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HomeMy WebLinkAboutNC0004961_Assessment of Balanced & Indigenous Populations_20090801ASSESSMENT OF BALANCED AND INDIGENOUS POPULATIONS IN MOUNTAIN ISLAND LAKE NEAR RIVERBEND STEAM STATION NPDES Permit No. NC0004961 Principal Investigators: Michael A. Abney John E. Derwort Keith A. Finley DUKE ENERGY Corporate EHS Services McGuire Environmental Center 13339 Hagers Ferry Road Huntersville, NC 28078 August 2009 ACKNOWLEDGMENTS The authors wish to express their gratitude to a number of individuals who made significant contributions to this report. First, we are much indebted to the EHS Scientific Services field staff in carrying out a complex, multiple -discipline sampling effort that provides the underpinning of this report. We would like to thank Glen Long for support in water quality and sediment sample collections. Mark Auten, Kim Baker, Bob Doby, James Hall, Bryan Kalb, Glenn Long, and Todd Lynn were vital contributors in completing fisheries collections and sample processing. James Hall, Aileen Lockhart, Shannon McCorkle, and Jan Williams contributed in macroinvertebrate sampling, sorting and taxonomic processing. We would also like to thank multiple reviewers; including Penny Franklin, Duane Harrell, Ron Lewis, and John Velte. The insightful commentary and suggestions from these individuals and also between co-authors have benefited the report in myriad ways. lu TABLE OF CONTENTS EXECUTIVESUMMARY....................................................................................................v LISTOF TABLES............................................................................................................... vii LIST OF FIGURES............................................................................................................ viii CHAPTER1- INTRODUCTION........................................................................................1-1 REGULATORY CONSIDERATIONS..........................................................................1-2 CHAPTER 2- STATION OPERATION............................................................................ 2-1 METHODS...................................................................................................................... 2-1 RESULTS AND DISCUSSION..................................................................................... 2-1 StationOperation......................................................................................................... 2-1 ThermalCompliance....................................................................................................2-2 Compliance with EPA Nomograph -Related Thermal Limits......................................2-2 CHAPTER 3- WATER AND SEDIMENT CHEMISTRY ............................................... 3-1 MATERIALSAND METHODS.................................................................................... 3-1 Collection of Field Parameters, Water, and Sediment Chemistry Samples ................. 3-1 AnalyticalMethods...................................................................................................... 3-2 RESULTS AND DISCUSSION..................................................................................... 3-3 WaterQuality............................................................................................................... 3-3 RSS Ash Basin Effluent Selenium and Arsenic Mass Loading Estimates .................. 3-8 Sediment Elemental Analyses...................................................................................... 3-9 CONCLUSIONS........................................................................................................... 3-10 CHAPTER 4- MACROINVERTEBRATES...................................................................... 4-1 MATERIALS AND METHODS.................................................................................... 4-1 RESULTS AND DISCUSSION..................................................................................... 4-1 Substrate....................................................................................................................... 4-1 Density......................................................................................................................... 4-2 TaxaAbundance.......................................................................................................... 4-2 CONCLUSIONS............................................................................................................. 4-3 CHAPTER5- FISH.............................................................................................................. 5-1 MATERIALS AND METHODS.................................................................................... 5-1 Winter and Summer Electrofishing Surveys................................................................ 5-1 Spring Electrofishing Surveys..................................................................................... 5-1 Fall Hydroacoustics and Purse Seine Surveys............................................................. 5-2 Trace Element Analyses.............................................................................................. 5-2 Balanced and Indigenous Assessment......................................................................... 5-2 RESULTS AND DISCUSSION..................................................................................... 5-3 Winter Electrofishing Surveys..................................................................................... 5-3 iii Summer Electrofishing Surveys.................................................................................. 5-3 Spring Electrofishing Surveys..................................................................................... 5-4 Fall Hydroacoustics and Purse Seine Surveys............................................................. 5-5 CONCLUSIONS............................................................................................................. 5-6 LITERATURE CITED.......................................................................................................L-1 APPENDIX TABLES....................................................................................................... A-1 IV EXECUTIVE SUMMARY Per agreement with the North Carolina Department of Environment and Natural Resources (NCDENR), annual monitoring of macroinvertebrates and fish was initiated in 1990 at selected locations in Mountain Island Lake to evaluate potential impacts of the Riverbend Steam Station (RSS) condenser cooling water discharge on balanced and indigenous populations in Mountain Island Lake. Water quality and sediment samples collected also provided information relative to the biological assessment. This report compares monitoring data collected from 2004 — 2008 and past studies. As in the past, during 2004 — 2008, RSS operated primarily as a peaking facility. Electric generation occurred on 81% of available days, with the entire station operating at an average of 48% of capacity over the period. Thermal limits specified in the RSS National Pollutant Discharge Elimination System (NPDES) permit were not exceeded. Average monthly condenser cooling water (CCW) discharge temperatures ranged from 4.9 °C (58.9 °F) in February 2005, to 34.7 °C (94.4 °F) in August 2007. These temperatures followed a regular seasonal pattern and were within the range of previously reported values. The discharge water temperature of RSS was continually monitored to ensure compliance and to assist in the investigation of potential impacts to downstream biota. Water quality in Mountain Island Lake during 2004 — 2008 was similar to previous years. Seasonal thermal stratification, and dissolved oxygen concentrations were representative of historical ranges. Lake -wide, concentrations of major nutrients, similar to historical levels, continued to result in Mountain Island Lake being classified as oligotrophic to mesotrophic during 2004 — 2008. Typically elevated concentrations of dissolved and suspended material, as well as nutrients entering the lake from the McDowell Creek drainage were slightly lower during 2004 — 2008. Mass loading of selenium and arsenic from the RSS ash basin outfall during 2004 — 2008 was similar to historical trends, except for reductions observed in arsenic loading over the 2007 — 2008 period. Mountain Island Lake concentrations of aqueous trace elements remained generally low, consistent with low levels of turbidity and suspended solids. March 2008 arsenic concentrations in fine surficial sediments were elevated downstream of RSS in the forebay of Mountain Island Hydroelectric Station (MINS) with respect to uplake v concentrations, with samples just downstream of the RSS ash basin effluent confluence yielding the highest concentrations. While the 2008 samples were likely influenced by a slight relocation of the sampling site due to difficulty in obtaining material other than coarse sand at the former location, MIHS forebay sediment sampling continues to indicate a slight, gradual increase in arsenic concentrations over recent years. Sediment concentrations of selenium were consistently low lake -wide. As a whole, 2004 — 2008 Mountain Island Lake water and sediment chemistry continue to indicate the waterbody remains capable of sustaining balanced and indigenous aquatic communities. Trace element concentrations measured in Mountain Island Lake common carp, sunfish, and largemouth bass since 1988 remain well below values of regulatory concern. As in previous years, annual samples of the macroinverterbrate community collected uplake, downlake, and adjacent to the RSS discharge area during 2004 — 2008 were comprised primarily of Oligochaeta, Diptera, and Corbicula. Comparison of macroinvertebrate densities and taxa abundance continues to indicate no apparent adverse effect of the heated discharge on the macroinvertebrate community in the vicinity of the discharge. Electrofishing samples have shown a persistent and stable fish community present in the littoral portions of Mountain Island Lake around RSS from 2004 to 2008. The assorted fish species typically found in Mountain Island Lake encompass a range of trophic groups including insectivores, omnivores, and piscivores that indicate a balanced and indigenous fish community. Forage fish population densities were comparable to those measured previously at Mountain Island Lake and to other Catawba River reservoirs of similar trophic status. The introduction of alewife, the inherent, temporal variability of clupeid populations, and the historical flux of Mountain Island Lake between oligotrophic and mesotrophic water quality classifications are all factors contributing to the unpredictable nature of forage fish densities. Based on the diversity and numbers of individuals in the Mountain Island Lake littoral fish community during winter, spring, and summer; the range of trophic groups represented; the lack of accumulation of selenium, arsenic, mercury, or zinc in fish flesh; and the regular availability of forage fish to limnetic predators; it is concluded that the operation of RSS has not impaired the protection and propagation of a balanced and indigenous fish community in Mountain Island Lake. vi LIST OF TABLES Table Title Page 1-1 Biological and water quality monitoring locations on Mountain Island Lake............ 1-5 2-1 RSS monthly units 4 through 7 (U4 — U7), monthly average (Av), and annual station coal-fired capacity factors (percent) during 2004 — 2008 ............................... 2-3 3-1 Analytical methods used to determine chemical and physical constituents in Mountain Island Lake during 2004 — 2008............................................................... 3-12 4-1 Densities (number/m2) of macroinvertebrates collected annually from Location B (upstream of RSS) from 2000 — 2008......................................................................... 4-4 4-2 Densities (number/m2) of macroinvertebrates collected annually from Location F (RSS discharge) from 2000 — 2008............................................................................. 4-8 4-3 Densities (number/m2) of macroinvertebrates collected annually from Location G (downstream of RSS) from 2000 — 2008.................................................................. 4-11 5-1 Number of individuals and number of species in winter electrofishing surveys from three locations (B, F, and G) in Mountain Island Lake, 1994 — 1999, 2000 — 2003, and 2004 — 2008................................................................................................ 5-8 5-2 Number of individuals and number of species in summer electrofishing surveys from three locations (B, F, and G) in Mountain Island Lake, 1991 —1993, 1994 — 1999, 2000 — 2003, and 2004 — 2008.......................................................................... 5-9 5-3 Number of individuals, biomass, and number of species collected during spring electrofishing of five 300-m transects downlake and five 300-m transects uplake of the RSS discharge canal in Mountain Island Lake, 1993 — 1997, 1999 — 2008...5-10 5-4 Number of individuals and number of species collected during spring electrofishing of ten 300-m transects in the vicinity of the RSS discharge canal in Mountain Island Lake, 1994 — 1997 and 1999, 2000 — 2003, and 2004 — 2008... 5-11 5-5 Number, percentage, average length, average density, and forage fish population estimates in Mountain Island Lake, 1993 — 1997, 1999 — 2008, from mid- September purse seine and hydroacoustic surveys ................................................... 5-12 vii LIST OF FIGURES Figure Title Page 1-1 Sampling locations on Mountain Island Lake. Duke Energy historical location identifiers are listed in parentheses............................................................................. 1-6 2-1 Average monthly CCW discharge temperatures (solid circles) and monthly range 3-23 3-19 daily average temperatures (open circles), displayed over days per month of RSS 3-23 3-20 coal-fired generation during 2004 — 2008................................................................... 2-4 2-2 Average monthly downstream temperatures (solid circles) and monthly range of 3-24 3-22 daily average temperatures (open circles)................................................................... 2-4 3-1 Winter profiles of temperature (°C; ■) and dissolved oxygen (mg/L; ❑) at the 3-25 3-24 Mountain Island Lake forebay, Location G.............................................................. 3-15 3-2 Summer profiles of temperature (°C; ■) and dissolved oxygen (mg/L; ❑) at the 3-25 3-26 Mountain Island Lake forebay, Location G.............................................................. 3-16 3-3 Semi-annual specific conductance profiles in Mountain Island Lake, 2004 — 3-26 2008........................................................................................................................... 3-17 3-4 Semi-annual pH profiles in Mountain Island Lake, 2004 — 2008 ............................. 3-18 3-5 Distribution of Mountain Island Lake specific conductance ..................................... 3-19 3-6 Distribution of Mountain Island Lake pH................................................................. 3-19 3-7 Distribution of Mountain Island Lake total alkalinity concentrations ...................... 3-19 3-8 Distribution of Mountain Island Lake turbidity........................................................ 3-20 3-9 Distribution of Mountain Island Lake total suspended solids concentrations........... 3-20 3-10 Distribution of Mountain Island Lake ammonia nitrogen concentrations ................. 3-20 3-11 Distribution of Mountain Island Lake nitrate+nitrite nitrogen concentrations......... 3-21 3-12 Distribution of Mountain Island Lake total nitrogen concentrations ........................ 3-21 3-13 Distribution of Mountain Island Lake soluble orthophosphate concentrations........ 3-21 3-14 Distribution of Mountain Island Lake total phosphorus concentrations ................... 3-22 3-15 Distribution of Mountain Island Lake calcium concentrations ................................. 3-22 3-16 Distribution of Mountain Island Lake magnesium concentrations ........................... 3-22 3-17 Distribution of Mountain Island Lake sodium concentrations . ................................. 3-23 3-18 Distribution of Mountain Island Lake potassium concentrations ............................. 3-23 3-19 Distribution of Mountain Island Lake chloride concentrations ................................. 3-23 3-20 Distribution of Mountain Island Lake sulfate concentrations ................................... 3-24 3-21 Distribution of Mountain Island Lake iron concentrations ....................................... 3-24 3-22 Distribution of Mountain Island Lake manganese concentrations ............................ 3-24 3-23 Distribution of Mountain Island Lake aluminum concentrations .............................. 3-25 3-24 Distribution of Mountain Island Lake silica concentrations ..................................... 3-25 3-25 Distribution of Mountain Island Lake arsenic concentrations .................................. 3-25 3-26 Distribution of Mountain Island Lake barium concentrations .................................. 3-26 3-27 Distribution of Mountain Island Lake copper concentrations ................................... 3-26 viii LIST OF FIGURES, Continued Figure Title Page 3-28 Distribution of Mountain Island Lake soluble copper concentrations ...................... 3-26 3-29 Distribution of Mountain Island Lake mercury concentrations ................................ 3-27 3-30 Distribution of Mountain Island Lake zinc concentrations ....................................... 3-27 3-31 Average annual RSS ash basin discharge and arsenic loading to Mountain Island Lake, 1992 —2008 ..................................................................................................... 3-28 3-32 Average annual RSS ash basin discharge and selenium loading to Mountain Island Lake, 1992 — 2008........................................................................................ 3-28 3-33 Distribution of arsenic concentrations in sediment fines sampled from Mountain Island Lake upstream (US) and downstream (DS) of the RSS ash basin outfall...... 3-29 3-34 Distribution of selenium concentrations in sediment fines sampled from Mountain Island Lake upstream (US) and downstream (DS) of the RSS ash basin outfall........................................................................................................................ 