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HomeMy WebLinkAboutNC0004987_Assessment of Balanced & Indigenous Populations 2014_20141015 ASSESSMENT OF BALANCED AND INDIGENOUS POPULATIONS IN LAKE NORMAN NEAR MARSHALL STEAM STATION NPDES Permit No. NC0004987 Principal Investigators: Michael A.Abney John E. Derwort William J. Foris DUKE ENERGY Environmental Services McGuire Environmental Center 13339 Hagers Ferry Road Huntersville,NC 28078 October 2014 ACKNOWLEDGMENTS The authors wish to express their gratitude to a number of individuals who made significant contributions to this report. First, we are much indebted to the Environmental Monitoring field staff in carrying out a complex, multiple-discipline sampling effort that provides the underpinning of this report. Glenn Long, David Horne, and Courtney Flowe contributed to water quality sample collections. Mark Auten, Kim Baker, Bob Doby, and David Coughlan contributed to fisheries collections and sample processing. James Hall, Aileen Lockhart, Shannon McCorkle, and Jan Williams contributed in macroinvertebrate sampling, sorting and taxonomic processing. We would also like to thank multiple reviewers including Jason Brown and Tom Thompson. ii TABLE OF CONTENTS EXECUTIVE SUMMARY iv LIST OF TABLES vi LIST OF FIGURES vii CHAPTER 1- INTRODUCTION 1-1 CHAPTER 2- STATION OPERATION 2-1 BACKGROUND INFORMATION 2-1 THERMAL DATA 2-2 CONCLUSIONS 2-3 CHAPTER 3- MACROINVERTEBRATES 3-1 MATERIALS AND METHODS 3-1 RESULTS AND DISCUSSION 3-2 Substrate 3-2 Water Quality 3-2 Taxa 3-2 Density 3-3 Major Taxonomic Groups 3-3 EPT Densities and Taxa Richness 3-5 CONCLUSIONS 3-6 CHAPTER 4- FISH 4-1 MATERIALS AND METHODS 4-1 Spring Electrofishing Surveys 4-1 Summer Electrofishing Surveys 4-1 Fall Hydroacoustic and Purse Seine Surveys 4-2 Balanced and Indigenous Assessment 4-2 RESULTS AND DISCUSSION 4-3 Spring Electrofishing Surveys 4-3 Summer Electrofishing Surveys 4-4 Fall Hydroacoustic and Purse Seine Surveys 4-5 CONCLUSIONS 4-5 LITERATURE CITED L-1 iii EXECUTIVE SUMMARY Annual monitoring of physicochemical characteristics and assessments of macroinvertebrate and fish populations at selected locations in Lake Norman continued through 2013, in accordance with Duke Energy's agreement with the North Carolina Department of Environment and Natural Resources. This report presents data collected from 2009 through 2013. Results of data analyses completed since submittal of the previous report in 2008 are reported and support renewal of the existing permitted thermal limits for Marshall Steam Station(MSS). The continuous operation of MSS from 2009 through 2013 was similar to previous years. Monthly average discharge water temperatures at MSS were in compliance with National Pollution Discharge Elimination System (NPDES) permitted thermal limits of 93.9 °F (34.4 °C) from July 1 through October 31, and 91.9 °F (33.3 °C) the rest of each year, over this five-year period. Sampling of macroinvertebrate communities continued during 2009 — 2013. Water temperatures and dissolved oxygen levels, measured at each location since 2005, do not suggest any negative impact to the benthic communities. Macroinvertebrate data exhibit substantial variability; however, the variability is consistent with that observed for historical data. Although taxa numbers were somewhat lower during 2009—2013 as compared to 2004 — 2008, macroinvertebrate densities were generally similar between the two study periods. Current study results indicate that, overall, the macroinvertebrate densities and taxa diversity observed during 2009 — 2013 at locations uplake, downlake, and in the vicinity of MSS are indicative of balanced and indigenous macroinvertebrate populations. A diverse fish community was present in the littoral surveys of Lake Norman near MSS from 2009 to 2013. Spring and summer electrofishing surveys documented 28 and 19 species, respectively, both numerically dominated by centrarchids, especially bluegill. Pollution- tolerant species comprised less of the spring MSS fish population relative to the reference area, and comprised only 16.6% of individuals collected during summer surveys. The assorted fish species typically found in Lake Norman near MSS encompass multiple trophic guilds (i.e., insectivores, omnivores, and piscivores) supporting a balanced fish community. Non-indigenous species such as spotted bass and green sunfish are abundant near MSS, but are also prevalent throughout Lake Norman. iv Annual hydroacoustic estimates from fall 2009 to 2013 showed the regular availability of pelagic forage fish near MSS. Fall purse seine surveys indicate that threadfin shad continue to dominate the Lake Norman forage fish community with a consistent alewife composition of approximately 5% after 2004. Based on the catch per unit effort of littoral fish during spring, total length distributions of resident important species during summer, and the regular availability of forage fish, it is concluded that the thermal discharge of MSS has not impaired the Lake Norman fish community. Comparison of MSS operation and environmental monitoring data indicate that balanced and indigenous populations of macroinvertebrates and fish continue to exist in Lake Norman in the vicinity of MSS. This supports a conclusion that the present thermal limits should be maintained when the MSS NPDES permit is renewed. v LIST OF TABLES Table Title Page 1-1 Description of Lake Norman sampling locations in the vicinity of the MSS, Catawba County,NC. 1-2 2-1 Marshall Steam Station CCW flow rate for each unit for 1, 2, and 3-pump operation 2-4 3-1 General descriptions of the substrate found at Locations A,B,F, and E in the vicinity of MSS during July of 2009—2013. Substrates are listed in order of the most prevalent type first. Organic matter(om)is typically composed of small sticks, leaf and/or grass fragments, etc 3-8 3-2 Dissolved oxygen and temperature measured near the sediments at the time of macroinvertebrate collection from locations A, B, F, and E from July 2009— 2013 3-8 3-3 Macroinvertebrate taxa and densities (No./m2) from each Lake Norman sampling location during summer sampling in 2009. 3-9 3-4 Macroinvertebrate taxa and densities(No./m2)at each Lake Norman sampling location during summer sampling in 2010 3-11 3-5 Macroinvertebrate taxa and densities(No./m2)at each Lake Norman sampling location during summer sampling in 2011. 3-13 3-6 Macroinvertebrate taxa and densities (No./m2) at each Lake Norman sampling location in during summer sampling in 2012 3-15 3-7 Macroinvertebrate taxa and densities (No./m2) at each sampling location in Lake Norman during summer sampling in 2013 3-17 4-1 Total number of individuals,percent composition, and total number of species in spring electrofishing surveys from two areas(MSS and REF)in Lake Norman, 1993 — 1997 and 1999, 2000—2003, 2004—2008, and 2009—2013. 4-6 4-2 Total number of individuals,percent composition, and total number of species in summer electrofishing surveys from three areas near MSS (above,in the vicinity of, and below the discharge canal)in Lake Norman, 1991 — 1993, 1994— 1999, 2000—2003, 2004—2008, and 2009—2013. 4-7 4-3 Pelagic forage fish species composition from purse seine surveys in Lake Norman, 1993 —2013 4-8 vi LIST OF FIGURES Figure Title Page 1-1 Sampling locations on Lake Norman. Duke Energy historical sampling location numbers are listed in parentheses 1-3 2-1 The monthly average water temperature of the condenser cooling water discharged from MSS from January 1,2009 through December 31, 2013 2-5 3-1 Total number of taxa collected annually from Lake Norman in the vicinity of MSS from 2009 to 2013 3-19 3-2 Densities (No./m2)of macroinvertebrates collected annually from Lake Norman in the vicinity of MSS from 2009 to 2013. 3-19 3-3 Densities(No./m2)of Oligochaeta collected annually from Lake Norman in the vicinity of MSS from 2009 to 2013. 3-20 3-4 Densities(No./m2)of Chironomidae collected annually from Lake Norman in the vicinity of MSS from 2009 to 2013. 3-20 3-5 Density(No./m2)of Oligochaeta, Diptera, and Corbicula collected annually from Location F (mid-lake near MSS) from 2000—2008 3-21 3-6 Densities (No./m2)of Corbicula collected annually from Lake Norman in the vicinity of MSS from 2009 to 2013. 3-21 3-7 Densities(No./m2) of Ephemeroptera collected annually from Lake Norman in the vicinity of MSS from 2009 to 2013. 3-22 3-8 Densities(No./m2)of Trichoptera collected annually from Lake Norman in the vicinity of MSS from 2009 to 2013. 3-22 3-9 Densities (No./m2)of Megaloptera from Lake Norman in the vicinity of MSS from 2009 to 2013 3-23 3-10 Annual densities(No./m2)of Spheariidae from Lake Norman in the vicinity of MSS from 2009 to 2013. 3-23 3-11 Annual densities of Ephemeroptera,Plecoptera, and Tricoptera(EPT in no./m2) from Lake Norman in the vicinity of MSS from 2009 to 2013. 3-24 3-12 Annual Ephemeropteran/Plecopteran/Trichoptern(EPT)richness from Lake Norman in the vicinity of MSS from 2009 to 2013. 3-24 4-1 Locations associated with spring electrofishing and fall purse seine surveys, and zones associated with fall hydroacoustic survey of Lake Norman. 