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NC0020389_Renewal Application_20170818
Water Resources ENVIRONMENTAL QUALITY September 18, 2017 Matthew Zapp, Manager Town Town of Benson PO Box 69 Benson, NC 27504-0069 Subject: Permit Renewal Application No. NCO020389 Benson WWTP Johnston County Dear Applicant: ROY COOPER Governor MICHAEL S. REGAN sectetary S. JAY ZIMMERMAN Director The Water Quality Permitting Section acknowledges the September 18, 2017 receipt of your permit renewal application and supporting documentation. Your application will be assigned to a permit writer within the Section's NPDES WW permitting branch. Per G.S. 150B-3 your current permit does not expire until permit decision on the application is made. Continuation of the current permit is contingent on timely and sufficient application for renewal of the current permit. The permit writer will contact you if additional information is required to complete your permit renewal. Please respond in a timely manner to requests for additional information necessary to allow a complete review of the application and renewal of the permit. Information regarding the status of your renewal application can be found online using the Department of Environmental Quality's Environmental Application Tracker at: https://deg.nc.ciov/permits-regulations/permit guidance/environmental-application-tracker If you have any additional questions about the permit, please contact the primary reviewer of the application using the links available within the Application Tracker. Sincerrely, ,3, Wren Thedford Administrative Assistant Water Quality Permitting Section cc: Central Files w/application ec: WQPS Laserfiche File w/application State of North Carolina I Environmental Quality I Water Resources 1617 Mail Service Center I Raleigh, North Carolina 27699-1617 919-807-6300 THE WOOTEN COMPANY 301 West 14`r' Street Greenville, NC 27834 252 757 1096 Fax 252 757 3221 September 15, 2017 Mr. John Hennessy Supervisor — Compliance & Expedited Permitting Unit Division of Water Quality Surface Water Protection Section - NPDES 512 N. Salisbury Street 9th Floor — Archdale Bldg. Raleigh, NC 27604 Re: NPDES Permit Renewal (NC0020389) Town of Benson, NC Dear Mr. Hennessy: RECEIVEMCDEQIDWR SEP 18 2017 Water Quality Permitting Section Please find attached three (3) copies of the information necessary for the Town of Benson to renew NPDES Permit # NC0020389. The following attachments include the NPDES Forms 1 & 2A, a topographic map of the area, a process flow diagram, a sludge management plan, as well as the results from priority pollutant analyses and toxicity testing. If you have any questions or should need any additional information please feel free to contact our office at (252)-757-1096. Best regards, The Wooten Company .Z,By: William A. Lar en, P.E. cc. Matt Zapp, Benson Town Manager TWC File RECEIVEMCDEUDWR SEP 18 2017 Water Q Section Permitting ruEaf"" RENEWAL APPLICATION BENSON WASTEWATER TREATMENT PLANT TOWN OF BENSON, NORTH CAROLINA SEPTEMBER 2017 j PREPARED BY: THE WOOTE N COMPANY 301 WEST 14TH STREET GREENVILLE, NC 27834 LICENSE No.: F-0115 RECEIV EDINCDEQIDWR SEP 18 2017 1Rtatet ` Sect on Permitting Application for Renewal of NPDES Permit No. NCO0203 89 Town of Benson, North Carolina Prepared by: The Wooten Company 301 W. 14th Street Greenville, North Carolina 27834 (252)-757-1096 EPA Form 1 Please print or type in the unshaded areas only Form Approved OMB No 2040-0086 FORM US ENVIRONMENTAL PROTECTION AGENCY I EPA I D NUMBER 1 NEPA GENERAL INFORMATION 6 PA C Consolidated Permits Program F NC 0 0 2 0 3 8 9 D GENERAL (Read the "General Instructions " before starting) , 2 11 14 13 LABEL ITEMS NCO020389 Town of Benson VVVVTP GENERAL If a preprinted label has INSTRUCTIONS been provided, affix it in the designated space Review the information carefully, if any of it is incorrect, cross through it and enter the correct data in the I EPA I D NUMBER P.O. Box 6 uPLEASE PLACE LABEL IN THIS SPACE appropriate fill-in area below Also, if any of the prepnnted data is absent (the area to the left of the label space lists the III FACILITY NAME 303 East Church Street information that should appear), please provide it in the proper fill-in area(s) below If the label is complete and correct, you need Items I, III, V, VI VI V FACILITY MAILING ADDRESS not complete and (except -B which must be completed regardless) Complete all items if no label Benson, NC 27504 has been provided Refer to the instructions for detailed item VI FACILITY LOCATION descriptions and for the legal authorizations under which this data is collected II POLLUTANT CHARACTERISTICS INSTRUCTIONS Complete A through J to determine whether you need to submit any permit application forms to the EPA If you answer "yes" to any questions, you must submit this form and the supplemental form listed in the parenthesis following the question Mark "X" in the box in the third column if the supplemental form is attached If you answer "no" to each question, you need not submit any of these forms You may answer "no" if your activity is excluded from permit requirements, see Section C of the instructions See also, Section D of the instructions for definitions of bold-faced terms Mark *X" Mark W ves NO FARM ATTACHED ves No FORM ATTACHED SPECIFIC QUESTIONS SPECIFIC QUESTIONS A Is this facility a publicly owned treatment works which results in a discharge to waters of the U S ? (FORM 2A) X X B Does or will this facility (either existing or proposed) include a concentrated animal feeding operation or X aquatic animal production facility which results in a 16 17 16 19 m z, discharge to waters of the U S ? (FORM 2B) C Is this a facility which currently results in discharges to X D Is this a proposed facility (other than those described to A waters of the U S. other than those described in A or B or B above) which will result in a discharge to waters of X above') (FORM 2C) the U.S ? (FORM 2D) ss 1 ze 17 22 M s4 E Does or will this facility treat, store, or dispose of F Do you or will you inject at this facility industrial or hazardous wastes9 (FORM 3) X municipal effluent below the lowermost stratum X containing, within one quarter mile of the well bore, underground sources of drinking water? (FORM 4) 31 32 33 ze G Do you or will you inject at this facility any produced water H Do you or will you inject at this facility fluids for special or other fluids which are brought to the surface in connection with conventional oil or natural gas production, X processes such as mining of sulfur by the Frasch process, solution mining of minerals, in situ combustion of fossil X inject fluids used for enhanced recovery of oil or natural fuel, or recovery of geothermal energy? (FORM 4) gas, or inject fluids for storage of liquid hydrocarbons (FORM 4) W 36 39 x 35 36 I Is this facility a proposed stationary source which is one J Is this facility a proposed stationary source which is of the 28 industrial categones listed in the instructions and�/ X NOT one of the 28 industrial categories listed in the X which will potentially emit 100 tons per year of any air instructions and which will potentially emit 250 tons per pollutant regulated under the Clean Air Act and may affect I I year of any air pollutant regulated under the Clean Air Act w 41 42 43 45 or be located in an attainment area? (FORM 5) and may affect or be located in an attainment area? (FORM 5) III NAME OF FACILITY SKIP I Benson Wastewater Treatment Plant J1C 30 69 IV FACILITY CONTACT A NAME & TITLE (last, first, & title) B PHONE (area code & no) c 2 Matthew Zapp, own Manager ( 1) 8 4-3 5 ,5 16 45 46 46 49 41 SZ- 55 V FACILTY MAILING ADDRESS A STREET OR P O BOX c 3 Post Office Box 69 ,5 ,6 d5 B CITY OR TOWN C STATE D ZIP CODE NaiBenson C 2 5 4A 40 41 42 47 51 VI FACILITY LOCATION A STREET, ROUTE NO OR OTHER SPECIFIC IDENTIFIER 15c 7 0 Hanna Cree 'Road 5 is 05 B COUNTY NAME Jc Ston 46 ]0 C CITY OR TOWN D STATE I E ZIP CODE F COUNTY CODE (ifknown) c 6 F u a s C 2 5 4 15 16 dD 41 4Z 47 51 52 -54 EPA Form 3510-1 (8-90) CONTINUE ON REVERSE r.ONTINI IFr1 PROM THF FRr1NT VII SIC CODES (4 -digit, in orderof non A FIRST B SECOND ° (speck) 7 (specify) 7 15 18 19 15 t6 19 C THIRD D FOURTH C (spec) (specify) 7 15 18 19 15 16 19 VIII OPERATOR INFORMATION A NAME B Is the name listed in Item Town of Benson VIII -A also the owner B 0 YES ❑ NO ,g 16 55 66 C STATUS OF OPERATOR (Enter the appropriate letter into the answer box if "Other, "specify) ID PHONE (area code & no) F = FEDERAL S = STATE M = PUBLIC than federal or stare) M (spec) ` N/A A ( 919) 894-3553 specs O =OTHER (specify) P = PRIVATE 15 56 6 - 18 Ila 21 22 26 E STREET OR P O BOX FT Post Office ox 69 26 55 F CITY OR TOWN G STATE I H ZIP CODE JIX INDIAN LAND °Is the facility located on Indian lands) B Benson NC 1127-504 ❑ YES 13 NO 52 15 18 d0 41 d2 47 - 51 X EXISTING ENVIRONMENTAL PERMITS A NPDES Drschar ,st ace Water D PSD (Air Emrssrons roiPro osedS6unT C r c r i 9 N NCO020389 15 16 ,7 ,6 30 15 16 17 18 30 B UICUnder r ectrono Fluids E OTHER s ecr 9 U qdm Biosoli s Land A icion 9 pp a (spec) WQ0012514 15 16 17 18 30 15 18 ,] 18 38 C RCRA Hazardous Wastes E OTHER s ecr c r c r (specify)9 R 9 ,5 18 17 18 30 1 15 1 18 1 17 118 30 XI MAP Attach to this application a topographic map of the area extending to at least one mile beyond property boundaries The map must show the outline of the facility, the location of each of Its existing and proposed intake and discharge structures, each of its hazardous waste treatment, storage, or disposal facilities, and each well where It Injects fluids underground Include all springs, rivers, and other surface water bodies in the map area See Instructions for precise requirements XII NATURE OF BUSINESS (provide a bnef descnption) Treatment and Disposal of Municipal wastewater XIII CERTIFICATION (see instructions) I certify under penalty of law that I have personally examined and am familiar with the information submitted in this application and all attachments and that, based on my inquiry of those persons immediately responsible for obtaining the information contained in the application, I believe that the information is true, accurate, and complete I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment A NAME & OFFICIAL TITLE (type orpnnt) B SIGNATURE C DATE SIGNED Matthew Zapp, Town Manager COMMENTS FOR OFFICIAL USE ONLY 1,i is EPA Form 3510-1 (8-90) EPA Form 2A (includes Parts A, B, C, D, E, and F) FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED RIVER BASIN Benson WWTP NCO020389 Renewa Neuse FORM 2A NPDES FORM 2A APPLICATION OVERVIEW NPDES APPLICATION OVERVIEW Form 2A has been developed in a modular format and consists of a "Basic Application Information" packet and a "Supplemental Application Information" packet. The Basic Application Information packet is divided into two parts. All applicants must complete Parts A and C. Applicants with a design flow greater than or equal to 0.1 mgd must also complete Part B. Some applicants must also complete the Supplemental Application Information packet. The following items explain which parts of Form 2A you must complete. BASIC APPLICATION INFORMATION: A. Basic Application Information for all Applicants All applicants must complete questions A 1 through A 8 A treatment works that discharges effluent to surface waters of the United States must also answer questions A 9 through A 12 B. Additional Application Information for Applicants with a Design Flow z 0.1 mgd. All treatment works that have design flows greater than or equal to 0 1 million gallons per day must complete questions B 1 through B 6 C. Certification All applicants must complete Part C (Certification) SUPPLEMENTAL APPLICATION INFORMATION: D. Expanded Effluent Testing Data. A treatment works that discharges effluent to surface waters of the United States and meets one or more of the following criteria must complete Part D (Expanded Effluent Testing Data) 1 Has a design flow rate greater than or equal to 1 mgd, 2 Is required to have a pretreatment program (or has one in place), or 3 Is otherwise required by the permitting authority to provide the information E. Toxicity Testing Data. A treatment works that meets one or more of the following criteria must complete Part E (Toxicity Testing Data) 1 Has a design flow rate greater than or equal to 1 mgd, 2 Is required to have a pretreatment program (or has one in place), or 3 Is otherwise required by the permitting authority to submit results of toxicity testing F. Industrial User Discharges and RCRA/CERCLA Wastes. A treatment works that accepts process wastewater from any significant industrial users (SIUs) or receives RCRA or CERCLA wastes must complete Part F (Industrial User Discharges and RCRA/CERCLA Wastes) SIUs are defined as 1 All industrial users subject to Categorical Pretreatment Standards under 40 Code of Federal Regulations (CFR) 403 6 and 40 CFR Chapter I, Subchapter N (see instructions), and 2 Any other industrial user that a Discharges an average of 25,000 gallons per day or more of process wastewater to the treatment works (with certain exclusions), or b Contributes a process wastestream that makes up 5 percent or more of the average dry weather hydraulic or organic capacity of the treatment plant, or c Is designated as an SIU by the control authority G. Combined Sewer Systems. A treatment works that has a combined sewer system must complete Part G (Combined Sewer Systems) ALL APPLICANTS MUST COMPLETE PART C (CERTIFICATION) EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 1 of 22 FACILITY NAME AND PERMIT NUMBER- PERMIT ACTION REQUESTED RIVER BASIN Benson WWTP NCO020389 Renewa Neuse BASIC APPLICATION INFORMATION PART A. BASIC APPLICATION INFORMATION FOR ALL APPLICANTS: All treatment works must complete questions A 1 through A.8 of this Basic Application Information Packet A.1 Facility Information. Facility Name Benson Wastewater Treatment Plant Mailing Address PO Box 69 303 East Church Street Benson, NC 27504 Contact Person Mathew Zapp Title Town Manager Telephone Number (919) 894-3553 Facility Address 770 Hannah Creek Road (not P O Box) Four Oaks, NC 27524 A 2 Applicant Information. If the applicant is different from the above, provide the following APPLICATION PREPARER Applicant Name Town of Benson The Wooten Company Mailing Address PO Box 69 303 East Church Stree 301 West 14th Street Benson, NC 27504 Greenville, NC 27834 Contact Person Mathew Zapp William A Larsen, P E Title Town Manager Proiect Engineer Telephone Number (919) 894-3553 (252) 757-1096 Is the applicant the owner or operator (or both) of the treatment works? ® owner ❑ operator Indicate whether correspondence regarding this permit should be directed to the facility or the applicant ❑ facility ® applicant A.3 Existing Environmental Permits Provide the permit number of any existing environmental permits that have been issued to the treatment works (include state -issued permits) NPDES NCO020389 PSD UIC Other WQ0012514 RCRA Other WQ0022501 A 4 Collection System Information Provide information on municipalities and areas served by the facility Provide the name and population of each entity and, if known, provide information on the type of collection system (combined vs separate) and its ownership (municipal, private, etc ) Name Population Served Type of Collection System Ownership Benson 3,320 Separate Municipal Total population served 3,320 EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 2 of 22 FACILITY NAME AND PERMIT NUMBER. PERMIT ACTION REQUESTED: RIVER BASIN: Benson WWTP, NCO020389 I Renewal Neuse A.5. Indian Country a Is the treatment works located in Indian Country? ❑ Yes N No b Does the treatment works discharge to a receiving water that is either in Indian Country or that is upstream from (and eventually flows through) Indian Country? ❑ Yes N No A.6. Flow. Indicate the design flow rate of the treatment plant (i a, the wastewater flow rate that the plant was built to handle) Also provide the average daily flow rate and maximum daily flow rate for each of the last three years Each year's data must be based on a 12 -month time period with the 12�" month of "this year" occurring no more than three months prior to this application submittal a Design flow rate 1.9 mgd Two Years Ago Last Year This Year b Annual average daily flow rate 0 990 1.048 0.967 c Maximum daily flow rate 3.867 4.407 5 035 A.7. Collection System. Indicate the type(s) of collection system(s) used by the treatment plant Check all that apply Also estimate the percent contribution (by miles) of each N Separate sanitary sewer 100 % ❑ Combined storm and sanitary sewer % A 8. Discharges and Other Disposal Methods. a Does the treatment works discharge effluent to waters of the U S 7 ® Yes ❑ No If yes, list how many of each of the following types of discharge points the treatment works uses I Discharges of treated effluent n Discharges of untreated or partially treated effluent 0 ni Combined sewer overflow points 0 IV Constructed emergency overflows (prior to the headworks) 0 V Other n/a 0 b Does the treatment works discharge effluent to basins, ponds, or other surface impoundments that do not have outlets for discharge to waters of the U S ? ❑ Yes N No If yes, provide the following for each surface impoundment Location n/a Annual average daily volume discharge to surface impoundment(s) Is discharge ❑ continuous or ❑ intermittent? c Does the treatment works land -apply treated wastewater? If yes, provide the following for each land application site Location n/a Number of acres n/a n/a mgd ❑ Yes N No Annual average daily volume applied to site n/a mgd Is land application ❑ continuous or ❑ intermittent? d Does the treatment works discharge or transport treated or untreated wastewater to another treatment works? ❑ Yes N No IEPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 3 of 22 FACILITY NAME AND PERMIT NUMBER. PERMIT ACTION REQUESTED: RIVER BASIN: Benson WWTP, NCO020389 Renewal Neuse If yes, describe the mean(s) by which the wastewater from the treatment works is discharged or transported to the other treatment works (e g , tank truck, pipe) n/a If transport is by a party other than the applicant, provide Transporter Name n/a Mailing Address n/a n/a Contact Person n/a Title n/a Telephone Number 1 n/a For each treatment works that receives this discharge, provide the following Name n/a Mailing Address n/a n/a Contact Person n/a Title n/a Telephone Number ) n/a If known, provide the NPDES permit number of the treatment works that receives this discharge n/a Provide the average daily flow rate from the treatment works into the receiving facility n/a mgd e Does the treatment works discharge or dispose of its wastewater in a manner not included in A 8 through A 8 d above (e g, underground percolation, well injection) ❑ Yes ® No If yes, provide the following for each disposal method Description of method (including location and size of site(s) if applicable) n/a Annual daily volume disposed by this method n/a Is disposal through this method ❑ continuous or ❑ intermittent? EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 4 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: Benson WWTP, NCO020389 Renewal Neuse WASTEWATER DISCHARGES: If you answered "Yes" to question A 8 a, complete questions A.9 through A 12 once for each outfall (including bypass points) through which effluent is discharged. Do not include information on combined sewer overflows in this section. If you answered "No" to question A.8.a, go to Part B, "Additional Application Information for Applicants with a Design Flow Greater than or Equal to 0.1 mgd " A.9 Description of Outfall. a Outfall number b Location Four Oaks 27524 (City or town, if applicable) (Zip Code) Johnston NC (County) (State) 35" 23' 12" N 78° 30'29"W (Latitude) (Longitude) c Distance from shore (If applicable) n/a ft d Depth below surface (if applicable) n/a ft e Average dally flow rate 0 967 mgd f Does this outfall have either an intermittent or a periodic discharge? ❑ Yes ® No (go to A 9 g ) If yes, provide the following Information Number f times per year discharge occurs Average duration of each discharge Average flow per discharge n/a mgd Months in which discharge occurs n/a g Is outfall equipped with a diffuser? ❑ Yes ® No A 10. Description of Receiving Waters. a Name of receiving water Hannah Creek b Name of watershed (If known) Neuse River Basin United States Soil Conservation Service 14 -digit watershed code (if known) c Name of State Management/River Basin (if known) Neuse United States Geological Survey 8 -digit hydrologic cataloging unit code (if known) 030404 d Critical low flow of receiving stream (if applicable) acute cfs e Total hardness of receiving stream at critical low flow (if applicable) chronic cfs mg/I of CaCO3 EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 5 of 22 FACILITY NAME AND PERMIT NUMBER PERMIT ACTION REQUESTED RIVER BASIN Benson WWTP NCO020389 Renewa Neuse All Description of Treatment a What level of treatment are provided? Check all that apply ® Primary ® Secondary ® Advanced ❑ Other Describe b Indicate the following removal rates (as applicable) Design BOD5 removal or Design CBOD5 removal 98 % Design SS removal 85 % Design P removal 67 % Design N removal 90 % Other % c What type of disinfection is used for the effluent from this outfall? If disinfection vanes by season, please describe Gas Ch onnat on and L qu d Dech onnat on If disinfection is by chlorination is dechlonnation used for this outfall? ® Yes ❑ No Does the treatment plant have post aeration? ® Yes ❑ No A 12. Effluent Testing Information All Applicants that discharge to waters of the US must provide effluent testing data for the following parameters Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include information on combined sewer overflows in this section All information reported must be based on data collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136 At a minimum, effluent testing data must be based on at least three samples and must be no more than four and one-half years apart Outfall number 001 MAXIMUM DAILY VALUE AVERAGE DAILY VALUE PARAMETER Value Units Value Units Number of Samples pH (Minimum) 6.02 s u 1124 pH (Maximum) 744 s u Flow Rate 4 407 mgd 1.016 m d Temperature (Winter) 251 C. 15.4 Co 786 Temperature (Summer) 31 0 C° 22.6 C. 786 For pH please report a minimum and a maximum daily value MAXIMUM DAILY AVERAGE DAILY DISCHARGE POLLUTANT DISCHARGE ANALYTICAL ML/MDL Number of METHOD Conc. Units Conc Units Samples CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS BIOCHEMICAL OXYGEN BOD5 160 m /L 29 m /L 786 00310 2.5 DEMAND (Report one) CBOD5 FECAL COLIFORM 200 #/100nnL 5 #/100mL 783 31616 1 TOTAL SUSPENDED SOLIDS (TSS) 201 mg/L 24 mg/L 786 00530 2 5 END OF PART A. REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS OF FORM 2A YOU MUST COMPLETE EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 6 of 22 FACILITY NAME AND PERMIT NUMBER. PERMIT ACTION REQUESTED. RIVER BASIN: Benson WWTP, NCO020389 Renewal Neuse PART, -A06fT1,6HAL-A:kL,VPAnQN 1,NVORMATION FO@ �OP #110) A 69SI -L( Mat-WTHAN OR LP�1-1117 )VV13-RJ� T - - , -- -': - W 146 dd gallons per dbw. 