NC0025453_Historical information_20121231 (2)

LEGEND
EFFLUENT FORCE MAIN
EFFLUENT OUTFALL
INFLUENT SEWER
LITTLE CREEK WRF
USGS QUAD: CLAYTON, NC 2002
; 70
SCALE: 1 "=2000'
/.CLAYTON • .
EFFLUENT
OUTFALL
RECEIVING STREAM: NEUSE RIVER
RIVER BASIN: NEUSE RIVER BASIN
EFFLUENT
FORCE MAIN
DISCHARGE
POINT 001
LAT. 35*39' 50"
LONG. 78*25' 26"
EXISTING 18"
INF. SEWER
EXISTING 24"
INF. SEWER
Ct
Ct
LITTLE CREEK
WATER RECLAMATION
FACILITY
TOWN OF CLAYTON
LITTLE CREEK WATER
RECLAMATION FACILITY
NPDES PERMIT NO. NC0025453
-----------TW3-
5
* pl s >
J
■
I
I
34o
V
ift
■
1 I /I
Z.. ■'
- ’ J.. \
/ i.
h
THE WOOTEN COMPANY
_____'.-I.
r
■
Bi
\ I J ’A:
U’’ j
V / r
/ a
-ss—Ct*
cpi S T z
• Av V' <. \
=“'; =
•L' V1'': £
Legend
Wastewater / Effluent
— Sludge
Decant / Filter Backwash / Bed Underdrainage
Scum
[
(2.5 mgd)
Anaerobic Selector Tank
r
z
ENGINEERINGI
o PLANNING
I
ARCHITECTUREClerigester
1
(3.026 mgd)
(5.0 tngd)p.s.tfX.Dist. Box (1.67 mgd)
M -----<
Ox. Basin No. 1' (1.974 mgd)
Filter BackwashP.S. I
I ►
►
—I 1IFirst Stage Anoxic Tank Drain□l
5
ISludge Recirc.Waste SludgeInfluent
~1
jtf.p.sInfluent
II a
(2.5 mgd)iZTT J(2.5 mgd)O-
(2.5 mgd)
Eff. Pump Sta.Decant
EXHIBIT 1
Schematic Flow Diagram of Clayton LCWRF
P.S.
I
!
I
□
P.S. '
— —
- - c- -
/
I
I I
I .
Clar. Dist. Box
Scum
Sludge
Drying Beds
Secondary
Clarifier-^.
I
/—First Stage Anoxic Tank
/—Ox. Basin No. 2
Trickling
Filter -
Stabilization/
Storage Tank—
Sludge
Drying Beds
Sludge
Thickening Bldg
UVDisinf/
Chlorination -
Dechlorination
Flow Balance at
Design Average Daily Flow
Clarifier
No. 3
Tertiary
Filters
THE
WOOTEN
COMPANY
- Influent
Pump Sta.
Clarifier
No. 1 -7
1
3
To Neuse River
/1
al—
Chemical
Stor. Area-
\
Sludge
Stabilization/
Storage Tank—
I
Sludge
Loading
Sta.-----
Existing Process Train
is Abandoned
"I
A
□ □.B.
(0.83 mgd)
k^5 mgd)
Chem Feed
& Sludge
Thick. Bldg.
r. • *Screening
& Grit Rem.
Aerobic
Digester
/''/Clarifier
4 No. 2—z
I
I ’ ” ’
(1.67 mgd) ■
LEGEND
PROCESS FLOW
RAS FLOW
WAS FLOW
WOOTENPIPE
COMPANYWATER PIPE
STRUCTURE
ENGINEERING
FENCE
PLANNING
TREE LINE
ACCESS ROAD
O«H
AERATION BASIN NO 2
—^uu •
SLUDGE
o
EXHIBIT 2
PROCESS FLOW DIAGRAM OF CLAYTON LCWRF
AEROBIC
DIGESTER
SECONDARY
CLARIFIERNO.1
BLOWER
ASSEUBLV
ANAEROBIC
SELECTOR
TANKS
SECONDARY
CLARIFIER NO 3
EX. 6‘
WATER LINE
12- DI
FORCE MAIN -
TERTIARY
FILTERS
HEADWORKS
EX. INFLUENT
PUMP STATION
-SLUDGE
DRAWOFF
VAULT
SLUDGE
[STABILIZATION IHOLDINGI
TANK No 1 /
SLUDGE
STABILIZATION/HOLDING
i TANK No 2 I
ANOXIC
TANKS
DRYIN 3
BEDS
a
//
October 3, 2012
9 20)2005
Dear Mr. Biggs:
Sincerely,
1
R. STEVEN BIGGS, TOWN MANAGER
TOWN OF CLAYTON
TO BOX 879
CLAYTON, NC 27520
Beverly Eaves Perdue
Governor
Lizette Davis-Houston
Point Source Branch
Dee Freeman
Secretary
1617 Mail Service Center, Raleigh, North Carolina 27699-1617
Location: 512 N. Salisbury St. Raleigh, North Carolina 27604
Phone: [phone] \ FAX: [phone]
Internet: www.ncwaterquality.org
An Equal Opportunity \ Affirmative Action Employer
If you have any additional questions concerning renewal of the subject permit, please contact Joe Corporon
at [phone].
Subject: Receipt of permit renewal application
NPDES Permit NC0025453
Little Creek Water Reclamation Facility
Wake County
The NPDES Unit received your permit renewal application on October 2, 2012. A member of the NPDES
Unit will review your application. They will contact you if additional information is required to complete your
permit renewal. You should expect to receive a draft permit approximately 30-45 days before your existing permit
expires.
u Ke -
^TOne, NorthCarolina
Naturally
NCDENR
North Carolina Department of Environment and Natural Resources
Division of Water Quality
> Charles Wakild, P.E.
Director
cc: CENTRAL FILES
Raleigh Regional Office/Surface Water Protection
NPDES Unit
Town of Clayton, North Carolina
September 2012
License No. F-0115
Application for Renewal of
NPDES Permit No. NC0025453
«s
“SERVICE”“ENVIRONMENT"
September 21, 2012
Re:
Dear Mr: Belnick
Sincen
Enclosures
653 Highway 42 West • P.O. Box 879 • Clayton, North Carolina 27520 • [phone] • Fax [phone]
VEHICLE MAINTENANCE
[phone]
R.Steven Biggs
Town Manager
ELECTRIC SERVICE
[phone]
WATER RECLAMATION
[phone]
PUBLIC WORKS
[phone]
1.
2.
3.
4.
5.
6.
7.
Mr. Tom Belnick, Supervisor
Division of Water Quality
Surface Water Protection Section - NPDES
1617 Mail Service Center
Raleigh, NC 27699-1617
TOWN OF CLAYTON
OPERATIONS CENTER
Request for Permit Renewal
NPDES Permit No. NC0025453
Town of Clayton, North Carolina
Enclosed, please find one (1) original and two (2) copies of an application package requesting
renewal of the NPDES Permit for the Little Creek Water Reclamation Facility (LCWRF) located in
Clayton. North Carolina. Each application package contains the following:
EPA NPDES Form 2A
Topographic Site Map
Schematic Flow Diagram
Process Flow Diagram
Three (3) Priority Pollutant Analyses
Four (4) Chronic Toxicity Tests
Sludge Management Plan
If you have any questions or require any additional information, please do not hesitate to contact me
at 919-553-5002.
NPDES FORM 2A APPLICATION OVERVIEW
APPLICATION OVERVIEW
BASIC APPLICATION INFORMATION:
A.
B.
C.Certification. All applicants must complete Part C (Certification).
SUPPLEMENTAL APPLICATION INFORMATION:
D.
1.
2.
3.
E.
1.
2.
3.
F.
1.
2.
a.
b.
c.
G.
ALL APPLICANTS MUST COMPLETE PART C (CERTIFICATION)
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 1 of 23
Basic Application Information for all Applicants. All applicants must complete questions A.1 through A.8. A treatment works
that discharges effluent to surface waters of the United States must also answer questions A.9 through A. 12.
Additional Application Information for Applicants with a Design Flow > 0.1 mgd. All treatment works that have design flows
greater than or equal to 0.1 million gallons per day must complete questions B. 1 through B.6.
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
FORM
2A
NPDES
Toxicity Testing Data. A treatment works that meets one or more of the following criteria must complete Part E (Toxicity Testing
Data):
Has a design flow rate greater than or equal to 1 mgd,
Is required to have a pretreatment program (or has one in place), or
Is otherwise required by the permitting authority to submit results of toxicity testing.
Industrial User Discharges and RCRA/CERCLA Wastes. A treatment works that accepts process wastewater from any
significant industrial users (SIUs) or receives RCRA or CERCLA wastes must complete Part F (Industrial User Discharges
and RCRA/CERCLA Wastes). SIUs are defined as:
All industrial users subject to Categorical Pretreatment Standards under 40 Code of Federal Regulations (CFR) 403.6 and
40 CFR Chapter I, Subchapter N (see instructions); and
Any other industrial user that:
Discharges an average of 25,000 gallons per day or more of process wastewater to the treatment works (with certain
exclusions); or
Contributes a process wastestream that makes up 5 percent or more of the average dry weather hydraulic or organic
capacity of the treatment plant; or
Is designated as an SIU by the control authority.
Expanded Effluent Testing Data. A treatment works that discharges effluent to surface waters of the United States and meets
one or more of the following criteria must complete Part D (Expanded Effluent Testing Data):
Has a design flow rate greater than or equal to 1 mgd,
Is required to have a pretreatment program (or has one in place), or
Is otherwise required by the permitting authority to provide the information.
Form 2A has been developed in a modular format and consists of a “Basic Application Information” packet
and a “Supplemental Application Information" packet. The Basic Application Information packet is divided
into two parts. All applicants must complete Parts A and C. Applicants with a design flow greater than or
equal to 0.1 mgd must also complete Part B. Some applicants must also complete the Supplemental
Application Information packet. The following items explain which parts of Form 2A you must complete.
Combined Sewer Systems. A treatment works that has a combined sewer system must complete Part G (Combined Sewer
Systems).
BASIC APPLICATION INFORMATION
PART A. BASIC APPLICATION INFORMATION FOR ALL APPLICANTS:
All treatment works must complete questions A.1 through A.8 of this Basic Application Information Packet.
A.1. Facility Information.
Facility Name Town of Clayton - Little Creek Water Reclamation Facility
Mailing Address Post Office Box 879
Clayton. North Carolina 27520
Contact Person Steve Biggs
Title Town Manager
Telephone Number [phone]
Facility Address 1000 Durham Street Extension
(not P.O. Box)Clayton, North Carolina
A.2. Applicant Information. If the applicant is different from the above, provide the following:
Applicant Name Same as above
Mailing Address
Contact Person
Title
Telephone Number ()
Is the applicant the owner or operator (or both) of the treatment works?
IZ1 owner operator
Indicate whether correspondence regarding this permit should be directed to the facility or the applicant.
□ facility E applicant
NPDES NC0025453 PSD
UIC Other WQ0022224
RCRA Other
A.4. Collection System Information. Provide information on municipalities and areas served by the facility. Provide the name and population of each
entity and, if known, provide information on the type of collection system (combined vs. separate) and its ownership (municipal, private, etc.).
Name Population Served Type of Collection System Ownership
Town of Clayton 13,700 Separate Municipal
East Clayton Industrial Area Industrial Separate Municipal
Total population served 13.700
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 2 of 23
A.3. Existing Environmental Permits. Provide the permit number of any existing environmental permits that have been issued to the treatment works
(include state-issued permits).
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
-■ OCT 0 2 2o;2 i I
A.5.Indian Country.
Is the treatment works located in Indian Country?a.
Yes 0 No
b.
Yes £3 No
A.6.
Design flow rate Z5.a.mgd
Two Years Ago Last Year This Year
b.Annual average daily flow rate 1.727 mgd 1.56 mgd 1.602 mgd
Maximum daily flow ratec.4.366 mgd 2.524 mgd 2.782 mgd
A.7.Collection System. Indicate the type(s) of collection system(s) used by the treatment plant. Check all that apply. Also estimate the percent
100 %
%
A.8.Discharges and Other Disposal Methods.
Does the treatment works discharge effluent to waters of the U.S.?a.0 Yes No
If yes, list how many of each of the following types of discharge points the treatment works uses:
i.Discharges of treated effluent 1
ii.Discharges of untreated or partially treated effluent 0
iii.Combined sewer overflow points 0
Constructed emergency overflows (prior to the headworks)iv.0
Otherv.0
b.
that do not have outlets for discharge to waters of the U.S.?E No
If yes, provide the following for each surface impoundment:
Location:
mgd
Does the treatment works land-apply treated wastewater?c.E Yes No
If yes, provide the following for each land application site:
Location:Pine Hollow Golf Course - Reclaimed Water
Number of acres:97.4 (approximately)
0.23784 mgd
E2 intermittent?
d.
Yes S No
EPA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 3 of 23
contribution (by miles) of each.
El Separate sanitary sewer
Does the treatment works discharge or transport treated or untreated wastewater to another
treatment works?
Annual average daily volume applied to site:
Is land application continuous or
Annual average daily volume discharge to surface impoundment(s)
Is discharge continuous or intermittent?
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
Combined storm and sanitary sewer
Does the treatment works discharge effluent to basins, ponds, or other surface impoundments
..............................„................................. ... 1 Yes
Does the treatment works discharge to a receiving water that is either in Indian Country or that is upstream from (and eventually flows
through) Indian Country?
Flow. Indicate the design flow rate of the treatment plant (i.e., the wastewater flow rate that the plant was built to handle). Also provide the
average daily flow rate and maximum daily flow rate for each of the last three years. Each year's data must be based on a 12-month time period
with the 12 month of “this year" occurring no more than three months prior to this application submittal.
If transport is by a party other than the applicant, provide:
Transporter Name
Mailing Address
Contact Person
Title
Telephone Number (1
For each treatment works that receives this discharge, provide the following:
Name
Mailing Address
Contact Person
Title
Telephone Number £
If known, provide the NPDES permit number of the treatment works that receives this discharge
Provide the average daily flow rate from the treatment works into the receiving facility. mgd
e.
□ Yes E No
If yes, provide the following for each disposal method:
Description of method (including location and size of site(s) if applicable):
Is disposal through this method □ intermittent?or
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 4 of 23
Annual daily volume disposed by this method:
□ continuous
Does the treatment works discharge or dispose of its wastewater in a manner not included
in A.8. through A.8.d above (e.g., underground percolation, well injection):
If yes. describe the mean(s) by which the wastewater from the treatment works is discharged or transported to the other treatment works
(e.g.. tank truck, pipe).
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
WASTEWATER DISCHARGES:
A.9. Description of Outfall.
Outfall number 001a.
b.Location
Distance from shore (if applicable) c.ft.
d.Depth below surface (if applicable)3 ft.
Average daily flow ratee.1.602 mgd “(2012 Data)**
f.Does this outfall have either an intermittent or a periodic discharge? Yes S No (gotoA.9.g.)
If yes, provide the following information:
Number f times per year discharge occurs:
Average duration of each discharge:
Average flow per discharge: mgd
Months in which discharge occurs:
Is outfall equipped with a diffuser? Yes 0 Nog-
J
A.10. Description of Receiving Waters.
Name of receiving watera.Neuse River
b.Name of watershed (if known)Neuse Subbasin 03-04-02
United States Soil Conservation Service 14-digit watershed code (if known):
Name of State Management/River Basin (if known): Neuse River Basinc.
United States Geological Survey 8-digit hydrologic cataloging unit code (if known):03020201
d.Critical low flow of receiving stream (if applicable) 7Q10 = 186 cfs on USGS data (summer 7Q10)
acute cfs chronic cfs
Total hardness of receiving stream at critical low flow (if applicable): e.mg/l of CaCO3
EPA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 5 of 23
Town of Clayton - Neuse River Between SR 1700 and NC Hwy 42
(City or town, if applicable)
78 25’26 •
(Longitude)
27527
(Zip Code)
North Carolina
(State)
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
Johnston
(County)
353950"
(Latitude)
If you answered “Yes” to question A.8.a. complete questions A.9 through A.12 once for each outfall (including bypass points) through
which effluent is discharged. Do not include information on combined sewer overflows in this section. If you answered “No" to question
A. Q-3- g° to Part B. ‘Additional Application Information for Applicants with a Design Flow Greater than or Equal to 0.1 mgd.”
A.11. Description of Treatment
a.
Describe:
b.
Design BODS removal or Design CBOD5 removal 98 %
Design SS removal 96 %
Design P removal 75 %
Design N removal 92.5 %
Other %
What type of disinfection is used for the effluent from this outfall? If disinfection varies by season, please describe:c.
UV Disinfection
If disinfection is by chlorination is dechlorination used for this outfall? Yes No
Does the treatment plant have post aeration? Yes No
Outfall number:001
MAXIMUM DAILY VALUE AVERAGE DAILY VALUEPARAMETER
Value Units Value Units Number of Samples
6.8 s.u.
8.2 s.u.
Flow Rate 4.366 mgd 1.63 mgd 911
Temperature (Winter)22 C15.7 392
°C33 C24 519
AVERAGE DAILY DISCHARGE
POLLUTANT ML/MDL
Cone.Units Cone.Units
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
BODS 15.4 mg/l 2.9 mg/l 624
CBOD5 N/A N/A N/A N/A N/A
FECAL COLIFORM #/100 mL5800 10.3 624#/100 mL
TOTAL SUSPENDED SOLIDS (TSS)28 mg/l 2.46 mg/l 624
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 6 of 23
pH (Minimum)
pH (Maximum)
END OF PART A.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
What level of treatment are provided? Check all that apply.
Primary Secondary
0 Advanced Other.
Indicate the following removal rates (as applicable):
PERMIT ACTION REQUESTED:
Renewal
Number of
Samples
ANALYTICAL
METHOD
RIVER BASIN:
Neuse
BIOCHEMICAL OXYGEN
DEMAND (Report one)
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
Temperature (Summer)
_________' For pH please report a minimum and a maximum daily value
MAXIMUM DAILY
DISCHARGE
“C
A.12. Effluent Testing Information. All Applicants that discharge to waters of the US must provide effluent testing data for the following
parameters. Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is
discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data
collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of
40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a
minimum, effluent testing data must be based on at least three samples and must be no more than four and one-half years apart.
BASIC APPLICATION INFORMATION
PART B.
B.l.
a.
b.
c.
e.
f.
Mailing Address:
Telephone Number:L )
Responsibilities of Contractor:
a.
b.
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 7 of 23
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility.
NC0025453
Indicate whether the planned improvements or implementation schedule are required by local, State, or Federal agencies.
Yes No
ADDITIONAL APPLICATION INFORMATION FOR APPLICANTS WITH A DESIGN FLOW GREATER THAN OR
EQUAL TO 0.1 MGD (100,000 gallons per day).
All applicants with a design flow rate > 0,1 mgd must answer questions B.l through B.6. All others go to Part C (Certification).
Inflow and Infiltration. Estimate the average number of gallons per day that flow into the treatment works from inflow and/or infiltration.
550,000 (estimated) gpd
Briefly explain any steps underway or planned to minimize inflow and infiltration.
The Town of Clayton has an ongoing sewer system evaluation and rehabilitation program.________________________
B.5. Scheduled improvements and Schedules of Implementation. Provide information on any uncompleted implementation schedule or
uncompleted plans for improvements that will affect the wastewater treatment, effluent quality, or design capacity of the treatment works. If the
treatment works has several different implementation schedules or is planning several improvements, submit separate responses to question B.5
for each. (If none, go to question B.6.)
List the outfall number (assigned in question A.9) for each outfall that is covered by this implementation schedule.
B.2. Topographic Map. Attach to this application a topographic map of the area extending at least one mile beyond facility property boundaries. This
map must show the outline of the facility and the following information. (You may submit more than one map if one map does not show the entire
area.)
The area surrounding the treatment plant, including all unit processes.
The major pipes or other structures through which wastewater enters the treatment works and the pipes or other structures through which
treated wastewater is discharged from the treatment plant. Include outfalls from bypass piping, if applicable.
Each well where wastewater from the treatment plant is injected underground.
d. Wells, springs, other surface water bodies, and drinking water wells that are: 1) within ’A mile of the property boundaries of the treatment
works, and 2) listed in public record or otherwise known to the applicant.
Any areas where the sewage sludge produced by the treatment works is stored, treated, or disposed.
If the treatment works receives waste that is classified as hazardous under the Resource Conservation and Recovery Act (RCRA) by truck, rail,
or special pipe, show on the map where the hazardous waste enters the treatment works and where it is treated, stored, and/or disposed.
B.3. Process Flow Diagram or Schematic. Provide a diagram showing the processes of the treatment plant, including all bypass piping and all
backup power sources or redunancy in the system. Also provide a water balance showing all treatment units, including disinfection (e.g.,
chlorination and dechlorination). The water balance must show daily average flow rates at influent and discharge points and approximate daily flow
rates between treatment units. Include a brief narrative description of the diagram.
B.4. Operation/Maintenance Performed by Contractor(s).
