NC0025453_Renewal Application_20170925

Waiter Resources
ENVIRONMENTAL QUALITY
October 02, 2017
Timothy Simpson, PE
Town of Clayton
PO Box 879
Clayton, NC 27520
Subject: Permit Renewal
Application No. NCO025453
Little Creek WWTP
Johnston County
Dear Applicant:
ROY COOPER
calor
MICHAEL S. REGAN
Secretary
S. JAY ZIMMERMAN
r7Vrrcrar
The Water Quality Permitting Section acknowledges the September 29, 2017 receipt of your permit renewal application
and supporting documentation. Your application will be assigned to a permit writer within the Section's NPDES WW
permitting branch. Per G.S. 15OB-3 your current permit does not expire until permit decision on the application is made.
Continuation of the current permit is contingent on timely and sufficient application for renewal of the current permit.
The permit writer will contact you if additional information is required to complete your permit renewal. Please respond
in a timely manner to requests for additional information necessary to allow a complete review of the application and
renewal of the permit.
Information regarding the status of your renewal application can be found online using the Department of Environmental
Quality's Environmental Application Tracker at:
https: //deg. nc.gov/permits-regulations/permit-guidance/environmental-a pplication-tracker
If you have any additional questions about the permit, please contact the primary reviewer of the application using the
links available within the Application Tracker.
Sincerely,
Wren Thedford 1
Administrative Assistant
Water Quality Permitting Section
cc: Central Files w/application
ec: WQPS Laserfiche File w/application
State of North Carolina I Environmental Quality I Water Resources
1617 Mail Service Center I Raleigh, North Carolina 27699-1617
919-807-6300

TOWN OF CLAYTON
"SERVICE" OPERATIONSCENTER
"ENVIRONMENT"
ELECTRIC SERVICE•
PUBLIC WORKS
[phone]
[phone]
VEHICLE MAINTENANCE •
WATER RECLAMATION
[phone]
[phone]
September 25, 2017
NCDENR/DWR
WQ Permitting Section — NPDES Unit
1617 Mail Service Center
Raleigh, North Carolina 27699-1617
Subject. Renewal of the NPDES Permit NCO025453 RECENEEDINCDEQI®WR
Little Creek Water Reclamation Facility SEP 2 9 2017
Clayton, North Carolina
Johnston County Water
permitting �gectlon
To Whom It May Concern:
Attached Please find three (3) copies, each, of the following items for the subject renewal of
the NPDES Permit No. NCO025453 for the Little Creek Water Reclamation Facility, Clayton,
North Carolina•
• Completed NPDES Form 2A Application for renewal of the NPDES Permit No.
NC0025453, including a Topographic Map and Schematic Process Flow Diagram of the
Clayton LCWRF.
• Toxicity Testing Data in support of the Part E of the application.
Please note that based upon reported data, on April 30, 2015, the Division concurs that the
Clayton LCWRF meets the conditions for exceptional compliance monitoring frequency
reduction which resulted in the Permit modification to provides the following changes, effective
immediately:
• Modify Effluent Sheet A. (1.) from Daily monitoring to 2/week monitoring for BODS, TSS,
NH3-N, and Fecal Coliform. Please see the attached with the April 30, 2015 letter from
653 highway 42 West • PO Box 879 • Clayton, North Carolina 27520 • [phone] • Fax [phone]

I
the Division and the modified Effluent Sheet A. (1.) page for the 2 5 MGD permitted
flow with the reduced monitoring frequency.
It should be noted that during the period March 20, 2017 through June 19, 2017 chlorine and
calcium thiosulfate were used during the UV replacement project . The Clayton LCWRF"s
effluent Total Chlorine Residual concentration during the period was < 20 ug/I, and complied
with the permit limit of 28 ug/I.
Please note that as per the agreements, the Town of Clayton will be discharging up to 1.3 MGD
of wastewater to the Johnston County Wastewater Treatment Plant and up to 1.0 MGD to the
City of Raleigh's Neuse River Wastewater Treatment Plant. The discharge to the City of Raleigh's
Neuse River Wastewater Treatment Plant will occurs after the recently constructed pump
station is placed on line
Should you have any questions during the review of the renewal of the NPDES Permit
application, please contact James Warren at 919-553-1536 or Shankar Mistry at 919-272-4924
Sincerely,
Ada Lindsay
Town Manager, Clayton, NC

September 15, 2017
Point Source Branch
Surface Water Protection Section
Division of Water Resources
1617 Mail Service Center
Raleigh, NC 27699-1617
Subject: Delegation of Signature Authority
Little Creek Water Reclamation Facility
Clayton, North Carolina
NPDES Permit No: NC0025453
To Whom It May Concern:
By notice of this letter, I hereby delegate signatory authority to each of the following
individuals for all permit applications, discharge monitoring reports, and other
information relating the operations at the Little Creek Water Reclamation Facility as
required by all applicable Federal, State, and Local environmental agencies specifically
with the requirements for signatory authority as specified in 15A NCAC 213.0506.
Name Title
Nancy Medlin Deputy Town Manager
James Warren Wastewater Operations Superintendent
If you have any questions regarding this letter, please feel free to contact me at
919-553-5002.
Sincerely,
Adam Lindsay
Town Manager, Clayton, NC
Cc: NCDWR Raleigh Regional Office
1

FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
PERMIT ACTION REQUESTED.
RIVER BASIN:
NCO025453
Renewal
Neuse River Basin
2A IWPDES FORM 2A APPLICATION OVERVIEW
NPDES
APPLICATION OVERVIEW
Form 2A has been developed in a modular format and consists of a "Basic Application Information" packet
and a "Supplemental Application Information" packet. The Basic Application Information packet is divided
into two parts. All applicants must complete Parts A and C. Applicants with a design flow greater than or
equal to 0.1 mgd must also complete Part B. Some applicants must also complete the Supplemental
Application Information packet. The following items explain which parts of Form 2A you must complete.
BASIC APPLICATION INFORMATION:
A. Basic Application Information for all Applicants. All applicants must complete questions A 1 through A 8 A treatment works
that discharges effluent to surface waters of the United States must also answer questions A 9 through A 12
B. Additional Application Information for Applicants with a Design Flow >_ 0.1 mgd. All treatment works that have design flows
greater than or equal to 0 1 million gallons per day must complete questions B 1 through B 6
C. Certification. All applicants must complete Part C (Certification)
SUPPLEMENTAL APPLICATION INFORMATION:
D. Expanded Effluent Testing Data. A treatment works that discharges effluent to surface waters of the United States and meets
one or more of the following criteria must complete Part D (Expanded Effluent Testing Data)
1 Has a design flow rate greater than or equal to 1 mgd,
2 Is required to have a pretreatment program (or has one in place), or
3 Is otherwise required by the permitting authority to provide the information
E. Toxicity Testing Data. A treatment works that meets one or more of the following criteria must complete Part E (Toxicity Testing
Data)
1 Has a design flow rate greater than or equal to 1 mgd,
2 Is required to have a pretreatment program (or has one in place), or
3 Is otherwise required by the permitting authority to submit results of toxicity testing
F. Industrial User Discharges and RCRA/CERCLA Wastes. A treatment works that accepts process wastewater from any
significant industrial users (SIUs) or receives RCRA or CERCLA wastes must complete Part F (Industrial User Discharges
and RCRA/CERCLA Wastes) SIUs are defined as
1 All industrial users subject to Categorical Pretreatment Standards under 40 Code of Federal Regulations (CFR) 403 6 and
40 CFR Chapter I, Subchapter N (see instructions), and
2 Any other industrial user that
a Discharges an average of 25,000 gallons per day or more of process wastewater to the treatment works (with certain
exclusions), or
b Contributes a process wastestream that makes up 5 percent or more of the average dry weather hydraulic or organic
capacity of the treatment plant, or
c Is designated as an SIU by the control authority
G. Combined Sewer Systems. A treatment works that has a combined sewer system must complete Part G (Combined Sewer
Systems)
ALL APPLICANTS MUST_COMPLETE PART C (CERTIFICATION)
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 1 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED-
RIVER BASIN.
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
BASIC APPLICATION INFORMATION
PART A. BASIC APPLICATION INFORMATION FOR ALL APPLICANTS:
All treatment works must complete questions A.1 through A.8 of this Basic Application Information Packet
A.1 Facility Information
Facility Name Little Creek Water Reclamation Facility
Mailing Address Post Office Box 879
Clayton, North Carolina 27520
Contact Person James O Warren
Title Waste Water Operations Superintendent
Telephone Number [phone]
Facility Address 1000 Durham Street Extension
(not P O Box) Clayton North Carolina 27528
A.2. Applicant Information. If the applicant is different from the above, provide the following
Applicant Name Adam Lindsay Town Manager
Mailing Address 111 East Second Street
Clayton North Carolina 27520
Contact Person Adam Lindsay
Title Town Manager
Telephone Number [phone]
Is the applicant the owner or operator (or both) of the treatment works?
® owner ❑ operator
Indicate whether correspondence regarding this permit should be directed to the facility or the applicant
® facility ❑ applicant
A.3. Existing Environmental Permits Provide the permit number of any existing environmental permits that have been issued to the treatment works
(include state -issued permits)
NPDES Permit No NCO025453 PSD N/A
UIC NIA Other W00022224
RCRA NIA Other
A.4. Collection System Information. Provide information on municipalities and areas served by the facility Provide the name and population of each
entity and, if known, provide information on the type of collection system (combined vs separate) and its ownership (municipal, private, etc)
Name Population Served Type of Collection System Ownership
Town of Clayton 19,102 Separate Town of Clayton (Municipal)
East Clanton Industrial Area Industrials Separate Town of Clayton (Municipal)
Total population served 19,102
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 2 of 22

FACILITY NAME AND PERMIT NUMBER:
Little Creels Water Reclamation Facility,
NCO025453
PERMIT ACTION REQUESTED: RIVER BASIN.
Renewal I Neuse River Basin
A.5 Indian Country.
a Is the treatment works located in Indian Country?
❑ Yes ® No
b Does the treatment works discharge to a receiving water that is either in Indian Country or that is upstream from (and eventually flows
through) Indian Country?
❑ Yes © No
A.6 Flow Indicate the design flow rate of the treatment plant (i e , the wastewater flow rate that the plant was built to handle) Also provide the
average daily flow rate and maximum daily flow rate for each of the last three years Each year's data must be based on a 12 -month time period
with the 121 month of "this year" occurring no more than three months pnor to this application submittal
a Design flow rate 2.50 mgd
Two Years Ago Last Year This Year
b Annual average daily flow rate 1.559 1.398 1.515
C Maximum daily flow rate 4 631 3.766 5.82
A 7. Collection System. Indicate the type(s) of collection system(s) used by the treatment plant Check all that apply Also estimate the percent
contribution (by miles) of each
® Separate sanitary sewer 100 %
❑ Combined stone and sanitary sewer %
A.8 Discharges and Other Disposal Methods.
a Does the treatment works discharge effluent to waters of the U S ? ® Yes ❑ No
If yes, list how many of each of the following types of discharge points the treatment works uses
I Discharges of treated effluent
u Discharges of untreated or partially treated effluent
III Combined sewer overflow points
IV Constructed emergency overflows (prior to the headworks)
V Other
b Does the treatment works discharge effluent to basins, ponds, or other surface impoundments
that do not have outlets for discharge to waters of the U S ? ❑ Yes
If yes, provide the following for each surface impoundment
Location
Annual average daily volume discharge to surface impoundment(s)
Is discharge ❑ continuous or ❑ intermittent?
c Does the treatment works land -apply treated wastewater?
If yes, provide the following for each land application site
Location Pine Hollow Golf Course
Number of acres 97 4 (approximately)
1 (Neuse River)
® No
® Yes
mgd
❑ No
Annual average daily volume applied to site 0.025608 mgd
Is land application ❑ continuous or ® intermittent?
d Does the treatment works discharge or transport treated or untreated wastewater to another
treatment works? ® Yes ❑ No
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 3 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
If yes, describe the mean(s) by which the wastewater from the treatment works is discharged or transported to the other treatment works
(e g , tank truck, pipe)
Pipe (From East Clayton Industrial Area)
If transport is by a party other than the applicant, provide
Transporter Name
Mailing Address
Contact Person
Title
Telephone Number ( )
For each treatment works that receives this discharge, provide the follovong
Name Johnston County Wastewater Treatment Facilities
Mailing Address P O Box 2263
Smithfield North Carolina 27577
Contact Person Chandra Farmers P E
Title Public Works Utilities Director
Telephone Number [phone]
If known, provide the NPDES permit number of the treatment works that receives this discharge NCO030716
Provide the average daily flow rate from the treatment works into the receiving facility 0520,181 mgd
e Does the treatment works discharge or dispose of its wastewater in a manner not included
in A 8 through A 8 d above (e g , underground percolation, well injection) ❑ Yes ® No
If yes, provide the following for each disposal method
Description of method (including location and size of site(s) if applicable)
Annual daily volume disposed by this method
Is disposal through this method ❑ continuous or ❑ intermittent?
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 4 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED: RIVER BASIN.
Little Creek Water Reclamation Facility, Renewal Neuse River Basin
NCO025453
WASTEWATER DISCHARGES:
If you answered "Yes" to question A.8.a, complete questions A.9 through A.12 once for each outfall (including bypass points) through
which effluent is discharged. Do not include information on combined sewer overflows in this section. If you answered "No" to question
A 8.a, go to Part B, "Additional Application Information for Applicants with a Design Flow Greater than or Equal to 0.1 mgd."
A.9 Description of Outfall.
a Outfall number
b Location Town of Clayton -Neuse River between SR 1700 and NC Hwy 42
27527
(City or town, if applicable)
(Zip Code)
Johnston
North Carolina
(County)
(State)
35* 39' 50"
78* 25'26"
(Latitude)
(Longitude)
c Distance from shore (if applicable)
ft
d Depth below surface (if applicable) 3
ft
e Average daily flow rate 25
mgd
f Does this ouffall have either an intermittent or a periodic discharge? ❑ Yes
® No (go to A 9 g )
If yes, provide the following information
Number f times per year discharge occurs
Average duration of each discharge
Average flow per discharge
mgd
Months in which discharge occurs
g Is ouffall equipped with a diffuser?
A.10 Description of Receiving Waters.
❑ Yes ® No
a Name of receiving water Neuse River
b Name of watershed (if known) Neuse Sub Basin 03-04-02
United States Soil Conservation Service 14 -digit watershed code (if known)
c Name of State Management/River Basin (if known) Neuse River Basin
United States Geological Survey 8 -digit hydrologic cataloging unit code (if known) 03020201
d Critical low flow of receiving stream (if applicable)
acute cfs chronic cfs
e Total hardness of receiving stream at critical low flow (If applicable)
mg/I of CaCO3
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 6 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
A.11. Description of Treatment
a What level of treatment are provided? Check all that apply
❑ Primary ❑ Secondary
® Advanced ❑ Other Describe
b Indicate the following removal rates (as applicable)
Design BOD5 removal or Design CBOD5 removal 98 %
Design SS removal > 86 %
Design P removal >75 %
Design N removal 85 %
Other %
c What type of disinfection is used for the effluent from this outfall? If disinfection varies by season, please describe
UV Disinfection
If disinfection is by chlorination is dechlorination used for this outfall? ❑ Yes ❑ No
Does the treatment plant have post aeration? ❑ Yes I] No
A.12. Effluent Testing Information. All Applicants that discharge to waters of the US must provide effluent testing data for the following
parameters. Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is
discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data
collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of
40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a
minimum, effluent testing data must be based on at least three samples and must be no more than four and one-half years apart
Outfall number 001
MAXIMUM DAILY VALUE
AVERAGE DAILY VALUE
PARAMETER
Value
Units
Value
Units
Number of Samples
pH (Minimum)
6.83
s u
pH (Maximum)
8.75
s u
Flow Rate
5.82
mgd
1.544
mgd
1096
Temperature (Winter)
23
Deg. C
17.258
Deg. C
302
Temperature (Summer)
28.8
Deg. C
23,8
Deg. C
448
' For pH please report a minimum and a maximum daily value
MAXIMUM DAILY
AVERAGE DAILY DISCHARGE
POLLUTANT
DISCHARGE
ANALYTICAL
MUMDL
Number of
METHOD
Conc.
Units
Conc.
Units
Samples
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
BIOCHEMICAL OXYGEN
BOD5
16.4
m /1
g
2.08
m /1
g
449
SM5210 B-
2001
2 m /1
g
DEMAND (Report one)
CBOD5
NIA
NIA
NIA
NIA
NIA
NIA
NIA
FECAL COLIFORM
6,000
#1100mi
5.76
#1100m1
456
SM9222-D
NIA
TOTAL SUSPENDED SOLIDS (TSS)
28,4
mg/1
1.95
mg /l
449
SM2540 D
2.5 mg/1
END OF PART A.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS -
OF FORM 2A YOU MUST COMPLETE
EPA Form 3510-2A (Rev 1-99) Replaces EPA fors 7550-6 & 7550-22 Page 6 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED.
RIVER BASIN:
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
BASIC APPLICATION INFORMATION
PART B. ADDITIONAL APPLICATION INFORMATION FOR APPLICANTS WITH A DESIGN FLOW GREATER THAN OR
EQUAL TO 0.1 MGD (100,000 gallons per day).
All applicants with a design flow rate >_ 0.1 mgd must answer questions B.1 through B 6. All others go to Part C (Certification).
B.I. Inflow and Infiltration Estimate the average number of gallons per day that flow into the treatment works from inflow and/or infiltration
438,000 gpd
Briefly explain any steps underway or planned to minimize inflow and infiltration
The Town of Clayton has an ongoinq sewer system evaluation and rehabilitation program
B.2. Topographic Map. Attach to this application a topographic map of the area extending at least one mile beyond facility property boundaries This
map must show the outline of the facility and the following information (You may submit more than one map if one map does not show the entire
area )
a The area surrounding the treatment plant, including all unit processes
b The major pipes or other structures through which wastewater enters the treatment works and the pipes or other structures through which
treated wastewater is discharged from the treatment plant Include outfalls from bypass piping, if applicable
c Each well where wastewater from the treatment plant is injected underground
d Wells, springs, other surface water bodies, and drinking water wells that are 1) within % mile of the property boundaries of the treatment
works, and 2) listed in public record or otherwise known to the applicant
e Any areas where the sewage sludge produced by the treatment works is stored, treated, or disposed
f If the treatment works receives waste that is classified as hazardous under the Resource Conservation and Recovery Act (RCRA) by truck, rad,
or special pipe, show on the map where the hazardous waste enters the treatment works and where it is treated, stored, and/or disposed
B 3. Process Flow Diagram or Schematic. Provide a diagram showing the processes of the treatment plant, including all bypass piping and all
backup power sources or redunancy in the system Also provide a water balance showing all treatment units, including disinfection (e g ,
chlorination and dechlonnation) The water balance must show daily average flow rates at influent and discharge points and approximate daily flow
rates between treatment units Include a brief narrative description of the diagram
BA. Operation/Maintenance Performed by Contractor(s).
Are any operational or maintenance aspects (related to wastewater treatment and effluent quality) of the treatment works the responsibility of a
contractors ❑ Yes ® No
If yes, list the name, address, telephone number, and status of each contractor and describe the contractor's responsibilities (attach additional
pages if necessary)
Name
Mailing Address
Telephone Number ( )
Responsibilities of Contractor
B.5. Scheduled improvements and Schedules of Implementation. Provide information on any uncompleted implementation schedule or
uncompleted plans for improvements that will affect the wastewater treatment, effluent quality, or design capacity of the treatment works If the
treatment works has several different implementation schedules or is planning several improvements, submit separate responses to question B 5
for each (If none, go to question B 6 )
a List the outfall number (assigned in question A 9) for each outfall that is covered by this implementation schedule
b Indicate whether the planned improvements or implementation schedule are required by local, State, or Federal agencies
❑ Yes ❑ No
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 7 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN -
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
c If the answer to B 5 b is "Yes," briefly describe, including new maximum daily inflow rate (if applicable)
d Provide dates imposed by any compliance schedule or any actual dates of completion for the implementation steps listed below, as
applicable For improvements planned independently of local, State, or Federal agencies, indicate planned or actual completion dates, as
applicable Indicate dates as accurately as possible
Schedule Actual Completion
Implementation Stage MM/DD/YYYY MM/DD/YYYY
- Begin Construction
- End Construction
- Begin Discharge
- Attain Operational Level
e Have appropriate permits/clearances concerning other Federal/State requirements been obtained? ❑ Yes ❑ No
Describe briefly
B6. EFFLUENT TESTING DATA (GREATER THAN 0.1 MGD ONLY).
Applicants that discharge to waters of the US must provide effluent testing data for the following parameters Provide the indicated
effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include information
on combine sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate
QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136 At a minimum effluent testing data must be
based on at least three pollutant scans and must be no more than four and on -half years old.
Outfall Number 001
MAXIMUM DAILY
AVERAGE DAILY DISCHARGE
POLLUTANT
DISCHARGE
ANALYTICAL
ML/MDL
Number of
METHOD
Conc.
Units
Conc.
Units
Samples
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
AMMONIA (as N)
6.44
mg/1
0.272
mgll
455
EPA350.1
0.04 mgll
CHLORINE (TOTAL
RESIDUAL, TRC) See Note
< 20
ug/I
< 20
ug/l
92
SM4500 Cl G -
2000 (Agues)
0.02 mgll
Below)
DISSOLVED OXYGEN
10.3
mg/I
7.836
mg/I
748
Hach -10360
(LDO)-2011
0.2 mg/I
TOTAL KJELDAHL
6.04
mg/I
1.184
mg/l
162
EPA 351.2
0.2 mg/I
NITROGEN (TKN)
NITRATE PLUS NITRITE
11.24
mg/i
3 173
mg/I
160
EPA 353.2
0.04 mg/l
NITROGEN
OIL and GREASE
5.0
mgll
< 5.0
mgll
3
EPA 1664A
5.0 mg/I
PHOSPHORUS (Total)
6.53
mg/1
1.059
mg/1
171
EPA 365.4
0.04 mg/I
TOTAL DISSOLVED SOLIDS
401
mg/I
244.3
mg/l
3
SM 2540c-97
1.0 mg/I
(TDS)
OTHER
END OF PART B.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
Note It should be noted that during replacement of the existing UV Disinfection System Chlorination and De -chlorination systems were used from March 20,
2017 throuqh June 19, 2017
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 8 of 22

FACILITY NAME AND PERMIT NUMBER
PERMIT ACTION REQUESTED.
RIVER BASIN:
Little Creels Water Reclamation Facility
Renewal
Neuse River Basin
NCO025453
BASIC APPLICATION INFORMATION
PART C. CERTIFICATION
All applicants must complete the Certification Section. Refer to instructions to determine who is an officer for the purposes of this
certification All applicants must complete all applicable sections of Form 2A, as explained in the Application Overview. Indicate below which
parts of Form 2A you have completed and are submitting. By signing this certification statement, applicants confirm that they have reviewed
Form 2A and have completed all sections that apply to the facility for which this application is submitted.
Indicate which parts of Form 2A you have completed and are submitting:
® Basic Application Information packet Supplemental Application Information packet
® Part D (Expanded Effluent Testing Data)
® Part E (Toxicity Testing Biomomtonng Data)
® Part F (Industrial User Discharges and RCRA/CERCLA Wastes)
❑ Part G (Combined Sewer Systems)
ALL APPLICANTS MUST COMPLETE THE FOLLOWING CERTIFICATION.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system
designed to assure that qualified personnel properly gather and evaluate the information submitted Based on my inquiry of the person or persons who
manage the system or those persons directly responsible for gathering the information, the information is, to the best of my knowledge and belief, true,
accurate, and complete I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment
for knowing violations
Name and official title Adam Lindsay. T wn Ma a e
Signature V-X� -0
Telephone number (919) 5 5002
9
Date signed ( ' 2:7 " 1-7
Upon request of the permitting authority, you must submit any other information necessary to assure wastewater treatment practices at the treatment
works or identify appropriate permitting requirements
SEND COMPLETED FORMS TO:
NCDENR/ DWQ
Attn: NPDES Unit
1617 Mail Service Center
Raleigh, North Carolina 27699-1617
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 9 of 22

