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Home My WebLink AboutNC0020290_Toxicity Results_20150512 • • P0 Box 7565 Elris Asheville,NC 28802 Phone: (828)350-9364 Fax: (828)350-9368 E-mail: Jim@etsnclab.corn 3 Environmental Testing Solutions,Inc. April 24,2015 Ms. Ruth 011is RECEIVEDIDENRIDWR Water Quality Lab&Operations, Inc. MAY 1 2 2 015 PO Box 1167 Banner Elk,NC 28604 Water Quality Permitting Section RE: ETS PROJECT NUMBER: 10518 Dear Ms. 011is: Enclosed are toxicity test results for samples from the Burnsville WWTP received by Environmental Testing Solutions,Inc. April 06 through April 10,2015. Parameter Test Procedure EPA Method Number Code Final Result Fathead Minnow(Pimephales promelas) THP6C Larval Survival and Growth, EPA-821-R-02-013 ChV>24% Test Method 1000.0 Note: ChV=Chronic Value. A numeric value representing the geometric mean of the highest concentration having no detectable impairment of survival or growth and the lowest concentration that does have a detectable impairment of survival or growth. If this test was performed as an NPDES requirement or by Administrative Letter, please enter the ChV value>24% on the Effluent Discharge Monitoring Form(MR-1)for the collection date April 06,2015 using the parameter code THP6C. Additionally,please sign and submit the original DWQ Aquatic Toxicity Form(AT-5)by May 31,2015. If you have any questions concerning these results,please feel free to contact me. Sincerely, T S tuner Laboratory Director This report should not be reproduced,except in its entirety,without the written consent of Environmental Testing Solutions,Inc. The results in this report relate only to the samples submitted for analysis. North Carolina Certificate Numbers: Biological Analyses: 37,Drinking Water: 37786,Wastewater: 600 South Carolina Certificate Number: Clean Water Act: 99053-001 •• . PO Box 7565 Fax: .(828 • ETS ,NC 28802 Phone:Ashev(828)ille350-9364 )350-9368 E-mail: Jim@etsnclab.com Environmental Testing Solutions,Inc. Date: April 24,2015 Effluent Aquatic Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Facility: Burnsville WWTP NPDES#: NC-0020290 Pipe#: 001 County: Yancey • Laboratory Performing Test: Environmental Testing So ions,ID Comments: Signature of Operator in Responsible Charge: ......m......40. ' Signature of Laboratory Supervisor: /p,� • Project: 10518 `J / Samples: 150406.02,150408.20,150410.01 Mail Original To: North Carolina Department of Envonment and Natural Resources DWQ/Environmental Sciences Branch 1621 Mail Service Center Start date: End date: Start time: End time: Raleigh,NC 27699-1621 04-07-15 04-14-15 1402 1327 Replicate number 1 2 3 4 Test Organisms Control Surviving number of larvae 10 10 10 10 Survival(%) 100.0 Outside supplier: Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.612 Aquatox,Inc. I Weight/original(mg/larvae) 0.611 0.595 0.573 0.668 Average wt/ 0.612 tng(mg) Hatch date: 04-06-15 %Effluent Surviving number of larvae 10 10 10 10 Hatch time: 1600-1700 1.5% Original number of larvae 10 10 10 10 Survival(%) 100.0 Weight/original(mg/larvae) 0.580 0.581 0.680 0.590 Average wt(mg) 0.608 %Effluent Surviving number of larvae 10 10 10 10 [3.0%J Original number of larvae 10 10 10 10 Survival(%) 100.0 Weight/original(mg/larvae) 0.624 0.577 0.662 0.590 Average wt(mg) 0.613 %Effluent Surviving number of larvae 10 10 10 10 RECEIVEDlDENRIDWR 6.0% Original number of larvae 10 10 10 10 Survival(%) 100.0 Weight/original(mg/larvae) 0.581 0.643 0.479 0.684 Average wt(mg) 0.597 MAY 1 2 2015 %Effluent Surviving number of larvae r. 10 10 10 9 12% Original number of larvae 10 10 10 10 Survival(%) 97.5 Water Flu lltY Permitting Sctect Weight/original(mg/larvae) 0.625 0.717 0.649 0.617 Average wt(mg) 0.652 iar %Effluent Surviving number of larvae 10 10 10 10 24% Original number of larvae 10 10 10 10 Survival(%) 100.0 Weight/original(mg/larvae) 0.562 0.670 0.605 _ 0.609 Average wt(mg) 0.612 Water Quality Data Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Initial I Final Initial I Final Initial I Final Initial j Final Initial I Final Initial I Final Initial I Final Control pH(SU): 7.50 7.53 7.52 7.36 7.57 7.42 7.65 7.43 7.84 7.37 7.77 7.59 7.75 7.57 DO(mg/L): 7.8 7.8 7.9 7.3 7.7 7.1 7.8 7.3 7.9 7.1 7.9 7.2 7.9 7.4 Temp.(°C): 24.6 24.5 24.7 24.6 24.7 24.6 24.8 24.5 24.7 24.5 24.7 24.7 24.7 24.5 High Concentration pH(SU): 7.41 7.45 7.54 7.37 7.48 7.29 7.52 7.48 7.70 7.26 7.55 7.48 7.69 7.52 DO(mg/L): 7.8 7.7 7.9 7.2 7.7 7.2 7.8 7.3 7.9 7.1 7.9 7.2 7.9 7.4 Temp.(°C): 24.8 24.3 24.8 24.6 24.8 24.8 24.9 24.6 24.9 24.5 24.9 24.4 24.7 24.6 Sample Information Sample 1 Sample 2 Sample 3 Control Analyses Survival Growth Collection start date: 04-05-15 04-07-15 04-09-15 Normal: Yes Yes Grab: . . w. Hom.Var. Yes Yes Composite duration: 24-h 24-h 24-h NOEC: 24% 24% Alkalinity(mg/L CaCO3): 21 22 30 34,34,34 LOEC: >24% >24% Hardness(mg/L CaCO3): 38 40 50 40,42,40 ChV: >24% >24% Conductivity(µrnhos/cm): 271 252 302 151-156 Method: Visual Insp. Dunnett's Total residual chlorine(mg/L): <0.10 <0.10 <0.10 Sample Temp.at Receipt(°C): 2.7 3.4 3.5 Survival Growth Effluent Critical Calculated Critical Calculated 1.5% 2.410 0.105 3.0% . 2.410 -0.039 6.0% . 2.410 0.394 12% 2.410 -1.056 Overall Analysis: 24% . . 2.410 0.007 Result: PASS LOEC: >24% NOEC: 24% ChV: >24% DWQ form AT-5(8/03) / ' • it Page 1 of 5 .: Environmental Testing Solutions.Inc. Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: Burnsville WWTP County: Yancey NPDES#: NC 0020290 Outfall#: 001 Project#: t0 St% Chronic Limit: 6.0% Dilution preparation information: Comments: Dilution prep(%) 1.5 3.0 6.0 12 24 Effluent volume(mL) 15 30 60 120 240 Diluent volume(mL) 985 970 940 880 760 Total volume(mL) 1000 1000 1000 1000 1000 Test organism information: Test information: Organism age: Z.\.G o '1:2-.