NC0021253_Renewal Application_20180430

CITY OF HAVELOCK
41111 Post Office Box 368
1 Governmental Ave.
4*-°R .Fuersp•• Havelock,NC 28532
April 27, 2018
Wren Thedford
Water Quality Permitting Section—NPDES RECEIVE��®���pWR
Division of Water Resources APR ® 201B
North Carolina Department of Environmental Quality
1617 Mail Service Center water Kesaurces
Raleigh,NC,27699-1617
Permitting Section
Re: NPDES Permit Renewal Application(NC0021253)
City of Havelock Wastewater Treatment Plant
City of Havelock,North Carolina
Ms. Wren Thedford:
The City of Havelock Public Services Department is permitted to discharge 2.25 million gallons per day(mgd)of
treated effluent from the City of Havelock Wastewater Treatment Plant(WWTP)to the Neuse River under North
Carolina NPDES Permit NC0021253.The application package for the NPDES permit renewal was submitted to the
Division of Water Resources on December 15,2017.The renewal application did not include the four required
Second Species Toxicity tests.In the December 2017 correspondence,the City committed to completing the required
testing by the NPDES permit expiration date of June 30,2018.Attached to this correspondence are three(3)copies of
the following revisions to our NPDES renewal application package,which results in a completed application:
• Revised EPA Form Part E,Toxicity Testing Data
• Attachment(Tab F of the December 2018 renewal application package): Second Species Toxicity Testing
Results 2017-2018
The current permit's Supplement to Permit Cover Sheet includes a lime addition system as part of the last bulleted
item under Section 1.The City of Havelock has not operated the lime system in more than a decade.Class B
pathogen reduction and vector attraction reduction requirements have been met without the use of the lime
stabilization system.The City plans to continue to utilize the current practice of sludge thickening via polymer
addition,gravity belt thickener,and solids holding storage to achieve Class B residual treatment requirements.There
is no future intention or treatment plan that includes the use of lime addition to meet residuals treatment requirements.
The City requests the lime system be removed from the Supplement to Permit Cover Sheet as part of this permit
renewal cycle,which will allow the City to remove the system from the site and repurpose the footprint for non-
process related needs.
If you have any questions regarding any of the NPDES permit renewal application materials, please contact me at
[phone] or awimberlyAhavelocknc.us.
Phone[phone] www.havelocknc.us Fax[phone]

Sincerely,
Ashley Wimberly,
Assistant Public Services Director
cc:
Mary Sadler,PE,Hazen and Sawyer
Todd Davis,PE, Hazen and Sawyer
Ted Elshof, Hazen and Sawyer
RECEIVED/DENR/DWR
APR 3 0 2018
Water Resources
Permitting Section
Phone[phone] www.havelocknc.us Fax[phone]

Hazen aff-u-ifgQ OCA UR A N�C�tlO�t�r'Lad
Hazen and Savwer DATE. JOB NO.
4011 Westchase Blvd,Suite 500,Raleigh,NC 27607 April 27, 2018 30906-006
ATTENTION
NPDES Unit
TO Wren Thedford RE City of Havelock Wastewater Treatment Plant
Division of Water Resources NPDES Permit Renewal Application
Water Quality Permitting Section - NPDES
1617 Mail Service Center NC0021253
Raleigh, NC 27699-1617
WE ARE SENDING YOU . Attached ❑ Under separate cover via the following items:
El Shop drawings 0 Prints ❑ Plans ❑ Samples ❑ Specifications
❑ Copy of letter 0 Change order • Revisions to NPDES Permit Renewal Applications
COPIES DATE NO. DESCRIPTION
1 27-Apr NPDES Permit Application Modification Cover Letter
3 27-Apr EPA Form Part E, Toxitiy Testing Data
3 27-Apr Attachment Tab F: Second Species Toxicity Testing Results 2017-2018
THESE ARE TRANSMITTED as checked below:
For approval ❑ Furnish as submitted ❑ Resubmit copies for approval
❑ For your use ❑ Furnish as corrected ❑ Submit copies for distribution
❑ As requested ❑ Revise and resubmit ❑ Return corrected prints
❑ For review and comment ❑
❑ FOR BIDS DUE 20 0 PRINTS RETURNED AFTER LOAN TO US
REMA12KS
COPY TO •
File(1 copy)
SIGNED: Todd Davis,PE
If enclosures are not as noted,kindly notify us at once.

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
City of Havelock WWTP, NC0021253 Renewal Neuse River
SUPPLEMENTAL APPLICATION INFORMATION
PART E. TOXICITY TESTING DATA
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points: 1)POTWs with a design flow rate greater than or equal to 1.0 mgd;2)POTWs with a pretreatment program(or those that are
required to have one under 40 CFR Part 403);or 3)POTWs required by the permitting authority to submit data for these parameters.
• At a minimum,these results must include quarterly testing for a 12-month period within the past 1 year using multiple species(minimum of two
species),or the results from four tests performed at least annually in the four and one-half years prior to the application,provided the results
show no appreciable toxicity,and testing for acute and/or chronic toxicity,depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition,this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition,submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity,provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation,if one was conducted.
• If you have already submitted any of the information requested in Part E,you need not submit it again. Rather,provide the information
requested in question E.4 for previously submitted information. If EPA methods were not used,report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below,they may be submitted in place of Part E.
If no biomonitoring data is required,do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
complete.
E.1. Required Tests.
Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
®chronic 0 acute Four Fathead Minnow(second species)tests
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one
column per test(where each species constitutes a test). Copy this page if more than three tests are being reported.
Test number: 1 Test number: 2 Test number: 3
a. Test information.
Test Species&test method number Mediia beryllina Mediia beryllina Mediia beryllina
EPA-82-R-02-014:1006 EPA-82-R-02-014:1006 EPA-82-R-02-014:1006
Age at initiation of test 10 days 9 days 10 days
Outfall number 002 002 002
Dates sample collected 11/28/17—12/6/18 12/10/17—12/15/17 1/21/18—1/26/18
Date test started 11/30/2017 12/12/2017 1/23/2018
Duration 7 days 7 days 7 days
b. Give toxicity test methods followed.
Short-term Methods for Estimating the Short-term Methods for Estimating Short-term Methods for Estimating
Manual title Chronic Toxicity of Effluentts and the Chronic Toxicity of Effluentts and the Chronic Toxicity of Effluentts
Receiving Waters to Marine and Receiving Waters to Marine and and Receiving Waters to Marine
Estuarine Organisms Estuarine Organisms and Estuarine Organisms
Edition number and year of publication 3rd Edition,2002 3rd Edition,2002 3rd Edition,2002
•
Page number(s) All All All
c. Give the sample collection method(s)used. For multiple grab samples,indicate the number of grab samples used.
24-Hour composite X X X
Grab
d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection X X X
After dechlorination
EPA Form 3510-2A(Rev. 1-99). Replaces EPA forms 7550-6&7550-22. Page 15 of 20

