NEU 2015 Fish Community Templates

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
BUFFALO CR
SR 1941
JF41
04/29/15
Fair
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
JOHNSTON
03020201
35.634275
-78.319049
185
No
Level IV Ecoregion
Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
41.2
1 6
0.5
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
50.5
3.2
no data
37.5
no data
7.6
1.2
0.0
2001
39.2
11.0
1.8
33.9
7.7
6.6
1.4
0.2
2006
38.5
12.2
2.1
32.8
8.2
6.5
1.5
0.4
2011
1 37.9
13.1
1 2.3
1 31.8
1 8.8
1 6.5
1 1.5
1 0.4
Water Quality Parameters
2005
2010
2015
Temperature (°C)
15.2
16.4
14.9
Dissolved Oxygen (mg/L)
8.0
6.7
8.7
Specific Conductance (pS/cm)
53
77
62
pH (s.u.)
6.5
6.0
5.9
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
13
Bottom Substrate (15)
3
Pool Variety (10)
6
Riffle Habitat (16)
3
Bank Erosion (7)
5
Bank Vegetation (7)
6
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5::::]
Total Habitat Score (100)
61
Water Clarity Clear/tannic
Site Photograph
Substrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/29/15
2015-19
15
34
Fair
04/28/10
2010-19
17
44
Good -Fair
04/18/05
2005-26
16
44
Good -Fair
04/05/00
1 2000-06
1 15
1 44
Good -Fair
07/19/95
1 95-67
1 21
1 52
Good
Data
Watershed -- drains eastern Wake and northwest Johnston counties, including the Town of Wendell and the increasingly suburbanized area around Flowers
Crossroads; transitional zone between the Northern Outer Piedmont and the Rolling Coastal Plain; no NPDES permitted dischargers in the watershed; tributary
to Little River. Habitat -- Coastal Plain -like habitats but within the Piedmont; lower to moderate quality habitats (using Piedmont habitat criteria; if using Coastal
Plain habitat criteria score = 74 in 2015), has ranged from 61 to 80 since 2000 (no data were collected in 1995); Coastal Plain -like with extensive wetlands
within the right riparian zone; deadfalls and coarse submerged woody debris; Fissidens. Water Quality -- lowest specific conductance of any site in the Neuse
Piedmont in 2015, has ranged from 53 pS/cm to 77 pS/cm since 2000. 2015 -- more indicative of some Coastal Plain streams with low productivity, few fish
were collected (n = 103), the fewest from any site in the Neuse Piedmont in 2015 and in 2010; a 10-point decline in the NCIBI Score and one NCIBI Rating
class decrease (to a low Fair) were attributed to a slightly lower than expected number of species, the absence of at least two species of sunfish and of any
Intolerant Species, and a high Percentage of Insectivores (92%); only site in the Neuse Piedmont where Dusky Shiner, Tadpole Madtom, and Tessellated
Darter were collected. 2005-2015 -- community shares faunal similarities with some Coastal Plain communities (e.g., Dusky Shiner, Comely Shiner, Eastern
Mudminnow, Tadpole Madtom, Mud Sunfish, Bluespotted Sunfish, and Sawcheek Darter); a very speciose community of 30 species, including 8 species each
of sunfish, 7 species of cyprinids, 5 species of darters, 4 Intolerant Species (Pinewoods Shiner, Sawcheek Darter, Roanoke Darter, and Chainback Darter), but
only 1 species of sucker (Creek Chubsucker); dominant species are Redbreast Sunfish (1995, 2000, and 2005), Dusky Shiner (2000 and 2015), and
Tessellated Darter (2010); the number of fish being collected is consistently low (range 73 - 181), as at other transitional sites, since 2000 the Percentage of
Omnivores+Herbivores has been very low (1 % to 5%) and the Percentage of Insectivores was relatively high (85% to 92%) which resulted in lower than
expected trophic metric scores and overall NCIBI Scores and NCIBI Ratings during these four cycles. Recommendation -- continue basinwide monitoring of
this site in 2020 to document suburbanization of the watershed. This site will be re -sampled in 2016 to verify its 2015 rating.
Most Abundant Comely Shiner (19, 18%) Non -indigenous Imperiled
Species and Dusky Shiner (18, Species None Species None
17 /o )

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
CRABTREE CR
SR 1664
JF24
05/21/15
Excellent
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
35.844708
-78.711774
230
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
84.0
16
1 0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile)
NPDES Number
Volume (MGD)
Town of Cary's North Cary Water Reclamation Facility
NCO048879
12
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
70.4
12.9
no data
11.7
no data
1.9
2.1
0.9
2001
39.8
47.6
14.1
5.9
2.5
1.3
1.9
0.2
2006
34.7
54.1
16.6
4.5
3.2
1.2
1.8
0.4
Water Quality Parameters
2005
2010
2015
Temperature (°C)
21.1
22.2
22.1
Dissolved Oxygen (mg/L)
6.9
7.9
6.5
Specific Conductance (pS/cm)
190
213
265
pH (s.u.)
6.7
7.0
6.6
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
14
Bottom Substrate (15)
8
Pool Variety (10)
10
Riffle Habitat (16)
5
Bank Erosion (7)
6
Bank Vegetation (7)
7
Light Penetration (10)
8
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
73
Water Clarity I Slightly turbid
Site Photograph
Substrate: Cobble, gravel, boulder
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
05/21 /15
2015-31
20
54
Excellent
04/07/10
2010-06
22
54
Excellent
05/13/05
2005-49
26
58
Excellent
06/22/00
2000-46
19
54
Excellent
Data
Watershed -- the largest watershed assessed in the Neuse Piedmont in 2015; drains the extensively urbanized area of northwest Wake and southeast Durham
counties including the cities of Raleigh, Durham, Cary, and Morrisville, and Umstead State Park; one NPDES permitted discharger in the watershed
(NC0048879, located - 7 miles upstream); tributary to Neuse River. Habitats -- moderate quality habitats, has ranged from 73 to 79 since 2000; deep pool at
end of the reach downstream of the bridge; infrequent, shallow cobble and gravel riffles; runs and side snags; Podostemum common. Water Quality --
specific conductance elevated as a result of the upstream W WTP discharge and a highly developed watershed, has ranged from 190 pS/cm to 265 pS/cm
since 2000 with the highest measurement recorded in 2015. 2015 -- a slight decrease in the number of fish collected and the loss of two species of suckers
was offset by the greater percentage of Omnivores+Herbivores such that the NCIBI Score and NCIBI Rating did not change. 2000-2015 -- despite its urbanized
watershed, a diverse community of 29 species are known from this site, including 9 species of sunfish, 8 species of cyprinids, 4 species of suckers, 3 species of
darters, and 2 Intolerant Species (Pinewoods Shiner and Roanoke Darter); dominant species are Swallowtail Shiner (2000), Bluegill and Redbreast Sunfish
(2005), and Fantail Darter (2010 & 2015); site has rated Excellent since 2000 with NCIBI Scores of either 54 or 58. Recommendation -- continue monitoring of
this basinwide site in 2020 to determine any impacts from the WWTP and the continued urbanization of its watershed.
Most Abundant Fantail Darter (26, 12%) Non -indigenous Green Sunfish (7), Bluegill Imperiled None
Species Species (18), and Redear Sunfish (1) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclass ification
DEEP CR
SR 1734
JF3
04/13/15
Good
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
PERSON
03020201
36.240370
-78.888996
400
Yes
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Carolina Slate Belt
32.5
1 10
0.4
Upstream NPDES Dischargers (z 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I ---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
75.5
1.2
no data
22.0
no data
1.0
0.3
0.0
2001
62.4
4.5
0.5
24.4
7.1
1.3
0.3
0.1
2006
60.6
4.5
0.5
24.4
8.9
1.3
0.3
0.1
2011
56.9
4.5
0.5
24.3
12.5
1.3
0.3
0.1
Water Quality Parameters
2000
2005
2015
Temperature (°C)
11.0
14.0
14.2
Dissolved Oxygen (mg/L)
9.8
9.4
9.5
Specific Conductance (pS/cm)
59
63
79
pH (s.u.)
7.2
---
6.1
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
18
Bottom Substrate (15)
10
Pool Variety (10)
10
Riffle Habitat (16)
10
Bank Erosion (7)
5
Bank Vegetation (7)
6
Light Penetration (10)
9
Left Riparian Score (5)
5
Right Riparian Score (5)
4
Total Habitat Score (100)
82
Water Clarity I Slightly turbid
Substrate: Carolina Slate Belt -type rocks, cobble
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/13/15
2015-07
16
52
Good
04/06/05
2005-12
21
58
Excellent
04/06/00
2000-08
22
56
Excellent
05/16/95
95-42
21
56
Excellent
07/19/90
90-06
T 22
60
Excellent
Data
Watershed -- drains southeast Person County; no municipalities or NPDES dischargers in the watershed; tributary to the Flat River and Lake Michie, site is -
0.8 mile upstream from the creek's confluence with the river. Habitat -- high quality habitats, has ranged from 81 to 86 since 2000 (no data were collected in
1990 or 1995); slick rocks covered with periphyton, Fissidens present, and a bluegreen algal film; runs, side snags, and riffles. Water Quality -- low flow;
specific conductance low for an eastern Piedmont stream, ranging from 59 pS/cm to 79 pS/cm since 1990. 2015 -- 1 of only 2 sites (the other being North Flat
River) where four species of darters were present; a 6-point decline in the NCIBI Score and one NCIBI Rating class decrease were attributed to the absence of
one or more species of suckers (White Sucker, Creek Chubsucker, Northern Hog Sucker, V-Lip Redhorse, and Blacktip Jumprock) and the absences of the
intolerant Pinewoods Shiner and the piscivorous Largemouth Bass; however, none of these metric declines are likely indicative of significant changes in water
quality. 1990-2015 -- a very speciose community with 34 species known from the site, including 9 species of cyprinids, 7 species of sunfish, 6 species of
suckers, 4 species of darters, and 3 Intolerant Species (Pinewoods Shiner, Roanoke Darter, and Chainback Darter); dominant species are the Bluehead Chub
and White Shiner; several metrics are extremely stable with every sampling effort represented by 4 species of darters, 4 or 5 species of sunfish, 2 or 3
Intolerant Species, 32% Omnivores+Herbivores, and 70% Insectivores; fish community has been rated Excellent in 4 of the 5 monitoring cycles.
Recommendation -- continue basinwide monitoring of this regional reference site in 2020 to document its support of its supplemental classification as
Outstanding Resource Waters in the face of the potential suburbanization of its watershed.
Most Abundant Bluehead Chub (160, 32% Non -indigenous Green Sunfish (18) and Imperiled None
Species Species 113luegill (7) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
ELLERBE CR
SR 1709
JF4
03/31/15
Poor
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
DURHAM
03020201
36.021971
-78.870404
285
No
Level IV Ecoregion
Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Triassic Basins
10.3
1 7
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
25.4
67.6
no data
4.2
no data
2.3
0.4
0.1
2001
9.6
87.1
23.7
0.7
0.5
1.3
0.2
0.7
2006
9.4
87.1
25.0
0.7
0.6
1.3
0.2
0.7
2011
8.5
88.2
25.9
0.6
0.6
1.3
0.2
0.6
Water Quality Parameters
1995
2005
2015
Temperature (°C)
15.0
14.8
13.1
Dissolved Oxygen (mg/L)
9.1
5.6
13.1
Specific Conductance (pS/cm)
218
167
269
pH (s.u.)
1 6.9
6.7
1 6.8
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
1
Instream Habitat (20)
6
Bottom Substrate (15)
2
Pool Variety (10)
6
Riffle Habitat (16)
0
Bank Erosion (7)
4
Bank Vegetation (7)
5
Light Penetration (10)
7
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
41
Water Clarity I Slightly turbid, dark green
(Substrate: Sand, clay, rip/rap, boulders, bricks
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
03/31/15
2015-06
7
32
Poor
04/14/05
2005-22
9
28
Poor
04/11/95
95-16
7
26
Poor
Data
Watershed -- drains central Durham County, including the City of Durham; greatest percentage of its watershed developed and in impervious cover and least
amount in forest, cultivation, and grassland/herbaceous/shrubland than any other Neuse Piedmont watershed in 2015; no NPDES dischargers in the
watershed; tributary to Falls Reservoir (Neuse River), site is - 6 miles upstream from the reservoir. Habitat -- lowest quality habitats of any Neuse Piedmont or
Coastal Plain stream in 2015, has ranged from 34 to 49 since 1995; deeply entrenched (canyon -like) and channelized; one continuous run of varying depths;
some snags; urban debris throughout the channel; rust -colored iron bacteria along the edges from the groundwater seepage. Water Quality -- second greatest
specific conductance of any site in the Neuse Piedmont in 2015, ranging from 167 pS/cm to 269 pS/cm since 1995 with the greatest measurement recorded in
2015 indicative of stormwater runoff from a highly urbanized and impervious watershed; dissolved oxygen in 2015 was at 125% of saturation. 2015 -- fewest
species (n = 7) collected from any site in the Neuse Piedmont in 2015; darters absent and only one species of sunfish (tolerant Redbreast Sunfish) was
present; a very abundant community, but 97% of all fish were Swallowtail Shiner (87%) and Redbreast Sunfish (10%); two intolerant Pinewoods Shiner were
collected, but they were probably downstream migrants from the Eno or Flat rivers; a very altered trophic structure was present with 99% Insectivores and only
1% Omnivores+Herbivores; lowest NCIBI Score and NCIBI Rating (along with Walnut Creek at SR 2544, Wake County) of any site in the Neuse Piedmont in
2015. 1995-2015 -- number of fish collected is variable with > 600 collected in 1995 and 2015, but only 81 in 2005; only 12 species known from the site,
darters are absent; dominant species is Swallowtail Shiner; low species richness and skewed trophic structure are the norm. Recommendation -- because of
its severely degraded habitat, water quality, and fish community, monitoring needs to be conducted only once every 10 years until more stringent stormwater
controls are implemented throughout the watershed.
Most Abundant Swallowtail Shiner (518, Non -indigenous None Imperiled None
Species 87%) Species Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
ENO R
SR 1336
JF6
04/14/15
Good
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
ORANGE
03020201
36.123333
-79.155556
560
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Carolina Slate Belt
26.7
1 10
1.0
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
63.7
0.3
no data
33.8
no data
0.5
1.7
0.0
2001
51.6
6.1
0.7
34.4
3.4
0.9
2.5
0.1
2006
51.6
6.1
0.7
34.3
4.5
0.9
2.5
0.1
Water Quality Parameters
2005
2010
2015
Temperature (°C)
15.5
15.1
12.5
Dissolved Oxygen (mg/L)
8.1
7.3
9.5
Specific Conductance (pS/cm)
73
73
79
pH (s.u.)
1 6.3
5.8
1 5.9
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
12
Bottom Substrate (15)
2
Pool Variety (10)
10
Riffle Habitat (16)
12
Bank Erosion (7)
3
Bank Vegetation (7)
5
Light Penetration (10)
7
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
66
Water Clarity I Slightly turbid -turbid
Substrate: clay, sand, bedrock, boulder, cobble
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/14/15
2015-10
18
50
Good
04/23/10
2010-13
17
52
Good
04/07/05
2005-15
18
60
Excellent
04/04/00
2000-11
18
54
Excellent
Data
Watershed -- drains northwest Orange County, upstream from the Town of Hillsborough; no NPDES permitted dischargers or municipalities in the watershed;
reach includes the remnants of the historic Halls Mill Dam; flows are regulated by upstream releases from WS-II reservoirs Lake Orange and West Fork
Reservoir; tributary to Corporation Lake, site is - 3.5 miles upstream from the creek's confluence with the lake. Habitats -- moderate quality instream and
riparian habitats, has ranged from 66 to 81 since 2000; slick and eroding clay banks and instream deposits of soft sediments; long, deep pool at the end of the
reach; cobble and boulder rifles at the old mill site. Water Quality -- specific conductance within the norm for an eastern Piedmont stream, relatively stable
since 2000, ranging from 73 pS/cm to 83 pS/cm (in 2000); water temperature in 2015 was colder than that in nearby streams due to the upstream dam
releases. 2015 -- a slight 2-point difference in the NCIBI Score was due to a skewed trophic structure (from 33% in 2010 to 40% in 2015 for
Omnivores+Herbivores and from 84% in 2010 to 60% in 2015 for Insectivores). 2000-2015 -- 27 species known from the site, including 8 species of sunfish, 6
species of cyprinids, 4 species each of darters and suckers, and 3 Intolerant Species (Pinewoods Shiner, Roanoke Darter, and Chainback Darter); dominant
species vary among Swallowtail Shiner (2000) to Bluehead Chub (2005 and 2015) to Redbreast Sunfish (2010); several metrics are stable with every sampling
effort represented by 17 or 18 species, 3 or 4 species of darters, 4 or 6 species of sunfish, and 2 or 3 Intolerant Species; there has been a 10-point decline in
the NCIBI score between 2005 and 2015. Recommendation -- continue basinwide monitoring of this site in 2020 to determine if the decline in the biological
integrity of the community is attributable to altered hydrology, declining water quality, or both factors.
Most Abundant Bluehead Chub (93, 40%) Non -indigenous Green Sunfish (1) and Imperiled None
Species Species Bluegill (2) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
HORSE CR
SR 1923
JF10
04/15/15
Excellent
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
35.978611
-78.561111
280
No
Level IV Ecoregion
Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
12.0
1 8
4.0
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
78.7
1.6
no data
17.2
no data
1.6
0.4
0.5
2001
62.8
15.8
2.2
13.9
5.5
1.6
0.4
0.1
2006
57.8
23.2
3.2
10.8
6.2
1.6
0.4
0.0
2011
55.3
26.2
3.8
10.2
6.3
1.6
0.3
0.0
Water Quality Parameters
2004
2015
Temperature (°C)
---
23.8
17.1
Dissolved Oxygen (mg/L)
---
7.9
9.8
Specific Conductance (pS/cm)
---
64
87
pH (s.u.)
