HomeMy WebLinkAboutNC0020800_Renewal (Application)_20170321Water Resources
ENVIRONMENTAL QUALITY
March 21, 2017
Ms. Nancy Curtis, Mayor
Town of Andrews
PO Box 1210
Andrews, NC 28901
Subject: Renewal Application
Application No. NCO020800
Andrews WWTP
Cherokee County
Dear Ms. Curtis:
ROY COOPER
Governor
MICHAEL S. REGAN
Secretary
S. JAY ZIMMERMAN
Director
. The Water Quality Permitting Section acknowledges receipt of your permit application and
supporting documentation received on March 20, 2017. The primary reviewer for this renewal
application is Julie Grzyb.
The primary reviewer will review your application, and she will contact you if additional
information is required to complete your permit renewal. Per G.S. 150B-3 your current permit
does not expire until permit decision on the application is made. Continuation of the current permit
is contingent on timely and sufficient application for renewal of the current permit.
Please respond in a timely manner to requests for additional information necessary to
complete the permit application. If you have any additional questions concerning renewal of the
subject permit, please contact Julie Grzyb at 919-807-6389 or Julie.Grzyb@ncdenr.gov.
cc: Central Files
NPDES-
Asheville Regional Office
Sincerely,
? tiea %&*vd
Wren Thedford
Wastewater Branch
State of North Carolina I Environmental Quality I Water Resources
1617 Mail Service Center I Raleigh, North Carolina 27699-1617
919-807-6300
3/15/17
NPDES PERMIT RENEWAL APPLICATION FOR ANDREWS, NC WASTEWATER TREATMENT PLANT
Permit #: NCO020800
Facility Address:
464 Reagan Ave.
Andrews, NC. 28901
Included:
• NPDES Permit Renewal Application
Attached Documentation:
RECEIVEDINCDEQUYR
MAR 2 0 2017
P �terQuality
ttiOg Section
• Pollutant Scan Analysis Data for Part B, Section 13.6 (3 sample analysis from 2/13, 5/14, and
2/15)
• Pollutant Scan Analysis Data for Part D (3 sample analysis from 2/13, 5/14, and 2/15)
• Complete Toxicity Test Results for 2016 for Part E, Section E
• Topographic Map
• Basic Process Flow Diagram
FlACILITY'NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
ANDREWS WWTP, NCO020800
RENEWAL
HIWASSEE
FORM
'
2A
N"P'DES FORM 2A �4PPLICATION ®`UER\/LEVII ' �"
NPDES
APPLICATION OVERVIEW
Form 2A has been developed in a modular format and consists of a "Basic Application Information" packet
and a "Supplemental Application Information" packet. The Basic Application Information packet is divided
into two parts. All applicants must complete Parts A and C. Applicants with a design flow greater than or
equal to 0.1 mgd must also complete Part B. Some applicants must also complete the Supplemental
Application Information packet. The following items explain which parts of Form 2A you must complete.
BASIC APPLICATION INFORMATION:
A. Basic Application Information for all Applicants. All applicants must complete questions A.1 through A.8. A treatment works
that discharges effluent to surface waters of the United States must also answer questions A.9 through A.12.
B. Additional Application Information for Applicants with a Design Flow >_ 0.1 mgd. All treatment works that have design flows
greater than or equal to 0.1 million gallons per day must complete questions B.1 through 13.6.
C. Certification. All applicants must complete Part C (Certification).
SUPPLEMENTAL APPLICATION INFORMATION:
D. Expanded Effluent Testing Data. A treatment works that discharges effluent to surface waters of the United States and meets
one or more of the following criteria must complete Part D (Expanded Effluent Testing Data):
1. Has a design flow rate greater than or equal to 1 mgd,
2. Is required to have a pretreatment program (or has one in place), or
3. Is otherwise required by the permitting authority to provide the information.
E. Toxicity Testing Data. A treatment works that meets one or more of the following criteria must complete Part E (Toxicity Testing
Data):
1. Has a design flow rate greater than or equal to 1 mgd,
2. Is required to have a pretreatment program (or has one in place), or
3. Is otherwise required by the permitting authority to submit results of toxicity testing.
F. Industrial User Discharges and RCRA/CERCLA Wastes. A treatment works that accepts process wastewater from any
significant industrial users (SIUs) or receives RCRA or CERCLA wastes must complete Part F (Industrial User Discharges
and RCRA/CERCLA Wastes). SIUs are defined as:
1. All industrial users subject to Categorical Pretreatment Standards under 40 Code of Federal Regulations (CFR) 403.6 and
40 CFR Chapter I, Subchapter N (see instructions); and
2. Any other industrial user that:
a. Discharges an average of 25,000 gallons per day or more of process wastewater to the treatment works (with certain
exclusions); or
b. Contributes a process wastestream that makes up 5 percent or more of the average dry weather hydraulic or organic
capacity of the treatment plant; or
C. Is designated as an SIU by the control authority.
G. Combined Sewer Systems. A treatment works that has a combined sewer system must complete Part G (Combined Sewer
Systems).
Y
ALL APPLICANTS`'MUST::COMPLETE;"PART'-C (CERTIFICATION)
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Pagel of 22
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
ANDREWS WWTP, NCO020800
RENEWAL
HIWASSEE
BASIC,APPLICATION INFORMATION
PART A. BASIC APPLICATION INFORMATION FORALL APPLICANTS:
All treatment works must complete questions A.1 through A.8 of this Basic Application Information Packet.
A.I. Facility Information.
Facility Name ANDREWS WASTEWATER TREATMENT PLANT
Mailing Address PO BOX 1210
ANDREWS, NC 28901
Contact Person NANCY CURTIS TIM WOOD
Title MAYOR WWTP ORC
Telephone Number (828) 321-3113 (828) 321-3006
Facility Address 464 REAGAN AVE.
(not P.O. Box) ANDREWS NC 28901
A.2. Applicant Information. If the applicant is different from the above, provide the following:
Applicant Name TOWN OF ANDREWS
Mailing Address PO BOX 1210
ANDREWS NC 28901
Contact Person NANCY CURTIS
Title MAYOR
Telephone Number (828) 321-3113
Is the applicant the owner or operator (or both) of the treatment works?
® owner ❑ operator
Indicate whether correspondence regarding this permit should be directed to the facility or the applicant.
❑ facility ® applicant
A.3. Existing Environmental Permits. Provide the permit number of any existing environmental permits that have been issued to the treatment works
(include state -issued permits).
NPDES NCO020800 PSD
UIC Other
RCRA Other
A.4. Collection System Information. Provide information on municipalities and areas served by the facility. Provide the name and population of each
entity and, if known, provide information on the type of collection system (combined vs. separate) and its ownership (municipal, private, etc.).
Name Population Served Type of Collection System Ownership
Town of Andrews 1500 Separate Municipal
Total population served
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 2 of 22
FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE
A.S. Indian Country.
a. Is the treatment works located in Indian Country?
❑ Yes ® No
b. Does the treatment works discharge to a receiving water that is either in Indian Country or that is upstream from (and eventually flows
through) Indian Country?
❑ Yes ® No
A.S. Flow. Indicate the design flow rate of the treatment plant (i.e., the wastewater flow rate that the plant was built to handle). Also provide the
average daily flow rate and maximum daily flow rate for each of the last three years. Each year's data must be based on a 12 -month time period
with the 12th month of "this year' occurring no more than three months prior to this application submittal.
a. Design flow rate 1.5 mgd
Two Years Ago Last Year This Year
b. Annual average daily flow rate 0.616 (2014) 0.713 (2015) 0.570 (2016)
C. Maximum daily flow rate 1.850 (10/15/14) 2.143 (12/31/15) 1.961 (2/25/16)
A.7. Collection System. Indicate the type(s) of collection system(s) used by the treatment plant. Check all that apply. Also estimate the percent
contribution (by miles) of each.
® Separate sanitary sewer 100 %
❑ Combined storm and sanitary sewer %
A.8. Discharges and Other Disposal Methods.
a. Does the treatment works discharge effluent to waters of the U.S.? ® Yes ❑ No
If yes, list how many of each of the following types of discharge points the treatment works uses:
I. Discharges of treated effluent 1
ii. Discharges of untreated or partially treated effluent None
iii. Combined sewer overflow points N/A
iv. Constructed emergency overflows (prior to the headworks) NIA
V. Other I NIA
b. Does the treatment works discharge effluent to basins, ponds, or other surface impoundments
that do not have outlets for discharge to waters of the U.S.? ❑ Yes ® No
If yes, provide the following for each surface impoundment:
Location:
k Annual average daily volume discharge to surface impoundment(s)
Is discharge ❑ continuous or ❑ intermittent?
C. Does the treatment works land -apply treated wastewater?
If yes, provide the following for each land application site:
Location:
Number of acres:
Annual average daily volume applied to site:
Is land application ❑ continuous or ❑ intermittent?
d. Does the treatment works discharge or transport treated or untreated wastewater to another
treatment works? 1.
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.
r
mgd
❑ Yes ® No
mgd
❑ Yes ® No
Page 3 of 22
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
ANDREWS WWTP, NCO020800
RENEWAL
HIWASSEE
If yes, describe the mean(s) by which the wastewater from the treatment works is discharged or transported to the other treatment works
(e.g., tank truck, pipe).
If transport is by a party other than the applicant, provide:
Transporter Name
Mailing Address
Contact Person
Title
Telephone Number ( 1
For each treatment works that receives this discharge, provide the following:
Name
Mailing Address
Contact Person
Title
Telephone Number ( 1
If known, provide the NPDES permit number of the treatment works that receives this discharge
Provide the average daily flow rate from the treatment works into the receiving facility.
mgd
e. Does the treatment works discharge or dispose of its wastewater in a manner not included
in A.8. through A.8.d above (e.g., underground percolation, well injection): ❑ Yes
® No
If yes, provide the following for each disposal method:
Description of method (including location and size of site(s) if applicable):
Annual daily volume disposed by this method:
Is disposal through this method ❑ continuous or ❑ intermittent?
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 4 of 22
n
FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NCO020800 RENEWAL HIWASSEE
WASTEWATER DISCHARGES:
If you answered "Yes" to question A.8.a, complete questions A.9 through A.12 once for each outfall (including bypass points) through
which effluent is discharged. Do not include information on combined sewer overflows in this section. If you answered "No" to question
A.8.a, go to Part B, "Additional Application Information for Applicants with a Design Flow Greater than or Equal to 0.1 mgd."
A.9. Description of Outfall.
a. Outfall number 001
b. Location VALLEY RIVER, ANDREWS, NC. 28901
(City or town, if applicable) (Zip Code)
(County)
35°11'51'
(Latitude)
C. Distance from shore (if applicable)
d. Depth below surface (if applicable)
e. Average daily flow rate
f. Does this outfall have either an intermittent or a periodic discharge?
If yes, provide the following information:
Number f times per year discharge occurs:
Average duration of each discharge:
Average flow per discharge:
Months in which discharge occurs:
g. Is outfall equipped with a diffuser?
(State)
(Longitude)
N/A ft.
N/A ft.
0.633 mgd
❑ Yes ® No (go to A.9.g.)
❑ Yes ® No
mgd
A.10. Description of Receiving Waters.
a. Name of receiving water VALLEY RIVER
b. Name of watershed (if known) HIWASSEE RIVER WATERSHED
United States Soil Conservation Service 14 -digit watershed code (if known):
C. Name of State Management/River Basin (if known):
United States Geological Survey 8 -digit hydrologic cataloging unit code (if known):
d. Critical low flow of receiving stream (if applicable)
acute cfs chronic cfs
e. Total hardness of receiving stream at critical low flow (if applicable): mg/I of CaCO3
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.
Page 5 of 22
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
ANDREWS WWTP, NCO020800
RENEWAL
HIWASSEE
A.11. Description of Treatment
a. What level of treatment are provided? Check all that apply.
E Primary ® Secondary
❑ Advanced ❑ Other. Describe:
b. Indicate the following removal rates (as applicable):
Design BOD5 removal or Design CBOD5 removal 85 %
Design SS removal 85 %
Design P removal %
Design N removal %
Other %
C. What type of disinfection is used for the effluent from this outfall? If disinfection varies by season, please describe:
CHLORINATION
If disinfection is by chlorination is dechlorination used for this outfall? E Yes, ❑ No
Does the treatment plant have post aeration? ❑ Yes E No
A.12. Effluent Testing Information. All Applicants that discharge to waters of the US must provide effluent testing data for the following
parameters. Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is
discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data
collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of
40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a
minimum, effluent testing data must be based on at least three samples and must be no more than four and one-half years apart.
Outfall number: 001
MAXIMUM DAILY VALUE -
AVERAGE DAILY VALUE
PARAMETER
Value
Units
Value
Units Number of Samples
pH (Minimum)
5.8
S.U.
pH (Maximum)
7.4
s.u.
Flow Rate
2.685
MGD
0.684
MGD 1460
Temperature (Winter)
16.1
°C
11.35
°C 92
Temperature (Summer)
25.1
°C
21.5
°C 92
* For pH please report a minimum and a maximum daily value
MAXIMUMDAILY'``
AVERAGE DAILY''DISCHARGE
DISCHARGE
ANALYTICAL
POLLUTANT,
METHOD
ML/MDL
Conc.
Units
Conc.
Units
Number of
Samples
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
BIOCHEMICAL OXYGEN
BOD5
74.8
MG/L
23.3
MG/L
1460
SM5210B
2.0
DEMAND (Report one)
CBOD5
FECAL COLIFORM
>9000
#/100ML
62
#/100ML
1460
SM9222D
1/100m1
TOTAL SUSPENDED SOLIDS (TSS)
95.6
MG/L
1 19.7
MG/L
1460
SM2540D
5.0
END OF' PART A.
REFER TO, THE APPLICATION! OVERVIEW_(PAGE'I) TO DETERMINE'WHICH OTHER PARTS
OF FORM.2A YOU ,MUST COMPLETE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 6 of 22
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
ANDREWS WWTP, NCO020800
RENEWAL
HIWASSEE
BASIC APPLICATION INFORMATION. -
PART:B.- -ADDITIONAL APPLICATION INFORMATION FOR APPLICANTlq,WITH°'A DESIGN FLOW GREATER THAN OR.
EQUAL- TO;0.1 MOD (100;000 gallons;per day). ^,
All applicants with a design flow rate 2 0.1 mgd must answer questions B.1 through B.6. All others go to Part C (Certification).
B.1. Inflow and Infiltration. Estimate the average number of gallons per day that flow into the treatment works from inflow and/or infiltration.
100,000 gpd
Briefly explain any steps underway or planned to minimize inflow and infiltration.
Ongoing identification and repair of problem areas by Sewer Collection Dept.
B.2. Topographic Map. Attach to this application a topographic map of the area extending at least one mile beyond facility property boundaries. This
map must show the outline of the facility and the following information. (You may submit more than one map if one map does not show the entire
area.)
a. The area surrounding the treatment plant, including all unit processes.
b. The major pipes or other structures through which wastewater enters the treatment works and the pipes or other structures through which
treated wastewater is discharged from the treatment plant. Include outfalls from bypass piping, if applicable.
c. Each well where wastewater from the treatment plant is injected underground.
d. Wells, springs, other surface water bodies, and drinking water wells that are: 1) within ''% mile of the property boundaries of the treatment
works, and 2) listed in public record or otherwise known to the applicant.
e. Any areas where the sewage sludge produced by the treatment works is stored, treated, or disposed.
f. If the treatment works receives waste that is classified as hazardous under the Resource Conservation and Recovery Act (RCRA) by truck, rail,
or special pipe, show on the map where the hazardous waste enters the treatment works and where it is treated, stored, and/or disposed.
B.3. Process Flow Diagram or Schematic. Provide a diagram showing the processes of the treatment plant, including all bypass piping and all
backup power sources or redunancy in the system. Also provide a water balance showing all treatment units, including disinfection (e.g.,
chlorination and dechlorination). The water balance must show daily average flow rates at influent and discharge points and approximate daily flow
rates between treatment units. Include a brief narrative description of the diagram.
B.4. Operation/Maintenance Performed by Contractor(s).
Are any operational or maintenance aspects (related to wastewater treatment and effluent quality) of the treatment works the responsibility of a
contractor? ® Yes ❑ No
If yes, list the name, address, telephone number, and status of each contractor and describe the contractor's responsibilities (attach additional
pages if necessary).
Name: Earth Environmental Services
Mailing Address: 75 Bison Ln.