4-9 4-2. Total number of fish collected in spring electrofishing surveys from two areas (MSS and REF)in Lake Norman, 1993 — 1997 and 1999—2013. 4-10 4-3 Biomass of fish collected in spring electrofishing surveys from two areas(MSS and REF) in Lake Norman, 1993 — 1997 and 1999—2013. 4-10 4-4 Total number of fish species collected in spring electrofishing surveys from two areas(MSS and REF)in Lake Norman, 1993 — 1997 and 1999—2013 4-11 4-5 Length distribution of bluegill among survey locations in summer electrofishing surveys near MSS in Lake Norman, 2009—2013. 4-11 vii LIST OF FIGURES (continued) Figure Title Page 4-6 Length distribution of redbreast sunfish among survey locations in summer electrofishing surveys near MSS in Lake Norman, 2009—2013. 4-12 4-7 Length distribution of spotted bass among survey locations in summer electrofishing surveys near MSS in Lake Norman, 2009—2013. 4-12 4-8 Pelagic forage fish density estimates by zone in Lake Norman, late summer/early fall 1997—2013. 4-13 viii CHAPTER 1 INTRODUCTION Annual monitoring of physicochemical characteristics and assessments of macroinvertebrate and fish populations at selected locations in Lake Norman continued from January 2009 through December 2013 (Table 1-1 and Figure 1-1), in accordance with Duke Energy's agreement with the North Carolina Department of Environment and Natural Resources (NCDENR). Physicochemical and biological data were collected at locations near Marshall Steam Station (MSS) and included a location upstream of the facility (Location A), two sites within the immediate projected impact of MSS's discharge (Locations B and C) and three downstream recovery sites (Locations D, E, and F), presumably outside the influence of the effluent plume(Figure 1-1). The objectives of this on-going monitoring program are to provide an assessment of the balanced and indigenous nature of the biological populations in Lake Norman with respect to the thermal discharge from MSS and evaluate renewal of thermal limits at the station. The thermal limit for MSS is a monthly average discharge (i.e., end-of-pipe) temperature limit of 93.9 °F (34.4 °C) from July 1 through October 31, and 91.9 °F (33.3 °C) the rest of each year. Regulatory review of past studies has determined, pursuant to Section 316(a) of the Clean Water Act, that the thermal discharge of MSS ensures the protection and propagation of balanced, indigenous populations in Lake Norman. This report presents data collected since submittal of the previous summary reports (Duke Power Company 1994; Duke Power 1999, 2004a; Duke Energy 2009a) and includes data collected from 2009 through 2013. These data were also compared with other past and present on-going environmental monitoring programs conducted in this watershed. 1-1 Table 1-1. Description of Lake Norman sampling locations in the vicinity of MSS, Catawba County,NC. Approximate River Miles Report Upstream of Location Duke Energy Cowans Ford Designation Location No. Location Description County Hydro Longitude Latitude The first cove on the left upstream from MSS A 15.5 intake cove. Catawba 16.9 -80.9461 35.6163 First cove along northern shoreline going into the B 14.7 MSS CCW discharge cove. Catawba 13.9 -80.9573 35.5942 C 14.5 Inside MSS discharge canal. Catawba 13.9 -80.9644 35.5951 Large cove halfway between channel markers 14 D 13.0 and 15 on the west side of the channel. Catawba 12.7 -80.9527 35.5753 Cove formerly proposed for power plant site E 19.0 intake or discharge. Iredell 9.0 -80.9301 35.5297 Left shoreline, approximately 100 m uplake of F 34.0 Channel Marker 13. Catawba 11.5 -80.9569 35.5544 N Lookout Shoals Hydro ' A V " Key • Monitoring locations a }A (15.5 ,, Marshall • a Steam Atil Station - ' C (14.5) 8 (14. ) Miles D (13.0) 1 2 3 Kilometers 0 2 4 F (34.0) , E (19.0 ` a • O t3 a • Cowans Ford Hydro ■ McGuire Nuclear Station Figure 1-1. Sampling locations on Lake Norman. Duke Energy historical sampling location numbers are provided in parentheses. 1-3 CHAPTER 2 STATION OPERATION BACKGROUND INFORMATION Marshall Steam Station (MSS) is located on the western shore of Lake Norman (Figure 1-1) just north of Charlotte, NC, in Catawba County. Its four generating units have a combined operating capability of 2,090 MWE-net. Units 1 and 2 are each rated at 385 MWE-net and began commercial operation in 1965 and 1966, respectively. Units 3 and 4 are each rated at 660 MWE-net and began commercial operation in 1969 and 1970,respectively. MSS receives once-through condenser cooling water (CCW) from below a skimmer wall located at the mouth of a 1.3-mi- (2.1-km-) long cove. The surface area of the cove is about 200 ac (81 ha) and its volume is approximately 250 million ft3(7 million m3). The skimmer wall was designed to retain the upper 60 ft (18.3 m) of water on the lake side of the wall at full pond. The opening below the skimmer wall through which the station CCW is withdrawn is about 10 ft(3 m)high and 270 ft(82.3 m)wide. Units 1 and 2 each have two condenser cooling water pumps, and Units 3 and 4 each have three condenser cooling water pumps. Typically, only one pump per generating unit is used during the cool winter months or when the unit is operating at reduced load. During the warmer summer months when units are operating at full-load, either two or three pumps per unit are generally used, depending on which units are operating. Maximum rates of cooling water flow are 423 cfs (12.0 cms) each for Units 1 and 2, and 709 cfs (20.1 cms) each for Units 3 and 4. Thus, the maximum possible station CCW flow rate is 2,264 cfs (64.1 cms). Under one-pump-per-unit operation, the maximum CCW flow rate for the entire station is 1,230 cfs (34.8 cms). Refer to Table 2-1 for a listing of CCW flow rates for each unit under one-, two-, and three-pump operation. The CCW from MSS is discharged into a one-mile- (1.609 m-) long cove. The discharge cove varies from a width of 75 ft (22.9 m) and depth of 33 ft (10.1 m) at the discharge structure to a width of 850 ft (259.1 m) and a depth of 50 ft (15.2 m) at the mouth of the discharge cove. The total area of the discharge cove is about 75 ac(30.4 ha). 2-1 Lake Norman was recently classified as oligotrophic, or of low nutrient content and algal productivity, based on year 2012 water quality monitoring performed by the North Carolina Department of Environment and Natural Resource (NCDENR) in association with the Lake Assessment Program, which is conducted on a five-year cycle (NCDENR 2013). This classification is consistent with earlier assessments (NCDENR 2003, 2008) indicating that despite increases in population growth in the upper Catawba River Basin over the last two decades, Lake Norman continues to effectively assimilate nutrient inputs into the reservoir from the surrounding watershed. Nutrient and algal biomass data, collected monthly from May through September 2012, ranged from low to moderate levels, with higher values reported in the upper portions of the reservoir, combined with progressively decreasing concentrations in the lower sections of the reservoir (NCDENR 2013). Turbidity levels were also low and Secchi depths ranged from 1.0 to 4.0 m, indicating very good water clarity. Surface dissolved oxygen (DO) concentrations ranged from 5.8 to 8.8 mg/L and surface water temperatures ranged from 76.1 °F to 94.28 °F (24.5 to 34.6 °C). Overall, these results are similar to those previously reported by the State (NCDENR 2003, 2008) and to other studies (MCDEP 2003, 2007; Duke Power Company 1994; Duke Power 1999, 2004a; Duke Energy 2008, 2009d, 2011, 2012, 2013) on Lake Norman. THERMAL DATA Duke Energy operates MSS as a base-load generating facility and station operation during the period from January 2009 through December 2013 was similar to previous years (Duke Power Company 1994; Duke Power 1999, 2004a; Duke Energy 2009a). From 2009 through 2013, MSS was operated continuously with peak pumping of CCW during the summer. The seasonal cycle of discharge water temperatures at MSS over the period January 2009 through December 2013 was also similar to that observed in previous years (Figure 2-1; Duke Power Company 1994; Duke Power 1999, 2004a; Duke Energy 2009a). Monthly average discharge water temperatures at MSS were in compliance with NPDES-permitted thermal limits of 93.9 °F(34.4 °C) from July 1 through October 31, and 91.9 °F (33.3 °C)the rest of each year, over this five-year time period (Figure 2-1). Discharge water temperatures ranged from a minimum monthly average of 62.4 °F (16.9 °C) in February 2011 to a 2-2 maximum monthly average of 93.9 °F (34.4°C) in August 2011 (Figure 2-1). Discharge temperatures are linked to a combination of local meteorological conditions and electrical generation. CONCLUSIONS MSS operated continuously from 2009 through 2013 and station operation during this period was similar to previous years. Monthly average discharge water temperatures at MSS were in compliance with NPDES-permitted thermal limits of 93.9 °F (34.4 °C) from July 1 through October 31, and 91.9 °F (33.3 °C)the rest of each year,over this five-year period. 