---
6.4
1 6.4
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
10
Bottom Substrate (15)
4
Pool Variety (10)
8
Riffle Habitat (16)
2
Bank Erosion (7)
2
Bank Vegetation (7)
4
Light Penetration (10)
6
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
51
Water Clarity I Slightly turbid
Substrate: Cobble, boulder, sand, red clay, bedrock shelf
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/15/15
2015-14
26
58
Excellent
06/10/04
2004-79
25
52
Good
Data
Watershed -- drains northeast Wake and southwest Franklin counties west of the US 1 corridor; no municipalities or NPDES dischargers in the watershed;
tributary to Falls Lake, site is - 1.5 miles upstream from the reservoir; site is at the old Thompson Mill Dam. Habitat -- lower quality habitats above old mill site,
was scored a "54" in 2004; legacy deposits of soft sediments in the creek; steep, clay banks; channel filled in with sediment above the bedrock shelf. Water
Quality -- specific conductance within the norm for an eastern Piedmont stream, was 64 pS/cm in 2004. 2015 -- a very diverse community, the most species
(n=26) collected at any site in the Neuse Piedmont in 2015, including 5 species of sunfish and 4 species of suckers; almost the same number of fish collected in
2015 as in 2004 (n = 428 and 421, respectively); only metric not scoring a maximum of "5" was the Number of Intolerant Species (n = 1, score = "3" ); a 6 point
increase in the NCIBI Score and one NCIBI Rating class increase were due to the collection of an additional species of darter (Glassy Darter) and an Intolerant
Species (Pinewoods Shiner), and a greater percentage of species with multiple age classes in 2015 than in 2004 (54% vs. 44%, respectively); highest NCIBI
Score and NCIBI Rating (along with South Fork Little River) of any site in the Neuse Piedmont in 2015. 2004 & 2015 -- a very diverse community with 32
species known from the site, including 8 species each of cyprinids and sunfish, 4 species of suckers, and 3 species of darters; high taxa richness may be due in
part to the site's proximity to the reservoir with several species migrating to and from the reservoir (e.g., Yellow Perch, White Perch, Gizzard Shad, Notchlip
Redhorse, V-Lip Redhorse, and Bluegill). Recommendation -- continue monitoring of this site in 2020 to document increasing suburbanization of its
Most Abundant Bluegill (102, 24%) Non -indigenous Green Sunfish (4) and Imperiled None
Species ISpecies Bluegill (102) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
LITTLE CR
SR 1562
JF25
04/28/15
Good -Fair
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
JOHNSTON
03020201
35.575278
-78.443333
150
No
Level IV Ecoregion
Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
17.3
1 7
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
45.3
12.5
no data
37.4
no data
4.4
0.5
0.0
2001
32.0
28.0
6.5
26.5
0.6
3.0
0.5
0.4
2006
28.8
31.7
7.6
25.0
9.5
2.9
0.5
1.6
2011
27.7
34.5
8.6
23.9
9.9
2.8
0.5
0.7
Water Quality Parameters
2005
2010
2015
Temperature (°C)
12.5
15.4
14.3
Dissolved Oxygen (mg/L)
8.3
6.4
9.7
Specific Conductance (pS/cm)
82
88
88
pH (s.u.)
1 6.4
6.0
1 6.1
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
12
Bottom Substrate (15)
3
Pool Variety (10)
10
Riffle Habitat (16)
3
Bank Erosion (7)
4
Bank Vegetation (7)
6
Light Penetration (10)
8
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
61
Water Clarity I Clear/tannic
Substrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/28/15
2015-18
19
44
Good -Fair
04/28/10
2010-18
28
56
Excellent
04/18/05
2005-25
24
46
Good
Data
Watershed -- drains the increasingly urbanized area of western Johnson County and the City of Clayton, headwaters flow along the US 70 corridor; no NPDES
permitted dischargers in the watershed; within a transitional zone between the Northern Outer Piedmont and the Rolling Coastal Plain; tributary to Swift Creek,
site is - 1.3 miles upstream of the stream's confluence with Swift Creek. Habitats -- has ranged from 55 to 69 since 2005; riffles created by sticks in the
current; snags and deadfalls; bank erosion moderate; wide riparian zones comprised primarily of exotic vegetation (Oriental Privet and Japanese
Honeysuckle). Water Quality -- specific conductance within the norm for an eastern Neuse Piedmont stream, stable, and ranging from 82 pS/cm to 88 pS/cm
during the past three monitoring cycles. 2015 -- a 12-point decline in the NCIBI Score and two NCIBI Rating class decrease were attributed to the lower than
expected number of fish (n = 216 vs. 280 in 2010), the loss of one species of sucker (Notchlip Redhorse), and a skewed trophic structure (8%
Omnivores+Herbivores, 92% Insectivores, and 0.5% Piscivores); one male American Shad (470 mm TL) netted and release, the first ever collected by DW R's
fish community monitoring program. 2005-2015 -- community shares faunal similarities with some Coastal Plain communities (e.g., Sea Lamprey, Flier, and
Yellow Perch); a very speciose community of 33 species, including 9 species each of cyprinids and of sunfish, 4 species of darters, 3 species of suckers, and 2
Intolerant Species (Pinewoods Shiner and Roanoke Darter); dominant species are Redbreast Sunfish (2005, 2010), White Shiner (2010), and Johnny
Darter/Tessellated Darter Intergrades (2015); several metrics have been stable (3 species of darters, 2 or 3 species of suckers, and - 25% Tolerant Fish); as at
other ecotonal (transitional) sites, in 2005 and 2015 the Percentage of Omnivores+Herbivores was very low (2% and 8%) and the Percentage of Insectivores
was high (91 % and 92%) which resulted in lower than expected trophic metric scores and overall NCIBI Scores and NCIBI Ratings during those two cycles.
Recommendation -- continue basinwide monitoring of this site in 2020 to document suburbanization of its watershed.
Most Abundant Johnny Darter/Tessellated Non -indigenous Green Sunfish (3) and Imperiled None
Species Darter (55, 25%) Species Bluegill (15) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclass ification
LITTLE R
SR 2224
JF43
05/26/15
Good
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
35.913705
-78.386959
260
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
25.5
1 10
0.4
Upstream NPDES Dischargers (z 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I ---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
51.2
0.0
no data
41.8
no data
4.2
1.8
0.0
2001
37.0
7.5
1.2
39.7
8.3
2.6
2.4
0.0
2006
37.5
7.6
1.2
38.2
11.7
2.6
2.4
0.1
Water Quality Parameters
2004
2010
2015
Temperature (°C)
26.8
15.9
21.3
Dissolved Oxygen (mg/L)
5.3
7.9
5.6
Specific Conductance (pS/cm)
73
67
73
pH (s.u.)
6.3
6.2
6.1
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
12
Bottom Substrate (15)
3
Pool Variety (10)
9
Riffle Habitat (16)
2
Bank Erosion (7)
6
Bank Vegetation (7)
6
Light Penetration (10)
5
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
58
Water Clarity I Slightly turbid
Substrate: Sand, bedrock outcrops
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
05/26/15
2015-34
16
46
Good
04/29/10
2010-22
19
46
Good
06/11 /04
2004-81
20
52
Good
Data
Watershed -- drains northeast Wake and southeast Franklin counties with increasing encroachment of urbanization; greatest percentage of its watershed
under cultivation than any other Neuse Piedmont watershed (along with South Flat River) in 2015; no NPDES permitted dischargers in the watershed; remnants
of Mitchell Millpond are immediately upstream; a unique geological area transitioning between the Northern Outer Piedmont and the Rolling Coastal Plain; part
of a state natural heritage area; tributary to Neuse River. Habitats -- low (below the bridge) to high (above the bridge) quality habitats, has ranged from 58 to
85 since 2004, no change since 2010; Coastal Plain -like habitats; deep, silty bottom pools; deadfalls and coarse woody debris snags; infrequent stick riffles;
infrequent boulder outcrops; open canopy. Water Quality -- second lowest specific conductance of any site in the Piedmont in 2015, has ranged from 67
pS/cm to 73 pS/cm since 2004. 2015 -- no change in the NCIBI Score or NCIBI Rating between 2010 and 2015, but there was a slight decline in the number of
fish (from 252 to 187) and in the species richness of sunfish (from 6 species to 3 species due to the absence of Mud Sunfish, Bluespotted Sunfish, and Green
Sunfish) which was offset by a lower Percentage of Tolerant Fish (from 55% to 35%); as at other transitional sites, the Percentage of Omnivores+Herbivores
was very low (9%) and the Percentage of Insectivores was high (88%), often resulting in an skewed trophic structure; the only Piedmont site where Sawcheek
Darter was collected. 2004-2015 -- community shares strong faunal similarities with Coastal Plain fish communities, 23 species are known from the site,
including 9 species of sunfish, 3 species each of cyprinids and darters, 2 Intolerant Species (Roanoke Darter and Sawcheek Darter), and 1 species of suckers;
dominant species have been Bluegill (2004), Eastern Mosquitofish (2010), and Redbreast Sunfish (2015); number of species and number of fish (from 283 to
252 to 187) appears to be gradually declining, although trophic metrics are stable at - 7% Omnivores+Herbivores and - 88% Insectivores; no change in the
NCIBI Rating since 2004. Recommendation -- continue monitoring this transitional ecoregion and basinwide site in 2020 prior to impoundment of the river by
the proposed downstream Little River Reservoir and to determine any impacts from changes in landuse within its watershed.
Most Abundant Redbreast Sunfish (53, Non -indigenous Imperiled
SSpecies 28% Bluegill (30) None
p ) Species Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
MARKS CR
SR 1714
JF26
04/28/15
Good -Fair
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
JOHNSTON
03020201
35.705965
-78.431554
155
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
26.4
1 8
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse °
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
64.7
1.8
no data
27.6
no data
5.0
0.8
0.0
2 0
53.6
8.5
.4
21.7
12.3
4.1
0.8
0.
22.4
10.7
4.
2
7.
13.7
.8
.2
Water Quality Parameters
2005
2010
2015
Temperature (°C)
18.2
16.7
14.0
Dissolved Oxygen (mg/L)
7.9
7.9
10.1
Specific Conductance (pS/cm)
65
82
74
pH (s.u.)
6.5
6.1
6.0
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
12
Bottom Substrate (15)
3
Pool Variety (10)
6
Riffle Habitat (16)
2
Bank Erosion (7)
5
Bank Vegetation (7)
5
Light Penetration (10)
5
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
53
Water Clarity IVery slightly turbid/tannic
Substrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/28/15
2015-17
21
40
Good -Fair
04/28/10
2010-20
20
48
Good
04/08/05
2005-19
22
54
Excellent
04/05/00
2000-07
18
50
Good
05/18/95
95-46
19
50
Good
09/23/91
91-24
17
46
Good
Data
Watershed -- drains east -central Wake County, below the towns of Knightdale and Wendell in an increasingly suburbanized area; no NPDES permitted
dischargers in the watershed; transitional zone between the Northern Outer Piedmont and the Rolling Coastal Plain; tributary to Neuse River, site is -1.4 miles
above the creek's confluence with the river. Habitats -- Coastal Plain -like but within the Piedmont; low quality habitats in 2015, has ranged from 53 to 82
since1995 (no data were collected in 1991); one bend in the channel; minimal instream and riparian bank habitats; riparian densely vegetated with Oriental
Privet, Cat Brier, and vines; open canopy over the stream; riffles created by sticks in the current; Fissidens. Water Quality -- specific conductance within the
norm for an eastern Neuse Piedmont stream, has ranged from 65 pS/cm to 82 pS/cm since 1995 (data were not collected in 1991). 2015 -- few fish present (n
= 158; 468 were collected in 2010); an 8-point decline in the NCIBI Score and one NCIBI Rating class decrease (to a low Good -Fair) were attributed to the
lower than expected number of fish, the absence of suckers and the intolerant Roanoke Darter, a relatively high Percentage of Tolerant Fish (47%, primarily
Satinfin Shiner and Redbreast Sunfish), and a very low percentage of species with multiple age classes (only 33%; 11 of the 21 species were represented by
only 1 or 2 fish, indicative of a loss of age classes either due to drought or high flows). 1991-2015 -- community shares faunal similarities with some Coastal
Plain communities (e.g., Bluespotted Sunfish, Sea Lamprey, and Comely Shiner), a very speciose community with 32 species, including 9 species of cyprinids,
7 species of sunfish, 4 species of darters, 3 Intolerant Species (Pinewoods Shiner, Roanoke Darter, and Chainback Darter), and 2 species of suckers; dominant
species are Redbreast Sunfish (1991, 2010), Bluehead Chub (1995), Swallowtail Shiner and Tessellated Darter (2000), White Shiner (2005), and Satinfin
Shiner (2015); as at other transitional sites, the Percentage of Omnivores+Herbivores was very low (6%) and the Percentage of Insectivores was high (89%), in
1991 and 1995 the Percentage of Omnivores+Herbivores was (23%) and the Percentage of Insectivores was (69%); since 2005 the NCIBI Score has declined
by 14-points and the NCIBI Rating has declined two rating classes from Excellent to Good -Fair, suggesting a decline in water quality. Recommendation --
continue monitoring of this site to document increasing suburbanization of its watershed.
Green Sunfish (2), Bluegill
Most Abundant Satinfin Shiner 50, 32% Non -indigenous Imperiled
Species ( ) Species (12), Channel Catfish (1), Species None
and Flathead Catfish (1)

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
MIDDLE CR
SR 1375
JF34
05/21/15
Good
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
35.635556
-78.728056
260
No
Level IV Ecoregion
Drainage Area (miZ)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
35.6
10
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile)
NPDES Number
Volume (MGD)
Brighton Forest WWTP
NCO066150
0.117
Amherst WW I F,
NCO061638
0.053
Crooked ree
NCO062715
0.150
City of Cary's South Cary Water Reclamation Facility
NCO065102
16
own ot Apex's Water Reclamation Facility
NCO064050
3.6
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
61.6
8.0
no data
25.1
no data
3.6
1.5
0.2
2001
42.3
29.1
5.6
14.2
9.0
4.4
0.9
0.0
2006
36.2
37.6
7.7
12.0
8.6
4.3
1.1
0.3
2011
1 34.1
1 41.5
1 9.2
1 10.5
1 8.5
1 4.2
1 1.1
1 0.2
Water Quality Parameters
2010
2011
2015
Temperature (°C)
17.5
25.7
22.6
Dissolved Oxygen (mg/L)
6.5
5.5
6.3
Specific Conductance (pS/cm)
250
320
288
pH (s.u.)
1 6.6
6.4
1 6.4
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
14
Bottom Substrate (15)
3
Pool Variety (10)
8
Riffle Habitat (16)
5
Bank Erosion (7)
4
Bank Vegetation (7)
6
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
65
Water Clarity Clear/tannic
Substrate: Sand, gravel
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
05/21 /15
2015-33
20
48
Good
06/02/11
2011-27
21
40
Good -Fair
04/21/10
2010-11
20
44
Good -Fair
07/20/04
2004-131
27
54
Excellent
Data Analysis
Watershed -- drains southeastern Wake County, including the increasingly developed cities of Fuquay-Varina, Cary, and Apex and their suburbs; seven NPDES
facilities upstream discharging a total Q, = 19.96 MGD; transitional zone between the Northern Outer Piedmont and the Rolling Coastal Plain; tributary to the
Neuse River. Habitats -- moderate quality habitats, has ranged from 63 to 78 since 2004; Coastal Plain -like with extensive wetlands and bottomlands within
both of the riparian zones; gravel riffle at upper end of reach; deadfalls and coarse woody debris snags; blow-out pools at bends in the creek; Oriental Privet
growing in the riparian zones. Water Quality -- a detectable chlorine odor from the WWTP effluent; greatest specific conductance of any site in the Neuse
Piedmont in 2015, has ranged from 250 pS/cm to 320 pS/cm since 2004. 2015 -- number of fish collected was very low (n = 145) and the Percentage of
Tolerant Fish was relatively high (50%, primarily Eastern Mosquitofish, Redbreast Sunfish, Satinfin Shiner, and Flat Bullhead); an 8-point increase in the NCIBI
Score and a one NCIBI Rating Class increase resulted from the collection of an additional species of darter (Glassy Darter), a very slight decrease in the
Percentage of Insectivores (from 91 % to 90% between 2011 and 2015), and a greater percentage of species with multiple age classes (65% in 2015 vs. 48% in
2011); as at other transitional sites, the Percentage of Omnivores+Herbivores was very low (7%) and the Percentage of Insectivores was high (90%); 11 large
specimens (320-453 mm TL) of Notchlip Redhorse and V-lip Redhorse were encountered. 2004-2015 -- community shares faunal similarities with Coastal Plain
and Piedmont fish communities; a very speciose community with 32 species known from the site, including 10 species of sunfish, 7 species of cyprinids, 4
species of darters, 3 species of suckers, and 3 Intolerant Species (Chainback Darter which has not been collected since 2004 and Pinewoods Shiner and
Roanoke Darter); dominant species are the tolerant Redbreast Sunfish (2004-2011) and the tolerant Eastern Mosquitofish (2015); a consistently low number of
fish collected (n = 121-231) along with a high percentage of them being Tolerant Fish (34%-50%), and a skewed trophic structure (- 6% Omnivores+Herbivores
and - 90% Insectivores); NCIBI Scores have varied from 40 to 54 with NCIBI Ratings ranging from a low Good -Fair to a low Excellent. Recommendation --
continue monitoring this transitional ecoregion and basinwide site in 2020 to determine any impacts from the WWTP and the continued suburbanization of its
watershed.
Most Abundant Eastern Mosquitofish (26, Non -indigenous Bluegill (7) and Redear Imperiled
Species 118%) Species Sunfish (1) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
MOCCASIN CR
NC 231
JF48
05/27/15
Good
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
J OH NSTON
03020203
35.729049
-78.207221
155
No
Level IV Ecoregion
Drainage Area (mi)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
56.6
9
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile)
NPDES Number
Volume (MGD)
City of Raleigh's Little Creek W WTP
NCO079316
1 2.2
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
58.0
2.5
no data
29.4
no data
8.8
1.4
0.0
2001
46.5
10.2
2.0
30.9
3.5
6.7
1.0
0.1
2006
45.9
10.6
2.1
30.6
4.9
6.6
1.3
0.1
Water Quality Parameters
2005
2010
2015
Temperature (°C)
15.0
13.9
21.3
Dissolved Oxygen (mg/L)
7.5
7.9
6.5
Specific Conductance (pS/cm)
27
88
96
pH (s.u.)