Are any operational or maintenance aspects (related to wastewater treatment and effluent quality) of the treatment works the responsibility of a
contractor? Yes £3 No
If yes, list the name, address, telephone number, and status of each contractor and describe the contractor's responsibilities (attach additional
pages if necessary).
Name:
RIVER BASIN:
Neuse
If the answer to B.5.b is “Yes.” briefly describe, includingc.
d.
applicable. Indicate dates as accurately as possible.
Schedule Actual Completion
Implementation Stage MM/DD/YYYY MM/DD/YYYY
- Begin Construction /
- End Construction I
■ Begin Discharge
- Attain Operational Level
Have appropriate permits/clearances concerning other Federal/State requirements been obtained?e.□ Yes □ No
Describe briefly:
B.6.
Outfall Number: 001
AVERAGE DAILY DISCHARGE
POLLUTANT MUMDL
Cone.Units Cone.Units
AMMONIA (as N)mg/1 0.49 mg/l 624 ERA 350.1 0.5 mg/l
N/A N/A
DISSOLVED OXYGEN 9.90 mg/l 8.17 mg/l 624
3.36 mg/l 1.53 mg/l 149 ERA 351.2 0.25 mg/l
9.24 mg/l 2.97 mg/l 148 ERA 353.2 0.02 mg/l
OIL and GREASE 5 mg/l <5 mg/l 3 ERA1664A 5 mg/l
PHOSPHORUS (Total)4.1 mg/l 1.02 mg/l 138 ERA 365.4 0.1 mg/l
878 mg/l 681 mg/l 3 SM 2540B 1 mg/l
OTHER
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 8 of 23
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
7.28
TOTAL DISSOLVED SOLIDS
(TDS)
TOTAL KJELDAHL
NITROGEN (TKN)
CHLORINE (TOTAL
RESIDUAL, TRC)
NITRATE PLUS NITRITE
NITROGEN
END OF PART B.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
MAXIMUM DAILY
DISCHARGE
PERMIT ACTION REQUESTED:
Renewal
Number of
Samples
ANALYTICAL
METHOD
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
new maximum daily inflow rate (if applicable).
EFFLUENT TESTING DATA (GREATER THAN 0.1 MGD ONLY).
Applicants that discharge to waters of the US must provide effluent testing data for the following parameters. Provide the indicated
effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include information
on combine sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate
QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a minimum effluent testing data must be
based on at least three pollutant scans and must be no more than four and on-half years old.
Provide dates imposed by any compliance schedule or any actual dates of completion for the implementation steps listed below, as
applicable. For improvements planned independently of local, State, or Federal agencies, indicate planned or actual completion dates, as
BASIC APPLICATION INFORMATION
PARTC. CERTIFICATION
ALL APPLICANTS MUST COMPLETE THE FOLLOWING CERTIFICATION.
Name and official title
Signature
Telephone number
Date signed
works or identify appropriate permitting requirements.
SEND COMPLETED FORMS TO:
27699-1617
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 9 of 23
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
NCDENR/ DWQ
Attn: NPDESUnit
1617 Mail Service Center
Raleigh, North Carolina
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
R. Steven Biggs, Town Manager
[phone]____________________________________________________________
/ oZ / /cjo I
Upon request of the permitting authority, you must submit any other information necessary to assure wastewater treatment practices at the treatment
All applicants must complete the Certification Section. Refer to instructions to determine who is an officer for the purposes of this
certification. All applicants must complete all applicable sections of Form 2A, as explained in the Application Overview. Indicate below which
parts of Form 2A you have completed and are submitting. By signing this certification statement, applicants confirm that they have reviewed
Form 2A and have completed all sections that apply to the facility for which this application is submitted.
Indicate which parts of Form 2A you have completed and are submitting:
Lj Basic Application Information packet Supplemental Application Information packet:
0 Part D (Expanded Effluent Testing Data)
E Part E (Toxicity Testing: Biomonitoring Data)
S Part F (Industrial User Discharges and RCRA/CERCLA Wastes)
□ Part G (Combined Sewer Systems)
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system
designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who
manage the system or those persons directly responsible for gathering the information, the information is, to the best of my knowledge and belief, true,
accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment
for knowing violations.
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:RIVER BASIN:
Renewal Neuse
SUPPLEMENTAL APPLICATION INFORMATION
PART D. EXPANDED EFFLUENT TESTING DATA
Outfall number:001
AVERAGE DAILY DISCHARGE
POLLUTANT ML/MDLCone.Units Mass Units Cone.Units Mass Units
METALS (TOTAL RECOVERABLE). CYANIDE. PHENOLS, AND HARDNESS.
ANTIMONY < 3 ug/l <3 ug/l 3 EPA 200.8 3
ARSENIC < 5 ug/l < 5 ug/l 3 5
BERYLLIUM < 2 ug/l < 2 ug/l 3 2
CADMIUM < 2 ug/l < 2 ug/l 3 EPA 200.8 2
CHROMIUM < 5 ug/l <5 ug/l 3 EPA 200.7 5
COPPER 7.31 ug/l 7.14 ug/l 3 EPA 200.7 2
LEAD < 5 ug/l < 5 ug/l SM3113B3 5
MERCURY < 0.2 ug/l ug/l< 0.2 3 EPA 245.1 0.2
NICKEL < 10 ug/l ug/l< 10 3 EPA 200.7 10
SELENIUM < 10 ug/l < 10 ug/l 3 SM 3113B 10
SILVER ug/l< 5 ug/l< 5 3 EPA 200.7 5
THALLIUM ug/l< 1 ug/l< 1 3 EPA 200.8 1
ZINC 144 ug/l 78.8 ug/l 3 SM 3113B 10
CYANIDE < .005 mg/l < .005 mg/l 3 SM 4500CN-E .005
7 ug/l < .005 ug/l 3 SM 510 A&B .005
HARDNESS (as CaCO3)73 mg/l 60.7 mg/l 3 SM 2340C 2
Use this space (or a separate sheet) to provide information on other metals requested by the permit writer
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 10 of 23
TOTAL PHENOLIC
COMPOUNDS
Number
of
Samples
ANALYTICAL
METHOD
EPA 200.8/
SM3113B
EPA 200.7/
EPA 200.8
Little Creek Water Reclamation Facility,
NC0025453
(Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE
Refer to the directions on the cover page to determine whether this section applies to the treatment works.
Effluent Testing: 1.0 mgd and Pretreatment Works. If the treatment works has a design flow greater than or equal to 1.0 mgd or it has (or is required
to have) a pretreatment program, or is otherwise required by the permitting authority to provide the data, then provide effluent testing data for the following
pollutants. Provide the indicated effluent testing information and any other information required by the permitting authority for each outfall through which
effluent is discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected
through analyses conducted using 40 CFR Part 136 methods. In addition, these data must comply with QA/QC requirements of 40 CFR Part 136 and
other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. Indicate in the blank rows provided below
any data you may have on pollutants not specifically listed in this form. At a minimum, effluent testing data must be based on at least three pollutant
scans and must be no more than four and one-half years old.
Note: A minimum of 3 pollutant scans were prepared for the below constituents. For some constituents the detection limit decreased from the
previous scan. For such cases, the most recent data from 6/7/12 is utilized corresponding with the lower detection limit. See attached analytical
results.
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:RIVER BASIN:
Renewal Neuse
Outfall number: 001
AVERAGE DAILY DISCHARGE
POLLUTANT MUMDLCone.Units Mass Units Cone.Units Mass Units
VOLATILE ORGANIC COMPOUNDS
ACROLEIN < 5 ug/l < 5 ug/l 3 EPA 624 5
ACRYLONITRILE < 5 ug/l < 5 ug/l 3 EPA 624 5
BENZENE < 5 ug/l I < 5 ug/l 3 EPA 624 5
BROMOFORM < 5 ug/l ug/l< 5 3 EPA 624 5
< 5 ug/l < 5 ug/l 3 EPA 624 5
CHLOROBENZENE < 5 ug/l < 5 ug/l 3 EPA 624 5
< 5 ug/l ug/l< 5 3 EPA 624 5
CHLOROETHANE < 5 ug/l < 5 ug/l 3 EPA 624 5
< 5 ug/l < 5 ug/l 3 EPA 624 5
CHLOROFORM < 5 ug/l <5 ug/l 3 EPA 624 5
ug/l< 5 < 5 ug/l 3 EPA 624 5
1,1-DICHLOROETHANE < 5 ug/l < 5 ug/l 3 EPA 624 5
1.2-DICHLOROETHANE < 5 ug/l < 5 ug/l 3 EPA 624 5
ug/l< 5 < 5 ug/l 3 EPA 624 5
< 5 ug/l < 5 ug/l 3 EPA 624 5
1,2-DICHLOROPROPANE < 5 ug/l ug/l< 5 3 EPA 624 5
ug/l< 5 < 5 ug/l EPA 6243 5
ETHYLBENZENE < 5 ug/l ug/l< 5 3 EPA 624 5
METHYL BROMIDE < 10 ug/l < 10 ug/l 3 EPA 624 10
METHYL CHLORIDE < 5 ug/l < 5 ug/l 3 EPA 624 5
METHYLENE CHLORIDE < 5 ug/l < 5 ug/l 3 EPA 624 5
< 5 ug/l ug/l< 5 3 EPA 624 5
ug/l< 5 < 5 ug/l 3 EPA 624 5
TOLUENE < 5 ug/l < 5 ug/l 3 EPA 624 5
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 11 of 23
Number
of
Samples
Little Creek Water Reclamation Facility,
NC0025453
ANALYTICAL
METHOD
TETRACHLORO­
ETHYLENE
TRANS-1.2-DICHLORO-
ETHYLENE
1,1,2.2-TETRA-
CHLOROETHANE
1,3-DICHLORO-
PROPYLENE
CARBON
TETRACHLORIDE
CHLORODIBROMO­
METHANE
2-CHLOROETHYLVINYL
ETHER
DICHLOROBROMO­
METHANE
1,1-DICHLORO-
ETHYLENE
(Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:RIVER BASIN:
Renewal Neuse
Outfall number: 001
AVERAGE DAILY DISCHARGE
POLLUTANT ML/MDLCone.Units Mass Units Cone.Units Mass Units
< 5 ug/l < 5 ug/l 3 EPA 624 5
< 5 ug/l < 5 ug/l 3 EPA 624 5
TRICHLOROETHYLENE ug/l< 5 < 5 ug/l 3 EPA 624 5
VINYL CHLORIDE < 5 ug/l < 5 ug/l 3 EPA 624 5
Use this space (or a separate sheet) to provide information on other volatile organic compounds requested by the permit writer
ACID-EXTRACTABLE COMPOUNDS
P-CHLORO-M-CRESOL ug/l< 5 < 5 ug/l 3 EPA 625 5
2-CHLOROPHENOL ug/l< 5 ug/l< 5 3 EPA 625 5
2,4-DICHLOROPHENOL < 5 ug/l < 5 ug/l 3 EPA 625 5
2,4-DIMETHYLPHENOL < 10 ug/l < 10 ug/l 3 EPA 625 5
4,6-DINITRO-O-CRESOL < 20 ug/l < 20 ug/l 3 EPA 625 20
2.4-DINITROPHENOL < 50 ug/l < 50 ug/l 3 EPA 625 50
2-NITROPHENOL < 5 ug/l < 5 ug/l 3 EPA 625 5
4-NITROPHENOL < 50 ug/l < 50 ug/l 3 EPA 625 50
PENTACHLOROPHENOL < 25 ug/l < 25 ug/l 3 EPA 625 25
PHENOL < 5 ug/l < 5 ug/l 3 EPA 625 5
< 10 ug/l < 10 ug/l 3 EPA 625 10
Use this space (or a separate sheet) to provide information on other acid-extractable compounds requested by the permit writer
BASE-NEUTRAL COMPOUNDS
ACENAPHTHENE ug/l< 5 ug/l< 5 3 EPA 625 5
ACENAPHTHYLENE < 5 ug/l ug/l< 5 3 EPA 625 5
ANTHRACENE ug/l< 5 < 5 ug/l 3 EPA 625 5
BENZIDINE < 5 ug/l < 5 ug/l 3 EPA 625 5
BENZO(A)ANTHRACENE < 5 ug/l < 5 ug/l 3 EPA 625 5
BENZO(A)PYRENE < 5 ug/l < 5 ug/l 3 EPA 625 5
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 12 of 23
Number
of
Samples
ANALYTICAL
METHOD
2,4,6-
TRICHLOROPHENOL
1,1,2-
TRICHLOROETHANE
1.1,1-
TRICHLOROETHANE
Little Creek Water Reclamation Facility,
NC0025453
(Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:RIVER BASIN:
Renewal Neuse
Outfall number: 001
AVERAGE DAILY DISCHARGE
POLLUTANT ML/MDLCone.Units Mass Units Cone.Units Mass Units
< 5 ug/l < 5 ug/l 3 EPA 625 3
BENZO(GHI)PERYLENE < 5 ug/l < 5 ug/l 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
< 10 ug/l < 10 ug/l 3 EPA 625 10
< 5 ug/l < 5 ug/l 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
< 5 ug/l ug/l< 5 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
CHRYSENE ug/l< 5 < 5 ug/l 3 EPA 625 5
DI-N-BUTYL PHTHALATE < 5 ug/l < 5 ug/l 3 EPA 625 5
DI-N-OCTYL PHTHALATE < 5 ug/l < 5 ug/l 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
1.2-DICHLOROBENZENE < 5 ug/l < 5 ug/l 3 EPA 625 5
1.3-DICHLOROBENZENE < 5 ug/l < 5 ug/l 3 EPA 625 5
1.4-DICHLOROBENZENE < 5 ug/l < 5 ug/l EPA 6253 5
< 25 ug/l < 25 ug/l 3 EPA 625 5
DIETHYL PHTHALATE < 5 ug/l < 5 ug/l 3 EPA 625 5
DIMETHYL PHTHALATE < 5 ug/l < 5 ug/l 3 EPA 625 5
2,4-DINITROTOLUENE < 5 ug/l < 5 ug/l 3 EPA 625 5
2,6-DINITROTOLUENE < 5 ug/l < 5 ug/l 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 13 of 23
Number
of
Samples
Little Creek Water Reclamation Facility,
NC0025453
ANALYTICAL
METHOD
1,2-DIPHENYL-
HYDRAZINE
3,3-DICHLORO-
BENZIDINE
DIBENZO(A,H)
ANTHRACENE
4-CHLORPHENYL
PHENYL ETHER
2-CHLORO-
NAPHTHALENE
4-BROMOPHENYL
PHENYL ETHER
BUTYL BENZYL
PHTHALATE
BIS (2-ETHYLHEXYL)
PHTHALATE
BIS (2-CHLOROISO-
PROPYL) ETHER
BIS (2-CHLOROETHOXY)
METHANE
BIS (2-CHLOROETHYL)-
ETHER
3,4 BENZO­
FLUORANTHENE
BENZO(K)
FLUORANTHENE
(Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:RIVER BASIN:
Renewal Neuse
Outfall number. 001
AVERAGE DAILY DISCHARGE
POLLUTANT ML/MDLCone.Units Mass Units Cone.Units Mass Units
FLUORANTHENE < 5 ug/l < 5 ug/l 3 EPA 625 5
FLUORENE ug/l< 5 < 5 ug/l 3 EPA 625 5
HEXACHLOROBENZENE < 5 ug/l < 5 ug/l 3 EPA 625 5
< 5 ug/l <5 ug/l 3 EPA 625 5
< 10 ug/l < 10 ug/l 3 EPA 625 10
HEXACHLOROETHANE ug/l< 5 < 5 ug/l 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
ISOPHORONE < 10 ug/l < 10 ug/l 3 EPA 625 10
NAPHTHALENE ug/l< 5 < 5 ug/l 3 EPA 625 5
NITROBENZENE ug/l< 5 < 5 ug/l 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
<5 ug/l <5 ug/l 3 EPA 625 5
ug/l< 10 < 10 ug/l 3 EPA 625 10
PHENANTHRENE ug/l< 5 < 5 ug/l 3 EPA 625 5
PYRENE < 5 ug/l < 5 ug/l 3 EPA 625 5
< 5 ug/l < 5 ug/l 3 EPA 625 5
Use this space (or a separate sheet) to provide information on other base-neutral compounds requested by the permit writer
Use this space (or a separate sheet) to provide information on other pollutants (e.g., pesticides) requested by the permit writer
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 14 of 23
END OF PART D.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
Little Creek Water Reclamation Facility,
NC0025453
Number
of
Samples
ANALYTICAL
METHOD
N-NITROSODI-N-
PROPYLAMINE
N-NITROSODI-
METHYLAMINE
N-NITROSODI-
PHENYLAMINE
INDENO(1,2,3-CD)
PYRENE
HEXACHLORO­
BUTADIENE
HEXACHLOROCYCLO-
PENTADIENE
1,2,4-
TRICHLOROBENZENE
(Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE
SUPPLEMENTAL APPLICATION INFORMATION
PART E. TOXICITY TESTING DATA
E.l. Required Tests.
D acute Clayton submits quarterly toxicity testing results to NCDENR as required by their NPDES permit.
E.2.
Test number: Test number: Test number:
Test information.a.** SEE ATTACHED TOXICITY RESULTS**
Test Species & test method number
Age at initiation of test
Outfall number
Dates sample collected
Date test started
Duration
b. Give toxicity test methods followed.
Manual title
Edition number and year of publication
Page number(s)
Give the sample collection method(s) used. For multiple grab samples, indicate the number of grab samples used.c.
24-Hour composite
Grab
d.Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection
After dechlorination
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 15 of 23
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility, NC0025453
Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
0 chronic
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one
column per test (where each species constitutes a test). Copy this page if more than three tests are being reported.
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility s discharge points: 1) POTWs with a design flow rate greater than or equal to 1.0 mgd: 2) POTWs with a pretreatment program (or those that are
required to have one under 40 CFR Part 403); or 3) POTWs required by the permitting authority to submit data for these parameters.
• At a minimum, these results must include quarterly testing for a 12-month period within the past 1 year using multiple species (minimum of two
species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results
show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation, if one was conducted.
• If you have already submitted any of the information requested in Part E. you need not submit it again. Rather, provide the information
requested in question E.4 for previously submitted information. If EPA methods were not used, report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E.
If no biomonitoring data is required, do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
complete.
PERMIT ACTION REQUESTED:
Renewal
Test number:Test number:Test number:
Describe the point in the treatment process at which the sample was collected.e.
Sample was collected:
f.For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both
Chronic toxicity
Acute toxicity
g. Provide the type of test performed.
Static
Static-renewal
Flow-through
h.Source of dilution water. If laboratory water, specify type; if receiving water, specify source.
Laboratory water
Receiving water
i. Type of dilution water. If salt water, specify “natural" or type of artificial sea salts or brine used.
Fresh water
Salt water
Give the percentage effluent used for all concentrations in the test series.j-
k. Parameters measured during the test. (State whether parameter meets test method specifications)
pH
Salinity
Temperature
Ammonia
Dissolved oxygen
I.Test Results.
Acute:
%%%
LC50
95% C.l.%%%
Control percent survival %%%
Other (describe)
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 16 of 23
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility, NC0025453
Percent survival in 100%
effluent
RIVER BASIN:
Neuse
Chronic:
NOEC %%%
IC25 %%%
Control percent survival %%%
Other (describe)
m. Quality Control/Quality Assurance.
Is reference toxicant data available?
I I I I I I
Other (describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
Yes No If yes, describe:
E.4.
Date submitted:(MM/DD/YYYY)
Summary of results: (see instructions)
The Town of Clayton submits quarterly chronic toxicity tests to NCDENR in accordance with their NPDES permit.
Please see attached summary of the most recent test results.
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 17 of 23
Was reference toxicant test within
acceptable bounds?
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility, NC0025453
END OF PART E.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
What date was reference toxicant test
run (MM/DD/YYYY)?
Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information, or information regarding the
cause of toxicity, within the past four and one-half years, provide the dates the information was submitted to the permitting authority and a summary
of the results.
SUPPLEMENTAL APPLICATION INFORMATION
PART F. INDUSTRIAL USER DISCHARGES AND RCRA/CERCLA WASTES
F.l.
F.2.
Number of non-categorical SIUs.a.1
b.Number of CIUs,3
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU. If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU.
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
Name:Griffols
Mailing Address:8638 US Highway 70 West
Clayton, NC 27520
F.4.Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
Extraction Machines
Principal product(s):Biological Medical Products
Raw material(s):Blood Plasma
F.6.Flow Rate.
a.
262,500 (XX continuous or
b.
87,500 gpd (XX continuous or intermittent)
F.7.
E3 Yesa. No
b.Categorical pretreatment standards S Yes No
If subject to categorical pretreatment standards, which category and subcategory?
CFR 439.20
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 18 of 23
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
Pretreatment Standards. Indicate whether the SIU is subject to the following:
Local limits
Pretreatment program. Does the treatment works have, or is subject to, an approved pretreatment program?