'WOACT6 6A 46-6 0,0 0 All applicants with a design flow rate �t 0 1 mgd must answer questions B.1 through B.6. All others go to Part C (Certification). B I Inflow and Infiltration. Estimate the average number of gallons per day that flow into the treatment works from inflow and/or infiltration —60,000 gpd Briefly explain any steps underway or planned to minimize inflow and infiltration The Town has an ongoing sewer rehabilitation program to identify and correct infiltration and inflow sources The Town completed a major sewer rehabilitation project in 2005 B 2 Topographic Map. Attach to this application a topographic map of the area extending at least one mile beyond facility property boundaries This map must show the outline of the facility and the following information (You may submit more than one map if one map does not show the entire area ) a The area surrounding the treatment plant, including all unit processes b The major pipes or other structures through which wastewater enters the treatment works and the pipes or other structures through which treated wastewater is discharged from the treatment plant Include outfalls from bypass piping, if applicable c Each well where wastewater from the treatment plant is injected underground d Wells, springs, other surface water bodies, and drinking water wells that are 1) within'/ mile of the property boundaries of the treatment works, and 2) listed in public record or otherwise known to the applicant e Any areas where the sewage sludge produced by the treatment works is stored, treated, or disposed f If the treatment works receives waste that is classified as hazardous under the Resource Conservation and Recovery Act (RCRA) by truck, rail, or special pipe, show on the map where the hazardous waste enters the treatment works and where it is treated, stored, and/or disposed B 3. Process Flow Diagram or Schematic Provide a diagram showing the processes of the treatment plant, including all bypass piping and all backup power sources or redunancy in the system Also provide a water balance showing all treatment units, including disinfection (e g , chlorination and dechlorination) The water balance must show daily average flow rates at influent and discharge points and approximate daily flow rates between treatment units Include a brief narrative description of the diagram B.4 Operation/Maintenance Performed by Contractor(s). Are any operational or maintenance aspects (related to wastewater treatment and effluent quality) of the treatment works the responsibility of a contractor? D Yes 0 No If yes, list the name, address, telephone number, and status of each contractor and describe the contractor's responsibilities (attach additional pages if necessary) Name n/a Mailing Address n/a n/a Telephone Number n/a Responsibilities of Contractor n/a B 5 Scheduled improvements and Schedules of Implementation Provide information on any uncompleted implementation schedule or uncompleted plans for improvements that will affect the wastewater treatment, effluent quality, or design capacity of the treatment works if the treatment works has several different implementation schedules or is planning several improvements, submit separate responses to question B 5 for each (If none, go to question B 6 ) a List the outfall number (assigned in question A 9) for each outfall that is covered by this implementation schedule n/a b Indicate whether the planned improvements or implementation schedule are required by local, State, or Federal agencies 0 Yes 0 No EPA Form 3610-2A (Rev 1-99) Replaces EPA forms 7650-6 & 7550-22 Page 7 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED. RIVER BASIN Benson WWTP, NCO020389 Renewal Neuse c If the answer to B 5 b is "Yes," briefly describe, including new maximum daily inflow rate (if applicable) n/a d Provide dates imposed by any compliance schedule or any actual dates of completion for the implementation steps listed below, as applicable For improvements planned independently of local, State, or Federal agencies, indicate planned or actual completion dates, as applicable Indicate dates as accurately as possible Schedule Actual Completion Implementation Stage MM/DD/YYYY MM/DD/YYYY Begin Construction /na/ /na/ End Construction /na/ /na/ - Begin Discharge /na/ /na/ Attain Operational Level /na/ /na/ e Have appropriate permits/clearances concerning other Federal/State requirements been obtained? ❑ Yes ❑ No Describe briefly n/a B 6. EFFLUENT TESTING DATA (GREATER THAN 0 1 MGD ONLY) Applicants that discharge to waters of the US must provide effluent testing data for the following parameters. Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include information on combine sewer overflows in this section. All information reported must be based on data collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a minimum effluent testing data must be based on at least three pollutant scans and must be no more than four and on-half years old. Outfall Number 001 MAXIMUM DAILY AVERAGE DAILY DISCHARGE DISCHARGE ANALYTICAL ML/MDL POLLUTANT METHOD Number of Conc Units Conc. Units Samples CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS AMMONIA (as N) 6.88 mg/L 032 mg/L 763 00610 0.04 CHLORINE (TOTAL 20 pg/L 6 pg/L 785 50060 10 RESIDUAL, TRC) DISSOLVED OXYGEN 26.1 mg/L 8.8 mg/L 786 00300 7.5 TOTAL KJELDAHL 19.30 mg/L 144 mg/L 164 00625 0.1 NITROGEN (TKN) NITRATE PLUS NITRITE 16.86 mg/L 2.94 mg/L 164 00630 004 NITROGEN OIL and GREASE <5.0 mg/L <5.0 mg/L 2 SM5520B 5.0 PHOSPHORUS (Total) 1 96 mg/L 128 mg/L 170 00665 001 TOTAL DISSOLVED SOLIDS 298 mg/L 247 mg/L 2 EPA160 1 20 (TDS) OTHER sJr.€/ill�F'-vQ; _ r .`a 0 M"_R-, A RWT-i ' # ' �F' ' a �_ ► i i� v E �+ � +I °°i��► ��� ��? � may, ����� o eseao� N3��-Jul- �!��V�-.i' .rF��. a,, , • _A �"��.^?i<���? - -.. . ..���!�" _ +:Ja4nx,Y.. - — 1fJ•nlHE:S�es...Jck"Is,1t,. _ ..�.--cE+.ma�,. _ - -Mnn:.yYa�'•—.`'.�„t45-�.1.�wn-..vre t..a,. .Mum... EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 8 of 22 FACILITY NAME AND PERMIT NUMBER PERMIT ACTION REQUESTED RIVER BASIN: Benson WWTP NCO020389 Renewal Neuse BASIC APPLICATION INFORMATION PART C. CERTIFICATION All applicants must complete the Certification Section. Refer to instructions to determine who is an officer for the purposes of this certification. All applicants must complete all applicable sections of Form 2A, as explained in the Application Overview. Indicate below which parts of Form 2A you have completed and are submitting. By signing this certification statement, applicants confirm that they have reviewed Form 2A and have completed all sections that apply to the facility for which this application is submitted. Indicate which parts of Form 2A you have completed and are submitting: ® Basic Application Information packet Supplemental Application Information packet ® Part D (Expanded Effluent Testing Data) ® Part E (Toxicity Testing Biomonitonng Data) ® Part F (Industrial User Discharges and RCRA/CERCLA Wastes) ❑ Part G (Combined Sewer Systems) ALL APPLICANTS MUST COMPLETE THE FOLLOWING CERTIFICATION. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system or those persons directly responsible for gathering the information, the information is, to the best of my knowledge and belief, true, accurate, and complete I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations Name and official title Matthew Zapp Town Manager SignatureAJI/� Telephone number (919) 8943553 Date signed e) Z/ Upon request of the permitting authority, you must submit any other information necessary to assure wastewater treatment practices at the treatment works or identify appropriate permitting requirements SEND COMPLETED FORMS TO: NCDENR/ DWQ Attn: NPDES Unit 1617 Mail Service Center Raleigh, North Carolina 27699-1617 EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 9 of 22 FACILITY NAME AND PERMIT NUMBER. Benson WWTP, NCO020389 PERMIT ACTION REQUESTED Renewal RIVER BASIN: Neuse SUPPLEMENTAL APPLICATION INFORMATION PART D. EXPANDED EFFLUENT TESTING DATA Refer to the directions on the cover page to determine whether this section applies to the treatment works Effluent Testing 1.0 mgd and Pretreatment Works If the treatment works has a design flow greater than or equal to 10 mgd or it has (or is required to have) a pretreatment program, or is otherwise required by the permitting authority to provide the data, then provide effluent testing data for the following pollutants Provide the indicated effluent testing information and any other information required by the permitting authority for each outfall through which effluent is discharged Do not include information on combined sewer overflows in this section All information reported must be based on data collected through analyses conducted using 40 CFR Part 136 methods In addition, these data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136 Indicate in the blank rows provided below any data you may have on pollutants not specifically listed in this form At a minimum, effluent testing data must be based on at least three pollutant scans and must be no more than four and one-half years old Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States) POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD MUMDL Conc. Units Mass Units Conc. Units Mass Units Number of Samples METALS (TOTAL RECOVERABLE), CYANIDE, PHENOLS, AND HARDNESS ANTIMONY 40 pg/L 28 pg/L 2 EPA204 2 50 ARSENIC <5 0 pg/L <5 0 pg/L 2 EPA206 2 50 BERYLLIUM <1 0 pg/L <1 0 pg/L 2 EPA200 7 1 0 CADMIUM <1 0 pg/L <1 0 pg/L 2 EPA213 2 1 0 CHROMIUM <5 0 pg/L <5 0 pg/L 2 EPA200 7 50 COPPER <10 pg/L <10 pg/L 2 EPA2201 10 LEAD <50 pg/L <5 0 pg/L 2 EPA239 2 50 MERCURY <0 001 pg/L <0 001 pg/L 2 EPA1631 0 001 NICKEL <10 pg/L <10 pg/L 2 EPA200 7 10 SELENIUM <10 pg/L <10 pg/L 2 EPA270 2 10 SILVER <50 pg/L <5 0 pg/L 2 EPA200 7 50 THALLIUM <1 0 pg/L <1 0 pg/L 2 EPA279 2 1 0 ZINC 44 pg/L 43 pg/L 2 EPA2891 2 CYANIDE <0 005 mg/L <0 005 mg/L 2 SM450OCNE 0 005 TOTAL PHENOLIC COMPOUNDS <5 pg/L <5 pg/L 2 SM510A&B <2 HARDNESS (as CaCO3) 53 mg/L 44 mg/L 2 SM2340C Use this space (or a separate sheet) to provide information on other metals requested by the permit writer EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 10 of 22 FACILITY NAME AND PERMIT NUMBER: Benson WWTP, NCO020389 PERMIT ACTION REQUESTED: Renewal RIVER BASIN. Neuse Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States ) POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD ML/MDL Cone. Units Mass Units Cone. Units Mass Units Number of Samples VOLATILE ORGANIC COMPOUNDS ACROLEIN <100.00 pg/L <100 00 pg/L 2 EPA624 100 ACRYLONITRILE <50 00 pg/L <50.00 pg/L 2 EPA624 50 BENZENE <5.00 pg/L <5.00 pg/L 2 EPA624 5 BROMOFORM <5 00 pg/L <5.00 pg/L 2 EPA624 5 CARBON TETRACHLORIDE <5.00 pg/L <5.00 pg/L 2 EPA624 5 CHLOROBENZENE <5.00 pg/L <5 00 pg/L 2 EPA624 5 CHLORODIBROMO- METHANE <5.00 pg/L <5.00 pg/L 2 EPA624 5 CHLOROETHANE <10 00 pg/L <10.00 pg/L 2 EPA624 10 2-CHLOROETHYLVINYL ETHER <5.00 pg/L <5.00 pg/L 2 EPA624 5 CHLOROFORM <5.00 pg/L <5.00 pg/L 2 EPA624 5 DICHLOROBROMO- METHANE <5.00 pg/L <5.00 pg/L 2 EPA624 5 1,1-DICHLOROETHANE <5.00 pg/L <5.00 pg/L 2 EPA624 5 1,2-DICHLOROETHANE TRANS-I,2-DICHLORO- ETHYLENE <5.00 <5 00 pg/L pg/L <5.00 <5.00 pg/L pg/L 2 2 EPA624 EPA624 5 5 1,1-DICHLORO- ETHYLENE <5.00 pg/L <5.00 pg/L 2 EPA624 5 1,2-DICHLOROPROPANE <5.00 pg/L <5.00 pg/L 2 EPA624 5 1,3-DICHLORO- PROPYLENE <5 00 pg/L <5.00 pg/L 2 EPA624 5 ETHYLBENZENE <5.00 pg/L <5 00 pg/L 2 EPA624 5 METHYL BROMIDE <10 00 pg/L <10 00 pg/L 2 EPA624 10 METHYL CHLORIDE <10 00 pg/L <10.00 pg/L 2 EPA624 10 METHYLENE CHLORIDE <10.00 pg/L <10 00 pg/L 2 EPA624 10 1,1,2,2 -TETRA- CHLOROETHANE <5 00 pg/L <5.00 pg/L 2 EPA624 5 TETRACHLORO- ETHYLENE <5 00 pg/L <5 00 pg/L 2 EPA624 5 TOLUENE <5 00 pg/L <5 00 pg/L 2 EPA624 5 EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 11 of 22 FACILITY NAME AND PERMIT NUMBER: Benson WWTP, NCO020389 PERMIT ACTION REQUESTED- Renewal RIVER BASIN Neuse Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States ) POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD ML/MDL Conc. Units Mass Units Conc. Units Mass Units Number of Samples TRICHLOROETHANE <5.00 pg/L <5 00 pg/L 2 EPA624 5 1.1'2 TRICHLOROETHANE <5.00 pg/L <5.00 pg/L 2 EPA624 5 TRICHLOROETHYLENE <5 00 pg/L <5 00 pg/L 2 EPA624 5 VINYL CHLORIDE <10.00 pg/L <10 00 pg/L 2 EPA624 10 Use this space (or a separate sheet) to provide information on other volatile organic compounds requested by the permit writer ACID -EXTRACTABLE COMPOUNDS P -CHLORO -M -CRESOL <20 00 pg/L <20 00 pg/L 2 EPA625 20 2 -CHLOROPHENOL <10.00 pg/L <10.00 pg/L 2 EPA625 10 2,4-DICHLOROPHENOL <10.00 pg/L <10.00 pg/L 2 EPA625 10 2,4 -DIMETHYLPHENOL <10.00 pg/L <10.00 pg/L 2 EPA625 10 4,6-DINITRO-0-CRESOL <50 00 pg/L <50.00 pg/L 2 EPA625 50 2,4-DINITROPHENOL <50.00 pg/L <15.00 pg/L 2 EPA625 50 2-NITROPHENOL <10.00 pg/L <10.00 pg/L 2 EPA625 10 4-NITROPHENOL <50.00 pg/L <50.00 pg/L 2 EPA625 50 PENTACHLOROPHENOL <50.00 pg/L <50.00 pg/L 2 EPA625 50 PHENOL <2.00 pg/L <2.00pg/L 2 EPA625 10 2,4,6- TRICHLOROPHENOL <10.00 pg/L <10.00 pg/L 2 EPA625 10 Use this space (or a separate sheet) to provide information on other acid -extractable compounds requested by the permit writer BASE -NEUTRAL COMPOUNDS ACENAPHTHENE <10 00 pg/L <10.00 pg/L 2 EPA625 10 ACENAPHTHYLENE <10 00 pg/L <10.00 pg/L 2 EPA625 10 ANTHRACENE <10 00 pg/L <10 00 pg/L 2 EPA625 10 BENZIDINE <100 00 pg/L <100 00 pg/L 2 EPA625 100 BENZO(A)ANTHRACENE <10 00 pg/L <10 00 pg/L 2 EPA625 10 BENZO(A)PYRENE <10 00 pg/L <10 00 pg/L 2 EPA625 10 EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 12 of 22 FACILITY NAME AND PERMIT NUMBER Benson WWTP, NCO020389 PERMIT ACTION REQUESTED: Renewal RIVER BASIN Neuse Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States ) POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD ML/MDL Conc. Units Mass Units Conc. Units Mass Units Number of Samples 3,4 BENZO- FLUORANTHENE <10.00 pg/L <10.00 pg/L 2 EPA625 10 BENZO(GHI)PERYLENE <10 00 pg/L <10 00 pg/L 2 EPA625 10 BENZO(K) FLUORANTHENE <10 00 pg/L <10 00 pg/L 2 EPA625 10 BIS (2-CHLOROETHOXY) METHANE <10.00 pg/L <10.00 pg/L 2 EPA625 10 BIS (2-CHLOROETHYL)- ETHER <10 00 pg/L <10 00 pg/L 2 EPA625 10 BIS (2-CHLOROISO- PROPYL)ETHER <10.00 pg/L <10.00 pg/L 2 EPA625 10 BIS (2-ETHYLHEXYL) PHTHALATE <20 00 pg/L <20.00 pg/L 2 EPA625 20 4-BROMOPHENYL PHENYLETHER <10.00 pg/L <10.00 pg/L 2 EPA625 10 BUTYL BENZYL PHTHALATE <10 00 pg/L <10.00 pg/L 2 EPA625 10 2 -CHLORO- NAPHTHALENE <10.00 pg/L <10.00 pg/L 2 EPA625 10 4-CHLORPHENYL PHENYLETHER <10.00 pg/L <10.00 pg/L 2 EPA625 10 CHRYSENE <10.00 pg/L <10.00 pg/L 2 EPA625 10 DI -N -BUTYL PHTHALATE <10.00 pg/L <10.00 pg/L 2 EPA625 10 DI-N-OCTYL PHTHALATE <10.00 pg/L <10.00 pg/L 2 EPA625 10 DIBENZO(A,H) ANTHRACENE <10.00 pg/L <10.00 pg/L 2 EPA625 10 1,2 -DICHLOROBENZENE <10.00 pg/L <10.00 pg/L 2 EPA625 10 1,3 -DICHLOROBENZENE <10.00 pg/L <10 00 pg/L 2 EPA625 10 1,4 -DICHLOROBENZENE <10 00 pg/L <10.00 pg/L 2 EPA625 10 3,3-DICHLORO- BENZIDINE <10.00 pg/L <10.00 pg/L 2 EPA625 10 DIETHYL PHTHALATE <10 00 pg/L <10.00 pg/L 2 EPA625 10 DIMETHYL PHTHALATE <10 00 pg/L <10 00 pg/L 2 EPA625 10 2,4-DINITROTOLUENE <10.00 pg/L <10 00 pg/L 2 EPA625 10 2,6-DINITROTOLUENE <10 00 pg/L <10 00 pg/L 2 EPA625 10 1,2 -DIPHENYL- HYDRAZINE <10 00 pg/L <10 00 pg/L 2 EPA625 10 EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 13 of 22 FACILITY NAME AND PERMIT NUMBER: Benson WWTP, NCO020389 PERMIT ACTION REQUESTED Renewal RIVER BASIN Neuse Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States ) POLLUTANTNumber MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD ML/MDL Conc. Units Mass Units Conc. Unit Mass Units of Samples FLUORANTHENE <10 00 pg/L <10 00 pg/L 2 EPA625 10 FLUORENE <10 00 pg/L <10.00 pg/L 2 EPA625 10 HEXACHLOROBENZENE <10.00 pg/L <10 00 pg/L 2 EPA625 10 HEXACHLORO- BUTADIENE <10 00 pg/L <10.00 pg/L 2 EPA625 10 HEXACHLOROCYCLO- PENTADIENE <10.00 pg/L <10 00 pg/L 2 EPA625 10 HEXACHLOROETHANE <10 00 pg/L <10 00 pg/L 2 EPA625 10 INDENO(1,2,3-CD) PYRENE <10 00 pg/L <10 00 pg/L 2 EPA625 10 ISOPHORONE <10 00 pg/L <10 00 pg/L 2 EPA625 10 NAPHTHALENE <10.00 pg/L <10 00 pg/L 2 EPA625 10 NITROBENZENE <10 00 pg/L <10.00 pg/L 2 EPA625 10 N-NITROSODI-N- PROPYLAMINE <10 00 pg/L <10 00 pg/L 2 EPA625 10 N-NITROSODI- METHYLAMINE <10 00 pg/L <10 00 pg/L 2 EPA625 10 N-NITROSODI- PHENYLAMINE <10 00 pg/L <10 00 pg/L 2 EPA625 10 PHENANTHRENE <10 00 pg/L <10 00 pg/L 2 EPA625 10 PYRENE <10.00 pg/L <10 00 pg/L 2 EPA625 10 1,2,4- TRICHLOROBENZENE <10 00 pg/L <10 00 pg/L 2 EPA625 10 Use this space (or a separate sheet) to provide information on other base -neutral compounds requested by the permit writer Use this space (or a separate sheet) to provide information on other pollutants (e g , pesticides) requested by the permit writer END OF PART D. REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS OF FORM 2A YOU MUST COMPLETE EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 14 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED RIVER BASIN - Benson WWTP, NCO020389 Renewal Neuse SUPPLEMENTAL APPLICATION INFORMATION PART E. TOXICITY TESTING DATA POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the facility's discharge points 1) POTWs with a design flow rate greater than or equal to 1 0 mgd, 2) POTWs with a pretreatment program (or those that are required to have one under 40 CFR Part 403), or 3) POTWs required by the permitting authority to submit data for these parameters • At a minimum, these results must include quarterly testing for a 12 -month period within the past 1 year using multiple species (minimum of two species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution Do not include information on combined sewer overflows in this section All information reported must be based on data collected through analysis conducted using 40 CFR Part 136 methods In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136 • In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years If a whole effluent toxicity test conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a toxicity reduction evaluation, if one was conducted • If you have already submitted any of the information requested in Part E, you need not submit it again Rather, provide the information requested in question E 4 for previously submitted information If EPA methods were not used, report the reasons for using alternate methods If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E If no biomomtormg data is required, do not complete Part E Refer to the Application Overview for directions on which other sections of the form to complete EA Required Tests. Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years ® chronic ❑ acute E 2 Individual Test Data Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years Allow one column per test (where each species constitutes a test) Copy this page if more than three tests are being reported Test number Test number Test number a Test information Test species & test method number Ceriodaphnia dubia Pimephales promelas Age at initiation of test Outfall number 001 001 Dates sample collected Date test started Duration 7 -Day Test b Give toxicity test methods followed Manual tale Method 1002 0 Method 1000 0 Edition number and year of publication 4th Edition, October 2002 Ott' Edition, October 2002 Page number(s) Pg. 141 —196 Pg 53-111 c Give the sample collection method(s) used For multiple grab samples, indicate the number of grab samples used 24 -Hour composite X X Grab d Indicate where the sample was taken in relation to disinfection (Check all that apply for each Before disinfection After disinfection After dechlorination X X EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 15 of 22 FACILITY NAME AND PERMIT NUMBER: Benson WWTP, NCO020389 PERMIT ACTION REQUESTED Renewal RIVER BASIN. Neuse Test number: Test number, Test number: e Describe the point in the treatment process at which the sample was collected Sample was collected Effluent Effluent f For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both Chronic toxicity X X Acute toxicity g Provide the type of test performed Static Static -renewal X X Flow-through h Source of dilution water If laboratory water, specify type, if receiving water, specify source Laboratory water Soft Synthetic Receiving water Lake Brandt I Type of dilution water If saltwater, specify "natural' or type of artificial sea salts or brine used Fresh water X X Salt water 1 Give the percentage effluent used for all concentrations in the test series k Parameters measured during the test (State whether parameter meets test method specifications) pH Y Salinity Temperature Y Ammonia Dissolved oxygen Y I Test Results Acute Percent survival in 100% effluent Not Tested Not Tested % LC50 Not Tested Not Tested 95% C I Not Tested Not Tested % Control percent survival Not Tested Not Tested % Other (describe) Not Tested Not Tested EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 16 of 22 FACILITY NAME AND PERMIT NUMBER Benson WWTP NCO020389 PERMIT ACTION REQUESTED- Renewa RIVER BASIN. Neuse Chronic NOEC Not Tested Not Tested % IC25 Not Tested Not Tested % Control percent survival Not Tested Not Tested % Other (describe) Not Tested Not Tested m Quality Control/Quality Assurance Is reference toxicant data available? Was reference toxicant test within acceptable bounds? What date was reference toxicant test run (MM/DD/YYYY)? — — — — — — Other (describe) E 3. Toxicity Reduction Evaluation Is the treatment works involved in a Toxicity Reduction Evaluation? ❑ Yes ® No If yes, describe E4 Summary of Submitted Biomomtonng Test Information If you have submitted biomomtoring test information, or information regarding the cause of toxicity, within the past four and one-half years, provide the dates the information was submitted to the permitting authority and a summary of the results Date submitted / / (MM/DD/YYYY) Summary of results (see instructions) All toxlcltV results reports have pre ous y been submitted to NCDEQ See Append x for testing summary and dates END OF PART E. ,REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS, OF FORM 2A YOU MUST COMPLETE. EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 17 of 22 FACILITY NAME AND PERMIT NUMBER PERMIT ACTION REQUESTED. RIVER BASIN - Benson WWTP NCO020389 Renewa Neuse SUPPLEMENTAL APPLICATION INFORMATION PART F. INDUSTRIAL USER DISCHARGES AND RCRAICERCLA WASTES All treatment works receiving discharges from significant industrial users or which receive RCRA,CERCLA, or other remedial wastes must complete part F GENERAL INFORMATION: Fl. Pretreatment program Does the treatment works have, or is subject ot, an approved pretreatment program? ® Yes ❑ No F2 Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs) Provide the number of each of the following types of industrial users that discharge to the treatment works a Number of non-categoncal SIUs b Number of CIUs 0 SIGNIFICANT INDUSTRIAL USER INFORMATION: Supply the following information for each SIU If more than one SIU discharges to the treatment works, copy questions F 3 through F 8 and provide the information requested for each SIU. F3. Significant Industrial User Information Provide the name and address of each SIU discharging to the treatment works Submit additional pages as necessary Name Chicopee, Inc d b a Berry Plastics Mailing Address 1403 Chicopee Road Benson, NC 27504 F 4 Industrial Processes Describe all the industrial processes that affect or contribute to the SIU's discharge Manufacturing of non -woven textile products F 5 Principal Product(s) and Raw Material(s) Describe all of the principal processes and raw materials that affect or contribute to the Sl U's discharge Principal product(s) Non -woven roll goods for medical end use and industrial end use Raw material(s) Staple fibers, surfactants, pigments, anti -foams, pH adjuster, thickeners F 6 Flow Rate. a Process wastewater flow rate Indicate the average daily volume of process wastewater discharge into the collection system in gallons per day (gpd) and whether the discharge is continuous or intermittent 320,000 gpd (x continuous or intermittent) b Non -process wastewater flow rate Indicate the average daily volume of non -process wastewater flow discharged into the collection system in gallons per day (gpd) and whether the discharge is continuous or intermittent 149,000 gpd (x continuous or intermittent) F7 Pretreatment Standards. Indicate whether the SIU is subject to the following a Local limits ® Yes ❑ No b Categorical pretreatment standards ❑ Yes ® No If subject to categorical pretreatment standards, which category and subcategory? n/a EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 18 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED' RIVER BASIN - Benson WWTP, NCO020389 Renewal Meuse F 8 Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e g, upsets, interference) at the treatment works in the past three years? ❑ Yes ® No If yes, describe each episode n/a RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE: F.9 RCRA Waste. Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rad or dedicated pipe? ❑ Yes 0 No (go to F 12) F 10. Waste transport Method by which RCRA waste is received (check all that apply) ❑ Truck ❑ Rad ❑ Dedicated Pipe F 11 Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units) EPA Hazardous Waste Number Amount Units n/a n/a n/a n/a n/a n/a n/a n/a n/a CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER: F.12 Remediation Waste Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities? ❑ Yes (complete F 13 through F 15 ) ® No F.13 Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to originate in the next five years) n/a F 14. Pollutants List the hazardous constituents that are received (or are expected to be received) Include data on volume and concentration, if known (Attach additional sheets if necessary ) n/a F.15 Waste Treatment. a Is this waste treated (or will be treated) prior to entering the treatment works? ❑ Yes ❑ No If yes, describe the treatment (provide information about the removal efficiency) n/a b Is the discharge (or will the discharge be) continuous or intermittent? ❑ Continuous ❑ Intermittent If intermittent, describe discharge schedule n/a 1h0. �r�P,, . -T . S � , ~' R i �•l � ^ y !cr-^ G , <.-S. a.v 3n 91 yr4c �� ,'--+i31•�`�, ". J_!I���•s'. : �.,^�,S `-�,�"•�•-. ' � _ ' ,5�'�,.✓i�r ^S-�, .-s.a , ._ -_ -1 arn�� -` - -�'•' ^~: A , ,0P, PA. T ` - i �Y R^.J d'.s+b'�+ 'CZ b' eT ,y FTSE ��:f�r �` ;nM+ ^i gyp' 1� ■ P. Yi � �t S.. � �� rrRe �.3_ F'�1 'I ~' jo. TIDE APL��l���n11t���[���IE�IV t�Af�� 't��iO"D�TE[�I�II�tIE �i�„ ��f OTII�IF�`l�'��''_: , a-}I:wj�1�U $,�_ �;-l-tiS.y,, y/.,`.^ ♦T n'--i1�« .tlxa•1� z.{:- .� 3 t roH �,� -t.. �,�,;��'-�`.-.i�- O�IilVl �P►��..��t.+�` t��5iG y P'L�TE, "-a,-�. EPA Form 3510-2A (Rev 1-99) Replaces EPA forms 7550-6 & 7550-22 Page 19 of 22 Topographic Map t6a � • y • .r air, 7 INFLUENT INTfRCHAl�i(`>r 3?8 f s. / Lono x • Gree • - r f . EFFLUENT • SLUDGE AERATION BASIN • EQ BASIN w► �* f ; �f , DISCHARGE POINT _ rits 00 .11071 em Town of Benson WWTP NPDES - Permit No. NCO020389 Legend W S E Ex. Road Feet 0 1,200 Topographic MapEx. Pipe TV Ex. Structure Town of Benson "Topographic Source: USGS Johnston County, NC THEWOOTENCOMPANv F6G111FE 6MG .i.6 A6f nilEG* < 1 x • Gree • - r f . EFFLUENT • SLUDGE AERATION BASIN • EQ BASIN w► �* f ; �f , DISCHARGE POINT _ rits 00 .11071 em Town of Benson WWTP NPDES - Permit No. NCO020389 Legend W S E Ex. Road Feet 0 1,200 Topographic MapEx. Pipe TV Ex. Structure Town of Benson "Topographic Source: USGS Johnston County, NC THEWOOTENCOMPANv F6G111FE 6MG .i.6 A6f nilEG* Process Flow Diagram 97 FLOW BALANCE NUMBER MARCH 2013 ' i TOWN OF BENSON i NORTH CAROLINA i WWTP EXPANSION PRELIMINARY DESIGN THE WOOTEN COMPANY SSS ISIJO W,bwiF 015 lroene l4ptOv F °IIS rE" 197 arau�nr°urfua. -------- ------ -----------— -- frturvlrs.url+l°ar�n°II 0011 IIS J X111 lil --.2 --w, iI II L-- 025 ------ wou wu—.----- — — — — ------------� � II low SCHEMATIC OF EXPANDED BENSON WASTEWATER TREATMENT FACILITY (1.97 MGD) Sludge Management Plan Sludge Management Plan Benson Wastewater Treatment Plant NPDES Permit No. NC0020389 Non -Discharge Permit No. WQ0012514 The Town of Benson operates a 1.9 mgd modified biological nutrient removal (BNR) plant which discharges its effluent into Hannah Creek in the Neuse River Basin under NPDES Permit No. NC0020389. Waste sludge from the modified BNR plant is discharged to the existing 3,247,000 gallon capacity aerated basin for long-term sludge stabilization and storage. Periodically, the sludge is allowed to settle in the basin and the supernatant is withdrawn and recycled back to the plant headworks for reprocessing. The stabilized sludge which meets pollutant limits and Class B pathogen reduction and vector attraction reduction requirements is later disposed of in accordance with Non -Discharge Permit No. WQ0012514. The Class B pathogen reduction requirements are met using either Alternative 1 [Monitoring of Fecal Coliform, 503.32(b)(2)] or Alternative 2 [Use of PSRP, 503.32(b)(3)]. The vector attraction reduction requirements are met using Option 3 [503.33(b)(3)], Option 6 [503.33(b)(6)], Option 9 [503.33(b)(9)], or Option 10 [503.33(b)(10)]. Priority Pollutant Analysis Annual Monitoring and Pollutant Scan Permit No. 0020389 Outfa11001 Facility Name Town of Benson WWTP ORC Martin Morris Date of sampling 10/1411 Phone __-_ 919-894-2373 Analytical Laboratory Environment One Month October Year 201$ Form - DMR- PPA -1 page I Parameter Sample Type Analytical Quantitation Method Level Sample Result Units of Measurement Number of samples Ammonia (as N) Composite SM4500HN3 0.32 mg/1 1 Dissolved oxygen Composite SM4500 OG 8.40 mg/l 1 Nitrate/Nitrite Composite EPA353.2 2.19 mg/l 1 Total Kjeldahl nitrogen Composite EPA351.2 0.64 mg/1 1 Total Phosphorus Composite SM4500P 1.55 mg/I 1 Total dissolved solids Composite EPA160.1 196 mg/l 1 Hardness Composite SM23400 351 mg/l 1 Chlorine (total residual, TRC) Grab SM4500CLE <10.00 ug/1 1 Oil and grease Grab SM5520B <5.0 mg/1 1 Metals (total recoverable), cyanide and total phenols Antimony Composite EPA204.2 <3.0 ug/I 1 Arsenic Composite EPA206.2 <5.0 ug/I 1 Beryllium Composite EPA200.7 <1.0 ug/1 1 Cadmium Composite EPA213.2 <1.0 ug/I 1 Chromium Composite EPA200.7 <5.0 ug/1 I Copper Composite EPA220.1 <10 ug/l 1 Lead Composite EPA239.2 <5.0 ug/1 1 Mercury Composite EPA1631 < 0.001 ug/I 1 Nickel Composite EPA200.7 <10 ug/1 1 Selenium Composite EPA270.2 <10 ug/I I Silver Composite EPA200.7 <5.0 ug/1 1 Thallium Composite EPA279.2 <1.0 ug/I 1 Zinc Composite IEPA289.1 1 44 ug/l 1 Cyanide Grab SM450OCNE <0.005 mg/1 1 Total phenolic compounds Grab SM51OA&B <51 ug/1 1 Volatile organic compounds Acrolein Grab EPA624 <100.00 ug/1 1 Acrylonitrile Grab EPA624 <50.00 ug/1 1 Benzene Grab EPA624 <5.0 ug/I I Bromoform Grab EPA624 <5.0 ug/I 1 Carbon tetrachloride Grab EPA624 <5.0 ug/1 1 Chlorobenzene Grab EPA624 <5.0 ug/I 1 Chlorodibromomethane Grab EPA624 <5.00 ug/1 1 Chloroethane Grab EPA624 <10.00 ug/1 1 2-chioroethylvinyl ether Grab EPA624 <5.00 ug/1 1 Chloroform Grab EPA624 <5.00 ug/i 1 Dichlorobromomethane Grab EPA624 <5.00 ug/I 1 1,1-Wehloroethane Grab EPA624 <5.00 ug/1 1 1,2-dichloroethane Grab EPA624 <5.00 ug/1 1 Trans-1,2-dichloroetlhylene Grab EPA624 <5.00 ug/1 1 Form - DMR- PPA -1 page I Permit No. 0020389 Annual Monitoring and Pollutant Scan Outfall 001 Month October Year 9M 1 Form - DMR- PPA -1 Page 2 Parameter Sample Type Analytical Method Quantitation Level Sample Result Units of Measurement Number of samples Volatile organic compounds (Cont.) 1,1-dichloroethylene Grab EPA624 <5.00 ug/1 I 1,2-dichloropropane Grab EPA624 <5.00 ug/l 1 1,3-dichloropropylene Grab EPA624 <5.00 ug/l 1 Ethyibenzene Grab EPA624 <5.00 ug/l 1 Methyl bromide Grab EPA624 <10.00 ug/1 1 Methyl chlonde Grab EPA624 <10.00 ug/I I Methylene chloride Grab EAP624 <10.00 ug/I 1 1,1,2,2 -tetrachloroethane Grab EPA624 <5.00 ug/1 I Tetrachloroethylene Grab EPA624 <5.00 ug/1 I Toluene Grab EPA624 <5.00 ug/I 1 1,1,1 -trichloroethane Grab EPA624 <5.00 ug/1 I 1,1,2 -trichloroethane Grab EPA624 <5.00 ug/I 1 Trichloroethylene Grab EPA624 <5.00 ug/l 1 Vinyl chloride Grab EPA624 <10.00 ug/1 1 Acid -extractable compounds P-chloro-m-creso Grab EPA625 <20.00 ug/I i 2 -chlorophenol Grab EPA625 <10 00 ug/l 1 2,4-dichlorophenoi Grab EPA625 <10.00 ug/l 1 2,4-dunethylphenol Grab EPA625 <10 00 ug/1 1 4,6-dinitro-o-cresol Grab EPA625 <50.00 ug/l 1 2,4-drnitrophenol Grab EPA625 <50.00 ug/1 1 2-nitrophenol Grab EPA625 <10.00 ug/l 1 4-mtrophenol Grab EPA625 <50.00 ug/I 1 Pentachlorophenol Grab EPA625 <50A0 ug/1 1 Phenol Grab EPA625 <2.00 ug/1 I 2,4,6-tnchlorophenoi Grab EPA625 <10.00 ug/1 1 Base -neutral compounds Acenaphthene Grab EPA625 <10.00 ug /I 1 Acenaphthylene Grab EPA625 <10.00 ug/l 1 Anthracene Grab EPA625 <10.00 ug/l 1 Benzidine Grab EPA625 <100 00 ug/l 1 Benzo(a)anthracene Grab EPA625 <10.00 ug/1 1 Benzo(a)pyrene Grab EPA625 <10.00 ug/l 1 3,4 benzofluoranthene Grab EPA625 <10 00 ug/l 1 Benzo(ghi)perylene Grab EPA625 <10.00 ug/1 I Benzo(k)fluoranthene Grab EPA625 <10.00 ug/l 1 Bis (2-chloroethoxy) methane Grab EPA625 <10 001 ug/l 1 Bis (2-chloroethyl) ether Grab EPA625 <10.00 ug/I 1 Bis (2-chloroisopropyl) ether Grab EPA625 <10.00 ug/l 1 Bis (2-ethylhexyl) phthalate Grab EPA625 <20.00 ug/i 1 4-bromophenyl phenyl ether Grab EPA625 <10.00 ug/l 1 Butyl benzyl phthalate Grab EPA625 <10.00 ug/I 1 2-chloronaphthalene Grab EPA625 <10.0ol uu/] r Form - DMR- PPA -1 Page 2 Annual Monitoring and Pollutant Scan Permit No. 0020389 Month October Outfall 001 Year 2011 I certify under penalty of law that this document and all attachments were prepared under my direction and supervision in accordance with a system to design to assure that qualified perdonnel properly gather and evaluat the information submitted. Based on my inquiry of the person or persons that manage the system, or those persons directly responsibel for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate and complete. 1 rim aware that there are significant penalties for submitting false information, including the possibility of fines and imprisonment for knowing violations. Matt Zapp Authorized Representative name — � Signa e Date Form - DMR- PPA -1 Page 3 4-chlorophenyl phenyl ether Grab EPA625 <10.00 ug/l 1 Parameter Sample Type Analytical Method Quantitation Level Sample Result Units of Measurement Number of samples Base -central compounds {coat.) Chrysene Grab EPA625 <10.00 ug/i 1 Di -n -butyl phthalate Grab EPA625 <10.00 ug/l 1 Di-n-octyl phthalate Grab EPA625 <10.00 ug/1 1 Dibenw(a,h)anthracene Grab EPA625 <10.00 ug/l 1 1,2 -dichlorobenzene Grab EPA625 <10.00 ug/l 1 1,3 -dichlorobenzene Grab EPA625 <10.Q0 ug/l I 1,4 -dichlorobenzene Grab EPA625 <10.00 ug/1 1 3,3-dichlorobenzidine Grab EPA625 <10.00 ug/l I Diethyl phthalate Grab EPA625 <10.00 ug/l 1 Dimethyl phthalate Grab EPA625 <10.00 ug/l 1 2,4-dinitrotoluene Grab EPA625 <10.00 ug/l 1 2,6-dinitrotoluene Grab EPA625 <10.00 ug/l 1 1,2-dipheny1hydrazine Grab EPA625 <10.00 ug/l 1 Fluoranthene Grab EPA625 <10.00 ug/l 1 Fluorene Grab EPA625 <10.00 ug/l 1 Hexachlorobenzene Grab EPA625 <10.00 ug/Z 1 Hexachlorobutadtene Grab EPA625 <10.00 ug/l 1 Hexachlorocyclo-pentadiene Grab EPA625 <10.00 ug/l 1 Hexachloroethane Grab EPA625 <10.00 ug/i I Indeno(1,2,3-cd)pyrene Grab EPA625 <10.00 ug/l 1 Isophorone I Grab EPA625 <10.00 ug/l I Naphthalene Grab EPA625 <10.00 ug/l 1 Nitrobenzene Grab EPA625 <10.00 ug/l 1 N-nitrosodi-n-propylamine Grab EPA625 <10.00 ug/l I N-nitrosodimethylamine Grab EPA625 <10.00 ug/l 1 N-nitrosodiphenylamine Grab EPA625 <10.00 ug/l 1 Phenanthrene Grab EPA625 <10.00 ug/l 1 Pyrene Grab EPA625 <10.00 ug/l 1 1,2,4,-trichlorobenzene Grab EPA625 <10.00 vg/1 I I certify under penalty of law that this document and all attachments were prepared under my direction and supervision in accordance with a system to design to assure that qualified perdonnel properly gather and evaluat the information submitted. Based on my inquiry of the person or persons that manage the system, or those persons directly responsibel for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate and complete. 1 rim aware that there are significant penalties for submitting false information, including the possibility of fines and imprisonment for knowing violations. Matt Zapp Authorized Representative name — � Signa e Date Form - DMR- PPA -1 Page 3 Permit No. 0020389 Outfall 001 Facility Name Date of sampling ____ Analytical Laboratory Annual Monitoring and Pollutant Scan Town of Benson WWTP ORC Martin Morris 7/21/14 Phone - 919-894-2373 Environment One Month OeWbCl' ` Year 201q Form - DMR- FPA -1 Page 1 Parameter Sample Type Analytical Quantitation Method Level Sample Result Units of Measurement Number of samples Ammonia (as N) Composite SM4500HN3 0.29 mg/l 1 Dissolved oxygen Composite SM4500 OG 7.90 mg/l 1 Nitrate/Nitrite Composite EPA353.2 6.37 mg/l 1 Total Kjeldahl nitrogen Composite EPA351.2 1.18 mg/l 1 Total Phosphorus Composite SM4500P 1.45 mg/l 1 Total dissolved solids Composite EPA160.1 298 mg/l 1 Hardness Composite SM2340C 53 mg/l 1 Chlorine (total residual, TRC) Grab SM4500CLE <10.00 ng/l 1 Oil and grease Grab SM5520B 1<5.0 mg/l 1 Metals (total recoverable), cyanide and total phenols Antimony Composite EPA204.2 4 ug/l 1 Arsenic Composite EPA206.2 <5.0 ug/l 1 Beryllium Composite EPA200.7 <1.0 ug/1 1 Cadmium Composite EPA213.2 <1.0 ug/l 1 Chromium Composite EPA200.7 <5.0 ug/l 1 Copper Composite EPA220.1 <10 ug/l 1 Lead Composite EPA239.2 <5.0 ug/l 1 = Mercury- -Composite- EP-A-1-63-1-- PE-1631-Nickel Nickel Composite EPA200.7 <10 ug/1 1 Selenium Composite EPA270.2 <10 ug/1 1 Silver Composite EPA200.7 <5.0 ug/l 1 Thallium Composite EPA279.2 <1.0 ug/I 1 Zinc Composite EPA289.1 42 ug/1 1 Cyanide Grab SM450OCNE <0.005 mg/I 1 Total phenolic compounds Grab SM510A&B <5 ug/I 1 Volatile organic compounds Acrolein Grab EPA624 <100.00 ug/l 1 Acrylonitrile Grab EPA624 <50.00 ug/1 1 Benzene Grab EPA624 <5.0 ug/l 1 Bromoform Grab EPA624 <5.0 ug/i 1 Carbon tetrachloride Grab EPA624 <5.0 ug/l 1 Chlorobenzene Grab EPA624 <5.0 ug/1 1 Chlorodibromomethane Grab EPA624 <5.00 ug/l 1 Chloroethane Grab EPA624 <10.00 ug/I 1 2-chloroethylvinyl ether Grab EPA624 <5.00 ug/l 1 Chloroform Grab EPA624 <5.00 ug/I 1 Dichlorobromomethane Grab EPA624 <5.00 ug/1 1 1,1-dichloroethane Grab EPA624 <5.00 ug/1 1 1,2-dichloroethane Grab EPA624 <5.00 ug/l 1 Trans-1,2-dichloroethylene Grab IEPA624 I I <5.00 ug/l 1 Form - DMR- FPA -1 Page 1 Annual Monitoring and Pollutant Scan Permit No. 0020389 Month. 