1 6.7
5.6
1 5.9
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
14
Bottom Substrate (15)
8
Pool Variety (10)
10
Riffle Habitat (16)
10
Bank Erosion (7)
5
Bank Vegetation (7)
6
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
78
Water Clarity Clear/Tannic
Site Photograph
,} f
Substrate: Cobble, gravel, sand
•zr
o'
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
05/27/15
2015-35
20
48
Good
04/29/10
2010-21
22
54
Excellent
05/02/05
2005-38
24
54
Excellent
06/22/00
2000-45
26
58
Excellent
10/31 /96
96-79
17
54
Excellent
07/21 /95
95-70
18
56
Excellent
06/06/91
91-11
21
54
Excellent
Data Analysis
Watershed -- drains northwest Johnston, eastern Wake (including the Town of Zebulon), southwest Nash, and southeast Franklin counties, including the US 64
and US 264 corridors; one NPDES permitted discharger in the watershed (NC0079316, located > 10 miles upstream); tributary to Buckhorn Reservoir
(Contentnea Creek). Habitats -- moderate to high quality habitats, has ranged from 78 to 88 since 1995; a few blow-out eroded banks; gravel and cobble riffles;
pools and side snags; wide and forested riparian zones. Water Quality -- except for 2005 (27 pS/cm), specific conductance has been relatively stable since
1991 ranging from 72 pS/cm to 96 pS/cm. 2015 -- fewest species collected since 1996; suckers absent again, only one species of sucker is known from this
site, V-lip Redhorse, and it is very rare, just 2 collected since 1991 (1 specimen each in 2000 and 2005); a 6-point decline in the NCIBI Score and one NCIBI
Rating class decrease were attributed to the absence of one species of darter (Chainback Darter or Glassy Darter), the absence of two or more species of
sunfish (except for Bluegill and Redbreast Sunfish all other species are rare at this site), and the absences of the intolerant Chainback Darter; none of these
metric declines are considered to be significant long-term declines in water quality. 1991-2015 -- community shares faunal similarities with Coastal Plain and
Piedmont fish communities; a very speciose community with 36 species known from the site (the most of any basinwide site in the Piedmont), including 11
species of sunfish, 7 species of cyprinids, 5 species of catfish, 4 species of darters, and 3 Intolerant Species (Pinewoods Shiner, Roanoke Darter, and
Chainback Darter); dominant species are White Shiner (1991, 1995, 2010, and 2015), Bluegill (1995 and 2005), and Roanoke Darter (1996 and 2000); several
metrics are very stable (Percentage of Tolerant Fish = 8%; Percentage of Omnivores+Herbivores = 10%, and Percentage of Insectivores = 86%); no substantial
change in the biological integrity or water quality of this stream. Recommendation -- continue basinwide monitoring of this site in 2020 to document any change
in its community in the face of the potential suburbanization of its watershed; with consistently high quality instream and riparian habitats, a fish community with 6
out of 7 Excellent ratings since 1991, and as a tributary to a water supply reservoir, this stream should be given priority for reclassification to High Quality
Waters.
Most Abundant o Non -indigenous Imperiled
Species White Shiner (63, 19 /o) Species Bluegill (20) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
NEWLIGHT CR
SR 1911
JF15
03/31/15
Good
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
36.027086
-78.601313
265
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
12.2
1 8
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
84.4
0.0
no data
13.6
no data
1.8
0.2
0.0
2001
69.7
4.7
0.4
17.5
4.8
1.4
0.2
0.1
2006
68.0
4.7
0.4
17.5
7.8
1.4
0.2
0.2
Water Quality Parameters
2005
2010
2015
Temperature (°C)
14.0
21.7
10.4
Dissolved Oxygen (mg/L)
10.4
10.2
13.2
Specific Conductance (pS/cm)
72
71
80
pH (s.u.)
1 6.8
1 6.6
1 6.5
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
9
Bottom Substrate (15)
3
Pool Variety (10)
4
Riffle Habitat (16)
4
Bank Erosion (7)
4
Bank Vegetation (7)
5
Light Penetration (10)
6
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
50
Water Clarity Clear
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
03/31 /15
2015-05
19
46
Good
04/06/10
2010-03
21
50
Good
04/04/05
2005-07
16
50
Good
05/16/95
95-43
16
42
Good -Fair
Data
Watershed -- drains south Granville and northern Wake counties; no NPDES permitted dischargers or municipalities in the watershed; tributary to Falls
Reservoir, site is -- 2 miles from the reservoir. Habitats -- lower quality instream habitats, has ranged from 47 to 61 since 2005 (no data were collected in
1995); very shallow with an abundance of periphyton; clay banks; sandy and gravelly runs; some side snags; no deep pools; beaver -impounded floodplains;
fairly open canopy, no instream coarse woody debris. Water Quality -- low flow; specific conductance during the last three cycles has ranged from 71 pS/cm to
80 pS/cm (data were not recorded in 1995). 2015 -- fewest fish ever collected at this site (n=209); only 1 species of darter and no Intolerant Species collected;
high percentage of diseased fish (2.9%, popeye disease in Bluegill caused by a nematode infection); a 4-point decline in the NCIBI Score, but not the NCIBI
Rating was due to declines in the number of fish, number of species of darters and Intolerant Species collected, and an elevated percentage of diseased fish
which were offset by a more balanced trophic structure and by a greater percentage of the species with multiple ages classes in 2015 than in 2010. 1995-2015
- a speciose community for the size of the stream, 28 species are known form the site, including 9 species of cyprinids, 6 species of sunfish, 3 species each of
suckers and darters, 2 Intolerant Species (Pinewoods Shiner and Roanoke Darter), and several migrants from the reservoir (e.g., Yellow Perch and White
Perch); dominant species are White Shiner (1995), Bluehead Chub (1995, 2005, 2015), Swallowtail Shiner (2005), and Spottail Shiner (2010); community has
rated Good since 2005; no substantial change in the biological integrity or water quality of this stream during the past three cycles. Recommendation --
continue basinwide monitoring of this site in 2020 to document potential suburbanization of its watershed.
Most Abundant Bluehead Chub (66, 32%) Non -indigenous Green Sunfish (6) and Imperiled None
Species Species Bluegill (16) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
N FLAT R
SR 1715
JF14
04/13/15
Good -Fair
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
PERSON
03020201
36.290000
-78.945278
495
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
L_ Carolina Slate Belt
33.0
10
0.4
Upstream NPDES Dischargers (? 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
60.2
7.2
no data
31.6
no data
0.3
0.5
0.1
2001
50.3
12.7
3.3
30.3
5.6
0.7
0.3
0.1
2006
50.1
12.9
3.5
30.2
5.7
0.8
0.3
0.1
2011
47.9
13.4
3.6
1 29.8
1 7.7
1 0.7
1 0.3
1 0.1
Water Quality Parameters
2005
2010
2015
Temperature (°C)
15.5
16.6
16.3
Dissolved Oxygen (mg/L)
9.8
10.2
10.5
Specific Conductance (pS/cm)
56
83
86
pH (s.u.)
---
6.4
6.7
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
18
Bottom Substrate (15)
10
Pool Variety (10)
8
Riffle Habitat (16)
14
Bank Erosion (7)
5
Bank Vegetation (7)
6
Light Penetration (10)
8
Left Riparian Score (5)
5
Right Riparian Score (5)
4
Total Habitat Score (100)
83
Water Clarity I Slightly turbid/tannic
Substrate: Carolina Slate Belt -type rocks, boulder, bedrock, cobble
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/13/15
2015-08
16
44
Good -Fair
04/27/10
2010-17
17
54
Excellent
04/06/05
2005-13
19
46
Good
04/06/00
2000-09
21
56
Excellent
06/10/99
99-45
18
50
Good
Data Analysis
Watershed -- drains south-central Person County, no NPDES permitted dischargers in the watershed, headwaters are in the southwest portion of the City of
Roxboro; tributary to the Flat River and Lake Michie. Habitat -- second highest quality habitats of any of the sites in the Neuse Piedmont in 2015; has ranged
from 83 to 92 since 1999; same as in previous cycles -- a typical high quality Carolina Slate Belt -type stream with angular rocks, slick with periphyton, riffles,
bedrock shelves, runs, pools, undercuts, and snags; Fissidens present; right riparian zone logged within the past few years; old mill site at the end of the reach.
Water Quality -- low flow; specific conductance within the norm for an eastern Neuse Piedmont stream, ranging from 72 pS/cm to 92 pS/cm, except during
2005 when it was measured at 56 pS/cm. 2015 -- most fish (n = 770) collected from any site in the Neuse Piedmont in 2015, four species of darters present,
low Percentage of Tolerant Fish (7%), but a high Percentage of Omnivores+Herbivores (65%, Bluehead Chub); the 10-point decline in the NCIBI Score and the
decline in two NCIBI Rating classes were due primarily to the very skewed and shift in trophic structure between 2010 and 2015 (from 32% to 65%
Omn ivores+ Herbivores and from 68% to 35% Insectivores); the changes in the latter two metrics, along with the increase in abundance of Bluehead Chub and
the periphyton-slick substrate, were indicative of some nutrient enrichment from nonpoint source runoff. 1999-2015 -- overall an abundant and diverse
community, but one whose fish abundance appears to gradually increasing since 2005 (from 485 to 675 to 770) while its species richness appears to be
gradually declining -- signs of nutrient enrichment and a change in water quality; 29 species are known from the site, including 7 species each of cyprinids and
sunfish, 5 species of suckers, 4 species of darters, and 3 Intolerant Species (Pinewoods Shiner, Roanoke Darter, a and Chainback Darter); dominant species
are the Bluehead Chub (1999-2015) and White Shiner (1999, 2000, 2010); there has been a 12-point decline in the NCIBI Score between 2000 and 2015.
Recommendation -- continue basinwide monitoring of this site in 2020 to document the increasing development of the watershed and to determine if the
decline in the NCIBI Rating is permanent or if it was an aberrant sample possibly due to the low flow conditions (confining the fish to a smaller area) because
prior to 2015, the ratings have varied between Good and Excellent.
Most Abundant Bluehead Chub (496, 64% Non -indigenous Green Sunfish (11) and Imperiled None
Species Species Bluegill (1) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
N FK LITTLE R
SR 1461
JF13
04/14/15
Good
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
DURHAM
03020201
36.163800
-78.948898
460
Yes
Level IV Ecoregion
Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Carolina Slate Belt
29.7
1 10
0.4
Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
72.8
0.1
no data
24.8
no data
1.1
0.4
0.9
2001
57.1
6.1
0.7
29.0
4.5
1.4
0.5
0.0
2006
57.0
6.1
0.7
28.9
6.1
1.4
0.5
0.0
Water Quality Parameters
2005
2010
2015
Temperature (°C)
17.8
16.7
18.3
Dissolved Oxygen (mg/L)
9.4
8.9
8.4
Specific Conductance (pS/cm)
77
90
96
pH (s.u.)
1 6.9
6.1
6.6
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
18
Bottom Substrate (15)
10
Pool Variety (10)
8
Riffle Habitat (16)
16
Bank Erosion (7)
6
Bank Vegetation (7)
6
Light Penetration (10)
9
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
88
Water Clarity I Slightly turbid, very easily silted
Substrate: Carolina Slate Belt type rocks, cobble, boulder, bedrock, gravel
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/14/15
2015-11
16
50
Good
04/27/10
2010-16
17
50
Good
04/07/05
2005-17
16
52
Good
04/07/00
2000-13
14
48
Good
Data
Watershed -- drains north -central Orange and northwest Durham counties; no NPDES permitted dischargers or municipalities in the watershed; tributary to
Little River Reservoir, site is - 4.8 miles upstream from the backwaters of the reservoir. Habitat -- highest quality habitats of any of the sites in the Neuse
Piedmont in 2015; has ranged from 87-93 since 2000; no change since 2000 reflecting stable landuse in the watershed; a typical high quality Carolina Slate
Belt -type stream with riffles, runs, pools, Fissidens, rocks slick with periphyton making sampling difficult. Water Quality -- low flow; specific conductance has
ranged from 72 pS/cm to 96 pS/cm and continued to be within the norm for an eastern Piedmont stream. 2015 -- a slight decline in the number of species of
darters, suckers, and Intolerant Species (one less species of each) were offset by a slightly more balanced trophic structure in 2015 than in 2010; only site in
the Neuse Piedmont from which Swallowtail Shiner (a wide -spread species) was not collected; no changes in the NCIBI Score or NCIBI Rating. 2000-2015 --
25 species known from the site, including 6 species of cyprinids, 5 species of sunfish, 4 species of suckers, 3 species of darters (although the Fantail Darter
was absent), the endemic Roanoke Bass, and 3 Intolerant Species (Pinewoods Shiner, Roanoke Darter, and Chainback Darter); dominant species has
consistently been the Bluehead Chub; no substantial change in the biological integrity or water quality of this stream which has consistently rated Good since
2000; metrics are stable with every sampling effort represented by 2 or 3 species of darters, 4 species of sunfish, 44% Omnivores+Herbivores, and 54%
Insectivores, the latter two metrics, along with the periphyton-slick substrate, consistently showing some nutrient enrichment from nonpoint source runoff.
Recommendation -- continue basinwide monitoring of this regional reference site in 2020 to document its support of its supplemental classification as High
Quality Waters in the face of the potential suburbanization of the watershed.
Most Abundant Bluehead Chub (148, 35% Non -indigenous Green Sunfish (15) and I Imperiled Significantly Rare - Roanoke Bass (5)
Species Species Bluegill (1) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclass ification
RICHLAND CR
US 1
JF29
03/31/15
Good -Fair
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
35.961667
-78.542500
220
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
10.5
1 7
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
70.7
13.7
no data
14.8
no data
0.2
0.6
0.0
2001
48.5
28.5
6.0
12.6
8.7
0.4
0.6
0.8
2006
44.9
34.8
8.0
11.6
6.9
0.3
0.5
1.0
2011
42.1
39.1
9.9
9.6
7.8
0.3
0.5
0.6
Water Quality Parameters
2010
2011
2015
Temperature (°C)
16.9
24.0
7.8
Dissolved Oxygen (mg/L)
8.3
5.6
12.1
Specific Conductance (pS/cm)
104
116
102
pH (s.u.)
6.1
6.4
6.5
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
9
Bottom Substrate (15)
3
Pool Variety (10)
8
Riffle Habitat (16)
2
Bank Erosion (7)
1
Bank Vegetation (7)
4
Light Penetration (10)
4
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
46
Water Clarity I Clear, but easily silted
Substrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
03/31 /15
2015-03
13
44
Good -Fair
06/02/11
2011-26
16
48
Good
04/07/10
2010-04
14
42
Good -Fair
04/04/05
2005-08
18
54
Excellent
04/12/95
95-20
18
52
Good
Data
Watershed -- drains an area west of the cities of Wake Forest and Youngsville in northeast Wake and southwest Franklin counties, including the US 1 (Capital
Blvd.) and NC 98 corridors; no NPDES permitted dischargers in the watershed; an extremely hydrologically flashy creek that is a tributary to the Neuse River,
site is - 2.7 miles upstream of the creek's confluence with the river. Habitats -- second lowest quality habitats of any of the sites in the Neuse Piedmont in
2015, range 38-53 since 2005; deeply entrenched with massive erosion and sloughing banks; a shifting and sinuous channel with an abundance of large,
coarse woody debris across the channel; good snags and bank undercuts; stick riffles. Water Quality-- specific conductance has been > 100 pS/cm during the
past three monitoring periods, indicative of an increasingly urbanized watershed; has ranged from 74 pS/cm to 116 pS/cm since 1995. 2015 -- a 4-point decline
in the NCIBI Score and one NCIBI Rating class decrease were attributed to the lower than expected total species richness and the absence of darters and
suckers. 1995-2015 -- 25 species are known from the site, including 7 species of cyprinids, 5 species of sunfish, 4 species of suckers, 2 species of darters, but
no Intolerant Species; dominant species are Bluegill and Bluehead Chub (1995) and White Shiner (2005-2011), and Speckled Killifish (2015); three species of
suckers have not been collected since 2005 (Creek Chubsucker) or 2010 (Notchlip Redhorse and V-Lip Redhorse); the occurrence and abundance of the
piscivorous, anadromous American Eel which, in part, affects the NCIBI Scores and NCIBI Ratings, along with the absence of other species (Pinewoods Shiner
and Roanoke Darter), is negatively influenced by the continued existence of the Milburnie Dam downstream on the Neuse River (slated for possible removal in
the near future); the Percentage of Tolerant Fish seems to be gradually increasing from 14% in 1995 to 30% in 2015, but the trophic metrics (Percentage of
Omnivores+Herbivores and Percentage of Insectivores) have been relatively stable; NCIBI Scores and Ratings have ranged from Good -Fair to Excellent since
1995. Recommendation -- continue basinwide monitoring of this site in 2020 to document continuing urbanization of the watershed.
Most Abundant Speckled Killifish (142, Non -indigenous Green Sunfish (9) and Imperiled
Species 42%) Species Bluegill (4) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
SMITH CR
SR 1710
JF19
03/30/15
Good -Fair
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
GRANVILLE
03020201
36.088333
-78.602222
310
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Triassic Basins
6.2
6
1 0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
88.2
0.1
no data
8.6
no data
2.8
0.2
0.1
2001
74.6
4.1
0.4
10.3
7.2
2.3
0.2
0.0
2006
74.0
4.1
0.4
10.0
9.2
2.4
0.2
0.0
Water Quality Parameters
2005
2010
2015
Temperature (°C)
11.7
18.8
7.4
Dissolved Oxygen (mg/L)
10.6
8.1
12.8
Specific Conductance (pS/cm)
67
75
77
pH (s.u.)
6.8
6.1
5.9
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
12
Bottom Substrate (15)
4
Pool Variety (10)
7
Riffle Habitat (16)
7
Bank Erosion (7)
2
Bank Vegetation (7)
6
Light Penetration (10)
8
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
61
Site Photograph
r
,
All
.. .^ 7
Water Clarity I Slightly turbid Substrate: Gravel, sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
03/30/15
2015-01
16
42
Good -Fair
04/06/10
2010-02
14
44
Good -Fair
04/04/05
2005-06
13
42
Good -Fair
04/04/00
2000-03
15
44
Good -Fair
04/11 /95
95-18_1
20
48
Good
Data
Watershed -- a small watershed draining southeastern Granville County; no NPDES dischargers or municipalities in the watershed; greatest percentage of its
watershed in forest and least amount developed and impervious cover than any other Neuse Piedmont watershed in 2015; tributary to Falls Reservoir; as a
Triassic Basins stream it may go intermittent or dry up during prolonged droughts. Habitats -- moderate quality habitats, same as in 2005 and 2010 (range 56-
72 since 2000) with coarse woody debris deadfalls and side snags; riffles, runs, and snag and undercut pools; short, gravel riffles; eroded and entrenched right
bank. Water Quality -- specific conductance stable, has ranged from 67 pS/cm to 77 pS/cm during the past four cycles (a lower value - 52 pS/cm) was
recorded in 1995) and has been within the norm for an eastern Piedmont stream. 2015 -- as in years past an abundant community (n=354), but with a very low
percentage of species with multiple age classes (only 31 %; 8 of the 16 species were represented by only 1 or 2 fish, indicative of a loss of age classes either
due to drought or high flows); many Age 1 Bluehead Chub, White Shiner, and Swallowtail Shiner which also indicated a re -populating of the stream, these three
species represented 86% of all the fish collected. 1995-2015 -- for its size stream, a very speciose fish community with 25 species known from this site,
including 8 species of sunfish, 6 species of cyprinids, 2 species each of suckers and darters, but no Intolerant Species; Redfin Pickerel, Northern Hog Sucker,
Eastern Mosquitofish, and Black Crappie have not been collected since 1995; dominant species are the White Shiner (2000, 2010, and 2015) and Bluehead
Chub (1995 and 2005); no substantial changes in the biological integrity or water quality of this stream since 2000. Recommendation -- continue basinwide
monitoring of this site to document the potential urbanization of the watershed.