0 Yes No
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
(Griffols’ permitted flow rate is 650,000 gpd)
Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent.
gpd (XX continuous or intermittent)
Non-process wastewater flow rate. Indicate the average daily volume of non-process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent.
Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works.
All treatment works receiving discharges from significant industrial users or which receive RCRA.CERCLA, or other remedial wastes must
complete part F.
GENERAL INFORMATION:
F.5. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU’s
discharge.
F.8.
Amount Units
E] No
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to origniate in
the next five years).
F.15. Waste Treatment.
a.
No
If yes, describe the treatment (provide information about the removal efficiency):
b.Is the discharge (or will the discharge be) continuous or intermittent?
Continuous Intermittent If intermittent, describe discharge schedule.
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 19 of 23
END OF PART F.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
Is this waste treated (or will be treated) prior to entering the treatment works?
Yes
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received). Include data on volume and concentration, if
known. (Attach additional sheets if necessary.)
F.12. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
Yes (complete F.13 through F.15.)
Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe?
Yes 0 No (go to F.12)
Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e.g..
upsets, interference) at the treatment works in the past three years?
Yes 0 No if yes. describe each episode.
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE:
F.9. RCRA Waste.
CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
Dedicated Pipe
F.11. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units).
EPA Hazardous Waste Number
F.10. Waste transport. Method by which RCRA waste is received (check all that apply):
Truck Rail
SUPPLEMENTAL APPLICATION INFORMATION
PART F. INDUSTRIAL USER DISCHARGES AND RCRA/CERCLA WASTES
F.l.
F.2.
Number of non-categorical SIUs.c.1
d.Number of CIUs.3
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU. If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU.
Name:Novo Nordisk Pharmaceutical Industries. Inc.
Mailing Address:3612 Powhattan Road
Clayton, NC 27520
F.4.
Mixing and Packaging
Principal product(s):Insulin for l.V. Injection
Raw material(s):Insulin Crystal
F.6.Flow Rate. (Novo’s permitted flow rate is 175,000 gpd)
c.
75.040 (XX continuous or
d.
36.960 gpd (XX continuous or intermittent)
F.7.
S Yesa. No
b.Categorical pretreatment standards 0 Yes No
If subject to categorical pretreatment standards, which category and subcategory?
CFR 439,40
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 20 of 23
Pretreatment Standards. Indicate whether the SIU is subject to the following:
Local limits
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
Pretreatment program. Does the treatment works have, or is subject to, an approved pretreatment program?
0 Yes No
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent.
gpd (XX continuous or intermittent)
Non-process wastewater flow rate. Indicate the average daily volume of non-process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent.
All treatment works receiving discharges from significant industrial users or which receive RCRA.CERCLA, or other remedial wastes must
complete part F.
GENERAL INFORMATION:
Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works.
Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
F.5. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
F.8.
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE:
F.9.RCRA Waste.
Amount Units
the next five years).
F.15. Waste Treatment.
c.
If yes, describe the treatment (provide information about the removal efficiency):
d.
Intermittent If intermittent, describe discharge schedule.
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 21 of 23
END OF PART F.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
Is the discharge (or will the discharge be) continuous or intermittent?
Continuous
Is this waste treated (or will be treated) prior to entering the treatment works?
Yes No
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received). Include data on volume and concentration, if
known. (Attach additional sheets if necessary.)
F.12. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
Yes (complete F. 13 through F.15.) 0 No
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to origniate in
Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe?
Yes E] No (go to F.12)
Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e.g.,
upsets, interference) at the treatment works in the past three years?
Yes 0 No If yes, describe each episode.
CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
F.10. Waste transport. Method by which RCRA waste is received (check all that apply):
Truck Rail Dedicated Pipe
F.11. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units).
EPA Hazardous Waste Number
SUPPLEMENTAL APPLICATION INFORMATION
PART F. INDUSTRIAL USER DISCHARGES AND RCRA/CERCLA WASTES
F.l.
F.2.
Number of non-categorical SIUs.e.1
f.Number of CIUs.3
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU. If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU.
Name:Hospira, Inc.
Mailing Address:8484 US Highway 70 West
Clayton, NC 27520
F.4.Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
Mixing and Packaging
F.5.
Principal product(s):I.V. Nutritional Products
Raw material(s):Soybean Oil. Egg Yolks. Glycerin. Amino Acids
F.6.Flow Rate.(Hospira's permitted flow rate is 160,000 gpd)
e.
46,200 gpd (XX continuous or
f.
30.800 gpd (XX continuous or intermittent)
F.7.Pretreatment Standards. Indicate whether the SIU is subject to the following:
Local limits S Yes Noa.
b.Categorical pretreatment standards Yes No
If subject to categorical pretreatment standards, which category and subcategory?
CFR 439.40
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 22 of 23
Pretreatment program. Does the treatment works have, or is subject to, an approved pretreatment program?
S Yes No
Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent.
intermittent)
Non-process wastewater flow rate. Indicate the average daily volume of non-process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent.
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
All treatment works receiving discharges from significant industrial users or which receive RCRA.CERCLA, or other remedial wastes must
complete part F.
GENERAL INFORMATION: . / U - , . v
Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works.
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
F.8.
EPA Hazardous Waste Number Units
the next five years).
F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received). Include data on volume and concentration, if
known. (Attach additional sheets if necessary.)
F.15. Waste Treatment.
e.
If yes, describe the treatment (provide information about the removal efficiency):
f.Is the discharge (or will the discharge be) continuous or intermittent?
Continuous Intermittent If intermittent, describe discharge schedule.
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 23 of 23
END OF PART F.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
Is this waste treated (or will be treated) prior to entering the treatment works?
Yes No
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
F.12. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
Yes (complete F.13 through F.15.) No
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to origniate in
Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e g.,
upsets, interference) at the treatment works in the past three years?
Yes 0 No If yes, describe each episode.
CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
F.10. Waste transport. Method by which RCRA waste is received (check all that apply):
Truck Rail Dedicated Pipe
F.11. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units).
Amount
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK. RAIL. OR DEDICATED PIPELINE:
F.9. RCRA Waste. Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe?
Yes 0 No (go to F.12)
Topographic Map
Schematic Flow Diagram
Process Flow Diagram
Priority Pollutant Analyses (PPAs)
10
Page 1
Quantitation
Level
/Antimony
Arsenic
Beryllium
Cadmium
Chromium
Copper
Lead
Grab
Grab
Grab
Grab
Grab
EPA 200,8
SM3.U3B
EPA200.7
SM3113B
EPA200.7
EPA200.7
SM3113B
EPA245.1
EPA200.7
SM3113B
EPA200.7
EPA2008
SM31 13B
EPA353.2
EPA351.2
EPA365.4
SM2540B
SM2340C
HACK 10014
EPA 1664
Analytical i
Method
EPA350 1
<5 ug/1
<5 ug/1
<5 ug/1
<5 ug/1
<5 ug/1
<10 ug/1
<5 ug/1
<5 ug/1
<10 ug/1
<5 ug/1
<5 ug/1
<5 ug/1
<5 ug/1
<5 ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
Ug/1
mg/1
mg/1
ug/1
ug/1
ug/1
Acrolein
Acrylonitrile
Benzene
Bromoform
Carbon tetrachloride
Chlorobenzene
Chlorodibromomcthanc
Chlorocthane
2-chloroethylvinyl ether
Chloroform
Dichlorobromomethanc
1.1- dichloroethane
1.2- dichloroethane
Trans-1,2-dichloroethylene
Mercury
Nickel
Selenium
Silver
Thallium
Zinc
Cyanide
Total phenolic compounds
Parameter
Ammonia (as N)______
Dissolved oxygen
Nitrate/Nitrite
Total Kjeldahl nitrogen
Total Phosphorus
Total dissolved solids
Hardness
Chlorine (total residual, TRC)
Oil and grease
-p-
Sample Type
Composite
Grab
Composite
Composite
Composite
Composite
Composite
5/29/2012 Grab
Grab
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
2
5
2
10
0.2
10
10
5
Units of
Measure
ment
■ng/!
mg/1
mg/1
mg/1
mg/1
mg/1
mg/1
ug/i
mg/1
ORC James Warren
Phone [phone]
Number
of
samples
________2
________1_
________1
_____£
________1_
________1
________1_
________1_
1
_____________1_
____]_
_____________1_
_____________1_
_____________1_
_____£ ~ r
i
T
_____________i_
__________i_
T
~ ~T
__________i_ r
2
i
i
7
i
7
£
1
7
£
1
7
i
Sample
Result
'• 7
8.3
5.73
0,42
0,04
424
49,1
________18
<5
SM4500CN-E
SM510A&B
624
<3,0 ug/1
<5.0 ug/1
<2.0|ug/l
<2,0
___<5
7,31
<5.0 ug/1
<.2
<10
<2.0
<5
<1.0
32.1
<,005
<.005
Town Of Clayton
Facility Name Little Creek Water Reclamation Facility
Date of sampling: 6/7/2012
Analytical Laboratory' : Pace Analytical,Enviroment 1, Town of Clayton WWTP Lab
10
0,005 mg/1
0.005 mg/1
5
5
5
5
5
10
5
5
10
5
5
5
5
5
:et
Ug/1
<5
<5
<5
<5
Page 2
«■* ’
1,1 -dichloroethylene
1.2- dichloropropane
1.3- dichloropropylene
Ethylbenzene
Methyl bromide
Methyl chloride
Methylene chloride
1,1,2,2-tetrachloroethane
Tetrachloroethylene
Toluene
1.1.1- trichloroethane
1.1.2- trichloroethane
Trichloroethylene
Vinyl chloride
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
5
5
5
5
5
5
5
5
5
10
5
5
5
5
5
5
5
ug/1
<5 ug/1
<5|ug/l
ug/l
<20|ug/l
<50 ug/l
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
<5lug/l
ug/1
ug/1
ug/1
Ug/1
ug/1
ug/1
Ug/1
<5|ug/l
ug/1
<5|ug/I
<5 ug/1
<5 ug/1
<5 ug/1
<5 ug/1
ug/1
Acenaphthene
Acenaphthylene
Anthracene
Benzidine
Benzo(a)anthracene
Benzo(a)pyrene
3,4 benzofluoranthene
Benzo(ghi)perylene
13enzo(k)fluoranthene
Bis (2-chloroethoxy) methane
Bis (2-chloroethyl) ether
Bis (2-chloroisopropyl) ether
Bis (2-ethylhexyl) phthalate
4-bromophenyl phenyl ether
Butyl benzyl phthalate
2-chloronaphthalene
4-chlorophenyl phenyl ether
5
5
5
5
20
50
5
5
5
5
10
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
<5
<5
<5
<5
<5
<5
<5 ug/1
<10
<5
<5
<5
<5
<5
<5
<5
<5
<5
<5
<5
<10
<5
<5|ug/i
<51 ug/1
<10
<5|ug/l
<5|ug/l
<5
1 Quantitation
Level _____________ _
i
7
7
_i_
i
i
7
2_
j_
_ 7
; ug/1' ; u . ./U
j.
1
7
7
7
7
_i_
i
i
7
1
1
1
2
_L
i
i
2_
i
7
7
i_
j_
i
P-chloro-m-creso
2-chlorophenol
2.4- dichlorophenol
2.4- dimethylphenol
4.6- dinitro-o-cresol
2.4- dinitrophenol
2-nitrophenol
4-nitrophenol
Pentachlorophenol
Phenol
2.4.6- trichlorophenol
Base-neut^ compounds - ' 625 conf
Grab |
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
__________ _______________________
VglatlWgrg^nic compound, (Cont.) ; •: / --
Grab | ...
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab ——— —
Grab
Grab
Grab
Grab
Grab
_5___
_5___
_5___
_5___
10__
_5___
_5___
5___
_5___
5___
5___
5___
5___
5
____amples
■i i"ru"
<5
<5
<5
<5
/ Signatu.
Date
Page 3
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
<5
<5
<5
1
1
1
1
T
i
i
i
T
i
T
i
T
i
j_
i
T
i
T
j_
i
T
7
i
7
i
ug/l
ug/1
ug/l
ug/1
ug/1
<5|ug/l
<5 ug/l
<25 ug/l
ug/l
<5|ug/l
<5 ug/l
ug/l
ug/l
ug/l
<5lug/l
<5 ug/l
<5 ug/l
<5 ug/l
<10 ug/l
<5 ug/l
<io|ug/l
ug/l
<5 ug/l
<5 ug/l
<5 ug/l
<5|ug/l
ug/l
<5 ug/l
ug/l
5
5
5
5
5
5
5
25
5
5
5
5
5
5
5
5
5
5_______
10
5_______
10
5_______
5_______
5_______
5_______
5_______
5_______
5_______
5
samples
Analytical Quantitation
_ _______ Sample Type | Method |
ounds 625confd
Sample
— I Result ment --------
/I______________
<5
<5
<5
<5
<5
«■«J ..I,;'
;tw
Chrysene
Di-n-butyl phthalate
Di-n-octyl phthalate
Dibenzo(a,h)anthracene
1.2- dichlorobenzene
1.3- dichlorobenzcne
1.4- dichlorobenzcne
3.3- dichlorobenzidine
Diethyl phthalate
Dimethyl phthalate
2.4- dinitrotoluene
2,6-dinitrotoluene
1,2-diphenylhydrazine
Fluoranthene
Fluorene
Hexachlorobenzene
Hexachlorobutadiene
Hexachlorocyclo-pentadiene
Hcxachloroethane
Indcno(l,2,3-cd)pyrene
Isophorone
Naphthalene
Nitrobenzene
N-nitrosodi-n-propylamine
N-nitrosodimcthylamine
N-nitrosodipheny lam i ne
Phenanthrene
Pyrene
1,2,4,- trichloro benzene
I certify under penalty of law that this document and all attachments were prepared under my direction
and supervision in accordance with a system to design to assure that qualified perdonnel properly
gather and evaluat the information submitted. Based on my inquiry' of the person or persons that
manage the system, or those persons directly responsibcl for gathering the information, the
information submitted is , to the best of my knowledge and belief, true, accurate and complete. I am
aware that there are significant penalties for submitting false information, including the
possibility of fines and imprisonment for knowing violations.
buA
Authorized Representative name
June 21, 2012
| THE WOOTEN COMPANY j
‘ ............. .a
on June 08, 2012. Theresults relate only to the samples included in this report.
If you have any questions concerning this report, please feel free to contact me.
Enclosures
Page 1 of 7
Kevin Herring for
MaryAnne Sims
[e-mail]
Project Manager
RE: Project: 1205-00649
Pace Project No.: 92120903
Ms. Leigh Honeycutt
Town of Clayton WWTP
Po Box 879
Clayton, NC 27528
Pace Analytical Services, Inc.
6701 Conference Drive
Raleigh. NC 27607
[phone]
Sincerely,
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc..
Dear Ms. Honeycutt:
Enclosed are the analytical results for sample(s) received by the laboratory
Analyses were performed at the Pace Analytical Services location indicated on the sample analyte
page for analysis unless otherwise footnoted.
SXilMSg
AUG 2 8 2012
^ceAnalytical’
www.pacalabs.com
ace XXna/y^t/ca/
f
Page 1 of 2
Laboratory Report
Work Order #:1205-00649
CL1535
Condition
4 +/- 2 deg C
Test Performed Method Results Qualifier
Condition
4 +/- 2 deg C
Test Performed Method Results Qualifier
No.
002
No.
001
Sample ID
Effluent Grab
Sample ID
Effluent Composite
Telephone: [phone]
Fax: [phone]
Project No.:
Project ID:
- Prepared for --
Leigh Honeycutt
Clayton WWTP, Town of
PO Box 879
Clayton, NC 27528
Date Sampled
6/7/2012
6701 Conference Drive
Raleigh, NC 27607
EPA1664A
SM 4500-CN-E
EPA 420.4
EPA 624
EPA 625
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 245.1
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
2540C
EPA 200.8
EPA 200.8
CALC.
Time Sampled
10:30
Time Sampled
10:30
<5.0 mg/L
<0.005 mg/L
<0.005 mg/L
SEE ATTACHED
SEE ATTACHED
15.9 mg/L
2.28 mg/L
49,1 mg/L
Report Date:
Date Received:
Sample Type
Grab
NC/WW Cert. #: 067
NC/DW Cert. #: 37731
Sample Type
Composite
6/27/2012
6/8/2012
6/21/12
6/21/12
6/21/12
6/15/12
6/15/12
6/15/12
6/19/12
6/15/12
6/15/12
6/15/12
6/15/12
6/15/12
6/15/12
6/12/12
6/15/12
6/15/12
6/15/12
6/15/12
6/15/12
6/15/12
6/14/12
16:51
16:51
16:51
0:00
0:00
Oil & Grease
Cyanide
Phenols
Volatile TTOs by GC/MS
Semivolatile TTPs by 625
Antimony
Arsenic
Beryllium
Cadmium
Chromium
Copper
Lead
Mercury
Selenium
Silver
Thallium
Zinc
Molybdenum
Nickel
Total Dissolved Solids
TOTAL HARDNESS (WW)
Calcium
Magnesium
Total Hardness Calculation
Date Sampled
6/7/2012
Cust. Code:
Cust. P.O.#:
Matrix
WW
Matrix
WW
01
Priority Pollutant Analysis
<3.00 ug/L
<5.00 ug/L
<2.00 ug/L
<2.00 ug/L
<5.00 ug/L
7.31 ug/L
<5.00 ug/L
<0.2 ug/L
<2.00 ug/L
<5.00 ug/L
<1.00 ug/L
32.1 ug/L
<5.00 ug/L
<10.0 ug/L
424 mg/L
16:22
16:48
16:48
16:48
16:48
16:48
16:48
15:42
16:48
16:48
16:48
16:48
16:48
16:48
15:55
0:00
10:53
10:53
10:53
Analyzed
Date Time
Analyzed
Date Time
<s»^ce ^Xn^/y-tica/
Page 2 of 2
Laboratory Report
Work Order #:1205-00649
Condition
4 +/- 2 deg C
Test Performed Method Results Qualifier
Cyanide SM 4500-CN-E 0.023 mg/L 6/21/12 16:51
Condition
4 +/- 2 deg C
Test Performed Method Results Qualifier
Reviewed by:
for Tritest, Inc.
No.
004
Arsenic
Cadmium
Chromium
Copper
Lead
Mercury
Selenium
Silver
Zinc
Molybdenum
Nickel
Sample ID
Influent Composite
Sample ID
Influent
6701 Conference Drive
Raleigh, NC 27607
EPA 200.8
ERA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 245.1
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
Time Sampled
10:00
Time Sampled
10:00
Sample Type
Composite
Sample Type
Grab
NC/WW Cert. #: 067
NC/DW Cert. #: 37731
6/15/12
6/15/12
6/15/12
6/15/12
6/15/12
6/12/12
6/15/12
6/15/12
6/15/12
6/15/12
6/15/12
No.
003
Telephone: [phone]
Fax: [phone]
Date Sampled
6/7/2012
Date Sampled
6/7/2012
<5.00 ug/L
<2.00 ug/L
<5.00 ug/L
33.9 ug/L
<5.00 ug/L
0.328 ug/L
<2.00 ug/L
<5.00 ug/L
144 ug/L
<5.00 ug/L
<5.00 ug/L
Matrix
WW
Matrix
WW
16:48
16:48
16:48
16:48
16:48
15:42
16:48
16:48
16:48
16:48
16:48
“AMENDED REPORT; CHANGED UNITS ON METALS PER CLIENT REQUEST.
7/14/12
Analyzed
Date Time
Analyzed
Date Time
ANALYTICAL RESULTS
Sample: Effluent Grab Lab ID: 92120903001 Collected: 06/07/12 10:30
Parameters Results Units DF Qualifiers
Date: 06/21/2012 09:33 AM REPORT OF LABORATORY ANALYSIS Page 4 of 7
Project:
Pace Project No.:
1205-00649
92120903
This report shall not be reproduced, except in full.
without the written consent of Pace Analytical Services. Inc..
Oil and Grease
Acenaphthene
Acenaphthylene
Anthracene
Benzo(a)anthracene
Benzo(a)pyrene
Benzo(b)fluoranthene
Benzo(g,h,i)perylene
Benzo(k)fluoranthene
4-Bromophenylphenyl ether
Butylbenzylphthalate
4-Chloro-3-methylphenol
bis(2-Chloroethoxy)methane
bis(2-Chloroethyl) ether
bis(2-Chloroisopropyl) ether
2-Chloronaphthalene
2-Chlorophenol
4-Chlorophenylphenyl ether
Chrysene
Dibenz(a,h)anthracene
3,3'-Dichlorobenzidine
2.4- Dichlorophenol
Diethylphthalate
2.4- Dimethylphenol
Dimethylphthalate
Di-n-butylphthalate
4.6- Dinitro-2-methylphenol
2.4- Dinitrophenol
2.4- Dinitrotoluene
2.6- Dinitrotoluene
Di-n-octylphthalate
bis(2-Ethylhexyl)phthalate
Fluoranthene
Fluorene
Hexachloro-1,3-butadiene
Hexachlorobenzene
Hexachlorocyclopentadiene
Hexachloroethane
lndeno(1,2,3-cd)pyrene
Isophorone
Naphthalene
Nitrobenzene
2-Nitrophenol
4-Nitrophenol
N-Nitrosodimethylamine
N-Nitroso-di-n-propylamine
N-Nitrosodiphenylamine
Pentachlorophenol
Phenanthrene
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Pace Analytical Services, Inc.
