NC0020290_ETS Project (10588)_20150222

RECEIVEDIDENRIDWR
May 22, 2015
Ms. Ruth 011is MAY 2 6 2015
Water Quality Lab & Operations, Inc. Water Quality
PO Box 1167 Permitting Section
Banner Elk, NC 28604
RE: ETS PROJECT NUMBER: 10588
Dear Ms. 011is:
Enclosed are toxicity test results for samples from the Burnsville WWTP received by Environmental
Testing Solutions, Inc. May 04 through May 08, 2015.
Parameter Test Procedure EPA Method Number
Code Final Result
Fathead Minnow (Pimephales promelas)
THP6C Larval Survival and Growth, EPA -821-R-02-013 ChV > 24%
Test Method 1000.0
Note: ChV =Chronic Value. A numeric value representing the geometric mean of the highest concentration having no
detectable impairment of survival or growth and the lowest concentration that does have a detectable impairment of survival or
growth.
If this test was performed as an NPDES requirement or by Administrative Letter, please enter the ChV
value >24% on the Effluent Discharge Monitoring Form (MR -1) for the collection date
May 04, 2015 using the parameter code THP6C.
Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT -5) by
June 30, 2015.
If you have any questions concerning these results, please feel free to contact me.
Sincerely,
Jim umner
Laboratory Director
This report should not be reproduced, except in its entirety, without the written consent of Environmental Testing Solutions, Inc.
The results in this report relate only to the samples submitted for analysis.
North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
*
!
PO Box 7565
Asheville, NC 28802
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Environmental Testing Solutions, Inc.
RECEIVEDIDENRIDWR
May 22, 2015
Ms. Ruth 011is MAY 2 6 2015
Water Quality Lab & Operations, Inc. Water Quality
PO Box 1167 Permitting Section
Banner Elk, NC 28604
RE: ETS PROJECT NUMBER: 10588
Dear Ms. 011is:
Enclosed are toxicity test results for samples from the Burnsville WWTP received by Environmental
Testing Solutions, Inc. May 04 through May 08, 2015.
Parameter Test Procedure EPA Method Number
Code Final Result
Fathead Minnow (Pimephales promelas)
THP6C Larval Survival and Growth, EPA -821-R-02-013 ChV > 24%
Test Method 1000.0
Note: ChV =Chronic Value. A numeric value representing the geometric mean of the highest concentration having no
detectable impairment of survival or growth and the lowest concentration that does have a detectable impairment of survival or
growth.
If this test was performed as an NPDES requirement or by Administrative Letter, please enter the ChV
value >24% on the Effluent Discharge Monitoring Form (MR -1) for the collection date
May 04, 2015 using the parameter code THP6C.
Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT -5) by
June 30, 2015.
If you have any questions concerning these results, please feel free to contact me.
Sincerely,
Jim umner
Laboratory Director
This report should not be reproduced, except in its entirety, without the written consent of Environmental Testing Solutions, Inc.
The results in this report relate only to the samples submitted for analysis.
North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001

i
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e
ETS
'S Environmental Testing Solutions Inc
PO Box 7565
Asheville, NC 28802
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Date: May 22, 2015
Effluent Aquatic Toxicity Report Form - Chronic Fathead Minnow Multi -Concentration Test
Facility: Burnsville WWTP NPDES #: NC- 0020290 Pipe #: 001 County: Yancey
Laboratory Performing Test: Environmental Testing Soluti s Inc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: Pro'ea: 10588
Samples: 150504.01. 150506.34, 150508.24
Mail Original To: North Carolina Department of Environmen{and Natural Resource.-
DWQ/ Environmental Sciences Branch
1621 Mai] Service Center
Raleigh, NC 27699-1621
Start date: I End date: Start time: End time:
05-05-15 1 05-12-15 1135 1039
Survival (%)
100.0
Average wt(mg)
Replicate number
Average wt /
0.614
surviving (mg)
1
2
3
4
Control[Surviving
number of larvae
10
1 10
10 1
10
Organisms
loriginal number of larvae
10
1 10
10 1
0
0.573
wei ttori nal (mg/larvae)
0.526
0.600
0.652
0.679
%Effluent
ISurviving number of larvae
10
10
10
10
1.5 %
loriginal number of larvae
10
1 10
10
10
10
Wei tori 'nal (m arvae)
1 0.614
1 0.584 1
0.675 1
0.651
Start date: I End date: Start time: End time:
05-05-15 1 05-12-15 1135 1039
Survival (%)
100.0
Average wt(mg)
0.614
Average wt /
0.614
surviving (mg)
F -24'/D.