Most Abundant White Shiner (169, 48%) Non -indigenous Green Sunfish (1) and Imperiled None
Species Species Bluegill (4) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
SMITH CR
SR 2045
JF31
03/31/15
Good -Fair
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
35.918360
-78.534543
190
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
22.6
10
1 0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile)
NPDES Number
Volume (MGD)
Whippoorwill Valley WWTP
NCO073318
0.2
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
66.8
5.9
no data
24.5
no data
1.8
0.9
0.0
2001
51.6
22.5
4.2
15.9
no data
1.6
0.9
0.3
2006
44.2
31.2
6.5
14.4
no data
1.4
0.8
no data
2011
40.9
36.1
8.6
13.1
2.1
1.4
0.8
no data
Water Quality Parameters
2005
2010
2015
Temperature (°C)
11.8
17.9
8.3
Dissolved Oxygen (mg/L)
9.4
8.5
12.2
Specific Conductance (pS/cm)
92
104
104
pH (s.u.)
6.4
6.3
6.3
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
11
Bottom Substrate (15)
3
Pool Variety (10)
4
Riffle Habitat (16)
2
Bank Erosion (7)
5
Bank Vegetation (7)
6
Light Penetration (10)
8
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
54
Water Clarity IClear
Substrate: Sand, gravel
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
03/31 /15
2015-04
24
44
Good -Fair
04/07/10
2010-05
21
50
Good
04/05/05
2005-09
23
36
Fair
04/03/00
2000-02
17
56
Excellent
05/18/95
95-45
14
42
Good -Fair
Data
Watershed -- drains the City of Wake Forest and surrounding suburbs in northeast Wake and southwest Franklin counties; one NPDES permitted discharger in
the watershed (NC0073318, QW = 0.2 MGD) located - 1.1 miles upstream; tributary to the Neuse River, site is - 0.8 miles above the creek's confluence with the
river. Habitats -- no change in the low quality riparian or instream habitats since the last cycle, has ranged from 46 to 66 since 1995; shallow, soft sandy runs
of uniforms depth; stick riffles; side snag pools. Water Quality -- despite the increasingly developed watershed and the upstream discharger, the specific
conductance has been very stable, ranging from 90 pS/cm to 104 pS/cm. 2015 -- second most species (n = 24) collected at any site in the Neuse Piedmont in
2015; a 6-point decline in the NCIBI Score and one NCIBI Rating class decrease were attributed to the loss of two species of suckers (Northern Hog Sucker
and Notchlip Redhorse) and the very high Percentage of Tolerant Fish (60%, primarily Satinfin Shiner and Green Sunfish). 1995-2015 -- community shares
some faunal similarities with Coastal Plain fish communities (e.g. Flier, Eastern Mudminnow, Tadpole Madtom, Yellow Perch, and Bluespotted Sunfish); a very
speciose community with 35 species known from the site (the second most of any basinwide site in the Neuse Piedmont), including 10 species of sunfish, 7
species of catfish, 6 species of cyprinids, 3 species each of darters and suckers, but just 1 Intolerant Species (Chainback Darter; Pinewood Shiner and and
Roanoke Darter are absent); the absence of some species (e.g., Pinewoods Shiner and Roanoke Darter) are due in part to the continued existence of the
Milburnie Dam (slated for possible removal in the near future) downstream on the Neuse River; the site's proximity to the river may also influence the
community's overall diversity and abundance (i.e. readily available avenues for recolonization following droughts and other perturbations and seasonal
migrations to and from the river); dominant species are Bluehead Chub (1995), Eastern Mosquitofish (2005), Swallowtail Shiner (2000 and 2010), and Satinfin
Shiner (2015); since 1995, the dominance of Bluehead Chub has been steadily decreasing (from 45% in 1995 to 2% in 2015), while the dominance of the
tolerant Satinfin Shiner has been steadily increasing (from 5% to 50%), perhaps indicating a shift from coarse gravel to a finer sand substrate; since 2005 the
trophic structure has been skewed, being dominated more by Insectivores (92%) and less by Omnivores+Herbivores (- 7%); NCIBI Scores and NCIBI Rating
have ranged from 36 (Fair) to 56 (Excellent). Recommendation -- continue basinwide monitoring of this site in 2020 to document the watershed's increasing
urbanization.
Most Abundant Satinfin Shiner (241, 50%) Non -indigenous Green Sunfish (17) and Imperiled None
Species ISpecies Bluegill (17) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
S FLAT R
NC 157
JF18
04/13/15
Good
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
PERSON
03020201
36.277500
-79.045000
550
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Southern Outer Piedmont
17.3
1 9
0.4
Upstream NPDES Dischargers (Z 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I ---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
61.6
2.0
no data
35.6
no data
0.2
0.5
0.0
2001
50.0
4.6
0.7
38.2
5.0
0.4
0.4
0.5
2006
50.3
4.6
0.7
38.2
5.7
0.4
0.4
0.5
Water Quality Parameters
2005
2010
2015
Temperature (°C)
18.0
13.9
17.8
Dissolved Oxygen (mg/L)
9.0
8.2
10.8
Specific Conductance (pS/cm)
83
91
104
pH (s.u.)
---
5.9
6.6
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
12
Bottom Substrate (15)
5
Pool Variety (10)
8
Riffle Habitat (16)
11
Bank Erosion (7)
3
Bank Vegetation (7)
6
Light Penetration (10)
8
Left Riparian Score (5)
3
Right Riparian Score (5)
5
Total Habitat Score (100)
66
Water Clarity I Slightly turbid Substrate: Carolina Slate Belt -type rocks above the bridge; sand and gravel below the bridg
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/13/15
2015-09
22
46
Good
04/23/10
2010-14
24
48
Good
04/06/05
2005-14
18
48
Good
04/06/00
2000-10
17
48
Good
Data
Watershed -- drains south-central and southwest Person County; no NPDES permitted dischargers or municipalities in the watershed; greatest percentage of
its watershed under cultivation than any other Neuse Piedmont watershed (along with Little River) in 2015; site is below historic Hurdle Mills; tributary to Flat
River and Lake Michie. Habitats -- moderate quality habitats, range 59 to 66 since 2000; same as in 2005 and 2010 -- two different habitat zones: above the
bridge - high quality Carolina Slate Belt -type stream with angular rocks, riffles, Podostemum; thick and nuisance levels of periphyton abundant; below the
bridge - sandy runs and pools with some severely entrenched banks below the bridge. Water Quality -- low flow; specific conductance has ranged from 69
pS/cm to 104 pS/cm during the last four cycles and has been within the norm for an eastern Piedmont stream. 2015 -- a 2-point difference in the NCIBI Score
was due to the absence of the intolerant Chainback Darter which is very rare at this site, only 1 specimen has been collected since 2000; skewed trophic
metrics (39% Omnivores+Herbivores and 60% Insectivores) again indicated an excess of nutrients, and filamentous algae and periphyton are plentiful in the
stream. 2000-2015 -- a very speciose fish community given the size of the stream, 29 species are known from the site including 9 species of cyprinids, 8
species of sunfish, 3 species each of suckers and darters, and 3 Intolerant Species (Pinewoods Shiner, Roanoke Darter, and Chainback Darter); several
metrics are stable with every sampling effort represented by 2 or 3 species of darters, 4-6 species of sunfish, 1 or 2 species of suckers, 42%
Omnivores+Herbivores, and 58% Insectivores, the latter two metrics, along with the dominance by Bluehead Chub and the periphyton-slick substrate, are
indicative of nutrient enrichment from nonpoint source runoff; consistently dominant species is the Bluehead Chub; site has rated Good since 2000; no
substantial change in the biological integrity or water quality of this stream. Recommendation -- continue basinwide monitoring of this site in 2020 to docume
the potential suburbanization of the watershed.
Most Abundant Bluehead Chub (121, 38% Non -indigenous Green Sunfish (15) and Imperiled None
Species ISpecies Bluegill (12) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
S FK LITTLE R
SR 1461
JF17
04/14/15
Excellent
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
DURHAM
03020201
36.149722
-78.946389
425
Yes
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Carolina Slate Belt
39.0
9
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
71.3
0.7
no data
27.2
no data
0.5
0.3
0.0
2001
58.7
5.4
0.5
29.7
3.7
0.8
0.4
0.1
2006
59.1
5.4
0.5
29.8
4.5
0.8
0.4
0.1
Water Quality Parameters
2005
2010
2015
Temperature (°C)
17.1
16.5
18.7
Dissolved Oxygen (mg/L)
9.4
9.0
9.8
Specific Conductance (pS/cm)
83
97
102
pH (s.u.)
1 6.8
6.6
1 7.0
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
16
Bottom Substrate (15)
11
Pool Variety (10)
10
Riffle Habitat (16)
10
Bank Erosion (7)
6
Bank Vegetation (7)
5
Light Penetration (10)
7
Left Riparian Score (5)
2
Right Riparian Score (5)
2
Total Habitat Score (100)
74
Musa
Water Clarity IClear Substrate: Carolina Slate Belt type rocks, cobble, boulder, gravel
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/14/15
2015-12
21
58
Excellent
04/27/10
2010-15
19
58
Excellent
04/07/05
2005-16
20
60
Excellent
04/07/00
2000-12
24
60
Excellent
Data
Watershed -- drains north -central Orange and northwest Durham counties; no NPDES permitted dischargers or municipalities in the watershed; tributaryto
Little River Reservoir, site is - 3.2 miles upstream from the reservoir's backwaters. Habitats -- moderate -high quality instream habitats, has ranged from 74 to
87 since 2000; no change since last cycle; right bank eroding; a typical Carolina Slate Belt -type stream with periphyton covered -slick rocks, shallow riffles, a
very deep pool at end of reach, and side undercuts and snags; heavy red algal growths; narrow riparian zones. Water Quality -- low flow; specific conductance
during the last four cycles has ranged from 79 pS/cm to 102 pS/cm and within the norm for an eastern Piedmont stream. 2015 -- second most fish (n=654)
collected from any site in the Neuse Piedmont in 2015; number of fish collected has been increasing in every cycle (n = 361, 485, 566, and 654, respectively)
since 2000; highest NCIBI Score and NCIBI Rating (along with Horse Creek) of any site in the Neuse Piedmont in 2015; only metric not scoring a maximum of
"5' was the Percentage of Piscivores (0.76% = "3" ); most species collected since 2000; collected 16 large specimens (- 450-550 mm TL) of ready -to -spawn
male and female Notchlip Redhorse. 2000-2015 -- 26 species known from the site, including 7 species of sunfish, 6 species of cyprinids, 4 species of suckers,
3 species of darters (but not the Fantail Darter), the endemic Roanoke Bass, and 3 Intolerant Species (Pinewoods Shiner, Roanoke Darter, and Chainback
Darter); dominant species have been Redbreast Sunfish and Bluehead Chub; no substantial change in the biological integrity or water quality of this stream
which has consistently rated Excellent since 2000; metrics are extremely stable with every sampling effort represented by 3 species of darters, 4-6 species of
sunfish, 3 or 4 species of suckers, 3 Intolerant Species, 27% Tolerant Fish, 20% Omnivores+Herbivores, and 77% Insectivores. Recommendation -- continue
basinwide monitoring of this regional reference site in 2020 to document its support of its supplemental classification of High Quality Waters in the face of the
potential suburbanization of the watershed.
Most Abundant Redbreast Sunfish (128, Non -indigenous Green Sunfish (33) and Imperiled SignificantlyRare - Roanoke Bass 3
Species 20%) Species Bluegill (1) Species ( )

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassi ication
SWIFT CR
SR 1152
JF91
04/28/15
Fair
County 8 digit HUC Latitude Longitude Elevation t Re erence ite
WAKE 03020201 35.718950-78.752500 300 No
Level IV Ecoregion Drainage Area miStream Width m Stream Depth m
Northern Outer Piedmont 21.0 10 0.4
Upstream NPDES Dischargers >_1 M D or < 1 M D and within I mile NPDES Number Volume M D
None
Landuse %
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
54.9
33.2
no data
7.9
no data
1.3
2.2
0.6
200
20.3
74.0
14.6
2.1
0.7
0.7
1.8
0.1
2006
18.4
75.2
15.5
2.3
1.8
0.7
.8
0.1
201
6.6
78.3
17.2
1.2
1.5
0.7
.7
0.0
Water Quality Parameters
2000
2010
2015
Temperature (°C)
15.0
19.7
13.2
Dissolved Oxygen (mg/L)
7.3
5.2
8.7
Specific Conductance (pS/cm)
135
140
130
pH (s.u.)
6.7
6.0
1 6.3
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
12
Bottom Substrate (15)
4
Pool Variety (10)
8
Riffle Habitat (16)
3
Bank Erosion (7)
1
Bank Vegetation (7)
6
Light Penetration (10)
9
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
58
Water Clarity I Slightly turbid-turbid,darkwater
ubstrate: Sand, gravel
Sample Date
Sample ID
Species Total
N IBI Score
N IBI Rating
04/28/15
2015-15
15
34
Fair
04/08/10
2010-07
15
40
Good -Fair
04/24/00
2000-16
13
34
Fair
04/24/00
2000-17
18
40
Good -Fair
10/15/99
99-66
10
34
Fair
10/15/99
99-67
12
40
Good -Fair
08/20/99
99-58, 99-59
15,15
38,38
Fair, Fair
06/25/99
99-56
14
38
Fair
06/25/99
99-57
16
40
Good -Fair
04/28/99
99-26
16
42
Good -Fair
04/28/99
99-27
13
38
Fair
04/27/95
95-29
9
28
Poor
Data
Watershed -- drains central Wake County, including areas of the cities of Apex and Cary; golf course immediately upstream; no NPDES permitted dischargers
in the watershed; site is -1.5 miles upstream from the backwaters of Lake Wheeler; tributary to Neuse River. Habitats -- low to moderate quality habitats; has
ranged from 58 to 78 since 1995; large, coarse woody debris snags, deadfalls, and dams; gravel riffles; severe bank erosion; deeply entrenched; hydrologically
flashy due to the high percentage of development in its watershed. Water Quality -- specific conductance has ranged from 78 pS/cm in 1999 to 140 pS/cm in
2010; since 2000 measurements have been >_ 130 pS/cm and are indicative of stormwater runoff from an increasingly urbanized and developed watershed.
2015 -- lower than expected total abundance and species richness including suckers and darters; high percentage of diseased fish (4.2%, popeye disease in
Bluegill caused by a nematode infection); as in all previous cycles, there was a very skewed trophic structure with an almost complete absence of
Omnivores+Herbivores and an abundance of Insectivores (primarily sunfish, Eastern Mosquitofish, and Swallowtail Shiner). 1995-2015 -- 25 species are
known from the site, including 8 species of sunfish, 2 species of suckers, but only 1 species of darter; the wide -spread Bluehead Chub, Satinfin Shiner, and
Intolerant Species are absent; two-thirds of all fish collected since 1995 have been sunfish, typical of a stressed fish community in an urban stream and one that
is in close proximity to a reservoir; the dominant species from 1995-2000 and 2015 was Bluegill and in 2010 it was the Swallowtail Shiner; since 1995, 8 out of
the 13 samples have rated Fair or Poor. Frequently observed in urban streams with degraded water quality, there is am absence or a decrease in the
abundance of the omnivorous Bluehead Chub, there is an absence of Intolerant Species, low diversities of darters and suckers, and the community becomes
dominated by insectivores and by tolerant species such as the Redbreast Sunfish, ultimately leading to depressed NCIBI metric scores and ratings compared to
regional reference sites. Recommendation -- until improved stormwater controls are implemented throughout the watershed, continued fish community
monitoring is unnecessary, except for once every 10 years as no significant improvements are to be expected.
-1 i
Most Abundant Bluegill (97, 45%) Non -indigenous Green Sunfish (11), Bluegill Imperiled None
Species Species (97), and Redear Sunfish (3) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
TERRIBLE CR
SR 2751
JF35
03/30/15
Good -Fair
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
35.614444
-78.725000
260
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
11.0
6
1 0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile)
NPDES Number
Volume (MGD)
Town of Fuquay-Varina's Terrible Creek WWTP
NCO066516
6
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
43.8
7.0
no data
44.7
no data
2.5
1.9
0.0
2001
29.8
23.4
5.2
25.9
14.0
4.3
1.2
0.0
2006
26.9
31.2
7.6
22.7
13.3
4.3
1.2
0.1
2011
1 26.1
34.6
8.5
1 21.3
12.6
1 4.2
1 1.1
L0.4
Water Quality Parameters
2005
2010
2015
Temperature (°C)
17.7
16.0
10.3
Dissolved Oxygen (mg/L)
8.0
7.6
12.2
Specific Conductance (pS/cm)
97
138
139
pH (s.u.)
6.2
6.5
6.3
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
14
Bottom Substrate (15)
4
Pool Variety (10)
9
Riffle Habitat (16)
10
Bank Erosion (7)
4
Bank Vegetation (7)
6
Light Penetration (10)
8
Left Riparian Score (5)
3
Right Riparian Score (5)
5
Total Habitat Score (100)
68
Site Phot
Water Clarity I Slightly turbid/tannic I ISubstrate: Gravel, sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
03/30/15
2015-02
14
44
Good -Fair
04/21 /10
2010-10
17
52
Good
04/08/05
2005-18
14
50
Good
Data
Watershed -- drains southeast Wake County northeast of the city of Fuquay-Varina, including the US 401 corridor; greatest percentage of its watershed in
grassland/herbaceous/shrub than any other watershed in the Neuse Piedmont in 2015; one NPDES permitted discharger in the watershed (NC0066516,
located - 0.3 miles upstream); tributary to Middle Creek. Habitats --moderate quality habitats, same as in 2005 and 2010 (range 61-69 since 2005); strong,
swift flow; gravel riffles; eroding banks on the left with blowouts; coarse woody debris and deadfalls creating pools; wide bottomland riparian zones in much of
the reach; evidence of past very high water; becoming a more hydrologically flashy stream as its watershed is being increasingly developed. Water Quality -- a
detectable odor of chlorine from the WWTP effluent; specific conductance elevated from the effluent and has ranged from 97 pS/cm in 2005 to 139 pS/cm in
2015. 2015 -- second fewest fish (n = 111) collected from any site in the Neuse Piedmont in 2015, 628 fish were collected in 2010; a 6-point decline in the
NCIBI Score and one NCIBI Rating class decrease were attributed to the lower than expected total species richness, fewer fish, the loss of two species of
intolerant darters (Roanoke Darter and Chainback Darter), and the loss of three species of sunfish (Warmouth, Redear Sunfish, and Largemouth Bass);
collectively these metric decreases and the visible appearance of the site indicated that very high water might have scoured out the stream and re -structured
the fish community, in fact between late December 2014 and mid -January 2015 there were two very high flow events (e.g., Middle Creek near Clayton was >
2,500 cfs in one event and - 2,000 cfs in the second event while the median flow was - 75 cfs). 2005-2015 -- 23 species are known from the site, including 7
species of sunfish, 6 species of cyprinids, 3 species of darters, 3 Intolerant Species (Pinewoods Shiner, Roanoke Darter, and Chainback Darter), and 2 species
of sucker; dominant species are Bluehead Chub (2005) and Redbreast Sunfish (2010 and 2015); community appears to have shifted since 2005 from Bluehead
Chub, White Shiner, and Swallowtail Shiner (66% to 42% to 16%) to Redbreast Sunfish (11 % to 38% to 35%) with the the Percentage of Tolerant Fish
increasing from 12% to 38% to 43%. Recommendation -- continue basinwide monitoring of this site in 2020 to continue document impacts from the expansion
of the WWTP discharge from 1 MGD to 6 MGD and the increasing suburbanization of the watershed.