Murphy, NC. 28906
Telephone Number: (828) 837-9543
Responsibilities of Contractor: Laboratory Analysis/Sampling
B.S. Scheduled improvements and Schedules of Implementation. Provide information on any uncompleted implementation schedule or
uncompleted plans for improvements that will affect the wastewater treatment, effluent quality, or design capacity of the treatment works. If the
treatment works has several different implementation schedules or is planning several improvements, submit separate responses to question B.5
for each. (If none, go to question B.6.)
a. List the outfall number (assigned in question A.9) for each outfall that is covered by this implementation schedule.
001
b. Indicate whether the planned improvements or implementation schedule are required by local, State, or Federal agencies.
❑ Yes ® No
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 7 of 22
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
ANDREWS WWTP, NCO020800
RENEWAL
HIWASSEE
C. If the answer to B.5.b is "Yes," briefly describe, including new maximum daily inflow rate (if applicable).
N/A
d. Provide dates imposed by any compliance schedule or any actual dates of completion for the implementation steps listed below, as
applicable. For improvements planned independently of local, State, or Federal agencies, indicate planned or actual completion dates, as
applicable. Indicate dates as accurately as possible.
Schedule Actual Completion
Implementation Stage MM/DD/YYYY MM/DD/YYYY
- Begin Construction 04/30/2017 (Tentative Date)
- End Construction 12/31/2017 (Tentative Date)
- Begin Discharge
- Attain Operational Level
e. Have appropriate permits/clearances concerning other Federal/State requirements been obtained? ® Yes ❑ No
Describe briefly:
B.6. EFFLUENT TESTING DATA (GREATER THAN 0.1 MGD ONLY).
Applicants that discharge to waters of the US must provide effluent testing data for the following parameters. Provide the indicated
effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include information
on combine sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate
QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a minimum effluent testing data must be
based on at least three pollutant scans and must be no more than four and on -half years old.
Outfall Number: 001
MAXIMUM DAILY
AVERAGE DAILY DISCHARGE
DISCHARGE
ANALYTICAL
POLLUTANT
METHOD
MLJMDL
Conc.,
Units.
Conc.
Units
Number of
Samples
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
AMMONIA (as N)
CHLORINE (TOTAL
RESIDUAL, TRC)
DISSOLVED OXYGEN
TOTAL KJELDAHL
NITROGEN (TKN)
NITRATE PLUS NITRITE
NITROGEN
OIL and GREASE
PHOSPHORUS (Total)
TOTAL DISSOLVED SOLIDS
(TDS)
OTHER
ENO -OF PART B.
REFER'TO THE AP.PLICATION,OVERVIEW:(PAGE 1).TO'DETERMINE WLHICH OTHER;PARTS
.OF'FORM 2A YOU VUST COMPLETE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 8 of 22
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
ANDREWS WWTP, NCO020800
RENEWAL
HIWASSEE
'BASIC' APP:LICAT1.0 INFORMATION r
PART•C. C-ERTIFICATION!,:'
All applicants must complete the Certification Section. Refer to instructions to determine who is an officer for the purposes of this
certification. All applicants must complete all applicable sections of Form 2A, as explained in the Application Overview. Indicate below which
parts of Form 2A you have completed and are submitting. By signing this certification statement, applicants confirm that they have reviewed
Form 2A and have completed all sections that apply to the facility for which this application is submitted.
Indicate which parts of Form 2A you have completed and are submitting:
® Basic Application Information packet Supplemental Application Information packet:
® Part D (Expanded Effluent Testing Data)
® Part E (Toxicity Testing: Biomonitoring Data)
® Part F (Industrial User Discharges and RCRA/CERCLA Wastes)
❑ Part G (Combined Sewer Systems)
•,.
ALL AP.;PLICANTS MUST-COMPLETE„TWE•FOL'LOWING CERTIF.ICATION.,
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system
designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who
manage the system or those persons directly responsible for gathering the information, the information is, to the best of my knowledge and belief, true,
accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment
for knowing violations.
Name and official title Ma or Nancy Curtis
Signature
Telephone number (828) 321-3113
Date signed
Upon request of the permitting authority, you must submit any other information necessary to assure wastewater treatment practices at the treatment
works or identify appropriate permitting requirements.
SEND COMPLETED FORMS TO:
NCDENR/ DWR
Attn: NPDES Unit
1617 Mail Service Center
Raleigh, North Carolina 27699-1617
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 9 of 22
FACILITY NAME AND PERMIT NUMBER:
ANDREWS WWTP, NCO020800
PERMIT ACTION REQUESTED:
RENEWAL
RIVER BASIN:
HIWASSEE
SUPPLEMENTAL APPLICATION, INFORMATION
PART D. fXPANDED: EFFLUENT TESTING DATA
Refer to the directions on the cover page to determine whether this section applies to the treatment works.
Effluent Testing: 1.0 mgd and Pretreatment Works. If the treatment works has a design flow greater than or equal to 1.0 mgd or it has (or is required
to have) a pretreatment program, or is otherwise required by the permitting authority to provide the data, then provide effluent testing data for the following
pollutants. Provide the indicated effluent testing information and any other information required by the permitting authority for each outfall through which
effluent is discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected
through analyses conducted using 40 CFR Part 136 methods. In addition, these data must comply with QA/QC requirements of 40 CFR Part 136 and
other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. Indicate in the blank rows provided below
any data you may have on pollutants not specifically listed in this form. At a minimum, effluent testing data must be based on at least three pollutant
scans and must be no more than four and one-half years old.
Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.)
POLLUTANT
MAXIMUM DAILY DISCHARGE
AVERAGE DAILY DISCHARGE
_V
ANALYTICAL
METHOD
MUMDL
Conc.
Units
Mass
Units
Conc.
Units
Mass
Units
Number
of
Samples
METALS (TOTAL RECOVERABLE), CYANIDE, PHENOLS, AND HARDNESS.
ANTIMONY
ARSENIC
0.010
Mg/I
0.010
Mg/I
3
BERYLLIUM
CADMIUM
0.001
Mg/I
0.001
Mg/I
3
CHROMIUM
0.005
Mg/l
0.005
Mg/I
3
COPPER
0.067
Mg/I
0.033
Mg/I
3
LEAD
0.005
Mg/I
0.005
Mg/l
3
MERCURY
0.0002
Mg/l
0.0002
Mg/I
3
NICKEL
0.010
Mg/I
0.010
Mg/I
3
SELENIUM
0.010
Mg/I
0.010
Mg/I
3
SILVER
0.005
Mg/I
0.005
Mg/I
3
THALLIUM
ZINC
0.080
Mg/I
0.044
Mg/I
3
CYANIDE
0.042
Mg/I
0.010
Mg/I
3
TOTAL PHENOLIC
COMPOUNDS
HARDNESS (as CaCO3)
_LLUse
this space (or a separate sheet) to provide information on other metals requested by the permit writer
4
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 10 of 22
FACILITY NAME AND PERMIT NUMBER:
ANDREWS WWTP, NCO020800
PERMIT ACTION REQUESTED:
RENEWAL
RIVER BASIN:
HIWASSEE
Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.)
POLLUTANT
MAXIMUM DAILY'yDISCHARGE
AVERAGE•DAILY DISCHARGE= `
ANALYTICAL
M ETHOD
' MLIMDL
Cont s..
Units
. Mass `
.,,Units '
Coni ,,
Unrts
Mass .
.Units
Number
..of
_Samples
VOLATILE ORGANIC COMPOUNDS
ACROLEIN
ACRYLONITRILE
BENZENE
BROMOFORM
CARBON
TETRACHLORIDE
CHLOROBENZENE
CHLORODIBROMO-
METHANE
CHLOROETHANE
2-CHLOROETHYLVINYL
ETHER
CHLOROFORM
DICHLOROBROMO-
METHANE
1,1-DICHLOROETHANE
1,2-DICHLOROETHANE
TRANS-1,2-DICHLORO-
ETHYLENE
1,1-DICHLORO-
ETHYLENE
1,2-DICHLOROPROPANE
1,3-DICHLORO-
PROPYLENE
ETHYLBENZENE
METHYL BROMIDE
METHYL CHLORIDE
METHYLENE CHLORIDE
1,1,2,2-TETRA-
CHLOROETHANE
TETRACHLORO-
ETHYLENE
TOLUENE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 11 of 22
FACILITY NAME AND PERMIT NUMBER:
I
ANDREWS WWTP, NCO020800
PERMIT ACTION REQUESTED:
RENEWAL
RIVER BASIN:
HIWASSEE
Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.)
_s
POLLUTANT-
'= MAXIMUM DAILY.DISCHARGE
� :"
AVERAGE DAILY -DISCHARGE'
ANALYTICAL
METHOD"
�ryL7MDL_
Cont
Units
Mass
'Units ',:Cont
,`;
Unit§
Mass_
� Units "'
Number
�, of ��
Samples
TRICHLOROETHANE
TRICHLOROETHANE TRICHLOROETHANE
TRICHLOROETHYLENE
VINYL CHLORIDE
Use this space (or a separate sheet) to provide information on other volatile organic compounds requested by the permit writer
ACID -EXTRACTABLE COMPOUNDS
P -CHLORO -M -CRESOL
2 -CHLOROPHENOL
2,4-DICHLOROPHENOL
2,4 -DIMETHYLPHENOL
4, 6 -DIN ITR O -O -C R ES O L
2,4-DINITROPHENOL
2-NITROPHENOL
4-NITROPHENOL
PENTACHLOROPHENOL
PHENOL
2,4,6 -
TRICHLOROPHENOL
Use this space (or a separate sheet) to provide information on other acid -extractable compounds requested by the permit writer
BASE -NEUTRAL COMPOUNDS
ACENAPHTHENE
ACENAPHTHYLENE
ANTHRACENE
BENZIDINE
BENZO(A)ANTHRACENE
BENZO(A)PYRENE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 12 of 22
FACILITY NAME AND PERMIT NUMBER:
ANDREWS WWTP, NCO020800
PERMIT ACTION REQUESTED:
RENEWAL
RIVER BASIN:
HIWASSEE
Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.)
POLLUTANT "
;e.
MAXIMUMrDAILY;DISCHARGE ""
::AVERAGE" DAILY"`DISCHARGE
ANALYTICAL
METHOD
T
MGMD'L
Cont. _
Units
" Mass
�. ,.
"-Units=
Conc;
Units -
Mass`
�
Units
.. Number '
of
Samples
3,4 BENZO-
FLUORANTHENE
BENZO(GHI)PERYLENE
BENZO(K)
FLUORANTHENE
BIS (2-CHLOROETHOXY)
METHANE
BIS (2-CHLOROETHYL)-
ETHER
BIS (2-CHLOROISO-
PROPYL)ETHER
BIS (2-ETHYLHEXYL)
PHTHALATE
4-BROMOPHENYL
PHENYLETHER
BUTYL BENZYL
PHTHALATE
2 -CHLORO -
NAPHTHALENE
4-CHLORPHENYL
PHENYLETHER
CHRYSENE
DI -N -BUTYL PHTHALATE
DI-N-OCTYL PHTHALATE
DIBENZO(A,H)
ANTHRACENE
1,2 -DICHLOROBENZENE
1,3 -DICHLOROBENZENE
1,4 -DICHLOROBENZENE
3,3-DICHLORO-
BENZIDINE
DIETHYL PHTHALATE
DIMETHYL PHTHALATE
2,4-DINITROTOLUENE
2,6-DINITROTOLUENE
1,2 -DIPHENYL -
HYDRAZINE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 13 of 22
F,ACILITY NAME AND PERMIT NUMBER:
ANDREWS WWTP, NCO020800
PERMIT ACTION REQUESTED:
RENEWAL
RIVER BASIN:
HIWASSEE
Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.)
=POLLUTANT
'MAXIMUM & IL DISCHARGE
"
' "AVERAGE DAILY' DISCFIARGE
�ANALYTICAL.�
METHOD
ML/MDL
Conc.
Units J
Mass.,
Units "'
t
Conc`
, U,nIts;;
'Mass
'Units
Nu(nber
;of
Samples ;
FLUORANTHENE
FLUORENE
HEXACHLOROBENZENE
HEXACHLORO-
BUTADIENE
HEXACHLOROCYCLO-
PENTADIENE
HEXACHLOROETHANE
INDENO(1,2,3-CD)
PYRENE
ISOPHORONE
NAPHTHALENE
NITROBENZENE
N-NITROSODI-N-
PROPYLAMINE
N-NITROSODI-
METHYLAMINE
N -N ITROSO D (-
PHENYLAMINE
PHENANTHRENE
PYRENE
1,2,4-
TRICHLOROBENZENE
Use this space (or a separate sheet) to provide information on other base -neutral compounds requested by the permit writer
Use this space (or a separate sheet) to provide information on other pollutants (e.g., pesticides) requested by the permit writer
.END -OF PART :D'
REFER'TO�THE'APPLICAI`IO.N.OVERVIEW (PAGE'1) TO:DETERMIN'E WHICH OTHER PARTS
OFfORM 2A YOU MUST COMPLETE,'.
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 14 of 22
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
ANDREWS WWTP, NCO020800
RENEWAL
HIWASSEE
':SUPPLEMENTAL APPLICATION INFORMATION _
PARTE TOXICITY TESTING. DATA
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points: 1) POTWs with a design flow rate greater than or equal to 1.0 mgd; 2) POTWs with a pretreatment program (or those that are
required to have one under 40 CFR Part 403); or 3) POTWs required by the permitting authority to submit data for these parameters.
• At a minimum, these results must include quarterly testing for a 12 -month period within the past 1 year using multiple species (minimum of two
species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results
show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation, if one was conducted.
• If you have already submitted any of the information requested in Part E, you need not submit it again. Rather, provide the information
requested in question EA for previously submitted information. If EPA methods were not used, report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E.
If no biomonitoring data is required, do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
complete.
E.I. Required Tests. * POLLUTANT ANALYSIS SCAN RESULTS ATTACHED
Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
❑ chronic ❑ acute
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one
column per test (where each species constitutes a test). Copy this page if more than three tests are being reported.
Test number: Test number: Test number:
a. Test information.
Test Species & test method number
Age at initiation of test
Outfall number
Dates sample collected
Date test started
Duration
b. Give toxicity test methods followed.
Manual title
Edition number and year of publication
Page number(s)
c. Give the sample collection method(s) used. For multiple grab samples, indicate the number of grab samples used.
24 -Hour composite
Grab
d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection
After dechlorination
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 15 of 22
FACILITY NAME AND PERMIT NUMBER:
ANDREWS WWTP, NCO020800
PERMIT ACTION REQUESTED:
RENEWAL
RIVER BASIN:
HIWASSEE
Test number: Test number: Test number:
e. Describe the point in the treatment process at which the sample was collected.
Sample was collected:
f. For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both
Chronic toxicity
Acute toxicity
g. Provide the type of test performed.
Static
Static -renewal
Flow-through
h. Source of dilution water. If laboratory water, specify type; if receiving water, specify source.
Laboratory water
Receiving water
i. Type of dilution water. If salt water, specify "natural' or type of artificial sea salts or brine used.
Fresh water
Salt water
j. Give the percentage effluent used for all concentrations in the test series.
k. Parameters measured during the test. (State whether parameter meets test method specifications)
pH
Salinity
Temperature
Ammonia
Dissolved oxygen
I. Test Results.
Acute:
Percent survival in 100%
effluent
%
%
%
LC50
95% C.I.
%
%
%
Control percent survival
%
%
%
Other (describe)
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 16 of 22
F/WILITY NAME AND PERMIT NUMBER:
ANDREWS WWTP, NCO020800
PERMIT ACTION REQUESTED:
RENEWAL
RIVER BASIN:
HIWASSEE
Chronic:
NOEC
%
%
%
IC25
Control percent survival
%
%
%
Other (describe)
m. Quality Control/Quality Assurance.
Is reference toxicant data available?
Was reference toxicant test within
acceptable bounds?
What date was reference toxicant test
run (MM/DD/YYYY)?
Other (describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ❑ No If yes, describe:
EA. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information, or information regarding the
cause of toxicity, within the past four and one-half years, provide the dates the information was submitted to the permitting authority and a summary
of the results.
Date submitted: / / (MM/DD/YYYY)
Summary of results: (see instructions)
END bF PART E.
REFER TQ,THE.APPLICATION' OVERVIEW (PAGE '1), TO.DETERMINE WHICKOTHER PARTS
OF FORM 2A YOU MUST'C.OMPLETE.
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 17 of 22
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
ANDREWS WWTP, NCO020800
RENEWAL
HIWASSEE
SUPPLEMENTAL APPLICATION `INFORMATION"
PART F:INDUSTRIAL USERIDISCkiARGES ANp'RCRA/CERCLA WASTES.,
All treatment works receiving discharges from significant industrial users or which receive RCRA,CERCLA, or other remedial wastes must
complete part F.
