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HomeMy WebLinkAboutNC0020800_Renewal (Application)_20170321Water Resources ENVIRONMENTAL QUALITY March 21, 2017 Ms. Nancy Curtis, Mayor Town of Andrews PO Box 1210 Andrews, NC 28901 Subject: Renewal Application Application No. NCO020800 Andrews WWTP Cherokee County Dear Ms. Curtis: ROY COOPER Governor MICHAEL S. REGAN Secretary S. JAY ZIMMERMAN Director . The Water Quality Permitting Section acknowledges receipt of your permit application and supporting documentation received on March 20, 2017. The primary reviewer for this renewal application is Julie Grzyb. The primary reviewer will review your application, and she will contact you if additional information is required to complete your permit renewal. Per G.S. 150B-3 your current permit does not expire until permit decision on the application is made. Continuation of the current permit is contingent on timely and sufficient application for renewal of the current permit. Please respond in a timely manner to requests for additional information necessary to complete the permit application. If you have any additional questions concerning renewal of the subject permit, please contact Julie Grzyb at 919-807-6389 or Julie.Grzyb@ncdenr.gov. cc: Central Files NPDES- Asheville Regional Office Sincerely, ? tiea %&*vd Wren Thedford Wastewater Branch State of North Carolina I Environmental Quality I Water Resources 1617 Mail Service Center I Raleigh, North Carolina 27699-1617 919-807-6300 3/15/17 NPDES PERMIT RENEWAL APPLICATION FOR ANDREWS, NC WASTEWATER TREATMENT PLANT Permit #: NCO020800 Facility Address: 464 Reagan Ave. Andrews, NC. 28901 Included: • NPDES Permit Renewal Application Attached Documentation: RECEIVEDINCDEQUYR MAR 2 0 2017 P �terQuality ttiOg Section • Pollutant Scan Analysis Data for Part B, Section 13.6 (3 sample analysis from 2/13, 5/14, and 2/15) • Pollutant Scan Analysis Data for Part D (3 sample analysis from 2/13, 5/14, and 2/15) • Complete Toxicity Test Results for 2016 for Part E, Section E • Topographic Map • Basic Process Flow Diagram FlACILITY'NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE FORM ' 2A N"P'DES FORM 2A �4PPLICATION ®`UER\/LEVII ' �" NPDES APPLICATION OVERVIEW Form 2A has been developed in a modular format and consists of a "Basic Application Information" packet and a "Supplemental Application Information" packet. The Basic Application Information packet is divided into two parts. All applicants must complete Parts A and C. Applicants with a design flow greater than or equal to 0.1 mgd must also complete Part B. Some applicants must also complete the Supplemental Application Information packet. The following items explain which parts of Form 2A you must complete. BASIC APPLICATION INFORMATION: A. Basic Application Information for all Applicants. All applicants must complete questions A.1 through A.8. A treatment works that discharges effluent to surface waters of the United States must also answer questions A.9 through A.12. B. Additional Application Information for Applicants with a Design Flow >_ 0.1 mgd. All treatment works that have design flows greater than or equal to 0.1 million gallons per day must complete questions B.1 through 13.6. C. Certification. All applicants must complete Part C (Certification). SUPPLEMENTAL APPLICATION INFORMATION: D. Expanded Effluent Testing Data. A treatment works that discharges effluent to surface waters of the United States and meets one or more of the following criteria must complete Part D (Expanded Effluent Testing Data): 1. Has a design flow rate greater than or equal to 1 mgd, 2. Is required to have a pretreatment program (or has one in place), or 3. Is otherwise required by the permitting authority to provide the information. E. Toxicity Testing Data. A treatment works that meets one or more of the following criteria must complete Part E (Toxicity Testing Data): 1. Has a design flow rate greater than or equal to 1 mgd, 2. Is required to have a pretreatment program (or has one in place), or 3. Is otherwise required by the permitting authority to submit results of toxicity testing. F. Industrial User Discharges and RCRA/CERCLA Wastes. A treatment works that accepts process wastewater from any significant industrial users (SIUs) or receives RCRA or CERCLA wastes must complete Part F (Industrial User Discharges and RCRA/CERCLA Wastes). SIUs are defined as: 1. All industrial users subject to Categorical Pretreatment Standards under 40 Code of Federal Regulations (CFR) 403.6 and 40 CFR Chapter I, Subchapter N (see instructions); and 2. Any other industrial user that: a. Discharges an average of 25,000 gallons per day or more of process wastewater to the treatment works (with certain exclusions); or b. Contributes a process wastestream that makes up 5 percent or more of the average dry weather hydraulic or organic capacity of the treatment plant; or C. Is designated as an SIU by the control authority. G. Combined Sewer Systems. A treatment works that has a combined sewer system must complete Part G (Combined Sewer Systems). Y ALL APPLICANTS`'MUST::COMPLETE;"PART'-C (CERTIFICATION) EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Pagel of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE BASIC,APPLICATION INFORMATION PART A. BASIC APPLICATION INFORMATION FORALL APPLICANTS: All treatment works must complete questions A.1 through A.8 of this Basic Application Information Packet. A.I. Facility Information. Facility Name ANDREWS WASTEWATER TREATMENT PLANT Mailing Address PO BOX 1210 ANDREWS, NC 28901 Contact Person NANCY CURTIS TIM WOOD Title MAYOR WWTP ORC Telephone Number (828) 321-3113 (828) 321-3006 Facility Address 464 REAGAN AVE. (not P.O. Box) ANDREWS NC 28901 A.2. Applicant Information. If the applicant is different from the above, provide the following: Applicant Name TOWN OF ANDREWS Mailing Address PO BOX 1210 ANDREWS NC 28901 Contact Person NANCY CURTIS Title MAYOR Telephone Number (828) 321-3113 Is the applicant the owner or operator (or both) of the treatment works? ® owner ❑ operator Indicate whether correspondence regarding this permit should be directed to the facility or the applicant. ❑ facility ® applicant A.3. Existing Environmental Permits. Provide the permit number of any existing environmental permits that have been issued to the treatment works (include state -issued permits). NPDES NCO020800 PSD UIC Other RCRA Other A.4. Collection System Information. Provide information on municipalities and areas served by the facility. Provide the name and population of each entity and, if known, provide information on the type of collection system (combined vs. separate) and its ownership (municipal, private, etc.). Name Population Served Type of Collection System Ownership Town of Andrews 1500 Separate Municipal Total population served EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 2 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE A.S. Indian Country. a. Is the treatment works located in Indian Country? ❑ Yes ® No b. Does the treatment works discharge to a receiving water that is either in Indian Country or that is upstream from (and eventually flows through) Indian Country? ❑ Yes ® No A.S. Flow. Indicate the design flow rate of the treatment plant (i.e., the wastewater flow rate that the plant was built to handle). Also provide the average daily flow rate and maximum daily flow rate for each of the last three years. Each year's data must be based on a 12 -month time period with the 12th month of "this year' occurring no more than three months prior to this application submittal. a. Design flow rate 1.5 mgd Two Years Ago Last Year This Year b. Annual average daily flow rate 0.616 (2014) 0.713 (2015) 0.570 (2016) C. Maximum daily flow rate 1.850 (10/15/14) 2.143 (12/31/15) 1.961 (2/25/16) A.7. Collection System. Indicate the type(s) of collection system(s) used by the treatment plant. Check all that apply. Also estimate the percent contribution (by miles) of each. ® Separate sanitary sewer 100 % ❑ Combined storm and sanitary sewer % A.8. Discharges and Other Disposal Methods. a. Does the treatment works discharge effluent to waters of the U.S.? ® Yes ❑ No If yes, list how many of each of the following types of discharge points the treatment works uses: I. Discharges of treated effluent 1 ii. Discharges of untreated or partially treated effluent None iii. Combined sewer overflow points N/A iv. Constructed emergency overflows (prior to the headworks) NIA V. Other I NIA b. Does the treatment works discharge effluent to basins, ponds, or other surface impoundments that do not have outlets for discharge to waters of the U.S.? ❑ Yes ® No If yes, provide the following for each surface impoundment: Location: k Annual average daily volume discharge to surface impoundment(s) Is discharge ❑ continuous or ❑ intermittent? C. Does the treatment works land -apply treated wastewater? If yes, provide the following for each land application site: Location: Number of acres: Annual average daily volume applied to site: Is land application ❑ continuous or ❑ intermittent? d. Does the treatment works discharge or transport treated or untreated wastewater to another treatment works? 1. EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. r mgd ❑ Yes ® No mgd ❑ Yes ® No Page 3 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE If yes, describe the mean(s) by which the wastewater from the treatment works is discharged or transported to the other treatment works (e.g., tank truck, pipe). If transport is by a party other than the applicant, provide: Transporter Name Mailing Address Contact Person Title Telephone Number ( 1 For each treatment works that receives this discharge, provide the following: Name Mailing Address Contact Person Title Telephone Number ( 1 If known, provide the NPDES permit number of the treatment works that receives this discharge Provide the average daily flow rate from the treatment works into the receiving facility. mgd e. Does the treatment works discharge or dispose of its wastewater in a manner not included in A.8. through A.8.d above (e.g., underground percolation, well injection): ❑ Yes ® No If yes, provide the following for each disposal method: Description of method (including location and size of site(s) if applicable): Annual daily volume disposed by this method: Is disposal through this method ❑ continuous or ❑ intermittent? EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 4 of 22 n FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE WASTEWATER DISCHARGES: If you answered "Yes" to question A.8.a, complete questions A.9 through A.12 once for each outfall (including bypass points) through which effluent is discharged. Do not include information on combined sewer overflows in this section. If you answered "No" to question A.8.a, go to Part B, "Additional Application Information for Applicants with a Design Flow Greater than or Equal to 0.1 mgd." A.9. Description of Outfall. a. Outfall number 001 b. Location VALLEY RIVER, ANDREWS, NC. 28901 (City or town, if applicable) (Zip Code) (County) 35°11'51' (Latitude) C. Distance from shore (if applicable) d. Depth below surface (if applicable) e. Average daily flow rate f. Does this outfall have either an intermittent or a periodic discharge? If yes, provide the following information: Number f times per year discharge occurs: Average duration of each discharge: Average flow per discharge: Months in which discharge occurs: g. Is outfall equipped with a diffuser? (State) (Longitude) N/A ft. N/A ft. 0.633 mgd ❑ Yes ® No (go to A.9.g.) ❑ Yes ® No mgd A.10. Description of Receiving Waters. a. Name of receiving water VALLEY RIVER b. Name of watershed (if known) HIWASSEE RIVER WATERSHED United States Soil Conservation Service 14 -digit watershed code (if known): C. Name of State Management/River Basin (if known): United States Geological Survey 8 -digit hydrologic cataloging unit code (if known): d. Critical low flow of receiving stream (if applicable) acute cfs chronic cfs e. Total hardness of receiving stream at critical low flow (if applicable): mg/I of CaCO3 EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 5 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE A.11. Description of Treatment a. What level of treatment are provided? Check all that apply. E Primary ® Secondary ❑ Advanced ❑ Other. Describe: b. Indicate the following removal rates (as applicable): Design BOD5 removal or Design CBOD5 removal 85 % Design SS removal 85 % Design P removal % Design N removal % Other % C. What type of disinfection is used for the effluent from this outfall? If disinfection varies by season, please describe: CHLORINATION If disinfection is by chlorination is dechlorination used for this outfall? E Yes, ❑ No Does the treatment plant have post aeration? ❑ Yes E No A.12. Effluent Testing Information. All Applicants that discharge to waters of the US must provide effluent testing data for the following parameters. Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a minimum, effluent testing data must be based on at least three samples and must be no more than four and one-half years apart. Outfall number: 001 MAXIMUM DAILY VALUE - AVERAGE DAILY VALUE PARAMETER Value Units Value Units Number of Samples pH (Minimum) 5.8 S.U. pH (Maximum) 7.4 s.u. Flow Rate 2.685 MGD 0.684 MGD 1460 Temperature (Winter) 16.1 °C 11.35 °C 92 Temperature (Summer) 25.1 °C 21.5 °C 92 * For pH please report a minimum and a maximum daily value MAXIMUMDAILY'`` AVERAGE DAILY''DISCHARGE DISCHARGE ANALYTICAL POLLUTANT, METHOD ML/MDL Conc. Units Conc. Units Number of Samples CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS BIOCHEMICAL OXYGEN BOD5 74.8 MG/L 23.3 MG/L 1460 SM5210B 2.0 DEMAND (Report one) CBOD5 FECAL COLIFORM >9000 #/100ML 62 #/100ML 1460 SM9222D 1/100m1 TOTAL SUSPENDED SOLIDS (TSS) 95.6 MG/L 1 19.7 MG/L 1460 SM2540D 5.0 END OF' PART A. REFER TO, THE APPLICATION! OVERVIEW_(PAGE'I) TO DETERMINE'WHICH OTHER PARTS OF FORM.2A YOU ,MUST COMPLETE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 6 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE BASIC APPLICATION INFORMATION. - PART:B.