HomeMy WebLinkAboutNC0025496_Toxicity Report Form 2016_20160531 CITY OF LINCOLNTON WASTEWATER TREATMENT PLANT
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'et;tOl l" i"``
May 26,2016 RECEIVED/NCDEQ/DWR
MAY 31 2016
Teresa Rodriguez Water Quality
NPDES COMPLEX UNIT Permitting Section
1617 Mail Service Center
Raleigh,NC 27699-1617
Dear Ms. Rodriguez,
Enclosed is a copy of the May 2016 Fathead Minnow Effluent Toxicity Test conducted by the City of
Lincolnton Wastewater Treatment Plant, NPDES permit number NC0025496. The Fathead Minnow
Toxicity test was requested to be performed for our pending NPDES Permit Renewal. The City of
Lincolnton passed the Fathead Minnow test. I believe this latest test should complete the required 4
alternate species toxicity testing requirement for our NPDES permit renewal.
If you have any questions or need any additional information,please do not hesitate to contact me at 704-
736-8960 or by e-mail at: donaldburkey(a,ci.lincolnton.nc.us.
Sincerely,
Donald A. Burkey,Jr.
WWTP Superintendent
City of Lincolnton
(704)736-8960
608 WEST HWY. 150 BY-PASS • P.O. Box 617 • LINCOLNTON, NORTH CAROLINA 28093-0617
PHONE (704) 736-8960 • FAX (704) 732-6137
A
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date:5/17/2016
Facility: ',colnton WWTP NPDES#NCO() 25496 Pipe#: 001 County: Lincoln
La. 1.101t= Comments
xAli The 5.5%dilution is not included
Signature of Operator in R- . .le Charg in the final results due to
x ' 2 statistical inversion in the 5.5%dilution.
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh,NC 27699-1621
Test Initiation Date/Time 5/3/2016 3:00 PM Avg Wt/Surv.Control[ 0.593 Test Organisms
%Eff. Repl. 1 2 3 4 r Cultured In-House
Control Surviving# 9 10 10 10 %Survival 97.5 r Outside Supplier
Original# 10 10 10 10
WUoriginal(mg) 0.471 0.621 0.631 0.595 Avg Wt(mg) 0.580 Hatch Date: 5/2/16
5.5 Surviving# 9 10 10 10 %Survival 97.5 Hatch Time: 3:00 pm CT
Original# 10 10 10 10
Wt/original(mg) 0.379 0.516 0.496 0.386 Avg Wt(mg) 0.444
8.25 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.546 0.492 0.554 0.556 Avg Wt(mg) 0.537
11 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10 RECEIVEDINCDEQIDWR
Wt/original(mg) 0.641 0.433 0.600 0.571 Avg Wt(mg) 0.561
16.5 Surviving# 10 10 10 10 %Survival 100.0 MAY 31 2016
Original# 10 10 10 10
Wt/original(mg) 0.543 0.498 0.514 0.498 Avg Wt(mg) 0.513 Water Quality
Permitting Section
22 Surviving# 10 10 9 10 %Survival 97.5
Original# 10 10 10 10
Wt/original(mg) 0.547 0.591 0.480 0.626 Avg Wt(mg) 0.561
Water Quality Data Day
Control 0 1 2 3 4 5 6
pH(SU)Init/Fin 8.17 / 7.93 8.09 1 8.13 8.12 / 8.00 8.10 / 7.84 8.25 / 8.01 8.07 / 7.91 8.12 / 7.90
DO(mglL) Init/Fin 7.76 / 6.42 6.92 / 7.62 6.99 / 7.28 I 7.70 / 7.05 7.64 / 7.70 8.15 / 7.48 8.00 / 7.10
Temp(C)Init/Fin 24.5 / 24.9 24.7 / 25.1 24.8 / 25.0 25.6 / 24.9 25.2 / 25.8 24.7 / 25.8 25.3 / 24.8
High Concentration o 1 2 3 4 5 6
pH(SU)Init/Fin 7.98 / 7.91 8.04 / 8.00 8.01 / 8.00 7.98 / 7.91 8.06 / 7.95 8.06 / 8.05 8.12 / 8.06