3-29 4-1 Density (number/m2) of macroinvertebrates collected annually during 2000 — 2008 from Mountain Island Lake..............................................................................4-15 4-2 Total number of macroinvertebrate taxa collected annually during 2000 — 2008 from Mountain Island Lake.......................................................................................4-15 4-3 Density (number/m2) of Oligochaeta, Diptera, and Corbacula collected annually during 2000 — 2008 from Location B in Mountain Island Lake ............................... 4-16 4-4 Density (number/m2) of Oligochaeta, Diptera, and Corbacula collected annually during 2000 — 2008 from Location F in Mountain Island Lake ................................ 4-16 4-5 Density (number/m2) of Oligochaeta, Diptera, and Corbacula collected annually during 2000 — 2008 from Location G in Mountain Island Lake ............................... 4-17 5-1 Number of fish (a), and fish species (b) collected from electrofishing a 100-m transect at each of three locations (B, F, and G) during winter electrofishing in Mountain Island Lake, 1994 — 2008......................................................................... 5-13 5-2 Number of fish (a), and fish species (b) collected from electrofishing a 100-m transect at each of three locations (B, F, and G) during summer electrofishing in Mountain Island Lake, 1994 — 2008......................................................................... 5-14 5-3 Selenium concentrations in muscle tissue from common carp (a), sunfish (b), and bass (c) from four locations (A, B, C, and G) in Mountain Island Lake, 1994 — 2008. The only complete common carp samples were Locations A (2004 — 2006) and C (2005)................................................................................................... 5-15 5-4 Arsenic concentrations in muscle tissue from common carp (a), sunfish (b), and bass (c) from four locations (A, B, C, and G) in Mountain Island Lake, 1994 — 2008. The only complete common carp samples were Locations A (2004 — 2006) and C (2005)................................................................................................... 5-16 ix LIST OF FIGURES, Continued Figure Title Page 5-5 Mercury concentrations in muscle tissue from common carp (a), sunfish (b), and bass (c) from four locations (A, B, C, and G) in Mountain Island Lake, 1995 — 2008. The only complete common carp samples were Locations A (2004 — 2006) and C (2005)................................................................................................... 5-17 5-6 Zinc concentrations in muscle tissue from common carp (a), sunfish (b), and bass (c) from four locations (A, B, C, and G) in Mountain Island Lake, 1997 — 2008. The only complete common carp samples were Locations A (2004 — 2006) and C(2005).................................................................................................................... 5-18 X CHAPTER 1 INTRODUCTION Riverbend Steam Station (RSS) is located northwest of Charlotte, in Gaston County, North Carolina on 25 -km Mountain Island Lake, one of the 11 Catawba River reservoirs (Figure 1- 1). The RSS began commercial service in 1929. Three of the oldest generating coal-fired units (1 — 3) were retired from service in the 1970s. The station presently has four operating coal-fired units (4 — 7), with a combined generating capacity of 454 MWe-net. Units 4 and 5 began commercial operation in 1952, and units 6 and 7 followed in 1954. In recent years RSS generation has typically been used to meet peak electric power demand, and as such, short-term and cyclic operation of selected units is common. In the 1990s, the four coal-fired units were modernized to improve reliability in light of their role in meeting electrical load peaks; the above -listed unit capacities reflect those modifications. Four additional combustion turbine units located at the RSS site, each of 30 MWe-net capacity, are not condenser -cooled and therefore do not reject heat to Mountain Island Lake. The RSS coal-fired units 4 — 7 utilize Mountain Island Lake as a source for once -through condenser cooling water (CCW). The CCW intake and discharge structures for RSS are located approximately 10.6 and 22.0 river km, respectively, downstream of Cowan's Ford Hydroelectric Station (CFHS; impounding Lake Norman). The RSS CCW discharge structure is approximately 2.7 km upstream of Mountain Island Hydroelectric Station (MIHS). Each generating unit has two CCW pumps that can be operated independently. Station operators run either one or two pumps per unit depending on intake water temperature, discharge temperature limits, and operational efficiency. The four RSS once - through -cooled units are capable of withdrawing CCW at a combined maximum rate of 18.2 m3/s (642 cfs). 1-1 REGULATORY CONSIDERATIONS Current National Pollutant Discharge Elimination System (NPDES; NCDENR 2005) permitted thermal limits for RSS CCW effluent, based upon an approved 316(a) thermal variance, are: • a monthly average CCW discharge (i.e , end -of -pipe) temperature limit of 35 °C (95 °F), and • a daily average downstream water temperature limit of 32.0 °C (89.6 °F) measured at Mountain Island Hydroelectric Station. The RSS NPDES permit stipulates additional delta temperature (AT) thermal effluent limits (i.e., maximum permissible temperature increase above ambient, or upstream conditions), based on the Environmental Protection Agency (EPA) nomograph (USEPA 1976), under specific operational conditions. The EPA nomograph limits are intended to protect warmwater fish from "cold shock" in the event of a winter season plant shutdown. These operational constraints (paraphrased below) were originally approved by the North Carolina Department of Environment and Natural Resources NCDENR in 1993, and incorporated into subsequent RSS NPDES permits, including the present permit: • when only one RSS control system is operating and the inlet water temperature ranges from 2.5 °C (36.5 °F) to < 12 8 °C (55 W), then the average daily discharge water temperature limit is two times the inlet temperature (°F) minus 23, and • when only one RSS control system is operating and the inlet water temperature is < 2.5 ° C (36.5 °F), then the average daily discharge water temperature limit is 10 cC (50 °F) Assessment of the potential influence of thermal discharges on biological communities is a key component of thermal discharge variances granted under section 316(a) of the Clean Water Act (CWA). The term "balanced, indigenous community" [40CFR125.71(c)] is synonymous with the term "balanced, indigenous population" in the CWA, and refers to a biotic community typically characterized by diversity, the capacity to sustain itself through cyclic seasonal changes, presence of necessary food chain species and a lack of domination by pollution tolerant species. Such a community may include historically non-native species introduced in connection with a program of wildlife management and species whose presence or abundance results from substantial, irreversible environmental modifications (USEPA 1977). 1-2 Pursuant to an agreement with the NCDENR, annual monitoring of macroinvertebrates and fish was initiated in 1990 at selected Mountain Island Lake locations in the vicinity of RSS. The objectives of this ongoing monitoring program were to assess macroinvertebrate and fish populations with respect to operation of RSS, and provide an indication of the suitability of approved thermal limits as defined in the RSS NPDES permit. Additionally, water quality and sediment samples were evaluated to provide information relevant to the biological assessment, but also to characterize potential impacts to the reservoir relating to the permitted RSS ash basin discharge. Water quality and biological samples are collected each year from established Mountain Island Lake sample locations (A — G) upstream, within the immediate influence of, and downstream of the RSS thermal discharge. Additionally, fish are collected from 10 shoreline segments in the vicinity of RSS in the spring (Table 1-1 and Figure 1-1). Finally, sediment samples are collected generally once per permit period at selected locations upstream and downstream of the RSS ash basin discharge. Conventional Duke Energy sample location numbers associated with Locations A through G are provided in Table 1-1 and Figure 1-1 for reference to previously submitted reports. Locations A and B are upstream of the RSS thermal discharge (Location A is also upstream of the station CCW intake) and for the purposes of the 316(a) monitoring program, these two locations were considered reference sites. Location C is immediately downstream of the RSS ash basin discharge. This location is used in conjunction with Location B to evaluate water quality and sediment chemistry in relation to the potential impacts of the RSS ash basin discharge. Location D is situated in the McDowell Creek arm of Mountain Island Lake and is utilized to characterize potential water quality impacts resulting from inflows from that extensively developed sub -catchment. Location E represents the area where Charlotte -Mecklenburg Utilities Department (CMUD) withdraws raw lake water for drinking water supply; water chemistry is sampled to assist in characterizing Mountain Island Lake water quality at the site. Location F is adjacent to the RSS CCW thermal discharge and is utilized to evaluate potential near -field effects on aquatic biota from heated effluents. Finally, Location G is situated at the Mountain Island Lake forebay and represents a far -field site for evaluating impacts of the RSS thermal discharge. Previous studies of this site have established that the existing limits on the thermal component of the discharge of RSS have assured the protection and propagation of balanced, indigenous populations in Mountain Island Lake (Duke Power Company 1994; Duke Power 2001 and 2004). This report presents results related to RSS operations from data collected 1-3 since submittal of the previous (2004) summary report and includes data collected from 2004 — 2008 (Note: January 2004 sediment trace element data were included in the previous report). Also, the 2004 — 2008 data are compared with other environmental monitoring programs conducted in this watershed. 1-4 Table 1-1. Biological and water quality monitoring locations on Mountain Island Lake. 1 Latitude/longitude data obtained from TOPOI USGS mapping software (National Geographic Holdings, Inc 2001) River km (mi) Report Duke County Description Assessments Upstream of Location Energy Mountain Latitude' Longitude' Designation Location Island N W No. Hydroelectric Station A 16.0 Gaston I Catawba River at entrance to Duck Cove Water quality 17.2 35° 22.894' 80° 58.817 Mecklenburg Sediments (10.7) Fish Mecklenburg Catawba River upstream and north of RSS ash Water quality 11.6 35° 22.358' 80° 57.363' B 2776 basin discharge; eastern side of lake Sediments (7 2) Macroinvertebrates Fish C 277.5 Gaston / Catawba River downstream of RSS ash basin Water quality 10.8 35° 22.009' 80° 57.085' Mecklenburg discharge and upstream of McDowell Creek Sediments (6 7) confluence, western side of lake D 277.3 Mecklenburg McDowell Creek cove, approx. 250 m upstream Water quality 100 350 22.240' 800 56.510' from Catawba River mainstem confluence 6.2 E 277.0 Mecklenburg Catawba River at Charlotte -Mecklenburg Water Water quality 5.8 35° 20.922' 80° 56 621' Treatment plant intakes 3.6 F 276.5 Gaston RSS condenser cooling water discharge canal Water quality 27 35° 21.320' 80° 58171' Macroinvertebrates (17) Fish G 275.0/ Gaston Mountain Island Dam forebay (275 0); Water quality 0.2-06 35° 20.069' 80° 59 203' 275.5 Cove upstream of the Mt. Holly Water Treatment Sediments (0.1-0.4) 35° 20 099' 80° 59.428' plant intake (275 5) Macroinvertebrates Fish 1 Latitude/longitude data obtained from TOPOI USGS mapping software (National Geographic Holdings, Inc 2001) TOM] map p— V on 03/17/09 from \ C `*" tpo' and •01m0a1tp8' 00°5900)0 W 80.50000,w 60°57000 W 80-56000'W WG5U 80°55000 W N r? '41C, �� Ir�� ' !L`O� � � L=:-' � ,yi' ��'- � V '•`.i i.�.-� e • � %� l �r _h�* 1. n� o��:i -y7if ��„ � r„� *13-d_•- F �'C' � ���� ,'��� •ff �����% �•.-" �a � �� � i � ., _,l� r tt'-' "'kk� i%'':rte °.�,�,� `f' i/;•• a j f� 's� � ���� � l ^ � .T 1ri ,fit• �' -t�,•, r � � 11��� t+� (� .-- � �� �s���1 •3%r ✓ �,� }-t` •> _ .2�z?., del:. � ''� t�^- /,'� ' ✓i�;, 1, �:,Y,lY�" !w� �r la'..};,_ _ ���S� ;uJ! �_ ✓fd�\\ 1 -;,, �� Gam. �:�m� �-�'.5�1 /tir>,\C' Y''�,ii,•,'7:,.'R��� �t „ Monitoring Locations Electrofishmg Locations J/ -�• �'';a'� .1 Lam_ ,� '`^+` � .��^� ` I l,� i �h - 4'� Y 11i11�� �� �`% �' ��(��'� � 1 C'tPr e �"` �, `�`�Ri�1' r'y� •!, �^, f� l :�-�'ti� \,l .,..,,,,It)' h�' s °\ $' _ �� y _� t4 f VNV 71J 1 "' 10. t n ^fo-- �• \ r��+'`�`� A 16 0 `' vY:L�iI 11 r�r�ll�_ - iv% dyi \ �..];� It s vl✓1:� f( ) i! �c` 21 • � - �b�,/ev � , �V `� 1 0 , L � -` r �� �i� c (``��"\�.r�1 G•r _`r' �/ 1 r B � ± i\ �' �/. '�.• y' y o,;4 , (277 3) 4 } J 1 C1 `!iG�,/'t i r i °a�Ge Ev nd am -,,: 1_ur'� �°`s~ �°f k F 1276 5) `,, s Z 1 G(2755/2750) ,"" °.w. • . I _.�`:��t w%>a" �" , (_,y r.r-t "" IWO ,v° � bt i ���� �'Y „ys ,-� .Ir• i' - x `rfg,jiry��'�r���+'�"J' };`, 7`'' ry`;rV��\ { r �j� Et 77 0) .'Af 1 `, 5. ,( a��t �' �w'!t� „��~-T i Tv�, �-Jl ^.,t..�•�''`,'�1'�`t{`7t�'� �.�.,,�.m� & .�-�� �t�'� t �� slartd '11711 ,,JI 00.59000 W 00.59000 W 80.57000 W 80°56000 W WG50400`550 O W Y �bllti I Figure 1-1. Sampling locations on Mountain Island Lake. Duke Energy historical location identifiers are listed in parentheses. 1-6 CHAPTER 2 STATION OPERATION METHODS Riverbend Steam Station (RSS) intake, discharge, and downstream water temperatures were continuously monitored during 2004 — 2008 to ensure thermal permit compliance. Thermal compliance data were obtained from National Pollutant Discharge Elimination System (NPDES) discharge monitoring reports. Station generating unit capacity data were compiled from the Duke Energy MicroGads and predecessor databases. Until April 9, 2008, RSS downstream water temperatures were measured at the Mountain Island Hydroelectric Station (MIHS) tailrace. During periods of very low Catawba River flow, however, particularly as experienced during recent drought years, MIHS generation was limited. In the absence of hydroelectric generation during warmer months, daytime temperatures in the quiescent tailwater pool could be appreciably warmed by incident solar radiation, in excess of upstream (upper pool) thermal conditions in deeper lake strata which correspond to MIHS penstock elevations. Therefore, Duke Energy sought and was granted permission from North Carolina Department of Environment and Natural Resources (NCDENR) to relocate the RSS downstream thermal compliance point upstream of the MIHS. On April 9, 2008 the downstream thermal sensor was relocated to the Mountain Island Dam forebay at a depth of 9 in; a depth that historical monitoring data indicated corresponded to tailwater temperatures during periods of hydroelectric generation. RESULTS AND DISCUSSION Station Operation The RSS has in recent years operated as a peaking facility, and operation during January 2004 through December 2008 was consistent with this role of peak electric load generation. Electric generation (from one or more coal-fired units) occurred on 81% of days between January 1, 2004 and December 31, 2008 (Figure 2-1). For the entire five-year period, RSS 2-1 coal-fired units operated at an average of about 48% of capacity (i.e., actual/potential station generation), ranging from 39.6% in 2004 to 56.3% in 2007 (Table 2-1). Month-to-month generation capacity factors varied substantially by season and year, depending on load demand and unit outages. Maximum monthly average capacity was achieved during the June — August period in each of the five years, reflecting greater utilization of RSS for periods of peak summertime electric demand. Thermal Compliance The thermal limits of the NPDES permit of RSS were not exceeded during the 2004 — 2008 period. The average monthly CCW discharge water temperature ranged from 14.9 °C (58.9 °F) in February 2005, to 34.7 °C (94.4 °F) in August 2007 (Figure 2-1). These temperatures followed a regular seasonal pattern and were within thermal ranges previously reported (Duke Power Company 1993, 1994; Duke Power 2004). Likewise, daily average downstream temperatures measured at MIHS reflected typical seasonal variability, ranging from 8.1 °C (46.5 °F) on January 29, 2004 to 31.9 °C (89.5 °F) on August 10, 2007 (Figure 2- 2). During 2004 — 2008, maximum average monthly CCW outlet and downstream daily average temperatures were recorded in August 2007. These thermal maxima were influenced by below normal total precipitation in August 2007 (1.04 cm versus a normal 9.45 cm), as well as appreciably warmer than normal ambient August 2007 air temperatures (587 cooling degree-days versus a normal of 415; NOAA 2008a). Compliance with EPA Nomograph -Related Thermal Limits The EPA nomograph thermal limits (see Chapter 1) apply during times of relatively low receiving stream temperatures, and when only one of the RSS coal-fired units control systems is operational. A nomograph limit is calculated by applying a maximum permissible temperature increase (OT) above ambient, i.e., CCW inlet temperature. These limits were established to protect resident warmwater fish from potential "cold shock" in the event of a unit trip or sudden unit shutdown, which would lead to rapid cooling in the portion of Mountain Island Lake affected by the RSS thermal plume. Coincident wintertime operation of more than one RSS control system frequently restricted the applicability of nomograph thermal limits. During 2004 — 2008, thermal effluent temperatures complied with applicable nomograph thermal restrictions at all times. 