FACILITY NAME AND PERMIT NUMBER.
Little Creek Water Reclamation Facility,
NCO025453
PERMIT ACTION REQUESTED.
Renewal
RIVER BASIN:
Neuse River Bassin
SUPPLEMENTAL APPLICATION INFORMATION
PART D. EXPANDED EFFLUENT TESTING DATA
Refer to the directions on the cover page to determine whether this section applies to the treatment works
Effluent Testing: 1.0 mgd and Pretreatment Works. If the treatment works has a design flow greater than or equal to 1 0 mgd or it has (or Is required
to have) a pretreatment program, or is otherwise required by the permitting authority to provide the data, then provide effluent testing data for the following
pollutants Provide the Indicated effluent testing information and any other information required by the permitting authority for each outfall through which
effluent Is discharged Do not Include Information on combined sewer overflows In this section All Information reported must be based on data collected
through analyses conducted using 40 CFR Part 136 methods In addition, these data must comply with QA/QC requirements of 40 CFR Part 136 and
other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136 Indicate In the blank rows provided below
any data you may have on pollutants not specifically listed in this form At a minimum, effluent testing data must be based on at least three pollutant
scans and must be no more than four and one-half years old
Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States )
POLLUTANT
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
ANALYTICAL
METHOD
ML/MD
L
Conc.
Units
Mass
Units
Conc.
Units
Mass
Units
Number
of
Samples
METALS (TOTAL RECOVERABLE), CYANIDE, PHENOLS, AND HARDNESS
ANTIMONY
<3
ug/I
<3
ug/I
3
EPA 200 8
3
ARSENIC
<5
ug/1
<5
ug/1
3
EPA 200 8/SM3113B
5
BERYLLIUM
<1
ug/I
<1
ug/I
3
EPA 200 7/EPA
2008
1
CADMIUM
<1
ug/I
<1
ug/I
3
SM3113B-04
1
CHROMIUM
<5
ug/I
<5
ug/I
3
EPA200 7
5
COPPER
<10
ug/I
<10
ugh
3
EPA200 7
10
LEAD
<5
ug/1
<5
ugA
3
SM3113B-04
5
MERCURY
<1
ng/I
<1
ngA
3
EPA1631E
1
NICKEL
<10
ug/I
<10
ug/1
3
EPA200 7
10
SELENIUM
<10
ug/I
<10
ugA
3
SM3113B-04
10
SILVER
<5
ugA
<5
ug/I
3
EPA200 7
5
THALLIUM
<1
ug/I
<1
ug/l
3
EPA200 8
1
ZINC
122
ug/I
10867
ugA
3
EPA200 7
10
CYANIDE
< 0 005
ug/I
< 0 005
ug/I
3
SM450OCNE-97
0 005
TOTAL PHENOLIC
COMPOUNDS
<5
ug/I
<5
ugA
3
4201-78
5
HARDNESS (as CaCO3)
91
mg/I
7233
mg/I
3
SM2340c-97
1
Use this space (or a separate sheet) to provide information on other metals requested by the permit writer
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 10 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED
RIVER BASIN:
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States )
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
POLLUTANT
ANALYTICAL
MUMDL
Number
Conc.
Units
Mass
Units
Conc.
Units
Mass
Units
of
METHOD
Samples
VOLATILE ORGANIC COMPOUNDS
ACROLEIN
< 100
ugfl
< 100
ug/l
3
EPA624
100
ACRYLONITRILE
< 50
ugll
< 50
u9/1
3
EPA524
50
BENZENE
<5
ugll
<5
ug/l
3
EPA624
5
BROMOFORM
<5
ug/I
<5
ug/l
3
EPA624
5
CARBON
<5
ugll
<5
ugll
3
EPA624
5
TETRACHLORIDE
CHLOROBENZENE
<6
ug/I
<5
u9/1
3
EPA624
5
CHLORODIBROMO-
<5
ug/1
<5
u9/1
3
EPA524
5
METHANE
CHLOROETHANE
<10
ug/l
<10
ugll
3
EPA624
10
2-CHLOROETHYLVINYL
<5
ugll
<6
ug/I
3
EPA624
5
ETHER
CHLOROFORM
8.2
ug/i
< 6.06
ug/I
3
EPA624
5
DICHLOROBROMO-
<5
ug/I
<5
u9/1
3
EPA624
5
METHANE
1,1-DICHLOROETHANE
<6
ug/1
<5
ug/I
3
EPA624
5
1,2-DICHLOROETHANE
<6
ug/1
<5
ug/I
3
EPA624
5
TRANS-I,2-DICHLORO-
<5
ug/I
<6
ug/i
3
EPA624
5
ETHYLENE
1,1-DICHLORO-
<5
ug/1
<6
ugli
3
EPA624
5
ETHYLENE
1,2-DICHLOROPROPANE
< 5
ug/1
<5
ug/I
3
EPA624
5
1,3-DICHLORO-
<5
ugfl
<5
ugll
3
EPA624
5
PROPYLENE
ETHYLBENZENE
<6
ug/I
<5
ug/I
3
EPA624
5
METHYL BROMIDE
<10
ug/l
<10
ug/l
3
EPA624
10
METHYL CHLORIDE
<10
ug/l
<10
ugll
3
EPA624
10
METHYLENE CHLORIDE
<10
ug/l
<10
ug/l
3
EPA624
10
1,1,2,2-TETRA-
<5
ug/1
<6
ug/I
3
EPA624
5
CHLOROETHANE
TETRACHLORO-
<5
ugll
<5
ug/1
3
EPA624
5
ETHYLENE
TOLUENE
<5
ug/l
<5
ug/1
3
EPA624
5
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 11 of 22

FACILITY NAME AND PERMIT NUMBER.
Little Creek Water Reclamation Facility,
NCO025453
PERMIT ACTION REQUESTED.
Renewal
RIVER BASIN:
Neuse River Basin
Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States )
POLLUTANT
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
ANALYTICAL
METHOD
MUMDL
Conc.
Units
Mass
Units
Conc.
Units
Mass
Units
Number
of
Samples
TRICHLOROETHANE
.46
ugli
<6
ug/I
3
EPA624
5
1,1,2"
TRICHLOROETHANE
<6
ug/l
<5
ug/1
3
EPA624
5
TRICHLOROETHYLENE
<5
ugll
<5
ugll
3
EPA624
5
VINYL CHLORIDE
<10
ug/l
<10
ug/1
3
EPA624
10
Use this space (or a separate sheet) to provide information on other volatile organic compounds requested by the permit writer
ACID -EXTRACTABLE COMPOUNDS
P -CHLORO -M -CRESOL
< 20
ug/I
< 20
ug/I
3
EPA625
20
2 -CHLOROPHENOL
<10
ug/I
<10
ug/I
3
EPA625
10
2,4-DICHLOROPHENOL
<10
ug/I
'10
ug/I
3
EPA625
10
2,4 -DIMETHYLPHENOL
<10
u9/1
<10
ug/I
3
EPA625
10
4,6-DINITRO-0-CRESOL
< 50
ug/1
< 50
ug/I
3
EPA625
50
2,4-DINITROPHENOL
< 50
ugA
< 50
ug/I
3
EPA625
50
2-NITROPHENOL
<10
ug/I
<10
ug/I
3
EPA625
10
4-NITROPHENOL
< 50
ug/I
< 50
ug/I
3
EPA625
50
PENTACHLOROPHENOL
< 50
ug/I
< 50
ug/I
3
EPA625
50
PHENOL
<10
ug/I
<10
ug/I
3
EPA625
10
2,4,6-
TRICHLOROPHENOL
<10
ug/I
< 10
ug/I
3
EPA625
10
Use this space (or a separate sheet) to provide information on other acid -extractable compounds requested by the permit writer
BASE -NEUTRAL COMPOUNDS
ACENAPHTHENE
<10
ug/I
<10
ug/I
3
EPA625
10
ACENAPHTHYLENE
<10
ug/I
<10
ug/I
3
EPA625
10
ANTHRACENE
<10
ugA
<10
ug/I
3
EPA625
10
BENZIDINE
< 100
ug/I
<100
ug/I
3
EPA625
100
BENZO(A)ANTHRACENE
<10
ug/I
<10
ugA
3
EIA625
10
BENZO(A)PYRENE
<10
ug/L
<10
ug/I
3
EPA625
10
EPA Form 3510-2A (Rev 1-99) Replaces EPA farms 7550-6 & 7550-22 Page 12 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED
RIVER BASIN.
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NC0025453
Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States )
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
POLLUTANTNumber
ANALYTICAL
MUMDL
Us�t
Conc.
Units
Mass
Units
Conc.
Mass
Units
of
METHOD
Samples
3,4 BENZO-
<10
ugA
<10
ug/I
3
EPA625
10
FLUORANTHENE
BENZO(GHI)PERYLENE
<10
ug/1
<10
ug/I
3
EPA625
10
BENZO(K)
<10
ugA
<10
ug/I
3
EPA625
10
FLUORANTHENE
BIS (2-CHLOROETHOXY)
<10
ug/I
<10
ugA
3
EPA625
10
METHANE
BIS (2-CHLOROETHYL)-
<10
ug/I
<10
ugA
3
EPA625
10
ETHER
BIS (2-CHLOROISO-
<10
ug/I
<10
ug/I
3
EPA625
10
PROPYL)ETHER
BIS (2-ETHYLHEXYL)
61 205
ug/I
< 33 735
ug/I
3
EPA625
20
PHTHALATE
4-13ROMOPHENYL
<10
ugA
<10
ugA
3
EPA625
10
PHENYLETHER
BUTYL BENZYL
<10
ug/I
<10
ugA
3
EPA625
10
PHTHALATE
2 -CHLORO-
<10
ugA
<10
ug/I
3
EPA625
10
NAPHTHALENE
4-CHLORPHENYL
<10
ug/l
<10
ug/I
3
EPA625
10
PHENYLETHER
CHRYSENE
<10
ug/I
<10
ug/I
3
EPA625
10
DI -N -BUTYL PHTHALATE
<10
ugA
<10
ug/I
3
EPA625
10
DI-N-OCTYL PHTHALATE
<10
ug/l
<10
ug/I
3
EPA625
10
DIBENZO(A,H)
<10
ug/I
<10
ug/I
3
EPA625
10
ANTHRACENE
1,2 -DICHLOROBENZENE
<5
ugA
<6
ugA
3
EPA625
5
1,3 -DICHLOROBENZENE
<5
ugA
<5
ugA
3
EPA625
5
1,4 -DICHLOROBENZENE
<5
ug/I
<5
ug/1
3
EPA625
5
3,3-DICHLORO-
<10
ugA
<10
ugA
3
EPA625
10
BENZIDINE
DIETHYL PHTHALATE
<10
ug/l
<10
ugA
3
EPA625
10
DIMETHYL PHTHALATE
<10
ugA
<10
ugA
3
EPA625
10
2,4-DINITROTOLUENE
< 10
ug/I
<10
ugA
3
EPA625
10
2,6-DINITROTOLUENE
<10
ug/I
<10
ug/I
3
EPA625
10
1,2 -DIPHENYL-
<10
ugA
<10
ugA
3
EPA625
10
HYDRAZINE
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 13 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States )
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
POLLUTANT
ANALYTICAL
MUMDL
Number
Conc.
Units
Mass
Units
Conc
Units
Mass
Units
of
METHOD
Samples
FLUORANTHENE
<10
ug/l
<10
ug/I
3
EPA625
10
FLUORENE
< 10
ug/I
<10
ug/l
3
EPA625
10
HEXACHLOROBENZENE
<10
ug/i
<10
ug/I
3
EPA625
10
HEXACHLORO-
<10
ug/l
<10
ug/l
3
EPA625
10
BUTADIENE
HEXACHLOROCYCLO-
<10
ug/I
<10
ug/l
3
EPA625
10
PENTADIENE
HEXACHLOROETHANE
<10
ug/1
<10
ug/I
3
EPA625
10
INDENO(1,2,3-CD)
<10
ug/I
<10
ug/l
3
EPA625
10
PYRENE
ISOPHORONE
<10
ugll
<10
ug/I
3
EPA625
10
NAPHTHALENE
<10
ug/I
<10
ug/l
3
EPA625
10
NITROBENZENE
<10
ug/I
<10
ug/I
3
EPA625
10
N-NITROSODI-N-
< 10
ug/i
<10
ug/I
3
EPA625
10
PROPYLAMINE
N-NITROSODI-
<10
ug/l
< 10
ug/l
3
EPA625
10
METHYLAMINE
N-NITROSODI-
<10
ug/I
<10
ugll
3
EPA625
10
PHENYLAMINE
PHENANTHRENE
<10
ug/1
<10
ug/I
3
EPA625
10
PYRENE
<10
ug/I
<10
ug/i
3
EPA625
10
1,2,4-
<10
u9/1
<10
ug/1
3
EPA625
10
TRICHLOROBENZENE
Use this space (or a separate sheet) to provide information on other base -neutral compounds requested by the permit writer
I
�
F
�
I
�
I
I
�
I
-
Use this space (or a separate sheet) to provide information on other pollutants (e g , pesticides) requested by the permit writer
_T_
�=
�
I
I
�
I
�
I
I
END OF PART D.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 14 of 22

FACILITY NAME AND PERMIT NUMBER.
PERMIT ACTION REQUESTED.
RIVER BASIN.
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
SUPPLEMENTAL APPLICATION INFORMATION
PART E. TOXICITY TESTING DATA
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points 1) POTWs with a design flow rate greater than or equal to 1 0 mgd, 2) POTWs with a pretreatment program (or those that are
required to have one under 40 CFR Part 403), or 3) POTWs required by the permitting authority to submit data for these parameters
• At a minimum, these results must include quarterly testing for a 12 -month period within the past 1 year using multiple species (minimum of two
species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results
show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution Do not include
information on combined sewer overflows in this section All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136
• In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years If a whole effluent towcity test
conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation, if one was conducted
• If you have already submitted any of the information requested in Part E, you need not submit it again Rather, provide the information
requested in question E 4 for previously submitted information If EPA methods were not used, report the reasons for using alternate methods
If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E
If no biomomtonng data is required, do not complete Part E Refer to the Application Overview for directions on which other sections of the form to
complete
E.I. Required Tests
Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years
M chronic ❑ acute I Clayton submits quarterly toxicity testing results to NCDENR as required by the NPDES Perms
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years Allow one
column per test (where each species constitutes a test) Copy this page if more than three tests are being reported
Test number Test number Test number
a Test information
Test Species & test method number
Age at initiation of test
Outfall number
Dates sample collected
Date test started
Duration
b Give toxicity test methods followed
Manual title
Edition number and year of publication
Page number(s)
c Give the sample collection methods) used For multiple grab samples, indicate the number of grab samples used
24 -Hour composite
Grab
d Indicate where the sample was taken in relation to disinfection (Check all that apply for each
Before disinfection
After disinfection
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 15 of 22

FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NCO025453
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse River Basin
Test number: Test number: Test number:
e Describe the point in the treatment process at which the sample was collected
Sample was collected
f For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both
Chronic toxicity
Acute toxicity
g Provide the type of test performed
Static
Static -renewal
Flow-through
h Source of dilution water If laboratory water, specify type, if receiving water, specify source
Laboratory water
Receiving water
I Type of dilution water If saltwater, specify "natural' or type of artificial sea salts or brine used
Fresh water
Salt water
t Give the percentage effluent used for all concentrations in the test senes
k Parameters measured during the test (State whether parameter meets test method specifications)
pH
Salinity
Temperature
Ammonia
Dissolved oxygen
I Test Results
Acute
Percent survival in 100%
effluent
LC5o
95% C I
%
%
%
Control percent survival
%
%
%
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 16 of 22

FACILITY NAME AND PERMIT NUMBER
Little Creek Water Reclamation Facility,
NCO025453
PERMIT ACTION REQUESTED-
Renewal
RIVER BASIN-
Neuse River Basin
Chronic
NOEC
%
%
oda
C25
%
Control percent survival
%
%
Other (describe)
m Quality Control/Quality Assurance
Is reference toxicant data available?
Was reference toxicant test within
acceptable bounds?
What date was reference toxicant test
run (MM/DD/YYYY)?
Other (describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ® No If yes, describe
EA. Summary of Submitted Biomonitoring Test Information. If you have submitted biomomtonng test information, or information regarding the
cause of toxicity, within the past four and one-half years, provide the dates the information was submitted to the permitting authority and a summary
of the results
Date submitted / / (MM/DD/YYYY)
Summary of results (see instructions)
The Town of Clayton submits quarterly chronic toxicity tests to NCDENR/DWQ in accordance with their NPDES
Permit Please see attached summary of the most recent test results.
END OF PART E.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 17 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED.
RIVER BASIN
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
SUPPLEMENTAL APPLICATION INFORMATION
PART F. INDUSTRIAL USER DISCHARGES AND RCRA/CERCLA WASTES
All treatment works receiving discharges from significant industrial users or which receive RCRA,CERCLA, or other remedial wastes must
complete part F.
GENERAL INFORMATION:
F.1. Pretreatment program. Does the treatment works have, or is subject to, an approved pretreatment program?
❑x Yes ❑ No
F.2. Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works
a Number of non-categoncal SIUs 2 (Same as CIU)
b Number of CIUs 2 (Same as above)
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 18 of 22

F.3.
Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works Submit additional
pages as necessary
Name Gnfols Therapeutics Inc
Mailing Address 8368 US Highway 70 Business West
Clayton NC 27520 Johnston County
F.4.
Industrial Processes Describe all the industrial processes that affect or contribute to the SI U's discharge
A biological products plant utilizing human blood plasma as its major raw materials in the production of healthcare productions Production steps
include pooling coagulation fractionation dissolving and filtration
F.5
Principal Product(s) and Raw Material(s) Describe all of the principal processes and raw materials that affect or contribute to the SI U's
discharge
Principal product(s) Products include hyper immune fluid replacement proteins following shock antdhermophilic clotting factors and
concentrated antibodies to help combat infectious diseases 0 Citric Acid
Raw matenal(s) 6 -Ammo Caproic Acid Buffer pH 4 01 Calcium Chloride Diatomaceous earth, Glycine, Hydrochloric Acid, Maltose,
Potassium Phosphate Sodium Bicarbonate Sodium Citrate Sodium Phosphate Trihydrate Tryptophan, Acetone. Buffer pH 6 84, CIP 100/CIP
additives Ethanol Guanidine Hydrochloride Isopropyl Alcohol Plasma Human (5% Protein) Sephadex, Sodium Caprylate, Sodium Hydroxide,
Sucrose Urea Ammonium Sulfate Buffer pH 7 0 Citric Acid Glacial Acetic Acid Histidine Lysine Polyethylene Glycol (PEG), Sodium Acetate
Sodium Chloride Sodium Phosphate and Tns Tromethamine -
F.6.
Flow Rate.
a Process wastewater flow rate Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent
517,000 gpd (X) continuous or intermittent)
b Non -process wastewater flow rate Indicate the average daily volume of non -process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent
71,000 gpd (X) continuous or intermittent)
F 7.
Pretreatment Standards. Indicate whether the SIU is subject to the following
a Local limits ® Yes ❑ No
b Categorical pretreatment standards ® Yes ❑ No
If subject to categorical pretreatment standards, which category and subcategory?
Pharmaceutical Manufacturing —
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 19 of 22

FACILITY NAME AND PERMIT NUMBER
Little Creek Water Reclamation Facility,
NCO025453
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN.
Neuse River Basin
F 8. Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e g ,
upsets, interference) at the treatment works in the past three years?
❑ Yes ® No If yes, describe each episode
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE:
F.9. RCRA Waste Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rad or dedicated pipe?
❑ Yes © No (go to F 12)
F 10. Waste transport Method by which RCRA waste is received (check all that apply)
❑ Truck ❑ Rad ❑ Dedicated Pipe
F 11. Waste Description Give EPA hazardous waste number and amount (volume or mass, specify units)
EPA Hazardous Waste Number Amount Units
CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
F12 Remediation Waste Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
❑ Yes (complete F 13 through F 15 ) ® No
F.13 Waste Origm. Descnbe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to ongmate in
the next five years)
F.14 Pollutants List the hazardous constituents that are received (or are expected to be received) Include data on volume and concentration, if
known (Attach additional sheets if necessary )
F.15. Waste Treatment
a Is this waste treated (or will be treated) prior to entering the treatment works?
❑ Yes ❑ No
If yes, describe the treatment (provide information about the removal efficiency)
b Is the discharge (or will the discharge be) continuous or intermittent?
❑ Continuous ❑ Intermittent If intermittent, describe discharge schedule
END OF PART F.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 20 of 22

FACILITY NAME AND PERMIT NUMBER.
PERMIT ACTION REQUESTED:
RIVER BASIN:
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
SUPPLEMENTAL APPLICATION INFORMATION
PART G. COMBINED SEWER SYSTEMS
If the treatment works has a combined sewer system, complete Part G.
G.1. System Map. Provide a map indicating the following (may be included with Basic Application Information)
a All CSO discharge points
b Sensitive use areas potentially affected by CSOs (e g , beaches, drinking water supplies, shellfish beds, sensitive aquatic ecosystems, and
outstanding natural resource waters)
c Waters that support threatened and endangered species potentially affected by CSOs
G.2 System Diagram. Provide a diagram, either in the map provided in G 1 or on a separate dravong, of the combined sewer collection system that
includes the following information
a Location of major sewer trunk lines, both combined and separate sanitary
b Locations of points where separate sanitary sewers feed into the combined sewer system
c Locations of in-line and off-line storage structures
d Locations of flow -regulating devices
e Locations of pump stations
CSO OUTFALLS:
Complete questions G.3 through G 6 once for each CSO discharge Goin
G.3. Description of Outfall.
a Outfall number
b Location
(City or town, if applicable) (Zip Code)
(County) (State)
(Latitude) (Longitude)
c Distance from shore (if applicable) ft
d Depth below surface (if applicable) ft
e Which of the following were monitored during the last year for this CSO?
❑ Rainfall ❑ CSO pollutant concentrations ❑ CSO frequency
❑ CSO flow volume ❑ Receiving water quality
f How many storm events were monitored during the last year?
GA. CSO Events.
a Give the number of CSO events in the last year
events (❑ actual or ❑ approx )
b Give the average duration per CSO event
hours (❑ actual or ❑ approx )
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Paqe 21 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
C Give the average volume per CSO event
million gallons (❑ actual or ❑ approx )
d Give the minimum rainfall that caused a CSO event in the last year
Inches of rainfall
G.S. Description of Receiving Waters.
a Name of receiving water
b Name of watershed/nver/stream system
United State Sod Conservation Service 14 -digit watershed code (d known)
c Name of State Management/River Basin
United States Geological Survey 8 -digit hydrologic cataloging unit code (if known)
G 6. CSO Operations.
Describe any known water quality impacts on the receiving water caused by this CSO (e g , permanent or intermittent beach closings, permanent or
intermittent shell fish bed closings, fish kills, fish advisories, other recreational loss, or violation of any applicable State water quality standard)
END OF PART G.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 22 of 22

Additional information, if provided, will appear on the following pages
FACILITY NAME AND PERMIT NUMBER
PERMIT ACTION REQUESTED:
RIVER BASIN:
Little Creek Water Reclamation Facility,
Renewal
Neuse River Basin
NCO025453
Supply the following information for each SIU If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU.
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works Submit additional pages
as necessary
Name Novo Nordisk Pharmaceutical Industries, Inc
Mailing Address 1612 Powhatan Road
Clayton NC 27527
F 4. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge
Sterile insulin products for human diabetic usage are manufactured at Novo Nordisk Pharmaceutical Industries Inc
F.5. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge ,
Principal product(s) Sterile insulin products, FlexPens, FlexTouch, and Liraglutide
Raw matenal(s) Dry insulin crystals hydrochloric acid zinc chloride sodium hydroxide m -cresol, glycerol, phenol, orotamic sulfate,
sodium phosphate sodium acetate sodium chloride zinc acetate
F.6 Flow Rate.
c Process wastewater flow rate Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent
42.000 gpd (X) continuous or intermittent)
d Non -process wastewater flow rate Indicate the average daily volume of non -process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent
44,320 gpd (X) continuous or intermittent)
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following
a Local limits ® Yes ❑ No
b Categorical pretreatment standards ® Yes ❑ No
If subject to categorical pretreatment standards, which category and subcategory?
Pharmaceutical Manufacturing (439 40)
NPDES FORM 2A Additional Information

FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NCO025453
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse River Basin
F.8. Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e g ,
upsets, interference) at the treatment works in the past three years
❑ Yes ® No If yes, describe each episode
NPDES FORM 2A Additional Information

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LITTLE'CREEK WRF
Little Creek WRF - NCO025453 Facility Location (Not to Scale)
Town of Clayton
Receiving Stream Neuse River Steam Class WS -IV CA
Drainage Basin. Neuse River Basin Sub Basin. 03-04-02
Permited Flow 2 5 MGD HUC` 0320201
State Grid/USGA Quad` E25NW, Clayton Latitude 350 39' 50" Longitude 7B° 25' 26"

Influent
Existing Process Train
— �is Abandoned
❑ D B I Sludge
Drying Beds
F
I
O
Chem Fe
Clengester I I $ Sludge
Chemical Thick Bli
I Stor Area (93 GPM
I
Trickling
Filter
PS
Secondary i
Clarifier O��� I I
Aerobic xs
P S �'� i Digester � �\
(90,000 Gallons) \
Sludgge
Stabilization/
Storage Tank—
(360,000 Gallons)
Sludge
Stabilization/
Storage Tank--�
(360,000 Gallons)
Anaerobic Selector Tank
First Stage Anoxic Tank
,--Ox Basin No 2
EXHIBIT 1
Legend
Wastewater/ Effluent
Sludge
- — - - —
Decant/ Filter Backwash / Bed Underdrainage
--- Scum
.o Flow Balance at
Design Average Daily Flow
Schematic Process Flow Diagram of Clayton LCWRF
Filter Backwash