Most Abundant Redbreast Sunfish (39, Non -indigenous Imperiled
SSpecies 35% Bluegill (21) None
p ) Species Species S

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
UPPER BARTON CR
NC 50
JF21
04/15/15
Good -Fair
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
35.951910
-78.687482
295
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth
Northern Outer Piedmont
5.8
5
0.3 !��d
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
87.1
10.1
no data
0.8
no data
1.8
0.2
0.0
2001
70.1
22.1
1.9
2.6
2.1
1.8
0.1
0.0
2006
69.5
22.2
1.9
2.6
3.8
1.8
0.1
0.0
Water Quality Parameters
2005
2010
2015
Temperature (°C)
10.8
18.1
16.4
Dissolved Oxygen (mg/L)
9.8
7.6
8.5
Specific Conductance (pS/cm)
95
169
207
pH (s.u.)
1 6.7
1 6.2
1 6.4
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
12
Bottom Substrate (15)
4
Pool Variety (10)
8
Riffle Habitat (16)
5
Bank Erosion (7)
2
Bank Vegetation (7)
6
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
62
Site Photograph
r -
Water Clarity I Slightly turbid, easily silted I ISubstrate: Cobble, gravel, bedrock shelves, sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/15/15
2015-13
17
42
Good -Fair
04/06/10
2010-01
16
44
Good -Fair
04/04/05
2005-05
14
46
Good
04/03/00
2000-01
21
52
Good
05/18/95
95-44
17
48
Good
Data
Watershed -- drains the increasingly urbanized portion of northwest Wake County, west of NC 50 (Creedmoor Road); smallest watershed that was assessed in
the Piedmont in 2015; one NPDES permitted discharger in the watershed (NC0049662, Hawthorn Subdivision WWTP, QW = 0.25 MGD, located -3.8 miles
upstream); tributary to Falls Reservoir. Habitats -- low to moderate quality habitats, has ranged from 57 to 87 since 1995; gravel riffles; side snags and side
pools; more sediment and shifting gravel bars in the channel than during the last cycle; eroding banks; bedrock ledges. Water Quality -- elevated specific
conductance due to upstream discharger, nonpoint runoff, and stormwater runoff; has increased dramatically during the past three cycles from 95 pS/cm to 207
pS/cm, was 120 pS/cm and 144 pS/cm in 1995 and 2000, respectively. 2015 -- although only 422 fish were collected in 2015 in contrast to 1,284 collected in
2010, the community was still an extremely abundant fish community for such a small stream; the abundance of Bluehead Chub and periphyton and the altered
trophic structure (62% Omnivores+Herbivores and 38% Insectivores) indicated point and nonpoint sources nutrient enrichment; 12 large male and female
Notchlip Redhorse (420-560 mm TL) were collected. 1995-2015 -- very speciose community for the small stream size, 28 species are known from the site,
including 7 species each of cyprinids and sunfish and 3 species each of suckers and darters, but just 1 Intolerant Species (Pinewoods Shiner and Chainback
Darter have not been collected in this stream); the intolerant Roanoke Darter has not been collected since 1995 and other widespread species such as
Margined Madtom and Satinfin Shiner have not been collected since 2000; the high taxa richness may be due in part to the site's proximity to the reservoir with
several species migrating to and from the reservoir (e.g., Yellow Perch, Spottail Shiner, and Notchlip Redhorse); dominant species are the Bluehead Chub
(1995-2015) and White Shiner (1995-2005); a very productive stream with the number of fish being collected ranging from 422 to 1,284; NCIBI Score has
declined by 10-points since 2000 and the community has rated Good -Fair since 2010. Recommendation -- continue basinwide monitoring of this site in 2020
to document the watershed's increasing urbanization.
Most Abundant Bluehead Chub (208, 49% Non -indigenous Green Sunfish (10) and Imperiled None
Species Species Bluegill (9) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
WALNUT CR
South State Street
JF104
04/28/15
Fair
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
35.757937
-78.623773
215
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
23.4
10
0.4
Upstream NPDES Dischargers (? 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
27.9
59.1
no data
2.7
no data
1.3
1.8
7.1
2001
14.9
80.1
21.9
1.8
0.7
0.8
1.6
0.1
2006
13.8
81.1
23.3
1.6
1.1
0.8
1.6
0.2
2011
1 12.6
82.6
24.6
1 1.5
0.8
1 0.7
1 1.5
1 0.1
Water Quality Parameters
2010
2015
Temperature (°C)
---
15.8
14.3
Dissolved Oxygen (mg/L)
---
7.1
9.0
Specific Conductance (pS/cm)
---
187
169
pH (s.u.)
---
5.5
6.2
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
4
Instream Habitat (20)
9
Bottom Substrate (15)
3
Pool Variety (10)
10
Riffle Habitat (16)
0
Bank Erosion (7)
3
Bank Vegetation (7)
5
Light Penetration (10)
7
Left Riparian Score (5)
5
Right Riparian Score (5)
5::::]
Total Habitat Score (100)
51
Site Photograph
Water Clarity IClear Substrate: Sand, urban debris
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/28/15
2015-16
17
36
Fair
04/21 /10
2010-12
16
34
Fair
Data
Watershed -- drains central Wake County and the southern area of the City of Raleigh and the eastern area of the City of Cary; second greatest percentage of
its watershed developed than any other Neuse Piedmont watershed in 2015; no NPDES permitted dischargers in the watershed; site is - 4 miles below Lake
Raleigh which has no minimum flow requirement; tributary to the Neuse River. Habitats -- low quality habitats, was scored 51 in 2010 and 2015; a flashy and
entrenched urban stream with the channel, banks, and riparian zones littered with trash and illegally dumped tires; eroded banks; large woody debris, side
snags, and deadfalls; deep pools. Water Quality -- specific conductance elevated due to nonpoint and stormwater runoff from a highly urbanized and
impervious watershed. 2015 -- suckers and Intolerant Species absent; very skewed trophic structure (96% Insectivores and 2% Omnivores+Herbivores); high
percentage of diseased fish (3.1 %, popeye disease in Bluegill caused by a nematode infection). 2010 & 2015 -- 23 species known from the site, including 6
species of sunfish, 7 species of cyprinids, but just 1 species each of suckers and darters, and no Intolerant Species; skewed trophic structure, and high
incidence of disease in Bluegill. Frequently observed in urban streams with degraded water quality, there is am absence or a decrease in the abundance of the
omnivorous Bluehead Chub, there is an absence of Intolerant Species, low diversities of darters and suckers, and the community becomes dominated by
insectivores and by tolerant species such as the Redbreast Sunfish, ultimately leading to depressed NCIBI metric scores and ratings compared to regional
reference sites. Recommendation -- the cites of Cary and Raleigh, along with Wake County, should initiate a stream cleanup to remove urban debris illegally
dumped into the stream and carried into the stream by stormwater runoff; until watershed stormwater controls are improved or expanded, continued monitoring
of the impaired fish community is unnecessary, except for once every 10 years.
Most Abundant Swallowtail Shiner (139, Non -indigenous Bluegill (9) and Fathead Imperiled
Species 44%) Species Minnow (1) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
WALNUT CR
SR 2544
JF32
05/21/15
Poor
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAKE
03020201
35.758315
-78.582892
190
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
29.4
8
0.5
Upstream NPDES Dischargers (? 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
27.7
59.7
no data
3.0
no data
2.5
1.4
5.6
2001
14.5
80.0
20.7
1.7
0.5
1.7
1.3
0.1
2006
13.2
81.1
21.3
1.5
1.0
1.6
1.3
0.2
Water Quality Parameters
2005
2010
2015
Temperature (°C)
15.3
20.1
21.1
Dissolved Oxygen (mg/L)
8.5
7.6
6.7
Specific Conductance (pS/cm)
144
206
208
pH (s.u.)
6.8
6.3
6.5
Habitat Assessment Scores (max score)
2014
Channel Modification (5)
5
Instream Habitat (20)
10
Bottom Substrate (15)
3
Pool Variety (10)
10
Riffle Habitat (16)
1
Bank Erosion (7)
3
Bank Vegetation (7)
6
Light Penetration (10)
8
Left Riparian Score (5)
4
Right Riparian Score (5)
4
Total Habitat Score (100)
54
Water Clarity I Slightly turbid
Substrate: Sand, bedrock, boulder
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
05/21 /15
2015-32
12
32
Poor
04/08/10
2010-09
18
40
Good -Fair
04/05/05
2005-11
17
44
Good -Fair
04/11 /00
2000-14
18
44
Good -Fair
04/04/95
95-13
12
34
Fair
06/25/91
91-15
20
48
Good
Data Analysis
Watershed -- drains central Wake County, including the southern areas of the cities of Raleigh and eastern Cary; no NPDES permitted dischargers in the
watershed; second greatest percentage of its watershed developed than any other Piedmont watershed in 2015; site is - 3.2 miles below the site on Walnut
Creek at South State Street; tributary to Neuse River. Habitats -- low quality instream and riparian habitats, has ranged from 54 to 71; deep, blow-out pools
noted as in 2010; eroding banks; coarse woody debris across the channel and submerged; exotic vegetation (Oriental Privet and Cat Brier) in the riparian
zones; riffles created by large boulders; fish absent in the channel pools; difficult to sample due to hydrologically altered habitats. Water Quality -- specific
conductance elevated due to nonpoint and stormwater runoff from a highly urbanized and impervious watershed, since 1995 has ranged from 139 pS/cm to 208
pS/cm with measurements greater than 200 pS/cm during the past two cycles. 2015 -- fewest fish (n = 130) ever collected and second fewest number of
species (n = 12) collected at any site in the Piedmont in 2015; very high Percentage of Tolerant Fish (67%, highest percentage ever, primarily Redbreast
Sunfish, Green Sunfish, Satinfin Shiner, and Eastern Mosquitofish); suckers absent; an 8-point decrease in the NCIBI Score and a decline in two NCIBI Rating
classes were attributed to the lower than expected metric values for total species richness (including number of species of darters, sunfish, suckers, and
Intolerant Species), number of fish collected, and a high Percentage of Tolerant Fish; lowest NCIBI Score and NCIBI Rating (along with Ellerbe Creek) of any
site in the Neuse Piedmont in 2015. 1991-2015 -- despite its urban setting, a very diverse community of 31 species are known from this site, including 9
species of cyprinids, 7 species of sunfish, 4 species of darters, 3 intolerant species, but just 1 species of sucker; dominant species are Eastern Mosquitofish
(1991), Satinfin Shiner (1995 and 2010), Swallowtail Shiner (2000-2010), and Redbreast Sunfish (2015); since 1995 the community has been characterized as
having a very skewed trophic structure with a rarity of omnivorous Bluehead Chub and Spottail Shiner; lowest NCIBI Score and Rating since 1995.
Recommendation -- as done in 2015, site should continue to be sampled at flows lower than those experienced in 2010 because of the difficulty in sampling
the deep blowout pools and runs.
Most Abundant Redbreast Sunfish (36, Non -indigenous Green Sunfish (11) and Imperiled
Species 28%) Species Bluegill (2) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
�_APPLETREE SWP
SR 1216
JF44
05/28/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
GREENE
03020203
35.506189
-77.775690
55
No
Level IV Ecoregion
Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Rolling Coastal Plain
9.0
1 3
0.4
Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
18.9
0.7
no data
51.3
no data
29.0
0.2
0.0
2001
16.9
3.7
0.2
53.5
9.0
16.4
0.4
0.0
2006
15.6
3.7
0.2
54.7
10.2
16.0
0.4
0.0
Water Quality Parameters
2005
2015
Temperature (°C)
---
18.0
22.7
Dissolved Oxygen (mg/L)
---
6.8
6.7
Specific Conductance (pS/cm)
---
92
106
pH (s.u.)
I ---
6.5
1 5.9
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
10
Instream Habitat (20)
15
Bottom Substrate (15)
14
Pool Variety (10)
8
Bank Erosion (10)
5
Bank Vegetation (10)
7
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
3
Total Habitat Score (100)
77
Water Clarity Clear/tannic
Substrate: Gray sand, gravel, cobble
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
05/28/15
2015-38
22
---
Not Rated
04/22/05
2005-33
19
---
Not Rated
Data
Watershed -- drains northwestern Greene and northeastern Wayne counties; no municipalities or NPDES facilities in this priamrily agriculutral watershed; least
percentage of its watershed in imperviousness cover than any other Neuse Coastal Plain watershed (along with Island Creek) in 2015; tributary to Nahunta
Swamp, site is - 0.4 mile above the stream's confluence with the swamp. Habitats -- moderate quality habitats, score was 82 in 2005; a small, narrow stream
which was channelized a long time ago, one bend in the channel; root mats; coarse woody debris and snags; moderate bank erosion; narrow riparian zone on
the right. Water Quality -- specific conductance was within the norm for a Coastal Plain stream, was 92 pS/cm in 2005.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- 8 species of sunfish collected. 2005 & 2015 -- 27 species known from the site including 9 species of sunfish, 5 species of cyprinids, 3 species each of
catfish and darters, and 1 species of sucker; one of only a few Coastal Plain sites from which the White Shiner was collected which is an obligate colonial
spawner on Bluehead Chub and Bull Chub nests, both species are unknown from this site which may explain the shiner's rarity, and only six specimens of
White Shiner have been collected at this site (in 2005); dominant species is the Dusky Shiner; number of fish collected during both cycles was fairly constant (n
= 221 and 258).
Most Abundant Dusky Shiner (80, 31 %) Non -indigenous Green Sunfish (7) and Imperiled None
Species Species Bluegill (10) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
BEAR CR
SR 1311
JF36
08/17/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
LEN OI R
03020202
35.248889
-77.784444
45
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
F---Southeastern Floodplains & Low Terraces
61.7
8
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
20.1
4.1
no data
61.1
no data
14.0
0.7
0.0
2001
13.2
8.4
1.1
55.9
8.3
13.0
0.8
0.0
2006
12.4
8.5
1.1
56.0
9.8
12.5
0.8
0.0
Water Quality Parameters
2000
2005
2015
Temperature (°C)
23.9
14.6
21.9
Dissolved Oxygen (mg/L)
5.0
8.9
7.4
Specific Conductance (pS/cm)
96
103
110
pH (s.u.)
6.1
6.5
6.2
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
15
Bottom Substrate (15)
7
Pool Variety (10)
10
Bank Erosion (10)
6
Bank Vegetation (10)
8
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
81
Water Clarity I Clear/slightly tannic
Site Photograph
Substrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/17/15
2015-75
18
---
Not Rated
05/04/05
2005-44
23
---
Not Rated
06/14/00
2000-44
22
---
Not Rated
10/28/96
96-74
12
---
Not Rated
05/22/95
95-47
23
Not Rated
Data
Watershed -- drains northwestern Lenoir County, west of the Town of LaGrange, and portions of eastern Wayne and southwestern Greene counties; no
NPDES dischargers in the watershed; tributary to the Neuse River, site is - 2.5 miles upstream from the creek's confluence with the river. Habitats -- high
quality habitats, scores have ranged from 81 in 2000 and 2015 to 89 in 1996 (habitat data were not recorded in 1995); natural channel; undercuts; coarse
woody debris snags, deadfalls, and obstructions; dense stands of Oriental Privet and Cat Brier growing in the riparian zones; east riparian zone has re -
vegetated since being logged in 2005. Water Quality -- low flow; specific conductance was within the norm for a Coastal Plain stream, has ranged from 89
pS/cm in 1995 to 110 pS/cm.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- 4 species of darters collected, but only 3 species of sunfish. 1995-2015 -- an extremely diverse community with 39 species known from the site
including 11 species of sunfish, 8 species of cyprinids, 4 species of darters, and 3 species each of catfish and suckers; dominant species are the tolerant
Satinfin Shiner (1995, 2000, 2005, and and 2015), the tolerant Eastern Mosquitofish (1996), and the intermediate Eastern Silvery Minnow (2015); one specim
each of the Significantly Rare and intolerant Mimic Shiner and of the State Threatened and intolerant Least Brook Lamprey were collected in 1995 and 1996,
respectively; except for 1996 (sampling after hurricane -induced flooding), the number of fish collected has ranged from 223 (2015) to 387 (2000); Percentage
Tolerant Fish has ranged from - 30% to 50%.
Most Abundant o Non -indigenous Bluegill (8) and Channel Imperiled
Species Satinfin Shiner (40, 18/o) Species Catfish (1) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
�__BEAVERDAM CR
SR 1007
JF65
05/27/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
W AYN E
03020201
35.385833
-78.093611
75
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Rolling Coastal Plain
16.3
7
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
26.8
7.4
no data
55.6
no data
10.2
0.1
0.0
2001
18.0
10.8
2.1
51.5
10.1
8.7
0.1
0.0
2006
17.4
11.5
2.2
49.9
11.8
9.3
0.1
0.0
Water Quality Parameters
2005
2015
Temperature (°C)
---
17.9
24.0
Dissolved Oxygen (mg/L)
---
8.6
5.4
Specific Conductance (pS/cm)
---
98
110
pH (s.u.)