2-2 Table 2-1. RSS monthly units 4 through 7 (U4 - U7), monthly average (Av), and annual station coal-fired capacity factors (percent) during 2004 - 2008. N W 2004 2005 2006 2007 2008 Month U4 U5 U6 U7 Av U4 U5 U6 U7 Av U4 U5 U6 U7 Av U4 U5 U6 U7 Av U4 U5 U6 U7 Av Jan 668 661 763 277 580 357 404 419 423 404 00 00 51 54 29 179 164 324 543 325 594 53.8 592 589 581 Feb 708 672 743 00 50.3 174 454 322 390 339 304 308 452 462 394 469 520 637 625 574 464 45.5 50.5 589 511 Mar 299 449 464 29 299 715 682 472 773 654 364 341 423 519 422 313 312 320 313 315 413 644 69.2 64.2 610 Apr 594 51.1 45.2 688 56.3 305 57.9 0.0 655 374 67.3 52.5 74.5 775 693 473 493 578 539 52.7 76.1 78.8 74.2 825 78.0 May 540 704 740 774 701 423 387 16.9 17.4 268 34.9 36.3 42.6 46.9 410 643 637 647 645 64.4 689 637 654 657 659 Jun 340 658 74.5 80.2 66.0 496 485 63.9 642 578 68.4 646 673 767 697 587 579 615 62.4 60.4 85.3 83.6 750 822 81.0 Jul 422 49.8 630 653 56.6 717 674 645 578 646 780 789 810 789 793 775 725 710 682 718 701 59.2 668 512 61.4 Aug 524 457 454 581 506 75.6 727 732 785 751 78.9 74.7 81.3 74.3 774 788 763 757 76.1 76.6 47.2 43.0 570 544 513 Sep 119 10.0 265 268 20.2 619 62.1 68.9 68.7 66.0 3.7 4.1 19.0 12.9 110 63.0 32.7 63.4 73.4 59.9 24.4 23.8 293 33.1 28.2 Oct 00 0.0 7.9 10.2 5.1 35.7 421 368 480 410 59.7 603 570 122 451 781 52 439 755 522 00 00 10.4 127 6.6 Nov 0.0 0.0 0.4 3.0 08 20.9 25.6 149 00 138 665 657 6.0 699 496 529 658 698 612 629 104 124 262 382 23.6 Dec 136 19 159 135 118 469 410 375 3.3 30.1 355 352 462 417 404 563 595 569 46.7 543 173 16.0 257 269 223 Annual 39.6 46.2 47.3 56.4 49.0 N W 45 113 Monthly average limit 40 4 q p 104 35 4Q1 IQ 4414 QQ 95 30 86 U 25 0 0 CD %a y CD E 20 68 m 9 TI 15 59 10 50 5 41 O - — - -- -- — 32 30 - -----""F'-- --"- -- --'�—' ----- - --- — '----'- - - ----+-'-- - ----- 25 —'—�- --}— — --- - w , 5 — -- N 10 0 5 0 2004 2005 2006 2007 2008 Date Figure 2-1. Average monthly CCW discharge temperatures (solid circles) and monthly range daily average temperatures (open circles), displayed over days per month of RSS coal-fired generation during 2004 — 2008. 35 32 30 25 U uD 20 m `m CL E 15 F� 10 5 95 86 77 m 68 3 CD CD c 59 50 41 0 I- i 32 2004 2005 2006 2007 2008 Figure 2-2. Average monthly downstream temperatures (solid circles) and monthly range of daily average temperatures (open circles). 2-4 CHAPTER 3 WATER AND SEDIMENT CHEMISTRY MATERIALS AND METHODS Collection of Field Parameters, Water, and Sediment Chemistry Samples Mountain Island Lake water quality was monitored at six locations (A, B, C, D, E, and G; Figure 1-1 and Table 1-1) semiannually (summer and winter) during 2004 — 2008. Sample locations ranged (as ordered above) from the furthest upstream site at Cowan's Ford Hydroelectric Station (CFHS) tailwater to the furthest downstream site at the Mountain Island Hydroelectric Station (MIHS) forebay. Locations were the same as those monitored in prior years (Duke Power Company 1994; Duke Power 2001 and 2004). In-situ analyses were performed by Duke Energy Scientific Services personnel? At each location, vertical profiles of in-situ parameters (temperature, dissolved oxygen, pH and specific conductance) were collected with a Hydrolab DataSondeo. Water samples for the laboratory analyses listed in Table 3-1 were collected with a Kemmerer bottle at surface (0.3 m) at each location, and approximately one meter above bottom at the MIHS forebay (Location G). Samples for soluble nutrients (i.e., ammonia -N, nitrtate+nitrite-N, and orthophosphate) were filtered (0.45-µm) in the field. Additional samples collected for the analysis of soluble copper, beginning in 2005, were also field - filtered. All samples were preserved (acidified or iced) in the field immediately following collection. Mountain Island Lake sediment sampling was conducted on March 6, 2008 (data from sediment samples collected January 30, 2004 were summarized previously; Duke Power 2004). Sediment cores were obtained from four locations (A, B, C, and G; Figure 1-1 and Table 1-1), including an upstream reference site (Location A), locations immediately upstream and downstream of the ash basin outfall (Locations B and C, respectively) and the MIHS forebay (Location G). Ten replicate cores were obtained from each location with a K- 2 The Duke Energy Scientific Services organization is presently certified by the North Carolina Division of Water Quality (DWQ) under the Field Parameter Certification program (certificate number 5193) However, all field chemistry data represented in this report are uncertified data; i.e., not collected for effluent compliance monitoring purposes. 3-1 B° gravity corer, or in shallower depths, a hand corer fitted with cellulose acetate butyrate core liner tubes. Upon collection, sediment cores were sealed with polyethylene end caps, with site water overlaying the intact water -sediment interface. Cores were maintained in an upright position to preserve the sediment -water interface, stored on ice, and subsequently refrigerated upon return to the laboratory. Analytical Methods Analytical methods and sample preservation techniques employed during 2001 — 2005 are summarized in Table 3-1. Since 2001, trace element concentrations of water samples have been analyzed as "total recoverable" elemental concentration, which incorporates a dilute acidic digestion of the sample (USEPA 1994). This technique was distinct from the analytical method for trace elements employed during the period 1988 — 2000, when acid - preserved samples were analyzed by atomic absorption spectroscopy direct injection, i.e., samples were not acid -digested. With minor exceptions, laboratory water quality analyses were performed by Duke Energy Corporate EHS Analytical Laboratory, Huntersville, NC (North Carolina DWQ Laboratory Certification program, certificate number 248). In several instances, selected parameters were analyzed by an alternate state -certified commercial laboratory. Prism Laboratories, Inc., Charlotte, NC (NC DWQ certificate number 402) provided turbidity analyses (except for February 2004 samples), total solids analyses for 2005 — 2008, total suspended solids for 2005 samples, and mercury analyses for February 2007 and February 2008 samples. Because the uppermost sediment strata was of primary concern in evaluating recent deposition and trace element bioavailability, sediment core samples were processed in the laboratory to remove surficial material (i.e., silt + clay fractions) from the uppermost stratum of each core. Upon return to the laboratory, fine-suspendable sediments were siphoned from the uppermost 2 mm of each sediment core, sieved through a 63-µm plastic (Nitex°) screen, and then deposited onto a pre -weighed 0.45-µm Millipore® acetate membrane filter. Filters were subsequently dried at room temperature and analyzed by non-destructive neutron activation analysis at the Nuclear Services Laboratory, North Carolina State University, Raleigh, NC. Quality assurance measures for dry weight -based selenium and arsenic concentrations (expressed as µg element/g sediment) employed analyses of filter blanks, internal standards, and National Institute of Standards and Technology or International Atomic Energy Agency reference materials for calibration. 3-2 Data analyses employed both statistical and graphical methods. Time series plots were used to assess seasonal and inter -annual trends in CCW and lake temperatures. Box and whisker plots (showing median, 25% and 75% quartiles, and data range) were produced for selected water chemistry analytes, by sample location and pre -defined year groupings, to permit temporal and spatial comparisons with historical data. Trace element concentrations in Mountain Island Lake sediments were analyzed by non -parametric procedures (Wilcoxon rank sum and Kruskal-Wallis tests) for effects by year and location, respectively (SAS Institute Inc. 2002 — 2004) where statistical treatment of analytical results was not limited by the prevalence of below -detection values. In all instances where analyte concentrations were less than the laboratory reporting limit, the reporting limit value was represented graphically. RESULTS AND DISCUSSION Water Quality Because of the relatively small catchment and short hydrologic retention time of Mountain Island Lake (e.g., < 1 to 12 days; Bales et al. 2001), the thermal structure of the reservoir is substantially influenced by the linkage of controlled releases from the upstream CFHS and outflows from MINS, combined with the thermal plume from RSS. A submerged weir immediately upstream of Cowans Ford Dam results in inflows to Mountain Island Lake during summer hydro generation that are thermally representative of the warmer Lake Norman epilimnion. The high flow-through rates, small reservoir catchment, and relatively shallow reservoir morphometry all serve to limit the establishment of a strong thermal gradient, particularly in areas of the lake upstream of RSS. Typical seasonal trends during 2004 — 2008 were observed for Mountain Island Lake temperature and dissolved oxygen (Figures 3-1 and 3-2). Forebay thermal profiles were similar to results of previous monitoring (Duke Power Company 1994; Duke Power 2001, 2004). During the summers of 2004 — 2008, the deeper regions of the reservoir became weakly stratified thermally, and dissolved oxygen levels declined gradually with increasing depth below the uppermost mixed layer (i.e., top 3 to 5 m) of the water column (Figure 3-2). As in previous years, the occurrence of summertime hypolimnetic anoxia at the MIHS forebay was variable year-to-year, with anoxic conditions occurring most years at elevations in the reservoir corresponding to the lower limit of the MIHS penstocks, i.e., normally in the bottom 3 to 4 meters of the water column. Thermal stratification was slightly less 3-3 pronounced for the August 2005 sampling compared to other years, and can be linked to lack of anoxic conditions at the time in those bottom waters. Surface specific conductance (Figures 3-3 and 3-5) ranged lake -wide from 49.9 to 227 µS/cm, with maximum values typically occurring in the McDowell Creek cove (Location D). This finding is consistent with recent monitoring trends and is in agreement with data reported by other researchers (Bales et al. 2001; Buetow 2003). McDowell Creek receives a substantial point source discharge from the recently enlarged Charlotte -Mecklenburg Utilities 12 -MGD McDowell Creek Wastewater Treatment Plant (WWTP), as well as non - point source contributions due to increasing (primarily residential) development in the watershed. Specific conductance values measured in the lower water column (i.e., 3 to 4 in deep) at Location D were frequently among the greatest encountered lake -wide. Near - surface specific conductance values at this location were exceptionally greater in February 2005, however, likely reflecting warmer, buoyant inflows from McDowell Creek relative to cooler ambient lake temperatures at the time of sampling (Figure 3-3). Based on conductance profiles, elevated dissolved solids inflows from McDowell Creek frequently appear to suffuse into the lake at depths below 3 meters. Elevated summer specific conductance values observed in the deepest waters of Mountain Island Lake forebay (Location G) are primarily linked to increased concentrations of the dissolved fraction of redox -sensitive metals (e.g., reduced iron and manganese), reflective of the influence of thermal stratification and seasonal anoxia at that particular site. From a temporal perspective, slightly elevated specific conductance was observed lake -wide during 2008, with August 2008 values similar to increased conductance levels encountered during the 1998 — 2002 regional drought. A drought period spanning spring 2007 through the summer of 2008 likely contributed to the elevated dissolved solids, driving the conductance readings upward (NCDENR 2009). However, marginally increased Lake Norman dissolved solids concentrations, linked to a new discharge of flue gas desulfurization wastewater at Marshall Steam Station on Lake Norman, may also be a contributor to recently elevated lake -wide conductance values (Duke Energy, unpublished data). In-situ pH (Figures 3-4 and 3-6) in the main channel of Mountain Island Lake tended to be circum -neutral, with a median pH of 7.1, and a range of pH 6.1 to 7.9 during 2004 — 2008. A general increase in reservoir -wide pH, and to some extent, total alkalinity (see discussion below; Figure 3-7) is apparent over the 19 years of monitoring summarized in this report. These gradual increases may be related to slight increases in calcium (Figure 3-15) as well as decreases in sulfate concentrations (Figure 3-20) over a similar time period. While added 3-4 calcium inputs are likely attributable to increased Mountain Island Lake and Catawba River Basin watershed development over the past two decades, similarly timed reductions in sulfate concentrations in Southeastern surface waters have been observed. These sulfate reductions are thought to be related to lower sulfate deposition attributable to air emissions control measures implemented at numerous up -wind coal -electric generating facilities (USEPA 2003). Surface (0.3 m) pH values ranged from 6.6 to 7.9 lake -wide. Lowest pH values were observed during the August 2005 sampling. Drought -associated summer 2007, and particularly 2008 pH values in the McDowell Creek cove (Location D) ranged from 7.5 to 7.9, displaying a tendency toward slightly more alkaline conditions compared to other lake - wide sample locations. Likewise, forebay (Location G) euphotic zone (e.g., depths < 3 m) pH values were marginally more alkaline (pH 7.4 to 7.8) in August 2008 than the earlier 2004 — 2007 samplings. These forebay pH values were coincident with slightly supersaturated dissolved oxygen concentrations (e.g., 109 to 111%) measured at 0.3 to 3 m depth and were probably linked to increased densities of phytoplankton and resultant photosynthetic activity occurring due to the warm and relatively dry weeks prior to the August 20 sampling date (NOAA 2008b). Total alkalinity as CaCO3 ranged from 12 to a maximum of 60 mg/L (the latter value from a forebay bottom sample in 2008) during 2004 — 2008 (Figure 3-7). Greatest alkalinity concentrations lake -wide were measured during the August 5, 2004 sampling, ranging from 23 to 29 mg CaCO3/L. From a spatial perspective, total alkalinity values associated with the McDowell Creek cove (ranging from 12 to 29 mg CaCO3/L) were usually slightly elevated with respect to surface (0.3 m) samples collected from other Mountain Island Lake locations. As discussed above, total alkalinity concentrations have displayed a long-term, but very slight increasing trend over the years of this monitoring program. While some of the noted increase is undoubtedly linked to drought conditions prevalent during several of the years over the past decade, other watershed -linked contributions may also be important, given the dynamic changes in land use and population over time. Mountain Island Lake turbidity ranged from 1.6 to 16.5 NTU in 2004 — 2008 (Figure 3-8). No samples exceeded the applicable state water quality standard (25 NTU; NCDENR 2007a). Reservoir -wide, turbidity was slightly less spatially variable, compared to prior reporting periods. Total suspended solids (TSS) concentrations were also generally low (ranging from 1 to 10 mg/L for mainstem locations) during 2004 — 2008 (Figure 3-9). A maximum TSS concentration of 16 mg/L occurred in the McDowell Creek arm of Mountain 3-5 _ Island Lake during the February 2007 sampling, a site where greatest TSS concentrations have frequently been observed in the past (Bales et al. 2001; Duke Power 2001, 2004). Nutrient concentrations throughout 2004 — 2008 reflected historical trends and were consistent with formerly documented, time -variable transitions between oligotrophic and mesotrophic water quality classifications for Mountain Island Lake (USEPA 1975; Weiss and Kuenzler 1976; Bales et al. 2001; Duke Power 2001; Buetow 2003; NCDENR 2004). Ammonia -nitrogen (Figure 3-10), nitrate+nitrite (Figure 3-11), and total nitrogen concentrations (Figure 3-12) were similar to previous years. Maximal ammonia -nitrogen concentrations were consistently measured during summer in the deeper waters of the MIHS forebay; Location G (maximum of 1.3 mg NH3-N/L August 20, 2008). This was the only sample location where near -bottom samples were obtained in this monitoring program. Seasonally elevated ammonia -nitrogen concentrations noted for deep samples collected at this site can be linked to anoxia -induced strongly reducing conditions, which result in an increased release of nutrients from lake sediments, as well as limitation of oxygen -dependent microbial nitrification processes. Such seasonal increases in ammonia -nitrogen concentrations have been reported previously in Mountain Island Lake, and other Catawba - River reservoirs (Bales et al. 2001; Duke Power 2004). These seasonal increases in the reservoir hypolimnetic ammonia -nitrogen were also reflected in 2004 — 2008 total nitrogen concentrations. Lake -wide, phosphorus concentrations were similar to those observed in recent years. Concentrations of soluble orthophosphate (Figure 3-13) and total phosphorus (Figure 3-14) in the McDowell Creek arm of Mountain Island Lake (Location D) have for many years provided a consistent indication of nutrient loading via the McDowell Creek WWTP. Bales et al. (2001) observed, based on 1996 — 1997 monitoring, that on average approximately one half of the loading of total phosphorus to Mountain Island Lake entered the reservoir from McDowell Creek. Six individual exceedances of the Mecklenburg County Water Quality Program (MCWQP) action level (0.04 mg/L) for total phosphorus were reported for the McDowell Creek arm of Mountain Island Lake during 2007, as well as a sample exceeding the action level in the Gar Creek arm of the lake, during the same drought -impacted year (Buetow 2008). During 2004 — 2008, both orthophosphate and total phosphorus concentrations were reduced in the McDowell Creek arm with respect to prior years of monitoring. Loading of nutrients to Mountain Island Lake during 2004 — 2008, while reduced overall, remained evident from spatial trends in nitrate+nitrite (Figure 3-11) and total phosphorus 3-6 (Figure 3-14) concentrations. As for phosphorus, the MCWQP action level for nitrate+nitrite (0.65 mg/L) was exceeded on three occasions in the McDowell Creek arm of the lake during 2007; no exceedances were observed elsewhere in the reservoir (Buetow 2008). McDowell Creek was listed as impaired for biological integrity in the most recent (2006) 303(d) evaluation of state water quality use attainment (NCDENR 2007b). Continuing nutrient enrichment and excessive solids loading from the McDowell Creek WWTP and the extensively developed catchment further upstream undoubtedly contribute to this impairment. During 2004 — 2008, concentrations of major cations, including calcium (Figure 3-15), magnesium (Figure 3-16), sodium (Figure 3-17), and potassium (Figure 3-18); and anions, including chloride (Figure 3-19) and sulfate (Figure 3-20) were generally consistent with previous trends. Median concentrations of these major dissolved ionic constituents, as for specific conductance (Figure 3-5), were slightly lower than the marginally elevated concentrations observed during the prolonged 1998 — 2002 regional drought. As discussed previously for calcium, long-term temporal trending