1-t%er Outfall 001 Year 201 J Form - DMR- PPA -1 Page 2 Parameter Sample Type Analytical Method Quantitation Level Sample Result Units of Measurement Number of samples Volatile organic compounds (Cont.) 1,1-dichloroethylene Grab EPA624 <5.00 ug/l 1 1,2-dichloropropane Grab EPA624 <5.00 ug/l 1 1,3-dichloropropylene Grab EPA624 <5.00 ug/I 1 Ethylbenzene Grab EPA624 <5.00 ug/l 1 Methyl bromide Grab EPA624 <10.00 ug/1 1 Methyl chloride Grab EPA624 <10.00 ug/I 1 Methylene chloride Grab EAP624 <10.00 ug/l 1 1,1,2,2 -tetrachloroethane Grab EPA624 <5.00 ug/1 1 Tetrachloroethylene Grab EPA624 <5.00 ug/l 1 Toluene Grab EPA624 <5.00 ug/l 1 1,1,1 -trichloroethane Grab EPA624 <5.00 ug/l 1 1,1,2 -trichloroethane Grab EPA624 <5.00 ug/I 1 Trichloroethylene Grab EPA624 <5.00 ug/l 1 Vinyl chloride I Grab IEPA624 <10.00 ug/1 1 Acid -extractable compounds P-chloro-m-creso Grab EPA625 <20.00 ug/1 1 2 -chlorophenol Grab EPA625 <10.00 ug/l 1 2,4-dichlorophenol Grab EPA625 <10.00 ug/l 1 2,4 -dimethylphenol Grab EPA625 <10.00 ug/1 1 4,6-dimtro-o-cresol Grab EPA625 <50.00 ug/1 1 2,4-dinitrophenol Grab EPA625 <50.00 ug/1 _ 1 2-nitrophenol Grab EPA625 <10.00 ug/l 1 4-nitrophenol Grab EPA625 <50.00 ug/l 1 Pentachlorophenol Grab EPA625 <50.00 ug/1 1 Phenol Grab EPA625 <2,00 ug/l 1 2,4,6 -trichlorophenol Grab EPA625 <10.00 ug/l 1 Base -neutral compounds Acenaphthene Grab EPA625 <10,00 ug/l 1 Acenaphthylene Grab EPA625 <10 00 ug/l 1 Anthracene Grab EPA625 <10.00 ug/I 1 Benzidine Grab EPA625 <100.00 ug/l 1 Benzo(a)anthracene Grab EPA625 <10.00 ug/l 1 Benzo(a)pyrene Grab EPA625 <10.00 ug/l 1 3,4 benzofluoranthene Grab EPA625 <10.00 ug/I 1 Benzo(ghi)perylene Grab EPA625 <10.00 ug/1 1 Benzo(k)fluoranthene Grab EPA625 <10.00 ug/l 1 Bis (2-chloroethoxy) methane Grab EPA625 <10.00 ug/1 1 Bis (2-chlorcethyl) ether Grab EPA625 <10.00 ug/l 1 Bis (2-chloroisopropyl) ether Grab EPA625 <10.00 ug/l 1 Bis (2-ethylhexyl) phthalate Grab EPA625 <20.00 ug/l 1 4-bromophenyt phenyl ether Grab EPA625 <10.00 ug/l 1 Butyl benzyl phthalate Grab EPA625 <10.00 ug/I 1 2-chloronaphthalene Grab EPA625 <10.00 ug/1 1 Form - DMR- PPA -1 Page 2 Annual Monitoring and Poliutant Sean Permit N6. 0020389 Monthv t� Outfall 001 Year 201 4-chlorophenyl phenyl ether Grab IEPA625 I <10.00 ug/1 1 Parameter Sample Type AnalyticalQuantitation Method Level Sample Result Units of Measurement Number of samples Base -neutral compounds (coat.) Chrysene Grab EPA625 <10.00 ug/1 1 Di -n -butyl phthalate Grab EPA625 <10.00 ug/l 1 Di-n-octyl phthalate Grab EPA625 <10.00 ug/l 1 Dibenzo(a,h)anthracene Grab EPA625 <10.00 ug/l i 1,2 -dichlorobenzene Grab EPA625 <10.00 ug/i 1 1,3-dicblorobenzene Grab EPA625 <10.00 ug/l 1 1,4 -dichlorobenzene Grab EPA625 <10.00 ug/l 1 3,3-dichlombenzidine Grab EPA625 <10.00 ug/l 1 Diethyl phthalate Grab EPA625 < 10.00 ug/l 1 Dimethyl phthalate Grab EPA625 <10.00 ug/l 1 2,4-dinitrotoiuene Grab EPA625 <10.00 ug/1 1 2,6-dimtrotoiuene Grab EPA625 <10.00 ug/1 1 1,2-diphenylhydrazine Grab IEPA625 <10.00 ug/1 1 Fluoranthene Grab EPA625 <10.00 ug/l 1 Fluorene Grab EPA625 <10.00 ug/l 1 Hexachlorobenzene Grab EPA625 <10.00 ug/1 1 Hexachlorobutadiene Grab EPA625 <10.00 ug/l 1 Hexachlorocyclo-pentadiene Grab EPA625 <10.00 ug/l 1 Hexachloroethane Grab EPA625 <10.00 ug/l 1 Indeno(1,2,3-cd)pyrene Grab EPA625 <10.00 ug/i 1 Isophorone Grab __ FPA62� , _ - _ __ _ _ _ .. <10.00 --ug/�,..r�- Naphthalene Grab EPA625 <10.00 ug/1 1 Nitrobenzene Grab EPA625 <10.00 ug/1 1 N-nitrosodi-n-propylamine Grab EPA625 <10.00 ug/1 1 N-nitrosodimethylamine Grab EPA625 <10.00 ug/l 1 N-nitrosodiphenylamine Grab EPA625 <10.00 ug/1 1 Phenanthrene Grab EPA625 <10.00 ug/1 1 Pyrene Grab EPA625 <10.00 ug/1 1 1,2,4,-trichlorobenzene Grab EPA625 <10.00 ug/l 1 I certify under penalty of law that this document and all attachments were prepared under my direction and supervision in accordance with a system to design to assure that qualified perdonnel properly gather and evaluat the information submitted. Based on my inquiry of the person or persons that manage the system, or those persons directly responsibel for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate and complete. 1 am aware that there are significant penalties for submitting false information, including the possibility of fines and imprisonment for knowing violations. Matt Zapp Authorized Representative name Signatur Date Form - DMR- PPA -1 Page 3 Toxicity Testing FACILITY NAME AND PERMIT NUMBER. PERMIT ACTION REQUESTED: RIVER BASIN - Benson WWTP, NCO020389 Renewal Neuse Part E 4 Toxicity Testing Data Summary of Submitted Biomomtormg Test Information Organism. Ceraodaphnia Dubia Date Pipe Collection Testing Submitted Number Date Date Method Summary 12/07/16 001 11/28/16 11/30/16 Chronic Pass/Fall PASS 02/02/17 001 01/23/17 01/25/17 Chronic Pass/Fail PASS 05/04/17 001 04/24/17 04/26/17 Chronic Pass/Fail PASS 07/27/17 001 07/17/17 07/19/17 Chronic Pass/Fall PASS Organism. Pimephales Promelas Date Test Start End Submitted Vessel (ml) Date Date Method Summary 12/08/16 400 11/29/16 12/06/16 Chronic Fathead ChV >100 Minnow Multi - Concentration Test 02/02/17 400 01/24/17 01/31/17 Chronic Fathead ChV >100 Minnow Multi - Concentration Test 05/04/17 400 04/25/17 05/02/17 Chronic Fathead ChV >100 Minnow Multi - Concentration Test 07/28/17 400 07/18/17 07/25/17 Chronic Fathead ChV >100 Minnow Multi - Concentration Test Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 07/27/17 Facility: TOWN OF BENSON WWTP NPDES#: NCO020389 Pipe#: 001 County: JOHNSTON Laboratory] rr ng Te t: RITECH LABS, INC. �/ Comments : Signature of L ora ory Supervisor I * PASSED: 0.75% Reduction * Work Order: Environmental Sciences Branch MAIL ORIGINAL TO: Div. of Water Quality N.C. DENR 1621 Mail Service Center Raleigh, North Carolina 27699-1621 Chronic Pass/Fail Reproduction Toxicity Test :ONTROL ORGANISMS1 2 3 4 5 6 7 8 9 10 11 12 # Young Produced 24 22 22 24 22 19 24 23 13 24 2424 Adult (L) ive (D) ead L L IL IL IL IL IL IL IL IL IL IL sffluent 9011. 'REATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Control CV 14.636% # Young Produced 22 27 23 24 23 15 23 13 23 23 25 22 % control orgs producing 3rd brood Adult (L) ive (D) ead L L L L L L L L L L L L 11 100 Chronic Test Results Calculated t = Tabular t = t Reduction = 0.75 Mortality Avg.Reprod. 0.00 22.08 Control Control 0.00 21.92 Treatment 2 Treatment 2 PASS FAIL X Check One 1st sample 1st sample 2nd sample Complete This For Either Test PH Test Start Date: 07/19/17 Control 7.94 8.07 8.18 8.03 8.01 8.06 Collection (Start) Date Sample 1: 07/17/17 Sample 2: 07/19/17 Treatment 2 7.49 7.82 8.06 7.87 7.63 7.90 Sample Type/Duration 2nd 1st PIF s s s Grab Comp. Duration D t e t e t e I S S a n a n a n Sample 1 X 24.0 hrs L A A r d r d r d U M M t t t Sample 2 X 24.0 hrs T P P 1st sample 1st sample 2nd sample D.O. Hardness (mg/1) 44 . ::........ Control 7.98 7.81 8.22 7.48 8.10 7.24 Treatment 2 7.81 7.76 8.27 7.39 7.98 7.11 Spec. Cond.(gmhos) 155 215 374 .......... Chlorine (mg/1) ,,,,,.,, <0.1 <0.1 LC50/Acute Toxicity Test Sample temp. at receipt(OC) .,....,. 1.0 0.6 (Mortality expressed as %, combining replicates) Note: Please Concentration Complete This Section Also Mortality start/end start/end Control High Con^ LC50 = % Method of Determination 95% Confidence-rLimits Moving Average Probit -- % Spearman Karber _ Other Organism Tested: Ceriodaphnia dubia Duration(hrs): Copied from DWQ form AT -1 (3/87) rev. 11/95 (DUBIA ver. 4.41) AH D. 0. Meritech, Inc, / Mini Chronic Pass/Fail Test: Cenodaphnia dubie Incubator # Client: ` f�.Y eorl Pipe*: 001 County:���-�+j n date Start: 7-0-(7 Date End: -7 NPDES #: NC i Date /Time of Culture Transfer: -?-f . I7/ 1QjqAM Time Start: 12: 994zn Time End: Dilution Water: Lake Brandt Date/ Time Neonates born: ; or 1st Renewal Date: :Z-21-17 Time: _ I I Test Organism Source: Tray # Age ofeonates at Test Start- �2. �� hours 2nd Renewal Date. - Time: Stirred / Aerated for D.O : Y /9 Randomized:UY / N Culture Tray Temp- q�,°C Analyst(s): INR, cD, LV, ss Reviewed by: � Dav 92 1 2 3 4 5 6 7 8 A in 11 17 #Young Produced 1 0 1 0 1 0 1 0 1 0 1 0 1 0 1 0 0 0 0 0 Adults Live / Dead I L I t-- I L I L I (- I L, I L. L- L I L- L I L, Dav 05 1 7 3 4 5 8 7 R A 1 n 11 17 # Youn Produced I " K 8 9 10 11 12 % • III Ifol �( 1 0 S 0 0 Adults Live/ Dead L- L % L L L- � L L ✓.- L, /✓ pay #7 1 2 3 4 5 6 7 8 9 10 11 12 #Young Produced III Ifol q 1 0 0 0 0 Adults Live I Dead L, C L Adults Live / Dead- I L-, IL (, I L. L L L, L. fgj EQ t l Total Produced 12N21 2. Percent of Control producing third brood: % V77 - Test Sample Organism Reproduction Effluent %: - Dav #2 1 2 3 4 5 6 7 8 9 10 11 17 # Young Produced 0 0 0 1 0 1 0 1 0 0 1 0 1 0 0 0 0 Adults Live I Dead L, L L I L I L-, IL L I L. LL L L, L. Dav #5 1 2 3 4 5 6 7 8 9 1 n 11 17 # Young Produced 1 1211 Itf1% 11:11 WE 151-71/1017 0 hours Sam le 2 ! 1 Hardness 1 1;� Adults Live I Dead l__ I Z, Z- Z- Spec. Gond. (umhos/cm) � � � L. L I iL L. Dav 97 1 2 3 4 5 8 7 R 4 1 n 11 1 19 #Young Produced j G / C9 11:11 al ,:71:3 0 hours Sam le 2 ! 1 Hardness 1 1;� Adults Live/ Dead L L Z- Z- Spec. Gond. (umhos/cm) � � � L. z� L. .Total Produced Comments: Sample 1: -7-17-1 7 Sample 2: t ei- l Sample Information Batch # 100% pH G / C9 Duration Sam le 1 ,:71:3 0 hours Sam le 2 ! 1 Hardness hours Batch # �"l� �� Sample1 Sample2 'transfer Day 0 2 5 arf�F�i' {'gym Hardness 'TV( lRr (m 81L ) FikrF;�tr N Spec. Gond. (umhos/cm) � � � 104 z� Chlorine fgj EQ t l ReTetpPSiample e i 1 I, �5 Ih�'�^��txv f� t>I � VIM �r £I e pH 1stSamDle Control'v"'f Sample 71 b Inman nnal D.O. 1st SamDle Control Sample 71 b u niton near Tamm. 1 st Sample Transferred by Fed by. Day o/ L - Day 1 A Day 2 Day 3 U/ Day 4 L��r Day 5 Day 6 Terminated by: 2nd Sample 2nd Sample BIIK Sig g,&I 7r$;7 7,�3 �yU n t a final —TT073— final 2nd Sample 2nd Sample si�'l 7r 57 P1 ill Initis final mllla final 2nd Sample 2nd Sample aq,� ;ats+ l'71 r 3 1, V nrt a final mr P firml Control Sample Transferred by Fed by. Day o/ L - Day 1 A Day 2 Day 3 U/ Day 4 L��r Day 5 Day 6 Terminated by: 2nd Sample 2nd Sample BIIK Sig g,&I 7r$;7 7,�3 �yU n t a final —TT073— final 2nd Sample 2nd Sample si�'l 7r 57 P1 ill Initis final mllla final 2nd Sample 2nd Sample aq,� ;ats+ l'71 r 3 1, V nrt a final mr P firml MERITECH, INC. MeritechSample ID#: o'S17I5 n Bloassay Sample Chain of Custody 642 Tamco Rd, Reidsville NC 27320 Phone: 1-336-342-4748 Fax: 1-336-342-1522 Toxicity Supervisor email: mike.reed@meritech4abs.com Web Site: www.moritech4abs.com Client: Contact Person: Address: City: County. PO#: &- NPDES#: NCU' 010 Phone(_%� ) _ Pipe #: CACI � Zip: 2 -i 5 l a ` SAMPLE INFORMATION Sample Site: t Sample Type: ❑ Grab Q Composite # of containers: Sampling Time: Start Date, Start Time: OS AM PM End Date: 7� [ ('� End Time: 4 PM Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory' Collector's Name: Print: -� f'e-�; C Z-- i\ � Signature: TOXICITY TEST INFORMATION Test Required: ❑ Chronic (7 days) ❑ Acute (24-48 hours) IWC: -'"7o % Comments: Test Organism: Fjj Ceriodaphnia dubia (water flea) ❑* Pimephales promelas (fathead minnow) ❑ Mysldopsls bahia (shrimp) Test Concentrations (if multiple dilutions): � y /� SHIPPING INFORMATION Relinquished by:6 2. a -f Date. 2s• Time, PM Received by: ' '6 Date. �•�% Time: 95PM Relinquished bw'z o/ �,.ky Date: _7"2 '% 7 Time: CSS AM Received by: VC/Date: Time. AM PM Relinquished by: Date., Time- AM PM Received by: Date: Time. AM PM Relinquished by: Date: Time. AM PM Received by: Date: Time, AM PM Sample Temperature CC): Method of Shipment ❑ UPS ❑ Fed EX ❑ Meritech Pick-up 0 Delivered ❑ Other Samplet shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED •` SAMPLE RECEIVING (Laboratory Use Only) Relinquished by. l� �� nAe S Received by: / "i( ►^ Date: —7-)H-7 Time: &: AM PM y l� Sample Temperatures CC): t • (% I I 1 Sample Condition: WHITE = Laboratory copy YELLOW = Client copy MERITECH, INC. Mentech Sample ID fk (-5q zG 1-1LE K Bioassay Sample Chain of Custody •Jr 642 Tamco Rd, Reidsville NC 27320 Phone: 1-336-342-4748 Fa)c 1-336-342-1522 Toxicity Supervisor email: mike.reed(Mmeritech-labs.com Web Site: www.maritech-labs.com CLIENT INFORMATION Client: ,� ii;1 �u� YJ &k 7G>�SU W PO#: Contact Person: pt oL - tAxIT) NPDES#: NC cxr1!-07 SQN Address:—T\8 , e-kv-n-Je .1m (—,r-c—.Y Phont -19) a q.z Zl-) City: Pipe M C.L3 •1 County State: ZIP: ?115 ZA SAMPLE INFORMATION Sample Site: E m u revel C Sample Type: ❑ Grab ®'Composite # of containers. Z - Sampling Time: StartDate: `7, k j"kl'L Start Time: p D ✓ PM End Date: -1 In, End Time: PM `** Triple rinse sample container with sample before rifling. Completely rill the sample container with no air space. Pack the sample cooler completely in Ice. The sample must be < 6.0°C upon receipt at the e� laboratory' Collector's Name: Pnnt: �!ea e RjaZ—� Signature t"1 1(� TOXICITY TEST INFORMATION Test Required: 0 Chronic (7 days) Test Organism: © Ceriodaphnfa dubia (water flea) ❑ Acute (24-48 hours) fi] Pimephales prome/as (fathead minnow) ❑ Mysidopsis bahla (shrimp) IWC: 4)3 % Test Concentrations (if multiple dilutions): Comments- SHIPPING INFORMATION Relinquished by: C.L, Date: 1 I� l�~` Time: 9i �j� EAM PM Received by: .tip ✓�'�� Date, 2 - t�� • Time: '' j ct w PM Relinquished by n ,- �;y� r� r �-z 2_ � Date: `%'..2 4y / '� Time: AM Received by: Date: Time: AM PM Relinquished by: Date: Time: AM PM Received by: Date: Time: AM PM Relinquished by: Date: Time: - AM PM Received by: Date: Time: AM PM Sample Temperature (°C): Method of Shipment ❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑Delivered ❑ Other " Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED " SAMPLE RECEIVING (Laboratory Use Only) r _ Relinquished by Received 4y, Date: _`�� —� l Time: AM Sample Temperatures CC): � � Ck-1 tI I Sample Condition: WHITE = Laboratory copy YELLOW = Client copy Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 07/27/17 Facility: TOWN OF BENSON WWTP NPDES#: NCO020389 Pipe#: 001 County:JOHNSTON Laboratory Performing Test: MERITECH LABS, INC. 1.0833 Reduction: 0.751 CONTROL 901 Effluent # Replicates 12 12 Female Live 12 12 Adult Male 0 0 Adult Dead 0 0 Adult Mortality 0.001 0.00% # Neonates 265 263 Mean # Neonates 22.083 21.917 Standard Deviation 3.232 3.965 Coefficient of Variation 14.6361 C Fisher's Exact Test A = 12 B = 12 a = 12 b = 12 a/A = 1.00 b/B = 1.00 Success is: survival Critical b value = 8 12 > 8 The test concludes that the proportion of survival is not significantly different for the control and the effluent groups. Test Passes! SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA ORDERED OBSERVATIONS i Group Neonates Centered i Group Neonates Centered 1 C 13 -9.0833 13 E 23 1.0833 2 E 13 -8.9167 14 E 23 1.0833 3 E 15 -6.9167 15 E 23 1.0833 4 C 19 -3.0833 16 C 24 1.9167 5 C 22 -0.0833 17 C 24 1.9167 6 C 22 -0.0833 18 C 24 1.9167 7 C 22 -0.0833 19 C 24 1.9167 8 E 22 0.0833 20 C 24 1.9167 9 E 22 0.0833 21 C 24 1.9167 10 C 23 0.9167 22 E 24 2.0833 11 E 23 1.0833 23 E 25 3.0833 12 E 23 1.0833 24 E 27 5.0833 3 SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.) COEFFICIENTS AND DIFFERENCES i x(n-i-1) x(i) a(i) x(n-i-1) - x(i) 1 5.0833 -9.0833 0.4493 14.1666 2 3.0833 -8.9167 0.3098 12.0000 3 2.0833 -6.9167 0.2554 9.0000 4 1.9167 -3.0833 0.2145 5.0000 5 1.9167 -0.0833 0.1807 2.0000 6 1.9167 -0.0833 0.1512 2.0000 7 1.9167 -0.0833 0.1245 2.0000 8 1.9167 0.0833 0.0997 1.8334 9 1.9167 0.0833 0.0764 1.8334 10 1.0833 0.9167 0.0539 0.1666 11 1.0833 1.0833 0.0321 0.0000 12 1.0833 1.0833 0.0107 0.0000 I W = X 216.0428 287.8333 Calculated W = 0.751 Critical W 0.884 0.751 < 0.884 The reproduction data is not normally distributed evaluated at a 99v confidence interval Test Failed! Wilcoxon Rank Sum Test Rank Sum - Effluent Group(treatment): 147 Control'Group: 153 Mann-Whitney U = 69.0 n1(treatment) = 12 n2(control) = 12 The critical value from TABLE A.15 (0.01 Level of T) for the above n values: Critical T = 109 147 > 109 The Sum of the Ranks of the effluent group is greater than the significant I value from the table. Therefore there is no significant difference between the means of the effluent and control groups and the test passes. TEST PASSES Chronic Test PASSES The reduction was less then 20a Effluent Toxicity Report Form -Chronic Fathead Minnow Mufti -Concentration Test Date:7/28/2017 Facility: Town of Benson NPDES # NC00 20389 MAIL ORIGINAL TO: Water Sciences Section Aquatic Toxicology Branch Division of Water Resources 1621 Mail Service Center Raleigh, N.C. 27699-1621 Test Initiation Date/Time 7/18/2017 7:00 PM Pipe #• 001 County: Johnston Avg Wt/Surv. Control 0 906 % Eff. Repi. 1 2 3 4 Control Surviving #ffoi 0 10 10 10 Original #0 41 10 10 10Wf/original (mg)905 386 1 0.841 0.878 1.000 22.5 Surviving # 9 10 10 10 Original # 10 10 10 10 Wt/onginal (mg) 0.939 0.751 0.904 0.834 45 Surviving # 10 10 10 10 Original # 10 10 10 10 Wt/onginai (mg) 0.919 0.806 0.926 0.864 75 Surviving # 10 10 10 10 Original # 10 10 10 10 Wt/original (mg) 0.946 0 938 0.804 0.899 90 Surviving # 10 9 10 10 Original # 10 10 10 10 Wt/original (mg) 0.858 0.862 0.849 0.978 100 Surviving # 10 10 10 10 Original # 10 10 10 10 Wit/original (mg) 0.822 0 881 0.880 1 0 856 Water Quality Data Control pH (SU) [nit/Fin DO (mglL) Ind/Fin Temp (C) init/Fin High Concentration pH (SU) InitlFin DO (mg/L) InitlFln Temp (C) Init/Fin Sample Collection Start Date Grab Composite (Duration) Hardness (mg/L) Alkalinity (mg/L) Conductivity (umhos/cm) Chlorine(mg/L) Temp. at Receipt (`C) % Survival 100.0 Avg Wt (mg) 0.906 % Survival 97 5 Avg Wt (mg) 0.857 % Survival 100.0 Avg Wt (mg) F70.879 % Survival 100.0 Avg Wt (mg) 0.897 % Survival 97.5 Avg Wt (mg)F__0 -887 % Survival 100 0 Avg Wt (mg)F--0 860 Day 0 1 2 3 4 6 6 8.14 / 7.94 8.21 / 7.92 8.17 1 7.94 819 1 8.04 8.22 1 7.90 8.22 / 7.85 8.15 1 7.84 8 23 1 7 00 7.88 / 7 00 8.00 1 7.02 7.78 1 7 76 8.09 / 7 00 8.08 / 7.13 7 97 16.42 24.3 1 24.1 24 3 / 24.5 24.7 / 25 4 24.1 1 25.9 24.1 / 24.2 24.4 / 24.9 24.7 / 25.8 0 1 2 3 4 5 6 6.98 1 7 43 7 29 / 7.42 7.32 1 7 61 7.39 / 7 84 7.72 / 7.58 7.32 / 7.56 7.29 / 7 56 7.64 / 6 88 7.44 / 6.58 7 96 / 6.85 7.82 / 7.69 7.84 / 6 37 7.50 1 7 02 7.99 / 6.65 24.6 / 24 2 1 24.0 1 24 3 1 24.5 1 25.1 24.1 / 25.9 24.8 / 24.8 25.1 1 25 0 24.8 1 24.3 1 2 3 7/17/2017 7/19/2017 7120/2017 Normal V P'( ChV 7100 240 24.0 24.0 24 40 41 21 38 39 223 1 386 1 394 <0.1 <0.1 <0.1 1.0 0.6 17 Dilution H2O Batch # 1179 1180 1181 Hardness (mg/L) 44 44 42 Alkalinity (mg/L) 52 53 53 Conductivity (umhos/cm) 205 207 197 Test Organisms i' Cultured In -House )w Outside Supplier Hatch Date: 7117117 Hatch Time: 3:00 pm CT Stats Survival Growth Overall Result Normal V P'( ChV 7100 Ham. Var. ri (Ivy NOEC 100 100 LOEC >100 >100 ChV >100 >100 Method Steel's DunnetVs Stats Survival Growth Conc. Critical Calculated Critical Calculated 225 10 16 2.41 1.1102 45 10 18 2.41 0.6174 75 10 18 2.41 0.2096 90 10 16 2.41 04361 100 10 18 2.41 10479 Meritech, Inc. Chronic Fathead Minnow Benchsheet Transfer and Feeding Dates and Times Client: Benson # of Organisms per Chamber. 10 NPDES #: 0020389 Test Vessel Size: 400 ml Initiated by: ^� Test Solution Volume: 260 ml Date/Time Fed: 7/18/17 , W. •Q r Temp. of Stock: 74.3 °C Date/Time Born: 7/17/17 3:oo PM Cr # of Reps: 4 Organism Source: Aquatox, Inc. Test Organism: Pimephales aromelas Start Date: 7/18/17 Time: 7-,6,0 P1r+ End Date: 7/25/17 Time:? :3 6prI Date Time Initials Randomization: Yes No Incubator #: Time 2 Initials Day 1 Analyst(s): MR, CD, LV, SB Test Termination Data: Initials/Signature: WAA�j,1�n.�,r��—�� End Date: 7/25/17 / /17 Transferred by: Fed by: Date Time Initials Date Time 1 Initials Time 2 Initials Day 1 7/19 -�--� Lv'%L� Day 0 7/ 18 `� =� Lti 7 Day 2 7/20 7:4-nTQ Day 1 7/19 �- % ,� [.✓ 3 ,tJ Day 3 7/21 Gyp �J3 Day 2 7/20 ,� LA./ %- M� Day 4 7/22 7 � Day 3 7/21 . C)04rm Lv� `2 LC?P17 &AI/ Day 5 7/23 : i.SP �n� Day 4 7/22 �, ',}f �J✓ (� 5 n� C.0 Day 6 7/24d ay �/_ Day 5 7/23 k hn V\, Day 7/24 Test Termination Data: Initials/Signature: WAA�j,1�n.�,r��—�� End Date: 7/25/17 / /17 Client: Benson MER/TECH,. INC_ ENVIROA NMENTAL LBORATORIES A Division ivisioof Water Technology and Controls /nc Chemical and Physical Determinations NPDES#: 0020389 Start Date: 7/18/17 End Date: 7/25/17 Dav Concentration: control 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks Soft Synthetic Fesh►vate Batch#: 7 %. �; —7• 1 � O `7 ('31 7 � pH: Initial .{Lk 4q1, 11 11A .0 71-7 D.O.: Initial k ucf D.O.: Initial Final 7 r e'I qr Final 77t g 17,K g'� 7. G9 D.O.: Initial 9:93 212 t 60 -7 ,;ice , g ` Final �' ( � � ` � `7, 0 t :2q. ; a , Temp.: Initial `1'7<�3 7 , -7 �� + ? t}. ° ► l-{ - Final 'oZ '1 +S�7. 3 10 a 3S Residual Chlorine: Conductivity: Initial NY -Z., in / 7 1 C11- 9f Final U I Residual Chlorine: Svc ( Lrz Lw Lo f v L�1 �'1 Ga = Hardness: Ll Alkalinity: S' 7 z o Dav Concentration: 22.50% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH: Initial �/, lJ %. �; —7• 1 � O `7 ('31 7 � Final 4q1, 11 11A .0 71-7 D.O.: Initial k ucf D.O.: Initial 7 r r U P,1) Final ti -a-7, Sip1 g'� 7. G9 Final 9 t J cC + 1-5 -7 ,;ice , g ` Temp.• Initial 2- ',1, Final � , � ` � � :2q. ; a , Final '� 2i `� r� �� ?moi• 5 a fl `),'0 Conductivity: Initial O Ql 5 3 10 a 3S oZ"L7 Residual Chlorine: Final in I-0-1$ � Residual Chlorine: Dav Concentration: 45.0% 0-1. 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH: Initial 7,b,3 —7• 1 YS - Final �.ii' %t ? ;j G D.O.: Initial k ucf f, 7 r r U Final to &k 7. G9 G+ 40 707 `I 9 t J Temp.. Initial 714 - `I g ` Final � , � ` � � :2q. ; a , Conductivity: Initial 110,11 1 7 `� � � 5 a fl `),'0 Final a 5 3 10 t Residual Chlorine: Gd z 1 in I-0-1$ � Client: Benson MER/TEC'H� 6W"ROW MENTAL L-"" [3 Rt ES A (J!v!s/fan P/ Lifa lam 1bCllnele 9Y AIl4'Y Cenfrofs. /no Chemical and Physical Determinations NPDES#: 0020389 Start Date: 7/18/17 End Date: 7/25/17 Dav Page 2 of 2 Concentration: 75% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH: Initial. '3� ,. ` ,�- '7,L17 -7, , `(1 7 3 Final Final L -7'Q 71 g ?1 L 3 7h 5- � D.O.: Initial % D.O.: Initial ` 7 1 7A la7� 7, 4 2 7f Final �L Final �Q �}' 6f 3 1 T G Ef ! t6YTemp.: Temp.: Initial7 .