�- ra
North Carolina De
partment of Environment and Natural Resources
Pat McCrory
Govemor Donald R. van der Vaart
April 30, 2015 Secretary
Mr. R Steven Biggs, Town Manager
Town of Clayton
P.O. Box 879
Clayton, North Carolina. 27520
Subject: Monitoring Reduction Request at 2.5 MGD
NPDES Permit NCO025453
Little Creek Wafer Reclamation Facility
Class IV Facility
Johnston County
Dear Mr. Biggs:
The Division has reviewed your letter request dated October 17, 2014 to reduce monitoring
frequency at the flow of 2.5 MGD. Based on the reported data from January 2012 through
December 2014 and other criteria, the Division concurs that this facility meets the conditions for
exceptional compliance monitoring frequency reduction. S ecificall this
provides for the following changes, effective immediately: Y' penmit modification
e Modify Effluent Sheet A.(1.) from Daily monitoring to 2/Week monitoring for B ODS,
TSS, NI -B n and Fecal Coliform.
Attached is the modified effluent sheet A.(1.) page for your 2.5 MGD permitted flow with the
reduced monitoring frequency incorporated. Please insert this modified copy and discard the
older version.
If any parts, measurement frequencies or sampling requirements contained in this permit
modification ale unacceptable to you, you have the right to an adjudicator hearing
request within thirty (30) days following receipt of this letter, This request must bin the form of
a written petition, conforming to Chapter 150B of the No
and filed
with the Office of Administrative Hem'ings (6714 Mail Service Ce� Ralei'gh, NortGeneral h Carolina
27699-6714). Unless such demand's made, this decision shall be final and binding.
Please note that his permit is not transferable except after notice to the Division: The Division
�y require modification or revocation and reissuance of the permit. This permit does not affect
the legal requirements to obtain other permits which may be required by the Division of Water
Resources or other Federal, State, or local government entities.
IT Mall Service Cenler, Raleigh, NOM Carolina 27699-1617
Phone: [phone] InQel: www.ncdenr goy
M Equal OA -b y IMrmal @Acbw Empbyar—Ma* In part by rccyc(edpaper

ID
If you have any questions or need additional information, please contact Yang Song at [phone] or
email me at [e-mail].
Sincerely,
. Jay Zimme , Director
Division of Water Resources, DENR
cc: NPDES File
Central Files
EPA Region 4 (e -copy)
Raleigh Regional Office / Surface Water protection section
Raleigh Regional Office /Public Water Supply Section (c -copy)
James O. Warren, ORC, Little Creek Water Reclamation Facility (ecopy)

Permit NCO025453
(Footnotes onnextpage)
Page 3 o€11
PART I
A4-) `EFFLUENT LIMITATIONS AND MONITORING REQUIREMENTS
a• During the period beginning on the effective date of the permit and lasting until expiration, the
Permittee is authorized to discharge
up to 25 MGD Of municipal wastewater from outfall 001.
Such discharges shall be limited and monitoredi2b
Y the Permittee
as specified
below:
FLU9. T Y IITA pNS ly
'
ARA
nCTEiTSJitS
M ks
3 ontlely`: d W�eldyXsMeasu�e�nent �''Samp'; % w�s''Se�
Avg�age �v9e
Flow
z K Ma im'um , q`
'4 r@ ue nry� peF,
�Q
N n,
BOD, 5 day (20°P2
2.5 MGD Continuous
Recording
Influent or Effluent
[April 1— October 31]
5.0 mg/L 7.5 mg/L 2/Week
Com site
po
Influent &Effluent
BOD, 5 day (20°C)2
[November 1— March
31]
10.0 mg/L is, mg/L 2JWeek'
Composite
Influent &Effluent
Total Suspended Solids 2
30.0 mg/L 45.0 mg/L z/Week
NH3 as N
Composite
Influent &Effluent
[April 1— October 31]
1.0 mg/L 3.0 mg/L 2/Week
Com poslte
Effluent
NH3 as N
(Nov 1— March 31]
2.0 mg/L 6.0 mg/L 2/Week
Composite
Effluent
Dissolved Oxygen 3
Dally
Grab
Effluent
Dissolved Oxygen
Footnote 1
Grab
Upstream &
Fecal Collform(9eomeMc
Downstream
mean)
2001,100 mL 400/100 mL 2/Week
Grab
Effluent
Fecal Coliform (geometric
mean)
Footnote 1
Grab
Upstream &
Total Residual Chlorine
Downstream
TKN (mg/L)
28 ug/L Daily
Grab
Effluent
NO2-N + N% -N (mg/L)
Monitor & Report k
Wee y
Composite
Effluent
TN (mg/L) s
Monitor & Report Weekly
Composite
Effluent
Total Monthly Flow (MG)
Monitor &Report Weekly
Com Pete
Effluent
Monitor & Report Monthly
Calculated
Effluent
TN Load V
Monitor & Report Monthly
22,832 ib
lb/yr
Calculated
Effluent
Total Phosphorus e
Annually
2.0 mg/L (Quarterly Average)
Calculated
Effluent
Temperature
p (��
Weekly
Composite
Effluent
Temperature (°C)
Dally
Grab
Effluent
Footnote 1
Grab
Upstream &
Conductivity
Downstream
Conductivity
Daly
Grab
Effluent
Footnote 1
Grab
Upstream &
Chronic, Toxicity 9
Downstream
Total Mercuryio
Quarterly
Composite
Effluent
pH
Quarterly
> 6.0 and < 9.0 standard units
Grab
Effluent
Effluent Pollutant ScanEffluent
Daily
Grab
Footnote 11
Footriote 11
Effluent
(Footnotes onnextpage)
Page 3 o€11

�j2
7

4
TOWN OF CLAYTON
"SERVICE» OPERA TI DNS CENTER
ELECTRIC SERVICE a
[phone]
VEIUCLEMARTMNANCE
[phone]
June 28, 2017
Water Sciences Section
Aquatic Toxicity Branch
DWR
1621 Mail Service Center
Raleigh, NC 27699-1623
Re: Toxicity Results: NPDES# NCO025453
To Whom It May Concern:
"ENVIRONMENT"
PUBLIC WORKS
[phone]
WATERRECL4MATION
[phone]
Enclosed please find the Ceriodaphnia Pass/ Fail toxicity and Chronic Fat Head Minnow
results for the Little Creek Water Reclamation Facility.
Please call me directly at 919-553-1536 if you have any questions.
Sincerely,
J es Warren, ORC
Town of Clayton, NC
653 Highway 42 West - P.O. Box 879 - Clayton, North Carolina 27520 - [phone] - Fax [phone]

effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test
NPDES # NC00 25453 Pipe #. 001 County. Johnston
Date:6/21 /2017
Facility: Clayton
Signature of Laboratory Supervisor 1 '
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Test Initiation DatelTime
6/13/2017
4 20 PM
Avg Wt/Surv. Control 0 891 Test Organisms
% Eff. Repl.
1
2
3
4
Cultured In -House
Control I Surviving #
10
10
9
10
% Survival 97 5 Outside Supplier
Onginal #
Wt/original (mg)
10
0.865
10
1.017
10
0 818
10
0 775
Avg Wt (mg) 0 869 Hatch Date 6/12117
0 5 1 Surviving #
10
10
10
10
% Survivall 1000 Hatch Time, 3 00 pm CT
Original #
Wt/onginal (mg)
10
0.866
10
0.996
10
0.743
10
0.754
Avg Wt (mg) 0840
0 Surviving #
10
9
10
10
% Survival 97 5
Original #
Wt/original (mg)
10
0.741
10
0.883
10
0.972
10
0 808
Avg Wt (mg) 0 851
L-. 1 Surviving #
10
10
10
10
% SurvivalF 1000
Original #
Wt/original (mg)
10
0 906
10
0 977
10
0846
10
0.982
Avg Wt (mg) 0 928
Surviving #
10
10
9
% Survival 97.5
Original #
Wt/original (mg)
10
0.886
10
1.046
10
0.882
Llij
Avg Wt (mg) 0 930
® Surviving #
9
9
10
9
% Survival 92 5
Original #
Wtloriginal (mg)
10
0.937
10
1 016
10
0.990
10
0 977
Avg Wt (mg)F__0__98_0__1
Water Quality Data
Day
Control
0
1
2
3
4 5 6
pH (SU) Init/Fin
8.16 / 7.91
8.18 / 7.81
821 / 7.96
8.16 / 7.95
I 8 23 J 8 08 18.25 1 7.74 819 1 7.64
DO (mg/L) Init/Fin
7.97 / 7.20
124.6
7.84 / 648
7.78 1 7.37
7.88 / 6,881765
/ 7.60 7.85 15 93 1 7.63 / 5 56
Temp (C) Init/Fin
247 / 24.1
/ 24.21249
/ 243124.6
/ 245124.4
/ 24.7 24.2 / 246124.6 / 24.8
High Concentration
0
1
2
3
4 5 6
pH (SU) Init/Fin
8.09 / 792
8.15 / 7.74
8.13 / 7 94
1 813 1 8.05
I 8 19 18.16 8.23 17.73 8.18 1 7.81
DO (mg/L) Init/Fin
7.80 / 7.11
7.77 / 63617.72
/ 7.31
17.53 / 7.07
17.62 / 7.68 7.83 / 602 7.63 / 6.20
Temp (C) Init/Fin
249 / 24.21245
/ 24.71245
/ 24.5
24.2 / 2451246
/ 24.01248 / 24.01245 / 24 2
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mglL)
Alkalinity (mglL)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt (°C)
1 2 3
6/12/2017
6/14/2017
6/15/2017
Normal
1171
F-1
23.8
22.3
248
80
75
76
98
98
90
602
620
540
<0.1
<0.1
<0.1
2.3
26
1.7
Dilution H2O Batch # 1170 1171 1172 1173
Hardness (mg/L) 42 42 42 46
Alkalinity (mg/L) 53 56 54 54
.onductivity (umhos/cm) 189 212 209 198
Overall Result
ChV >8
Stats
Survival
Growth
Normal
1171
F-1
Hom. Var
10 20
T71
NOEC
8
8
LOEC
>8
>8
ChV
>8
>8
Method
Steel's
Dunnett's
Overall Result
ChV >8
Stats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
05
10 20
241 0.4671
1
10 18
2.41 02859
2
10 20
2.41 -0.9502
4
10 18
2.41 -09905
8
10 14
2.41 -1.7917

Client: Clayton
NPDES #: 0025453
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber: 10
Test Vessel Size: 400 ml
Initiated by: i PJB✓ Test Solution Volume:: 250 ml
Daterrime Fed: 6/13/17 lZ 3ap Temp. of Stock: 2 `-7 °C
Daterrime Born: 6/12/17 3;00 PM cr
Organism Source: Aquatox, Inc.
Transferred by:
# of Reps: 4
Analyst(s): MR, CD, LV, SB
Start Date: 6/13/17 Time, 1) 0
End Date. 6/20/17 Time:
Randomization: G
No
Incubator #:
Fed by:
Date
Time Initials
Day 16/
14
( l -V
Day 2
6 / 15
1L�1✓ i�
'3Nfu
Day
6/16
11'Q1 kv
Day 4
6/ 17
`a3 513
Day 5
6/ 18���
30A�
Day 6
—.E/- 19
, . yl—'�—
# of Reps: 4
Analyst(s): MR, CD, LV, SB
Start Date: 6/13/17 Time, 1) 0
End Date. 6/20/17 Time:
Randomization: G
No
Incubator #:
Fed by:
Day 6 6/19 ,1 1'" LV 3 (CC) 0 LAO
Test Termination Data: /
Initials/Signature:�•v ��Q,.IciV�+'�onf/r�'1
End Date: 6/20/17
Date
Time 1
Initials
Time 2 Initials
Day 0
6/13
. 3 0 JI
,/% G�
(c)
Day 1
6/14
('y
L1/
`a3 513
Day
6/15
30A�
pop
Day 3
6/1.6
F 07am
��
/
- 27 jib/
Day 4
6/17
Ot-10A-^cL
W
Day 5
6/18
.tai r1
(�✓
• � Cio
Day 6 6/19 ,1 1'" LV 3 (CC) 0 LAO
Test Termination Data: /
Initials/Signature:�•v ��Q,.IciV�+'�onf/r�'1
End Date: 6/20/17

11ME RI THCHo, lI v4'_
- .- ENVIRONMENTAL LABORATORIES
= q o;vision of Wafer Technology and Contro/s Inc_
Chemical and Physical Determinations
Client: Clayton NPDES#: 0025453 StartDate: 6/13/17
Test Organism: Pimen hates promelas End Date: 6/20/17
Concentration:
1.00%
Concentration: control
0-1
1-2
2-3
Soft Synthetic Freshwate
Batch #
17
i
PH: Initial
, i
-
Initial
Final
G(i
�1
;04"
D.O.: Initial
�- ?
0 5
2L'S
I
Final
7
11 __
�t K
Temp.: Initial
Final
iz,�,;
Conductivity: Initial
Gcn.l
moi?
Final
�1��
7t
22
Residual Chlorine:
c
z v 1
GUS 1
Hardness:
L4
Alkalinity:
�3
(0
Concentration:
1.00%
Concentration: 0.50%0-1
PH:
1-2
2-2
PH: Initial
. l3
1
Final
-
Initial
D.O.: Initial
r
;04"
Final
Temp.: Initial
(
0 5
2L'S
I
Final
ZIC',
Conductivity: Initial
Final
iz,�,;
Residual Chlorine:
Gcn.l
moi?
Concentration:
1.00%
0-1
PH:
Initial
7
D.O.:
Final
Initial
Final
Temp.:
Initial
'o .
Final
;04"
Conductivity:
Initial
(
Final
Residual Chlorine:
ZIC',
1-2 1 2-3
3-4 4-5 5-6 6-7 Remarks
it $I�
T -7i %'© GW
7 rt�
�.' 4
Lott
GOtt 16
MAM
mom
�ED
®M
��M
M®

- , MER/TECH /NC_
ENVIRONMENTAL LABORATORIES -
4 O/v/s/on c� W rem T Chr.o/o BY +^d Co^rro/s. /^
Chemical and Physical Determinations
Page 2 of 2
Client: Cla on NPDES#: 0025453 Start Date- 6/13/17
Test Organism: Pimepha/es promelas End Date: 6/20/17
Da
Concentration:
2.0%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH:
Initial
�'
-T
(31
�9
[o
-
�'�s5n)
CA
Initial
-Z
Final
CL �-
7r 8 s
! ! 5
D.O.:
Final
', (;
D.O.:
Initial
4
7r
,11
Temp.:
% 7
PLI .
Temp.:
Final
Initial
Final
;, - o
.z
1.29
Conductivity:
t4
it
6L) 1 S
Conductivity:
Initial
/
n �-�
Final
_ �}',�
j�
off-
$ 3
R(b
Final
!� �i
` �3
Z,
�c,l
°'['
!�� �p
Residual Chlorine-
/—c) -Ll
.
rz/-C-"L
I
Ltn 1
Da
Concentration:
4%
0-1
1-2
2-3
3-4
4-5
5-6
6-7 Remarks
pH:
Initial
Final
`,h
�'
-T
(31
ti
[o
-
C�
D.O.:
Initial
-Z
Sj i d
7r 8 s
D.O.:
Final
', (;
-li-M
o
f
7r
,11
Temp.:
Initial
Final
PLI .
94
74f-/
��
a �[-
�-L1 I
iO.J-4-
Conductivity:
Initial
S-
'��
6L) 1 S
rRaQ,
Q
-7 6
�l
Final
_ �}',�
j�
off-
$ 3
R(b
109,
2 4,-A
Residual Chlorine:
Z,
�c,l
�-
��L
Gvi
T)av
SG (6-1s-17
Concentration:
8.0%
0-1
1-2
,2-3
34
4-5
5-6
6-7 Remarks
pH:
Initial
R7%
i
211
VV -
—AY1
Final
Sj i d
q1
D.O.:
Initial
7, a
-li-M
o
,11
Final
-it G
I -Q
Temp.:
Initial
a4.
�l
Final
. a..
;�H.7
QU.5
T%4/
V. b
L1,0
2 4,-A
Conductivity:
Initial
oZa
�-
236
Final
'N`�
la
o�8
Residual Chlorine:
0
LCA
,
u)
Z -C
Day
Concentration: 100% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: 22 Lt
Conductivity: to
Residual Chlorine: k -11 �
Hardness:
Alkalinity: 6 5? qg --_- - n- -- - _

I
1 Client: Clayton
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Test Organism: Pimephales promelas
Concentration Control
N PDES#: 0025453
Start Date: 6/13/17
End Date: 6/20/17
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 0 0
Rep B 0 f /_ CL
Rep C 0
Rep D 0 (0 r v
i
Concentration 0.550%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 CD
� O
Rep B 0 _ V
Rep C 0
1
� Rep D 0 0 0
Concentration 1.00%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
u
Rep A 0 D G -
Rep
� B 0 (� L
Rep C 0 V v
Rep D 0 O r
1

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client Clayton NPDES#: 0025453 Start Date: 6/13/17
Test Organism: Pimephales vomelas End Date: 6/20/17
Concentration 2.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Concentration 4%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
—
Rep A
tel -
Rep B 0
Rep C 0
P — --T-- --— —— l Fv
Re D 0
Concentration 8.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
�� --
Rep B 0 `'
Rep C 0 V
Rep D 0�--
—�—
Rep A 0
Rep B 0
O
Rep C 0—
RepD- 0�—
/f
Concentration 4%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
—
Rep A
tel -
Rep B 0
Rep C 0
P — --T-- --— —— l Fv
Re D 0
Concentration 8.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
�� --
Rep B 0 `'
Rep C 0 V
Rep D 0�--

Facility: Clayton
NPDES #: 0025453
ENVIRONMENTAL LABORATORIES
A O.visicn of Mater Techno%gy and Controls /nc
FISH LARVAL SURVIVAUGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Date of Test: 6/13/17
Initial weights taken on: i00 by:
Final weights taken on: 6v -,,,2 1-1-7 by: Ll-'
Page: 1 of 2
Outfall:
Organism: Pimephales promelas
CONC
REP
PAN WT.
(mg)
PAN t ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV %
A
D6, 3 6
B
93- 0, O
l? 0,1 [
bb/7
106
L
O
C-'
c i qci
\J(' �/jam, '"'/]
(J�' Jf �(
�
jf
q0U
D�
77;
10
o.-7-/5
100
A
V F i �J
�
�- 66
(0+ -'9C `v
/00
B
�� �.y
q. q 6
/0
O. �G q 6
100
0.50%
C
as ,,S
��19 e (9
7` L-1
D
I Lj cSl,Sv
� �'�
7 5q
100
A
g?
—7,I
��
Oa�ql
100
B
57
1.00%
i s
9.�y. 6 7
q, 79
'/0
0, q %Q
IX
D
E55. '/ q
16
U F F6
���

E ®TECHj INC..
>0`0_
ENVIRONMENTAL LABORATORIES
A p,vls,On Of Water Technology and Con r`O'5 /
FISH LARVAL SURVIVALIGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Clayton
NPDES #: 0025453
Date of Test: 6/13/17 C
Initial weights taken on: I ��" bV:_ &P5 -
Final weights taken on: by: LV
Page: 2 of 2
Outfall:
Organism: Pimephales promelas
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV %
CONC
2.0%
A
i �l
- 1�
677
��a• a�
77
L11
0
10.
1
�� F � � �
6AT7
D
too
100
—B
D
n��}. �t
�r
to
Q. u
(QQ
4%
A.
�� ��
ELE(dL
rsi
nq, o -3((s
F
/D. q6
W,Wq
Q 7
10
to
-1
1. oq6
o.wa
0• q6_7
Ino
9b—
ICO
B
c
8.0%
6 437
qO
B
qq. (q
C
r
10_79.
q,77
6477
�O

Sample ID
MERITECH, INC. Meritech • `� `�
Bioassay sample Chain of custody
642 Tamco Rd. Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech4abs.com
CLIENT INFORMATION
Client j DG%�
0-r" C� 6�'i 7 i��
PtJtf -
�.
NPDES#: NC 0D.ZJ'3 s.3
Contact Person:
Address:
Pa J60,x-
U 9
Phone:
City:
YT7li-7
Pipe #:
County -
��� .� tv�
J
Time: r y�
State: elJ� Zp:1�Z
PM
Relinquished by:
Date: , -1✓7 "%%
SAMPLE INFORMATION
Sample Site:
/ �y-/i
/Q'P(
/
j^eek 4
Aft //� �Glgj� 1<io�✓ �L�G! l•f`� -
Sample Type:
❑ Grab
Composite
# of containers:✓ _
Sampling Time: Start Date: ��l1 „Z,%1'% Start Time. S PM
End Date: ���/J�� �% End Time: : !'O PM
*** Triple rinse sample container with sample before filling. Completely fill the sample container with no airspace.
Pack the sample cooler completely in ice. The sample must be < 6-OOC upon receipt
at the laboratory'
Collector's Name: Print%/t4 /� �� �"lSignature.
TOXICITY TEST INFORMATION
Test Required: %Chronic (7 days) Test Organism: MCedodaphnia dubia (water flea)
❑ Acute (24-48 hours) jg�Pimephales promelas (fathead minnow)
Relinquished by:
Received b)r
Date: ! rime: —6' -AM OP
Sample Condition:
Sample Temperatures (°C):
❑ Mysidopsis bahia (shrimp)
lwc: %
Test Concentrations (if multiple dilutions).
Comments:
SHIPPING INFORMATION .y
Date:
J
Time: r y�
AM
PM
Relinquished by:
Date: , -1✓7 "%%
Time: j j. �G
AM
PM
Received by.
by:
Date
Time: V
AM
PM
Relinquished
Time:
AM
PM
Received byA' :
Date.
AM
PM
Relinquished by.
Date:
Time:
Received by:
Date:
Time-
AM
PM
Relinquished by.
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Sample Temperature CC):
Method of Shipment ❑ UPS ❑ Fed EX x Meritech Pick-up ❑ Delivered
❑ Other
.
be FedEx and must be dearly labeled for Saturday delivery. NO SIGNATURE REQUIRED
..Samples shipped on Friday must
0 , �„
SAMPLE RECEIVING (Laboratory Use Only}
Relinquished by:
Received b)r
Date: ! rime: —6' -AM OP
Sample Condition:
Sample Temperatures (°C):

MERITECH, INC- Mentech Sample ID M 06
Bloassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike reed(d0eritech-labs-com _ Web Site: www.mentech-labs-com
// CLIENT INFORMATION
�'"
Client �f�i J bf� 01 Q zhgx� P
NPDES#.
Contact Person: .r +w'
Phone:
Address: 9%7-,�.��•' /S�f!+
city.Pipe #:
County I -L State- A&, Zip:
Sample Site: L• rh- C re G L on, f -Ll
Sample Type: ❑ Grab [Acola-11-1-1,7
mposite /y
Sampling Time: Start Date: la -11- /
End Date: & -13-
_ 1
# of containers: 4;7—
Start Time:�%5_
End Time.000_
AM PM
AM PM
*** Triple rinse sample container with sample before filling. Completely rill the sample container with no air space.
Pack the sample cooler completely in Ice. The sample must be < 6.0°C upon receipt at the laboratory'
Collector's Name: Print?• /j.C?,M 5 �` ru 1�Sd.� Signature:`
TOXICITY TEST INFORMATION
Test Required: R[Chronic (7 days) Test Organism: D§Ceriodaphnia dubia (water flea)
1
❑ Acute (2448 hours) Pimephales promelas (fathead minnow)
0 Mysidopsis bahia (shrimp)
IWC: v % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
Date: f (
Time: Dc�� AM �PM
Relinquished b
Date: 1011- s�/ %
Time: /OZ O S
AM
Received by
�G^
Date: 611
Time: / S3 o
AM
PM
Relinquished by:
Received by:
Date:
Time:
AM
PM
Relinquished by
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Relinquished by
Date:
Time:
AM
PM
Received by
Date:
Time:
AM
PM
Sample Temperature (°C):
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑ Delivered ❑ Other
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLERECEMNG(Laboratory Use Ont
Relinquished b .
Receive Date: Time: -�-� AMPMI
Sample Temperatures (°C) t / . 1 / Sample Condition: 'T