DO(mg/L) Init/Fin 7.76 / 6.31 6.93 / 7.74 6.89 / 6.95 7.68 / 7.00 7.76 / 7.48 8.06 / 7.72 8.01 17.29
Temp(C)Init/Fin 25.9 / 24.7 25.2 / 24.9 24.4 / 25.8 25.3 / 24.7 24.9 / 25.9 24.4 / 25.8 25.4 125.0
Sample 1 2 3 Survival Growth Overall Result
Collection Start Date 5/2/2016 5/4/2016 5/5/2016 Normal rl Fl ChV >22
Grab Horn.Var. rl Fl
Composite(Duration) 24.0 24.0 24.0 NOEC 22 22
Hardness(mg/L) 34 38 48 LOEC >22 >22
Alkalinity(mg/L) 79 105 116 ChV >22 >22
Conductivity(umhos/cm) 566 770 986 Method Steel's Dunnett's
Chlorine(mg/L) <0.1 <0.1 <0.1
Terrp.at Receipt(CC) 0.4 0.3 1.4 Stats Survival Growth
Conc. Critical Calculated Critical Calculated
Dilution H2O Batch# 1015 1016 1017 5.5 10 18 2.41 Not Included
Hardness(mg/L) 44 46 44 8.25 10 20 2.41 0.9769
Alkalinity(mg/L) 53 50 50 11 10 20 2.41 0.4195
Conductivity(umhos/cm) 189 167 189 16.5 10 20 2.41 1.5228
22 10 18 2.41 0.4252
DWQ Form AT-5(1/04)
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Client: Li Il Co I rl to n #of Organisms per Chamber: 10 Start Date: 5/3/16 Time: -3 .1,rM
NPDES#: NC0025496 Test Vessel Size: 400 ml End Date: 5/10/16 Time: / I 5
Initiated by: / _/( b Test Solution Volume:,25500 ml
Date/Time Fed: 5/3/16 )(1.) 4U,4 f Temp. of Stock:21"5 "C Randomization: 411 No
Date/Time Born: 5/2/16 3:00 PM Cr #of Reps: 4 Incubator#: 1
Organism Source: Aquatox, Inc. Analyst(s): MR, CD, eirr) -SI--
Transferred by: Fed by:
Date Time Initials Date Time 1 Initials Time 2 Initials
Day 1 5/4 O(t) )1_ Cf2 Day 0 5/3 1/2• L0/' 1
Day 2 5/5 3 'Or r1 /YCZ-/Cr- Day 1 5/4 i(��f0ll/1r c47— 114 Fn, n �
Day 3 5/6 1 ti .� �_ Day 2 5/5 K/1.3--(2/77
Day4 5/7 DY'e/"-%/La\ Day3 5/6 ( ,-1')i).1 :1'L
7.) q
Day 5 5/8 ,72 L 1 Day 4 5/7 I'. -
G~ rr Z---- Day 5 5/8 11 "' ) S "I c1
Day 6 5/9 � � � G.,-%-x > � . � .. ti If)
Day 6 5/9 Cf'• lig ,n )0 1 0 � � (-10
Test Termination Data:
Initials/Signature: � �•��kx�,y, .,. /c r'? a.
End Date: 5/10/16
r �f�
End Time: I /• f
ERITECIIy IIVCaw
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls, Inc.
Chemical and Physical Determinations
Client: Lincolnton NPDES#: NC0025496 Start Date: 5/3/16
Test Organism: Pimephales promelas End Date: 5/10/16
M21 7 r
Day
Concentration: control 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
Soft Synthetic Freshwater
Batch#: i315 16 I I, } 17-,
pH:
pH: Initial 5'.17 7.J r '-1, ' 4' ;C' k �) 5', c 7 y
Final 7 .i,'v j' / `-6 .00 7 5y s=,c 1 -j_r i 7.G
D.O.: Initial 7,7(c% l , .' , ' !
Final L,:.y -7. -7.; ,- 7 U> 7 70 - 1 u 7 i
Temp.: Initial r - . 3 . 4.7 .Z`"I • 1 ?5 is„" :7 ? L t 7
Final L.1.9 G 7f 9 )5 g a`7 5' 2.--t
Conductivity: Initial i L i% I /c57 4 (91 171
Final :ply ,rn(-; .-'1-`i j r 4
Residual Chlorine: --0 1 AU. j /- •r <i 1 C. L. i .-'
Hardness: 1414 `I y L L. i 0
Alkalinity: ?•'7 _;_'} � �J 5 ^7 5
Day
Concentration: 5.50% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial O IC / I`--> ' , ,L�
Final (;;",r'',;;' '7.`i 0 . 7-`i c 5-z.'L'
- J
D.O.. Initial 7.7,;,;,— Lr•"iI; .S-2
Final l, ! -7. 7 -I. -7(1\4\4 Z l 7 `-] ( j -7.