2-3 Table 2-1. Marshall Steam Station CCW flow rate for each unit for one-,two-, and three- pump operation. Unit One-Pump CCW Two-Pump CCW Three-Pump CCW Number Flow Rate(cfs) Flow Rate(cfs) Flow Ratelcfs) 1 281 423 (423) 2 281 423 (423)1 3 334 564 709 4 334 564 709 Station Total 1,230 1,974 2,264 1 Units 1 and 2 have only two CCW pumps available. 2-4 0, 0 0 N (0 seeZaa) anwadwai E , i , 1 , , I ,i, , , I i . , , II, i Vi'Ller c. I . , i i 0 I i i 1 1 0 . c oiew TI141;41148814;44.1&. ! I I c lied 78 1 1 I OD I I I Z -AON ,4 c.) - cn - ' z i-das -0— 4.4...1.,;\ n - _l_ 1 t I Zi-urif VI I ZI-AV ta , Z I-uer 75 ; 0 . II-130 r) .. 0 11-6nV 0 • a) -0 g U." . 14-AelAl co 0 0 _ Ct •- 1.lleJ -0 . (,-- : 0 L-Dea o , 1 0 1-daS 0 .g co t ! 0 1-Inr a, , 0 1-ichl g , t ,..• 1 0 v• i 0 Vuer r<Z4.......4 , I&) g 1 114 a" N - 01 Op 60-no N - OA en 60nV-Oel. t t › .. ., 60-Aell al 5 . - >, u i c.) - 60-ie IN g 4) m 3 0 a attitata 111 + 111 III § III . • II IIIIiIIII Ilia c' 1) (d seei6e0) einieJedwei A cu a .. w 2-5 CHAPTER 3 MACROINVERTEBRATES MATERIALS AND METHODS Benthic macroinvertebrate sampling was conducted annually in the summer of each year from 2009 to 2013 as part of the continuing monitoring program for Marshall Steam Station (MSS). As in previous years, samples were collected from four locations on Lake Norman: Location A (uplake of MSS), Location B (MSS discharge canal), Location F (just north of Channel Marker 13), and Location E (downlake of MSS) (Table 1-1 and Figure 1-1). A petite Ponar dredge (15.3 x 15.3 cm) was used to collect five sample replicates at each location. Samples were collected at depths ranging from 2 to 3 m to bracket the depth of peak benthic abundance commonly associated with lakes and reservoirs (Brinkhurst 1974). Samples were washed through a 500-µm mesh sieve and individually preserved with 70% ethanol containing rose bengal stain. The substrate at each location was visually identified and recorded during the sieving process. Organisms were sorted in the laboratory and identified to the lowest practicable taxon. Macroinvertebrate densities were determined from each replicate, averaged and expressed in No./m2. The NCDENR requested additional analyses to be performed based on their review and response to the 2009 MSS report (Duke Energy 2009a; NCDENR letter of November 19, 2009). In addition to current analyses, NCDENR requested additional information on the following groups: Non-chironomid Diptera, Ephemeroptera, Plecoptera, Trichoptera, Coleoptera, Megaloptera, Odonata, Crustacea, and Mollusca. Ephemeroptera/Plecoptera/Trichoptera (EPT) densities and taxa richness were also requested for additional assessment of the balanced and indigenous nature of the benthic community in the vicinity of MSS. No Coleoptera, or Crustacea have been collected at locations in the vicinity of MSS in the last 10 years and Plecoptera were only collected once during 2006 (Duke Energy 2009a). The only Mollusca collected were represented by Corbicula and Spheariidae,which are addressed separately in this report. 3-1 Beginning in 2005, in conjunction with macroinvertebrate sampling, a calibrated YSI Model 55 handheld DO meter was used to measure water temperature and DO just above the sediment at each of the four sampling locations. Starting in 2008, water temperatures and DO concentrations were measured, in situ, using a calibrated Hach® HQ40d water quality meter. RESULTS AND DISCUSSION Substrate Substrates at Lake Norman sampling locations consisted of varying amounts of silt, sand, organic material, and clay. Silt was the predominant component at most locations, while comparatively high amounts of clay and sand were often observed in the substrates at Location E (Table 3-1). Water Quality Water temperatures observed during sampling from 2009 to 2013 ranged from 26.4 to 31.1 °C (Table 3-2). No consistent spatial patterns among maximum and minimum temperatures were observed during 2009—2013 and temperatures at Location B in the discharge were not notably different from those at other sampling locations. The DO concentrations observed during 2009 —2013 ranged from 1.0 to 8.7 mg/L (Table 3- 2). Minimum DO concentrations were most often recorded from Location B in the discharge, while maximum DO readings were most often observed at Location A. The comparatively high DO recorded in 2013 may have been due to the unusually cool and wet summer of that year, with a much higher level of water column mixing. Additionally, with the cool, wet conditions, MSS may have minimized operations. Minimum DO concentrations at the discharge were likely due to low-DO water from beneath the skimmer wall being entrained through MSS during summer periods of 2009 through 2012. Taxa The number of macroinvertebrate taxa collected at a location is typically a good indicator of the overall diversity and the presence of a balanced indigenous population. Taxa abundance 3-2 from 2009 to 2013 varied temporally and spatially at Lake Norman (Tables 3-3 through 3-7 and Figure 3-1). Taxa abundance during 2009 — 2013 was slightly lower than during the 2004 — 2008 reporting period (Figure 3-1), with total taxa numbers ranging from 18 to 36. Temporally, taxa numbers generally declined from 2009 through 2011, and then increased between 2011 and 2013. Spatial maxima occurred at Location A in 2013, Location B in 2011, and Location F in 2009, 2010, and 2012. Spatial minima occurred at Location E in 2009, 2010, and 2011, and Location B in 2012 and 2013. Density During 2009 - 2013, overall annual macroinvertebrate densities varied substantially temporally and spatially, and total densities were not appreciably different from densities during 2004—2008 (Tables 3-3 through 3-7 and Figure 3-2). Macroinvertebrate densities in Duke Energy's piedmont reservoirs are typically characterized by very high temporal and spatial variability (Duke Energy 2009a, 2009b, 2009c, 2011). This was also true of the densities of major taxonomic groups. Variability may be due to macroinvertebrates and sediments not being homogenously distributed on the substrates. High spatial and temporal variability can often mask short-term or long-term environmental impacts. Total densities ranged nearly an order of magnitude from minimum to maximum during 2009 — 2013. As with total taxa numbers, densities generally declined from 2009 through 2011, and then increased between 2011 and 2013. No consistent trend in spatial maxima was observed. Minimum spatial densities were consistently recorded from Location E well downlake of the MSS discharge(Figure 3-2). This was also the case during the 2004—2008 reporting period. It is unlikely that thermal impacts would be manifested at this location to a greater extent than at other locations since no consistent temporal or spatial temperature patterns were observed (Table 3-2). The predominance of less suitable substrates (clay in 2009 and 2010, sand in 2011 — 2013), as well as substrate patchiness, may have resulted in less suitable conditions for benthic macroinvertebrate colonization and growth at Location E (Table 3-1). Major Taxonomic Groups Considerable variability was also observed among major taxonomic groups. Oligochaeta densities during 2009—2013 varied nearly 50 times from minimum to maximum (Tables 3-3 through 3-7 and Figure 3-3). Oligochaetes were dominant in Lake Norman macroinvertebrate samples approximately 40% of the time. Maximum oligochaeta densities occurred at Location B in 2009, 2012, and 2013, and they were most abundant at Locations 3-3 A and F in 2010 and 2011, respectively. Higher oligochaeta densities at the discharge were indicative of tolerance for the low DO conditions typically observed there. The lowest oligochaeta densities consistently occurred at Location E (Figure 3-3). The majority of oligochaetes at all locations were the Tubificidae. Milligan (1997) stated that Tubificids frequently form dense populations in organically enriched habitats with a silty or muddy substrate tending toward anoxic conditions. Sediment composition at most locations consisted primarily of silt with organic matter and some sand, while at Location E, clay and sand were often among primary components (Table 3-1). Oligochaeta densities showed an overall decline from 2009 through 2011, followed by increases between 2012 and 2013. The family Chironomidae represents a ubiquitous and widespread group of insects that can be found in a broad variety of aquatic habitats. The Chironomidae tended to show temporal and spatial variability at Lake Norman, and densities during 2009 — 2013 were similar to those observed during 2004 — 2008 (Figure 3-4). Densities ranged nearly six fold from minimum to maximum during 2009 — 2013 (Tables 3-3 through 3-7 and Figure 3-4). Chironomids constituted the most abundant taxonomic group at Lake Norman locations, and were dominant in approximately 60% of samples collected during 2009 — 2013. Maximum chironomid densities were observed at Location F in 2009, 2010, and 2012; while maxima at Locations A and B were observed in 2011 and 2013, respectively. Minimum densities were most often recorded from