MERITECH, INC. Meritechsample ID#:
Bloassay SamPle Chain of Custody
- 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
ToAcity Supervisor email: mike [e-mail] Web Site: www.meritech4abs.com
/� CLIENT INFORMATION
Client: �,�.�J 19 130#1
Contact Person:„2Q /'/'L / NPDES#: NCO0;25'Y :
q `.fig Phone:2%9'3.x.3
City: Pipe #:
County:TDG�,�.�i�✓ State: zip:
SAMPLE INFORMATION
rff.
Sample Site: L 1#,e,
Sample Type: ❑ Grab Composite # of containers:_
Sampling Time: Start Date: �,� �1J —/ % Start Time: /0&V AM PM
End Date: End Time: cam; PM
*** Triple rinse sample container with sample before filling. Completely rill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory'
Collector's Name: Print i �f Qc,�L �. $;,4/aSa Signature:
,r
TOXICITY TEST INFORMATION
Test Required: Chronic (7 days) Test Organism: XCeriodaphnia dubia (water flea)
❑ Acute (2448 hours) It4Pimephales promelas (fathead minnow)
❑ Mysidopsis Bahia (shrimp)
IWC: r �% Test Concentrations (if multiple dilutions):
Comments-
�f �% SHIPPING INFORMATION
Relinquished by. % G ,tl� Date: %%d i7 Time: O M PM
Received by:
Date: 1/ (O ` 7 Time: AM PM
Relinquished by l Date: ��� 6117 Time: /yya AM �M
Received by: Date: Time. AM PM
Relinquished by:
Received by.
Relinquished by:
Received by.
Date:
Date:
Date:
Date:
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Sample Temperature (°C):
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑ Delivered ❑ Other
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLE RECENING Laborato Use Onl
Relinquished by:
Received by. Date: Time: f r -t -i AM
Sample Temperatures (°C):// / Sample Condition: ��

itle: Clayton Transform: NO TRANSFORMATION
ile-. ClaytonGrow
Shapiro - Wilk's Test for Normality -------------------
D = 0.1388
W = 0.9357
f
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
--------------------------------
Continue analysis.
-------------------------------------------�
I
3ata PASS normality test (alpha = 0.01).
{
I

itle: ClaytonTransform: NO TRANSFORMATION
'ile: ClaytonGrow
Bartlett's Test for Homogeneity of Variance -
-----------------------------
:alculated B1 statistic = 4.2706 (p -value = 0.5111)
)ata PASS B1 homogeneity test at 0.01 level. Continue analysis.
�ritical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)

itle: Clayton Transform: NO TRANSFORMATION
ile: ClaytonGrow
ANOVA Table
----------------------- ---------------------------------
SOURCE DF SS MS
------------------------
----------------------
Between50.0604 0.0121 1.5658
Within (Error) 18 0.1388 0.0077
--------------------------------------------
Total 23 0.1992
----------------------------------
-----------------------(p-Value = 0.2199)
Critical F = 4.2479 (alpha = 0.01, df = 5,18)
= 2.7729 (alpha = 0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

itle: Clayton Transform: NO TRANSFORMATION
ile• ClaytonGrow
Ho:Control<Treatment
Test - TABLE 1 OF 2
____
-----Dunnett's
- ----------
- - - - - - - - -------------------------
TRANSFORMED
MEAN CALCULATED I
SIG
0_05
}ROUP
IDENTIFICATION
MEAN
-- ORIGINAL - UNITS
--
T -STAT
_ 1-- --------------------
Control
-----------
0.8688
0-.8688
0.8398
0.4671
2
050
.
0.8398
0.8510
0.2859
3
1.00
0.8510
0.9277
-0.9502
4
2.0
4
0.9277
0.9303
0.9303
-0.9905
i
8.0
0.9800
6
�--------------------------------
:)unnett
1
critical value = 2.4100
-----------------
(1 Tailed,
alpha = 0.05, df =
5,18)
Title:
Clayton
Transform: NO TRANSFORMATION
E File:
ClaytonGrow
i
j Dunnett's Test -
TABLE 2 OF 2
Ho:Control<Treatment
' j ---------------'--
-------------------
----------------
NUM OF MIN SIG DIFF o OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS (IN ORIG. UNITS) CONTROL
--------------------
FROM CONTROL
1
Control
4
4
0.1496 17.2
0.0290
2
0.50
4
0.1496 17.2
0.0177
30.1496
1.00
17.2
-0.0590
4
24
4
0.1496 17.2
-0.0615
I 6
8.0
4
0.1496 17.2
-0.1112
'------------------------
-------
-----

itle: Clayton Transform: NO TRANSFORMATION
ile: ClaytonSury
Shapiro - Wilk's Test for Normality ------------
D = 0.0300
W = 0.8544
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
-------------------------------------------------------------------------
i
Data FAIL normality test (alpha = 0.01). Try another transformation.
I, yarning - The first three homogeneity tests are sensitive to non -normality
i and should not be performed with this data as is.
I

Ltle: Clayton Transform: NO TRANSFORMATION
ile: ClaytonSury
artley's Test for Homogeneity of Variance
artlett's Testfor Homogeneity of Variance---------------------------------
-
'hese two tests can not be performed because at least one group has
.ero variance.
)ata FAIL to meet homogeneity of variance assumption.
additional transformations are useless.
-------------------------------

Ltle: Clayton Transform:
1ClaytonSury
NO TRANSFORMATION
L e.
Control<Treatment
Steel's Man One
Rank Test -
Ho:
-----------------
---------------------
--------
MEAN IN
RANK
CRIT.
SIG
0_05
ROUP
IDENTIFICATION
ORIGINAL UNITS
SUM
VALUE
-DF--
----------
Control
0.9750
1.0000
20.00
10.00
4.00
2
2
0.50
1.00
0.9750
18.00
10.00
4.00
2.0
1.0000
20.00
10.00
4.00
4
4
0.9750
18.00
10.00
4.00
5
58.0
0.9250
14.00
10.00
4.00
:ritical
values are 1 tailed
( k = 5 )

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 06/21/17
Facility: CLAYTON WWTP NPDES#: NCO025453 Pipe#: 001 County: UGHNSTON
Laboratory Performing Test: MERITECH LABS, INC.
X ���
Comments:
X
Signature ot Laboratory"PASSED: -1
Supervisor * .969.- Reduction
Work Order: Environmental Sciences Branch
MAIL ORIGINAL TO: Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Nnrth ('arnl i na ('pori nA�rl�ni a
Chronic Pass/Fail Reproduction Toxicity Test
:ONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced 1122121119124121119118119124127118123
Adult (L)ive (D)ead 11L IL IL IL IL IL IL IL IL IL IL IL
affluent .-: 2.0.-
CREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced 1127119123121126118122120124121119120
Adult (L)ive Mead IIL IL IL IL IL IL IL IL IL IL IL IL
Chronic Test Results
Calculated t = -0.360
Tabular t = 2.508
.- Reduction = -1.96
t Mortality
Avg.Reprod.
0.00
21.25
Control
Control
0.00
21.67
Treatment 2
Treatment 2
Control CV
13.3290 PASS FAIL
t control orgs X
producing 3rd
brood Check One
100. -
1st sample 1st sample 2nd sample Complete This For Either Test
PH Test Start Date: 06/14/17
Control 7.99 8.14 7.98 8.03 8.02 8.08 Collection (Start) Date
Sample 1: 06/12/17 Sample 2: 06/14/17
Treatment 2 8.02 8.07 7.94 7.96 8.05 8.03 Sample Type/Duration 2nd
s s s Grab Comp. Duration D 1st P/F
t e t e t e I S S
a n a n a n Sample 1 X 23.8 hrs L A A
r d r d r d U M M
t t t Sample 2 X 22.3 hrs T P p
1st sample 1st sample 2nd sample
D.O. Hardness (mg/1) 47
Control 7.76 7.63 7.70 7.40 7.82 7.56
Spec. Cond.(pmhos) 160 602 620
Treatment 2 7.84 7.59 7.68 7.34 7.75 7.53
Chlorine (mg/1) <0.1 <0.1
LC50/Acute Toxicity Test Sample temp. at receipt(OC) 2.3 2.6
(Mortality expressed as .-, combining replicates)
.-
.-
.-
.-
.-
.-
.-
.-.-
Concentration
Mortality-
start/end
LC50 = .- Method of Determination
95% Con idence Limits Moving Average Probit
%- -- % Spearman Karber - Other
Note: Please
Complete This
Section Also
start/end
pH
Organism Tested: Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT -1 (3/87) rev. 11/95 (DUBIA ver. 4.41)
Control
High
re%" n
pH
Organism Tested: Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT -1 (3/87) rev. 11/95 (DUBIA ver. 4.41)

Meritech, Inc.
Mini Chronic Pass/Fall Test Cenodaphnia dubta / Incubator #.
Client*_ C� i/ �n Pipe #: nol County. j Date Start: to �� `� Date End: (:,Z1
NPDES #: MC Date Date / Time of Culture Transfer ; 37 Time Start: % �L? Time End: �o= �/Y1
Dilution Water. Lake Brandt Date /Time Neonates born: I� "• M� �,' ! �,� 1 st Renewal Date: - j (o' �] Time: /� ; am
Test Organism Source: Tray # Age of e:�
onates at Test Start.D hours 2nd Renewal Date. �� �- /'% Time: J© , /IA,6n
Stirred / Aerated for D.O.: Y / N Randomized.UY/ N Culture Tray Temp: 2.HL°C Analyst(s): MR, CD, t.v, ss Reviewed by: �✓'^
Cont_rol_Oroanism Renroduction r.,ne,.+1f%n 1Q+-4% n�.e_.
Dav #2 1 2 A d 5 F 7 A a •I n 1 1 17
#
Young ProducedL
M®mm©
©20al4
0
0
0
0
0
0
0
0
0
0
Adults Live / Dead
tl:
t
(mg1L)
L,
L'_
IL
L-
I L- IL,
Il..
L.
L.
Dav #5 1. 2 3 de .9; . R 7, A a in_ 11 11)
Young Produced
M®mm©
©20al4
Duration
Sam le 1
0 CJ
0
MMmM©m
Sample 2
L�
Adults Live / Dead
�, 3 hours
Adults Live I Dead
WMWWMMMMM
yk
t
(mg1L)
L.
L
fi
Dav #7 1 2 � 4 5 F 7 R a 1 n 1 1 1 7
• • ` • • - •
M®mm©
G / C?
Duration
Sam le 1
0 CJ
0
MMmM©m
Sample 2
L�
Adults Live / Dead
�, 3 hours
Hardness
(�
yk
t
(mg1L)
L.
L
fi
Spec. Cond.
�V
Total Produced
Percent of Control producing third brood.© %
Test Sample Organism Reproduction
Effluent %t
Day #2 1 2 3 4 5 6 7 8
Sample 1: � —ka--� I Sample 2:
Sample Information
Batch #
100% H
G / C?
Duration
Sam le 1
0 CJ
0
hours
Sample 2
L�
Adults Live / Dead
�, 3 hours
Batch #
�a ` ,
Sample 1
Sample 2
Transfer Day
0 CJ
0
0
0
AI�
Adults Live / Dead
(�
Hardness
(�
yk
t
(mg1L)
L.
L
fi
Spec. Cond.
�V
(umhoslcm)
Chlorine
��" ''''
RD Win
:4 i
(mg/L)
Receipt Sample%"F;
Temp ('C)
*4°
9 10 11 12 H 1st Sample
0 0 0 0 Control �Iql
Sample iu� ofi� 91 10 11 12 .a ni
# Young Produced
0
0
0
0
0
0
0
0
AI�
Adults Live / Dead
(�
L
(�
I--
L
L
L.
L
uay #5 1 . 2 3. 4 5 . B. 7, R
• • Produced
VINk7�
a
1—Mg
D
rlWAAIVi7Om
AI�
©�P1J
=M=MMMMMMMnMMMM
L,
lr
L
D.O. 1st Sample
Aav #7 1 2 3 4 5 6 7 8 9 10 11 12 Control
# Young Produced
13,
a
Sample
D
Adults Live / Dead
L,
lr
L
Sample
ini is ma
Temn. 1cr Samn1P
Total Produced TED
T Comments:
Control
,_�
a
Sample
2112
2nd Sample
0
uw i
2nd Sample
n ie ine
2nd Sample
�y y
Transferred by Fed t;
Day 0 /44— LV
Day 1
Day 2 M . LV
Day 3
Day 4 6
Day 5,^ t— v
Day 6
Terminated b
2nd Sample
m ie n�✓"l
2nd Sample
nrer flnaT
2nd Sample

MERITECH, INC. Meritech Sample ID #: V
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supendsor email: mike.reed(Mmeritech-labs.com Web Site: www.meritech-labs.com
/� U CLIENT INFORMATION
Client: �G.J� � � C. �n.� 7 f�,cJ PO#:
Contact Person: JGi~i,eS b,2-;: NPDES#: _ NC
Address: Pa 6pl- r7 Phone:
City: _C /�. f'1Zi,J Pipe #.
County:, OLi rO.�, State:.Zip:
/�
SAMPLE INFORMATION
Sample Site: 1-1,771-4- -L� e-enk r! A -!-!- l/«Gl4w+� ��"c,.✓ �Gt�i 1. fY
Sample Type: ❑ Grab ;4_10mposite # of containers:
Sampling Time. Start Date: _0611,7—JI-7 Start Time: //,-/S- A� PM
End Date: 69 1/V/ I End Time: 11.1067 AM PM
'** Triple rinse sample container with sample before rifling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < &O*C upon receipt at the laboratory'
Collector's Name: Print �� ��« 2 f«,.t/✓d,�/ Signature:
TOXICITY TEST INFORMATION
Test Required: %Chronic (7 days) Test Organism: t4�Cerlodaphnia dubia (water flea)
❑ Acute (24-48 hours) 2-i�Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
lwc: % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION ��ff
Relinquished by- Date: /a "%3 -% 7 Time: 7� AM PM
Received by: Date: t -/3 --
Time: , ` 4`G, rr�� AM PM
Relinquished by: ` Date: Time: AM PM
Received by. Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date. Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature (°C):
Method of Shipment: ❑ UPS ❑ Fed ESC x Meritech Pick-up ❑ Delivered ❑ Other
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED
Relinquished by:
SAMPLE RECEIVING (Laboratory Use Only1
/'�
Received by: / Date: f 7 Time: /Oa) AMPmt
4
Sample Temperatures Sample Condition:_
WHITE = Laboratory coav YELLOW = Client rnnv

. . . 1 1. 11 1 I
• MERITECH, INC. Q l I S I
MerSample ID #:
Bioassay Sample Chain of Custody
642 Tamco Rd. Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike.reed(a_rnerifech-labs com Web Site: www.meritech4abs.com
CLIENT INFORMATION
Client:TG'� �,� e1QY..J PO#:
Contact Person: -4e, -..r sw 01. xx"d 2 NPDES#: NC QQ,;Zsyi3
Address: PO 4 -79 Phone:
City: C/G�wTo'—, Pipe #:
County; , /Sro� State: AA- Zip:,;1-7.5,7-S!f
Sample Site: L"tr% Cre e -L 00,-;`-t,-
Sample Type: ❑ Grab V�Somposite
Sampling Time: Start Date:'
End Date:
�o•J {mac,-/� ti
# of containers: �-
Start Time: �%� AM _ PM
End Time:%922 AM PM
!'= Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory*"
Collector's Name: Print, ��t�ct yvl S i �^� /SSD n 1 Signature: -11;V40
TOXICITY TEST INFORMATION
Test Required: Chronic (7 days) Test Organism: ,��6eriodaphnia dubia (water flea)
❑ Acute (24-48 hours) kLPimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC:,9, o % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by Date:
Received b _ Date: / S//
Relinquished by �G— Date: C2 f /.51/-7
Received by: Date:
Relinquished by:
Date:
Received by:
Date:
Relinquished by.
Date:
Received by:
Date:
Sample Temperature CC):
Time: 1,2D J� AM
PM
Time: AR O S
AM
Time: / S3 o
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑ Delivered ❑ Other
'* Samples shipped on Friday must be FedEx and must be dearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
Relinquished b . g-
Receiv Date:
1 f '
r r Time: �(1 AM PIU
4
Sample Temperatures CC): ;� � ! 1 ! / Sample Condition: ��

Effluent Toxicity Statistical Results - Chronic Pass/Fail
Date: 06/21/17
Facility: CLAYTON WWTP NPDES#: NCO025453 Pipe#: 001 County:JOHNSTON
Laboratory Performing Test: MERITECH LABS, INC.
Reduction: -1.960
## Replicates
Female Live
Adult Male
Adult Dead
Adult Mortality
## Neonates
Mean # Neonates
Standard Deviation
Coefficient of Variation
CONTROL 2.0% Effluent
12
12
0
0
0.00o
255
21.250
2.832
13.3290
Fisher's Exact Test
A = 12 B = 12 a = 12
a/A = 1.00 b/B = 1.00
Success is: survival
Critical b value = 8
12 > 8
12
12
0
0
0.00%
260
21.667
2.839
b = 12
The test concludes that the proportion of survival is not
significantly different for the control and the effluent groups.
Test Passes!
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA
i Group Neonates
1
E
18
2
C
18
3
C
18
4
E
19
5
E
19
6
C
19
7
C
19
8
C
19
9
E
20
10
E
20
11
E
21
12
E
21
ORDERED
Centered
OBSERVATIONS
i Group
Neonates
Centered
-3.6667
13
C
21
21
-0.2500
-0.2500
-3.2500
14
15
C
E
22
0.3333
-3.2500
-2.6667
16
C
22
0.7500
-2.6667
17
E
23
23
1.3333
1.7500
-2.2500
18
19
C
E
24
2.3333
-2.2500
-2.2500
20
C
24
2.7500
-1.6667
21
C
24
2.7500
-1.6667
22
E
26
4.3333
-0.6667
23
E
27
5.3333
-0.6667
24
C
27
5.7500

SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.)
x(n-i-1) - x(i)
1
W = X 164.5064
176.9167
Calculated W = 0.930 Critical W = 0.884
0.930 z 0.884
normally
istributed
The reroduction evaluated atdat99osconfidence dinterval.
Test Passes!
F test for Homogeneity of Variance
Effluent variance 8.0606 = 1.00
F =
Control variance 8.0227
9.4167
8.5833
7.5833
5.4167
5.4167
4.5833
4.0000
3.5833
2.4167
2.0000
0.4167
0.4167
Numerator degrees of freedom: 11 Denominator degrees of freedom: 11
Critical F = 5.32
1.00 s 5.32 =► The Test PASSES, the variances of the two groups
are significantly the same, homogeneous.
COEFFICIENTS AND DIFFERENCES
x(n-i-1)
x(i)
a(i)
i
1
5.7500
-3.6667
0.4493
0.3098
2
5.3333
-3.2500
-3.2500
0.2554
3
4.3333
2.7500
-2.6667
0.2145
4
5
2.7500
-2.6667
0.1807
6
2.3333
-2.2500
0.1512
7
1.7500
-2.2500
0.1245
0.0997
8
1.3333
-2.2500
-1.6667
0.0764
9
10
0.7500
0.3333
-1.6667
0.0539
11
-0.2500
-0.6667
0.0321
0.0107
12
-0.2500
-0.6667
x(n-i-1) - x(i)
1
W = X 164.5064
176.9167
Calculated W = 0.930 Critical W = 0.884
0.930 z 0.884
normally
istributed
The reroduction evaluated atdat99osconfidence dinterval.
Test Passes!
F test for Homogeneity of Variance
Effluent variance 8.0606 = 1.00
F =
Control variance 8.0227
9.4167
8.5833
7.5833
5.4167
5.4167
4.5833
4.0000
3.5833
2.4167
2.0000
0.4167
0.4167
Numerator degrees of freedom: 11 Denominator degrees of freedom: 11
Critical F = 5.32
1.00 s 5.32 =► The Test PASSES, the variances of the two groups
are significantly the same, homogeneous.

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:3/1612017
Facility. Clayton NPDES # NC00 25453 Pipe#: 001 County. Johnston
rkstiorato`ry: w, ritech, Inc. Commen
x vG R
ature of Operator ip,Respon ibl Charge
x
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
'est Initiation Date/Time 3/7/2017 4:55 PM Avg Wt/Surv. Control 0.872
% Eff. Repl.
1
2
3
4
Control Surviving #
10
10
10
10
Original #
10
10
10
10
Wt/original (mg)
0.957
0.769
0 895
0.865
0.5 Surviving #
9
10
10
10
Original #
10
10
10 1
10
Wt/original (mg)
0.959
0.991
0 804
0.952
o Surviving #
10
9
10
9
Original #
10
10
10
10
Wt/odginal (mg)
0.878
0.783
0.824
0 705
Surviving #
10
10
9
10
Original #
10
10
10
10
Wt/original (mg)
1.002
0 854
0 905 1
0.925
® Surviving #
10
10
9 1
10
Original #
10
10
10
10
Wt/odginal (mg)
0 918
0.865
0.764
1.041
® Surviving #
10
10
10
9
Original #
10
10
10
10
Wt/original (mg)
1.001
0.901
1.024
0.862
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
;onductivity (umhos/cm)
Chlodne(mg/L)
Temp. at Receipt ("C)
% Survival 100.0
Avg Wt (mg) 0.872
% Survival 97.5
Avg Wt (mg) 0.927
% Survival 95.0
Avg Wt (mg) 0.798
% Survival 97.5
Avg Wt (mg) 1 0.922
% Survival 97.5
Avg Wt (mg) 0.897
% Survival 97.5
Avg Wt (mg) 0.947
Day
n t 9 3 4 5 6
Test Organisms
C Cultured In -House
Outside Supplier
Hatch Date: 3/6/17
Hatch Time: 3:00 pm CT
8.09 / 7.87
8.14 / 7.62
8.09 / 7.86
8 14 / 7.78
1 8.15 / 8.12
8.24 / 7.64
8.09 / 7.65
7.67 / 7.01
7.76 / 6.65
1
7.98 / 7.11
7.73 / 7.411
8.00 / 7.26
7.75 / 6.17
7.87 / 6.21
25.8 / 25.3
24.4 / 24.5
24.2 / 24.8
24.9 / 24.7
124.8 / 25.4
124.7 1 25.4
124.7 / 24.9
n .1 2 3 4 5 6
8.02 / 793
811 / 7.63
8.01 / 7 85
8.06 / 7.73
8.06 / 8.14
8.22 / 7.75 I
8.07 / 7.64
7.50 / 6.90
7.93 / 6.10
7.88 / 7.05
7.83 1 6.94
8.01 / 7.55
7.72 / 6.60 17.73
/ 6.10
25.2 / 25.3
24.2 / 24.5
25.0 / 25 3
24 9 / 24.8
24.8 / 25.0
24.8 / 24.7 1
25.0 / 25.6
2 3
3/6/2017
3/8/2017
3/9/2017
Normal
Fill
RfC ChV >8
24.0
22.6
26.8
96
100
98
78
86
91
687
676
651
<0.1
<0.1
<0.1
2.2
1.4
1.1
Dilution H2O Batch # 1131 1132 1133 1134
Hardness (mg/L) 46 43 42 44
Alkalinity (mg/L) 54 53 53 49
:onductivdy (umhos/cm) 196 202 192 199
nwn 9hrr" AT -R r11ndl
Conc. Critical Calculated Critical Calculated
0.5 10 16 2.41 -0.9348
1 10 14 2.41 1.2577
2 10 16 2.41 -0.8498
4 10 16 2.41 -0.4334
8 10 16 2.41 A.2832
Survival
Growth Overall Result
Normal
Fill
RfC ChV >8
Hom. Var.
r(
1-V
NOEC
8
8
LOEC
>8
>8
ChV
>8
>8
Method
Steeps
Dunnetts
Conc. Critical Calculated Critical Calculated
0.5 10 16 2.41 -0.9348
1 10 14 2.41 1.2577
2 10 16 2.41 -0.8498
4 10 16 2.41 -0.4334
8 10 16 2.41 A.2832

Test Termination Data:
3/14/17
Initials/Signature:
End Date:
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
f •���
Client:
Clayton
# of Organisms per Chamber: 10 Start Date: 3/7/17
Time:
I �as
NPDES #: 0025453
Test Vessel Size: 400 ml End Date: 3/14/17
Time:
Initiated by:--;C-'
Test Solution Volume: 250 ml
Date/Time Fed: 3/7/17 ��� P
Tem of Stock:_ °C Randomization: Yes No
p
#:
Date/Time
Born: 3/6/17 3:00 PM CT
Incubator
# of Reps: 4
Organism Source: Aquatox, Inc.
Analyst(s): MR, CD, LV, SB
Transferred by:
Fed by:
Date Time Initials
Date Time 1 Initials Time 2 Initials
1
3/8 �l v1
Day 0 3/7 ;® .�'' 1k Cb
Day
S t�
L.V
Day 1 3/8 Ly
Day2
3/ 9 -�-"—
3/10 ',; µ'/ �1 %LI/
Day 2 3/9 d"►
Day 3
Day
3/11 D
c�
Day3 3_ /10
5
3/12
Day 3/11
Day
3 13 ����,„ L✓�/�
/ �•l�G�
Day 5 3/12
Day
Day 3/13
Test Termination Data:
3/14/17
Initials/Signature:
End Date:

ENVIRONMENTAL Um-BOR,4TOR
.� A Division cf WaYer Technology and Controls Inc-
Chemical
nc
Chemical and Physical Determinations
Client: Cla on t4PDES#: 0025453 StartDate: 3/7/17
End Date: 3114/17
Test Organism: Pimepha►es promelas
1-2
2-3
,may
3-4
4-5
5-6
6-7
Remarks
1133,
)'33
fl - =-I-:2-
1131
9- o el
i I
�-,
47�.
� �
pT-11
-M9
Ind
cif. ti
9
4--
1-7
el

-
/VIER/TECH, i/VC_
4-5
' �P
6-7
Remarks
ENVIRONMENTAL LABORATORIES
A OJVJdo.s of Wseo,',bch,.o/opY and Co..�/o IS- I a
Residual Chlorine: ZCIAi t
Chemical and Physical Determinations
Hardness: r
Client: Clayton
NPDES#:
0025453
. ;
StartDate:
3/7/17
Test Organism: Pimephales promelas
End Date:
3/14/17
Day
Concentration:
2.0%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
pH:
Initial
,
4
�i O (
IMA).
7-% (bFinal
r 7
�'
J
�,
D.O.:
Initial
Mflz1-LjL
`a!
Final
S -
r 6�
.6
t,(
0��7 • C
Temp.:
Initial
Final
��i ,(.�
„K-�
,�7
�7 5
a k
(C0
Conductivity:
Initial
�'
Final
a 1
J 3L-7
Az
Residual Chlorine:
l p, i
<c c
Day
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Concentration:
4%
7,
L
5�-c UY�(I
I
pH:
Initial
7ti
Zl�
31t
Final
'I
j
D.O.:
InitialZ,,e
Final
Temp.:
nitial
i
? Lf
Final
gC
9-
2`•
Conductivity:
Initial
'3
ii
v l
Finalk
a
Residual Chlorine:
2,0 t
Day
i
Concentration:
8.0%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
zol
0&
�-U
PH:
Initial
Final.
�,
"j ,
v? •
7 3
—
D.O.:
Initial
Final
f ►
ip_
U�
Lor `1
ttPi�
o
Temp.:
Initial
U
9L 11 `1
Final
oZ " 3
y l
5 -�
-�- 1 Ly
Conductivity:
Initial
L�
`
�
��
a�z
C
Final
Residual Chlorine:
Cn
G �t I
G rn.
L Jl F
ti
Page 2 of 2
Remarks
Remarks
Remarks
Concentration: 100% 0-11-2 2-3
Lu.Y
3-4
4-5
5-6
6-7
Remarks
pH:
Conductivity:
Residual Chlorine: ZCIAi t
iZ
Hardness: r
Alkalinity: %� I
. ;
-c — 1 L. --0