Temp.: Initialr G 3 4-/ , H , '1 , _
Final -:`_l ;' %i-.1• i )-4. 4 =moi• / , 1 )tetL.
Conductivity: Initial 1 ,;;?("j5 ry 10 7) '1( •
Final .;,'-71'5 \ u, ' � ! i "2.; ;=5 .' ` -it,
Residual Chlorine: vi • L ,",,; C•e , j ,. ; 42,.
Day
Concentration: 8.25% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial S G.L �' , �� ! j
Final 1 _ 7(i« ,7 . 7 '796 (? -
D.O.: Initial 7 74"/ '-2) k;�, g . 1-1 (-
Final 7 ' '` , ir't'1 77 i 1-,
Temp.: Initial "7 .- .L/ _'- y - SSM
Final -�.`; ? ? '. ! t
Conductivity: Initial - -
Final 9, ac--;
Residual Chlorine: : , ;} f = . r ,_r ! i
ArtelEITAI 7-AECAF-0,
E NJ RCP NI E L 1-.....13C)12.47-C>Ft I Es
Chemical and Physical Determinations
Page 2 of 2
Client: Lincolnton INIPDES#: NC0025496 Start Date: 5/3/16
Test Organism: Pimephales promelas End Date: 5/10/16
Day
Concentration: 11.0% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial 0-7
Final 1 C1C 1.0,4 J bi 7-41 1 9
D.O.: Initial 01 u
Final t: ,7x---1 —7, r
Temp.: Initial -,cn". L-25 ,2*I • af5— ;2 '1 •'t
Final 5. q % :.?"( •
Conductivity: Initial (4 3L7' 22
Final 649 Li \
Residual Chlorine: < . • ) / < c C • LC. ./ /
Day
Concentration: 16.5% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial Sc5
Final 1. 7.&K‹ . 7. 41' % 3
D.O.: Initial 1.7 ,c, 1 -7. C, 77-7L-I 71,33
Final '77C 7 09 7-01 7
Temp.: Initial „--•••,”)5". LI i • r::: „2-C,
Final ra_sT 6 :;2_ - ?'17vi -)-
Conductivity: Initial ,:9L,) -3 .1 _
Final ;--;)5,.. 3,,E5 7, 317.-3/..
Residual Chlorine: 0 i GO. ) CiZC./
Day
Concentration: 22.0% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial (-4 q.,:, 7.c),
Final -7 ci 31) ? 'ii
D.O.: Initial 7 L r ;2; f ,9-T.; 7 1 q"
Final ,'":;) I -74 (1) 7- '1, S 7T2 7 c,-2
Temp.: Initial "•-tl i9 •
Final 9 7c. y )4. 7 ..;2.(, -47
Conductivity: Initial I 1,t-3 3 -77 -L3
Final -; 'E 1 `-'? 7 5 ii r
Residual Chlorine: C, • , I cj .
Day
Concentration: 100% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: 7 '5 5 .-i7 :
Conductivity:
Residual Chlorine: r ,
c'
•
Hardness: , •
Alkalinity:
7
•
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Lincolnton NPDES#: NC0025496 Start Date: 5/3/16
Test Organism: Pimephales promelas End Date: 5/10/16
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
/ l (../ L G 3 f
Rep A 0 v
Rep 0
JC C:
Rep C 0 ✓ L? C.. " ; �� �.
i `J \c C7 0
RepD 0 Li
Concentration 5.50%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 G. /,�� (` (� 1 6)
Rep 0 C Cv v cy
C."
Rep C 0 J v
Rep D 0 J �} ` / ._ 0i �
Concentration 8.25%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Re A 0 �, L.-/ (' ! '
`� , C 1 0
Rep
•
r � ci.
, i /
RepB 0 U
0 (:2
Rep C 0 �J �'/ �` ^3
Rep D 0
� V (_,
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Lincolnton NPDES#: NC0025496 Start Date: 5/3/16
Test Organism: Pimephales promelas End Date: 5/10/16
Concentration 11.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 i' f C� 0 (�.! L! Ls)
Rep B 0 0U C-' C: (,' j Cl
Rep C 0 .). ( t
(
,! U j l 0Rep D 0T,
Concentration 16.5%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
L/
/�� / ) 7 t
Rep A 0 t.l (/ C. �' U !/
Rep B 0
c,
Rep C 0 12
v `- 6,./
0 71
Rep D 0 , f C:` l,'
Concentration 22.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 U C ty
C, i ()
Rep B 0 () I G^ (.) i- ; V,
Rep C 0 _ ✓ `� •
Rep D 0 `" L i L .? �J
.im E ITEC' INC.