GENERAL INFORMATION:
F.I. Pretreatment program. Does the treatment works have, or is subject ot, an approved pretreatment program?
® Yes ❑ No
F.2. Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works.
a. Number of non-categorical.SlUs. 0
b. Number of CIUs. 1
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU. If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU:
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
Name: TEAM INDUSTRIES INC.
Mailing Address: 3750 AIRPORT RD.
ANDREWS, NC. 28901
F.4. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
METAL FINISHING ELECTROPLATING AND ANCILLARY PROCESSES
F.5. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
Principal product(s): GEAR CASES
Raw material(s): ALUMINUM AND OTHER MATERIALS/METALS
F.6. Flow Rate.
a. Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent.
*90.000 gpd ( continuous or X intermittent) *PERMITTED FLOW
b. Non-process wastewater flow rate. Indicate the average daily volume of non-process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent.
25.000 gpd ( continuous or X intermittent)
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following:
a., Local limits ® Yes ❑ No
b. Categorical pretreatment standards ® Yes ❑ No
If subject to categorical pretreatment standards, which category and subcategory?
40 CFR 433
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 18 of 22
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
ANDREWS WWTP, NCO020800
RENEWAL
HIWASSEE
F.8. Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e.g.,
upsets, interference) at the treatment works in the past three years?
❑ Yes ® No If yes, describe each episode.
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE:
F.9. RCRA Waste. Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe?
❑ Yes ® No (go to F.12)
F.10. Waste transport. Method by which RCRA waste is received (check all that apply):
❑ Truck ❑ Rail ❑ Dedicated Pipe
FA 1. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units).
EPA Hazardous Waste Number Amount Units
CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
mi
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
F.12. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
❑ Yes (complete F.13 through F.15.) ® No
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to origniate in
the next five years).
F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received). Include data on volume and concentration, if
known. (Attach additional sheets if necessary.)
F.15. Waste Treatment.
a. Is this waste treated (or will be treated) prior to entering the treatment works?
❑ Yes ❑ No
If yes, describe the treatment (provide information about the removal efficiency):
b. Is the discharge (or will the discharge be) continuous or intermittent?
❑ Continuous ❑ Intermittent If intermittent, describe discharge schedule.
END OF .PART F.
REFER, TO THE APP:L-ICATION,OVERVIEW;(PAGE 1) TO DETERMINE WHICH .OTHER PARTS.
OF FORM 2A YOU-MU,S;T COMPLETE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 19 of 22
Name and Address of Facility:
Town of Andrews WWTP
P.O. Box 1210
Andrews. N. C. 28901
Long Term Monitoring Plan Samples-EfFluent
Earth EovlrooM60181 Sorvlces
Michael Ladd
75 Bison Lane
Murphy, NC 28906
Robbinsville Lab (828) 479-6428
Madison County Lab (828) 649-9250
Residence (828) 837-9543
Date: 3/10/2015
Sample Collector: MB
Date and Time Sample Received: 2/17-20/15 See
Date and Time Sample Collected: 2/16-20/15 C.O.C.
Date and Time Sample Analyzed: < MHT
Test
2/16-17/15
2/17-18/15
2/18-19/15
2/19-20/15
Report Limit
Method
Units
BOD
35.1
19.2
13.6
17.3
2.0
SM5210B
mg/L
TOTAL SUSPENDED RESIDUE
25.0
22.8
16.3
18.3
1.0
SM2540D
mg/L
pH
6.7
6.6
6.5
6.3
0-14
SM4500H+
units
AMMONIA NITROGEN
4.1
2.6
2.8
2.4
0.1
SM4500NH3F
mg/L
TEMPERATURE
9.4
8.2
6.4
6.3
0.1
SM2550B
deg C
CYANIDE TOTAL
< 0.005
< 0.005
< 0.005
< 0.005
0.005
SCASM4500 CN E
mg/L
CADMIUM
< 0.001
< 0.001
< 0.001
< 0.001
0.0010
SCA -EPA 200.7
mg/L
CHROMIUM
< 0.005
< 0.005
< 0.005
< 0.005
0.005
SCA -EPA 200.7
mg/L
COPPER
0.008
0.006
0.006
0.006
0.001
SCA -EPA 200.7
mg/L
LEAD
< 0.005
< 0.005
< 0.005
< 0.005
0.005
SCA -EPA 200.7
mg/L
NICKEL
< 0.010
< 0.010
< 0.010
< 0.010
0.010
SCA -EPA 200.7
mg/L
ZINC
0.042
0.032
0.032
0.033
0.010
SCA -EPA 200.7
mg/L
SILVER
< 0.005
< 0.005
< 0.005
< 0.005
0.005
SCA -EPA 200.7
mg/L
ARSENIC
< 0.010
< 0.010
< 0.010
< 0.010
0.010
SCA -EPA 200.7
mg/L
SELENIUM
< 0.010
< 0.010
< 0.010
< 0.010
0.010
SCA -EPA 200.7
mg/L
MOLYBDENUM
< 0.010
< 0.010
< 0.010
< 0.010
0.010
SCA -EPA 200.7
mg/L
MERCURY
< 0.00020
< 0.00020
< 0.00020
< 0.00020
0.00020
SCA -EPA 200.7
mg/L
OIL & GREASE
5.1
< 5.0
< 5.0
< 5.0
5.0
SCA-EPA1664A
mg/L
N.C. Laboratory ID# 352
Analyzed By:
MB
Note: pH and Temperature performed as Field Analyses by EES
Lab Supervisor: Michael J. Ladd; EES-Robbinsville Certified By:
Name and Address of Facility:
Town of Andrews WWTP
P:O, Box 1210
Andrews.N.C. 28901
Long Term Monitoring Plan Samples -Effluent
Eorlh EoviroomWol Smicos
Michael Ladd
75 Bison Lane
Murphy, NC 28906
Robbinsville Lab (828) 479-6428
Madison County Lab (828) 649-9250
Residence (828) 837-9543
Date: 6/16/2014
Sample Collector:
MB
Date and Time Sample Received:
5/20-23/14 See
Date and Time Sample Collected:
5/19-23/14 C.O.C.
Date and Time Sample Analyzed:
< MHT
:Test
5119-20/14 5/20-21/14
5121-22/14
5/22-23/14
Report Limit Method
Units
;BOD
23.1 16.6
16.9
15.4
2.0 SM5210B
mg/L
TOTAL SUSPENDED RESIDUE
14.3 8.1
8.5
8
1.0 SM2540D
mg/L
1pH
6.6 6.1
6.1
6.0
0-14 SM4500H+
units
AMMONIA NITROGEN
5.1 32
2.8
2.2
0.1 SM4500NH3F
mg/L
TEMPERATURE
17.8 16.5
17.2
18.2
0.1 SM2550B
deg C
CYANIDE TOTAL
0.006 0.006
0.015
0.023
0.005 scasM4500 CN E
mg/L
CADMIUM
< 0.001 < 0.001
< 0.001
< 0.001
0.0010 SCA -EPA 200.7
mg/L
CHROMIUM
< 0.005 < 0.005
< 0.005
< 0.005
0.005 SCA -EPA 200.7
mglL
COPPER
0.067 0.007
0.007
0.008
0.010 SCA -EPA 200.7
mg/L
LEAD
< 0.005 < 0.005
< 0.005
< 0.005
0.005 SCA -EPA 200.7
mg/L
NICKEL
< 0.010 < 0.010
< 0.010
< 0.010
0.010 SCA -EPA 200.7
mg/L
ZINC
0.037 0.037
0.036
0.043
0.030 SCA -EPA 200.7
mg/L
SILVER
< 0.005 < 0.005
< 0.005
< 0.005
0.005 SCA -EPA 200.7
mg/L
ARSENIC
< 0.010 < 0.010
< 0,010
< 0-010
0.010 SCA -EPA 200.7
mg/L
SELENIUM
< 0.010 < 0.010
< 0.010
< 0.010
0.010 SCA -EPA 200.7
mg/L
MOLYBDENUM
< 0.010 < 0.010
< 0.010
< 0.010
0.010 SCA -EPA 200.7
mg/L
MERCURY
< 0.00020 < 0.00020
< 0.00020
< 0.00020
0.00020 SCA -EPA 200.7
mg/L
OIL & GREASE
< 5.0 < 5.0
< 5.0
< 5.0
5.0 SCA-EPA1664A
mg/L
N.C. Laboratory ID# 352
Analyzed By:
MB
Nate: pH and Temperature performed as Field Analyses by EES
Lab Supervisor Michael d_ Ladd; EES-Robbinsville Certified By:
Name and Address of Facility:
Town of Andrews WWTP
P.O. Box 1210
Andrews. N.C. 28901
Lona Term Monitorina Plan SamDIes-Effluent
Ear#6 EovifonmeoW Services
Michael Ladd
75 Bison Lane
Murphy, NC 28906
Robbinsville Lab (828) 479-6428
Madison County Lab (828) 649-9250
Residence (828) 837-9543
Date: 3/4/2013
Sample Collector: MB
Date and Time Sample Received: 2/5-8/13 See
Date and Time Sample Collected: 2/4-8/13 C.O.C.
Date and Time Sample Analyzed: < MHT
Test
2/4-5/13
2/5-6/13
2/6-7/13
217-8/13
Report Limit
Method
Units
BOD
21.8
35.6
25.1
11.5
2.0
SM5210B
mg/L
TOTAL SUSPENDED RESIDUE
7.0
21.0
14.5
< 5.0
1.0
SM2540D
mg/L
pH
6.5
6.7
6.4
6.2
0-14
SM4500H+
units
AMMONIA NITROGEN
1.4
2.1
1.6
0.9
0.1
SM4500NH3F
mg/L
TEMPERATURE
11.5
11.0
10.7
11.7
0.1
SM2550B
deg C
CYANIDE TOTAL
< 0.0050
< 0.0050
< 0.0050
< 0.0050
0.0050
SCASM4500 CN E
mg/L
CADMIUM
< 0.0010
< 0.0010
< 0.0010
< 0.0010
0.0010
SCA -EPA 200.7
mg/L
CHROMIUM
< 0.0050
0.012
0.0055
< 0.0050
0.0050
SCA -EPA 200.7
mg/L
COPPER
< 0.010
< 0.010
< 0.010
< 0.010
0.010
SCA -EPA 200.7
mg/L
LEAD
< 0.0050
< 0.0050
< 0.0050
< 0.0050
0.0050
SCA -EPA 200.7
mg/L
NICKEL
< 0.010
< 0.010
< 0.010
< 0.010
0.010
SCA -EPA 200.7
mg/L
ZINC
< 0.030
0.042
< 0.030"
< 0.030
0.030
SCA -EPA 200.7
mg/L
SILVER
< 0.0050
< 0.0050
< 0.0050
< 0.0050
0.0050
SCA -EPA 200.7
mg/L
ARSENIC
< 0.010
< 0.010
< 0.010
< 0.010
0.010
SCA -EPA 200.7
mg/L.
SELENIUM
< 0.010
0.010
< 0.010
< 0.010
0.010
SCA -EPA 200.7
mg/L
MOLYBDENUM
< 0.010
< 0.010
< 0.010
< 0.010
0.010
SCA -EPA 200.7
mg/L
MERCURY
< 0.00020
< 0.00020
< 0.00020
< 0.00020
0.00020
SCA -EPA 200.7
mg/L
OIL & GREASE
< 5.0
< 5.0
< 5.0
< 5.0
5.0
SCA-EPA1664A
mg/L.
N.C. Laboratory ID# 352 Analyzed By: MB
Note: pH and Temperature performed as Field Analyses by EES
Lab Supervisor: Michael J. Ladd; EES-Robbinsville Certified By:
aENIZ
3r- '
`N `
Environmental Testing Solutions, Inc.
March 25, 2016
Mr. Michael Ladd
Earth Environmental Services
75 Bison Lane
Murphy, NC 28906
RE: ETS PROJECT NUMBER: 11291
Dear Mr. Ladd:
PO Box 7565
Asheville, NC 28802
Phone: (828) 350-9364
Fax: (828)350-9368
Enclosed are toxicity test results for samples from the Andrews WWTP received by
Environmental Testing Solutions, Inc. March 08 through March 10, 2016.
Parameter Test Procedure EPA Method Final
Code Number Result
North Carolina Ceriodaphnia Chronic Effluent
TGP3B Toxicity Procedure EPA -821-R-02-013 PASS
(Ceriodaphnia Pass/Fail Toxicity Test)
If this test was performed as an NPDES requirement or by Administrative Letter, please enter a
P on the Effluent Discharge Monitoring Form (MR -1) for the collection date
March 08, 2016 using the parameter code TGP3B.
Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT -3) by
April 30, 2016.
If you have any questions concerning these results, please feel free to contact me.
Sincerely,
tm 1.
Laboratory Director
This report should not be reproduced, except in its entirety, without the written consent of Environmental Testing Solutions, Inc.
The results in this report relate only to the samples submitted for analysis.
North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
r t 1 WN WJ
qp rr�
k �
Environmental Testing Solutions, Inc.
Effluent Aquatic Tnxicitv Rennrt Fnrm _ Phonp IT C'hrnn;v
PO Box 7565
Asheville, NC 28802
Phone: (828) 350-9364
Fax: (828) 350-9368
Date: March 25, 2016
Facility: Earth Environmental Services NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee
Andrews NN'NVTP
Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: e. - - Project: 11291
Samples: 160308.15, 160310.13
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center rStart date: End date: Start time: End time:
Raleigh, NC 27699-1621 03-09-16 03-16-16 1348 0625
Sample Information
Collection start date:
Grab:
Composite duration:
Alkalinity (mg/L CaCO;):
Hardness (mg1L CaCO3):
Conductivity (µmhos/cm):
Total residual chlorine (mg/L):
Sample Temp. at Receipt (°C):
Sample 1
Sample 2
Control
03-07-16
03-09-16
_-
13%
13%
13%
2425-h
25-h
Control
7.38
7.17
30, 31, 32
-'
45, 42, 37
251
243
119. 152.156
< 0.10
<0.10
7.14
1.1
0.9
7.6
Test Information
Treatment:
Initial pH (SU):
Final pH (SU):
Initial DO (mg/L):
Final DO (mg/L):
Initial Temp. (°C):
Final Temp. (°C):
Organism Number
Control OrLranisms 1 2 3 4 i f, 7 R 9 In 11 V)
dart
R-11
R-12
Stut
Rwm,al l
Rmml2
13%
13%
13%
Control
Control
Control
7.38
7.17
7.08
7.51
7.31
7.08
7.16
7.13
7.10
7.16
7.14
7.05
7.6
7.6
8.0
7.8
7.7
7.8
7.8
7.9
7.9
7.8
7.8
7.8
25.0
25.3
24.8
24.8
24.8
24.9
25.1
25.1
25.1
25.1
24.9
25.0
Number of Young Produced
129
130129
131
27
128
131129
127
129 126
130
1
Adult Survival: (L)ive, (D)ead
L
L L
L
L
L
L
L L
L L
L
288
Effluent Percentage 13
Treatment 7 Orunnicmc 1 d S 4 '7 v o in r r r)
Number of Young Produced
132 35
36 35
34
34
35
133
133 32
134 36 34.1
Adult Survival: (L)ive, (D)ead
I L L
L L
L
L
L
I L
I L L
I L L -18.2
:n Kcauctlon
Effluent Percentage=
reatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan
lumber of Young Produced
Ault Survival: (L)ive, (D)ead
%Reduc c
Effluent Percentage=
rearmenr w Organisms 1 % s 4 6 7 8 9 10 11 12 Mean
lumber of Young Produced
ldult Survival: (L)ive, (D)ead
Redutti
Effluent Percentage=
'reatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mr,n
lumber of Young Produced
Ault Survival: (L)ive, (D)ead
.6 Rcdueiie
Effluent Percentage=
'reatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean
lumber of Young Produced
tdult Survival: (L)ive, (D)ead
KCaucllan
Overall Analysis:
Result: PASS LOEC: >13% NOEC: 13% ChV: >13%
DWO-form AT -3 (8/91) Rev. 11195
Chronic Test Results
Reproduction LOEC:
Final Control Mortality (%):
0.0
% Control with 3rd Broods:
100
Control Reproduction CV:
5.5
48 Hour Mortality
Method:
Control:
0
of
17
IWC:
0
of
17
Significant.:
No
Final Mortality Sibmificant at: No concentration
Reproduction Analyses
Reproduction LOEC:
>13%
Reproduction NOEL:
13%
Overall Method:
Homoscedastic t
Normal Distribution:
Yes
Method:
Shapiro -Wilks
Statistic:
0.950
Critical Value:
0.884
Equal Variances:
Yes
Method:
F -Test
Statistic:
1,323
Critical Value:
5.320
Non -Parametric Analysis (if applicable)
Method:
Effluent % Rank Sum
Critical Sum
13%
;
-T�— Page 1 of 1
• North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia
(EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Control Bench Sheet
Control #: Date: 03-09-16
Test Grouping Information: Test Organism Information:
Facility
Project #
6
Age:
5 kA4 UrN i
11?A�
4w6�nW1 A
(►?
