NC0026051_Lab analyses_20041231

Laboratory Benchsheets
and Statistical Analyses
’ n uiiiin.ni.aL cMing solutions, Inc.Page 1 of 1
NC Modification- February. 1998) Species: Ceriodaphnia dubia
____________Project# Control # 1 Date OZ '2$-D^
5
4 % Adults having 3'a Broods (> 80%):
3 % Morality (< 20%);
2
0.U^-
Date Analyst
0
1 Feeding
2
3 Feeding
4 Feeding
5 Renewal 2
6 Feeding
7 Termination
1 2 3 4 7 8 9 10 1211
Adult mortality L L C_L_V v_L L
Young produced5
15^
Adult mortality v_L_LL l_
6 or 7 Young produced >5 iSnisnnnn13
Total young produced 32 32 3U3%3‘-I2133 333M31
Final Adult Mortality u L.L L_
53
Mean Offspring'Female
(> 15 offspring/surviving female'):
% CV (< 40.0%):
Test initiation, renewal,
feeding, or termination time
Initiation
Acceptance Criteria:
% of Male Adults (<20%):
Test information:_______
Randomizing template:
Incubator number and shelf
location:_________
YCT batch:
Selenastrum batch:
ABS
ABS
Test organism information:
Organism age:______________
Date and times organisms
were bom between:
Organism source:
Transfer bowl information:
Daily renewal information:
Day
ICZ'ZS'OI
031)2-^
i5
Day
2
< 24-hours old
OZ-zM-oH '‘Seto-vc n'S
2 Cl
pl-27-6 4
OTo
l(X>7.
07
32.6
igitub- CYWd
Du fCTYo-r—. Cd .
1 CONTROL
Control water batch used
_________(SSVV)
02-2.1.0^
O2-Zl.t>‘-I
os-sli.cC]-
Renewal Two______
k.-ye
'left
25. T
Final
I n i t i ation
I W
l.&zI
75.Z
Final Initial
Renewal One______
zSzr
FinalInitial
Survival and Reproduction Data
Replicate number
153
_______________I X I X I x
\oie An X in the upper right comer of the number of young produced indicates the presence of two broods.
11/
Parameter_____
Analyst ___________
pH(S.U.)_______________
DO (mg/L)______________
Conductivity (pmhos/cm)
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO3/L)
Temperature (°C)
C4A li
3-^ Il
3 3
15~i [
31 If
H3 I
Initial |
ontrol Information, North Carolina Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013
-----------------5Iethod 1002.0. NC Modification- February, 1998) Species: Ceriodaphnia dubia
Test grouping information: Project # Control 1 Date OZ-25-0^
6 -------------------- ---------------------------------- -------------------
1-7 ?-
e>-i
-153c
/X IX15 x
L_
13/iX
o-z.-1-i-M A-ts__________
pH =2. SU Temperature=
iX
uqu
Renewal I
o ezH
cWl,
IQ3Z
QtoZU
35C)(o
X for 3rd Broods
5
Page 1 of 1‘ Environmental Testing Solutions, Inc.
Comments:
Sample information:
^5 554-
on-c<0.10
Test Concentration: tyO'l'
1211910623451
L_Adult mortality L c_c L.C_
Young produced5 n
Adult mortality I—c_L L-VL
15 nYoung produced 1$6 or 7 iszo
3334343134Total young produced 34353S333333
L- ILFinal Adult Mortality oLL
L_
Collection start date:
Collection end time:
Grab/Composite
(duration):_____________
Temperature (°C) upon
receipt:________________
Physical characteristics:
ETS project number:
ETS sample number:
Client:
Facility:
Test concentration chemistry:
Parameter
Test results:_______
% Mortality:_____________
Mean Offspring/Female:
% Reduction from control
Calculated t:_____________
Tabular t:_______________
Pass or Fail
^3.
30
Survival and Reproduction Data
______Replicate number
7
TCriH
O^OZ^S-OZ-
Sample 2
c&zo
•zvI-^ov/L coKfosiTe
Analyst
pH (S.U.)_______________
DO (mg/L)______________
Conductivity (pmhos/cm)
Temperature (°C)
NPDES#: NC
County:_____
Outfall/Pipe:
04622^-01
Full-strength chemistry:
Parameter
pH (S.U.)
DO (mg/L)
Conductivity
(pmhos/cm)
Chlorine
(mg/L)
Dilution preparation:
Test concentration:
mL Sample:__________
mL Dilution water:
Day
2
Sample 2
IS-?
3-3
I n itiation
3 37
8-0
Final
CAA
Initial
8
L_
3-52-
S-Z
j ?
2S-7
Initial
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test
(EPA-821-R-02-013 Method 1002.0, NC Modification- February, 1998)
Species: Ceriodaphnia diibia
Parted with Control # / Date 0
QO 2^ —
00/
7- 5 3
8- 4
Initial
00-
H^-3 s'
Sample 1
8-S
Renewal One
S-o
7^.3
Final
Per
4/zo"
L
13*
Renewal Two
Final
Sample 1
01^-c^
O^io
QPCrpctS'tL
0-3‘O
^lOaJdz-QS
pWicUa)
L_
>.Die: An X in the upper right comer of the number of young produced indicates the presence of two broods.
(5*|3X 13*
35.000 34.000 35.000 35.000 34.000 34.000 39.000 34.000
t-Stat MSD
-2.111 2.508 2.476
Dose-Response Plot
45
40
35
15
10
5
s
Ditiliain_02-2.!' •"■J
2/25/2004
3/3/2004
Mean
32.833
34.917
_1_____
32.000
34.000
37.000
37.000
N-Mean
1.0000
1.0635
Mean
32.833
34.917
N
12
12
GET - Durham County Triangle WWTP
DMR-Discharge Monitoring Report
CD-Ceriodaphnia dubia
l-Tailed
Critical
■ ■ 1-tail, 0 01 level
ol significance
2
36.000
33.000
32.000
33.000
Environmental Testing Solutions, Inc.
Statistical Analyses
Test ID:
Lab ID:
Protocol:
5____
33.000
Conc-%
D-Control
90
6____
38.000
8_____
34.000
10____
34.000
Auxiliary Tests
Shapiro-Wilk's Test indicates normal distribution (p > 0.01)
F-Tesl indicates equal variances (p = 0.22)
Hypothesis Test (1-tail, 0.01)
Homoscedastic t Test indicates no significant differences
Treatments vs D-Control
Start Date:
End Date:
Sample Date:
Comments:
Conc-%
D-Control
D-Control
90
90
3____
32.000
7____
30.000
9____
31.000
df
1.22
4
27.000
Skew
-0.010316
Kurt
1.00503635
0
Ceriodaphnia Survival and Reproduction Test-Reproduction
CDPFCRNC Sample ID:
ETS-Environ. Testing Sol. Sample Type:
CHRONIC-EPA-82l-R-02-013 Test Species:
c 30
o
O 25
I- u cr
Statistic Critical
0.96811521 0.884
2.1425662__________5.31966734_________________
MSDu MSDp MSB______MSE F-Prob
2.47564926 0.0754(XM9 26.M16667 5.84469697 0.04638283
Transform: Untransformed
Min_______Max______CV%
27.000 38.000 8.598
32.000 39.000 5.524
Rcvchvcd by:
I );i v
I 2 3 5 6 7 8 9 10 11 12
S( onlrol 17 19 15 12 16 19 13 16 18 15 17 15
7 15 17 17 15 17 19 17 18 13 19 17 10018 32.8 8.6
32 36 32 27 33 38 30 34 31 34 34 33
9(1%17 17 20 16 17 16 19 17 20 15 19 19
7 20 15 15 18 18 19 15 17 19 19 18 10014 34.9 5.5 -6.3
Total 37 32 35 34 35 35 34 34 39 34 37 33
l>i<rluun_0- 25 H-l
Number of young produced by replicate niiinbcr
4
Clienl:
l est dates:
Project number:
I'ercviit reduction from
control (%)
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia
Quality Control
Verification of Data Entry, Calculations, and Statistical Analyses
Averuge
reprodu ction
(on's|iriiig/fcmnlc)
Dunnell's MSI) value:
PM SI):
< '(iiiccutration
(%)■Survivol
(%)
Not applicable
Total
5
2.476
7.5
( l~. r- Din liani (•<.untv Triangle WWTP
l~ehriiar;.' 25 - Marell 03, 2004
1094
Cocllicicnt of
vnrintion
(%)
MSD = Minimum Significant Difference
PMSD = Percent Minimum Significant Difference
PMSD is a measure ol test precision. The PMSD is die minimum percent difference between die control and treatment that can be declared
statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc.
chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 10.0% from the control.
Lower PMSD bound determined by USEPA (10th percentile) = 11 %.
Upper PMSD bound determined by USEPA (90°‘ percentile) = 37%.
The lower and upper bounds were calculated by die USEPA using 393 tests conducted from 33 laboratories for Ceiiodaphnia reproduction in
chronic reference toxicant tests.
I LSI TA. 2()()(). I inderstandim- and Accounting for Mcdiod Variability in Whole Effluent Toxicity Applications Under die National Pollutant Discharge Elimination Program. EPA-833-R-OO-OO3. I IS Environmental Protection
Agcin y, < 'incinnati, (>11
May 27, 2004
RE:ETS PROJECT NUMBER: 1260
Dear Mr. Thrower:
Test Procedure
EPA-821-R-02-013TGP3B PASS
If you have any questions concerning these results, please feel free to contact me.
If this test was performed as an NPDES requirement or by Administrative Letter, please
enter a P on the Effluent Discharge Monitoring Form (MR-1) for the collection date
May 17, 2004 using the parameter code TGP3B.