Location E. Non-chironomid dipteran taxa densities showed considerable variability, ranging from 0/m2 up to over 600/m2 (Tables 3-3 through 3-7 and Figure 3-5). In most cases, total densities were less than 50/m2. Densities tended to decline from 2009 through 2012, followed by an increase in 2013. No consistent spatial trends were observed. Mean annual densities of Corbicula during 2009 — 2013 tended to be lower than those recorded during 2004 — 2008 (Figure 3-6). Spatial maxima most often occurred at Location F, while minimum densities were generally recorded from Location B. Data from the previous 10 years show there has been an apparent long-term decline in clam densities since 2008. Densities ranged from 0/m2 to over 1,300/m2 and during most years from 2009 through 2013, Corbicula densities were less than 500/m2(Tables 3-3 through 3-7). Mean annual densities of Ephemeroptera during 2009 — 2013 were generally higher than those of 2004 — 2008 (Figure 3-7), ranging from 0/m2 to nearly 400/m2 (Tables 3-3 through 3-7). The overall trend seemed to indicate increased densities through 2011, followed by a 3-4 decline through 2013. This was the opposite of what was observed among total densities at Lake Norman locations (Figure 3-2). No consistent spatial trends were observed (Figure 3- 7). Mean annual densities of Trichoptera during 2009—2013 were somewhat higher than during the previous five-year sampling period (Figure 3-8). Densities ranged from 0/m2 to over 100/m2 (Tables 3-3 through 3-7). No consistent temporal or spatial trends were observed among Trichoptera during 2009—2013. The presence and abundance of Megaloptera and Spheariidae were sporadic during 2009 — 2013 (Figures 3-9 and 3-10). Megaloptera were only observed at Locations A and E, and densities were always less than 45/m2 (Tables 3-3 through 3-7). Overall densities were slightly higher during 2009 — 2013 than during the previous five-year period (Figure 3-9). No Spheariidae were collected in 2010 or 2011, and overall densities were lower during 2009 — 2013 than during 2004 — 2008 (Figure 3-10). Odonata were observed infrequently during 2004 — 2008 and no odonates were collected in Lake Norman samples during 2009 — 2013 (Tables 3-3 through 3-7). EPT Densities and Taxa Richness Densities of EPT taxa (Ephemeroptera and Trichoptera only, no Plecoptera were collected during 2009—2013) showed slightly higher densities during 2009—2013 than during 2004— 2008 (Figure 3-11). EPT densities ranged up to over 45 fold from minimum to maximum during 2009 — 2013 (Tables 3-3 through 3-7). Generally, densities appeared to increase between 2009 and 2011, and then decline from 2012 to 2013. No consistent spatial trends were observed among EPT densities. The EPT taxa richness values during 2009—2013 were typically higher than during the 2004 — 2008 sampling period (Figure 3-12). Most taxa richness ratings ranged from Fair to Excellent. Excellent EPT richness was observed at Location A in 2010, Location B in 2011, and Location F in 2011 and 2013. Poor richness ratings were recorded from Location B in 2009 and 2012, at Location E in 2010 and 2011, and at Location F in 2012. Low ratings at Location B (the discharge) may have been due to low DO conditions (Table 3-2), while the low ratings at Location E may have resulted from substrate patchiness and occasionally poor substrate conditions. EPT richness ratings appeared to increase substantially from 2009 3-5 through 2011, and then decline at all but Location E in 2012. Ratings increased again through 2013 at all but Location E. CONCLUSIONS Substrates at Lake Norman locations generally consisted of varying proportions of silt, sand, organic matter and clay with somewhat higher composition of clay and sand at Location E. The water quality parameters (temperature and DO) taken at the time of macroinvertebrate collections did not suggest any negative impact to the benthic communities. The only water quality issue may be related to the low DO values at most times of sampling at Location B, which could be expected since MSS withdraws its condenser cooling water from the bottom of Lake Norman via an intake canal skimmer wall (Chapter 2). The common characteristic among macroinvertebrate communities at Lake Norman locations was the high variability among total densities and the densities of major taxonomic groups. This is common at sampling locations on other piedmont reservoirs in North Carolina. Although taxa numbers were somewhat lower during 2009 — 2013 as compared to 2004 — 2008, macroinvertebrate densities were generally similar between the two study periods. Both taxa numbers and densities showed an apparent decline from 2009 through 2011, followed by an increase from 2011 through 2013. No consistent spatial trends in maximum densities were observed. Minimum taxa numbers were recorded at Location E during three of the five years represented, while minimum densities were consistently reported from this location. Chironomids were the most abundant macroinvertebrates at most locations during 2009 — 2013, while oligochaetes constituted the second most abundant forms. The majority of oligochaetes were Tubificids, which often formed dense concentrations in organically enriched habitats. Chironomid maxima were most often recorded from Location F, while maximum oligochaete densities (mostly Tubificids) were most often recorded from Location B in the discharge. Comparatively high oligochaeta densities in the discharge were likely due to their tolerance of the low DO conditions observed there. Minimum densities of both groups typically occurred at Location E. Low total densities, as well as minimum densities of chironomids and oligochaetes at this location, may have been due to less suitable substrate and/or substrate patchiness. 3-6 Non-chironomid Diptera, Corbicula, Ephemeroptera, and Trichoptera densities showed considerable variability and few consistent spatial or temporal patterns were observed during 2009 — 2013. The presences and abundances of Megaloptera and Spheariidae were extremely sporadic during the recent five-year monitoring period, and no odonates were collected during 2009—2013. EPT taxa richness and densities during 2009 — 2013 were typically higher than in 2004 — 2008 and most richness ratings during 2009—2013 were from Fair to Excellent. Poor ratings were observed occasionally from the discharge location and from Location E. This was likely due to low DO conditions in the discharge and possible poor substrate or substrate patchiness at Location E. Some impacts were noted at the MSS discharge location due to low DO conditions; however, based on macroinvertebrate densities, total taxa numbers, and EPT densities and taxa richness observed during 2009 — 2013, it is concluded that thermal discharges from MSS have not impaired macroinvertebrate communities in the vicinity of the station. 3-7 Table 3-1. General descriptions of the substrate found at Locations A, B, F, and E in the vicinity of MSS from July 2009 - 2013. Substrates are listed in order of the most prevalent type first. Organic matter (om) is typically composed of small sticks, leaf and/or grass fragments, etc. Year Location A Location B Location F Location E silt silt silt clay 2009 om clay om silt sand om sand om sand sand silt silt silt silt 2010 om sand om clay sand om sand om silt silt silt silt 2011 sand sand om sand om om sand silt silt om silt 2012 sand sand silt sand om om silt silt silt silt 2013 om om sand sand sand om om Table 3-2. DO and temperature measured near the sediments at the time of macroinvertebrate collection from locations A, B, F, and E from July 2009- 2013. Year Location A Location B Location F Location E 2009 8.3 mg/L 1.6 mg/L 7.1 mg/L 8.2 mg/L 28.1 °C 27.1 °C 27.5 °C 26.4 °C 2010 8.6 mg/L 1.3 mg/L 7.0 mg/L 8.2 mg/L 29.9 °C 31.1 °C 30.2 °C 30.0 °C 2011 7.7 mg/L 1.0 mg/L 7.1 mg/L 7.7 mg/L 28.0 °C 28.2 °C 29.0 °C 29.2 °C 2012 7.5 mg/L 1.8 mg/L 8.1 mg/L 8.0 mg/L 30.1 °C 29.4 °C 30.0 °C 30.8°C 2013 8.7 mg/L 5.3 mg/L 8.2 mg/L 7.9 mg/L 28.7°C 28.6 °C 28.5°C 28.0°C 3-8 Table 3-3. Macroinvertebrate taxa and densities (No./m2) from each Lake Norman sampling location during summer sampling in 2009. 2009 Locations Taxa A B F E Diptera Ceratopogonidae Palpamyia-Bezzia complex TT 17 17 17 Chaoboridae Chaoborus spp. 585 112 26 Chironomus spp. 9 52 Claadgpelma spp. 17 112 Cladotanytarsus spp. 732 17 887 Cryptodhiranamus app. 26 9 138 17 Cryptotendpes spp. 34 362 189 60 Diaotenaihpes neornodestus 26 Nilothauma spp. 9 Pagastiedla spp. 17 77 26 9 Paralauterbomiella nigmhalteralis 9 9 9 Paratendipes spp. 9 Polypedilum halterale gr. 250 17 43 Polypedilum scalaenum gr. 9 9 26 Pseudodriron mus spp. 17 112 Stempellina spp. 26 17 Stenochironomus spp. 17 43 Stictochiranamus spp. 258 121 103 Tanytarsrs spp. 310 60 336 43 Chiron Parakiefferiella spp. 17 Ablabesmyia annulata 9 17 Ablabesmyia mallochi 9 9 Ablabesmyia ramphe gr. 9 Clinotaanypus spp. 172 Coelotanypus spp. 86 172 60 Djalmabatista puldna Prodadius spp. 241 697 112 121 Ephemeroptera Caenidae Caenis spp. 60 Ephemeridae l-lexagenia spp. 26 17 9 Megaloptera Sialidae Sialis spp. 26 Oligochaeta Naidldae 9 60 Dern trifida 9 146 3-9 Table 3-3. (Continued). 