(Pj 1-IteN.WIS OLD Randomizing template: faft.R. Date and times organisms oil-ot.•%S %.,(14)-1-1(='0 Incubator number and "E were born between: shelf location: Organism source: ATOX Batch Pp: oy•o0.•tS Artemia CHM number: CHM836 Drying information for weight determination: Transfer bowl information: pH= 1.t•\ S.U. Date/Time in oven: o.0e1S 1400 Temperature=1.4. 1°C Initial oven temperature: !op'C Average transfer volume: 0.1749 mL Date/Time out of oven: 04.1s-IS IA CO Final oven temperature: 4)0•C Total drying time: •L-Roact Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation,renewal, Sample SSW or termination number used batch used Time Analyst Time Analyst Time Analyst 0 04-07-15 ei.crv•S >+ 1$20 4I4 01— �( 1SCf`106.GL C4 GZ-t5 A 1 04-08-15 O 0o g \atO cy� 13 • U` (sotto,. 0 •LS i 2 04-09-15 0$00 1AD0 y{T' � • �.{ 4O6 +•U2 1. 13-1.. vx(` tSo4od. zo o'.l•C l.-tS S 3 04-10-15 OA 043 g ' -koo , 3-( tas Al Iso4oa-2 owl-oz►s 8 4 04-11-15 Dr00 J t`k00 ' bc.S gtSo'4 10.01 CM-07-tS 5 04-12-15 coo A( NW . g 13o•L ai tsoyto. i OM-CF- IS 6 04-13-15 coo R ty00 .t 01►S d ' 0410.01. 0y-o1-IS 04-14-15 1. _ , . .1 . s . PPj ` Control information: Acceptance criteria Summary of test endpoints: %Mortality: 01- s 20% 7-day LCso '24% Average weight per initial larvae: p•bt2 NOEC 24 7, Average weight per surviving larvae: 0.b\1.— >_0.25mg/larvae LOEC >241. ChV X24 7. IC25 )2%47. SOP AT20-Exhibit AT20.3,revision 11-01-14 Page 2 of 5 • e6 Environmental Testing Solutions.Inc. Species: Pimephales promelas Date: 04-07-1.5 Client: Burnsville WWTP Survival and Growth Data Day CONTROL 1.5% 3.0% A B C D F F G H I J K L 0 10 10 10 10 10 10 10 10 10 10 10 10 ' 10 10 '0 /0 10 / 6 10 /0 10 10 10 10 2 10 /0 10 10 to 10 10 to to to 10 ll� 3 10 10 10 10 10 10 /0 /0 /0 / 0 l0 l0 4 Ip 10 10 10 10 10 10 10 10 10 1p l0 5 10 10 10 / 0 10 /0 10 10 10 IQ 10 10 6 10 'u to 10 10 ' 0 10 10 10 �0 Ip ID 10 10 IQ 10 10 Ip l0 10 /0 /3 /0 10 A=Pan weight(mg) Tray color code:: Wor.S.1 Analyst: `b.� t1p.0(0 IS.19 Ie•2� 158 Ito.2q � y lc.IS- t583 Ho.z$ -��,r1Sy� Date: 0-6.3\-\s B=Pan+Larvae weight(mg) Analyst: 22.13 2Z.012.1.5Z 2295 21.5$ 22.10 22.4 21.05, 22.0122.06 22•(4I Date: 01-1-‘ --1S C=Larvae weight(mg)=B-A Hand calculated. to.\1 SAS 513 t.,.L Q s.ao s.$l 6.$b 5°10 (..i4 5.i-i b•'+Z. S.90 Analyst: Weight per initial number of larvae(mg) =C/Initial number of larvae An, \ N 0 ^ O .y., cb0 � Hand calculated. �y 1 L, • 4, moi' (� /Va �`o SA Analyst: r — 0 0 0 O' 0' 0 0 Co" 0. O O• Average weight perinitialPercent number of larvae(mg) reduction from control . 0."2 0.17. 