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
City of Havelock WWTP, NC0021253 Renewal Neuse River
Test number: 1 Test number: 2 Test number: 3
e. Describe the point in the treatment process at which the sample was collected.
Sample was collected: Final Effluent Final Effluent Final Effluent
f. For each test,include whether the test was intended to assess chronic toxicity,acute toxicity,or both
Chronic toxicity X X X
Acute toxicity
g. Provide the type of test performed.
Static
Static-renewal X X X
Flow-through
h. Source of dilution water. If laboratory water,specify type;if receiving water,specify source.
Laboratory water Artificial Salt Water Artificial Salt Water Artificial Salt Water
Receiving water
i. Type of dilution water. If salt water,specify"natural"or type of artificial sea salts or brine used.
Fresh water
Salt water Instant Ocean Sea Salt Instant Ocean Sea Salt Instant Ocean Sea Salt
j. Give the percentage effluent used for all concentrations in the test series.
3.75,7.5, 15, 30,60 3.75,7.5, 15,30, 60 3.75,7.5, 15, 30, 60
k. Parameters measured during the test. (State whether parameter meets test method specifications)
pH X/Yes X/Yes X/Yes
Salinity X/Yes X/Yes X/Yes
Temperature X/Yes X/Yes X/Yes
Ammonia
Dissolved oxygen X/Yes X/Yes X/Yes
I. Test Results.
Acute: N/A
Percent survival in 100% 0/0
effluent %
LC5o
95%C.I. 0/0
Control percent survival 0/0
Other(describe)
EPA Form 3510-2A(Rev. 1-99). Replaces EPA forms 7550-6&7550-22. Page 16 of 20
_ i

r
FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
City of Havelock WWTP, NC0021253 Renewal Neuse River
Chronic:
NOEC 60% 7.5% 60%
IC25 N/A N/A N/A
Control percent survival 97.4% 97.5% 100
Other(describe)ChV >60% 10.6% >60%
(Chronic Value)
m. Quality Control/Quality Assurance.
Is reference toxicant data available? Yes Yes Yes
Was reference toxicant test within Yes Yes Yes
acceptable bounds?
What date was reference toxicant test 11/30/2017 12/12/2017 01/13/2018
run(MM/DD/YYYY)?
Other(describe) N/A N/A N/A N/A
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ® No If yes,describe:
E.4. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information,or information regarding the
cause of toxicity,within the past four and one-half years,provide the dates the information was submitted to the permitting authority and a summary
of the results.N/A
Date submitted: / / (MM/DD/YYYY)
Summary of results: (see instructions)
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 17 of 20

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
City of Havelock WWTP, NC0021253 Renewal Neuse River
SUPPLEMENTAL APPLICATION INFORMATION
PART E. TOXICITY TESTING DATA
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points: 1)POTWs with a design flow rate greater than or equal to 1.0 mgd;2)POTWs with a pretreatment program(or those that are
required to have one under 40 CFR Part 403);or 3)POTWs required by the permitting authority to submit data for these parameters.
• At a minimum,these results must include quarterly testing for a 12-month period within the past 1 year using multiple species(minimum of two
species),or the results from four tests performed at least annually in the four and one-half years prior to the application,provided the results
show no appreciable toxicity,and testing for acute and/or chronic toxicity,depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition,this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition,submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity,provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation,if one was conducted.
• If you have already submitted any of the information requested in Part E,you need not submit it again. Rather,provide the information
requested in question E.4 for previously submitted information. If EPA methods were not used,report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below,they may be submitted in place of Part E.
If no biomonitoring data is required,do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
complete.
E.1. Required Tests.
Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
chronic 0 acute Four Fathead Minnow(second species)tests
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one
column per test(where each species constitutes a test). Copy this page if more than three tests are being reported.
Test number: 4 Test number: N/A Test number: N/A
a. Test information.
na
Test Species&test method number M82-R o2 01 i:i
EPA-82-R-02-014:1006
Age at initiation of test 10 days
Outfall number 002
Dates sample collected 2/11/18—2/16/18
Date test started 2/12/2018
Duration 7 days
b. Give toxicity test methods followed.
Short-term Methods for Estimating the
Manual title Chronic Toxicity of Effluentts and
Receiving Waters to Marine and
Estuarine Organisms
Edition number and year of publication 3rd Edition,2002
Page number(s) All
c. Give the sample collection method(s)used. For multiple grab samples,indicate the number of grab samples used.
24-Hour composite X
Grab
d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection X
After dechlorination
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 18 of 20

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
City of Havelock WWTP, NC0021253 Renewal Neuse River
Test number: 3 Test number: N/A Test number: N/A
e. Describe the point in the treatment process at which the sample was collected.
Sample was collected: Final Effluent
f. For each test,include whether the test was intended to assess chronic toxicity,acute toxicity,or both
Chronic toxicity X
Acute toxicity
g. Provide the type of test performed.
Static
Static-renewal X
Flow-through
h. Source of dilution water. If laboratory water,specify type;if receiving water,specify source.
Laboratory water Artificial Salt Water
Receiving water
i. Type of dilution water. If salt water,specify"natural"or type of artificial sea salts or brine used.
Fresh water
Salt water Instant Ocean Sea Salt
j. Give the percentage effluent used for all concentrations in the test series.
3.75,7.5, 15,30, 60
k. Parameters measured during the test. (State whether parameter meets test method specifications)
pH X/Yes
Salinity X/Yes
Temperature X/Yes
Ammonia
Dissolved oxygen X I Yes
I. Test Results.
Acute:N/A
Percent survival in 100% ,�o "�u
effluent
LCso
95%C.I. % %
Control percent survival
Other(describe)
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 19 of 20

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
City of Havelock WWTP, NC0021253 Renewal Neuse River
Chronic:
NOEC 30%
1C25 N/A
Control percent survival 100%
Other(describe)ChV
(Chroic Value) 42.4%
m. Quality Control/Quality Assurance.
Is reference toxicant data available? Yes
•
Was reference toxicant test within
acceptable bounds? Yes
What date was reference toxicant test 2/12/2018
run(MM/DD/YYYY)?
Other(describe) N/A N/A
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ® No IfY es,describe:
E.4. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information,or information regarding the
•
cause of toxicity,within the past four and one-half years,provide the dates the information was submitted to the permitting authority and a summary
of the results. N/A
Date submitted: / / (MM/DD/YYYY)
Summary of results: (see instructions)
END OF PART E.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 20 of 20

City of Havelock Wastewater Treatment Plant
City of Havelock, North Carolina
Second Species Toxicity Testing Results 2017-2018

i
I
env rnnm ,Inc. (E64)B77.6942 .FAX[phone]
P,0, ®ax 16414, Greenvale, SC 28606 4 Craftsman Court,Greer,SC 29650
l
Menidia beryllina Survival & Growth Test
EPA-821-R-02-014 Method 1006
•
1 Permit Renewal: Second Species Testing
Client: CITY OF HAVELOCK
Facility:WWTP
NPDES#:NC0021253
Test Date: 30-Nov-17
Laboratory Sample ID#:T50492
Test Reviewed and Approved By:
Robert W.Kelley,Ph.D. Farhad Rostampour
QA/QC Officer Laboratory Director
(
- Certification#E87819 SCDHEC Certification#23104
Test results presented in this report conform to all requirements of
NELAC,conducted under NELAC Certification Number E87819
Florida Dept.of Health.Included results pertain only to provided samples. NCDENR Certification# 022
Page 1 of 6