appears to also indicate a slight increase in magnesium concentrations over the years 1990 — 2008, whereas sulfate concentrations appear to be in slight decline over the same timeframe. Mountain Island Lake iron (Figure 3-21) and manganese (Figure 3-22) concentrations during 2004 — 2008 were typical of previous years. Surface concentrations of the two metals remained low and showed minimal variation among the locations. However, as is commonly observed, substantially elevated summertime iron and manganese concentrations in the minimally oxygenated water near the lake bottom at the forebay location (G) were evident, as expressed by maxima on Figures 3-21 and 3-22 (note log concentration scale). Aluminum concentrations (Figure 3-23) have, over time, displayed a clear decreasing trend. It is possible, although presently unconfirmed for this site, that the observed decreases in aluminum may be linked to reduced regional atmospheric sulfate deposition and lessened watershed acidification over recent years, due to the increasing implementation of coal-fired power plant emission controls. Similar aluminum concentration reductions have been demonstrated in northeastern U.S. lakes in response to power plants air emission controls and reduced acid deposition of sulfur (Warby et al. 2008). Soluble silica concentrations (Figure 3-24) were comparable to long-term monitoring data. Median silica concentrations were marginally greater in the McDowell Creek cove (Location D) and further down -lake mainstem sites (Locations E and G) relative to further up -lake. 3-7 Aqueous concentrations of trace elements were typically near or below laboratory reporting limits during 2004 — 2008. No spatial trends were evident in the lake -wide trace element concentrations, with the exception that samples collected from the lake bottom at the MIHS forebay (Location G) yielded 2004 — 2008 maximum concentrations of total recoverable arsenic (maximum of 11.4 gg/L August 20, 2008; Figure 3-25) and barium (maximum of 0.14 mg/L; Figure 3-26). A maximum total recoverable copper concentration of 5.49 µg2 was measured at surface (0.3 m) at the forebay location (G) August 5, 2004 (Figure 3-27). Of these three trace elements, only a single August 2008 lake forebay (Location G) bottom sample exceeded a state water quality standard (arsenic; 10 gg/L based on human health) or action level (NCDENR 2007a). Total recoverable cadmium, chromium, lead, nickel, silver, and selenium concentrations consistently remained below method reporting limits (Table 3- 1) and applicable water quality standards or action levels throughout 2004 — 2008, and are not depicted graphically. Two mercury samples exceeded laboratory reporting limits during 2004 — 2008. Mercury in a sample collected August 5, 2004 from the lake mainstem at the CMUD water intake (Location E) had a concentration of 0.35 µg/L, and a second sample collected August 4, 2005 from the bottom at the MIHS forebay (Location G) yielded a concentration of 0.32 gg/L. The cause of this pair of elevated mercury concentrations, which exceeded water quality standard of 0.12 gg/L, was undetermined (NCDENR 2007a). RSS Ash Basin Effluent Selenium and Arsenic Mass Loading Estimates For the five-year period, 2004 — 2008, loading of arsenic (Figure 3-31) from the NPDES- permitted RSS ash basin discharge averaged 895 g/day, and selenium loading (Figure 3-32) averaged 47.5 g/day. As described previously (Duke Power 2001), the post -1995 estimated increase in arsenic or selenium 'loading, depicted in Figures 3-31 and 3-32, should be considered artifacts of modified discharge flow calculations implemented in 1995, rather than being attributed to any operational changes at RSS. Estimates of arsenic mass loading to Mountain Island Lake were near or below historical averages, ranging from 336 g/day (2007) to 1,556 g/day (2006). Arsenic loading increased approximately two -fold over the years 2004 — 2006, after a minimum level of loading was achieved in 2003. Subsequently in 2007 and 2008, loading was substantially reduced to levels not observed in recent years. Arsenic loading reductions in 2003 were in part linked to significantly greater precipitation event -driven watershed contributions to the ash pond (Duke Power 2004). Reductions in effluent arsenic concentrations driving the 2007 — 2008 3-8 loading estimates lower, however, may be related to changes in coal supply, as the reductions show no clear linkage with RSS coal-fired electric generation capacity (Table 2-1). Mass loading of selenium via the RSS ash basin outfall displayed somewhat less inter -annual variability, in contrast to arsenic. Annual average selenium loading ranged from 36 g/day (2004) to 56 g/day (2006). As observed previously, selenium loading from the ash pond effluent appeared more closely linked to effluent discharge rates than did arsenic loading rates (Duke Power 2001, 2004). Mean annual Se concentrations in ash pond effluents during 2004 — 2008 were only marginally above the laboratory reporting limit, ranging from 2.3 to 3.1 µg/L. Sediment Elemental Anal Neutron activation analysis of surficial fine (< 63 µm; silt+clay fraction) Mountain Island Lake sediments sampled March 6, 2008 resulted in arsenic concentrations of similar magnitude as observed previously at the two sites upstream of the RSS ash basin discharge (Locations A and B), but increased arsenic concentrations down -lake (Locations C and G), compared to previous years (Figure 3-33). Arsenic results for sediments collected immediately downstream of the RSS ash basin discharge (Location C) were particularly elevated in 2008 with respect to historical data, with a median arsenic concentration of 77.7 µg/g, i.e., more than three -fold the median 2004 arsenic concentration (see following discussion). Results of Kruskal-Wallis tests showed that for all 1988 — 2008 sediment samples combined, sample location had a highly significant effect (P < 0.001) on the magnitude of arsenic concentrations in sediment fines. For 2008 samples, Wilcoxon rank sum scores indicated arsenic concentrations in fine surficial sediments collected at the two sites downstream of the RSS ash basin outfall were significantly (Location C: P < 0.001; Location G: P < 0.01) greater than up -lake concentrations. Atypically elevated sediment arsenic concentrations from Location C, immediately downstream of the RSS ash basin outfall are thought to be an artifact of a slight change in the sampling site in 2008. Sediments sampled from the customary sites within the river channel in recent years have been exposed to substantial currents during upstream CFHS generation, so that samples frequently yield primarily coarse sand (virtually no fines present). This was particularly the case at Location C in 2008. To facilitate collection of sediment fines, an area was sampled approximately 250 in upstream, very near to the mouth of a cove where RSS ash pond effluent flows into the lake. Presumably, over the years, relatively arsenic -rich ash pond effluent -associated particulates have settled in this cove, including the region sampled 3-9 near the point of confluence with the river channel. Therefore, the 2008 Location C samples may be considered largely indicative of material entering the mainstem from the cove impacted by RSS ash basin effluents, rather than indicative of the mainstem of the lake itself (where deposits of sediment fines were virtually absent in 2008). The scarcity of sediment fines in the river channel at Location C is linked, conversely, to the gradual increases observed in sediment arsenic concentrations at the Mountain Island Lake forebay (Location G). Lower velocities encountered in this deepest portion of the lake facilitate an ultimate sink for fine particulates. Location G trace element analyses therefore serve to provide a window on the long-term arsenic mass loading from all upstream sources, including the RSS ash basin outfall. Similar to the 2004 sampling, sediment selenium concentrations (Figure 3-34) from samples collected in 2008 were mostly below sample -specific detection limits (i.e., 33 of 40 samples), so statistical methods were not employed for this analyte. (Selenium concentrations less than detection limits were represented graphically as the detection limit in Figure 3-34.) No overall location -dependent relationship was apparent in the combined set of 1988 — 2008 samples for selenium. This observation is consistent with the relatively low concentrations of selenium typical of the RSS ash basin discharge. CONCLUSIONS Water quality in Mountain Island Lake during 2004 — 2008 was similar to previous years. Seasonal thermal stratification, dissolved oxygen concentrations, and pH were representative of historical ranges. Specific conductance values had increased by the end of the monitoring period in 2008, achieving values similar to those recorded during the drought earlier in the decade. Lake -wide, concentrations of major nutrients, similar to historical levels, indicated spatial variability, but continued to result in Mountain Island Lake being classified as oligotrophic to mesotrophic during 2004 — 2008. Typically elevated concentrations of dissolved and suspended material, as well as nutrients entering the lake from the McDowell Creek drainage, were slightly lessened during 2004 — 2008. This impaired tributary, however, continued to produce a localized degradation of water quality, typically limited to the McDowell Creek cove of the lake. Mass loading of selenium and arsenic from the RSS ash basin outfall during 2004 — 2008 was similar to historical trends, except for reductions observed in arsenic loading over the 2007 — 2008 period. Mountain Island Lake concentrations of aqueous trace elements remained generally low, consistent with low levels 3-10 of turbidity and suspended solids. One sample (total recoverable arsenic of 11.4 µg/L, collected in August 2008) yielded the singular exceedance of a state water quality standard or action level for trace elements during 2004 — 2008. March 2008 arsenic concentrations in fine surficial sediments were elevated downstream of RSS with respect to uplake concentrations, with samples just downstream of the RSS ash basin effluent confluence yielding the highest concentrations. While the 2008 samples were likely influenced by a slight relocation of the sampling site due to difficulty in obtaining material other than coarse sand at the former location, MIHS forebay sediment sampling continues to indicate a slight, gradual increase in arsenic concentrations over recent years. Sediment concentrations of selenium were consistently low lake -wide. As a whole, 2004 — 2008 Mountain Island Lake water and sediment chemistry continue to indicate the waterbody remains capable of sustaining balanced and indigenous aquatic communities. Trace element concentrations measured in Mountain Island Lake common carp, sunfish, and largemouth bass since 1988 remain well below values of regulatory concern (Chapter 5). 3-11 Table 3-1. Analytical methods used to determine chemical and physical constituents in Mountain Island Lake during 2004 — 2008. Parameter Method (EPA/APHA)3 Preservation Reporting Limit Alkalinity, Total Total inflection point titration 4'C 0.01 meq/L EPA 310 1 as CaCO3 Aluminum Atomic emission/ICP 0 5% HNO3 0 05 mg/L EPA 200.7 Arsenic, Total ICP Mass Spectrometry 0 5% HNO3 2 0 pg/L Recoverable EPA 200.8 1 0 pg/L4 Arsenic, Sediment Neutron Activation Analysis 4 °C 0 0002 pg/g Barium Atomic emission/ICP EPA 200.7 Cadmium, Total ICP Mass Spectrometry Recoverable EPA 200.8 Calcium Atomic emission/ICP EPA 200 7 Carbon, Total Organic EPA 415.1 Chloride Conductance, Specific Chromium, Total Recoverable Copper, Dissolved Copper, Total Recoverable Iron, Total Recoverable Lead, Total Recoverable Magnesium Ion Chromatography EPA 300 0 Temperature -compensated nickel or graphite electrode APHA 2510 ICP Mass Spectrometry EPA 200 8 ICP Mass Spectrometry EPA 200.8 ICP Mass Spectrometry EPA 200.8 Atomic emission/ICP EPA 200.7 ICP Mass Spectrometry EPA 200 8 Atomic emission/ICP EPA 200.7 0 5% HNO3 0 5% HNO3 0 5% HNO3 4 °C, 0 5% H2SO4 4 °C in-situ 0 5% HNO3 0 5% HNO3 0 5% HNO3 0 5% HNO3 0.5% HNO3 0 5% HNO3 3 References 1 USEPA 1983 2. APHA et al. 1998 4 August 2008 only 5 Optimal conditions, no interfering elements in sample matrix 6 Instrument sensitivity furnished in lieu of laboratory reporting limit sample 0 005 mg/L 0 5 pg/L 1 0 pg/L4 0 03 mg/L 0 1 mg/L 10 mg/L 0 1 pS/cm6 1 0 pg/L 2 0 pg/L 1 0 pg/L4 2 0 pg/L 1 0 pg/L4 0.01 mg/L 2.0 pg/L 0.03 mg/L 3-12 Table 3-1. (Continued). Parameter Method (EPA/APHA) Preservation Reporting Limit Manganese ICP Mass Spectrometry 0 5% HNO3 1.0 pg/L EPA 200 8 Nitrogen, Ammonia EPA 350 1 40C 0 02 mg/L 0 5% H2SO4 Nitrogen, Nitrate+Nitnte EPA 353 2 40C 0 02 mg/L 0 5% H2SO4 Nitrogen, Total EPA 351 2 40C 0.1 mg/L Kjeldahl 0 5% H2SO4 Nickel ICP Mass Spectrometry 0 5% HNO3 2 0 pg/L EPA 200 8 Phosphorus, EPA 365 1 4 °C 0 005 mg/L Orthophosphate Phosphorus, Total EPA 365 1 40C 0 01 mg/L 0 5% H2SO4 Oxygen, Dissolved Temperature -compensated in-situ 0 01 mg/L6 polarographic cell APHA 4500-0-G pH Temperature -compensated in-situ 0 01 units glass electrode APHA 4500-H+ Potassium Atomic emission/ICP 0 5% HNO3 0 25 mg/L EPA 200.7 Selenium ICP Mass Spectrometry 0 5% HNO3 2.0 pg/L EPA 200.8 1 0 pg/L4 Selenium, Sediment Neutron Activation Analysis 40C 0 02 pg/g sample Silica (as Si) APHA 4500Si-F 40C 0 5 mg/L Silver ICP Mass Spectrometry 0 5% HNO3 0.5 pg/L 1 0 pg/L4 EPA 200 8 Sodium Atomic emission/ICP 0 5% HNO3 1.5 mg/L EPA 200 7 Solids, Total Gravimetric, dried at 103-105 °C 4 °C 20 mg/L EPA 160 3 12 mg/L' Solids, Total Gravimetnc, dried at 103-105 °C 4 °C 0 10 mg/L Suspended EPA 160 2 / APHA 2540 D Sulfate Ion Chromatography 40C 1 0 mg/L EPA 300 0 ' August 2005; 2006 samples 8 Modified Seston method for 1-L samples 3-13 Table 3-1. (Continued). Parameter Method (EPA/APHA) Preservation Reporting Limit Temperature NTC Thermistor in-situ 0 01 °C6 APHA 2550 Turbidity Turbidimetric 4 °C 0 05 NTU EPA 180 1 Zinc ICP Mass Spectrometry 0 5% HNO3 1 0 pg/L EPA 200 8 3-14 2004 2005 0 5 10 15 20 0 5 10 15 20 0 5 E L 10 CL 0) ❑ 15 20 0 0 5 L 10 Q a� 15 20 2006 2007 5 10 15 20 0 5 10 2008 0 5 10 15 20 0 5 15 20 Z 10 0. 0) 15 20 Figure 3-1. Winter (February) profiles of temperature (°C; ■) and dissolved oxygen (mg/L; ❑) at the Mountain Island Lake forebay, Location G. 3-15 E E 2004 0 5 10 15 20 25 30 35 0 5 15 2005 0 5 10 15 20 25 30 35 0 20 2006 0 5 10 15 20 25 30 35 0 5 15 20 E 2007 0 5 10 15 20 25 30 35 0 5 L 10 a CD 1s 20 2008 0 5 10 15 20 25 30 35 0 5 L 10 Q 0) 15 20 ' Figure 3-2. Summer (August) profiles of temperature (°C; ■) and dissolved oxygen (mg/L; ❑) at the Mountain Island Lake forebay, Location G. 3-16 Location A µS/cm 0 25 50 75 100 125 150 1i5 200 225 250 0 2 3 4 L 5 CL 6 7 8 9 Location C µS/cm 0 25 so 75 100 125 150 '175 200 225 250 0 P 9 S Feb 2004 __�--Aug 2004 4 Feb 2005 2 _ _—o-�Aug2005 Feb 2006 .vAug2006 8 - _ - - - Feb 2007 —f'Aug2007 + Feb 2008 `� • Aug 20081 CL e N 0 6 - _ _ _ _ _ _ _ g- - - _ _ _ _ - _ _ x Location E µS/cm 0 25 50 75 100 125 150 175 200 225 250 0 • o F 211 7 - b� Aug 2004 Feb2005 2a • a d n= n Aug 2005 Feb 2006 3 �- s>4_ - Aug 2006 E 4 L 5 O. (1) s 7 8 9 1n Location B µS/cm 0 25 50 75 100 125 150 175 200 225 250 0 } o Fe62004 1 _—_-- -4 - ____________ _ __ —0_Aug 2004 c= Feb 2005 2 • s -Aug 2005 o Feb 2006 3 —_—_ y _ _ _ _ _ _ _ _ _ _ Aug 2006 Feb 2007 - Q *:' _nE�-- o _ _ —~Aug 2007 4 } + Feb 2008 Aug008 5 Q N ❑ _ 8 Location D µS/cm 0 25 50 75 100 125 150 175 200 225 250 0 ■ o Feb 2004 - 4 Feb2005 2 —Aug 2005 _Q_ __ __ _--_ _ _ _ _ F.b2006 3 Aug 2006 E 4 L 5 Q N � 6 7 8 9 10 Location G µS/cm 0 26 50 75 1100 125 150 175 200 225 250 0 5 E t 10 n a� 15 20 Figure 3-3. Semi-annual specific conductance profiles in Mountain Island Lake, 2004 — 2008. 3-17 • o Feb 2004 ♦ �- Aug 2004 ♦ F.b2005 Aug 2005 Aug 2006 ♦ • Feb2007 Aug 2007 Qi ♦ + Feb 2008 1-� Aug 2008 a 4 a . _ Figure 3-3. Semi-annual specific conductance profiles in Mountain Island Lake, 2004 — 2008. 3-17 Location A pH SU 6 7 8 Location B pH SU 9 5 6 7 8 9 5 0 1 3 E 4 L 5 CL Q) Q 6 7 8 9 10 0- 1 2 3 E 4 t 5 a CU 0 6 7 8 9 .. ... .. ... .. ... ... ... .. ........... o Feb 2004 --_----_ _ _ _ _ _ _ f _ _ _ _ o 1- 21- -o-Aug 2004 d Feb2005 ♦ * =--�-Aug 20041 - - ---- - �Y- af- • Aug 2005 o Feb 200 e Feb 2005 _ • Feb 2007 A -a- Aug 2005 - - - « • t - a Feb2008 � Aug 2006 a Feb 2008 0 �- Au 208 POOP 070 •� �.� Aug 20081 -Aug 208 _'�.. _ d] 4• • Feb 2007 _ _ _ _ _ _ _ _ _ _ d 04• n. so Aug 2007 l ■ o + Feb 20081 t A 2008 -- - --- - - -- -- 9da y♦ _ d °la i 5 0 1 3 E 4 L 5 CL Q) Q 6 7 8 9 10 0- 1 2 3 E 4 t 5 a CU 0 6 7 8 9 .. ... .. ... .. ... ... ... .. ........... o Feb 2004 --_----_ _ _ _ _ _ _ f _ _ _ _ _ . Aug 2004 -o-Aug 2004 d Feb2005 ♦ * -o-Aug 2005 - - ---- - �Y- af- o Fe6200 Aug 2005 o Feb 200 Aug 2006 _ • Feb 2007 A -f-Aug 2007 - - - « • t - a Feb2008 � Aug 2006 -r Aug2008 0 �- Au 208 Location C pH SU 6 7 8 9 5 • o Feb 2004 _ _ _ _.� -_ _ _ _ • >=o— Aug 2004 e Feb 200$ - - - • —� Aug 2005 • o Feb200 ____________ ��• Aug 2006 - - - - - - I Feb 2007 1 207 a • Feb 2008 • O • • Aug206 - - - - - - - - -- - - _ Location E pH SU 3 E 4 L 5 CL 6 7 8 Location D pH SU 6 7 8 9 Location G pH SU 5 6 7 8 9 5 6 7 0 2 3 4 t 5 d Q 6 7 8 9 10 0 5 t 10 d 15 20 8 9 0 Feb2004 _--__ __ _ _ 1 ♦R - -o-Aug 2004 Imo --Aug 204 a Feb2005 - - ---- - �Y- af- o- Feb 2005 Aug 2005 o Feb 200 -___-----_-_ _ _ _ _ 6 _ _ - _ � Aug 206 A o Feb 200 _ • Feb 2007 �Aug207 � Aug 2006 Feb 208 ------------------------------------ �- Au 208 Location G pH SU 5 6 7 8 9 5 6 7 0 2 3 4 t 5 d Q 6 7 8 9 10 0 5 t 10 d 15 20 8 9 Figure 3-4. Semi-annual pH profiles in Mountain Island Lake, 2004 — 2008. 3-18 D �• 0 Feb2004 a • Imo --Aug 204 d ♦ o- Feb 2005 p -o-- Aug 2005 o Feb 200 _ m - � Aug 2006 P ♦ 4CO ■ Feb207 -Aug207 • Feb2008 POOP 070 •� -Aug 208 _'�.. _ d] 4• c0411 d 04• e0� 1 l ■ Figure 3-4. Semi-annual pH profiles in Mountain Island Lake, 2004 — 2008. 3-18 250 200 150 E Cn 100 50 O --- — --- — - - - -- ------ ---- IL& ---- ----- -- - 1 N (V N N N N N N N N O O m N O O No _ N N N N m N N O O A B c D E G Sample Dates / Location Figure 3-5. Distribution of Mountain Island Lake specific conductance. 5 N pN o _O gN N O O N O O O O O O O Q O $ O O O O G O o o � O O O N N N N N N In N A B C D E G Sample Dates / Location Figure 3-6. Distribution of Mountain Island Lake pH. 70 so 50 40 O U Lj 50= - i ! ! i i ! ! i- i i T i"' T= T i i i I 45 - r- r - � i r --�-� r F-- =r +-� - - a - r r- t - � + --� - r - _t=r -� _•_-, r 1 ! ! 