0.b vb (%) Comment codes: c=clear,d=dead, fg=fungus,k=killed,m=missing, sk=sick,sm=unusually small, lg=unusually large,d&r=decanted and returned, w=wounded. Comments: SOP AT20—Exhibit AT20.3,revision I 1-01-14 Page 3 of 5 • -c" .a Environmental Testing Solutions,Inc. Species: Pimephales promelas Client: Burnsville WWTP Date: 04-07-15 Survival and Growth Data Day 6.0% 12% 24% MN 0 PQR S T U V W X 0 10 10 10 10 10 10 10 10 10 10 10 10 1 l0 16 10 10 / 0 /0 /0 /0 /0 /0 / 6 /0 2 10 t0 10 ID /0 ID 1p l0 1p /0 t0 /0 3 (D /b 1D 10 lb ID 10 IO lb /0 /0 /0 4 i0 10 to ID 10 10 10 1D 10 10 / 0 t0 5 10 10 10 ID 10 10 10 (0 /0 10 10 10 6 ID 10 IC) 10 lb (C) 10 q` /0 /0 10 10 7 ID r0 /0 10 10 /0 10 °I 10 10 10 10 A=Pan weight(tng) Tray color codei VUv, '.t Z2 140•)3 151&) I10TO (b.i2. is:11 11x.24 Analyst: tS,S� '6'1'511u.l�b lb Ito.117 1S•�3 Date: x'31.15 B=Pan+Larvae weight(mg) Analyst: 2I.3$ 2230 It$S Z3.O( 22.2$ 23.n Z2.2221.91 22.4-122.12 2.1.11.9 2233 Date: 04- c= wl-C=Larvae weight(mg)=B-A S.Q u 6.4'5 4.1a i,.13 1 1..1-S 1.11 V.%A t..►1 5.1.1. L.10 (..0S 6.04 Anacalculated. V Analyst: Weight per initial number of larvae(mg) =C/Initial number of larvae ^ ^^ ,17%' ^ ^O � Hand calculated. Analyst: 4`--�-�' p' v O O' 0 o V0 O o' O Average weight per initial Percent number of larvae(mg) reduction , _57, 0.� Z. -{x.101. 10lZ 4.07 from control (%) Comment codes: c=clear,d=dead, fg=fungus, k=killed,m=missing,sk=sick,sm=unusually small, Ig=unusually large,d&r=decanted and returned,w=wounded. Comments: SOP AT20—Exhibit AT20.3,revision 11-01-14 • il Ell.S Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013,Method 1000.0 0 Quality Control Verification of Data Entry,Calculations,and Statistical Analyses 411 Environmental Testing Solutions,Inc. Client: Burnsville WWTP Test dates: April 07-14,2015 Project number. 10518 Concentration(%) Replicate 1.ItW.amber of Final amber of larvae A Pan weight a-Pan+Larvae lanae weight(mg) Weight/S.rvhi.g Mean weight/ Caeltkleat/*variation Weight/Initial umber Mean servivel Mean wdghtl Coefficient et Percent reduction from larvae (mg) anighl(mg) aA-B number of lamas(mg) Swelling somber of se.w..W rwaew4 of larvae(nig) (%) Initial anmberof variation(%) control(%) larvae(toe -►r nr.wt(%) larvae(mg) A 10 10 16.62 22.73 6.11 0.611 0.611 Control B 10 10 16.06 22.01 5.95 0.595 0612 6.6 0.595 100.0 0.612 6.6 Not applicable C 10 10 15.79 21.52 5.73 0.573 0.573 D 10 10 16.27 22.95 6.68 0.668 0.668 E 10 10 15.78 21.58 5.80 0.580 0.580 1.5% F 10 10 16.29 22.10 5.81 0.581 0.608 8.0 O.S81 100.0 0.608 8.0 0.7 G 10 10 15.68 22.48 6.80 0680 0.680 H 10 10 15.15 21.05 5.90 0.590 0.590 I / 10 10 15.83 22.07 6.24 0.624 0.624 J 3.0% 10 10 16.28 22.05 5.77 0.577 0613 62 0.577 100.0 0.613 6.2 -0.2 K 10 10 15.79 22.41 6.62 0.662 0662 L 10 10 15.47 21.37 5.90 0.590 0.590 M 10 10 15.57 21.38 5.81 0.581 0.581 6.0% N 10 10 16.37 22.80 6.43 0.643 0.597 14.9 0.643 100.0 0.597 14.9 2.5 0 10 10 14.06 18.85 4.79 0.479 0.479 P 10 10 16.22 23.06 6.84 0684 0.684 Q 10 10 16.03 22.28 6.25 0.625 0.625 12% R 10 10 16.10 23.27 7.17 0.717 0.717 S 10 10 15.73 22.22 649 0649 0669 6.0 0649 97.5 0.652 7.0 -6.6 T 10 9 15.80 21.97 6.17 0.686 0.617 • U 10 10 16.80 22.42 5.62 0.562 0.562 24% V 10 10 16.12 22.82 6.70 0.670 0 612 7.3 0.670 100.0 0.612 7.3 0.0 W 10 10 15.11 