I ---
7.1
6.3
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
15
Bottom Substrate (15)
13
Pool Variety (10)
10
Bank Erosion (10)
10
Bank Vegetation (10)
10
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
93
Water Clarity ISlightly turbid/tannic, became very turbid
Substrate: Sand, silt
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
05/27/15
2015-37
25
---
Not Rated
05/03/05
2005-43
22
---
Not Rated
Data Analysis
Watershed -- drains western Wayne and eastern Johnston counties, including the Town of Princeton; no NPDES dischargers in the watershed; tributaryto the
Neuse River, site is - 0.2 mile upstream from the creek's confluence with the river; the locale appeared to have once been an old mill site (concrete vein with
boulders and cobble strewn about). Habitat -- highest quality habitats of any of the sites in the Neuse Coastal Plain in 2015, total score was "80" in 2005;
natural channel; cypress forest bottomlands; deep blow-out pool behind dam remnants; submerged coarse woody debris, snags, and deadfalls. Water Quality
- water became very turbid and did not clear during sampling which was caused by legacy deposits of sediments behind the old dam; specific conductance was
within the norm for a Coastal Plain stream, was 98 pS/cm in 2005; dissolved oxygen only at 64% of saturation.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- only site were the diminutive and uncommonly collected Banded Pygmy Sunfish was encountered (n = 1); 8 species of sunfish and 3 species of suckers
were collected (more species of suckers than any other site from any other year, except for Bear Creek in 1995); as in 2005 this stream supported a large
biomass of large specimens of Bowfin, Redbreast, Bluegill, Largemouth Bass, Chain Pickerel, Redfin Pickerel, Notchlip Redhorse, and Shorthead Redhorse;
Bluespotted Sunfish were extremely abundant. 2005 & 2015 -- a speciose community with 30 species known from the site including 10 species of sunfish, 3
species each of cyprinids, darters, and suckers, and 2 species of catfish; dominant species are the tolerant Eastern Mosquitofish (2005) and Bluespotted
Sunfish (2015); an extremely abundant community (n = 511 in 2005 and 597 in 2015). The creek's close proximity to the Neuse River, good instream and
riparian habitat diversity, and an abundance of prey items may explain the creek's high species diversity and the large sizes of many of the predatory species.
Bluegill
Most Abundant Bluespotted Sunfish (254, Non -indigenous Green Sunfish (5), Imperiled
Species 43%) Species (18), Redear Sunfish (1), and Species None
Channel Catfish (1)

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
�__CLAYROOT SWP
SR 1941
JF68
08/19/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
PITT
03020202
35.385000
-77.247665
15
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Mid -Atlantic Flatwoods
46.1
5
0.2
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
32.6
1.3
no data
28.5
no data
32.6
0.0
5.0
2001
27.4
5.1
0.5
30.4
14.9
22.6
0.1
0.0
2006
24.4
5.1
0.5
31.1
18.3
21.0
0.1
0.0
Water Quality Parameters
1995
2000
2015
Temperature (°C)
27.0
24.6
24.8
Dissolved Oxygen (mg/L)
10.4
4.7
8.5
Specific Conductance (pS/cm)
211
276
286
pH (s.u.)
1 7.6
7.5
1 7.3
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
5
Instream Habitat (20)
7
Bottom Substrate (15)
7
Pool Variety (10)
4
Bank Erosion (10)
6
Bank Vegetation (10)
5
Light Penetration (10)
2
Left Riparian Score (5)
3
Right Riparian Score (5)
5
Total Habitat Score (100)
44
Water Clarity Clear; not tannic
Substrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/19/15
2015-83
22
---
Not Rated
06/13/00
2000-41
23
---
Not Rated
05/22/95
95-50
20
---
Not Rated
06/26/91
91-17
27
---
Not Rated
Data Analysis
Watershed -- drains southeastern Pitt County; no municipalities or NPDES facilities within the creek's rural residential and agricultural watershed; tributaryto
Swift Creek. Habitat -- low quality habitats, second lowest quality habitats of any of the sites in the Neuse Coastal Plain in 2015, scores ranged from 44 in 201E
to 49 in 2000 (data were not collected in 1991); channelized and is maintained as such; deeply entrenched and detached from its flood plain except for during
extreme flow events; de -snagged; minimal shading with an open canopy; uniform depth with very shallow, sandy runs; Egeria and thick growths of periphyton;
grasses overhanging into the water; very shallow undercuts along the banks; narrow riparian zone along the left bank with a maintenance road. Water Quality
- greatest specific conductance of any site in the Coastal Plain in 2015, has ranged from 211 pS/cm in 1995 to 286 pS/cm in 2015 (no data were collected in
1991).
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
5 -- most fish (n = 1,705) ever collected from any Neuse Coastal Plain site in 2015 or from any other years. 1991-2015 -- a speciose community with 32
cies known from the site including 10 species of sunfish, 7 species of cyprinids, 6 species of catfish, 2 species of darters, and 1 species of suckers;
iinant species are the tolerant Redbreast Sunfish (1991), the intermediate American Eel (1995 and 2000) and the intermediate Tessellated Darter and
rant Eastern Mosquitofish (2015); an extremely abundant community (n = 614, 486, 815, and 1,705, respectively since 1991); most species (n = 27) ever
acted from a Neuse Coastal Plain stream was in 1991.
Tessellated Darter (488,
Most Abundant 29%) and Eastern Non -indigenous Green Sunfish (29), Bluegill Imperiled None
Species 29%) andMosquitofE tern 24%) Species (21), and Channel Catfish (2) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
FALLING CR
SR 1340
JF37
08/17/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
LEN OI R
03020202
35.254258
-77.673141
40
No
Level IV Ecoregion
I Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Southeastern Floodplains & Low Terraces
1 50.9
1 5
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
24.0
4.0
no data
55.6
no data
16.2
0.2
0.0
2001
17.5
8.9
1.2
49.0
9.3
14.8
0.2
0.0
2006
17.4
9.0
1.3
48.6
9.9
14.8
0.3
0.1
Water Quality Parameters
2000
2005
2015
Temperature (°C)
24.7
16.1
23.6
Dissolved Oxygen (mg/L)
5.4
8.5
7.2
Specific Conductance (pS/cm)
94
97
107
pH (s.u.)
6.6
7.0
6.3
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
15
Bottom Substrate (15)
7
Pool Variety (10)
10
Bank Erosion (10)
6
Bank Vegetation (10)
8
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
81
Site Photograph
Water Clarity Clear/tannic, but not dark I iSubstrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/17/15
2015-76
26
---
Not Rated
05/04/05
2005-45
26
---
Not Rated
06/14/00
2000-43
25
---
Not Rated
05/22/95
95-48
22
---
Not Rated
Data
Watershed -- drains the Town of LaGrange and the central portion of Lenoir County; a small portion of southern Green County is also within its watershed; one
NPDES facility (NC0021644, Qw = 0.75 to UT Moseley Creek, a tributary to Falling Creek) in the watershed and is located - 10.5 miles upstream; tributary to
the Neuse River, site is - 2.3 miles upstream from the creek's confluence with the river. Habitats -- high to very high quality habitats; scores have ranged from
81 in 2015 to 95 in 2000; natural channel; since 2005, the right riparian zone appears to have been logged and re -vegetated with Oriental Privet, remainde of
the riparian zones are cypress forest bottomlands; open canopy in places with growths of aquatic macrophytes. Water Quality -- low flow; specific conductance
was within the norm for a Neuse Coastal Plain stream, has ranged from 80 pS/cm in 1995 to 107 pS/cm in 2015.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- most species collected from any Neuse Coastal Plain site (along with Nahunta Swamp) in 2015, including four species of darters; Glassy Darter and
Tadpole Madtom were collected for the first time at this site in 2015. 1995-2015 -- an extremely speciose community with 37 species known from the site
including 11 species of sunfish, 7 species of cyprinids, 5 species of darters, 4 species of darters, and 3 species each of catfish and suckers; dominant species
are the American Eel (1995, 2000, and 2015) and the Dusky Shiner (1995 and 2005); except for 1995 (n = 142), the number of fish collected has ranged from
607 in 2015 to 967 in 2005; Percentage of Tolerant Fish has ranged from 15% to 30%.
Most Abundant American Eel (186, 31%) Non -indigenous Green Sunfish=Lj
Imperiled None
Species Species Bluegill (34) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
FORT RUN
NC 58
JF45
08/20/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
GREENE
03020203
35.493611
-77.730278
50
No
Level IV Ecoregion
Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Rolling Coastal Plain
9.8
1 5
0.4
Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
20.7
0.7
no data
60.1
no data
18.4
0.1
0.0
2001
16.4
5.8
0.4
56.8
4.2
16.2
0.4
0.0
2006
14.2
5.8
0.4
56.7
6.6
16.1
0.4
0.2
Water Quality Parameters
2005
2015
Temperature (°C)
---
22.0
25.3
Dissolved Oxygen (mg/L)
---
7.8
3.9
Specific Conductance (pS/cm)
---
82
98
pH (s.u.)
---
6.0
6.0
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
15
Bottom Substrate (15)
1
Pool Variety (10)
10
Bank Erosion (10)
6
Bank Vegetation (10)
6
Light Penetration (10)
7
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
70
Water Clarity I Slightly turbid/tannic
Site Photograph
Substrate: Silt, clay, muck
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/20/15
2015-86
21
---
Not Rated
04/21/05
2005-32
15
---
Not Rated
Data Analysis
Watershed -- drains west -central Greene County; no municipalities or NPDES facilities in the watershed; tributary to Nahunta Creek and ultimately Contentnea
Creek, site is - 0.7 mile upstream from its confluence with Nahunta Swamp. Habitats -- moderately high quality habitats, score was 75" in 2005; natural and
sinuous, but narrow channel; wide riparian zones re -vegetated and overgrown with briars; coarse woody debris (submerged and snags). Water Quality --
specific conductance was within the norm for a Neuse Coastal Plain stream, was 82 pS/cm in 2005; dissolved oxygen was low (< 4 mg/L) and at only 47% of
saturation, but permissible under a supplemental water quality classification of Sw.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2005 & 2015 -- 25 species known from the site including 8 species of sunfish, 4 species of cyprinids, 3 species of catfish, 2 species of darters, and 1 species of
sucker; dominant species are the tolerant Golden Shiner in 2005 and the tolerant Redbreast Sunfish, the intermediate American Eel, and the intermediate
Dusky Shiner in 2015.
Redbreast Sunfish (23,
Most Abundant 13%), American Eel (22, Non -indigenous Green Sunfish (6) and Imperiled
None
Species 12%), and Dusky Shiner Species Bluegill (15) Species
(22, 12%)

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
HOMINY SWP
SR 1606
JF46
04/30/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
W ILSON
03020203
35.692277
-77.905827
85
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Rolling Coastal Plain
9.8
6
0.6
Upstream NPDES Dischargers (? 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
27.4
49.7
no data
15.6
no data
7.2
0.0
0.0
2001
10.6
71.3
21.0
8.5
5.5
2.9
0.1
0.0
2006
8.5
75.4
22.5
6.7
6.3
2.7
0.1
0.5
Water Quality Parameters
1995
2005
2015
Temperature (°C)
26.0
16.8
15.8
Dissolved Oxygen (mg/L)
3.2
3.7
5.3
Specific Conductance (pS/cm)
242
196
250
pH (s.u.)
no data
6.9
6.1
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
10
Instream Habitat (20)
6
Bottom Substrate (15)
4
Pool Variety (10)
10
Bank Erosion (10)
1
Bank Vegetation (10)
5
Light Penetration (10)
2
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
48
Water Clarity ITurbid
Substrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/30/15
2015-24
9
---
Not Rated
05/02/05
2005-40
10
---
Not Rated
08/03/95
95-75
19
---
Not Rated
Data
Watershed -- drains the City of Wilson in central Wilson County; greatest percentage of its watershed developed and least forested and least in
grassland/herbaceous/shrubland than any other Neuse Coastal Plain watershed in 2015; no NPDES facilities in the watershed; tributary to Contentnea Creek,
site was - 3.5 miles upstream from the swamp's confluence with the river. Habitats -- low quality habitats; channelized a long time ago; this site received the
lowest habitat score of any non- recently maintained channelized fish community site in the Neuse Coastal Plain in 2005 and in 2015, scores have ranged from
46 in 2005 to 53 in 1995; severe bank erosion, fairly open canopy; stream and stream bank choked with urban debris and trash. Water Quality -- sewage
smell; conductivity elevated indicative of nonpoint source urban runoff and leaky sewer lines; second greatest specific conductance of any site in the Neuse
Coastal Plain in 2015, has ranged from 196 pS/cm to 250 pS/cm; dissolved oxygen only at 53% of saturation, but permissible under a supplemental water
ouality classification of Sw.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- fewest species collected and second greatest Percentage of Tolerant Fish (63%) from any Neuse Coastal Plain site in 2015, only 3 species of sunfish
collected and darters were absent; 1 of only 2 sites where the wide -spread American Eel was not collected and in fact has never been collected at this site.
1995-2015 -- 23 species known from the site including 9 species of sunfish, 5 species of cyprinids, 2 species of catfish, and 1 species each of darter and
sucker; 1 of only 7 Neuse Coastal Plain sites from which the White Shiner was collected and which is is an obligate colonial spawner on Bluehead Chub and
Bull Chub nests, both chub species are unknown from the site which and along with degraded water quality, may explain the shiner's rarity at this site, the White
Shiner has not been collected from this site since 1995; dominant species is the tolerant Eastern Mosquitofish; Percentage of Tolerant Fish has ranged from -
Most Abundant Eastern Mosquitofish Non -indigenous ( glue ill 15) Imperiled None
Species (110, 62%) Species g Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
�_INDIAN WELL SWP
SR 1753
JF56
08/20/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
PITT
03020202
35.459444
-77.283889
35
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Mid -Atlantic Flatwoods
14.8
4
0.4
Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
28.5
2.7
no data
38.3
no data
30.6
0.0
0.0
2001
21.3
6.6
0.8
44.7
7.3
20.0
0.1
0.0
2006
19.1
6.6
0.8
44.9
10.2
19.0
0.1
0.1
Water Quality Parameters
2005
2015
Temperature (°C)
---
18.1
24.2
Dissolved Oxygen (mg/L)
---
8.1
3.8
Specific Conductance (pS/cm)
---
147
148
pH (s.u.)
I ---
6.7
6.4
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
5
Instream Habitat (20)
7
Bottom Substrate (15)
4
Pool Variety (10)
6
Bank Erosion (10)
6
Bank Vegetation (10)
5
Light Penetration (10)
2
Left Riparian Score (5)
2
Right Riparian Score (5)
5
Total Habitat Score (100)
42
Site Photograph
Water Clarity Slightly turbid/not tannic; easily silted by gray clay and silts, did not clear Substrate: Sand, silt, clay
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/20/15
2015-84
20
---
1
Not Rated
05/18/05
2005-57
23
---
Not Rated
Data
Watershed -- drains southeastern Pitt County; no municipalities or NPDES facilities in the watershed; tributary to Clayroot Swamp. Habitat -- lowest quality
habitats of any of the sites in the Neuse Coastal Plain in 2015, was scored a "53" in 2005; channelized and is maintained as such; deeply entrenched and
detached from its flood plain except for during extreme flow events; de -snagged; minimal shading with an open canopy; uniform depth; Egeria; grasses
overhanging into the water; shallow undercuts along the banks; farm path on the left bank for mowing and snag removal from stream, then forested. Water
Quality -- specific conductance elevated, was 147 pS/cm in 2005; dissolved oxygen was low (< 4 mg/L) and at only 45% of saturation, but permissible under a
supplemental water quality classification of Sw.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- large specimens of Largemouth Bass, Bluegill, and Chain Pickerel for such as small stream; the greatest Percentage of Diseased Fish (3.54%, Redsore
Disease in Bluegill and Redbreast Sunfish) from any site for any year in the Neuse Coastal Plain. 2005 & 2015 -- 27 species known from the site including 8
species of sunfish, 5 species of cyprinids, 4 species of catfish, and 1 species each of darters and suckers; dominant species are the tolerant Redbreast Sunfish
(2005) and the intermediate Bluegill (2015); number of fish collected during the past two cycles has been stable (218 and 198).
Most Abundant ° Non -indigenous Imperiled
Species Bluegill (59, 30 /o) Species Bluegill (59) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
ISLAND CR
SR 1004
JF60
08/18/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
JONES
03020204
35.016828
-77.134460
15
No
Level IV Ecoregion
Drainage Area (mi)
Stream Width (m)
Stream Depth (m)
Carolina Flatwoods
8.9
4
1 0.5
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
51.4
0.0
no data
1.8
no data
42.0
0.0
4.7
2001
45.7
2.9
0.2
0.2
10.3
40.9
0.0
0.0
2006
36.8
2.9
0.2
3.0
16.9
40.3
0.0
0.0
Water Quality Parameters
2000
2005
2015
Temperature (°C)
22.4
19.3
24.3
Dissolved Oxygen (mg/L)
4.3
5.6
5.2
Specific Conductance (pS/cm)
270
99
127
pH (s.u.)
1 7.1
6.7
1 6.4
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
10
Bottom Substrate (15)
4
Pool Variety (10)
10
Bank Erosion (10)
8
Bank Vegetation (10)
10
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
77
Water Clarity Clear/tannic, very dark
Substrate: Sand, silt, muck
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/18/15
2015-80
11
---
Not Rated
05/17/05
2005-54
10
---
Not Rated
06/12/00
2000-38
15
---
Not Rated
08/15/95
1 95-78
1 15
1
1 Not Rated
03/23/95
1 95-09
1 8
1 ---
I Not Rated
Data An
Watershed -- drains the extreme northeast corner of Jones County, including a portion of the Croatan National Forest; greatest percentage of its watershed in
wetland and least developed, in impervious, and in cultivation cover than any other Neuse Coastal Plain watershed in 2015; no municipalities or NPDES
dischargers in the watershed; tributary to the Trent River. Habitats -- moderate quality habitats, scores were 87 in 2000 and 90 in 2005 (data were not collected
in 1995); very sinuous natural channel flowing between limestone hills; dense shade offered by the wide riparian zones; coarse woody debris (submerged and
snags); Fissidens. Water Quality -- low flow; the specific conductance has been elevated at times -- in August 1995 it was 217 pS/cm, in 2000 it was 270
pS/cm, but in March 1995 it was 105 pS/cm, in 2005 it was 99 pS/cm, and in 2015 it was 127 pS/cm; in 2015, it was determined that the elevated readings
originate from an unnamed tributary on the left (the reading was 317 pS/cm and the pH was 7.2 s.u.) that drains a unique geological (soil) formation or transports
the backwash from a saltwater swimming pool; like other streams in this subbasin, the pH was not low because of the effects of the limestone geology; the water
is extremely dark and tannin stained.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- second lowest Percentage of Tolerant Fish (10%) from any Neuse Coastal Plain site in 2015; large specimens of Pumpkinseed, Redbreast Sunfish,
Warmouth, and Bluegill; fewest species of any Neuse Coastal Plain site (along with Watery Branch). 1995-2015 -- fewest species of any Neuse Coastal Plain
site (along with Watery Branch) only 19 species known from the site including 8 species of sunfish, 2 species each of catfish and darters, and just 1 species
each of cyprinids and suckers; dominant species are the Pumpkinseed (May 1995, 2005, and 2015), the American Eel (August 1995 and 2000), and the Dusky
Shiner (2005); except for in 2000 (n = 206), the number of fish collected has been very low (n = 34-80); Percentage of Tolerant Fish has been <_ 10%; fewest
species (8) ever collected from a Coastal Plain stream was in March 1995; most fewest fish ever collected from a Coastal Plain stream was in 1995 (43 in March
and 34 in August).