6701 Conference Drive
Raleigh, NC 27607
[phone]
Report Limit
5^0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
10.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
25.0
5.0
5.0
10.0
5.0
5.0
20.0
50.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
10.0
5.0
5.0
10.0
5.0
5.0
5.0
50.0
5.0
5.0
10.0
25.0
5.0
ND mg/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
’’ace Analytical’
wwwpac6labs.com
ANALYTICAL RESULTS
Sample: Effluent Grab Lab ID: 92120903001 Collected: 06/07/12 10:30
Parameters Results Units Report Limit DF Qualifiers
Date: 06/21/2012 09:33 AM REPORT OF LABORATORY ANALYSIS Page 5 of 7
Project:
Pace Project No.:
1205-00649
92120903
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc..
5.0
5.0
5.0
10.0
10- 120
15-120
11- 131
10-120
10-120
10-137
5.0
5.0
5.0
10.0
5.0
5.0
10.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
10.0
5.0
70-130
70-130
70-130
70-130
0.0050
0.0050
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Pace Analytical Services, Inc.
6701 Conference Drive
Raleigh. NC 27607
[phone]
Phenol
Pyrene
1,2,4-Trichlorobenzene
2.4.6- Trichlorophenol
Nitrobenzene-d5 (S)
2-Fluorobiphenyl (S)
Terphenyl-d14 (S)
Phenol-d6 (S)
2-Fluorophenol (S)
2.4.6- Tribromophenol (S)
Benzene
Bromodichloromethane
Bromoform
Bromomethane
Carbon tetrachloride
Chlorobenzene
Chloroethane
Chloroform
Chloromethane
Dibromochloromethane
1.2- Dichlorobenzene
1.3- Dichlorobenzene
1.4- Dichlorobenzene
1.1- Dichloroethane
1.2- Dichloroethane
1.1- Dichloroethene
cis-1,2-Dichloroethene
trans-1,2-Dichloroethene
1.2- Dichloropropane
cis-1,3-Dichloropropene
trans-1,3-Dichloropropene
Ethylbenzene
Methylene Chloride
1.1.2.2- Tetrachloroethane
Tetrachloroethene
Toluene
1,1,1 -Trichloroethane
1.1.2- Trichloroethane
Trichloroethene
Tri chlorofluoromethane
Vinyl chloride
Dibromofluoromethane (S)
4-Bromofluorobenzene (S)
Toluene-d8 (S)
1.2- Dichloroethane-d4 (S)
Phenol
Cyanide
ND ug/L
ND ug/L
ND ug/L
ND ug/L
68 %
74 %
81 %
23 %
36 %
87 %
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
ND ug/L
105 %
106 %
93 %
112 %
ND mg/L
ND mg/L
*ace Analytical’
wwf.pacelabs.com
ANALYTICAL RESULTS
Sample: Influent Lab ID: 92120903002 Collected: 06/07/12 10:00
Parameters Results Units Report Limit DF Qualifiers
Cyanide 0.023 mg/L 0.0050 1
Date: 06/21/2012 09:33 AM REPORT OF LABORATORY ANALYSIS Page 6 of 7
Project:
Pace Project No.:
1205-00649
92120903
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc..
Pace Analytical Services. Inc.
6701 Conference Drive
Raleigh. NC 27607
[phone]
vce Analytical’
www.paislabs.com
CERTIFICATIONS
REPORT OF LABORATORY ANALYSIS Page 2 of 7
Project:
Pace Project No.:
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc..
1205-00649
92120903
Pace Analytical Services, Inc.
6701 Conference Drive
Raleigh. NC 27607
[phone]
Asheville Certification IDs
2225 Riverside Dr., Asheville, NC 28804
Florida/NELAP Certification #: E87648
Massachusetts Certification #: M-NC030
North Carolina Drinking Water Certification #: 37712
North Carolina Wastewater Certification #: 40
South Carolina Certification #: 99030001
Virginia Certification #: 00072
West Virginia Certification #: 356
Virgina/VELAP Certification #: 460147
Connecticut Certification #: PH-0104
Florida/NELAP Certification #: E87627
Kentucky UST Certification #: 84
Louisiana DHH Drinking Water# LA 100031
West Virginia Certification #: 357
Virginia/VELAP Certification #: 460144
Charlotte Certification IDs
9800 Kincey Ave. Ste 100, Huntersville, NC 28078
North Carolina Drinking Water Certification #: 37706
North Carolina Field Services Certification #: 5342
North Carolina Wastewater Certification #: 12
South Carolina Certification #: 99006001
South Carolina Drinking Water Cert. #: 99006003
Virginia Drinking Water Certification #: 00213
we Analytical*
www.pacelabs.com
[phone]
SAMPLE ANALYTE COUNT
1205-00649
92120903
Lab ID Sample ID Method Analysts Laboratory
92120903001 Effluent Grab EPA 1664A AM1 1 PASI-C
EPA 625 BPJ 58 PASI-C
EPA 624 AW 35 PASI-C
EPA 420.4 SDH 1 PASI-A
SM 4500-CN-E JDA 1 PASI-A
92120903002 Influent SM 4500-CN-E JDA 1 PASI-A
REPORT OF LABORATORY ANALYSIS Page 3 of 7
Project:
Pace Project No.:
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc..
Analytes
Reported
Pace Analytical Services, Inc.
6701 Conference Drive
Raleigh, NC 27607
'ace Analytical’
www.pacelabs.com
QUALIFIERS
DEFINITIONS
LABORATORIES
ANALYTE QUALIFIERS
Date: 06/21/2012 09:33 AM REPORT OF LABORATORY ANALYSIS Page 7 of 7
Project:
Pace Project No.:
MO
M1
R1
PASI-A
PASI-C
Matrix spike recovery and/or matrix spike duplicate recovery was outside laboratory control limits.
Matrix spike recovery exceeded QC limits. Batch accepted based on laboratory control sample (LCS) recovery.
RPD value was outside control limits.
Pace Analytical Services - Asheville
Pace Analytical Services - Charlotte
1205-00649
92120903
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc..
Pace Analytical Services, Inc.
6701 Conference Drive
Raleigh. NC 27607
[phone]
DF - Dilution Factor, if reported, represents the factor applied to the reported data due to changes in sample preparation, dilution of
the sample aliquot, or moisture content.
ND - Not Detected at or above adjusted reporting limit.
J - Estimated concentration above the adjusted method detection limit and below the adjusted reporting limit.
MDL-Adjusted Method Detection Limit.
PRL- Pace Reporting Limit.
RL- Reporting Limit.
S - Surrogate
1,2-Diphenylhydrazine (8270 listed analyte) decomposes to Azobenzene.
Consistent with EPA guidelines, unrounded data are displayed and have been used to calculate % recovery and RPD values.
LCS(D) - Laboratory Control Sample (Duplicate)
MS(D) - Matrix Spike (Duplicate)
DUP - Sample Duplicate
RPD - Relative Percent Difference
NC - Not Calculable.
SG - Silica Gel - Clean-Up
U - Indicates the compound was analyzed for, but not detected.
N-Nitrosodiphenylamine decomposes and cannot be separated from Diphenylamine using Method 8270. The result reported for
each analyte is a combined concentration.
Acid preservation may not be appropriate for 2-Chloroethylvinyl ether, Styrene, and Vinyl chloride.
^ace Analytical'
www.pacelabs.am
Clayton WWTP, Town of
Page 1 of 1
Bill Results To:
Attn: Ms. Leigh Honeycutt
Fax: [phone]
Sampled By:
Sample Description
■
Analyses Requested Smp #
Effluent Composite C 001
Effluent Grab G WW 002
Influent G WW Cyanide 003
UP U2
Relinqui§h<
Temp:
Composite
or Grab
Project Reference:
Purchase Order #:
For Lab Use Only:
Temperatue at receipt:
Note: Rush projects
are subject to prior
approval by Lab
Attn: Leigh Honeycutt
Phone: [phone]
Report Results To:
Clayton WWTP, Town of
PO Box 879
Clayton, NC 27528
Town Of Clayton WWTP
PO Box 879
Clayton, NC 27528
(,/r/^2-
ft:SO
/O:3
4r/7/2.CV2.
I6:QO
OO
Oil & Grease, Cyanide, Phenols, Volatile TTOs by GC/MS, Semivolatile
TTOs by 625
>7-^ P -P
Priority Pollutant Analysis
•••••••••••••••••••••••••••••••••••••••••tee
tartest Chain of Custody Tntestw.o.#: 1205-00649
6701 Conference Drive, Raleigh, NC 27607
ph: [phone] fax: [phone]
NCVWV Cert # 67, NCDW Cert # 37731
Start Date
Start Time
Stop Date
Stop Time
Date
Date
Date
Matrix
WW.DW,
SW, GW etc.
WW
i „/ Received by (signatm-e) /
Received by (signature)BL
Received by (signature)
C°
□ Standard Report Delivery
I I Rush Report Delivery (w/ surcharge)
Requested Due Date:
Time
Time
Time
Antimony, Arsenic, Beryllium, Cadmium, Chromium, Copper, Lead,
Mercury, Selenium, Silver, Thallium, Zinc, Alkali Metals Prep, Metals
Prep, Molybdenum, Nickel
by (signaturef
Relinquished by (signature) ?
S^elihquished by (signature^ /
SAMPLE PRESERVATION CHECK-IN SHEET
WO#:
Checked in by:
Date:
Time:
UPSGCFEDXUSMCD
CommentsAnalysis Requested
N/A <»
[/.NoYesPos / ni :g
N/ANoPos / n Jg
N/APos/^e^No
6^0 N/ANoPos /os'
N/ANoPos/
N/ANoPos /efc
NoYes
NoYes
N/ANoesPos
N/ANoPos/,3 N/ANoPos /a N/ANoPos / neg
N/ANoPos / neg Yes
N/ANoYesPos / neg
N/ANoYesPos / neg
N/ANoYesPos / neg
N/ANoYesPos / neg
N/ANoYesPos / neg
N/ANoYesPos / neg
N/ANoYesPos / neg
N/ANoYesPos / neg
N/ANoYesPos / neg
N/ANoYesPos / neg
YesPos / neg ■) No
j__
Chlorine
Pos /^ie^
Sample
No.
Samples Preserved
Properly?
No
eid/toT
fjLbJ
o
T^oTEST
Pos./^eg^
Pos/neg\
Temp (°C):
Route:
zN7a;
rfegX
Yes
j^O'L j \ —
TOb______________
N/A
_____
TTS l/Tff’Uj)
Tritest W.O. #:1205-00649
Clayton WWTP, Town of
Page 1 of 1
Attn: Ms. Leigh Honeycutt
Fax: [phone]
Sampled By:
lai
StopTkF.ii
Effluent Composite C WW 001
Effluent Grab G
002
Influent G WW Cyanide 003
(S UP UP
Relinquished by (signatun
7Relinquished by (signature)
Relinquished by (signature Received by (signature)Date Time
Temp:
L
W'W
Project Reference:
Purchase Order #:
For Lab Use Only:
Temperatue at receipt:
Report Results To:
Clayton WWTP, Town of
PO Box 879
Clayton, NC 27528
Bill Results To:
Town Of Clayton WWTP
PO Box 879
Clayton, NC 27528
p +
Priority Pollutant Analysis
Note: Rush project
are subject to prior
approval by Lab
Date
Date
Time
7 5 *
Time
••••••••••••••••••••••••••••••••••••••••••••
test Chain of Custody
6701 Conference Drive, Raleigh, NC 27607 I
ph: [phone] fax: [phone]
NCWWCert#67, NCDW Cert # 37731
Received by (signature)
(f / 7
6r/7/ZC¥2_
1^17120^
ro-.oo
C°
I I Standard Report Delivery
□ Rush Report Delivery (w/ surcharge)
Requested Due Date:
Attn: Leigh Honeycutt
Phone: [phone]
Oil & Grease. Cyanide, Phenols, Volatile TTOs by GC/MS, Semivolatile
TTOs by 625
Antimony, Arsenic, Beryllium, Cadmium, Chromium, Copper?Lead,
Mercury, Selenium, Silver, Thallium, Zinc, Alkali Metals Prep, Metals
Prep, Molybdenum, Nickel;
10
Page 1
Number
of
samples
1
Analytical
Method
EPA350.1
Town Of Clayton
facility Name Little Creek Water Reclamation Facility
Date of sampling : 9/8/2011
Analytical Laboratory : Envrionment 1 ,Tri-Tcst
ug/»
<5 ug/I
<5 ug/l
<5 ug/l
ug/l
ug/l
<5
^5
ug/l
ug/l
mg/1
ug/l
ug/l
ug/l
ug/l
Ug/l
ug/l
ug/l
ug/l
_______1_
■______________1_
'________2
'_________1_
'_________1_
’______________1_
1
i
i
i
i
’_____________i_
’_____________i_
i
i
i r r
i
i
i
i
___i_
___i_
i r r r
—F
F
~~T
i
i
i
i
i
Paramctcr____
Ammonia (as N)
Dissolved oxygen______
Nitrate/Nitrite______________
Total Kjcldahl nitrogen______
Total Phosphorus________
Total dissolved solids 9/9/11
Hardness______________
Chlorine (total residual, TRC)
Oil and grease
Metals (total recoverable), cyanide and total phenols
Antimony
Arsenic
Beryllium
Cadmium
Chromium
Copper
Lead
Mercury
Nickel
Selenium
Silver
Thallium
Zinc
Cyanide
Total phenolic compounds
Volatile organic compounds
Acrolein
Acrylonitrile
Benzene
Bromoform
Carbon tetrachloride
Chlorobenzene
Chlorodibromomethane
Chloroethane
2-chloroethylvinyl ether
Chloroform
D ich loro b romo me thane
1.1- dichloroethane
1.2- dichloroethane
Trans-1,2-dichloroethylenc
2
5
2
10
0.2
100
10
5
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
Ug/l
Sample Type
Composite
_____Grab_____
Composite
Composite
Composite
Composite
Composite
5/20/11 Grab
Grab
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Grab
Grab
9/8/2011 ~
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
ORC James Warren
Phone [phone]
Sample
Result
<■04
_______7.6
0.91
0.8
0.9
878
_______60
_____17
<5
<50
Tso
<5
<5
<10
<5
<10
<5|ug/l
<5
I.QC 10-204 00- I
EPA420.4
EPA624 O
Units of
Measure
ment
mg/1
mg/I
mg/1
mg/1
mg/1
mg/1
mg/1
ug/l
mg/1
<3
<10
___<2
<2
<5
7.12
<10
<.2
<10
<10
<5 ug/l
<1
60.3
<.005
7
10____
.005 mg/1
.005 mg/1
ug/l
50
50
5
5
10
5
10
5
5
5
5
5
5
5
Quantitation
Level
EPA353.2__________
EPA351.2
EPA365.4
SM2540C
SM2340C__________
HACK 10014
EPA1664A
■ug/i?^
EPA200.8
EPA200.8
EPA200.8
EPA200.8
EPA200.8
EPA200.8
EPA200.8
EPA245.1
EPA200.8
EPA200.8
EPA200.8
EPA200.8
EPA200.8
Sample Type
ug/1
ug/1
ug/1
Page 2
ug/1
ug/1
Analytical
Method
Number
of
samples
Sample
Result
Units of
Measure
meat
ug/1
ug/1
ug/1
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
<5
<5
ug/1
ug/1
ug/1
ug/1
<5lug/l
ug/1
ug/1
<5|ug/l
ug/1
<5
HI
<5
<5
ug/i
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
Ug/I
ug/1
ug/1
<10|ug/l
<10
<10
<10|ug/l
<10
<10
<10
<10[ug/l
<10
<10|ug/1
<10
<10
<10 ug/1
<10 ug/1
<10
<roiug/i
<10 ug/1
<10
<10
< 10 [ ug/1
<10
<50
<50
<10|ug/l
<10
<30|ug/l
<10 ug/1
<10 ug/1
<5
<5lug/l
< 10|ug/l
<5
<5
<5
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
1
T
i
T
T
i
1
T
i_
i
T
i
EPA 625 ug/1
_1_
1
1
1,
1
T
i
i
ug7i___________________
i
T
T
i
T
i
T
T
i_
2_
j_
i
T
2,
i
T
i
________Grab
________Grab
________Grab
__ Grab
~ Grab
Grab
Grab
Grab
Grab
Grab
Grab
625
Grab
Grab
Grab
Grab
Grab
Grab
Grab
________Grab
Grab
Grab
Grab
Grab
Grab
Grab
~ Grab
Grab
Grab
_______
Volatile organic compounds (Cont.) 624
1.1 -dichlorocthylcne
1.2- dichloropropane
1.3- dichloropropylene
Ethylbenzene
Methyl bromide
Methyl chloride
Methylene chloride
1.1.2.2- tetrachlorocthane
Tetrachloroethylene
Toluene
1.1.1 -trichloroethane
1.1.2- trichloroethanc
Trichloroethylene
Vinyl chloride
Actd-extractable compounds 9/8/2011
P-chloro-m-creso
2-chlorophenol
2.4- dichlorophenol______
2.4- dimethylphenol
4.6- dinitro -o-cresol
2.4- dinitrophenol
2-nitrophenol
4-nitrophenol
Pentachlorophenol
Phenol
2.4.6- trichlorophenol
Bsscneousl compound.
Acenaphthene
Acenaphthylene
Anthracene
Benzidine
Benzo(a)anthracene
Benzo(a)pyrene
3,4 benzofluoranthene
Benzo(ghi)perylene
Benzo(k)fluoranthene
Bis (2-chloroethoxy) methane
Bis (2-chloroethyl) ether
Bis (2-chloroisopropyl) ether
Bis (2-ethylhexyl) phthalate
4-bromophenyl phenyl ether
Butyl benzyl phthalate
2-chloronaphthalene
4-chlorophcnyl phenyl ether
Quantitation
___Level
ug/1
5
5
5
5
5
5
5
5
5
5
10
5
5
5
10
10
10
10
50
50
10
10
30
10
10
Parameter <10
ug/1
ug/1
ug/1
Date
Page 3
Analytical^
Method
Number
of
samples
Units of
Measure
ment
<10
<10 ug/1
<10
<10
<10
<10 ug/1
<10 ug/1
<10|ug/l
<10
<10|ug/l
<10 ug/1
<10
<20
ug/1
ug/1
<10 ug/1
ug/1
ug/1
ug/1
ug/1
10 ug/1
ug/1
ug/1
ug/1
ug/1
Ug/1
2
2
1
T
i
2
2
2
i
T
T
2
T
T
2
2
i
i
2
2
i
i
i
2
2
i
2
i
T
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Quantitation
Level
ug/1
10
10
10
10
10
10
10
20
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
20
10
20
10
10
10
<10 Ug/1
<10 ug/1
<10
<~0
<10
< 10|ug/l
<10
< 10|ug/l
<20
TTo
<20|ug/l
<10
<To^|ug/i
<10 ug/1
/'2-I /it
I certify under penalty of law that this document and all attachments were prepared under my direction
and supervision in accordance with a system to design to assure that qualified pcrdonncl properly
gather and evaluat the information submitted. Based on my inquiry of the person or persons that
manage the system, or those persons directly responsibel for gathering the information, the
information submitted is , to the best of my knowledge and belief, true, accurate and complete. I am
aware that there are significant penalties for submitting false information, including the
possibility of fines and imprisonment for knowing violations.
Authorized Representative name
Sighature
Sample Type
Base-neutral compounds (cont.)
Chrysene
Di-n-butyl phthalate
Di-n-octyl phthalate
Dibenzo(a,h)anthracene
1.2- dichlorobenzene
1.3- dichlorobcnzcne
1.4- dichlorobenzcne
3.3- dichlorobenzidine
Diethyl phthalate
Dimethyl phthalate
2.4- dinitrotoluene
2,6-dinitrotolucne
1,2-diphenylhydrazine
Fluoranthene
Fluorene
llexachloro benzen e
Hexachlorobutadicne
Hexachlorocyclo-pentadiene
Hexachloroethanc
lndeno( 1,2,3-cd)pyrcne
Isophorone
Naphthalene
Nitrobenzene
N - nitro so di - n - pr o py lam me
N-nitrosodimethylamine
N-nitrosodiphenylamine
Phenanthrene
Pyrene
1,2,4,-trichlorobenzene
Page 1 of 4
Laboratory Report
Work Order #:1109-00090
CL1535
Matrix Condition
WW 4 +/- 2 deg C
Test Performed Method Results Qualifier
Condition
4 +/- 2 deg C
Test Performed Method Results Qualifier
EPA 624
No.
002
No.