Additionally, please sign and submit the enclosed DWQ Aquatic Toxicity Form (AT-1)
to the following address. The original AT-1 form must be mailed by June 30, 2004.
North Carolina Certificate Numbers: Biological Analyses: 37. Drinking Water: 37786, Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
Enclosed are toxicity test results for samples from the Durham County Triangle
WWTP received by Environmental Testing Solutions, Inc. May 19 through
May 22, 2004.
Parameter
Code
North Carolina Ceriodaphnia Chronic Effluent
Toxicity Procedure - December 1985, Revised:
February 1998
{Ceriodaphnia Pass/Fail Toxicity Test)
Final
Result
Mr. Link Thrower
Chemical & Environmental Technology, Inc.
102-A Woodwinds Industrial Court
Cary, NC 27511
EPA Method
Number
PO Box 7565
Asheville, NC 28802
North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
Sincerely,
Jim Sumner
Laboratory Director
Environmental Testing Solutions, Inc.________
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Date:May 27, 2004
Pipe #: 001 County: Durham
Comments: .
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent
90%
FailPass
X
05-19-04Test Start Date:
7.40
05-20-04Sample 2
24-h
24 -h
34
625
1.1
•2 =
to
Control
High Cone.
DO (mgTL)pH (S.U.)7-dajsCeriodaphnia dnbia Duration:Organism Tested:
D ICQ form AT-1 (3/87) rev. 11/95
Duration
T
Alkalinity (mg CaCOj/L)
Hardness (mg CaCO3/L)
Concentration (%)
Mortality (%)
D.O. (mg/L)
Control
Treatment 2
Method of Determination
Trimmed Spearman Karber
Probit
Other:
Conductivity (pmhos/cm)
Total Residual Chlorine (mg/L)
Sample Temp, at Receipt (°C)
Chronic Test Results
Calculated t:
Tabular t:
% Reduction:
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
PO Box 7565
.Asheville, NC 28802
pH (S.U.)
Control
Treatment 2
3 3
Treatment 2
30.4
I I
I
co 1
j
Morality
Control
0.0
Average
Reproduction
Control
29.8
Effluent Percentage
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
Control Organisms_________
Number of Young Produced
Adult Survival: (L)ive, (D)ead
2
35
L
2
33
L
7.48
7.55
5
28
T
5
31
L
■
33
L
7.9
8.0
7
28
L
7
28
T
8
31
L
9
33
L
10
31
L
12
30
L
Project:
Samples:
1260
040519.04,040522.04
43
173, 162,
176
I 5
P
I
32
T
8.0
8.1
3
28
L
3
29
L
4
30
L
4
31
L
2nd Sample
7.43
7.31 7.59
6
24
L
9
32
L
10
26
L
11
27
L
11
25
L
12
34
T"
-0.488
2.508
-2.0
697
<0.10
1st Sample
8.0
8.4
1st Sample
7,15
7.12
1st Sample
7.8
TT
8
32
T Treatment 2
0.0
Control CV
<0.10
0.9
1st Sample
7,33 7 14
74? 7.20
1
32
L
7.8
7?8
2nd Sample
8.1
7.9
10.1
% control orgertisrrB
produemg Jrdbrood
100
LC?0/Acute Toxicity Test
(Mortality expressed as %, combining replicates.)
LC50=
95% Confidence Limits
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Sample Type/Duration
Grab
Sample 1 |
Sample 2
Comp
X
Environmental Testing Solutions, Inc.
Phone: [phone]
Fax: [phone]
E-mail: JimSumnerfajaoLcom
Collection (Start) Date:
Sample 1 05-17-04
Facility: CET NPDES #: NC- 0026501
Durham County - Triangle WWTP
Laboratory Performing T est: Environmental Test^igSolutipas, Inc. A
Signature of Operator in Responsible Charge: y
Signature of Laboratory Supervisor: (77-
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC Date-May 27, 2004■50
Pipe#: 001 County: Durham
Comments: .
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent
Pass Fail
X
Test Start Date:05-19-04
7.14
7.59
Sample 2 05-20-04
Dura lien
24-h
24-h
Q
34
=~=
Control
High Cone.
DO (mg/L)pII(S.K)Organism Tested:Ceriodaphnia dubia Duration:7-days
mVQforrn AT-1 (3/87) rev. 11/95
I
Concentration (%)
Mortality (7c)
D.O. (mg/L)
Control
Treatment 2
Method of Determination
Trimmed Spearman Karber
Probit
Other:
Sanple 1
Sanple 2
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO3/L)
Conductivity (pmhos/cm)
Total Residual Chlorine (mg/L)
Sanple Tetip. at Receipt (°C)
Clironic Test Results
Calculated t:
Tabular t:
% Reduction:
PO Box 7565
Asheville, NC 28802
1
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
I
pH (S.U.)
Control
Treatment 2
I
Treatment 2
30.4
1 1
_u
■9.
Average
Reproduction
Control
29.8
7.8
tT
1
32
T
7.8
8.1
2
35
L
3
29
L
3
28
L
4
31
L
5
31
L
6
24
L
8
32
L
9
33
L
11
25
T
12
30
L
Project:
Samples:
Morality
Control
0.0
1260
040519.04, 040522.04
-0.488
2.508
-2.0
Control Organisms_________
Number of Young Produced
Adult Survival: (L)ive. (D)ead
1
32
T
4
30
5
28
L
6
33
l”
7,9
?0
7
28
L
7
28
L
8
31
T
9
32
T
io
26
L
10
31
L
11
27
L
43
17.'. 16i
176
1st Sample
8.0
8.1
2
33
T"
1st Sample
8.0
8.4
1st Sample
7,33
7.19 7.20
1st Sample
7.15 | 7.48
7.12 7.55
2nd Sample
7.43 7.40
7.31
12
34
T
2nd Sample
8.1
7.9
697
<0.10
1.1
Treatment 2
0.0
Control CV
10.1
conVo] organjsns
producing 3rd brood
100
Sample Type/Duration
Grab Comp.
x~
x-
Environmental Testing Solutions, Inc.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates.)
LC50=
9591 Confidence Limits
to
= 5 j
.........
625
<0 10
09
Collection (Start) Date:
Sample 1 05-17-04
Facility: CET NPDES #: NC- 0026501
Durham County - Triangle WWTP
Laboratory Performing Test: Environmental Testjing Solutions, Jnc-
Signature of Operator in Responsible Charge: \ lIjUOT? <
Signature of Laboratory Supervisor:
Effluent Percentage 90%
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
Laboratory Benchsheets
and Statistical Analyses
lnviruiiuiciiiai iebimg ouiuiiuiib, inc.
ControlProject #
I25T-oc
% Adults having 3fd Broods (> S0%):
121^1
% Mortality (< 20%):12^0
AnalystDay
0
Feeding1
Renewal I2
FeedingJ
Feeding4
Renewal 25
Feeding6
Termination7
12111096852341
Adult mortality
Young produced Cs5KoiV
Adult mortality L_L_L__k_
/^76 or 7 Young produced 15isIxf15I
2'7Total young produced 2S 32 3133303532
L_Final Adult Mortality L,LL_L_L
Test organism information:
Organism age:________________
Date and times organisms
were bom between:___________
Organism source:_____________
Transfer bowl information:
Daily renewal information:
Date
■Mean Offspring/Female
(> 15 offspring/surviving female):
% CV (< 40.0%):
.Acceptance Criteria:
% of Male Adults (< 20%):
Test information:_______
Randomizing template:
Incubator number and shelf
location:_________
YCT batch:
Selenastrum batch:
ABS
A3S
I boo.
OT
/O./'O
26. I
Initial
Survival and Reproduction Data
___________Replicate number
7
CM
I--63
3^
^3
Initial
2^-L
Initial
05-23-0^
OS-21
05-22-0^
-23>-C^
OS -zs-o]
05 -Z-Us-Ol
Test initiation, renewal,
feeding, or termination time
ln,,,a,ion I |M O
0340?
CT04
Qz/Z-g
CASS
0?SO
2 Cl
04-ZWJ
Control water batch used
__________(SSW)_________
Q5 A 1-03
n^m-oA
os- k\-o4
OM
-1(5
\^Z-nip
33
KEK
-A---
J
Day
2
Initiation
26.2.
Final
Renewal One
-I aA ""
.8.3
Final
1302
Control Information, North Carolina Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013
Method 1002.0, NC Modification- February, 1998) Species: Ceriodaphnia dnbia
US
U-
Parameter
Analyst
pH (S.U.)_______________
DO (mg/L)______________
Conductivity (pmhos/’em)
Alkalinity (mg CaCOj/L)
Hardness (mg CaCOj/L)
Temperature (°C)
Renewal Two
•z^/3
Final
21^
7 I
/2*
20*
I Date
>3*
< 24-hours old______
O-^-iS-oA I'ccO
I3A
c5-ii-o4 Pk-r__________________
pH =~7.G~7 SU Temperature= |°C
1^
\ for 3'd Broods
2^X
Test grouping information:
- -ScruTlctt /^owjmH-P
" ~T^i W-- 3. S'-zc
J Du(Ln4/-rT Co
2 C<QvvVn - &2
1 CONTROL
Page 1 of 1■ Environmental Testing Solutions, Inc.
LaJUO I p
Comments:
Sample information:
Ml
<o.iO
1 M^SZ2,CH J
qp'l.Test Concentration:
119106834512
Adult mortality LLU-L-
/TYoung produced x 55/H /f1 (o 11
t_Adult mortality c_L_L_1_v_
/GYoung produced6 or 7 /5 H11
Total young produced 32.2.52C322^31 253 I 33 3133
L_Final Adult Mortality c_k c_c3
Note: An X in the upper right comer of the number of young produced indicates the presence of two broods.