21.16 6.05 0.605 0.605 X 10 10 16.24 22.33 6.09 0.609 0609 Outfall 001: MSD= Minimum Significant Difference Dunnett'.MSD value: 0.0918 PMSD- Percent Minimum Significant Difference PMSD: 15.0 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA(10th percentile)-12X. Upper PMSD bound determined by USEPA(90th percentile)...30%. Lower and upper PMSD bounds were determined front the 10th and 90th percentile,respectively,of PMSD data from EPA's WET Interlaboratory Variability Study(USEPA,2001a;USEPA, 2001b). USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-B-01-004 and EPA-821-B-01-005.US Environmental Protection Agency,Cincinnati,OH. File:bumsville_040715pp.xls Entered by J.Sumner Reviewed by: • • Environmental Testing Solutions,Inc. Larval Fish Growth and Survival Test-7 Day Growth Start Date: 4/7/2015 Test ID: PpFRCR Sample ID: Burnsville WWTP End Date: 4/14/2015 Lab ID: ETS-Envir.Testing Sal. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-% 1 2 3 4 D-Control 0.6110 0.5950 0.5730 0.6680 1.5 0.5800 0.5810 0.6800 0.5900 3 0.6240 0.5770 0.6620 0.5900 6 0.5810 0.6430 0.4790 0.6840 12 0.6250 0.7170 0.6490 0.6170 24 0.5620 0.6700 0.6050 0.6090 Transform:Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6118 1.0000 0.6118 0.5730 0.6680 6.638 4 0.6163 1.0000 1.5 0.6078 0.9935 0.6078 0.5800 0.6800 7.960 4 0.105 2.410 0.0918 0.6163 1.0000 3 0.6133 1.0025 0.6133 0.5770 0.6620 6.207 4 -0.039 2.410 0.0918 0.6163 1.0000 6 0.5968 0.9755 0.5968 0.4790 0.6840 14.946 4 0.394 2.410 0.0918 0.6163 1.0000 12 0.6520 1.0658 0.6520 0.6170 0.7170 6.966 4 -1.056 2.410 0.0918 0.6163 1.0000 24 0.6115 0.9996 0.6115 0.5620 0.6700 7.265 4 0.007 2.410 0.0918 0.6115 0.9922 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution(p>0.01) 0.93105 0.884 -0.0349 0.28944 Bartlett's Test indicates equal variances(p=0.67) 3.18555 15.0863 Hypothesis Test(1-tail,0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 24 >24 4.16667 0.09185 0.15014 0.00142 0.0029 0.77945 5,18 Treatments vs D-Control Linear Interpolation(200 Resamples) Point % SD 95%CL(Exp) Skew IC05 >24 IC10 >24 IC15 >24 1.0 IC20 >24 0.9 IC25 >24 IC40 >24 0.8 IC50 >24 0.7 0.6 W 0.5 a 0.4 ffi 0.3 0.2 0.1 0.0•••-• --♦ -0.1 -0.2 0 10 20 30 Dose% Dose-Response Plot 0.8 0.7 0.6 f -1-tail,0.05 level 10.5 _ of significance 0 O 0.4 0.3 0.2 0.1 0 v M co n v C N C d File: bumsville 040715pp.xls Entered by: 1 inner Reviewed by • Page 4 of 5 ET.I Environmental Testing Solutions,Inc. Species: Pimephales pramelas Date: 04-07-15 Client: Burnsville WWTP Dail Chemist : Day (Anal st identified for each da , .erformed .H,D.O.and conductivi measurements onl . 