i
Effluent Toxicity Report Form-Chronic Menidia beryllina Mutti-Concentration Test
Facility: Havelock NPDES# NC0021253 Pipe#: 001 County: Craven
Laboratory: ETT Environmental, Inc. Comments
x
Signature of Operator in Responsible C rg
x ..---------_,,...,„7„-----,_„,_..----/ -y/
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Environmental Sciences Branch
Division pf Water Quality
NC DENR
1621 Mal Service Center
Raleigh, VC 27699-1621
Test Initiation Date/Time 11/30/17 / 02:00 PM Avg Wt/Surv.Control 1.6438 Test Organisms
%Eff. Repl. 1 2 4 4 Cultured In-House
Control S rviving# 10 8 9 10 %Survival
97.4% X Outside Supplier
ri in i
g a # 10 8 10 10
Wt/ori inal(mg)
1 6730 1.8613
( 9) 1.4850 1.3910 Avg Wt(mg) 1.6026 Hatch Date: 11/20/17
3.8 S rviving# 9 10 10 10 %Survival 100.0% Hatch Time: BATCH 2
riginal# 9 10 10 10
Wt/ori inal(mg) 1.6222 1.5650 1.6720 1.7960 Avg Wt(mg) 1.6638
7.5 S rviving# 10 10 10 10 %Survival 100.0%
riginal# 10 10 10 10
Wt/ori inal(mg) 1.8190 1.5590 1.4460 1.6150 Avg Wt(mg) 1.6098
15.0 S rviving# 10 10 10 10 %Survival 100.0%
riginal# 10 10 10 10
Wt/ori inal(mg) 1.5890 1.6600 1.4590 1.6150 Avg Wt(mg) 1.5808
30.0 S rviving# 10 10 10 10 %Survival 100.0%
Priginal# 10 10 10 10
Wt/ori.inal(mg) 1.5950 1.5670 1.4410 1.351C Avg Wt(mg) 1.4885
60.0 S rviving# 10 10 10 10 %Survival 100.0%
original# 10 10 10 10
Wt/ori.inal(mg) 1.3920 1.5340 1.4130 1.2840 Avg Wt(mg) 1.4058
Water Quality Da a Day
Control 0 1 2 3 4 5 6 7
pH(S )Init/Fin 8.2 / -- 8.2 / 7.9 8.3 / 7.8 8.0 / 7.8 8.2 / 7.9 8.2 / 8.4 8.0 / 7.5 ----/ 7.7
DO(mg/Li Init/Fin 6.5 1 - 6.7 / 6.1 6.8 / 5.3 5.6 / 5.2 6.5 / 5.5 6.8 / 4.9 6.7 / 5.6 ----/ 4.7
Temp(4)Init/Fin 24.7 / - 24.5 / 24.7 24.6 / 24.6 25.0 I 25.3 24.9 I 25.5 24.8 / 25.2 24.7 / 25.2 ----/ 24.8
Salinity li.pt)I/F 25.0 / ---- 26.1 / 26.4 26.3 / 26.5 26.9 / 26.6 27.2 / 26.4 27.5 / 26.3 27.2 / 26.0 ----/ 29.5
High Concentratl•n 0 1 2 . 3 4 5 6 7
pH(S )'nit/Fin 8.3 / - 8.4 / 8.3 0.0 / 8.3 8.3 / E.2 8.3 / 8.2 8.3 / 8.2 8.1 / 8.1 ----/ 8.3
DO(mg/L Init/Fin 6.6 / -- 6.5 / 5.3 6.6 / 5.5 6.5 / °.4 6.6 / 5.5 6.9 / 5.3 6.9 / 5.9 ----/ 5.2
Temp(S)'nit/Fin 25.0 / - 25.1 / 24.7 24.8 / 24.6 24.9 / 26.3 25.2 / 25.5 25.4 / 25.2 25.9 / 252 ----/ 24.8
Salinity(p.t)InitTin 25.8 / 26.7 / 23.8 27.3 / 26.3 27.4 / 25.5 27.6 / 25.7 27.3 / 26.3 27.2 / 24.4 / 29.0
Sample 1 2 Survival Growth Overall Result
Collection S art Date 11/28/17 11/30/17 12/05/17 Normal no yes ChV >60.00%
Grab Horn.Var. yes yes
Composite(Duration) 24 hr 24 hr 24 hr NOEC 60.0% 60.0%
Hardnes. (mg/L) 100.0 110.0 120.0 LOEC >60.00% >60.00%
Alkalini q (mg/L) 174.6 215.5 258.0 ChV >60.00% >60.00%
Conductivity(u hos/cm) 846 824 856 Method Steel's Test T-Test
Chlori e(mg/L) <0.05 0.05 <0.05
Temp at Recei.t(C) 0.0 1.7 0.1 Slats Survival Growth
not frozen Conc. Critical Calculated Critical Calculated
Dilut on H2O 3.8% 10.00 20.00 2.41 -0.64
Hardnes. (mg/L) 46.0 7.5% 10.00 20.00 2.41 -0.08
Alkalinit, (mg/L) 28.6 15% 10.00 20.00 2.41 0.23
Conductivity(urn os/cm) 190 30% 10.00 20.00 2.41 1.20
60% 10.00 20.00 2.41 2.07
DWQ Form AT-5(/04)
Page 2 df 6

Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Weight Fecundity
source rep Dead Lost Deed Lost Dead Lost Dead Lost Dead Lost Dead Lost Dead Lost nig N-Gray Gravid Survival Lab# T50492
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16.73 10 Client Havelock
B 0 0 0 0 0 0 0 1 0 0 0 0 0 1 14.89 8 Sample ID WWTP
C 0 0 0 0 0 0 0 0 1 0 0 0 0 0 14.85 9 NPDES# NC0021253
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13.91 10 County Craven
`tttrol E 0 Month 11
F 0 Start&fed Date 30-Nov-17
G 0 Start&ted Time 02:00 PM
H 0, Started&ted By AM
A 0 0 0 0 0 0 0 0 0 0 0 0 0 1 14.6 9 Test Organism Menidia beryilina
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.65 10 Neo.born date 20-Nov-17
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16.72 10 Nee.born time BATCH 2
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0, 17.96 10 Test Type NCCD
3.75 E 0 Dilution Water INSTANT OCEAN
F 0 units for Conc.
G 0 143rd BROOD
H _ 0 Test vessels 400 nil
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18.19 10 Test volume 150 ml
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.59 10 Incubator# 1
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.46 10 Light 16It/8dk
D 0 0 0 0 0 0 0 0 0 0 0, 0 0 0 16.15 10 initial Temp•C 25
7.5 E 0 Artemla 750
F 0 Test method EPA 621-R-02-013:1006
G 0
H0
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.89 10
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16.6 10
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.59 10
D 0 0 0 0 0 0 0 0 0 0 0 C 0 0 16.15 10
15 E 0
F 0 Comments
G 0 All reps have 10
H _ 0 organisms unless noted otherwise.
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15,95 10
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.67 10 Control in'temp
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.41 10
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13.51 10
30 E 0
F 0
G 0
i H 0
A 0 0 0 0 0 0J 0 0 0 0 0 C 0 C 13.92 10
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.34 10
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.13 10
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12.84 10
60 E 0
F 0
G 0
Ht 0
Renew JG JG FJ FJ FJ FJ ' End Date
Fed JO JG FJ FJ FJ FJ
Time fed 8 wow 01:54 PM 11:31 AM 12 34 PM C3:C6 PM C4.01 PM 05.09 PM I 07-Dec-17 JG
Effluent New tame.•0 25.1 24.8 24.9 25.2 25.4 25.9 02:10 PM
and I.mp.•0 24.7 24.6 25.3 25.5 25.2 25.2 24.81 D=Dead N/A-Lost or not used
Control Haw temp.•C 24.5 24.6 25.0 24.9 24.8 24.7
T
Page 3 of 6

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PO Box 16414,Greenville,SC 29606-7414
[phone], [phone] Farc(864)877 6938
Shipping Address:4 Craftsman Ct.,Greer,SC 29650
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE MONITORING PROCEDURES HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours.Equal vole 0.0 and 6.0°C.Samples must not be frozen.Use water ice in sealed bas. of sample collection(completion of composite sample).
or at minimum I sample every 4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow Pro/ . 11:As per instructions in N\NPDES permit.