L
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test
(EPA-821-R-02-013 Method 1002.0, NC Modification- February, 1998)
Species: Ceriodaphnia dubia
Parted with Control #/_ Date (J'S
ZG'
Test concentration chemistry:
Parameter
Full-strength chemistry:
Parameter
Client:
Facility:
ETS project number:
ETS sample number:
Test results:_____________
% Mortality:____________
Mean Offspring/Femalc:
% Reduction from control
Ca 1 cu 1 atcd t:_____________
Tabula r t:_______________
Pass or Fail
Collection start date:
Collection end time:
Grab/Composite
(duration):_____________
Temperature (°C) upon
receipt:________________
Physical characteristics:
I
Qt-IOQ ll.C)!'
Q-A -<2
Analyst
pH (S.U.)_______________
DO (mg/L)______________
Conductivity (pmhos/cm)
Temperature (°C)
Day
2
NPDES#: NC
County:_______
Qutfall/Pipe:
002^50)
061——_
pH (S.U.)
DO (mg/L)
Conductivity
(pmhos/cm)
Chlorine
(mg/L)
Dilution preparation:
Test concentration:
mL Sample:
mL Dilution water:
IW-I-
30
Survival and Reproduction Data
_________Replicate number
7
Sample 1
TH
a.q
zo.»o
o*\
8-Q
5g <3
26,4
Initial
8.(
531
2H .1
Initial
Sample 1
b 5'11 -c4
ll
Renewal Two
ec
QI.
30.1
25- 3
Final
2^.^
Final
Sample 2
-t34
84
12
CA\
1-?'
■ 1.1
2S-3
Initial
Renewal One
-x-ss
Final
Initiation
T-ZD
1
2^
c.
Sample 2
05-26-C4
065T)
i.rc
21^
/Lx
29.000 31.000 31.000 26.00033.000 28.000 31.000 32.000
MSDt-Stat
-0.488 2.508 2.998
Dose-Response Plot
40
35 ■■
30
o 25 ;
10
5
7_____
28.000
5/19/2004
5/26/2004
_1_____
32.000
27.000
32.000
25.000
24.000
25.000
GET - Durham County - Triangle WWTP
DMR-Discharge Monitoring Report
CD-Ceriodaphnia dubia
1-Tailed
Critical
1-tail, 0.01 level
.. of significance
Environmental Testing Solutions, Inc.
Statistical Analyses
Test ID:
Lab ID:
Protocol:
5
28.000
Auxiliary Tests_________________________________
Shapiro-Wilk's Test indicates normal distribution (p > 0.01)
F-Test indicates equal variances (p = 0.85)
Hypothesis Test (1-tail, 0.01)_______________________
Homoscedastic t Test indicates no significant differences
Treatments is D-Control
Start Date:
End Date:
Sample Date:
Conunents:
Conc-%
D-Control
D-Control
90
90
Conc-%
D-Control
90
2_____
35.000
30.000
33.000
34.000
6
24.000
N
12
12
9_____
33.000
MSE
8.5719697
10____
31.000
df
1,22
3
28.000
4
30.000
8____
32.000
Mean
29.833
30.417
N-Mean
1.0000
1.0196
Mean
29.833
30.417
F-Prob
O.63O3528
Skew
•0.44672
Kurt
-0.3596003
0 - -
I
Ceriodaphnia Survival and Reproduction Test-Reproduction
CDPFCRNC Sample ID:
ETS-Environ. Testing Sol. Sample Type:
CHRONIC-EPA-821-R-02-013 Test Species:
Transform: Untransfonned
Mki Max CV%
35.000 10.090
34.000 9.347
•o 20
Q.
- 15
Statistic_______________Critical
0.96249372 0.884
1.12089968____________5.31966734
MSDu MS Dp MSB
2.99811598 0.10G49551 2.04166667
Environmental Testing Solutions, Inc.
Rcvciwcd by:
Diiy
1 2 3 4 6 1 K 9 10 11 12
5Control 16 17 13 16 12 4 4 16 15 16 6 13
7 16 IX 15 14 16 20 24 16 18 15 100 29.8 Not applicable211710.1
32 35 28 30 28 2824 32 33 31 27 30
90%16 16 15 17 5 18 14 14 17 5 5 18
7 16 17 14 26 15 14 17 15 1002120 30.4 9.316 -2.0
Total 32 33 29 31 2833 31 32 26 25 34
I'urhnmJ^ l'> IQ
Total
5
Chronic Whole Eflhient Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia
Quality Control
Verification of Data Entry, Calculations, and Statistical Analyses
Average
rcproduct ion
(ofTspriiig/rcin«dc)
Dtniiictl’s MSD value:
I’MSI):
USTPA 2000. I 'nderstaiiiliiig and Accounting for Method Variability in Whole Effluent Toxicity Applications Under die National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection
Agency, Cincinnati. (311
Ccmcentriition
(%)
Survival(%)vntiiilioii
(%)
2.998
10.0
Ntiniber of young produced by replicate number
5
Coelllricnl of I’crcent reduction from
control (%)
Client: CET - Durham County - Triangle WWI P
Test dates: May 19 - 26, 2004
Project number: 1260
MSD = Minimum Significant Difference
PMSD = Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is die minimum percent difference between the control and treatment that can he declared
statistically significant in a whole effluent toxicity lest. On average, a significant difference occurs for Environmental Testing Solutions. Inc.
chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 10.2% from die control.
Lower PMSD bound determined by USEPA (10U1 percentile) =11%.
Upper PMSD bound determined by USEPA (90lh percentile) = 37%.
The lower and upper bounds were calculated by die USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in
chronic reference toxicant tests.
14 ___
31
1
Laboratory Benchsheets
and Statistical Analyses
Environmental Testing Solutions. Inc.Page 1 of 1
Project #
5 0^
4 % Adults having 3ra Broods (> 80°/o):(009,
3 % Mortality (< 20%):
2
Date Analyst
0
1 Feeding
2 Renewal I o$-is-o4
3 Feeding
4 Feeding
5 Renewal 2 oS-\S-o4
6 Feeding
7 Termination
Off 55
I 2 3 4 5 7 8 129
Adult mortality c.L_L__<_L.L_V
Young produced5 4 4 is5 10
Adult mortality v_L__1—L.L I—L
6 or 7 Young produced /O'/q‘5 ‘5 i5 15
Total young produced 2^2^>3 I 352050
Final Adult Mortality L_L-L_L L.0_L_
Note: An X in the upper right comer of the number of young produced indicates the presence of two broods.
0?O5
< 24-hours old__________________
OS-lfc-O'A 0^1-5 TO IZC^
Q-IS-g.
2.^
Final
~ZS. \
Final
2^2-
Final
•^4. <S
Initial
Test organism information:
Organism age;_______________
Date and times organisms
were bom between:___________
Organism source:_____________
Transfer bowl information:
Daily renewal information:
Day
Acceptance Criteria:
% of Male Adults (< 20%):
Test information:
Randomizing template:
Incubator number and
shelf location:
YCT batch:
Selenastnim batch:
ABS
ABS
~7 ~75
S~-o
33
Initial
oeqz_
056^
Mean Offspring/Female
(> 15 offspring/surviving female):
% CV (< 40.0%):
Control water batch used
(SSW)
0645-04
Day
2
Initiation
1-7fa
7-6
24-^
Initial
Survival and Reproduction Data
__________Replicate number__
6
09-16-0^
0^20-0^
t>9-Zl-o4
ce>'2.-i.cA
oe>.z4.c4
oS-zs.oI
Renewal Two
Test grouping information:
6
10 11
L
02_
24.3
9.3-7.
7.7k
7.9
1S5
22^
174
________Parameter_____
Analyst
pH(S.U.)_______________
DO (mg/L)______________
Conductivity (pmhos/cm)
Alkalinity (mg CaC03/L)
Hardness (mg CaC03/L)
Temperature (°C)
Renewal One
-7-^
2\k
Control Information, North Carolina Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013
Method 1002.0, NC Modification- February, 1998) Species: Ceriodaphnia dubia
Control # I Date
—
CAT
I6e2
-4
Test initiation, renewal,
feeding, or termination time
Initiation . _n5 z
or-to-oq A-p____________
pH =&.'L5SU Temperature= 2 5.2° C
15*
X for 3rd Broods
Noctfr Ccvi^uXaJJZ
Cfqy err Co uCcoJ
PjcJnar-TA Co-______
1 CONTROL
Hx-13*
Page 1 of 1Environmental Testing Solutions, Inc.
I Date Parted with Control #
Comments:
Sample information:
43^
zo.le><0-10
Test Concentration:
1210119845321
Adult mortality u LkuL_L k—
345Young produced 3 35333
C.L_Adult mortality v_L LLCk__
15Young produced6 or 7
2.^2~l50Total young produced 35 2_(o Z_71ZLCo32-31
L_k_L_Final Adult Mortality Lt—LL_L L
Vote: An X in the upper right comer of the number of young produced indicates the presence of two broods.
Client:
Facility:
Pals Seccoud
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test
(EPA-821-R-02-013 Method 1002.0, NC Modification- February, 1998)
Species: Ceriodaphnia dubia
3^2.1. O'
Full-strength chemistry:
Parameter
Test concentration chemistry:
Parameter
Collection start date:
Collection end time:
Grab/Composite
(duration):___________
Temperature (°C) upon
receipt: __________
Physical characteristics:
ETS project number:
ETS sample number:
Test results:___________
% Mortality:___________
Mean Offspring/Female:
% Reduction from control
Calculated t;___________
Tabular t:______________
Pass or Fail
CC\
2^TO
30
05
pH (S.U.)