001
Oil & Grease
Cyanide
Phenols
Volatile TTOs by GC/MS
Acrolein
Acrylonitrile
Benzene
Bromodichloromethane
Bromoform
Bromomethane
Carbon tetrachloride
Chlorobenzene
Chloroethane
2-Chloroethylvinyl ether
Chloroform
Chromium
Copper
Lead
Mercury
Selenium
Silver
Thallium
Zinc
Molybdenum
Nickel
Antimony
Arsenic
Beryllium
Cadmium
Sample ID
Effluent Grab
Sample ID
Effluent Composite
Telephone: [phone]
Fax: [phone]
Project No.:
Project ID:
6701 Conference Drive
Raleigh, NC 27607
EPA 1664A
LQC10-204-00-1-X
EPA 420.4
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 245.1
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
EPA 200.8
Time Sampled
9:00
<50 ug/L
<50 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<10 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<10 ug/L
<5 ug/L
<5.0 mg/L
<0.005 mg/L
7.0 ug/L
Cust. Code:
Cust. P.O.#:
Report Date:
Date Received:
NC/WW Cert. #: 067
NC/DW Cert. #: 37731
Sample Type
Grab
Sample Type
Composite
9/15/11
9/15/11
9/15/11
9/15/11
9/15/11
9/15/11
9/15/11
9/15/11
9/15/11
9/15/11
9/15/11
9/15/11
9/15/11
9/15/11
9/13/11
9/13/11
9/16/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
10/12/2011
9/9/2011
- Prepared for —
Leigh Honeycutt
Clayton WWTP, Town of
PO Box 879
Clayton, NC 27528
01
Priority Pollutant Analysis
Date Sampled
9/8/2011
Date Sampled
9/8/2011
Time Sampled
9:00
Matrix
WW
<3.00 ug/L
<10.0 ug/L
<2.00 ug/L
<2.00 ug/L
<5.00 ug/L
7.12 ug/L
<10.0 ug/L
<0.2 ug/L
<10.0 ug/L
<5.00 ug/L
<1.00 ug/L
60.3 ug/L
<100 ug/L
<10.0 ug/L
12:00
15:15
9:43
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
12:37
12:37
12:37
12:37
12:37
12:37
12:37
15:32
12:37
12:37
12:37
12:37
12:37
12:37
Oo
TWTEST ** AMENDED REPORT **
Analyzed
Date Time
Analyzed
Date Time
Page 2 of 4
Laboratory Report
Work Order#:1109-00090
Condition
4 +/- 2 deg C
Test Performed Method Results Qualifier
6701 Conference Drive
Raleigh, NC 27607
No.
002
Chloromethane
Dibromochioromethane
1.1- Dichloroethane
1.2- Dichloroethane
1.1- Dichloroethene
trans-1,2-Dichloroethene
1.2- Dichloropropane
cis-1,3-Dichloropropene
trans-1,3-Dichloropropene
Ethylbenzene
Methylene chloride
1.1.2.2- Tetrachloroethane
Tetrachloroethene
Toluene
1.1.1- Trichloroethane
1.1.2- Trichloroethane
Trichloroethene
Vinyl chloride
Semivolatile TTOs by 625
Acenaphthylene
Acenaphthene
Anthracene
Benzidine
Benzo(a)anthracene
Benzo(b)fluoranthene
Benzo(k)fluoranthene
Benzo(a)pyrene
Benzo(g,h,i)perylene
Benzyl butyl phthalate
Bis(2-chloroethyl)ether
Bis(2-chloroethoxy)methane
Bis(2-chloroisopropyl)ether
Bis(2-ethylhexyl)phthalate
4-Bromophenyl phenyl ether
2-Chloronaphthalene
4-Chloro-3-methylphenol
2-Chlorophenol
4-Chlorophenyl phenyl ether
Chrysene
Dibenzo(a,h)anthracene
Di-n-butyl phthalate
3,3'-Dichlorobenzidine
1.2- Dichlorobenzene
1.3- Dichlorobenzene
Sample ID
Effluent Grab
EPA 624
ERA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
Time Sampled
9:00
<10 ug/L
<10 ug/L
<10 ug/L
<50 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<20 ug/L
<10 ug/L
<10 ug/L
Sample Type
Grab
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/9/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
NC/WW Cert. #: 067
NC/DW Cert. #: 37731
Telephone: [phone]
Fax: [phone]
Date Sampled
9/8/2011
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
<10 ug/L
<5 ug/L
<5 ug/L
<5 ug/L
Matrix
ww
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
11:38
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
O
TOSTEST ** AMENDED REPORT **
Analyzed
Date Time
Page 3 of 4
Laboratory Report
Work Order #:1109-00090
Condition
4 +/- 2 deg C
Test Performed Method Results Qualifier
Condition
4 +/- 2 deg C
Test Performed Method Results Qualifier
Cyanide LQC10-204-00-1-X <0.005 mg/L 9/13/11 15:15
NICKEL ADDED PER CLIENT REQUEST.
No.
003
No.
002
1.4- Dichlorobenzene
2.4- Dichlorophenol
Diethyl phthalate
2.4- Dimethylphenol
Dimethyl phthalate
2.4- Dinitrophenol
2.4- Dinitrotoluene
2.6- Dinitrotoluene
Di-n-octyl phthalate
1,2-Diphenylhydrazine
Fluoranthene
Fluorene
Hexachlorobenzene
Hexachlorobutadiene
Hexachlorocyclopentadiene
Hexachloroethane
lndeno(1,2,3-c,d)pyrene
Isophorone
2-Methyl-4,6-dinitrophenol
Naphthalene
Nitrobenzene
2-Nitrophenol
4-Nitrophenol
N-Nitrosodimethylamine
N-Nitrosodiphenylamine
N-Nitrosodi-n-propylamine
Pentachlorophenol
Phenanthrene
Phenol
Pyrene
1.2.4- Trichlorobenzene
2.4.6- T richlorophenol
Telephone: [phone]
Fax: [phone]
“AMENDED REPORT: CHANGED DETECTION LIMITS AND UNITS ON METALS PER
CLIENT REQUEST.
Sample ID
Influent
Sample ID
Effluent Grab
6701 Conference Drive
Raleigh, NC 27607
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
Time Sampled
9:15
Time Sampled
9:00
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<50 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<50 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<20 ug/L
<20 ug/L
<30 ug/L
<10 ug/L
<10 ug/L
<10 ug/L
<20 ug/L
<10 ug/L
Sample Type
Grab
Sample Type
Grab
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
9/7/11
NC/WW Cert. #: 067
NC/DWCert. #: 37731
Date Sampled
9/8/2011
Date Sampled
9/8/2011
Matrix
WW
Matrix
WW
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
15:00
o
o
WIEST ** AMENDED REPORT **
Analyzed
Date Time
Analyzed
Date Time
Page 4 of 4
Laboratory Report
Work Order#:1109-00090
Reviewed by:
for Tritest, Inc.
Telephone: [phone]
Fax: [phone]
6701 Conference Drive
Raleigh, NC 27607
NC/WW Cert. #: 067
NC/DW Cert. #: 37731
O
0
TCoTEST ** AMENDED REPORT **
SAMPLE PRESERVATION CHECK-fN SHEET
UPSGCUSMFEDXRoute:
CommentsAnalysis Requested
N/AisI
N/ANoPos Y neg :S
N/ANoPos / neg Yes hi I L-
N/ANoPos /tneg
N/ANoPos / neg
N/ANoPos /fes;g
N/ANoPos / nig es
N/ANoYesPos / neg
N/ANoPos / neg Yes
N/ANoPos / neg Yes
N/ANoPos / neg Yes
N/ANoYesPos / neg
N/ANoPos / neg Yes
N/ANoPos / neg Yes
N/ANoYesPos / neg
N/ANoPos / neg Yes
N/ANoPos / neg Yes
N/ANoPos / neg Yes
N/ANoPos / neg Yes
N/ANoYesPos / neg
N/ANoYesPos / neg
N/ANoPos / neg Yes
N/ANoPos / neg Yes
N/ANoYesPos / neg
N/ANoPos / neg Yes
GENERAL COMMENTS:
Sample
No.
Samples Preserved
Properly?
No
ttpOj
o
TOIT8ST
Chlorine
Pos/neg^
WO#:
Checked in by:
Date:
Temp (°C):
Time:
6
j-g-
CD
Yles
' 'es
CP
Chain of Custody Tritest W.O. #:1109-00090
Clayton WWTP, Town of
Page 1 of 1
Bill Results To:
Priority Pollutant Analysis
Attn. Ms. Leigh Honeycutt
Fax: [phone]
Sampled By:
Sample Description
Analyses Requested Smp #
Composite C 001
Grab G WW 002
/ I uve-rvV'
(
^(0Temp:
4
Composite
or Grab
O Standard Report Delivery
I | Rush Report Delivery (w/ surcharge)
Requested Due Date:
Project Reference:
Purchase Order#:
For Lab Use Only:
Temperatue at receipt:
Note: Rush projects
are subject to prior
approval by Lab
Report Results To:
Clayton WWTP, Town of
PO Box 879
Clayton, NC 27528
Town Of Clayton WWTP
PO Box 879
Clayton, NC 27528
••••••••••••••••••••••••••••••••••••••••••to
o
TIBS TEST
6701 Conference Drive, Raleigh, NC 27607
ph: [phone] fax: [phone]
NCWWCert#67, NCDW Cert # 37731
Relinquished by (signature)^—TTTK
ReHnqufehed by (signature)- S
Relinquished by (signatura* /
Start Date
Start Time
0^‘5
Matrix
WW.DW,
SW, GW etc.
Ww
C°
Received by (signature)
Received by (signature)
Attn: Leigh Honeycutt
Phone: [phone]
Stop Date
Stop Time
Date
Ammonia, bHurta Nitrite, Tntntrji.ililihl NitrnjnrrTul.il Fhij rihnrijj Total*
Steoolved Golids.-^OTAL IIARDNE3S (WW), Calcium, Magnesium,
Hardneso-^alculation, Antimony, Arsenic, Beryllium, Cadmium,
Chromium, Copper, Lead, Mercury, Selenium, Silver, /*<o Jy
Thallium, Zinc, Alkali Metals Prep, Metals Prep
Oil & Grease, Cyeni'de, Phenols, Volatile TTOs by GC/MS, Semivolatile
TTOs by 625, Tob-RoGid. Chtorino; Water-
Time
Time
•••••••••••>••••••••••••••••••••••••••••••••
Chain of Custody Tritest W.O. #:1109-00090
Clayton WWTP, Town of
Page 1 of 1
Bill Results To:
Priority Pollutant Analysis
Attn: Ms. Leigh Honeycutt
Fax: [phone]
Sampled By:
Analyses Requested Smp #
C 001
Grab G WW 002
Received by (signature)Date Time
Temp: C°
Attn: I
Phone:
Composite
or Grab
Project Reference:
Purchase Order #:
For Lab Use Only:
Temperatue at receipt
Note: Rush projects
are subject to prior
approval by Lab
Report Results To:
Clayton WWTP, Town of
PO Box 879
Clayton, NC 27528
Leigh Honeycutt
: [phone]
Town Of Clayton WWTP
PO Box 879
Clayton, NC 27528
I | Standard Report Delivery
I | Rush Report Delivery (w/ surcharge)
Requested Due Date:
o
TtPlfiTEST
6701 Conference Drive, Raleigh, NC 27607
ph: [phone] fax: [phone]
NCWWCert#67, NCDW Cert # 37731
'^ece^ed^by^sign^tur^-
Received by (signature)
Start Date
Start Time
ft Ma
O^Ob
0^‘5
Matrix
WW.DW,
SW, GW etc.
WW
Relinquished by (signature)^
Relinquished by (signature)Time
Ammonia, Nrtwta Nitrite, t~tnfi5juliJ«hl Nitrj7iT-Tut.il Fln.i i|ihnruc Tutat-
Breselved CoiiGte.-^OTAL HAREJFJE3S (WW), Calcium, Magnesium, Jetal
Hardneeo-Calculation, Antimony, Arsenic, Beryllium, Cadmium,
Chromium, Copper, Lead, Mercury, Selenium, Silver, /*<q Jy
Thallium, Zinc, Alkali Metals Prep, Metals Prep z
Oil & Grease, CyanTete, Phenols, Volatile TTOs by GC/MS, Semivolatile
TTOs by 625, Tot. Roeid. Chlorino; Water
Stop Date
Stop Time
Z2W
Date
Date
Sample Description
Composite
Relinquished by (signature
I
vteveli Re
EPA350.1
<5
<5
Page 1
2J_
2
_i_
i
i
i
2
i
I
i
T
2
2
2
2
T
i
T
i
T
EPA200.8
SM31 13B
EPA200.7
SM3113B
EPA200.7
EPA200.7
SM3113B
EPA245.1
EPA200.7
SM3U3B
EPA200.7
EPA200.8
SM31 13B
EPA353.2
EPA351.2
EPA365.4
SM2540C
SM2340C
HACH10014
EPA1664A
ug/1
ug/1
ug/1
<5|ug/l
ug/1
ug/1
ug/1
< IO|ug/l
ug/1
ug/1
<5|ug/l
ug/1
<5 ug/1
ug/1
<5
<5
ug/1
ug/1
ug/1
ug/1
ug/1
7|ug/l
<5 ug/1
Ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
mg/1
mg/1
mg/1
x-ng/1
mg/1
mg/1
mg/1
mg/1
ug/1
mg/1
Acrolein
Acrylonitrile
Benzene
Bromoform
Carbon tetrachloride
Chlorobenzene
Chlorodibromomethane
Chloroethane
2-chloroethylvinyl ether
Chloroform
Dichlorobromomcthanc
1.1- dichloroethane
1.2- dichloroethane
Trans-1,2-dichloroethylene
0.07|mg/l
__9
7,01
2.96
1.9
742
73
20
5
<5
<5
<5
<3
<5
<1
<1
<5
2
2
T
2
i
i
2
2
2
i
I
i
2
2
i
-j.SampIeSTypc
Composite
______Grab
Composite
Composite
Composite
Composite
Composite
(12/23) Grab
Grab
ORC James Warren
Phone [phone]
<.2
<10
<10
<5
___<1
144
<.005
<2
<100
<50
^5
SM4500CN-E
' SM510A&B ~
: EPA624 - . - ug/.I
Antimony
Arsenic
Beryllium
Cadmium
Chromium
Copper
[Lead
Mercury
Nickel
Selenium
Silver
Thallium
Zinc
Cyanide
Total phenolic compounds
Volatile ofganie/edmpour
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Town Of Clayton
Facility Name Little Creek Water Reclamation Facility
Date of sampling 12/9/2010
Analytical Laboratory
Parameter . •
Ammonia (as N)
Dissolved oxygen
Nitrate/Nitrite____________
Total Kjeldahl nitrogen
Total Phosphorus
Total dissolved solids
Hardness
Chlorine (total residual, TRC)
Oil and grease
samples"
1
^ampIeMType
ug/l
<5
ug/l
ug/l
ug/l
ug/l
Page 2
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
ug/l
ug/l
ug/l
ug/l
ug/l
Ug/l
Ug/l
ug/l
ug/l
Ug/l
ug/l
ug/l
1
T
1
1
1
T
1
1
2_
1
7
T
1
£
2_
1
P-chloro-m-creso
2-chlorophenol
2.4- dichlorophenol
2.4- diinethylphenol
4.6- dinitro-o-cresol
2.4- dinitrophenol
2-nitrophenol
4-nitrophenol
Pentachlorophenol
Phenol
2.4.6- trichlorophenol
ug/l
<10 ug/l
ug/l
ug/l
ug/l
<5 ug/l
<5 ug/l
ug/l
<5lug/l
<10 ug/l
<5
<5|ug/l
<5 ug/l
<5|ug/l
<10
<10
<5
<5
<20
<10
<10|ug/l
<10 ug/l
<50 ug/l
<50
<10
<50
<50
<10 ug/l
<10
<10
<10
<10
<100|ug/l
<10
<10|ug/l
<10
<10|ug/l
<10 ug/l
<10 ug/l
<10 ug/l
<10 ug/l
<20
<10
<10
<10 ug/l
<10
Parameter'^A'jr’ Sr — “,l-- ;• - A-x
VdlatUe^rgani^bni^buhds (Cont.) 624
1.1- dichloroethylene
1.2- dichloropropane
1.3- dichloropropylene
Ethylbenzene
Methyl bromide
Methyl chloride
Methylene chloride
1,1,2,2-tetrachloroethane
Tetrachloroethylene
Toluene
1.1.1- trichloroethane
1.1.2- trichloroethane
Trichloroethylene
Vinyl chloride
Acenaphthene
Acenaphthylene
Anthracene
Benzidine
Benzo(a)anthracene
Benzo(a)pyrene
3,4 benzofluoranthene
Benzo(ghi)perylene
Benzo(k)fluoranthcne
Bis (2-chloroethoxy) methane
Bis (2-chloroethyl) ether
Bis (2-chloroisopropyl) ether
Bis (2-cthylhexyl) phthalate
4-bromophenyl phenyl ether
Butyl benzyl phthalate
2-chloronaphthalene
4-chlorophenyl phenyl ether
'amp::esi«eyel
loundsgcpi
ug/1
Ug/l
(Signature
Date
Page 3
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
ug/1
<10|ug/l
< 10|ug/l
<10
ug/1
ug/1
ug/1
ug/1
ug/1
Ug/1
ug/1
ug/1
ug/1
1
1
1
1
1
1
1
1
1
T
_1
T
_1
_1_
T
T
1
_1
1
1
_i_
2
i
i
T
2
2
i
<10
<10
<10
<10
<10
<10
<10
<10 Ug/1
<10 Ug/1
<10
<10
<10
<10 ug/1
<10 ug/1
<10
<10
<10 ug/1
<10 ug/1
<10 ug/1
<10 ug/1
<10
<10
<10|ug/l
<10
<To~ ug/1
<10
ug/1
ug/1
ug/1
ug/1
ug/1
Base-neu
Chrysene
Di-n-butyl phthalate
Di-n-octyl phthalate
Dibenzo(a,h)anthracene
1.2- dichlorobcnzene
1.3- dichlorobenzene
1.4- dichlorobenzene
3.3- dichlorobenzidine
Diethyl phthalate
Dimethyl phthalate
2.4- dinitrotoluene
2,6-dinitrotoluene
1,2-diphenylhydrazine
Fluoranthene
Fluorene
Hexachlorobenzene
Hexachlorobutadiene
Hexachlorocyclo-pentadiene
Hexachloroethane
Indcnof 1,2,3-cd)pyrene
Isophorone
Naphthalene
Nitrobenzene
N-nitrosodi-n-propylamine
N-nitrosodimethylamine
N-nitrosodiphenylamine
Phenanthrene
Pyrene
1,2,4,-trichlorobenzene
Authorized Representative name
I certify under penalty of law that this document and all attachments were prepared under my direction
and supervision in accordance with a system to design to assure that qualified perdonnel properly
gather and evaluat the information submitted. Based on my inquiry of the person or persons that
manage the system, or those persons directly responsibel for gathering the information, the
information submitted is , to the best of my knowledge and belief, true, accurate and complete. I am
aware that there are significant penalties for submitting false information, including the
possibility of fines and imprisonment for knowing violations.