0 1 2 Anal st MIAMI IIMMINIMW Concentration Parameter CONTROL 4 ��' 1.S.1 1•S2 �r�r'�E l•tn- DO(m: MEM EMI. Conductivity 521G3IME mhos/cm) J . • *Alkalinity 3-13� (m_CaCO3/L) *Hardness `� (ml CaCO3/L) *Tem terature(°C) EOM MUM 1A--I `k b IB ' 2`{. Ek 1• 0 .too i •310 MEM EIMM 1.5% DO m: ) MI _MIME NM.MM. Conductivity mhos/cm) 155 (5�t + 1 (5% MEM> 1.:4-6 MOM MUM 141 MONIMI rEfin MUM! `1.5o 3.0% DO(m_ ) IMMIMIEMINIMEEM 1.2 MIEN MMEil Conductivity mhos/cm) 555 15`a (S� *Tem.erature(°C) MEM 'I-A. to MEM EMU MEM IMMINE REMEl=1111 MT= -1• q 1•SS 11111gM 6..0% DO m_ IIEME MIME MO ' -t_7- Conductivity 15 l too 1.L mhos/cm 01:1 -1.3\ 12% DO m: MIMI ME'EWq_�� "1 MEM � Civi mhos/cmri Ito 1(0O 1(61 1110110111 1W3H11=JERI UMW 1y• NEE=IRISIMIII EM1111 7 24% DO m: �:�MiS7 1M MINI kW= Conductivity t -1S k o ( mhos/cm) l KOM=STRI MMIL7 —1.9•a MUM WWII MEM" 100% DO m: ) =gm Canductivity 2 1 I 262 ( mhos/cm *Alkalinity Z` ZZ m:CaCO3/L) *Hardness 3� , /0 (m:CaCO3/L) `T *TR chlorine(mg/L) 0,10 '40 .1O 25.0 Initial Final Initial Final Initial Final *Temperatures performed at the time of test initiation,renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity,hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: 0%l( . SOP AT20—Exhibit AT20.3,revision 11-01-14 a Page 5 of 5 9c y .t Environmental Testing Solutions,Inc. Species: Pimephales promelas Date: 04-07-15 Client: Burnsville WWTP Day (Anal st identified for each da , Ierformed.H,D.O.and conductivi measurements onl . 3 4 5 6 Anal st up I 41 MINMEIMg=EMI11111raM 1Lc.J Concen- Parameter tration CONTROL IMMIIMIll —1.(.t)s MEM M}a.MIMI MEM MM.REIM i"RtFIR_ DO m: n ��M� _ Conductivity mhos/cm l 52 =' ISI•', IMI t S(40 *Alkalinity a m:CaCO3/L �.`�`]fiMil 3 ` ME • *Hardnessim ,1 0 MI m:CaCO3/L) �1 *Tem,erature(°C) 1-•t Zy -S -1-1/4A-1 Z'k,S MEM '1.A.1 &Mai -t,`1•S ITEMBI EMI'MEM MIMI MIMI REM MIME MOM tai 1 1.5% DO(m: ME=MEW .9 Ik1=1 - NEIMI MI 'MIMS Conductivity I6 1 bD IS-1 1 I- mhos/cm REMEEraMMI -1.`-1-'\ WWII -N-41 t' .1 IS.8 INMENNEUEMMENI _ ZE o �� &M�1 3.0% DO(m: Conductivity mhos/cm) I55 162 1 I(i, 1.51 WEN IE WEI MEWS KIM IFIKM EWEs', 'Tal - 6..0% DO m:L MIMI Mail MUM WM MEM '1.Z Mg'EtallNil Conductivity C� f, En mhos/em I ` IMEMEME11 MOM MEM MM.MEM 1k-1.t —LA•S ROM MIMI 12% DO(m: Mk.3 INGIMIIIMMIM16111 "1.Z WEIIIIi' Conductivity mhos/cm) `u<-1 tlii I-41 ti C0 *Tem,erature(°C) UMW 1a•b MEM 1..1-1.10 1111VM MEM -1,4-1 MM ITErar 1.52 NEM artalaiiiinkViEigri SEM MEM 1.52 24% DO(m: ) MEM Illan' 'MEM '��USMOM Conductivity t-1 S (�2 in ( mhos/cm) I rrigi rIMIE' 100% DO(m• Conductivity 302 mhos/cm) *Alkalinity ^ (m:CaCO3/L () *Hardness S O (m:CaCO3/L *TR chlorine(mg/L) 4O.tO *Tem i erature(°C) IS•0 Initial I Final Initial Final Initial I Final Initial I Final *Temperatures performed at the time of test initiation,renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity,hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: . SOP AT20—Exhibit AT20.3,revision 11-01-14 • • 351 Depot Street . ETs Asheville,NC 28801 Phone: (828)350-9364 ,® Fax: (828)350-9368 �+IV Environmental Testing Solutions,Inc. Whole Effluent Toxicity Chain-of-Custody Form Facility: Water Quality NPDES#: NC0020290 Pipe#: 001 County: Yancey Burnsville WWTP