DO (mg/L)
Conductivity
(pmhos/cm)
Chlorine
(mg/L)
Dilution preparation:
Test concentration:
mL Sample:
mL Dilution water:
Day
2
Analyst_______________
pH (S.U.)_____________
DO (mg/L)____________
Conductivity (umhos/cm)
Temperature (°C)
NPDES#: NC
______________County:______
U- Uju3TP | Qutfall/Pipe: ~
Survival and Reproduction Data
________Replicate number
7
Sample 2
r>aywl
o^lg
zi
CDr-y9os.bt
(■2 -c
CO
Sample 2
5-1____
Initiation
PCB
J.M
zq.S
Final
6
L-
Sample 1
Qg-Uo-CM
103
25.1
Final
Sample 1
6
-~h~l 1
5*1
-z.q.q
Initial
Renewal Two
~l-7
~7.qu
Final
(jP/T________________ rro dp .-fcOeo
"M lb. ~
Renewal One
PCS
1.^
17 z.
zs. I
Initial
3/70
0.Z.
40^ zs-5
Initial
2^2^2^^
i4z
30.000 29.00032.000 26.000 28.000 33.000 26.000 27.000
MSDt-Stat
0.405 2.508 2.579
Dose-Response Plot
40
35
30
o 25 ■■
20 ■
10 -
5 ■
g
Durham_08-l 8-04
8/18/2004
8/25/2004
25.000
26.000
CET - Durham County - Triangle WWTP
DMR-Discharge Monitoring Report
CD-Ceriodaphnia dubia
1-Tailed
Critical
1-tail, 0.01 level
' ’ of significance
Environmental Testing Solutions, Inc.
Statistical Analyses
Test ID:
Lab ID:
Protocol:
5_____
25.000
Auxiliary Tests_________________________________
Shapiro-Wilk's Test indicates normal distribution (p > 0.01)
P-Test indicates equal variances (p = 0.55)_____________
Hypothesis Test (1-tail, 0.01)_______________________
Homoscedastic t Test indicates no significant differences
Treatments ss D-Control
Start Date:
End Date:
Sample Date:
Comments:
Gonc-%
D-Control
D-Control
90
90
Conc-%
D-Control
90
2_____
29.000
30.000
29.000
29.000
N
12
12
8____
26.000
1____
26.000
33.000
31.000
27.000
2___
30.000
4_____
30.000
6_____
31.000
7____
28.000
9_____
34.000
10____
30.000
df
1,22
N-Mean
10000
0.9858
Mean
29.333
28.917
MSDu MSDp MSB________MSB F-Prob
2.57936949 0.08793305 1.04166667 6.34469697 0.68925089
Statistic
0.96866727
1.45241582
Critical
0.884
5.31966734
Skew Kurt
0.13364172 -0.6336926
Mean
29.333
28.917
0 ■ -
Ceriodaphnia Survival and Reproduction Test-Reproduction
CDPFCRNC Sample ID:
ETS-Environ. Testing Sol. Sample Type:
CHRONIC-EPA-821-R-02-0I3 Test Species:
Transform: Un transformed
Min Max CV%
34.000 9.346
33.000 7.866
o
o
•a
a.
£ ’5;
Environmental Testing Solutions, Inc.
Reveiwed by:
Day
Number of young produced by replicate number
I 2 3 4 5 6 7 8 9 10 11 12
5Control 4 4 5 18
4 4 14 13 19 15 16 16
7 22 25 26 25 13 100 29.3 9.3 Not applicable21141315151714
Total 26 29 30 30 25 31 28 26 34 30 33 30
590%4 4 3 3 3 5 4 3 14 3 3 3
7 27 25 100 28.9 7.9 1.42923 25 28 22 27 15 24 24 26
Total 31 29 32 28 2626 33 30 29 27 27 29
l)iirhiii>i_OS /A' I1-/
USEPA. 2000. Understanding and Accounting for Mediod Variability in Whole Effluent Toxicity Applications Under tlie National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection
Agency, Cincinnati, OU.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia
Quality Control
Verification of Data Entry, Calculations, and Statistical Analyses
Average
reproduction
(olTspring/femnle)
Percent reduction from
control (%)
Dunnell's MSI) value:
PM SO:
Survivtil
(%)
Concentration
(%)
2.579
8.8
CoelTicient of
variation
(%)
Client: CET - Durham County - Triangle WWl’P
Test dates: August 18-25, 2004
Project number: 1413
MSD = Minimum Significant Difference
PMSD = Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between die control and treatment dial can be declared
statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc
chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 10.1% from die control.
Lower PMSD bound determined by USEPA (IO01 percentile) =11%.
Upper PMSD bound determined by USEPA (90lh percentile) = 37%.
The lower and upper bounds were calculated by die USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in
chronic reference toxicant tests.
Reference Toxicant Control Charts
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements
CTTest date PMSDCTCVCTMSD
for PMSD (%)(%)(%)(%)(offspring/female)
Note:
08-10-04
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic
reference toxicant tests.
USEPA 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge
Elimination Program EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Test
number
01-07-03
02-04-03
03-05-03
04-11-03
05-06-03
06-04-03
07-08-03
08-05-03
09-10-03
10-07-03
11 -04-03
12-04-03
01-06-04
02-03-04
03-03-04
04-06-04
05-04-04
06-08-04
07-07-04
08-10-04
Control
Survival
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
Sodium Chloride Chronic Reference Toxicant Data
for Ceriodaphnia dubia
using Moderately Hard Synthetic Water
Control Mean
Reproduction
for Control Mean
Reproduction
(offspring/female)
32.8
31.4
30.1
29.6
29.0
29.0
29.5
29.5
29.9
30.0
30.0
30.1
30.1
30.0
29.9
30.1
30.2
30.4
30.2
7.5
6.7
6.6
7.4
7.2
7.8
7.7
7.3
7.4
7.4
7.5
7.3
7.3
7.3
7.3
7.2
7.2
7.1
7.1
8.5
9.7
9.7
10.0
10.0
10.2
10.7
10.7
10.5
10.3
10.5
10.5
10.4
10.4
10.4
10.4
10.3
10.2
10.1
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
33.2
32.3
28.7
26.3
27.6
25.9
29.0
33.3
29.3
33.4
31.0
30.4
30.6
30.7
29.0
28.1
32.5
32.9
33.3
27.4
7.0
8.1
5.1
6.2
10.8
5.9
11.6
6.6
4.3
8.0
7.3
9.7
4.4
7.2
7.8
6.2
6.4
6.8
6.3
5.8
2.9
2.7
3.5
2.5
3.2
2.6
3.2
4.7
3.1
3.2
2.6
3.6
3.5
2.7
3.0
2.8
3.3
3.0
2.6
2.3
8.6
8.4
12.1
9.6
11.5
10.1
10.9
14.1
10.7
9.5
8.3
11.8
11.5
8.8
10.3
10.0
10.1
9.0
7.8
8.6
for Control
Reproduction
CV (%)
CV = Coefficient of variation for control reproduction.
On average, the CV for control reproduction is 7.1 % in Environmental Testing Solutions, Inc. Ceriodaphnia chronic toxicity
tests.
Lower CV bound determined by USEPA (IO111 percentile) = 8.9%.
Upper CV bound determined by USEPA (PO01 percentile) = 42%
MSD = Minimum Significant Difference
PMSD = Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can
be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for
Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 10.1%
from the control.
Lower PMSD bound determined by USEPA (10* percentile) = 11%.
Upper PMSD bound determined by USEPA (90* percentile) = 37%.
CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD)
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart
Sa.io Sa.25Test number Test date S
cr-s,V91lCT + S^s CT + Sa.,5CT-S^o
Note
IJSEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency. (Cincinnati. Ot 1
01-07-03
02-04-03
03-05-03
04-08-03
05-06-03
06-04-03
07-08-03
08-05-03
09-10-03
10- 07-03
11- 04-03
12- 04-03
01-06-04
02-03-04
03-03-04
04-06-04
05-04-04
06-08-04
07-07-04
08-10-04
0.96
0.99
1.05
1.03
1.05
1.07
1.03
1.04
1.05
1.02
1.08
1.08
1.06
1.06
1.07
1.07
1.07
1.06
1.06
1.06
CT
(g/L NaCl)
0.98
1.00
1.01
1.02
1.02
1.02
1.03
1.03
1.03
1.03
1.04
1.04
1.04
1.04
1.04
1.05
1.05
1.05
1.05
0.02
0.05
0.04
0.04
0.04
0.04
0.03
0.03
0.03
0.03
0.04
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.94
0.91
0.93
0.94
0.94
0.95
0.96
0.96
0.96
0.96
0.97
0.97
0.97
0.98
0.98
0.98
0.98
0.98
0.99
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
for Ceriodaphnia dubia
using Moderately Hard Synthetic Water
0.90
0.92
0.93
0.93
0.94
0.94
0.94
0.95
0.95
0.95
0.95
0.96
0.96
0.96
0.96
0.96
0.96
0.96
0.96
1.05
1.08
1.09
1.10
1.11
1.11
1.11
1.11
1.11
1.12
1.12
1.12
1.12
1.13
1.13
1.13
1.13
1.13
1.13
0.17
0.17
0.17
0.17
0.17
0.17
0.17
0.17
0.17
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.81
0.83
0.84
0.84
0.85
0.85
0.85
0.85
0.85
0.86
0.86
0.86
0.86
0.87
0.87
0.87
0.87
0.87
0.87
0.44
0.45
0.45
0.46
0.46
0.46
0.46
0.46
0.46
0.46
0.47
0.47
0.47
0.47
0.47
0.47
0.47
0.47
0.47
0.54
0.55
0.55
0.56
0.56
0.56
0.56
0.57
0.57
0.57
0.57
0.57
0.57
0.57
0.57
0.58
0.58
0.58
0.58
0.37
0.38
0.38
0.39
0.39
0.39
0.39
0.39
0.39
0.39
0.39
0.39
0.40
0.40
0.40
0.40
0.40
0.40
0.40
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
1.01
1.09
1.09
1.09
1.11
1.10
1.10
1.10
1.09
1.10
1.11
1.11
1.11
1.11
1.11
1.11
1.11
1.11
1.11
1.14
1.17
1.18
1.19
1.20
1.20
1.20
1.20
1.20
1.21
1.21
1.21
1.22
1.22
1.22
1.22
1.22
1.22
1.23
1.41
1.45
1.46
1.47
1.48
1.49
1.49
1.49
1.49
1.50
1.50
1.51
1.51
1.51
1.51
1.52
1.52
1.52
1.52
0.02
0.05
0.04
0.04
0.04
0.04
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
7-<lay IC15
(g/L NaCl)
0.60
0.62
0.62
0.63
0.63
0.64
0.64
0.64
0.64
0.64
0.64
0.64
0.64
0.65
0.65
0.65
0.65
0.65
0.65
1.58
I 62
1.63
1.64
I 66
1.66
1.66
1.67
1.67
1.67
1.68
1.68
1.68
1.69
1.69
1.69
1.69
1.70
1 70
Sa.7$
State and USEPA
Control Limits
CT-2S CT + 2S
Laboratory
Warning Units
CT + S^jo
USEPA
Wanting Units
CT - S,V75
Laboratory
Control Linits
CT-S^s
USEPA
Control Units
CT + S^9I,
7-d IC25
CT = Central tendency (mean IC2S).