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State: L NPDES 4: C i�r ` Organisms' —
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE MONITORING PROCEDURES
Composite samples must be collected over a 24 HOLD TIME PROCEDURES
P Phour period. Sample temperature during collection and transport must be between For toxicity testing the sample must fust be used within 36 hours
Time Proportional:I sample each hour for 24 hours.Equal volut 0.0 and 6.0`C.Samples must not be frozen.Use water ice in sealed bags.
of sample collection(completion of composite sample).
or at minimum I sample every 4 hours over 24 hours.
Flow Proportional:As per instructions in NPDES permit.
Sample may not be used after 72 hours from sample collection.

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CI]AIN OF CUSTODY RECORD
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PO Box 16414,Greenville Sc 29606-7414 [Page T of
[phone], (800)891-4j25 Far(8e4)877 69 8 __ tt
miump.- Shipping Address:4 Craftsman Ct,Greer,SC 29650
WWw_CrTCNVIRO N IN CNTAL.COM -
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Facility 1�(7[C�_ ' (�'��^
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE MONITORING PROCEDURES HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must first be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours.Equal volut 0.0 and 6.0°C.Samples must not be frozen.Use water ice in scaled bags. of sample collection(completion of composite sample).
or at minimum 1 sample even'4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow Proportional:As per instructions in NPDES permit.

sm Inc. [phone] . FAX(864)8'7.6938
P.O. Box 16414, Greenvlie, SC 29606 4 Craftsman Cain, Greer, SC 29650
Menidia beryllina Survival & Growth Test
EPA-821-R-02-014 Method 1006
Permit Renewal: Second Species Testing
Client: CITY OF HAVELOCK
Facility:WWTP
NPDES#:NC0021253
Test Date: 12-Dec-17
Laboratory Sample ID#: T50606 MENIDIA
Test Reviewed and Approved By:
tai P
Robert W.Kelley,Ph.D. Farhad Rostampour
QA/QC Officer Laboratory Director
Certification#E87819 SCDHEC Certification#23104
Test results presented in this report conform to all requirements of
NELAC,conducted under NELAC Certification Number E87819
Florida Dept.of Health.Included results pertain only to provided samples. NCDENR Certification# 022
Page 1 of 6

Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test
Facility: Havelock NPDES# NC0021253 Pipe#: 001 County: Craven
Laboratory: ETT Environmental, Inc. Comments
x
Signature of Operator in Responsible Charge
x
Signature of Laboratory Supervisor I
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh,NC 27699-1621
Test Initiation Date/Time 12/12/17 / 02:53 PM AvgWt/Surv. Control 1.2786 Test Organisms
9
%Eff. Repl. 1 2 3 4 Cultured In-House
Control Surviving# 10 10 9 10 %Survival 97.5% X Outside Supplier
Original# 10 10 10 10
Wt/original(mg) 1.3290 1.2320 1.1290 1.2990 Avg Wt(mg) 1.2473 Hatch Date: 12/03/17
3.8 Surviving# 10 10 9 9 %Survival 95,0% Hatch Time: 12:00 AM
Original# 10 10 10 10
Wt/original(mg) 1.3980 1.1950 1.1560 1.1600 Avg Wt(mg) 1.2273
7.5 Surviving# 10 9 10 10 %Survival 97.5%
Original# 10 _ 10 10 10
Wt/original(mg) 1.3780 1.2650 1.2710 1.3570 Avg Wt(mg) 1.3178
15.0 Surviving# 10 10 10 8 %Survival 95.0%
Original# 10 10 10 10
Wt/original(mg) 1.1020 1.0680 0.9480 1.1870 Avg Wt(mg) 1.0763
30.0 Surviving# 10 10 10 10 %Survival 100.0%
Original# 10 10 10 10
Wt/original(mg) 1.0540 1.0210 1.1390 1.0130 Avg Wt(mg) 1.0568
60.0 Surviving# 8 10 10 10 %Survival 95.0%
Original# 10 10 10 10
Wt/original(mg) 0.9620 1.0720 0.9320 1.2210 Avg Wt(mg) 1.0468
Water Quality Data Day
Control 0 1 2 3 4 5 6 7
pH (SU)Init/Fin 8.1 / - 8.1 / 8.0 8.0 / 8.4 8.0 / 8.0 8.0 / 8.2 8.0 / 7.8 8.1 / 7.7 ----/ 8.2
DO(mg/L) Init/Fin 6.2 / - 7.2 / 6.4 7.5 / 5.3 6.8 / 5.8 6.7 / 6.0 6.8 / 5.9 5.8 / 4.4 ----/ 4.6
Temp(C)InitlFin 24.7 / - 24.7 / 24.8 24.6 / 24.5 24.8 / 25.0 25.2 / 25.7 25.5 / 25.5 25.3 / 25.8 ----/ 26.0
Salinity 26.7 25.4 / 27.1 25.5 / 27.3 25.4 / 26.9 25.6 / 26.8 25.6 / 26.9 25.6 / 26.3 26.4
High Concentration 0 1 2 3 4 5 6 7
pH (SU)Init/Fin 8.3 / -_ 8.0 / 8.1 _ 8.2 / 8.4 8.1 / 8.2 8.1 / 8.2 8.1 / 8.3 _ 8.1 / 8.2 ----/ 8.4
DO(mg/L) Init/Fin 6.9 / - 7.0 / 6.1 7.2 / 5.9 6.6 / 5.6 _ 6.5 / 5.7 6.7 / 5.9 5.7 / 4.8 _----/ 5.0
Temp(C)Init/Fin 25.0 / - 24.7 / 24.8 24.9 / 24.5 24.8 / 25.0 24.9 / 25.7 24.8 / 25.5 25.2 / 25.8 ----/ 26.0
Salinity 25.6 25.6 / 27.4 25.0 / 26.4 24.9 / 26.2 25.2 / 26.5 25.3 / 26.7 25.3 / 26.7 27.2
Sample 1 2 3 Survival Growth Overall Result
Collection Start Date 12/10/17 12/12/17 12/14/17 Normal no yes ChV 10.6%
Grab Hom.Var. yes yes
Composite(Duration) 24 hr 24 hr 24 hr NOEC 60.0% 7.5%
Hardness(mg/L) 100.0 110.0 120.0 LOEC >60.00% 15.0%
Alkalinity(mg/L) 219.7 160.5 249.8 ChV >60.00% 10.6%
Conductivity(umhos/cm) 664 691 740 Method Steel's Test T-Test
Chlorine(mg/L) <0.05 <0.05 <0.05
Temp at Receipt(C) 0.1 0.1 0.3 Stats Survival Growth
Conc. Critical Calculated Critical Calculated
Dilution H2O 3.75% 10.00 16.00 2.41 0.30
Hardness(mg/L) 46.0 7.5% 10.00 18.00 2.41 -1.04
Alkalinity(mg/L) 28.6 15% 10.00 17.50 2.41 2.53
Conductivity(umhos/cm) 190 30% 10.00 20.00 2.41 2.82
60% 10.00 17.50 2.41 2.97
DWQ Form AT-5(1/04)
Page 2 of 6

I Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Weight Fecundity
source rep Dead Lost Dead Lost Dead Lost Dead Lost Dead Lost Dead Lost Dead Lost mg N-Gray Gravid Survival Lab& T50606 MENIDIA ,
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1329 10 Client Havelock ,
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12.32 10 Sample lD WWTP
C 1 0 0 0 0 0 0 0 0 0 0 0 0 0 11.29 9 NPDES# NC0021253
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12.99 10 County Craven
control E 0 Month 12
F
G - 0 Start&fed Date 12-Dec-17
0 Start&fed Time 02:53 PM
' H 0 Started&fed By JG
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13.98 10 rest Organism Menidia beryllina
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11.95 10 Neo.born date 03-Dec-17
C 0 0 0 0 0 0 0 0 0 0 0 0 1 0 11.56 9 Neo.born time 0
D 1 0 0 0 0 0 0 0 0 0 0 0 0 0 11.6 9 Test Type NCCD
3,75 E 0 Dilution Water INSTANT OCEAN
F 0 Units for Conc. e/d
' G 0 %3rd BROOD
H 0 Test vessels 400 ml
A 0 0 0 0 0 0 0 0_ 0 0 0 0_ 0 0 13.78 10 Test volume 150 ml
B 0 0 0 0 1 0 0 0 0 0 0 0 0 0 12.65 9 Incubator& 1
C 0 0 0 0 0 0 0 0 0 0 0 0. 0 12.71 10 Light 16IU8dk
D 0 0 0 0 0 0 0 0 0 0 0 0._ 0 0 13.57 10 Initial Temp°C 25
7.5 E _ 0 Artemia see below
F 0 Test method EPA 821-R-02-014:1006
G . 0
H 0
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11.02 10
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10.68 10
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9.48 10
D 2 0 0 0 0 0 0 0 0 0 0 0 0 0 11.87 8
15 E 0
F 0 Comments
G 0 All reps have 10
H 1 0 organisms unless noted otherwise.
A 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 10.54 10�
B 0 0 0 0 0 0 0 0 0 0_ 0 0 0 0 10.21 10 Control ini temp
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11.39 10
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10.13 10 ORGANISMS RECEIVED FROM ABS
30 E _ 0 Feeding:0.1 g/rep/day on Days 1-2
F _ 0 0-15 g/rep/day on days 3-6
G 0
H 0
A 0 0 0 0 1 0 0 0 0 0 0 0 1 0 9.62 8
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0_ 10.72 10
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9.32 10
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12.21 10
60 E _ 0
F 0
G 0
H 0
Renew JC JG JC FJ JG FJ End Date
Fed JC JG JC FJ JG FJ
Time fed&renew 03'01 PM 03:33 PM 02.20 PM 02:00 PM 08'.29 AM 0335 PM I 19-Dec-17 AM
Effluent New temp•c 24.7 24.9 24.8 24.9 24.8 25.2 026t PM
Old temp•c 24.8 24.5 25 25.7 25.5 25.8 261 D=Dead N/A-Lost or not used
Control New temp'c 24.7 24.6 24.8 25.2 25.5 25.3
Page 3 of 6

ET,jc,
CHAIN OF CUSTODY RECORD
i ai- Page ; of E
PO Box 16414,Greenville,SC 29606-7414
[phone], [phone] Fax:[phone]
Shipping Address:4 Craftsman Ct,Greer,SC 29650
W W W.ETTENVIRONMENTAL.COM
Client: �/
~ Program Containers Preservative
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Date Time Relinquished By/Organization Received By/Organization Area Temp°C Preserved?
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE MONITORING PROCEDURES HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must first be used within 36 hours
Time Proportional: l sample each hour for 24 hours.Equal volut 0.0 and 6.0°C.Samples must not be frozen.Use water ice in sealed bags. of sample collection(completion of composite sample).
or at minimum I----le every 4 hours over 24 hours. Sample may not be used after 72 hours from samnie collection.
Flow Proportion; ler instructions in NPDES permit.

ETrli
CHAIN OF CUSTODY RECORD
PO Box 16414,Greenville,SC 29606-7414
[phone], [phone] Fax_(864)877 6938
Shipping Address:4 Craftsman Ct,Greer,SC 29650
W WW.ETTENVIR.O�NM-ENTAL.00M
Client: l d t Jt IOC 1LT
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NPDES`: Acute Chronic Test Organisms
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Sample Custody Transfer Record Secure Receipt Sample
Date Time t- in uished By/�aOrganization Received By/Organization Area Temp'C � Preserved?
�►� 0 �$ Feder 1 A I I' , "
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE MONITORING PROCEDURES HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing.the sample must tint be used within 36 hours
Time Proportional: I sample each hour for 24 hours.Equal volut 0.0 and 6.0°C.Samples must not be frozen.Use water ice in sealed bags. of sample collection(completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow Proportional:As per instructions in NPDES permit.

ETrii
CHAIN OF CUSTODY RECORD
ifliiiiit Page 1 o; /
PO Box 16414,Greenville,SC 29606-7414
[phone], [phone] Fax:[phone]
Shipping Address:4 Craftsman Ct,Greer,SC 29650
W WW.ETTENVIRONMENTAL.COM
Client: r.-, /
C-L 0`7-- > i pt-7/ec'/r Program Containers Preservative Parameters
Facility: i ' ` y ,
G4�� f1c?�C /1 64t)n- Qt' �/ -2-7fr Whole EffluentTociciiy
State: ,,0/ ... NPDES#: Az:C.=? 4.23-7 Acute Chronic Test Organisms —
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the dotted line = E U o ^ ==t-tno: — „ E _ —
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SAMPLE ID Composite Start Date l Time Sample Collection Date Time Collected by U ✓. Z a E U > — < . U n G s - —I c Chemical Analysis&Other
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Sample Custody Transfer Record Secure Receipt Sample
Date Time Relit led tor,raanization Received By/Organization Area Temp 5C Preserved?
—
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COMPOSITE SAMPLING PROCEDURES TEiIIPER4TURE il•IONITORIATG PROCEDURES HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must firs be used within 36 hours
Time Proportional: I sample each hour for 24 hours.Equal wolut 0.0 and 6.0°C.Samples must not be frozen.Use water ice in sealed bags. of sample collection(completion of composite sample).
or at minimum I camnle every 4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow Proportion: >er instructions in NPDES permit.

N
Err.. ....c.
E64 877-6942.FAX 864 B77-6838
6938
P,O.box 1E414. GreenviI . SC 29606 4 Craftsman Court, Greer, SC 29650
Menidia beryllina Survival & Growth Test
EPA-821-R-02-014 Method 1006
Permit Renewal: Second Species Testing
Client: CITY OF HAVELOCK
Facility:WWTP
NPDES#:NC0021253
Test Date: 23-Jan-18
Laboratory Sample ID#: T50798 M beryllina
Test Reviewed and Approved By:
Robert W.Kelley,Ph.D. Farbad Rostampour
QA/QC Officer Laboratory Director
Certification#E87819 SCDHEC Certification#23104
Test results presented in this report conform to all requirements of
NELAC,conducted under NELAC Certification Number E87819
Florida Dept.of Health.Included results pertain only to provided samples. NCDENR Certification# 022
Page 1 of 6

Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test
Facility: Havelock NPDES# NC0021253 Pipe#: 001 County: Craven
Laboratory: ETT Environmental,Inc. Comments!
x
Signature of OperatorR o sib) Chalg
xfL Wil: ``
Signature of Laboratory Supervisor 1
`MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh,NC 27699-1621
Test Initiation Date/Time 01/23/18 / 01:00 PM Avg WUSurv. Control 1.6013 Test Organisms
%Eff. Repl. 1 2 4 4 Cultured In-House
Control Surviving# 10 10 10 10 %Survival 100.0% X Outside Supplier
Original# 10 _ 10 10 10
Wt/original (mg) 1.3070 1.2490 1.4160 1.4160 Avg Wt(mg) 1.3633 Hatch Date: 01/13/18
3.8 Surviving# 10 10 10 10 %Survival 100.0% Hatch Time: 0000-2400
Original# 10 10 10 10
Wt/original(mg) 1.1770 1.3720 1.4630 1.4540 Avg Wt(mg) 1.3665
7.5 Surviving# 10 10 10 10 %Survival 100.0%
' Original# 10 10 10 10
Wt/original (mg) 1.3050 1.4670 1.4600 1.5680 Avg Wt(mg) 1.4500
15.0 Surviving# 10 10 10 10 %Survival 100.0%
Original# 10 10 10 10
Wt/original(mg) 1.2920 1.2990 1.4290 1.3630 Avg Wt(mg) 1.3458
30.0 Surviving# 10 9 10 10 %Survival 100.0%
I Original# 10 9 10 10
Wt/original(mg) 1.1720 1.1356 1.2070 1.1970 Avg Wt(mg) 1.1779
60.0 Surviving# 10 10 10 10 %Survival 100.0%
Original# 10 10 10 10
Wt/original(mg) 1.2160 1.2920 1.3570 1.5530 Avg Wt(mg) 1.3545
Water Quality Data Day
Control 0 1 2 3 4 5 6 7
� pH(SU)Init/Fin 8.0 / - 8.2 / 8.0 8.2 / 8.2 8.3 / 8.0 8.2 / 7.8 8.3 / 7.4 8.1 / 8.0 ----/ 8.3
DO(mg/L) Init/Fin 7.0 / - 6.8 / 5.8 6.6 / 5.3 6.3 / 5.1 6.2 / 4.9 7.2 / 5.6 6.8 / 5.2 ----/ 4.7
Temp(C)Init/Fin 24.7 / - 25.0 / 25.6 25.2 / 25.8 24.6 / 24.7 24.5 / 24.6 24.8 / 24.8 24.6 / 25.8 ----/ 24.9
Salinity 24.7 24.8 / 25.2 25.0 / 25.3 24.6 / 25.3 24.9 / 25.4 25.0 / 25.5 25.6 / 26.7 25.8
High Concentration 0 1 2 3 4 5 6 7
pH(SU)Init/Fin 8.1 / - 8.1 / 8.2 8.1 / 8.2 8.2 / 8.3 8.1 / 8.3 8.0 / 8.4 8.2 / 8.2 ----/ 8.2
DO(mg/L) Init/Fin 6.5 / - 6.6 / 5.9 6.4 / 5.4 6.7 / 5.6 7.2 / 5.9 6.0 / 6.3 6.8 I 6.2 ----/ 5.6
Temp(C)Init/Fin 25.0 / - 25.1 / 25.6 25.3 / 25.8 24.9 / 24.7 24.8 / 24.6 24.9 / 24.8 25.1 / 25.8 ----/ 24.9
Salinity 24.2 24.9 / 27.7 24.2 / 25.5 24.6 / 25.5 24.7 / 25.6 24.8 / 25.6 25.5 / 27.1 25.3
Sample 1 2 Survival Growth Overall Result
Collection Start Date 01/21/18 01/23/18 01/25/18 Normal no yes ChV >60.0%
Grab Horn.Varyes yes
Composite(Duration) 24 hr 24 hr 24 hr NOEC 60.0% 60.0%
Hardness(mg/L) 120.0 100.0 110.0 LOEC >60.0% >60.0%
Alkalinity(mg/L) 186.7 206.0 193.5 ChV >60.0% >60.0%
Conductivity(umhos/cm) 783 728 724 Method Steel's Test T Test
Chlorine(mg/L) 0.09 <0.05 <0.05
Temp at Receipt(C) 0.1 0.1 1.8 Stats Survival Growth
Conc. Critical Calculated Critical Calculated
Dilution H2O 4% 10.00 18.00 2.41 -0.04
Hardness(mg/L) 46.0 8% 10.00 18.00 2.41 -1.17
Alkalinity(mg/L) 192.0 15% 10.00 18.00 2.41 0.24
Conductivity(umhos/cm) 30.7 30% 10.00 18.00 2.41 2.49
60% 10.00 18.00 2.41 0.12
DWQ Form AT-5(1/04)
Page 2 of 6

Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Weight Fecundity
source rep Dead Lost Deed Lost Dead Lost Dead Lost Deed Lostpeed Lost Deed Lost mg N-Gray Gravid Survival Lab# T50798 M berylllna
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13.07 10 Client Havelock
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12.49 10 Sample ID WWTP
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.81 10 NPDES# NC0021253
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.16 10 County Craven
rttro! E 0 Month 1
F 0 start a red Dote 23-Jan-18 _
G 0 Start&fed Time 01:00 PM
H 0 started a fed By JC
_ _
A 0 0 0 0 0 0 0 0 0 0 0 C 0 0 11.77 10 Test Organism MENIDIA BERYLLINi
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13.72 10 Neo.born date 13-Jan-18
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.63 10 Neo.born time BATCH 2
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.54 10 Test Type NCCD
3.75 E 0 Dilution Water INSTANT OCEAN
F 0 units for Conc. %
G 0 %Ord BROOD
H 0 Test vessels 1000 ml
A 0 0 0 0 0 C 0 0 0 0 0 0 0 0 13.05 10 Test volume 500 mL
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.67 10 Incubator 1
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.6 10 Light 16it/8dk
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.68 10 Initial Temp'C 25
7.5 E 0 Artemis see below
F 0 Test method EPA e21-1,02-olt moos
G 0
H 0
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12.92 10
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12.99 10
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.29 10
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13.63. 10
15 E 0
F 0 Comments
G 0 All reps have 10
H 0 organisms unless noted otherwise.
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11.72 10
B 0 0 0 0 0 0 0 0 0 0 0 0 0, 1 10.22 9 Control inl temp iimac, 24.7
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12.07 10
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11.97 10
30 E 0 ORGANISMS
F 0 SOURCE=ABS 1/23/18
G 0 10 DAYS OLD
H 0
A 0 0 0' 0 0 0 0 0 0 0 0 0 0 0 12.16 10 Feeding:0.1 g/rep/day day 0-2
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12.92 10 0.15 g/rep/day day 3-6
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13.57 10
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.53 10
60 E 0
F 0
G 0
H 0
Renew FJ FJ JG JG JG AM End Date ,
Fed FJ FJ JG JG JG AM
Toner..l renew 03.50 PM 03.12 PM 03:12 PM 1'..05 AM '.o.19 AM 01.53 PM ( 30-Jan-18 JC
Effluent New lamp•c 25.1 25.3 24.9 24.8 24.9 25.1 c1.24 PM
Oa temp-c 25.6 25.8 24.7 24.6 24.8 25.8 24.91 D=Dead N/A-Lost or riot used
Carmol New temps 25.0 25.2 24.6 24.5 24.8 24.6
Page 3 of 6

]ET' _,
1 CHAIN OF CUSTODY RECORD
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Paye o`
PO Box 16414,Greenville,SC 29606-7414
[phone], [phone] Fax:[phone]
Shipping Address:4 Craftsman Ct,Greer,SC 29650
Www.ETTEN V IR❑NM ENTAL.0❑M
Client: r ,/ r-,
(_ i ryr G7 r /we,/C," Program Containers Preservative Parameters
Facility: ' //-- jj --^ L
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State: A/C NPD ES 4: /'t C /2553 Acute Chronic Test Organisms n
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Sample Custody Transfer Record Secure Receipt Sample
Date Time Relinquished By/Organization Received By/Organization Area Temp aC Preserved?
/4,2//s / ra ,,, r'��s/;� i^ /4/e/0:,� A� p p
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE ilIONITORING PROCEDURES HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours.Equal voles 0.0 and 6.0'C.Samples must not be frozen.Use water ice in sealed bas. of sample collection(completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow \s per instructions in NPDES permit. 4