2009 Locations Taxa A B F E Oligochaeta Nais spp. 52 34 Nais pardalis 9 26 Tubiicidae 870 1,093 456 155 Aulodrilus limnobius 43 250 138 Autodrilus pigueti 9 4,830 Branchirua sowverbyi 17 Other Glossiphoniidae Helobdella stagnalis 43 422 319 17 Planariidae Cura formanii 9 69 Sabellidae Maneyunkia speciosa 508 818 43 Tetrastemmatidae Prostana graecens 69 Nematoda 86 534 77 Pelecypoda Corbiculidae Corbicula flumina 146 827 284 Sphaeriidae Sphaerium spp. 310 215 Trichoptera Leptoceridae Oecetis spp. 17 17 17 43 Triaenodes igniters 26 Polycentropodidae Nyclophylax spp. 26 Polycentropus spp. 17 17 Total Density for Year 4,022 9,652 5,356 1,550 Total Taxa for Year 27 27 36 24 3-10 Table 3-4. Macroinvertebrate taxa and densities (No./m2)at each Lake Norman sampling location during summer sampling in 2010. 2010 Locations Taxa A B F E Diptera Ceratopogonidae Palpamyra-Bezzia complex 17 17 26 ( omidae-Chironominae Axarus spp. Chiranornus spp. 95 Cladopelma spp. 9 34 Cladotanytarsus spp. 723 17 ;•;7 TT Cryptochiranomus spp. 69 284 69 Cryptotendipes app. 7T 413 336 9 Dicrotendipes neomodestus 60 Nilothauma spp. 9 Pagastiella spp. 103 Paralauterbamiella nigrohalteralis 43 60 Polypedilum halterale gr 43 9 43 26 Polypedilum scalaenum gr 9 77 Stempellina spp. 17 146 Stenochironomus app. 241 43 9 9 Stictochiionomus app. 17 207 Stictochironamus caffranius 9 9 Tanytarsus app. 422 34 189 34 Chiron 17 9 Paraldefferiella spp. 26 Ablabesmyia annulata 9 60 Ablabesmyia ramphe gr 52 17 26 Coelotanypus spp. 43 181 69 Djalmabatista pulchra 9 Parclad►us spp. 155 422 26 138 Ephemeroptera Baetidae Pseudvdoeon app. 86 198 Caenidae Caenis app. 241 Ephemeridae Hexagenia spp. 26 103 9 Megaloptera Sialidae Sialis app. 43 34 Oligochaeta Naididae 9 69 Arcteonais lomondi 9 Dem app. 17 Dero digitata 95 9 3-11 Table 3-4. (Continued). 2010 Locations Taxa A B F E Oligochaeta Dery bifida 155 439 Nais spp. 9 Nais partialis 9 llncinais uncinata ___ 9 Tubth1 cidae .903 121 250 207_ Aulockilus limnobius 801 34 232 52 Aulodrilus pigueti 138 844 Branchirua sowerbyi 232 34 43 172 Illyvdrilus templetoni 60 Limnodrilus hoffineisterei 26 9 Tubifer tubifex 43 Other F linea Glossiphoniidae Helobdella stagnalis 336 26 34 SabeTidae Manayunkia speciosa 26 336 Nematoda 164 77 Pelecypoda Corbicubdae Corbicula flumina 551 1,369 34 Trichoptera Leptoceridae Oecetis spp. 9 9 Triaenodes spp. 17 Polycentropodidae Aalycentrvpus spp. 17 Total Density for Year 6,338 3,125 5,468 1,130 Total Taxa for Year 29 28 31 21 3-12 Table 3-5. Macroinvertebrate taxa and densities (No./m2) at each Lake Norman sampling location during summer sampling in 2011. 2011 Locations Taxa A B F Lijara Ceratopogonidae Palpomyia-Bezzia complex 26 9 26 34 Chironomidae-Chironominae Ghironomus spp. 9 Cladopelma spp. 9 26 C2adotanytarsus spp. 164 17 319 26 Cryptochirtnnus spp. 9 69 26 Cryptotendpes spp. 9 551 121. 26 Dic otendpes spp. 9 95 Nilothauma spp. 9 Pagastiella spp. 9 26 Paralauterbomiella niujohalteralis 26 PclyperWum halterale gr. 17 164 34 9 Pblypedlum scalaenum gr 43 Stempellina spp. 9 9 26 Stictochironomus spp. 146 Sticto iironomus spp. 9 646 Stictochiranomus caffranius 103 Tanytarsus spp. 112 77 43 17 Chiron Paraloefieriella spp. 17 Chironomidae-Tanypodnae Ablabesmyia annulata 34 17 Ablabesmyia mallochi 9 Ablabesmyia ramphe gr. 9 9 Coelotanypus spp. 405 232 26 284 Prodadius spp. 250 284 77 Ephemeroptera Baetidae Pseudodoean spp. 9 276 387 Caenidae Caenis spp. 34 Ephemeridae Ibxagenia app. 34 43 43 Megaloptera Sialidae Sialis app. 9 Oligochaeta Naiddae 26 17 Arcteonais lomandi 9 Dero spp. 9 Dero digitata 267 Dero bifida 26 474 3-13 Table 3-5. (Continued). 2011 Locatins Taxa A B F E Oligochaeta Tubihcidae 835 112 740 129 Aulodnlus limnobius 52 60 86 Autodrilus piquet' 491 Limnodrilus horfineisterei 9 9 Other Glossiphonidae I welobdella stagnalis Ti 77 370 17 Planariidae Cora fonnanii 9 34 Nematoda 95 129 293 9 Pelecypoda CorbicuGdae Corbicula flumina 138 422 465 Trichoptera Hopes Hydroptila spp. 17 Leptoceridae Oecetis spp. 43 17 26 Polycentropoddae Polycentropus spp. 9 Total Density for Year 2,517 3,119 4,238 1,326 Total Taxa for Year 24 30 24 18 3-14 Table 3-6. Macroinvertebrate taxa and densities (No./m2) at each Lake Norman sampling location in during summer sampling in 2012. 2012 Locations Taxa A B F E Diptera Ceratopogonidae Palpomyia-Bezzia complex 9 60 Chaoboridae Chaoborus spp. 26 Chironorridae-Chirawminae Chironomus spp. 9 17 Ciadopelma spp. 9 C adotanytarsus spp. 103 577 9 Cryptochiranornus spp. 43 164 52 Cryptotendipes spp. 17 9 215 103 Dicrotendipes neomodestus 34 Pagastiella spp. 9 9 17 Paralauterbomiella nigrohalteiralis 26 Polypedilum halter-ale gr. 121 26 Polypedilum scalaenum gr. 69 52 Stempellina spp. 17 Stictochironomus spp. 379 189 172 Tanytarsus spp. 17 293 52 Ch'uono Paraldefferiella spp. 9 Chironomidae-Tanypodinae Ablabesmyia annulata 9 9 Ablabesmyia mallochi 9 34 Coelotanypus spp. 112 9 138 Djalmabatista pukka 17 Procladius spp. 577 26 103 Ephemeroptera Baetidae Pseudoclbecn spp. 26 Caenidae Caenis spp. 77 Ephemeridae Hexagenia spp. 9 9 181 Megaloptera Sialidae Sialis spp. 34 Oligochaeta Naididae 86 Arcteonais lomondi 9 250 69 Bratislavia unidentata Dem spp. 34 9 Dego digitata 1,825 3-15 Table 3-6. (Continued). 2012 Locations Taxa A B F E Oiigochaeta Ndae Dero bifida 95 121 17 Nais spp_ 43 Nais partialis 17 PristineJlajenkinae 52 Uncinais uncinata 456 990 Tubifcidae 964 611 1,558 26 Aulodn7us limnobius 34 43 Aulodrilus pigueti 9 1,171 Aulodrilus pluriseta 9 43 Branchirua sowerbyi 86 482 207 26 Other Glossiphoniidae flelobdella stagnalis 17 26 Glossiphonndae Heiobdelia stagnalis 241 Planarndae Cura formanii 34 Tetrastemmatidae Prostoma graecaens 9 Nematoda 26 52 293 26 Pelecypoda CorbicuGdae Corbicula flumina 69 52 86 34 Sphaeriidae 17 121 Trichoptera Leptoceridae Oecetis spp_ 34 17 Polycentropadidae Polycentropus spp. 9 9 Total Density for Year 2,634 5,116 5,703 1,353 Total Taxa for Year 21 20 30 27 3-16 Table 3-7. Macroinvertebrate taxa and densities (No./m2) at each sampling location in Lake Norman during summer sampling in 2013. 2013 Locations Taxa A B F E Diptera Ceratopogonidae Palpcmyia-Bezzia complex 9 164 52 17 Chaoboridae Chaobonrs app. 9 9 Chironornidae-Cturonominae Chiranomus app. 26 86 Cladopelma spp. 9 17 Cladotanytarsus spp. 689 207 69 Cryptochironomus spp. 52 34 43 26 Cryptotendrpes spp. 103 250 86 34 Hamischia spp. 17 Abcrochironomus spp. 9 Nilothauma spp. 9 Pagastiella spp. 9 52 17 Paralauterbomiella nigrohalteralis 112 17 95 9 Polypedilum halterale gr. 129 198 52 Polypedilum scalaenum gr. 43 Stempellina spp_ 9 17 9 9 Stictochironomus spp. 697 534 86 Tanytarsus spp. 362 215 77 224 Chiron ' Nanodadius spp. 9 Chironomidae-Tanypodinae Ablabesmyia annulata 34 43 17 Ablabesmyia mallochi 26 Coelotanypus spp. 284 112 52 86 Djalmabatista puk rra 9 Prodadius spp. 396 525 52 267 Ephemeroptera Baetidae Pseudocloeon spp. 26 17 138 9 Caenidae Caenis spp. 26 Ephemeridae Hexagenia spp. 34 17 9 43 Megaloptera Sialidae Sialis spp. 17 9 011gochaeta Naididae 86 Arcteonais lomondi 103 9 95 Dero spp. 17 Dem digitata 52 17 Dem bifida 69 224 17 Uncinais uncinata 69 413 34 3-17 Table 3-7. (Continued). 2013 Locations Taxa A B F E Oligochaeta Tubiicidae 1,576 947 1,395 603 Aulodrilus limnabius 77 319 542 Aulodrilus pigueti 34 3,384 Aulodrilus plurisefa 34 Branchirua sowerbyi 387 1,128 620 Branchirua sowerbyi 60 illyodrilus templetoni 129 69 Limnodrilus hoffineisterei 34 Other Glossiphoniidae Helobdella sfagnalis 232 1,240 465 52 Placabdella spp. 9 Planariidae Curs formanii 17 Sabetidae Manayunlda speciosa 26 43 Talitridae Hyalella azteca 43 Nematoda 284 456 482 121 Pelecypoda Corbiculidae Corbicula flumina 112 9 758 26 Sphaeriidae Sphaerium spp. 43 26 353 Trichoptera Hydroptilidae Hydroptila sPp- 9 Leptoceridae Oecetis spp. 69 34 95 17 Total Density for Year 6,318 9,300 5,515 3,686 Total Taxa for Year 36 27 29 34 3-18 ❑A ❑ B ❑ F ❑ E 45 — 40 = I 35 U CJ 25ca x 20 — ^— Ta ►- 15 10 5 0 f ' 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 Figure 3-1. Total number of taxa collected annually from Lake Norman in the vicinity of MSS, 2004— 2008 and 2009— 2013. OA ❑ B ❑ F ❑ E 10,000 T 9,000 8,000 7,000 E 6,000 — 5,000 - — — 4,000 — 3,000 - -- 2,000 ' - 1,000 / p , 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 Years Figure 3-2. Densities (No./m2) of macroinvertebrates collected annually from Lake Norman in the vicinity of MSS, 2004—2008 and 2009 — 2013. 3-19 OA EIB OF OE if Oligochaeta 7,000 at 6,000 .4 .-4 5,000 o 4,000 - - 1- 3 000 , 0 2,000 • • 1,000 • — I — 0 + - • _ 2004 2005 2006 2007 2008 2009 2010 2011 20:2 2013 Years Figure 3-3. Densities (No./m2) of Oligochaeta collected annually from Lake Norman in the vicinity of MSS, 2004—2008 and 2009—2013. DA DB OF OE Chironomidae 3,000 2,500 N E 2,000 — 1,500 c 1,o00 _ – w – SOO _ — 2004 2005 2005 2007 2008 2009 2010 2011 2012 2013 Years Figure 3-4. Densities (No./m2) of Chironomidae collected annually from Lake Norman in the vicinity of MSS, 2004—2008 and 2009—2013. 