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Clayton
Test Organism: Pimephales promelas
Concentration Control
NPDES#: 0025453
Start Date: 3/7/17
End Date: 3114/17
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
6 o
Rep B
0- 0 0
Rep C 0
C) G o b
Rep D 0 o o - -
Concentration
0.50%
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
v
O
0
Rep B
I
Rep C
— --
I Rep D
0
iConcentration 10_0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep
Rep B
Rep C 0
V
Rep D

MERITECH, ING.
Mortality Data: Chronic Fathead Test
Client: Clayton NPDES#: 0025453 Start Date: 3/7117
Test Organism: Pimephales promelas End Date: 3/14/17
Concentration 2.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Concentration
4%
--�— --�-- --� — --0--
Rep B 0
Rep A 0
Rep C 0
lJ tai
Rep B 0
Wednesday
Thursday
Friday
Saturday
Rep C 0
Monday
Tuesday
Rep 0�
Concentration
4%
--�— --�-- --� — --0--
Rep B 0
Rep C 0
lJ tai
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
0
Rep B
Rep C
0
l
Rep
0
--� -
Concentration 8.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
) _
--�— --�-- --� — --0--
Rep B 0
Rep C 0
lJ tai
o
Rep 0
_

Facility: Clayton_
NPDES #: 0025453
AffERI TECH'r 1 C.
ENVIRONMENTAL LABORATORIES
A OivisiOn Of Water Technology and COntrO%s. /nc
FISH LARVAL SURVIVALIGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Date of Test: 3/7/17
Initial weights taken on: by:
Final weights taken on: 3by:
Page: 1 of 2
Outfall:
Organism: Pimephales promelas
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV %
CONC
REP
, qC3
05sT 0a
5 7
ID
v. qS7
100�
A
B
$ U
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a 76
10
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D
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A
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g /. n
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1 ��
C
U�
D
1 �
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"�► DT,��,
��
%�
0-7050
D

ERI 'EC S IMC -
ENVIRONMENTAL LABORATORIES
.`r A Division of Water Technology and Controls. Inc_
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Clanton
Page: 2 of 2
NPDES #: 0025453 Outfall:
Date of Test: 3/7/17
Initial weights taken on: - a�_ R by: < Organism: Pimephales promelas
Final weights taken on: %5-17 by: LAI—
PAN
✓
PAN + ORG.
WT. (mg)
'33
WT. OF ORG.
(mg)
# ORG.
Cry
MEAN
WTJORG. (mg)
1, 06a.
(),�5
SURV %
too
goo
qo
/00
00
10 0/
q0
ton
100
too
CONC
REP
PAN WT.
(mg)
2.0%
o
4 /o
A
lk,
B�o
9L-10-�L�
Lt
WSa.rl
-el-K, 6 7
�pi�>, F
q. 66
b /
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to
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D
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U
B
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I Rqq,q5jF:�3-�7,

MERITECII, INC.Merttwnsampiu lD#'
_
Bioassay Sample Chain of sfody
642 Tamco Rd. Reidsville NC 7320
Phone: [phone] Fax: 1-.:
Toxicity Supervisor email: [e-mail]. Web Site: www.meritech-labs.com
Client f27- l -!'l
Contact Person: J^GLv-� -e-,S
Address:
City: r in, � 2 a
County: �� jn t,,, 7
Sample Site: 1-i 271 -0— C
Sample Type: ❑ Grab Composite
State:
PO#:
NPDES#: NC 49a.45'20
Phone: 17/%
Pipe # Qom/
Zip:
# of containers'
Sampling Time: Start Date: -7 6-/-7 Time: l� AM PM
End Date:` 1 /
End Time: A PM
*** Triple rinse sample container with
sample before filling- Comple ly fr►/ the sample container with no air space.
pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory***
Collector's Name: Print
Signature'
TOXICITY TEST INFORMP TION
Test Required:hronic (7 days)
Test Organism: -D!7tce,!4 Pdaphnia dubia (water flea)
❑ Acute (24-48 hours)
Pim pha/es promelas (fathead minnow)
❑ mys dopsis bahia (shrimp)
IWC: %
Test Concentrations (if r iultiple dilutions):
Comments:
LL
SHIPPING INFORM ATI N
Time: 0�/0
�,/;)
Al (1 5
Relinquished by
Date: --�
Date: Time: /�'h
AM PM
Received b ' ��-
�—
�?
'�
% Time:
Date: Z �/ �=
AM M
Relinquished by:
_�
Time:
AM PM
Received by
Date:
Time:
AM PM
Relinquished by:
Date'
Time:
AM PM
Received by
Date:
Time:
AM PM
Relinquished by
Date:
Time:
AM PM
Received by:
Date:
Sample Temperature (`C):
Method of Shipment F-1 UPS
❑ Fed EX �q_Medtech Pick-up ❑ Delivered [] Other
*• Samples shipped on Friday must be FedEx and must be dearly labeled for Saturday delivery, NO SIGNATURE REQUIRED *`
Relinquished by. f ' t
Received by Date:
' Ti".meAM CM
Sample Temperatures (°C): : / // / Sample Condition: r
WHITE = Laboratory copy YELLOW = Client copy

MI�FtITEC�'17 INC- Mentech sample ID #
Bioassay Sample Chain of custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
ta�meritech-labswa
Web Site: vw.meritech4abs.com
Toxicity Supervisor email' mike reed
CLIENT INFORMATION
Client: 1%2!�/`� �T NPDES#: NCdp,Z.r�iS�
Contact Person: J //
Phone.
Address: / 79 Pipe #: 00/
City: [' /czYfn� zip: uriz c�
County:
TOl,e✓« � state: c -
SAMPLE INFORMATION
Sample Site: Ll f -7h ti 4ZLe-
Samplele Type: ❑Grab osite # of containers P
Sampling Time: Start Date: '� — R � �%
Start Time: L- ® PM
End Date: End Time: oe ss A PM
Completely fill the sample container with
***Triple rinse sample container with sample before filling_ no aim SAa�e•
ust be < 6.t)°C upon receipt at the laboratory*"
pack the sample cooler completely in ice The sample m
/t Signature: y
Collector's Name: Pnnt_(J %l' a" /'� sa.rr P-so-�
TOXICITY TEST INFORMATION
Ca, Test organism: � CerFodaphnia dubia (water flea)
Test Required: -Chronic (7 days)
❑ Acute (24-48 hours) 5?Lpimepha1es promelas _(fathead minnow)
❑ Mysidopsis bahia (shrimp)
SWC• %
Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
AM M
11
by:
Date: 3 ��rl
3
Time*i
Time:�Q '7
TPMRelinquished
AMDate:
Received by:
sAM
Time: �—
Relinquished by ADate:
Time: _
AM PM
Received by:
Date:
Time:
AM PM
Relinquished by
Date:
Time: __
AM PM
Received by:
Date:
Time:
AM PM
Relinquished by:
Date:
_`
Time: �—
AM PM
Date:
Received by:
Sample Temperature CC):
Meritech Pidc-up ❑ Delivered
Method of Shipment:[] UPS ❑ Fed IX �, _
NO
F1 Other__
SIGNATURE REQUIRED
"
be FedEx and must be dearly labeled for Saturday deirvery,
"Samples shipped on Friday must
A
SAMPLE RECEIVING (Laboratory Use Onl
Relinquished by:
Received by.
Sample Temperatures CC): _-j}_�'
Time: G{ AM_7/
M ,
ate:
i L i i Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

MERITECFI, INC.
Bioassay Sample Chain of Custodsr
m 642 Tamco Rd, Reidsville NC 27320
r
% Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike reedCa?mentech-labs.com Web Site-. www.meritech4abs.com
Polk
Client: , .. ,l NPDEs#y5,3
Contact Person:
Phone.
Address:
Pipe #:0—�
County: �r �S s✓ State: Ati _ Zip: -
Sample Site: i `X I r X %,I- ?
Sample Type:
E] Grab Composite # of containers: V _
Sampling Time: Start Date:'
Start Time -PM
�l f� End Time. � PM
End Date: � _.
*** Triple rinse sample container with sample before filling. Completely fill the sample container with no air xspace-
pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the
iaboorratory
Collector's Name: Print:14/4 &4i vel ! r��r�Signature:,;- i � - �^l
TOXICITY TEST INFORMATION
Test Required- Chronic (7 days) Test Organism: �Ceriodaphnia dubia (water flea)
❑ Acute (24 48 hours) T Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC:p;Zr O % Test Concentrations (if multiple dilutions)
Comments:
Relinquished by:
Received by:
Relinquished by:
Received by.
Sh' --f-r-1UPS
-'F
Date:
Date:
Date:
Date:
Sample Temperature (°C):
Time:
SHIPPING INFORMATION/17
PM
Date: /
Relinquished by: +
Date: h:2
Received b
-3
Date• 3
Relinquished by'�—��-
Time:
Received by
Date:
Relinquished by:
Received by:
Relinquished by:
Received by.
Sh' --f-r-1UPS
-'F
Date:
Date:
Date:
Date:
Sample Temperature (°C):
Time:
OA,
PM
Time:
Time:
i r ss
O 5 AM
PM
Time-
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
❑ Fed EX E]Meritech Pick-up F] Delivered E]Other
Method
ip
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: I
Date: 31 I o { I Time: -Lk(L,5—' AM PM
Received by: -
} i Sample Condition: 0-/% 1 CL'
Sample Temperatures (°C): `
1111111- WHITE = Laboratory copy YELLOW =_ Client copy

,it le: Clayton Transform: NO TRANSFORMATION
vile: C1ayGrowth
Shapiro - Wilk's Test for Normality _------_-r_------
D = 0.1246
W = 0.9686
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

itle: Clayton Transform: No TRANSFORMATION
ile: C1ayGrowth
Bartlett's Test for Homogeneity of Variance---------
---------------------------------
'alculated B1 statistic = 1.2410 (p-value = 0.9409)
)ata PASS B1 homogeneity test at 0.01 level. Continue analysis.
---------------------------------
:ritical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)

Clayton ton NO TRANSFORMATION
litl'ilee. y C1ayGrowth
Transform:
ANOVA Table ---------------------
SOURCE
DF
SS
MS
---------------
---------------------- 1.6693
------5 -
"'-------------- 0.0578 0.0116
Between
I 18 0.1246 0.0069
Within (Error)----------
---------23--
---Total----- __ _____
0.1824
-------------
--------------------
- (p -value = 0.1930)
Critical F _ 2.7729 (alpha = 0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title:
Clayton
NO TRANSFORMATION
Citle:
Clayton
ClayGrowth
Transform:
?ile:
Ho:Control<Treatment
Dunnett's Test -
TABLE 1 OF 2
--
---------
------------------
- -----------------------------------------
TRANSFORMED
MEAN CALCULATED IN SIG
T STAT 0.05
------------------
----'------
MEAN
UNITS
GROUP
IDENTIFICATION
IDENTIFICATION
--ORIGINAL
----
1
------------- Cont---
Control
---------
0.8715
0.8715
0.9265 -0.9348
2
0.50
0.9265
0.7975 1.2577
3
1.
0.7975p
0,9215 -0.8498
4
2,.0
0.9215
0.8970 -0,4334
5
g
0.8970
0.9470
0.9470 -1.2832
6
8.0
4
-------
Dunnett
--------------
critical value = 2.4100
(1 Tailed,
alpha = 0.05, df = 5,18)
Title:
Clayton
Transform:
NO TRANSFORMATION
File:
ClayGrowth
Ho:Control<Treatment
Dunnett's Test -
TABLE 2
OF 2
------------------
----'------
NUM OF
-------------------
MIN SIG DIFF
UNITS)
% OF DIFFERENCE
CONTROL FROM
GROUP
IDENTIFICATION
REPS
-CONTROL
(IN ORIG.
Control
4
0.1418
16.3 -0.0550
2
0.50
4
4
0.1418
16.3 0.0740
3
1.00
0.1418
16.3 -0,0500
4
2.0
4
4
0.1418
16.3 -0.0255
5
4
4
0.1418
16.3 -0.0755
6
8.0

Title: Clayton Transform: NO TRANSFORMATION
File: ClaytonSury
Shapiro - Wilk's Test for Normality --------------
----------------------- --------------------------------------
D = 0.0400
W = 0.7712
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning - The first three homogeneity tests are sensitive to non -normality
and should not be performed with this data as is.

Title: Clayton NO TRANSFORMATION
File: ClaytonSury Transforms
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
-----------------------------------------------------------------
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.

D .
Title: Clayton
File: ClaytonSury Transform: NO TRANSFORMATION
----------------------------------------------------------------------------
Steel's Many -One
Rank Test
- Ho:
Control<Treatment
MEAN IN
RANK
CRIT.
SIG
GROUP
IDENTIFICATION
ORIGINAL UNITS
SUM
VALUE
DF 0.05
-------------------------
1
Control
-----------
1.0000
-------
------
----- ---
2
0.50
0.9750
16.00
10.00
4.00
3
1
0.9500
14.00
10.00
4.00
4
2.0
0.9750
16.00
10.00
4.00
5
4
0.9750
16.00
10.00
4.00
6
8.0
0.9750
16.00
10.00
4.00
----------------------------------------------------------------------------
Critical
values are 1 tailed ( k = 5 )

TOWN OF CLAYTON
"SERVICE' OPERATIONS CENTER 'ENVIRONMENI''
ELECTRIC SERVICE • PUBLIC WORKS
[phone] [phone]
VEHICLE MAINTENANCE WATER RECLAMATION
[phone] [phone]
March 24, 2017
-Water Sciences Section
Aquatic Toxicity Branch
DWR
1621 Mail Service Center
Raleigh, NC 27699-1623
Re: Toxicity Results: NPDES# NCO025453
To Whom It May Concern:
Enclosed please find the Ceriodaphnia Pass/ Fail toxicity and Chronic Fat Head Minnow
results for the Little Creek Water Reclamation Facility.
Please call me directly at 919-553-1536 if you have any questions.
Sincerely, fn,
es W, ORC
Town of Clayton, NC
653 Highway 42 West - P.O. Box 879 - Clayton, North Carohna 27520 - (9I9) 553-1530 - Fax [phone]

st.F4:1iiPnt Toxicity Report Form - chronic pass/Fail and Acute LC50
Date: 03/16/17
mnLT
NPDES#: NCO025453 Pipe#: 001 C.,r,,..
ountry: u'
Facility: CLAYTON WWTP
Laborat y Perf rming Test: MERITECH LABS, INC. Comments:
X �'J a� I * PASSED: -2.25$ Reduction
Signature o L oratory upervisor
ork Order: Environmental Sciences Branch
MAIL ORIGINAL TO: Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
orth Carolina CeriodaphniaChronic Test Results
Chronic Pass/Fail Reproduction Toxicity Test Calculated t = -0.715
Tabular t = 2.508
n*1mu1r)T. CWMANTSMS 1 2 3 4 5 6 7 8 9 10 11 12 t Reduction = -2.25
I
## Young Produced 20 24 21 23 23 22 23 24121120 22 24
Adult (L) ive (D) ead L L L L L L L L L L L IL
ffluent W: 2.0%,
+f REATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
## Young Produced 26 23 23 19 22 22 21 25 22 22 23 25
Adult (L) ive Mead L L L L L L L L L L L L
7 Mortality
Avg.Reprod.
0.00
22.25
Control
Control
0.00
22.75
Treatment 2
Treatment 2
Control CV
6.673 PASS FAIL
control orgs ECheck
producing 3rd
brood One
100
1st sample 1st sample 2nd sample Complete This For Either Test
Fi Test Start Date: 03/08/17
Control 8.05 8.11llection (Start) Date
8.00 8.06 7.99 8.12 Co
Sample 1: 03/06/17 Sample 2: 03/08/17 2nd
reatment 2 8.08 8.06 8.09 8.02 8.01 8.05 Sample Type/Duration 1St P/F
s s s Grab Comp. Duration D
t e t e t e I S S
h
a n a n a n Sample 1 X 23.0 rs U M M
r d r d r d
X 23.6 hrs T P P
t t t Sample 2
1st sample 1st sample 2nd sample Hardness (mg/1) 44 ........ .........
0.
Control 7.83 7.59 8.18 7.49 8.12 7.23 Spec. Cond.(pmhos) 183 687 676
reatment 2 7.74 7.50 7.82 7.48 8.10 7.22 Chlorine(mg/1) <0.1 <0.1
LC50/Acute Toxicity Test Sample temp. at receipt(OC) ........ 2.2 1.4
mr-raiity P3mressed as %, combining replicates) N0 te• Please
Concentration Complete This
Section Also
Mortality
start/end start/end
LC50 = -w Method of Determination
95-* Con i ence Limits Moving Average -Probit
% -- % Spearman Karber _ Other
pH
Organism Tested: Ceriodaphnia dubia Duration(hrs):
7opied from DWQ form AT -1 (3/87) rev. 11/95 (DUBIA ver. 4.41)
•
Control
High
pH
Organism Tested: Ceriodaphnia dubia Duration(hrs):
7opied from DWQ form AT -1 (3/87) rev. 11/95 (DUBIA ver. 4.41)
•

Dav #2 1 2 3 4 5 6 7 8 9 10 11 12
#Young Produced
0
Meritech, Inc.
Duration
3
Client
Mini Chronic Pass/Fall Test Cenodaphnia dubia
Pipe # Gp County '-50- �,S+&A Date Start �-
Incubator
Date End.
#
3
NPDES #. NCya,)� `l'; � Date / Time of Culture Transfer•_%/•7
Z L�=� �1,,n
Time Start -'
Time End;
Adults Live / Dead
Dilution Water: Lake Brandt
Date / Time Neonates born. j
�n-S.'
1st Renewal Date: g�j 0�1 �-
Time:
;
Test Organism Source- Tray # Li Age oft
N eonates at Test Start,
Stirred / Aerated for D.O. Y / Randomized:Y / N Culture Tray
hours
Temp. J - °C
2nd Renewal Date, -3�) 3 -0 Time:
Analyst(s). MR, co, uv, ss Reviewed by-
3 PAIn
/AL_
Dav #2 1 2 3 4 5 6 7 8 9 10 11 12
#Young Produced
0
00
Duration
0
0
0
0
0
0
0
0
0
Adults Live / Dead
L
Lr
L
L
'L
L
L
I it
i--
t_
t—
L
Dav #5 1 ,2. 3 4. 5 6 7 8. 9 10 11 12
Dav #7 1 2 3 4 5 6 7 8 9 10 11 12
#Young Produced
100% H
G / C?
Duration
Sample 1
Sample 2
S V hours
)
% l
j
1
/02
Adults Live / Dead
L
L
L
L
L
L-
L
L
I—
L.
L-
I (_
I Total Produced I �-b 12 � I '; i I -,� _� I ?31'n I al Ic y I Ic,�/) I qa 1-1-gI
Sample 1: 3 " _ t Sample 2• --�`
Sample Information
Batch #
100% H
G / C?
Duration
Sample 1
Sample 2
Transfer Day
S V hours
Sample 2
5
:.
hours
Batch #
-
q-5-
[aNN�
0'
Sample 1
Sample 2
Transfer Day
0
2
5
:.
' ►
Hardness
Adults Live / Dead
L,
L
Z-
L
(mg/L)
L
L-
(_
�I
,', `
Spec. Cond.
,
1
/
(umhos/cm)
(D
Temp ('C)
Percent of Control producing third brood, %
Chlorine
Test Sample Ornanism Reproduction Receipt Sample
Effluent
Dav #2 1 2 3 4 5 6 7 8 9 10 11 120 pH 1st Sample
# Young Produced
0
[aNN�
0'
ajV,Id
00
S%i
0�
MAN
RVA
' ►
h
Adults Live / Dead
L,
L
Z-
L
L
L-
(_
L
Dav #5 11 2 3
un - .. e
cel'i
[aNN�
:00000
ajV,Id
MR
S%i
0�
MAN
RVA
' ►
h
Adults Live / Dead
Z-
L
L
L-
(_
L
un - .. e
cel'i
[aNN�
Sample
ajV,Id
MR
S%i
0�
MAN
RVA
Adults Live / Dead
Z-
L
L
L-
(_
L
Dav 97 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced
(
1-s
Sample
j(�
j j
/ J
1 f
�)
Adults Live / Dead
Z-
L
L
L-
(_
L
Total Produced 12\
Comments
Control
r
1-s
Sample
inmei nnai
Temp. 1 st Sampie 11
Control
Sample
2nd Sample
imus mal
2nd Sample
initial ns
2nd Sample
Transferred by Fed by
Day 0 ,/� LV
Day 1
Day 2 Mr !,V
Day 3 f�
Day 4 Sl
Day 5
Day 6
Terminated by/"_S1,_
2nd Sample
"M17
E I
n, is ,ma
2nd Sample
id- n
n ua 1na
2nd Sample
ate tic_ �

MERITECH, INC. Mentech Sample ID
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike reedOlmeritech4abs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client fJG-✓ •r/
2 � CA 7 7`L�.-+�
PO#:
Contact Person:
TGL.�t.-G.,.1
/.t,G d'y'-G�*�
NPDES#: NC
ox
o
Phone: �%/ 7- ss3—Is`3!o
Address:
City:
/0L VYTi
Pipe #: &;V/county:
U C— -+ ' �
A
� State. Svc— Zip:
SAMPLE INFORMATION
Sample Site:
) .//�
L-i / j�
Sample Type:
❑ Grab
Viromposite # of containers,
I—
Sampling Time: Start Date: -7 `/p -L / Start Time: AM PM
End Date: ` End Time: A PM
Triple rinse sample container with sample before filling. Completely rill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory'"**
Collector's Name: Print.
Test Required: 9hronic (7 days)
❑ Acute (24-48 hours)
IWC:
Comments:
Signature:
TOXICITY TEST INFORMATION
Test Organism: �eriodaphnia dubia (waterflea)
XPimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions).
SHIPPING INFORMATION
Relinquished by. Date: s -'%-
Received b ' ��� Date:�y3� -7-h?�—
Relinquished by: �— Date: .cZ-7// 7
Received by: Date:
Relinquished by:
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Date -
Date:
Date:
Date:
Sample Temperature CC):
Time: glo
Time: -�
Time: 63 d
Time:
Time:
Time:
Time,
Time:
Method of Shipment:❑ UPS ❑ Fed EX $Meritech Pick-up ❑ Delivered ❑ Other
Alvl P�
AM( PM
AM /PM
AM �PM
AM PM
AM PM
AM PM
AM PM
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED'"
Relinquished by:
CMReceived by: Date: . Time: �AM
Sample Temperatures CC): 6�1 �/ / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

AY
MERITECH, INC. Mentech Sample ID # ``� o
Bioassay Sample Chain of Custody
- 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax [phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
I CLIENT INFORMATION
Client: 12217� PO#-
NPDES#: NC �jD,2,SN$3
Contact Person: �� rKLS G✓� // -r "J
Address:—p0�l�— S79 Phone.
City:Pipe
County: Tj9A AS fry.-J State: /(%Ci zip:2'1sz
SAMPLE INFORMATION
Sample Site:
Sample Type: ❑ Grab ®-Composite # of containers-
Sampling Time: Start Date: % ? Start Time:
PM
End Date: 3' !� / % End Time: L')d ss PM
*'* Triple rinse sample container with sample before filling_ Completely fill the sample container with no airs ace.
Pack the sample cooler completely in ice_ The sample must be < 6.0°C upon receipt at/the laboratory'`'"
Collector's Name: Print: (�%► /�� Cw� $ �'� 4O'-'� Signature.
TOXICITY TEST INFORMATION
Test Required: -Chronic (7 days) Test Organism: CatC,,eriodaphnia dubia (water flea)
El Acute (24-48 hours) f-Pimephales promelas (fathead minnow)
Mysidopsis bahia (shrimp)
IWC: % Test Concentrations (if multiple dilutions):
Comments'
SHIPPING INFORMATION
Date: 3 ��rl �_ Time: f Z D AM
OM
Relinquished by. ' Z % AMTPM
Date: 3 Time: �Received by: s ZSRelinquished byDate Time. AM
by:
Date: Time: AM PM
Received
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Date* Time. AM PM
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Date: Time: AM PM
' Date: Time: AM PM
Relinquished by
Date
Time: AM PM
Received by
I
Sample Temperature CC).
Method of Shipment: ❑ UPS ❑ Fed EX g. Meritech Pick-up ❑ Delivered U Other
"Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
{ Received by.
ate: 31,11,Time: !/ AM CM
L l 1 , L I 1 Sample Condition:
Sample Temperatures CC)
WHITE = Laboratory copy YELLOW = Client copy

Effluent Toxicity Statistical Results - Chronic Pass/Fail
Date: 03/16/17
Facility: CLAYTON WWTP
NPDES#: NCO025453 Pipe#: 001 County JOHNSTON
Laboratory Performing Test: MERITECR LABS, INC.
Fisher's Exact Test
A = 12
B = 12 a = 12 b = 12
a/A = 1.00 b/B = 1.00
Success is: survival
Critical b value = 8
12 > 8
The test concludes that the proportion of survival is not
significantly different for the control and the effluent groups.
Test Passes!
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA
i Group Neonates
1
CONTROL
2.0% Effluent
Reduction: -2.250
C
20
# Replicates
12
12
Female Live
12
12
Adult Male
0
0
Adult Dead
0
0
Adult Mortality
0.0016
0.00%
a
267
273
# Neonates
E
22
Mean # Neonates
22.250
22.750
Standard Deviation
1.485
1.913
Coefficient of Variation
6.673%
22
Fisher's Exact Test
A = 12
B = 12 a = 12 b = 12
a/A = 1.00 b/B = 1.00
Success is: survival
Critical b value = 8
12 > 8
The test concludes that the proportion of survival is not
significantly different for the control and the effluent groups.
Test Passes!
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA
i Group Neonates
1
E
19
2
C
20
3
C
20
4
E
21
5
C
21
6
C
21
7
E
22
a
E
22
9
E
22
10
E
22
11
C
22
12
C
22
ORDERED
OBSERVATIONS
Centered
i Group
Neonates
Centered
-3.7500
13
E
23
23
0.2500
0.2500
-2.2500
14
15
E
E
23
0.2500
-2.2500
16
C
23
0.7500
-1.7500
-1.2500
17
C
23
0.7500
-1.2500
18
C
23
24
0.7500
1.7500
-0.7500
19
20
C
C
24
1.7500
-0.7500
-0.7500
21
C
24
1.750u
-0.7500
22
E
25
25
2.2500
2.2500
-0.2500
23
E
E
26
3.2500
-0.2500
24

SHAPIRO-WILK'S TEST FOR NORMAL
DISTRIBUTION OF DATA (cont.)
i x(n-i-1)
1
3.2500
2
2.2500
3
2.2500
4
1.7500
5
1.7500
6
1.7500
7
0.7500
8
0.7500
9
0.7500
10
0.2500
11
0.2500
12
0.2500
AND DIFFERENCES
COEFFICIENTS
a(i)
x(i)
x (i)
-3.7500
0.4493
7.0000
4.5000
-2.2500
0,3098
0,2554
4.5000
-2.2500
0,2145
3.5000
-1.7500
0,1807
3.0000
-1.2500
0,1512
3.0000
-1.2500
0,1245
1.5000
-0.7500
0.0997
1.5000
-0.7500
0,0764
1.5000
-0.7500
0,0539
1.0000
-0.7500
0,0321
0.5000
-0.2500
0,0107
0.5000
-0.2500
W = 1 X 63.3799
64.5000
Calculated W = 0.983 Critical W = 0.884
0.983 a 0.884
The revaludata
slnormally
distributed
ated ata99- confidenceinterval.
Test Passes!
F test for Homogeneity of Variance
Effluent variance 3.6591 _ 1.66
F -
Control variance 2.2045
Numerator degrees of freedom- 11 Denominator degrees of freedom: 11
Critical F = 5.32
1.66 s 5.32 =► The Test PASSES, the variances of the two groups
are significantly the same, homogeneous.