Survival (%) 100.0
Average wt (mg) 1 0.631
% Effluent
I Surviving number of larvae
10
10
1 10
9
24%
F -24'/D.
3.0 EK
Original number of larvae
10
10
1 10
10
Survival (%)
978
Wei tori ' ( ae)
Weight/original (m ae)
0.569
0.578
0.568
0.577
Average wt (mg)
0.573
%Effluent
ISurviving number oflarvae
10
10
10
10
LtitialI Final
6.0 %
Original number of larvae
10
10
10
10
Survival
weightlori (mg/larvae)
0.594
0.637
0.576
0.576
Ave-agewt(mg)
0.596
%Effluent
ISurviving numberoflarvae
10 1
10
1 10
1 10
-1r/Dl
Original number of larvae
1 10 1
10
1 1010
1
Survival
wri t/ori inal (m arvae)
1 0.609 1
0.649
1 0.606
0.612 1
Average wt (mg)
0.619
Test Organisms
Outside su pliers
A uatox, Inc.
Hatch date: OS -04-15
Hatch time: 1 1600-1700
%Effluent
I Surviving number o£larvae
10 1 10
10 1
10
24%
24%
F -24'/D.
Original number of larvae
10 1 10
1 10
10
Survival
12%
2.410
-0.153
Wei tori ' ( ae)
0.602 1 0.556
1 0.458
0.583
Average wt (mg)
1 0.550
Water Quality Data
Day 0
Day 1 I
Day 2
1 Day 3 Day
4 1 Day 5
D
LtitialI Final
initial I Final I Initial I Final
I Initial I Final Initial I
Final I Initial I Final
initial I Final
Control
pH (SU):
DO (mg/L):
Temp. (°C):
High Concen
pH (SU):
DO (mg/L):
Temp. (°C):
7.40 7.15 7.32 1 6.92 7.29 6.94 1 7.27 1 7.03 1 7.25 1 6.94 1 7.27 7.00 7.22 6.96
7.9 7.7 8.0 1 7.6 7.9 7.2 1 8.0 7.3 1 8.0 1 7.2 1 7.9 7.8 8.0 7.8
24.7 24.5 24.8 1 24.6 24.7 24.4 1 24.7 24.5 1 24.8 1 24.6 1 24.7 24.8 24.8 24.5
ration
E
PEjP2E50
6.98 7.22 6.80 7.25 7.08 715 6.90 7.12 6.93 7.11 6.91
7.5 8.0 6.5 8.0 7.4 8.0 7.2 7.9 7.9 8.1 7.7
24.8.7. 24.5 24.7 1 24.5 1 24.9 24.5 1 24.7 1 24.5 L 24.8 1 24.7 1 25.0 1 24.3
Sample Information
Collection start date:
Grab:
Composite duration:
Alkalinity (mg/L CaCO3):
Hardness (mg/L CaCO3):
Conductivity (pmhos/cm):
Total residual chlorine (mg/L):
Sample Temp. at Receipt (°C):
Analyses
Normal:
Hom. Var.
NOEL:
LOEC:
ChV:
Method:
Overall Analysis:
Result: PASS LOEC: >24% NOEC: 24% ChV: >24%
Survival Growth
Yes
Yes
Yes
Yes
24%
24%
>24%
>24%
>24%
>24%
Visualln
Dunnett's
Survival Growth
%Effluent Critical Calculated
Critical
Calculated
1.5%
2.410
-0.541
3.0%
2.410
1.332
6.0%
2.410
0.597
12%
2.410
-0.153
24%
2.410
2.083
DWQform A T-5 (8/03)

:FT,S
Environmental Testing Solutions, lnc.