- -ADDITIONAL APPLICATION INFORMATION FOR APPLICANTlq,WITH°'A DESIGN FLOW GREATER THAN OR. EQUAL- TO;0.1 MOD (100;000 gallons;per day). ^, All applicants with a design flow rate 2 0.1 mgd must answer questions B.1 through B.6. All others go to Part C (Certification). B.1. Inflow and Infiltration. Estimate the average number of gallons per day that flow into the treatment works from inflow and/or infiltration. 100,000 gpd Briefly explain any steps underway or planned to minimize inflow and infiltration. Ongoing identification and repair of problem areas by Sewer Collection Dept. B.2. Topographic Map. Attach to this application a topographic map of the area extending at least one mile beyond facility property boundaries. This map must show the outline of the facility and the following information. (You may submit more than one map if one map does not show the entire area.) a. The area surrounding the treatment plant, including all unit processes. b. The major pipes or other structures through which wastewater enters the treatment works and the pipes or other structures through which treated wastewater is discharged from the treatment plant. Include outfalls from bypass piping, if applicable. c. Each well where wastewater from the treatment plant is injected underground. d. Wells, springs, other surface water bodies, and drinking water wells that are: 1) within ''% mile of the property boundaries of the treatment works, and 2) listed in public record or otherwise known to the applicant. e. Any areas where the sewage sludge produced by the treatment works is stored, treated, or disposed. f. If the treatment works receives waste that is classified as hazardous under the Resource Conservation and Recovery Act (RCRA) by truck, rail, or special pipe, show on the map where the hazardous waste enters the treatment works and where it is treated, stored, and/or disposed. B.3. Process Flow Diagram or Schematic. Provide a diagram showing the processes of the treatment plant, including all bypass piping and all backup power sources or redunancy in the system. Also provide a water balance showing all treatment units, including disinfection (e.g., chlorination and dechlorination). The water balance must show daily average flow rates at influent and discharge points and approximate daily flow rates between treatment units. Include a brief narrative description of the diagram. B.4. Operation/Maintenance Performed by Contractor(s). Are any operational or maintenance aspects (related to wastewater treatment and effluent quality) of the treatment works the responsibility of a contractor? ® Yes ❑ No If yes, list the name, address, telephone number, and status of each contractor and describe the contractor's responsibilities (attach additional pages if necessary). Name: Earth Environmental Services Mailing Address: 75 Bison Ln. Murphy, NC. 28906 Telephone Number: (828) 837-9543 Responsibilities of Contractor: Laboratory Analysis/Sampling B.S. Scheduled improvements and Schedules of Implementation. Provide information on any uncompleted implementation schedule or uncompleted plans for improvements that will affect the wastewater treatment, effluent quality, or design capacity of the treatment works. If the treatment works has several different implementation schedules or is planning several improvements, submit separate responses to question B.5 for each. (If none, go to question B.6.) a. List the outfall number (assigned in question A.9) for each outfall that is covered by this implementation schedule. 001 b. Indicate whether the planned improvements or implementation schedule are required by local, State, or Federal agencies. ❑ Yes ® No EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 7 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE C. If the answer to B.5.b is "Yes," briefly describe, including new maximum daily inflow rate (if applicable). N/A d. Provide dates imposed by any compliance schedule or any actual dates of completion for the implementation steps listed below, as applicable. For improvements planned independently of local, State, or Federal agencies, indicate planned or actual completion dates, as applicable. Indicate dates as accurately as possible. Schedule Actual Completion Implementation Stage MM/DD/YYYY MM/DD/YYYY - Begin Construction 04/30/2017 (Tentative Date) - End Construction 12/31/2017 (Tentative Date) - Begin Discharge - Attain Operational Level e. Have appropriate permits/clearances concerning other Federal/State requirements been obtained? ® Yes ❑ No Describe briefly: B.6. EFFLUENT TESTING DATA (GREATER THAN 0.1 MGD ONLY). Applicants that discharge to waters of the US must provide effluent testing data for the following parameters. Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include information on combine sewer overflows in this section. All information reported must be based on data collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a minimum effluent testing data must be based on at least three pollutant scans and must be no more than four and on -half years old. Outfall Number: 001 MAXIMUM DAILY AVERAGE DAILY DISCHARGE DISCHARGE ANALYTICAL POLLUTANT METHOD MLJMDL Conc., Units. Conc. Units Number of Samples CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS AMMONIA (as N) CHLORINE (TOTAL RESIDUAL, TRC) DISSOLVED OXYGEN TOTAL KJELDAHL NITROGEN (TKN) NITRATE PLUS NITRITE NITROGEN OIL and GREASE PHOSPHORUS (Total) TOTAL DISSOLVED SOLIDS (TDS) OTHER ENO -OF PART B. REFER'TO THE AP.PLICATION,OVERVIEW:(PAGE 1).TO'DETERMINE WLHICH OTHER;PARTS .OF'FORM 2A YOU VUST COMPLETE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 8 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE 'BASIC' APP:LICAT1.0 INFORMATION r PART•C. C-ERTIFICATION!,:' All applicants must complete the Certification Section. Refer to instructions to determine who is an officer for the purposes of this certification. All applicants must complete all applicable sections of Form 2A, as explained in the Application Overview. Indicate below which parts of Form 2A you have completed and are submitting. By signing this certification statement, applicants confirm that they have reviewed Form 2A and have completed all sections that apply to the facility for which this application is submitted. Indicate which parts of Form 2A you have completed and are submitting: ® Basic Application Information packet Supplemental Application Information packet: ® Part D (Expanded Effluent Testing Data) ® Part E (Toxicity Testing: Biomonitoring Data) ® Part F (Industrial User Discharges and RCRA/CERCLA Wastes) ❑ Part G (Combined Sewer Systems) •,. ALL AP.;PLICANTS MUST-COMPLETE„TWE•FOL'LOWING CERTIF.ICATION., I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system or those persons directly responsible for gathering the information, the information is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Name and official title Ma or Nancy Curtis Signature Telephone number (828) 321-3113 Date signed Upon request of the permitting authority, you must submit any other information necessary to assure wastewater treatment practices at the treatment works or identify appropriate permitting requirements. SEND COMPLETED FORMS TO: NCDENR/ DWR Attn: NPDES Unit 1617 Mail Service Center Raleigh, North Carolina 27699-1617 EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 9 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NCO020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE SUPPLEMENTAL APPLICATION, INFORMATION PART D. fXPANDED: EFFLUENT TESTING DATA Refer to the directions on the cover page to determine whether this section applies to the treatment works. Effluent Testing: 1.0 mgd and Pretreatment Works. If the treatment works has a design flow greater than or equal to 1.0 mgd or it has (or is required to have) a pretreatment program, or is otherwise required by the permitting authority to provide the data, then provide effluent testing data for the following pollutants. Provide the indicated effluent testing information and any other information required by the permitting authority for each outfall through which effluent is discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected through analyses conducted using 40 CFR Part 136 methods. In addition, these data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. Indicate in the blank rows provided below any data you may have on pollutants not specifically listed in this form. At a minimum, effluent testing data must be based on at least three pollutant scans and must be no more than four and one-half years old. Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.) POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE _V ANALYTICAL METHOD MUMDL Conc. Units Mass Units Conc. Units Mass Units Number of Samples METALS (TOTAL RECOVERABLE), CYANIDE, PHENOLS, AND HARDNESS. ANTIMONY ARSENIC 0.010 Mg/I 0.010 Mg/I 3 BERYLLIUM CADMIUM 0.001 Mg/I 0.001 Mg/I 3 CHROMIUM 0.005 Mg/l 0.005 Mg/I 3 COPPER 0.067 Mg/I 0.033 Mg/I 3 LEAD 0.005 Mg/I 0.005 Mg/l 3 MERCURY 0.0002 Mg/l 0.0002 Mg/I 3 NICKEL 0.010 Mg/I 0.010 Mg/I 3 SELENIUM 0.010 Mg/I 0.010 Mg/I 3 SILVER 0.005 Mg/I 0.005 Mg/I 3 THALLIUM ZINC 0.080 Mg/I 0.044 Mg/I 3 CYANIDE 0.042 Mg/I 0.010 Mg/I 3 TOTAL PHENOLIC COMPOUNDS HARDNESS (as CaCO3) _LLUse this space (or a separate sheet) to provide information on other metals requested by the permit writer 4 EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 10 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NCO020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.) POLLUTANT MAXIMUM DAILY'yDISCHARGE AVERAGE•DAILY DISCHARGE= ` ANALYTICAL M ETHOD ' MLIMDL Cont s.. Units . Mass ` .,,Units ' Coni ,, Unrts Mass . .Units Number ..of _Samples VOLATILE ORGANIC COMPOUNDS ACROLEIN ACRYLONITRILE BENZENE BROMOFORM CARBON TETRACHLORIDE CHLOROBENZENE CHLORODIBROMO- METHANE CHLOROETHANE 2-CHLOROETHYLVINYL ETHER CHLOROFORM DICHLOROBROMO- METHANE 1,1-DICHLOROETHANE 1,2-DICHLOROETHANE TRANS-1,2-DICHLORO- ETHYLENE 1,1-DICHLORO- ETHYLENE 1,2-DICHLOROPROPANE 1,3-DICHLORO- PROPYLENE ETHYLBENZENE METHYL BROMIDE METHYL CHLORIDE METHYLENE CHLORIDE 1,1,2,2-TETRA- CHLOROETHANE TETRACHLORO- ETHYLENE TOLUENE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 11 of 22 FACILITY NAME AND PERMIT NUMBER: I ANDREWS WWTP, NCO020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.) _s POLLUTANT- '= MAXIMUM DAILY.DISCHARGE � :" AVERAGE DAILY -DISCHARGE' ANALYTICAL METHOD" �ryL7MDL_ Cont Units Mass 'Units ',:Cont ,`; Unit§ Mass_ � Units "' Number �, of �� Samples TRICHLOROETHANE TRICHLOROETHANE TRICHLOROETHANE TRICHLOROETHYLENE VINYL CHLORIDE Use this space (or a separate sheet) to provide information on other volatile organic compounds requested by the permit writer ACID -EXTRACTABLE COMPOUNDS P -CHLORO -M -CRESOL 2 -CHLOROPHENOL 2,4-DICHLOROPHENOL 2,4 -DIMETHYLPHENOL 4, 6 -DIN ITR O -O -C R ES O L 2,4-DINITROPHENOL 2-NITROPHENOL 4-NITROPHENOL PENTACHLOROPHENOL PHENOL 2,4,6 - TRICHLOROPHENOL Use this space (or a separate sheet) to provide information on other acid -extractable compounds requested by the permit writer BASE -NEUTRAL COMPOUNDS ACENAPHTHENE ACENAPHTHYLENE ANTHRACENE BENZIDINE BENZO(A)ANTHRACENE BENZO(A)PYRENE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 12 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NCO020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.) POLLUTANT " ;e. MAXIMUMrDAILY;DISCHARGE "" ::AVERAGE" DAILY"`DISCHARGE ANALYTICAL METHOD T MGMD'L Cont. _ Units " Mass �. ,. "-Units= Conc; Units - Mass` � Units .. Number ' of Samples 3,4 BENZO- FLUORANTHENE BENZO(GHI)PERYLENE BENZO(K) FLUORANTHENE BIS (2-CHLOROETHOXY) METHANE BIS (2-CHLOROETHYL)- ETHER BIS (2-CHLOROISO- PROPYL)ETHER BIS (2-ETHYLHEXYL) PHTHALATE 4-BROMOPHENYL PHENYLETHER BUTYL BENZYL PHTHALATE 2 -CHLORO - NAPHTHALENE 4-CHLORPHENYL PHENYLETHER CHRYSENE DI -N -BUTYL PHTHALATE DI-N-OCTYL PHTHALATE DIBENZO(A,H) ANTHRACENE 1,2 -DICHLOROBENZENE 1,3 -DICHLOROBENZENE 1,4 -DICHLOROBENZENE 3,3-DICHLORO- BENZIDINE DIETHYL PHTHALATE DIMETHYL PHTHALATE 2,4-DINITROTOLUENE 2,6-DINITROTOLUENE 1,2 -DIPHENYL - HYDRAZINE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 13 of 22 F,ACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NCO020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.) =POLLUTANT 'MAXIMUM & IL DISCHARGE " ' "AVERAGE DAILY' DISCFIARGE �ANALYTICAL.� METHOD ML/MDL Conc. Units J Mass., Units "' t Conc` , U,nIts;; 'Mass 'Units Nu(nber ;of Samples ; FLUORANTHENE FLUORENE HEXACHLOROBENZENE HEXACHLORO- BUTADIENE HEXACHLOROCYCLO- PENTADIENE HEXACHLOROETHANE INDENO(1,2,3-CD) PYRENE ISOPHORONE NAPHTHALENE NITROBENZENE N-NITROSODI-N- PROPYLAMINE N-NITROSODI- METHYLAMINE N -N ITROSO D (- PHENYLAMINE PHENANTHRENE PYRENE 1,2,4- TRICHLOROBENZENE Use this space (or a separate sheet) to provide information on other base -neutral compounds requested by the permit writer Use this space (or a separate sheet) to provide information on other pollutants (e.g., pesticides) requested by the permit writer .END -OF PART :D' REFER'TO�THE'APPLICAI`IO.N.OVERVIEW (PAGE'1) TO:DETERMIN'E WHICH OTHER PARTS OFfORM 2A YOU MUST COMPLETE,'. EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 14 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE ':SUPPLEMENTAL APPLICATION INFORMATION _ PARTE TOXICITY TESTING. DATA POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the facility's discharge points: 1) POTWs with a design flow rate greater than or equal to 1.0 mgd; 2) POTWs with a pretreatment program (or those that are required to have one under 40 CFR Part 403); or 3) POTWs required by the permitting authority to submit data for these parameters. • At a minimum, these results must include quarterly testing for a 12 -month period within the past 1 year using multiple species (minimum of two species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. • In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a toxicity reduction evaluation, if one was conducted. • If you have already submitted any of the information requested in Part E, you need not submit it again. Rather, provide the information requested in question EA for previously submitted information. If EPA methods were not used, report the reasons for using alternate methods. If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E. If no biomonitoring data is required, do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to complete. E.I. Required Tests. * POLLUTANT ANALYSIS SCAN RESULTS ATTACHED Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years. ❑ chronic ❑ acute E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one column per test (where each species constitutes a test). Copy this page if more than three tests are being reported. Test number: Test number: Test number: a. Test information. Test Species & test method number Age at initiation of test Outfall number Dates sample collected Date test started Duration b. Give toxicity test methods followed. Manual title Edition number and year of publication Page number(s) c. Give the sample collection method(s) used. For multiple grab samples, indicate the number of grab samples used. 24 -Hour composite Grab d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each. Before disinfection After disinfection After dechlorination EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 15 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NCO020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Test number: Test number: Test number: e. Describe the point in the treatment process at which the sample was collected. Sample was collected: f. For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both Chronic toxicity Acute toxicity g. Provide the type of test performed. Static Static -renewal Flow-through h. Source of dilution water. If laboratory water, specify type; if receiving water, specify source. Laboratory water Receiving water i. Type of dilution water. If salt water, specify "natural' or type of artificial sea salts or brine used. Fresh water Salt water j. Give the percentage effluent used for all concentrations in the test series. k. Parameters measured during the test. (State whether parameter meets test method specifications) pH Salinity Temperature Ammonia Dissolved oxygen I. Test Results. Acute: Percent survival in 100% effluent % % % LC50 95% C.I. % % % Control percent survival % % % Other (describe) EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 16 of 22 F/WILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NCO020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Chronic: NOEC % % % IC25 Control percent survival % % % Other (describe) m. Quality Control/Quality Assurance. Is reference toxicant data available? Was reference toxicant test within acceptable bounds? What date was reference toxicant test run (MM/DD/YYYY)? Other (describe) E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation? ❑ Yes ❑ No If yes, describe: EA. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information, or information regarding the cause of toxicity, within the past four and one-half years, provide the dates the information was submitted to the permitting authority and a summary of the results. Date submitted: / / (MM/DD/YYYY) Summary of results: (see instructions) END bF PART E. REFER TQ,THE.APPLICATION' OVERVIEW (PAGE '1), TO.DETERMINE WHICKOTHER PARTS OF FORM 2A YOU MUST'C.OMPLETE. EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 17 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE SUPPLEMENTAL APPLICATION `INFORMATION" PART F:INDUSTRIAL USERIDISCkiARGES ANp'RCRA/CERCLA WASTES., All treatment works receiving discharges from significant industrial users or which receive RCRA,CERCLA, or other remedial wastes must complete part F. GENERAL INFORMATION: F.I. Pretreatment program. Does the treatment works have, or is subject ot, an approved pretreatment program? ® Yes ❑ No F.2. Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of industrial users that discharge to the treatment works. a. Number of non-categorical.SlUs. 0 b. Number of CIUs. 1 SIGNIFICANT INDUSTRIAL USER INFORMATION: Supply the following information for each SIU. If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and provide the information requested for each SIU: F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages as necessary. Name: TEAM INDUSTRIES INC. Mailing Address: 3750 AIRPORT RD. ANDREWS, NC. 28901 F.4. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge. METAL FINISHING ELECTROPLATING AND ANCILLARY PROCESSES F.5. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's discharge. Principal product(s): GEAR CASES Raw material(s): ALUMINUM AND OTHER MATERIALS/METALS F.6. Flow Rate. a. Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons per day (gpd) and whether the discharge is continuous or intermittent. *90.000 gpd ( continuous or X intermittent) *PERMITTED FLOW b. Non-process wastewater flow rate. Indicate the average daily volume of non-process wastewater flow discharged into the collection system in gallons per day (gpd) and whether the discharge is continuous or intermittent. 25.000 gpd ( continuous or X intermittent) F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following: a., Local limits ® Yes ❑ No b. Categorical pretreatment standards ® Yes ❑ No If subject to categorical pretreatment standards, which category and subcategory? 40 CFR 433 EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 18 of 22 FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN: ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE F.8. Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e.g., upsets, interference) at the treatment works in the past three years? ❑ Yes ® No If yes, describe each episode. RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE: F.9. RCRA Waste. Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe? ❑ Yes ® No (go to F.12) F.10. Waste transport. Method by which RCRA waste is received (check all that apply): ❑ Truck ❑ Rail ❑ Dedicated Pipe FA 1. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units). EPA Hazardous Waste Number Amount Units CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION mi WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER: F.12. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities? ❑ Yes (complete F.13 through F.15.) ® No F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to origniate in the next five years). F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received). Include data on volume and concentration, if known. (Attach additional sheets if necessary.) F.15. Waste Treatment. a. Is this waste treated (or will be treated) prior to entering the treatment works? ❑ Yes ❑ No If yes, describe the treatment (provide information about the removal efficiency): b. Is the discharge (or will the discharge be) continuous or intermittent? ❑ Continuous ❑ Intermittent If intermittent, describe discharge schedule. END OF .PART F. REFER, TO THE APP:L-ICATION,OVERVIEW;(PAGE 1) TO DETERMINE WHICH .OTHER PARTS. OF FORM 2A YOU-MU,S;T COMPLETE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 19 of 22 Name and Address of Facility: Town of Andrews WWTP P.O. Box 1210 Andrews. N. C. 28901 Long Term Monitoring Plan Samples-EfFluent Earth EovlrooM60181 Sorvlces Michael Ladd 75 Bison Lane Murphy, NC 28906 Robbinsville Lab (828) 479-6428 Madison County Lab (828) 649-9250 Residence (828) 837-9543 Date: 3/10/2015 Sample Collector: MB Date and Time Sample Received: 2/17-20/15 See Date and Time Sample Collected: 2/16-20/15 C.O.C. Date and Time Sample Analyzed: < MHT Test 2/16-17/15 2/17-18/15 2/18-19/15 2/19-20/15 Report Limit Method Units BOD 35.1 19.2 13.6 17.3 2.0 SM5210B mg/L TOTAL SUSPENDED RESIDUE 25.0 22.8 16.3 18.3 1.0 SM2540D mg/L pH 6.7 6.6 6.5 6.3 0-14 SM4500H+ units AMMONIA NITROGEN 4.1 2.6 2.8 2.4 0.1 SM4500NH3F mg/L TEMPERATURE 9.4 8.2 6.4 6.3 0.1 SM2550B deg C CYANIDE TOTAL < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCASM4500 CN E mg/L CADMIUM < 0.001 < 0.001 < 0.001 < 0.001 0.0010 SCA -EPA 200.7 mg/L CHROMIUM < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA -EPA 200.7 mg/L COPPER 0.008 0.006 0.006 0.006 0.001 SCA -EPA 200.7 mg/L LEAD < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA -EPA 200.7 mg/L NICKEL < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L ZINC 0.042 0.032 0.032 0.033 0.010 SCA -EPA 200.7 mg/L SILVER < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA -EPA 200.7 mg/L ARSENIC < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L SELENIUM < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L MOLYBDENUM < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L MERCURY < 0.00020 < 0.00020 < 0.00020 < 0.00020 0.00020 SCA -EPA 200.7 mg/L OIL & GREASE 5.1 < 5.0 < 5.0 < 5.0 5.0 SCA-EPA1664A mg/L N.C. Laboratory ID# 352 Analyzed By: MB Note: pH and Temperature performed as Field Analyses by EES Lab Supervisor: Michael J. Ladd; EES-Robbinsville Certified By: Name and Address of Facility: Town of Andrews WWTP P:O, Box 1210 Andrews.N.C. 28901 Long Term Monitoring Plan Samples -Effluent Eorlh EoviroomWol Smicos Michael Ladd 75 Bison Lane Murphy, NC 28906 Robbinsville Lab (828) 479-6428 Madison County Lab (828) 649-9250 Residence (828) 837-9543 Date: 6/16/2014 Sample Collector: MB Date and Time Sample Received: 5/20-23/14 See Date and Time Sample Collected: 5/19-23/14 C.O.C. Date and Time Sample Analyzed: < MHT :Test 5119-20/14 5/20-21/14 5121-22/14 5/22-23/14 Report Limit Method Units ;BOD 23.1 16.6 16.9 15.4 2.0 SM5210B mg/L TOTAL SUSPENDED RESIDUE 14.3 8.1 8.5 8 1.0 SM2540D mg/L 1pH 6.6 6.1 6.1 6.0 0-14 SM4500H+ units AMMONIA NITROGEN 5.1 32 2.8 2.2 0.1 SM4500NH3F mg/L TEMPERATURE 17.8 16.5 17.2 18.2 0.1 SM2550B deg C CYANIDE TOTAL 0.006 0.006 0.015 0.023 0.005 scasM4500 CN E mg/L CADMIUM < 0.001 < 0.001 < 0.001 < 0.001 0.0010 SCA -EPA 200.7 mg/L CHROMIUM < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA -EPA 200.7 mglL COPPER 0.067 0.007 0.007 0.008 0.010 SCA -EPA 200.7 mg/L LEAD < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA -EPA 200.7 mg/L NICKEL < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L ZINC 0.037 0.037 0.036 0.043 0.030 SCA -EPA 200.7 mg/L SILVER < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA -EPA 200.7 mg/L ARSENIC < 0.010 < 0.010 < 0,010 < 0-010 0.010 SCA -EPA 200.7 mg/L SELENIUM < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L MOLYBDENUM < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L MERCURY < 0.00020 < 0.00020 < 0.00020 < 0.00020 0.00020 SCA -EPA 200.7 mg/L OIL & GREASE < 5.0 < 5.0 < 5.0 < 5.0 5.0 SCA-EPA1664A mg/L N.C. Laboratory ID# 352 Analyzed By: MB Nate: pH and Temperature performed as Field Analyses by EES Lab Supervisor Michael d_ Ladd; EES-Robbinsville Certified By: Name and Address of Facility: Town of Andrews WWTP P.O. Box 1210 Andrews. N.C. 28901 Lona Term Monitorina Plan SamDIes-Effluent Ear#6 EovifonmeoW Services Michael Ladd 75 Bison Lane Murphy, NC 28906 Robbinsville Lab (828) 479-6428 Madison County Lab (828) 649-9250 Residence (828) 837-9543 Date: 3/4/2013 Sample Collector: MB Date and Time Sample Received: 2/5-8/13 See Date and Time Sample Collected: 2/4-8/13 C.O.C. Date and Time Sample Analyzed: < MHT Test 2/4-5/13 2/5-6/13 2/6-7/13 217-8/13 Report Limit Method Units BOD 21.8 35.6 25.1 11.5 2.0 SM5210B mg/L TOTAL SUSPENDED RESIDUE 7.0 21.0 14.5 < 5.0 1.0 SM2540D mg/L pH 6.5 6.7 6.4 6.2 0-14 SM4500H+ units AMMONIA NITROGEN 1.4 2.1 1.6 0.9 0.1 SM4500NH3F mg/L TEMPERATURE 11.5 11.0 10.7 11.7 0.1 SM2550B deg C CYANIDE TOTAL < 0.0050 < 0.0050 < 0.0050 < 0.0050 0.0050 SCASM4500 CN E mg/L CADMIUM < 0.0010 < 0.0010 < 0.0010 < 0.0010 0.0010 SCA -EPA 200.7 mg/L CHROMIUM < 0.0050 0.012 0.0055 < 0.0050 0.0050 SCA -EPA 200.7 mg/L COPPER < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L LEAD < 0.0050 < 0.0050 < 0.0050 < 0.0050 0.0050 SCA -EPA 200.7 mg/L NICKEL < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L ZINC < 0.030 0.042 < 0.030" < 0.030 0.030 SCA -EPA 200.7 mg/L SILVER < 0.0050 < 0.0050 < 0.0050 < 0.0050 0.0050 SCA -EPA 200.7 mg/L ARSENIC < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L. SELENIUM < 0.010 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L MOLYBDENUM < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA -EPA 200.7 mg/L MERCURY < 0.00020 < 0.00020 < 0.00020 < 0.00020 0.00020 SCA -EPA 200.7 mg/L OIL & GREASE < 5.0 < 5.0 < 5.0 < 5.0 5.0 SCA-EPA1664A mg/L. N.C. Laboratory ID# 352 Analyzed By: MB Note: pH and Temperature performed as Field Analyses by EES Lab Supervisor: Michael J. Ladd; EES-Robbinsville Certified By: aENIZ 3r- ' `N ` Environmental Testing Solutions, Inc. March 25, 2016 Mr. Michael Ladd Earth Environmental Services 75 Bison Lane Murphy, NC 28906 RE: ETS PROJECT NUMBER: 11291 Dear Mr. Ladd: PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828)350-9368 Enclosed are toxicity test results for samples from the Andrews WWTP received by Environmental Testing Solutions, Inc. March 08 through March 10, 2016. Parameter Test Procedure EPA Method Final Code Number Result North Carolina Ceriodaphnia Chronic Effluent TGP3B Toxicity Procedure EPA -821-R-02-013 PASS (Ceriodaphnia Pass/Fail Toxicity Test) If this test was performed as an NPDES requirement or by Administrative Letter, please enter a P on the Effluent Discharge Monitoring Form (MR -1) for the collection date March 08, 2016 using the parameter code TGP3B. Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT -3) by April 30, 2016. If you have any questions concerning these results, please feel free to contact me. Sincerely, tm 1. Laboratory Director This report should not be reproduced, except in its entirety, without the written consent of Environmental Testing Solutions, Inc. The results in this report relate only to the samples submitted for analysis. North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600 South Carolina Certificate Number: Clean Water Act: 99053-001 r t 1 WN WJ qp rr� k � Environmental Testing Solutions, Inc. Effluent Aquatic Tnxicitv Rennrt Fnrm _ Phonp IT C'hrnn;v PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Date: March 25, 2016 Facility: Earth Environmental Services NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee Andrews NN'NVTP Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments: Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor: e. - - Project: 11291 Samples: 160308.15, 160310.13 Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center rStart date: End date: Start time: End time: Raleigh, NC 27699-1621 03-09-16 03-16-16 1348 0625 Sample Information Collection start date: Grab: Composite duration: Alkalinity (mg/L CaCO;): Hardness (mg1L CaCO3): Conductivity (µmhos/cm): Total residual chlorine (mg/L): Sample Temp. at Receipt (°C): Sample 1 Sample 2 Control 03-07-16 03-09-16 _- 13% 13% 13% 2425-h 25-h Control 7.38 7.17 30, 31, 32 -' 45, 42, 37 251 243 119. 