3-20 DA BB OF OE Non-Chironomids 300 250 II h E 200 i o I -- 150 i . .. .. _._ Zvi c 100 a _ S0 '«; 0 .f ri0 ,� 0i®. r .11 w I j11° L , 0 ° + r 1i 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 Years Figure 3-5. Densities (No./m2) of non-chironomid taxa from Lake Norman in the vicinity MSS, 2004—2008 and 2009—2013. DA OB OF DE Corbicula 2,500 - N N I o 2 000 _ .J (» z 1,soo II, Z .N 1,000 c i r — C.) — 500- 'a x 0 r'l + Ork al le 1 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 Years Figure 3-6. Densities (No./m2) of Corbicula collected annually from Lake Norman in the vicinity of MSS, 2004—2008 and 2009—2013. 3-21 NIA ® B OF DE Ephemeroptera 450 400 _ 350 - N E 300 )11 -. Z 250 -. Z' 200 150 -. — — — 0 '- — SO -1 • 0 0 tO 2004 2005 2006 2007 2006 2009 2010 2011 2012 2013 Years Figure 3-7. Densities (No./m2) of Ephemeroptera collected annually from Lake Norman in the vicinity of MSS, 2004—2008 and 2009—2013. BA BB OF DE Trichoptera 120 100 E 8o _. Z 60 g C 40 — 0 — 20 _ C CC 0 2004 2005 2006 2007 2006 2009 2010 2011 2012 2013 Years Figure 3-8. Densities (No./m2) of Trichoptera collected annually from Lake Norman in the vicinity of MSS, 2004—2008 and 2009—2013. 3-22 OA OB BF DE Megaloptera 45 40 35 30 z25 Z" 20 1/1 0 15 -. 0 10 - 5 0 ;.0 OHOI 0 00 000! 000 000 , 0 0 ' 00 000 101 0 20C4 2005 2006 2007 2008 2009 2010 2011 2012 2013 Years Figure 3-9. Densities (No./m2) of Megaloptera from Lake Norman in the vicinity of MSS, 2004—2008 and 2009—2013. OA MB OF OE Spearidae 500 m 400 6 300 - Z 200 - 100 0 00 0000 0000 00] 0 -1=1 • I1J LI L 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 Years Figure 3-10. Annual densities (No./m2) of Spheariidae from Lake Norman in the vicinity of MSS, 2004—2008 and 2009—2013. 3-23 DA ❑ B OF El 500 400 E 300 o z •w c 0 200 100 — — 0 _-1-, _ _ n [ _ — 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 Years Figure 3-11. Densities (No./m2) of EPT from Lake Norman in the vicinity of MSS, 2004 — 2008 and 2009—2013. DA OB OF DE Excellent 30 Good 25 20 — 2 Good tan 15 — x cv W 1p -Fair — 0 ' -1 — _ 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 Years Figure 3-12. EPT taxa richness from Lake Norman in the vicinity of MSS, 2004 — 2008 and 2009—2013. 3-24 CHAPTER 4 FISH MATERIALS AND METHODS Spring Electrofishing Surveys Annual (2009 — 2013; current National Pollution Discharge Elimination System permit cycle)boat electrofishing surveys were continued in Lake Norman in March or April near the thermal influence of Marshall Steam Station (MSS; Zone 4 in Figure 4-1) and at a reference area (REF, Zone 3) located between MSS and McGuire Nuclear Station. Ten 300-m shoreline transects were surveyed in each area and were identical to historical locations surveyed since 1993. Transects included littoral habitats representative of those found in Lake Norman. All sampling was conducted when surface water temperatures were expected to be 15—20 °C. Stunned fish were collected by two netters and identified to species. Fish were enumerated and weighed in aggregate by taxon, except for spotted bass (Micropterus punctulatus) and largemouth bass (M. salmoides), where total length (TL, mm) and weight (g) were obtained for each individual collected. Surface water temperature(°C)was measured with a calibrated thermistor at each transect. Annual catch per unit effort (CPUE per 100 m) was determined by area for number of individuals,biomass, and number of species collected. Condition(Wr) based on relative weight was calculated for spotted bass and largemouth bass > 150 mm TL, using the formula Wr = (W/Ws) x 100, where W = weight of the individual fish and Ws = length-specific mean weight for a fish as predicted by a weight-length equation for each species (Neumann et al. 2012). Resulting metrics were compared between areas using a t- test(P < 0.05). Summer Electrofishing Surveys Annual (2009 — 2013) boat electrofishing surveys were continued in Lake Norman in July near the thermal influence of MSS. Ten 100-m shoreline transects were surveyed to represent three areas (Figure 1-1): two transects above the MSS discharge canal (Location 4-1 A), four in the vicinity of the discharge canal (Locations B and C), and four below the discharge canal (Locations D and E). Transects were identical to historical locations surveyed since 1991 and independent of spring transects. Surface water temperature and dissolved oxygen (DO, mg/L) were measured with a calibrated thermistor and DO probe, respectively, at each location. Stunned fish were collected by two netters, identified to species, and measured for TL. Representative and Important Species (RIS) were selected based upon abundance, distribution, and ecological significance as a prey (bluegill [Lepomis macrochirus] and redbreast sunfish [L. auritus])or predator(spotted bass) species. Fall Hydroacoustic and Purse Seine Surveys Density and distribution of pelagic forage fish in Lake Norman were determined using mobile hydroacoustic (Rudstam et al. 2012) and purse seine (Hayes et al. 2012) techniques. The lake was divided into zones (Figure 4-1) due to its large size and habitat spatial heterogeneity. An annual mobile hydroacoustic survey was conducted in mid-September with multiplexing, side- and down-looking transducers to detect surface-oriented fish and deeper fish(from 2.0-m depth to the bottom), respectively. Annual purse seine samples were also collected in mid-September from the epilimnion of downlake (Zone 1), midlake (Zone 2), and uplake (Zone 5) areas in water deep enough for unhindered net deployment. The purse seine measured 122.0 x 9.1 m, with a mesh size of 4.8 mm. A subsample of forage fish collected from each area was used to estimate taxa composition and TL size distribution. Balanced and Indigenous Assessment Annual surveys are used to assess the balanced and indigenous nature of the Lake Norman fish community and provide information relative to the potential thermal influence of MSS. The assessment includes comparisons of spring electrofishing CPUE (spatially and temporally) and of summer electrofishing RIS length distributions. Results from both seasonal surveys were examined according to species pollution tolerance and trophic guild. Hydroacoustic and purse seine surveys of pelagic forage fish were examined for trends. 4-2 RESULTS AND DISCUSSION Spring Electrofishing Surveys Spring electrofishing surveys from 2009 to 2013 were conducted at average water temperatures ranging from 15.9 to 21.1 °C. Surveys resulted in the collection of 11,682 individuals comprising 26 species at the MSS area and 10,251 individuals comprising 21 species at the REF area (Table 4-1). The number of individuals per 100 m ranged from 47.3 to 143.3 at the MSS area and from 59.2 to 77.2 at the REF area (Table 4-1 and Figure 4-2). Fish biomass per 100 m ranged from 3.5 to 7.1 kg at the MSS area and from 2.0 to 3.9 kg at the REF area (Figure 4-3). The number of species per 100 m ranged from 14 to 20 at the MSS area and from 14 to 19 at the REF area (Figure 4-4). Since 1993, both biomass and number of species collected from the MSS area were significantly greater than those from the REF area. When limited to the current permit cycle, the biomass collected from the MSS area was significantly greater than from the REF area. The number of individuals in spring electrofishing surveys from 2009 to 2013 was dominated by centrarchids (MSS-95.9%, REF-93.7%), with clupeids (MSS-1.5%, REF-3.3%) and cyprinids (MSS-1.4%, REF-2.3%) greater than 1.0% each, and the remaining families representing less than 1.0% of individuals combined (Table 4-1). Green sunfish (Lepomis cyanellus) exhibited a considerable (and similar) increase in relative abundance at both areas compared to the 2004 - 2008 study period. Overall, current species composition data are similar to previously reported spring electrofishing data near MSS (Duke Power Company 1994; Duke Power 1999, 2004a; Duke Energy 2009a) and from other Catawba River reservoirs (Duke Power 2004b, 2004c; Duke Energy 2009b, 2009c). Species considered pollution tolerant for wadeable stream assessments (e.g., longnose gar [Lepisosteus osseus], goldfish [Carassius auratus], common carp [Cyprinus carpio], golden shiner [Notemigonus crysoleucas], white catfish [Ameiurus catus], redbreast sunfish, green sunfish, and hybrid sunfish; NCDENR 2013) represented 19.7% of MSS and 27.5% of REF individuals during spring 2009 - 2013, similar to spring 2004 - 2008 (MSS-18.1%, REF- 24.2%) and spring 2000-2003 (MSS-15.4%, REF-17.0%). Species considered insectivorous for wadeable stream assessments (NCDENR 2013) represented 86.0% of MSS and 86.3% of REF from 2009 to 2013. Species considered 4-3 piscivorous (MSS-11.2%, REF-8.8%) and omnivorous (MSS-2.8%, REF-4.9%) also reflected similar guild contributions when comparing areas from 2009 to 2013. No significant difference existed between areas for spotted bass mean Wr (MSS-77.2, REF- 77.1). Largemouth bass from MSS (84.5) had a higher mean Wr than from REF (82.8); however, the continued downward trend in the number of largemouth bass collected from Lake Norman in recent years diminishes the significance of a statistical