3 UOUrntLjo
Replicate #
2 P
X11
11 - Control
�1 ai
Daily Renewal Information:
Organism Source:
In-house Culture
Test Initiation, renewal, feeding or
termination
Age:
< 24 -hours old
YWT
Batch
Source (culture board):
03.U"16 A
Time Analyst
Replicate #
1 2 3 4 5
6 7 8W91F12Culture
board cup #
�1 ai
c{Q a
Date and time organisms were
born between:
0b-OR•110 01022 ID 130'L
Average transfer volume:
0.0121 mL
din
Feeg it,/
Transfer bowl information:
pH (S.U.):
_.6
Temperature (°C):
Z,� •�
Day
Date
Test Initiation, renewal, feeding or
termination
SSW Batch
Selenastrum
Batch
YWT
Batch
Location
Incubator/Shelf
Randomizing
Template
Time Analyst
0
03-09-16
initiation F d g
03-03-16
0'L''L\�1b
�iS•Ib
2GZ
�e�
1
03-10-16
din
Feeg it,/
t
_.6
L_
L
2
03- - 6
Renews
Control, SSW
Conductivity (µmhos/cm)
(� q-
,
Number of broods present
3
03-12-16
Feeding
F
S `_yc�
"f
3 0
1
v t
4
03-13-16
Feeding
77
*Hardness (mg/LCaCO3)
4
ir
5
03-14-16
Renewal Z3 ti ng
+Analyst ide 66F 1 f r -1
*Temperature (°C)
d�....o,-r
TA'.
—LS, 1
na li
6
03-15-16
Feed: 4pa _.
Adult mortality
I(L
s �a
« 1 a x
7
03-16-16
Termination
S
t a
a. ai
,1 1
Chemical Analyses:
Initiation
Initial Final
Renewal One
Initial Final
Renewal Two
Initial Final
Concentration
Analyst
4
5
sw
Iva
NG
9
10
pH (S.U.)
'}
'1.1 b
1.31
%!q
7-09
'T,QS
Dissolved oxygen (mg/L)
_.6
L_
L
7,9
-7,9
79
Control, SSW
Conductivity (µmhos/cm)
(� q-
,
Number of broods present
t
! P w s
*Alkalinity (mg/L /LC
3 0
Lai
.3 Z
s emrg!"
kt! dL
77
*Hardness (mg/LCaCO3)
4
ir
1-1-S
15
+Analyst ide 66F 1 f r -1
*Temperature (°C)
d�....o,-r
TA'.
—LS, 1
na li
-Z.�•G,
2N,
is O
........................ ...o,. . "o,P..,aw.ca pcummw UL uLc UILLO UI Lest Im Omlon, renewal or termination by
the analyst identified in the Daily Renewal Information table. Alkalinity and hardness performed by the analysts indentified on the test bench sheets and transcribed to this bench sheet.
Survival and Renroduction Dntnr
Day
Observations
Replicate number
1
2
3
4
5
6
7
8
9
10
11
12
2
Renewal One
Adult mortality
(L = Live, D = dead)
I—
�L_
L_
L
,
Number of broods present
2
Z
L
L
Lai
7T
77
5
Numberofyoung produced
ir
1-1-S
15
l�
l�
1"`
12
IS
12
die
Renewal Two
Adult mortality
I(L
= Live, D = dead)
Number of broods present
1
1
(
1
l
1
I
Number of young produced
1 (o
t(4
110
110
13
7
Final
Total young produced
G
�? \
�o
Z \
AZ..
�i
T,
—LE
3`
-tR
Z7
p
l
t
Z b
�O
Final adult mortality
(L = Live, D = dead)
X for 3rd Broods
X
I_untrof ficceprance Lrfteria:
• of Male Adults (<_ 20%)
• Adults having 3rd Broods (>_ 80%)
I V
Mortality (<_ 20%)
Mean Offspring/Female
(>_ 15 offspring/surviving female)
% CV (< 40%)
SOP AT17 - Fxhihit AT1 7 4 I -A6.,., t i — _1 n
T74"'
Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia
(EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Test Bench Sheet
Paired with Control #I Date: w-1nvto
Client Earth Environmental Services NPDES # NCO020800
Facility Andrews WWTP outfall 001
Project # l �'L�1 \ County Cherokee
Test Concentration (Chronic Limit) . 13.0% Dilution
mL mL Total volume
preparation: Sample Dilution water mL
Samples were not aerated or treated unless otherwise noted on this form. Control, dilution water
and test renewal information are included on the Control Bench Sheet indicated above.
Chemical Analyses:
Initiation
Initial Final
Renewal One Renewal Two
Initial Final Initial -Final
Concentration
Analyst
50
§z
5W
Ab Ah N
39
261
300
Chemical Analyses:
Initiation
Initial Final
Renewal One Renewal Two
Initial Final Initial -Final
Concentration
Analyst
50
§z
5W
Ab Ah N
pH (S.U.)
-7-3 6
10
7. ) 7
7.13 7,og 7.0
Test
Concentration
Dissolved oxygen (mg/L)
,- iA
-7,Q!ii,.
% V.0 'rA4
Conductivity(µmhos/cm) a` ci t
; y` 4'r' -
*Temperature ('C) 1S -O _M> -
100%
pH(S.U,-1.
.O /
EFF
,,f . . h
Dissolved oxygen (mg/L)
Number of broods present
Conductivity (µmhos/cm)
*Total residual chlorine (mg/L) L0,10 tfl_h ? '
1
Sample number sample 1 14 ,� Sample 2
03i,- . 13
-•v, 1--11,11- w 1,..,—, 11 .ny5-1 .,.0 1vuuue1 1 ly -cam,uury. , cuiperamres periormea at me time or rest initiation, renewal or termmatton by
the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual
Chlorine Bench Sheet and transcribed to this bench sheet.
Survival and Reproduction Data (performed attest concentration) :
Day
Observations
Replicate number
1
2
3
4
5
67
8
9
10
11
12
2
Renewal One
Adult mortality
(L = Live, D = dead)
Number of broods present
1
i
5
Number of young produced
�c+
, �+
Lj1
��
c
G�1
RenewalTwo
Adult mortality
(L = Live, D = dead)
Number of broods present
\El
LE
l
11-
t
Number of young produced
n
11
lot
t
I�
is
J
Q
A
� S
�f\
16
11
I S
10
7
Final
Total young produced
32
3S
3b
35INA
W1
3S
33
33
3Z
,3A
bLo
Final adult mortality
Fk__
(L = Live, D = dead)L--
--- "-- •• •••-•-- --•••o-"'I - -• � •" ---- iii ncucm vuc la=y cl tuay al, amnp— were unuteu to me test concentration prior to Use With soft synthetic water
and warmed to 2S.0± 1.0°Cin a warm water bath.
Comments:
Test Results and Statistical Analyses: M
Test results Statistics
% Mortality
`n
V /.
Mean offspring
3il-1.
per female
% Reduction
.yea?
from control
t -Stat or
Rank -Sum
I-�J
1
1 -Tailed
Critical
PASS or FAIL
SOP AT12 - Exhibit AT12.2, revision 11-01-14
Concentration
(%)
Day
Number of oung produced by replicate number
Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test
Average
reproduction
(offspring/female)
Coefficient of
variation
(1y")
Percent reduction
from control ('Y,)
1
2
EPA-821-R-02-013, Method 1000.0 - North Carolina Modification
4
5
6
7
8
9
c ;1
11
12
Control
5
Quality Control
16
13
15
14
14
Verification of Data Entry, Calculations, and Statistical Analyses
17
12
15
12
16
Client: Andrews WWTP
28.8
5.5
Not applicable
Test dates: March 09 - 16, 2016
16
14
16
Environmental Testing Solutions, Inc.
Project number: 11291
14
17
12
Reveiwed by: j.,-"
14
14
14
Total
Concentration
(%)
Day
Number of oung produced by replicate number
Survival
M
Average
reproduction
(offspring/female)
Coefficient of
variation
(1y")
Percent reduction
from control ('Y,)
1
2
3
4
5
6
7
8
9
10
11
12
Control
5
13
16
13
15
14
14
14
17
12
15
12
16
100
28.8
5.5
Not applicable
7
16
14
16
16
13
14
17
12
15
14
14
14
Total
29
30
29
31
27
28
31
29
27
29
26
30
13%
5
15
18
17
17
18
19
16
18
15
15
19
17
100
34.1
4.0
-18.2
7
17
17
19
18
16
15
19
15
18
17
15
19
Total
32
35
36
35
34
34
35
33
33,
32
34
36
Dunnett's MSD value: 1.522 MSD = Minimum Significant Difference
PMSD: 5.3 PMSD = Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10`x' percentile) = 13%.
Upper PMSD bound determined by USEPA (90`1' percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET
Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001 b).
USEPA. 2001x, 2001b. Final Report: Interlaboratory Variability Study of' EPA Short -tern Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 -Appendix. EPA -821-13-01-004 and EPA -821-13-01-005.
US Environmental Protection Agency, Cincinnati, OH.
Andreas 03-09-16
Transform: Untransfored_ 1 Tailed
m
Conc,% ,„ Mean .F N Mean,` Mean;, Min , .Max, GV% N t,stat., Critical MSD,
.. a
D -Control 28.833 1.0000 28.833 26.000 31.000 5.500 12
13 34.083 1.1821 34.083 32.000 36.000 4.046 12 -8.654 2.508 1.522
iAuxilia�y Test§
Statistic.
" Critical'
Skew.:'_ Kurt' .j
Shapiro-Wiilk's Test indicates normal distribution (p > 0.01)
0.94975
0.884
-0.2526 -0.8427
F-Testindicates equal variances (p=0.65)
�
5.31963
M
In
Hypotliesis Test.(1=tail,-0:01). - _`_ " :. " ..
MSDu MSDp; ; ^`MSB ;,M$E, ' F=Prod `. ' .df I
HomoscedastictTestindicatesnosignificant differences
1.52174 0.05278
165.375
2.20833 1.6E-08 1,22
Ai
F :
�
;31 Environmental
Testing Solutions, Inc.
_Ceriodaptinia:Suivival
_._
and'Reprodtictibn iestSReproduction •_
Start Date:
3/9/2016
TestlD:
CdPFCRNC
Sample ID:
Andrews WVVfP
End Date:
3/16/2016
Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR -Discharge Monitoring Report
Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013
Test Species:
CD-Ceriodaphnia dubia
Comments:
C. Conc:%° 2, 3 ,' 4... 5. " 6
Y ' 7
9'. 10 .
D -Control
29.000
30.000 29.000
31.000 27.000 28.000
31.000 29.000
27.000 29.000
D -Control
26.000
30.000
13
32.000
35.000 36.000
35.000 34.000 34.000
35.000 33.000
33.000 32.000
13
34.000
36.000
Transform: Untransfored_ 1 Tailed
m
Conc,% ,„ Mean .F N Mean,` Mean;, Min , .Max, GV% N t,stat., Critical MSD,
.. a
D -Control 28.833 1.0000 28.833 26.000 31.000 5.500 12
13 34.083 1.1821 34.083 32.000 36.000 4.046 12 -8.654 2.508 1.522
iAuxilia�y Test§
Statistic.
" Critical'
Skew.:'_ Kurt' .j
Shapiro-Wiilk's Test indicates normal distribution (p > 0.01)
0.94975
0.884
-0.2526 -0.8427
F-Testindicates equal variances (p=0.65)
1.32271
5.31963
Hypotliesis Test.(1=tail,-0:01). - _`_ " :. " ..
MSDu MSDp; ; ^`MSB ;,M$E, ' F=Prod `. ' .df I
HomoscedastictTestindicatesnosignificant differences
1.52174 0.05278
165.375
2.20833 1.6E-08 1,22
Treatments vs D -Control
Andrews 03-09-16
M
h
>- Environmental Testing Solutions, Inc.
351 Depot Street
Asheville, NC 28801
Phone: (828) 350-9364
Fax: (828)350-9368
Whole Effluent Toxicity Chain -of -Custody Form
Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 13%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Composite sample: r r/,
U Start date: M 1 6 `•Y
End date:
Number of samples per hour:
Time: 2,,'Q 3 Av'%.
Time: G�-
Sample location:
Volume collected for testing:
Number of containers filled for testing:
Method of transport to laboratory:
Chilled during collection? Comments:
If chilled, specify temperature:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
Sample collected by:
Print Signature Dale and time
Relinquished by:
k
y
Print signature Dale and time
Relinquished by:
Print signature Date and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS y:
r / 3
Print signature Date and time
Custody seals intact?: =
Yes No Not used
Samples received in good condition?: =
Yes No
T4 f"'
Tracking number:
Comments:
Received by: K,LAIL WKV W
--%
{Print Signature Date and time d
Received by:
s
Print Signature Dale and time
Received at ETS bv:
�� Vim- 03, t7� ij0
�(2
Print Jlgnaturc Uale and trate
Sample temperature upon receipt at ETS (dC):
Total residual chlorine upon receipt at ETS:
(DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Absent
Project number: I UA I Sample number: %U0.30A Its
7--
-q
Environmental Testing Solutions, Inc.
351 Depot Street
Asheville, NC 28801
Phone: (828) 350-9364
Fax: (828) 350-9368
Whole Effluent Toxicity Chain -of -Custody Form
Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Ceriodaphuia dubia Effluent dilution: 13%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Imple mtormation: (to oe completea Dy sample couector)
Composite sample: ,r� MI- -110 mple location: C CS`' �l� `L tl �,l
Start date: 3 Time: Vel S R Volume collected for testing: 1 l.r e'l
End date: Time: Number of containers filled for testing:
Number of samples per hour: 1 -e- Method of transport to laboratory:
Chilled during collection? 1.l !e S Comments:
If chilled, specify temperature: 1 "
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
Sample collected by:
lv-�l 31161,114n
Print Signature Date and time
Relinquished by: Receival by:
f
ti
Print Signature Dale and time Print Signature Date and time P
Relinquished by: Received by:
jX
C
Print Signature Date and time Print Signature Dale and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: R
-:WC)IIL�-
L I I 132
Print Signature Date and time
Custody seals intact?: =
Yes No Not used
Samples received in good condition?: Ft
Yes No
Tracking number: Ji
Comments:
Received at ETS bv:
,1�
►'K�--13
�
03.►o�N�
2�
rnm aignaure -le ano ume
Sample temperature upon receipt at ETS (°C):
Q •� �Ci
Total residual chlorine upon receipt at ETS:
(DPD Presence/Absense Indicator, MDL = 0.10 ing/L) Present Absent
Project number: MAI Sample number: 1410310'13
Ov€ q
C ? rK'
,;J Environmental Testing Solutions, Inc.
1.14
1.12
1.10
1.08
1.06
1.04
1.02
2.5
2.0
z
1.5
e�
�j 1.0
.� 0.5
r
1.4
1.3
1.2
1.1
1.0
0.9
Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
USEPA Control Limits (:I:: 2 Standard Deviations)
USEPA Warning and Control Limits (75th and 90th Percentile CVs)
Laboratory Warning and Control Limits (10th and 25th Percentile CVs)
.......•..........................•..•.•..•..............•.....................................
081
0� -VA 0� 1a OA ,A Oti �A �� 03 15 0� �� 0J N$ OS ,� 09 ,� �a ,5 OA �� �5 ,5 06 ,-0� �5 0�'0 1ti `6
,ti, 0�� Otis Otis 0�
Test date
• 7 -day IC25= 25% inhibition concentration. An estimation of the concentration of sodium chloride
that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
— — Central Tendency (mean IC25)
— Warning Limits (mean IC25 Sato or SA75) q,,w
...
mm.e,
......... Control Limits (mean IC25± SA.Zs1 SA.90' or 2 Standard Deviations) 0`
s--
grpp
I
r
PO Box 7565
Asheville NC 28802
Phone: (828) 350-9364
Fax: (828)350-9368
ti.
E
Environmental Testing Solutions, Inc.
June 27, 2016
Mr. Michael Ladd
Earth Environmental Services
75 Bison Lane
Murphy, NC 28906
RE: ETS PROJECT NUMBER: 11545
Dear Mr. Ladd:
Enclosed are toxicity test results for samples from the Andrews WWTP received by
Environmental Testing Solutions, Inc. June 14 through June 16, 2016.