American Eel and
Most Abundant Pumpkinseed Sunfish (22 Non -indigenous ed
Bluegill (7) ImperilNone
Species each, 30%) Species Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
LITTLE BUFFALO CR
SR 2143
JF42
04/29/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
JOHNSTON
03020201
35.596441
-78.163427
135
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Rolling Coastal Plain
23.7
7
1 0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
44.6
1.6
no data
40.3
no data
12.9
0.1
0.5
2001
27.0
8.4
1.3
39.6
10.4
13.2
0.1
0.0
2006
26.0
8.4
1.3
39.5
13.0
12.9
0.1
0.0
Water Quality Parameters
2005
2015
Temperature (°C)
---
15.3
14.4
Dissolved Oxygen (mg/L)
---
7.3
8.4
Specific Conductance (pS/cm)
---
65
71
pH (s.u.)
---
6A
5.4
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
15
Bottom Substrate (15)
14
Pool Variety (10)
10
Bank Erosion (10)
6
Bank Vegetation (10)
7
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
87
Water Clarity Clear/Tannic
Site Photograph
Substrate: Sand, white gravel
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/29/15
2015-20
21
---
Not Rated
04/19/05
2005-27
16
---
Not Rated
Data
Watershed -- drains northeastern Johnston County, northwest of the Town of Kenly, and also a portion of southwest Wilson County; no municipalities or
NPDES dischargers in the watershed; tributary to the Little River, site is - 1.1 miles upstream from the creek's confluence with the river. Habitat -- second
highest quality habitats of any of the sites in the Neuse Coastal Plain in 2015; scored an "87" in both cycles; natural channel; wide riparian zones; coarse woody
debris and deadfalls. Water Quality -- second lowest specific conductance of any site in the Neuse Coastal Plain in 2015, was also low in 2005 at 65 pS/cm.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
5 -- one of only a few Coastal Plain sites from which the White Shiner and only Coastal Plain site from which the intolerant Pinewoods Shiner were
acted, both species are obligate colonial spawners on Bluehead Chub and Bull Chub nests, Bluehead Chub is unknown from the site, and only one
cimen of Bull Chub has been collected at this site (in 2005) which explains the Pinewoods Shiner's rarity; 30 specimens of White Shiner were collected in
5 and 2015. 2005 & 2015 -- 26 species known from the site including 8 species of sunfish, 7 species each of cyprinids, 3 species of catfish, 2 species of
:ers, and 1 species of sucker; dominant species are all intermediate species -- Bluegill (2005 and 2015), Redfin Pickerel (2005), and White Shiner (2015).
Most Abundant White Shiner (29, 20%) Non -indigenous Green Sunfish (2) and Imperiled None
Species Species Bluegill (26) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
LITTLE CONTENTNEA CR
SR 2107
JF47
08/20/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
F____PlTT
03020203
35.611389
-77.616667
60
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Rolling Coastal Plain
34.8
6
1 0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
25.2
1.5
no data
40.7
no data
31.7
0.2
0.7
2001
23.9
6.3
0.6
40.0
9.0
20.0
0.6
0.0
2006
22.0
6.3
0.6
40.2
11.8
19.1
0.7
0.0
Water Quality Parameters
1995
2005
2015
Temperature (°C)
17.5
19.6
23.7
Dissolved Oxygen (mg/L)
5.1
5.2
0.9
Specific Conductance (pS/cm)
99
95
121
pH (s.u.)
I no data
1 6.3
1 6.0
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
10
Instream Habitat (20)
10
Bottom Substrate (15)
1
Pool Variety (10)
10
Bank Erosion (10)
6
Bank Vegetation (10)
8
Light Penetration (10)
9
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
64
Water Clarity ITurbid, easily silted, not tannic
Substrate: Soft clay, silt, and muck
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/20/15
2015-85
20
---
Not Rated
05/18/05
2005-58
19
---
Not Rated
05/23/95
95-52
12
---
Not Rated
Data Analysis
Watershed -- drains the southwestern corner of Pitt County, north of the Town of Farmville and also portions of northeastern Wilson and northern Green
counties; no municipalities or NPDES facilities within the watershed; tributary to Contentnea Creek. Habitats -- moderate quality habitats; scores have ranged
from 54 in 1995 to 80 in 2005; channelized a long time ago, the wide riparian forests have re -vegetated since then, but with dense growths of Oriental Privet;
deep, mid -channel pools and runs and coarse woody debris (submerged and snags). Water Quality -- specific conductance was within the norm for a Neuse
Coastal Plain stream, has ranged from 95 pS/cm in 2005 to 121 pS/cm in 2015; dissolved oxygen was extremely low (< 1 mg/L) and at only 11 % of saturation,
but permissible under a supplemental water quality classification of Sw.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- most species ever collected at this site; most fish (n = 341 vs. 34 in 1995 and 119 in 2005) ever collected at this site; very large and abundant
specimens of Chain Pickerel (203-595 mm TL), Creek Chubsucker, Redfin Pickerel, Flier, Largemouth Bass, Bluegill, and Golden Shiner for such as small
stream. 1995-2015 -- 26 species known from the site including 9 species of sunfish, 4 species of cyprinids, 3 species of catfish, 2 species of darters, and 1
species of sucker; dominant species is the intermediate Bluegill.
Most Abundant Bluegill (162, 48%) Non -indigenous Bluegill (162) Imperiled None
Species Species Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
MILL RUN
NC 58
JF62
08/19/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
J ON E S
03020204
35.005833
-77.281389
5
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Carolina Flatwoods
12.7
6
1 0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
39.0
0.0
no data
28.1
no data
25.7
0.0
7.3
2001
35.5
4.2
0.4
26.3
24.9
8.9
0.1
0.0
2006
30.1
4.2
0.4
33.8
22.3
9.2
0.3
0.0
Water Quality Parameters
2000
2005
2015
Temperature (°C)
22.8
19.3
22.7
Dissolved Oxygen (mg/L)
4.8
6.2
6.7
Specific Conductance (pS/cm)
212
169
130
pH (s.u.)
7.1
6.5
6.1
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
12
Bottom Substrate (15)
3
Pool Variety (10)
10
Bank Erosion (10)
8
Bank Vegetation (10)
9
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
77
Water Clarity I Slightly turbid/tannic, dark
Substrate: Sand, silt, muck
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/19/15
2015-81
17
---
Not Rated
05/17/05
2005-53
16
---
Not Rated
06/12/00
2000-39
19
---
Not Rated
Data
Watershed -- drains east -central Jones County, including the White Oak Pocosin, greatest percentage of its watershed in grassland/herbaceous/shrub than
any other Neuse Coastal Plain watershed in 2015; no municipalities or NPDES dischargers in the watershed; tributary to the Trent River, site is - 0.4 mile
upstream from the streams' confluence with the river. Habitat -- moderate quality habitats, although scores were greater in 2000 and 2005 (97 and 95,
respectively); natural channel; pools, runs, undercuts, and snags; very densely shaded (by the wide riparian zones (left riparian - bluff, right riparian - Oriental
Privet, Cat Brier, and Bald Cypress); coarse woody debris (submerged and snags) and cypress knees. Water Quality-- specific conductance was within the
norm for a Neuse Coastal Plain stream, but was 212 pS/cm in 2000 and 169 pS/cm in 2005; the pH has also been decreasing since 2000.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- fewest fish ever collected at this site (n = 129 vs. 345 in 2000 and 303 in 2005); large specimens of the piscivorous Chain Pickerel, Redfin Pickerel, and
Largemouth Bass. 2000-2015 -- 24 species known from the site including 8 species of sunfish, 4 species of cyprinids, 2 species each of catfish and darters,
and 1 species of sucker; dominant species are the intermediate American Eel (2000 and 2015) and the intermediate Dusky Shiner (2005 and 2015);
Percentage of Tolerant Fish ranges from - 15% to 25%.
Most Abundant American Eel (27, 21%) Non-indBlueill igenous g (2) Imperiled None
Species Species Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
MOSLEY CR
SR 1475
JF38
08/17/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
CRAVEN
03020202
35.329877
-77.426656
15
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Mid -Atlantic Floodplains & Low Terraces
45.7
1 9
0.3
Upstream NPDES Dischargers (z 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
37.3
1.0
no data
30.3
no data
29.6
0.1
1.7
2001
35.1
4.9
0.5
26.4
12.3
21.1
0.1
0.0
2006
31.1
4.9
0.5
25.9
18.0
19.9
0.1
0.0
Water Quality Parameters
2000
2005
2015
Temperature (°C)
25.1
21.9
26.6
Dissolved Oxygen (mg/L)
5.4
6.3
8.4
Specific Conductance (pS/cm)
188
154
200
pH (s.u.)
7.3
6.5
6.9
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
10
Instream Habitat (20)
8
Bottom Substrate (15)
7
Pool Variety (10)
6
Bank Erosion (10)
3
Bank Vegetation (10)
5
Light Penetration (10)
2
Left Riparian Score (5)
4
Right Riparian Score (5)
5
Total Habitat Score (100)
50
Water Clarity I Clear/slightly tannic, easily silted by clays
Substrate: Soft, shifting sand, bank clay
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/17/15
2015-77
23
---
Not Rated
05/17/05
2005-55
25
---
Not Rated
06/13/00
2000-42
25
---
Not Rated
10/29/96
96-77
16
---
Not Rated
04/19/95
F--06/27/91
95-28
15
Not Rated
91-16
21
Not Rated
Data
Watershed -- drains northwestern Jones, northeastern Lenoir, and western Craven counties; upper watershed is transected by the US 70 corridor; no
municipalities or NPDES dischargers in the watershed; tributary to the Neuse River, site is - 2.3 miles upstream from the creek's confluence with the river.
Habitats -- low quality habitats, scores have ranged from 39 in 1996 (following Hurricane Fran) to 64 in 2005; channelization continues to be maintained, but
with some bends in the channel; deeply entrenched; de -snagged; minimal shading with an open canopy; shallow, sandy runs; one deep clay -bottomed pool.
Water Quality -- low flow; specific conductance consistently elevated, has ranged from 154 pS/cm in 2005 to 200 pS/cm in 1995 and 2015.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- Percentage of Tolerant Fish was the lowest ever observed at this site (13%); the greatest percentage of Omnivores+Herbivores (45%, primarily Eastern
Silvery Minnow) from any Neuse Coastal Plain site from any year. 1991-2015 -- a speciose community with 33 species known from the site including 11
species of sunfish, 7 species of cyprinids, 4 species of catfish, 3 species of darters, and 1 species of sucker; dominant species are the tolerant Redbreast
Sunfish (1991, 2000, and 2005), the intermediate Eastern Silvery Minnow (1995 and 2015), and the tolerant Eastern Mosquitofish (1996); except for 2015, the
Percentage of Tolerant Fish has ranged from - 30% to 50%.
Most Abundant Eastern Silvery Minnow Non -indigenous Green Sunfish (9), Bluegill Imperiled
None
Species 1(286,45%) Species 1(27), and Channel Catfish (2) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
�_NAHUNTASWP
SR 1537
JF49
04/30/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
WAYNE
03020203
35.511856
-77.948397
90
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Rolling Coastal Plain
24.9
7
0.5
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
24.9
1.9
no data
56.3
no data
16.8
0.1
0.0
2001
14.3
6.6
0.8
56.3
6.9
17.8
0.1
0.0
2006
15.1
6.9
0.9
55.6
7.8
14.4
0.1
0.0
Water Quality Parameters
2005
2015
Temperature (°C)
---
17.0
14.6
Dissolved Oxygen (mg/L)
---
6.9
8.7
Specific Conductance (pS/cm)
---
96
109
pH (s.u.)
---
6.3
5.7
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
10
Instream Habitat (20)
12
Bottom Substrate (15)
4
Pool Variety (10)
8
Bank Erosion (10)
5
Bank Vegetation (10)
7
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
4
Total Habitat Score (100)
65
Site Photograph
Water Clarity I Clear/tannic Substrate: Sand, clay
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/30/15
2015-25
26
---
Not Rated
04/20/05
2005-28
22
---
Not Rated
Data Analysis
Watershed -- drains northern Wayne and northeastern Johnston counties, including the towns of Fairmont and Pineville; no NPDES dischargers in the
watershed; tributary to Contentnea Creek. Habitats -- moderate quality habitats, score was 76 in 2005; channelized a long time ago, but still with a straight
channel; riparian zone overgrown with Oriental Privet; bank erosion moderate. Water Quality -- specific conductance was within the norm for a Neuse Coastal
Plain stream, was 96 pS/cm in 2005.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- most species collected from any Neuse Coastal Plain site (along with Falling Creek) in 2015; 8 species of sunfish collected. 2005 & 2015 -- a speciose
community with 31 species known from the site including 10 species of sunfish, 7 species of cyprinids, 3 species each of darters and catfish, and 1 species of
sucker; 1 of only 7 Neuse Coastal Plain sites from which the White Shiner was collected which is is an obligate colonial spawner on Bluehead Chub and Bull
Chub nests; Bluehead Chub is unknown from the site and only one specimen of Bull Chub has been collected at this site (in 2005) which may explain the
shiner's rarity at this site; dominant species are the intermediate Johnny/Tessellated Darter Intergrades (2005) and the intermediate Dusky Shiner (2015);
number of fish has been stable (n = 259 in 2005 and 271 in 2015).
Most Abundant Dusky Shiner (98, 36%) Non -indigenous Green Sunfish (1), Bluegill Imperiled None
Species Species (32), and Redear Sunfish (4) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
RAINBOW CR
SR 1091
JF50
05/28/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
G RE E N E
03020203
35.413025
-77.601504
35
No
Level IV Ecoregion
Drainage Area (mil)
Stream Width (m)
Stream Depth (m)
Rolling Coastal Plain
14.2
6
1 0.5
Upstream NPDES Dischargers (? 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
29.6
1.4
no data
56.1
no data
12.8
0.1
0.0
2001
20.2
6.6
0.6
50.3
3.5
13.7
0.1
0.6
2006
19.6
6.6
0.6
50.5
9.3
13.7
0.3
0.0
Water Quality Parameters
2005
2015
Temperature (°C)
---
15.7
21.9
Dissolved Oxygen (mg/L)
---
7.8
6.6
Specific Conductance (pS/cm)
---
78
102
pH (s.u.)
---
6.4
5.3
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
13
Bottom Substrate (15)
13
Pool Variety (10)
10
Bank Erosion (10)
5
Bank Vegetation (10)
7
Light Penetration (10)
10
Left Riparian Score (5)
3
Right Riparian Score (5)
3
Total Habitat Score (100)
79
Water Clarity I Slightly turbid/tannic
Substrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
05/28/15
2015-40
21
1
---
1
Not Rated
05/05/05
2005-48
17
---
Not Rated
Data
Watershed -- drains southeastern Greene County; no municipalities or NPDES facilities in the watershed; tributary to Contentnea Creek, site is - 1.4 miles
above the stream's confluence with Contentnea Creek. Habitats -- moderate quality habitats, score was 87 in 2005; natural channel; moderate bank erosion;
narrow riparian zones; an abundance of coarse woody debris and deadfalls (submerged and across the channel); poor quality riparian zones (the riparian zone
on the right had been logged in the past and had re -vegetated with thick stands of Oriental Privet, Oriental Bittersweet, and Cat Brier). Water Quality --
specific conductance was within the norm for a Neuse Coastal Plain stream, was lower in 2005 at 78 pS/cm.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
& 2015 -- 27 species known from the site including 9 species of sunfish, 4 species each of cyprinids and darters, 3 species of catfish, and 1 species of
:r; dominant species are the intermediate Dusky Shiner (2005) and the intermediate American Eel (2015).
Green Sunfish (2), Bluegill
Most Abundant American Eel (30, 30%) Non -indigenous (16), Redear Sunfish (1), and Imperiled None
Species Species Channel Catfish (1) Species

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
STONEY CR
SR 1920
JF39
04/29/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
W AYN E
03020202
35.348413
-77.979891
0
No
Level IV Ecoregion
I Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Southeastern Floodplains & Low Terraces
1 27.6
1 6
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
22.1
23.9
no data
40.1
no data
13.3
0.1
0.6
2001
11.0
34.3
9.6
35.3
8.6
10.2
0.1
0.0
2006
10.1
36.6
10.3
34.3
8.6
10.1
0.1
0.2
Water Quality Parameters
2000
2005
2015
Temperature (°C)
18.0
18.1
14.7
Dissolved Oxygen (mg/L)
6.9
6.4
9.1
Specific Conductance (pS/cm)
97
97
117
pH (s.u.)
6.6
6.4
5.6
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
13
Bottom Substrate (15)
7
Pool Variety (10)
8
Bank Erosion (10)
6
Bank Vegetation (10)
8
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
77
Water Clarity Clear/tannic
Substrate: Sand, gravel
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/29/15
2015-21
23
---
Not Rated
04/20/05
2005-29
17
---
Not Rated
04/17/00
2000-15
15
---
Not Rated
07/20/95
95-68
13
---
Not Rated
Data
Watershed -- drains a large portion of the City of Goldsboro as well as Seymour Johnson Air Force Base in central Wayne County; no NPDES dischargers in
the watershed; tributary to the Neuse River, site is - 1.8 miles upstream from the creek's confluence with the river. Habitats -- moderate to high quality habitats
despite draining such a developed watershed, scores ranged from 77 in 2015 to 86 in 2000 and 2005; natural, narrow channel; wide riparian zones; coarse
woody debris and snags; urban debris in the channel and along the banks; Callitriche growing in the sun -lit areas near the bridge. Water Quality -- specific
conductance was within the norm for a Neuse Coastal Plain stream, relatively stable, has ranged from 97 pS/cm in 2000 to 121 pS/cm in 1995.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- most species and sunfish species ever collected at this site including eight species which were new to the site -- Comely Shiner, Redfin Pickerel,
Channel Catfish, Flier, Pumpkinseed, Redear Sunfish, Dollar Sunfish, and Swamp Darter; 8 species of sunfish collected; Percentage of Tolerant Fish was the
greatest ever observed at this site (47%, primarily Redbreast Sunfish and Eastern Mosquitofish). 1991-2015 -- a speciose community with 31 species known
from the site including 9 species of sunfish, 6 species of cyprinids, 4 species each of catfish and darters, and 1 species of sucker (but no wide -spread Creek
Chubsucker); dominant species is consistently the intermediate American Eel; number of fish collected during the past three cycles has been very stable (n =
228-262); except for in 2015, the Percentage of Tolerant Fish has ranged from - 15% to 30%.
Most Abundant Redbreast Sunfish (91, Non -indigenous Bluegill (15), Redear Sunfish Imperiled
Species 40%) Species (1), and Channel Catfish (4) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
SWIFT CR
SR 1753
JF57
08/19/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
k____PlTT
03020202
35.401667
-77.331944
20
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Mid -Atlantic Flatwoods
83.6
13
0.4
Upstream NPDES Dischargers (> 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
22.8
6.3
no data
45.1
no data
24.9
0.5
0.4
2001
15.1
14.6
3.3
43.7
6.2
19.5
0.5
0.0
2006
13.3
17.9
4.4
41.8
7.8
18.4
0.5
0.1
Water Quality Parameters
2005
2015
Temperature (°C)
---
18.5
24.8
Dissolved Oxygen (mg/L)
---
7.6
5.9
Specific Conductance (pS/cm)
---
142
97
pH (s.u.)