152.156 < 0.10 <0.10 7.14 1.1 0.9 7.6 Test Information Treatment: Initial pH (SU): Final pH (SU): Initial DO (mg/L): Final DO (mg/L): Initial Temp. (°C): Final Temp. (°C): Organism Number Control OrLranisms 1 2 3 4 i f, 7 R 9 In 11 V) dart R-11 R-12 Stut Rwm,al l Rmml2 13% 13% 13% Control Control Control 7.38 7.17 7.08 7.51 7.31 7.08 7.16 7.13 7.10 7.16 7.14 7.05 7.6 7.6 8.0 7.8 7.7 7.8 7.8 7.9 7.9 7.8 7.8 7.8 25.0 25.3 24.8 24.8 24.8 24.9 25.1 25.1 25.1 25.1 24.9 25.0 Number of Young Produced 129 130129 131 27 128 131129 127 129 126 130 1 Adult Survival: (L)ive, (D)ead L L L L L L L L L L L L 288 Effluent Percentage 13 Treatment 7 Orunnicmc 1 d S 4 '7 v o in r r r) Number of Young Produced 132 35 36 35 34 34 35 133 133 32 134 36 34.1 Adult Survival: (L)ive, (D)ead I L L L L L L L I L I L L I L L -18.2 :n Kcauctlon Effluent Percentage= reatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan lumber of Young Produced Ault Survival: (L)ive, (D)ead %Reduc c Effluent Percentage= rearmenr w Organisms 1 % s 4 6 7 8 9 10 11 12 Mean lumber of Young Produced ldult Survival: (L)ive, (D)ead Redutti Effluent Percentage= 'reatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mr,n lumber of Young Produced Ault Survival: (L)ive, (D)ead .6 Rcdueiie Effluent Percentage= 'reatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean lumber of Young Produced tdult Survival: (L)ive, (D)ead KCaucllan Overall Analysis: Result: PASS LOEC: >13% NOEC: 13% ChV: >13% DWO-form AT -3 (8/91) Rev. 11195 Chronic Test Results Reproduction LOEC: Final Control Mortality (%): 0.0 % Control with 3rd Broods: 100 Control Reproduction CV: 5.5 48 Hour Mortality Method: Control: 0 of 17 IWC: 0 of 17 Significant.: No Final Mortality Sibmificant at: No concentration Reproduction Analyses Reproduction LOEC: >13% Reproduction NOEL: 13% Overall Method: Homoscedastic t Normal Distribution: Yes Method: Shapiro -Wilks Statistic: 0.950 Critical Value: 0.884 Equal Variances: Yes Method: F -Test Statistic: 1,323 Critical Value: 5.320 Non -Parametric Analysis (if applicable) Method: Effluent % Rank Sum Critical Sum 13% ; -T�— Page 1 of 1 • North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia (EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Control Bench Sheet Control #: Date: 03-09-16 Test Grouping Information: Test Organism Information: Facility Project # 6 Age: 5 kA4 UrN i 11?A� 4w6�nW1 A (►? 3 UOUrntLjo Replicate # 2 P X11 11 - Control �1 ai Daily Renewal Information: Organism Source: In-house Culture Test Initiation, renewal, feeding or termination Age: < 24 -hours old YWT Batch Source (culture board): 03.U"16 A Time Analyst Replicate # 1 2 3 4 5 6 7 8W91F12Culture board cup # �1 ai c{Q a Date and time organisms were born between: 0b-OR•110 01022 ID 130'L Average transfer volume: 0.0121 mL din Feeg it,/ Transfer bowl information: pH (S.U.): _.6 Temperature (°C): Z,� •� Day Date Test Initiation, renewal, feeding or termination SSW Batch Selenastrum Batch YWT Batch Location Incubator/Shelf Randomizing Template Time Analyst 0 03-09-16 initiation F d g 03-03-16 0'L''L\�1b �iS•Ib 2GZ �e� 1 03-10-16 din Feeg it,/ t _.6 L_ L 2 03- - 6 Renews Control, SSW Conductivity (µmhos/cm) (� q- , Number of broods present 3 03-12-16 Feeding F S `_yc� "f 3 0 1 v t 4 03-13-16 Feeding 77 *Hardness (mg/LCaCO3) 4 ir 5 03-14-16 Renewal Z3 ti ng +Analyst ide 66F 1 f r -1 *Temperature (°C) d�....o,-r TA'. —LS, 1 na li 6 03-15-16 Feed: 4pa _. Adult mortality I(L s �a « 1 a x 7 03-16-16 Termination S t a a. ai ,1 1 Chemical Analyses: Initiation Initial Final Renewal One Initial Final Renewal Two Initial Final Concentration Analyst 4 5 sw Iva NG 9 10 pH (S.U.) '} '1.1 b 1.31 %!q 7-09 'T,QS Dissolved oxygen (mg/L) _.6 L_ L 7,9 -7,9 79 Control, SSW Conductivity (µmhos/cm) (� q- , Number of broods present t ! P w s *Alkalinity (mg/L /LC 3 0 Lai .3 Z s emrg!" kt! dL 77 *Hardness (mg/LCaCO3) 4 ir 1-1-S 15 +Analyst ide 66F 1 f r -1 *Temperature (°C) d�....o,-r TA'. —LS, 1 na li -Z.�•G, 2N, is O ........................ ...o,. . "o,P..,aw.ca pcummw UL uLc UILLO UI Lest Im Omlon, renewal or termination by the analyst identified in the Daily Renewal Information table. Alkalinity and hardness performed by the analysts indentified on the test bench sheets and transcribed to this bench sheet. Survival and Renroduction Dntnr Day Observations Replicate number 1 2 3 4 5 6 7 8 9 10 11 12 2 Renewal One Adult mortality (L = Live, D = dead) I— �L_ L_ L , Number of broods present 2 Z L L Lai 7T 77 5 Numberofyoung produced ir 1-1-S 15 l� l� 1"` 12 IS 12 die Renewal Two Adult mortality I(L = Live, D = dead) Number of broods present 1 1 ( 1 l 1 I Number of young produced 1 (o t(4 110 110 13 7 Final Total young produced G �? \ �o Z \ AZ.. �i T, —LE 3` -tR Z7 p l t Z b �O Final adult mortality (L = Live, D = dead) X for 3rd Broods X I_untrof ficceprance Lrfteria: • of Male Adults (<_ 20%) • Adults having 3rd Broods (>_ 80%) I V Mortality (<_ 20%) Mean Offspring/Female (>_ 15 offspring/surviving female) % CV (< 40%) SOP AT17 - Fxhihit AT1 7 4 I -A6.,., t i — _1 n T74"' Page 1 of 1 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia (EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Test Bench Sheet Paired with Control #I Date: w-1nvto Client Earth Environmental Services NPDES # NCO020800 Facility Andrews WWTP outfall 001 Project # l �'L�1 \ County Cherokee Test Concentration (Chronic Limit) . 13.0% Dilution mL mL Total volume preparation: Sample Dilution water mL Samples were not aerated or treated unless otherwise noted on this form. Control, dilution water and test renewal information are included on the Control Bench Sheet indicated above. Chemical Analyses: Initiation Initial Final Renewal One Renewal Two Initial Final Initial -Final Concentration Analyst 50 §z 5W Ab Ah N 39 261 300 Chemical Analyses: Initiation Initial Final Renewal One Renewal Two Initial Final Initial -Final Concentration Analyst 50 §z 5W Ab Ah N pH (S.U.) -7-3 6 10 7. ) 7 7.13 7,og 7.0 Test Concentration Dissolved oxygen (mg/L) ,- iA -7,Q!ii,. % V.0 'rA4 Conductivity(µmhos/cm) a` ci t ; y` 4'r' - *Temperature ('C) 1S -O _M> - 100% pH(S.U,-1. .O / EFF ,,f . . h Dissolved oxygen (mg/L) Number of broods present Conductivity (µmhos/cm) *Total residual chlorine (mg/L) L0,10 tfl_h ? ' 1 Sample number sample 1 14 ,� Sample 2 03i,- . 13 -•v, 1--11,11- w 1,..,—, 11 .ny5-1 .,.0 1vuuue1 1 ly -cam,uury. , cuiperamres periormea at me time or rest initiation, renewal or termmatton by the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual Chlorine Bench Sheet and transcribed to this bench sheet. Survival and Reproduction Data (performed attest concentration) : Day Observations Replicate number 1 2 3 4 5 67 8 9 10 11 12 2 Renewal One Adult mortality (L = Live, D = dead) Number of broods present 1 i 5 Number of young produced �c+ , �+ Lj1 �� c G�1 RenewalTwo Adult mortality (L = Live, D = dead) Number of broods present \El LE l 11- t Number of young produced n 11 lot t I� is J Q A � S �f\ 16 11 I S 10 7 Final Total young produced 32 3S 3b 35INA W1 3S 33 33 3Z ,3A bLo Final adult mortality Fk__ (L = Live, D = dead)L-- --- "-- •• •••-•-- --•••o-"'I - -• ­­� •" ---- iii ncucm vuc la=y cl tuay al, amnp— were unuteu to me test concentration prior to Use With soft synthetic water and warmed to 2S.0± 1.0°Cin a warm water bath. Comments: Test Results and Statistical Analyses: M Test results Statistics % Mortality `n V /. Mean offspring 3il-1. per female % Reduction .yea? from control t -Stat or Rank -Sum I-�J 1 1 -Tailed Critical PASS or FAIL SOP AT12 - Exhibit AT12.2, revision 11-01-14 Concentration (%) Day Number of oung produced by replicate number Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test Average reproduction (offspring/female) Coefficient of variation (1y") Percent reduction from control ('Y,) 1 2 EPA-821-R-02-013, Method 1000.0 - North Carolina Modification 4 5 6 7 8 9 c ;1 11 12 Control 5 Quality Control 16 13 15 14 14 Verification of Data Entry, Calculations, and Statistical Analyses 17 12 15 12 16 Client: Andrews WWTP 28.8 5.5 Not applicable Test dates: March 09 - 16, 2016 16 14 16 Environmental Testing Solutions, Inc. Project number: 11291 14 17 12 Reveiwed by: j.,-" 14 14 14 Total Concentration (%) Day Number of oung produced by replicate number Survival M Average reproduction (offspring/female) Coefficient of variation (1y") Percent reduction from control ('Y,) 1 2 3 4 5 6 7 8 9 10 11 12 Control 5 13 16 13 15 14 14 14 17 12 15 12 16 100 28.8 5.5 Not applicable 7 16 14 16 16 13 14 17 12 15 14 14 14 Total 29 30 29 31 27 28 31 29 27 29 26 30 13% 5 15 18 17 17 18 19 16 18 15 15 19 17 100 34.1 4.0 -18.2 7 17 17 19 18 16 15 19 15 18 17 15 19 Total 32 35 36 35 34 34 35 33 33, 32 34 36 Dunnett's MSD value: 1.522 MSD = Minimum Significant Difference PMSD: 5.3 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10`x' percentile) = 13%. Upper PMSD bound determined by USEPA (90`1' percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001 b). USEPA. 2001x, 2001b. Final Report: Interlaboratory Variability Study of' EPA Short -tern Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 -Appendix. EPA -821-13-01-004 and EPA -821-13-01-005. US Environmental Protection Agency, Cincinnati, OH. Andreas 03-09-16 Transform: Untransfored_ 1 Tailed m Conc,% ,„ Mean .F N Mean,` Mean;, Min , .Max, GV% N t,stat., Critical MSD, .. a D -Control 28.833 1.0000 28.833 26.000 31.000 5.500 12 13 34.083 1.1821 34.083 32.000 36.000 4.046 12 -8.654 2.508 1.522 iAuxilia�y Test§ Statistic. " Critical' Skew.:'_ Kurt' .j Shapiro-Wiilk's Test indicates normal distribution (p > 0.01) 0.94975 0.884 -0.2526 -0.8427 F-Testindicates equal variances (p=0.65) � 5.31963 M In Hypotliesis Test.(1=tail,-0:01). - _`_ " :. " .. MSDu MSDp; ; ^`MSB ;,M$E, ' F=Prod `. ' .df I HomoscedastictTestindicatesnosignificant differences 1.52174 0.05278 165.375 2.20833 1.6E-08 1,22 Ai F : � ;31 Environmental Testing Solutions, Inc. _Ceriodaptinia:Suivival _._ and'Reprodtictibn iestSReproduction •_ Start Date: 3/9/2016 TestlD: CdPFCRNC Sample ID: Andrews WVVfP End Date: 3/16/2016 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR -Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: C. Conc:%° 2, 3 ,' 4... 5. " 6 Y ' 7 9'. 10 . D -Control 29.000 30.000 29.000 31.000 27.000 28.000 31.000 29.000 27.000 29.000 D -Control 26.000 30.000 13 32.000 35.000 36.000 35.000 34.000 34.000 35.000 33.000 33.000 32.000 13 34.000 36.000 Transform: Untransfored_ 1 Tailed m Conc,% ,„ Mean .F N Mean,` Mean;, Min , .Max, GV% N t,stat., Critical MSD, .. a D -Control 28.833 1.0000 28.833 26.000 31.000 5.500 12 13 34.083 1.1821 34.083 32.000 36.000 4.046 12 -8.654 2.508 1.522 iAuxilia�y Test§ Statistic. " Critical' Skew.:'_ Kurt' .j Shapiro-Wiilk's Test indicates normal distribution (p > 0.01) 0.94975 0.884 -0.2526 -0.8427 F-Testindicates equal variances (p=0.65) 1.32271 5.31963 Hypotliesis Test.(1=tail,-0:01). - _`_ " :. " .. MSDu MSDp; ; ^`MSB ;,M$E, ' F=Prod `. ' .df I HomoscedastictTestindicatesnosignificant differences 1.52174 0.05278 165.375 2.20833 1.6E-08 1,22 Treatments vs D -Control Andrews 03-09-16 M h >- Environmental Testing Solutions, Inc. 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828)350-9368 Whole Effluent Toxicity Chain -of -Custody Form Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee Andrews WWTP Purchase order: Species: Ceriodaphnia dubia Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP3B Sample information: (to be completed by sample collector) Composite sample: r r/, U Start date: M 1 6 `•Y End date: Number of samples per hour: Time: 2,,'Q 3 Av'%. Time: G�- Sample location: Volume collected for testing: Number of containers filled for testing: Method of transport to laboratory: Chilled during collection? Comments: If chilled, specify temperature: Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: Print Signature Dale and time Relinquished by: k y Print signature Dale and time Relinquished by: Print signature Date and time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS y: r / 3 Print signature Date and time Custody seals intact?: = Yes No Not used Samples received in good condition?: = Yes No T4 f"' Tracking number: Comments: Received by: K,LAIL WKV W --% {Print Signature Date and time d Received by: s Print Signature Dale and time Received at ETS bv: �� Vim- 03, t7� ij0 �(2 Print Jlgnaturc Uale and trate Sample temperature upon receipt at ETS (dC): Total residual chlorine upon receipt at ETS: (DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Absent Project number: I UA I Sample number: %U0.30A Its 7-- -q Environmental Testing Solutions, Inc. 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Whole Effluent Toxicity Chain -of -Custody Form Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee Andrews WWTP Purchase order: Species: Ceriodaphuia dubia Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP3B Imple mtormation: (to oe completea Dy sample couector) Composite sample: ,r� MI- -110 mple location: C CS`' �l� `L tl �,l Start date: 3 Time: Vel S R Volume collected for testing: 1 l.r e'l End date: Time: Number of containers filled for testing: Number of samples per hour: 1 -e- Method of transport to laboratory: Chilled during collection? 1.l !e S Comments: If chilled, specify temperature: 1 " Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: lv-�l 31161,114n Print Signature Date and time Relinquished by: Receival by: f ti Print Signature Dale and time Print Signature Date and time P Relinquished by: Received by: jX C Print Signature Date and time Print Signature Dale and time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: R -:WC)IIL�- L I I 132 Print Signature Date and time Custody seals intact?: = Yes No Not used Samples received in good condition?: Ft Yes No Tracking number: Ji Comments: Received at ETS bv: ,1� ►'K�--13 � 03.►o�N� 2� rnm aignaure -le ano ume Sample temperature upon receipt at ETS (°C): Q •� �Ci Total residual chlorine upon receipt at ETS: (DPD Presence/Absense Indicator, MDL = 0.10 ing/L) Present Absent Project number: MAI Sample number: 1410310'13 Ov€ q C ? rK' ,;J Environmental Testing Solutions, Inc. 1.14 1.12 1.10 1.08 1.06 1.04 1.02 2.5 2.0 z 1.5 e� �j 1.0 .� 0.5 r 1.4 1.3 1.2 1.1 1.0 0.9 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart USEPA Control Limits (:I:: 2 Standard Deviations) USEPA Warning and Control Limits (75th and 90th Percentile CVs) Laboratory Warning and Control Limits (10th and 25th Percentile CVs) .......