Purchase order: Species: Pimephales promelas Effluent dilution: 1.5,3.0,6.0, 18,36% Test type: Chronic Definitive Parameter code: THP6C Sample information: (to be completed by sample collector) Composite sample: //�� Sample location: ,�F-f Start date: ;" /5Time: 'O't1 Volume collected for testing: 1-Er End date: 14s 6 ^' Time: eat Number of containers filled for testing: Number of samples per hour: 11. Method of transport to laboratory: C`/el r ize r' Chilled during collection? v. a p . Comments: J If chilled,specify temperature: ,m Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: Pnnt Sigaatlme Due and time / Relinquished by: Received by: Yj p4;' PXY 5 ee,l1i,J i 0_, INN Print acme Due and time Print / Signature pate and woe Relinquished by: Received by: [. ........... . L'' H Print Signature Dale and time Print Signature Date and time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: / Received at ETS by: 1Ki5/� /✓A,� G /l , It)&- V.-- 104.041,1--,� Print igunve pue and time Print Signaturel e and me Custody seals intact?: _ 1111 '7 Sample temperature upon receipt at ETS(°C): Yes No Not used LA`t' Samples received in good condition?: _ _ Yes No Total residual chlorine upon receipt at ETS: _ 1±1 (DPD Presence/Absense Indicator,MDL=0.10 mg/L) Pn:amt ^bait Tracking number: ltI t'C `S SOL/b0.07— - Project number:Ime Sample number.. i.J Comments: • • 351 Depot Street • E IT S Asheville,NC 28801 Phone: (828)350-9364 Fax: (828)350-9368 3 Environmental Testing Solutions,Inc. Whole Effluent Toxicity Chain-of-Custody Form Facility: Water Quality NPDES#: NC0020290 Pipe#: 001 County: Yancey Burnsville WWTP Purchase order: Species: Pimephales promelas Effluent dilution: 1.5,3.0,6.0, 18,36% Test type: Chronic Definitive Parameter code: THP6C Sample information: (to be completed by sample collector) Composite sample: Sample location: 1;',F/L Start date: 1,--'7--J. _ Time: I O 0 Volume collected for testing: \-V End date: 1)••/7='4....5 Time: clef) Number of containers filled for testing: / Number of samples per hour: if f% Method of transport to laboratory: Ge a V/ e r Chilled during collection? y t�'5 Comments: If chilled,specify temperature: I Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: 'reycid q yew Print at...../.. ---unDate and time Relinquished by: Received by: ------"tt Prim /Signature Date and tune Print �,/ Signatwe Date and time Relinquished by: Received by: 6. Print Signature Date and time Print Signawe Date and time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: Received at ETS by: 1153 Print gnanne 5 - 5j Date and time Print Signature Dae a�tine Custody seals intact?: _ _ M Sample temperature upon receipt at ETS(°C): Yes No Not used Say- / Samples received in good condition?: J - Yes No Total residual chlorine upon receipt at ETS: _ N N (DPD Presence/Absense Indicator,MDL=0.10 mg/L) Present Absent Tracking number: Project number: 10516 Sample