[EowowffiwG U, OnDsoTfpotr^Qocd]
ID#:890 C
REVIEWED BY:
Influent Effluent
PARAMETERS
649
<0.005
<5.0
COD, mg/1
Anunonia Nitrogen as N, mg/1
Total Kjeldahl Nitrogen as N,mg/1
Nitrate-Nitrite as N, mg/1
Total Phosphorus as P, mg/1
Oil & Grease (HEM), mg/I
Phenol, ug/1
Total Cyanide, mg/1
Total Hardness, mg/1
Total Dissolved Residue, mg/1
Antimony, ug/1
Arsenic, ug/1
Beryllium, ug/1
Cadmium, ug/1
Copper, ug/1
Total Chromium, ug/I
Lead, ug/1
Mercury, ug/1
Molybdenum, ug/1
Nickel, ug/1
Selenium, ug/1
Silver, ug/1
Thallium, ug/1
Zinc, ug/1
Total Nitrogen, mg/1
213
57.47
Method
Code
DATE COLLECTED:
DATE REPORTED :
54.80
2.67
5.56
12/22/10
12/15/10
12/16/10
12/15/10
12/16/10
12/17/10
12/16/10
12/13/10
12/17/10
12/17/10
12/20/10
12/16/10
12/22/10
12/17/10
12/20/10
12/14/10
12/13/10
12/10/10
12/13/10
12/14/10
12/10/10
12/13/10
12/15/10
TRB
ANO
ANO
ANO
MPH
SEJ
SEJ
SEJ
TRB
EPA200.8
SM3113B
EPA200.7
SM3113B
EPA200.7
EPA200.7
SM3113B
EPA245.1
EPA200.7
SM3111B
SM3113B
EPA200.7
EPA200.8
SM3111B
HACH8000
EPA350.1
EPA351.2
EPA353.2
EPA365.4
EPA1664A
SM510A&B
SM4500 CN-E
SM2340C
PHONE [phone]
FAX [phone]
12/09/10
12/29/10
<1.0
35
<5.0
<5.0
<0.2
<10
<10
<10
<5.0
26
0.07
2.96
7.01
1.90
<5.0
<2
<0.005
73
Missing
<3.0
<5.0
<1.0
<1.0
7
<5.0
<5.0
<0.2
<10
<10
<10
<5.0
<1.0
144
9.97
RO. BOX 7085, 114 OAKMONT DRIVE
GREENVILLE, N.C. 27835-7085
CMF
CMF
LFJ
CMF
LFJ
LFJ
CMF
ADD
LFJ
LFJ
CMF
LFJ
CMF
ADD
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON ,NC 27528
Analysis
Date Analyst
Drinking Water ID: 37715
Wastewater ID: 10
Erowoiroimmgroti Dtwr^wgitkgd]
■ . ■
CLIENT:CLIENT ID:890 C
27528
REVIEWED BY:
Effluent
PAR/VMETERS, ug/1
I
1
Chlorolnethane
Vinyl Chloride
Bromomethane
Chloroethane
T richlorofluoromethane
1.1- Dichloroethane
Methylene Chloride
trans-1,2-Dichloroethene
1.1- Dichloroethene
Chloroform
1,1,1 -T richloroethane
Carbon Tetrachloride
Benzene
1.2- Dichloroethane
Trichloroethene
1.2- Dichloropropane
Bromodichloromethane
2-Chloroethylvinyl Ether
cis-1,3-D>chloropropene
Toluene
trans-1,3-Dichloropropene
1.1.2- Trichloroethane
Tetrachloroethene
Dibromochloromethane
Chlorobenzene
Ethylbenzene
Bromoform
1.1.2.2- Tetrachloroethane
1.3- Dichlorobenzene
1.4- DichIorobenzene
1.2- Dichlorobenzene
Acrolein
Acrylonitrile
VOLATILE ORGANICS
ERA METHOD 624
<10.00
<10.00
<10.00
<10.00
<5.00
<5.00
<10.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<100.00
<50.00
MAO
12/09/10
12/10/10
12/29/10
PHONE [phone]
FAX [phone]
Drinking Water ID: 37715
Wastewater ID: 10
n
2*1
it
b
a. i'
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
ANALYST:
DATE COLLECTED:
DATE ANALYZED:
DATE REPORTED:
P.O. BOX 7085. 114 OAKMONT DRIVE
GREENVILLE, N.C. 27835-7085
/«•
* o
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
Efflwoffliwft X heiwprtgto]
CLIENT:CLIENT ID:890 C
27528 Page: 1
REVIEWED BY:
Effluent
PARAMETERS, ug/1
SEMIVOLATILE ORGANICS
EPA METHOD 625
ANALYST:
DATE COLLECTED:
DATE EXTRACTED:
DATE ANALYZED:
DATE REPORTED:
CHS
12/09/10
12/10/10
12/28/10
12/29/10
PHONE [phone]
FAX [phone]
Drinking Water ID: 37715
Wastewater ID: 10
'ST
Ik
tv
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<20.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<50.00
<50.00
<10.00
<10.00
<10.00
<10.00
<50.00
<10.00
<10.00
<10.00
<50.00
<10.00
<10.00
<10.00
<10.00
<100.00
<10.00
<10.00
<10.00
<10.00
<10.00
P.O. BOX 7085'; 114 OAKMONT DRIVE
GREENVILLE, N.C. 27835-7085
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
t o
33
wi
9
$
It
2.7
<3
3^
HO
u r
31
MS
3 7
i
1. N-Nitrosodimethylamine
2. Phenol
3. Bis(2-Chloroethyl) Ether
4. 2-Chlorophenol
5. 1,3-Dichlorobenzene
6. 1,4-Dichlorobenzene
7. 1,2-Dichlorobenzene
8. Bis(2-Chloroisopropyl) Ether
9. Hexachloroethane
10. N-Nitrosodi-N-Propylamine
11. Nitrobenzene
12. Isophorone
13. 2-Nitrophenol
14. 2,4-Dimethylphenol
15. Bis(2-Chloroethoxy) Methane
16. 2,4-Dichlorophenol
17. 1,2,4-Trichlorobenzene
18. Naphthalene
19. Hexachlorobutadiene
20. 4-Chloro-3-Methylphenol
21. Hexachlorocyciopentadiene
22. JI,4,6-Trichlorophenol
23. 2-Chloronaphthalene
Acenaphthylene
Dimethylphthalate
2.6- Dinitrotoulene
Acenaphthene
2.4- Dinitrophenol
4-Nitrophenol
2.4- Dinitrotoluene
Fluorene
Diethylphthalate
4-Chlorophenyl Phenyl Ether
4.6- Dinitro-2-Methylphenol
N-Nitrosodiphenylamine
4-Bromophenyl Phenyl Ether
Hexachlorobenzene
Pentachlorophenol
Phenanthrene
Anthracene _
Di-N-Butylphthalate
Fluoranthene
Benzidine
Pyrene
Butylbenzylphthlate
Benzo[a]anthracene
3,3-Dichlorobenzadine
Chrysene
CLIENT:CLIENT ID:890 C
27528 Page: 2
REVIEWED BY:
Effluent
PARAMETERS, ug/1
Bis(2-Ethylhexyl)phthalate
Di-N-Octylphthalate
Benzo[b]fluoranthene
Benzo[k]fluoranthene
Benzo[a]pyrene
Indeno(l,2,3-C,d)pyrene
Dibenzo[a,h]anthracene
Benzo[g,h,i]perylene
1,2-Diphenylhy drazine
SEMIVOLATILE ORGANICS
EPA METHOD 625
ANALYST:
DATE COLLECTED:
DATE EXTRACTED:
DATE ANALYZED:
DATE REPORTED:
CHS
12/09/10
12/10/10
12/28/10
12/29/10
PHONE [phone]
FAX [phone]
Drinking Water ID: 37715
Wastewater ID: 10
<20.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
P.O. BOX 7085. 11.4 OAKMONT DRIVE
GREENVILLE, N.C. 27835-7085
49.
50.
51.
52.
53.
54.
55.
56.
57.
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
z
5 i
Is
f—f—
'■T I"jpf ■<'
^1 z'I OF CvONTAIMERS z
Ammonia Nitro.n
TKN n "C
Nitrate-Nitrite -5
T. Phosphorus n -o
O&G (HEM)n
0 0
C "5
0 ■0
-o
4 0
625 Duplicate 1 0
625 Duplicate 2 >0
APA
<0 EPA 624 (APA)W 0
T
624 Duplicate 1 w 0
PARAMETERS
£ I
bJ1X3
6
I. t
I
T
r
i
—i..
_L
<
*
i
>
<?' z m
cn
GO
o
1>
$-
w
3
c
o
z > o
X
il
4O
Z
i o
£
5
a-o
ioI m
z
O
p»E
32
oe
©
0
3 m
co
z
~D
CO
ra m o d1
£ o
i CD
>
>
n
I
g
m
£
m
o
0
bj
<j\
to
'JI
'ji
O\
o X
£
-S
i r
l-i I
I
CD
X
„5
C^
to
DC
s
Ifrn| —
2? __t*»
o
3io
£
m
ii
a
I
2u
Ki
E
II m
x
x o m
TS
DDU
X o
i
m
co mg o z
..II
....«
■ Phenol
- ,. ., tx&aSJ—---------------x !■
\m\------
s t
3 K
o
X m
g
x m
CD mI o z
I
§ I IIII
Mi I
1
>
8*8?
ES z a> o
m
Ll
K
t)
a
z
i>
X
g c
§
m
m z
z: z)
m >
!Z Z
X -Z.
-< m o
o
S z z
z 0
1 m x
8
I
z m x
3
fn
3
E
Z m
N
£O
A
r<»
Metals
I g j EPA 625
J
_
____
■
Cyanide
Hardness
Bl
' a I
o
<X X z >
2 Z
.8 8
o cz co
o
5 s
>
2 —B goz -
M fr-
3
s?
g-3
II
® in
-' T>
S’- 57
2--
2- rt
CD =
•Oj o' rs
I » zr oo
■§ 3
i> a 5
® CD
S 0
8 i Cf>
CD
CL
---------4----------- ---------1 _
IiNXS® §1I x^Yn —•II
8
0 X £ H
■< w F- §
z M 0
- ' r>-<
D z
X
I-------j.
N
j
~lr sfe SI
C\°-j
|T
11—1
•H
z
gxg£
00
r°
g
I
I.
o
3
■K
# OF CONTAINERS
Ammonia Nitro.n
TKN n "C
Nitrate-Nitrite n
T. Phosphorus n -a
O&G (HEM)n
Phenol n o
Cyanide o 15
Hardness n -a
>-0
Metals >"0
EPA 625 >0
C
625 Duplicate 1 >0
625 Duplicate 2 >0
APA
■ EPA 624 (APA)W 0
J 624 Duplicate 1 P5 0
PARAMETERS
o_
tu
1X3
K>
X
TEMPERATURE, °C
AT COLLECTION
TOTAL CHLORINE, mgl
AT COLLECTION
o
cnO
Z o z m
O z
GF1
CO
UD
O
s m m
1>
'0
m
ZE o
>
zo2m
C <
2?
*
4
O
=j
m
□3
ZE
Z O
o
1
ZE
15
P
GQ
G>
-Z.
i o
§
"b
u 3
(» &
r-
A
m
PI
3
C o a
'-A
O'
6 o.
OJ
03
’O>
O'.
O'roO co
ZD
2
5.
§
8
OT
s
I
1
81
3s
c2
S m
13
m co
zn m om
m o
i CD
3
QC
C
©
0
n
x
O'
N3
2-
o
T
N>
Oh
NJ
m> co m
ZD
m
o
»' *
•—*■
§
g
§
CD
S'
■—*-
c75‘
O'
o
CD
©
CD
03
CD
<v>
0 o s o
zd
O z
O p rr
3
o3
O 71
§
£
8
03
a
i
o X m O
X
Ol '-n w—
U1
CJ
C\
n r
rxn H z
r?
n x
b
72
z m
o X
5
X z m
2
7!
I
O
o i
2
ico sI o
I
§
Gj
O
8
I
2tnx
m
§
□ DDE
e o
i
CD m g o 2
O X m
S
£
s
CD m
I o2
‘tib-
— §sm
T.
-=3 co
8 s
3 8
^8
C3
CO
<
S
** x
h
3
JI
o X
2
2 0
iX
8 m
8
w K -
0 "0 2
*< CO ‘ ■
H § >
fx
2 O
> o
m
0
z
II
iI m x
x o m a>
-J
m
i
K>
i
X>
-:V
«
8
I 8 ll
0 co a
¥ <1
TD5 £
5- Q w 3 g o'
O O < o
2, 3
CD
£
Q
d OA o •
2 0 i 02
O ?
NJ —
DO -t-
Ch
30
o O X cz > X z:
^8
-< ^8
=5 CD
fl z p b
o e m >
0 2
X 2 -< m
O
<f h
CD 05
CD O
-O -
S o co
oT
7 I
o
CD o
><
i
X
m
8
o2
o
5;
A
m
til
§0
S Eh
S go
z z
DISINFECTIONPhone [phone] • Fax [phone] CHLORINE NEUTRALIZED AT COLLECTION
CHLORINE
CLIENT:890 C Week: 1 pH CHECK (LAB)uv
CONTAINER TYPE, P/GNONEG
CHEMICAL PRESERVATION E
A-NONE D-NAOH
[phone] E-HCL
F- ZINC ACETATE
G- NA THIOSULFATE! SAMPLE LOCATION DATE
■16 CLASSIFICATION:
WASTEWATER (NPDES)
i
DRINKING WATER
SAMPLES RECEIVED IN LAB AT °C
RECEIVED BY (SIG.)COMMENTS:
WCEIVED BY (SIG.)
RELINQUISHED BY (SIG.)RECEIVED BY (SIG.)
PLEASE READ Instructions for completing this form on the reverse side.
N? 215389FORM «5
DATETIME
I
DATETIME
I
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON NC 27528
Sampler must place a “C" for composite sample or a “G" for
Grab sample in the blocks above for each parameter requested.
SAMPLES COLLECTED BY:
(Please Print)
CHAIN OF CUSTODY MAINTAINED
DURING SHIPMENT/DELIVERY
Y N
P
KJ
3
‘S.
3 Q
□
cn az
EI
£
DATETIME
DATETIME
Effluent
i
LU
X LU
•=* o. o££
• ••••••• ••••••••••• • • • • •
Greenville, NC 27858 Page 2 of 2_
cn azLU
I o o
o
at
COLLECTION
------- g
TIME
C-H?SO4
9
cc Q
2 O
b-hno3
RELINQUISHED BY (SIG.) (SAMPLER)
REUNQUISHEDBY<§IG?)
Q| SOLID WASTE SECTION
□ DWQ/GW
DATETIME
| ?
IZ-^O\ /:§-Q
DATETIME
Toxicity Testing Data
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 09/19/12
Facility: CLAYTON V7WTP NPDES#: NC0025453 Pipe#: 001 County: JOHNSTON
Lab<
Comments: dilution water batch 34
and 35 used.
Work Order:
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
% Mortality Avg.Reprod.
# Young Produced 17 16 16 19 1718 18 17 17 19 17 19
Adult (Dive (D)ead L L L L LLL L L L L L
1 2 3 4 5 6 87 9 10 11 12
PASS FAIL
# Young Produced 19 19 16 18 16 16 17 16 17 18 18 16 X
Check One
Adult (L)ive (D)ead L L L L L L I.L L L L L
1st sample 1st sample 2nd sample
PH
Control 8.14 8 .17 8.07 8.17 8.20 8.11
Sample 2: 09/12/12
Treatment 2 8.14 8.15 8.20 8.05
1st
Grab DurationComp.
Sample 1 X 22.8 hrs
Sample 2 X 24.5 hrs
Hardness(mg/1)44
7.84 7.68 7.87 7.61 7.79 7.58
Spec. Cond.(pmhos)181 899 1117
Treatment 2 7.81 7.59 7.82 7.59 7.74 7.58
Chlorine(mg/1)<0.1 <0.1
LC50/Acute Toxicity Test Sample temp, at receipt(°C)0.7 1.1
(Mortality expressed as %, combining replicates)
%%%%%%%%%%Concentration
%%%%%%%%%%
start/end
Control
PHD.O.
Organism Tested:Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT-1 (3/87)
Control CV
6.212%
Chronic Test Results
Calculated t = 0.715
Tabular t = 2.508
% Reduction = 1.90
High
Cone.
D
I
L
U
T
S
A
M
P
2nd
P/F
Effluent %: 2%
TREATMENT 2 ORGANISMS
0.00
Treatment 2
0.00
Control
17.17
Treatment 2
17.50
Control
S
A
M
P
e
n
d
e
n
d
LC5 0 = ______%
95% Confidence Limits
% %
e
n
d
% control orgs
producing 3rd
brood
100%
8.21
____
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
D.O.
Control
I
Note: Please
Complete This
Section Also
s
t
a
r
t
2nd sample
s
t
a
r
t
1st sample
t
1st sample
I8’15
s
t
a
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
Complete This For Either Test
Test Start Date: 09/12/12
Collection (Start) Date
Sample 1: 09/10/12 ;
Sample Type/Duration
Mortality
start/end
Method of Determination
Moving Average Probit
Spearman Karber Other
rev. 11/95 (DUBIA ver. 4.41)
* PASSED: 1.90% Reduction *
y Performing Test: MERITECH LABS, INC.
x .
/Signature of Operator in Responsible Charge
ly j a ■
Signature of Laboratory Supervisor
Sample 2:Sample 1:
Sample Information
G/C?
C
Batch #3$
itL /L.C-2
17Total Produced
ni?
!•/ZZZ]
pH1
Control
L
Sample
D.O.2nd Sample
Control
Sample
L
Temp.
L i /6 u si /6| /6i n i/'fe i/ zwinriTotal Produced
Control
Comments:
Sample
1
7
5
MH
2 3 4
i
8
O
c_
Sample 1
Sample 2
Day #2
# Young Produced
Adults Live / Dead
_____Day #5_____
# Young Produced
Adults Live / Dead
Day #7
■------------------==±S=5=XS__------------ ---
# Young Produced
Adults Live / Dead
4
o
4
o
L-
7
o
L
11
O
Transfer Day
Hardness
(mg/L)
Spec. Cond.
(umhos/cm)
Chlorine
(mg/L)
Receipt Sample
Temp. (°C)
100% pH
8.^7
T7Z
6
o
I.
9
O
c
sis
2nd Sample
213 U&
# Young Produced
Adults Live / Dead
Day #5
# Young Produced
Adults Live / Dead
Day #7
# Young Produced
Adults Live / Dead
10
O
L
10
o
C| V L
2nd Sample
5
Q
Client:
NPDES #: NC
Dilution Water: Lake Hunt
Test Organism Source: Tray #
Stirred / Aerated for D.O.: Y
Control Organism Reproduction
_______Day #2_______ 1- ___
1 ..
9
o
L
8
o
L
11
C
12
o
3
o
1st Sample
mitiHl final
Sample
2
___
it
Sample
1
/-
2nd Sample
VP wl
initial finalinitial final
??■■? 7,6/
7^ 7^
initial final
Incubator #:
Date End:
Time End:
__ Time:
Time:
Reviewed by:
••••••••••••••••••••••••••••••••••••••••••••
A
initial final
1st Sample
S',/1-/ g.'l
initial ?irai
0
ijil
777^77 ■ ■■o.?
1st Sample
7^ 7.6^
wTtZT
initial final
5
o
L~
r.1/ 5? ■ 6/ ■ 7 8/.. 9/
Ll-I u IL |l_ I 7 LI u I
1 2 3 4 5
pwi 77
L_
6 7 8
|7 |7
c u
9 10
2
7
1; 2/ 3, 4, 5; e 7, 8Z
a/- yr..-'//, Y?i 7,71 y'/iy?
7 Q — I— L_ t- (
3 4 5 6 7 A 9/ 1Q
El 71 | / Iffil 5?
U
6 7
LpI &
L-
2nd Sample
Y.TpJ®
7.77 758
initial final
Duration
22.3^ hours
hours
KUAI/Y I ^1/71^1/71/71/^1/711/1 I
Percent of Control producing third brood: £)£?%
Test Sample Organism Reproduction

| Effluent %:
' 1st Renewal Date:
1 2nd Renewal Date:
Analyst(s):
Collection (Start) Dates:
7%//2-12
O
L
1Q 11z 12
L- L
11 12
1
2nd Sample
w g.n
9.i7 ‘t.-go
initial final
Transferred by: Fed by;
DayO /Y«
Day 1
Day 2 {^- ^4-
Day 3 -
Day 4
Day5 W'l- rAV-
Day 6
Terminated by
2 3
o| O
U It-
2
Meritech, Inc.
i , j Mini Chronic Pass/Fail Test: Ceriodaphnia dubia
Wv ---------------- Pipe #; County: Date Start: ^'11 -[I
Date/Time of Culture Transfer: ) /ZP ; c> 3M Time Start:
Date / Time Neonates born: 9-/) - A?; J st Renewal Date: Q - /«/ 71
Age of Neonates at Test Starts hours / 2nd Renewal Date: ~
Randomized:^/ N Culture Tray Temp: Analyst(s): mer.MR.^T
Meritech Sample ID #
Fax: [phone]
Email: txmrtech(a)bellsouth.net Web Site: www.meritech-labs.com
SAMPLE INFORMATION
Sample Site:
Sample Type: Grab ® Composite # of containers:
Sampling Time: Start Date 9 - IO "2.g / 2 Start Time.AM PM
7 -//-End Date:End Time: AM. PM
TOXICITY TEST INFORMATION
IWC:%Test Concentrations (if multiple dilutions).
Comments:

Date.Time.PM
Date:Time: PM
Date:Time: AM M
Date: Time: AM PM
Date Time: AM PM
Date: Time: AM PM
Date: Time. AM PM
Date: Time: AM PM
Sample Temperature (°C):
Fed EX
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by
Received by.Date: Time: AM
Sample Temperatures (°C):I Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by^-'**1*5^
Relinquished by:x^*xs:^'
Received by:
Relinquished by
Test Required: Chronic (7 days)
Lj Acute (24-48 hours)
City:
County-
Test Organism: Ceriodaphnia dubia (water flea)
^4 Pimephales promelas (fathead minnow)
Mysidopsis bahia (shrimp)
II: 30
V'/ t'i.
/I-'40
PO#-__________________
NPDES# NC 002.^^3
PhOne;
Pipe#: soj
zip.
MERITECH, INC.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
- - 4 _ v
c>.'7 / IV7 /
CLIENT INFORMATION
Ad_________________________
_____________/ AS-1 ’p tZWj
8^ Y 8- ? 9 '/7)
sj O A Xi S r/<?
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
SAMPLE CONTAINERS ARE TO BE COMPLETEL Y FULL {no air space), CHILLED AND COVERED WITH ICE
Collector's Name: Print:_____
Client: d{.