•..........................•..•.•..•..............•..................................... 081 0� -VA 0� 1a OA ,A Oti �A �� 03 15 0� �� 0J N$ OS ,� 09 ,� �a ,5 OA �� �5 ,5 06 ,-0� �5 0�'0 1ti `6 ,ti, 0�� Otis Otis 0� Test date • 7 -day IC25= 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. — — Central Tendency (mean IC25) — Warning Limits (mean IC25 Sato or SA75) q,,w ... mm.e, ......... Control Limits (mean IC25± SA.Zs1 SA.90' or 2 Standard Deviations) 0` s-- grpp I r PO Box 7565 Asheville NC 28802 Phone: (828) 350-9364 Fax: (828)350-9368 ti. E Environmental Testing Solutions, Inc. June 27, 2016 Mr. Michael Ladd Earth Environmental Services 75 Bison Lane Murphy, NC 28906 RE: ETS PROJECT NUMBER: 11545 Dear Mr. Ladd: Enclosed are toxicity test results for samples from the Andrews WWTP received by Environmental Testing Solutions, Inc. June 14 through June 16, 2016. Parameter Test Procedure EPA Method Final Code Number Result North Carolina Ceriodaphnia Chronic Effluent TGP3B Toxicity Procedure EPA -821-R-02-013 PASS (Ceriodaphnia Pass/Fail Toxicity Test) If this test was performed as an NPDES requirement or by Administrative Letter, please enter a P on the Effluent Discharge Monitoring Form (MR -1) for the collection date June 14, 2016 using the parameter code TGP313. Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT -3) by July 31, 2016. If you have any questions concerning these results, please feel flee to contact me. Sincerely, Jimumner Laboratory Director This report should not be reproduced, except in its entirety, without the written consent of Environmental Testing Solutions, Inc. The results in this report relate only to the samples submitted for analysis. Nort11 Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600 South Carolina Certificale Number: Clean Water Act: 99053-001 PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828)350-9368 Datta: June 27, 2016 Facility: Earth Environmental Services NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee Andrews MVWTP Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments: Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor; r A • Project: 11545 Samples: 160614.07, 160616.07 Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center Start date: End date: ! Start time: End time: Raleigh, NC 276994621 06-15-16 06-22-16 r 1230 0703 Sample Information Collection start date: Grab: Composite duration: Alkalinity (mgtL CaCO;): Hardness (mg/L CaCO,): Conductivity (pntltos/cm): Total residual chlorine (mg/L): Sample Temp. at Receipt ("C): Sample 1 Sample 2 Control 06-13-16 06-15-16 - 13% 13% 13% 24-h 24.5 -It -' 7.55 7.67 30 7.67 - 40 260 234 1st. tss. lsc <0.10 i - 1.2 0.7 7.8 i Environmental Testing Solutions, Inc. Effluent Aauatic Toxicitv Report Form - Phase II Chronic Cerindnnhnin dnhin PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828)350-9368 Datta: June 27, 2016 Facility: Earth Environmental Services NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee Andrews MVWTP Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments: Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor; r A • Project: 11545 Samples: 160614.07, 160616.07 Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center Start date: End date: ! Start time: End time: Raleigh, NC 276994621 06-15-16 06-22-16 r 1230 0703 Sample Information Collection start date: Grab: Composite duration: Alkalinity (mgtL CaCO;): Hardness (mg/L CaCO,): Conductivity (pntltos/cm): Total residual chlorine (mg/L): Sample Temp. at Receipt ("C): Sample 1 Sample 2 Control 06-13-16 06-15-16 - 13% 13% 13% 24-h 24.5 -It -' 7.55 7.67 30 7.67 - 40 260 234 1st. tss. lsc <0.10 <0_10 - 1.2 0.7 7.8 i Test Information Treatment: Initial pH (SU): Final pH (SU): Initial DO (ing/L): Final DO (mg/l.): Initial Temp. ("C): Final Temp. ("C): Organism Number ControlOreanisms 1 2 3 4 5 6 7 R 9 10 11 17 Stan Rmcm1l I Rene al 2 Sian Rmrnal l R.12 13% 13% 13% Control Control Control 7.55 7.67 7.50: 7.67 7.75 7.81 7.70 7.77 7.61 7.71 7.86 7.56 7.8 7.8 7.8 i 7.8 7.9 7.7 7.9 8.0 7.8 7.9 8.0 7.9 24.9 25-0 24.8 24.8 24.7 24.7 252 25.1 25.0 24.7 1 24-7 24.7 Number of Young Produced 126 29 25 25 29 25 26 26 27 28 26 27 28.9 Adult Survival: (L)ive, (D)ead I L L L L L L L L L L L L 26.6 Effluent Percentage 13% Trentment2 Ortranicmc 1 7 d S A 7 2 0 In 17 17 Number of Young Produced 132 29 29 30 27 29 30 28 28 27 29 29 28.9 Adult Survival: (L)ive, Mead I L L L L L L L L L L L L -8.8 -r. RcUM- Effluent Percentage= reatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 UeaR lumber of Young Produced -H Ault Survival: (L)ive, (D)ead I c6 Rcdunis Effluent Percentage= "reatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Nieaa (umber of Young Produced AWL Survival: (L)ive, (D)ead 3. Reductic Effluent Percentage= 'reatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 �lcm (umbel- of Young Produced ,dull Survival: (L)ive, (D)ead %Redunic Effluent Percentage= •reatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 ' dlean lumber of Young Produced Ault Survival: (L)ive, (D)ead Overall Analysis: Result: PASS LOEC: >13% NOEC: 13% ChV: >13% DIYO form AT -3 (8/91) Rev. 11195 Chronic Test Results Reproduction LOEC: Final Control Monality (%): 0.0 % Control with 3rd Broods: 100 Control Reproduction CV: 5.4 48 Hour Mortality Method: Control: 0 of 12 IWC: 0 of 12 Significant?: No Final Mortality Significant at: No concentration Reproduction Analyses Reproduction LOEC: >13% Reproduction NOEC: 13% Overall Method: Homoscedastic t Normal Distribution: Yes Method: Shapiro -Min.'s Statistic: 0.938 Critical Value: 0.884 Equal Variances: Yes Method: F -Test Statistic: 1.096 Critical Value: 5.320 Non -Parametric Analysis (if applicable) k4ethod: Effluent 9b Rank Sum Critical Sum 13% • North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia alubia Page 1 of 1 • (EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Controlx Bench Sheet Control #: Date: 06-15-16 Test Grouping Information: Test Organism Information: Daily Renewal Information: Day Date 0 06-15-16 1 06-16-16 2 06-17-16 3 05-18-16 4 06-19-16 5 06-20-16 6 06-21-16 7 1 06-22-16 Organism Source: Facility Project # 6 Source (culture board): 6"11-041 5 tpmweiZul1 Culture board cup # 4 F Vt,\ N b 3 g l.Y tL 1 S pH (S.U.): -'j • A�t Temperature ('Q: -tS•` 2 1- Control` 7,7/ Daily Renewal Information: Day Date 0 06-15-16 1 06-16-16 2 06-17-16 3 05-18-16 4 06-19-16 5 06-20-16 6 06-21-16 7 1 06-22-16 Organism Source: In-house Culture Age: a 24 -hours old Source (culture board): 6"11-041 Replicate # 1121314 5 6: 7 8 9 10 'il 12 Culture board cup # Sig 110 it tj tF t li Date and time organisms were born between: Otr�s-Ile OWE• `m mlo Average transfer volume: 0.0121 mL Transfer bowl information: pH (S.U.): -'j • A�t Temperature ('Q: -tS•` SSW Batch Selenastrum ywr Location Batch Batch Incubator/Shelf 6--%,L46 oS-2�•►� o5-vq-►16. I -M-ls Chemical Analyses: Initiation Renewal One Renewal Two % Mortality (<_ 20%) Initial Final Initial Final Initial Final Concentration Analyst 8 lyb 10 li - 2 pH (S -U.) -1 -VI 7,7/ 7.7S' '� • ' , ']'. Dissolved oxygen (mg/L) -1. 7,8 7.1 5-0 7 It Conductivity (µmhos/un) Control, SSW ) S (L = Uve, D = dead) I sst 5 4 y *Alkalinity (mg/L CaCO3) od•x7}ti z� *Hardness (mg/LCaCO3) y() Number of broods present ZLl *Temperature (°C) 'Analyst Z.� Z-4•-1•1 Z-�` 1. Zl•� %`�. identified for each day,performed pH; dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table. Alkalinity hardness rLZ and performed by the analysts indentified on the test bench sheets and transcribed to this bench sheet - Survival and Renrodurtinn nnfv"- 1t v L -L Day observations Replicate number O 67. % Mortality (<_ 20%) 1 2 1 3 4 5 1 6 F 7 8 9 10 li 12 2 Adult mortality Renewal One (L = Uve, D = dead) � � � � Number of broods present ZLl L2_Z Z rLZ 5 Number.of young produced 1t v L -L (Z lZ IS Renewal Two Adult mortality (L=Live, D=dead) �-- L_ Lr I_ ` L Number of broods presentLl L% Lk` Ll L I Number of young produced t �p l t I (o t J l j 111A q7 Final Total young produced �- Zr1 15 Z-5 Z`l 1S 16 Zb -M UZ '46 2 l Final adult mortality (L= Live, D= dead) . L X for 3rd Broods X % of Male Adults (5 20%) r % Adults having 3rd Broods (>_ 8091.) O 67. % Mortality (<_ 20%) Mean Offspring/Female (>t 15 offspring/surviving female) .4"o %CV (<40%) 5•�Z SOP AT12 - Exhibit AT12.3,.revision 11-01-14 Concentration (%) Day ,:,' Ceriodaphttia (labia Chronic Whole Effluent Toxicity Test Average reproduction (offspring/female) v Coefficient of variation (%) Percent reduction from control ('%) 1 2 dui J EPA -821-R-02-013, Method 1000.0 - North Carolina Modification 5 6 7 8 9 10 11 12 Control 5 12 13 II 11 13 12 II 12 IS 12 13 Quality Control 100 26.6 5.4 Not applicable 7 14 16 Verification of Data Entry, Calculations, and Statistical Analyses 14 16 13 15 14 12 16 13 14 Total 26 29 Client: Andrews WWTP 25 Environmental Testing Solutions, Inc. 25 Test dates: June 15 - 22, 2016 26 27 28 26 Project number: 11545 13% 5 16 15 Reveiwed by: 5!n!=� Concentration (%) Day Number oryoun roduced by replicate number survival ('M,) Average reproduction (offspring/female) v Coefficient of variation (%) Percent reduction from control ('%) 1 2 3 4 5 6 7 8 9 10 11 12 Control 5 12 13 II 11 13 12 II 12 IS 12 13 13 100 26.6 5.4 Not applicable 7 14 16 14 14 16 13 15 14 12 16 13 14 Total 26 29 25 25 29 25 26 26 27 28 26 27 13% 5 16 15 12 15 11 11 15 13 12 12 12 15 100 28.9 4.8 -8.8 7 16 14 17 15 16 18 15 15 16 15 17 14 Total 32 29 29 30 27 29 30 28 28 27 29 29 Dnnnett'S MSD value: 1.445 MSD = Mininuun Significant Dirfcrcnce I'M SD: 5.4 PMSD = Percent Mininutm Significant Difference PMSD is a measure of test precision. The PMSD is the miniintun percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity lest. Lower PMSD bound determined by USEPA (10'x' percentile) = 13%. Upper PMSD bound determined by USEPA (90111 percentile) = 47%. Lower and upper PMSD bounds were determined from the l Olh and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Vanab.ility Study (tJSEPA, 2001 a: USEPA, 2001 b). _ USEPA. 200 la, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent'roxicity'I'cst Methods, Volumes I and 2 -Appendix. EPA -821-13-01-004 and L'PA-821-13-01-005. US Environmental Protection Agency, Cincinnati, OI -I. Anthems 06-15-M A, 3��:& Lj i .< Environmental Testing Solutions, Inc. Ceriodaplinfa Survival and'Reproduction Test -Reproduction Start Date: 6/15/2016 Test ID: CdPFCRNC Sample ID: Andrews WVVTP End Date: 6/22/2016 Lab lD: ETS-Envir. Testing Sol. Sample Type: DMR -Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: ; D -Control 26.000 29.000 25.000 25.000 29.000 25.000 26.000 26.000 27.000 28.000 D -Control 26.000 27.000 13 32.000 29.000 29.000 30.000 27.000 29.000 30.000 28.000 28.000 27.000 13 29.000 29.000 -x i ransrorm, IJntranstormed _ : fi=Tailed Cont % Mean N Mean Mean Mm ;_ ,Max"/o N t -Stat Critical: MSD- rol 26.583 1.0000 26.583 25.000 29.000 5.430 12 13 28.917 1.0878 28.91.7 27.000 32.000 4.769 12 -4.049 2.508 1.445 AuxiliaryTestsCrttiCal Shapiro--WilWs Test indicates normal distribution (p > 0.01) 0.93804 0.884 0.62647 -0.1212 F -Test indicates equal variances (p = 0.88) 1.09562 5.31963 Treatments vs D -Control no significant differences 1A4544 0.05437 32.6667 1.99242 5.4E-04 1, Andi•eirs 06-15-16 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. wnole mlluent Toxicity Chain -of -Custody Form Facility: Earth Environmental Services, Inc. NPDES # NCO020800 Pipe #: 001 County: Cherokee Andrews WWTP Purchase order: Species: Ceriodaphida dubia Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP31B Sample information: Composite sample: S�KN���: Sample location: r Startdate: -'. Time: C,,- Volume collected for testing: tsar I End date: (0- C L(4WO Time: Odo-4-vu Number of containers filled for testing: t •'t`C. Number of samples per hour: 1 S �,r, tp (�a_� S� u L Method of transport to laboratory: pr "m Aba-m Chilled during collection? y S Comments: If chilled, specify temperature: f " Triple rinse sample container with sample before filling. Completely fill the sample container with no air space Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: Sample collected by ttU nt: wn,pteteu oy sample couector anu lacutty personnel) Ir"� b� - • ; etc �c Print $ ^nature _ Date and time Relinquished by: Print Signature Date and time Received bv_ - -^ ..V.— ate and Wnc Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: 17 t 'Y -�W 1 &711 q 7 16 Q\ --all 1SoS Prim Signature Date and time Custody seals intact?: u Yes No Not used Samples received in good condition?: Yes No Tracking number: 0 P, Comments: Received at ETS by: / S�KN���: G to j - -^ ..V.— ate and Wnc Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: 17 t 'Y -�W 1 &711 q 7 16 Q\ --all 1SoS Prim Signature Date and time Custody seals intact?: u Yes No Not used Samples received in good condition?: Yes No Tracking number: 0 P, Comments: Received at ETS by: / ti S�KN���: G to tsar I Print VSignature Dal. and time Sample temperature upon receipt at ETS (°.C): t •'t`C. Total residual chlorine upon receipt at ETS: = Full (DPD Presence/Absense Indicator. MDL — 0. l0 mg/L) pr "m Aba-m 54 Projectnumber:1t Sample number: 1(00t AUT ti Whole Effluent Toxicity Chain -of --Custody Form Facility: - Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee Andrews WWTP Purchase order: Species: Ceriodaphitia dabfa Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP3B Sample information, (to be completed by sample collector) i Composite sample: Sample location: , Start date: Time: 060 1%V Volume collected for testing: ;t J End date: i0[[ G r( V-7 Time: 0931 A/ Number of containers tilled for testing: Number of samples per hour: j d.,,,1(e,75O V Q : A Method of transport to laboratory: J- Chilled during collection?