comparison. Summer Electrofishing Surveys Summer electrofishing surveys from 2009 to 2013 resulted in the collection of 2,967 individuals comprising six families, 19 species, and two hybrid centrarchid combinations (Table 4-2). The species composition for the combined summer electrofishing surveys was dominated by centrarchids (93.2%), followed by clupeids (4.9%), and cyprinids (1.2%). The remaining families represented less than 1.0% of individuals combined. Previously reported summer data also documented dominance by centrarchids during 2004 — 2008 (90.4%) and 2000 — 2003 (77.0%). Spotted bass have steadily increased in percent composition from being absent prior to 2005 to approximately 9.5% of individuals collected during summer 2009 —2013. Overall, current CPUE and species composition data are similar to previously reported summer electrofishing data near MSS (Duke Power Company 1994; Duke Power 1999, 2004a; Duke Energy 2009a) and from other Catawba River reservoirs (Duke Power 2001a, 2001b, 2004b, 2004c; Duke Energy 2009b, 2009c). Pollution-tolerant species represented 16.6% of the collected fish during summer 2009 — 2013 surveys, similar to summer 2004 — 2008 (12.2%) and summer 2000 — 2003 (18.2%). Insectivorous (78.2%), piscivorous (15.8%), and omnivorous (6.0%) species from 2009 to 2013 surveys had guild contributions similar to summer 2004 — 2008 (79.4%, 12.3%, 8.3%) and summer 2000—2003 (81.6%, 12.9%, 5.6%),respectively. The TL distributions of RIS (i.e., bluegill, redbreast sunfish, and spotted bass) during summer 2009 — 2013 surveys (Figures 4-5 to 4-7) indicated successful reproduction and multiple age groups from above, in the vicinity of, and below the MSS discharge canal. Although the discharge canal consistently had the highest temperature (as high as 36.8 °C) and lowest DO (as low as 0.5 mg/L), it maintained RIS TL size class distributions similar to areas above and below the discharge canal. 4-4 Fall Hydroacoustic and Purse Seine Surveys Annual hydroacoustic estimates near MSS (Zone 4) from 2009 to 2013 indicated the regular availability of pelagic forage fish with no temporal trend in density (range = 1,564 — 11,551 fish/ha; Figure 4-8). Threadfin shad (Dorosoma petenense) continued to dominate annual purse seine surveys of the Lake Norman forage fish community from 2009 to 2013, comprising 88.4 — 98.3% of fish collected (Table 4-3). Alewife (Alosa pseudoharengus), first detected in low numbers in 1999 (Duke Power 2000), have comprised as much as 25.0% (2002) of mid-September pelagic forage fish surveys, but percent contribution has remained relatively low since 2005 (range= 1.5— 11.6%). CONCLUSIONS A diverse fish community was present in the littoral surveys of Lake Norman near MSS from 2009 to 2013. Spring and summer electrofishing documented 28 and 19 species, respectively, both numerically dominated by centrarchids, especially bluegill. Pollution- tolerant species comprised less of the spring MSS fish population (19.7%) relative to the REF area (27.5%), and comprised only 16.6% of individuals collected during summer surveys. The assorted fish species typically found in Lake Norman near MSS encompass multiple trophic guilds (i.e., insectivores, omnivores, and piscivores) supporting a balanced fish community. Non-indigenous species such as spotted bass and green sunfish are abundant near MSS,but are also prevalent throughout Lake Norman. Annual hydroacoustic estimates from 2009 to 2013 showed the regular availability of pelagic forage fish near MSS. Purse seine surveys indicate that threadfin shad continue to dominate the Lake Norman forage fish community with a consistent alewife composition of approximately 5% after 2004. The introduction of alewife and inherent, temporal fluctuations in clupeid densities contribute to the variable nature of forage fish populations. Past studies have indicated that a balanced indigenous fish community exists near MSS (Duke Power Company 1994; Duke Power 1999, 2004a; Duke Energy 2009a). The present study adds more years of comparable data, reinforcing that conclusion. Based on the CPUE of littoral fish during spring, TL distributions of RIS during summer, and the regular availability of forage fish, it is concluded that the thermal discharge of MSS has not impaired the Lake Norman fish community. 4-5 Table 4-1. Total number of individuals,percent composition, and total number of species in spring electrofishing surveys from two areas (MSS and REF)in Lake Norman, 1993 - 1997 and 1999, 2000-2003,2004-2008, and 2009-2013. Spmg 1993-1997,1999 Sprig 2000-2003 Sprig 2004-2008 Sprig 2009-2013 PASS REF PASS REF MSS REF PASS REF Sclen9Fk:nano Comrcn name No. % No. % Pb. % Pb. % Pb. % -Pb. % _ Pb. % Pb. % Lepisostsldas Lepiaoaeea oeseua Lo gnoseger 2 0.03% 1 0.01% 1 0.01% 1 0.01% 5 0.05% Clupildas Alosspseudo5arengua Alewife 45 0.51% 57 0.77% 1 0.01% 51 0.53% 2 0.02% 1 0.01% Don ecma cepedanum Gizzard shed 81 0.94% 50 0.72% 30 0.34% 94 1.27% 27 0.32% 38 0.39% 84 0.72% 60 0.59% Darosanapitenenae TMeedfinshad 944 10.98% 1,515 21.72% 840 9.46% 58 0.78% 127 1.51% 523 5.43% 86 0.74% 276 2.69% Cyprinidae Cyprineda ch/aieda Greenfin shier 22 0.26% 12 0.17% 50 0.56% 153 2.07% 43 0.51% 38 0.39% 16 0.15% 49 0.48% Cy4nelladnee WnhfInshimer 734 &54% 586 8.40% 1257 14.18% 1,470 19.89% 228 2.72% 353 3.67% 28 0.22% 44 0.43% Cyprinua caplo Connor cap 157 1.83% 168 2.41% 190 2.14% 51 0.69% 58 0.69% 48 0.50% 99 0.85% 20 0.20% H18ogne(Mis regius Eastern savary ninnow 2 0.02% Nocaas lsptocephdus Behead chub 1 0.01% NotaTgars s ayadsucas Golden shies 8 0.09% 5 0.07% 4 0.06% 2 0.03% 7 0.08% 1 0.01% 1 0.01% Nobrophehudsonlus Spottal shiner 414 4.81% 67 0.98% 472 5.32% 561 7.59% 240 2.86% 184 1.91% 21 0.18% 119 1.16% Notropisprocne Swallowtail shiner 1 0.01% 1 0.01% Pfinephales prame/as Fathead minnow 1 0.01% Catostom Mae Cwpodes cgxinua Oulback 5 0.06% 6 0.09% 3 0.03% 3 0.04% 2 0.02% 4 0.04% 3 0.03% M orostoma mapdepidofum ShorOwd redhase 2 0.02% 4 0.06% 4 0.05% 1 0.01% Moxostune npiscartes Striped}arprock id. 1 0.01% Ictalur Amedu u8 calm MAW catfish 1 0.01% Amalwus nebWosua Brown bullhead / 0.01% Iclalurus*rcalua Beep catfish 2 0.02% 8 0.11% 1 0.01% 3 0.03% 1 0.01% Icialuruspunctau8 Channel catfish 22 0.26% 14 0.20% 37 0.42% 40 0.54% 16 0.19% 24 025% 35 0.30% 27 0.26% Pyfcisesdiwris Hothead catfish 7 0.08% 4 0.06% 14 0.16% 6 0.06% 9 0.11% 14 0.15% 21 0.18% 40 0.39% Salmonidae Oncorhynchua myklsa Rainbow trout 2 0.02% 1 0.01% Moronklaa Moron americana Wide perch 8 0.09% 103 1.16% 4 0.05% 8 0.10% 17 0.18% 66 0.56% Morse cirysops Mlle bass 6 0.07% 4 0.06% 2 0.02% 3 0.04% Morme saxadlla Striped bass 6 0.07% 3 0.03% 2 0.03% 1 0.01% 1 0.01% 10 0.09% 3 0.03% Centrarchldae Lspomisavdua Redbreast sunfish 1,398 1626% 1,017 14.58% 957 10.78% 1,004 13.59% 1,110 13.24% 1,925 19.99% 587 5.02% 1,408 13.74% Upends cyarellua Green sunfish 129 1.54% 12 0.12% 1,373 11.75% 1,084 10.57% Lepania gibbosue Rarpkeeeed 4 0.05% Wanes guloaus Wamou h 63 0.73% 95 1.36% 150 1.69% 118 1.60% 111 1.32% 225 2.34% 69 0.59% 125 1.22% Lepamis hybrd Hybdd sunfish 219 2.56% 207 2.97% 213 2.40% 197 2.67% 340 4.06% 360 3.74% 245 2.10% 306 2.99% Worlds mecroderus Bkregi 2,731 31.76% 1.939 27.80% 3.418 38.51% 2,622 36.48% 4,600 54.85% 4,735 49.16% 7,424 63.55% 5,634 54.96% Lepomis miaoicphus Pledger sunfish 111 1.29% 174 2.49% 468 5.27% 477 6.45% 559 6.67% 558 5.79% 300 2.57% 199 1.94% Mrcrcptaus punctdalus Spaded bass 15 0.17% 12 0.16% 304 3.63% 198 2.06% 929 7.95% 750 7.32% MOcropterus salmoides Largemouth bass 1,349 15.69% 911 13.06% 573 6.46% 420 5.68% 408 4.87% 270 2.80% 221 1.89% 73 0.71% M9cropterus hybrid HMO beck bass 19 0.23% 11 0.11% 43 0.37% 18 0.18% Ponexis ammlais Meds crappie 3 0.03% Pbmaris dgramacdaus Beck crappie 51 0.59% 118 1.69% 1 0.01% 19 0.26% 19 0.23% 22 023% 12 0.10% 8 0.06% Partial.. a3heoebrre Maihxme Swamp darter 1 0.01% Maxims dmsted Tessellated dater 1 0.01% 1 0.01% 1 0.01% 3 0.04% 3 0.04% 4 0.04% 1 0.01% Percy Bawacene Yellow perch 256 2.98% 80 1.15% 17 0.19% 3 0.04% 8 0.10% 13 0.13% 1 0.01% Total 8,599 100.00% 6,976 100.00% 6,876 100.00% 7,390 100.00% 8,388 100.00% 9,631 100.00% 11,682 100.00% 10,251 100.00% Total no.speedos 24 21 26 27 26 25 26 21 LT Table 4-2. Total number of individuals,percent composition, and total number of species in summer electrofishing surveys from three areas near MSS (above,in the vicinity of, and below the discharge canal)in Lake Norman, 1991 - 1993, 1994- 1999, 2000-2003, 2004-2008, and 2009-2013. Sumner 1991-1993• Sumner 1994-1999° Sumner 2000-2003 Sumner 2004-2008 Sumner 2009-2013 Scientific name Corroon name Pb. % No. % No. % Pb. % Pb. % Leplsosteidas Lepisosteus osseus Longnose gar 11 0.26% 3 0.09% 3 0.10% Clupeldae Alosa pseudoharengus Alewife 5 0.16% 2 0.07% Darosoma cepedlennum Gizzard shad 11 0.72% 38 0.91% 4 0.19% 6 0.19% 12 0.40% Darosome petenense Threadfin shad 5 0.33% 355 8.54% 60 1.88% 130 4.38% Cyprinidae Carassius auratus Goldfish 1 0.03% 1 0.03% Cyprinella chlorlstie Greenfin shiner 15 0.98% 54 1.30% 28 1.32% 53 1.66% 7 0.24% Cyprinella nivee Whitefin shiner 170 11.10% 469 11.28% 257 12.09% 58 1.82% 1 0.03% Cyprinus cerplo