� a��-,� Initial,1 �j pZ`i'� ;ZI,69 Final Final �- �� t I. ��'��r`1• �5 G� 7 Conductivity: Initial Conductivity: Initial 3-/0 x(00 j70 -3 - '� -7jFinal Final 5—)-q L S9 1 1,1,?3-70 !;,& S 3 Residual Chlorine: Residual Chlorine: Z 4' 1 0� i �� t Lo-, � i- w 1 'e- Day Concentration: 90% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH: Initial ( ,. ` ,�- -;? `!- -7, , `(1 7 3 7 A y Final 5 L 71 -7 S 7. IS 7h 5- � D.O.: Initial % /-) W'H 7,7 7f sf 1 t • o 7 54> Final �L ,�J'4% ,lj 6f 3 -It o 51? 7, - Temp.: Initial7 .� a��-,� �` {. �` �j pZ`i'� ;ZI,69 Final .,j �"1 ��_� �`1'� Ll .a— 0 ,�, . Conductivity: Initial a 13 3-/0 x(00 j70 -3 - '� -7jFinal Q �i 5—)-q L S9 1 Residual Chlorine: 40 1 J,, ,V) bm t Lcn fin• G� Day Concentration: 100% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH: Initial -;? `!- -7 -7.-)- 7, ?) 7 A y Final L (4; % Vq 1 C K 7h 5- � D.O.: Initial 764 %, b i� c / 7 54> 7 Final 1 7 7, - Tem Initial `3 pZ`i'� a,'r Final ��_� �`1'� aJ� 5-`�/ 0 ,�, . Conductivity: Initial Final 5—)-q L S9 1 Residual Chlorine: c 0 : f Hardness: 21A Ho L 1 Alkalinity: MERITECH, INC. Mortality Data: Chronic Fathead Test Client: Benson NPDES#: 0020389 Start Date: 7/18/17 End Date: 7/25/17 Concentration Control Tuesday Wednesday Thursday 0 Friday Saturday Sunday Monday Tuesday Rep A 0 Friday Saturday Sunday Monday Tuesday Rep A 0 Rep B 0 ��L 0— , Rep B 0 � V Rep C 0_ I Rep C 0_ Rep D 0 Rep D 0 Concentration 22.50% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 v Rep B 0 � V Rep C 0_ Rep D 0 Concentration 45.0% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 n ► Rep B 0 Rep C 0� v d Rep D 0 MERITECH, INC. Mortality Data: Chronic Fathead Test Client: Benson NPDES#: 0020389 Start Date: 7/18/17 End Date: 7125/17 Concentration 75% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 �; 0 Rep B 0 _ V Rep C 0 Rep D 0 Concentration 90% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday SIA Rep A 0 o Rep B 0 D � Rep C 0 C Rep D 0_ Concentration 100% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 0 V Rep B 0 _0 o 0 Rep C 0 �} t 0 RepD 0 �2 ��c Facility: Benson NPDES #: 0020389 ME ®T "V ENVIRONMENTAL LABORATORIES A Ofvfsion of Water Technology and Controls, Inc FISH LARVAL SURVIVALIGROWTH DATA SHEET CHRONIC FATHEAD MINNOW Date of Test: 7/18/17 Initial weights taken on: 6',27-17 by: 1—\/' Final weights taken on: 7- 2:L- by: LL.,/ Page: 1 of 2 Outfall: Organism: Pimephales promelas CONC REP PAN WT. (mg) PAN + ORG. WT. (mg) WT. OF ORG. (mg) # ORG. MEAN WT.IORG. (mg) SURV % L �+ 0 A �5 3.5�'�. �� �� /D �t qr 100 B -3a 939, 7 (l. � 10 D. 00 035,16 F1-6 7k 10 o' D EL, 98 Ez/C/,W /C)' 6 6 10 1l • OOD ld 0 22.50% A r T' b B �. "l,Oa 7 �1 10 0T,7.51 Ida D N0,73 9L-161-,-7 q1. C /0 10, q0q l D W1, 3q gs x73 �• q /0 lo --339 166 45.0% A I a7 qt Iq 10 q F B f 9,53J7 W, D 6 16 0.306 100 D 939,5S RY7,91 q, Q6 10 01gq6 lool D R�3. rS I �?5I; 7 10 1 0'?6q l�c� Facility: Benson NPDES #: 0020389 Date of Test: 7118117 WHRI IL=,C ,® I ENVIRONMENTAL LABORATORIES A Division of Water Yechnofogy And Controls, 1nr- FISH LARVAL SURVIVALIGROWTH DATA SHEET CHRONIC FATHEAD MINNOW Initial weights taken on: 6.,`a7- by: L✓ Final weights taken on: � by: Lk/) - Page: 2 of 2 Outfall: Organism: Pimephales�promelas CONC REP PAN WT. (mg) PAN + ORG. WT. (mg) WT. OF ORG. (mg) # ORG. MEAN IWT.IORG.(mg) SURV % 75% A . 5 �(. tD 0. �l ,� ov C� n C �Jv l J: V v • �1 f (" r { �✓ f /00 90% A v3 > 1�r� B 03`(1 Z r J F(9- �2 C 035, q'7 Yq-"-)'. t W, q2 q, 7F -4117 L loo 100% A B Wtl 7. v c 0-1 D g3�/ (� L r 15L 1 v ().E56 �a , MERITECH, INC. MentechSample ID#. U��111 � Bioassay Sample Chain of Custody •� 642 Tamco Rd, Reidsville NC 27320 Phone: 1-336-342-4748 Fax: 1-336-342-1522 Toxicity Supervisor email: mike.reedAmeritech-labs.com Web Site: www.moritech4abs.com TOXICITY TEST INFORMATION Test Required: © Chronic (7 days) Test Organism: ❑X- Cerlodaphnia dubla (water flea) ❑ Acute (24-48 hours) Eq Pimephales prome/as (fathead minnow) C1 F1Mysidopsis Bahia (shrimp) IWC: `0 % Test Concentrations (If multiple dilutions): Comments: SHIPPING INFORMATION CLIENT INFORMATION Client: �2r C � \-r e- Relinquished by: Date: ~%�2.j 1 7 PO#: Contact Person: Qcx\t L Th I\ C. 1r- NPDES#: NCoO 2.-C, 31e1 Address: "1-I 0 VN (:r,r- Phoney i 1' «94 (13-13 City: fvL 1 G s,,, cl Pipe #• CAD C County } ,11L,1r 4'N State: Zip: S 7 - t -k SAMPLE INFORMATION Sample Site: '��y L1jJ I Sample Type: ❑ Grab Composite # of containers, Z. Sampling Time: ))� ��© Start Date: %,4 �t� Start Time: PM End Date: -11-a l r -L End Time: `.`�: JCS AM ` PM Triple rinse sample container with sample before filling. Completely rill the sample container with no air space. Pack the sample cooler completely in ice. The sample must be < 6.0*C upon receipt at the laboratory"* QJ—, Collector's Name: Print: `) v -� C ,c"�� . Signature: A- _P TOXICITY TEST INFORMATION Test Required: © Chronic (7 days) Test Organism: ❑X- Cerlodaphnia dubla (water flea) ❑ Acute (24-48 hours) Eq Pimephales prome/as (fathead minnow) C1 F1Mysidopsis Bahia (shrimp) IWC: `0 % Test Concentrations (If multiple dilutions): Comments: SHIPPING INFORMATION Relinquished by:_. __ r(%, LL'uti-�- Date: % Z% Received by: tAAI Date: 712 (/ l % Time: Relinquished by: Date: ~%�2.j 1 7 Time: Received by: Relinquished by: Received by: Relinquished by: Received by: Date: Date: Date: Date: Date: Time- -rime: Time: Time: Time: AM PM AM PM AM l -P' -M) AM PM AM PM AM PM AM PM AM PM Sample Temperature (°C): Method of Shipment: ❑ UPS ❑ Fed IX ❑ Meritech Pick-up ❑ Delivered ❑ Other *' Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED ** -� SAMPLE RECEIVING (Laboratory Use Only) Relinquished by: Received by Date: Time. AM �M j e / / I- Sampfe Temperatures CC): 1 f t I ! Sample Condition: WHITE = Laboratory copy YELLOW = Client copy Title: Benson File: BensonGrowth Transform: NO TRANSFORMATION Shapiro - Wilk's Test for Normality ---------------------------------------------------------------------------- D = 0.0701 W = 0.9714 Critical W = 0.8840 (alpha = 0.01 , N = 24) W = 0.9160 (alpha = 0.05 , N = 24) Data PASS normality test (alpha = 0.01). Continue analysis. Title: Benson File: BensonGrowth Transform: NO TRANSFORMATION Bartlett's Test for Homogeneity of Variance ---------------------------------------------------------------------------- Calculated B1 statistic = 2.8267 (p -value = 0.7267) Data PASS B1 homogeneity test at 0.01 level. Continue analysis. Critical B = 15.0863 (alpha = 0.01, df = 5) = 11.0705 (alpha = 0.05, df = 5) I i Title: Benson File: BensonGrowth Transform: NO TRANSFORMATION ANOVA Table ---------------------------------------------------------------------------- SOURCE DF SS MS F ---------------------------------------------------------------------------- Between 5 0.0078 0.0016 0.3980 I Within (Error) 18 0.0701 0.0039 ---------------------------------------------------------------------------- Total 23 0.0779 ---------------------------------------------------------------------------- (p-value = 0.8437) Critical F = 4.2479 (alpha = 0.01, df = 5,18) 2.7729 (alpha = 0.05, df = 5,18) Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05) I � l OlWIAW NH I Q I I c I I ro I n N ct F -t F j otov&est0 0 0 Ln cn 0 F -J rA FP li Oh tP +? I m i • F-' F-' H H N 00000 Ot rn rn m ON tP tp A. A A h'F1YHI-A F1 F' F' Y FA vv�vv � • • 00000 t?FYONeP m tO lO .4 t4 N N N N O N H W Y- F -j ct (DY .. (D >r w CD 0 ccIr w m rt (D O ri 0 in m H p (A Fj n O 14 m I � y k�] N 0 F N W r'h O t'S O n 0 0 rr H O H t1S rt mm� O \V Ct H H z F-' O N ',s' N rr I I --t Yt 1 OOMMOH 1 0 0 0 0 0 0 O 00 00 O 00 1p Ll OD w-4 U7O w mOlOE) -1O1 00 ODODOOO 000000 aO oO O0 O aD tO Ln 00lOJ (nO OD 00000000 H 0 o 0 H O4Nwa% i FPwO HFA v01%t0JO t0WO11AN I ro (DN .. rn s� w rr Gl m rr (D O 0 0 H 0 (n F j cr � ct I I I o I � I I H I O n I I N t y e I W d t I H t I tit I I n 0 Fl O F1 n z O y I y I I H i H N (D pi O I I I"t y I O U] I t H I I o 4] H O z Cn Title: Benson File: BensonSury Transform: NO TRANSFORMATION Shapiro - Wilk's Test for Normality ---------------------------------------------------------------------------- D = 0.0150 W = 0.6138 Critical W = 0.8840 (alpha = 0.01 , N = 24) W = 0.9160 (alpha = 0.05 , N = 24) Data FAIL normality test (alpha = 0.01). Try another transformation. Warning - The first three homogeneity tests are sensitive to non -normality and should not be performed with this data as is. Title: Benson File: BensonSury Transform: NO TRANSFORMATION Hartley's Test for Homogeneity of Variance Bartlett's Test for Homogeneity of Variance ---------------------------------------------------------------------------- These two tests can not be performed because at least one group has zero variance. Data FAIL to meet homogeneity of variance assumption. Additional transformations are useless. Title: Benson File: BensonSury Transform: NO TRANSFORMATION ----------------------------------------------------------------------------- Steel's Many -One Rank Test - Ho: Control<Treatment MEAN IN RANK CRIT. SIG GROUP IDENTIFICATION ORIGINAL UNITS SUM VALUE DF 0.05 ------------------------- 1 Control ----------- 1.0000 ------- ------ ----- --- 2 22.50 0.9750 16.00 10.00 4.00 3 45 1.0000 18.00 10.00 4.00 4 75 1.0000 18.00 10.00 4.00 5 90 0.9750 16.00 10.00 4.00 6 100 1.0000 18.00 10.00 4.00 ---------------------------------------------------------------------------- Critical values are 1 tailed ( k = 5 ) P Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 05/04/17 Facility: TOWN OF BENSON WWTP Laborat Perfo Test x� �g��%.off X NPDES#: NC0020389 Pipe#: 001 County: JOHNSTON LABS, INC. Comments: e Signaureo story Supervisor I * PASSED: -2.83% Reduction * I Work Order: Environmental Sciences Branch MAIL ORIGINAL TO: Div. of Water Quality N.C. DENR 1621 Mail Service Center North Carolina Ceriodaphnia Raleigh, North Carolina 27699-1621 Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results Calculated t = -0.565 Tabular t = 2.508 CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Reduction = -2.83 # Young Produced 1121122125119117118119124123118122119J Mortality Avg.Reprod. Adult (L)ive (D)ead JAL IL IL IL IL IL IL IL IL IL IL IL Effluent a: 90t TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 li 12 # Young Produced 1125121124123121119121119121121116123 Adult (L)ive (D)ead JIL IL IL IL IL IL IL IL IL IL IL IL 0.00 Control 20.58 Control 0.00 21.17 Treatment 2 Treatment 2 Control CV 12.6790 PASS FAIL % control orgs producing 3rd100%brood pChec k One 1st sample 1st sample 2nd sample Complete This For Either Test pH Test Start Date: 04/26/17 Control 8.4 8.11 8.07 8.14 7.80 8.16 Collection (Start) Date Sample 1: 04/24/17 Sample 2: 04/26/17 Treatment 2 7.85 8.37 7.21 7.81 7.69 7.71 Sample Type/Duration 2nd s s s Grab Comp. Duration D 1st PIF t e t e t e I S S a n a n a n Sample 1 X 24.0 hrs L A A r d r d r d U M M t t t Sample 2 X 24.0 hrs T P P 1st sample 1st sample 2nd sample D.O. Hardness (mg/1) 47 ..... ..... . Control 7.52 7.26 7.85 7.13 7.50 7.29 Spec. Cond.(pmhos) 184 382 212 Treatment 2 7.58 7.25 8.18 7.16 7.42 7.28 Chlorine (mg/1)....... <0.1 <0.1 LC50/Acute Toxicity Test Sample temp. at receipt (°C) ........ 2.2 2.4 (Mortality expressed as combining replicates) Note: Please o o % Concentration Complete This Section Also % Mortality start/end start/end -71 LC50 = % Method of Determination Control 95% Coni i'Tdence Limits Moving Average Probit -- % Spearman Karber _ Other - High Conc. PH D.Q. Organism Tested: Ceriodaphnia dubia Duration(hrs): Copied from DWQ form AT -1 (3/87) rev. 11/95 (DUBIA ver. 4.41) Meritech, Inc. Mini Chronic Pass/Fail Test Ceriodaphnia dubia Incubator* Client. 2V-1-5 0'01 Pipe #: dal County: Date Start. , - j Date End: NPDES #, NC doh ZVcj Date /Time of Culture Transfer- Time Start: ; J& A Time End: Dilution Water. Lake Brandt Date / Time Neonates bom: , = /n- 1st Renewal Date: +-f - - 7 Z Time: Test Organism Source: Tra # Age of el t1. onates at Test Stal g y_ honors 2nd Renewal Date: -5 f -- j"7 Time. Stirred / Aerated for D.O.: Y / N Randomized..UY / N Culture Tray Temp: _2:q.7C Analyst(s): Me, Co, iv,ss Reviewed by: Control Oraaii3m cnnurua uctevn Collect! n (Startl Dates• Pay #2 1 2 3 4 5 6 7 8 9 10 11 12 #Young Produced 0 1 0 1 0 1 0 0 1 Q 1 0 0 0 0 0 Dead --- L-- L L --L L jo #Youno Produced 42 ua iFi 1 2 3 4 5 a 7 #Youn.q Proi 10 1 1 1 0 1 0 0 0 0 8 9 10 11 Adults Live / Dead 0 0 0 L Percent of Control producing third brood: &6 % Test Sample Organism Reproduction Effluent %: Dav ii9 1 7 A r # Young Produced 0 0 1 1 0 1 0 0 0 0 71 12— 0 0 0 0 0 0 Adults Live/ Dead L.-- (� t,_._ L.- n -..Are ' t �_�4r c,� iq 7 8j 9, 10, 11 12 Adults Live / Dead Day #7 1. 2 3 4 5 6 7 8 9 10 11 12 #Young Produced q Adults Live I Dead Total Produced Comments: Sample 1: Sample 2:� �1 Sample Information 100% PH G / C? Duration Sample 1 G hours Sample 2� a� O hours Transferred by. Fed by; Batch# 1 1 � Sample 1 Sample 2 *-j Day 0 l LV Transfer Day 0 2 5 Day 1 Hardness ! ` --t/ (11(1119/Ps�- Day 2 Spec. Cond.j Day 3 (umhaslcm) Day 4 Chlorine (mgfL} _ 1.s?r � �I 1 Day 5 ReceiptSampre Day 6 Temp, (°C) •;'j i ,a Terminated by-. PH 1st Sample 2nd S I -V. 2nd Sample nus rrrat mm m Rim D.O. 1st Sample 2 d S 1 Control Sample �f3� -V. 2nd Sample nus rrrat mm m Rim D.O. 1st Sample 2 d S 1 n Wye 2nd Sam le a ro- - ti 7 -t - imn Mai rnna -7 w muni f nal Temp. Ist5ample 2nd Sample 2nd Sample Coryirol A,y asr'� a`� �5�E) Sampley���!�n Control' Sample{ n Wye 2nd Sam le a ro- - ti 7 -t - imn Mai rnna -7 w muni f nal Temp. Ist5ample 2nd Sample 2nd Sample Coryirol A,y asr'� a`� �5�E) Sampley���!�n MERITECH, INC. Bioassay Sample Chaln ofCusfody 642 Tamco Rd, Reidsville NO 27320 Phone: 1-336-342-4748 Fax' 1-336-342-1522 Merilech sample to Toxicity Supervisor email: mike reed(almeritech-labs.com Web Site: www.meritech-labs.com Sampling Time: Start Date.4 CZE 1 `1 Start Time: C4 -"-'W q0 PM End Date: t}E A 1'Z End Time: , .,r _ a PM Triple rinse sample container with sample before filling. Completely till the sample container with no air space. Pack the sample cooler completely In ice- The sample must be < 6.0°C upon receipt at the laboratory"' Collector's Name: Print�j��u QStyp Ag r Signature - TOXICITY TEST INFORMATION Test Required: ® Chronic (7 (lays) ❑ Acute (24-48 hours) IWC. Comments: Test Organism: ❑ Ceriodaphnla dubla (water flea) W] Plnrephales prome/as (fathead minnow) ❑ Mysidopsis bahla (shrimp) Test Concentrations (if multiple dilutions) - SHIPPING INFORMATION Reitnquished Date: Time: l PM Received by. Date Time: (�AMS PM Relinquished by Date: f7 Time: 15-T7 7 AM Received by. Date: Time: AM PM Relinquished by: Date: Time: AM PM Received by: Date: Time: AM PM Relinquished by: Date: Time: AM PM Received by Date, Time: AM PM Sample Temperature (°C).- Method °C):Method of Shipment: ❑ UPS 0 Fed EX ❑ Meritech Pick-up ❑ Delivered [] Other Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED " SAMPLE RECEIVING (Laboratory Use Only) Relinquished by: Received by: Sample Temperal e Date: Time , 7 �" AM PN Sample Condition. WHiTE = Laboratory copy YELLOW = Client copy �� 1� r CLIENT INFORMATION Client 't PO#: Contact Person: ��,1\�� �i j s, �n1 _ NPDES#: NC vocd Q j Address:. °'IPJ Vey1J4:s a. Phone: J 2m City. U dv _SIC[ Pipe#. llc� County- `— �a �4o�J State: N 0-- Zip. ENC SAMPLE INFORMATION Sample Site: (C,t. �� Sample Type: ❑Grab Composite # of containers:`�� Sampling Time: Start Date.4 CZE 1 `1 Start Time: C4 -"-'W q0 PM End Date: t}E A 1'Z End Time: , .,r _ a PM Triple rinse sample container with sample before filling. Completely till the sample container with no air space. Pack the sample cooler completely In ice- The sample must be < 6.0°C upon receipt at the laboratory"' Collector's Name: Print�j��u QStyp Ag r Signature - TOXICITY TEST INFORMATION Test Required: ® Chronic (7 (lays) ❑ Acute (24-48 hours) IWC. Comments: Test Organism: ❑ Ceriodaphnla dubla (water flea) W] Plnrephales prome/as (fathead minnow) ❑ Mysidopsis bahla (shrimp) Test Concentrations (if multiple dilutions) - SHIPPING INFORMATION Reitnquished Date: Time: l PM Received by. Date Time: (�AMS PM Relinquished by Date: f7 Time: 15-T7 7 AM Received by. Date: Time: AM PM Relinquished by: Date: Time: AM PM Received by: Date: Time: AM PM Relinquished by: Date: Time: AM PM Received by Date, Time: AM PM Sample Temperature (°C).- Method °C):Method of Shipment: ❑ UPS 0 Fed EX ❑ Meritech Pick-up ❑ Delivered [] Other Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED " SAMPLE RECEIVING (Laboratory Use Only) Relinquished by: Received by: Sample Temperal e Date: Time , 7 �" AM PN Sample Condition. WHiTE = Laboratory copy YELLOW = Client copy �� MERITECH, INC, MerltechSamplell)#• Bioassay Sample Chain of Custodv ip;- *' 642 Tamco Rd, Reidsville NC 27320 Phone: 1-336-342-4748 Fax: 1-336-342-1522 Toxicity Supervisor email• mike reed(cBmerltech-labs.com Web Site: www.meritech-fabs cam ,, _ CLIENT INFORMATION Client: 1cvyj yJ C) Q U-Q.-rrjgc►`N PO#: Contact Person: '?c'.' `(- h i k e-ri NPDES#: NC OOD Z el 5 Address: Z Zo 4 a � rhe `e • � Phan .-1 4 City: ©c' \'k% ,1 Pipe Q4b-'+ County: _ �cy�,�t�) Sm� State: I Zip: { SAMPLE INFORMATION Sample Slte: 5f� Sample Type: ❑Grab ® Composite # of containers: 2 - Sampling Sampling Time: Start Date: (4 l && C 1,71 Start Time. t3 `. C]v ® PM EndDate:-512:1 I m- End Time: v • cap. PM *'* Triple rinse sample container with sample before filling. Completely rill the sample container with no air space. Pack the sample cooler completely in lice. Thesamplemust be < 6 0°C upon receipt at the laboratory'' Collector's Name: Prink Sf tf- tt le, \ iJ CjC,` �-� Signature: fz� ax & -t -'L -r TOXICITY TEST INFORMATION Test Required: Chronic (7 days) Test Organism: © Ceriodaphnfa dubla (water flea) ❑ Acute (24-48 hours) ❑Q Pimephales promelas (fathead minnow) ❑ Mysidopsls bahia (shrimp) liruC: 0-1 y % Test Concentrations (if multiple dilutions). Comments: SHIPPING INFORMATION Relinquished by-_V4'-k_Q( }- Dale: �� 2 I I I' Received by / Date: - Z% --/•7 Relinquished by: liL Dater Received by: Date: Relinquished by: Received by: Relinquished by: Received by: Date: Date: Date: Date: Sample Temperature (°C): Time- l G 4 L a PM Time: 0 ,0 L AQ PM Time: _ ri/ 7 AM PM Time: AM PM Time: AM PM Time: AM PM Time, AM PM Time: AM PM Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑ Delivered ❑ Other '* Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday detiver% NO SIGNATURE REQUIRED SAMPLE RECEIVING (Laboratory Use Only) Relinquished by:� i Received by: Date: Time: AM QMH , j v Sample Temperatures (°C)• / 7 Sample Condition: WHITE = Laboratory copy YELLOW = Client copy Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 05/04/17 Facility: TOWN OF BENSON WWTP NPDES#: NCO020389 Pipe#: 001 County-JOHNSTON Laboratory Performing Test: MERITECH LABS, INC. -0.1667 Reduction: -2.830 CONTROL 905.1- Effluent # Replicates 12 12 Female Live 12 12 Adult Male 0 0 Adult Dead 0 0 Adult Mortality 0.00% 0.00a ## Neonates 247 254 Mean # Neonates 20.583 21.167 Standard Deviation 2.610 2.443 Coefficient of Variation 12.679% C Fisher's Exact Test A = 12 B = 12 a = 12 b = 12 a/A = 1.00 b/B = 1.00 Success is: survival Critical b value = 8 12 > 8 The test concludes that the proportion of survival is not significantly different for the control and the effluent groups. Test Passes! SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA ORDERED OBSERVATIONS i Group Neonates Centered i Group Neonates Centered 1 E 16 -5.1667 13 E 21 -0.1667 2 C 17 -3.5833 14 E 21 -0.1667 3 C 18 -2.5833 15 C 21 0.4167 4 C 18 -2.5833 16 C 22 1.4167 5 E 19 -2.1667 17 C 22 1.4167 6 E 19 -2.1667 18 E 23 1.8333 7 C 19 -1.5833 19 E 23 1.8333 8 C 19 -1.5833 20 C 23 2.4167 9 C 19 -1.5833 21 E 24 2.8333 10 E 21 -0.1667 22 C 24 3.4167 11 E 21 -0.1667 23 E 25 3.8333 12 E 21 -0.1667 24 C 25 4.4167 SHAPIRO-WILIC'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.) COEFFICIENTS AND DIFFERENCES i x(n-i-1) x(i) a(i) x(i) 1 4.4167 -5.1667 0.4493 9.5834 2 3.8333 -3.5833 0.3098 7.4166 3 3.4167 -2.5833 0.2554 6.0000 4 2.8333 -2.5833 0.2145 5.4166 5 2.4167 -2.1667 0.1807 4.5834 6 1.8333 -2.1667 0.1512 4.0000 7 1.8333 -1.5833 0.1245 3.4166 8 1.4167 -1.5833 0.0997 3.0000 9 1.4167 -1.5833 0.0764 3.0000 10 0.4167 -0.1667 0.0539 0.5834 11 -0.1667 -0.1667 0.0321 0.0000 12 -0.1667 -0.1667 0.0107 0.0000 1 W = X 137.2618 140.5833 Calculated W = 0.976 Critical W = 0.884 0.976 a 0.884 The reproduction data is normally distributed evaluated at a 99t confidence interval. Test Passes! F test for Homogeneity of variance Control variance 6.8106 F = _ = 1.14 Effluent variance 5.9697 Numerator degrees of freedom: 11 Denominator degrees of freedom: 11 Critical F = 5.32 1.14 s 5.32 =► The Test PASSES, the variances of the two groups are significantly the same, homogeneous. "Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date 5/4/2017 Facility Town of Benson NPDES # NC00 20389 Pipe #: 001 County: Johnston LaboratofAmeriwinc. 