�/ eS Comments: If chilled, specify temperature: / D Triple rinse sample container with sample before filling. Completely fill the sample container with'no air space. s Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) i Satuple collected by:=3�tv-\ Prinlsign.t= Datcand time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: '., � Received at ETS by: Fni C �U[' Ob•Id•tlo L. 1 l �3P'1 ��Maw 1,643 Print Sigranvc Date and lime Print sigtawrc Date and tine Custody seals intact?: = Ycs \0 Nei used Samples received in good condition?; = Yes Na Traclting number: Comments: Sample temperature upon receipt at ETS (°C): v•, I, C Total residual chlorine upon receipt at ETS: (DPD PreseneeiAbsense Indicator. MDL = 0.10 mg1L) Ptcsent absent Project number: Sample number: ka Q(p ((o.m- - 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. Whole Effluent Toxicity Chain -of --Custody Form Facility: - Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee Andrews WWTP Purchase order: Species: Ceriodaphitia dabfa Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP3B Sample information, (to be completed by sample collector) i Composite sample: Sample location: , Start date: Time: 060 1%V Volume collected for testing: ;t J End date: i0[[ G r( V-7 Time: 0931 A/ Number of containers tilled for testing: Number of samples per hour: j d.,,,1(e,75O V Q : A Method of transport to laboratory: J- Chilled during collection?�/ eS Comments: If chilled, specify temperature: / D Triple rinse sample container with sample before filling. Completely fill the sample container with'no air space. s Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) i Satuple collected by:=3�tv-\ Prinlsign.t= Datcand time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: '., � Received at ETS by: Fni C �U[' Ob•Id•tlo L. 1 l �3P'1 ��Maw 1,643 Print Sigranvc Date and lime Print sigtawrc Date and tine Custody seals intact?: = Ycs \0 Nei used Samples received in good condition?; = Yes Na Traclting number: Comments: Sample temperature upon receipt at ETS (°C): v•, I, C Total residual chlorine upon receipt at ETS: (DPD PreseneeiAbsense Indicator. MDL = 0.10 mg1L) Ptcsent absent Project number: Sample number: ka Q(p ((o.m- - r Environmental Testing Solutions, Inc. 1.14 1.12 1.10 1.08 1.06 1.04 1.02 'r_XI U 2.0 1.5 �n U 1.0 1-4 0.5 _C 1.4 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart USEPA Control Limits 2 Standard -Deviations) ............................................................ .............................................................................................. USEPA Warning and Control Limits (75th and 9Ott, Percentile CVs) ................................................................ .............................. J 1.3 Laboratory Warning and Control Limits (10th and 25th Percentile CVs) 1-2 1.0 0.9 .........................................;............................. 0.8 .5X5 O�A3 XONOSA5 X06 pq �o� ,�•�Q$ pA toq ,cs 150pbp9r%o, �b �oL pq �oL pq lob p��oa p��0a p5 SO -e" Test date 7 -day IC,, = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. — — Central Tendency (mean IC20 — — - Warning Limits (mean IC,5 f SA 10 or SA,,) 'Yw.4wrM b • • • • • • • • • Control Limits (mean IC f S _ S z�_ �.z>> a9o� or � Standard Deviations) {= F 4 r Environmental Testing Solutions, Inc. 1.14 1.12 1.10 1.08 1.06 1.04 1.02 'r_XI U 2.0 1.5 �n U 1.0 1-4 0.5 _C 1.4 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart USEPA Control Limits 2 Standard -Deviations) ............................................................ .............................................................................................. USEPA Warning and Control Limits (75th and 9Ott, Percentile CVs) ................................................................ .............................. J 1.3 Laboratory Warning and Control Limits (10th and 25th Percentile CVs) 1-2 1.0 0.9 .........................................;............................. 0.8 .5X5 O�A3 XONOSA5 X06 pq �o� ,�•�Q$ pA toq ,cs 150pbp9r%o, �b �oL pq �oL pq lob p��oa p��0a p5 SO -e" Test date 7 -day IC,, = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. — — Central Tendency (mean IC20 — — - Warning Limits (mean IC,5 f SA 10 or SA,,) 'Yw.4wrM b • • • • • • • • • Control Limits (mean IC f S _ S z�_ �.z>> a9o� or � Standard Deviations) September 23, 2016 Mr. Michael Ladd Earth Environmental Services 75 Bison Lane Murphy, NC 28906 RE: ETS PROJECT NUMBER: 11720 Dear Mr. Ladd: PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Enclosed are toxicity test results for samples from the Andrews WWTP received by Environmental Testing. Solutions, Inc. September 06 through September 08, 2016. Parameter Test Procedure EPA Method Final Code Number Result North Carolina Ceriodaphnia Chronic Effluent TGP3B Toxicity Procedure EPA -821-R-02-013 PASS (Ceriodaphnia Pass/Fail Toxicity Test) - . If this test was performed as an NPDES requirement or by Administrative Letter, please enter a P on the Effluent Discharge Monitoring Form (MR -1) for the collet_tion date September 06, 2016 using the parameter code TGP3B. Additionally,, please sign and submit the original DWQ Aquatic Toxicity Form (AT -3) by October 31, 2016. If you have any questions concerning these results, please feel free to contact me. Sincerely, QtUmrner Laboratory Director This report should not be reproduced,'except in its entirety, without the written consent of Environmental Testing Solutions, Inc. The results in this report relate only to the samples submitted for analysis North Carolina Certificate Nwnbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600 South Carolina Certificate Number: Clean Water Act: 99053-001 0. aa , Environmental Testing Solutions, Inc. September 23, 2016 Mr. Michael Ladd Earth Environmental Services 75 Bison Lane Murphy, NC 28906 RE: ETS PROJECT NUMBER: 11720 Dear Mr. Ladd: PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Enclosed are toxicity test results for samples from the Andrews WWTP received by Environmental Testing. Solutions, Inc. September 06 through September 08, 2016. Parameter Test Procedure EPA Method Final Code Number Result North Carolina Ceriodaphnia Chronic Effluent TGP3B Toxicity Procedure EPA -821-R-02-013 PASS (Ceriodaphnia Pass/Fail Toxicity Test) - . If this test was performed as an NPDES requirement or by Administrative Letter, please enter a P on the Effluent Discharge Monitoring Form (MR -1) for the collet_tion date September 06, 2016 using the parameter code TGP3B. Additionally,, please sign and submit the original DWQ Aquatic Toxicity Form (AT -3) by October 31, 2016. If you have any questions concerning these results, please feel free to contact me. Sincerely, QtUmrner Laboratory Director This report should not be reproduced,'except in its entirety, without the written consent of Environmental Testing Solutions, Inc. The results in this report relate only to the samples submitted for analysis North Carolina Certificate Nwnbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600 South Carolina Certificate Number: Clean Water Act: 99053-001 PO Box 7565 Asheville, NC 28802 t. iq Phone: (828) 350-9364 N - Fax: (828) 350-9368 Environmental Testing Solutlons, Inc. Effluent Aquatic Toxicity Report Form - Phase I1 Chronic Ceriodaphnia diubia Date: September 23, 2016 Facility: Earth Environmental Services NPDES #: NC - .0020800 Pipe #: 001 County: Cherokee Andrews WWTP Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments: Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor:Dutbi� Project: 11720 Samples: 160906.06, 160908.07 Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center Start date: End date: Start time: End time: Raleigh, NC 27699-1621 09-07-16 09-14-16 1010 0802 Sample Information Collection start date: Grab: Composite duration: Alkalinity (mg/L CaCO3): Hardness (mg/L CaCO3): Conductivity (µmhos/cm): Total residual chlorine (mg/L): Sample Temp. at Receipt (°C): Sample 1 Sample 2 Control 09-05-16 09-07-16 Rcnnval 2 13% 13% 13% 24.5-h 24.5-h - 7.67 34 E _ } 9M 40 530 385 161.160.1 9 <0.10 <010 7.81 4.5 0.9 7.8 Test Information Treatment: Initial ptI (SU): Final pH (SU): Initial DO (mg/Ly Final DO (mg/L): Initial Temp. (°C): Final Temp. (°C): Organism Number Control Oreanisms 1 2 I S ( 7 9 O in t t ii Start Rcn W t Rcnn\a12 Stan Rrncnal 1 Rcnnval 2 13% 13% 13% Control Control Control 7.67 7.50 7.59 7.52 7.49 7.53 7.78 7.69 7.63 7.63 7.81 7.64 7.9 7.8 7:9 7.9 7.8 7.9 7.9 8.0 7.9 7.9 8.0 7.9 24.9 24.7 24.9 24.8 24.9 24.8 25.0 24.9 25.1 24.8 24.8 25.0 Number of Young Produced 130 26 27 28 28 29 28 27 27 129 29 30 29 Adult Survival: (L)ive, (D)ead I L L L L L L L L L LL L L 28.2 Effluent Percentage 13 Treatment 2 Oranniemc 1 7 Z d c A 'r o n rn 11 1.9 Number of Young Produced 132 29T26 0.0 128 128 27 29 27 29 27 26 30 28.2 Adult Survival: (L)ive, (D)ead I LLL. 0 I L I L I L L L L L L L L 0.0 I KWacuo. Effluent Percentage= reatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean lumber of Young Produced Ault Survival: (L)ive, (D)ead oa Rcducti, Effluent Percentage= rcatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 hlaan lumber of Young Produced Ault Survival: (L)ive, (D)ead Effluent Percentage= reatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 M.n lumber of Young Produced tdult Survival: (L)ive, (D)ead % Rcductic Effluent Percentage= Ireatment6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 MG lumber of Young Produced ,dult Survival• (L)ive, (D)ead Rcductic Overall Analysis: Result: PASS LOEC: >13% NOEC: 13% ChV: >13% DWO form AT -3 (8191) Rev. 11195 Chronic Test Results Reproduction LO EC: Final Control Mortality (%): 0.0 % Control with 3rd Broods: 100 Control Reproduction CV: 4.5 48 Hour Mortality Method`. Control: 0 of 12 IWC: 0 of 12 Significant?-. No Final Mortality Significant at: No concentration Reproduction Analyses Reproduction LO EC: >13% Reproduction NOEC: 13% Overall Method: Homoscedastic t Normal Distribution: Yes Method`. Shapiro-Wilk's Statistic: 0:933 Critical Value: 0.884 Equal Variances: Yes Method: F -Test Statistic: 1.906 Critical Vallee: 5.320 Non -Parametric Analysis,017applicable) Mcthod: Effluent % Rank Sum Critical Stan-- um13% 13% North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia Page 1 of 1 (EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Control Bench Sheet _ Test Grouping Information: Test Organism Informati 1 Daily Renewal Information: oControl #: �Date: 09-07-16 n. Organism Source: (culture board): -house Culture 24 -hours old Culture board cup # 4. Date and time organisms were 00`-T%Llo 0100 r0 two born between: Average transfer volume: 0.03 mL Transfer bowl information: IPH (S.U.): "I - (°c)` -Lt.1 Day Facility Project # 6 MVV%-jtl.Le lX"1Ito 5 t.1 i la l 4 c�IF�SIUe ICI -1 Time Analyst Initiation/Feeding m%0d0. Feeding Renewal Feeding3 C Fg0Ot{Ol2/FedingeA Rw12/Fe e J6 Temin(on7 o 0 1 2 4 5 09-07-16 09-08-16 09-09-16 09-10-16qJ�Feeding 09-11-16 09-12-16 09-13-16Al 09-14-16 P-2 Batch 8'�0 A9 �- Batch oc IF It, r ontrol Template gl.LJr~ $ Daily Renewal Information: oControl #: �Date: 09-07-16 n. Organism Source: (culture board): -house Culture 24 -hours old Culture board cup # 4. Date and time organisms were 00`-T%Llo 0100 r0 two born between: Average transfer volume: 0.03 mL Transfer bowl information: IPH (S.U.): "I - (°c)` -Lt.1 Day Date Test Initiation, renewal, feeding or term nation SSW Bates 7, 91 6y 4 Dissolved oxygen (mg/L) Selenastrum YWT Location Randomizing Time Analyst Initiation/Feeding m%0d0. Feeding Renewal Feeding3 C Fg0Ot{Ol2/FedingeA Rw12/Fe e J6 Temin(on7 o 0 1 2 4 5 09-07-16 09-08-16 09-09-16 09-10-16qJ�Feeding 09-11-16 09-12-16 09-13-16Al 09-14-16 C&16 0% Ac, 4- - NI? b r Batch 8'�0 A9 �- Batch oc IF It, r Incubator/Shelf Z�\ 'a` -T Template gl.LJr~ $ Chemical Analyses: Initiation Renewal One Renewal Two Initial Final Initial Final Initial Final Concentration Analvst niti Ar I I_._ Day PH (S.U.) Replicate number . 7.63 7, 91 6y 4 Dissolved oxygen (mg/L) 'J q 7 8 7 f 7, Control, SSW��4 Conductivity (µmhos/cm)(� 12 2 Renewal One a rrsb �,, m L_ Alkalinity (mg/LCaCO) ` z t, I`, ti- L `Hardness (mg/L CaCO,) L I I-- L_ 5 Renewal Two `Analyst Temperature (°C) -114�ty ��- � 0% 1�• � 1�- � V •� - identified far each day, performed pH, dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal information table. Alkalinity and hardness performed by the analysts indentified L- IS on the test bench sheets and transcribed to this bench sheet. Survival and Reproduction Data - -L J L, Day Observations Replicate number . 1 2 3 4 5 6 7 8 9 10 1 11 12 2 Renewal One Adult mortality (L = Uve, D = dead) L_ �-- ` I L- I`, L L E L I I-- L_ 5 Renewal Two Number of broods present Number of young produced Adult mortality (L=Live,D=dead) "t- Ila 1 1,-` IS t I', ` LL, 14 Ila L- IS -L J L, 7 Final Number of broods present Number of young produced Total young produced 1 114 10 l 1"L Zb \ IZ %1 1 1 p U 1 1 Z„ l 1 l�j Z`ti. 1 Z C is 1 1s L'1 1 ll Z'1 1 ) 1p l IL-, l� Z.9 p Z 1 3D Final adult mortality (L = Live, D = dead) X for 3rd Broods v of Male Adults (5 20%) Y'° Adults having 3rd Broods (>_ 80%) O� % Mortality (5 20%) �- Mean Offspring/Female (_> 15 offspring/surviving female) /� 26Z % CV (< 40%) If S?. SOP AT12 - Exhibit AT12.3, revision 11-01-14 I ��-----4 Page 1 of 1 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia (EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Test Bench Sheet Paired with Control # I Date: OJ -01,16 Client Earth Environmental Services NPDES # NCO020800 Facility Andrews WWTP outfall 001 Project # 1.010 County Cherokee Test Concentration (Chronic Limit) 13.0% Dilution preparation: Samples were not aerated or treated unless otherwise noted on this form. Control, dilution water and test renewal information are included on the Control Bench Sheet indicated above. t-rrerrncur rinuryses: Observations Initiation ml mL Total volume Sample Dilution water mL 39 261 300 t-rrerrncur rinuryses: Observations Initiation Renewal One Renewal Two 1 2 Initial Final Initial Final Initial Final Concentration Analyst 10 it /V6 Renewal One Mf `,r pH(S.U.) �v-7tr 7•fO 7,6 1 7 —f- 63 Test Dissolved oxygen (mg/L)7 1 7 -7.11 Concentration Conductivity (µmhos/cm) W4"y Z Z L �I *Temperature (°C) 'L�, '1$ Q Z} • 5 Z ,g 15 110 IS ` (� 1•j_ \'L 100%, Dissolved oxygen (mg/0 Conductivity (µmhos/cm) .7 Y a �J - i� Renewal Two Adult mortality (L = Live, D = dead) Sap —r r . J s• -{ ` *Total residual chlorine (mg/L) C 0.11 - .; 4 0.110 T K - Sample number Samples Ib p°10b ab Sample 2 J600\0if ( *Analyst identified for each day. performed DH- dissnived nxvnen —a ..... .:.a i the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual Chlorine Bench Sheet and transcribed to this bench sheet. Survival and Reproduction Data /narfnrmorf nt toct Day Observations Replicate number —4--S 2 Adult mortality 1 2 3 from control 11010 6 7 8 9 10 it 12 Renewal One (L=Live, o=dead) Number of broods present Z Z Z Z L ZLI1 Z 5 Numberofyoungproduced 110 IS ` (� 1•j_ \'L 1A 1S 13 is l(p Renewal Two Adult mortality (L = Live, D = dead) ` Number of broods present ( 1 1 i tLLt. LL Li Number of young produced mo I� l ,S 1-2- 16 1 S IS 1'Z. 1 �0 141 l 1 7 Total young produced Final 32 7A 1 V- , Z& 24 21 IA �r G Final adult mortality - (L=Uve,D=dead) L ET L � Test was initiated usint Samole 1. Samole 2 was used fnr R..