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HomeMy WebLinkAboutNC0026051_Chronic toxicity report_200103231 March 23, 2001 Re:Chronic Toxicity Results Please find the enclosed original with a copy of the results. If you have any questions regarding this report please contact me at (919) 544-8280. CC/Mr. Chuck Hill P.E., Durham County Mr. Jeffrey DuPont, Woodard & Curran, Inc Mr. R. Scott Lewis, Woodard & Curran, Inc Environmental Sciences Branch Division of Water Quality NC DENR 1621 Mail Service Center Raleigh, NC 27699-1621 Sincerely, Victor Cozart Chief Operator/ ORC As per our sample events on February 22, and February 27, both passed the chronic toxicity testing requirements. 3 a 2001 div. of water quality CENTRAL FILES CHAIN OF CUSTODY Page of / GRAB Client: Collected By (print):p/Uy I Signature: Date/Time:Frequency:Chilled?: Parameters Clinic TRANSPORT OF SAMPLESAMPLE CUSTODY TRANSFER RECORD (Please sign) Carrier:Sample Sealed By. RECEIPT AT LABORATORY Relinquished By: Sample Disposed/Returned By: Fieservative concentrations are below DOT concentration limits. Relinquished By: Received By: G Relinquished By: Received By. Relinquished By. Received By: Relinquished By: Received By: Organization: Organization: Organization: Organization: Organization: Organization: Date: Date:, Date: Date: Time: Time: Transport Method: Received By (Signature) Collected By (print): Signature: Date/Time: Time. Time: Organization: Date/Time: Volume Preservative '\2-^.-------- CO I Time ^ty/PM loop Log #Type L # Containers I Collector 3FZ COMPOSITE Set By (print)”T" Signature: Date/Time: Tganization: rganization: /fz?' Time: Time: Time: County: Pipe#:_ Date ^^01 Sample ID e1t ^^“^^nvlronmental, Inc. Received By: Organization: L'1 Date/Time: • Unsealed By Arrival Temp.: Date: I /■J°C Date: Date: i Date: bate: Mailing Address: P.O. Box 16414, Greenville, SC 29606-7414 Shipping Address: 4 Craftsman Court, Greer, SC 29650 Phone: (864) 877-6942 Fax: (864) 877-6938 Toll free: (800) 891-2325 Pn'siYA _______________ Site Description: State:, NPDES #: ^(COO^O^I Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date 09-Mar-2001 County: DURHAMNPDES#NC0026051 Pipe# Comments MAIL ORIGINAL TO North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test CONTROL ORGANISMS 2 3 4 5 7 9 10 11 12168 21 16 24 21 24 18 23 27 27 23 25 26# Young Produced % Mortality Avg. Reprod. L L L LLLLL L L L LAdult (L)ive (D)ead Effluent %90.00%0%26.9 Treatment 2Treatment 2 Control CV 14.9%TREATMENT 2 ORGANISMS 1 2 3 5 6 7 8 9 10 11 124 27 28 30 26 29 24 242725252929 % 3rd Brood PASS FAIL# Young Produced XL92%L L L L L L L L L L LAdult (L)ive (D)ead Complete This for Either Test Test Start Date Collection (Start) Date 2-23-01 PH 2nd sample Sample 1 2-21 2001 Sample 2 2-26 20011st sample 1st sample Control 7.0 7.4 7.5 7.87.3 7.3 Sample Type (Duration) Treatment 2 8.0 7.1 7.57.1 7.2 7.0 2ndGrabDuration1st Tox Tox24hrs.Sample 1 X Sample SampleDilutionSample 2 24hrs.end end start endstartstart D.O.50.0Hardness (mg/L)1st sample 1st sample 2nd sample 6476589.7 8.2 8.0 8.1Control8.8 9.5 Spec. Cond. (pmhos) 0.09<0.0510.2 8.1Treatment 2 9.4 9.5 8.88.8 Chlorine (mg/L) 2.11.2Sample Temp, at receipt (°C) LC50/Acute Toxicity Test (Mortality expressed as %, combining replicates) Concentration start/endstart/endMortality Method of Determination ControlLC50 = Probit High Cone.95% Confidence Limits Moving Average D.O.pHOther% Organism Tested DEM Form AT-1 ETT Environmental, Inc. Facility: WOODARD & CURRAN EFFLUENT Laboratory Performing Test: X 0% Control 22.9 Control -3.45 2.508 -17% Comp 266 __________ Signature of Operator in Responsibly Charge x___________ Signature of Laboratory Supervisor Spearman Karber Ceriodaphnia dubia Environmental Sciences Branch Ri?.WrQua,i,y1621 Mail Service Center Raleigh, North Carolina 27699-1621 Chronic Test Results Calculated t= Critical Value= % Reduction= STATISTICAL ANALYSIS RESULTS WOODARD & CURRAN NC0026051NPDES# EFFLUENT Date:2-23-01ETTST16633 Certification #: NC022 11/2001Exp. Date: Survival Data 7 Day Survival Test Used:FISHERS TEST 1.000100%Test Statistic:Control P= 100%0.01EffluentCritical Value:P= PASS: The effluent does not reduce survival of the test organisms. Reproduction Data Raw Data Test for Normality Mean young/female Std. Dev.Test Used:Shapiro-Wilks Test: Control 22.9 3.42 Effluent 0.95226.9 2.11 W: Critical Value:0.884 Analysis for Differences in Reproduction Test Used:Test Used:Equal Variance t Test.F Test 2.64Calculated t=-3.45 F= 5.32Critical Value=Critical Value=2.51 The data are homogeneous in variance PASS: The effluent is not chronically toxic. QUALITY CONTROL RESULTS Reference Toxicant: NaCl Current Chronic NOEC:1.96Current Acute LC50: Mean Chronic NOEC::2.01Mean Acute LC50: 1.50.41.75 2.22 Lower & Upper Limits (g/L)Lower & Upper Limits (g/L) ETT Environmental, Inc? 0.8 gA 0.8 g/Lg^ Facility: Sample ID: Laboratory: The data are normally distributed. Test for Homogeneity of Variance CHAIN OF CUSTODY /Page GRAB Client: Collected By (print): Signature: Date/Time:Frequency:Chilled?: Parameters I SAMPLE CUSTODY TRANSFER RECORD (Please sign)TRANSPORT OF SAMPLE Sample Sealed By.Carrier: RECEIPT AT LABORATORY Relinquished By: Sample Disposed/Returned By: Fieservative concentrations are below DOT concentration limits. Relinquished By: Received By: Relinquished By: Received By: (2 Relinquished By: Received By: Relinquished By Received By: Organization: Organization: Organization: Organization: Organization: Organization: Date: Date: Date: Date: Time: Time: Time: Time: Time: Time: Transport Method: Received By (Signature)/ Collected By (print): Signature: Date/Time: Type Volume Preservative ------ Log # (90/ Time ^/PMCollector # Containers I Organization: Date/Time: irganization: iganization: i: Time: / ^^/Time:/^^ < nos °c COMPOSITE Set By (printjT Signature: Date/Time: County: Pipe#: _ DateSample ID gm Received By: Organization: 1 Date/Time: Unsealed By: fid-''!?-' Arrival Temp.: Date: E1T ^^""nvlronmental, Inc. P/Uy I Date: Date: > Date: Date: Mailing Address: P.O. Box 16414, Greenville, SC 29606-7414 Shipping Address: 4 Craftsman Court, Greer, SC 29650 Phone:(864) 877-6942 Fax:(864) 877-6938 Toll free: (800) 891-2325 Pri'snr _______________ Site Description: State: P NPDES #: I of Lnotzrc JtL I r n a Ir test fax He. 4995 P. 1 Pace 1 of 1 Laboratory Report Work Order#;0105-00135 QuekfterMethodResultsTest Performed 5/16/01 15:44NG Pft»s/Fail PASSBiaaseay, Chronic Paat/Fell Reviewed by: for Tritest, Inc, 6701 Conference Drive Raleigh, NC 27607 Time Sampled 11;5& Report Dete: Date Raceived: Matrix ww NCWW Cert, ft: 067 NC/DW Cert, f: 37731 CH3O0O 190 Sample Type Composite 5/31/2001 6/2/2001 Analyzed Date Time Condition See COC Telephone: (919) 834-4M4 Fax: (819)834-6497 Sample ID EFFLUENT /PIPE 001 No 001 Prefect No.: 01 Project ID: TRIANGLE WWTP! BIOASSAY Date Sampled 5/2/2001 CU9t. Coda: Cust. P.O.#: 0 WoTEST UCC £1 CUUO lUZO'+NI'l ■ ■ OcL 2 1. 2005 10:W - Prepared for — Mr. Link Thrower C SET 102-A Woodwinds Industrial Ct. Cary, NC 27511 Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 08/25/01 Facility: TRIANGLE WWTP NPDEStr : NC0026051 Pipe# :001 County: DURHAM INC. MAIL ORIGINAL TO: CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Mortality Avg.Reprod. # Young Produced 30 24 25 32 31 27 30 29 29 29 31 27 Adult (L)ive (D)ead L L L L L L L L L L L L 1 2 3 6 8 9 10 11 12457 PASS FAIL # Young Produced 26 30 26 28 31 24 27 26 30 26 X2527 Check One Adult (L)ive (D)ead L L L L L L L L L LLL 1st sample 1st s amp1e 2nd sample PH Control 7.87.9 8.0 7.9 8.1 7.8 08/09/01 7.6Treatment 2 7.6 7.6 7.6 7.7 7.7 1st DurationGrabComp. 24 hrsSample 1 X 24 hrsSample 2 X Hardness(mg/1)42 8.08.0 7.8 7.98.1 7.9 826Spec. Cond. (/zmhos)984170 8.0 8.2 8.0Treatment 2 8.1 8.0 8.1 <0.1Chlorine(mg/1)<0.1 2.03.5Sample temp, at receipt(°C)LC50/Acute Toxicity Test (Mortality expressed as %, combining replicates) Concentration%%%%%%%%%% %%%%%%%%%%start/endtart/end Control Other D.O.PH Duration(hrs): Ceriodaphnia dubiaOrganism Tested: Copied from DEM form AT-1 (3/87) High Cone . S A M P S A M P 0.00 Treatment 2 Control CV 8.588% 0.00 Control D I L U T 27.17 Treatment 2 28.67 Control 2nd P/F Effluent %: 90? TREATMENT 2 ORGANISMS LC5 0 = % 95% Confidence Limits % -- % e n d e n d e n d Mortality s D.O. Control s t a r t 1st sample s t a r t 1st sample s t a r t 2nd sample % control orgs producing 3rd brood 100% I Note: Please Complete This Section Also North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Environmental Sciences Branch Div. of Environmental Management N.C. Dept, of EHNR 4401 Reedy Creek Road Raleigh, North Carolina 27607-6445 Chronic Test Results Calculated t = 1.584 Tabular t = 2.508 % Reduction = 5.23 Complete This For Either Test Test Start Date: 08/08/01 Collection (Start) Date Sample 1: 08/06/01 Sample 2: Sample Type/Duration Comments: CE & T Laboratory Performing Test: TRITEST, ______________ Signature ofADrerator/ in Responsible Charge x ASig'nartfuye rof'"Trhboratory Supervisor Work Order: 0108-00306 Method of Determination Moving Average Probit Spearman Karber * PASSED: 5.23% Reduction rev. 11/95 (DUBIA ver. 4.32) Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 12/11/01 Facility: TRIANGLE WWTP NPDES#: NC0026051 Pipe#: 001 County: DURHAM Laboratory Performing Test: TRITEST,INC. Comments: a MAIL ORIGINAL TO: CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Mortality Avg.Reprod. # Young Produced 29 29 30 24 26 27 31 33 28 27 28 27 Adult (L)ive (D)ead L L L L L L L L L L L L 1 2 3 4 5 6 7 8 9 10 11 12 PASS FAIL # Young Produced 26 27 28 32 29 25 25 30 29 28 32 31 X Adult (L)ive (D)ead L L L L L LL L L L L L 1st sample 1st sample 2nd sample PH Control 8.1 8.2 7.9 8.2 8.0 7.9 Sample 2: 11/29/01 Treatment 2 7.8 7.8 7.7 7.8 7.5 7.7 1st Grab DurationComp. Sample 1 24 hrsX Sample 2 24 hrsX Hardness(mg/1)50 8.3 8 . 0 8.2 8.0 8.2 7.9 Spec. Cond . (/imhos)740698211 Treatment 2 8.2 7.9 8.1 8.0 8.3 7.9 Chlorine(mg/1)<0.1<0.1 LC50/Acute Toxicity Test Sample temp, at receipt(°C)3.42.1 %% %%%%%Concentration%%% %%%%%%%%%% start/end Control D.O .P-H Organism Tested:Ceriodaphnia dubia Duration(hrs) : Copied from DEM form AT-1 (3/87)rev. Method of Determination Probit Other Moving Average Spearman Karber Complete This For Either Test Test Start Date: 11/28/01 Collection (Start) Date Sample 1: 11/26/01 . Sample Type/Duration High Cone . I Note: Please Complete This Section Also D I L U T S A M P S A M P 0.00 Treatment 2 Control CV 8.421% 0 . 00 Control 28.25 Control LC50.= % 95% Confidence Limits % -- % e n d 28.50 Treatment 2 2nd P/F e n d e n d Environmental Sciences Branch Div. of Environmental Management N.C. Dept, of EHNR 4401 Reedy Creek Road Raleigh, North Carolina 27607-6445 D.O. Control Effluent %: 90% TREATMENT 2 ORGANISMS s t a r t 1st sample s t a r t 1st sample s t a r t 2nd sample Check One North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test ZU- _________ tator m Responsible Cnarge Drat5<5ry /Supervisor Mortality start/end % control orgs producing 3rd brood 100% X__ ( Sig. X__ Sig: Chronic Test Results Calculated t = -0.253 Tabular t = 2.508 % Reduction = -0.88 (Mortality expressed as %, combining replicates) Work Order: 0111-01441 11/95 (DUBIA ver. 4.32) * PASSED: -0.88% Reduction TRITEST, INC. Bioassay Chain of Custody Form Bioassays-are to be sampled on P.O. #: Outfall/Pipe# _• Signature > Date Started : Time : or PM Date Ended : 10^5Time:'or PM Samples per Hour: '■ # Hours : Grab : Date : Time : Sample Volume: Received By For Laboratory Use Only £■/Receiving Temperature: By. Residual Chlorine: Number of Bottles: Address: 3909 Beryl Rd.- Raleigh, NC 27607 Phone(919) 834 -4984 Fax (919) 834-6497 / / Date______ // Z 7 o / DateRelinquished By /* —> lime Method of T ransportation to Lab : C^'T" C/)()rri(,r D/cJ^ ■ UD ....../ 7 Chain of Custody Release after 8:30a.m. Tuesday & Friday. -CO I.------- NPDES Permit # : Test Method : Pas^/F(j/I ___ Facility Name: "PpiOn^e. \A\/TP Address:, //< ------Oufiarri, NC. £-77/3 Phone#( 97/ ■ ) JNN-NM Contact '■ Csirolun Mintnri 1 ime Received By ,/zZ^____ AM or PM / ■ Chilled during Collection? f — County %Effluent Dilution : Sample Site Description: 0///37/)£ - (T>. Sample Collector: Print > Nd! A. (MNwNI Sample rype: Comoosite : TRITEST, INC. Bioassay Chain of Custody Form Phone 3 ( W/ ) Contact: ScM County: Outfall/ Pipe #00/ NPDES Permit U: rfg Time : Date Ended :Time : # Hours : Time : Sample Volume: For Laboratory Use Only 0 Receiving Temperature: By: Residual Chlorine: Number of Bottles : Signature > Sample Type: Composite ; Date Started : Dur^oro $ v'l Relinquished By Time Received ByDate //ho/o/ Chilled during Collection? y^5 t************* ♦ ♦ ♦ * **A** Method of Transportation to Lab : Q£T Courrtor pick OD ...........................Chain of Custody Release ................................ Addres: 3909 Beryl Rd.- Raleigh, NC 27607 Phone(919) 834 -4984 Fax (919) 834-6497 nllOr^l^l Test Method: Pqc^ q^c/ ' / —---------- Sample Site Description: -QciCjn^ P Etf/nsr/. Sample Collector: Print> Nfi! P- O’Driw/J OU CP ll/.vlnl______'■ Samples per Hour : Grab ; Date :A/A Facility Name: tyirlwO Gx - 'Trir^P W7p Address: p-yp/-, pJC Pur Pm; /,/Q z 77/ 3 I Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 03/14/02 NPDES#: NC0026051 Pipe#: Labora INC . Comments: Resubmited copy X Responsible Charge X Supervisor * PASSED: Work 0202-00791 MAIL ORIGINAL TO: CONTROL ORGANISMS 1 2 3 645 7 8 9 10 11 12 % Mortality Avg.Reprod. # Young Produced 27 33 27 21 30 25 28 29 22 33 26 30 Adult (Dive (D)ead L L L L L L L L L L L L 1 2 3 4 5 6 7 8 9 10 11 12 PASS FAIL # Young Produced 32 31 34 35 33 36 3.3 33 3338 34 32 X Check One I Adult (L)ive (D)ead L L L L L L L L L L L L 1st sample 1st sample 2nd sample pH Control 7.9 7.9 7.8 7.9 7.8 7.9 Treatment 2 7.4 7.6 7.2 8.0 7.8 7.8 1st Grab DurationComp . Sample 1 24 hrsX Sample 2 24 hrsX Hardness(mg/1)50 8 . 0 8 . 0 8.2 8.0 8.3 7.9 753Spec. Cond.(umhos)711218 8.3 8.0 8.3 7.9 8.2 8 . 2Treatment 2 Chlorine(mg/1)<0.1<0.1 LC50/Acute Toxicity Test 1.7Sample temp, at receipt(°C)0.9 (Mortality expressed as %, combining replicates) Concentration%%% %%%%%%% %%%%%%%%%% start/end Control PH D.O. Organism Tested:Ceriodaphnia dubia Duration(hrs): 11/95 (DUBIA ver.4.32)Copied from DEM form AT-1 (3/87)rev. North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Method of Determination Moving Average Spearman Karber Probit Other High Cone . D I L U T S A M P S A M P S t a 0.00 Treatment 2 Control CV 13.693% 33.67 Treatment 2 27.58 Control 2nd P/F LC5 0 = % 95% Confidence Limits % -- % e n d e n d 0.00 Control e n d Mortality start/end % control orgs producing 3rd brood 100% ^ider:Environmental Sciences Branch Div. of Environmental Management N.C. Dept, of EHNR 4401 Reedy Creek Road Raleigh, North Carolina 27607-6445 D.O. Control )era.tor ica n»'J (oor^tory . I Note: Please Complete This Section Also s t a r t 1st sample s t a r t 1st sample t 2nd sample Effluent %: 90% TREATMENT 2 ORGANISMS Chronic Test Results Calculated t = -4.972 Tabular t = 2.508 % Reduction = -22.05 -22.05% Reduction * Complete This For Either Test Test Start Date: 02/20/02 Collection (Start) Date Sample 1: 02/18/02 Sample 2: 02/21/02 Sample Type/Duration 001 County: DURHAM yQPerforming Test: TRITEST, rfe' o facility: TRIANGLE WWTP June 15,2001 Chronic Toxicity ResultsRe: Please find the enclosed original with a copy of the results. If you have any questions regarding this report please contact me at (919) 544-8280. Triangle Wastewater Treatment Facility i 5926 Highway 55 East i Durham, North Carolina 27713 919-544-8280 i 919-544-8590 (Fax) i www.woodardcurran.com CORPORATE OFFICES: Maine, Massachusetts, New Hampshire, Connecticut, Illinois, Florida Operational offices throughout the U.S. Environmental Sciences Branch Division of Water Quality NC DENR 1621 Mail Service Center Raleigh, NC 27699-1621 WOODARD &CURRAN Engineering • Science < Operations Sincerely, Victor Cozart Chief Operator/ORC As per our sample events on May 20, and May 23, both passed the chronic toxicity testing requirements. CC/ Mr, Chuck Hill P.E., Durham County ^•Nfr. Jerry Rimmer, Woodard & Curran, Inc Mr. R. Scott Lewis, Woodard & Curran, Inc Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 06/10/02 Facility: TRIANGLE WWTP NPDES#: NC0026051 Pipe#:001 County: DURHAM erforming Test: TRITEST,INC . Comments: -0.88% Reduction * 0205-00905 MAIL ORIGINAL TO: CONTROL ORGANISMS 1 2 3 7456 8 9 10 11 12 % Mortality Avg.Reprod. # Young Produced 32 24 24 28 29 29 30 27 32 29 27 30 Adult (L)ive (D)ead L L L L L L L L L L L L 21 3 4 5 6 7 8 10 11 129 PASS FAIL # Young Produced 31 33 30 33 30 25 33 26 21 28 25 29 X Check. One Adult (L)ive (D)ead L L L L L L L L L L L L 1st sample 1st sample 2nd sample pH Control 8.1 8.3 8 .2 8.2 8.1 8.0 Sample 2: 05/23/02 Treatment 2 7.8 7.7 7.2 7.8 7.7 7.8 1st Grab DurationComp . Sample 1 2 4 hrsX Sample 2 24 hrsX Hardness(mg/1)48 8.5 8.5 8.5 7.9 8.4 7.9 893Spec. Cond. (/zmhos)493178 Treatment 2 8.2 8.5 8.58.4 7.8 8.0 Chlorine(mg/1)<0.1<0.1 1.7LC50/Acute Toxicity Test Sample temp, at receipt(°C)3.1 (Mortality expressed as %, combining replicates) Concentration% %%%%%%%%% %% %%%%%%%% start/end Control D.O.PH Ceriodaphnia dubia Duration(hrs): High Cone . Method of Determination Moving Average Spearman Karber Probit Other S A M P S A M P Control CV 9.184% 0.00 Control D I L U T 28.67 Treatment 2 28.42 Control 2nd P/F LC5 0 = _ ______% 95% Confidence Limits % -- % s t a e n d 0.00 Treatment 2 e n d e n d Mortality start/end % control orgs producing 3rd brood 92% Environmental Sciences Branch Div. of Water Quality N.C. DENR 1621 Mail Service Center Raleigh, North Carolina 27699-1621 cq _______ ___ ator in Responsible Charge ofvLabdratory Supervisor D.O. Control t 1st sample s t a r t 1st sample s t a r t 2nd sample | Note: Please Complete This Section Also Effluent %: 90% TREATMENT 2 ORGANISMS Laboratory Pe Siqhatuth c North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Complete This For Either Test Test Start Date: 05/22/02 Collection (Start) Date Sample 1: 05/20/02 : Sample Type/Duration I Organism Tested: Copied from DWQ form AT-1 (3/87) Work Order: Chronic Test Results Calculated t = -0.188 Tabular t = 2.508 % Reduction = -0.88 X__/ ' “W * PASSED: rev. 11/95 (DUBIA ver. 4.41)