number: L S oe.24D Comments: . . • • 351 Depot Street Asheville,NC 28801 „,„• E11- 5 Phone: (828)350-9364 Fax: (828)350-9368 Environmental Testing Solutions,Inc. Whole Effluent Toxicity Chain-of-Custody Form Facility: Water Quality NPDES#: NC0020290 Pipe#: 001 County: Yancey Burnsville WWTP Purchase order: Species: Fimephales promelas Effluent dilution: 1.5,3.0,6.0, 18,36% Test type: Chronic Definitive Parameter code: THP6C Sample information: (to be completed by sample collector) Composite sample: Sample location: ./'----- Start /�/' Start date: 7-- I-5 Time: 9, ,Q Volume collected for testing: 1„• ( End date: 6-fr.-0 jQ--J 5 Time: yroyj Number of containers filled for testing: f Number of samples per hour: y� Method of transport to laboratory: G. . r) ie t^ Chilled during collection? Y.e$ Comments: If chilled,specify temperature: .,...ciG Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: /Ciss.1 Jac q le' Prim Signature Date and tune Relinquished by: Received by: ils....-.10*-1,5 (s.7. ..___.(1 Li'-e§-,./55/ ;5-ceid (-AS\ j )( .-/Vitiroi Print Si adue Date and tune Print tgnaure Date and time Relinquished by: Received by: . Ilk- ..._._.___d Prim Sumature Date and time Print Signaure Date and tum Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: Received at ETS by: ,/ y-lo-fc 0,1,to,i . Wt,(F.l> 64-7-7-7\ l 03 i f Ihc1 - UL /03 Print Signatwo Date and tmrc Print Sigiutwe Dae and time Custody seals intact?: — IIIIII -, Sample temperature upon receipt at ETS(°C): /n1 yy Yes No Not used �/�v -` Samples received in good condition?: ' — Yes No Total residual chlorine upon receipt at ETS: — (DPD PresenceJAbsense Indicator,MDL=0.10 mg/L) Present Absent Tracking number: Pt('C �]]�� '�q 1i10.OI Project number: io` le Sample number: Comments: • Pimephales promelas Chronic Reference Toxicant Control Chart ® Organism Source: Aquatox, Inc. 6 Environmental Testing Solutions,Inc. 1.0 I 1 I 1 I 1 I III I I I I I 1 1 I I I USEPA Control Limits (± 2 Standard Deviations) 0.9 – – 0.7 – ............................................................. ................ ................................... •• – 0.6 – – 0.5 – – 11111111111111111111 1.4 II I 1 1 I 1 I I I I I I I I I I I I I USEPA Warning and Control Limits (75th and 90th Percentile CVs) H no V 0.6 – – co I 1 1 I 1 1 A 1 _I L 1 I I 1 1 I 1 1 I 1 1.4 11 I I I I 1 ill I I 1 I I ' Till Laboratory Warning and Control Limits (10th and 25th Percentile CVs) 1.2 – 1.0 – – 0.8 – – — 0.6 – – 0.4 – – I I 1 1 1 1 I 1 1 1 I I_ I I 1 1 I 1 1 �1.1C,1%.0 A0.0s rf 0�� 1�1Q1� 101 151 osj3 ,L1��1�111 11 110.01,L,L1�A3.1az S OA i5 Test date ---�– 7-day IC25= 25%inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. — — Central Tendency(mean IC25) — — - Warning Limits (mean IC25±SA.10 or SA.75) Graphs generated from associated excel spreadsheet. Control Limits(mean IC25±SA.25,SA.90, or 2 Standard Deviations) Excel c s bvsheetenter.�py:J.Sumner