—.k n— ram..11 �-a r...., u,., s[ �_ _ _ .. ___ ,.. , . ,. pnor to use with sort synthetic water • -----••--�- •- .— 1—wu�un.auun and warmed to 25.0 ± 1.0°C in a warm water bath. Test Results and Statistical Analyses: . Test results Statistics % Mortality • Mean offspring Q �V.2 per female 28 % Reduction from control 11010 t -Stat or Rank Sum t�r� •�m 1 -Tailed Critical 28 PASS or FAIL SOP AT12 - Exhibit AT12.2, revision 11-01-14 0011 Ceriodaphriia duhia Chronic Whole Effluent Toxicity Test ��; I EPA -821-R-02-013, Method 1000.0 - North Carolina Modification QR {{ Quality Control Verification of Data Entry, Calculations and Statistical Analyses Client: Andrews WWTP f Environmental Testing Solutions, Inc. Test dares: September 07 - 14, 2016 Project. number: 11720 Reveiwed by: \�a Concentration (%) Day Dumber of oproduced b replicate number Survival (%) Average. reproduction (offspring/female) v Coefficient of variation M Percent reduction from control (%) 1 2 3 4 5 6 7 8 9 10 11 12 Control 5 16 14 15 17 14 16 15 12 16 13 15 17 100 28.2 4.5 Not applicable 7 14 12 12 11 14 13 13 15 11 16 14 13 Total 30 26 27 28 28 29 28 27 27 29 29 1 30 13% 5 16 15 11 16 12 12 14 15 13 13 15 .16 100 28.2 6.2 0.0 7 16 14 15 12 16 15 15 12 16 14 11 14 Total 32 29 26 28 28 27T 29 27 29 27 26 30 Dunnett's MSD value: 1.564 MSD = Minimum Significant Difference PMSD: 5.6 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the,control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10°i percentile) = 13%. Upper PMSD bound determined by USEPA (90°i percentile) = 47%. Lower and upper PMSD bounds were determined from the I Oth and 90th percentile, respectively, of PMSD data from EPA's WET hnterlaboratory Variability Study (USEPA, 2001a; USEPA, 2001 b). USEPA. 2001a, 20016. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 -Appendix. EPA -821-13-01-004 and EPA -821-131-01-005. US Environmental Protection Agency; Cincinnati, 01-1. Andrvtrs 09-07-16 Ll roi 28.167 1.0000 28.167 26.000 30.000 4.499 12 13 28.167 1.0000 28.167 26.000 32.000 6.211 12 0.000 2.508 1.564 AuxitiaryTests r_-= ;, Statistic P.Y;Cr�t�cal- Skew Kurt Shapiro-Wiilk's Test indicates normal distribution (p > 0.01) 0.93258 0.884 0.54371 0.305 F -Test indicates equal variances (p = 0.30) 1.90566 5.31963 HomoscedasfictTest indicates nosignificant differences 1.56421 0.05553 0 2.33333 1 1,22 Treatments vs D -Control Andrews -09-07-16 z ap RE -z _ Environmental Testing Solutions, Inc. Ceriodaphnia_Surviv_al and Re_ production Tesf=Reproduction T_ - Start Date: 9/7/2016 Test ID: CdPFCRNC Sample ID: Andrew VWVTP End Date: 9/142016 Lab ID: ETS-Envir. Tesfing Sol. Sample Type: DMR -Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: n 10• D -Control 30.000 26.000 27.000 28.000 28.000 29.000 28.000 27.000 27.000 29.000 D -Control 29.000 30.000 13 32.000 29.000 26.000 28.0.00 28.000 27.000 29.000 27.000 29.000 27.000 13 26.000 30.000 roi 28.167 1.0000 28.167 26.000 30.000 4.499 12 13 28.167 1.0000 28.167 26.000 32.000 6.211 12 0.000 2.508 1.564 AuxitiaryTests r_-= ;, Statistic P.Y;Cr�t�cal- Skew Kurt Shapiro-Wiilk's Test indicates normal distribution (p > 0.01) 0.93258 0.884 0.54371 0.305 F -Test indicates equal variances (p = 0.30) 1.90566 5.31963 HomoscedasfictTest indicates nosignificant differences 1.56421 0.05553 0 2.33333 1 1,22 Treatments vs D -Control Andrews -09-07-16 Whole Effluent Toxid Chain -of -Custody Form 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee Andrews WWTP Purchase order: Species: Ceriodaphnia dubia Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP3B Sample information: (to be completed by sample collector). ' Composite sample: Sample location-L,A,11 L= vt- L/ 11ef Start date: S ( Time: C � � � Volume collected for testing: End date: Time: D, 0 t_ Number of containers filled for testing: 1 t Number of samples per hour: - 's, id J,__ � _ Method of transport to laboratory: Chilled during collection?��' Comments: If chilled, specify temperature: j G Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample- collector and facility personnel) Sample collected by: L-ZA Print Signature Dal. and time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: Ott Received at ETS by: FP14 --O_ W�-1/60 -DIG-110 0111, Prim Signature Date and time Print Custody seals intact?: Yes No Not used Samples received in good condition?: Yes No Tracking number: V�- Comments: o�g�mwu uaic into time Sample temperature upon receipt at ETS (°C): ySC. Total residual chlorine upon receipt at ETS: = = (DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Abscm Project number: 1(22it7 Sample number: 110 041010t0l0 WIN 4 �r 1 " ztl Environmental Testing Solutions, Inc. Whole Effluent Toxid Chain -of -Custody Form 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee Andrews WWTP Purchase order: Species: Ceriodaphnia dubia Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP3B Sample information: (to be completed by sample collector). ' Composite sample: Sample location-L,A,11 L= vt- L/ 11ef Start date: S ( Time: C � � � Volume collected for testing: End date: Time: D, 0 t_ Number of containers filled for testing: 1 t Number of samples per hour: - 's, id J,__ � _ Method of transport to laboratory: Chilled during collection?��' Comments: If chilled, specify temperature: j G Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample- collector and facility personnel) Sample collected by: L-ZA Print Signature Dal. and time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: Ott Received at ETS by: FP14 --O_ W�-1/60 -DIG-110 0111, Prim Signature Date and time Print Custody seals intact?: Yes No Not used Samples received in good condition?: Yes No Tracking number: V�- Comments: o�g�mwu uaic into time Sample temperature upon receipt at ETS (°C): ySC. Total residual chlorine upon receipt at ETS: = = (DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Abscm Project number: 1(22it7 Sample number: 110 041010t0l0 rte,tat l 09-0 1�, B ti I 351 Depot Street Print Relinquished by: Signature Asheville, NC 28801 Phone: (828) 350-9364 Print Relinquished by: sigaaturc Fax: (828) 350-9368 ;�e Environmental Testing solutions, Inc. Print Whole Effluent Toxicity Chain -of -Custody Form Date and time Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee Andrews WWTP Purchase order: Species: Ceriodaphnia dubia Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP3B Sample information: (to be completed by sample collector) Composite sample: Start date: (4o Time: End date: c! Time: Number of samples per hour: Chilled during collection? �( If chilled, specify temperature: Triple rinse sample container with sample before filling. Pack the sample container completely in ice. Imnle CIIStOdv[ (tn hr. ---1.4-A t... --1— .._n_ _ Sample location: t ;�tl;� '��'C;c r' 'e,, l/ c- 11� Volume collected for testing: t r -,r Number of containers filled for testing: 1 Method of transport to laboratory: S Comments: Completely fill the sample container with no air space. The sample must be < 6.0°C upon receipt at the laboratory. � .._...r ... .................... .rwur:y Ncia�uuctJ - Sample collected by: l 09-0 1�, / <Ac Print Relinquished by: Signature Date and time Print Relinquished by: sigaaturc Date and lime Print Signature Date and time aampte receipt mtormatlon: (to be completed by ETS personnel) Relinquished to ETS by: (1•{.711 1/1'. i C ld-e� j� ft�1 67 b Print signature Dam and it.. Custody seals intact?: ED Yes No Not used Samples received in good condition?: Yes No Tracking number: lj {+N Comments: Received by: Print Signature Date and time Received by: Print Siymatun: Dat. and time Received at ETS by: , gnam. Date and time Sample temperature upon receipt at ETS (°C): Total residual chlorine upon receipt at ETS: (DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Ab=t Project number:WTU Sample number: V140 b,c-A- 09-0 1�, , gnam. Date and time Sample temperature upon receipt at ETS (°C): Total residual chlorine upon receipt at ETS: (DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Ab=t Project number:WTU Sample number: V140 b,c-A- 1.14 1.12 1.10 1.0'8 1.06 1.04 1.02 2.5 2.0 z 1.5 in N U 1.0 0.5 1.4 1.3 1.2 1.1 1.0 0.9 0.8 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart USEPA Control Limits (± 2 Standard Deviations) USEPA Warning and Control Limits (75th and 90t1i Percentile CVs) Laboratory Warning and Control Limits (10th and 25th Percentile CVs) ............................................................................................... oa ��..p5 p5..o6 p9no1 ,p,..09 �A•.09 l? ,�o �b•%,� , ,1Z ,�> >y `Oti p9,pLA9 `p3 ��,oa �5 `oA �5 `oS ��,pb �� ,p1 ,10 �9 -%-10 :%6 Test date 0 7 -day IC,5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. — — Central Tendency (mean IC25) — — - Warning Limits (mean IC 25 t SA 10 or SMJ) ......... Control Limits (mean IC 2_ A25 f S , SA.90 , 1 or 2 Standard Deviations) mSumnN � "'N� n M, f s x'i S g _O Environmental Testing Solutions, Inc. 1.14 1.12 1.10 1.0'8 1.06 1.04 1.02 2.5 2.0 z 1.5 in N U 1.0 0.5 1.4 1.3 1.2 1.1 1.0 0.9 0.8 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart USEPA Control Limits (± 2 Standard Deviations) USEPA Warning and Control Limits (75th and 90t1i Percentile CVs) Laboratory Warning and Control Limits (10th and 25th Percentile CVs) ............................................................................................... oa ��..p5 p5..o6 p9no1 ,p,..09 �A•.09 l? ,�o �b•%,� , ,1Z ,�> >y `Oti p9,pLA9 `p3 ��,oa �5 `oA �5 `oS ��,pb �� ,p1 ,10 �9 -%-10 :%6 Test date 0 7 -day IC,5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. — — Central Tendency (mean IC25) — — - Warning Limits (mean IC 25 t SA 10 or SMJ) ......... Control Limits (mean IC 2_ A25 f S , SA.90 , 1 or 2 Standard Deviations) mSumnN � eM� € I t j } EnvironmentalTesting Solutions, Inc. January 03, 2017 Mr. Michael Ladd Earth Environmental Services 75 Bison Lane Murphy, NC 28906 RE: ETS PROJECT NUMBER: 11962 Dear Mr. Ladd: PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828)350-9368 Enclosed are toxicity test results for samples from the Andrews WWTP received by Environmental Testing Solutions, Inc. December 06 through December 08, 2016. Parameter Test Procedure EPA Method Final Code Number Result North Carolina Ceriodaphnia Chronic Effluent TGP3B Toxicity Procedure EPA -821-R-02-013 PASS (Ceriodaphnia Pass/Fail Toxicity Test) If this test was performed as an NPDES requirement or by Administrative Letter, please enter a P on the Effluent Discharge Monitoring Form (MR -1') for the collection date December 06, 2016 using the parameter code TGP3B. Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT -3) by January 31, 2017. If you have any questions concerning these results, please feel free to contact me. Sincerely, Jim umner Laboratory Director This report should not be reproduced, except in its entirety, without the written consent of Environmental Testing Solutions, Inc. The results in this report relate only to the samples submitted for analysis. North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600 South Carolina Certificate Number: Clean Water Act: 99053-001 ® t. ® Environmental Testing Solutions, Inc. Effluent Aquatic Toxicitv Report Form - Phase II Chronic Ceriodaphnia dubia PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828)350-9368 Date: January 03, 2017 Facility: Earth Environmental Services NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee Andrews WWTP Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments: Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor: Project: 11962 Samples: 161206.16, 161208.18 Mail Original To: North Carolina Department of Enviromnent and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Set -vice Center Start date: End date: Start time: End time: Raleigh, NC 27699-1621 12-07-16 12-14-16 1230 0700 Sample Information Collection start date: Grab: Composite duration: Alkalinity (mg/L CaCO3): Hardness (mg(L CaCO3): Conductivity (µmhos/cm): Total residual chlorine (mg/L): Sample Temp. at Receipt (°C): Sample 1 Sample 2 Control 12-05-16 12-07-16 R=.al2 13% 13% 13% 24-h 24-h Control 7.27 7.47 32, 31, 31 7.22 7.52 40, 40, 42 313 323 155, 158, 165 <0.10 <0.10 7.28 7.8 7.7 7.7 Test Information Treatment: Initial pH (SU): Final pH (SU): Initial DO (mg/L): Final DO (ing/L): Initial Temp. (°C): Final Temp. (°C): Organism Number Control OrEanisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan Stan Rcnc-1 l Rc=,,al2 Stan R-,,alI R=.al2 13% 13% 13% Control Control Control 7.27 7.47 7.41 7.22 7.52 7.54 7.53 7.41 7.30 7.47 7.42 7.28 7.8 7.7 7.7 7.8 7.8 7.9 7.9 7.8 7.8 7.9 7.8 7.8 24.8 24.8 24.7 24.8 24.6 24.7 25.1 24.8 25.1 24.9 25.1 25? Number of Young Produced 27 130 125 126 128 129 129 126 127 130 27 29 L Adult Survival: (L)ive, (D)ead L L L L L L L L L L L L c c.o Effluent Percentage13 % Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Number of Young Produced 128 129 26 28 25 29 31 32 28 28 32 32 29.0 Adult Survival: (L)ive, (D)ead L L L L L L L L L L L L1 -4.5 / Keductlon Effluent Percentage= reatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan lumber of Young Produced Ldult Survival: (L)ive, (D)ead %. Redncti, Effluent Percentage= -reatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan lumber of Young Produced tdult Survival: (L)ive, (D)ead %. KMW... Effluent Percentage= 'reatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan lumber of Young Produced Ault Survival: (L)ive, (D)ead % Rednciic Effluent Percentage= reatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mmn lumber of Young Produced .dolt Survival: (L)ive, (D)ead % Redaaic Overall Analysis: Result: PASS LOEC: >13% NOEC: 13% ChV: >13% DIf,'Q form AT -3 (8/91) Rev. 11195 Chronic Test Results Reproduction LOEC: Final Control Mortality (%): 0.0 %Control with 3rd Broods: 100 Control Reproduction CV: 6.0 48 Hour Mortality Method: Control: 0 of 12 IWC: 0 of 12 Significant?: No Final Mortality Significant at: No concentration Reproduction Analyses Reproduction LOEC: >13% Reproduction NOEC: 13% Overall Method: Homoscedastic t Normal Distribution: Yes Method: Shapiro-Wilk's Statistic: 0.952 Critical Value: 0.884 Equal Variances: Yes Method: F -Test Statistic: 1.983 Critical Value: 5.320 Non -Parametric Analysis (if applicable) Method: Effluent % Rank Sum Critical Sum 13% Page 1 of 1 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia (EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Control Bench Sheet Control #: �_ _ Date: 12-07-16 Test Groupinq Information. Test Organism Information: Facility Project # 6 < 24 -hours old 5 S tl�ib 4 1 F -SVI.N 11°t 3 %RIO 2 f%0t.A %0S t l �-- 1- Controlf._, 0.03 mL Daily Renewal Information: Organism Source: In-house Culture Age: < 24 -hours old Source (culture board): A Replicate # 1 2 3 4 5 6 1718 9 10 11 12 Culture board cup # L (. 