1 ! ! f f ! ! � _ 35 t G I�=, __ gl I ! { l + I Jv l I G r I— i a r t �— f I f— 1 �� t——r T=r�� VI——c t ~ 25 - ---- f - - - ---- -- - -- - -- - -- --- - -------= Z'20 -1- ----- I -------I ------ T --- - - ------ -------1 ------4------ ------ -- -I ------ ------a-- ---- -----1------ 15 ----15 --f -- 1 + f - f- 1 1- t 7- f i I- � t- - , Y- -- f -- --f- - - - ----- 10=a ` IID Tja- T�jr �I 5 41i `Y 0 0 0 0 0 0 0 0 o o g o 0 A B C D E G Sample Dates / Location Figure 3-8 Distribution of Mountain Island Lake turbidity 80 T r i- i i l 70 r 1 r Fr - sA r r r r i so = ---- - ---=- - - = L----- -' y-- --- = --- --- -- 50 40 ! ! ! ------ . !------ r----- -*--- - t - - --------r- - - r ----r-- - -r- -- -r r -----T �- r- -- r - --*--- -r---- -- - -- -- r -- -� r --i 20 I I ----- ! -} i + -I-- ---I ! --4 J- -J T- I-- I------1 10 o - - M M 0 A B c D E, G Sample Dates / Location Figure 3-9 Distribution of Mountain island Lake total suspended solids concentrations. -T= i- E, -' - 01 I 1 —; t t 1 1 t r I t t jjj I �il I ii I SI I I ISI I I I—� i t iii I I Ill O O O 00 o O O o O O O N o O o N O N o O N N O O A B Sample Dates / Location Figure 3-10 Distribution of Mountain Island Lake ammonia nitrogen concentrations 3-20 r i I i i i g i I i i I i i i I i i i i i i 2,5 --- ---------+ ------ - a 1 1- I i I I i I 2,0 i i { !- i- 1 4 r I -_ ! 1 � 4 =_-_= t- s l 1 + 4 =l l-- -��.- --=..i a ILL I I I I I I I i I I i I I I I ! I I 1 5 - - r--- -�- " - r ---- r r - r- -- r - -r ---+-- ---t- - r ----r- r -- --r- --- r- --- +---- -r- -- -- ---'-r -- � ` r------ r- -r -i 1 1 1 1 1 1 1 1 1 1 I I I I I 1 O ...... i i I t i t i Y 1- I I I! I I I�I O 5 --'-----' - 00 A B G D E G Sample Dates / Location Figure 3-12 Distribution of Mountain Island Lake total nitrogen concentrations 007 O 06�- _ i 7 1 1 1 �' - � i ..•`�- `�1 - I I i i i i - i i - 4005 -----+---- - -- --- ----- - -- i -- i ` i ( � O 04 -� -----� -- --�------�----- T - =T- --- T-- ---I- ---{-------{--- --T -----'�------T-- -T- --- �- ' -;-- -- }- --------- T-- --- I--` f --- --- ---- -- ----i 003 E f =- 4{+ I +- -{ T i f f j` 0., - T- --A rf 002 001 - �-� r 000 o O Q O o O O o o O o Q O o O N O o N N O O O o o N A, B C D E, G Sample Dates / Location Figure 3-13 Distribution of Mountain Island Lake soluble orthophosphate concentrations 3-21 T I T— 1 �.,6 q ---- - ---- - -- --- ---- ------ i - I j ----------'-- - - - --- ---- - -------=-- --- i f- t- I i �- � � I - -- ------ t-' r- I I I I - -- --- -- i t- i � j---� 1 2 � ----`------ -- ----- - ------ -- --,------- ---; -- ; 0 8 -fI ----- - 1------- t ----- I� If - 1- 1i - ti fI �- I i i - i i i i 06 I 1---- 0,4 02�}}r I I i t t T �7r-rS}t�+�I i i i I I i I j I Y t' 1 1 i 7 I Y I I I i I T i i I O.0 o N o o O o O O o 0 0 0 A B C D E G Sample Dates / Location Figure 3-11 Distribution of Mountain Island Lake nitrate+nitrite nitrogen concentrations r i I i i i g i I i i I i i i I i i i i i i 2,5 --- ---------+ ------ - a 1 1- I i I I i I 2,0 i i { !- i- 1 4 r I -_ ! 1 � 4 =_-_= t- s l 1 + 4 =l l-- -��.- --=..i a ILL I I I I I I I i I I i I I I I ! I I 1 5 - - r--- -�- " - r ---- r r - r- -- r - -r ---+-- ---t- - r ----r- r -- --r- --- r- --- +---- -r- -- -- ---'-r -- � ` r------ r- -r -i 1 1 1 1 1 1 1 1 1 1 I I I I I 1 O ...... i i I t i t i Y 1- I I I! I I I�I O 5 --'-----' - 00 A B G D E G Sample Dates / Location Figure 3-12 Distribution of Mountain Island Lake total nitrogen concentrations 007 O 06�- _ i 7 1 1 1 �' - � i ..•`�- `�1 - I I i i i i - i i - 4005 -----+---- - -- --- ----- - -- i -- i ` i ( � O 04 -� -----� -- --�------�----- T - =T- --- T-- ---I- ---{-------{--- --T -----'�------T-- -T- --- �- ' -;-- -- }- --------- T-- --- I--` f --- --- ---- -- ----i 003 E f =- 4{+ I +- -{ T i f f j` 0., - T- --A rf 002 001 - �-� r 000 o O Q O o O O o o O o Q O o O N O o N N O O O o o N A, B C D E, G Sample Dates / Location Figure 3-13 Distribution of Mountain Island Lake soluble orthophosphate concentrations 3-21 suoijualuoouoo wntsou5m iju-1 puelsl umjunolN jo uoljnqujsi4 91-£ o.mi?iA uolleoo-1 / salea aldwes J 3 O 9 v 0 0 0 o N N N N 00 o N 00 0 o O o iN O o N O O o O 00 i i i I I i I I 1_ - ' i i , } o L , � I i 1 } -- 1 I 1 o z - -- --- ------ I i P 'r =i 1 O E I I I i i I I I i I i i I i I I T I I I I suo►lvaluaouoo wntol-eo olL,-1 puL,lsl utL,IunoINjo uoiinglalsi4 'SI-£' oanf?i3 uolleool / solea aldwes `J a o e v N o O o 0 0 o A o 0 0 0 0 0 O o N o o O o O o 0 o O w W i- i w w - W i m i W - I w W W'- W 0 f z i I t I 1 I i I I i I I I i i I t I I i i i i i i i I I i i I I I i - A •suoqualuaouoo snaogdsogd lL,Iol oNt,-I puL,lsl u►ulunoIN jo uoiingialsi4' tl-£ aanolA o N o m W o �p w 3 O o N O o m W �O w uolleool / saleQ O 0 0 N o m W <D W aldwes 0 0 0 8 0 0 Co w tO 0 0 0 m 9 0 cp v o N 0 0 m '"• w ,� I i T, --- Egi� 000 zoo -1 F I 1900 11 i I I I I I I i I I I -- __1_-- --� ----- i - ' i -- _ - - - --� - _ - -- I j i i - - ---- , - f - f - 20 O J. a + -+ OLO --r--- --�----- I I I I I I I I I I I! I I! I r---- --- r-- - r- -- --r - -T----r- -- -, -- --r--- -r- -r----- r - ---r----- r--r-----r-- T--- -r--- - --- r -----i - - z L O I i i I 12 --T -- - --- --- 7 -1 ----- -- T- -- - ----- -- ------- ---- -- - 10 1 1 T 'T 1 t -1 i f - t 4 2 0 e 93 M 8 8 - -- - - - - - - - - -- - - A B C D E G Sample Dates / Location Figure 3-17 Distribution Of Mountain Island Lake sodium concentrations. 3 5 - -- ---- ----- - ------- 3 0-' - ------- - - -- --- ----------- 1----T -T- 2,5 20 E '11 5 J 1 0 ------ -------- ----------- -- 05 t------I-------� ------+-4- -- ---i------{------=-------t------ 4 ------- --------- --- 4--- --a- - ---- ! ------ ------t ------- -- ----i------a 0,0 iE3 2 M M 2 0 P2 C� 93 8 A B c D E G Sample [Dates Location Figure, 3-18 Distribution of Mountain Island Lake potassium concentrations 16 - - --------- - -- ------T--- --T --T -----T---- --- --- -------------- -- ---- ---T-------r- ----T -T-- -7 ---- T ----- 7 ---- -------------- ----- ------ I 14 - I I I I I ! I 4-- ----- - ------- - ---- --- - - - j ------ ----------- -t-------I----- 1 4 -----a 12 +1 10 8 - - 1 T J -1 I j- -IT1 1 T i i I iE i i i { i 'i l6 4 2 - ------- F- f -- ---- I -- ---- f i --- T -- ---- I ---f 1 - ----- T T ------ I I- ------ i 0 - 2 I 8 8 41 8 8 M 4 M C 8 ; 8 93 4 M 8 a ;i - A B C D E 0 Sample [Dates Location Figure') -19. Distribution of Mountain Island Lake chloride concentrations. 3-23 J7Z-£ •suoijualuaouoo osouvOumi axnj putisl uiulunoWjo uoijngiijstQ -ZZ-£ aangi j uop0007 / sa;ea aldweg �J 3 O J 9 V N N N N N NN N N N N A � A O N O N N O N N O N at N N O N N O N N o O o 0 o m O m LOO O L •suoiluiluoouoo uoai a3iu-1punisl u►t,junoWjo uoiingijlsiQ - j Z-£ oin2i j uo!;eoo7 / sa3eQ oldweg 0 N N F IP O O O 3 o N N O O O N O � N N O N O 9 laf N N N fop d N za O O O 9 r OL ZL bL 9L IT4 --- - - - i— - ------------------ - - ----- ------- ------- ---- •suoijuiluaouoo alnjins axu7 puuisl u►njunoWjo uoiingialsiQ -OZ-£ wn2i3 uoi;eoo-7 / sa;ep 491dweg �J N N N N O 3 N N zb O 0 N N o_ oO N O __ N N O 9 N NN O o .P N O V N g O a N N O 9 r OL ZL bL 9L O Z b 9 r OL ZL bL 9L sZ_, , suoiJUJIu'aouoo o[uas'Jt, oy�-1 purjsl u►BjunoAjo uo►jnq'J1s►Q SZ -c oanSiA uol;eool sa;ea aldwes o a a o a v Z8 8 o N N 0 0 0 0 0 0 O suoilei uoouoo Logts oyu­j puL,lsi mrjunciN jo uminquis►q t,'Z-c oJnOi j uol;eoo- sa;eCl oldweg 0 3 a o e v .s N 0 0 0 0 0 0 0 0 0 0 0 0 �g Z8 t3 O r -- +- r--- I- t �--+--N -1 1 --- t i t r _1 � I '! I I I I i i I I I I I i i i I i - I I r - 1 II- I b i 1 i I 9 I 1 i ' 1 I r- - t- --' i 'i •- -� - - -1 ` -- t t it - -', - 'i - t- i--- t 1 + suoiluxauoouoo umui,umlL,ayu�I puul,sl u►uluno`jN, jQ uotingrJlsi,a cZ-c aan�i � u011e007 / saiea eldwes 0 3 a o s v mow 000 I i i i I i i I i I i i I i i i i i I i i I I iT A I 'I I i i I I i i i A I I I i O Z O i i i i I I I i I I I I i I i 1 i I 'I i i I i I I I i i ' I ' r- ---t- - t-- --+ t + - r t-- --' r -+-- -+' --'- t t - r t ---t- tom- -t -t - --' t r-- - O E p 0 as 0170 I I I l -'--- ---i I I------ - I {IL ----- i os o I ip i i r i i I i I I i i I I I I I I i i090 I 1 ' i I i 1 i h I I I I I 1 I f I i I 9 �n I I i 1 ' I I 8 i I t I + r n � i J i ' i I I I t ��Is�a3a,��a r� r r q i_�y -� r r i f -' OL Z L suoilei uoouoo Logts oyu­j puL,lsi mrjunciN jo uminquis►q t,'Z-c oJnOi j uol;eoo- sa;eCl oldweg 0 3 a o e v .s N 0 0 0 0 0 0 0 0 0 0 0 0 �g Z8 t3 O r -- +- r--- I- t �--+--N -1 1 --- t i t r _1 � I '! I I I I i i I I I I I i i i I i - I I r - 1 II- I b i 1 i I 9 I 1 i ' 1 I r- - t- --' i 'i •- -� - - -1 ` -- t t it - -', - 'i - t- i--- t 1 + suoiluxauoouoo umui,umlL,ayu�I puul,sl u►uluno`jN, jQ uotingrJlsi,a cZ-c aan�i � u011e007 / saiea eldwes 0 3 a o s v mow 000 I i i i I i i I i I i i I i i i i i I i i I I iT A I 'I I i i I I i i i A I I I i O Z O i i i i I I I i I I I I i I i 1 i I 'I i i I i I I I i i ' I ' r- ---t- - t-- --+ t + - r t-- --' r -+-- -+' --'- t t - r t ---t- tom- -t -t - --' t r-- - O E p 0 as 0170 I I I l -'--- ---i I I------ - I {IL ----- i os o I ip i i r i i I i I I i i I I I I I I i i090 0.16 , r r I I t + I1 O, 12 + ! + G 0,10 1 -+- i =� a + t t �_T" -� i 006 1 7 1 1 IT 1 r a 7 "7' �< I 0,04 ------ ----- -{-- - i 4-----+ --- k' — -- - -k ------ - i i i I i -- - I- 00�i--'4'I� II T`i `1 1 —t r O 02 r- r r- ----r- -r r-- r r r- -r- r r r r r - I 1 i i I I I o 0 0 0 00 0 0 0 0 0 o 0 0 0 0 0 0 A B C D EI G Sample Dates / Location Figure 3-26 Distribution of Mountain Island ,Lake barium concentrations -'------ --- - ----T -------- •- 10 i ry I i i i i i 1 i �I E I 1 i i i I I I i I I 8 m - 1 j +..... .-- -- i.w= i f - w —+ j i i i i i i i i I I i i i I i i 4 II i I I i i i I i i I I I I 1 i IiiII --"-J--I+iii1I +IIIII --{--]--Y5,,I -- --- ii+i` ----- 11+I1 -- - 0I4P2 T= r T zIr 1iIIIII I I i I I I I I I i i i i I i I I i i i i I I I I I i i I i i I 0 41 o O o 0 0 0 0 0 A B CD E G Sample Dates / Location Figure 3-27. Distribution of Mountain Island Lake copper concentrations 12 •- --r----- -- --- _ ------•----- - --- -- I r - --r -- - r -- --I Oma 6 4 k � k JH --L- 1 = t { h i I _.• qs .+- I , I I , 4 t F ---'-------I i 1 ' I r---- -- I -- i-----+-- I i 2 I _I _ O -- o O93 93 o 0 o O o 0 0 o M S3 O c N o O O O O O o O o V(T O O o o O O O O O O O A B C D E G Sample Dates / Location Figure 3-28. Distribution of Mountain [bland Lake soluble copper concentrations ,3-26 LZ-£ suoijaaluaouoo ouiz a3juZ puuisl uiulunoWjo uoijngpjstQ '0£-£ amid uoll0007 / sa;ep aldwes �J N N N N O 3 N N N N > O a N N N N O o N N N o O 9 N NO cD O O b N N N N O L ___-_ –T t- T - - - – — _—_ --- _— ——_ ____– -- _-- _— ___-__ –____ _–___ ____– –____ – _______ ------ ----- - _____ -_ --------- _I_----L__-;-____�_- __-- --_-_---- suoijuil aouoo ,Cano.iauz alu-1 puujsl uitjunoWjo uoijngiajsiQ -6Z-£ oin2i3 uoi;eaol / sa;eQ aldwes `J O OatN N o O 3 N N O O N N N N O No N m N N O 9 O O N O O d N N N O L ___-_ 1.000 0 01-000 001.o ofC 000 L 0 0000 L 25,000 a ns 20,000 E a� a' 15,000 CO U Cn 0 a 10,000 az 0 n3 5,000 a) WE cl Discharge Flow ■ As Loading -------------- ------------------ ---- ----------------------- ---- ---------------------------------------------- ---- --------- -- -- -- - -- - -- -- - -- - - -- -- - 1,800 1,600 1,400 D N 1,200 r- 0 0 m 1,000 0 CO 800 Cfl a 600 m 400 200 1W N Cl) � LO CO 1- O M O N M -'r U) CO r— CO M O M O O O W O O O O O O O O O O O 07 D7 O O O O O O O O O O O O O O N N N N N N N N Figure 3-31. Average annual RSS ash basin discharge and arsenic loading to Mountain Island Lake, 1992 — 2008. 25.000 a CU a 20,000 .E a� a' 15,000 10,000 a CO 0 C CO 5,000 m C m 50 CD 40 r- 0 0 v 0- 30 (D 0_ 20 10 :7 N CO IT to (0 r— 00 O ON M I- LO M r— CO O O O O M M O O O O O O O O O O O O Q) Q7 O d1 O O O O O O O O O O O O r c- r- c- N N N N N N N N N Figure 3-32. Average annual RSS ash basin discharge and selenium loading to Mountain Island Lake, 1992 — 2008. 3-28 11 Discharge Flow -------------------------- ------------ ---------------- ----------------------- --------------------------------- -- -- -- -- -- -- -- - -- -- -- -- -- -- ■ Se Loading ----------------------------- - -- -- -- -- - - -- -- -- -- -- -- -- -- ---------- ------------ -- -- - -- -- -- -- -- ---- -- -- -- --------- - - -- m 50 CD 40 r- 0 0 v 0- 30 (D 0_ 20 10 :7 N CO IT to (0 r— 00 O ON M I- LO M r— CO O O O O M M O O O O O O O O O O O O Q) Q7 O d1 O O O O O O O O O O O O r c- r- c- N N N N N N N N N Figure 3-32. Average annual RSS ash basin discharge and selenium loading to Mountain Island Lake, 1992 — 2008. 3-28 100-T-- -� - �-�- -�- - �- r ---r--- T—T- I I(( I l l l j l l l l l l(( l l l l 90 ---t - ---G- --i 4 - J t 80 4 i�- !'! ....I -' _ i iji iIjiI iI iI Ii I_I_7o i ! `• `: ! I i! j L! 60 60 t ---C- 50 -L-!- i- --' --1-1-1- ! -i- 1-1 1—� --1- -�-1- �-- W j 40 -t- -- +-t- I-- fi t- j -- t- --r _ 1 t-�- - 30 ( I I I I -- C- -I :- + - , -- t- 4- + + 10 l !EjjJJ i i i� i� 0 aoCDo v rn v o ao rn o v rn v o o rn o v rn v o0 0o rn o v rn v ao CO CO CD rn o o ao 0o rn rn rn o o ao ao rn rn rn o 0 00 0o rn rn rn o 0 N N N N N N N N A(4 8 km US) B (0 5 km US) C (0 6 km DS) G (10 1 km DS) Sample Dates / Location Figure 3-33. Distribution of arsenic concentrations in sediment fines sampled from Mountain Island Lake upstream (US) and downstream (DS) of the RSS ash basin outfall. 40 T- i ! �-_i T.T - ; j - i -i 1 1 1-; 1-! I j i i i ii l i i i i i l l i i i i 35 - + -+ -�— r - r--- + - r—+ + -- a - ) -4 35 + --+ ------- - + - j i i E l i l i i i i i i i l E i i i i i i i i 30 i --t-4 25�'` } :S 20 E 15 u� 10 -t -F - ; -t-- +--t- � 5 i i i i i i I i 0 i i i m o o m m o m co CD rn o o m ao G)rn rn o 0 0o ao rn rn rn o o ao ao rn rn rn o 0 2 2 N N m n N N T T T T N N 62 Q2 T N N A(4 8 km US) B (0 5 km US) C (0 6 km DS) G (10 1 km DS) Sample Dates / Location Figure 3-34. Distribution of selenium concentrations in sediment fines sampled from Mountain Island Lake upstream (US) and downstream (DS) of the RSS ash basin outfall. 3-29 CHAPTER 4 MACROINVERTEBRATES MATERIALS AND METHODS Benthic macroinvertebrate sampling was conducted annually in July 2004 — 2008 as part of the continuing monitoring program for Riverbend Steam Station (RSS). Samples were collected from three locations in Mountain Island Lake: Location B, approximately 8.5 km upstream of RSS, Location F adjacent to the RSS discharge, and Location G approximately 3 km downstream of the RSS discharge near Mountain Island Hydroelectric Station (Table 1-1 and Figure 1-1). Note that in past reports (macroinvertebrate chapter only), Location B (277.6) was incorrectly referred to as Location C (277.5) (Duke Power 2001 and 2004). A ponar dredge was used to collect five sample replicates at each location. Samples were collected at depths ranging from 2 — 3 in to bracket the depth of peak benthic abundance (Brinkhurst 1974). Samples were washed through a 500-µm mesh sieve and individually preserved with 70% ethanol containing rose bengal stain. Organisms were sorted from the debris in the laboratory and identified to the lowest practicable taxon. Oligochaeta were not speciated until after 2001, when they were also identified to species, if possible (Tables 4-1 through 4-3). Macroinvertebrate densities were calculated and recorded. The assessment of the balanced and indigenous nature of the benthic community was determined by comparing macroinvertebrate densities and taxa abundance at locations upstream, downstream, and adjacent to the RSS discharge. RESULTS AND DISCUSSION Substrate The substrate at Locations B (upstream of RSS) and F (RSS discharge) was usually similar, being composed primarily of silt. The substrate at Location G (downstream of RSS) was composed mainly of gravel and sand. Other substrate components observed in the samples included: Naas (aquatic macrophyte), clay, clam shells, and organic matter. These same components were recorded from location substrates in 2000 — 2003 (Duke Power 2004). 4-1 Density During 2004 and 2005, densities at Location B were the lowest recorded among locations sampled, while the density at this Location in 2006 was the highest recorded among locations that year, as well as the highest recorded for the entire period of 2000 — 2008 (Table 4-1, Figure 4-1). In 2007, the density at Location B declined once again to the lowest among locations and then showed an increase in 2008 to the intermediate density among locations. Densities at Location F showed a sharp decline from the highest spatial value in 2004 to a comparatively low value in 2005. Densities at Location F increased in 2006 and then increased again in 2007 to become the highest among locations once again. The density declined once more in 2008 to the lowest value among locations that year (Table 4-2, Figure 4-1). At Location G, a slight decline occurred from 2004 to 2005, however, the value at Location G was the highest among locations in 2005. Location G showed a dramatic increase in density in 2006 to the highest value recorded from this location during 2000 — 2008. Densities at this location declined in 2007 and then increased to the highest among locations in 2008 (Table 4-3, Figure 4-1). Overall, the long-term average densities from locations sampled from 2004 — 2008 were higher than those of the previous report period of 2000 — 2003 (Tables 4-1 through 4-3, Figure 4-1). Taxa Abundance The number of taxa recorded from macroinvertebrate samples is typically a good indicator of overall diversity and the presence of balanced indigenous populations. Taxa abundance showed a slight decrease at all locations between 2004 and 2005, followed by a substantial increase in taxa numbers in 2006 (Tables 4-1 through 4-3, Figure 4-2). Location G generally demonstrated the highest taxa numbers among all locations (Table 4-2, Figure 4-2). During 2004 and 2005, taxa abundance was lowest at Location B, and, in 2006, Locations B and G increased to 36 and 35 taxa, respectively, while 32 taxa were recorded from Location F (Tables 4-1 through 4-3, Figure 4-2). During 2007, numbers of taxa declined slightly at Locations B and F (31 taxa each), with a corresponding increase at Location G to 38 taxa, the highest number recorded at this location during 2004 — 2008. Location B experienced a substantial increase in taxa from 2007 to 2008, from 31 to 43 taxa, the highest number recorded from Location B or any other location during 2000 — 2008. Locations F and G showed declines in taxa numbers over the same period, with the lowest number of taxa observed at Location F. Long-term average taxa numbers at all locations were higher in 2004 — 2008 than during 2000 — 2003 (Tables 4-1 through 4-3, Figure 4-2). 