Contact Person.
Address.
Method of Shipment: UPS Fed EX Meritech Pick-up
Samples shipped on rriday must be FedEx and must be clearly labeled for Saturday delivery **
K T v L S
A. A Ay p
State- 4/£-
SHIPP1NG INFORMATION
?//1/^0/ 0L
7//f//^
Vi I /7
PM
Merrtech Sample ID #
Fax. [phone]
Email: txmrtech(a)bellsouth.net Web Site: wivw.meritech-labs.com
Client P0#
Contact Person
Address:
State.
SAMPLE INFORMATION
Sample Site:
CKi Composite GrabSample Type:# of containers:
Sampling Time:Start Date. Start Time. AM PM
End Date:/ O'.OOEnd Time 4^, PM
Signature
TOXICITY TEST INFORMATION
Mysidopsis bahia (shrimp)
8WC:%Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION //^
Timef*^jRelinquished by.& 'Date: AM PM
Received by: Date: Time PM
Relinquished by: Date: Time AM
Received by: Date: Time AM PM
Relinquished by Date Time AM PM
Received by. Date. PMTime: AM
Relinquished by PMDate. Time AM
Received by: Date: Time AM PM
Sample Temperature (°C)'
SAMPLE RECEIVING (Laboratory Use Only)
\<J^‘
Relinquished by
T/js/io iHsr/'Received by,Date:Time:AM
/ i.l i I Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
Test Required: jXl Chronic (7 days)
LJ Acute (24-48 hours)
Test Organism: Ceriodaphnia dubia (water flea)
j/4 Pimephales promelas (fathead minnow)
-------- CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
£2
35
cr//3/2.o/ 2—
NPDES#: NC
Phone.
Pipe #. (
zip & _______
MERITECH, 6NC.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
0^
Sample Temperatures^^)^ I « 1
A_L d
Method of Shipment: UPS Q Fed EX O Merilech Pick-up
” Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery ""
** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED AND COVERED WITH ICE *“
Collector's Name: Print: -e. t'-OjpC.{3$
CLIENT INFORMATION
«s>
cr /jLe. tr Jy
? O . z?oX ^ 77
City: (5 /*7
County 7^4 £ 5 n
PM
M
Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 09/19/12
Facility: CLAYTON WWTP NPDES#: NC0025453 Pipe#: 001 County:JOHNSTON
Laboratory Performing Test: MERITECH LABS,INC.
Reduction:1.90%2% EffluentCONTROL
# Replicates 12 12
Female Live 12 12
Adult Male 0 0
Adult Dead 0 0
Adult Mortality 0.00%0.00%
# Neonates 210 206
Mean # Neonates 17.500 17.167
Standard Deviation 1.087 1.193
Coefficient of Variation 6.212%
Fisher's Exact Test
A - 12 B = 12 b = 12a = 12
a/A = 1.00 b/B = 1.00
Success is: survival
Critical b value = 8
12 8
Test Passes!
SHAPIRO-WILK 1S TEST FOR NORMAL DISTRIBUTION OF DATA
ORDERED OBSERVATIONS
i Group Centered iNeonates Group CenteredNeonates
C
C
E
E
E
E
E
C
C
C
C
C
-1.5000
-1.5000
-1.1667
-1.1667
-1.1667
-1.1667
-1.1667
-0.5000
-0.5000
-0.5000
-0.5000
-0.5000
13
14
15
16
17
18
19
20
21
22
23
24
E
E
C
C
E
E
E
C
C
C
E
E
17
17
18
18
18
18
18
19
19
19
19
19
-0.1667
-0.1667
0.5000
0.5000
0.8333
0.8333
0.8333
1.5000
1.5000
1.5000
1.8333
1.8333
1
2
3
4
5
6
7
8
9
10
11
12
16
16
16
16
16
16
16
17
17
17
17
17
The test concludes that the proportion of survival is not
significantly different for the control and the effluent groups.
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.)
COEFFICIENTS AND DIFFERENCES
i x(n-i-1)x(i)a(i)x(n-i-1)x(i)
1
W X 25.9504
28.6667
Calculated W = 0.905 Critical W = 0.884
0.905 z 0.884
The reproduction data is normally distributed
evaluated at a 99% confidence interval.
Test Passes!
F test for Homogeneity of Variance
Effluent variance 1.4242
F == 1.21
Control variance 1.1818
Numerator degrees of freedom:11 Denominator degrees of freedom:11
Critical F = 5.32
1.21 5.3 2 =► The Test the variances of the two groupsPASSES,s
are significantly the same, homogeneous.
1.8333
1.8333
1.5000
1.5000
1.5000
0.8333
0.8333
0.8333
0.5000
0.5000
-0.1667
-0.1667
0.4493
0.3098
0.2554
0.2145
0.1807
0.1512
0.1245
0.0997
0.0764
0.0539
0.0321
0.0107
3.3333
3.3333
2.6667
2.6667
2.6667
2.0000
2.0000
1.3333
1.0000
1.0000
0.3333
0.3333
1
2
3
4
5
6
7
8
9
10
11
12
-1.5000
-1.5000
-1.1667
-1.1667
-1.1667
-1.1667
-1.1667
-0.5000
-0.5000
-0.5000
-0.5000
-0.5000
EQUAL VARIANCE t TEST
17.5 17.2
t == 0.715
0.466
Degrees of freedom = 22
Critical t = 2.508
0.715 < 2.508
There is not a significant difference in reproduction
Chronic Test PASSES
Test passed. There is not a significant difference in reproduction
between the Control and the effluent evaluated at a 99% confidence interval.
Date 9/21/2012Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test
Facility Clayton NPDES # NC00 25453 Pipe#. 001 County. Johnston
Comments|Laborati
x
Signature of Laboratory Supervisor
MAH ORIGIN Al TO:
Test Initiation Date/Time 9/11/2012 3.35 PM Avg Wt/Surv Control 0.759 Test Organisms
% Survival 100.0
Hatch Date 9/10/12Avg Wt (mg)0.759
% Survival 97.5 Hatch Time: 3:00 pm CT
0 752Avg Wt (mg)
1 % Survival 100 0
Avg Wt (mg)0.755
I 2 % Survival 100.0
Avg Wt (mg)0.777
4 % Survival 92 5
0 692Avg Wt (mg)
8 % Survival 100.0
Avg Wt (mg)0 771
]>8
DWQ Form AT-5 (1/04)
Dilution H2O Batch #
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chiorine(mg/L)
Temp, at Receipt (°C)
| 0.5 [ Surviving #
Original #
Wt/original (mg)
H Surviving #
Original #
Wt/original (mg)
J Surviving #
Original #
Wt/original (mg)
I Surviving #
Original #
Wt/original (mg)
I Surviving #
Original #
Wt/onginal (mg)
r Cultured In-House
Outside Supplier
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Normal
Hom Var
NOEC
LOEC
ChV
Method
Overall Result
ChV [
659
44
54
210
9
10
0.720
10
10
0 736
10
10
0.626
10
10
0.763
1
10
10
0.795
10
10
0.784
660
44
56
220
1
8 11/ 7 98
7 61 / 7,38
24.3 / 24 3
2
9/12/2012
10
10
0.767
10
10
0.639
10
10
0.779
2
10
10
0.717
10
10
0.788
10
10
0 801
10
10
0 812
10
10
0 680
3
10
10
0.762
10
10
0 674
24 3
66
220
1130
<0 1
08
8
10
0.728
10
10
0.792
9
10
0.763
Survival
Critical
10
10
10
10
10
Calculated
16
18
18
14
18
0
8 24 / 8.03
7.81 / 7.14
24.5 / 24 8
0
8.20 / 7.94
7.74 7.05
24.4 / 24.8
1
9/10/2012
661
45
55
226
10
10
0.812
10
10
0.786
662
44
55
235
2
8.24 i 7 82
7 88 / 6.96
24 9 / 24.9
3
9/13/2012
10
10
0813
10
10
0 720
4
10
10
0.761
Stats
Cone.
0.5
1
2
4
8
4
8.26 / 8 15
7.63 / 7.44
24 8 / 25 0
5
8.30 / 8 00
7 72 / 7.19
24 8 / 25.4
6
8.25 / 7 98
7.68 / 6.73
24.4 / 25 1
% Eft. Repl.
[Control | Surviving #
Original #
Wt/original (mg)
22.8
59
215
1020
<0 1
0.7
24.5
62
218
1120
<0.1
1.1
2
8 18 / 8 00
7 80 / 6 83
24 9 / 24.9
6
8.24 / 7.89
7.65 / 6 ,-5
24.4 / 25 1
Growth
Critical Calculated
2.41 0.1936
2 41 0.1106
2.41 -0 4979
2 41 1 8533
2 41 -0.3319
1
8 16 / 8.02'
761 / 7 16
24 3 / 24 3
3
8.19 / 8.25
7 61 / 7 43
25 2 I 25.1
4
8 30 I 8.28'
7 64 / 7.47
24 8 / 25.0
5
8.35 / 8 1<
7 64 / 7 30
24 8 / 25.4
Environmental Sciences Branch
Division of Water Quality
N( DENR
1621 Mail .Service Center
Raleigh, 27699-1621
iritech, Inc.
* —
Signature'of pperator in Responsible Charge
Growth
Fl
Fl
8
>8
>8
Dunnet's
Survival
Fl
Fl
8
>8
>8
Steel's
Day
3
8.18 / 8.21
7 61 l 7 53
25.2 / 25 1
•••••••••••••••••••••••••••••••••••••••••tee
Client:Clayton # of Organisms per Chamber: 10 Start Date:9/11/12
NPDES#: NC0025453 Test Vessel Size: 400 ml End Date:9/18/12
Initiated by:
Date/Time Fed; 9/11/12 Temp, of Stock: Randomization:No
Date/Time Born: 9/10/12 # of Reps: 43:00 PM CT
Organism Source:Aquatox, Inc.
Transferred by:Fed by:
Date Time Initials Date Time 1 Initials Time 2 Initials Initial
s9/12Day 1 Day 0
Day 2 9/12 Day 1
Day 3 Day 2
Day 4 Day 3
*Day 5 Day 4
**Day 6 9/16 Day 5
9/17 Day 6
Test Termination Data:
*Initials/Signature
End Date:
End Time:
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
lll^______
*
Test Solution Volume: 250 ml
5f
9/13 3-sV,
°Clw
= Weekend, only 2 feedings needed
Time 3
9/13 (
9/14
9/15 9/16
9/17
9/11 10^)^
•
9/18/12
Time: 3 3S^:
Time: //3^
9/14 )U)Q^
9/15 H5a^-
Chemical and Physical Determinations
Concentration:control 1-2 2-3 4-5 5-6 6-7 Remarks
pH:
Final
D.O.:Initial
Final
Temp,:Initial
Final
Conductivity:
Hardness:
Alkalinity:
Concentration:0.5%Remarks
PH:Initial
Final
D.O.:
Temp.:
Conductivity:Initial
Concentration:1.0%Remarks
InitialpH:
Final
D.O.:Initial
Final
Temp.:Initial
Final
Conductivity:Initial
_________Final
Residual Chlorine:
Initial
Final
Residual Chlorine:
Initial
Final
Initial
Final
_______ Final <
Residual Chlorine: [ C1. j
Soft Synthetic Freshwater
Batch # :
Initial
Day
3-4
1-2
I 1-2
7- 3)
z 7-3
^4. 3
<^.17
3^"
i
Day
3-4
vr
JL1L
W
^3
74 3
2-3
7sy
7 j ?'
o-i
XaA,
7-76
677
^7^
^■1
7>l
cr/
zc./
ays
5-6
77/
0-i
Lil
29
gr.y
350
z z- /
7^'3
zd ■1
GO
.t73
7.3/
'7
7-7F
7/Q
aS r
6-7
g'.^
7?^
tTT
7-2-G»|G 7,7
^51
3-^7
4^. /
4-5
£-17
7. 43
7 ^7
? y y'
'2<r.o
7.6/
?5~7Z
^22-
7.36
6-7
773
-?Ho
2-3 ■ I
Q5S
7^2-
« c.1
^2
7^
AM
67?
23'?/
-7^
^0./
55-
MERITECH, INC.
ENVI RObJrviENT/XL LABORATORIES
xt Division of Water Technology and Dontrois, Inc.
Client: Clayton
1 est Organism: Pimephales promelas
4-5
^7 y 7
Tyr:
7 s .0
( o (s 0 '
M'T
M?
7.
-
0-1
£ y 0 ~w
MjL
2-9.^
77^
2-3
^23- m.
7-?7
4.97,
M
ns
^-g.j
S'
6|£~~
7. S
7
15-.Q
T-'Xic
^O. ! -
5-6
^■3!
y.ct
71- ,i
7.6/
M.S
2 S'- 77Ho~
77<r|773
Zp i I
74 ,9
J-J-9
23?
Time:
Time: f/
Day
I 3-4
W
2.M w
35?/
23^
Zj> 1
NPDES#: NC0025453 Start Date: 9/11/12
Analysl(s): mer, MR, ST End Date: 9/18/12
Chemical and Physical Determinations
Time:
9/18/12 Time:
Remarks
D.O.:
Temp.:
Conductivity:
Remarks
D.O.:
Temp.:
Conductivity:
Remarks
D.O.:
Temp.:
Conductivity:
Concentration:Remarks5-6 6-71-2
3 s ' </
Concentration:
pH:
NC0025453 Start Dale: 9/1 1/12
Analyst(s): mer, MR, ST End Date:
Concentration:
pH:
Concentration:
PH:
100%
pH:
Conductivity:
Residual Chlorine:
_______Hardness:
Alkalinity:
8.0%
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
4.0%
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
2.0%
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
5-6
HUE
S'.OZ
4-5
£
TXT
7.97
3 7.'7"
Day
3-4
Day
3-4
T -Fr
-Z7 6.
<d./
o-i
9r
~7.O2.
^3
i-O I
2-3
T77
~W
7. £ I
7k I
-7/
6-7
' ^'.Tj
7 <7^'
^77
747
3^^
/
35~.^-
2.rJ
4-5
■?. 54 Ztt
5-6
p.sr
^.il
7.3a
w
2-3
g..?c
7.&y
7 ?3 w
3*1 A
XU
X.S7
U-i
1-2
^-12-
7-^
7X7
7.70
U3
y-V 3
7 ci 7
Xo,/
6-7
~gTT
w
zr-/ ~
7^7
z-C',7
Page 2 of 2
Day
3-4
g. 17
7^
Client: Clayton NPDES#:
1 CSt Organism: Pimcphales promelas
1-2
s./c
7-X TTZF
4-5
-7 /.S' 7 6 7
2 77
Z33Z
0-1
ft-ay
S’. 0 3
7.^1
7./P
3cst
AM-
5-6
S3
5. tc
73^
7 2-6
'Z. S'. 7
77g'"
a±L_
ad.I
AT Q-/3-IX
11^2-3
*?. 77
•7,^7
6-S-3
~3o5~
XJO-
4-5
76 y
7. /.-
33lL.
3(3
7H3
zy^
~^7T
2M_IL 2FI
6-7
'yz'-l
7^
zy~r
5oy
7(7.)
1-2
7. i &
7t|3
2.^ -
^oj
C rxj \Z I F? f'j rv/| F NTAI L-ABORAIORIES
c.^f Cunlrtxls. /*-»<-
0-1
7.%
V.03
>4. A
uy
T.0• I
t0.\
sl____ Pay
2-3 | 3-4
7.3g
"HAD
Z () \ |______
/&Z
___21£
0-1
—
c o. I
-yy
Facility: Clayton Page: 1 of 2
NPDES #: NC0025453 Outfall:
Organism Pimephales promelas
by:
CONC # ORG.SURV %REP
333.53 35 (.53 /coA
O IO339.15 355 .93 |OUC 3 l-iB
850.3 3 10359.-35 /dD9.5.3 c :<coc
IO851 GA-'■'339 '35 3LW 0. iblD
C (S M5SS ,95A
IC\)/0359.05 9 Ate894, AB0.5%
3Hi 4 345,99 Q. 3 4 0 IdHc
Cj-llc'6^0,5°)-!, lc3I X:X .D
/o3^.54 19 9-.80 /Ct’4 '-4x 4 404A
10 /(X)389,933^44 3 oq c:-nciB1.0%
[00(04 549 8.CI c 9c ic
/eV|Ohint. 3(c-995.4-1 i 45D
WT. OF ORG.
(mg)
PAN WT.
(mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
MEAN
WT./ORG. (mg
C
o
O
PAN + ORG.
WT. (mg)
by: ST
ST
[00
Date of Test: 9/11/12
Initial weights taken on:
Final weights taken on: Lj IH- Its
45 f 4 4
MERlTECii, MC.
ENVIRONMENTAL LABORATORIES
A Vn-ision o! Water Technolacjy and ContmH. Inc
A
/0
ClaytonFacility:Page: 2 of 2
NPDES#: NC0025453 Outfall:
by; C3T Organism: Pimephales promelas
by: s
# ORG.SURV %CONG REP
/0-R5.59 !C09A
icDioB2.0%
10 let)4Mc;4 4.Q 940% . U.r-c
ro I(5j9.0cW.95D
4989 43TH.C3 C 9-XA
|(D/o'?79 09-9 Qi4,5/48B4.0%
/o98c 4c 0. COOc
5 O'C..H989 09D
~igQH -i-OUcC 10 C ■ -I .%loA
10990,93 9.lc98 c.4,50 C. COB8.0%
109 44984.59 998.90 4/4£c
(0999.%t 99-490 SOD
PAN WT.
(mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF ORG.
(mg)
MEAN
WT./0RG. (mg)
/m
/<£)
PAN + ORG.
WT. (mg)
MERSTECH,
ENVIRONMENTAL LABORATORIES
A DtK'tsion of W^itt^r Tftchrology ^nrf i'onr'Cils /ne
Date of Test: 9/11/12
Initial weights taken on: 99413
Final weights taken on: O'- V-( I'S
•9.9a
999.cc
RV
R5.59 9%,%
995 n
CCS0)
c.8ia
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Clayton NPDES#: NC0025453 9/11/12 Time: Start Date:
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST 9/18/12End Date:
Concentration Control
Day 0 Day 2 Day 3 Day 4 Day 5 Day6 Day 7
0uoRep#1
0 Ooo Rep #2
&0 0 c>o 0
0o 0o
0.5%Concentration
Day 0 Day 1 Day 2 Day 3 Day 7Day 4 Day 5
0 u o oRep #1
o 0ooGoGRep #2
u ooQC)12 Rep #3
600I0 O Rep #4
1.0%Concentration
Day 0 Day 3 Day 7Day 4 Day 5
0oCl oRep #1
0 0b O000
00o0
00 0 riRep #4
O
Day 1
0
Day 2
u
Day 1
0
Day 6
0
Day 6
0
Rep #2 0
0
Rep #4 Q
0Rep #3 O
o
Time: JI
0
o
Rep #3 0
Client: Clayton NPDES#: NC0025453 Start Date:9/11/12 Time:
ns©Test Organism: Pimephales promelas Analyst(s): mer, MR, ST 9/18/12 Time:End Date:
2.0%Concentration
Day 0 Day 2 Day A Day 5 Day 6
<5 c D Rep #1
0oo 0 o0Rep #2
0 0000O0uRep #3
0oo00
4.0%Concentration
Day 0 Day 1 Day 2 Day A Day 5 Day 6 Day 7
0 C-1oRep #1
o 0u Rep #2
b0 o L;Rep #3
0 ooo 00Rep #4
8.0%Concentration
Day 0 Day 2 Day 4 Day 6 Day 7
d) L2ARep #1
0 00oouu Rep #2
c 0 00 0Rep #3
0 o 0 Rep #4
0
MERITECH, INC.
Mortality Data: Chronic Fathead Test
6
Day 1
0
Day 3
0
Day 1
0
Day 3
0
Day 5
Day 7Day 3
0
0
0
Rep #4 &
Og0\S,CpMeritech Sample ID #:
Fax: [phone]
Web Site: www.meritech-labs.com
SAMPLE INFORMATION
Sample Site:
Sample Type: O Grab ® Composite # of containers:
Sampling Time: Start Date: 9 •" / Q ~ 2-0 I 2~Start Time AM PM
9 -//-2012End Date:End Time:AM PM
Signature:
TOXICITY TEST INFORMATION
IWC:%Test Concentrations (if multiple dilutions):
Comments:
Date:Time:3M- PM
Date: PM
Date:AM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature (°C):
Fed EX
X*
Relinquished by:
Received by. Date:4^Time:AM
Sample Temperatures (°C): I Sample Condition.
YELLOW = Client copy
'''
Relinquished by:
Received by>*’*:S^s^
Relinquished
Received by
Relinquished by:
Received by:
Relinquished by:
City:
County
Test Required: Chronic (7 days)
Q Acute (24-48 hours)
Test Organism: Ceriodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
Mysidopsis bahia (shrimp)
11:30 J±=^.