Parameter Test Procedure EPA Method Final
Code Number Result
North Carolina Ceriodaphnia Chronic Effluent
TGP3B Toxicity Procedure EPA -821-R-02-013 PASS
(Ceriodaphnia Pass/Fail Toxicity Test)
If this test was performed as an NPDES requirement or by Administrative Letter, please enter a
P on the Effluent Discharge Monitoring Form (MR -1) for the collection date
June 14, 2016 using the parameter code TGP313.
Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT -3) by
July 31, 2016.
If you have any questions concerning these results, please feel flee to contact me.
Sincerely,
Jimumner
Laboratory Director
This report should not be reproduced, except in its entirety, without the written consent of Environmental Testing Solutions, Inc.
The results in this report relate only to the samples submitted for analysis.
Nort11 Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600
South Carolina Certificale Number: Clean Water Act: 99053-001
PO Box 7565
Asheville, NC 28802
Phone: (828) 350-9364
Fax: (828)350-9368
Datta: June 27, 2016
Facility: Earth Environmental Services NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee
Andrews MVWTP
Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor; r A • Project: 11545
Samples: 160614.07, 160616.07
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center Start date: End date: ! Start time: End time:
Raleigh, NC 276994621 06-15-16 06-22-16 r 1230 0703
Sample Information
Collection start date:
Grab:
Composite duration:
Alkalinity (mgtL CaCO;):
Hardness (mg/L CaCO,):
Conductivity (pntltos/cm):
Total residual chlorine (mg/L):
Sample Temp. at Receipt ("C):
Sample 1
Sample 2
Control
06-13-16
06-15-16
-
13%
13%
13%
24-h
24.5 -It
-'
7.55
7.67
30
7.67
-
40
260
234
1st. tss. lsc
<0.10
i
-
1.2
0.7
7.8 i
Environmental Testing Solutions, Inc.
Effluent Aauatic
Toxicitv Report Form - Phase II Chronic Cerindnnhnin dnhin
PO Box 7565
Asheville, NC 28802
Phone: (828) 350-9364
Fax: (828)350-9368
Datta: June 27, 2016
Facility: Earth Environmental Services NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee
Andrews MVWTP
Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor; r A • Project: 11545
Samples: 160614.07, 160616.07
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center Start date: End date: ! Start time: End time:
Raleigh, NC 276994621 06-15-16 06-22-16 r 1230 0703
Sample Information
Collection start date:
Grab:
Composite duration:
Alkalinity (mgtL CaCO;):
Hardness (mg/L CaCO,):
Conductivity (pntltos/cm):
Total residual chlorine (mg/L):
Sample Temp. at Receipt ("C):
Sample 1
Sample 2
Control
06-13-16
06-15-16
-
13%
13%
13%
24-h
24.5 -It
-'
7.55
7.67
30
7.67
-
40
260
234
1st. tss. lsc
<0.10
<0_10
-
1.2
0.7
7.8 i
Test Information
Treatment:
Initial pH (SU):
Final pH (SU):
Initial DO (ing/L):
Final DO (mg/l.):
Initial Temp. ("C):
Final Temp. ("C):
Organism Number
ControlOreanisms 1 2 3 4 5 6 7 R 9 10 11 17
Stan
Rmcm1l I
Rene al 2
Sian
Rmrnal l
R.12
13%
13%
13%
Control
Control
Control
7.55
7.67
7.50:
7.67
7.75
7.81
7.70
7.77
7.61
7.71
7.86
7.56
7.8
7.8
7.8 i
7.8
7.9
7.7
7.9
8.0
7.8
7.9
8.0
7.9
24.9
25-0
24.8
24.8
24.7
24.7
252
25.1
25.0
24.7 1
24-7
24.7
Number of Young Produced
126
29
25
25
29
25
26
26
27
28
26
27
28.9
Adult Survival: (L)ive, (D)ead
I L
L
L
L
L
L
L
L
L
L
L
L
26.6
Effluent Percentage 13%
Trentment2 Ortranicmc 1 7 d S A 7 2 0 In 17 17
Number of Young Produced
132
29
29
30
27
29
30
28
28
27
29
29
28.9
Adult Survival: (L)ive, Mead
I L
L
L
L
L
L
L
L
L
L
L
L
-8.8
-r. RcUM-
Effluent Percentage=
reatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 UeaR
lumber of Young Produced -H
Ault Survival: (L)ive, (D)ead I
c6 Rcdunis
Effluent Percentage=
"reatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Nieaa
(umber of Young Produced
AWL Survival: (L)ive, (D)ead
3. Reductic
Effluent Percentage=
'reatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 �lcm
(umbel- of Young Produced
,dull Survival: (L)ive, (D)ead
%Redunic
Effluent Percentage=
•reatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 ' dlean
lumber of Young Produced
Ault Survival: (L)ive, (D)ead
Overall Analysis:
Result: PASS LOEC: >13% NOEC: 13% ChV: >13%
DIYO form AT -3 (8/91) Rev. 11195
Chronic Test Results
Reproduction LOEC:
Final Control Monality (%):
0.0
% Control with 3rd Broods:
100
Control Reproduction CV:
5.4
48 Hour Mortality
Method:
Control:
0
of
12
IWC:
0
of
12
Significant?:
No
Final Mortality Significant at: No concentration
Reproduction Analyses
Reproduction LOEC:
>13%
Reproduction NOEC:
13%
Overall Method:
Homoscedastic t
Normal Distribution:
Yes
Method:
Shapiro -Min.'s
Statistic:
0.938
Critical Value:
0.884
Equal Variances:
Yes
Method:
F -Test
Statistic:
1.096
Critical Value:
5.320
Non -Parametric Analysis (if applicable)
k4ethod:
Effluent 9b Rank Sum
Critical Sum
13%
•
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia alubia Page 1 of 1
• (EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Controlx Bench Sheet
Control #: Date: 06-15-16
Test Grouping Information: Test Organism Information:
Daily Renewal Information:
Day Date
0 06-15-16
1 06-16-16
2 06-17-16
3 05-18-16
4 06-19-16
5 06-20-16
6 06-21-16
7 1 06-22-16
Organism Source:
Facility
Project #
6
Source (culture board):
6"11-041
5
tpmweiZul1
Culture board cup #
4
F Vt,\ N
b
3
g l.Y tL
1 S
pH (S.U.): -'j • A�t
Temperature ('Q: -tS•`
2
1-
Control`
7,7/
Daily Renewal Information:
Day Date
0 06-15-16
1 06-16-16
2 06-17-16
3 05-18-16
4 06-19-16
5 06-20-16
6 06-21-16
7 1 06-22-16
Organism Source:
In-house Culture
Age:
a 24 -hours old
Source (culture board):
6"11-041
Replicate #
1121314 5 6: 7 8 9 10 'il 12
Culture board cup #
Sig 110 it tj tF t li
Date and time organisms were
born between:
Otr�s-Ile OWE• `m mlo
Average transfer volume:
0.0121 mL
Transfer bowl information:
pH (S.U.): -'j • A�t
Temperature ('Q: -tS•`
SSW Batch Selenastrum ywr Location
Batch Batch Incubator/Shelf
6--%,L46 oS-2�•►� o5-vq-►16. I -M-ls
Chemical Analyses:
Initiation
Renewal One
Renewal Two
% Mortality (<_ 20%)
Initial
Final
Initial Final
Initial
Final
Concentration
Analyst
8
lyb
10
li
-
2
pH (S -U.)
-1 -VI
7,7/
7.7S' '� •
' ,
']'.
Dissolved oxygen (mg/L)
-1.
7,8
7.1 5-0
7 It
Conductivity (µmhos/un)
Control, SSW
) S
(L = Uve, D = dead)
I sst
5
4 y
*Alkalinity (mg/L CaCO3)
od•x7}ti z�
*Hardness (mg/LCaCO3)
y()
Number of broods present
ZLl
*Temperature (°C)
'Analyst
Z.�
Z-4•-1•1
Z-�` 1.
Zl•�
%`�.
identified for each day,performed pH; dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation, renewal or termination by
the analyst identified in the Daily Renewal Information table. Alkalinity hardness
rLZ
and performed
by the analysts
indentified on the test bench sheets
and transcribed
to this bench sheet -
Survival and Renrodurtinn nnfv"-
1t
v
L -L
Day
observations
Replicate number
O 67.
% Mortality (<_ 20%)
1
2
1 3
4
5
1 6
F 7
8
9
10
li
12
2
Adult mortality
Renewal One
(L = Uve, D = dead)
�
�
�
�
Number of broods present
ZLl
L2_Z
Z
rLZ
5
Number.of young produced
1t
v
L -L
(Z
lZ
IS
Renewal Two
Adult mortality
(L=Live, D=dead)
�--
L_
Lr
I_
`
L
Number of broods presentLl
L%
Lk`
Ll
L
I
Number of young produced
t �p
l
t
I (o
t J
l j
111A
q7
Final
Total young produced
�-
Zr1
15
Z-5
Z`l
1S
16
Zb
-M
UZ
'46
2 l
Final adult mortality
(L= Live, D= dead) .
L
X for 3rd Broods
X
% of Male Adults (5 20%)
r
% Adults having 3rd Broods (>_ 8091.)
O 67.
% Mortality (<_ 20%)
Mean Offspring/Female
(>t 15 offspring/surviving female)
.4"o
%CV (<40%)
5•�Z
SOP AT12 - Exhibit AT12.3,.revision 11-01-14
Concentration
(%)
Day
,:,'
Ceriodaphttia (labia Chronic Whole Effluent Toxicity Test
Average
reproduction
(offspring/female)
v
Coefficient of
variation
(%)
Percent reduction
from control ('%)
1
2
dui
J
EPA -821-R-02-013, Method 1000.0 - North Carolina Modification
5
6
7
8
9
10
11
12
Control
5
12
13
II
11
13
12
II
12
IS
12
13
Quality Control
100
26.6
5.4
Not applicable
7
14
16
Verification of Data Entry, Calculations, and Statistical Analyses
14
16
13
15
14
12
16
13
14
Total
26
29
Client: Andrews WWTP
25
Environmental Testing Solutions, Inc.
25
Test dates: June 15 - 22, 2016
26
27
28
26
Project number: 11545
13%
5
16
15
Reveiwed by: 5!n!=�
Concentration
(%)
Day
Number oryoun roduced by replicate number
survival
('M,)
Average
reproduction
(offspring/female)
v
Coefficient of
variation
(%)
Percent reduction
from control ('%)
1
2
3
4
5
6
7
8
9
10
11
12
Control
5
12
13
II
11
13
12
II
12
IS
12
13
13
100
26.6
5.4
Not applicable
7
14
16
14
14
16
13
15
14
12
16
13
14
Total
26
29
25
25
29
25
26
26
27
28
26
27
13%
5
16
15
12
15
11
11
15
13
12
12
12
15
100
28.9
4.8
-8.8
7
16
14
17
15
16
18
15
15
16
15
17
14
Total
32
29
29
30
27
29
30
28
28
27
29
29
Dnnnett'S MSD value: 1.445 MSD = Mininuun Significant Dirfcrcnce
I'M SD: 5.4 PMSD = Percent Mininutm Significant Difference
PMSD is a measure of test precision. The PMSD is the miniintun percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity lest.
Lower PMSD bound determined by USEPA (10'x' percentile) = 13%.
Upper PMSD bound determined by USEPA (90111 percentile) = 47%.
Lower and upper PMSD bounds were determined from the l Olh and 90th percentile, respectively, of PMSD data from EPA's WET
Interlaboratory Vanab.ility Study (tJSEPA, 2001 a: USEPA, 2001 b). _
USEPA. 200 la, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent'roxicity'I'cst Methods, Volumes I and 2 -Appendix. EPA -821-13-01-004 and L'PA-821-13-01-005.
US Environmental Protection Agency, Cincinnati, OI -I.
Anthems 06-15-M
A,
3��:&
Lj
i
.< Environmental Testing Solutions, Inc.
Ceriodaplinfa Survival and'Reproduction Test -Reproduction
Start Date:
6/15/2016
Test ID: CdPFCRNC
Sample ID:
Andrews WVVTP
End Date:
6/22/2016
Lab lD: ETS-Envir. Testing Sol.
Sample Type:
DMR -Discharge Monitoring Report
Sample Date:
Protocol: FWCHR-EPA-821-R-02-013
Test Species:
CD-Ceriodaphnia dubia
Comments:
;
D -Control
26.000
29.000 25.000 25.000 29.000 25.000
26.000 26.000
27.000 28.000
D -Control
26.000
27.000
13
32.000
29.000 29.000 30.000 27.000 29.000
30.000 28.000
28.000 27.000
13
29.000
29.000
-x i ransrorm, IJntranstormed _ : fi=Tailed
Cont % Mean N Mean Mean Mm ;_ ,Max"/o N t -Stat Critical: MSD-
rol 26.583 1.0000 26.583 25.000 29.000 5.430 12
13 28.917 1.0878 28.91.7 27.000 32.000 4.769 12 -4.049 2.508 1.445
AuxiliaryTestsCrttiCal
Shapiro--WilWs Test indicates normal distribution (p > 0.01) 0.93804 0.884 0.62647 -0.1212
F -Test indicates equal variances (p = 0.88) 1.09562 5.31963
Treatments vs D -Control
no significant differences 1A4544 0.05437 32.6667 1.99242 5.4E-04 1,
Andi•eirs 06-15-16
351 Depot Street
Asheville, NC 28801
Phone: (828) 350-9364
Fax: (828) 350-9368
Environmental Testing Solutions, Inc.
wnole mlluent Toxicity Chain -of -Custody Form
Facility: Earth Environmental Services, Inc. NPDES # NCO020800 Pipe #: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Ceriodaphida dubia Effluent dilution: 13%
Test type: Pass/Fail Chronic Parameter code: TGP31B
Sample information:
Composite sample:
S�KN���:
Sample location:
r
Startdate: -'.
Time: C,,-
Volume collected for testing:
tsar I
End date: (0- C L(4WO
Time: Odo-4-vu
Number of containers filled for testing:
t •'t`C.
Number of samples per hour:
1 S �,r, tp (�a_� S� u
L Method of transport to laboratory:
pr "m Aba-m
Chilled during collection?
y S
Comments:
If chilled, specify temperature: f "
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space
Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory.
Sample custody:
Sample collected by
ttU nt: wn,pteteu oy sample couector anu lacutty personnel)
Ir"� b� - • ; etc �c
Print $ ^nature _ Date and time
Relinquished by:
Print Signature Date and time
Received bv_
- -^ ..V.— ate and Wnc
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: 17 t
'Y -�W 1 &711 q 7 16
Q\ --all 1SoS
Prim Signature Date and time
Custody seals intact?: u
Yes No Not used
Samples received in good condition?:
Yes No
Tracking number: 0 P,
Comments:
Received at ETS by: /
S�KN���:
G to
j
- -^ ..V.— ate and Wnc
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: 17 t
'Y -�W 1 &711 q 7 16
Q\ --all 1SoS
Prim Signature Date and time
Custody seals intact?: u
Yes No Not used
Samples received in good condition?:
Yes No
Tracking number: 0 P,
Comments:
Received at ETS by: /
ti
S�KN���:
G to
tsar I
Print VSignature
Dal. and time
Sample temperature upon receipt at ETS (°.C):
t •'t`C.
Total residual chlorine upon receipt at ETS:
= Full
(DPD Presence/Absense Indicator. MDL — 0. l0 mg/L)
pr "m Aba-m
54
Projectnumber:1t Sample number: 1(00t AUT
ti
Whole Effluent Toxicity Chain -of --Custody Form
Facility: - Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Ceriodaphitia dabfa Effluent dilution: 13%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information, (to be completed by sample collector) i
Composite sample: Sample location: ,
Start date: Time: 060 1%V Volume collected for testing:
;t
J
End date: i0[[ G r( V-7 Time: 0931 A/ Number of containers tilled for testing:
Number of samples per hour: j d.,,,1(e,75O V Q : A Method of transport to laboratory:
J-
Chilled during collection?�/ eS Comments:
If chilled, specify temperature: / D
Triple rinse sample container with sample before filling. Completely fill the sample container with'no air space.
s
Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
i
Satuple collected by:=3�tv-\
Prinlsign.t= Datcand time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: '., � Received at ETS by:
Fni C �U[' Ob•Id•tlo
L. 1 l �3P'1 ��Maw 1,643
Print Sigranvc Date and lime Print sigtawrc Date and tine
Custody seals intact?: =
Ycs \0 Nei used
Samples received in good condition?; =
Yes Na
Traclting number:
Comments:
Sample temperature upon receipt at ETS (°C): v•, I, C
Total residual chlorine upon receipt at ETS:
(DPD PreseneeiAbsense Indicator. MDL = 0.10 mg1L) Ptcsent absent
Project number: Sample number: ka Q(p ((o.m- -
351 Depot Street
Asheville, NC 28801
Phone: (828) 350-9364
Fax: (828) 350-9368
Environmental Testing Solutions, Inc.