S = Standard deviation of the IC25 values.
Laboratory Control and Wanting Units
Laboratory control and wanting limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25tli percentile CVs. These ranges are more stringent than the control and wanting
limits recommended by USEPA for the test method and endpoint
Sa.10 = Standard deviation corresponding to the 10lh percentile CV. (S^^ = 0.08)
Sa_25 = Standard deviation corresponding to the 25"' percentile CV. (SA25 =0.17)
USEPA Control and Warning Linits
Sa.7s = Standard deviation corresponding to the 75a' percentile CV. (SA7S = 0.45)
SA90 = Standard deviation corresponding to the 90"' percentile CV. (Sa.<)o = 0.62)
CV = Coefficient of variation of the IC2S values.
= 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Environmental Testing Solutions, Inc.
1.20
1.15
1.10
1.05
1.00
0.95
1.5 T TTT
1.4
1.3
1.2
1.1
1.0
0.9
0.8
Test date
Sodium Chloride Chronic Reference Toxicant Control Chart
for Ceriodaphnia dubia
using Moderately Hard Synthetic Water
Laboratory Warning and Control Limits
Limits Set According to 10th and 25th Percentile CVs
U cs
Z
IT, n u
05
■
A.io)
or 2 Standard Deviations)
>->5
7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride
that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25 ± S
Control Limits (mean IC25 ± SA25
I I
■»
State and USEPA Mandated Control Limits
Limits Set According to ±2 Standard Deviations
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 11/11/04
Facility.- TRIANGLE WWTP County: DURHAM
INC .
Comments:
Work Order:
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
% Mortality Avg.Reprod.# Young Produced 23 20 21 23 26 28 26 17 20 24 21 26
Adult (L)ive (D)ead L L L L L L L L L L L L
1 2 3 4 5 6 7 8 9 10 11 12
PASS FAIL
# Young Produced 28 24 27 26 24 26 18 31 16 27 25 29 X
Check OneAdult (L)ive (D)ead L L L L L L L L L L L L
1st sample 1st sample 2nd sample
PH
Control 7.97 8.08 8.01 7.92 7.99 8.01
Sample 2: 11/04/04Treatment 2 7.65 7.73 7.68 7.67 7.36 7.79
1st
Grab Comp.Duration
Sample 1 24 hrsX
Sample 2 X 24 hrs
Hardness(mg/1)447.84 7.66 7.97 7.41 7.81 7.78
Spec. Cond.(gmhos)180 584 64 5Treatment 2 7.32 8.34 7.18 7.48 7.72
Chlorine(mg/1)<0.1 <0 .1
LC50/Acute Toxicity Test Sample temp, at receipt(°C)0.3 0.1
combining replicates)
%%%%%%%%%%Concentration
%%%%%%%%% %
start/end
Control
PH D.O.
Organism Tested:Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT-1 (3/87)4.41)rev.
7.59 ____
High
Cone .
Method of Determination
Moving Average
Spearman Karber
Probit
Other
Complete This For Either Test
Test Start Date: 11/03/04
Collection (Start) Date
Sample 1: 11/01/04
Sample Type/Duration
Chronic Test Results
Calculated t =
Tabular t =
% Reduction =
D
I
L
U
T
S
A
M
P
S
A
M
P
s
t
a
e
n
d
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Control CV
14.104%
0.00
Control
22.92
Control
2nd
P/F
e
n
d
e
n
d
0.00
Treatment 2
25.08
Treatment 2
LC5 0 = %
95% Confidence Limits
% %
-1.396
2.508
-9.45
Mortality
start/end
D.O.
Control
s
t
a
r
t
1st sample
t
1st sample
Effluent %: 90%
TREATMENT 2 ORGANISMS
s
t
a
r
t
2nd sample
Note: Please
Complete This
Section Also
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
11/95 (DUBIA ver.
% control orgs
producing 3rd
brood
100%
-9.45% Reduction *
(Mortality expressed as %,
* PASSED;
NPDES#: NC0026501 Pipe#:
Laboratory Performing Test: MERITECH LABS,
Signature of Opferator in Responsible Charge
x jL__’v—__________________________
~ Signature of Laboratory Supervisor
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State: //<
Sample Information
a:
TpcrrmpS?
Toxicity Test Information
Sample Receiving (Laboratory Use Only)
a:
Test Required: Chronic:
Acute:
Test Organism: Ceriodaphnia Dubia: y
Pimephales promelas:
Mysidopsis bahia:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
TimeOr-5^
# of Containers 2
: oZBO fAWPM
Date:, (1. bf'
Sample Condition:
Sampling Site: p{f Cyi.))dP^I! ~ Of J) 0^ OA
Sample Type:
Sampling Time:
______ PO#_____________
____ NPDES #: A/C/^-oOl
_____ Phone:
____ Pipe Number:
Zip: ,^77/5
Type of Facility Generating Effluent: Tpironnk IrfG^r}!: hlf/lfnl
START
END
Sample Chilled {Covered with Ice): Yes -X
Collector’s Name {Print): Nif / A - O'lJrv^CCi//
Collector’s Signature: Q-
Client Information
Client: f Curran
Contact: Bahl'k
Address: fdC /AvAi PP /BjpJ:
City: fiprharn
County: Jfyrhai!!
0C
IWC: 7
Toxicity Test Concentration(s): A)} lUuQCipy Q()/.
List Any Special Requirements: ho')l C/iWC
Shipping Information
Relinquished By: Q •
Received By:
Relinquished By:
Received By:
Sample Temperature (C°):
Date: /Jp/pd Time: /^Qf) AM (@)
Date: Time: PM
Date: Time:AM PM
Date: Time:AM PM
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
Grab / . Composite
Date: iih/fply flV/i'gZ)Time:
Date: // /p ppifl ime:
r' ' xz” ‘ s No
Received From: {^~\C C-O pv-.
Received By: 'TM \_tJ<.vAC>________
Sample Temperature (C°): 0-3 / C-3
r /'fr iliLx/
Toxicity Test Information
Test Required: Chronic:j.
Acute:
START
END
Relinquished
Received By:
Relinquished By:
Received By:
Sample Temperature (C°):
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
Time: 7?/6^AM PM
Time: / f fp AM PM
Time:AM PM
Time: AM PM
Client Information
Client: r Currort_____________
Contact: "hmonf /jW/Zc _______
Address: atyh NC if JU. CT _______
City: /W/aA)______J ____________
County: State: /VC
Type of Facility Generating Effluent: ^riarC/K
______ PC#_____________
____ NPDES #:
_____ Phone: Cqiq) CW-fbf /
___ Pipe Number:
Zip: &7 7/')________
# of Containers f
Time: PM
Time: pfa/T @pPM
No
Sample Receiving (Laboratory Use Only)
R e c e i v e d Fro m: ______
Received By: Date: PM Time: cj~7) oAMCfTTA
Sample Temperature (CQ): O-l / O1 Sample Condition: 4-C G—-CD
IWC: . ,
Toxicity Test Concentration(s): Di/uliw - fQf
List Any Special Requirements: Ppqq/ dpi/ GfaniC

Test Organism: Ceriodaphnia Dubia: ;/
Pimephales promelas:
Mysidopsis bahia:
Sample Information. . (/
Sampling Site:. QuLloJl’ (ydHOG
Sample Type: Grab |/ Composite
Sampling Time: START Date: ijlflpf
END Date: //AtW
Sample Chilled (^Covered with Ice)'. Yes 1/
Collector’s Name {Print)'. - (9A/AcrjL
Collector’s Signature: ^2/z/f}-
Shipping Information
Date:
Date: j '/ -
Date:
Date:
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date.11/11/2004
Facility Triangle Wastewater Treatment Facility NPDES # NC0026501 County Durham
MAIL ORIGINAL TO:
Test initiation Date/Time 11/2/2004 /2 45 PM Avg Wt/Surv. Control [0.4381 Test Organisms
% Survival f 100 01
Avg Wt (mg) [0.438 |Hatch Date:11/1/04
% Survival [100 01 Hatch Time: i.oo-s oopm
0 431
100 ~
0.372 |
L
0.433 |Avg Wt (mg) [
% Survival [100 0 I
Avg Wt (mg) [0 454 I
L 100.0 I% Survival [
Avg Wt (mg) f 0 432 |
DWQ Form AT-5 (1/04)
1
11/1/2004
100.0 I
Avg Wt (mg) [
% Survival [
Avg Wt (mg) [
% Survival F
Cultured In-House
Outside Supplier
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Dilution H2O
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlonne(mg/L)
Temp at Receipt (°C)
22 5| Surviving #
Original #
Wt/original (mg)
1001 Suiviving #
Original #
Wt/original (mg)
901 Surviving tf
Original #
Wt/origmal (mg)
751 Surviving ft
Original #
Wt/original (mg)
451 Surviving #
Original #
Wt/original (mg)
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Normal
Hom. Var.