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PO Box 16414,Greenville,SC 29606-7414
[phone], [phone] Fa (864)877 55938
Shipping Address:4 Craftsman Ct,Greer,SC 29650
W W W.ETTEN V I R D N M E NTAL.CO M
Client: ,46, 1, //
C_�j 7 c ir&�o-cA Program Containers Preservative Parameters
Facility: o. L J
c r4 " G3,4t' /�' /- e%/%. P- ,-ss'7' Whole Effluent Toxicity
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(Composite only) (Grab or Composite) < '—o" (3 = C)6I'_- `- o = Z
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Sample Custody Transfer Record Secure Receipt Sample
Date Time Relinquished By/Organization Received By I Organization Area Tern.°C Preserved?
// 4/yam /F70,,- 7-,-4571--) oc /4,,e/e,r &2 7 P I -
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE MONITORING PROCEDURES HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours.Equal voles 0.0 and 6.0°C.Samples must not be frozen.Use water ice in sealed bags. of sample collection(completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow Proportional:As per instructions in NPDES permit.

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CHAIN OF CUSTODY RECORD
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PO Box 16414,Greenville,SC 29606-7414
[phone], [phone] Fax=[phone]
Shipping Address:4 Craftsman Ct,Greer,SC 29650
WWW.ETf ENVIFCNMENTAL.COM
Client:(' T �r /7 y
� t/ R�`p OC � Program Containers Preservative Parameters
Facility: / 2r � 9
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Whole Effluent Toxicity }
State: A,/,/' NPDESc
l�C QV(916 ,a Acute Chronic Test Organisms
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/�e�fi jf aj�i� I���'�_' Ufa.,, i. 3 s c)I Y Q //mac ,c%:-.2
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Special Instructions:
Sample Custody Transfer Record Secure Receipt Sample
Date Time Relinquished By/Organization Received By!Organization Area Temp°C Preserved?
1426AKH/0 , /7 %2/es/,A/ r A Vg/Cocoe 0X'r? / _ I t;1
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE MONITORING PROCEDURES HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must first be used within 36 hours
Time Proportional:I sample each hour for 24 hours.Equal voles 0.0 and 6M°C.Samples must not be frozen.lise water ice in sealed bags. of sample collection(completion of composite sample).
or at minimum I sample every 4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow Prop()" As per instructions in NPDES permit.

E1. T. (E64)877.6942 .FAX[phone]
P.O,eox 1.t 14,GreenvlIe,SC 28606 4 Craftsman Court,Greer,SC 29650
Menidia beryllina Survival & Growth Test
EPA-821-R-02-014 Method 1006
Permit Renewal: Second Species Testing
Client: CITY OF HAVELOCK
Facility:WWTP
NPDES#:NC0021253
Test Date: 13-Feb-18
Laboratory Sample ID#: T50948
I '
Test Reviewed and Approved By:
Robert W.Kelley,Ph.D. Farhad Rostampour
QA/QC Officer Laboratory Director
Certification#E87819 SCDHEC Certification#23104
Test results presented in this report conform to all requirements of
NELAC,conducted under NELAC Certification Number E87819
Florida Dept.of Health.Included results pertain only to provided samples. NCDENR CertlfiCatlon# 022
Page 1 of 6

Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test
Facility. Havelock NPDES# NC0021253 Pipe#: 001 County: Craven
Laboratory: ETT Environmental, Inc. Comments
x �*'Signature of OpereteF,ir+�2es risible C - •
Signature of_aboratory Supervisor J
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh,NC 27699-1621
Test Initiation Date/Time 02/13/18 / 02:00 PM Avg Wt/Surv. Control 1.7358 Test Organisms
%Eff. Repl. 1 2 4 4 Cultured In-House
Control Surviving# 10 10 10 10 %Survival 100.0% X Outside Supplier
Original# 10 10 10 10
Wt/orginal(mg) 1.7850 1.6590 1.5410 1.9580 Avg Wt(mg) 1.7358 Hatch Date: 02/03/18
3.8 Surviving# 10 9 10 10 %Survival 100.0% Hatch Time: 12:00 AM
Original# 10 9 10 10
Wt/orginal (mg) 1.7310 1.6833 1.7690 1.6530 Avg Wt(mg) 1.7091
7.5 Surviving# 10 10 10 10 %Survival 100.0%
Original# 10 10 10 10
Wt/orginal(mg) 1 5360 1.7410 1.5810 1.7890 Avg Wt(mg) 1 6618
15.0 Surviving# 10 10 8 10 %Survival 97.4%
Original# 10 10 9 10
Wt/orginal(mg) 1.5640 1.5160 1.2778 1.6440 Avg Wt(mg) 1.5005
30.0 Surviving 4 10 10 10 10 %Survival 100.0%
Original# 10 10 10 10
WUorginal (mg) 1.5800 1.4340 1.6420 1.50'0 Avg Wt(mg) 1.5408
60.0 Surviving# 10 10 10 10 %Survival 100.0%
Original# 10 10 10 10
Wt/orginal(mg) 1.6550 1.3510 1.6360 1.3620 Avg Wt(mg) 1.5010
Water Quality Data Day
Control 0 1 2 3 4 5 6 7
pH(EU)Init/Fin 8.3 / - 8.2 / 7.9 8.5 / 8.6 8.1 / 7.7 8.1 / 7.6 8.1 / 7.7 8.5 / 8.4 ----/ 7.9
DO(mg/L) Init/Fin 6.5 / - 6.5 / 5.4 6.3 / 6.2 6.7 / 4.6 6.6 / 4.3 6.7 / 4.7 7.2 / 5.9 ----/ 5.1
Temp 'C)Init/Fin 24.7 / - 0.0 / 25.3 0.0 / 25.4 24.8 / 25.1 24.5 / 25.1 24.8 / 25.1 24.6 / 25.3 ----/ 25.4
Salinity 25.2 25.0 / 25.7 25.3 / 24.9 24.6 / 24.2 24.8 / 24.6 24.1 / 24.3 24.8 / 25.6 24.6
High Concentration 0 1 2 3 4 5 6 7
pH(SU)Init/Fin 8.2 / - 8.2 / 8.3 8.3 / 8.4 8.0 / 8.1 8.1 / 8.1 8.0 / 8.1 8.4 / 8.4 ----/ 8.2
DO(mg/L) Init/Fin 6.9 / - 6.8 / 5.6 7.0 / 5.4 6.8 I 5.1 6.8 / 5.3 6.9 / 5.1 7.6 / 6.7 ----/ 6.4
Temp(C)IniUFin 25.0 / - 24.9 / 25.3 24.9 / 25.4 25.0 I 25.1 25.2 / 25.1 24,8 / 25.1 24.9 / 25.3 ----/ 25.4
Salinity 25.9 25.8 / 28.2 24.5 / 26.6 24.1 / 26.9 25.0 / 26.5 24.4 / 25.2 24.5 / 25.0 25.8
Sample 1 2 Survival Growth Overall Result
Collection ptart Date 02/11/18 02/13/18 02/15/18 Normal no yes ChV 42.4%
Grab Hom.Var. yes yes
Composite(Duration) 24 hr 24 hr 24 hr NOEC 60.0% 30.0%
Hardn ss(mg/L) 112.0 110.0 118.0 LOEC >60.00% 60.0%
Alkali ity(mglL) 156.3 121.8 160.0 ChV >60.00% 42.4%
Conductivity( mhos/cm) 693 620 628 Method Steel's Test T-Test
Chio ine(mg/L) 0.06 <0.05 <0.05
Temp at Rec ipt(C) 0.1 0.9 2.3 Stats Survival Growth
Conc. Critical Calculated Critical Calculated
Oil tion H2O 4% 10.00 18.00 2.41 0.28
Hardness(mg/L) 4100.0 8% 10.00 18.00 2.41 0.77
Alkalinity(mg/L) 123.0 15% 10.00 16.00 2.41 2.45
Conductivity(umhos/cm) 49000 30% 10.00 18.00 2.41 2.03
60% 10.00 18.00 2.41 2.45
DWQ Form AT-5,(1/04)
Page 2 of 6

Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Weight Fecundity
source rep Deed Lost/lead Lost Dead Lost Deed Lost Dead Lost Deed Losl Deed Lost mg N-Grey Gravid Survival Labe T50948
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 19.44 10 Client Havelock
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16.59 10 Sample ID WWTP
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.41 10 NPDES# NC0021253
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 19.89 10 County Craven
,control E 0 Month 2
i 0
0 Start 8 ted Date 13-Feb-18
G 0 Start&fed Time 02:00 PM
H stoned a ted By JC
A 0 . 0 0 0 0 C 0 C 0 C 0i C 0 0 17.31 10 Teat Organism MENIDIA BERYLLIN
B 0 0 0 0 0 0 0 0 0 0 0 0 0 1 15.15 9 Nso.horn dote 03-Feb-18
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 17.69 10 Nee.born time 0
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16.53 10 Test Type NCCD
3.75 E 0 Dilution Water INSTANT OCEAN
F 0 Unita for Conc. %
G 0 %3rd BROOD
H 0 Test veutia 1000 ml
A 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.36 10 Test volume 500mL
B 0 0 0 0 0 0 0 0 0 0 0 0_ 0 0 17.41 10 ,Incubator It 1
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.81 10 Light 1611./8dk
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 17.89 10 Initial Temp'C 25
7.5 E 0 Artemia 0.10(0-2)/.150(3-61?xDAY
F 0 Test method EPA 82'.R-02-013.1000
G 0
H 0
A 0 0 0 0 0 0 0 0 0 00 0 0 0 0 15.64 10
B 0 0 0 0 0 C 0 0 0 0 0 0 0 0 15.06 10
C 0 0 1 0 0 0 0 0 0 0 0 0 0 1 11.01 8
o 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16.44 10
15 E 0
F 0 Comments
G 0 All reps have 10
H 0 organisms unless noted otherwise.
A of 0 0 0 0- 0- 0 of 0 0- 0 0 0 0' 10
B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14.34 10 Control ini temp III i
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16.42 10
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 15.07 10 ORGANISMS ARE 10 DAYS OLD •
30 E 0 SOURCE ABS 2/13/18
F0
G 0 I
H 0
A 0 0 0 0 0 0 0 0 C 0 C 0 0 0 16.55 10
B 0 0 0 0 0 0 0 0 0 0 0, 0 0 0 13.51 10
C 0 0 0 0 0 0 0 0 0 0 0 0 0 0 16.36 10
D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13.62 10
r '
60 E 0
F 0
G 0
H 0
Renew JG JG JC FJ JC JG End Date
tec JG JO JC FJ JC JO
Time tac6!anew 04 47P 0'e0 PM '.C.2'AM C4 2f1 PM 10.62 AM 0110PM 20-Feb-18 AM
Feist New temp•c 24.9 24.9 25.0 25.2 24.8 24.9 I 01 20 PM
OW 1000•c 25.3 25.4 25.1 25.1 25.1 25.3 25.41 ID=Deec N/A-Lost or not used
Control Now i.0o-c 24.8 24.5 24.8 24.6
Page 1 of 6

ETT
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CHAIN OF CUSTODY RECORD
PO Box 16414,Greenville,SC 29606-7414 `page p`_�
(8&4)877-&842--(66p}B31=2325-Faxt6n4)t[r1b936 - _. --- - --- _
111
Shipping Address:4 Craftsman Ct,Greer,SC 29650
W W W.ETTENVIRONMENTAL.COM
Client: 773 , j
7> ,C,7 - //4/le/bC../(--' Program Containers Preservative Parameters
Facility: i//cl � /7,e1„5,..„... t ESI —y�
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Whole Effluent TorieinState• NPDES�: i — �'
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Special Instructions:
Sample Custody Transfer Record
Secure Receipt Sample
Date Time Relin uished By/Organization Received By i Organization
r.� / Area Temp=C Preserved?
13 t 8 ogq S FedEx I , ; ETr--- ! cJ
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE d ION/TORING PROCEDURES HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours.Equal volut 0.0 and 6.0°C.Samples must not be frozen.use water ice in sealed bas. of sample collection(completion of composite sample).
or at minimum I sample every 4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
—
Flow Proportional:As per instructions in 1\TDES permit.

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CHAIN OF CUSTODY RECORD
-mmiameemAsii{; =a yr
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PC Box 6414,Greer-miller-SC-29606-7414 — --
(864) -6942, [phone] Fatc(864)877 6938 -- -- :._
Shipping Address:4 Craftsman CI,Greer,SC 29650 - - - - -
WWW.Err—ivreONNENTAL.COM
Client: / - _
L/� ©1r( /r{y'e4C// Program Containers Preservative Parameters
Facility: 1/-4,_.1.14e f '�/fir
ZG (iU77":,-, /,,-.-7�. LG7 yJ r' P‘....77(- Whole Lf7lucnt Tosicin•
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the doted line = U _E _ = =HNO3 s _ c
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SAMPLE ID Composite Senn Date Time Sample Collection Date Time Collected by U i- co Z a _ v > E G U J U ,,L" 2 r _ U ^ 3 Chemical Analysis&Other
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Special Instructions:
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Sample Custody Transfer Record Secure Receipt Sample
Date Time Relinquished By i Organization Received By i Organization Area Tema°C I Preserved?
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE MONITORING PROCEDURES HOLD TIME PROCEDU_RFS
Composite samples must be collected over a 24 hour period. Sample temperature during_collection and transport must be between For toxicity testing the sample must firs be used within 36 hours
Time Proportional: I sample each hour for 24 hours.Equal volut 0.0 and 6.0'C.Samples must not be frozen.lise water ice in sealed bags. of sample collection(completion of composite sample).
or at minimum I sample every 4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow Proportional:As per instructions in NPDES permit. -

TT , CHAIN OF CUSTODY RECORD __
teats7.-i'4...,4 €E c: �Pase _�of�_
PO Brno 16414,Greenville Sc 29606-7414 1
0364)877-66942;( 864)877-693E ---------
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WW W.ETTEN VIRO NM ENTAL.COM
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Sample Custody Transfer Record
Secure Receipt Sample
Date Time Relinquished By/Organization Received By/Organization Area Temp'C Preserved?
4./ P �14 ,Chary C�'r°ry- �
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COMPOSITE SAMPLING PROCEDURES TEMPERATURE MONITORING PROCEDURES HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must fires be used within 36 hours
Time Proportional: I sample each hour for 24 hours.Equal volur 0.0 and 6.0'C.Samples must not be frozen.Use water ice in sealed bas. of sample collection(completion of composite sample).
or at minimum I sample every 4 hours over 24 hours. Sample may not be used after 72 hots from sample collection.
Flow Proportional:As per instructions in NPDES permit.