4-2 Samples were comprised primarily of Oligochaeta, Diptera, and Corbicula. At Location B, Oligochaeta abundance showed a continuous decline from 2001 — 2005, when less than 50 individuals were observed (Figure 4-3). The density of oligochaetes increased dramatically at Location B in 2006 and then declined once more in 2007, followed by an increase in 2008. Oligochaetes dominated densities at Location B in 2007 and 2008. Oligochaetes demonstrated exceptionally low densities at Location F from 2003 — 2005, then increased through 2008 to become the dominant group at this location (Figure 4-4). At Location F, oligochaetes demonstrated a similar temporal trend as that observed at Location G, with increasing values from 2004 — 2008 when they also became the dominant forms at all three locations (Figures 4-4 and 4-5). During 2004 — 2008, Diptera typically showed low relative abundances at Location B, while they were most often the dominant forms at Location G (Figures 4-3 through 4-5). At Location F, dipterans were typically more abundant than oligochaetes, but were most often less numerous than Corbicula (Figure 4-4). Dipteran densities varied at Mountain Island Lake locations from year to year, showing no consistent long-term patterns. Corbicula densities and relative abundances were typically lowest at Location G, while the highest densities were most often recorded from Location F (Figures 4-4 and 4-5). At Location B, Corbicula densities were low during all but 2006 when Corbicula constituted the largest proportion of macroinvertebrates recorded (Figure 4-3). No consistent year-to-year trends were observed among Corbicula populations at Mountain Island Lake locations during 2004 — 2008. CONCLUSIONS Corbicula densities and relative abundances were typically lowest at Location G, while the highest densities were most often recorded from Location F (Figures 4-4 and 4-5). At Location B, Corbicula densities were low during all but 2006 when Corbicula constituted the largest proportion of macroinvertebrates recorded (Figure 4-3). No consistent year-to-year trends were observed among Corbicula populations at Mountain Island Lake locations during 2004 — 2008. 4-3 Table 4-1. Densities (number/m2) of macroinvertebrates collected annually from Location B (upstream of RSS) from 2000 — 2008. Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Annelida Hirudinea Pharyngobdellida Erpobdellidae Erpobdella spp 9 Rhynchobdellida Glossiphonudae Helobdella spp 26 60 17 26 Oligochaeta 887 1,292 Haplotaxida Naididae 26 34 9 17 Arcteonats lomondi 17 Dero obtusa 17 Dero vaga 52 Homochaeta naidma 9 26 Naffs communis 34 9 Na►s vanabdis 585 77 267 Ophidonais serpentia 60 Pristina acuminata 9 Pristina longisoma 9 Pristina sima 52 69 Prisbnella osborrn 9 17 Stylana lacustrns 9 9 26 Tubificidae 499 809 448 86 43 2,462 1,128 1,791 Aulodrnlus limnobius 43 121 Aulodrdus pigue6 26 17 17 Branchirua sowerbyi 43 680 353 405 Limnodrnlus spp 138 Limnodrdus hoffineisterei 17 60 121 9 34 Tubifex tubifex 69 Polychaeta Sabellida Sabellidae Manayunkia speciosa 34 241 9 86 284 17 Arthropoda Acan 17 Crustacea Amphipoda Talitndae Hyalella azteca 878 344 9 Insecta 4-4 Table 4-1. (Continued) Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Coleoptera Elmidae Promores►a spp. 9 Diptera Ceratopogonidae Alluaudomyia spp 34 Palpompa-Bezzia complex 86 129 17 17 34 482 95 215 Chaobondae Chaoborus spp. 9 Chironomidae-Chironommae Axarus spp 9 112 207 17 Ch►ronomus spp 9 Cladopelma spp 17 Cladotanytarsus spp 9 103 9 Cryptoch►ronmus spp 17 9 43 52 112 77 Cryptotendipes spp 26 146 9 60 9 17 129 189 121 Cryptotendipes emorsus 9 Dem►cryptoch►ronomus cuneatus 9 D►crotendipes spp 9 D►crotendipes neomodestus 9 9 Harn►sch►a spp 52 26 Microch►ronomus spp 9 N►lothauma spp 26 34 Paracladopelma spp. 60 Paralauterborn►ella spp. 9 Paratanytarsus spp 9 Polypeddum spp 9 Polypeddum flavum 9 Polypeddum halterale gp 9 9 9 34 17 9 17 Polypeddum scalaenum 60 Pseudoch►ronomus spp 52 26 9 26 26 293 258 9 129 Saethena spp 60 Stempelhna spp 9 Stenoch►ronomus spp 9 Stictoch►ronomus spp 26 52 60 26 112 112 St►ctoch►ronomus cafiramus 26 86 Tanytarsus spp 138 17 17 26 Chironomidae-Orthocladunae Cncotopus b►c►nctus 9 Cncotopus pohtus 17 52 Parakieffenella spp 9 17 Chironomidae-Tanypodinae I Ej 4-5 Table 4-1. (Continued) Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Ablabesmpa spp 34 26 301 Ablabesmpa annulata 17 9 17 9 Ablabesmpa janta 26 26 9 9 Ablabesmyia mallochi 34 164 26 9 17 17 43 Cbnotanypus spp. 17 17 17 Cbnotanypus pmguis 60 Coelotanypus spp. 34 103 60 9 52 Djalmabat►sta pulchra 9 9 60 9 Labrundrnia spp 9 Procladius spp 155 258 17 60 138 Procladius bellus 34 Simuludae Simulium spp 9 Ephemeroptera Baetidae 9 9 Pseudocentrophlo►des spp 26 Caemdae Cams spp 9 Ephemendae Hexagenia spp 9 198 9 103 Odonata-Anisoptera Corduludae 9 Neurocordulta spp 17 17 Gomphidae 26 9 Gomphus spp 9 Dromogomphus spp 9 Odonata-Zygoptera Coenagnonidae 17 Argia spp 9 9 Ischnura spp 9 Trichoptera Hydroptilidae 9 Hydroptda spp 9 17 9 Oxyethira spp 9 Leptocendae Oecehs spp 26 17 52 9 52 Tnaenodes spp 9 Tnaenodes injusta 9 Polycentropodidae C rnellus fraternus 17 Polycentropus spp 17 9 9 9 Mollusca 4-6 Table 4-1. (Continued) Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Gastropoda Basommatophora Physidae Physella spp 9 Pulmonata Planorbidae Hehsoma spp. 43 Pelecypoda Veneroida Sphaemdae Pisidium spp. 1,705 Sphaenum spp 43 870 181 155 Heterodontida Corbiculidae Corbicula flummea 1,972 164 1,877 611 232 758 4,314 430 1,085 Nematoda 9 43 17 95 43 Nemertea Enopla Hoplonemertea Tetrastemmatidae Prostoma graecens 86 69 Platyhelminthes Turbellana Tncladida Plananidae Dugesia spp. 26 284 189 9 Total Density for Year 4,524 5,721 3,816 1,397 1,032 1,645 11,289 3,120 5,402 Total Taxa for Year 35 39 21 14 18 17 36 31 43 4-7 Table 4-2. Densities (number/m2) of macroinvertebrates collected annually from Location F (RSS discharge) from 2000 — 2008. Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Annelida Hirudinea Rhynchobdellida Glossiphomidae Helobdella spp 43 319 9 Oligochaeta 413 327 Haplotaxida Naididae 17 9 Bratislavia unidentata 9 Dero spp 17 Dero vaga 9 Nais communis 26 17 Naffs vanabdis 69 17 17 Pristina sima 9 9 Prishnella osborm 9 26 9 Stylana lacustris 43 Tubifiadae 276 818 52 232 121 189 Aulodnlus limnobius 17 Aulodnlus piguett 34 276 1,162 Branchirua sowerbyi 52 77 77 129 465 370 224 Polychaeta Sabellida Sabellidae Manayunk►a speciosa 17 155 52 34 Arthropoda Acan 9 Crustacea Amphipoda Talitndae Hyalella azteca 17 9 9 Decapoda Palaemonidae Palaemonetes sp 9 Diptera Ceratopogonidae Palpomyia-Bezzia complex 34 17 60 26 26 17 112 43 Chaobondae Chaoborus spp. 9 17 17 17 9 9 Chironomidae-Chironominae Axarus spp 17 43 60 Chironomus spp 26 17 17 4-8 Table 4-2. (Continued) Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Cladopelma spp 9 17 9 9 Cladotanytarsus spp 69 353 112 43 43 164 198 17 Cryptochironmus spp 9 43 26 9 112 43 Cryptotendipes spp 52 112 258 121 9 172 319 215 Demicryptochironomus cuneatus 9 9 Dicrotendipes spp 9 103 26 Dicrotendipes modestus 9 D►crotendlpes neomodestus 17 17 26 17 43 Harmsch►a spp 9 9 9 Microchironomus spp 17 9 Ndothauma spp. 17 34 9 Pagashella spp 60 43 172 52 327 86 17 Parachironomus spp 52 Paracladopelma spp 9 9 Paralauterborniella nigrohalterale 9 9 Polypeddum halterale gp 34 26 17 17 17 69 17 Pseudochironomus spp 17 60 258 69 164 198 353 17 Stempel6na spp 17 26 112 9 Stictoch►ronomus spp 17 26 Stictochironomus cafframus 164 353 17 43 26 Tanytarsus spp 9 9 26 60 17 17 34 52 Thienemann►ella spp 9 Chironomidae-Orthocladunae Cncotopus pohtus 9 Parakieffenella spp 9 Chi rono m idae-Tanypod inae Ablabesmpa spp 34 9 34 Ablabesmyo annulata 52 112 138 95 26 17 Ablabesmpajanta 26 9 43 26 Ablabesmyo mallochi 9 9 34 43 Coelotanypus spp 34 224 77 293 138 181 112 86 189 Djalmabatista pulchra 9 9 Proclad►us spp 155 155 69 568 129 60 95 310 293 Procladius bellus 77 Ephemeroptera Baetidae 9 Caenidae Cams spp 17 9 17 26 26 207 327 Ephemendae Hexagema spp 9 86 818 34 551 362 258 482 224 Megaloptera Sialidae Sialis spp 9 9 26 9 4-9 Table 4-2. (Continued) Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Odonata-Arnsoptera Gomphidae Gomphus spp. 9 Odonata-Zygoptera Coenagnomdae Ischnura spp 9 Trichoptera Hydroptdidae Orthotnch►a spp. 34 9 Leptocendae Oecetis spp 26 9 17 17 17 Tnaenodes spp 9 Polycentropodidae Cyrnellus fraternus 9 Polycentropus spp. 9 Mollusca Gastropoda Basommatophora Physidae Physella spp 34 Pelecypoda Heterodontida Corbiculidae Corbicula flummea 310 215 3,0571 629 3,272 430 1,748 3,057 594 Nematoda 26 26 189 198 26 Platyhelminthes Turbellaria Tricladida Planamdae Duges►a spp. 9 9 Total Density for Year 1,311 1,838 6,217 3,107 5,124 1,8194,867 6,436[!017 Total Taxa for Year 25 32 25 25 27 23 32 31 4-10 Table 4-3. Densities (number/m2) of macroinvertebrates collected annually from Location G (downstream of RSS) from 2000 — 2008. Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Annelida Hirudinea 34 77 Pharyngobdellida Erpobdellidae Erpobdella spp 17 Rhynchobdellida Glossiphonudae Helobdella spp 86 86 585 293 129 17 OI igochaeta 1,292 1,558 Branchiobdellida Branchiobdellidae 9 Haplotaxida Naididae 9 1 26 Allonais pectmata 26 Dero spp 26 Naffs communis 258 26 17 Naffs simplex 112 Nais vanabilis 172 34 827 Pristina longisoma 34 Pristina sima 34 34 103 Prishnella osbomt 17 17 17 Lumbnculida Lumbnculidae 9 52 Lumbrnculus spp 129 77 198 60 Stylodrdus hermgtanus 9 Tubificidae 1,093 1,042 181 17 422 672 336 1,205 Aulodnlus limnobius 26 Aulodnlus pigueti 17 17 Aulodrdus plunseta 9 Branchirua sowerbyi 69 34 801 577 706 301 293 499 Limnodrdus spp 17 Limnodrnlus hoffineisterei 17 17 Tubifex spp 9 Polychaeta Sabellida Sabellidae Manayunkia speciosa 319 362 Arthropoda Acan 9 Crustacea Amphipoda 4-11 Table 4-3. (Continued) Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Talitrndae Hyalella azteca 17 95 1,782 121 3,608 1,782 Diptera Ceratopogonidae Alluaudomyia spp. 9 Palpomyia-Bezzia complex 17 26 77 9 17 43 34 43 207 Chironomidae-Chironommae Axarus spp. 9 34 Chironomus spp 9 9 9 Cryptochironmus spp 9 26 103 86 121 Cryptotendipes spp 9 17 26 77 138 706 52 26 34 Dicrotendipes spp 697 Dicrotendipes neomodestus 17 9 189 293 69 224 Glyptotendipes spp 9 Hamischia spp 17 Microchironomus spp 9 Ndothauma spp 9 26 17 Pagashella spp 9 34 86 9 Parachironomus spp 9 9 Polypeddum halterale gp. 9 17 9 9 69 9 Polypeddum d6noense 9 Polypeddum scalaenum 17 26 9 422 Pseudochironomus spp 9 9 34 69 129 17 241 Stempellina spp 17 26 17 Stenochironomus spp 9 9 43 34 60 34 Stictochironomus spp 9 9 Stictoch►ronomus cafframus 17 Tanytarsus spp 26 95 34 258 86 146 Chironomidae-Orthocladnnae Cncotopus bicinctus 9 Cncotopus pohtus 9 17 9 26 Nanocladius spp 9 Orthocladius spp. 17 Parakieffernella spp. 17 17 26 17 Chironomidae-Tanypodinae Ablabesmyia spp. 129 86 43 34 103 Ablabesmyo annulata 43 26 9 Ablabesmyojanta 52 17 86 181 276 121 Ablabesmyta mallochi 138 26 9 60 60 34 86 112 224 Coelotanypus spp. 52 17 26 60 121 379 103 9 9 Djalmababsta pulchra 26 9 69 172 Procladius spp. 129 34 17 146 189 241 422 129 241 Procladius bellus 26 4-12 Table 4-3. (Continued) Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Ephemeroptera Baetidae 9 Caemdae Cams spp 9 9 60 34 1,300 Ephemendae Hexagen►a spp 9 34 9 181 Heptagemidae Stenacron mterpunctatum 34 9 52 Tricorythidae Trncorythodes spp 9 Odonata-Anisoptera Corduludae Neurocorduha spp 9 Gomphidae 9 9 Dromogomphus spp 9 Odonata-Zygoptera Coenagrionidae Argia spp 9 9 Trichoptera Hydroptdidae Hydroptda spp 17 9 Orthotnchra spp 9 9 9 Leptocendae Oecehs spp 9 9 9 17 9 17 69 Tnaenodes spp 9 17 Polycentropodidae Polycentropus spp 26 17 17 9 138 Coelenterata Hydroida Hydridae Hydra spp 9 Mollusca Gastropoda Basommatophora Physidae Physella spp 60 26 Limnophda Ancylidae Ferrrssia spp 9 Pelecypoda Heterodontida Corbiculidae Corbicula fluminea 1,920 3,685 784 964 603 482 775 387 422 4-13 Table 4-3. (Continued) Taxa 2000 2001 2002 2003 2004 2005 2006 2007 2008 Nematoda 69 26 52 26 241 60 Nemertea Enopla Hoplonemertea Tetrastemmatidae Prostoma graecens 172 121 Platyhelminthes Turbellana Tncladida Planamdae Dugesia spp 258 258 9 155 9 482 448 Total Density for Year 4,385 7,752 2,742 2,895 5,028 4,417 9,196 4,936 6,895 Total Taxa for Year 23 33 28 31 32 27 35 38 31 4-14 12,000 10,000 8,000 U) am c 0 6,000 Ca H 4,000 2,000 2000 2001 2002 i 2003 2004 ■B OF G 2005 2006 2007 2008 Figure 4-1. Density (number/M2) of macro invertebrates collected annually during 2000 — 2008 from Mountain Island Lake. 50 45 40 35 a m 30 0 m 25 x I� 20 H 15 10 5 0 2000 2001 2002 2003 2004 2005 2006 2007 2008 MB OF G Figure 4-2. Total number of macro invertebrate taxa collected annually during 2000 — 2008 from Mountain Island Lake. 4-15 10,000 9,000 8,000 7,000 6,000 m 5,000 a� O 4,000 3,000 2,000 1,000 0 Location B 2000 2001 2002 2003 2004 2005 2006 2007 2008 ® Oligochaeta ■ Diptera Corbicula Figure 4-3. Density (number/m2) of Oligochaeta, Diptera, and Corbacula collected annually during 2000 — 2008 from Location B in Mountain Island Lake. 6,000 5,000 4,000 m Z5 3,000 Q 2,000 1,000 0 Location F 2000 2001 2002 2003 2004 2005 2006 2007 2008 ■ Oligochaeta ■ Diptera o Corbacula Figure 4-4. Density (number/M2) of Oligochaeta, Diptera, and Corbacula collected annually during 2000 — 2008 from Location F in Mountain Island Lake. 4-16 8,000 7,000 6,000 5,000 N N c 4,000 m 3,000 2,000 1,000 0 Location G 2000 2001 2002 2003 2004 2005 2006 2007 2008 ■ Oligochaeta ■ Diptera Corbicula Figure 4-5. Density (number/m2) of Oligochaeta, Diptera, and Corblcula collected annually during 2000 — 2008 from Location G in Mountain Island Lake. 4-17 CHAPTER 5 FISH MATERIALS AND METHODS Winter and Summer Electrofishing Surveys Electrofishing surveys were conducted in Mountain Island Lake near Riverbend Steam Station (RSS) in January and July (2004 — 2008) uplake from the RSS intake (Location B), in the discharge canal (Location F), and downlake from the RSS discharge near Mountain Island Dam (Location G; Table 1-1 and Figure 1-1). The locations surveyed consisted of 100-m transects on both the left and right shorelines. Surface water temperature (°C) and dissolved oxygen (mg/L) were measured with a calibrated thermistor and dissolved oxygen probe, respectively, at each location. Stunned fish were collected by two netters, identified to species, and measured for total length (mm). Catch per unit effort (CPUE, number of fish/100 m) and the number of species were calculated for each sampling location. Spring Electrofishing Surveys Electrofishing surveys were conducted in Mountain Island Lake near RSS in April or May (2004 — 2008) uplake and downlake from the RSS discharge canal (Figure 1-1). The locations surveyed were identical to historical locations surveyed since 1993 and consisted of five 300-m shoreline transects at each location. Transects included habitats representative of those found in Mountain Island Lake. Shallow flats where the boat could not access within 3-4 in of the shoreline were excluded. All sampling was conducted during daylight, when water temperatures were expected to be between 15 and 20 °C. Stunned fish were collected by two netters and identified to species. Fish were enumerated and weighed in aggregate by taxon, except for largemouth and spotted bass, where total lengths (mm) and weights (g) were obtained for each individual collected. Surface water temperature (°C) was measured with a calibrated thermistor at each location. Catch per unit effort (CPUE, number of fish/1,500 m) and the number of species were calculated for each sampling location. 5-1 Fall Hydroacoustics and Purse Seine Surveys The abundance and distribution of pelagic forage fish in Mountain Island Lake were determined using mobile hydroacoustic (Brandt 1996) and purse seine (Hayes et al. 1996) techniques. An annual mobile hydroacoustic survey from the Mountain Island Dam to approximately Location C was conducted in mid-September to estimate forage fish populations (Figure 1-1). Hydroacoustic surveys employed multiplexing, side- and down - looking transducers to detect surface -oriented fish and deeper fish (from 2.0 in depth to the bottom), respectively. Both transducers were capable of determining target strength directly by measuring fish position relative to the acoustic axis. Purse seine surveys were also collected in mid-September from the forebay of the Mountain Island Dam (near Location G). The purse seine measured 118 x 9 in, with a mesh size of 4.8 mm. A subsample of forage fish collected from each area was used to estimate taxa composition and size distribution. Trace Element Analyses Selenium, arsenic, mercury, and zinc concentrations were measured in epaxial muscle tissue of common carp Cyprinus carpio, sunfish (typically redbreast sunfish Lepomis auritus), and bass (typically largemouth bass Micropterus salmoides) collected by electrofishing in July (2004 — 2008) at Locations A, B, C, and G (Figure 1-1). Muscle tissue was dissected from each of five fish from a particular taxon, where available, and trace element concentrations (µg/g, wet weight) were determined individually by neutron activation analysis at the Nuclear Services Laboratory at North Carolina State University, Raleigh, NC. Graphical methods were used to examine spatial and temporal trends in trace element concentrations. Balanced and Indigenous Assessment Annual sampling protocols are designed to assess the balanced and indigenous nature of the Mountain Island Lake fish community and provide information relative to potential RSS impacts. The assessment includes a comparison of CPUE and number of species data from locations uplake of, in the discharge canal of, and downlake of RSS during winter and summer electrofishing. These electrofishing surveys are part of the required RSS 316(a) annual monitoring program. Additionally, trace element accumulation in fish muscle is 5-2 evaluated in relation to the ash basin discharge. Spring electrofishing and hydroacoustic surveys near RSS are part of the Catawba-Wateree hydroelectric relicensing activities. RESULTS AND DISCUSSION Winter Electrofishing Surveys Winter electrofishing surveys from 2004 to 2008 produced 5,078 individuals comprising eight families, 21 species, and two hybrid centrarchid combinations (Table 5-1). The species composition for the combined winter electrofishing surveys was dominated by clupeids (47.5%), centrarchids (45.1%), and cyprinids (6.9%), with the remaining families representing less than 1.0% each. Data from winter 2000 — 2003 documented a lower percentage of clupeids (5.3%) and cyprinids (2.0%) and a higher percentage of centrarchids (91.4%; Table 5-1 and Duke Power 2004). Pollution tolerant species (i.e., longnose gar Lepisosteus osseus, grass carp Ctenopharyngodon Idella, common carp, golden shiner Notemigonus crysoleucas, white catfish Ameiurus catus, eastern mosquitofish Gambusia holbrooki, redbreast sunfish, and hybrid sunfish) represented 15.7% of the collected fish from winter 2004 — 2008. This percentage decreased from 41.6% of collected fish reported from