NOEC
LOEC
ChV
Method
Pipe #- 001
Comments |
7 97
7 76
25 0
41.00
67 00
248
10
10
0.451
10
10
0.409
10
10
0.413
10
10
0.333
10
10
0.448
1
10
10
0.494
7 06
7.95
25.0
8.00
7 70
25.0
2
11/2/2004
10
10
0.528
10
10
0 524
10
10
0.413
10
10
0.398
2
10
10
0 379
7.64
8.25
25.0
3
11/4/2004
10
10
0.392
10
10
0.450
10
10
0.448
10
10
0.386
10
10
0 400
3
10
10
0 432
8.17
7 06
24.9
7 66
8.20
25.0
8.10
7.83
25.0
10
10
0 433
10
10
0 457
10
10
0.371
4
10
10
0.446
Stats
Cone.
22.5
45
75
90
100
7 39
8.13
25.0
8.05
7 78
25.0
Survival
Critical
10
10
10
10
10
7.40
8.10
25 0
8.03
7,80
25 0
Critical
2.41
241
2.41
241
2.41
7.50
7.46
24 7
% Eff. Repl.
[Control | Surviving it
Original #
Wt/original (mg)
7 04
8 02
25 0
10
10
0.419
24,00
96 00
30 00
643
<0 1
0 1
10
10
0.355
10
10
0.458
Calculated
18
18
18
16
18
8.08
7.41
24 9
24.00
78.00
22.00
586
<0 1
0.3
24,00
80.00
37 00
657
<0.1
0 5
1_____
/ 7,99
/ 7.51
I 24.8
4______
~/ 8 09
I 7,45
/ 24.8
0
/ 7 69
/ 750
I 24 8
1
7 7 79
/ 7.61
I 24.7
Laboratory Mentech. Inc.
X /
Signature ofjOperator in Responsible Charge
x -______
Signature of Laboratory Supervisor
Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
0
/ 8 00
/ 7 38
/ 24.9
5______
/ 8.06
/ 7 40
I 24.9
4
7 41
/ 7 53
/ 24.7
5______
/ 7.46
/ 7 50
/ 24 8
3
/ 7 76
/ 7,11
/ 24.8
______6
7 43 /
8 08 /
25.0 /
______2
8.13 /
7,80 /
25.0 /
6
803 /
783 /
25.0 /
2
I 7.71
/ 7.05
/ 24.7
Growth
Fl
Fl
100
>100
>100
Dunnell’s
Survival
Fl
Fl
100
>100
>100
Steel’s
Growth
Calculated
0 2019
2 0421
0.1553
-0 5047
0 1941
Overall Result
ChV |
Day
3
7 8.14
/ 7.10
/ 24.9
Initials Date Time 1 Initials Initials Time 3Day 1 Initials
Day 0 5 L(0oV—TODay 2
Day 1
C»,L>p Le'uo r S.Day 3 11-s' cy s\Day 2 Jr4-<Af S'<!-< eoK. Day 4 I l-U -cv
Day 3 /(■‘’r-O'y Wa---St, ^oog j'Day 5 1- ls/ifh JLlk1!Day 4 / (-U'CTf llooViM
Day 6 Ltfcev ^30/
Day 5
n-0'04

I
‘!lOr-
Feeding
Time 2
« of Organisms per Chamber: )O
--------ize:
J? $2'; 71 £
Day 6
—•
MERITECH
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Dilution Water:
Randomization:
Incubator #:
i est Initiation Data:
Start Date: M
Start Time: —
I n 111a Is/S igna tu re: .51. X.
Date/Time Fed ; v-TZmIHEZZZ
Date/Time BornjFn^
S
Test Vessel Size:
Test Solution Volume:__
Temp, of Stock:
tt of Reps:
Test Termination Data-
End Time: -
Transfer
Date Time
Facility CUl^ rP
NPDES
NO

RemarksJ
I
2s s
P.O.
Res.
Remarks31
Temp.
or
pH
^7
Remarks • c532
J.c.
I
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1
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Initial
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Initial
Final
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Conduct.Initial
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NPDES Test Dates:
it- <. Ana 1 y s tSL Test Time:
Chemical and Physical Determinations
Cone. LnhX I___
Temp. Initial
Final
J m ti a I
Final
Furnnf
Final
Alkalinity
Hardness.
Conduct. Initial XHA'
Final
Chlorine
/OF
CONG REP.# ORG.SURV %
A
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FOIL WT.
(mg)
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ORG. (mg)
WT. OF
ORG. (mg)
MEAN WT/
ORG. (mg)
r
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MER1TECH, INC.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
. U'
W3!. 3 6
PAGE/
OUTFALL:_
INITIALS:
FACILITY:
NPDES #:
DATE OF TEST: Z|-Z-^
DATE WEIGHED: )|- lfa "op-
ORGANISM: L?c
CONG REP.# ORG.SURV %
A
loo& <434 a |o
B
[V 0-4/s | coy,/3
c 9-0 lo4^^O- 443
D
ICO1°i-4(M.sq-o-q^
A
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B
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FACILITY:
NPDES#:'
DATE OF TEST:_
DATE WEIGHED:
ORGANISM:
FOIL WT.
(mg)
WT. OF
ORG. (mg)
FOIL WT. +
ORG. (mg)
MEAN WT/
ORG. (mg)
Pc
MEPJTECH, INC.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Espy
^o.^o
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iVC1 __________
A-A-oy
H-1 o -e~i
PAGE <2 0F
OUTFALL:
INITIALS:
B <-f 3-
r
Concentration CrtrfYef
Day 7Day 6DayDay 3Day 2Day 1Day 0
oRep #1
c/Rep #2
Rep #3
oooRep #4
Concentration^
Day 7Day 6Day 5Day 4Day 2Day 1Day 0
oRep #1
'OuRep #2
Rep #3
o oRep #4
Day 7Day 6Day 4Day 2Day 1Day 0
cZlRep #1
oooRep #2
Rep #3
b oORep #4
Day 3
o
Day 3
D
Day 5
o
Day 5
^.5
o
MERITECH
Mortality Data: Chronic Fathead Test
Start Date/Time End Date/Time
Concentration__9X_
r
Concentration
Day 7Day 6Day 5Day 4Day 3Day 2Day 1Day 0
o Q OORep #1
o oo oG nRep #2
o O o o Rep #3
OORep #4

CjdConcentration
Day 7Day 6Day 5Day 4Day 3Day 2Day 1Day 0
oo o Rep #1
o o■o Rep #2
O oooRep #3
o Rep #4
/OPConcentration
Day 7Day 6Day 5Day 4Day 3Day 2Day 1Day 0
o Rep #1
u Rep #2
JC
CC C ) Rep #3
O oRep #4 r
MERITECH
Mortality Data: Chronic Fathead Test
Start Date/Time <;c//GiG End Date/Time
State: //<
A.
Shipping Information
Sample Receiving (Laboratory’ Use Only)
Relinquished By:
Received By:
Relinquished By:
Received By:
Sample Temperature (C ):
Test Required: Chronic:
Acute:
START
END
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
Client Information
Client: ^/onfarj r Curran
Contact: ' /fM.
Address: /dC Hwy SC
City: ftyrharr}__________
County: [fychan________
Date: (1 l<2- I bf Timer^C^
Sample Condition: 41
______ Time: /sjff) AM ^M)
Date: q- Time: />A<fAAM PM
Date: Time:AM PM
Date: Time:AM PM
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
IWC:______________ /;
Toxicity Test Concentration(s): 9.0/^:
List Any Special Requirements: Pq^sIhiiI ClrOfiiC
Date:
I —
lie.
Received From: |'MQ.