42Comments Signature of Operator i Respons•b Cha X-- Signature of Laboratory Supervisor MAIL ORIGINAL TO. Water Sciences Section I Aquatic Toxicology Breneh ( DDC'. O Division ofWaterflesources 1621 Mail Service Center Raleigh, N.C. 27699-1621 Test Initiation Date/Time 4/25/2017 4/27/2017 3:41 PM Irk Avg WUSurv. Control -0.89-4-1 Test Organisms % Eff. Repl. 1 2 3 4 59 F Cultured In -House Control I Surviving # 10 10 10 10 % Surviva[F-70-001 lw Outside Supplier Original # 10 10 10 10 Wi/original (mg) 0.890 0.838 0.989 0 858 Avg Wt (mg) -0.8-94---1 Hatch Date: 4124/17 2-2-5-1 Surviving # 8 10 10 10 % Survival 1 95.0 Hatch Time 3.00 pm CT Original # 10 10 10 10 Wt/original (mg) 0 623 0.871 1.001 0.983 Avg Wt (mg) 0.870 F-47-1 Surviving # % Survival 100.0 10 10 10 10 Original # 10 1010 10 WNoriginal (mg) 1.026 0.3724 1 029 1 0.945 1 Avg Wt(mg)l 0.968 75 Surviving # % Survival 97.5 10 10 10 9 Original # 10 10 10 10 Wiforrginal (mg) 0 898 0.958 0.957 1 0 821 1 Avg Wt (mg) 0.909 90 Surviving # % Survivall 100.0 10 10 10 10 Original # 10 10 10 10 Wt/original (mg) 0.933 0,950 1 0 894 0.953 Avg Wt (mg) 0 933 i 00 Surviving # % Survival 100.0 10 10 10 10 Original # 10 10 10 110 Wt/original (mg) 1.032 0.897 0.929 0.871 Avg Wt (mg) 0 932 Nater Quality Data Day Control 0 1 2 3 4 6 6 pH (SU) Init/Fin DO (mg/L) lnit/Fin Temp (C) Init/Fin High Concentration pH (SU) iniUF!n DO (mg/L) Init/Fin Temp (C) Imt/Fn Sample Collection Start Date Grab Composite (Duration) Hardness (mg/L) Alkalinity (mg/L) Conductivity (umhos/cm) Chlonne(mg/L) Temp. at Receipt (°C) 8.01 / 7 64 8.03 1 7.79 8.09 1 7 70 8.15 / 8.03 8.16 / 8.00 8.20 1 7 8418 20 1 7 62 7.78 / 6.74 7.45 / 6.23 8.07 1 6 09 7.82 1 7.13 8.20 1 7.90 8.15 1 6.09 7 72 1 6.29 241 / 24 5 24.0 1 24.7 24 3 1 24.8 24.0 1 25.0 25.5 / 24.2 24.0 / 24.2 124.5 1 25.1 a 1 2 3 4 6 8 7.03 / 7.39 7.64 / 7.91 6.83 / 7 27 7 07 1 7 66 7.61 1 7 74 7.74 / 7 47 17 30 1 7.48 7.90 18 38 7.76 / 6.10 8.27 1 6 25 8 12 1 7.02 8.30 1 8.00 8.15 / 6.75 17.00 1 7.03 25.1 / 24.8 24 5 1 24.9 24.2 1 24.0 24.9 1 25.2 1 25.4 1 24.4 124.1 / 24.0 124 3 1 24 2 1 2 3 4/24/2017 4/26/2017 4/27/2017 Normal Irk Irl 24.0 24.0 24.3 60 30 31 59 24 27 286 1 193 212 <0.1 <0.1 <0.1 22 24 1.8 Dilution H2O Batch # 1152 1153 1154 1156 1.186 Hardness (mg/L)42 46 42 46 44 Alkalinity (mg/L) 53 53 50 51 54 Conductivity (umhos/cm) 156 207 191 192 200 Overall Result ChV >100 Stats Survival Growth Normal Irk Irl Horn. Var. n [P NOEC 100 100 LOEC >100 ?100 ChV >100 >100 Method Steers Dunnetrs Overall Result ChV >100 Stats Survival Growth Cone. Critical Calculated Critical Calculated 22.5 10 16 2.41 0.3745 45 10 18 2.41 -1.1466 75 10 16 2.41 -02278 90 10 18 2.41 -0.5984 100 i 0 18 2.41 -05945 Meritech, Inc. Chronic Fathead Minnow Benchsheet Transfer and Feeding Dates and Times Client- Benson # of Organisms per Chamber. 10 NPDES #: 0020389 Initiated by:!<"r`– L Date/rime Fed: 4/25/17 1,30r,-, Date/Time Born: 4/24/17 3;00 PM Cr Organism Source: Aquatox, Inc. Test Vessel Size: 400 ml Test Solution Volume: 250 ml Temp. of Stock: �i 1 °G # of Reps: 4 Test Organism: Pimephales_ promelas Start Date: 4/25/17 Time: End Date: 5/2/17 Time: Randomization: Yes No I ncubator 4: /— Fed by: Analyst(s): MR, CD, LV, SB Transferred by: Time 1 Initials Date Time Initials Day 1 4,(26 1 �� n"tJ ,� % " Day 2 4/27�,*, Day 2 Day 3 4/28 f� Day 4 4/29 �'+ Day 5 4/30 4/29 ', '- Day 6 511 _, (✓V Test Vessel Size: 400 ml Test Solution Volume: 250 ml Temp. of Stock: �i 1 °G # of Reps: 4 Test Organism: Pimephales_ promelas Start Date: 4/25/17 Time: End Date: 5/2/17 Time: Randomization: Yes No I ncubator 4: /— Fed by: Analyst(s): MR, CD, LV, SB Test Termination Data: Initials/Signature: End Date- 5/2/17 Date Time 1 Initials Time 2 Initials Day 0 4/25 q.' j 0�gt Day 1 4/26 s . 05Ag ,t C Day 2 4127 -VA-;A Day 3 4/28 e Lie Day 4 4/29 ', '- S Day 5 4/30 rj �Y �''�� (/O Day 6 5/1 1_-,v�t� — ,- l'" <� Test Termination Data: Initials/Signature: End Date- 5/2/17 Client: Benson MAEM/TAECH'01 //VC_ ENVIRONMENTAL LABORATORIES A Dlvision of Water Technology and Ccw; trots, Inc_ Chemical and Physical Determinations NPDES#: OQ40389 Start Date: 4/25/17 End Date: 5/2/17 Day Concentration; conti-ol 0-1 11 1 2-3 3-4 4-5 5-6 6-7 Remarks Soft Synthetic Feshwatei Batch # ; � j � initial 11-154;7 7� � 7,7 � 1 J ! pH: Initial Q . / 5�,5 / 3 r , v • (c Final I �` D.O.: c"3" Sri ��I Y4 �7. 42 D.O.: Initial '7 fj��'8IN , �) i I ?'/' Final ,2l h C) 9 7, 7r 7--' (,( _ Temp.: Initial 4 r % ), t D Z2 J.• � , S 2 - / ,�f_ • `7 Final v�`�, �-•'? q �, k 9!; ` H, ;,Z .2f&i I ' Conductivity: Initial �� (� , ;i1 - g 1 `1 Final (Fl j 1f3- 4fA --Z a L a I S 1ri Residual Chlorine: , a Lr� Z -0t Z.v Hardness: LA Q -------� Alkalinity: 1 572 S 3 t) - S S' Day Concentration: 22.50% 0-1 12 2-3 34 4-5 5-6 6-7 Remarks H• initial -7, -7 7� � 7,7 � 1 r � 5r Final . Final 5�,5 / 3 • (c �-- Initial 7-5 D.O.: Initial 7 7 �� , r 7 S $,� fj��'8IN d"` (f, Final ' I� Temp 7. !ti-' , 7`-j •i -S Temp.: Initial Lj . .fj Final Conductivity: Initial ` a 0 3 Conductivity: Initial 7 ;i1 R6 1 613 1 `1 Residual Chlorine: Final e_ui 4fA CC L a I S 1ri a Residual Chlorine: G Z.v Day Concentration: 45.0% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks PH: Initial 0 �j 7� � `7� � 1 Final . a D.O.• Initial nj? ! c` d - Final 1 d"` (f, .o i 2rQ Z, 7 Temp Initial j, "(` ' k % � `� 5 Final Conductivity: Initial ` a 0 3 Final Residual Chlorine: L ut e_ui 4fA z < l ME'R/'TE'CHr /NC ENVIRONMENTAL LABORATORIES A Ol.�/s/ora cl WIIC .' �1Tcnno/epy »nd Controle. InC Chemical and Physical Determinations Page 2 of 2 Client: Benson BIPDES#: 0020389 Start Date: 4/25/17 End Date: 5/2/17 Concentration: 75% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pia: initial 7- -7r 1 3 7 7- 7 el 7t kl sit 7 Final q 3 % i ' 7i Y$I AO- l -7. 49 D.O.: initial $, fp D.O.: D.O. Initial -7- `1 Lo Y FO,;Ut Final c U j L . 6k r Final r Temp.: Initial �7 oZ`�-{� 7 7:3 a �'�'� 2 ?tfAC Ll I) Final 1.� a r S � t �? ;t � -U . Final Conductivity: InitialFinal- oZLI, 6 1-7C( _�,4. t(. }� Conductivity: Initial i 19 qi'll pill 7QS� 92- a3 Residual Chlorine: L-uZ L.ut j 91 Gut ?,l p Concentration: 90% 0-I 1-2 2-3 34 4-5 5-6 1 6-7 Remarks H: Initial '7, 1(. `,, 6. �1 sit 7 7.7 0, Final 311) ,' 1 Yd- -7. 49 D.O.: Initial `7 fp D.O.: D.O. Initial -7- `1 Lo Y FO,;Ut _ S-, D 1 S, ! 3 , r©- Final r Tem Initial, 7.05-- 7 7:3 lq, -6 Temp. Initial Ll I) Final ',9tl. -5 t ;�q ?, `T .(} Final Conductivity: Initial oZLI, 6 1-7C( _�,4. t(. }� Conductivity: Initial M3 _g 3)_0� 7QS� 92- a3 Residual Chlorine: Final 91 '3(14 3(14 ?,l R p Hardness- Residual Chlorine: Z ot. j Lw ` l ZO4 V I -&,1 G.va,L zfui nail Concentration: 100% 0-1 I2 2-3 3-4 4-5 5-6 1 6-7 Remarks pH: Initial 7 Final 0, qTT _2,44 71 i -7. 49 D.O.: Initial `7 fp t 3a Final ,'3`r� ,' r©- �Cr 7. 03 Tem Initial, �t S lq, a �l J L L, Ll I) Final 0?`1 t eP , 0 -A .(} �fs'�-- Conductivity: Initial 3 �l 1-7C( A XA }� Final l _g �`!' l c1 92- a3 Residual Chlorine: Zu ,, p Hardness- Alkalinity: MERITECH, INC. Mortality Data: Chronic Fathead Test Client: Benson NPDES#: 0020389 Start Date: 4/25/17 End Date: 5/2/17 Concentration Control Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 V J� a J e3Rep B 0 Q `"_ Rep C 0_ Rep D 0 v Concentration 22.50% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 Rep B 0 Rep C 0 l� / Rep D 0 Concentration 45.0% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday ed Rep A 0 lJ 0 1") CD Rep 0 U n _! V Rep C 0 0 Rep D 0 � ;D _`� MERITECH, INC. Mortality Data: Chronic Fathead Test Client: Benson NPDES#: 0020389 Start Date: 4125117 End Date: 5/2117 i Concentration 75% i Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday ` Rep A 0 ed l/` _ / [O 1 Rep B 0 Rep C 0 0 Rep D 0� C/ " Concentration 90% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday O Rep A 0_ Rep B 0 Rep C 0 Rep D 0 —r—_ Concentration 100% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 Rep B 0 Rep C 0 0/ Rep D 0 `. �/t _ a lff,AERI TAECM,. MIC. ENVIRONMENTAL LABORATORIES Qy A Dlvlsion or Water 7iehnology and Conry/s, Inc FISH LARVAL. SURVIVAL/GROWTH DATA SHEET CHRONIC FATHEAD MINNOW Facility: Benson Page: 1 of 2 NPDES #: 0020389 Outfall: Date of Test: 4/25/17 Initial weights taken on: 11776�' by: �V Organism: Pimephales aroma/as Final weights taken on: - by: CONC REP PAN WT. (mg) PAN + ORG. WT. (Mg) WT. OF ORG. (mg) # ORG. MEAN WTJORG. (mg) SURV % 0 A 3�b7 �.5 Q 6,�qd toy B F ,1�9 IS 1 91 /0 ()'R3 e 160 G � ;� U 1) 6, (1'tY f 106 D /,06 22.50% A 1. 3>37-93 - B 37, S,t6. "� 3,71 (�0,3-71 lea G -3 A 6 /. as 1 166 fl 8`51, �7 Y3 a• qe3 la 45.Q% A I �,1'77 e53.63 a 0� baa B 8T'2�.. )6 ,7a % 6-a7J � G 3LI,� D Fqq, q I 353..E . 'L/S �� �- f �SI 12 HR1 Y HC"j1 11vc. ENVIRONMENTAL LABORATORIES A Divfsfon of WaTb-ch—logyTb-ch—logy and Contrafs. /nc tes' FISH LARVAL SURVIVALIGROWTH DATA SHEET CHRONIC FATHEAD MINNOW Facility: Benson NPDES #: 0020389 Date of Test: 4125117 // Initial weights taken on: L/— 6 — 1 —]— by= LV Final weights taken on: — / by: I✓� Page: 2 of 2 Outfall: Organism: Plmephales promelas CONC REP PAN WT. (mg) PAN t ORG. WT. (mg) WT. OF ORG. (mg) # ORG. MEAN WT.IORG. (mg) SURV % 75% A �'� �� GY 106 s '' y�`' o o �� `�� loci .0-457�' /oo D Wg1,g go,0 _ � o- F ,�,) f �o 90% A 93163 Y >> q33 to (0 3) 100 B Y�Y, 0 3.5 q,�() 10 o • �5 () 100 C Ll/•P,7 E�3*l-1 c1`1 /0 o' -�>q 166 D W36, a8 3&11.e q- S 3 /0 o, q53 60 100% A Wq5,. Iq 396',S1 10'32 10 " o 3'a 100 B 9A 10 ��`/ R '� ., aq'7 0 - �? 9 9U7.C " a 1 0,T71 loo MERITECH, INC. MeritechSample 104P 01f:�6)7 7C Bloassay Sample Chain of CustoA& IPW dy 642 Tamco Rd, Reidsville NC 27320 Phone: 1-336-342-4748 Fax: 1-336-342-1522 Toxicity Supervisor email: mike.reeddilmeritech-labs.com. Web Site- www.meritech-labs.com CLIENT INFORMATION Client: �l,y) t� d �'- PO#: Contact Person: t1 - NPDES#: NC ZC7 Address: 10 6,,3 Pho4 --Z 1 City: 0.h Pipe #: no l County: YjyJ �,C�1N State:? Zip.= SAMPLE INFORMATION Sample Site: Sample Type: ❑ Grab Composite # of containers: Sampling Time. Start Date: �[ IZ,) tt t 1i Start Time: _ 61 . LS SAM PM End Date: 41 q I i ? End Time: G -,-Tc-) PM Triple rinse sample conialner with sample before rrlling. Completely Hit the sample container with no air space. Pack the sample cooler completely in Ice. The sample must be < 60C upon receipt at ,t/h1e laboratory`*" Collector's Name: Print: _�i' r k c. + �� Signature. -fA lJt- L Test Required: '9 Chronic (7 days) Comments; ❑ Acute (24-48 hours) IWC: —SEG % TOXICITY TEST INFORMATION Test Organism: [p Cerlodaphnis dubta (water flea) 0 Pimephales promelas (fathead minnow) ❑ Mysidopsts bahla (shrimp) Test Concentrations (if multiple dilutions): `7►'' SHIPPING INFORMATION Relinquished by: .� � V1v Date oZ �� 7 Tune- AM PM Received by: -'�-- — Date Pyla 8 l/I % Relinquished b ,% '� Date: T�.� / / 7 ❑ Delivered ❑ Other Received by: Relinquished by. Received by: Relinquished by: Received by Method of Shipment:❑ UPS Date: D ate: Date: Date: Dale: Sample Temperature (°C): ❑ Fed EX ❑ Meritech Pick-up Time: /Q�'{� AM PM Time: AM jPM / Time: AM PM Time: AM PM Time: AM PM Time: AM PM Time: AM PM " Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED SAMPLE RECEIVING (Laboratory Use Only) Relinquished by: Received by: Date- `�f '�®! 17 Time: 3L �� AMM Sample Temperatures ('C): / I / / Sample Condition: QA i Lt, ��► WHITE c Laboratory copy YELLOW = Client copy Title: Benson File: BensonGrowth Transform: NO TRANSFORMATION Shapiro - Wilk's Test for Normality ---------------------------------------------------------------------------- D = 0.1510 W = 0.9355 Critical W = 0.8840 (alpha = 0.01 , N = 24) W = 0.9160 (alpha = 0.05 , N = 24) ---------------------------------------------------------------------------- Data PASS normality test (alpha = 0.01). Continue analysis. Title: Benson File: BensonGrowth Transform: NO TRANSFORMATION Bartlett's Test for Homogeneity of Variance ---------------------------------------------------------------------------- Calculated B1 statistic = 9.0011 (p -value = 0.1090) Data PASS B1 homogeneity test at 0.01 level. Continue analysis. ---------------------------------------------------------------------------- ' Critical B = 15.0863 (alpha = 0.01, df = 5) = 11.0705 (alpha = 0.05, df = 5) Title: Benson NO TRANSFORMATION File: BensonGrowth Transform: ANOVA Table SOURCE DF SS _ ----- - MS F - - Between -------------5 --------------- --------- 0.0238 0.0048 0.5667 is --------n-(Error)---------------------0_1510-----------0`0084--------------- Total 23 0.1747 ------------------------------------------------------------value - 0.7244) i Critical F = 4.2479 (alpha = 0.01, df = 5,18) = 2.7729 (alpha - 0.05, df = 5,18) i Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05) Title: Benson NO TRANSFORMATION File: BensonGrowth Transform: Dunnett's Test TABLE 1 OF 2_ Ho:Control<Treatment---- --- ------------------------------- TRANSFORMED MEAN CALCULATED IN SIG GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0.05 - -------------------- ------- 1 Control 0.8938 0.8938 2 22.50 0.8695 0.8695 0.3745 3 45.0 0.9680 0.9680 -1.1466 4 75 0.9085 0.9085 -0.2278 5 90 0.9325 0.9325 -0.5984 6 100 0.9322 0.9322 -0.5945 Dunnett -critical value = 2.4100 r-(1 Tailed, alpha = 0.05, df = 5,18) Title: Benson NO TRANSFORMATION File; BensonGrowth Transform: Dunnett's Test - TABLE 2 OF 2 Ho:Control<Treatment ----------------------------------- ----------------------- NUM OF MIN SIG DIFF % OF DIFFERENCE GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL --�-- ------ Control 4 2 22.50 4 0.1561 17.5 0.0242 3 45.0 4 0.1561 17.5 -0.0743 4 75 4 0.1561 17.5 -0.0148 5 90 4 0.1561 17.5 -0.0388 6 100 4 0.1561 17.5 -0.0385 -------------------------------------------------- Title: Benson File: BensonSury Transform: NO TRANSFORMATION Shapiro - Wilk's Test for Normality ---------------------------------------------------------------------------- D = 0.0375 W = 0.6408 i Critical W = 0.8840 (alpha = 0.01 , N = 24) W = 0.9160 (alpha = 0.05 , N = 24) j------------------------------------- - Data FAIL normality test (alpha = 0.01). Try another transformation. Warning - The first three homogeneity tests are sensitive to non -normality and should not be performed with this data as is. Title: Benson File: BensonSury Transform: NO TRANSFORMATION Hartley's Test for Homogeneity of Variance Bartlett's Test for Homogeneity of Variance ----------------------------------------------------------------------------- These two tests can not be performed because at least one group has aero variance. Data FAIL to meet homogeneity of variance assumption. Additional transformations are useless. Title: Benson File: BensonSur'v' Transform: NO TRANSFORMATION Steel's Many -One Rank Test - Ho: Control'<Treatment ---------------------------------------------------------------------------- MEAN IN RANK CRIT. SIG GROUP IDENTIFICATION ORIGINAL UNITS SUM VALUE DF 0.05 ------------------------- 1 Control ----------- 1.0000 ------- ------ ----- --- 2 22.50 0.9500 16.00 1.0.00 4.00 3 45.0 1.0000 18.00 10.00 4.00 4 75 0.9750 16.00 10.00 4,00 5 90 1.0000 18.00 10.00 4.00 6 100 1.0000 18.00 10.00 4.00 ---------------------------------------------------------------------------- Critical values are 1 tailed ( k = 5 ) Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 02/02/17 Facility: TOWN OF BENSON WWTP NPDES#: MC0020389 Pipe#: 001 County: JOHNSTON to Perming est : E - ETH LABS, INC. \) Comments v X Signature -of Laboratory -Supervisor I * PASSED: -0.35% Reduction Work Order: Environmental Sciences Branch MAIL ORIGINAL TO: Div. of Water Quality N. C. DENR 1621 Mail Service Center Raleigh, North Carolina 27699-1621 Chronic Pass/Fail Reproduction Toxicity Test �ONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 # Young Produced 1126127122123126120125125124121123126 Adult (L)ive (D)ead JAL IL IL IL IL IL IL IL IL (L IL IL effluent 90% 'REATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 # Young Produced 1124122126123127126125124122122127121 Adult (L)ive (D)ead IIL IL IL IL IL IL IL IL IL IL IL IL Chronic Test Results Calculated t = -0.094 Tabular t = 2.508 Reduction = -0.35 Mortality Avg.Reprod. 0.00 24.00 Control Control 0.00 24.08 Treatment 2 Treatment 2 Control CV a 9.2320 PASS FAIL o control orgs X producing 3rd r 0 brood CheckEon, 100 0 o 1st sample 1st sample 2nd sample Complete This For Either Test pH Test Start Date: 01/25/17 Control 8.10 8.17 8.18 8.09 8.12 7.92 Collection (Start) Date Sample 1: 01/23/17 Sample 2: 01/25/17 Treatment 2 7.62 8.05 7.57 8.08 7.83 7.85 Sample Type/Duration 2nd 1St P/F s s s Grab Comp. Duration D t e t e t e I S S a n a n a n Sample 1 X 24.0 hrs L A A r d r d r d U M M t t t Sample 2 X 23.3 hrs T P P 1st sample 1st sample 2nd sample D .O. Hardness (mg/1) 42 .....:::......... Control 8.20 8.01 8.17 8,05 8.12 7.51 Spec. Cond.(pmhos) 157 341 379 Treatment 2 8.64 8.02 8.54 8.00 8.22 7.53 Chlorine (mg/1) , <0 . 1 <0.1 LC50/Acute Toxicity Test Sample temp. at receipt(OC) 0.2 1.8 (Mortality expressed as %, combining replicates) 6 o a Ra a r o 6 0 r 0 0 % % o % a "s o % a % Note: Please Concentration Complete This Section Also Mortality start/end start/end Control High r --- PH D. 0. GC50 = % Method of Determination 950 Con a�enae Limits Moving Average Probit -- o Spearman Karber - Other Organism Tested: Ceriodaphnia dubia Duration(hrs): Copied from DWQ form A'1'-1 (3/87) rev. 11/95 (DUBIA ver. 4.41) Meritech, Inc. Mini Chronic Pass/Fail Test. Cedbdaphn/a dubia a�J Incubator #: Client: P to 5011 Pipe #: � County: off . Date Start: % - � 1 % Date End: NPDES #: NC 00a0 3 9'9 Date / Time of Culture Transfer: 1 ' _vtM Time Start:�7i;U� �Time End. Dilution Water. Lake Brandt Date / Time Neonates bom: 1. >h- m 1st Renewal Date: _/- a7—/ `� Time- Test Organism Source: Tray # Age of eonates at Test Start �ai� , hours 2nd Renewal Date: j - 2,ZI •-1'1 Time: CJ: (,¢M Stirred / Aerated for D.O.: Y / N Randomized./ N Culture Tray Tempe °C Analyst(s): MR, CD, LW, se Reviewed by: f,.v...-- r)av 09 1 9 :1 4 5 6 7 8 9 10 11 12 #Young Produced 0 0 0 0 0 0 0 0 0 0 0 0 Adults Live I Dead L-. Z-- G- L G- L G Z L L... L L.-. Dav its 1 2 3 4 5 6 7 8 9 10 11 12 #Young Produced 1619 1 q/11 131115/11VI 1 0 1 0 IM 141115 0 3141WE 1 0 5/ Adults Live I Dead Adults Live I Dead L- L-- I /--11-- I /L I L I L L- I Z - t- I L- �- I /L QM#7 1 2 3 4 5 6 7 8 9 10 11 12 #Young Produced Adults Live / Dead I L Total Produced 12 LU'j P-3 1 x 1_Pd? -Id`LY I o;11 Ic Percent of Control producing third brood: Test Sample Organism Reproduction Effluent %: . Dav 92 1 2 3 4 5 6 7 8 9 10 11 12 # Young Produced 0 1 0 1 0 1 0 1 0 1 0 1 0 1 0 0 1 0 1 0 0 Adults Live I Dead /i L- Z— L- I L I /L I L I L L- I Z - I L L_ Dav #5 1 2 3 4 5 6 7 8 9 10 11 12 # Young Produced I I /Q I 1 / Sample 1 %l 1 111 /l Transfer Day Y/10 I Ve 1,7/7 I L // 3 Adults Live / Dead L.- LjpxY _rE �,., L. G- L G- G— t - Day #7 1 2 3 4 5 6 7 8 9 10 11 12 #Young Produced I I /Q I 1 / Sample 1 %l 1 111 /l Transfer Day Adults Live / Dead Sample 2 L I L i- I L I L IC, fir i • _ •. -. [rr�1��".t..��w%►JCS..:rtG���i�A■il�7�I�.��.�7���'%�i, Comments: Sample 1: �I'R 2-17 Sample 2: Sample Information Batch # 100% pH G ! C? Duration Sample 1 �j,,7L Transfer Day hours Sample 2 5E G hours Batch # Q. �o �t4Sample ! Sample 2 Transfer Day 0 2 5E 6�V a w Hardness �r, LjpxY _rE (m9/L) Spec. Cond(umhos/cm) 7-11 7G1 Chlorine r -i.—. Irr fir, .r arc 11 fo t ` Receipt SamplekS"� Temp. Co) ransferred by. Fed by. Day 0 L -V Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 LIZ Terminated by -"l'~ pH 1st Sample 2nd Sample 2nd Sample Control �,30 �o Sample 10- lmdal mal nlaa final in-itial final D.O. 1st Sample 2nd Sample 2nd Sample Control �,30 �o Sample Jrbq $:2�d 25 -inifial final ml a ring Mnla final Temp. 1 at Sample 2nd Sample 2nd Sample P -M 256 V11,11 L'IP b /yr 04 mrtia nnal ,n ru final rmlra final Control 12. Samples lq,4 P -M 256 V11,11 L'IP b /yr 04 mrtia nnal ,n ru final rmlra final MERITECH, INC. Meritech Sample 113* Bloassav Sample Chain of Custody 642 Tamco Rd, Reidsville NC 27320 Phone: 1-336-342-4748 Fax: 1-336-342-1522 Toxicity Supervisor email: mike.reed@meritech-labs.com Web Site www.meritach-labs.com CLIENT INFORMATION Client �:•sc'•,t� Contact Person: V7.,,o- Address: `Z ,xy �A ckyJ gq&A&N County: 1L State: 013'11113T PO#: NPDES#-. NCC43 Z 0L:�-gq Phone: Pipe #: G3t1 Zip: 7'15 2 U SAMPLE INFORMATION Sample Site: SampleType: ❑ Grab Composite # of containers: Z, Sampling Time: Start Date: S� '� t )"t Start Time: 0% : ° PM End Date: L i -LA r�, End Time:7 PM *** Triple rinse sample container with sample before filling. Completely rill the sample container with no air space. Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory*'' Ob A Pin Collector's Name: Print: li " G 1�Ufa-ii�"CY ✓ Signature: _ T i Test Required: 91 Chronic V days) ❑ Acute.(24-48 hours) IWC: c3 % Comments: TOXICITY TEST INFORMATION Test Organism: 9 Ceriodaphnia dubia (water Flea) - -_ - ® Phriephales promelas (fathead minnow) ❑ Mysidopsis bahla (shrimp) Test Concentrations (d multiple dilutions): SHIPPING INFORMATION Relinquished by: Date: - /7 "Time: CP..