winter 2000 — 2003. The CPUE and number of species at individual locations ranged from 41.5 (Location G in 2005) to 701 (Location B in 2006) fish/100 in and from four (Locations F and G in 2005) to 13 species (Location F in 2004), respectively (Figure 5-1, Appendix Tables A-1 to A-5). Spatial dissimilarities in CPUE (Location B in 2006 and Location F in 2007) and number of species (Location F in 2004 and 2008) were likely due to collecting schools of threadfin shad and discharge canal temperature, respectively. Winter electrofishing data are consistent with previous data (Duke Power 2001, 2004) and indicate no negative impact from RSS operations on Mountain Island Lake fish populations. Summer ElectrofishingSays Summer electrofishing surveys from 2004 to 2008 produced 4,054 individuals comprising seven families, 17 species, and two hybrid centrarchid combinations (Table 5-2). The species composition for the combined summer electrofishing surveys was dominated by centrarchids (66.1%), clupeids (27.5%), and cyprinids (6.2%), with the remaining families 5-3 representing less than 1.0% each. Data from summer 2000 to 2003 documented similar percentages of each: centrarchids (66.2%), clupeids (30.5%), and cyprinids (2.4%). Pollution tolerant species represented 30.3% of the collected fish during summer 2004 — 2008. This percentage decreased from 44.1% of collected fish reported from summer 2000 — 2003. The CPUE and number of species at individual locations ranged from 29.5 (Location G in 2006) to 288.0 (Location F in 2006) fish/100 in and from five to seven species (multiple locations for each; Figure 5-2, Appendix Tables A-6 to A-10). Spatial dissimilarities were due to collecting a school of threadfin shad (Location F in 2006) and a large centrarchid population (Location G in 2007 and 2008). Summer electrofishing data are consistent with previous data (Duke Power 2001, 2004) and indicate no negative impact from RSS operations on Mountain Island Lake fish populations. Suring Electrofishing Surveys Spring electrofishing surveys from 2004 to 2008 were conducted at water temperatures ranging from 12.7 to 20.8 °C. Fish numbers and biomass per 1,500 in ranged from 468 to 2,140 and from 45.7 to 131.2 kg, respectively, uplake of the RSS discharge and 706 to 1,389 and 38.4 to 81.6 kg, respectively, downlake from the RSS discharge (Table 5-3). The number of fish species ranged from 12 to 19 uplake and from 10 to 13 downlake of the RSS discharge. Previous reports documented that numbers of fish, biomass, and number of species collected uplake were generally greater than those from downlake locations (Duke Power Company 1994; Duke Power 2001, 2004). For 2004 — 2008 the numbers of fish and biomass varied between uplake and downlake, even though the number of species collected uplake was generally higher. Spring electrofishing surveys, from 2004 to 2008, were dominated by centrarchids (89.8%) and cyprinids (8.3%), with the remaining families representing less than 1.0% each (Table 5-4). Pollution tolerant species represented 28.3% (97.4% of that being redbreast sunfish) of the collected fish during spring 2004 — 2008. One new species, rainbow trout Oncorhynchus mykiss, was collected uplake in 2006. Most likely introduced by an angler, it would not be expected to survive the summer. Spring electrofishing survey data varied among years, similar to other Catawba River reservoirs. 5-4 Fall Hydroacoustics and Purse Seine Surveys Similar to Mountain Island Lake and other Catawba River reservoirs since 1994, hydroacoustics from 2004 to 2008 showed no temporal trend in annual forage fish population estimates (range = 601,00 — 3,597,000; 603 — 3,611 fish/ha) (Table 5-5). Purse seine surveys from 2004 to 2008 indicated a shift back to a threadfin shad Dorosoma petenense dominated (range = 80.2 — 99.8%) forage fish community. Alewife Alosa pseudoharengus, first detected in low numbers in 1999 (Duke Power 2001), have comprised as much as 83.1% (2003) of mid-September pelagic, forage fish community surveys, but have remained relatively low since 2004 (range = 0.2 —19.6%). Trace Element Analyses Trace element concentration means for common carp were typically comprised of less than five fish due to the difficulty of collecting common carp. The four complete common carp samples (out of 20 attempts; Location A from 2004 to 2006 and Location C in 2005) since 2004 had element concentration means mostly within the ranges of sunfish and bass. An individual sunfish from Location C in 2005 was a statistical outlier for all trace element concentrations and not included in analyses. All 2004 — 2008 trace element concentration means for sunfish and bass decreased at all locations relative to 1993 — 2003 and were within ranges reported from 1988 to 2003 (Harden and Reid 1991; Duke Power Company 1994; Duke Power 2001, 2004). Mean selenium concentrations in fish muscle tissue from complete samples collected from 2004 to 2008 ranged from 0.31 to 1.03 µg/g, wet weight (Figure 5-3). Mean selenium concentrations since 2004 from sunfish (range = 0.33 — 0.86 µg/g, mean = 0.53 µg/g) and bass (range = 0.31 — 1.03 µg/g, mean = 0.59 µg/g) were slightly greater than the average value of 0.50 µg/g measured in largemouth bass muscle tissue from 26 sites throughout the entire Catawba River basin during 1993 (Coughlan 1995). Location C, downstream from the ash basin discharge, had the highest mean selenium concentration for three out of the five years for bass and sunfish, although the years did not coincide between species. All measured values in common carp, sunfish, and largemouth bass were well below the North Carolina Department of Health and Human Services (NCDHHS) Action Level of 10.0 µg/g for the protection of human health (NCDHHS 2007). 5-5 Mean arsenic concentrations in fish muscle tissue from complete samples collected from 2004 to 2008 ranged from 0.03 to 0.17 µg/g, wet weight (Figure 5-4). Mean arsenic concentrations since 2004 from sunfish (range = 0.03 - 0.17 µg/g, mean = 0.09 µg/g) and bass (range = 0.04 - 0.17 µg/g, mean = 0.08 gg/g) were similar to the average value of 0.09 gg/g measured in largemouth bass muscle tissue from 26 sites throughout the entire Catawba River basin during 1993 (Coughlan 1995). All measured values in common carp, sunfish, and largemouth bass were well below the Division of Water Quality's tissue screening value of 1.20 µg/g (NCDENR 2006). Mean mercury concentrations in fish muscle tissue from complete samples collected from 2004 to 2008 ranged from 0.03 to 0.15 µg/g, wet weight (Figure 5-5). Mean mercury concentrations since 2004 from sunfish (range = 0.04 - 0.13 µg/g, mean = 0.07 µg/g) and bass (range = 0.06 - 0.15 µg/g, mean = 0.10 µg/g) were similar to the average value of 0.08 µg/g measured in largemouth bass muscle tissue from 26 sites throughout the entire Catawba River basin during 1993 (Coughlan 1995). All measured values in common carp, sunfish, and largemouth bass were well below the NCDHHS action level of 0.40 µg/g for the protection of human health (NCDHHS 2007). Mean zinc concentrations in fish muscle tissue from complete samples collected from 2004 to 2008 ranged from 2.39 to 8.31 µg/g, wet weight (Figure 5-6). Mean zinc concentrations since 2004 from sunfish (range = 2.39 - 5.54 µg/g, mean = 4.42 µg/g) and bass (range = 2.43 - 5.63 µg/g, mean = 3.81 µg/g) were below the average value of 6.71 µg/g measured in largemouth bass muscle tissue from 26 sites throughout the entire Catawba River basin during 1993 (Coughlan 1995). All measured values in common carp, sunfish, and largemouth bass were well below concentrations (16 to 82 µg/g) reported for several taxa of omnivorous freshwater fish from other areas in the United States (Moore and Ramamoorthy 1984). CONCLUSIONS A diverse fish community was present in the littoral portions of Mountain Island Lake around RSS from 2004 to 2008; winter and summer electrofishing surveys documented 21 species (and two hybrid combinations) and 17 species (and two hybrid combinations), respectively. Both surveys were numerically dominated by clupeids and centrarchids, with cyprinids also being of numerical importance. Spring electrofishing documented 26 species 5-6 (and one sunfish hybrid combination), dominated by centrarchids and cyprinids. Pollution tolerant species, mostly redbreast sunfish, comprised 15 — 30% of the Mountain Island Lake fish community. The assorted fish species typically found in Mountain Island Lake near RSS encompass a range of trophic groups including insectivores, omnivores, and piscivores. Forage fish population densities, as measured by hydroacoustics, were variable and ranged from 603 to 3,611 fish/ha from 2004 to 2008. These densities are comparable to those measured previously at Mountain Island Lake and to other Catawba River reservoirs of similar trophic status. The introduction of alewife, the inherent, temporal variability of clupeid populations, and the historical flux of Mountain Island Lake between oligotrophic and mesotrophic water quality classifications (Chapter 3) are all factors contributing to the unpredictable nature of forage fish densities. Trace element concentrations have been measured in Mountain Island Lake common carp, sunfish, and bass since 1988. Trace element concentrations from 2004 to 2008 remained well below values of regulatory concern, where such values exist. Past studies have indicated that a balanced indigenous fish community exists near the RSS (Duke Power Company 1994; Duke Power 2001, 2004). The present study adds more years of comparable data to help reinforce that conclusion. Based on the diversity and numbers of individuals in the Mountain Island Lake littoral fish community during winter, spring, and summer; the range of trophic groups represented; the lack of accumulation of selenium, arsenic, mercury, or zinc in fish flesh; and the regular availability of forage fish to limnetic predators, it is concluded that the operation of RSS has not impaired the Mountain Island Lake fish community. 5-7 Table 5-1. Number of individuals and number of species in winter electrofishing surveys from three locations (B, F, and G) in Mountain Island Lake, 1994 — 1999, 2000 — 2003, and 2004 — 2008. Winter 1994 - 1999 Winter 2000 - 2003 Winter 2004 - 2008 Scientific Name Common Name No % No % No % Lepisosteldae Lepisosteus osseus Longnose gar 3 011% 8 014% 9 018% Clupeidae Dorosoma cepedianum Gizzard shad 66 234% 12 021% 8 016% Dorosoma petenense Threadfin shad 285 507% 2,402 4730% Cyprinidae Ctenopharyngodon Idella Grass carp 1 004% 1 002% Cypnnella chlonstia Greenfin shiner 1 002% Cypnnella nivea Whdefin shiner 146 518% 29 052% 285 5 61 % Cypnnus carpio Common carp 76 270% 24 043% 3 006% Notemigonus crysoleucas Golden shiner 3 011% 4 007% 5 010% Notropis hudsonius Spottad shiner 6 021% 55 098% 7 014% Notropis procne Sw allow tad shiner 49 096% Catostom idae Moxostoma collapsum Notchlip redhorse 1 004% Ictaluridae Ameiurus brunneus Snail bullhead 1 002% Ictalurus punctatus Channel catfish 6 0 21 % 18 032% 3 006% Poeciliidae Gambusre holbrooki Eastern mosquitofish 2 007% 7 012% 3 006% Moronidae Morone chrysops White bass 6 0 21 % 2 004% Morone saxablis Striped bass 3 0 11 % 11 020% 10 020% Centrarchidae Lepomis auntus Redbreast sunfish 359 1274% 2,290 4077% 764 1505% Lepomis gibbosus PUmpkmseed 46 163% 2 004% 4 008% Lepomis gulosus Warmouth 24 085% 37 066% 10 020% Lepomis hybrid Hybrid sunfish 10 035% 3 005% 9 018% Lepomis macrochirus Bluegdl 875 3106% 2138 3806% 1,136 2237% Lepomis microlophus Redear sunfish 237 8 41 % 333 593% 164 323% Micropterus punctulatus Spotted bass 3 006% Micropterus salmoides Largemouth bass 905 3213% 313 557% 185 364% Micropterus hybrid Hybrid black bass 5 010% Pomoxis annulans White crappie 1 004% Pomoxis mgromaculatus Black crappie 37 1 31% 19 034% 11 022% Percidae Etheostoma fustforme Sw amp darter 1 004% Etheostoma olmstedi Tessellated darter 2 007% 10 018% Perca flavescens Yellow perch 1 004% 15 027% 2 004% Total No. Individuals 2,817 100.00% 5,617 100.00% 5,078 100.00% Total No. Species 23 22 21 5-8 Table 5-2. Number of individuals and number of species in summer electrofishing surveys from three locations (B, F, and G) in Mountain Island Lake, 1991 —1993, 1994 — 1999, 2000 — 2003, and 2004 — 2008. 5-9 Sumner 1991 - 1993 Sumner 1994 - 1999 Sumner 2000 - 2003 Sumner 2004 - 2008 Scientific Narre Conrrhon Name No % No % No % No % Lepisosterdae Leprsosteus osseus Longnose gar 3 0 08 % 3 008% 2 0 05 % Clupeidae Alosapseudoharengus Alewife 1 003% Dorosoma cepedianum Gizzard shad 3 1 17% 11 029% 1 003% 4 0 10 % Dorosomapetenense Threadfin shad 933 2482% 1,163 3045% 1,111 2741% Cypnmdae Ctenopharyngodon rdella Grass carp 3 0 08 % 2 005% Cypnnellanrvea Whdefnshner 6 233% 80 213% 85 223% 250 617% Cypnnus carpro Cannon carp 13 506% 29 077% 2 0 05 % 1 0 02 % Hybognathus regius Eastern silvery mnnow 1 0 03 % Notemrgonus crysoleucas Golden shiner 2 005% Notroprs hudsonrus Spottail shiner 24 064 Catostomrdae Carprodes cypnnus Qwllback 7 272% Moxostoma collapsum Notchlip redhorse 1 039% Ictalurrdae Amerurus catus Wide catfish 2 078% 11 0 29 % 1 0 03 % Amerurus platycephalus Flat bullhead 1 039% 2 005% Ictalurus furcatus Blue catfish 1 003% Ictalurus punctatus Channel catfish 1 0 39 % 7 019% 1 002% Pyfodichs olrvans Flathead catfish 1 003% 1 0 03 % 1 0 02 % Moronidae Moron saxabhs Striped bass 1 002% Centrarchrdae Lepomrsauntus Redbreast sunfish 87 3385% 1,776 4725% 1,672 4377% 1,213 2992% Lepomrs gibbosus FLrrpknseed 6 016% Lepomrs gulosus Warmouth 1 039% 13 035% 16 042% 18 044% Lepomrs hybrid Hybrid sunfish 2 0 78 % 3 008% 5 013% 12 0 30 % Lepomrs macrochrrus Bluegdl 45 17 51 % 408 1085% 486 12 72 % 1,048 2585% Lepomrs mrcrolophus Redear sunfish 25 973% 27 072% 94 246% 116 2 86 % Micropterus punctulatus Spotted bass 3 007% Micropterus salmoides Largemouth bass 58 2257% 383 1019% 254 665% 262 646% Micropterus hybrid Hybrid black bass 4 010% Pomows annularis Wide crappie 3 1 17% Pomoxrs nrgromaculatus Black crappie 2 0 78 % 22 059% 1 0 03 % 2 0 05 % Percrdae Etheostoma olmstedi Tessellated darter 4 010% 3 007% Perca flavescens Yellow perch 17 045% 27 0 71 % Total No. Individuals 257 100.00% 3,759 100 00 % 3,820 100 00 % 4,064 100 00 % Total No. Species 1s 20 18 17 5-9 Table 5-3. Number of individuals, biomass, and number of species collected during spring electrofishing of five 300-m transects downlake and five 300-m transects uplake of the RSS discharge canal in Mountain Island Lake, 1993 — 1997, 1999 — 2008. Year _ 1993 1994 1995 1996 1997 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 Average Uplake Total No Indimduals 475 454 355 854 412 843 1,273 1,769 1,862 2,123 955 1,292 468 1,009 2,140 1,086 Biomass (kg) 13418 81 19 12039 95 58 9843 5736 4889 75 58 51 98 5833 64 93 5827 4568 78 22 131 23 80.02 Total No Species 15 16 10 13 11 14 14 14 13 15 15 14 12 13 19 14 Downlake Total No Indimduals 307 388 368 356 519 590 874 1,437 1,140 1,434 1,263 706 1,278 1,389 1,379 895 Biomass (kg) 58 36 8267 4757 7198 35 94 29 12 5623 51 30 3578 4990 38 42 81 61 5336 57 95 69 55 54.65 Total No Species 12 12 10 10 12 9 16 11 10 14 12 13 12 11 10 12 Table 5-4. Number of individuals and number of species collected during spring electrofishing of ten 300-m transects in the vicinity of the RSS discharge canal in Mountain Island Lake, 1994 —1997 and 1999, 2000 — 2003, and 2004 —2008. 1994 - 1997, 1999 2000-2003 2004-2008 Scientific Name Common Name No oda No % No oda Lepisosteidae Leptsosteus osseus Longnose gar 2 004% 3 003% 2 002% Clupeidae Alosa pseudoharengus Alew ite 8 007% Dorosoma cepedianum Gizzard shad 91 177% 32 027% 46 039% Dorosoma petenense Threadfin shad 1 002% 22 019% Cyprinidae Ctenopharyngodon Idella Grass carp 3 003% 9 008% Cypnnella chlorrstia Greenfin shiner 13 025% 23 019% 5 004% Cyprnnella nuvea Whitefin shiner 130 253% 354 297% 929 782% Cyprnnus carpuo Common carp 70 136% 17 014% 32 027% Nocomis leptocephalus Bluehead chub 1 0 01 % Notemigonus crysoleucas Golden shiner 2 004% 8 007% 3 003% Notropus hudsonius Spottad shiner 20 039% 23 019% 1 0 01 % Notropis procne Sw allow tad shiner 3 006% 3 003% 8 007% Catostom idae Carptodes cyprnnus Qwllback 1 002% 2 002% Moxostoma collapsum Notchlip redhorse 2 004% Ictaluridae Ameiurus catus White catfish 1 002% 1 001% lctalurus punctatus Channel catfish 14 027% 16 013% 25 021% Salmon idae Oncorhynchus mykiss Rainbow trout 1 0 01 % Poeciliidae Gambusia holbrooki Eastern mosquitofish 4 003% 4 003% Moronidae Morone americana White perch 1 0 01 % 14 012% Morone chrysops White bass 2 004% Centrarchidae Lepomis auntus Redbreast sunfish 2,269 4415% 3,602 3024% 3,272 2754% Lepomis glbbosus Pumpkinseed 7 014% 1 001% Lepomis gulosus Warmouth 42 082% 82 069% 53 045% Lepomis hybrid Ffybril sunfish 17 033% 22 018% 39 033% Lepomis macrochirus Bluegdl 741 1442% 5,678 4767% 5,193 4372% Lepomis mucrolophus Redear sunfish 145 282% 1,257 1055% 1,357 1142% Micropterus punctulatus Spotted bass 2 002% Micropterus salmoides Largemouth bass 1,435 2792% 725 609% 726 611% Pomoxis nugromaculatus Black crappie 3 006% 14 012% 22 0.19% Percidae Etheostoma fustforme Sw amp darter 1 002% 1 0 01 % Etheostoma olmstedi Tessellated darter 1 002% 2 002% 12 010% Perca flavescens Yellow perch 126 245% 39 033% 92 077% Total No. Individuals 5,139 100.00% 11,912 100.00% 11,879 100.00% Total No. Species 24 23 26 5-11 Table 5-5. Number, percentage, average length, average density, and forage fish population estimates in Mountain Island Lake, 1993 — 1997, 1999 — 2008, from mid-September purse seine and hydroacoustic surveys. Year (Purse seine collection date) 1994 1996 1996 1997 1999 2000 2001 2002 2003 2004 2006 2006 2007 2008 Species (9/21) (7120) (9/18) (10/16) (9/22) (9/19) (9/12) (9123) (9115) (9/13) (9/19) (9/19) (9/13) (9110) Threadfin shad Number 580 825 1,169 182 570 1,984 1,580 3,336 656 522 3,970 4,450 9,275 5,963 Percentage 9949 81 93 99 57 10000 9948 83 28 89 29 9010 1692 8021 95 84 8712 9976 9586 Average length (mm) 538 41 9 501 59 2 54 0 530 51 7 438 58 2 51 9 523 51 9 52 2 54 8 Gizzard shad Number 3 182 5 2 Percentage 0 51 18 07 043 027 Average length (mm) 773 72 4 76 4 840 Alewife Number 3 398 190 366 3,220 127 172 659 22 257 Percentage 052 1672 10 71 9 89 8309 1958 4 15 1289 024 414 Average length (mm) 720 748 61 6 65 6 671 678 65 7 639 560 61 3 Hydroacoustic Data Density (No1ha) 3,867 4,312 6,798 998 4,413 2,530 4,554 3,752 2,366 603 3,611 2,952 2,836 2,268 Population estimate 3,852,000 4,295,000 6,771,000 994,000 4,395,000 2,520,000 4,536,000 3,737,000 2,357,000 601,000 3,597,000 2,940,000 2,825,000 2,259,000 800 700 600 E 0 500 U) Z 400 0 z 300 200 100 0 1994 16 14 12 E 0 10 U) 8 U N CL fA 0 6 Z 4 2 0 1994 1996 1998 2000 2002 2004 2006 2008 1996 1998 2000 2002 2004 2006 2008 Figure 5-1. Number of fish (a), and fish species (b) collected from electrofishing a 100-m transect at each of three locations (B, F, and G) during winter electrofishing in Mountain Island Lake, 1994 — 2008. 