Received By: Luc vAcZ
Sample Temperature (C ): 0-3 / 0-3
______ PO#_____________
____ NPDES #: /vCtfblCoOl
_____ Phone: A7/-7OW- ^,F/
___ Pipe Number:
Zip: ^77/5_______
Type of Facility Generating Effluent: 'fyiongk irfupYBf' hlu/'h
Sample Information
Sampl ing Site: a/7/z,*7o/ OiiHall- pf / / ()f< 0^.___________________________
Sample Type: Grab y/ Composite # of Containers
Sampling Time: START Date: op p. i me: Q fCC ^AVPM
END Date: //^ /^y/y^Time: QftdO CAW PM
Sample Chilled {Covered with key. Yes 1/ No
Collector’s Name {Printy /fh! A■O'Drhcci/! ______________
Collector’s Signature: CZzxZL (7
Toxicity Test Information
1/0'3
Type of Facility Generating Effluent:
Collector’s Signature:
Test Required: Chronic:
Acute:
Test Organism: Ceriodaphnia Dubia: [/
Pimephales promelas:
Mysidopsis bahia:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
# of Containers J
Time: AM
Time: AM
No
______ PO#______________
____ NPDES #: /Vrp)C^-oO/
_____ Phone: (y/c?) vp/. /fpp/
___ Pipe Number:
Zip:
Client Information
Client: f Cyrran______________
Contact: irone',
Address: . jy fat
City: Dnmarn, J
County: 'fa fan State: /ff
Time: SV' AN^Jmj
_iCAs ___
Time: AZL AmZm
Time: /J ■ ?) A^fPNT
Time:AM PM
Time: AM PM
IWC:__________
Toxicity Test Concentration(s): f)fa jar- 90%
List Any Special Requirements: ft-fa/ fa! Qfanic
Sample Receiving (Laboratory Use Only)
Received From:
Received By: Date: IJ^>1 O H
Sample Temperature (C ): Q.^C!CB^C^ Sample Condition:
Shipping Information
Relinquished By: 'faftCft faj/y/fa Date: f/ks/of
Received By: Date:
Relinquished By: Date:
Received By:_____________ Date:
Sample Temperature (Cu): Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
Sample Information.
Sampling Site: ft/fflifa OMoll- Of _____
Sample Type: Grab \/ Composite
Sampling Time: START Date: //faof
END Date: i / ft jo f
Sample Chilled {Covered with Ice}: Yes Z /
Collector’s Name (Print): ^/f,/ ft. Qftfar/I
Collector’s Signature:
Toxicity Test Information
•fMB.
Toxicity Test Information
Received By:
Test Required: Chronic: </
Acute:
START
END
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
Time: PM
Time: / SCg AM PM
Time:AM PM
Time: AM PM
Client Information
Client: > Curran_______________________ PO H____________
Contact: fmorf &///< NPDES /i/rutVS /
Address: /\/C /dk/U, Phone: Wd-ftf /
City: finrhw J Pipe Number:
County: /f-cha/r} State: /j,/C Zip: 7/o
Type of Facility Generating Effluent: 'f'rianok fCcil/Lf
IWC _____________
Toxicity Test Concentration(s): Dilijlian -
List Any Special Requirements: /hli
ML__
/ Umdc
________ v x Date: Time: 3-^3AM(pM\.
Sample Temperature (CQ): O-l / O~ Sample Condition:
# of Containers R?
Time: pfdC_____fAM? PM
Time: (CKpPM
No
Sample Receiving (Laboratory Use Only)
Received From:< O\XV-\:D<3
Sample Information . .
Sampling Site:. FJthlM: QLL/C ry-! it Gb 0^_____
Sample Type:Grab </ Composite
Sampling Time: START Date: iily In f
END Date: itjLjcF
Sample Chilled {Covered with Ice\. Yes pA
Collector’s Name (PrinCy. fGj ! L ffJ)n^calC
Collector’s Signature: LlJ J Jr Ch- OwLxfhllr
____________________ /__________\________
S h i p p i n g I n fo rm a ti o n
Relinquished By/) CjyiLlCLyi\
Received By:
Relinquished By:
Received By:
Sample Temperature (C°):
Date: /2 -
Date: // -
_ Date:__________
_ Date:
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
Test Organism: Ceriodaplinia Dubia:y
Pimephales promelas:
Mysidopsis bahia:
NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
Data PASS normality test (alpha = 0.01).Continue analysis.
Triangle WWTP Chronic Fish 11/2/04
irifishll204 Transform:
0.0373
0.9782
= 0.8840 (alpha = 0.01
- 0.9160 (alpha = 0.05
Title:
File :
Critical W
W
, N = 24)
, N = 24)
D =
W =
)
no transformation
Bartletu's Test for Homogeneity of Variance
Calculated Bl statistic 5.6505 (p-value = 0.3417)
Data PASS Bl homogeneity test at 0.01 level.Continue analysis.
Critical B
Triangle WWTP Chronic Fish 11/2/04
Trifishll204 Transform:
(alpha = 0.01,
(alpha = 0.05,
5)
5)
Title:
File:
df =
df =
= 15.0863
= 11.0705
•/
NO transformation
ANOVA Table
SOURCE DF SS MS F
Between 5 0.0158 0.0032 1 - 5201
Within (Error)18 0.0373 0.0021
Total 23 0.0531
(p-value = 0.2330)
Critical F
Since F FAIL TO REJECT Ho: All equal (alpha = 0.05)
Title :
File:
4.2479
2.7729
(alpha = 0.01,
(alpha = 0.05,
df = 5,18)
df = 5,18)
Triangle WWTP Chronic Fish 11/2/04
irilishll204 Transform:
< Critical F
J
no transformation
Dunnett’s Test TABLE 1 OF 2 Ho:Control<Treatment
GROUP IDENTIFICATION T STAT
Dunnett critical value = 2.4100 (1 Tailed, alpha = 0.05,df 5, 18)
NO TRANSFORMATION
Dunnett's Test TABLE 2 OF 2 Ho:ControlcTreatment
GROUP IDENTIFICATION
i
Title:
File :
Triangle WWTP Chronic Fish 11/2/04
Trifishll204 Transform:
DIFFERENCE
FROM CONTROL
0.2019
2.0421
0.1553
-0.5047
0.1941
1
2
3
4
5
6
1
2
3
4
5
6
NUM OF
REPS
TRANSFORMED
MEAN
4
4
4
4
4
4
0.4378
0.4313
0.3720
0.4327
0.4540
0.4315
MIN SIG DIFF
(IN ORIG. UNITS)
0.0776
0.0776
0.0776
0.0776
0.0776
MEAN CALCULATED IN
ORIGINAL UNITS
0.4378
0.4313
0.3720
0.4327
0.4540
0.4315
% OF
CONTROL
17.7
17.7
17.7
17.7
17.7
0.0065
0.0657
0.0050
-0.0163
0.0062
Title:
File :
SIG
0 . 0E
Control
22.5%
45%
75%
90%
100%
Control
22.5%
45%
75%
90%
100%
Triangle WWTP Chronic Fish 11/2/04
Trifish.11204 Transform:
Reference Toxicant Control Charts
Environmental Testing Solutions, Inc.
Test nunibrr I cst (kite S s<23 ^A-TS ('VCT + 2S CT + S^,,CT+S,V75
Note reproduction for the test population.
stringent than the control and warning
CV =
a* 9/ gj
0.57
0.57
0.56
0.56
0.56
0.56
0.56
0.57
0.57
0.57
0.57
0.57
0.57
0.57
0.57
0.57
0.57
0.57
0.57
CT
(e/L NaCl)
1.03
1.04
1.02
1.01
1.02
1.02
1.02
1.03
1.03
1.03
1.03
1.03
1 03
1.04
1.04
1.04
1.04
1 04
1.04
0.00
0 01
0.04
0.03
0.04
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.0.3
0.03
1.03
1.02
0.94
0.94
0.95
0 96
0.96
0.96
0.97
0 97
0 97
0.97
0.97
0.97
0.98
0.98
0.98
0.98
0.98
1.04
1.05
1.09
1.08
1.09
1.08
1.09
1.09
1.09
1.09-
1.09
1.09
1.09
1.10
1.10
1.10
1.10
1.10
1.10
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.95
0.95
0.94
0.93
0.94
0.94
0.94
0.95
0.95
0.95
0.95
0.95
0.95
0.95
0.95
0.96
0.96
0.96
0.96
1.12
1.12
1.10
1.09
1.10
1.10
1.10
1.11
1.11
1.11
1.11
1.11
1.12
1.12
1.12
1.12
1.12
1.13
1.13
0.18
0.18
0.17
0.17
0.17
0.17
0.17
0.17
0.17
0.17
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.86
0.86
0.84
0.84
0.85
0.85
0.85
0.85
0.85
0.85
0.86
0.85
0.86
0.86
0.86
0.86
0.86
0.87
0.87
1.21
1.21
1.19
1.18
1.19
1.19
1.20
1.20
1.20
1.20
1.21
1.20
1.21
1.21
1.21
1.22
1.22
1.22
1.22
0.46
0.47
0.46
0.46
0.46
0.46
0.46
0.46
0.46
0.46
0.46
0.46
0.47
0.47
0.47
0,47
0.47
0.47
0.47
1.50
1.50
1.48
1.47
1 48
1.48
1.48
1 49
1.49
1.49
1.49
1.49
1.50
1.50
1.50
1.51
1.51
1.51
1.51
0.39
0.39
0.39
0.38
0.39
0.39
0.39
0.39
0.39
0.39
0.39
0.39
0.39
0.39
0.39
0.39
0.40
0.40
0.40
12-06-02
12-1 1-02
12-18-02
01-07-03
02-04-03
03-05-0.3
04-08-03
05-06-03
06-04-03
07-08-03
08-05-03
09-10-03
10-07-03
1 1-04-0.3
12-04-0.3
01-06-04
02-03-04
03-03-04
04-06-04
(15-04-04
7-duy IC„
(g/l.NaCl)
1.03
1 04
1.04
0.96
0.99
1.05
1.03
1.05
1.07
1.03
1.04
1.05
1.02
1.08
1.08
1.06
1.06
1.07
1.07
1.07
0.64
0.64
0.63
0.63
0.63
0.63
0.63
0.64
0.64
0.64
0.64
0.64
0.64
0.64
0.64
0.64
0.65
0.65
0.65
1
2
3
4
5
6
7
8
9
10
1 1
12
13
14
15
16
17
18
19
20
I 67
1.68
1.65
1.6-1
1.65
I 65
1.66
1.67
1.67
1.67
1.67
1.67
1.67
1.68
1.68
1.68
1.69
1.69
1.69
CT +
Sa.,0
USl-J’A 2000. Diulcrstaiiding and zXccoiinting for Method Variability in Whole EfTlucnl
These ranges are more
‘^A.10
-'a.io = Standard deviation
= Standard deviation
USEPA Control and Warning Idnits
Sx.,, = Standard deviation
sa.9d = Standard deviation corresponding tn the 90,h percentile CV. (S,
Cocllicicnl of variation of the 1C25 values.
