , ',
Number of broods present L= LL.
5 Number of young produced lo 1 ta• t to t' \1 1" 15 ti- 1. Ito IS
I Renewal Two Adult mortality
(L=Live,o=dead) l--
Number of broods present I 1 1 It lk t 11 , I l I L 1 t It [1_
I 7 p
Number of young produced 2_0 t S I I b O CI (& (S 10 t I 1 `
Total young produced
Final 3 1. 33 13 2 3S SA 3l 34 'M "A 3%\ 3SI 1
Final adult mortality L L.- l_. L U. L L
(L=Live,D=dead)
Test was initiated using Sample 1. Sample 2 was used for Renewals One(day 2)and Two(day 5). Samples were diluted to the test concentration prior to use with soft synthetic water
and warmed to 25.0±1.0°C in a warm water bath.
IComments:
L Test Results and Statistical Analyses:
Test results Statistics
%Mortality (� t-Stator
v . Rank Sum --4-.13g
Mean offspring ^ 1-Tailed
per female
`i Critical 6t)
%Reduction11 �1 PASS or FAIL P(�A
ssfrom control •�/
SOP AT12-Exhibit AT12.2,revision 11-01-14

r Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test
EPA-821-R-02-013,Method 1000.0-North Carolina Modification
Quality Control
Verification of Data Entry,Calculations,and Statistical Analyses
Client: Franklin WTP
Test dates: July 25-August 01,2018
Environmental Testing Solutions,Inc. Project number: 13490
Reveiwed by:
Concentration I)a Survival Average Coefficient of Percent reduction
(o�u) Number of young produced by replicate number (%) reproduction variation from control (%)
1 2 3 4 5 6 7 8 9 10 11 12 (offspring/female) (%)
Control - 12 12 13 15 12 16 14 12 11 15 12 14
I i, 12 15 10 14 14 13 17 16 14 14 13 100 27.2 6.4 Not applicable
l'otal 28 24 28 25 26 30 27 29 27 29 26 27
90% 5 16 18 12 16 15 17 19 15 18 16 16 IS
'n I, 18 16 20 17 18 Is 'u I8 18 19 100 33.9 7.3 -24.8
Dotal 36 33 30 32 35 34 37 30 38 34 34 34
Dunnett's NISD value: 2.190 MSD= Minimum Significant Difference
PMSD: 8.1 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10th percentile) =13%.
Upper PMSD bound determined by USEPA(90th percentile)=47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile,respectively,of PMSD data from EPA's WET
Interlaboratory Variability Study(USEPA,2001a;USEPA,2001b).
USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency,Cincinnati,OH.
Franklinwfp_07-25-18

.
fie
Environmental Testing Solutions,Inc.
Ceriodaphnia Survival and Reproduction Test-Reproduction
Start Date: 7/25/2018 Test ID: CdPFCRNC Sample ID: Franklin WTP
End Date: 8/1/2018 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
Comments:
Conc% 1 2 3 4 5 6 7 8 9 10
D-Control 28.000 24.000 28.000 25.000 26.000 30.000 27.000 29.000 27.000 29.000
D-Control 26.000 27.000
90 36.000 33.000 30.000 32.000 35.000 34.000 37.000 30.000 38.000 34.000
90 34.000 34.000
Transform: Untransformed 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
0-Control 27.167 1.0000 27.167 24.000 30.000 6.440 12
90 33.917 1.2485 33.917 30.000 38.000 7.272 12 -7.733 2.508 2.190
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.9726 0.884 -0.1512 -0.2235
F-Test indicates equal variances (p = 0.27) 1.98762 5.31963
Hypothesis Test(1-tail, 0.01) MSDu MSDp MSB MSE F-Prob df
Horn oscedastic t Test indicates no significant differences 2.18957 0.0806 273.375 4.57197 1.0E-07 1,22
Treatments vs D-Control
Frankl inwtp_07-25-18

f. 141 p: u.(° C 351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Franklin WTP NPDES#: NC 0089737 Pipe#: 001 County: Macon
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 90%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Grab sample: Sample location: 00‘
Date: 7-2 -ITime: 1i 56,Am Volume collected for testing: t-1/4-
Number
Number of containers filled for testing:
g
Method of transport to laboratory: } 6-t _
Comments:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
Sample collected by:
rE� q fir` S
Pnnt Signature Date and time
Relinquished by: Received by:
R086.77/I"Arr/AL/5W 7,..,'2irtfi e[C(IP( / 7—
P 7
Print Signature Date and rime Print tiipn;aw. Date and tune
Relinquished by: Received by:
Print Signature Date and tune Print Signature Date and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
—11111Xi TL4teil AG I co
Pnnt Sipn:uw: Date and nme Print Signature Dane and nnie
Custody seals intact?: — „ Sample temperature upon receipt at ETS(°C): //y� _!
Yes Not used V. v
Samples received in good condition?: —
No Total residual chlorine upon receipt at ETS:
1-71
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) Pres"'
Tracking number: N O Q/�
Project number:1344 o Sample number: fVL`I.01
Comments:

" 7 2 —
351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Franklin WTP NPDES#: NC 0089737 Pipe#: 001 County: Macon
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 90%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Grab sample: Sample location: 00%
Date: 7`24'/g Time: 7. 2-S-4/$1 Volume collected for testing: I- L.
Number of containers filled for testing: /
Method of transport to laboratory: Kr 6-ILL
Comments:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
Sample collected by:
1T 1140561 64estatitittall 1-16-1 I
Print Signature Date and ante
Relinquished by: Received by:
36-2T adv t .Mp4sit✓
Print Signature Date and time Print Signature Date antime
Relinquished by: Received by:
-YOAtt NA
Print 5' Signature Date and time Print Signature Date and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
5 b-1-lb•l
Jv►��eati 164S
Print Signature Date and time Pn��, Signature Date and ante
Custody seals intact?: - _ Mg Sample temperature upon receipt at ETS(°C):
Yes No used O.l'C
Samples received in good condition?: -
Total residual chlorine upon receipt at ETS: _
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) Present Absent
Tracking number: ('s
Project number: Sample number: 1'60'7210.0)
Comments:

•
.. " , _fr-Mfty Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
„ Environmental Testing Solutions,Inc. Source: In-house Culture
1.10 111111111111111 I I I I I
- Control Limits (± 2 Standard
Deviations), --
— • -
.a ---
-.. r-• • • •_
1.08 - --- ,�
-
• • • • -
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1.06 - • • -
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V
Z
: :: i
. • -
J
1 I 1 1 I I I 1 I I 1 1 1 I 1 I I Ii I
Ln
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co Warning Limits
n
1.10 - -
-
• • • . • • •
1.05 - _.....-...,_...+,,.-...•..,—..—•...,•••