N s
` , ENVIRONMENTAL LABORATORIES
^ A Division of Water Technology and Conrrols, Inc.
FISH LARVAL SURVIVAL!GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
•
Facility: Lincolnton Page: 1 of 2
NPDES #: NC0025496 Outfall:
Date of Test: 5/3/16
Initial weights taken on: H-.:_)::-.k.u, by: =%1--- Organism: Pimephales promelas
Final weights taken on: 1?-;L by: L.
PAN WT. PAN + ORG. WT. OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) #ORG. WT./ORG. (mg) SURV%
— i• , , . / / 9 0..\i.7 i 1 c)
a
B co 31. `-15 �': '1 i 4: ,:. ! f�'; o V -1 /1)U
0
U
c '3'`-1'{•a.0=-I Sou: . 5 u. ?. (C 0. ii 3.1 /P) )
5.50% B -;, 'i � , i_�r /Cr; C'` .6 /e)/ 0
1
•
/Li Li
A �' 3< i x :;,, / K • ,
B i j8.25%
c . . _ 1 i, (I E'S`L /C
'_ z. E I TECH, ®/VC_
, '', ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Con rrols, Inc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Lincolnton Page: 2 of 2
NPDES #: NC0025496 Outfall:
Date of Test: 5/3/16
Initial weights taken on: ±9 - i(.. by: .5.(-. Organism: Pimephales promelas
Final weights taken on: 5-19-1{:, by: S e-
PAN WT. PAN + ORG. WT. OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) #ORG. WT./ORG. (mg) SURV%
ri n
11.0% B Jam'. J .;'-, 5. 1 "7.3 0 0 - .)_) `cti0
C L., ?) .J'-' (0 t-t,Ot) /0 0
D `5;:,) . C ?,L_ -7 ~ 5 .1 1 (0 n, 7I l o U
A ,Q 1,4,. Lt. L 5'37. C'i .`-, I 0 0- z; _ (C
Qj --,;'" ' r) �' - C' '-I (-i j 0 C ,.\-;\G+ 4 l tom' r1
16.5% B ;. =;
D x L L y< . y y JO e' . - 1r=, i
A :-L I :''';fir 4 s n u ,--7 /0 (7-( ''i % (C,'0
22.0% B 4 -' 7' C y ,' . . ;- -i ; re' G .��li ( C'0
C :Thi_ . -- ` - - ('_ 't k'' L -1
?` V I C'
•
MERITECH, INC. MeritechSamplela#: 3(S
ABioassay Sample Chain of Custody
642 Tamco Rd,Reidsville NC 27320
Phone: 1-336-342-4748 Fax:1-336-342-1522
Toxicity Supervisor email: mike.reed@meritech-labs.com Web Site: www.meritech-labs.com
fF • 1.-41(
/ CLIENT INFORMATION
Client: &1 'y C (� L 1 tr1('C�L y47,if'-) 14/0-13 PO#:
Contact Person: iA r1 i--CerLahr /'1;-I,% 8t rr NPDES#: NC (t ...5Y 6
Address: P0 2)j X (c l Phone: 10 4, 7. ;-.. E--q,0
City: �-{f1[tv!ri Pipe#: Qo /
County: Lela/r1 State: AZ_ Zip:/9-S-%-6q3
SAMPLE INFORMATION
Sample Site: L—PF
Sample Type: ❑Grab Composite #of containers:
Sampling Time: Start Date: ,..-)/a 11(.p Start Time: 08e V AM PM
End Date: 5/3 If cc, End Time: OS-0 M ) PM
'Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The """
sample must be<6.0°C upon recei t the labor ry
Collector's Name: Print: -Qjim,r�-2l. Lei 1'`-e-- Signature: c.;_.....,,,,TOXICITY TEST INFORMATION
c----- -
Test Required: ❑Chronic(7 days) Test Organism: ❑ Ceriodaphnia dubia (water flea)
❑Acute(24-48 hours) C .Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: ( i % Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION
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OI
Relinquished by: riAlrAPMAPIIM Date: -. --` {v Time: 61`*_ ' AM (61...)