5-13 800 700 600 E 0 500 s w 400 0 z 300 200 100 0 16 14 12 E 0 10 U 8 N CL (0 6 0 z 4 2 0 1994 1996 1998 2000 2002 2004 2006 2008 b X B -*- F -A G 1994 1996 1998 2000 2002 2004 2006 2008 Figure 5-2. Number of fish (a), and fish species (b) collected from electrofishing a 100-m transect at each of three locations (B, F, and G) during summer electrofishing in Mountain Island Lake, 1994 — 2008. 5-14 16 14 12 10 rn 3 08 a� U) 06 04 02 00 1994 a X A -I-- B1�i C --e- G 16 14 12 a' 1 0 rn m 08 U) 06 04 02 00 1994 ■ 1996 1998 2000 2002 2004 2006 2008 b -XA tB 6 C -G-G 1996 1998 2000 2002 2004 2006 2008 16 14 C 12 °1 1 0 rn a� 08 0.6 04 02 00 1994 1996 1998 2000 2002 2004 2006 2008 Figure 5-3. Selenium concentrations in muscle tissue from common carp (a), sunfish (b), and bass (c) from four locations (A, B, C, and G) in Mountain Island Lake, 1994 — 2008. The only complete common carp samples were Locations A (2004 — 2006) and C (2005). 5-15 05 a 04 03 02 ■ ■ 01 00 1994 1996 1998 2000 2002 2004 2006 2008 05 04 03 CO 02 01 00 1994 b X A tB AC -e-G 05 04 03 z 02 01 00 1996 1998 2000 2002 2004 2006 2008 1994 1996 1998 2000 2002 2004 2006 2008 Figure 5-4. Arsenic concentrations in muscle tissue from common carp (a), sunfish (b), and bass (c) from four locations (A, B, C, and G) in Mountain Island Lake, 1994 — 2008. The only complete common carp samples were Locations A (2004 — 2006) and C (2005). 5-16 06 05 rn 04 rn 03 02 01 00 1995 06 05 a, 04 rn 3 03 02 01 00 1995 1997 1999 2001 2003 2005 2007 b - A t B -A C ---- G 1997 1999 2001 2003 2005 2007 06 C 0.5 o, 04 3 03 rn 02 01 00 1995 1997 1999 2001 2003 2005 2007 Figure 5-5. Mercury concentrations in muscle tissue from common carp (a), sunfish (b), and bass (c) from four locations (A, B, C, and G) in Mountain Island Lake, 1995 — 2008. The only complete common carp samples were Locations A (2004 — 2006) and C (2005). 5-17 120 100 a, 80 rn 60 N 40 20 00 1997 120 100 o, 80 rn 60 N 40 2.0 00 1997 X 1999 2001 2003 2005 2007 X A t A C -e-G 1999 2001 2003 2005 2007 120 C 100 a, 80 rn z 60 N 40 20 00 1997 1999 2001 2003 2005 2007 Figure 5-6. Zinc concentrations in muscle tissue from common carp (a), sunfish (b), and bass (c) from four locations (A, B, C, and G) in Mountain Island Lake, 1997 — 2008. The only complete common carp samples were Locations A (2004 — 2006) and C (2005). 5-18 LITERATURE CITED American Public Health Association (APHA); American Water Works Association; Water Environment Federation. 1998. Standard methods for the examination of water and wastewater. 20th Edition. American Public Health Association, Washington DC. Bales, JD, KM Sarver, and MJ Giorgino. 2001. Mountain Island Lake, North Carolina: Analysis of ambient conditions and simulation of hydrodynamics, constituent transport, and water -quality characteristics, 1996-97. Water -Resources Investigations Report 01-4138. U.S. Geological Survey, Raleigh. NC. 85 p. Brandt, SB. 1996. Acoustic assessment of fish abundance and distribution. Pages 385-432 in BR Murphy and DW Willis, editors. Fisheries Techniques. American Fisheries Society, Bethesda, MD. Brinkhurst, RO. 1974. The Benthos of Lakes. The MacMillan Press, London. 190 pp. Buetow, D. 2003. Mecklenburg County Water Quality Program lake summary, SWIM Phase 1, Part 2, 2001-2002. Mecklenburg County Department of Environmental Protection (MCDEP). Charlotte, NC. Buetow, D. 2008. 2007 Lake Monitoring Report. Mecklenburg County Water Quality Program, SWIM Phase I Part 2 -CO. Final Report for FY2007-2008. 14 p. Coughlan, DJ. 1995. Contaminants in largemouth bass collected from the Catawba River (NC/SC). Duke Power Company Scientific Services Research Report 95-01. Huntersville, NC. Duke Power Company. 1993. Thermal limit evaluation report for Riverbend Steam Station. Duke Power Company, Charlotte, NC. Duke Power. 1994. Assessment of balanced and indigenous populations in Mt. Island Lake near Riverbend Steam Station. Duke Power Company, Charlotte, NC. Duke Power. 2001. Assessment of Balanced and Indigenous Populations in Mountain Island Lake Near Riverbend Steam Station. Duke Power, Charlotte, NC. Duke Power. 2004. Assessment of Balanced and Indigenous Populations in Mountain Island Lake Near Riverbend Steam Station. Duke Power, Charlotte, NC. Harden, CW and SM Reid. 1991. Trace elements in Mountain Island Lake water, sediments, and fish. Duke Power Company Research Report PES/91-05. Huntersville, NC. L-1 Hayes, DB, CP Ferrier, and WW Taylor. 1996. Active fish capture methods. Pages 193-220 in BR Murphy and DW Willis, editors. Fisheries Techniques. American Fisheries Society, Bethesda, MD. Moore, JW and S Ramamoorthy. 1984. Heavy metals in natural waters. Springer -Verlag, New York, NY. National Geographic Holdings, Inc. 2001. Seamless USGS Topographic Maps on CD-ROM: North Carolina. National Geographic Maps, San Francisco, CA. North Carolina Department of Health and Human Services (NCDHHS). 2007. NC fish advisory action levels for DDT, DDE, DDD, Dioxins, mercury, PCBs, PBDEs, and selenium. NCDHH NC Occupational and Environmental Epidemiology Branch. Raleigh, NC, April 2007. North Carolina Department of Environment and Natural Resources (NCDENR). 2004. Catawba River Basinwide Water Quality Plan: September 2004. NC Department of Environment and Natural Resources, Division of Water Quality — Planning. Raleigh, NC. NCDENR. 2005. Permit to discharge wastewater under the National Pollutant Discharge Elimination System: Duke Energy Corporation, Riverbend Steam Station, Gaston County, NC. NCDENR. 2006. Standard operating procedures. Fish tissue assessments. NCDENR, Division of Water Quality, Environmental Sciences Section. Raleigh, NC. NCDENR. 2007a. "Redbook" Surface waters and wetlands standards. NC Administrative Code 15A NCAC 02B .0100, .0200 & .0300, Amended effective May 1, 2007. NCDENR, Division of Water Quality, Raleigh, NC. NCDENR. 2007b. Final North Carolina Water Quality Assessment and Impaired Waters List (2006 Integrated 305(b) and 303(d) Report), approved May 17, 2007. NCDENR, Division of Water Quality. Raleigh, NC. NCDENR. 2009. Division of Water Resources: Drought Monitoring. Web site: http://www.ncwater.ora/Drought Monitoring/ (Accessed May 17, 2009). National Oceanic and Atmospheric Administration (NOAA). 2008a. 2007 Local climatological data: Annual summary with comparative data, Charlotte, North Carolina (KCLT). U.S. Department of Commerce, National Climatic Data Center, Asheville, NC. NOAA. 2008b. August 2008 local climatological data: Charlotte, NC. U.S. Department of Commerce, National Climatic Data Center, Asheville, NC. L-2 SAS Institute Inc. 2002 — 2004. SAS OnlineDocTM, Version Nine. SAS Institute Inc., Cary, NC. United States Environmental Protection Agency (USEPA). 1975. U.S. Environmental Protection Agency National Eutrophication Survey. Report on Mountain Island Lake, Gaston and Mecklenburg Counties, North Carolina, EPA Region IV, Working Paper No. 386. Pacific Northwest Environmental Research Laboratory, Las Vegas, NV. 15 pp. USEPA. 1976. Quality criteria for water. ("Red Book"). PB -263 943. USEPA, Washington, DC. USEPA. 1977. 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Research Report No. 119. Water Resources Research Institute, University of North Carolina. Raleigh, NC. 224 p. L-3 APPENDIX TABLES Appendix Table A-1. Species composition, number of individuals, catch per unit effort (No./100 m), number of species, temperature, and dissolved oxygen concentration at three electrofishing locations (B, F, and G) in Mountain Island Lake, January 22, 2004. Locations B F G Total Saentdic Name Common Name No No No No % Le pis oste idae Lepisosteus osseus Longnose gar 5 1 6 067% Clupeidae Dorosoma cepedianum Gizzard shad 2 2 022% Dorosoma petenense Threadfin shad 441 441 4895% Cyprinidae Cypnnella rnvea Whitefin shiner 32 32 355% Cypnnus carpio Common carp 1 1 0 11 % Notemigonus crysoleucas Golden shiner 1 1 2 022% Poeciliidae Gambusia holbrooki Eastern mosgwtofish 1 1 011% Moronidae Morone saxatilis Striped bass 2 2 022% Ce ntrarch idae Lepomis auntus Redbreast sunfish 58 8 27 93 1032% Lepomis gulosus Warmouth 1 2 3 033% Lepomis hybrid Hybrid sunfish 1 1 011% Lepomis macrochirus Bluegill 58 26 135 219 24 31 % Lepomis microlophus Redear sunfish 21 9 3 33 366% Micropterus salmoides Largemouth bass 16 38 2 56 622% Pomoxis mgromaculatus Black crappie 8 8 089% Pe rcidae Perca flavescens Yellow perch 1 1 0 11 % Total No. Individuals 156 574 171 901 10000% CPUE (No. fish/100 m) 780 2870 855 1502 Total No. Species 6 13 7 15 Water Temperature (' C) 100 181 100 Dissolved Oxygen (mg/L) 105 100 106 A-1 Appendix Table A-2. Species composition, number of individuals, catch per unit effort (No./100 m), number of species, temperature, and dissolved oxygen concentration at three electrofishing locations (B, F, and G) in Mountain Island Lake, January 12, 2005. Locations B F G Total Scientific Name Common Name No No No. No % Cyprinidae Cyprrnella mvea Whitefin shiner 46 46 1559% Notropis hudson►us Spottad shiner 3 3 102% Centrarchidae Lepomis auntus Redbreast sunfish 12 18 36 66 2237% Lepomis hybrid Hybrid sunfish 2 2 068% Lepomis macrochirus Bluegdl 18 42 33 93 3153% Lepomis microlophus Redear sunfish 33 8 3 44 1492% Micropterus salmoides Largemouth bass 9 21 11 41 1390% Total No. Individuals 121 91 83 295 10000% CPUE(No. fish/100 m) 605 455 415 492 Total No. Species 6 4 4 6 Water Temperature (° C) 118 127 122 Dissolved Oxygen (mg/L) 9 9 9 9 103 A-2 Appendix Table A-3. Species composition, number of individuals, catch per unit effort (No./100 m), number of species, temperature, and dissolved oxygen concentration at three electrofishing locations (B, F, and G) in Mountain Island Lake, January 12, 2006. Locations B F G Total Scientific Name Common Name No No No No % Leplsosteidae Lepisosteus osseus Longnose gar 1 1 006% Clupeidae Dorosoma ceped►anum Gizzard shad 2 2 012% Dorosoma petenense Threadfin shad 1,373 5 1,378 8402% Cyprinidae Cypnnella mvea Whdefin shiner 13 13 079% Cyprmus Carpio Common carp 1 1 006% Notemigonus crysoleucas Golden shiner 2 2 012% Centrarchidae Lepomis aurntus Redbreast sunfish 9 20 39 68 415% Lepomis gulosus Warmouth 1 2 3 018% Lepomis hybrid Hybrid sunfish 1 1 006% Lepomis macrochirus Bluegdl 10 47 72 129 787% Lepomis microlophus Redear sunfish 3 13 9 25 152% Micropterus punctulatus Spotted bass 1 1 006% Micropterus salmoides Largemouth bass 3 8 5 16 098% Total No. Individuals 1,402 93 145 1,640 10000% CPIE(No. fish/100 m) 701 465 725 2733 Total No. Species 8 7 7 12 Water Temperature (° C) 107 117 116 Dissolved Oxygen (mg/L) 101 99 100 A-3 Appendix Table A-4. Species composition, number of individuals, catch per unit effort (No./100 m), number of species, temperature, and dissolved oxygen concentration at three electrofishing locations (B, F, and G) in Mountain Island Lake, January 3, 2007. Locations B F G Total Scientific Name Common Name No No No No % Le pis oste idae Leptsosteus osseus Longnose gar 2 2 016% Clupeidae Dorosoma cepedianum Gizzard shad 1 2 3 024% Dorosoma petenense Threadfin shad 582 582 4652% Cyprinidae Cypnnella mvea Whitefin shiner 77 2 18 97 775% Moronidae Morone saxahlis Striped bass 1 1 008% Centrarchidae Lepomis auntus Redbreast sunfish 19 29 148 196 1567% Lepomis gulosus Warmouth 4 4 032% Lepomis hybrid Hybrid sunfish 1 1 2 016% Lepomis macrochirus Bluegill 17 77 167 261 2086% Lepomis microlophus Redear sunfish 2 5 41 48 384% Micropterus salmoides Largemouth bass 13 29 8 50 400% Micropterus hybrid Hybrid black bass 1 3 4 032% Pomoxis nigromaculatus Black crappie 1 1 008% Total No. Individuals 129 730 392 1,251 10000% CPUE(No. fish/100 m) 64 5 365 196 2085 Total No. Species 6 10 8 12 Wate r Te m pe ratu re (' C) 132 176 141 Dissolved Oxygen (mg/L) 8 9 8 9 8 9 A-4 Appendix Table A-5. Species composition, number of individuals, catch per unit effort (No./100 m), number of species, temperature, and dissolved oxygen concentration at three • electrofishing locations (B, F, and G) in Mountain Island Lake, January 3, 2008. Locations B F G Total Scientific Name Cominon Name No No. No No % Clupeidae Dorosoma cepedianum Gizzard shad 1 1 010% Dorosoma petenense Threadfin shad 1 1 010% Cyprinidae Ctenopharyngodon Idella Grass carp 1 1 010% Cyprnnella mvea Whitefin shiner 95 2 97 979% Cypnnus carpio Common carp 1 1 010% Notemigonus crysoleucas Golden shiner 1 1 010% Notropis hudsomus Spottad shiner 4 4 040% Notropis procne Swallow tad shiner 49 49 494% Ictaluridae lctalurus punctatus Channel catfish 3 3 030% Poeciliidae Gambusia holbrooki Eastern mosgwtofish 1 1 2 020% Moronidae Morone saxatihs Striped bass 7 7 0 71 % Centrarchidae Lepomis auntus Redbreast sunfish 149 109 83 341 34 41 % Lepomis g►bbosus Pumpkinseed 4 4 040% Lepomis hybrid Hybrid sunfish 3 3 030% Lepomis macrochirus Bluegdl 177 85 172 434 4379% Lepomis microlophus Redear sunfish 7 3 4 14 141% Micropterus punctulatus Spotted bass 2 2 020% Micropterus salmoides Largemouth bass 7 7 8 22 222% Micropterus hybrid Hybrid black bass 1 1 010% Pomoxis mgromaculatus Black crappie 2 2 020% Percidae Perca flavescens Yellow perch 1 1 010% Total No. Individuals 490 226 275 991 10000% CPUE(No. fish/100 m) 245 113 1375 1652 Total No. Species 9 12 8 19 Water Temperature (" C) 76 176 106 Dissolved Oxygen (mg/L) 11 0 107 107 A-5 Appendix Table A-6. Species composition, number of individuals, catch per unit effort (No./100 m), number of species, temperature, and dissolved oxygen concentration at three electrofishing locations (B, F, and G) in Mountain Island Lake, July 8, 2004. Total No. Individuals 252 Locations 318 663 10000% CPIJE (No. fish/100 m) 1260 465 1590 1105 B F G Total Scientific Name Conmon Name No No No No % Cyprinidae Ctenopharyngodon idella Grass carp 1 1 015% Cypnnella mvea Whitefin shiner 23 11 1 35 528% Ce ntrarchidae Lepomis aurntus Redbreast sunfish 123 15 188 326 4917% Lepomis gulosus Warmouth 2 6 8 1 21 % Lepomis hybrid Hybrid sunfish 2 1 3 045% Lepomis macrochirus Bluegdl 61 36 82 179 2700% Lepomis microlophus Redear sunfish 23 1 10 34 513% Micropterus punctulatus Spotted bass 1 1 015% Micropterus salmoides Largemouth bass 17 28 29 74 1116% Percidae Etheostoma olmstedi Tessellated darter 1 1 2 030% Total No. Individuals 252 93 318 663 10000% CPIJE (No. fish/100 m) 1260 465 1590 1105 Total No. Species 7 7 7 9 Water Temperature (° C) 272 330 316 Dissolved Oxygen (mg/L) 55 64 72 A-6 Appendix Table A-7. Species composition, number of individuals, catch per unit effort (No./100 m), number of species, temperature, and dissolved oxygen concentration at three electrofishing locations (B, F, and G) in Mountain Island Lake, July 5, 2005. Scientific Name Common Name Locations B F No No G No Total No % Clupeidae 405 1155 822 Total No. Species 7 5 7 10 Dorosoma cepedianum Gizzard shad 346 301 1 1 020% Cyprinidae Ctenopharyngodon Idella Grass carp 1 1 020% Cypnnella nivea Whitefm shiner 27 6 3 36 730% Ictaluridae Pylodictis olivaris Flathead catfish 1 1 020% Centrarchidae Lepomis auntus Redbreast sunfish 95 21 117 233 4726% Lepomis gulosus Warmouth 2 2 0 41 % Lepomis hybrid Hybrid sunfish 2 2 041% Lepomis macrochirus Bluegdl 37 24 95 156 3164% Lepomis microlophus Redear sunfish 3 7 10 203% Micropterus salmoides Largemouth bass 17 29 4 50 1014% Pomoxis nigromaculatus Black crappie 1 1 020% Total No. Individuals 181 81 231 493 10000% CPUE(No. fish/100 m) 905 405 1155 822 Total No. Species 7 5 7 10 Water Temperature (° C) 272 346 301 Dissolved Oxygen (mg/L) 61 62 71 A-7 Appendix Table A-8. Species composition, number of individuals, catch per unit effort (No./100 m), number of species, temperature, and dissolved oxygen concentration at three electrofishing locations (B, F, and G) in Mountain Island Lake, July 25, 2006. Locations B F G Total Scientific Name Common Name No No No No % Clupeldae Dorosoma petenense Threadfm shad 525 4 529 7007% Cyprinidae Cypnnella mvea Whitefin shiner 1 14 1 16 212% Centrarchidae Lepomis auntus Redbreast sunfish 50 5 31 86 1139% Lepomis gulosus Warmouth 1 1 013% Lepomis hybrid Hybrid sunfish 1 1 013% Lepomis macrochirus Bluegdl 46 24 14 84 11 13% Lepomis microlophus Redear sunfish 6 5 11 146% Micropterus salmoides Largemouth bass 16 8 3 27 358% Total No. Individuals 120 576 59 755 10000% CPUE (No. fish/100 m) 600 2880 29 5 1258 Total No. Species 5 5 7 7 Water Temperature (` C) 302 352 322 Dissolved Oxygen (mg/L) 6 5 6 8 7 3 A-8 Appendix Table A-9. Species composition, number of individuals, catch per unit effort (No./100 m), number of species, temperature, and dissolved oxygen concentration at three electrofishing locations (B, F, and G) in Mountain Island Lake, July 11, 2007. Locations B F G Total Scientific Name Common Name No No No No % Clupeidae Dorosoma cepedianum Gizzard shad 2 2 0 31 % Cyprinidae Ctenopharyngodon Idella Grass carp 2 2 0 31 % Cypnnella nivea Whitefin shiner 15 34 3 52 813% Centrarchidae Lepomis auntus Redbreast sunfish 71 18 242 331 5172% Lepomts gibbosus Pumpkinseed Lepomis gulosus Warmouth 10 10 156% Lepomis hybrid Hybrid sunfish 1 1 016% Lepomis macrochirus Bluegdl 44 32 119 195 3047% Lepomis microlophus Redear sunfish 7 4 17 28 438% Micropterus punctulatus Spotted bass 1 1 016% Micropterus salmoides Largemouth bass 3 8 7 18 2 81 % Total No. Individuals 142 98 400 640 10000% CPUE(No. fish/100 m) 71 0 49 0 2000 1067 Total No. Species 6 6 7 9 Water Temperature (° C) 29 0 337 309 Dissolved Oxygen (mg/L) 6 5 6 4 7 3 A-9 Appendix Table A-10. Species composition, number of individuals, catch per unit effort (No./100 m), number of species, temperature, and dissolved oxygen concentration at three electrofishing locations (B, F, and G) in Mountain Island Lake, July 29, 2008. Locations B F G Total Scientific Name Conrnon Name No No No No % Cyprinidae Cyprnnella mvea Whitefin shiner 4 58 4 66 740% Cypnnus carpio Common carp 1 1 011% Ictaluridae Ictalurus punctatus Channel catfish 1 1 011% Centrarchidae Lepomis auntus Redbreast sunfish 89 28 255 372 4170% Lepomis gulosus Warmouth 3 3 034% Lepomis hybrid Hybrid sunfish 2 2 4 045% Lepomis macrochirus Bluegdl 56 141 171 368 4126% Lepomts microlophus Redear sunfish 5 8 13 146% Micropterus punctulatus Spotted bass 2 2 022% Micropterus salmoides Largemouth bass 39 4 18 61 684% Percidae Etheostoma olmstedi Tessellated darter 1 1 011% Total No. Individuals 195 234 463 892 10000% CPUE(No. fish/100 m) 975 117 2315 1487 Total No. Species 7 5 7 10 Water Temperature (" C) 301 357 327 Dissolved Oxygen (mg/L) 78 76 76 A-10