Whole Effluent Toxicity Chain -of --Custody Form
Facility: - Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Ceriodaphitia dabfa Effluent dilution: 13%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information, (to be completed by sample collector) i
Composite sample: Sample location: ,
Start date: Time: 060 1%V Volume collected for testing:
;t
J
End date: i0[[ G r( V-7 Time: 0931 A/ Number of containers tilled for testing:
Number of samples per hour: j d.,,,1(e,75O V Q : A Method of transport to laboratory:
J-
Chilled during collection?�/ eS Comments:
If chilled, specify temperature: / D
Triple rinse sample container with sample before filling. Completely fill the sample container with'no air space.
s
Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
i
Satuple collected by:=3�tv-\
Prinlsign.t= Datcand time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: '., � Received at ETS by:
Fni C �U[' Ob•Id•tlo
L. 1 l �3P'1 ��Maw 1,643
Print Sigranvc Date and lime Print sigtawrc Date and tine
Custody seals intact?: =
Ycs \0 Nei used
Samples received in good condition?; =
Yes Na
Traclting number:
Comments:
Sample temperature upon receipt at ETS (°C): v•, I, C
Total residual chlorine upon receipt at ETS:
(DPD PreseneeiAbsense Indicator. MDL = 0.10 mg1L) Ptcsent absent
Project number: Sample number: ka Q(p ((o.m- -
r Environmental Testing Solutions, Inc.
1.14
1.12
1.10
1.08
1.06
1.04
1.02
'r_XI
U
2.0
1.5
�n
U 1.0
1-4
0.5
_C
1.4
Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
USEPA Control Limits 2 Standard -Deviations)
............................................................
..............................................................................................
USEPA Warning and Control Limits (75th and 9Ott, Percentile CVs)
................................................................ ..............................
J
1.3 Laboratory Warning and Control Limits (10th and 25th Percentile CVs)
1-2
1.0
0.9 .........................................;.............................
0.8
.5X5
O�A3 XONOSA5 X06 pq �o� ,�•�Q$ pA toq ,cs 150pbp9r%o, �b �oL pq �oL pq lob p��oa p��0a p5 SO
-e"
Test date
7 -day IC,, = 25% inhibition concentration. An estimation of the concentration of sodium chloride
that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
— — Central Tendency (mean IC20
— — - Warning Limits (mean IC,5 f SA 10 or SA,,)
'Yw.4wrM b
• • • • • • • • • Control Limits (mean IC f S _ S
z�_ �.z>> a9o� or � Standard Deviations)
{=
F
4
r Environmental Testing Solutions, Inc.
1.14
1.12
1.10
1.08
1.06
1.04
1.02
'r_XI
U
2.0
1.5
�n
U 1.0
1-4
0.5
_C
1.4
Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
USEPA Control Limits 2 Standard -Deviations)
............................................................
..............................................................................................
USEPA Warning and Control Limits (75th and 9Ott, Percentile CVs)
................................................................ ..............................
J
1.3 Laboratory Warning and Control Limits (10th and 25th Percentile CVs)
1-2
1.0
0.9 .........................................;.............................
0.8
.5X5
O�A3 XONOSA5 X06 pq �o� ,�•�Q$ pA toq ,cs 150pbp9r%o, �b �oL pq �oL pq lob p��oa p��0a p5 SO
-e"
Test date
7 -day IC,, = 25% inhibition concentration. An estimation of the concentration of sodium chloride
that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
— — Central Tendency (mean IC20
— — - Warning Limits (mean IC,5 f SA 10 or SA,,)
'Yw.4wrM b
• • • • • • • • • Control Limits (mean IC f S _ S
z�_ �.z>> a9o� or � Standard Deviations)
September 23, 2016
Mr. Michael Ladd
Earth Environmental Services
75 Bison Lane
Murphy, NC 28906
RE: ETS PROJECT NUMBER: 11720
Dear Mr. Ladd:
PO Box 7565
Asheville, NC 28802
Phone: (828) 350-9364
Fax: (828) 350-9368
Enclosed are toxicity test results for samples from the Andrews WWTP received by
Environmental Testing. Solutions, Inc. September 06 through September 08, 2016.
Parameter Test Procedure EPA Method Final
Code Number Result
North Carolina Ceriodaphnia Chronic Effluent
TGP3B Toxicity Procedure EPA -821-R-02-013 PASS
(Ceriodaphnia Pass/Fail Toxicity Test) - .
If this test was performed as an NPDES requirement or by Administrative Letter, please enter a
P on the Effluent Discharge Monitoring Form (MR -1) for the collet_tion date
September 06, 2016 using the parameter code TGP3B.
Additionally,, please sign and submit the original DWQ Aquatic Toxicity Form (AT -3) by
October 31, 2016.
If you have any questions concerning these results, please feel free to contact me.
Sincerely,
QtUmrner
Laboratory Director
This report should not be reproduced,'except in its entirety, without the written consent of Environmental Testing Solutions, Inc.
The results in this report relate only to the samples submitted for analysis
North Carolina Certificate Nwnbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
0.
aa
,
Environmental Testing Solutions, Inc.
September 23, 2016
Mr. Michael Ladd
Earth Environmental Services
75 Bison Lane
Murphy, NC 28906
RE: ETS PROJECT NUMBER: 11720
Dear Mr. Ladd:
PO Box 7565
Asheville, NC 28802
Phone: (828) 350-9364
Fax: (828) 350-9368
Enclosed are toxicity test results for samples from the Andrews WWTP received by
Environmental Testing. Solutions, Inc. September 06 through September 08, 2016.
Parameter Test Procedure EPA Method Final
Code Number Result
North Carolina Ceriodaphnia Chronic Effluent
TGP3B Toxicity Procedure EPA -821-R-02-013 PASS
(Ceriodaphnia Pass/Fail Toxicity Test) - .
If this test was performed as an NPDES requirement or by Administrative Letter, please enter a
P on the Effluent Discharge Monitoring Form (MR -1) for the collet_tion date
September 06, 2016 using the parameter code TGP3B.
Additionally,, please sign and submit the original DWQ Aquatic Toxicity Form (AT -3) by
October 31, 2016.
If you have any questions concerning these results, please feel free to contact me.
Sincerely,
QtUmrner
Laboratory Director
This report should not be reproduced,'except in its entirety, without the written consent of Environmental Testing Solutions, Inc.
The results in this report relate only to the samples submitted for analysis
North Carolina Certificate Nwnbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
PO Box 7565
Asheville, NC 28802
t. iq
Phone: (828) 350-9364
N - Fax: (828) 350-9368
Environmental Testing Solutlons, Inc.
Effluent Aquatic Toxicity Report Form - Phase I1 Chronic Ceriodaphnia diubia Date: September 23, 2016
Facility: Earth Environmental Services NPDES #: NC - .0020800 Pipe #: 001 County: Cherokee
Andrews WWTP
Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor:Dutbi� Project: 11720
Samples: 160906.06, 160908.07
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh, NC 27699-1621 09-07-16 09-14-16 1010 0802
Sample Information
Collection start date:
Grab:
Composite duration:
Alkalinity (mg/L CaCO3):
Hardness (mg/L CaCO3):
Conductivity (µmhos/cm):
Total residual chlorine (mg/L):
Sample Temp. at Receipt (°C):
Sample 1
Sample 2
Control
09-05-16
09-07-16
Rcnnval 2
13%
13%
13%
24.5-h
24.5-h
-
7.67
34
E _ }
9M
40
530
385
161.160.1 9
<0.10
<010
7.81
4.5
0.9
7.8
Test Information
Treatment:
Initial ptI (SU):
Final pH (SU):
Initial DO (mg/Ly
Final DO (mg/L):
Initial Temp. (°C):
Final Temp. (°C):
Organism Number
Control Oreanisms 1 2 I S ( 7 9 O in t t ii
Start
Rcn W t
Rcnn\a12
Stan
Rrncnal 1
Rcnnval 2
13%
13%
13%
Control
Control
Control
7.67
7.50
7.59
7.52
7.49
7.53
7.78
7.69
7.63
7.63
7.81
7.64
7.9
7.8
7:9
7.9
7.8
7.9
7.9
8.0
7.9
7.9
8.0
7.9
24.9
24.7
24.9
24.8
24.9
24.8
25.0
24.9
25.1
24.8
24.8
25.0
Number of Young Produced
130 26
27
28
28 29
28 27 27
129 29
30
29
Adult Survival: (L)ive, (D)ead
I L L
L
L
L L
L L L
LL L
L
28.2
Effluent Percentage 13
Treatment 2 Oranniemc 1 7 Z d c A 'r o n rn 11 1.9
Number of Young Produced
132
29T26
0.0
128 128
27
29
27
29
27
26
30
28.2
Adult Survival: (L)ive, (D)ead
I LLL.
0
I L
I L I L
L
L
L
L
L
L
L
0.0
I KWacuo.
Effluent Percentage=
reatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean
lumber of Young Produced
Ault Survival: (L)ive, (D)ead
oa Rcducti,
Effluent Percentage=
rcatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 hlaan
lumber of Young Produced
Ault Survival: (L)ive, (D)ead
Effluent Percentage=
reatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 M.n
lumber of Young Produced
tdult Survival: (L)ive, (D)ead
% Rcductic
Effluent Percentage=
Ireatment6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 MG
lumber of Young Produced
,dult Survival• (L)ive, (D)ead
Rcductic
Overall Analysis:
Result: PASS LOEC: >13% NOEC: 13% ChV: >13%
DWO form AT -3 (8191) Rev. 11195
Chronic Test Results
Reproduction LO EC:
Final Control Mortality (%):
0.0
% Control with 3rd Broods:
100
Control Reproduction CV:
4.5
48 Hour Mortality
Method`.
Control:
0
of
12
IWC:
0
of
12
Significant?-.
No
Final Mortality Significant at: No concentration
Reproduction Analyses
Reproduction LO EC:
>13%
Reproduction NOEC:
13%
Overall Method:
Homoscedastic t
Normal Distribution:
Yes
Method`.
Shapiro-Wilk's
Statistic:
0:933
Critical Value:
0.884
Equal Variances:
Yes
Method:
F -Test
Statistic:
1.906
Critical Vallee:
5.320
Non -Parametric Analysis,017applicable)
Mcthod:
Effluent % Rank Sum
Critical Stan--
um13%
13%
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia Page 1 of 1
(EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Control Bench Sheet
_
Test Grouping Information: Test Organism Informati
1
Daily Renewal Information:
oControl #: �Date: 09-07-16
n.
Organism Source:
(culture board):
-house Culture
24 -hours old
Culture board cup # 4.
Date and time organisms were 00`-T%Llo 0100 r0 two
born between:
Average transfer volume: 0.03 mL
Transfer bowl information: IPH (S.U.): "I -
(°c)` -Lt.1
Day
Facility
Project #
6
MVV%-jtl.Le
lX"1Ito
5
t.1 i la
l
4
c�IF�SIUe
ICI -1
Time Analyst
Initiation/Feeding
m%0d0.
Feeding
Renewal
Feeding3
C
Fg0Ot{Ol2/FedingeA
Rw12/Fe
e J6
Temin(on7
o
0
1
2
4
5
09-07-16
09-08-16
09-09-16
09-10-16qJ�Feeding
09-11-16
09-12-16
09-13-16Al
09-14-16
P-2
Batch
8'�0 A9
�-
Batch
oc IF It,
r
ontrol
Template
gl.LJr~
$
Daily Renewal Information:
oControl #: �Date: 09-07-16
n.
Organism Source:
(culture board):
-house Culture
24 -hours old
Culture board cup # 4.
Date and time organisms were 00`-T%Llo 0100 r0 two
born between:
Average transfer volume: 0.03 mL
Transfer bowl information: IPH (S.U.): "I -
(°c)` -Lt.1
Day
Date
Test Initiation, renewal, feeding or
term nation
SSW Bates
7, 91
6y
4
Dissolved oxygen (mg/L)
Selenastrum
YWT
Location
Randomizing
Time Analyst
Initiation/Feeding
m%0d0.
Feeding
Renewal
Feeding3
C
Fg0Ot{Ol2/FedingeA
Rw12/Fe
e J6
Temin(on7
o
0
1
2
4
5
09-07-16
09-08-16
09-09-16
09-10-16qJ�Feeding
09-11-16
09-12-16
09-13-16Al
09-14-16
C&16
0% Ac,
4- - NI? b
r
Batch
8'�0 A9
�-
Batch
oc IF It,
r
Incubator/Shelf
Z�\
'a`
-T
Template
gl.LJr~
$
Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analvst niti Ar I I_._
Day
PH (S.U.)
Replicate number .
7.63
7, 91
6y
4
Dissolved oxygen (mg/L)
'J q
7
8
7 f 7,
Control, SSW��4
Conductivity (µmhos/cm)(�
12
2
Renewal One
a
rrsb
�,, m
L_
Alkalinity (mg/LCaCO)
`
z t,
I`,
ti-
L
`Hardness (mg/L CaCO,)
L
I I--
L_
5
Renewal Two
`Analyst
Temperature (°C)
-114�ty
��- �
0% 1�• �
1�- � V •�
-
identified far each day, performed pH, dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation, renewal or termination by
the analyst identified in the Daily Renewal information table. Alkalinity and hardness performed by the analysts indentified
L-
IS
on the test bench sheets
and transcribed to this bench sheet.
Survival and Reproduction Data -
-L
J
L,
Day
Observations
Replicate number .
1
2
3
4
5
6
7
8
9
10
1 11
12
2
Renewal One
Adult mortality
(L = Uve, D = dead)
L_
�--
`
I L-
I`,
L
L
E
L
I I--
L_
5
Renewal Two
Number of broods present
Number of young produced
Adult mortality
(L=Live,D=dead)
"t-
Ila
1
1,-`
IS
t
I',
`
LL,
14
Ila
L-
IS
-L
J
L,
7
Final
Number of broods present
Number of young produced
Total young produced
1
114
10
l
1"L
Zb
\
IZ
%1
1
1
p
U
1
1
Z„ l
1
l�j
Z`ti.
1
Z C is
1
1s
L'1
1
ll
Z'1
1
) 1p
l
IL-,
l�
Z.9
p
Z 1
3D
Final adult mortality
(L = Live, D = dead)
X for 3rd Broods
v
of Male Adults (5 20%)
Y'° Adults having 3rd Broods (>_ 80%) O�
% Mortality (5 20%) �-
Mean Offspring/Female
(_> 15 offspring/surviving female)
/�
26Z
% CV (< 40%)
If S?.
SOP AT12 - Exhibit AT12.3, revision 11-01-14
I
��-----4 Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia
(EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Test Bench Sheet
Paired with Control # I Date: OJ -01,16
Client Earth Environmental Services NPDES # NCO020800
Facility Andrews WWTP outfall 001
Project # 1.010 County Cherokee
Test Concentration (Chronic Limit) 13.0% Dilution
preparation:
Samples were not aerated or treated unless otherwise noted on this form. Control, dilution water
and test renewal information are included on the Control Bench Sheet indicated above.
t-rrerrncur rinuryses:
Observations
Initiation
ml
mL
Total volume
Sample
Dilution water
mL
39
261
300
t-rrerrncur rinuryses:
Observations
Initiation
Renewal One
Renewal Two
1
2
Initial
Final
Initial
Final
Initial Final
Concentration
Analyst
10
it
/V6
Renewal One
Mf `,r
pH(S.U.)
�v-7tr
7•fO
7,6 1
7 —f- 63
Test
Dissolved oxygen (mg/L)7
1
7 -7.11
Concentration
Conductivity (µmhos/cm)
W4"y
Z
Z
L
�I
*Temperature (°C)
'L�,
'1$ Q
Z} •
5
Z ,g 15
110
IS
`
(�
1•j_
\'L
100%,
Dissolved oxygen (mg/0
Conductivity (µmhos/cm)
.7
Y
a
�J
-
i�
Renewal Two
Adult mortality
(L = Live, D = dead)
Sap
—r
r .
J s•
-{
`
*Total residual chlorine (mg/L)
C 0.11
- .; 4 0.110
T K -
Sample number
Samples Ib p°10b ab
Sample 2 J600\0if
(
*Analyst identified for each
day. performed DH- dissnived nxvnen —a ..... .:.a
i
the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual
Chlorine Bench Sheet and transcribed to this bench sheet.