Chronic Whole Effluent Toxicity Test (EPA -821-R-02-013 Method 1000.0)
Species: Pimephales promelas
Client: Burnsville WWTP
NPDES #: NC 0020290
Project #: _ l 0z t$
County: Yancey
Outfall #: 001
Chronic Limit: 6.0%
Dilution preparation information: Comments:
Dilution prep (%) 1 5 3.0 6.0 12 24
Effluent volume (mL) 15 30 60 120 240
Diluent volume (mL) 985 970 940 880 760
Total volume (mL) 1000 1000 1000 1000 1000
Page 1 of 5
Test organism information:
Test information:
Organism age:
l $ . S& T6 1o,, SS fio 0 ub
Randomizing template:
e�
Date and times organisms
were born between:
Time Anal st
Incubator number and
shelf location:
�-
Organism source:
ATOX Batch Pp: oS
Artemia CHM number:
CHM836
Drying information for weight
determination:
Transfer bowl information:
pH= 1-S1 S.0
Temperature = %l•iPC
Date / Time in oven: 0S•+z•%S
Initial oven temperature: L V L
Average transfer volume:
0.1749 mL
Date / Time out of oven: OS i3 +S
Final oven temperature:
Total drying time: -L`� FbJttS
Daily feeding and renewal information:
+3oa
1-bod
Day
Date
Morning feeding
Afternoon feeding
Test initiation, renewal,
or termination
Sample
number used
SSW
batch used
Time Anal st
Time
Analyst
Time
Analyst
0
05--15
7 24 7.
t•{S
11
IS oSpA-ok
o+21-6 A
�• is
1
05-0606-15
OCoa
+`-100
035
-ststA.ok
ok-1i-tsA
2
05-07-15
p OU
IS 0S C)f - 3
43+21 -Is
3
05-08-15
6Cm
%SOsm.- `V
0'i•2.1-ISg
4
05-09-15
US
t 03S
ISO50 •2
0-1--n-isg
5
05-10-15
U�'SO
�3'�O
t 014
aw
6
7
05-11-15
OS -12-15
of ,ll
l0'j�
(SOS 04- -L`I I os -n- fS
Control information:
Acceptance criteria Summaq of
test endpoints:
% Mortality: 01,
s 20% 7 -day LC50
> Z`�'�•
Average weight per initial larvae: 6.6%4
ISM C
Z�
Average weight per surviving larvae: O-bN
>_ 0.25,0arvae LOEC
Z ?,
ChV
IC25
7 24 7.
SOP AT20 — Exhibit AT20.3, revision 11-01-14
•K

:ETS
Environmental T"tlnq 5nludms. lnc.
Species: Pimeph ales promelas
Client: Burnsville WWTP
Survival and Grnwth Data
Page 2 of 5
Date: 05-05-15
Day
CONTROL
1.5%
3.0%
A
B
C
D
E
I F
G
H
I
J
K
L
0
10
10
10
10
10
10
10
10
10
10
10
10
1p
10
r4
to
to
to
rp
t0
/0
10
/0
2
!o
ro
tp
�C)
r0
rp
t0
(0
10
to
10
io
3
lU
10
(p
1p
1p
rp
1p
rp
10
to
►p
1p
4
rp
rp
rp
t0
1p
1p
�p
rp
tp
5
-----
10
1D
r0
ro
ro
to
to
6
��
l0
t0
rD
t0
lD
r p
1p
tD
�p
rp
! p
7
/p
to
to
10
to
rp
r p
10
r0
10
16
A = Pan weight (mg)
codV� �A
Analyst:
Anal st:
15,3
15.11
lb•3D
15.93.
l7
�y•92
'I '1
�tD.29
13017
116N
I6•16
15-T1
1y91
11 -bo
Date: Oti-?o �ls
B = Pan + Lary a weight (mg)
Analyst:
ZD��
L1.1�
22.42
?2.62
21.(aly
ZZ.13
zo.�Z
ZZ
l� $5
Z1.1oS
20.05'
22.01
Date:
C = Larvae weight (mg) = B - A
Hand calculated.