State and USEPA
Control Units
CT - 2S
Sodium Chloride Chronic Reference Toxicant Control Chart
for Ceriodaphnia dubia
using Moderately Hard Synthetic Water
Laboratory
Warning Units
CT-S^
Laboratory
Control Units
CT-S^,
USEPA
Warning Units
CT-S^s CT - S.v,u
cause a 25% reduction in Ceriodaphnia
USEPA
Control Units
C3 +
4 Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-8.33-R-<)<)-(IO3. US Imvironiuc.l.l Pn.lectio.. Agency. Cincinnati. < >1
corresponding to the 75°' percentile CV. (SA7, = 0.45)
•a. 90 = 0.62)
0 1)0
0 01
0.04
0.03
0.03
0 03
0.03
0.0.3
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.0.3
7-d 1C15 ■ 7-day 25% inhibition concentration. An estimation of the concentration of sodium cliloride tliat would
< 1 = Central tendency (mean IC2)).
= Standard deviation (if the 1C2j values.
Laboratory Control and Warning Units
Laboratory control and warning limits were established using the sUndard deviation of the ICj, values corresponding to the 10th and 25th percentile CVs.
limits recommended by USEPA for the test method and endpoint.
corresponding to (he 10*1 percentile CV. (SA10 = 0.08)
corresponding to the 25^ percentile CV. (SA2J = 0.17)
Environmental J esting Solutions, Inc.
1.20
1.15
1.10
1.05
0.95
1.5 T T I T T
1.4
1.3
1.2 -
1.1
♦
♦
1.0
0.9
0.8 -
Test date
1.00
Sodium Chloride Chronic Reference Toxicant Control Chart
for Ceriodaphnia dubia
using Moderately Hard Synthetic Water
cs
Z
ir>
CMu
>>
-Q
i
A.10
or 2 Standard Deviations)
Laboratory Warning and Control Limits
Limits Set According to 10th and 25^ Percentile CVs
i
exh-
I I I I I
7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride
that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC,-)
Warning Limits (mean IC,5 ± S
Control Limits (mean IC?j ± SA -,5
i i
1------r
I I
■ xv"
I I
A
>>
State and USEPA Mandated Control Limits
Limits Set According to ± 2 Standard Deviations
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements
Test date CT CV CT MSD PMSD CT
(%)(offspring'female)(%)(%)for PMSD (%)
Note CV =
calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic
ONG-'-C'-l
1
o
USEPA 2000. Understanding and Accounting for Method Variability in WTiole EtUuenl Toxicity Applications Under the National Pollutant Discharge
Elimination Program. EPA-S33-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Test
number
Control
Survival
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
Sodium Chloride Chronic Reference Toxicant Data
for Ceriodaphnia dubia
using Moderately Hard Synthetic Water
Control Mean
Reproduction
for Control Mean
Reproduction
(offspring/female)
31.8
31.3
31.8
31.9
31.4
30.6
30.3
29.8
29.7
30.0
30.0
30.2
30.3
30.3
30.3
30.3
30.3
30.1
30.3
11.0
13.7
7.4
7.0
8.1
5.1
6.2
10.8
5.9
11.6
6.6
4.3
8.0
7.3
9.7
4.4
7.2
7.8
6.2
6.4
for Control
Reproduction
CV (%)
12.3
10.7
9.7
9.4
8.7
8.3
8.6
8.3
8.7
8.5
8.1
8.1
8.1
8.2
7.9
7.9
7.9
7.8
7.7
10.0
8.5
9.4
8.6
8.4
12.1
9.6
11.5
10.1
10.9
14.1
10.7
9.5
8.3
11.8
11.5
8.8
10.3
10.0
10.1
9.2
9.3
9.1
9.0
9.5
9.5
9.7
9.8
9.9
10.3
10.3
10.3
10.1
10.2
10.3
10.2
10.2
10.2
10.2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
12-06-02
12-11-02
12-18-02
01-07-03
02-04-03
03-05-03
04-11-03
05-06-03
06-04-03
07-08-03
08-05-03
09-10-03
10- 07-03
11- 04-03
12- 04-03
01-06-04
02-03-04
03-03-04
04-06-04
05-04-04
3.0
2.9
2.9
2.9
2.7
3.5
2.5
3.2
2.6
3.2
4.7
3.1
3.2
2.6
3.6
3.5
2.7
3.0
2.8
3.3
29.7
33.8
30.5
33 2
32.3
28.7
26.3
27.6
25.9
29.0
33.3
29.3
33.4
31.0
30.4
30.6
30.7
29.0
28.1
32.5
The lower and upper bounds '.‘• ere
reference toxicant tests.
Coefficient of variation for control reproduction.
On average, the CV for control reproduction is 7.7% in Environmental Testing Solutions, Inc. Ceriodaphnia chronic toxicity
tests.
Lower CV bound determined by USEPA (10*' percentile) = 8.9o/o.
Upper CV bound determined by USEPA (90dl percentile) = 42%
MSD = MinimumSignificantDifrerer.ee
PMSD = Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can
be declared statistically significant in a whole effluent toxicity lest On average, a significant difference occurs for
Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 10.2%
from the control.
Lower PMSD bound determined by USEPA (IO41 percentile) = 11%.
Upper PMSD bound determined by USEPA (90d' percentile) = 37%.
CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD)
August 27, 2004
RE:ETS PROJECT NUMBER: 1413
Dear Mr. Thrower:
Test Procedure
EPA-821-R-02-013TGP3B PASS
If you have any questions concerning these results, please feel free to contact me.
Additionally, please sign and submit the enclosed DWQ Aquatic Toxicity Form (AT-1)
to the following address. The original AT-1 form must be mailed by
September 30, 2004.
If this test was performed as an NPDES requirement or by Administrative Letter, please
enter a P on the Effluent Discharge Monitoring Form (MR-1) for the collection date
August 16, 2004 using the parameter code TGP3B.
Enclosed are toxicity test results for samples from the Durham County Triangle
WWTP received by Environmental Testing Solutions, Inc. August 18 through
August 21, 2004.
North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
Parameter
Code
North Carolina Ceriodaphnia Chronic Effluent
Toxicity Procedure - December 1985, Revised:
February 1998
(Ceriodaphnia Pass/Fail Toxicity Test)
Final
Result
Mi". Link Thrower
Chemical & Environmental Technology, Inc.
102-A Woodwinds Industrial Court
Cary, NC 27511
POBox 7565
Asheville, NC 28802
EPA Method
Number
North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
Sincerely,
c>/^—
Jim Sumner
Laboratory Director
Environmental Testing Solutions, Inc.________
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Date:August 27, 2004
Pipe #: 001 County Durham
Comments: .
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent
90%
Pass Fail
X
Test Start Date:08-18-04
Sample 2 08-19-04
Duration
24-h
24-h
33
Control
High Cone.
pH (S.U.)DO (mg/L)Organism Tested:Ceriodaphnia dubia Duration:7-days
DWQform AT-1 (3/87) rev. 11/95
Concentration (%)
Mortality (%)
PO Box 7565
Asheville, NC 28802
D.O. (mg/L)
Control
Treatment 2
Method of Determination
Trimmed Spearman Karber
Probit
Other:
Sample 1
Sample 2
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO3/L)
Conductivity (umhos/cm)
Total Residual Chlorine (mg/L)
Sample Temp, at Receipt (°C)
Chronic Test Results
Calculated t:
Tabular t:
% Reduction:
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
pH (S.U.)
Control
Treatment 2
1
55
■K
ce
Treatment 2
28.9
■u
%
u
'o. E
w
T3
w
■O
■E
5 oo
E
Average
Reproduction
Control
29.3
Effluent Percentage
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
Control Organisms_________
Number of Young Produced
Adult Survival: (L^ve, (D)ead
7.76
7.94
7,6
7.4
2
29
L
2
29
L
7.88
7?96
7.9
T9
3
30
L
7,72
7.88
6
31
L
6
33
7
28
L
9
34
L
10
30
L
Project:
Samples:
Morality
Control
0.0
1413
040818.03,040821.04
0.405
2.508
1.4
1
26
L
1
31
T
3
32
T
4
30
L
4
26
L
2nd Sample
7.79
7.71
5
28
L
5
25
L
7.8
7
26
8
26
L
8
30
L
9
29
L
10
27
L
11
33
L
11
27
L
12
29
L
12
30
L
1st Sample
7.75
7.70
1st Sample
8,0
8.2
1st Sample
7,9
7.9
1st Sample
7.76
7.65
Treatment 2
0.0
Control CV
436
<0.10
3.5
9.3
% control orgar/STTB
producing 3rd brood
100
40
1”. 155.
174
Sample Type/Duration
Grab Comp.
x-
X
LCjo/Acute Toxicity Test
(Mortality expressed as %, combining replicates.)
LC50 =
95% Confidence Limits
to
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
II
X 4 “
2nd Sample
8.0
8.0
Environmental Testing Solutions, Inc.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
_____
558
<0.10
1.2
Collection (Start) Date:
Sample 1 08-16-04
Facility CET NPDES #: NC- 0026501
Durham County - Triangle WWTP
Laboratory Performing Test: Environmental Testing Solutions, Inc.
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: ------x