I ---
6.5
1 6.5
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
5
Instream Habitat (20)
6
Bottom Substrate (15)
4
Pool Variety (10)
4
Bank Erosion (10)
8
Bank Vegetation (10)
3
Light Penetration (10)
2
Left Riparian Score (5)
5
Right Riparian Score (5)
2
Total Habitat Score (100)
39
Site Photograph
Water Clarity IGray turbidity, not stained Substrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/19/15
2015-82
21
---
1
Not Rated
05/18/05
2005-56
22
---
Not Rated
Data Analysis
Watershed -- largest watershed which was assessed in the Neuse Coastal Plain in 2015; drains the agricultural areas of southeastern and south-central Pitt
County and the suburbs of the southern part of the City of Greenville; one NPDES facility (NC0073229, no permitted limit to its discharge) in the watershed,
located approximately nine miles upstream on an unnamed tributary to Swift Creek; large tributary to the Neuse River. Habitats -- low quality habitats, score
was 42 in 2005; channelized and is maintained as such; deeply entrenched and detached from its flood plain except for during extreme flow events; de -
snagged; minimal shading with an open canopy; uniform depth with shallow, sandy runs; grasses overhanging into the water; shallow undercuts along the
banks; narrow riparian zone along the right bank. Water Quality-- normal flow (bank to bank); specific conductance was within the norm for a Neuse Coastal
Plain stream, was greater in 2005 at 142 pS/cm.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- greatest Percentage of Tolerant Fish (71 %) from any Neuse Coastal Plain site in 2015. 2005 & 2015 -- 26 species known from the site including 9
species of sunfish, 4 species each of cyprinids and catfish, 1 species each of darter and sucker; dominant species are the two tolerant species -- Redbreast
Sunfish (2005) and Eastern Mosquitofish (2015).
Most Abundant Eastern Mosquitofish Non -indigenous Green Sunfish (4), Bluegill Imperiled
Species 1(241,42%) Species (42), and Channel Catfish (3) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
THE SLOUGH
SR 1535
JF51
04/29/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
W AYN E
03020203
35.470531
-77.883450
75
No
Level IV Ecoregion
Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Rolling Coastal Plain
15.9
1 7
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
26.9
4.0
no data
53.9
no data
15.0
0.2
0.0
2001
15.3
8.9
1.2
55.4
5.2
14.4
0.4
0.0
2006
13.4
9.0
1.2
55.7
7.2
14.3
0.4
0.2
Water Quality Parameters
2000
2005
2015
Temperature (°C)
25.0
19.6
15.5
Dissolved Oxygen (mg/L)
4.8
6.1
5.6
Specific Conductance (pS/cm)
92
97
98
pH (s.u.)
6.7
6.2
5.8
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
10
Instream Habitat (20)
12
Bottom Substrate (15)
13
Pool Variety (10)
8
Bank Erosion (10)
6
Bank Vegetation (10)
8
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
77
Site Photograph
Water Clarity Clear/tannic I ISubstrate: Sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/29/15
2015-22
14
---
Not Rated
04/20/05
2005-30
20
---
Not Rated
05/25/00
2000-37
26
---
Not Rated
08/03/95
95-74
14
---
Not Rated
Data
Watershed -- drains north -central Wayne County; no municipalities or NPDES dischargers in the watershed; a small tributary to Nahunta Swamp. Habitats --
moderate quality habitats, has ranged from 69 to 79 since 1995; channelized a long time ago; wide riparian zones offering good overhead canopy; coarse
woody debris and snags in the channel; Oriental Privet in the riparian zones. Water Quality -- specific conductance stable and within the norm for a Neuse
Coastal Plain stream, has ranged from 80 pS/cm in 1995 to 98 pS/cm in 2015.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- fewest fish and species collected since 1995, but Highfin Shiner, Chain Pickerel, and Glassy Darter were collected for the first time at the site; only 3
species of sunfish collected. 1991-2015 -- a speciose community with 32 species known from the site including 9 species of cyprinids, 8 species of sunfish, 5
species of darters, 2 species of catfish, and 1 species of sucker; 1 of only 7 Neuse Coastal Plain sites from which the intermediate White Shiner and the
intolerant Pinewoods Shiner were collected, both species are obligate colonial spawners on Bluehead Chub and Bull Chub nests; Bull Chub is not known from
this site and the other three species are uncommon -- 1 specimen of Bluehead Chub was collected in 2000, 13 specimens of White Shiner were collected
between 1995 and 2005, and 2 specimens of Pinewoods Shiner were collected in 2000; dominant species are the intermediate Dusky Shiner (1995, 2005, and
2015) and the tolerant Redbreast Sunfish (2000); the Percentage of Tolerant Fish has ranged from - 15% to 30%;
Most Abundant o Non -indigenous Imperiled
Species Dusky Shiner (57, 38 /o) Species Bluegill (1) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
TOISNOT SWP
US 264
JF53
05/27/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
W ILSON
1 03020203
35.678029
-77.846041
70
No
Level IV Ecoregion
Drainage Area ( )
Stream Width (m)
Stream Depth
Rolling Coastal Plain
68.0
10
0.4 �E]
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
22.3
6.7
no data
50.0
no data
20.1
0.8
0.0
2001
15.0
14.9
3.4
46.3
6.0
15.7
0.8
0.0
2006
15.1
16.1
3.7
45.2
7.1
15.5
0.9
0.0
Water Quality Parameters
1991
2005
2015
Temperature (°C)
22.5
16.3
22.9
Dissolved Oxygen (mg/L)
5.2
7.3
5.9
Specific Conductance (pS/cm)
100
86
107
pH (s.u.)
no data
6.5
5.8
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
5
Instream Habitat (20)
10
Bottom Substrate (15)
13
Pool Variety (10)
10
Bank Erosion (10)
5
Bank Vegetation (10)
7
Light Penetration (10)
7
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
67
Water Clarity I Slightly turbid/tannic
Site Photograph
Substrate: Sand, silt
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
05/27/15
2015-36
25
---
Not Rated
05/03/05
2005-41
23
---
Not Rated
06/05/91
91-08
22
---
Not Rated
Data
Watershed -- drains southern Nash and north -central Wilson counties, including the eastern edge of the City of Wilson; two NPDES facilities upstream from the
site (NC0037915, Southern Nash High School, - 22 miles upstream, QW = 0.015 MGD and NC0084581, Wilson Technical Community College, - 4.5 miles
upstream, QW = 0.0144 MGD), neither facility (because of its size and distance upstream) should have a significant impact on the aquatic community at the site;
large tributary to Contentnea Creek. Habitats -- moderate quality habitats, score was 89 in 2005; channelized a long time ago, but still with a straight channel;
wide riparian zones; moderate bank erosion. Water Quality -- specific conductance was within the norm for a Neuse Coastal Plain stream, has ranged from 86
pS/cm in 2005 to 107 pS/cm in 2015.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- most species ever collected at this site with Eastern Silvery Minnow, Comely Shiner, White Catfish, and Channel Catfish new to the site; 8 species of
sunfish collected. 1991-2015 -- a speciose community with 33 species known from the site including 10 species of sunfish, 5 species each of cyprinids and
catfish, 4 species of darters, and 1 species of sucker; dominant species are the tolerant Redbreast Sunfish (1991), intermediate Bluegill (2005), and the tolerant
Eastern Mosquitofish (2015).
Bluegill
Most Abundant Eastern Mosquitofish (52, Non -indigenous Green Sunfish (2), Imperiled
Species 21 %) Species (39), Redear Sunfish (4), and Species None
Channel Catfish (1)

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
TRENT R
SR 1130
JF63
08/18/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
JONES
03020204
35.051667
-77.567500
45
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Carolina Flatwoods
48.0
4
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
39.2
0.6
no data
39.0
no data
21.2
0.1
0.0
2001
32.9
3.6
0.3
35.0
11.9
16.0
0.2
0.0
2006
31.3
3.6
0.3
35.9
12.7
16.2
0.2
0.0
Water Quality Parameters
1991
2005
2015
Temperature (°C)
no data
17.4
24.1
Dissolved Oxygen (mg/L)
no data
6.2
5.2
Specific Conductance (pS/cm)
no data
106
119
pH (s.u.)
no data
6.4
6.6
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
12
Bottom Substrate (15)
7
Pool Variety (10)
10
Bank Erosion (10)
6
Bank Vegetation (10)
10
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
80
Site Photograph
Water Clarity I Clear/tannic, easily silted I iSubstrate: Sand, silt, muck
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/18/15
2015-79
21
---
Not Rated
05/05/05
2005-46
24
---
Not Rated
11/05/91
91-30
16
---
Not Rated
Data Analysis
Watershed -- headwater site is in the upper part of the watershed where it drains western Jones and southeastern Lenoir counties; no municipalities or NPDES
facilities in the rural watershed; very large tributary to the Neuse River. Habitats -- high quality habitats, score was 91 in 2005 (data were not collected in 1991);
narrow stream; coarse woody debris (submerged and snags); pools, and runs; mature Bald Cypress forest with open bottomlands. Water Quality -- specific
conductance was within the norm for a Neuse Coastal Plain stream, was 106 pS/cm in 2005 (data were not collected in 1991).
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- only 194 fish collected in 2015 contrasted to 627 collected in 2005; the increasingly uncommon and intolerant Ironcolor Shiner was absent in 2015, 74
specimens of this species had been collected in 2015. 1991-2015 -- 28 species known from the site including 7 species of sunfish, 4 species each of cyprinids,
catfish, and darters, and 1 species of sucker; dominant species are the intermediate Dusky Shiner (1991 and 2005) and the tolerant Redbreast Sunfish and
tolerant Eastern Mosquitofish (2015).
Most Abundant Redbreast Sunfish (38, Non -indigenous Imperiled
Species 20%) Species Bluegill (5) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
�_TUCKAHOE SWP
SR 1142
JF64
08/18/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
JONES
03020204
35.032226
-77.579442
40
No
Level IV Ecoregion
Drainage Area ( )
Stream Width (m)
Stream Depth (m)
Carolina Flatwoods
54.3
1 7
0.6
Upstream NPDES Dischargers (? 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
46.3
1.1
no data
28.9
no data
23.7
0.1
0.0
2001
32.1
4.0
0.5
26.9
16.0
20.5
0.1
0.0
2006
29.6
4.0
0.5
28.5
17.4
20.3
0.1
0.0
Water Quality Parameters
2000
2005
2015
Temperature (°C)
25.6
21.2
24.6
Dissolved Oxygen (mg/L)
4.4
4.0
3.7
Specific Conductance (pS/cm)
134
103
135
pH (s.u.)
7.0
6.3
6.5
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
8
Bottom Substrate (15)
1
Pool Variety (10)
10
Bank Erosion (10)
8
Bank Vegetation (10)
9
Light Penetration (10)
10
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
71
Water Clarity Clear/tannic, very dark, became very silty
Substrate: Deep and very soft muck, silt, and sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
08/18/15
2015-78
16
---
Not Rated
05/16/05
2005-50
23
---
Not Rated
06/12/00
2000-40
19
---
Not Rated
Data
Watershed -- drains southeastern Lenoir (including a portion of the Town of Pink Hill), western Jones, and a small area of northwestern Onslow counties; no
NPDES facilities in the watershed; tributary to the Trent River. Habitats -- moderate quality habitats, but the scores were greater in 2000 and 2005 (92 and 88,
respectively) than in 2015 (71); natural channel; coarse woody debris (deadfalls and submerged); deep pools; Bald Cypress forest along the riparian zones;
limestone outcrop on the left at the beginning of the reach and extending -- 30 yards upstream; beaverdam complex at end of the reach, old beaverdam at the
beginning of the reach , - 75 ft. upstream of the bridge. Water Quality -- specific conductance was within the norm for a Neuse Coastal Plain stream, has
ranged from 103 pS/cm in 2005 to 135 pS/cm in 2015; dissolved oxygen routinely low (- 4 mg/L) and at 44% saturation in 2015, but permissible under a
supplemental water quality classification of Sw.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- fewest species ever collected at this site; fewest fish (n = 97 vs. 424 in 2000 and 376 in 2005) ever collected at this site. 2000-2015 -- 27 species
known from the site including 7 species of sunfish, 4 species each of cyprinids and darters, 3 species of catfish, and 1 species of sucker; 3 Intolerant Species
(Ironcolor Shiner, Sawcheek Darter, and Chainback Darter) collected in 2005, the most Intolerant Species at any site in any year in the Neuse Coastal Plain;
species dominant species are the intermediate Dusky Shiner (2000), the tolerant Eastern Mosquitofish (2005 and 2015), and intermediate Bluegill (2015).
Most Abundant Eastern Mosquitofish (24, Non -indigenous Bluegill (21) and Redear Imperiled
Species 125%) Species Sunfish (5) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
TURKEY CR
SR 1109
JF54
04/30/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
NASH
03020203
35.803898
-78.179004
170
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Northern Outer Piedmont
38.3
8
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None I---
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
62.5
0.6
no data
24.4
no data
11.3
0.8
0.4
2001
48.2
7.3
1.1
31.7
2.8
8.5
0.8
0.1
2006
48.0
7.3
1.1
30.8
4.7
8.3
0.9
0.1
Water Quality Parameters
2005
2015
Temperature (°C)
---
15.3
14.4
Dissolved Oxygen (mg/L)
---
6.5
8.4
Specific Conductance (pS/cm)
---
65
63
pH (s.u.)
---
6.5
5.8
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
12
Bottom Substrate (15)
4
Pool Variety (10)
6
Bank Erosion (10)
3
Bank Vegetation (10)
9
Light Penetration (10)
9
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
68
Water Clarity I Slightly turbid/tannic
Substrate: Clay, sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
04/30/15
2015-23
16
---
Not Rated
05/02/05
2005-39
18
---
Not Rated
Data Analysis
Watershed -- drains southeastern Franklin and southwestern Nash counties; greatest percentage of its watershed in forest than any other Neuse Coastal Plain
watershed in 2015; an ecotonal stream between the Rolling Coastal Plain and the Northern Inner Piedmont; no NPDES facilities in the watershed; tributary to
Buckhorn Reservoir (Contentnea Creek). Habitats -- moderate quality, but were of higher quality in 2005 (score = 91); natural channel; slippery clay substrate
with severely eroding banks; infrequent instream habitats. Water Quality -- lowest specific conductance of any site in the Neuse Coastal Plain in 2015, was
also low at 65 pS/cm in 2005.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2015 -- fewest fish collected (n = 47) and lowest Percentage of Tolerant Fish (9%) from any Neuse Coastal Plain site in 2015; 1 of only 2 sites were the wide-
spread and intermediate American Eel was not collected. 2005 & 2015 -- 22 species are known from the site including 7 species of sunfish, 4 species each of
cyprinids and catfish, and 2 species of darters; suckers have never been collected at this site; 1 of only 7 Neuse Coastal Plain sites from which the White
Shiner was collected, it is an obligate colonial spawner on Bluehead Chub and Bull Chub nests, both species are unknown from this site which may explain the
shiner's rarity, indeed, only four specimens of White Shiner have been collected at this site (in 2005); dominant species are the intermediate Highfin Shiner
(2005 and 2015) and the intermediate Bluegill (2015).
Most Abundant Non -indigenous Imperiled
Species Highfin Shiner (9, 19%) Species Bluegill (7) Species None

FISH COMMUNITY SAMPLE
Waterbody
Location
Station ID
Date
Bioclassification
WATERY BR
NC 58
JF55
05/28/15
Not Rated
County
8 digit HUC
Latitude
Longitude
Elevation (ft)
Reference Site
GRE EN E
03020203
35.571295
-77.808865
65
No
Level IV Ecoregion
Drainage Area (miz)
Stream Width (m)
Stream Depth (m)
Southeastern Floodplains & Low Terraces
6.0
4
0.4
Upstream NPDES Dischargers (>_ 1 MGD or < 1 MGD and within 1 mile) NPDES Number Volume (MGD)
None
Landuse (%)
Forest
Developed
Impervious
Cultivation
Grass/Herb/Shrub
Wetland
Water
Barren
1992
10.8
5.5
no data
57.3
no data
26.3
0.0
0.0
2001
14.7
7.2
0.7
59.6
5.0
13.0
0.2
0.0
2006
13.8
7.2
0.7
60.0
6.4
12.3
0.2
0.0
Water Quality Parameters
2005
2015
Temperature (°C)
---
18.0
21.3
Dissolved Oxygen (mg/L)
---
6.8
6.8
Specific Conductance (pS/cm)
---
99
118
pH (s.u.)
---
5.5
5.7
Habitat Assessment Scores (max score)
2015
Channel Modification (15)
15
Instream Habitat (20)
13
Bottom Substrate (15)
10
Pool Variety (10)
10
Bank Erosion (10)
5
Bank Vegetation (10)
8
Light Penetration (10)
8
Left Riparian Score (5)
5
Right Riparian Score (5)
5
Total Habitat Score (100)
79
Water Clarity ISlightly turbid/tannic, became very turbid
Substrate: Gray sand
Sample Date
Sample ID
Species Total
NCIBI Score
NCIBI Rating
05/28/15
2015-39
18
---
Not Rated
04/21/05
2005-31
14
---
Not Rated
Data
Watershed -- smallest watershed which was assessed in the Neuse Coastal Plain in 2015; drains northwestern Greene and northeastern Wayne counties;
greatest percentage of its watershed under cultivation than any other Neuse Coastal Plain watershed in 2015; no municipalities or NPDES facilities in the
watershed; tributary to Contentnea Creek, site was - 0.9 mile upstream from its confluence with Contentnea Creek. Habitats -- moderately high quality
habitats, score was 77 in 2005; natural channel; moderate bank erosion; evidence of very high water; dense Oriental Privet growing within the riparian zones;
large coarse woody debris snags, deadfalls, and obstructions across the channel. Water Quality -- specific conductance was within the norm for a Neuse
Coastal Plain stream, was 99 pS/cm in 2005; pH less than 6.0 s.u. in 2005 and 2015, but permissible under a supplemental water quality classification of Sw.
NCIBI Scores and Ratings are not applied because metrics and scoring criteria development is still underway for wadeable Coastal Plain streams.
2005 & 2015 -- fewest species of any Neuse Coastal Plain site (along with Island Creek), only 19 species known from the site including 6 species of sunfish, 3
species of cyprinids, 2 species each of catfish and darters, and 1 species of sucker; dominant species is the intermediate Dusky Shiner; number of fish
collected during both cycles was fairly constant (n = 198 and 208).
Most Abundant o Non -indigenous Imperiled
Species Dusky Shiner(31,15/0) Species Bluegill(28) Species None