5,Zb
b.00
1•_52
Io,1a1
b•��
&�
le,1S
b.S�
S.bq
5."18
S.b$
5.1`1
Analyst: tom---'
Weight per initial number of larvae (mg)
= C / Initial number of larvae
Hand calculated.��v0
p0
Analyst
o
o
o•
ooa
o•
o
p
u
p'
S
p
o
p
Average weight per initial
Percent
number of larvae (mg)
reduction
C) • b I'A
Q • bZ�}
- Z -1 7•
1
• 513
1,.1
from control
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small,
Ig = unusually large, d&r = decanted and returned, w = wounded.
SOP AT20 — Exhibit AT20.3, revision I 1-01-14

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•
EnvM°nnretalT°sWg Sdutbns.Inc
Species: Pimephales promelas
Client: Burnsville WWTP
Survival and Growth Data
Page 3 of 5
Date: 05-05-15
Day
6.0%
12%
24%
M
N
1 O
P
Q
R
S
T
U
V
1 W
X
0
10
10
10
10
10
10
10
10
10
10
10
10
1
to
(o
1p
10
10
t0
t0
to
t0
to
to
/0
2
10
10
10
l0
1D
10
10
tp
1p
/0
10
10
3
t0
10
10
10
10
10
10
1p
10
1Q
10
1Q
4
Ip
10
10
10
10
10
/0
/0
!p
10
10
10
5
10
1p
t0
10
l0
10
l0
10
10
(0
(0
t0
6
�O
10
1p
►0
ro
10
1p
tp
1p
co
t0
10
7
10
10
10
10
/0
10
/0
1p
A = Pan weight (mg)
Tray color codec N—I U
Analyst: 1tt�J
15•x2
IS.y
13.4�j
134
g(r51t@.oq
I(i.lf{
15.3
IS•59
600
t619
1(0."6
Date:
B = Pan + Larvae weight (mg)
Analyst: J{ri
Date: D6•�j•�S
2D.910
183
A.20
Iq.22
0.34
72.S3
all
21.43
1.1.1
21. DO
21.11?
22.1$
C =Larvae weight (mg) = B — A
Hand calculated.
5 qti
V.31
5_110
S.lb
(.,pq
(..4q
6 0
4.1'L
(,.
S. S6
q.Sd
5.13
Analyst:
Weight per initial number of larvae (mg)
= C / Initial number of larvae
Hand calculated.yah'
��
y�`D
y^�
J%yah
�o
.o
adv
yaSb
aS�
%4.5Analyst:
b•
p'
O'
O'
O'
�'
O•
O'
O'
O
O'
or
Average weight per initial
Percent
number of larvae (mg)
reduction
0, SNO
aa. 67.
O tg
1.57
from control0•�b
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small,
Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
SOP AT20 — Exhibit AT20.3, revision 11-01-14

� Pimephales promelas Chronic Whole Effluent Tonicity Test
.N111111111111111 EPA -821-R-02-013, Method 1000.0
Quality Control
Verification of Data Entry, Calculations, and Statistical Analyses
Environmental Testing Solutions, Inc. Client: Burnsville WWTP
Test dates: May 05-12,2015
Project number: 10588
Coseeotnaw (%)
Anak.M
Wlkl aonberd 11todnmberd1arvw
laevw
A-Pwwtlaht
(mv
a-Pu+Lwow
welSht (ow
larvwwetaht(mV
-A-■
Welaht/Se"Wi,
aubrdkrvw(mV
Meso welaht/
Sorrlrlasaaatbrd
Isnw(s
Caealekwdvsrladw
�-+Mewsv�►r♦
amew erl-.wl(%)
Wel4N/Ia1lWnmber
dlwrae (�)
Meo wrvhal
(%)
Mao weld
to Is". (no
Cael6de.1d
varktb (%)
Ps trMwtlw nom
twhal (%)
Control
A
10
10
15.31
20.57
5.26
0.526
0.614
11.0
0.526
100.0
0.614
11.0
Not applicable
B 10 10 15.17 21.17 6.00 0.600
p,60p
C 10 10 16.30 22.82 6.52 0.652