Conran carp 53 3.46% 94 2.26% 11 0.52% 3 0.09% 7 0.24% Notemigonus crysoleucas Golden shiner 5 0.12% Notropis hudsonius Spottal shiner 29 0.70% 157 7.39% 100 3.14% 20 0.67% Catostomides Carpiodes cyprinus Callback 5 0.12% Monostoma macrdepidolum Shorlhead red arse 7 0.33% 5 0.16% Moxostoma sp. Brassyjunprock 6 0.14% Ictaluridae Ictalurus punctatus Channel catfish 11 0.72% 10 0.24% 4 0.19% 7 0.22% 8 0.27% Pylodctis olivaris Flathead catfish 16 0.38% 2 0.09% 3 0.09% 7 0.24% PoecIlldae Gambusia holbrooki Eastern rrosqutofish 5 0.33% 3 0.10% lloronidae Marone americana White perch 8 0.38% Moron.chrysops White bass 5 0.12% Centre/chides Lepornis aurilus f2adereast sunfish 119 7.77% 261 6.28% 317 14.92% 272 8.53% 155 5.22% Lepomis cyanellus Green sunfish 5 0.33% 1 0.05% 58 1.82% 286 9.64% Leponds gibbosus f'unpkinseed 5 0.33% Lepomis gulosus Warnouth 26 1.70% 91 2.19% 42 1.98% 74 2.32% 88 2.97% Lepomis hybrid Hybrid sunfish 5 0.33% 99 2.38% 58 2.73% 53 1.66% 37 1.25% Lepomis macrochirus Bkregll 926 60.44% 1,829 43.98% 900 42.35% 1,957 61.41% 1,703 57.40% Lepamis microlophus Redear sunfish 5 0.33% 71 1.71% 78 3.67% 65 2.04% 38 1.28% Micropterus punctulatus Spotted bass 86 2.70% 282 9.50% Micropterus salmoides Largennulh bass 133 8.68% 422 10.15% 240 11.29% 313 9.82% 170 5.73% Micropterus hybrid Hybrid black bass 2 0.06% 7 024% Pomoxlsnigromeculatus Black crappie 6 0.14% 1 0.05% Percldas Etheostoma olmstedi Tessellated darter 20 1.31% 26 0.83% Perca Bavescens Yelow perch 18 1.17% 257 6.18% 10 0.47% 3 0.09% Total 1,532 100.00% 4,159 100.00% 2,125 100.00% 3,187 100.00% 2,967 100.00% Total no.species 16 21 17 20 19 A •locations B and E w ere not sarrpled in 1992.location B was not sanpied in 1993 J °location B w as not sampled in 1994,locations A and B were not sampled in 1995 Table 4-3. Pelagic forage fish species composition from purse seine surveys in Lake Norman, 1993 -2013. Species composition Year Threadfin shad Gizzard shad Alewife 1993 100.00% 1994 99.94% 0.06% 1995 99.95% 0.05% 1996 100.00% 1997 99.99% 0.01% 1998 99.95% 0.05% 1999 99.26% 0.26% 0.48% 2000 87.40% 0.22% 12.37% 2001 76.47% 0.01% 23.52% 2002• 74.96% 25.04% 2003 82.59% 0.14% 17.27% 2004 86.55% 0.24% 13.20% 2005 98.10% 1.90% 2006 94.87% 5.13% 2007 98.34% 1.66% 2008 95.58% 4.42% 2009 88.40% 11.60% 2010 95.38% 0.36% 4.26% 2011 98.32% 0.15% 1.52% 2012 93.60% 6.40% 2013 93.50% 6.50% 4-8 Zone 6 • Purse seine locations • Electrofishing transects Zone 5 � � 9 ; Marshall Steam Station �" N. L • • Zone 4 r7 • gam```1 414: h h Zone 2 • • Zone 3 • $Y' Lt, fe _± •, , 0 0.5 1 2 3 �' • Miles • e Zone 1 0 1 2 4 — Cowans Ford Dame • Kilometers McGuire Nuclear Station - Figure 4-1. Locations associated with spring electrofishing and fall purse seine surveys, and zones associated with fall hydroacoustic survey of Lake Norman. 4-9 160 — -MSS 140 — f REF 120 — - 100 — 80 — 0 r 60 — 40 — 20 — 0 1 + + + + + 1 1 + + + 1 + + 1 1 + + + 1 i co La Co f� co a) o N M 1.0 O CO O O N M O O O O O O O O O O O O O O O O O O O 6) O O 67 O O O O O O O O O O O O O O O N N N N N N N N N N N N N N Year Figure 4-2. Total number of fish collected in spring electrofishing surveys from two areas (MSS and REF) in Lake Norman, 1993 — 1997 and 1999—2013. 14 — MSS 12 — —f—REF 10 — E 0 8 — 0 m 6 — N LL 4 — r . • 2 — co '• O co on o N M Li CD f� CO O O N O 0) 0) O O O O O O O O O O O O O o O O Cr) 0) O O O O O O O O O O O O O O O NNNNNNNNNNNNNN Year Figure 4-3. Biomass of fish collected in spring electrofishing surveys from two areas (MSS and REF) in Lake Norman, 1993 — 1997 and 1999 —2013. 4-10 30 - +MSS 25 - -t-REF 20 - 0 el\ ViVq6%j- 15 - o) 0 'U N s 10 — ii 5 - 0 I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 M v' it) (O Co O o N M O ti CO O O '- N CO Cn C7) O) O Cn O 0) O O O O O O O O O O .- O O O O CS) O CS) O O O O O O O O O O o O O o N N N N N N N N N N N N N Year Figure 4-4. Total number of fish species collected in spring electrofishing surveys from two areas (MSS and REF) in Lake Norman, 1993 — 1997 and 1999—2013. 16% - ■Above ■Discharge ■Below 14% - 12% -y 10% — 0 0 8% — c v 6% a� o- 4% ill" 2%0% -I' I L .Y I1Iki _L all I_ 1 _ O o o O o 0 0 0 0 0 N CD Co O N CO Co r r r r r N IL class(mm) Figure 4-5. Length distribution of bluegill among survey locations in summer electrofishing surveys near MSS in Lake Norman, 2009—2013. 4-11 25% — ■Above ■Discharge ■Below 20% c 0 15% - - 0. E 0 0 t00i 10% - - a 5% — tII 11111111111111 11111 I 11111 11 NO COO coo O N COO co O N TL class(mm) Figure 4-6. Length distribution of redbreast sunfish among survey locations in summer electrofishing surveys near MSS in Lake Norman, 2009—2013. 30% — ■Above ■Discharge ■Below 25% — 0 20% — U, 0 o 15% c I a10% — ::: : - +� lir I I III I'I 1111111 It, li ; , I I11 O O O O O O O o O O O O O O N CO CO O N CO CO N N N N CO M TL class(mm) Figure 4-7. Length distribution of spotted bass among survey locations in summer electrofishing surveys near MSS in Lake Norman, 2009—2013. 4-12 25,000 — —a—Zone 1 —A—Zone 2 —A—Zone 3 Zone 4 Zone 5 20,000 — cT s °c 15,000 C 7 a) r 10,000 — w a) f2 —van. N4414 O) ` v 5,000 — `0f»�''\ /'/ 0 I I I I I I E t I I I I I I I I I N. co 0) O — N Cr) U) CO f- co C) O N M C) C) C) 0 0 0 0 0 0 0 0 0 0 A- A- A- e- C) C) C) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 a- a- a- N N N N N N (N N N N N N N N Year Figure 4-8. Pelagic forage fish density estimates by zone in Lake Norman, late summer/early fall 1997—2013. 4-13 LITERATURE CITED Brinkhurst, RO. 1974. The Benthos of Lakes. The MacMillan Press, London. 190 pp. Duke Energy. 2008. Lake Norman maintenance monitoring program: 2007 Summary. Duke Energy. 2009a. Assessment of balanced and indigenous populations in Lake Norman near Marshall Steam Station. Duke Energy, Charlotte,NC. Duke Energy. 2009b. Assessment of balanced and indigenous populations in Lake Wylie near Allen Steam Station. Duke Energy, Charlotte,NC. Duke Energy. 2009c. Assessment of balanced and indigenous populations in Mountain Island Lake near Riverbend Steam Station. Duke Energy, Charlotte,NC. Duke Energy. 2009d. Lake Norman maintenance monitoring program: 2008 Summary. Duke Energy, Charlotte,NC. Duke Energy. 2011. Lake Norman maintenance monitoring program: 2010 Summary. Duke Energy, Charlotte,NC. Duke Energy. 2012. Lake Norman maintenance monitoring program: 2011 Summary. Duke Energy, Charlotte,NC. Duke Energy. 2013. Lake Norman maintenance monitoring program: 2012 Summary. Duke Energy, Charlotte, NC. Duke Power. 1999. Assessment of balanced and indigenous populations in Lake Norman near Marshall Steam Station. Duke Power, Charlotte,NC. Duke Power. 2000. Lake Norman maintenance monitoring program: 1999 summary. Duke Power, Charlotte,NC. Duke Power. 2001 a. Assessment of balanced and indigenous populations in Lake Wylie near Plant Allen. Duke Power, Charlotte,NC. Duke Power. 200lb. Assessment of balanced and indigenous populations in Mountain Island Lake near Riverbend Steam Station. Duke Power, Charlotte,NC. Duke Power. 2004a. Assessment of balanced and indigenous populations in Lake Norman near Marshall Steam Station. Duke Power, Charlotte,NC. Duke Power. 2004b. Assessment of balanced and indigenous populations in Lake Wylie near Plant Allen. Duke Power, Charlotte,NC. L-1 Duke Power. 2004c. Assessment of balanced and indigenous populations in Mountain Island Lake near Riverbend Steam Station. Duke Power, Charlotte,NC. Duke Power Company. 1994. Assessment of balanced and indigenous populations in Lake Norman near Marshall Steam Station. Duke Power Company, Charlotte,NC. Hayes, DB, CP Ferrier, and WW Taylor. 2012. Active fish capture methods. Pages 267-304 in AV Zale, DL Parrish and TM Sutton, editors. Fisheries Techniques, 3"1 edition. American Fisheries Society, Bethesda, MD. Mecklenburg County Department of Environmental Protection (MCDEP). 2003. Lake monitoring data summary for 2001-2002. Charlotte,NC MCDEP. 2007. Lake monitoring report for 2007. Charlotte,NC. Milligan,MR. 1997. Identification manual for the Oligochaeta of Florida. Vol. 1. 187 pp. Neumann, RM, CS Guy, and DW Willis. 2012. Length, weight, and associated indices. Pages 637-676 in AV Zale, DL Parrish and TM Sutton, editors. Fisheries Techniques, 3'edition. American Fisheries Society, Bethesda, MD. North Carolina Department of Environment, and Natural Resources (NCDENR). 2003. Basinwide assessment report; Catawba River Basin. North Carolina Department of Environment and Natural Resources Division of Water Quality. 203pp. NCDENR. 2008. Lake and reservoir assessments; Catawba River Basin. North Carolina Department of Environment and Natural Resources Division of Water Quality. Environmental Sciences Section. 21pp NCDENR. 2013. Standard operating procedure. Biological monitoring: stream fish community assessment program. NCDENR, Division of Water Resources, Environmental Sciences Section, Biological Assessment Branch. Raleigh, NC. Rudstam, LG, JM Jech, SL Parker-Stetter, JK Horne, PJ Sullivan, and DM Mason. 2012. Fisheries acoustics. Pages 597-636 in AV Zale, DL Parrish and TM Sutton, editors. Fisheries Techniques, 3'h edition. American Fisheries Society, Bethesda, MD. L-2