�''r� M PM Received Date. Z p t/ , % 7 Time: w � PM Relinquished by: Date- / 24' ' / 7 Time: :/' AMPM Received by: Date. Time: AM PM Relinquished by: __ Date Time: AM PM Received by: Date: Time: AM PM Relinquished by' Date: Time: AM PM Received by: Date. Time: AM PM Sample Temperature (°C): Method of Shlpment.❑ UPS ❑ Fed EX ❑ Meritech Pickup ❑ Delivered ❑ Other *" Samples shipped on Friday must be FedEx -and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED " SAMPLE RECEIVING (Laboratory Use Only) Relinquished by: C"1 _ ? Received by. l Date: 1-34-17 Time:1-1 � A PM Sample Temperatures (°C): G, Sample•Condiflon. r WHITE = Laboratory copy YELLOW = Client copy MERITECH, INC, Meritech Sample ID* Bioassay Sample Chain of Custody 642 Tamco Rd, Reidsville NC 27320 Phone: 1-336-342-4748 Fax 1-336-342-1522 Toxicity Supervisor email: mike.reedfd_-meritech-labs.com Web Site: www.merifech-labs.com CLIENT INFORMATION Cflent:_1i�t�ilJ p� Q5>:y�(2iJd PO#: CL L Contact Person ��mtv 1 Cuv NPDES#:f N� n SS Address:— �� t,�c4ltt" �n �n 6tr�� �' ds -A - Phones 1�� �S ti _ 2- City:��a.3 C) Pipe .- County: _),A,,9,-3 S 0't3 State: (I V - zip: SAMPLE INFORMATION Sample Site: Uo-� i Sample Type: []Grab Composite # of containers: Sampling Time: Start Date: !. i'LS _ 1"Z Start Time: PM EndDate: A2-tj End Time: n2�- 3 rte- .P PM *"* Triple rinse sample container with sample before rdling. Completely fill the sample container with no airspace. Pack the sample cooler completely In ice. The sample must be < 6.0°C upon receipt -at the laboratory' Collector's Name: Print�Signature: tsr� �� 4"/?@t. TOXICITY TEST INFORMATION Test Required: M Chronic (7 days) Test Organism: © Ceriodaphnla dubia (water flea) ❑ Acute (2445 hours) Pimephales promelas (fathead minnow) ❑ Mysidopsis bahi'a (sh(mp) 1WC: �y % Test Concentrations (if multiple dilutions): Comments: A., SHIPPING INFORMATION 5�h Relinquished -by ' Date: 2C, 1-''t Time: 1� G upm, Received by r fy. �-� Date: iw /r % te%/ Time: Relinquished by: � Date• �� :k`�� / Time: ' 61 Received by: Date: Time: AM PM Relinquished by: Date: Time: AM PM Received by: Relinquished by: Received by: Date: Time: AM PM Date: Time" AM PM Date, Sample Temperature (°C): Time: AM PM Method of Shipment ❑ UPS ❑ Fed IX ❑ Meritech Pick-up ❑Delivered ❑ Other " Samples shipped on Friday must be FedEx*and must be clearly labeled for Saturday delivery, NO SIGNAT1lRE REQUIRED " SAMPLE RECEIVING (Laboratory Use Only) Relinquished by: �" ^ I1 r;34) r ^/ % Z Received by: �1�Q Date: ! �lq 1 / Time: IlJ 1� A PM c �1 i / :- Sample Temperatures (°C)• / t Sample Condition WHITE = Laboratory copy YELLOW = Client copy Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 02/02/17 Facility: TOWN OF BENSON WWTP NPDES#: NCO020389 Pipe#: 001 County-JOHNSTON Laboratory Performing Test: MERITECH LABS, INC. Reduction: -0.35a CONTROL 90o Effluent # Replicates 12 12 Female Live 12 12 Adult Male 0 0 Adult Dead 0 0 Adult Mortality 0.00% 0.00% # Neonates 288 289 Mean # Neonates 24.000 24.083 Standard Deviation 2.216 2.109 Coefficient of Variation 9.232% C Fisher's Exact Test A = 12 B = 12 a = 12 b = 12 a/A = 1.00 b/B = 1.00 Success is: survival Critical b value = 8 12 > 8 The test concludes that the proportion of survival is not significantly different for the control and the effluent groups. Test Passes! SHAPIRO-WILIC'S TEST FOR NORMAL DISTRIBUTION OF DATA ORDERED OBSERVATIONS i Group Neonates Centered i Group Neonates Centered 1 C 20 -4.0000 13 C 24 0.0000 2 E 21 -3.0833 14 E 25 0.9167 3 C 21 -3.0000 15 C 25 1.0000 4 E 22 -2.0833 16 C 25 1.0000 5 E 22 -2.0833 17 E 26 1.9167 6 E 22 -2.0833 18 E 26 1.9167 7 C 22 -2.0000 19 C 26 2.0000 8 E 23 -1.0833 20 C 26 2.0000 9 C 23 -1.0000 21 C 26 2.0000 10 C 23 -1.0000 22 E 27 2.9167 11 E 24 -0.0833 23 E 27 2.9167 12 E 24 -0.0833 24 C 27 3.0000 SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.) COEFFICIENTS AND DIFFERENCES i x(n-i-1) x(i) a(i) x(n-1-1) - x(i) 1 3.0000 -4.0000 0.4493 7.0000 2 2.9167 -3.0833 0.3098 6.0000 3 2.9167 -3.0000 0.2554 5.9167 4 2.0000 -2.0833 0.2145 4.0833 5 2.0000 -2.0833 0.1807 4.0833 6 2.0000 -2.0833 0.1512 4.0833 7 1.9167 -2.0000 0.1245 3.9167 j 8 1.9167 -1.0833 0.0997 3.0000 { 9 1.0000 -1.0000 0.0764 2.0000 10 1.0000 -1.0000 0.0539 2.0000 11 0.9167 .-0.0833 0.0321 1.0000 12 0.0000 -0.0833 0.0107 0.0833 1 W = X 96.5593 102.9167 Calculated W = 0.938 Critical W = 0.884 0.938 z 0.884 The reproduction data is normally distributed evaluated at a 990 confidence interval. Test Passes? F test for homogeneity of Variance Control variance 4.9091 F = _ = 1.10 Effluent variance 4.4470 Numerator degrees of freedom: 11 Denominator degrees of freedom: 11 Critical F = 5.32 1.10 s 5.32 = ► The Test PASSES, the variances of the two groups are significantly the same, homogeneous. EQUAL VARIANCE t TEST 24.0 - 24.1 t = = -0.094 0.883 Degrees of freedom = 22 Critical t = 2.508 -0.094 < 2.508 Test passed. There is not a significant difference in reproduction between the Control and the effluent evaluated at a 990 confidence interval. Chronic Test PASSES Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:212/2017 Facility• Town of Benson NPDES # NC00 20389 MAIL ORIGINAL TO: Environmental Sciences Branch Division of Water Quality NC DENR 1621 Mail Service Center Raleigh, NC 27699-1621 Pipe #: 001 County: Johnston Test Initiation Datefrime 1/24/2017 8.17 1 7.95 5:35 PM 823 1 8.05 Avg Wt/Surv. ControIF___0_.9_3_7___j Test Organisms % Eff. Repl. Control Surviving # 1 2 3 4 % Survival 95 _0__1 r -i Cultured In -House IVV. Outside Supplier 10 10 1 9 1 9 Original # 10 10 10 10 0.8 Wt/original (mg)0.936 0.952 0 825 0 850 Avg Wt (mg) 0.891 Hatch Date: 1123/17 F2_23_1 Surviving # 10 10 910 % Survival 97.5 Hatch Time: 3-00 pm CT Original # 10 10 10 10 Wt/originai (mg) 0.924 0.965 0.917 0 897 Avg Wt (mg) 0.926 45 Surviving # % Survival 97.5 10 10 9 10 Orlginal # 10 10 10 10 Wt/odginal (mg) 1.062 1 132 0.904 1 0.872 Avg Wt (mg) 0.993 75 Surviving # % Survival 100.0 10 10 10 10 Original# 10 10 10 10 Wt/original (mg) 0.904 1.069 0.946 0.920 Avg Wt (mg) 0.960 90 Surviving # % Survival 97.5 10 9 10 10 Original # 10 10 10 10 Wt/original (mg) 0.946 0.964 0.823 0.955 Avg Wt (mg) 1 0.922 1 ROM Surviving # % SurvivalE 100.0 10 10 10 10 Original # 10 10 10 10 Wt/o6ginal (mg) 0 920 0.897 1.080 1.016 Avg Wt (mg) F--0 9-7-3--1 Water Quality Data Day Control 0 1 2 3 4 a pH (SU) init/Fin DO (mg/L) Init/Fin Temp (C) Init/Fin High Concentration pH (SU) Init/Fln DO (mg/L) Init/Fin Temp (C) Init/Fin Sample Collection Start Date Grab Composite (Duration) Hardness (mg/L) Alkalinity (mgiL) Conductivity (umhos/cm) Chlorine(mg/L) Temp. at Receipt (°C) 8.16 / 7.87 8.12 / 791 8.17 1 7.95 8.09 / 8.16 823 1 8.05 8.25 17.92 822 1 7.52 8 03 / 6.94 8.08 / 6.83 8.15 / 7.64 8.25 1 8.02 8 16 17.95 8.23 1 7.48 842 / 6.05 24.9 1 25.1 25.4 / 24.2 24.1 125 6 24.7 125.2 25.4 / 25.9 24.6 / 25.4 25.6 / 259 0 1 2 3 4 5 a 7.23 1 7.51 7.40 1 7.70 7.42 / 7.76 17 63 1 7.93 17 91 / 7.89 17.88 17.64 1747 1 7.43 6.22 1 6.85 8.23 / 6.91 8.37 1 7.78 8.361 8.06 8.25 / 8 05 18.19 / 7 26 8.70 1 6.08 24.3 1 25.6 25.4 1 24.9 25 4 125.9 124.9 / 25 5 25 0 / 25.8 126.3 1 25.6 25 6 124.9 1 2 3 1/23/2017 1/26M17 1/26/2017 Normal r1l FF_E,I 24.0 23.8 24.0 38 38 40 31 36 35 359 372 368 <0.1 <0.1 <0.1 0.2 1 8 0.8 Dilution H2O Batch # 1114 1115 9116 1117 Hardness (mg/L) 43 44 46 46 Alkalinity (mg/L) 54 51 49 51 Conductivity (umhos/cm) 198 200 201 190 Overall Result ChV >100 Stats Survival Growth Normal r1l FF_E,I Hom. Var. n gyri NOEC 100 100 LOEC >100 >100 ChV >100 >100 Method Steel's Dunnett's Overall Result ChV >100 Stats Survival Growth Cone. Critical Calculated Critical Calcuiated 22.5 10 20 241 -0.6363 45 10 20 2.41 -1.8498 75r-Flioo- 22 2.41 -12544 90 20 2.41 -0.5681 100 22 2.41 -1.4999 Meritech, Inc. Chronic Fathead Minnow Benchsheet Transfer and Feeding Dates and Times Client: Benson # of Organisms per Chamber. 10 Start Date: 1/24/17 NPDES #: 0020389 Initiated by: Dateirime Fed: 1/24/17 DateMme Born: 1/23/17 3:00 PM CT Test Vessel Size: 400 ml Test Solution Volume: 250 ml Temp of Stock: ;'Y 9 °C # of Reps: 4 Organism Source: Aquatox, Inc. Test Organism: Pimephales promelas Transferred by: End Date: 1/31/17 Fed by: Time, Time: ' G: 3 � 4.r1 Randomization: Yes No Incubator #: l Analyst(s): MR, CD, LV, SB Date Date Time Initials Day 1 1/25 3q fm (/p/bl) 7 Day 1/26 �y rRf Day 3 1/27 �� C Day 4 1/28 q Days 1/29 Day 6 1/30 L End Date: 1/31/17 Fed by: Time, Time: ' G: 3 � 4.r1 Randomization: Yes No Incubator #: l Analyst(s): MR, CD, LV, SB Date Time 1 Initials Time 2 Initials Day 0 1/24 "�/`� Day 1 1/25 L ✓�� �lL_ 4 Day 2 1/26��- Day 3 1/27 Day 1/28 c%'35�,,�, Day 1/29 Day 6 1/30 Test Termination Data: Initials/Signature: End Date: 1/31/17 ENVIRONMENTAL. LABORATORIES c A Drvislon of Water Technology and Contro/s /nc Chemical and Physical Determinations Client: Benson NPDES#: 0020389 Start Date: 1/24/17 End Date: 1/31/17 n—, Concentration: control 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks Soft Synthet,c Feshwate Batch # ©�' _ iF} �. ci pH: Initial ► 2✓ 17 $` j r�-? ag�- Final ' J r �f f.�s d� S:X D.O.: Initial p (,} r� y7, Final Temp.: Initial a �) •� v2 s•�3 'f-� Y Lr Temp.: Initial Final 25 - ;-q.% I gq i LO 94_ Final Conductivity: Initial 3 'P a Lt ���0,19 Conductivity: Initial jqkp 02 q9 a c� Ici `�� S Final 40, aj `�a o� If -6 'Ia6 GO.1 Residual Chlorine: LQ. 7-0 � G(j, 1 L -O. r Z-6,) Hardness: 7 Alkalinity: S14 rj j ti Concentration: 22.50% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks PH: Initial ©�' _ iF} ?j �. ci Final ► `� $` j r�-? 7 D.O.: Initial Final Temp.: Initial a �) •� v2 s•�3 �S -� au Lr Final 25 - ;-q.% I 94_ Conductivity: Initial 3 'P a Lt ���0,19 Final(� 02 �`j a c� S 3 `�� S Residual Chlorine: 40, ,-6,1 Z-0- j I If -6 GO.1 Concentration: 45.0% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH. Initial _ �� _ 5?, 0 $, Final D.O.: Initial Final Temp.: Initial CA ;L!� -b Final 25 - ;-q.% I Conductivity: Initial ?-A?of 7� Final -a a q� u�$ Residual Clilorine: z-0 1 ,-6,1 Z-0- j I If -6 GO.1 a Client: Benson MER/TEC7 , /ju4c7_ EI�[VIRO n7MEP1TAL LA60 RATO RIES A O/v/sten �./ W Yqr abchno/ogy and Con�roJs, Jna Chemical and Physical Detea•minations NPDES#: 0020389 Start Date: 1/24/17 End Date: 1/31/17 Dav Page 2 of 2 Concentration: 75% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH: Initial 7,,5� 5D 7. t -7 Final (0� Final '�,w Q `7. � � "� w Q D.O.: Initial P, 31J %• 33 $+�',� Final IH.il • �' Final • a 5 f (� Temp.: Initial 2 - n2� , i oZ , S A- ��] - Final ' (o oZCi -� �.�r 3-S �� - �`1 J Conductivity: Initial 30.3 ?725a 1_57 •JoZO tv�� Conductivity: Initial Final S ) (o 7jLf, 1`{ b �3 Residual Chlorine: Z—Da j �fj, GliJ t "!�_b.1 G , ( L -G • l Z-0, 17-, Day Concentration: 90% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 • Remarks pH: Initial 73 7. � � ,- -7S Final '�,w Q 7, w Q D.O.: Initial P, D.O.: Initial %• 33 $+�',� 19"11 IH.il • �' Final • a 5 c Temp.: Initiala "A'%L_ �(� Temp.: Initial ��] - Final ' (o oZCi -� �.�r 3-S �� Final �`1 J `�� a (, � °Z� T tv�� �, j L_)) Conductivity: Initial �: 3 6 (o b t 5 Final %,7 3 `aq ,76 3 oZ GDJ r O 17-, Hardness: Residual Chlorine: LO. i Z-0. + Z-6 ) G 6 . Z L6.1 2-,0. ! LO, ' Day Concentration: 100% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH: Initial 7, 7. � `y% C ,- Final div 7, D.O.: Initial P, �-,37 ?W Final T ! I �' • a 5 c Temp.: Initiala d2s ,� �(� �� - ��] - Final ' (o a �t ;Z5. �� 3-S �� 1 Conductivity: Initial / 33q- 17 q `3 ? �, j L_)) Final 15q - b Residual Chlorine: y6, zD - j GDJ r O Hardness: Alkalinity: MERITECH, INC. Mortality Data: Chronic Fathead Test Client. Benson NPDES#: 0020389 Start Date: 1/24/17 End Date: 1/31/17 Concentration Control Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 Friday Saturday a Tuesday Rep B 0 Rep 0 0 f 0 0' D� Rep D 0 V 0 0 0— Concentration 22.50% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 0— Rep B 0 o Rep C 0 O Rep D 0 y _ Concentration 45.0% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 1-0 Rep B 0 0 t Rep C 0 0 l Rep D 0 MERITECH, INC. Mortality Data: Chronic Fathead Test: Client: Benson NPDES#. 0020389 Start Date: 1/24/17 End Date: 1/31/17 Concentration Irv' % Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 Friday to (/ 0 Tuesday Rep A 0_ Rep B 0_ 0 - Rep B 0_ Rep C 0 (% C) Rep D 0 CD Rep D 0 LJ `� ` 0 Concentration 90% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0_ Rep B 0_ Rep C 0 C) CD Rep D 0 LJ `� ` 0 Concentration 100% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday Rep A 0 n Rep B 0 0 Rep C 0 V Rep ! 0 _D o (J- _ ERI TEC® iric. - a ENVIRONMENTAL LABORATORIES � A Division or Water Technology and Controls, Inc. FISH LARVAL SURVIVAL/GROWTH DATA SHEET CHRONIC FATHEAD MINNOW Facility: Benson NPDES #: 0020389 Date of Test: 1/24/17 Initial weights taken on: by: L t --- Final weights taken on: ` LT by: �V Page: 1 of 2 Outfall: Organism: Plmephales,promelas CONC REP PAN WT. (mg) PAN + ORG. WT. (mg) WT. OF ORG. (mg) # ORG. MEAN WT./ORG. (mg) SURV % A _ 930 3L �l 36 1 D a. q36 100 B V C 3. LCV 993-6(8 q.-5cQ- 0 U c r� %% t I f/ L4 6i ///� - • D -o 95aLrq E. qO A F 36". o� l' sa I !♦ W 1 ry 6r1 cq DV . 7 , 3 7 q. 10 , q10 5 100 22.50%g c 1.7 q O `7 D � 93.5 9.0 7 10 ()0817 ZDD A SLI c-� In Inn 45.0% B e), R3N D Da qDq q D . (g 2356R, 7Q- lD Dn87 10 1-!- IT C v IPJC. ENVIRONMENTAL LABORATORIES A Orvision of Water 7Echno/ogv and Cont—rs, /nc_ FISH LARVAL. SURVIVAL/GROWTH DATA SHEET CHRONIC FATHEAD MINNOW Facility: Benson NPDES #: 0020389 Date of Test: 1124117 Initial weights taken on: by: Ll� Final weights taken on: 17 by: LV Page: 2 of 2 Outfall: Organism: Pimephales promelas CONC REP PAN WT. (mg) PAN + ORG, WT. (mg) WT. OF ORG. (mg) # ORG. MEAN WTJORG. (mg) SURV % 75% A 03, g'-1 W. 10 100 $ 2�• q. 3 /C/ . t o ,06q 16 Ci 2,6, 2V 4• q6 t o L/, qq6 Ion D 3 35 /--73 �/, n L oe ��� L 90%B A e3? Q 7.5P) q`L l� �' , I ���.�-7g a fo �� q�qb �.. �- �� g� -� lo D . &Z leo D �51��9 55 /0 Oa q5_ l o 100% A-5,17 gLI.3 � o ao l,0 f3 Y3 9-Y-6. Z 9,97 lo 6.-Rq:7 70r) 7111,96 10 D f 7 RE. S 3 6 10 /.616 l�� MERITECH, INC. MentechSample ID#: Of,1% I� P Bloassay Sample Chain of Custody 642 Tamco Rd, Reidsville NC 27320 Phone. 1-336-342-4748 Fax: 1-336,342-1522 Toxicity Supervisor email. mike.reed@meritech-labs.com Web Site: www.meritech-labs.com CLIENT INFORMATION Client L!jt..Jt.7 U !�[ (1,n -}a <r7 POM Contact Person {� is C YJ NPDES#'. NC CX.' ` .,V? Address: L� C&:g ti\4,- Phoner2 ff l -1 ZM13 City: �� Ocs.t� Pipe #.* tel, t County: L ("LL, -250V / State: 1�L- zip: ? -1:5 2 � SAMPLE INFORMATION Sample Site F4 i_,-&,- Sample Type: ❑ Grab ®-Composite # of containers: Sampling Time: Start Date: kL2'` ! } "Z Start Time: .02`30 AM PM End Date:(tom t I -I _ End Time: °�y � PM '** Triple rinse sample container with sample before Filing. Completely till the sample container with no airspace. Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory' Collector's Name: Print. J int' \I+C ��`t�� �3 Signature: �fQC� Test Required: © Chronic V days) ❑ Acute (24-48 hours) jwc: U % Comments: TOXICITY TEST INFORMATION Test Organism: ❑2 Cerlodaphnia dubia (water flea) Pimephales promelas (fathead minnow) ❑ Mysldopsls bahla (shrimp) Test Concentrations (if multiple dilutions): ;p_ �% SHIPPING INFORMATION Relinquished by �`Date: 117-7 1 11 Received j / -' Date: /A2 7// % Relinquished b /— Date: Received by: Relinquished by: Received by. Relinquished by: Received by: Date: Date: Date: Date: Date: Sample Temperature CC): Time: �5 ~ AM PM Time• /I3 S<: 5AP PM Time: 1Yy s AM Time: AM PM Time, AM PM Time: AM PM Time: AM PM Time: AM PM Method of Shipment:❑ UPS ❑ Fed EX ❑ Meritech Pick-up E] Delivered ❑ Other ** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED ** jj SAMPLE RECEIVING (Laboratory Use Only) Relinquished by: lh 21C 4r !) �n ls- Received by i1 \tiAk Date: j 1 Time: M5 5 AM� Sample Temperatures CC): % _ 4, I r 11-1-1 Sample Condition: WHITE = Laboratory copy YELLOW = Client copy Title: Benson File: BensonGrowth Transform: NO TRANSFORMATION Shapiro - Wilk's Test for Normality ---------------------------------------------------------------------------- D = 0.1089 W = 0.9783 Critical W = 0.8840 (alpha = 0.01 , N = 24) W = 0.9160 (alpha = 0.05 , N = 24) Data PASS normality test (alpha = 0.01). Continue analysis. Title: Benson File: BensonGrowth Transform: NO TRANSFORMATION -- Bartlett's Test for Homogeneity of Variance ---------------------------------------------------------------------------- Calculated B1 statistic = 5.0298 (p -value = 0.4123) Data PASS B1 homogeneity test at 0.01 level. Continue analysis. Critical B = 15.0863 (alpha = 0.01, df = 5) = 11.0705 (alpha = 0.05, df = 5) Title: Benson File: BensonGrowth Transform: NO TRANSFORMATION ANOVA Table ---------------------------------------------------------------------------- SOURCE DF SS MS F ---------------------------------------------------------------------------- Between 5 0.0284 0.0057 0.9398 Within (Error) 18 0.1089 0.0061 -----------------------------------------7---------------------------------- Total 23 0.1374 ---------------------------------------------------------------------------- (p-value = 0.4791) Critical F = 4.2479 (alpha = 0.01, df = 5,1.8) = 2.7729 (alpha = 0.05, df = 5,18) Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05) N Title: File: Benson BensonGrowth Transform: NO TRANSFORMATION Dunnett's Test - TABLE 1 OF 2 Ho:Control<Treatment -CALCULATED ------------------------------------------ - - - TRANSFORMED MEAN IN SIG GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0.05 ---------------------- 1_- -- Control ----- 0.8908 0.8908 2 22.50 0.9258 0.9258 -0.6363 3 45.0 0.9925 0.9925 -1.8498 ,4 75 0.9598 0.9598 -1.2544 5 90 0.9220 0.9220 -0.5681 6 100 0.9733 0.9733 - -1.4999 Dunnett --- critical value= 2.4100 (1 Tailed, alpha =0.05; df = 5,18) Title: Benson File: BensonGrowth Transform: NO TRANSFORMATION 1 ------Dunnett's_Test---y---TABLE-2-OF-2-------------Ho_Control<Treatment-_-- NUM OF MIN SIG DIFF % OF DIFFERENCE GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL --- 1 --- Control 4 2 22.50 4 0.1326 14.9 -0.0350 3 45.0 4 0.1326 14.9 -0.1017 4 75 4 0.1326 14.9 -0.0690 5 90 4 0.1326 14.9 -0.0313 6 --------------------------------------------------------------------------- 100 4 0.1326 1.4.9 -0.0825 1 1 Title: Benson NO TRANSFORMATION File: BensonSury Transform: Shapiro - Wilk's Test for Normality ---------------------------------------------------------------------------- D = 0.0325 W = 0.8227 Critical W = 0.8840 (alpha = 0.01 , N = 24) W = 0.9160 (alpha = 0.05 , N = 24) Data FAIL normality test (alpha = 0.01). Try another transformation. Warning - The first three homogeneity tests are sensitive to non -normality and should not be performed with this data as is. t Title: Benson File: BensonSury Transform: NO TRANSFORMATION I Hartley's Test for Homogeneity of Variance Bartlett's Test for Homogeneity of Variance ---------------------------------------------------------------------------- i These two tests can not be performed because at least one group has zero variance. I Data FAIL to meet homogeneity of variance assumption. Additional transformations are useless. --------------------------------------------------------------------- Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 12/07/16 Facility: TOWN OF BENSON WWTP NPDES#: NCO020389 Pipe#: 001 County: JOHNSTON Laborat er ormin T t: MERITECH LABS, INC. �� Q� Comments: e X i Z-0—P�-� - S1 ature Ot Laboratory Supervisor * PASSED: 0.37. Reduction Work Order; Environmental Sciences Branch MAIL ORIGINAL TO: Div. of Water Quality N.C. DENR 1621 Mail Service Center North Carolina Ceriodaphnia Raleigh, North Carolina 27699-1621 Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results Calculated t = CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 . Reduction = 0.37 # Young Produced 26 27 19 21 22 20 21 24 18 23 26 24 ' Mortality Avg.Reprod. 0.00 22.58 Adult (L)ive (D)ead L L L L L L L L L L L L Control Control 8.33 22.50 Effluent .: 90% Treatment 2 Treatment 2 TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 ZO 11 12 Control CV 12.8700 PASS FAIL # Young Produced 23 13 25 25 27 25 28 24 25 26 23 6 . control orgs pCheck producing 3rd Adult (L)ive (D)ead L D L L L L L L L L L Lbrood100;90,e 1st sample 1st sample 2nd sample Complete This For Either Test PH Test Start Date: 11/30/16 Control 7.64 8.16 8.06 8.14 [7.64 .01 8.00 Collection (Start) Date Treatment 2 7.42 8.09 7.56 708 8.05 Sample T 11/28/16 Sample 2: 11/30j16 P Type/Duration 2nd s s 1st P/F s Grab Comp. Duration D t e t e t e T S S a n a n a n Sample 1 X 24.0 hrs L A A r d r d r d U M M t t t Sample 2 X 24.0 hrs T P P 1st sample 1st sample 2nd sample D.O. Hardness(mg/1) 46 Control 8 . 15 7.95 8 . 11 8 . 00 7.72 7.78 '•"'••• ......••• rreatment 2 8.10 7.96 8,25 7.86 8.39 7,85 Spec, Cond.