Survival and Reproduction Data /narfnrmorf nt toct
Day
Observations
Replicate number
—4--S
2
Adult mortality
1
2
3
from control 11010
6
7
8
9
10
it
12
Renewal One
(L=Live, o=dead)
Number of broods present
Z
Z
Z
Z
L
ZLI1
Z
5
Numberofyoungproduced
110
IS
`
(�
1•j_
\'L
1A
1S
13
is
l(p
Renewal Two
Adult mortality
(L = Live, D = dead)
`
Number of broods present
(
1
1
i
tLLt.
LL
Li
Number of young produced
mo
I�
l ,S
1-2-
16
1 S
IS
1'Z.
1 �0
141
l
1
7
Total young produced
Final
32
7A
1
V- ,
Z&
24
21
IA
�r
G
Final adult mortality
-
(L=Uve,D=dead)
L
ET
L
�
Test was initiated usint
Samole 1. Samole 2 was used fnr R..—.k n—
ram..11
�-a r....,
u,., s[ �_
_ _ ..
___ ,..
, . ,.
pnor to use with sort synthetic water
• -----••--�- •- .— 1—wu�un.auun
and warmed to 25.0 ± 1.0°C in a warm water bath.
Test Results and Statistical Analyses:
. Test results Statistics
% Mortality
•
Mean offspring
Q
�V.2
per female
28
% Reduction
from control 11010
t -Stat or
Rank Sum
t�r�
•�m
1 -Tailed
Critical
28
PASS or FAIL
SOP AT12 - Exhibit AT12.2, revision 11-01-14
0011
Ceriodaphriia duhia Chronic Whole Effluent Toxicity Test
��; I EPA -821-R-02-013, Method 1000.0 - North Carolina Modification
QR
{{ Quality Control
Verification of Data Entry, Calculations and Statistical Analyses
Client: Andrews WWTP
f Environmental Testing Solutions, Inc. Test dares: September 07 - 14, 2016
Project. number: 11720
Reveiwed by: \�a
Concentration
(%)
Day
Dumber of oproduced b replicate number
Survival
(%)
Average.
reproduction
(offspring/female)
v
Coefficient of
variation
M
Percent reduction
from control (%)
1
2
3
4
5
6
7
8
9
10
11
12
Control
5
16
14
15
17
14
16
15
12
16
13
15
17
100
28.2
4.5
Not applicable
7
14
12
12
11
14
13
13
15
11
16
14
13
Total
30
26
27
28
28
29
28
27
27
29
29
1 30
13%
5
16
15
11
16
12
12
14
15
13
13
15
.16
100
28.2
6.2
0.0
7
16
14
15
12
16
15
15
12
16
14
11
14
Total
32
29
26
28
28
27T
29
27
29
27
26
30
Dunnett's MSD value: 1.564 MSD = Minimum Significant Difference
PMSD: 5.6 PMSD = Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the,control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10°i percentile) = 13%.
Upper PMSD bound determined by USEPA (90°i percentile) = 47%.
Lower and upper PMSD bounds were determined from the I Oth and 90th percentile, respectively, of PMSD data from EPA's WET
hnterlaboratory Variability Study (USEPA, 2001a; USEPA, 2001 b).
USEPA. 2001a, 20016. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 -Appendix. EPA -821-13-01-004 and EPA -821-131-01-005.
US Environmental Protection Agency; Cincinnati, 01-1.
Andrvtrs 09-07-16
Ll
roi 28.167 1.0000 28.167 26.000 30.000 4.499 12
13 28.167 1.0000 28.167 26.000 32.000 6.211 12 0.000 2.508 1.564
AuxitiaryTests r_-= ;, Statistic P.Y;Cr�t�cal- Skew Kurt
Shapiro-Wiilk's Test indicates normal distribution (p > 0.01) 0.93258 0.884 0.54371 0.305
F -Test indicates equal variances (p = 0.30) 1.90566 5.31963
HomoscedasfictTest indicates nosignificant differences 1.56421 0.05553 0 2.33333 1 1,22
Treatments vs D -Control
Andrews -09-07-16
z
ap
RE -z
_ Environmental Testing Solutions, Inc.
Ceriodaphnia_Surviv_al and Re_ production Tesf=Reproduction T_ -
Start Date:
9/7/2016
Test ID: CdPFCRNC
Sample ID:
Andrew VWVTP
End Date:
9/142016
Lab ID: ETS-Envir. Tesfing Sol.
Sample Type:
DMR -Discharge Monitoring Report
Sample Date:
Protocol: FWCHR-EPA-821-R-02-013
Test Species:
CD-Ceriodaphnia dubia
Comments:
n
10•
D -Control
30.000
26.000 27.000 28.000 28.000 29.000
28.000 27.000
27.000 29.000
D -Control
29.000
30.000
13
32.000
29.000 26.000 28.0.00 28.000 27.000
29.000 27.000
29.000 27.000
13
26.000
30.000
roi 28.167 1.0000 28.167 26.000 30.000 4.499 12
13 28.167 1.0000 28.167 26.000 32.000 6.211 12 0.000 2.508 1.564
AuxitiaryTests r_-= ;, Statistic P.Y;Cr�t�cal- Skew Kurt
Shapiro-Wiilk's Test indicates normal distribution (p > 0.01) 0.93258 0.884 0.54371 0.305
F -Test indicates equal variances (p = 0.30) 1.90566 5.31963
HomoscedasfictTest indicates nosignificant differences 1.56421 0.05553 0 2.33333 1 1,22
Treatments vs D -Control
Andrews -09-07-16
Whole Effluent Toxid
Chain -of -Custody Form
351 Depot Street
Asheville, NC 28801
Phone: (828) 350-9364
Fax: (828) 350-9368
Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 13%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector). '
Composite sample: Sample location-L,A,11 L= vt- L/ 11ef
Start date: S ( Time: C � � � Volume collected for testing:
End date: Time: D, 0 t_ Number of containers filled for testing: 1 t
Number of samples per hour: - 's, id J,__ � _ Method of transport to laboratory:
Chilled during collection?��' Comments:
If chilled, specify temperature: j G
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample- collector and facility personnel)
Sample collected by:
L-ZA
Print Signature Dal. and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Ott Received at ETS by:
FP14 --O_ W�-1/60 -DIG-110
0111,
Prim Signature Date and time Print
Custody seals intact?:
Yes No Not used
Samples received in good condition?:
Yes No
Tracking number: V�-
Comments:
o�g�mwu uaic into time
Sample temperature upon receipt at ETS (°C):
ySC.
Total residual chlorine upon receipt at ETS: = =
(DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Abscm
Project number: 1(22it7 Sample number: 110 041010t0l0
WIN
4
�r
1
"
ztl
Environmental Testing Solutions, Inc.
Whole Effluent Toxid
Chain -of -Custody Form
351 Depot Street
Asheville, NC 28801
Phone: (828) 350-9364
Fax: (828) 350-9368
Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 13%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector). '
Composite sample: Sample location-L,A,11 L= vt- L/ 11ef
Start date: S ( Time: C � � � Volume collected for testing:
End date: Time: D, 0 t_ Number of containers filled for testing: 1 t
Number of samples per hour: - 's, id J,__ � _ Method of transport to laboratory:
Chilled during collection?��' Comments:
If chilled, specify temperature: j G
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample- collector and facility personnel)
Sample collected by:
L-ZA
Print Signature Dal. and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Ott Received at ETS by:
FP14 --O_ W�-1/60 -DIG-110
0111,
Prim Signature Date and time Print
Custody seals intact?:
Yes No Not used
Samples received in good condition?:
Yes No
Tracking number: V�-
Comments:
o�g�mwu uaic into time
Sample temperature upon receipt at ETS (°C):
ySC.
Total residual chlorine upon receipt at ETS: = =
(DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Abscm
Project number: 1(22it7 Sample number: 110 041010t0l0
rte,tat
l
09-0 1�,
B ti
I
351 Depot Street
Print
Relinquished by:
Signature
Asheville, NC 28801
Phone: (828) 350-9364
Print
Relinquished by:
sigaaturc
Fax: (828) 350-9368
;�e
Environmental Testing solutions, Inc.
Print
Whole Effluent Toxicity Chain -of -Custody Form
Date and time
Facility:
Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001
County: Cherokee
Andrews WWTP Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 13%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Composite sample:
Start date: (4o Time:
End date: c! Time:
Number of samples per hour:
Chilled during collection? �(
If chilled, specify temperature:
Triple rinse sample container with sample before filling.
Pack the sample container completely in ice.
Imnle CIIStOdv[ (tn hr. ---1.4-A t... --1— .._n_ _
Sample location: t ;�tl;� '��'C;c r' 'e,, l/ c- 11�
Volume collected for testing: t r -,r
Number of containers filled for testing: 1
Method of transport to laboratory: S
Comments:
Completely fill the sample container with no air space.
The sample must be < 6.0°C upon receipt at the laboratory.
� .._...r ... .................... .rwur:y Ncia�uuctJ -
Sample collected by:
l
09-0 1�,
/
<Ac
Print
Relinquished by:
Signature
Date and time
Print
Relinquished by:
sigaaturc
Date and lime
Print
Signature
Date and time
aampte receipt mtormatlon: (to be completed by ETS personnel)
Relinquished to ETS by: (1•{.711
1/1'. i C ld-e� j� ft�1 67 b
Print signature Dam and it..
Custody seals intact?: ED
Yes No Not used
Samples received in good condition?:
Yes No
Tracking number: lj {+N
Comments:
Received by:
Print Signature Date and time
Received by:
Print Siymatun: Dat. and time
Received at ETS by:
, gnam. Date and time
Sample temperature upon receipt at ETS (°C):
Total residual chlorine upon receipt at ETS:
(DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Ab=t
Project number:WTU Sample number: V140 b,c-A-
09-0 1�,
, gnam. Date and time
Sample temperature upon receipt at ETS (°C):
Total residual chlorine upon receipt at ETS:
(DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Ab=t
Project number:WTU Sample number: V140 b,c-A-
1.14
1.12
1.10
1.0'8
1.06
1.04
1.02
2.5
2.0
z
1.5
in
N
U 1.0
0.5
1.4
1.3
1.2
1.1
1.0
0.9
0.8
Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
USEPA Control Limits (± 2 Standard Deviations)
USEPA Warning and Control Limits (75th and 90t1i Percentile CVs)
Laboratory Warning and Control Limits (10th and 25th Percentile CVs)
...............................................................................................
oa ��..p5 p5..o6 p9no1 ,p,..09 �A•.09 l? ,�o �b•%,� , ,1Z ,�> >y `Oti p9,pLA9 `p3 ��,oa �5 `oA �5 `oS ��,pb �� ,p1 ,10 �9 -%-10 :%6
Test date
0 7 -day IC,5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride
that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
— — Central Tendency (mean IC25)
— — - Warning Limits (mean IC 25 t SA 10 or SMJ)
......... Control Limits (mean IC 2_ A25 f S , SA.90 , 1
or 2 Standard Deviations) mSumnN
�
"'N�
n
M,
f s x'i
S
g
_O
Environmental Testing Solutions, Inc.
1.14
1.12
1.10
1.0'8
1.06
1.04
1.02
2.5
2.0
z
1.5
in
N
U 1.0
0.5
1.4
1.3
1.2
1.1
1.0
0.9
0.8
Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
USEPA Control Limits (± 2 Standard Deviations)
USEPA Warning and Control Limits (75th and 90t1i Percentile CVs)
Laboratory Warning and Control Limits (10th and 25th Percentile CVs)
...............................................................................................
oa ��..p5 p5..o6 p9no1 ,p,..09 �A•.09 l? ,�o �b•%,� , ,1Z ,�> >y `Oti p9,pLA9 `p3 ��,oa �5 `oA �5 `oS ��,pb �� ,p1 ,10 �9 -%-10 :%6
Test date
0 7 -day IC,5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride
that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
— — Central Tendency (mean IC25)
— — - Warning Limits (mean IC 25 t SA 10 or SMJ)
......... Control Limits (mean IC 2_ A25 f S , SA.90 , 1
or 2 Standard Deviations) mSumnN
�
eM� €
I t j }
EnvironmentalTesting Solutions, Inc.
January 03, 2017
Mr. Michael Ladd
Earth Environmental Services
75 Bison Lane
Murphy, NC 28906
RE: ETS PROJECT NUMBER: 11962
Dear Mr. Ladd:
PO Box 7565
Asheville, NC 28802
Phone: (828) 350-9364
Fax: (828)350-9368
Enclosed are toxicity test results for samples from the Andrews WWTP received by
Environmental Testing Solutions, Inc. December 06 through December 08, 2016.
Parameter Test Procedure EPA Method Final
Code
Number Result
North Carolina Ceriodaphnia Chronic Effluent
TGP3B Toxicity Procedure EPA -821-R-02-013 PASS
(Ceriodaphnia Pass/Fail Toxicity Test)
If this test was performed as an NPDES requirement or by Administrative Letter, please enter a
P on the Effluent Discharge Monitoring Form (MR -1') for the collection date
December 06, 2016 using the parameter code TGP3B.
Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT -3) by
January 31, 2017.
If you have any questions concerning these results, please feel free to contact me.
Sincerely,
Jim umner
Laboratory Director
This report should not be reproduced, except in its entirety, without the written consent of Environmental Testing Solutions, Inc.
The results in this report relate only to the samples submitted for analysis.
North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
® t.
®
Environmental Testing Solutions, Inc.
Effluent Aquatic Toxicitv Report Form - Phase II Chronic Ceriodaphnia dubia
PO Box 7565
Asheville, NC 28802
Phone: (828) 350-9364
Fax: (828)350-9368
Date: January 03, 2017
Facility: Earth Environmental Services NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee
Andrews WWTP
Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: Project: 11962
Samples: 161206.16, 161208.18
Mail Original To: North Carolina Department of Enviromnent and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Set -vice Center Start date: End date: Start time: End time:
Raleigh, NC 27699-1621 12-07-16 12-14-16 1230 0700
Sample Information
Collection start date:
Grab:
Composite duration:
Alkalinity (mg/L CaCO3):
Hardness (mg(L CaCO3):
Conductivity (µmhos/cm):
Total residual chlorine (mg/L):
Sample Temp. at Receipt (°C):
Sample 1
Sample 2
Control
12-05-16
12-07-16
R=.al2
13%
13%
13%
24-h
24-h
Control
7.27
7.47
32, 31, 31
7.22
7.52
40, 40, 42
313
323
155, 158, 165
<0.10
<0.10
7.28
7.8
7.7
7.7
Test Information
Treatment:
Initial pH (SU):
Final pH (SU):
Initial DO (mg/L):
Final DO (ing/L):
Initial Temp. (°C):
Final Temp. (°C):
Organism Number
Control OrEanisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan
Stan
Rcnc-1 l
Rc=,,al2
Stan
R-,,alI
R=.al2
13%
13%
13%
Control
Control
Control
7.27
7.47
7.41
7.22
7.52
7.54
7.53
7.41
7.30
7.47
7.42
7.28
7.8
7.7
7.7
7.8
7.8
7.9
7.9
7.8
7.8
7.9
7.8
7.8
24.8
24.8
24.7
24.8
24.6
24.7
25.1
24.8
25.1
24.9
25.1
25?
Number of Young Produced
27
130
125
126
128
129 129
126
127
130
27
29
L
Adult Survival: (L)ive, (D)ead
L
L
L
L
L
L L
L
L
L
L
L
c c.o
Effluent Percentage13 %
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean
Number of Young Produced
128
129
26 28
25
29
31
32
28
28 32
32 29.0
Adult Survival: (L)ive, (D)ead
L
L
L L
L
L
L
L
L
L L
L1 -4.5
/ Keductlon
Effluent Percentage=
reatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan
lumber of Young Produced
Ldult Survival: (L)ive, (D)ead
%. Redncti,
Effluent Percentage=
-reatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan
lumber of Young Produced
tdult Survival: (L)ive, (D)ead
%. KMW...