EQUAL VARIANCE t TEST
22.3 - 22.8_ -0.715
t =
0.699
Degrees of freedom = 22
Critical t = 2.508
-0.715 < 2.508
Test passed. There is not a significant difference in reproduction
between the Control and the effluent evaluated at a 99% confidence interval.
Chronic Test PASSES

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 12/21/16
Facility: CLAYTON WWTP NPDES#: NCO025453 Pipe#: 001 County: JOHNSTON
Labor, y Performing Test: MERITECH LABS, INC. Comments:
r
I X � - i cvv
Signature o L oratory Supervisor
* PASSED: -12.821 Reduction
Work Order: Environmental Sciences Branch
MAIL ORIGINAL TO: Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodaphnia Chronic Test Results
Chronic Pass/Fail Reproduction Toxicity Test Calculated t = -2.710
Tabular t = 2.508
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 t Reduction = -12.82
# Young Produced II23I25I26I24I18I22I23 23125123126115
Adult (L)ive Mead IIL IL IL IL IL IL IL IL IL IL IL IL
t Mortality
Avg.Reprod.
0.00
22.75
Control
Control
0.00
25.67
Treatment 2
Treatment 2
Effluent t: 2.Ot
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Control CV PASS FAIL
# Young Produced 24 28 24 27 28 26 23 27 23 27 26 25 t control orgs X
producing 3rd
brood Check One
Adult (L) ive (D) ead L L L L L L L L L L L L 1001
For
1st sample 1st sample 2nd sample Test Startplete Date:This 12/14/16Either Test
pH
Control 7.95 7.95 7.93 8.10 8.04 8.11 Collection (Start) Date 2. 12/14/16
Sample 1: 12/12/16 Sample2nd
Treatment 2 7. 7.92 7.95 8.09 8.09 8.08 Sample Type/Duration
98
1st P/F
s s s Grab Comp. Duration D
S S
t e t e t e I
a n a n a n U Sample 1 X 25.2 hrs M M
r d r d r d
t t t Sample 2 X 23.4 hrs T P P
1st sample lst sample 2nd sample .........
Hardness(mg/1) 42
D.O. .
Control 7.95 7.73 7.94 7.83 8.05 7.65 Spec. Cond.(pmhos) 139 567 669
Treatment 2 8.04 7.85 7.94 7.85 8.13 7.75 Chlorine(mg/1) <0.1 <0.1
Sample temp. at receipt(OC) qPlease
0.9
.......
LC50/Acute Toxicity Test
A,n,rARsed as t, combining replicates) Note
t
t
t
t
t
t
t
t
Concentration Complete This
Section Also
Mortality
start/end start/end
IjC50 = Method of Determination
95t Con l ence Limits Moving Average _ Probit _
$ -- t Spearman Karber _ Other
pH
Organism Tested: Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT -1 (3/87) rev. 11/95 UJUBIA ver. '*.*1i
Control
High
r"nn n _
pH
Organism Tested: Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT -1 (3/87) rev. 11/95 UJUBIA ver. '*.*1i

Meritech, Inc.
Mini Chronic Pass/Fall Test Cenodaphnia dubta J // Incubator #
Client: I �l_(� d% Pipe # (x�r County j-�hnS&n�'� - Date Start I c�-� -1(o Date End.
NPDES # NC���Sj
Date / Tlme of Culture Transfer 7�`�3 r Time Start %(,' ,� Time End. 2,4
Dilution Water: Lake Brandt Date /Time Neonates born /ja'jDp
1+)1st Renewal Date Time
Test Organism Source Tray # Age of eonates at Test Start �, U hours 2nd Renewal Date /� - JQ- 1 Time
Stirred / Aerated for D O Y / N Randomized UY / N Culture Tray Temp• ;�2°C Analyst(s) MR, CO, LV, se Reviewed by
Control Organism Reproduction Collection (Start) Dates
Day #2 1 2 3 4 5 6 7 8 9 10 11 12 Sample 1/�_ /,�T_ Sample 2
P#EYo:u n_ Produced 0 0 1 0 0 0 0 0 0 0 0 0 0
Sample Information
T.... uG 1 1) 1 a r, R 7 R 9 10 11 12
# Young Produced
�
'�
S
�(
a f
5
7
0 0
�
tim
SY
Adults Live / DeadL-
L
t—
I -I
L..
L
L_
L--
t--
L
L
I
T.... uG 1 1) 1 a r, R 7 R 9 10 11 12
# Young Produced
�
'�
S
�(
a f
5
7
0 0
�
tim
SY
Adults Live / Dead
L
t—
I -I
L..
L
L_
L--
t--
L
L
I
r,...u� 1 0 4 d F R 7 R 9 10 11 12
# Young Produced
0
L
0
0 0
0
0
0
0 0
0
0
Adults Live / Dead
SY
Adults Live / Dead
L--
t—
I -I
L..
L
L_
L--
t--
1---
�-
I
Produced
Percent of Control producing third brood � %
Test Sample Organism Reproduction
Effluent %: cj.0
M— -a) 1 7 .1 4 5 6 7 8 9 10 11 12
# Young Produced
0
0
0
0 0
0
0
0
0 0
0
0
Adults Live / Dead
Adults Llve /Dead
l-
L
_ L-
L-
z--
r!
�
L—
r._.. sae 1 7 1? d F R 7 fS 9 1 u 11 14
# YounN Produced
3/!
(
Duration
Sample 1
Sample
1
hours
Sample 2
�
hours
Adults Llve /Dead
l-
L
L,
(✓-
�^
r!
�
�-
L
L
�-
(—
r,_.. u -i 1 7 11 d r, R 7 8 9 1 u 11 1 z
-w, ...
# Young Produced
100% pH
G / C?
Duration
Sample 1
Sample
Transfer Day
hours
Sample 2
9/
hours
I
Adults Live / Dead
l-
L
L
lr
l�
�--
C--
t^
ATotal Produced r `� �'
Comments
Batch #
100% pH
G / C?
Duration
Sample 1
Sample
Transfer Day
hours
Sample 2
5
hours
Batch #
/��
7
Sample
Sample 1
Sample
Transfer Day
0
2
5
Hardness
Spec Cond
(umhos/cm)
Chlorine
1
L0 /
(mg/L)
Rece;ptSampie
Temp
nN 1ctSamnle
Control�(�
�
7 Z
Sample
-.1— -.1
n_O_ 1st Sam ole
TPmn_ 1st Samnle
Control
�
7 Z
Control
Sample
,01
�6
TPmn_ 1st Samnle
Control
SampleU
2nd Sample
M7,77---- ma
2nd Sample
7, is 9iina
2nd Sample
Transferred by Fed by
Day 0 -e4 I-V
Day 1 L -t-1
Day 2 (A . _ Z -V
Day 3
Day 4 /VP -
Day 5 Z -V
Day 6 I-V
Terminated by _\
2nd Sample
Er
m; is 7—nal
2nd Sample
HR.'
nal
2nd Sample
�4 �

MERITECH, INC. M.Htech Sample ID 4'
Bioassay Sample Chain of Custody
642 Tamco Rd. Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike reedArneritech-labs.com_ Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client.rl ©--' /�k fi'� PO#.
Contact Person: Qf C,.At -s(rl%/�or!/'-L-^� NPDES#: NC f -Q ;25 q,5' +'S
Address:A96i2ei- Phone.
City: t, hywlf N'e2 Pipe #.
CountyaAJS State: -.ac— Zip- oZ�-5-7 k
SAMPLE INFORMATION
��e(/ //
Sample Site: , $i P-,,-,�n�-e,&k 1,
Sample Type: ❑ Grab Composite # of containers.
Sampling Time: Start Date: R StartTime: AM PM
End Date: %'-%� End Time: IO_'DD A PM
'°** Triple rinse sample container with sample before rifling. Completely fill the sample container with no airspace.
Pack the sample cooler completely in ice. The sample must be < 6.LrC upon receipt at the laboratory'
If r ` Q -./ Signature: � . ` r
Collector's Name: Print/ r`Il l 6. -"-t a s ry-1 is 0 9 ,
Test Required:L.10Chronic (7 days)
❑ Acute (24-48 hours)
IWC:
Comments:
TOXICITY TEST INFORMATION
Test Organism: �dsceriodaphnia dubia (water flea)
,52Lpjmephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (If multiple dilutions):
SHIPPING INFORMATION
Relinquished by.
Date Al Time: PM
Received by Date* �S Time. PM
r-
Relinquished b Date 3 Time. AM PM
Received by:� Date: Time AM PM
Relinquished by: Date: Time. AM PM
Received by Date: Time: AM PM
Relinquished by. Date: Time AM PM
Received by: Date: Time, AM PM
Sample Temperature CC):
Method of Shipment ❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑ Delivered ❑ Other
Samples shipped on Friday must be FedEx and must be dearly labeled for Saturday defivem NO SIGNATURE REQUIRED "
�% SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:- r�
Received Date: - ` ^` Time: 1310 AM PM
Sample Temperatures CC): / t, 3 / / Sample Condition , �
VWHITE = Laboratory copy YELLOW = Cliient copy

MERITECH, INC. Mentech Sample ID
Bloassay Sample Chain of Custody
- 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
NPDES#: NC OCz 1J5'�
Contact Person:
��� � Phone:
Address: f�7'� ? `!
City' S iCtiytrrl n% Pipe #:
Cit
County: .,t �s�+.s��/ _ State: AL C— Zip
SAMPLE INFORMATION
Sample Site: .
571-
Sample Type: ❑ Grab Composite # of containers'_
Sampling Time: Start Date:
�aZ fq- � o Start Time: f Q[s � PM
End Date. 5 �o End Time PM
*t` Triple rinse sample container with sample before filling. Completely fill the sample container udth no airy ce.
Pack the sample cooler completely in ice. The sample must be < 6 0°C upon receipt at the laboratory
A -
���% - P
Collector's Name: Print: t�."'t• Sj �"� S''� Signature:
TOXICITY TEST INFORMATION
Test Required: Jl Chronic (7 days) Test organism: p periodaphnia dubia (water flea)
❑ Acute (24-48 hours) j p1mephales promelas (fathead minnow)
El Mysidopsis bahia (shrimp)
Me: % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
r,...c Date: %Z_ /S'-/�4 Time: 1t aJ PM
Relinquished by: � l
/ - Time: ,
Time:A; J., PM
Received by Date: �— ti
I 3 AM PM
Relinquished by. f F
Date: _
' Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by.
Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by:
Date:
Time: AM PM
Received by.
Sample Temperature CC):
Method of Shipment:❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑Delivered [] Other
Samples stripped on FAY must be FedEx and mast be dearly labeledfor Sabnlft defterY, No SIGNATURE REQUIRED "
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by.
Received by: % 1
/ Date:
j l l f s l f c Time: 1, ,3 G AM OM
`
-1
Sample Condition:
Sample Temperatures CC):

Effluent Toxicity Statistical Results - Chronic Pass/Fail
Date: 12/21/16
Facility: CLAYTON WWTP
NPDES#: N00025453 Pipe#: 001 County:JOHNSTON
Laboratory Performing Test: MERITECH LABS, INC.
Reduction: -12.828
CONTROL 2.0% Effluent
# Replicates
12
12
Female Live
12
12
Adult Male
0
0
Adult Dead
0
0
Adult Mortality
0.00-w
0.00%
# Neonates
273
308
Mean # Neonates
22.750
25.667
Standard Deviation
3.251
1.826
Coefficient of Variation
14.290%
23
Fisher's Exact Test
A = 12
B = 12 a = 12 b = 12
a/A = 1.00 b/B = 1.00
Success is: survival
Critical b value = 8
12 > 8
The test concludes that the proportion of survival is not
significantly different for the control and the effluent groups.
Test Passes!
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA
i Group Neonates
1
C
15
2
C
18
3
E
23
4
E
23
5
E
24
6
E
24
7
C
22
8
E
25
9
C
23
10
C
23
11
C
23
12
C
23
ORDERED
OBSERVATIONS
Centered
i
Group
Neonates
Centered
-7.7500
13
E
26
26
0.3333
0.3333
-4.7500
14
15
E
C
24
1.2500
-2.6667
16
E
27
1.3333
-2.6667
-1.6667
17
E
27
1.3333
-1.6667
18
E
27
25
1.3333
2.2500
-0.7500
19
20
C
C
25
2.2500
-0.6667
0.2500
21
E
28
2.3333
0.2500
22
E
28
2.3333
0.2500
23
C
26
3.2500
0.2500
24
C
26
3.2500
J

SHAPIRO-WILK'S TEST FOR NORMAL, DISTRIBUTION OF DATA (cont.)
COEFFICIENTS AND DIFFERENCES
i x(n-i-1) x(i) a(i)
1
3.2500
-7.7500
0.4493
2
3.2500
-4.7500
0.3098
3
2.3333
-2.6667
0.2554
4
2.3333
-2.6667
0.2145
5
2.2500
-1.6667
0.1807
6
2.2500
-1.6667
0.1512
7
1.3333
-0.7500
0.1245
8
1.3333
-0.6667
0.0997
9
1.3333
0.2500
0.0764
10
1.2500
0.2500
0.0539
11
0.3333
0.2500
0.0321
12
0.3333
0.2500
0.0107
W =
X 136.1691
x(n-i-1) - x(i)
Calculated W = 0.890 Critical W = 0.884
0.890 a 0.884
The reproduction data is normally distributed
evaluated at a 99% confidence interval.
Test Passes!
F test for Homogeneity of Variance
Control variance 10.5682
F = _ = 3.17
Effluent variance 3.3333
11.0000
8.0000
5.0000
5.0000
3.9167
3.9167
2.0833
2.0000
1.0833
1.0000
0.0833
0.0833
Numerator degrees of freedom: 11 Denominator degrees of freedom: 11
Critical F = 5.32
3.17 s 5.32 =► The Test PASSES, the variances of the two groups
are significantly the same, homogeneous.

EQUAL VARIANCE t TEST
22.8 - 25.7
t = = -2.710
1.076
Degrees of freedom = 22
Critical t = 2.508
-2.710 < 2.508
Test passed. There is not a significant difference in reproduction
between the Control and the effluent evaluated at a 99°s confidence interval.
Chronic Test PASSES

_ - icffirient Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test
Facility' Gayton
Laboratory Meritech, Inc
X
in
NPDES # NC00 25453 Pipe #: 001
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Date•1212712016
county: Johnston
The 0 5% test concentration is not included in
the final result due to single statistical Inversion.
Test Initiation Datefrime 12/13/2016 2 12 PM Avg Wt/Surv. Control 0.853
% Eff. Repl.
1
2
3
4
FC-o-n-t-ro-11 Surviving #
10
10
10
10
Original #
10
10
10
10
Wt/original (mg)
0.811
0.918
0 853
0 831
0 5 Surviving #
9
10
10
10
Original #
10
10
10
10
Wt/original (mg)
0.431
0 676
0.663
0 631
o Surviving #
9
10 1
9
10
Original #
10
10 1
10
10
Wt/original (mg)
0 757
0.808
0 690 1
0 797
1=.J Surviving #
9
9
10
9
Original #
10
70
10
10
Wt/onginal (mg)
0 870
0 758 1
0.813 1
0 795
® Surviving #
10
10
10
10
Original #
10
10
10
10
Wt/original (mg)
0 851
0.797 1
0 856
0 749
l=.J Surviving #10
10
10
9
Original #
10
10
10
10
Wt/onginal (mg)
0.903
0 782
0.900
0 774
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) InitlFin
DO (mg/L) Init/Fin
Temp (C) [nit/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlodne(mg/L)
Temp. at Receipt (°C)
% Survivall 100 0
Avg Wt (mg)F__0_8_53_]
% Survival 97 5
Avg Wt (mg) F 0.600
% Survival 95.0
Avg Wt (mg) F___0_7_6_3___j
% Survival 92 5
Avg Wt (mg)F-0 809
% Survival 100 0
Avg Wt (mg)F__0__813
% Survival 97 5
Avg Wt (mg) 0 840
Day
n i 2 3 4 5 6
Test Organisms
F Cultured In -House
)r: Outside Supplier
Hatch Date' 12/12/16
Hatch Time 3.00 pm CT
8 09 / 7 79
8 05 / 7 75
8 07 / 7 60 18
05 / 8.08
8.22 / 7 79
S 10
/ 8 07
8 21 1 too
7.91 / 7.80
7.98 / 6 91
8 00 / 6.20 17
95 / 7.78
7 98 / 7 28 1
801
/ 7.33
821 / 658
25.6 / 25.8
24 9 / 24.7
24.4 / 24 5
124 7 / 24.9
24 6 / 24 7 124
5
/ 24 6
1 254 / 246
n 1 2 3 4 5 6
8.09 / 7 89
8 07 / 7.70
8.00 / 7.67
8 00 / 8.09
8 25 / 7 92
8 16
/ 8 00
8.16 / 7 78
7.81 / 6.92
7.92 / 6 67
7.91 / 6.54
8 03 / 7 78
8 07 / 7.36
8 02
/ 7.38
8 12 / 6 71
25.8 / 25 3
24.7 / 24 7
25.2 / 24.1
24 2 / 25 9
25.9 / 24 8
25 0
/ 25.3
24 9 / 24 9
1 2 3
12/12/2016
12/14/2016
12/15/2016
Normal
yes
FYI
228
23.4
228
70
71
72
91
88
88
473
648
660
<0.1
<0.1
<0.1
1.3
0.9
0.8
Dilution H2O Batch # E44
1104 1105 1106 1107
Hardness (mg/L) 42 44 44 50
Alkalinity (mg/L) 53 54 52Conductivity (umhos/cm) 192 205 188 209
DM Foran AT -5 (1/04)
Overall Result
ChV >8
Stats
Survival
Growth
Normal
yes
FYI
Hom Var
p'"j
lel
NOEC
8
8
LOEC
>8
>8
ChV
>8
>8
Method
Steel's
Dunnett's
Overall Result
ChV >8
Stats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
0.5
See Comment
See Comment
1
10 14
2.36 2.3437
2
10 12
236 1 1491
4
10 18
2.36 1.0387
8
10 16
236 03506

Client: Clayton
NPDES #: 0025453
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber: 10
Test Vessel Size- 400 mi
Initiated by: /,q K Nest Solution Volume: 250 ml
tv 13-13-I.
Date/Time Fed: 12/13/16 I'?Uev-lTemp. of Stock: 2� °C
Date/Time Born: 12/12/16 3:00 PM CT
Organism Source. Aquatox, Inc.
Transferred by:
Date
Time Initials
Day 1 _L2/14
Initials
Day 12/15-l�nl'j�Z-V
Day 0
Day 312/16
-09/Z-1/
4 12/ 17
"j �U �``
Day
•
Day 5
Nit,-
_12/183
Day 12/19
C iivx
# of Reps- 4
Analyst(s), MR, CD, LV, SB
Start Date: 12/13/16 Time:
End Date: 12/20/16 Time-
Randomization- G
No
Incubator #'
Fed by:
Test Termination Data:
Initials/Signature:LV// ti/4'"014
End Date: 12/20/16
Date
Initials
Time 2 Initials
Day 0
12/13
�aTime/1
-1 , �V4p�
1 k
Day 1
12/14
Z-Ul/
Day 2
12/15
'-� ,�} M✓i�
L
Day 3
12/16
-( dam.
Day
12/17
NUS �����7
L✓
Day 5
12/18
Day 6
12/ 19y!�
Test Termination Data:
Initials/Signature:LV// ti/4'"014
End Date: 12/20/16

ENVIRONMENTAL LABORATORIES
A Division of [Nater Technology and Controls, !nc_
Chemical and Physical Determinations
Client: Qa on
NPDES#: 0025453
Start Date:
12/13/16
12/20/16
Test Organism: PimepWes
promelas
End
Date:
Concentration: control
0-1
1.2
2-3
Day
3-4
4-5
5-6
6-7 Remarks
Soft Synthetic Freshwate l I
/O I I G��
V I
Batch # .
pH: Initial %
7
2Z:27
�tSCP
Final
,.-
• v ► 7,71 1
'
,U
_Z/1�.
D.O.: Initial
Final '�, d A
! g
: A
Temp. Initial
�FinalConductivity:InitialFinal
M�'a
Residual Chlorine: Z .1
G 1
LU .
I
G v
G0 f
Z�6 '
Hardness:
-
Alkalinity.
r7 7
2
7
y
Concentration: 0.50%
0-1
1-2
2-3
Day
3-4
4-5
5-6
j�
6-7 Remarks
! -7
pH: Initial
Final
7-_55 ��
()
? ,
D.O.: Initial 7,
Final
7, id_
Temp.: Initial
;)�
Final
Conductivity: Initial
S LP
20
- L
Final
j
�''
` -15
Residual Chlorine:
ted.I
LO, I
L b. I
LG. I
• j
G• I
L Q" 1
0
Concentration: 1.00%
0-1
1-2
2-3
Da
3-4
4-5
5-6
6-7_ Remarks
pH: Initial
_
l Final
7- XO
—7
7 �
'7
�a
D.O.: Initial
Final
Temp.: InitialO�
Final
5,
.1/
.�
��r
a�
•a I l
j Conductivity: Initial
(3
?moiA(P
Final
Residual Chlorine:
G U. (
j D l
LPA 1
G Q•
/-0 -
j
, f

•
MERlTE�'H•. //�C`..
ENVIRONMENTAL LABO � co0 R`ES
a orv� re.. or w+rac rocr,.,erovr �.^ r
Chemical and Physical Determinations Page 2 of 2
Client: Cla on NPDES#: 0025453 Start Date: 12/13/16
Test Organism: Pimephales promelas End Date: 12/20/16
Concentration:
2.0%
0-1
1-2
2-3
—
3-4
4-5
5-6
61I-7 Remarks
pH:
Initial
. 0-6
5", 0
,S a 7
g
��
Residual Chlorine: -f- ��,
Final
Final
-7- $
—7_
n
% - z
r� o2
C.
D.O.:
Initial
7
7-q
)1 16
7-c.
7,36
•'
Final
Final
G . ci �
4' i 0�
c? -J;
r
rr
�? J
Temp.:
Initial
5 3
S r`i
-S
;���
•y
�M•
��
Final
Final
�S
�� -
'
A IS
7 - q
�J .
Y, 7
Conductivity:
Initial
q 1-7
-30
�`� o�
��
GLa�
�ai
�S t-
Final
Final
L
. /
2-0 - I
5:3
9"3
Residual Chlorine:
Residual Chlorine:
Z-5 t
Z-0. I
",I I
c D
I LQ'
Concentration:
4.0%
0-1
1-2
2-3
3-4
4-5
5-6
6-7 Remarks
pH:
Initial�-
��j
�'• i� G'i
i-- Q
g
��
Residual Chlorine: -f- ��,
Final
7, �r2
�.7
q
_
�l , 0
7� 2G
�`%
�i✓1
D.O.
Initial
(p
, 2 7
f'
)1 16
7-c.
7,36
S
Final
l.��
(; '1
4' i 0�
c? -J;
-3v
�? J
Temp.:
Initial
Final
5 3
`�r-j.
-Z)
`���
` '�-
55 _ h
55
��
Final
Li.
�S
�� -
u ' 0
a
7 - q
�J .
Conductivity:
Initial
r a`j
}
-30
�`� o�
��
GLa�
�ai
Final
v
L
. /
2-0 - I
Gd
Residual Chlorine:
ZD, I
Z-5 t
—z -o.,
LQ r
z -o r)
z-0-1
I LQ'
Concentration:
8.0%
0-1
1-2
2-3
3-4
4-5
5-6
6-7 Remarks
pH:
Initial
Final
.d�
,v;
i-- Q
Residual Chlorine: -f- ��,
D.O.:
Initial
� . $ l
% `� �
cL
Q7-`t'st
`L�5L
19
Final
6-,-qta
6,-
-64
7-c.
7,36
S
Temp.:
Initial
�j
.�
c? -J;
? q-5
LI
Final
5 3
`�r-j.
9-6, `72,4
•
a5
��
Conductivity:
Initial
-9,L10
a
_-,I Ll�?
1
Final
?
Residual Chlorine:
zQ. f
L
. /
2-0 - I
z-0
Gd
ria V
Concentration: 100% 0-1
1-2
2-3 1
3-4
4-5
5-6
6-7
Remarks
pH:
Conductivity:
Residual Chlorine: -f- ��,
z -cc
Hardness: 1q0
Alkalinity:

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Chent: Clayton
Test organism: Pimephales promelas
Concentration Control
NPDES#: 0025453
Start Date- 12/13/16
End Date- 12/20116
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 __S.—_ __L_ --�
Rep B 0
_ v - -- 9
Rep C 0
L/
Rep D 0— -
Concentration 0.50% /,L 1.2-fe/6
Tuesday Wednesday Thursday Friday Saturday 'bunday Monday Tuesday
Rep A 0 —L.L- ---�-- �1---— L/
Rep B 0
l �
Rep C 0
Rep D 0 —.LL
Concentration 1.00%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
Rep B
Rep C 0
Rep D 0
�2

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Cla on
Test Organism: Pimephales promelas
Concentration 2.0%
NPDES#: 0025453 Start Date: 12113116
End Date- 12120116
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A —0
Rep B 0-
Rep C
Rep D
Concentration 4.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
,�l 0
Rep A 0 � ----L — (—)v
Rep B 0
Rep C
Rep D 0 _o
Concentration 8.0%
Tuesday Wednesday Thursday Friday Sa urday Sunday Monday Tuesday
r
Rep A 0 —�`—
Rep B 0
Rep C
0
Rep D 0
b o o

�Cp, ® ,,
?' ENVIRONMENTAL LABORATORIES
q Orvlsion orlRater Technology -nd controls !nc
FISH LARVAL SURVIVALIGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Page: 1 of 2
Facility: CI_�n
Outfall:__
NPDES #: 0025453
Date of Test: 12/13/16
Initial weights taken on: 1 �'2 b L-✓ Organism: Pimephales promelas
V Y'
Final weights taken on: % -� -% by: LL
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
F ORG.
WT. OF
(A 9)
# ORG.
N
WT.IMEAN
(mg)
SURV
CONC
REP
10
.7r
U
Ci
01
(.J t J�r�C.► r�
D 05
Rd
l
/ }
moi_ as
X155 3
�, > 1
LQ 1
3
A
�
0,676
_\
0.50%
c�; I0�
1G.
�
�n 6
�L�
C•� �
�L��
D
v g6,
-7,5 7
A
o8
�a
0, >o E
1.0%
6.qo0,,
-Ll-�D
6 q
g3a.
7 q7
110
o-�-?
100

Facility: Clayton
NPDES #: 0025453
AE.--®THC'r Hct:
ENVIRONMENTAL LABORATORIES
A Dwsion or Water Technology and Controls, Inc
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Date of Test: 12/13/16
Initial weights taken on: 0'" by: L -V
Final weights taken on: o1- a.1-16 by: f�1/
Page: 2 of 2
Outfall:
Organism: Pimephales promelas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV %
A
17 36
P
--7
2.0%
aD
213
1 DD
4.0%
A
?17.91-(
b S l
10
(M)51
/0
B
8()gl5(p
��
7q�
`�
0' 7q
D
RaI-- 7
936.03
10
67
106
D
�15- Y
�����
7L,
10
IN
�a
°
8.0%
A
Wa3.0�23
d,67
q.
It)
o. qo3
too
B
�i ,-7521q5-7
7 9Q,D
�r
0o
C
SIV6
8Q . l(
'-00
j
D.100
100
D
R 1. 205
2 ,�?a zq
-771 /
0 - / /
W

MERITECH, INC. Meritech Sample ID#:
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
P1lone. 1-336-342-4745 Fax: [phone]
Toxicity Supervisor email: mike reedp_meritech-labs.com Web Site- www.meritech-labs.com
CLIENT INFORMATION
Client
.rf ©T' /moi
PM
PO#:
`�� C
Contact Person:
Cl✓i^��
Time,
NPDES# NC�Q e�3
PM
R,,1�
AM
Phone:
Address:
AM
PM
Pipe #.
City.
�' ill %�-�
State. Zip:
Countyti
o -J5
-1���
SAMPLE INFORMATION
pn )
� it �%L�J'r %
Sample Site:
/,I !i (��iti/L L.✓
Sample Type:
❑ Grab .Composite
Mof containers:
Sampling Time: Start Date:'F. AQ4!'i % Z—jZ-* StartTime: �1 •'ID AM PM
End Date: %7
End -rime:
A PM
—T rinse sample container with sample before filling. Completely fille sample coiner with no air space_
Pack the sample Cooler completely lin ice. The
sample must be < 6 ll°C upon receipt alaboratory—
Collector's Name: Print/,,/r��llrtt�'i Jc �r�+'I/ p Signature
Test Required: Chronic n days)
❑ Acute (24-48 hours) _
IWC:
Comments:
SHIPPING INFORMATION
Relinquished by.
Date:
Received by Date: J /
Relinquished b
Date- �j J5 G •�
a
Received by sf Date:
Relinquished by. Date.
Received by. Date:
Relinquished by: Date:
Received by. Date:
Sample Temperature CC):
TOXICITY TEST INFORMATION
Test Organism: f &edodaphnfa dubia (water flea)
- -- ____5ZBmepha1es promelas Cfathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions)
Time /0: a -
r ?-01
PM
Time 1/1'.' � PM
I yIG7
Time: r—
v
AM
PM
Time
AM
PM
Time,
AM
PM
Time.
AM
PM
Time:
AM
PM
Time:
AM
PM
Method of Shipment, F-1 UPS ❑ Fed EX ❑ Meritech Pick-up ❑ Delivered ❑ Other
'* Samples shipped on Friday must be FedEx and must be dearly labeled for Sd wday delaery, NO SIGNATURE REQUIRED "
1% SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: � R 4L
Received !--- Date: 1 d - � + �` Time: 1310 AM
Sample Temperatures CC): �: J / rt 3 i ! Sample Condition:
WHITE = Laboratory copy YELLOW= Ment copy

MERITECH, INC. Merltech Sample ID
Bioassay Sample Chain of Custody
AA
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax.[phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client* :X�D 0 Cl q *2'Al PO#:
y NPDES#: NC (JOi ir 415 S
Contact Person: � �� ��f �S �Li//'^e. �''1 - -
Address* M622i ?�1 Phone: /9-fSJ--. *
City: 1 An, Vfty '� Pipe #-
County: State: �� ZiP=�2
SAMPLE INFORMATION
Sample Site: L
c L L✓ Gk �� �(�
Sample Type: ❑ Grab Vzomposite # of containers,
Sampling Time: Start Date. 1.2 /{, Start Time: 1"J/3— AM PM
End Date:/ 1 End Time: �l� AM PM
*" Triple rinse sample container with sample before filling. Completely Fill the sample container with no airspace
Pack the sample cooler completely in ice. The sample must be < 60C upon receipt at the laboratory"`
Q0 /°4 /J r
Collector's Name: Print: /�/� f (,`r,, �C f�`.K -� Signature_ }!
Test Required: Chronic (7 days)
❑ Acute (24-48 hours)
(WC: %
TOXICITY TEST INFORMATION
Test Organism: RCeriodaphnia dubia (water flea)
E2 Pimephales promelas (fathead minnow)
❑ Mysidopsis Bahia (shrimp)
Test Concentrations (if multiple dilutions)
Comments:
SHIPPING INFORMATION
Date, J7 -15"-1k
Time. 160-5—
PM
Relinquished by.
Received by
Date: -
Time: )v S
7�
A
PM
Date: G
Time.
AM
PM
Relinquished by
Received by
Date.
'rime:
AM
PM
Relinquished by.
Date:
Time'
AM
PM
Received by:
Date-
Time:
ANI
PM
Relinquished by.
Date:
Time.
AM
PM
Received by:
Date:
Time:
AM
PM
Sample Temperature (°C):
Method of Shipment❑
UPS ❑ Fed EX ❑ Meritech Pick-up ❑ Delivered
❑ Other
Samples shipped on Friday must be FedEx and must be dearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
`•
SAMPLE RECENING (Laboratory Use Only)
Relinquished by:
Received by:
Sample Temperatures CC): 0 j
Date. ) J Time: AM PM
Sample Condition:
WHITE = Laboratory copy YELLOW = C19ent copy

MERITECH, INC. Mentecn sample 10
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: i-336-342-1522
Toxiclty Supervisor email: mike reedCalimeritech-labs.com Web Site. www.meritech-labs.com
CLIENT INFORMATION
1
Client:.�.��� �l4 L� �. c✓� Pot�11i�� �� .'
Contact Person: „u�vt.d , % % s�y t .�% NPDES#: NC QD-.JV3 3
Address. p� 3 D f 87 s Phone:
City: n/4 YT Pipe M E%p�
County. State: Zip:
n nSAMPLE INFORMATION G�
Sample Site: % • / / �� CY��� �ffC /' ���c%��r*J/
Sample Type: ❑ Grab Composite # of containers'_
Sampling Time: Start Date: jz-/,y �/ Start Time: 'L10 AM PM
End Date: /� '%(� `�t'it End Time"PM
*** Triple rinse sample container with sample before filling. Completely rill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory"*
Collector's Name: Print• %,%, 1/114 S/7�tpf4"� Signature
TOXICITY TEST INFORMATION
Test Required: �4hronic (7 days) Test Organism: �5Xeriodaphnia dubia (water flea)
❑ Acute (24-48 hours) `f9f—Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC; % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION/
/ �� i %
Time:
10 ''1 57
AM PM
Relinquished by: Ni^2' .•
�
Date:
% 2 /
Time.
�.
PM
Received by: �- �
r ,
Date:
Date.
)
( ��
� 3
Time.�
l�
AM PM
Relinquished br. — //
AM PM
Received by;/
Date:
Time,
Date:
Time*
AM PM
Relinquished by:
Time
AM PM
Received by:
Date
Date.
Time.
AM PM
Relinquished by
Time:
AM PM
Received by'
Date'
Sample Temperature (°C):
Method of Shipment:❑ UPS ❑ Fed FSC 0 Meritech Pick-up ❑ Delivered ❑ Other
Samples shipped on Friday must be FedEx and must be dearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
Relinquished by:
Received by: ` 7 I1 Date: 1 i 1, Time: O Alk - PM
Sample Temperatures (°C)' G �5 / / Sample Condition: i C'
WHITE = Laboratory copy YELLOW = Client copy

Title: clayton - survival Transform: No TRANSFORMATION
File: claysury
Shapiro - Wilk's Test for Normality
--------------- ------------------------------------------------------------
D = 0.0350
W = 0.9485
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

T
•Title: clayton - survival
File: claysury Transform: NO TRANSFORMATION
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
--------------------------------------------------------------------------
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.

i
Title:
clayton - survival
Transform:
NO
TRANSFORMATION
File:
claysury
Steel's Many -One
Rank Test -------Ho_
Control<Treatment
----
------------------------------------------------
--
MEAN IN
RANK
CRIT.
SIG
GROUP
IDENTIFICATION
ORIGINAL UNITS
-----------
SUM
-------
VALUE
-DF-- 0_05
1
control
1.0000
0.9500
14.00
10.00
4.00
2
,5
1
0.9500
14.00
10.00
4.00
3
2
0.9250
12.00
10.00
4.00
4
j
4
1.0000
18.00
10.00
4.00
5
58
0.9750
16.00
10.00
4.00
------
I Critical values are 1 tailed ( k = 5 )

Title: clayton
File: clayton Transform: NO TRANSFORMATION
Kolmogorov Test for Normality
----------------------------------------------------------------------
D = 0.1589 (p -value > 0.100)
D* = 0.7391
Critical D* = 1.035 (alpha = 0.01 , N = 20)
= 0.895 (alpha = 0.05 , N = 20)
----------------------------------------------------------------------------
Data PASS normality test (alpha = 0.01). Continue analysis.

a - -
Title: clayton
File: clayton Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
--------------------------------------------------
Calculated B1 statistic = 0.7345 (p -value = 0.9470)
Data -PASS B1 homogeneity test at 0.01 level. Continue analysis.
----------------------------------------------------------------------
Critical B = 13.2767 (alpha = 0.01, df = 4)
= 9.4877 (alpha = 0.05, df = 4)

_ Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 10/06/16
Facility: CLAYTON WWTP NPDES#: N00025453 Pipe#: 001 County: JOHNSTON
Labo Perf 6rming Test. MERITECH LABS, INC.
Comments
Carae
IX
Signure o -Laborator * PASSED: -6.618 Reduction
Work Order: Environmental Sciences Branch
MAIL ORIGINAL TO: Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
7orth Carolina Cerioaapnnia
Chronic Pass/Fail Reproduction Toxicity Test
,ONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced 1116119120123119120121118116118120117
Adult (Wive (D)ead JAL IL IL IL IL IL IL IL IL IL IL IL
affluent g: 2%-
'REATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced 1120119123119119121121120119121121119
Adult Wive (D)ead IIL IL IL IL IL IL IL IL IL IL IL IL
Chronic Test Results
Calculated t = -1.787
Tabular t = 2.508
t Reduction = -6.61
!k Mortality
Avg.Reprod.
0.00
18.92
Control
Control
0.00
20.17
Treatment 2
Treatment 2
Control CV
10.9178
PASS FAIL
g control orgs
X
producing 3rd
High
n.,,,
brood
Check One
100
1st sample 1st sample 2nd sample Complete This For Either Test
i pH Test Start Date: 09/28/16
Control 8.10 6.72 8.15 8.08 8.12 8.11 Collection (Start) Date
Sample 1: 09/26/16 Sample 2: 09/28/16
Treatment 2 8.08 8.13 8.02 8.08 8.14 8.08 Sample Type/Duration 2nd
1st P/F
f s s s Grab Comp. Duration D
t e t e t e I S S
a n a n a n Sample 1 X 23 hrs L A A
r d r d r d U M M
t t t Sample 2 X 23.8 hrs T P P
1st sample 1st sample 2nd sample
D.O. Hardness (mg/1) 48 ........ .........
Control 7.30 7.79 7.96 7.50 7.91 7.40 .
Spec. Cond. (�.unhos) 166 577 619
I Treatment 2 7.16 7.82 8.00 7.30 7.85 7.42
Chlorine (mg/1) ........ <0.1 <0.1
i LC50/Acute Toxicity Test Sample temp. at receipt(OC) 1.4 1.1
(Mortality expressed as %, combining replicates)
Note: Please
Concentration Complete This
Section Also
Mortality
start/end start/end
I,C50 = t Method of Determination
95* Confidence LLimits Moving Average_ Probit _
-- o Spearman Karber _ Other
Control
t
!k
-t
High
n.,,,
%
Note: Please
Concentration Complete This
Section Also
Mortality
start/end start/end
I,C50 = t Method of Determination
95* Confidence LLimits Moving Average_ Probit _
-- o Spearman Karber _ Other
PH
Organism Tested: Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT -1 (3/87) rev. 11/95 (DUBIA ver. 4.41)
o •
Control
High
n.,,,
PH
Organism Tested: Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT -1 (3/87) rev. 11/95 (DUBIA ver. 4.41)
o •

Meritech, Inc.
I
Mini Chronic Pass/Fail Test: Ceriodaphnia dubia Incubator #:
Client: 1G Pipe #:_) County: -� Date Start: Cf--( Date End:
NPDES #: N K Date /Time of Culture Transfer: -� -101., Time Start: to, Y Ot ,q,°n Time End: "�
Dilution Water: Lake Brandt Date / Time Neonates born:, )., �j.f1J' 1 st Renewal Date: —� ,
,4LM� �_3n-6r,— Time:
Test Organism Source: Tray # A e of eonates at Test Start:ot hours 2nd Renewal Date: Q ��J Time: _C M
Y g
Stirred / Aerated for D -0.: .Y /(9 Randomized: Y / N Culture Tray Temp: 2:n -2_1c Analyst(s): MR, Co,,SC;�R LW 68 Reviewed by: ,kj>L
r%.., 4ilg 1 / i d ri A 7 Q r% A A A A
#Young Produced
0
0
1 0
0
0
0
0
0
0
1 0
1 0
0
Adults Live/ Dead
�.-
1-
'%-
C1
L
C
-.-
�I
U--
Ik-
t;-
%,-
n.r$1i 1 . 2 l_ 4 _89 . fi 7 Q n Art AA An
n -al $7 1 Z .14 d -8; A 7 a n AA AA
# YoungProduced
Uri ire©,ave©
0
-
0
j
—
0
0
0
0
raW"arc
Iv
0
MMMMmmm
1G
Adults Live / Dead
�.-
Spec. Cond.
(umhoskm)
n -al $7 1 Z .14 d -8; A 7 a n AA AA
# YoungProduced
0
0
-
0
j
—
0
0
0
0
v
0
Iv
0
1 I
1G
Adults Live / Dead
�.-
Spec. Cond.
(umhoskm)
L
L—
t-
t
U--
i..
L
L
Total Produced1 v I -2-j 1 1 /117
Percent of Control producing third brood: t�U%
Test Sample Organism Reproduction
Effluent %:
n7u ft? 1 2 3 4 .r, R 7 A n .1A AA An
# Young Produced
0
0
-
0
0
—
0
0
0
0
v
0
Iv
0
II
0
IG
0
Adults Live / Dead
LL
Spec. Cond.
(umhoskm)
L—
5 7.7
Chlorines
(mg/L)
r`
Dav_#5 1 2 3 4. 5, 6. 7, 891n 11 19
# Yo
Adult
nnitE7 1 2 3 4 1; A 7 A n An AA An
,. � .. - •
Ili>������������
GIC?
Duration
Sam le 1
r
C -23,?
hours
hours
Sam le 2
' 7,
Hardness
l {�
UVA,
6�.,IN
Spec. Cond.
(umhoskm)
��
5 7.7
Chlorines
(mg/L)
• Total Produced
Comments:
Sample 1: - -I Sample 2:47--?Zzz—
Sample Information
Batch #
100% PH
GIC?
Duration
Sam le 1
r
C -23,?
hours
hours
Sam le 2
' 7,
Batch #
/
2q
4
Sample 1
Sample 2
Transfer Day
0 2
5
Hardness
l {�
UVA,
6�.,IN
Spec. Cond.
(umhoskm)
��
5 7.7
Chlorines
(mg/L)
Recelpt SampleI
Temp (°C)
nH 1at-Q-1.
Control
Sample
�,Oq
7
Y anal nnal
D.D. let Ramnta
Control
Sample
kb
7
Temp. 1st Sample
Control ; �,
Camnla n%A
2nd Sample
2,1 ��U
o �A
ImUafinal
2nd Sample
7,% b5
7°3o
Inlle Ina
2nd Samplleb
m
2tl "7
Transferred by. Fed by.
Day 0 Ida JL k/
Day 1
Day 2 14L- Lltii
Day 3
Day 4 L.I✓
Day 5 L W
Day 6 LAI%
Terminated by:
2nd Sample
�.l jl
,rlly �10�
Initis Ma
2nd Sample
45
In Tarnal
2nd Sample
?quk,..

V
MERITECH, INC. Meritech Sample ID #:—Gala1 U
Bioassay Sample Chain of Custody
- 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike reedOmeritech4abs.com_ Web Site: www.meritech4abs.com
CLIENT INFORMATION
Client: / t a.41 f1r r
AM
Po#
,%
Contact Person:
C��c r.�-G��
PM
NPDES#: NC Or?.�gS3
AM
AV 9
Time:
Phone: 41 g'Sf3` /S'3�S
Address:
1601- V�i`
AM
PM
City:
e J4. I17rZJ n1
Pipe #:
County:
State: ��
zip:
SAMPLE INFORMATION
Sample Site:
l-. j j ") G C,ndt;t 1-,,40rP
Sample Type:
❑ Grab mcomposite # of containers:
Sampling Time:
Start Date' 9 4 -1 r0 Start Time: 1010
A©
PM
End Date: 9 -Z z - m, End Time: Q.,10
AM
PM
'** Triple rinse sample container with sample before filling. Completely rill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0*C upon receipt at the laboratory'**
Collector's Name: Print: f 11,6tM JZ S; ins iJS &--/ Signature:
Test Required: >�Chronic (7 days)
❑ Acute (24-48 hours)
)WC: CR, 0 %
Comments:
TOXICITY TEST INFORMATION
Test Organism: 2�LCeriodaphnia dubla (water flea)
[�LPlmephales promelas (fathead minnow)
❑ Mysidopsis bahla (shrimp)
Test Concentrations (if multiple dilutions):
Relinquished by Date•
Received by. Date: -
Relinquished by Date: V
Received by: - Date:
Relinquished by:
Received by.
Relinquished by:
Received by.
Date:
Date -
Date:
Date:
Sample Temperature CC):
Time: " ► PM
Time: i G PM
Time: Iy AM PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Method of Shipment:❑ UPS ❑ Fed EX ;E�,11Aeritech Pick-up Delivered ❑ Other
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED'*
I SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: �-laj-
�(� J -
Received by: 7ImB
s (- — Date: � � : AM PM
Sample Temperatures CC): � + � 1 j_ 1 / Sample Condition:
WHITE = Laboratory coov YELLOW = Client cony

MERITECH, INC. MentechSample ID#: (A a01
' Bioassay SamAle Ch
aln of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike reedCa-meritech-labs.com Web Site: www.meritech labs.com
CLIENT INFORMATION
j 0 f /' f�.�tt�� POO
Client: � 1it.J J
Contact Person, ��� �.¢,S r��f4v C.-�e% NPDES#: NC dA0A6ri'S 3
Address: F'LO,,(�[//5'i5' Phone: 9/
City: Pipe P.
County: iAIS »V z.-/ State: Zip: 991'yS.z,Y
SAMPLE (INFORMATION p
Sample Site: AG ��- � y� t ,G %� L 77' 5Af
SampleType- ❑ Grab t9composite # of containers: a,
% o-130
Sampling Time: Start Date- Start Timer PM
End Date: 07 -�-! — / End Time. AM PM
*** Triple rinse sample container with sample before filling. Completely rill the sample container with no airspace.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory*
Collector's Name: Print &I,%hi ^ /� S;r•r/�So.✓ Signature:
TOXICITY TEST INFORMATION
Test Required: PI -Chronic (7 days) Test Organism: ,jPC,.erlodaphnla dubla (water flea)
El Acute (24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis bahla (shrimp)
IWC: j % Test Concentrations (if multiple dilutions):
Comments:
o SHIPPING INFORMATION
Relinquished by „ t9 Date: — g — rime:
PM
Received by Date: lU Time: ��
Relinquished by: Date: b2 _ Time: 0 AM PC)
Received by: Date: Time: AM PM
Relinquished by. Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by, Date: Time: AM PM
Sample Temperature (°C):
Method of Shipment ❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑ Delivered ❑ Other
" Samples shipped on Friday must be FedEx and must be dearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLE RECEMNG (Laboratory Use Only)
Relinquished by
Ll
Received by: Date: / J `Q Time: r t AM
Sample Temperatures (°C): I / I Sample Condition: 1
WHITE = Laboratory coov YELLOW = Client cenv

SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA. (cont.)
COEFFICIENTS AND DIFFERENCES
x(i) a(i) x(n-i-1) - x(i)
1
4.0833
-2.9167
0.4493
7.0000
2
2.8333
-2.9167
0.3098
5.7500
3
2.0833
-1.9167
0.2554
4.0000
4
1.0833
-1.1667
0.2145
2.2500
5
1.0833
-1.1667
0.1807
2.2500
6
1.0833
-1.1667
0.1512
2.2500
7
0.8333
-1.1667
0.1245
2.0000
8
0.8333
-1.1667
0.0997
2.0000
9
0.8333
-0.9167
0.0764
1.7500
10
0.8333
-0.9167
0.0539
1.7500
11
0.0833
-0.1667
0.0321
0.2500
12
0.0833
-0.1667
0.0107
0.2500
1
W = X 61.8515
64.5833
Calculated W = 0.958 Critical W = 0.884
0.958 z 0.884
The reproduction data is normally distributed
evaluated at a 99W confidence interval.
Test Passes!
F test for Homogeneity of Variance
Control variance 4.2652
F = _ = 2.66
Effluent variance 1.6061
Numerator degrees of freedom: 11 Denominator degrees of freedom: 11
r
Critical F = 5.32
2.66 s 5.32 =► The Test PASSES, the variances of the two groups
are significantly the same, homogeneous.