0.652
0.679
D 10 10 15.83 22.62 6.79 0.679
15%
E
10
10
14.92
21.06
6.14
0.614
0.631
6.4
0.614
100.0
0.631
6.4
2.7
F 10 10 16.29 22.13 5.84 0.584
0.584
C 10 10 13.97 20.72 6.75 0.675
0.675
0.651
H 10 10 15.91 22.42 6.51 0.651
3.0%
1
10
10
15.16
20.85
5.69
0.569
0.589
5.9
0.569
97.8
0.573
0.9
6.7
3 10 10 15.87 21.65 5.78 0.579
0.578
K 10 10 14.97 20.65 5.68 0.568
0.568
0.577
L 10 9 16.30 22.07 5.77 0.641
6.0%
M
10
10
15.02
20.96
5.94
0.594
0.596
4.8
0.594
100.0
0.5%
4.8
3.0
N 10 10 15.46 21.83 6.37 0.637
0.637
O 10 10 13.44 19.20 5.76 0.576
0.576
0.576
P 10 10 13.46 19.22 5.76 0.576
12%
10
10
14.65
20.74
6.09
0.609
0.619
3.3
0.609
100.0
0.619
3.3
-0.8
R 10 10 16.04 22.53 6.490.649
0.649
S 10 10 16.04 22.10 6.06 p,
0606
0.612
T 10 10 16.31 21.43 6.12 0.612
24%
U
10
10
15.59
21.61
6.02
0.602
0.550
1
11.6
0.602
100.0
0.550
11.6
10.5
V 10 10 16.24 21.80 5.56 0.556
0.556
W 10 10 16.89 21.47 4.59 0.458
0.458
X 10 10 16.35 22.18 5.83 0.583
0.583
Outfall 001: MSD - Minimum Significant Difference
Dunnett's MSD value: 0.0746 PMSD - Percent Minimum Significant Difference
PMSD: 12.1 PMSD is a measure of teat precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) - 12%.
Upper PMSD bound determined by USEPA (90th percentile) = 301%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001 a; USEPA,
2001 b).
USEPA. 200la, 2001 b. Final Report: Interlaborstory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 -Appendix. EPA -821-B-01-004 and EPA -821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
File: btwnsville_050515pp.xls
Entered by:Symner
Reviewed by: iVjf/_

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3 ETS
Environmental T"Wq Solutions, Inc.
Auxiliary Tests
Statistic
Critical
Larval Fish Growth and Survival
Tat -7 Day
Growth
Start Date:
5/5/2015
-0.73
Test ID: PpFRCR
Sample ID:
Burnsville WWTP
End Date:
5/12/2015
Min
Lab ID: ETS-Envir. Testing Sol.
Sample Type:
DMR -Discharge Monitoring Report
Sample Date:
Critical
MSD
Protocol: FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas
Comments:
0.6143
1.0000
0.6143
0.5260
0.6790
Conc-%
1
2
3 4
0.6226
D -Control
0.5260
0.6000
0.6520 0.6790
0.6310
0.5840
1.5
0.6140
0.5840
0.6750 0.6510
2.410
0.0746
3
0.5690
0.5780
0.5680 0.5770
0.9328
0.5730
6
0.5940
0.6370
0.5760 0.5760
1.332
2.410
12
0.6090
0.6490
0.6060 0.6120
0.5958
0.9699
24
0.6020
0.5560
0.4580 0.5830
4
0.597
Auxiliary Tests
Statistic
Critical
Skew
Transform: Untransformed
Shapiro-Wilk's Test indicates normal distribution (p > 0.01)
0.92981
1 -Tailed
-0.73
laotonle
Cone-%
Mean
N-M"n
Mean
Min
Max
CV%
N
tStst
Critical
MSD
Mean
N -Mean
D -Control
0.6143
1.0000
0.6143
0.5260