Effluent Percentage=
'reatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mcan
lumber of Young Produced
Ault Survival: (L)ive, (D)ead
% Rednciic
Effluent Percentage=
reatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mmn
lumber of Young Produced
.dolt Survival: (L)ive, (D)ead
% Redaaic
Overall Analysis:
Result: PASS LOEC: >13% NOEC: 13% ChV: >13%
DIf,'Q form AT -3 (8/91) Rev. 11195
Chronic Test Results
Reproduction LOEC:
Final Control Mortality (%):
0.0
%Control with 3rd Broods:
100
Control Reproduction CV:
6.0
48 Hour Mortality
Method:
Control: 0
of
12
IWC: 0
of
12
Significant?:
No
Final Mortality Significant at: No concentration
Reproduction Analyses
Reproduction LOEC:
>13%
Reproduction NOEC:
13%
Overall Method:
Homoscedastic t
Normal Distribution:
Yes
Method:
Shapiro-Wilk's
Statistic:
0.952
Critical Value:
0.884
Equal Variances:
Yes
Method:
F -Test
Statistic:
1.983
Critical Value:
5.320
Non -Parametric Analysis (if applicable)
Method:
Effluent % Rank Sum
Critical Sum
13%
Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia
(EPA -821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Control Bench Sheet
Control #: �_ _ Date: 12-07-16
Test Groupinq Information. Test Organism Information:
Facility
Project #
6
< 24 -hours old
5 S
tl�ib
4 1 F -SVI.N
11°t
3
%RIO
2 f%0t.A %0S
t l �--
1- Controlf._,
0.03 mL
Daily Renewal Information:
Organism Source:
In-house Culture
Age:
< 24 -hours old
Source (culture board):
A
Replicate #
1 2 3 4 5 6 1718 9 10 11 12
Culture board cup #
L (. 1118 1 IS 11 1111151203 It,
Date and time organisms were
born between:
-M ►'1-4-C)
Ave'ra'ge transfer volume:
0.03 mL
Transfer bowl information:
pH (S.U.):
Temperature (°C): '�,�•9
Chemical Analyses: Initiation Renewal One
Initial Final Initial Final
Concentration
(s.U.) •'11.. i
Renewal Two
Initial Final
Il. 1 WG
Dissolved oxygen (mg/L)
Test Initiation, renewal, feeding or
71
Selenastrum
YWT
Location
Randomizing .
Day
Date
termination
SSW Batch
(SV
,� �:s
(COj
-
Control, SSW
*Alkalinity (mg/L CaCO3)
Adult mortality
Batch
Batch
Incubator/Shelf
Template
Time Analyst
0
12-07-16
Initiation/Feeding
IN cK
r a
h.,
It'1�•11a
1\.•t,�-ala
'j,-�Z
����..
(L = Live, D = dead)
*Temperature (°C)
Feed113a•i1,8
,T=•
-i. •�
l.`1•�
*�
-i�-1
�'L5 • Z
1
12-08-16
()!kty
performed by the analysts indentiFed on the test bench sheets and transcribed
to this bench sheet.
'_-
2
12-09-16
Renewal JJFeeding
3D to
r ro h
`
+^
r
+ rt w'�* tsC
t EMU it
Z
114%
11
Number of broods present
r
n
Feeding
3
12-10-16
Number of young produced
I Z .
15
`b
I`2..
IZ'
4
12-11-16
Feeding
Q
l Z
k
1(n
r t
t r
Adult mortality
ps
1a e;}
\,—
i
L
L
Renewal2/Feeding
I--
k sYF
r
a
5
12-12-16
x.._
Feeding
1
6
12-13-16
1
I
1
I
1
I
LIL
12-14-16
Termination U-
L
7
Chemical Analyses: Initiation Renewal One
Initial Final Initial Final
Concentration
(s.U.) •'11.. i
Renewal Two
Initial Final
Il. 1 WG
Control Acceptance Criteria:
% of Male Adults (<_ 20%)
Dissolved oxygen (mg/L)
-I A
71
fle
't-o_
4
/IV I
6
Conductivity (µmhos/cm)
l55
.,tea ;y
(SV
,� �:s
(COj
Day
Control, SSW
*Alkalinity (mg/L CaCO3)
Adult mortality
aku
��, t Fra:
3
�E3ac
*Hardness ( g m /LCaCO3)
IN cK
r a
h.,
5
2
(L = Live, D = dead)
*Temperature (°C)
�}`. �'
,T=•
-i. •�
l.`1•�
*�
-i�-1
�'L5 • Z
-Analyst identified for each day, performed pH, dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test
initiation, renewal or termination by
the analyst identified in the Daily Renewal Information table. Alkalinity and hardness
performed by the analysts indentiFed on the test bench sheets and transcribed
to this bench sheet.
Control Acceptance Criteria:
% of Male Adults (<_ 20%)
Observations
Replicate number
1
2 1
3
4
5
6
7
8
9
30
11•
12
Day
2
Adult mortality
L
L
Renewal One
(L = Live, D = dead)
'_-
2
Z`
Z
Number of broods present
5
Number of young produced
I Z .
15
`b
I`2..
IZ'
1
l 10
1�
l Z
I
1(n
1 V`
Renewal Two
Adult mortality
X_
\,—
L
L
tv„
I--
"—
(L = Live, D =dead)
1
t
1
1
I
1
I
1
I
LIL
Number of broods present
L
�5
IS
12-
l J
l �j
13
15
11
1
i 5q
Number of young produced
7
Total young produced
�.�
,LS
Zb
Ze,
ZC`
Zto
wi
-n
Z
Final
Final adult mortality
L
t✓
L
(L= Live, D = dead)
X for 3rd Broods
Control Acceptance Criteria:
% of Male Adults (<_ 20%)
M.
%Adults having 3rd Broods (>_ 80%)
too.
• Mortality (<_ 20%)
Mean offspring/Female
(-e 15 offspring/surviving female)
L t 8
CV (< 40%)
(p,07.
SOP AT12 - Exhibit AT12.3, revision 11-01-14
�-v Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia
(EPA -821-R-02-013 Method 1002.0, NC Modification - December 2010, Version 3.0) - Test Bench Sheet
Paired with Control # I Date: I—L, TI'lik,
Client Earth Environmental Services NPDES # NCO020800
Facility Andrews WWTP outfall 001.
Project # I County
Test Concentration (Chronic Limit) 13.0% Dilution
preparation:
Samples were not aerated or treated unless otherwise noted on this form. Control, dilution water
and test -renewal information are included on the Control Bench Sheet indicated above.
Cherokee
mL
Sample
mL
Dilution water
Totalvolume
mL
39
261
300
Chemical Analyses:
Initiation
Initial Final
Renewal One Renewal Two
Initial Final Initial Final
Concentration
Analyst
HS
4/6
46
t-- 0%-/ NG
Test
Concentration
PH (S.U.)
—4•11
7,j3
7. Y7
/1-41 a.4i 7.30
Dissolved oxygen (mg/L) 7.8 '71 7.7
7
Conductivity (µmhos/cm) /gp X i' 1 a
*Temperature (°C)
�� •S
-A. 8
100%
pH (S. U.)
8 y y k ;
:7.2 .�,� ,�
� �H . �.
%. SD 4x �s WOW
Dissolved oxygen (mg/L) 7.1 7
•�
Conductivity (µmhos/cm) 3/3
*Total residual chlorine (mg/L) 4 p .l, D Spa 10 a 3,!
U
Sample number Sample 1 Il I-Lo1o.16 sample 2 16li-LO. I$
-r muysr taennnea for eacn aay, pertormea prt, atssotvea oxygen ana conauctivtty measurements omy. i emperamres pertormea at ttie time of test initiation, renewal or termination by
the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual
Chlorine Bench Sheet and transcribed to this bench sheet.
Survival and Reproduction Data (performed at test concentration) :
Day
Observations
Replicate number
1
2
3
. 4
5
6
7
8
9
10
it
12
2
Adult mortality
Renewal One
(L = Live, D = dead)
Number of broods present
2
•L
5
Number of young produced
IS
I�
13
��
1-
IS
IS
L -L
IS
1-1
l
RenewalTwo
Adult mortality
(L = Live, D = dead)
Number of broods present
%L
I
LL
1
t
1
1
L
1
Number of young produced
1
I^2..{�j
loFinal
Total young produced
Z%
-46Z-O
t—V-532EI
ZA
3Z
It
—L&
3,Z37-
Final adult mortality
(L= Live, D = dead)
, cx was 1111 ua I.— usmg 3,11 [1 a. -m npre < was Us ror nenewals une tuay [J ana iwo tuay �). dam pies were iniurea to the test concentration prior to use witn sott synthetic water
and warmed to 25.0 ± 1.0°C in a warm water bath.
Comments:
Test Results and Statistical Analyses:
Test results Statistics
%Mortality
1 •5�2
Mean offspring
2R.o
per female
z•
% Reduction
"�•�7
from control
ILi
t -Stat or
Rank Sum
1 •5�2
1-Tailed�j�
z•
Critical
-M/
PASS or FAIL
PASS
SOP AT12 - Exhibit AT12.2, revision 11-01-14
-
�.. r t YS
,-7 f ...P .i
;i Environmental Testing Solutions, Inc.
Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test
EPA -821-R-02-013, Method 1000.0 - North Carolina Modification
Quality Control
Verification of Data Entry, Calculations, and Statistical Analyses
Client:
Test dates:
Andrews WWTP
December 07- 14, 2016
Project number: 11962
Reveiwed by: N..
Concentration
(%)
Day
Number of o ng produced by re licate number
Survival
('%,)
Average
reproduction
(offspring/female)
Coefficient of
variation
(%.)
Percent reduction
from control (1/4)
1
2
3
4
5
6
7
8
9
10
11
12
Control
5
12
15
13
12
12
14
16
13
12
13
16
14
1 100
27.8
6.0
Not applicable
7
15
15
12
14
16
15
13
13
15
17
11
15
Total
27
30
25
26
28
29
29
26
27
30
27
29
13%
5
14
15
14
13
13
17
15
15
12
15
17
18
100
29.0
8.1
-4.5
7
14
14
12
15
12
12
16
17
16
13
15
14
Total
28
29
26
28
25
29
31
32
28
28
32
32
Dunnett's A1SD value: 2.074 MSD = MinitnU n Significant Difference
PIVISD: 7.5 PMSD = Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound detennined by USEPA (10`x' percentile) = 13%.
Upper PMSD bound determined by USEPA (90`x' percentile) = 47%.
Lower and upper PMSD bounds were detennined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET
Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001 b).
USEPA. 2001 a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short -tens Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2 -Appendix. EPA -821-B-01-004 and EPA -821-13-01-005.
US Environmental Protection Agency, Cincinnati, OH.
Andrews 12-07-16
4 1 0 JO
i
TrarisformrUntransformed
:'-Critical
>, 1 Tailed
Cone% Mean _N Mean Mean;
Min ..'Max
.: CU%_
r
D -Control 27.750 1.0000 27.750
25.000
30.000 5.976 12
1.98347
13 29.000 1.0450 29.000
25.000
32.000 8.053 12
'v
MSDu:-
MSDp., ".MSB" „-=E
,- df -
HomoscedastictTestindicatesnosignificantdifferences
2.07405
0.07474 9.375 4.10227
0.14484
1,22
Treatments vs D -Control
` Environmental Testing Solutions,
Inc.
-CeriodaphniaSurvivaland:ReproductionTest-Reproduction-_ , :, ='•,' j
Start Date:
12/7/2016
Test ID:
CdPFCRNC
Sample ID:
Andrews VWVfP
End Date:
12/14/2016
Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR -Discharge Monitoring Report
Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013
Test Species:
CD-Ceriodaphnia dubia
Comments:
Cone"/°
1
.Z, ;: 3;'
4:' ..- `5 6
7 ... _.$-_
``9 10 i
D -Control
27.000
30.000 25.000
26.000 28.000 29.000
29.000 26.000
27.000 30.000
D -Control
27.000
29.000
13
28.000
29.000 26.000
28.000 25.000 29.000
31.000 32.000
28.000 28.000
13
32.000
32.000
i
TrarisformrUntransformed
:'-Critical
>, 1 Tailed
Cone% Mean _N Mean Mean;
Min ..'Max
.: CU%_
t Stat .Critical 'MSD
D -Control 27.750 1.0000 27.750
25.000
30.000 5.976 12
1.98347
13 29.000 1.0450 29.000
25.000
32.000 8.053 12
-1.512 2.508 2.074
?Auxiliary Tests,'• , -,
Statistic'
:'-Critical
-;Skew',
Kurt =
Shapiro-Wilk's Test indicates normal distribution (p > 0.01)
0.952
0.884
-0.0618
-0.7378
F -Test indicates equal variances (p = 0.27)
1.98347
5.31963
,Ii ypothesisiTest,(1 ,tail; 0'.01) , '.•
MSDu:-
MSDp., ".MSB" „-=E
,- df -
HomoscedastictTestindicatesnosignificantdifferences
2.07405
0.07474 9.375 4.10227
0.14484
1,22
Treatments vs D -Control
Andreins 12-07-16
351 Depot Street
Asheville, NC 28801
Phone: (828) 350-9364
Fax: (828) 350-9368
Whole Effluent Toxicity Chain -of -Custody Form
Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: 'Cherokee
Andrews WWTP Purchase order:
Species: Ceriodapitnia dubia Effluent dilution: 13% -
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Composite sample: r Sample location: -till 1-WT—1e11 ao�
Start date: Time: 0� Volume collected for testing: t!
End date: Time: "QC Number of containers filled for testing: j
Number of samples per hour: l,�J. Method of transport to laboratory: ES
Chilled during collection? Y e s Comments:
If chilled, specify temperature:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.0aC upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
S/aim/pplee collected by:
gli
13
7Z�
73Print
Dale and time
Signature
Relinquished by:
Received by:
Environmental
Testing Solutions, Inc.
351 Depot Street
Asheville, NC 28801
Phone: (828) 350-9364
Fax: (828) 350-9368
Whole Effluent Toxicity Chain -of -Custody Form
Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: 'Cherokee
Andrews WWTP Purchase order:
Species: Ceriodapitnia dubia Effluent dilution: 13% -
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Composite sample: r Sample location: -till 1-WT—1e11 ao�
Start date: Time: 0� Volume collected for testing: t!
End date: Time: "QC Number of containers filled for testing: j
Number of samples per hour: l,�J. Method of transport to laboratory: ES
Chilled during collection? Y e s Comments:
If chilled, specify temperature:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.0aC upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
S/aim/pplee collected by:
13
73Print
Dale and time
Signature
Relinquished by:
Received by:
Print
Signature
Dale and lime Print
Signature Dat. and lime
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: 1
Print Signature Date and time
Custody seals intact?: =
Yes No Not used
Samples received in good condition?:
Yes No
Tracking number: (J PC
Comments:
Receivel:tt ETS bv:
rant mgnature uatc ana time
Sample temperature upon receipt at ETS (nC):
Total residual chlorine upon receipt at ETS: =
(DPD Presence/Absence Indicator, MDL = 0.10 mg/L) Present Absent
Project number: I IOltO2 Sample number: 1 �AZC a,L(,0
13
rant mgnature uatc ana time
Sample temperature upon receipt at ETS (nC):
Total residual chlorine upon receipt at ETS: =
(DPD Presence/Absence Indicator, MDL = 0.10 mg/L) Present Absent
Project number: I IOltO2 Sample number: 1 �AZC a,L(,0
,sP
� 351 Depot Street
Asheville, NC 28801
Phone: (828) 350-9364
Fax: (828)350-9368
Environmental Testing Solutions, Inc.
Whole Effluent Toxicity Chain -of -Custody Form
Facility: Earth Environmental Services, Inc. NPDES #: NCO020800 Pipe #: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Cer[odaphnia dub[a Effluent dilution: 13%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Composite sample:
Start date: L �� (%
End date: l �V
Number of samples per hour:
Chilled during collection?
If chilled, specify temperature:
Sample location t O & f F-r—K'i ee' tJ�C►l/�
i /1' -�e�
esting: 1
e
Time: 3:��V Volume collected for testing:
Time: Number of containers filled for t
L S."' 1 $R ia,( Method of transport to laboratory
\j Comments:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
Sample collected by:
Print Signature Date and lime
Relinquished by:
Print Signature Dale and time
Relinquished by:
Print Signature Dale and lima
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: 'aP�
ffi ,.�_
Print Signature Date and lime
Custody seals intact?: = O
Ycs No Not used
Samples received in good condition?: =
Yes No
Tracking number:
Comments:
�44JP
Received by:
Print Signature Dale and time
Received by:
Print Signature Dalc and time
Received at ETS by:
Print Signature Date and lime
Sample temperature upon receipt at ETS (°C):
�r5
Total residual chlorine upon receipt at ETS: = Ei
(DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Present Absent
pl $
Project number: Sample number: J' V IM 119
Quad: Andrews, N.C. N 0 0 0 2 0 8 0 0
Subbasin:040502 Town of Andrews WWTP
Latitude: 35°11'51"
Longitude: 83°50'46"
Receiving Stream: Valle} River
Stream Class: C -Trout HUC#: 06020002
Facility F.
�► r
Location
,1
SCALE 1:24000
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