EQUAL VARIANCE t TEST
18.9 - 20.2
t = = -1.787
0.699
Degrees of freedom = 22
Critical t = 2.508
-1.787 < 2.508
Test passed. There is not a significant difference in reproduction
between the Control and the effluent evaluated at a 999.- confidence interval.
Chronic Test PASSES

e Irffluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date.10/6/2016
Facility. Clayton
X
S�gr�tnr� of Operator in
X
Signature of Laboratory
NPDES # NC00 25453
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Pipe #. 001 County: Johnston
Test Initiation Datemme
9/27/2016
2
2.42pm
4
Avg Wt/Surv. Control 0.848
Test Organisms
% Eff. Repl.
1
2
3
4
815 1781
Cultured In -House
FC-ont-r-o-11 Surviving #
10
10
10
10
% Survival100.0
� Outside Supplier
Original #
10
10
10
10
/ 2461246
/ 240.
Wt/original (mg)
0 709
0.963
0.767
0 953
Avg Wt (mg) 0.848
Hatch Date. 9/26/16
F_0_5-1 Surviving #
10
9
10
10
% Survival 97 5
Hatch Time. 3:00 pm CT
Original #
10
10
10
10
Wt/onginal (mg)
0.581
0.682
0.841
0.877
Avg Wt (mg) 0.745
0 Surviving #
10
10
10
10
%Survival 100.0
Original #
10
10
10
10
Wt/original (mg)
0 760
0.752
0.702
0.774
Avg Wt (mg) 0.747
0 Surviving #
10
9
101
10
% Survival 97.5
Original #
10
10
10
10
Wt/original (mg)
0 822
0.615
0 630
0 837
Avg Wt (mg) 0 726
® Surviving #
10
10
10
10
% Survival 100 0
Original #
10
10
10
10
Wt/original (mg)
0.913
0 705
0.962
0 927
Avg Wt (mg) 0 877
® Surviving #
10
10
lo
% Survival 100.0
Original #
10
10
10
10
Wt/onginal (mg)
0 875
0.764
0.911
0 813
Avg Wt (mg) 0.841
Water Quality Data
Day
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp. at Receipt (°C)
0
1
2
3
4
5
6
8.11 / 7.74
8.20 / 783
8.11 17.80
8.18 / 801
828 1797
815 1781
825 17.58
7.67 / 6.58
7 84 / 694
7.93 / 6.631794
/ 7.141769
/ 726 1807
/ 661
1771 / 5.69
24.4 / 25 3
24.0 / 24.9
24.1 / 241 1245
/ 25.21241
/ 2581248
/ 2461246
/ 240.
0 1 2 3 4 5 6
8.02 / 7.69 8 07 / 7.78 8 12 / 7.74 8.18 18.00 18 20 / 7.93 8.14 / TV 8 15 / 7.64
7.67 / 6.45 7.87 / 6 52 7.91 / 6.18 7.92 / 7_161 7.66 / 7 31 8.11 / 6 58 7.70 / 5.85
25.4 / 24.9 24.7 / 24.7 24.3 / 25.2 24 8 / 24.1 124.3 / 24.1 24.7 / 24.4 24 7 / 25 3
1 2 3
9/26/2016
9/28/2016
9/29/2016
Normal
0_1
no
230
23.8
244
78
74
74
73
72
69
640 1
619 1
564
<0.1
<0.1
<01
1.4
1.1
1.1
Dilution H2O Batch # 1075 1076 1077
Hardness (mg/L) 44 42 42
Alkalinity (mg/L) 56 57 56
Conductivity (umhos/cm) 204 208 193
Overall Result
ChV >8
Stats
Survival
Growth
Normal
0_1
no
Horn. Var.
F')
no
NOEL
8
8
LOEC
>8
>8
ChV
>8
>8
Method
Steel's
Steel's
Overall Result
ChV >8
Stats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
05
10 16
10 140
1
10 18
10 14.0
2
10 16
10 14.0
4
10 18
10 17.0
8
10 18
10 19.0

Client. Clayton
NPDES #: 0025453
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber: 10
Test Vessel Size: 400 ml
Initiated by: Z W,,1A K Test Solution Volume: 250 ml
Date/Time Fed: 9/27/16 10"000.11,'l Temp. of Stock: D " "-f °C
Date/Time Born: 9/26/16 3:00 PM qt # of Reps: 4
Organism Source: Aquatox, Inc, Analyst(s): MR, CD, LW, S !�,
Transferred by:
Start Date. 9/27/16 Time:
End Date. 10/4/16 Time,
Randomization: Yes No
Incubator #:
Fed by:
Date
Time
Initials
Day 1
9/28
9/27
)(�'C�(7.n�=Q
Day 2
9/29_��
Day 1
L+.✓�jj
Day 3
9/30
6 ,R5 Afn S`f3%Z�.✓
Day 4
10/1
Ri'1�41N
SG,,,
Day'5
10/2
O•DS�ym""
/
Day 6
10/3
. �j3
L, ! V
Start Date. 9/27/16 Time:
End Date. 10/4/16 Time,
Randomization: Yes No
Incubator #:
Fed by:
Test Termination Data:
Initials/Signature: 46V/
End Date* 10/4/16
End Time: 12 : %4 h::�
Date
Time 1 Initials
Time 2
Initials
Day
9/27
)(�'C�(7.n�=Q
�I,✓
Day 1
9/28
;/Sam (3
3 25�r►,
S�
Day 2
9/29
-' o 6k -"- S�
4-1:1DPWI
`�7�—'�`
� �✓
Day 3
9/30
i� `—""
L
Day 4
10/1
Day 5
10/2
Day 6
10/3
�` —
9*.3�'P
S'�'
Test Termination Data:
Initials/Signature: 46V/
End Date* 10/4/16
End Time: 12 : %4 h::�

• � it iitr i �� ���j ��i
r t ENVIRONMENTAL LABORATORIES
a A Division of Water Technology and Controls Inc
Chemical and Physical Determinations
Client: Cla on NPDES#: 0025453 StartDate: 9/27/16
Test Organism: Pimephales prome/as End Date: 10/4/16
Da
Concentration: 0.5%
0-1
Da
2-3
3-4
4-5
5-6J
Concentration:
control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Freshwate
Batch #
0
D.O.: Initial
/
7-
`l `t 1
�6
pH:
Initial
, Z
�9
• "
Final
of !,l-
l p
U -
i4.
9Q .S
- r
vC- .
D.O.
Initial
Final
-7
Ro—e
0-0 wa-
- 0'7
0
(�
Residual Chlorine:
Temp.:
Initial
Final
�`j-�
vQtw
Co . �,
fID14•'1]
15.✓
` Ll •
/,/
Conductivity:
Initial
%
�(`�
�'(�
2-3
-7•7
11)-,,
) J
Ma
it,
6-7
Final
Residual Chlorine:
�i0,)
/-0.
.
as r
7L
(
V
Hardness:
L
7
t1q7
-�
1.01
,q(117
(..(JL(
Alkalinity:
jt!; -43
��
gLi
- '�
_
�U Lf
D t-,, Q
�I.3aL
5LL4 LO
K
Da
Concentration: 0.5%
0-1
1-2
2-3
3-4
4-5
5-6J
6-7
Remarks
pH: Initial
.0
g -
1
agq•
c?.
Final
D.O.: Initial
7-
`l `t 1
�6
1
Final
�9
Temp. Initial
Final
of !,l-
l p
U -
i4.
9Q .S
- r
vC- .
0 -
Conductivity: Initial
Final
as
2310
Ro—e
0-0 wa-
Residual Chlorine:
Co . �,
I' 1
Gel
Day
0-1
Q
1-2
2-3
-7•7
3-4
-0
' el
4-5
- -Z
5-6
-10-
6-7
Remarks
Concentration: 1%
pH: Initial
Final
D.O.: Initial
.
7L
Final
,
-�
1.01
,q(117
(..(JL(
Temp.: Initial
Final
jt!; -43
��
gLi
- '�
_
�U Lf
D t-,, Q
�I.3aL
5LL4 LO
K
Conductivity: Initial
Final
oL
d—
1>1}
% _
Residual Chlorine:
Z 0- k
14. 1
ZdA
I LTJ,

Client: Cla on
/WA=—"07-A=—C:7", ff f4=7
ENVIRONMENTAL L�160 RATO RIES
-a olvi !on ci w err 'nrcnr.o/o9Y ..^v co.,rro/s. /..�.
Chemical and Physical Determinations
NPDES#: 0025453
Test Organism: Pimenhales nromelas
Dav
StartDate: 9/27/16
End Date: 10/4/16
Page 2 of 2
Concentration:
2%
0-1
1-2
-3
3-4
4-5
5-6
6-7 Remarks
pH:
Initial!
)
I !
V
j
�+'-2
. l�
S - 12
'Io `7
Final
1-7,
-
-7,
-O')
D.O.
q7-
"
D.O.:
Initial
—7,&Z�'
�
- i-0
-(
Final
6.5
I
�- l
Final/d-1
,
S
'
Initial
3
il
S
Temp.:
Initial
a
Lt..
154. 1
aq s 1
u- i
Final
Zq6
`a-� (, 2
"IS . 5
fIL4, 1
o7�
91
Conductivity:
Initial
'a
a 14
p�,
9�!�5
' 0
().-K C,
Residual Chlorine:
Final
-CO. l
e-0.1
1
�p
4L, I
11
Residual Chlorine:
Gp,
Gd 1
G0.- i
G vl
• 1
Dav
Concentration:
4%
0-1
1-2
2-3
3-4
4-5
5-6
6-7 Remarks
pH:
Initial
)
I !
� -?-'>L
•
1e�
- 16
S - 12
'Io `7
Final
7
-
?,
-O')
D.O.
InitialP;;;;�
'l.
D.O..
Initial
—7,&Z�'
�
- i-0
-(
Final
6.5
I
�- l
Final
,
S
'
Initial
3
il
_,6.01
Temp.:
Initial
a
2 -
LL 5/
Gu -
2�(-
u- i
Finalk
_ &>
L% LA
rA3
� Lf
95. (L�
)- 6
14,
-
Conductivity:
Conductivity:
Initial
'a
3
p�,
9�!�5
3L•
().-K C,
Residual Chlorine:
Final??
-CO. l
e-0.1
1
W • l
4L, I
Residual Chlorine:
L p, 1
/-o. 1
Z0,
Dav
Concentration:
8%
0-1 1-2
2-3
34
4-5
5-6
6-7 Remarks
pH:
Initial
- 0
)
I !
� -?-'>L
•
1e�
Final
'Io `7
-
-7 '
`f
D.O.
InitialP;;;;�
7 -7_
7 'Z •
Alkalinity:
�
- i-0
Final
6.5
�- l
S
Temp.:
Initial
tl �
o�
�4-�
-
a
Final
_ �`1,�
-2
2�(-
u- i
Conductivity:
Initial
"'off
"L6
a
Final
2,5
1(2
�J
3L•
().-K C,
Residual Chlorine:
Z-0. /-0 ,1
-CO. l
e-0.1
1
W • l
4L, I
Day
Concentration: 100%
0-1
1-2
2-3
3-4
4-5 5-6 6-7
Remarks
PM
Conductivity:
Residual Chlorine:
fid_
CU
Gu+ I
Hardness:
11{
Alkalinity:

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Clayton NPDES#: 0025453 Start Date: 9/27/16
Test Organism: Pimephales promelas End Date: 10/4/16
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
Sunday
Monday
Tuesday Wednesday Thursday
Rep A 0 _
Rep B 0
Rep C 0
Rep D 0
Friday
r"
Rep B 0 0
Friday
0
10
Saturday
0—
0—
O
y —
�—
Tuesday
Rep C 0
©
1(9—
(9
Rep D 0 0
Rep
v —
Concentration 0.5%
Sunday
Monday
Tuesday Wednesday Thursday
Rep A 0 _
Rep B 0
Rep C 0
Rep D 0
Friday
r"
Tuesday Wednesday Thursday
Rep A 0
Rep B 0
Rep C 0 (J D
Friday
0
10
Saturday
0—
0—
O
Sunday
Monday
Tuesday
Rep D 0
Concentration 'I %
Sunday
Monday
Tuesday Wednesday Thursday
Rep A 0 _
Rep B 0
Rep C 0
Rep D 0
Friday
r"
turday
Sunday
Monday
Tuesday
.
O
C/

' MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Claylon NPDES#: 0025453 Start Date: 9127/16
Test Organism: Amenhales promelas End Date- 10/4/16
Concentration 2%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
4%
---
Rep B 0_
`
--�--
Wednesday Thursday
Rep C 0
C)
Sunday
Monday
t
Rep D 0
V
---
V
Concentration
4%
Tuesday
Wednesday Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0_
---
Rep B
0
Rep C
0_
Rep D
0
—--
O
Concentration
8%
Tuesday
Wednesday Thursday
Friday
Saturday
Sunday
Monday
Tuesday
A
0
�
�
�
Rep
Rep B
0
0
C)
Rep C
0
0
y
Q
0
—�
Rep D
0
.(�

E ® TEC 1a,v MIC.
.
ENVIRONMENTAL LABORATORIES
'r ADivision of Waver Technology and Conrrols, lnc.
FISH LARVAL SURVIVAUGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Clayton
NPDES #: 0025453
Date of Test: 9/27/16
Initial weights taken on: by:
Final weights taken on:_LO—by:
Page: 1 of 2
Outfall:
Organism: Pimephales promelas
PAN WT.
(Mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV
CONC
REP
-6
v
A
�3�
43 6 -q
L o-
e 6 S7
• a 7
-70q
1�03
-76
-. 3
/0
10
(0
6-701-
o- �6 3
-���
-
loo
B
c
D
��3� -�
A
-7. t0
3
q .q1
B
�' -•��
[ 7O
D9.0P
g -77
0
o_���
0---77
�v
100
Boa
7
D
-��. �s
1%
A
538,
5
-7.60
10
B
C
��_�
a.
�8 a�
q�?,16
��S a
7 o
10
70a
oda
Z
0
8a Sa37,x1
r1,71-/
10
0--7-71111(90

m ERI ftl C r Ci
ENVIRONMENTAL LABORATORIES
' • A Divisson of water Technology ana controls. Inc
FISH LARVAL SURVIVALIGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Clayton
NPDES #: 0025453
Date of Test: 9/27/16
Initial weights taken on: V - l ( by: L tai
Final weights taken on: i2:� 16 by:
Page: 2 of 2
Outfall:_
Organism: PiM phales pr=elas
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV
CONC
REP
PAN WT.
(mg)
A
r r
`P -I LY -3
`dam
lv
r�,gaa
j
2%
6,D- 30
0-6
0()
(0
0.637
lot)
A
3;H. o
X33 �a
q• /3
B
C_
. W5
o
4%
I gLff.
.�-d\
� 7..
-7-05
1D—
L
0'A
loo
d
/6)
D
F`7
A
6)-
100
8%
, "o'
q.11
D
?3
31
1�
�� �

MERITECH, INC. Mentech Sample ID 0. G
+ Bioassay Sampfe Chaln of Custody
;A642 Tamco Rd. Reidsville NC 27320
N
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: [e-mail] meritech-labs.com Web Site. www.meritech-labs.com
CLIENT INFORMATION
Client
�n[J�+1 �1C Yro��
Af
PO#:
Time:
'r'
,,��/]]
`i7'
NPDES#. NC QO;Z 5:- �
Contact Person.
nLs l.J�:Y —�
AM
Phone
Address:
��
AM
PM
City:
L14, 2/{J -'J
AM
Pipe #-.
County.
Za"9 ..J-SState: ��
Zip:
PM
Time:
SAMPLE INFORMATION
AM
PM
SampleSite:
L -.!7r) t'i C re - e -k- �1'J7-
AM
PM
Sample Type:
❑ Grab [Composite # of containers:
Sampling Time:
Start Date: -L� MO Start Time: /y/a
AM
PM
/9
End Date: 7 -Z % - /�e End Time: 9: 10
AM
PM
**` Triple rinse sample container with sample before filling. Completely till the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory***
Collector's Name: Print L.�t �11Ct•"1 d2 Sr'/�S u�•,� Signature:
TOXICITY TEST INFORMATION
Test Required: >.Ghronic (7 days)
❑ Acute (24-48 hours)
Test Organism: OL Ceriodaphnia dubia (water flea)
[.Pimephales promelas (fathead minnow)
p Mysidopsis bahfa (shrimp)
Test Concentrations (if multiple dilutions)*
Comments:
SHIPPING INFORMATION
Relinquished by:
Received by -
Relinquished by;,
Received by`
Relinquished by:
Received by:
Relinquished by:
Received by:
Date:
Date:
Date:
Date:
Date:
Date:
Date:
Date:
Sample Temperature ("C):
Time:
C "
Af
PM
Time:
t!/
,,��/]]
`i7'
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Method of Shipment:❑ UPS ❑ Fed EX ;?�,Meritech Pick-up Delivered ❑ Other
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED **
SAMPLE RECEIVING (Laboratory Use Only)
—f'jtj
Relinquished by: W"�
Date: y Time. - y AM PM
Received by- U
Sample Temperatures CC):C+ i I � � �1 / Sample Condition:
WHITE = Laboratory coov YELLOW = Client cony

MERITECH, INC.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Mentech Sample ID It
eritech-labs.com
Toxicity Supervisor email: [e-mail] meritech4abs.com Web Site. www.m
CLIENT INFORMATION
Client:
NPDES#' NC 00A6'13 73
Contact Person
P�©%� �7 Phone: J�'/ ✓S�%S 3��
Address:
Pipe #:
City:
County: riL�.T/SX��t/ State: Zip: �.~ls.Z.S�
SAMPLE INFORMATION
Sample Site: •e � �
# of containers: ati
Sample Type: ❑ Grab Composite
%D_i 3 d /'1
9 -Aa % Start Time:�� ( I� PM
Sampling Time: Start Date:
End Date:4-P-9 -/4 End Timer AM PM
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory***
e c— t S �k,/�SD��/ Signature:
Collector's Name: Pring %jt
TOXICITY TEST INFORMATION
Test Required: -Chronic (7 days)
❑ Acute (24-48 hours)
IWC: t %
Comments:
`perp SHIPPING INFORMATION
Relinquished by: .mo i : Date:
Received by ` Date.
Relinquished by. CfI Date:
Date:
Received by:
Test Organism: D�4:Ceriodaphnia dubia (water flea)
T<-Pimepha/es promelas (fathead minnow)
Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions):
Relinquished by:
Received by:
Relinquished by:
Received by.
Method of Shipment ❑ UPS
Date:
Date:
Date:
Date:
Sample Temperature CC):
Time:
Time:
Time: 1-6
Time -
Time:
Time:
Time:
Time:
❑ Fed EX ❑ Meritech Pick-up E] Delivered ❑ Other
AM PM
PM
AM PO
AM PM
AM PM
AM PM
AM PM
AM PM
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED
SAMPLE RECENING (Laboratory Use Only)
Relinquished by: a . rj
Date: 5 G Time: t S l AM
Received by:
Sample Temperatures CC): �_1 / 1 Sample Condition:
WNITF = 1 ahnratnev rnnv yFl 1 nw = i rant rnnv

MERITECH, INC. Mentech Sample ID A.
Bloassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
A<0 Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike.reedCa)meritech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client f Q �7"[�� PO#:
Contact Person:. 1l:Lyr/��6 5 L ��yghr/�-�J NPDES#• NCA0 z5"4,5
Address: Q Ox t� ! Phone: 219
City Pipe #•
County:
State: XAr Zip:%J a2 511
J SAMPLE INFORMATIONj�
Sample Site: L. i jYY-0— / E= � /C- u //�(j ��J�i�--
J -
Sample Type: ❑ Grab 5Lomposite # of containers:
Sampling Time: Start Date. 7-,7-1-/6 StartTime. AM PM
End Date: -'30'/6, End Time: PM
*`* Triple rinse sample container with sample before filling. Completely till the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.LrC upon receipt at the laboratory'*"
Collector's Name: PrintSignature:✓- -- '
Test Required: Chronic (7 days)
❑ Acute (24-48 hours)
IWC: )., e) %
Comments:
TOXICITY TEST INFORMATION
Test Organism: MiCer/odaphnia dubia (water flea)
;�fPimephales prone/as (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions) -
SHIPPING INFORMATION
Relinquished by ��i��.=�..,1� g�-� -
Date: �- 3B ��
Time:
167,' AM PM
Received by.
Date: 13 d ll 6j
Time:
Z SS" AM PM
�����- —�
l
Date: �3 O �J(f p
Time:
uO
� I AM PM
Relinquished by:
Received by:
Date:
Time:
AM PM
Relinquished by:
Date:
Time:
AM PM
Received by:
Date:
Time:
AM PM
Relinquished by:
Date:
Time:
AM PM
Received by:
Date:
Time:
AM PM
Sample Temperature CC):
Method of Shipment ❑ UPS ❑ Fed EX
❑ Meritech Pick-up ❑ Delivered
❑ Other
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
Received by: ` Date: U l Time: 1 U AM
Sample Temperatures CC): _LL / / / Sample Condition: Q ` f
WHITE = Laboratory copy YELLOW = Client coov

L
Title: Clayton NO TRANSFORMATION
File: ClayGrowth Transform:
Shapiro - Wilk's Test for Normality
-------------- -------------------------------------------------------------
D = 573130.9713
W = 0.4656
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning - The first three homogeneity tests are sensitive to non -normality
and should not be performed with this data as is.

Title: ClaytonTransform: NO TRANSFORMATION
File: ClayGrowth
Bartlett's Test for Homogeneity of Variance ---------
---------------------------
Calculated B1 statistic = 195.3776
(p -value = 0.0000)
Data FAIL Bi homogeneity test at 0.01 level. Try another transformation.
----------------------- ----------------------------------------------------
Critical B _ 11.O705 (alpha = 0.05, df = 5)

I O1 Ul to W N
rt
' I b
pi
I
I , 1
pi
N
, I
H ; �rrr
0
I 1
(D
�N
m' I
4
I
I
:D
µ ' o cit i Z
p I n i
'i :jF'- I OD IP N f1 Ul O I I (D
(D I I ,d
a O ,
I N I H
I 75'
X ' p I 0
I �000000 i Z i CD
L9 I vJ NrAiArP I
I N 0) of -4 Ln CO I H K
I O co O O W 0 i z I
I
3 I U1
I I O
1 I I
I00000 I Ix
I O o o 0 o I 1 0
1 , ..
1 ,
1 0 0 0 0 0
I
o o 0 0 0 I i F --j O
I
H H
I
CD
I I �
I IP IP FP �P rP � � I iv
I OOOoO
I
I O O O O O I(D 0
C) I 0I , CJ] t t•1'
I Ln C,1 ' H
O
I z

Title: Clayton Transform: NO TRANSFORMATION
File: ClaySury
Shapiro - Wilk's Test for Normality ------------------
------ ---------------- -----------------
----------------------------
D = 0.0150
W = 0.6138
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
----------------------------------------------------------------------------
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning - The first three homogeneity tests are sensitive to non -normality
and should not be performed with this data as is.

Title: Clayton Transform: NO TRANSFORMATION
File: C1aySury
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance _
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.

D
Title: Clayton
File: C1aySury
Transform:
NO TRANSFORMATION
Steel's Many -One
Rank Test
- Ho:
Control<Treatment
----------------------------------------------------------------------------
MEAN IN
RANK
CRIT.
SIG
GROUP
IDENTIFICATION
ORIGINAL UNITS
-----------
SUM
-------
VALUE
------
DF 0.05
----- ---
-------------------------
1
Control
1.0000
2
0.5%
0.9750
16.00
10.00
4.00
3
1%
1.0000
18.00
10.00
4.00
4
2%
0.9750
16.00
10.00
4.00
5
40-.
1.0000
18.00
10.00
4.00
6
8%
1.0000
18.00
10.00
4.00
----------------------------------------------------------------------------
Critical
values are 1 tailed ( k = 5 )