0.6790
10.965
4
0.6226
1.0000
1.5
0.6310
1.0273
0.6310
0.5840
0.6750
6.362
4
-0.541
2.410
0.0746
0.6226
1.0000
3
0.5730
0.9328
0.5730
0.5680
0.5780
0.912
4
1.332
2.410
0.0746
0.5959
0.9571
6
0.5958
0.9699
0.5958
0.5760
0.6370
4.831
4
0.597
2.410
0.0746
0.5959
0.9571
12
0.6190
1.0077
0.6190
0.6060
0.6490
3.255
4
-0.153
2.410
0.0746
0.5959
0.9571
24
0.5498
0.8950
0.5498
0.4580
0.6020
11.644
4
2.083
2.410
0.0746
0.5498
0.8830
Auxiliary Tests
Statistic
Critical
Skew
Kurt
Shapiro-Wilk's Test indicates normal distribution (p > 0.01)
0.92981
0.884
-0.73
0.89558
Bartletra Test indicates equal variances (p = 0.02)
13.8018
15.0863
Hypothesis Test (1 -fail, 0.05) NOEC LOEC ChV
TU MSDu MSDp
MSB
MSE F-Prob
dr
Dunnetrs Test 24 >24
4.16667 0.07463 0.12149
0.0038
0.00192 0.13057
5.18
Treatments vs D -Control
Linear Interpolation (200 Resamples)
Point % SD 95%CL(Exp) Skew
IC05 13.150
IC10 21.242
IC15 >24
1.0
IC20 >24
0.9
IC25 >24
IC40 >24
0.8
IC50 >24
0.7
0.8
0.7
0.6
z
0.5
E
M 0.4
90.3
0.2
0.1
! 0.6
C 0.5
ffi 0.4
IX 0.3
0.2
0.1
0.0
-0.1 ilyl* -
0 10 20 30
Dose %
Dose -Response Plot
1 -tail, 0.05 level
01 significance
File: bumsville_0505I5ppx13
Entered by: !. S er
Reviewed by: -T

•
0
Envlmnm nt Testl g Solutions. Inc.
Species: Pimephales promelas
Client: Burnsville WWTP
Daily Chemic
Page 4 of 5
Date: 05-05-15
Initial Final Initial Final Initial Final
*Temperatures performed at the time of test initiation., renewal or termination by the analyst identified in the Daily Renewal Information table located
on Page I Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and
transcribed to this bench sheet by: A—.
SOP AT20 — Exhibit AT20.3, revision 11-01-14
Day
(Analyst identified for each day, performed pH,
D.O. and conductivity measurements
only.)
0
1
2
Analyst
LU
Concentration
Parameter
CONTROL
pH (S.U.)
12-
1,24
(�,q
DO (m L)
p
QI
Conductivity
mhos/cm)
*Alkalinity
(mg CaCO3R-)
*Hardness
(mg CaC031L)
*Temperature (°C)
't •d •'1
—L`� • S
.� .
J� .b
"1 �1
Z`l . `�
x (S.U.)
2q1
1.5%
DO (m L)
,�
•�
5.0
Conductivity
(mhos/cm)
Lo IIS�
�T1
*Temperature (°C)
IAA
1- b
'LAA
�.�. S
1-1•">r
1'�.l0
H (S.U.)
-4.3;
.cam}
•2
(p.A3
3.0%
DO (m
q _
•'�
6•0
(p }
Conductivity
(mhos/cm)
1100
ilA
*Temperature (°C)
-L
'Z� .
-1.`�.c1
S
%,•
1� -b
H (S.U.)
-4, Lis'�
2
lo. 2
.2
V•6�1
6..0%
DO (m )
1.9
,O
Conductivity
(mhos/cm)
1-
*Tem erature (°C)
�-`� •
i.'�•b
Z`i•9
i.'�• S
- •
'Ly•3
H (S.U.)
�, 22
qq.2Zto
•'�9
12%
DO (m )
Q
c7
dp
8.0
tP
Conductivity
mhos/cm)
I -12
112 -
�
*Temperature (°C)
—Lk •k
ZS • 0
1-a•1
•�•
Z�1.3
x (&U.)
DO (m L)
O
6•0
(ps
24%
Conductivity
(mhos/cm)
Q
-I Z
,q
2
*Temperature (°C)
"L
'�y.'1
1S •O
1`�•s
Z
�, .S
H (S.U.)