1118 1 IS 11 1111151203 It, Date and time organisms were born between: -M ►'1-4-C) Ave'ra'ge transfer volume: 0.03 mL Transfer bowl information: pH (S.U.): Temperature (°C): '�,�•9 Chemical Analyses: Initiation Renewal One Initial Final Initial Final Concentration (s.U.) •'11.. i Renewal Two Initial Final Il. 1 WG Dissolved oxygen (mg/L) Test Initiation, renewal, feeding or 71 Selenastrum YWT Location Randomizing . Day Date termination SSW Batch (SV ,� �:s (COj - Control, SSW *Alkalinity (mg/L CaCO3) Adult mortality Batch Batch Incubator/Shelf Template Time Analyst 0 12-07-16 Initiation/Feeding IN cK r a h., It'1�•11a 1\.•t,�-ala 'j,-�Z ����.. (L = Live, D = dead) *Temperature (°C) Feed113a•i1,8 ,T=• -i. •� l.`1•� *� -i�-1 �'L5 • Z 1 12-08-16 ()!kty performed by the analysts indentiFed on the test bench sheets and transcribed to this bench sheet. '_- 2 12-09-16 Renewal JJFeeding 3D to r ro h ` +^ r + rt w'�* tsC t EMU it Z 114% 11 Number of broods present r n Feeding 3 12-10-16 Number of young produced I Z . 15 `b I`2.. IZ' 4 12-11-16 Feeding Q l Z k 1(n r t t r Adult mortality ps 1a e;} \,— i L L Renewal2/Feeding I-- k sYF r a 5 12-12-16 x.._ Feeding 1 6 12-13-16 1 I 1 I 1 I LIL 12-14-16 Termination U- L 7 Chemical Analyses: Initiation Renewal One Initial Final Initial Final Concentration (s.U.) •'11.. i Renewal Two Initial Final Il. 1 WG Control Acceptance Criteria: % of Male Adults (<_ 20%) Dissolved oxygen (mg/L) -I A 71 fle 't-o_ 4 /IV I 6 Conductivity (µmhos/cm) l55 .,tea ;y (SV ,� �:s (COj Day Control, SSW *Alkalinity (mg/L CaCO3) Adult mortality aku ��, t Fra: 3 �E3ac *Hardness ( g m /LCaCO3) IN cK r a h., 5 2 (L = Live, D = dead) *Temperature (°C) �}`. �' ,T=• -i. •� l.`1•� *� -i�-1 �'L5 • Z -Analyst identified for each day, performed pH, dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table. Alkalinity and hardness performed by the analysts indentiFed on the test bench sheets and transcribed to this bench sheet. Control Acceptance Criteria: % of Male Adults (<_ 20%) Observations Replicate number 1 2 1 3 4 5 6 7 8 9 30 11• 12 Day 2 Adult mortality L L Renewal One (L = Live, D = dead) '_- 2 Z` Z Number of broods present 5 Number of young produced I Z . 15 `b I`2.. IZ' 1 l 10 1� l Z I 1(n 1 V` Renewal Two Adult mortality X_ \,— L L tv„ I-- "— (L = Live, D =dead) 1 t 1 1 I 1 I 1 I LIL Number of broods present L �5 IS 12- l J l �j 13 15 11 1 i 5q Number of young produced 7 Total young produced �.� ,LS Zb Ze, ZC` Zto wi -n Z Final Final adult mortality L t✓ L (L= Live, D = dead) X for 3rd Broods Control Acceptance Criteria: % of Male Adults (<_ 20%) M. %Adults having 3rd Broods (>_ 80%) too. • Mortality (<_ 20%) Mean offspring/Female (-e 15 offspring/surviving female) L t 8 CV (< 40%) (p,07. SOP AT12 - Exhibit AT12.3, revision 11-01-14 �-v Page 1 of 1 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia (EPA -821-R-02-013 Method 1002.0, NC Modification - December 2010, Version 3.0) - Test Bench Sheet Paired with Control # I Date: I—L, TI'lik, Client Earth Environmental Services NPDES # NCO020800 Facility Andrews WWTP outfall 001. Project # I County Test Concentration (Chronic Limit) 13.0% Dilution preparation: Samples were not aerated or treated unless otherwise noted on this form. Control, dilution water and test -renewal information are included on the Control Bench Sheet indicated above. Cherokee mL Sample mL Dilution water Totalvolume mL 39 261 300 Chemical Analyses: Initiation Initial Final Renewal One Renewal Two Initial Final Initial Final Concentration Analyst HS 4/6 46 t-- 0%-/ NG Test Concentration PH (S.U.) —4•11 7,j3 7. Y7 /1-41 a.4i 7.30 Dissolved oxygen (mg/L) 7.8 '71 7.7 7 Conductivity (µmhos/cm) /gp X i' 1 a *Temperature (°C) �� •S -A. 8 100% pH (S. U.) 8 y y k ; :7.2 .�,� ,� � �H . �. %. SD 4x �s WOW Dissolved oxygen (mg/L) 7.1 7 •� Conductivity (µmhos/cm) 3/3 *Total residual chlorine (mg/L) 4 p .l, D Spa 10 a 3,! U Sample number Sample 1 Il I-Lo1o.16 sample 2 16li-LO. I$ -r muysr taennnea for eacn aay, pertormea prt, atssotvea oxygen ana conauctivtty measurements omy. i emperamres pertormea at ttie time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual Chlorine Bench Sheet and transcribed to this bench sheet. Survival and Reproduction Data (performed at test concentration) : Day Observations Replicate number 1 2 3 . 4 5 6 7 8 9 10 it 12 2 Adult mortality Renewal One (L = Live, D = dead) Number of broods present 2 •L 5 Number of young produced IS I� 13 �� 1- IS IS L -L IS 1-1 l RenewalTwo Adult mortality (L = Live, D = dead) Number of broods present %L I LL 1 t 1 1 L 1 Number of young produced 1 I^2..{�j loFinal Total young produced Z% -46Z-O t—V-532EI ZA 3Z It —L& 3,Z37- Final adult mortality (L= Live, D = dead) , cx was 1111 ua I.— usmg 3,11 [1 a. -m npre < was Us ror nenewals une tuay [J ana iwo tuay �). dam pies were iniurea to the test concentration prior to use witn sott synthetic water and warmed to 25.0 ± 1.0°C in a warm water bath. Comments: Test Results and Statistical Analyses: Test results Statistics %Mortality 1 •5�2 Mean offspring 2R.o per female z• % Reduction "�•�7 from control ILi t -Stat or Rank Sum 1 •5�2 1-Tailed�j� z• Critical -M/ PASS or FAIL PASS SOP AT12 - Exhibit AT12.2, revision 11-01-14 - �.. r t YS ,-7 f ...P .i ;i Environmental Testing Solutions, Inc. Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA -821-R-02-013, Method 1000.0 - North Carolina Modification Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: Test dates: Andrews WWTP December 07- 14, 2016 Project number: 11962 Reveiwed by: N.. Concentration (%) Day Number of o ng produced by re licate number Survival ('%,) Average reproduction (offspring/female) Coefficient of variation (%.) Percent reduction from control (1/4) 1 2 3 4 5 6 7 8 9 10 11 12 Control 5 12 15 13 12 12 14 16 13 12 13 16 14 1 100 27.8 6.0 Not applicable 7 15 15 12 14 16 15 13 13 15 17 11 15 Total 27 30 25 26 28 29 29 26 27 30 27 29 13% 5 14 15 14 13 13 17 15 15 12 15 17 18 100 29.0 8.1 -4.5 7 14 14 12 15 12 12 16 17 16 13 15 14 Total 28 29 26 28 25 29 31 32 28 28 32 32 Dunnett's A1SD value: 2.074 MSD = MinitnU n Significant Difference PIVISD: 7.5 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound detennined by USEPA (10`x' percentile) = 13%. Upper PMSD bound determined by USEPA (90`x' percentile) = 47%. Lower and upper PMSD bounds were detennined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001 b). USEPA. 2001 a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short -tens Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2 -Appendix. EPA -821-B-01-004 and EPA -821-13-01-005. US Environmental Protection Agency, Cincinnati, OH. Andrews 12-07-16 4 1 0 JO i TrarisformrUntransformed :'-Critical >, 1 Tailed Cone% Mean _N Mean Mean; Min ..'Max .: CU%_ r D -Control 27.750 1.0000 27.750 25.000 30.000 5.976 12 1.98347 13 29.000 1.0450 29.000 25.000 32.000 8.053 12 'v MSDu:- MSDp., ".MSB" „-=E ,- df - HomoscedastictTestindicatesnosignificantdifferences 2.07405 0.07474 9.375 4.10227 0.14484 1,22 Treatments vs D -Control ` Environmental Testing Solutions, Inc. -CeriodaphniaSurvivaland:ReproductionTest-Reproduction-_ , :, ='•,' j Start Date: 12/7/2016 Test ID: CdPFCRNC Sample ID: Andrews VWVfP End Date: 12/14/2016 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR -Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Cone"/° 1 .Z, ;: 3;' 4:' ..- `5 6 7 ... _.$-_ ``9 10 i D -Control 27.000 30.000 25.000 26.000 28.000 29.000 29.000 26.000 27.000 30.000 D -Control 27.000 29.000 13 28.000 29.000 26.000 28.000 25.000 29.000 31.000 32.000 28.000 28.000 13 32.000 32.000 i TrarisformrUntransformed :'-Critical >, 1 Tailed Cone% Mean _N Mean Mean; Min ..'Max .: CU%_ t Stat .Critical 'MSD D -Control 27.750 1.0000 27.750 25.000 30.000 5.976 12 1.98347 13 29.000 1.0450 29.000 25.000 32.000 8.053 12 -1.512 2.508 2.074 ?Auxiliary Tests,'• , -, Statistic' :'-Critical -;Skew', Kurt = Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.952 0.884 -0.0618 -0.7378 F -Test indicates equal variances (p = 0.27) 1.98347 5.31963 ,Ii ypothesisiTest,(1 ,tail; 0'.01) , '.• MSDu:- MSDp., ".MSB" „-=E ,- df - HomoscedastictTestindicatesnosignificantdifferences 2.07405 0.07474 9.375 4.10227 0.14484 1,22 Treatments vs D -Control Andreins 12-07-16 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Whole Effluent Toxicity Chain -of -Custody Form Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: 'Cherokee Andrews WWTP Purchase order: Species: Ceriodapitnia dubia Effluent dilution: 13% - Test type: Pass/Fail Chronic Parameter code: TGP3B Sample information: (to be completed by sample collector) Composite sample: r Sample location: -till 1-WT—1e11 ao� Start date: Time: 0� Volume collected for testing: t! End date: Time: "QC Number of containers filled for testing: j Number of samples per hour: l,�J. Method of transport to laboratory: ES Chilled during collection? Y e s Comments: If chilled, specify temperature: Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0aC upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) S/aim/pplee collected by: gli 13 7Z� 73Print Dale and time Signature Relinquished by: Received by: Environmental Testing Solutions, Inc. 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Whole Effluent Toxicity Chain -of -Custody Form Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: 'Cherokee Andrews WWTP Purchase order: Species: Ceriodapitnia dubia Effluent dilution: 13% - Test type: Pass/Fail Chronic Parameter code: TGP3B Sample information: (to be completed by sample collector) Composite sample: r Sample location: -till 1-WT—1e11 ao� Start date: Time: 0� Volume collected for testing: t! End date: Time: "QC Number of containers filled for testing: j Number of samples per hour: l,�J. Method of transport to laboratory: ES Chilled during collection? Y e s Comments: If chilled, specify temperature: Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0aC upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) S/aim/pplee collected by: 13 73Print Dale and time Signature Relinquished by: Received by: Print Signature Dale and lime Print Signature Dat. and lime Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: 1 Print Signature Date and time Custody seals intact?: = Yes No Not used Samples received in good condition?: Yes No Tracking number: (J PC Comments: Receivel:tt ETS bv: rant mgnature uatc ana time Sample temperature upon receipt at ETS (nC): Total residual chlorine upon receipt at ETS: = (DPD Presence/Absence Indicator, MDL = 0.10 mg/L) Present Absent Project number: I IOltO2 Sample number: 1 �AZC a,L(,0 13 rant mgnature uatc ana time Sample temperature upon receipt at ETS (nC): Total residual chlorine upon receipt at ETS: = (DPD Presence/Absence Indicator, MDL = 0.10 mg/L) Present Absent Project number: I IOltO2 Sample number: 1 �AZC a,L(,0 ,sP � 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828)350-9368 Environmental Testing Solutions, Inc. Whole Effluent Toxicity Chain -of -Custody Form Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee Andrews WWTP Purchase order: Species: Cer[odaphnia dub[a Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP3B Sample information: (to be completed by sample collector) Composite sample: Start date: L �� (% End date: l �V Number of samples per hour: Chilled during collection? If chilled, specify temperature: Sample location t O & f F-r—K'i ee' tJ�C►l/� i /1' -�e� esting: 1 e Time: 3:��V Volume collected for testing: Time: Number of containers filled for t L S."' 1 $R ia,( Method of transport to laboratory \j Comments: Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: Print Signature Date and lime Relinquished by: Print Signature Dale and time Relinquished by: Print Signature Dale and lima Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: 'aP� ffi ,.�_ Print Signature Date and lime Custody seals intact?: = O Ycs No Not used Samples received in good condition?: = Yes No Tracking number: Comments: �44JP Received by: Print Signature Dale and time Received by: Print Signature Dalc and time Received at ETS by: Print Signature Date and lime Sample temperature upon receipt at ETS (°C): �r5 Total residual chlorine upon receipt at ETS: = Ei (DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Absent pl $ Project number: Sample number: J' V IM 119 Quad: Andrews, N.C. N 0 0 0 2 0 8 0 0 Subbasin:040502 Town of Andrews WWTP Latitude: 35°11'51" Longitude: 83°50'46" Receiving Stream: Valle} River Stream Class: C -Trout HUC#: 06020002 Facility F. �► r Location ,1 SCALE 1:24000 IN' E'IEV. 1711W 'tl ti•" �,.' ` .. C)P' `k' E. IM"T EYEV, 1707.49 '" �tbo '_fi GRAVITY Q—.±cj f1 ti �r'�,3,: •\ \ \. F\ItiTIhG. LUD.,xt: DE?YINC. LTFRt _ _ i� AERtTM. ,:� � .1.UQt k: •:ti Ct:NC r i4, .,\ �s TlNc ! l WD LAS 1, \•` r 1 �`nC . EwsTlNuss i E�SIF,iSfi:t C - EXISTI BUDGE DRYIW, BLDS 1 k f s .. . l xISti`IC VANHOLE -TOP El— 1716:9• : 1 1 'Al DIP r INV E1, t7t369; -_ 8" lv,µ. - TC ILL 1714.14 i c -j k EL 1712.93 10 ,` ! i^• �. t r :; % " \ ``, y,.. 1: G V." I E!�ISTiki• f =Vo� ICS LLL JI _ ll HFd `WORKS ANEiOY VAi 4i ; i J't% y EXISTING ErISTING c+ ADONED v I . IIECKE STRUCTUREPWn .71 D 142 De t .t. '•. ! �a A.," i i "`idllli'E< ire ! l \.� � ( .. j°k 3"4C.lirrTC�x VJ r11U P4'C;rGRTY 5�15ER :� EL 171266' t� PO J _ .' . AS�r�i PARS �" �Y... 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