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Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX XMeritech Pick-up ❑Delivered ❑ Other
"Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED"
ff �� ((// SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: `'G-J _ 5G.F� XL,
/
Received y: / Date: C- -1 ti Time: I -( ..✓ AM �PM
l
Sample Temperatures(°C): U,Lt I V.\-{ I I Sample Condition: -----,4 cA
,.. WHiTE= Laboratory copy YELLOW=Client copy -4Carva.1_,,,*,--T...--A-rw,nk.
MERITECH, INC. Meritech Sample ID#: (/-5'b.C1 j' ti
Bioassay Sample Chain of Custody
,,,A-__.
642 Tamco Rd,Reidsville NC 27320
Phone: 1-336-342-4748 Fax: 1-336-342-1522
Toxicity Supervisor email: mike.reed@meritech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: �' v irlc.-C.'hi jr) �,j , T PO#:
Contact Person: �Tc r i-�-�t- LCtty- / )r i-jL',r(= L) NPDES#: NC OC) ;--5 9,
Address: Ct Q4'`1 (019 ✓ -
Phone: 7014. ?&(c. E-56 0
City: 1-.1`v\ t.D 1,,d-Din Pipe#: Cd
County: J,_.Ey. e 0(r. State: AIC- Zip: tS12)C13
SAMPLE INFORMATION
Sample Site: C P H
Sample Type: ❑Grab VI.Composite #of containers: 1.)
Sampling Time: Start Date: .l 4 111.0 Start Time: J 8.0S- CAM PM �.�
End Date: 5151 Ilp End Time: FcCS AM PM
'-`"Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be<6.0°C upon receiat at the lab story*'*
.2�
Collector's Name: Print: ��4\Y\��: �1�-Y\e Signature:
TOXICITY TEST INFORMATION ��/J
Test Required: ❑Chronic(7 days) Test Organism: ❑ Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) tX,pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: 1) % Test Concentrations(if multiple dilutions):
Comments:
n s1 SHIPPING INFORMATION /
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Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX ,Meritech Pick-up ❑Delivered El Other
**Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED*'
SAMPLE RECEIVING (Laboratory Use Only)
f r
�/�
Relinquished by:- i '� !V I/'"7-4-'-
��\
Received by: � ✓, /// . Date: � / Time: J � AM PM,
/
Sample Temperatures(°C): . 3 I L. I I Sample Condition: `r 0 1%
Vt'i-f T E= Laboratory copy Y-ELLCV r
=Cif6 t:copy
MERITECH, INC. MeritechSample ID#:O)'J `( 6:::'5F)
Bioassay Sample Chain of Custody
rip-1-*„.......
642 Tamco Rd,Reidsville NC 27320
Phone: 1-336-342-4748 Fax: 1-336-342-1522
Toxicity Supervisor email: mike.reed@meritech-Iabs.com Web Site: www.meritech-labs.com
. CLIENT INFORMATION
Client: C(- i.' 0 C Lir C 0,4-1'7bn IA/11:,11' PO#:
Contact Person: `� y1 i Per l ihr / b"`yl (7 .2v1/..t.)) NPDES#: NC C(),!.Sq 9 f-,
Address: fV � A L%(7Phone: '704 75(^.. c�-i L
L
City: N((11rt hi Pipe#: ere))
County: C_'jY‘LC 1 Yl State: Ai Zip: 38-6:4 3
SAMPLE INFORMATION
Sample Site: �--
Sample Type: ❑Grab omposite #of containers:
Sampling Time: Start Date: 5!5 li Start Time: ult.,;,,, (N. % PM r •
) �74
End Date: 5 I'll t, End Time: I CC,C.", �A ., PM
***Triple rinse sample container with sample before`Mho. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice.1 ,The sample must be<6.0°C upon receipt at the laboratory
77L't' r 1
Collector's Name: Print: \(� t'T� L
( -�-I f `- Signaturj(�1 —`C
TOXICITY TEST INFORMATION (".....j
Test Required: ❑Chronic(7 days) Test Organism: ❑ Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) 71.Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: 1 1 % Test Concentrations (if multiple dilutions):
Comments:
ii SHIPPING INFORMATION
Relinquishe y: i '---� Date: 45 -(C:--/ . Time: J 0 (AIy�
i PM
./ 7
Receive j /nf 't - ..'fes`r Date: _ ,/ 6 Time: 16', 6:5-21) r% /P-M
Relinquished by: /j '/ //f/f I i l� Date: 6,- i, Time: --!."47:1-.6.2) AM (rlO
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Relinquished by: Date: Time: AM PM
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Sample Temperature/ (°C):
Method of Shipment:❑ UPS ❑ Fed EX XMeritech Meritech Pick-up ❑Delivered ❑ Other
-Samples shipped on Friday mast be FedEx and must be c;eariy labe;ed for Saturday dellve-y,NO SIGNATURE REQUIRED"
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
,k.,—/'I
Received by: 000 Date: ,-L Vi, 1 % Time: '(--{C. AM '�1V1,-
Sample Temperatures(°C): 1 l i / L / Sample Condition: �,1./ ,_a.