.16
DO (m )
19.0
100%
Conductivity
( mhos/cm)
31g
3�8
*Alkalinity
(mg CaCO3/L)
.� Z
yb
*Hardness
(m CaCO3/L)
3 p
O
(oJ
*TR chlorine (mg/L)
Q . t0
G t 10
*Temperature (°C) -H-It
Initial Final Initial Final Initial Final
*Temperatures performed at the time of test initiation., renewal or termination by the analyst identified in the Daily Renewal Information table located
on Page I Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and
transcribed to this bench sheet by: A—.
SOP AT20 — Exhibit AT20.3, revision 11-01-14

JETS
Envimonmental Tertin Soolution,,Inc.
Species: Pimephales promelas
Client: Burnsville WWTP
Page 5 of 5
Date: 05-05-15
t, lU un,c U1 toNt illuauun, rcncwal or termmauon oy the analyst Identltied in the Daily Renewal Information table located on Page 1.
Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet
by. --
SOP AT20 — Exhibit AT20.3, revision 1 1-01-14

•
� 351 Depot Street
}' Asheville, NC 28801
Phone: [phone]
JIMR — ' Fax: [phone]
Environmental Testing Solutions, Inc.
Whole Effluent Toxicity Chain -of -Custody Form
Facility: Water Quality NPDES #: NCO020290 Pipe #: 001 County: Yancey
Burnsville WWTP Purchase order:
Species: Pimephales promelas Effluent dilution: 1.5, 3.0, 6.0, 12, 24%
Test type: Chronic Definitive Parameter code: THP6C
Sample information: (to be completed by sample collector)
Composite sample: Sample location: Eft, &L4 -
Start date: S�3�is✓ Time: 9: IS.4^ Volume collected for testing: �"l f�iL.'�erS
End date: $/9/t s Time: 304r Number of containers filled for testing: 0AL
Number of samples per hour: 11 Method of transport to laboratory: Co, e; tr
Chilled during collection? YCS Comments:
If chilled, specify temperature: $ C
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.0eC upon receipt at the laboratory.
Sample custody:
Samnle cniiected hv-
(to be completed by sample collector and facility personnel)
t-t'k 3z� g: 30.4
Relinauished bv:
16rk-
`
zoo
Relinquished by:
Print signWue Due nd tme
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by:
16SO'LOS
N 00
Pm sisft Due and tnn
Custody seals intact?:
Yea No Na used
Samples received in good condition?:
Yes No
Tracking number. 0 (r -
Comments:
Received st F.TC h-
•-•• .,q.�au�c ue am ueae
Sample temperature upon receipt at ETS (OC):
Total residual chlorine upon receipt at ETS:
(DPD Presence/Absence Indicator, MDL = 0.10 mg/L) Paso Atsw
Project number: I Sample number: (q)"•V f
s
13W
•-•• .,q.�au�c ue am ueae
Sample temperature upon receipt at ETS (OC):
Total residual chlorine upon receipt at ETS:
(DPD Presence/Absence Indicator, MDL = 0.10 mg/L) Paso Atsw
Project number: I Sample number: (q)"•V f

351 Depot Street
t Asheville, NC 28801
n: Phone: [phone]
- - Fax: [phone]
Environmental Testing Solutions, Inc.
Whole Effluent Toxicity Chain-of-Custodv Form
Facility: Water Quality NPDES #: NCO020290 Pipe #: 001 County: Yancey
Burnsville WWTP Purchase order:
Species: Pimephalespromelas Effluent dilution: 1.5, 3.0, 6.0, 12, 24%
Test type: Chronic Definitive Parameter code: THP6C
Sample information: (to be completed by sample collector)
Composite sample:
Start date: /r Time: /0 W 4.%
End date: SWIr Time: 16: 10 4r
Number of samples per hour: If
Chilled during collection? keg
If chilled, specify temperature: ',° '-
Triple rinse sample container with sample before filling.