(pinhos) 170 301 316 Chlorine(mg/1) <0.1 <0.1 LC50/Acute Toxicity Test Sample temp. at receipt(°C) 1.6 0.8 (Mortality expressed as ., combining replicates) 16 Concentration Note: PleaseComplete This o ¢ Section Also LC50 = g 950 Con i ence Limits a a Mortality start/end start/end Method of Determination Control Moving Average Probit Spearman Karber Other — High Conc. PH D.O. Organism Tested: Ceriodaphnia dubia Duration(hrs): Copied from DWQ form AT -1 (3/87) rev. 11/95 (DUBIA ver. 4.41) Meritech, Inc. Client: �1 Mini Chronic Pass/F it Test: Ceriodaphrria dubs j Pipe #: QQ1 County: Date Start: / 1 r� NPDES #: NC D Date / Time of Culture Transfer: �i Time Start- �:�� Dilution Water: Lake Brandt Dafe /Time Neonates born: _ I�`-i phn�3/1st Renewal Date: G)a a - Test Organism Source: Tray* 1— Age of lyRonates at Test Start:o�• ,Shours 2nd Renewal Date: r '- Stirred /Aerated for D.O.: Y /lw) Randomized (Y)/ N r`„i+„tea r.,,., nt. n - �� ta, 6 Dav #2 1 7 a n c # Young ProducedPz- 7 0 - 0 0 0 0 0 o 0 y 0 I 0 11 0 12 0 Adults Live/ DeadL 4 5 LL L (__ l: L. L L L 0 # Young Produced Adults Live / Dead Total Produced 1L -!n 1 2_ 3 4 b 6 7 8 9 10 11 12 /a 14/.,1311,15/2,19 /-- (P/ I ,/, Ibl1 �-I L_I ` IL 6 7 8 9 Percent of Control producing third brood: /01) % Test Sam le Organism Reproduction 7 8 Effluent% .�, 10 11 12 0 0 Pay #2 1 2 3 4 5 # Young Produced 0 0 0 0 0 Adults Live / Dead L. L Lr L L_ DM #8 1 2 3 4 5 *Young Produced �/� S 5�� �1 Adults Live / Dead L 6 L, L_ L Day #7 1 2 3 4 5 # Young Produced fj � f �3 I� _Adults Live/ Dead L f Z) I L_ IL- I s . Total Produced ,Comments: 6 7 8 9 10 11 12 0 0 0 0 0 0 0 (_ (___ 6 7 9 10 11 J8 !1^2 6 7 8 9 10 11 12 Incubator#: C Date End: 1 :;�L _7_96 Time End:'r�} Time - Sample 1: - —R --16 Sample 2: Sample Information 100% pH G / C? Duration Sample 1 �- C- ,7 hours Sample 2 � 2 % hours Batch #Q;6) Transferred by. Fed by � y % i Sample 1 Sample 2 oCIOV / Transfer Daya I Day �V 5 .��t�.` ��' a Day 1 Hardness .c`Day 2 ' L Spec. Cond.Day 3' (umhos/cm) 17 30 1 3 I Chlorine +�'`d '' Day 4 1_V/ (m9/L) `> �r_=;t^;%.:-'r; LC�� j Cv - j Day 5 4v-. ReceiptSampieIr"a �w Day 6 Temp (°C) ;.:ti��f`, �,r. ' ,s,T �, �• Terminated b4(� Yj pH 1st Sample 2nd Sample 2nd Sample Control fv'� «' Wa�rjna Eln,05 -"Vo Sample �'•G°f F•dnitla final final D.O. 1st Sample 2ndSample 2nd Sample Control (f i �� 7 �5+ F, 11 77c� Samples �� 71 ° i rFSJ� ml la final n1Ua fin i Ual flnat Temp. 1st Sample 2nd Sample 2nd Sample Control A% -(�./ ��, I a •a t f' Sample nil MERITECH, INC. MentechSample ID#:• 11 i� J� Bioassay Sample Chain of Custody - 642 Tamco Rd, Reidsville NC 27320 Phone: 1-336-342-4748 Fax., 1-336-342-1622 Toxicity Supervisor email: mike.reed0mieritech-labs.com 'Web Site: www.meritech4abs.com (� }}CLIENT INFORMATION Client ? • ,-,v.1 C0'�n ��C,vV�y it-' PO#: Contact Person: c t1` It `L_ NPDESP NCOQu T&T Address: City' County: Phone:lei'f-1�Q "1 13 13 Pipe M. U O Zip: Ly SAMPLE INFORMATION ` Sample Site: Sample Type: ❑ Grab liComposite # of containers: Z Sampling Time: Start Date: fj �Z� ` Z t o Start Time: OV 3 ojR PM End Date. i (l --r l L,- End Time: A3 61 PM *'* Triple rinse sample container with sample before filling. Completely fill the sample sontalner with no airspace. Pack the sample cooler completely in ice. The sample must be < 6.01C upon receipt at the laboratory*"* Collector's Name: Print: 'C� Y � ��i,��� Signature: Test Required: ❑ Chronic (7 days) ❑ Acute (24-48 hours) IWC: F a % Comments: TOXICITY TEST INFORMATION Test Organism: arerlodaphnia dubfa (water flea) 5g Pimephales promelas (fathead minnow) ❑ Mysidopsis Bahia (shnmp) Test Concentrations (ff multiple dilutions): Relinquished by f� ff SHIPPING INFORMATION Time: R � PM Relinquished by. Received by -[ Date- R Date: - %� Time. Time: )l'� ,� d Jivl PM PM Relinquished by Date: Time: -1 AM PM Received by: Date Time: AM AM PM Relinquished by Date: Time: AM PM Received by: Date: Time: AM PM Relinquished by. Date. Time: AM PM Received by: Date: : • Time: AM PM Sample Temperature (°C): Method of Shipment:❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑ Delivered, ❑ Other "Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED •* (, SAMPLE RECEIVING (Laboratory Use Only) Relinquished by: irl Received by Date: f �� l' Time: . 3 5^ AM Sample Temperatures(°C}: `� A% ! Sample Condition: N -t' mmmmmmmmw WHITE = Laboratory copy YELLOW = Client copy MERITECH, INC, MentechSample ID# � Bioassay Sample Chain of Custody 642 Tamco Rd, Reidsville NC 27320 Phone: 1-336-342-4748 Fax: 1-336-342-1522 Toxicity Supervisor email: mike.reed(Mmeritech-labs.com Web Site, www.meritech-labs.com CLIENT INFORMATION Client ,Q C> POP Contact Person: 9-t,3 1 �1 �_'1 3 7 NPDES#. NC C7�d g Address: U �� t ��} `�. L `r1 ��y C� �c,- -Phone. �`rffl � �3 City: ,,'3 %I- L7:)� 5 -Pipe #•��— • . County 0 \2,& CC7,0,J State: �_ Zip: ' J SAMPLE -INFORMATION Sample S16:F-f U ,o} Sample Type: ❑ Grab Q Composite # of containers: Sampling Time: Start Date: ti � r��Cz Start Time: (3!:l End Date: 4f Z \ (cam End Time: GS`; 3C) f 3pk' Pjdl *`* Triple rinse sample container with sample before filling. Completely fill the sample Goritalner with no air space. Pack the sample cooler completely in ice. The sample must be < 6.00 upon receipt at the laboratory*** Collector's Name: Print Signature: - .z - TOXICITY TEST INFORMATION Test Required: Chronic (7 days) Test Organism: ❑ Ceriodaphnia dubia (Watei flea) ❑ Acute (24-48 hours) ©. Pimephales promelas (fathead minnow) ❑ Mysidopsis bahia (shrlthp) IWC: ZIC2 % Test Concentrations (if multiple dilutions):_ Comments: SHIPPING INFORMATION Relinquished by: - Date: Received by 1. Date: - Relinquished b� . : Date: Received by.(—/Date: Relinquished by: Received by - Relinquished by: Received by. Date: Date, Date. Date. Sample Temperature CC): Method of Shipment❑ UPS ❑ Fed EX ❑ Meritech Pick-up Time:+ 0PM t Time: .' AM PM Time: - AM PM Time: AM PM Time: AM PM Time: AM PM Time: AM PM Time. -AM PM ❑ Delivered ❑ Other " Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED" SAMPLE RECEIVING (Laboratory Use Only) - Relinquished by: tl}�{i, • f. t� Received y: Date: rl ? [ Time: - AM PM Sample Temperatures ( ):� i� / 1 Sample Condition: L WHITE = Laboratory copy YELLOW = Client copy Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 12/07/16 Facility: TOWN OF BENSON WWTP NPDES#: NCO020389 Pipe#: 001 County:JOHNSTON Laboratory Performing Test: MERITECH LABS, INC. Reduction: 0.371 CONTROL 90P. -.Effluent # Replicates 12 12 Female Live 12 11 Adult Male 0 0 Adult Dead 0 1 Adult Mortality 0.001 8.331 # Neonates 271 270 Mean # Neonates 22.583 22.500 Standard Deviation 2.906 6.417 Coefficient of Variation 12.870% E Fisher's Exact Test A = 12 B = 12 a = 12 b = 11 a/A = 1.00 b/B = 0.92 Success is: survival Critical b value = 8 11 > 8 The test concludes that the proportion of survival is not significantly different for the control and the effluent groups. Test Passes! SHAPIRO-WILIC'S TEST FOR NORMAL DISTRIBUTION OF DATA ORDERED OBSERVATIONS i Group Neonates Centered i Group Neonates Centered 1 E 6 -16.5000 13 C 24 1.4167 2 E 13 -9.5000 14 E 24 1.5000 3 C 18 -4.5833 15 E 25 2.5000 4 C 19 -3.5833 16 E 25 2.5000 5 C 20 -2.5833 17 E 25 2.5000 6 C 21 -1.5833 18 E 25 2.5000 7 C 21 --1.5833 19 C 26 3.4167 8 C 22 -0.5833 20 C 26 3.4167 9 C 23 0.4167 21 E 26 3.5000 10 E 23 0.5000 22 C 27 4.4167 11 E 23 0.5000 23 E 27 4.5000 12 C 24 1.4167 24 E 28 5.5000 11 Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date.12/8/2016 Facility- Town of Benson NPDES # NC00 20389 Pipe*: 001 County: Johnston Laborat : Meritec , Inc Comments XZ�.9Z Signature of Operator in Re sible C X 9 ��y��.'` g Signature of Laboratory Supervisor MARL ORIGINAL TO: Environmental Sciences Branch Division of Water Quality NC DENR 1621 Mail Service Center Raleigh, NC 27699-1621 Test Initiation Date/Time 11/2912016 2.10 PM 1211/2016 Avg WVSurv. Control 0.814 Test Organisms % Eff. Repl. 1 2 3 4 32 r Cultured In -House Control Surviving # 10 10 10 10 % Survival 100.0 Ev: Outside Supplier Original # 10 10 10 10 Wtloriginal (mg) 0 822 0.890 0 780 0 763 Avg Wt (mg) 7_05-1-T-1 Hatch Date. 11/28/16 22.5 Surviving # 7 10 10 10 % Survival 92.5 Hatch Time. 3.00 pm CT Original # 10 10 10 10 Wt/original (mg) 0.573 0 822 0.793 0.885 Avg Wt (mg) 0.768 45 Surviving # 10 10 9 9 % Survivall 95.0 Original # 10 10 10 10 Wt/original (mg) 0 749 0.733 0.721 0 517 Avg Wt (mg) 0.680 F-7-5-1 Surviving # 9 10 9 10 % Survival 95.0 Original # 10 10 10 10 Wt/original (mg) 0.857 0.970 0.849 1 0.973 Avg Wt (mg) 0 912 90 Surviving # 9 10 10 10 % Survival 97.5 Original # 10 10 1 10 10 Wt/original (mg) 0.929 0.922 0.882 0 788 Avg Wt (mg) 0,880 100 Surviving # 10 10 8 10 % Survival 95.0 Original # 10 10 10 10 Wtloriginal (mg) 0.878 0 875 0 679 0.940 Avg Wt (mg) 0.843 Water Quality Data Day Control 0 1 2 3 4 5 6 pH (SU) Init/Fin 8.10 1 7961817 1 7.72 8.12 1 771 8.17 / 792 8.25 / 7.68 8.00 / 7.97 8 18 / 7.65 DO (mg/L) Init/Fin 7.69 1 688 7.65 1 5.97 7.72 / 656 8.01 / 7.33 811 1 658 801 17 52 8.00 / 6.10 Temp (C) Init/Fin 269 / 24.6 25.5 / 24.8 257 / 259 242 1 25.4 243 / 24.5 24.6 / 247 124.1 / 25.9 High Concentration 0 1 2 3 4 5' 6 pH (SU) IniUFin 7.18 17 71 737 1 7.35 1742 / 7.47 17.46 / 7.791757 / 7.47 7.34 t 7.64 17.40 / 7.49 8.49 / 718 810 / 6041795 / 6.58 7.93 / 7561798 / 645 18.37 / 7.2$ 8.11 / 6.14 DO (mg/L) Init/Fin 25.9 / 25.7 248 / 25.9 24.5 1 24.1 124.4 / 242 125.9 / 24.5 1249 1 24.4 124.7 / 244 Temp (C) [nit/Fin Sample Collection Start Date Grab Composite (Duration) Hardness (mg/L) Alkalinity (mg/L) Conductivity (umhos/cm) Chlorine(mg/L) Temp. at Receipt (°C) 1 7 3 11/28/2016 11/30/2016 1211/2016 Normal n F1 240 24.0 24.0 30 32 32 24 37 29 323 352 1 362 <0A <0.1 <0.1 Dilution H2O Batch # F201 1097 Hardness (mg/L) 40 Alkalinity (mg/L) 52 Conductivity (umhos/cm) 202 Overall Result ChV >100 Stats Survival Growth Normal n F1 Hom. Var. rl tri NOEC 100 100 LOEC >100 >100 ChV >100 >100 Method Steel's Dunnett's Overall Result ChV >100 Stats Survival Growth Cone. Critical Calculated Critical Calculated 22.5 10 16 241 0.0455 45 10 14 241 0.1337 75 10 14 241 -0.0985 90 10 16 10 16 2.41 -00665 2.41 -00292 100 Client: Benson AId► 13rAL=C7",fir ljvc�, ENVIROIVMEIVTAL LABORATORIES A Division of Water Technology and Controls, Ir,c Chemical and Physical Determinations NPDES9: 0020389 Start Date: 11/29/16 End Date: 12/6/16 Day Concentration: control 0-1 1-2 2-3 34 4-5 5-6 T6-7 6-7 Remarks Soft Synthetic reshwater Batch # : j0 f �. q 7-7 "Y Final pH: Initial Final D.O.: Initial Final �� %� t y -7 l 1 �' 7-7a (� �o� -7-7 7 7. a t (S�' i t .. 2 SSi �S«� Temp.: Initial ..Gq,2,9-q,-3 g Final Q v I _7�% Final �• �� �-� �9+) Temp.: Initial Lf k _ Conductivity: Initial(��Y7u !Lf, -7 a Final -4'22i DLr, ,�i . `� Lf. Final 07 c`Z a /-6,j r� 1 R,5;F _a,20 �' °I "2 Residual Chlorine: ? j was i I Z-0r-�f_4 • L 0 Hardness: ti q wt l 4fa i 14611 Z-0, I Z,/)t. ` Alkalinity: T)av Concentration: 22.50% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks II: Initial '1 �. q 7-7 "Y Final Fugal D.O.: Initial t `7 j -7, (0 7- I F5- . �%� Final 7 g Final t�'� �� lfG _7�% Temp.: Initial Final �• �� �-� �9+) Temp.: Initial �. 111 % k _ InitialFinalResidual !Lf, -7 a Final LLIConductivity: DLr, ,�i . `� Lf. Chlorine: dj /-6,j 40.t R,5;F _a,20 - az nny 07 Concentration: 45.0% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH: Initial 7, a '1 �. q 7-7 "Y Final `_-Y Q .GD D.O.: Initial . �%� `I 7 g Final t�'� �� lfG _7�% �'��/ �• �� �-� �9+) Temp.: Initial �. 111 % k _ �� !Lf, -7 a Final I-,) DLr, ,�i . `� Lf. Conductivity: Initial G R,5;F _a,20 - az Final 2 72- '7 Residual Chlorine. 11�,- d'.) wt l 4fa i 14611 Z-0, I Z,/)t. ` B~C q ENVIF;C/N MENTAL LA80 RAT012[ES orvt:ron or• wa+es� ra�nn�ravr.-.•,> c �e.br9. r.+a. Chemical and Physical Determinations Client: Benson NPDES#: 0020389 StartDate: 11/29/16 End Date: 12/6/16 Dav Page 2 of 2 Concentration: 75% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH• Initial 7, L-1:5 %. "7. 60 < -4 T q 5 Final 7• qP) 7 - 'T-5 eqiq L) D.O.: Initial ? 1 , E 3 7-4/--/ 0 • % %1 to Fina! 7� �] ✓� �1'� �7 �_ S �� U" �a t �� Temp. Initial 2-A -: 21-� a LO Final C6,1 a�� Ag 2,�'(�� L(, Conductivity: Initial '306 `a FR --3 a IT 3041 Fina! -31-3 3U 1 `�j wa 3'3 19-q 7 �US Residual Chlorine: -- D, i ZQ_ J G(�, j SCJ• f GG, ,), ri Day Concentration: 90% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH: Initial 7,) `7 tjE 7-6a < -4 T q 5 Final �, f%a U - �, �l eqiq L) D.O.: Initial ? 1 , E 7 C d1 ,�./7 `/tib C� oZ r �• Final (n. }y 6.5C. S / /U� `l. �� 1 r'J , ILI Temp.: Initial7�- 2-A -: 21-� a LO Final C6,1 a�� Ag 2,�'(�� L(, Conductivity: Initial -5 j y 3 (oO v313 Final 3051 312 369 31S 77% 19-q 7 �US Residual Chlorine:) 4 V , Y LD, G�}. % ��, ( G(' �) L (j, j ri Day Concentration: 100% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks pH: Initial 7,) 7--12 7.t& < -4 - -3 Final �N -7, r tt % - "7., D.O.: Initial , E 1. �_ �� q7 -3 ,�./7 `/tib � , Final (n. }y 6.5C. S / /U� `l. �� 1 r'J , ILI Temp.: Initial 2-A -: 21-� a LO Final �,�� a�� -2_`1'I 9LO'/'xq-6 Conductivity: Initial 3 j y 376 '-5 R Final '3 1 ,15 r 3(�f 373 �US Residual Chloiine: 4 V , Y ZC7 , j z -o . r ZO. 1 ZO. j Z -o .) /-0,/ Hardness: 32 F Alkalinity: MERITECH, INC. Mortality Data: Chronic Fathead Test Client: Benson NPDES#: 0020389 StartDate: 11/29M6 End Date: 1216/16 Concentration 75% Rep A Rep B Rep C Rep D Tuesday 0 Wednesday Thursday v Friday Saturday Sunday Monday V Tuesday 0 J 0 0 Concentration 90% 90% A Rep B Rep C Rep D Tuesday 0 Wednesday Thursday URep ,�. ' v l Friday Saturday Sunday Monday —�- Tuesday v 0 �! 0 0- Concentration 100% Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday V � V Rep A 0 y �� Rep B 0 v r�. Rep C 0 Rep D 0 A' P ERI Fw , 1 1GYI C. ENVIRONMENTAL LABORATORIES • �� - A Division of Wain Technology and Contra/5, /nc. FISH LARVAL SURVIVAL/GROWTH DATA SHEET CHRONIC FATHEAD MINNOW Facility: Benson NPDES #: 0020389 Date of Test: 11/29/16 Initial weights taken on: by: D Final weights taken on: " �'� by: LV Page: 1 of 2 outfall: Organism: Plmej2hales pnomelas CONC REP PAN WT. (mg) PAN + ORG. WT. (mg) WT. OF ORG. (mg) # ORG. IWTJORG. MEAN (mg) SURV % A .`I Icgr bo to 0- 9-1)Q .r 1. " 0 , 9;)1 7 �0 L00— c D D lR oR? f 7�P� A i 3r� i 1�1 � 3 5� 6.5 7 2� B 17 �S�aS g to 22.50% c l.3 ` 3 �� J�1� L, 7:- D A REP- a 3 R a q. 7121� LIq I -7q q B g .0 3� o 45.0% tEl R3a.33 2:Aa �, �•� 1Z/ y Rlq-o a_�l 0D V Facility: Benson NPDES #: 0020389 MEi TCS 1g ENVIRONMENTAL LABORATORIES A DIVIsfon orvvater %chnology and Controls mc- FISH nc FISH LARVAL SURVIVALIGROWTH DATA SHEET CHRONIC FATHEAD MINNOW Date of Test: 11/29/16 Initial weights taken on: q-30-16 by: C Final weights taken on: .1az-7-11, by: LV Page: 2 of 2 Outfall: Organism: Pimephales promelas CONC REP PAN WT. (mg) PAN + ORG. WT. (mg) WT. OF ORG. (mg) # ORG. MEAN WTJORG. (mg) SURV % A 3t�1��`� �a3.�6 R., -/; q G,b,57 l o 75/o B R1-/1. -75 g614 u q--70-10 o: q. 160 c �,oq I] U f �� �lC� `l/(� q-7) Dn �� �� A I y 1 o LI -10 B 3� q,�o 6oBaa 90% c RV RS 31 cL - oL ry� Zod D ?3766 Rq, aS7 7, YY !U A 5 S .7 'Rt'7 %a o 1oa/o B X37 aa F'4�,7-7 �- 75 10 0,—F /DU c 03G-0 E 6. 7q F -KO D 1233-7Q MERITECH, INC. MentechSample ID* l� iia l� u3 Bioassay Sample Chain of Custody 642 Tamco Rd, Reidsville NC 27320 Phone: 1-336-342-4748 Fax: 1-336-342-1522 Toxicity Supervisor email: mike reed(Mmeritech-labs.com Web Site: wwwa6ritech-labs.com CLIENT INFORMATION Client 1.-,,-j PO#1 Contact Person: C \ NPDES#.- NC Address �Z� I��VOv`�-� h �t ^C Phony City: YcvS Pipe #- et -.,i County: � )r xt Ao4'! State: G �A' Z- kA SAMPLE INFORMATION Sample Site: rr_ q' \'-) Sample Type: []Grab 5 Composite # of containers, Sampling rime: StartDate: t''Z !>; l t, a StartTime:Q '3 PM ° '[ PM End Date: �) 2 t t _ End Time: U K � C/ 1 Imo/ *`* Triple rinse sample container with sample before filling. Completely frit the sample container with no air space. Pack the sample cooler completely in ice. The sample must be < 6-0°C upon receipt at the laboratory' Name; Pnnt--ay-- '.4 Y_ `�" \ Uf�� f, 'r Signature: �r TOXICITY TEST INFORMATION Test Required: © Chronic (7 days) Test Organism: ❑ Cerlodaphrila dubia (wate11 f flea) ❑ Acute (24-48 hours) Qd PFmephales promelas (fathead minnow) ❑ Mysidopsis bahla (shrimp) iWC: U % Test Concentrations (if multiple dilutions): comments: SHIPPING INFORMATION Relinquished b: q y ` '`� Date: „f2, n'14 Time: Q U �PM � _ �— Date: oZ , ,R (� Time: ' Received b ���--� PM Relinquished by Date: /2 �� l/� Time: j350 AM (M Date: Time: AM PM Received by: Method of Shipment❑ UPS ❑ Fed EX ❑ Meritech Pick-up L] Delivered . ❑ Other " Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "* SAMPLE RECEIVING (Laboratory Use Only) Relinquished by: � i - Received by: a Dale: � I � J 11. Time: 1350 AM PM Sample Temperatures (°C). A• 5 1 0.5 I 05 l Sample Condition: EMI po- WHITE = Laboratory copy YELLOW = Client copy Date: Time: AM PM Relinquished by: Date: _ Time: AM PM Received by: Date: Time: AM PM Relinquished by: Date: -Time. AM PM Received by: Sample Temperature CC): Method of Shipment❑ UPS ❑ Fed EX ❑ Meritech Pick-up L] Delivered . ❑ Other " Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED "* SAMPLE RECEIVING (Laboratory Use Only) Relinquished by: � i - Received by: a Dale: � I � J 11. Time: 1350 AM PM Sample Temperatures (°C). A• 5 1 0.5 I 05 l Sample Condition: EMI po- WHITE = Laboratory copy YELLOW = Client copy Title: Benson File: BenGrowth Transform: NO TRANSFORMATION Shapiro - Wilk's Test for Normality ---------------------------------------------------------------------------- D = 0.1659 W = 0.8879 Critical W = 0.8840 (alpha = 0.01 N = 24) W = 0.9160 (alpha = 0.05 N = 24) Data PASS normality test (alpha = 0.01). Continue analysis. .1 e Title; Benson File: BenGrowth Transform: NO TRANSFORMATION Bartlett's Test for Homogeneity of Variance ---------------------------------------------------------------------------- Calculated B1 statistic = 3.2777 (p -value = 0.6573) Data PASS B1 homogeneity test at 0.01 level. Continue analysis. ------------------------------------------------------------------------- Critical B = 15.0863 (alpha = 0.01, df = 5) = 11.0705 (alpha = 0.05, df = 5) Title: Benson File: BenGrowth Transform: NO TRANSFORMATION .ANOVA Table ---------------------------------------------------------------------------- SOURCE DF SS MS -------------------------------- ---Between----------- 5 0.1396 0.0279 3.0288 Within (Error) 18 0.1659 0.0092 ---Total--------------23---------------0-3055-------------------------------- (p -value = 0.0372) Critical F = 4.2479 (alpha = 0.01, df = 5,18) = 2.7729 (alpha = 0.05, df = 5,18) Since F > Critical F REJECT Ho: All equal (alpha = 0.05) I i " 2 Title: Benson BenGrowth Transform: NO TRANSFORMATION File: Dunnett's Test - TABLE 1 OF 2 -Ho_Control<Treatment --------------------------------------- - -- -- - - - ----- TRANSFORMED MEAN CALCULATED IN SIG GROUP IDENTIFICATION MEAN --ORIGINAL UNITS T STAT 0.05 -------------------------- 1 Control -------- 0.8138 0.8138 0.7683 0.6702 2 22.50 45.0 0.7683 0.6800 0.6800 1.9700 3 75 0.9123 0.9123 -1.4508 4 90 0.8803 0.8803 -0.9795 5 6 100 0.8430 0.8430 -0.4308 Dunnett -critical -value = 2.4100 - (1 Tailed, alpha = 0.051 df = 5,18) Title: Benson NO TRANSFORMATION File: BenGrowth Transform: Dunnett's Test - TABLE 2 OF 2Ho:Control<Treatment --------------------------- ------------ ------------------------------------ NUM OF MIN SIG DIFF o OF DIFFERENCE GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL 1 Control 4 22.50 4 0.1636 20.1 0.0455 3 45.0 4 0.1636 20.1 0.1337 4 75 4 0.1636 20.1 -0.0985 5 90 4 0.1636 20.1 -0.0665 6 100 4 0.1636 20.1 -0.0292 Title: Benson NO TRANSFORMATION File: BenSury Transform: Shapiro - Wilk's Test for Normality D = 0.1250 W = 0.8305 Critical W = 0.8840 (alpha = 0.01 , N = 24) W = 0.9160 (alpha = 0.05 , N = 24) Data FAIL normality test (alpha = 0.01). Try another transformation. Warning - The first three homogeneity tests are sensitive to non -normality and should not be performed with this data as is. Title: Benson Transform: NO TRANSFORMATION File: BenSury Hartley's Test for Homogeneity of Variance Bartlett's Test for Homogeneity of Variance _^_______ These two tests can not be performed because at least one group has zero variance. Data FAIL to meet homogeneity of variance assumption. Additional transformations are useless. Title: Benson File: BenSury Transform: NO TRANSFORMATION Steel's Many -One Rank Test - Ho: Control<Treatment ----------------------------------------------------------------------------- MEAN IN RANK CRIT. SIG GROUP IDENTIFICATION ORIGINAL UNITS SUM VALUE DF 0.05 1 Control 1.0000 2 22.50 0.9250 16.00 10.00 4.00 3 45.0 0.9500 14.00 10.00 4.00 4 75 0.9500 14.00 10.00 4.00 5 90 0.9750 16.00 10.00 4.00 6 100 0.9500 16.00 10.00 4.00 ---------------------------------------_--------------------------------------- critical values are 1 tailed ( k = 5 )