WRITE= Laboratory cope YELLOW=Client copy "�-"""t'
Title: lincolnton
File: lincolnton Transform: NO TRANSFORMATION
Shapiro - Wilk' s Test for Normality
D = 0. 0568
W = 0 . 9114
Critical W = 0.8680 (alpha = 0. 01 , N = 20)
W = 0. 9050 (alpha = 0 . 05 , N = 20)
Data PASS normality test (alpha = 0. 01) . Continue analysis.
Title: lincolnton
File: lincolnton Transform: NO TRANSFORMATION
Bartlett ' s Test for Homogeneity of Variance
Calculated B1 statistic = 6 .2684 (p-value = 0. 1800)
Data PASS B1 homogeneity test at 0. 01 level . Continue analysis.
Critical B = 13 .2767 (alpha = 0.01, df = 4)
= 9 .4877 (alpha = 0. 05, df = 4)
Title: lincolnton
File: lincolnton Transform: NO. TRANSFORMATION
ANOVA Table
SOURCE DF SS MS F
Between 4 0. 0105 0.0026 0.6965
Within (Error) 15 0 . 0568 0. 0038
Total 19 0. 0673
(p-value = 0.6061)
Critical F = 4 .8932 (alpha = 0.01, df = 4, 15)
= 3 .0556 (alpha = 0.05, df = 4, 15)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0. 05)
Title: lincolnton
File: lincolnton Transform: NO TRANSFORMATION
Dunnett's Test - TABLE 1 OF 2 Ho:Control<Treatment
TRANSFORMED MEAN CALCULATED IN SIG
GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0.05
1 control 0 .5795 0.5795
2 8 .25 0.5370 0 .5370 0. 9769
3 11 0.5613 0.5613 0 .4195
4 16 .5 0.5132 0.5132 1.5228
5 22 0 . 5610 0 .5610 0.4252
Dunnett critical value = 2 .3600 (1 Tailed, alpha = 0. 05, df = 4, 15)
Title: lincolnton
File: lincolnton Transform: NO TRANSFORMATION
Dunnett' s Test - TABLE 2 OF 2 Ho:Control<Treatment
NUM OF MIN SIG DIFF % OF DIFFERENCE
GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL
1 control 4
2 8 .25 4 0.1027 17 .7 0. 0425
3 11 4 0.1027 17 .7 0.0183
4 16 .5 4 0.1027 17.7 0 .0663
5 22 4 0.1027 17.7 0. 0185
Title: lincolnton - survival
File: lincolnsury Transform: NO TRANSFORMATION
Shapiro - Wilk' s Test for Normality
D = 0.0225
W = 0.6683
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data FAIL normality test (alpha = 0. 01) . Try another transformation.
Warning - The first three homogeneity tests are sensitive to non-normality
and should not be performed with this data as is.
Title: lincolnton - survival
File: lincolnsury Transform: NO TRANSFORMATION
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless .
Title: lincolnton - survival
File: lincolnsury Transform: NO TRANSFORMATION
Steel ' s Many-One Rank Test - Ho: Control<Treatment
MEAN IN RANK CRIT. SIG
GROUP IDENTIFICATION ORIGINAL UNITS SUM VALUE DF 0 .05
1 control 0. 9750
2 5.5 0.9750 18. 00 10. 00 4 . 00
3 8.25 1.0000 20. 00 10. 00 4 . 00
4 11 1 .0000 20. 00 10. 00 4 . 00
5 16 .5 1. 0000 20. 00 10. 00 4 . 00
6 22 0.9750 18. 00 10.00 4 . 00
Critical values are 1 tailed ( k = 5 )