Sample location: 9if1ytr4•+
Volume collected for testing: 10 1
Number of containers filled for testing: Owt
Method of transport to laboratory: (,c,N ;t j
Comments:
Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.08C upon receipt at the laboratory.
Sample custody:
Sample collected by:
(to be completed by sample collector and tacility personnel)
1 QIei.��r
I u�lw-0
v I
Riot
Relinquished by:
sipuuve
Dam and time
Riot
Relinquished by:
signature
Due end time
Rent
Signs....
Due and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by:
b
J N � JJ -33
Rim I signuute Due and time
Custody seals intact?: = =
Ya No Na used
Samples received in good condition?: = =
Ya No
Tracking number:
Comments:
lt�
Received by:
5n
sites Die ad time
Received by:
Ptv4 Spa " Dam and time
Received at ETS by:
LZ �" 123-3
mgnuute Due and time
Sample temperature upon receipt at ETS CC):
Total residual chlorine upon receipt at ETS: d
(DPD Presence/Absence Indicator, MDL = 0.10 mg/L) P-- nmm
Project number: /15e6 Sample number: !W40, 3'/

low
Environmental Testing Solutions, Inc.
Whole Effluent Tozi
Chain-of-Custodv Form
351 Depot Street
Asheville, NC 28801
Phone: [phone]
Fax: [phone]
Facility: Water Quality NPDES #: NCO020290 Pipe #: 001 County: Yancey
Burnsville WWTP Purchase order:
Species: Pimephales promelas Effluent dilution: 1.5,3.0,6.0,12,24%
Test type: Chronic Definitive Parameter code: THP6C
Sample information: (to be completed by sample collector)
Composite sampI :
Start date: s ��� Time: 30
End date: u� i► (S' Time: !TD 3 0
Number of samples per hour: '1
Chi
If c
Sample location: FART e., f
Volume collected for testing: 1 pip
Number of containers filled for testing: pl t
Method of transport to laboratory: [,' „r�, w
lled during collection? k Q3 Comments:
billed, specify temperature: ff'
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory.
Sample custody:
Sample collected by:
(to be completed by sample collector and tacility personnel)
I Cor Kl4S-&C--J
.34
Prot sign—
Relinquished by:
Date and time
Prim signatae
Relinquished by:
Date atd time
Prim signaeue
Date and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by:
0
Prot $igoauue Dae etd finte
Custody seals intact?: = =
Ya No Na and
Samples received in good condition?: =
Ya No
Tracking number: [`y {'
Comments:
Received by:
Print sigoanae Due and time
Received by:
Print SW— Dae and time
Received at ETS by:
b4— Due and din
Sample temperature upon receipt at ETS (°C):
Total residual chlorine upon receipt at ETS: r-7 r7l
(DPD Presence/Absense Indicator, MDL = 0.10 mg/L) Pmoo' '�"t
Project number:106(rl/Sample number: ��14�

•
Environmental Testing Solutions, Inc.
1.0
0.9
0.8
0.7
0.6
0.5
1.4
1.2
U
Y 1.0
00
N 0.8
N
U
?, 0.6
ev
.o
0.4
1.4
1.2
1.0
0.8
0.6
0.4
Pimephales promelas
Chronic Reference Toxicant Control Chart
Organism Source: Aquatox, Inc.
USEPA Control Limits (± 2 Standard Deviations)
USEPA Warning and Control Limits (75th and 90th Percentile CVs)
.................................................
........................................................................................................
Laboratory Warning and Control Limits (10th and 25th Percentile CVs)
o3'�Syop'��d�lds'�lcf�1d'"�'Scb'��6i�1�1cf��1Cl�"" 1'1�1'1ti�SyZ�,p�� p111,dLA�,di.��pA����15
Test date
---�— 7 -day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride
that would cause a 25% reduction in Pimephales growth for the test population.
— — Central Tendency (mean IC25)
— — - Warning Limits (mean IC25 ± SA.10 or SA.75)
Graphs generated from
1100clatodemispnedsheet.
......... Control Limits (mean IC25± 5 , S or 2 Standard Deviations Exulspreadsheet• ! J.Sumner
A.25 A.90� ) Reviewed bv: