HomeMy WebLinkAboutNC0026051_Chronic toxicity report_200103231
March 23, 2001
Re:Chronic Toxicity Results
Please find the enclosed original with a copy of the results.
If you have any questions regarding this report please contact me at (919) 544-8280.
CC/Mr. Chuck Hill P.E., Durham County
Mr. Jeffrey DuPont, Woodard & Curran, Inc
Mr. R. Scott Lewis, Woodard & Curran, Inc
Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Sincerely,
Victor Cozart
Chief Operator/ ORC
As per our sample events on February 22, and February 27, both passed the chronic
toxicity testing requirements.
3 a 2001
div. of water quality
CENTRAL FILES
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rganization: /fz?'
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^^01
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e1t
^^“^^nvlronmental, Inc.
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Date:
I
/■J°C
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i
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bate:
Mailing Address: P.O. Box 16414, Greenville, SC 29606-7414
Shipping Address: 4 Craftsman Court, Greer, SC 29650
Phone: (864) 877-6942 Fax: (864) 877-6938 Toll free: (800) 891-2325
Pn'siYA _______________
Site Description:
State:,
NPDES #: ^(COO^O^I
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date 09-Mar-2001
County: DURHAMNPDES#NC0026051 Pipe#
Comments
MAIL ORIGINAL TO
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
2 3 4 5 7 9 10 11 12168
21 16 24 21 24 18 23 27 27 23 25 26# Young Produced % Mortality Avg. Reprod.
L L L LLLLL L L L LAdult (L)ive (D)ead
Effluent %90.00%0%26.9
Treatment 2Treatment 2
Control CV
14.9%TREATMENT 2 ORGANISMS 1 2 3 5 6 7 8 9 10 11 124
27 28 30 26 29 24 242725252929 % 3rd Brood PASS FAIL# Young Produced
XL92%L L L L L L L L L L LAdult (L)ive (D)ead
Complete This for Either Test Test Start Date
Collection (Start) Date 2-23-01
PH 2nd sample Sample 1 2-21 2001 Sample 2 2-26 20011st sample 1st sample
Control 7.0 7.4 7.5 7.87.3 7.3 Sample Type (Duration)
Treatment 2 8.0 7.1 7.57.1 7.2 7.0 2ndGrabDuration1st
Tox Tox24hrs.Sample 1
X Sample SampleDilutionSample 2 24hrs.end end start endstartstart
D.O.50.0Hardness (mg/L)1st sample 1st sample 2nd sample
6476589.7 8.2 8.0 8.1Control8.8 9.5 Spec. Cond. (pmhos)
0.09<0.0510.2 8.1Treatment 2 9.4 9.5 8.88.8 Chlorine (mg/L)
2.11.2Sample Temp, at receipt (°C)
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
Concentration
start/endstart/endMortality
Method of Determination ControlLC50 =
Probit High Cone.95% Confidence Limits Moving Average
D.O.pHOther%
Organism Tested
DEM Form AT-1
ETT Environmental, Inc.
Facility: WOODARD & CURRAN EFFLUENT
Laboratory Performing Test:
X
0%
Control
22.9
Control
-3.45
2.508
-17%
Comp
266
__________
Signature of Operator in Responsibly Charge
x___________
Signature of Laboratory Supervisor
Spearman Karber
Ceriodaphnia dubia
Environmental Sciences Branch
Ri?.WrQua,i,y1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Chronic Test Results
Calculated t=
Critical Value=
% Reduction=
STATISTICAL ANALYSIS RESULTS
WOODARD & CURRAN NC0026051NPDES#
EFFLUENT Date:2-23-01ETTST16633
Certification #: NC022 11/2001Exp. Date:
Survival Data
7 Day Survival Test Used:FISHERS TEST
1.000100%Test Statistic:Control P=
100%0.01EffluentCritical Value:P=
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data Test for Normality
Mean young/female Std. Dev.Test Used:Shapiro-Wilks Test:
Control 22.9 3.42
Effluent 0.95226.9 2.11 W:
Critical Value:0.884
Analysis for Differences in Reproduction
Test Used:Test Used:Equal Variance t Test.F Test
2.64Calculated t=-3.45 F=
5.32Critical Value=Critical Value=2.51
The data are homogeneous in variance
PASS: The effluent is not chronically toxic.
QUALITY CONTROL RESULTS
Reference Toxicant: NaCl
Current Chronic NOEC:1.96Current Acute LC50:
Mean Chronic NOEC::2.01Mean Acute LC50:
1.50.41.75 2.22 Lower & Upper Limits (g/L)Lower & Upper Limits (g/L)
ETT Environmental, Inc?
0.8 gA
0.8 g/Lg^
Facility:
Sample ID:
Laboratory:
The data are normally distributed.
Test for Homogeneity of Variance
CHAIN OF CUSTODY
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Client:
Collected By (print):
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Parameters
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SAMPLE CUSTODY TRANSFER RECORD (Please sign)TRANSPORT OF SAMPLE
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RECEIPT AT LABORATORY
Relinquished By:
Sample Disposed/Returned By:
Fieservative concentrations are below DOT concentration limits.
Relinquished By:
Received By:
Relinquished By:
Received By: (2
Relinquished By:
Received By:
Relinquished By
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Organization:
Organization:
Organization:
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Date:
Date:
Date:
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Time:
Time:
Time:
Transport Method:
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Collected By (print):
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------
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(90/
Time ^/PMCollector # Containers
I
Organization:
Date/Time:
irganization:
iganization:
i: Time: /
^^/Time:/^^
< nos
°c
COMPOSITE
Set By (printjT
Signature:
Date/Time:
County:
Pipe#: _
DateSample ID
gm
Received By:
Organization: 1
Date/Time:
Unsealed By: fid-''!?-'
Arrival Temp.:
Date:
E1T
^^""nvlronmental, Inc.
P/Uy I
Date:
Date:
>
Date:
Date:
Mailing Address: P.O. Box 16414, Greenville, SC 29606-7414
Shipping Address: 4 Craftsman Court, Greer, SC 29650
Phone:(864) 877-6942 Fax:(864) 877-6938 Toll free: (800) 891-2325
Pri'snr _______________
Site Description:
State: P
NPDES #:
I of
Lnotzrc JtL I r n a
Ir test fax He. 4995 P. 1
Pace 1 of 1
Laboratory Report
Work Order#;0105-00135
QuekfterMethodResultsTest Performed
5/16/01 15:44NG Pft»s/Fail PASSBiaaseay, Chronic Paat/Fell
Reviewed by:
for Tritest, Inc,
6701 Conference Drive
Raleigh, NC 27607
Time Sampled
11;5&
Report Dete:
Date Raceived:
Matrix
ww
NCWW Cert, ft: 067
NC/DW Cert, f: 37731
CH3O0O
190
Sample Type
Composite
5/31/2001
6/2/2001
Analyzed
Date Time
Condition
See COC
Telephone: (919) 834-4M4
Fax: (819)834-6497
Sample ID
EFFLUENT /PIPE 001
No
001
Prefect No.: 01
Project ID: TRIANGLE WWTP! BIOASSAY
Date Sampled
5/2/2001
CU9t. Coda:
Cust. P.O.#:
0
WoTEST
UCC £1 CUUO lUZO'+NI'l
■ ■ OcL 2 1. 2005 10:W
- Prepared for —
Mr. Link Thrower
C SET
102-A Woodwinds Industrial Ct.
Cary, NC 27511
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 08/25/01
Facility: TRIANGLE WWTP NPDEStr : NC0026051 Pipe# :001 County: DURHAM
INC.
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
% Mortality Avg.Reprod.
# Young Produced 30 24 25 32 31 27 30 29 29 29 31 27
Adult (L)ive (D)ead L L L L L L L L L L L L
1 2 3 6 8 9 10 11 12457
PASS FAIL
# Young Produced 26 30 26 28 31 24 27 26 30 26 X2527
Check One
Adult (L)ive (D)ead L L L L L L L L L LLL
1st sample 1st s amp1e 2nd sample
PH
Control 7.87.9 8.0 7.9 8.1 7.8
08/09/01
7.6Treatment 2 7.6 7.6 7.6 7.7 7.7
1st
DurationGrabComp.
24 hrsSample 1 X
24 hrsSample 2 X
Hardness(mg/1)42
8.08.0 7.8 7.98.1 7.9
826Spec. Cond. (/zmhos)984170
8.0 8.2 8.0Treatment 2 8.1 8.0 8.1 <0.1Chlorine(mg/1)<0.1
2.03.5Sample temp, at receipt(°C)LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
Concentration%%%%%%%%%%
%%%%%%%%%%start/endtart/end
Control
Other
D.O.PH
Duration(hrs): Ceriodaphnia dubiaOrganism Tested:
Copied from DEM form AT-1 (3/87)
High
Cone .
S
A
M
P
S
A
M
P
0.00
Treatment 2
Control CV
8.588%
0.00
Control
D
I
L
U
T
27.17
Treatment 2
28.67
Control
2nd
P/F
Effluent %: 90?
TREATMENT 2 ORGANISMS
LC5 0 = %
95% Confidence Limits
% -- %
e
n
d
e
n
d
e
n
d
Mortality
s
D.O.
Control
s
t
a
r
t
1st sample
s
t
a
r
t
1st sample
s
t
a
r
t
2nd sample
% control orgs
producing 3rd
brood
100%
I
Note: Please
Complete This
Section Also
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
Environmental Sciences Branch
Div. of Environmental Management
N.C. Dept, of EHNR
4401 Reedy Creek Road
Raleigh, North Carolina 27607-6445
Chronic Test Results
Calculated t = 1.584
Tabular t = 2.508
% Reduction = 5.23
Complete This For Either Test
Test Start Date: 08/08/01
Collection (Start) Date
Sample 1: 08/06/01 Sample 2:
Sample Type/Duration
Comments: CE & T
Laboratory Performing Test: TRITEST,
______________
Signature ofADrerator/ in Responsible Charge
x ASig'nartfuye rof'"Trhboratory Supervisor
Work Order: 0108-00306
Method of Determination
Moving Average Probit
Spearman Karber
* PASSED: 5.23% Reduction
rev. 11/95 (DUBIA ver. 4.32)
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 12/11/01
Facility: TRIANGLE WWTP NPDES#: NC0026051 Pipe#: 001 County: DURHAM
Laboratory Performing Test: TRITEST,INC.
Comments: a
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
% Mortality Avg.Reprod.
# Young Produced 29 29 30 24 26 27 31 33 28 27 28 27
Adult (L)ive (D)ead L L L L L L L L L L L L
1 2 3 4 5 6 7 8 9 10 11 12
PASS FAIL
# Young Produced 26 27 28 32 29 25 25 30 29 28 32 31 X
Adult (L)ive (D)ead L L L L L LL L L L L L
1st sample 1st sample 2nd sample
PH
Control 8.1 8.2 7.9 8.2 8.0 7.9
Sample 2: 11/29/01
Treatment 2 7.8 7.8 7.7 7.8 7.5 7.7
1st
Grab DurationComp.
Sample 1 24 hrsX
Sample 2 24 hrsX
Hardness(mg/1)50
8.3 8 . 0 8.2 8.0 8.2 7.9
Spec. Cond . (/imhos)740698211
Treatment 2 8.2 7.9 8.1 8.0 8.3 7.9
Chlorine(mg/1)<0.1<0.1
LC50/Acute Toxicity Test Sample temp, at receipt(°C)3.42.1
%% %%%%%Concentration%%%
%%%%%%%%%%
start/end
Control
D.O .P-H
Organism Tested:Ceriodaphnia dubia Duration(hrs) :
Copied from DEM form AT-1 (3/87)rev.
Method of Determination
Probit
Other
Moving Average
Spearman Karber
Complete This For Either Test
Test Start Date: 11/28/01
Collection (Start) Date
Sample 1: 11/26/01 .
Sample Type/Duration
High
Cone .
I
Note: Please
Complete This
Section Also
D
I
L
U
T
S
A
M
P
S
A
M
P
0.00
Treatment 2
Control CV
8.421%
0 . 00
Control
28.25
Control
LC50.= %
95% Confidence Limits
% -- %
e
n
d
28.50
Treatment 2
2nd
P/F
e
n
d
e
n
d
Environmental Sciences Branch
Div. of Environmental Management
N.C. Dept, of EHNR
4401 Reedy Creek Road
Raleigh, North Carolina 27607-6445
D.O.
Control
Effluent %: 90%
TREATMENT 2 ORGANISMS
s
t
a
r
t
1st sample
s
t
a
r
t
1st sample
s
t
a
r
t
2nd sample
Check One
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
ZU- _________
tator m Responsible Cnarge
Drat5<5ry /Supervisor
Mortality
start/end
% control orgs
producing 3rd
brood
100%
X__ (
Sig.
X__
Sig:
Chronic Test Results
Calculated t = -0.253
Tabular t = 2.508
% Reduction = -0.88
(Mortality expressed as %, combining replicates)
Work Order: 0111-01441
11/95 (DUBIA ver. 4.32)
* PASSED: -0.88% Reduction
TRITEST, INC.
Bioassay Chain of Custody Form
Bioassays-are to be sampled on
P.O. #:
Outfall/Pipe# _•
Signature >
Date Started : Time : or PM
Date Ended : 10^5Time:'or PM
Samples per Hour: '■ # Hours :
Grab :
Date : Time :
Sample Volume:
Received By
For Laboratory Use Only
£■/Receiving Temperature: By.
Residual Chlorine: Number of Bottles:
Address: 3909 Beryl Rd.- Raleigh, NC 27607 Phone(919) 834 -4984 Fax (919) 834-6497
/ /
Date______
// Z 7 o /
DateRelinquished By
/* —>
lime
Method of T ransportation to Lab : C^'T" C/)()rri(,r D/cJ^ ■ UD
....../ 7
Chain of Custody Release
after 8:30a.m.
Tuesday & Friday.
-CO I.------- NPDES Permit # :
Test Method : Pas^/F(j/I
___
Facility Name: "PpiOn^e. \A\/TP
Address:, //<
------Oufiarri, NC. £-77/3 Phone#( 97/ ■ ) JNN-NM
Contact '■ Csirolun Mintnri
1 ime Received By
,/zZ^____ AM or PM
/ ■
Chilled during Collection?
f —
County
%Effluent Dilution :
Sample Site Description: 0///37/)£ - (T>.
Sample Collector: Print > Nd! A. (MNwNI
Sample rype: Comoosite :
TRITEST, INC.
Bioassay Chain of Custody Form
Phone 3 ( W/ )
Contact: ScM
County:
Outfall/ Pipe #00/
NPDES Permit U: rfg
Time :
Date Ended :Time :
# Hours :
Time :
Sample Volume:
For Laboratory Use Only
0
Receiving Temperature:
By:
Residual Chlorine: Number of Bottles :
Signature >
Sample Type: Composite ;
Date Started :
Dur^oro
$
v'l
Relinquished By Time Received ByDate
//ho/o/
Chilled during Collection? y^5
t************* ♦ ♦ ♦ * **A**
Method of Transportation to Lab : Q£T Courrtor pick OD
...........................Chain of Custody Release ................................
Addres: 3909 Beryl Rd.- Raleigh, NC 27607 Phone(919) 834 -4984 Fax (919) 834-6497
nllOr^l^l
Test Method: Pqc^ q^c/ ' / —----------
Sample Site Description: -QciCjn^ P Etf/nsr/.
Sample Collector: Print> Nfi! P- O’Driw/J
OU CP
ll/.vlnl______'■
Samples per Hour :
Grab ;
Date :A/A
Facility Name: tyirlwO Gx - 'Trir^P W7p
Address: p-yp/-, pJC
Pur Pm; /,/Q z 77/ 3
I Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 03/14/02
NPDES#: NC0026051 Pipe#:
Labora INC .
Comments: Resubmited copy
X
Responsible Charge
X
Supervisor * PASSED:
Work 0202-00791
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 645 7 8 9 10 11 12
% Mortality Avg.Reprod.
# Young Produced 27 33 27 21 30 25 28 29 22 33 26 30
Adult (Dive (D)ead L L L L L L L L L L L L
1 2 3 4 5 6 7 8 9 10 11 12
PASS FAIL
# Young Produced 32 31 34 35 33 36 3.3 33 3338 34 32 X
Check One
I Adult (L)ive (D)ead L L L L L L L L L L L L
1st sample 1st sample 2nd sample
pH
Control 7.9 7.9 7.8 7.9 7.8 7.9
Treatment 2 7.4 7.6 7.2 8.0 7.8 7.8
1st
Grab DurationComp .
Sample 1 24 hrsX
Sample 2 24 hrsX
Hardness(mg/1)50
8 . 0 8 . 0 8.2 8.0 8.3 7.9
753Spec. Cond.(umhos)711218
8.3 8.0 8.3 7.9 8.2 8 . 2Treatment 2
Chlorine(mg/1)<0.1<0.1
LC50/Acute Toxicity Test 1.7Sample temp, at receipt(°C)0.9
(Mortality expressed as %, combining replicates)
Concentration%%% %%%%%%%
%%%%%%%%%%
start/end
Control
PH D.O.
Organism Tested:Ceriodaphnia dubia Duration(hrs):
11/95 (DUBIA ver.4.32)Copied from DEM form AT-1 (3/87)rev.
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
Method of Determination
Moving Average
Spearman Karber
Probit
Other High
Cone .
D
I
L
U
T
S
A
M
P
S
A
M
P
S
t
a
0.00
Treatment 2
Control CV
13.693%
33.67
Treatment 2
27.58
Control
2nd
P/F
LC5 0 = %
95% Confidence Limits
% -- %
e
n
d
e
n
d
0.00
Control
e
n
d
Mortality
start/end
% control orgs
producing 3rd
brood
100%
^ider:Environmental Sciences Branch
Div. of Environmental Management
N.C. Dept, of EHNR
4401 Reedy Creek Road
Raleigh, North Carolina 27607-6445
D.O.
Control
)era.tor ica n»'J
(oor^tory .
I
Note: Please
Complete This
Section Also
s
t
a
r
t
1st sample
s
t
a
r
t
1st sample
t
2nd sample
Effluent %: 90%
TREATMENT 2 ORGANISMS
Chronic Test Results
Calculated t = -4.972
Tabular t = 2.508
% Reduction = -22.05
-22.05% Reduction *
Complete This For Either Test
Test Start Date: 02/20/02
Collection (Start) Date
Sample 1: 02/18/02 Sample 2: 02/21/02
Sample Type/Duration
001 County: DURHAM
yQPerforming Test: TRITEST,
rfe' o
facility: TRIANGLE WWTP
June 15,2001
Chronic Toxicity ResultsRe:
Please find the enclosed original with a copy of the results.
If you have any questions regarding this report please contact me at (919) 544-8280.
Triangle Wastewater Treatment Facility i 5926 Highway 55 East i Durham, North Carolina 27713
919-544-8280 i 919-544-8590 (Fax) i www.woodardcurran.com
CORPORATE OFFICES: Maine, Massachusetts,
New Hampshire, Connecticut, Illinois, Florida
Operational offices throughout the U.S.
Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
WOODARD &CURRAN
Engineering • Science < Operations
Sincerely,
Victor Cozart
Chief Operator/ORC
As per our sample events on May 20, and May 23, both passed the chronic toxicity
testing requirements.
CC/ Mr, Chuck Hill P.E., Durham County
^•Nfr. Jerry Rimmer, Woodard & Curran, Inc
Mr. R. Scott Lewis, Woodard & Curran, Inc
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 06/10/02
Facility: TRIANGLE WWTP NPDES#: NC0026051 Pipe#:001 County: DURHAM
erforming Test: TRITEST,INC .
Comments:
-0.88% Reduction *
0205-00905
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 7456 8 9 10 11 12
% Mortality Avg.Reprod.
# Young Produced 32 24 24 28 29 29 30 27 32 29 27 30
Adult (L)ive (D)ead L L L L L L L L L L L L
21 3 4 5 6 7 8 10 11 129
PASS FAIL
# Young Produced 31 33 30 33 30 25 33 26 21 28 25 29 X
Check. One
Adult (L)ive (D)ead L L L L L L L L L L L L
1st sample 1st sample 2nd sample
pH
Control 8.1 8.3 8 .2 8.2 8.1 8.0
Sample 2: 05/23/02
Treatment 2 7.8 7.7 7.2 7.8 7.7 7.8
1st
Grab DurationComp .
Sample 1 2 4 hrsX
Sample 2 24 hrsX
Hardness(mg/1)48
8.5 8.5 8.5 7.9 8.4 7.9
893Spec. Cond. (/zmhos)493178
Treatment 2 8.2 8.5 8.58.4 7.8 8.0
Chlorine(mg/1)<0.1<0.1
1.7LC50/Acute Toxicity Test Sample temp, at receipt(°C)3.1
(Mortality expressed as %, combining replicates)
Concentration% %%%%%%%%%
%% %%%%%%%%
start/end
Control
D.O.PH
Ceriodaphnia dubia Duration(hrs):
High
Cone .
Method of Determination
Moving Average
Spearman Karber
Probit
Other
S
A
M
P
S
A
M
P
Control CV
9.184%
0.00
Control
D
I
L
U
T
28.67
Treatment 2
28.42
Control
2nd
P/F
LC5 0 = _ ______%
95% Confidence Limits
% -- %
s
t
a
e
n
d
0.00
Treatment 2
e
n
d
e
n
d
Mortality
start/end
% control orgs
producing 3rd
brood
92%
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
cq _______ ___
ator in Responsible Charge
ofvLabdratory Supervisor
D.O.
Control
t
1st sample
s
t
a
r
t
1st sample
s
t
a
r
t
2nd sample
|
Note: Please
Complete This
Section Also
Effluent %: 90%
TREATMENT 2 ORGANISMS
Laboratory Pe
Siqhatuth c
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
Complete This For Either Test
Test Start Date: 05/22/02
Collection (Start) Date
Sample 1: 05/20/02 :
Sample Type/Duration
I Organism Tested:
Copied from DWQ form AT-1 (3/87)
Work Order:
Chronic Test Results
Calculated t = -0.188
Tabular t = 2.508
% Reduction = -0.88
X__/ '
“W * PASSED:
rev. 11/95 (DUBIA ver. 4.41)