/O'./Z
2^--
2
'^0
Pl CU CO 3

WHITE = Laboratory copy
’ PO#:
NPDES#: NC
Phcne <7/^
Pipe #: g o l
zip
MERITECH, INC.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
Email: [e-mail]
CLIENT INFORMATION
(LLai^r^O k]_________________
■ g/ng.T ___/1'C /j
< okh 5
Client: y o
Contact Person:
Address:
/b'4O
Time: //‘rIO
Time: CO
/ 0-7 /
State: /[/6-
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED AND COVERED WITH ICE ***
Collector's Name; Print: rfbt
SHIPPING INFORMATION
^/U 2-
I // 2-
Method of Shipment: UPS Fed EX Meritech Pick-up
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery
SAMPLE RECEIVING (Laboratory Use Only)
PM
M
Meritech Sample ID #:
Fax: [phone]
Email: [e-mail] Web Site, www.meritech-labs.com
Client
Contact Person:
Address:
State:
SAMPLE INFORMATION
Sample Site:
O Grab C3 CompositeSample Type:2,# of containers:
Sampling Time:Start Date: Start Time: AM PM
End Date:End Time:AM PM
Signature.
TOXICITY TEST INFORMATION
Q Mysidopsis bahia (shrimp)
IWC: 2.%Test Concentrations (if multiple dilutions):
Comments:
//^
Timef'^jRelinquished by Date.
Received by Date: Time:
Relinquished by: 3Date: Time:AM
Received by: Date: Time: AM PM
Relinquished by: Date: Time. AM PM
Received by: Date: Time: AM PM
Relinquished by. Date: Time. AM PM
Received by: Date: Time: AM PM
Sample Temperature (°C).
iHsrDate:Time:AM PM
/ hl / /
WHITE - Laboratory copy YELLOW = Client copy
Test Required: Chronic (7 days)
l_J Acute (24-48 hours)
Test Organism: Ceriodaphnia dubia (water flea)
lX Pimephales promelas (fathead minnow)
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
A3
7//3/20/2-
"v/13 /)O
City
County. 7^4
A <7 I_____________________ _
T’ o . goz S’77 '//eo’o
5
It.
PO#.
NPDES#: NC .
Phone: 17) .
Pipe #: f
^P: Z? _____
. MERITECH, INC.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone [phone]
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by. /
Received -------------/y f
Sample Temperatures‘(°C)- 1 » i
CLIENT INFORMATION
<■’ T' Z7 / ^7
T/Zm J2 c. t r A
) c-^X a. ^2
Method of Shipment: UPS Fed EX Meritech Pick-up
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AND COVERED WITH ICE ***
Col lector's Name: Print: /.
AM PM
Xm? pm
SHIPPING INFORMATION
7// 3 //2-
. Sample Condition:
'M
£-Meritech Sample ID #
Fax: [phone]
Web Site: www.meritech-labs.com
CLIENT ^FORMATION
Client:PO#'
Contact Person:
State Zip:
Sample Site:
3Sample Type:# of containers.
-^7 <2.Sampling Time:Start Date:AM PM
2—End Date:PM
2?
TOXICITY TEST INFORMATiON
IWC:%Test Concentrations (if multiple dilutions).

Comments:
Date: Time.Z^S-Q PM
Date:Time:PM
/7/^Date: Time:PM
Date: Time: AM PM
Date Time: AM PM
Date l ime: AM PM
Date: Time- AM PM
Date: Time: AM PM
Sample Temperature (°C).
Fed EX
K*
VRelinquished by:
Received by: Date Time- AM
/ Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
Relinquished by: _<
Received by:
Relinquished by.x^
Received by:
Relinquished by:
Received by
Relinquished by:
Received by:
Test Organism: Ceriodaphnia dubia (water flea)
Pimephales prome/as (fathead minnow)
Mysidopsis bahia (shrimp)
Test Required: Chronic (7 days)
U Acute (24-48 hours)
Sample Temperatures
AH U'l
SAMPLE INFORMATION
Li Grab 53 Composite
/a m
npdes#- NC<g<pj?5V.5'3 ‘
Phone:
Pipe #. ef£>i
°iyw/id-
SAMPLE RECEIVING (Laboratory Use Only)
(°C):
MERITECH, INC.
S/oassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone [phone]
Email: [e-mail]
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
“ SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED AND COVERED WITH ICE
Collector's Name: Print: Signature:_____________________(T a
<=> ~f (2 / a^-
ijorperi /,
Address. ■ £0/ 9 / JOog
City:
County: ^9 A 41J /o tO
s+.
Method of Shipment: UPS Fed EX Meritech Pick-up
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery
AM
/&: ^m>
AM (
Start Time: /C> j 00
End Time, ff) '.fg" AM
SHIPPING INFORMATION
Z/z^b/^
T/t y /f
Transform:NO TRANSFORMATION
Shapiro - Wilk's Test for Normality-
Data PASS normality test (alpha = 0.01). Continue analysis.
Title:
File:
D =
W =
0.0471
0.8880
Critical W
W
clayton
clayton
= 0.8840 (alpha = 0.01 , N = 24)
= 0.9160 (alpha = 0.05 , N = 24)
Transform:NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated Bl statistic =5.0920 (p-value = 0.4048)
Data PASS Bl homogeneity test at 0.01 level. Continue analysis.
Title:
File:
5)
5)
Critical B = 15.0863
= 11.0705
clayton
clayton
(alpha = 0.01, df =
(alpha = 0.05, df =
Transform:NO TRANSFORMATION
ANOVA Table
SOURCE DF SS MS F
Between 5 0.0186 0.0037 1.4194
Within (Error)18 0.0471 0.0026
Total 23 0.0656
(p-value = 0.2646)
Critical F
Since F FAIL TO REJECT Ho: All equal (alpha. = 0.05)
(alpha = 0.01,
(alpha = 0.05,
df = 5,18)
df = 5,18)
Title:
File:
= 4 .2479
= 2.7729
clayton
clayton
< Critical F
Transform:NO TRANSFORMATION
Dunnett's Test TABLE 1 OF 2 Ho:ControlcTreatment
GROUP IDENTIFICATION T STAT
Dunnett critical value = 2.4100 (1 Tailed, alpha = 0.05,
Transform:NO TRANSFORMATION
Dunnett's Test TABLE 2 OF 2 Ho:Control<Treatment
GROUP IDENTIFICATION
Title:
File:
MIN SIG DIFF
(IN ORIG. UNITS)
MEAN CALCULATED IN
ORIGINAL UNITS
DIFFERENCE
FROM CONTROL
1
2
3
4
5
6
1
2
3
4
5
6
control
. 5
1
2
4
8
NUM OF
REPS
TRANSFORMED
MEAN
4
4
4
4
4
4
0.7588
0.7517
0.7548
0.7768
0.6917
0.7708
0.0871
0.0871
0.0871
0.0871
0.0871
0.7588
0.7517
0.7548
0.7768
0.6917
0.7708
% OF
CONTROL
11.5
11.5
11.5
11.5
11.5
0.1936
0.1106
-0.4979
1.8533
-0.3319
0.0070
0.0040
-0.0180
0.0670
-0.0120
SIG
0.05
control
.5
1
2
4
8
Title:
File:
clayton
clayton
clayton
clayton
df = 5,18)
Transform:NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
Data FAIL normality test (alpha = 0.01) . Try another transformation.
Warning
Title:
File:
D =
W =
clayton - survival
claytonsurv
0.0350
0.7221
The first three homogeneity tests are sensitive to non-normality
and should not be performed with this data as is.
Critical W
W
= 0.8840 (alpha = 0.01 , N = 24)
= 0.9160 (alpha = 0.05 , N = 24)
Transform:NO TRANSFORMATION
clayton - survival
claytonsurv
Title:
File:
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.
These two tests can not be performed because at least one group has
zero variance.
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 40 0 40
.5 39 1 40
TOTAL 79 1 80
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 40 0 40
1 40 0 40
TOTAL 80 0 80
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 40 0 40
2 40 0 40
Critical Fisher's value (40,40,40) (alpha=0.05) is 35.0. b value is 40.
Since b is greater than 35.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Critical Fisher's value (40,40,40) (alpha=0.05) is 35.0. b value is 39.
Since b is greater than 35.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
TOTAL 80 0 80
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 40 0 40
4 37 3 40
TOTAL 77 3 80
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 4 0 0 40
8 40 0 40
TOTAL 80 0 80
(alpha=0.05) is 35.0.
Summary of Fisher’s Exact Tests
NUMBER NUMBER SIG
Critical Fisher's value (40,40,40) (alpha=0.05) is 35.0. b value is 40.
Since b is greater than 35.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Critical Fisher's value (40,40,40) (alpha=0.05) is 35.0. b value is 40.
Since b is greater than 35.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Critical Fisher's value (40,40,40) (alpha=0.05) is 35.0. b value is 37.
Since b is greater than 35.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
GROUP IDENTIFICATION EXPOSED DEAD 0.05
40
40
40
40
40
40
0
1
0
0
3
0
1
2
3
4
5
CONTROL
. 5
1
2
4
8
Transform:NO TRANSFORMATION
Steel's Many-One Rank Test Ho: ControlcTreatment
GROUP IDENTIFICATION DF
Critical values are 1 tailed ( k 5 )
Title:
File:
clayton - survival
claytonsurv
MEAN IN
ORIGINAL UNITS
1
2
3
4
5
6
1.0000
0.9750
1.0000
1.0000
0.9250
1.0000
RANK
SUM
16.00
18.00
18.00
14.00
18.00
CRIT.
VALUE
10.00
10.00
10.00
10.00
10.00
4.00
4.00
4.00
4.00
4.00
SIG
0.05
control
. 5
1
2
4
8
“SERVICE""ENVIRONMENT”
June 21,2012
Re: Toxicity Results
To Whom It May Concern:
Please call me directly at 919-553-1536 if you have any questions.
Sincerely,
ames Warren, ORC
653 Highway 42 West • RO. Box S79 • Clayion, North Carolina 27520 • [phone] • Fax [phone]
VEHICLE MAINTENANCE
[phone]
Enclosed please find the Ceriodaphnia Pass/ Fail and Fat Head minnow toxicity results
lor the Little Creek Water Reclamation Facility.
WATER RECLAMATION
[phone]
PUBLIC WORKS
[phone]
ELECTRIC SERVICE
[phone]
TOWN OF CLAYTON
OPERATIONS CENTER
Environmental Science Branch
DWQ, NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 06/14/12
Facility: CLAYTON WWTP NPDES#: NC0025453 Pipe#: 001 County: JOHNSTON
Labor,ory
Comments: dilution water batch 13
-3.32% Reduction ** PASSED:
Work Order:
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 645 7 8 9 10 11 12
% Mortality Avg.Reprod.
# Young Produced 20 18 21 22 24 24 22 25 22 25 23 25
Adult (L)ive (D)ead L L L L L L L L L L L L
1 2 3 4 5 6 7 8 9 10 11 12
PASS FAIL
# Young Produced 26 23 20 23 25 24 26 21 23 24 2124 X
Check One
Adult (L)ive (D)ead L L L L L L L L L L L L
1st sample 1st sample 2nd sample
pH
Control 8.25 8.07 8.13 8.11 8.15 8.07
Treatment 2 8.27 8.12 8.13 8.18 8.11
1st
Grab DurationComp.
Sample 1 X 22.8 hrs
Sample 2 24.5 hrsX
46
7.72 7.50 7.75 7.49 7.21
Spec. Cond.(pmhos)185 1247 898
Treatment 2 7.85 7.51 7.75 7.57 7.70 7.27
Chlorine(mg/1)<0.1 <0.1
LC50/Acute Toxicity Test Sample temp, at receipt(°C)1.0 1.0
(Mortality expressed as %, combining replicates)
%%%%%%%%%%Concentration
%%%%%%%%%%
start/end
Control
PH D.O.
Organism Tested:Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT-1 (3/87)
Probit
Other High
Cone.
I
Note: Please
Complete This
Section Also
S
A
M
P
LC5 0 = %
95% Confidence Limits
% -- %
e
n
d
e
n
d
e
n
d
0.00
Treatment 2
Control CV
9.712%
0.00
Control
D
I
L
U
T
23.33
Treatment 2
22.58
Control
2nd
P/F
S
A
M
P
Mortality
start/end
% control orgs
producing 3rd
brood
100%
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Hardness(mg/1)D.O.
Control
Chronic Test Results
Calculated t = -0.891
Tabular t = 2.508
% Reduction = -3.32
s
t
a
t
1st sample
s
t
a
r
t
1st sample
Effluent %: 2.0%
TREATMENT 2 ORGANISMS
8.22
S
t
a
r
t
2nd sample
7.69
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
Method of Determination
Moving Average
Spearman Karber
Complete This For Either Test
Test Start Date: 06/06/12
Collection (Start) Date
Sample 1: 06/04/12 Sample 2: 06/06/12
Sample Type/Duration
also used:hard-46, cond-183
lerforming Test: MERITECH LABS, INC.
Si'gn^.ttire of Operator in ^Responsible Charge V
x
Signature of Laboratory Supervisor
rev. 11/95 (DUBIA ver. 4.41)
••••••••••••••••••••••••••••••••••••••••••st
Sample 1:Sample 2: - \
Sample 2
Batch #12-
LL C //L_/L 2 5
Total Produced
^0.1
1.0ZTo]LO
pH
Control
C
Sample
D.O.
Control
SampleLLL.L Lu L
Temp.
m ^31%) i ^\2s\Total Produced 21 Control
Comments: Sample
Sample
1
5
0
3
3
O
6
0
o
%
1
o
Meritech, Inc.
Mini Chronic Pass/Fail Test: Ceriodaphnia dubia
Date Start:
Time Start:
Adults Live / Dead
Day #7
# Young Produced
Adults Live / Dead
8 9 10 11 12
/? |/3p/ \/3\l/ |Z? |/^ .
c
Test Sample Organism Reproduction

| Effluent %:
1
0
7
6
U
8
O
11
o
12
O
3 - "
2®
6
O
9
o
10
o u
L
3 ^ 5 6 7 8 910 11 12
^|/2 \iz /I\if l/j? l// |/5T77T^
3
o
G/C?
c
9
Transfer Day
Hardness
(mg/L)
Spec. Cond.
(umhos/cm)
Chlorine
(mg/L)
Receipt Sample
Temp. fC)
Sample Information
100% pH
?.//
1st Sample
g’.iS'g'.o?
2nd Sample
o
U| U| U-l L-| u|
1 2 3 4 5 6 7
I 6
2
O
2
2nd Sample
7/7W?
Sample
2
Day #2
# Young Produced
Adults Live / Dead
1 2 3 4 5 6 7 8 9n ,0
1st Sample
7,1^-
7^ 7.57
initial Snal
^7
initial linal
2nd Sample
7 .7^ 7,^
7.7/ 7.57
initial ifirSi
2nd Sample <T
initial final
7.7<)7-Z7
initial find
2nd, Sample
initial final
initial final
o
H L
% 2,
8 9
ZpI o
4 5
o| O
Sample 1
11
2nd Sample
w \ni
8-i9
initial final ‘
1st Sample
41
-lA>
initial final
11 12
o o
Duration
2-2.5? hours
S hours
Incubator#:
Date End: /z
Time End:
Time: /£>.
Time;
&D.\ l$ |2I
Percent of Control producing third brood: )OD %
Transferred by: Fed by:
DayO
Day 1
Day 2
Day 3 ^/<V^
Day 4
Day5 2^^
Day 6
Terminated by:
Client CAcliA'Vq/^______ __
NPDES#: NC 00 2-5^53 Date /Ti
Dilution Water: Lake
Test Organism Source: Tray# 2^
Stirred / Aerated for D.O.: Y ^N)
Control Organism Reproduction
Day #2 1 2
# Young Produced
Adults Live / Dead
_______Day #5_____
# Young Produced
Adults Live / Dead
_______Day #7
# Young Produced
Adults Live 1 Dead
Pipe #: _cp I County: nZtd Date Start: // p
, lme of Culture Transfer^^p. / 0 Time Start: ^0^
Date / Time Neonates bom4 -.V-/2y l Renewal Date: (T/Z/T
_ Age of Neonates at Test Start: hours 2nd Renewal Date- XT
Randomized^/N Culture Tray Temp: °C Analyst^): mer^~R, ST Reviewed by:
Collection (Start) Dates?
//a-
4b"#4^
■
OlaOSioSMeritech Sample ID #.
Fax: [phone]
Email: txmrtech(a)bellsouth.net Web Site: www.meritech-labs.com
Client:
Contact Person:
Address.
/t/r.State:
Sample Site:
PM
End Time: PM
Signature:
TOXICITY TEST INFORMATION
IWC: 3 %Test Concentrations (if multiple dilutions):
Comments:
Relinquished by: Date: Time: PM
Date: Time;AM
Date:Time:AM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Sample Temperature (°C);
SAMPLE RECEIVING (Laboratory Use Only)
4,-5-/^Date.Time:AM
Sample Temperatures (°C):/ / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Received/oy,
Relinquished by:
Test Required: Chronic (7 days)
O Acute (24-48 hours)
City:
County:
Test Organism: Ceriodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
Mysidopsis bahla (shrimp)
■* CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
# of containers:
/S'
Start Time:
PO#:_____________________
NPDES#: NC^g
Phone:
Pipe #: €)€>)
Zip- ^75^
i—iW&nteC fc.X
'T^ujr'L *^ 3^,
l(p05
/2-cS
5
Relinquished by:
Received by: Uxpjuj TZn
LO
SAMPLE INFORMATION
Sample Type: Grab ® Composite
Sampling Time: Start Date:
End Date: (^1^'1 T-IT—
CLIENT INFORMATION
MERITECH, INC.
S/oassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AND COVERED WITH ICE *'
Collector’s Name: Print: /g ^4/ $iC (TTsttj Signature:
Method of Shipment: UPS Fed EX Meritech Pick-up
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
I 1.0
7g A A?
SHIPPING INFORMATION
6/ /(TL.
PI
-M'
Fax: [phone]
Email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
Zip:
SAMPLE INFORMATION
Sample Site:
Sample Type:# of containers:
Sampling Time:Start Date: Start Time:
End Date: End Time:
Signature:
TOXICITY TEST INFORMATION
Test Organism:
IWC:%Test Concentrations (if multiple dilutions):
Comments:
Date:Time:
Date:Time:AM
Date:AM
Date: AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Sample Temperature (°C):
Relinquished by:
Received by? Date: Time: AM
/ Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
Test Required: Chronic (7 days)
Acute (24-48 hours)
[XI Ceriodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
Mysidopsis bahia (shrimp)
-► CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
1^5 Composite
Sample Temperatures (°C):
PO#:
NPDES#: NO 002.^^^
Phone: Z? / y <3 5'
Pipe #: QO I
Method of Shipment: UPS
** Samples shipped on
Received by: —
Relinquished by: ~ ~ ------
Received by:
Relinquished by:^
Received by: ____________________
Relinquished by:_
Received by:
o
±2
/ (9 >'OO AM PM
/O'. 3 O 4M PM
MERITECH, INC.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
Grab
u SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
C Fed EX 0 Meritech Pick-up
Friday must be FedEx and must be clearly labeled for Saturday delivery
State. /J
1'0 / /. 0 I
'** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AND COVERED WITH ICE ***
Collector’s Name: Print: / e Signature:
Meritech Sample ID #: oGims
SHIPPING INFORMATION
6/ 7/^/^
</7 //2-
Z / 7 // 2^
Client: a 7* y^c> p)______ _______________
Contact Person: LcJclsr'C-h,
Address:
City:
County: S *0
Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 06/14/12
Facility: CLAYTON WWTP NPDES#: NC0025453 Pipe#: 001 County:JOHNSTON
Laboratory Performing Test: MERITECH LABS,INC.
Reduction: -3.32%CONTROL 2.0% Effluent
# Replicates 12 12
Female Live 12 12
Adult Male 0 0
Adult Dead 0 0
Adult Mortality 0.00%0.00%
# Neonates 271 280
Mean # Neonates 22.583 23.333
Standard Deviation 2.193 1.923
Coefficient of Variation 9.712%
Fisher's Exact Test
A = 12 B = 12 b = 12
a/A =1.00 b/B = 1.00
Success is:survival
Critical b value = 8
12 > 8
Test Passes I
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA
ORDERED OBSERVATIONS
i Group Neonates Centered i Group CenteredNeonates
18
20
20
21
21
21
22
22
22
23
23
23
-4.5833
-3.3333
-2.5833
-2.3333
-2.3333
-1.5833
-0.5833
-0.5833
-0.5833
-0.3333
-0.3333
-0.3333
C
E
E
E
C
C
E
C
C
C
E
E
23
24
24
24
24
24
25
25
25
25
26
26
0.4167
0.6667
0.6667
0.6667
1.4167
1.4167
1.6667
2.4167
2.4167
2.4167
2.6667
2.6667
1
2
3
4
5
6
7
8
9
10
11
12
C
E
C
E
E
C
C
C
C
E
E
E
13
14
15
16
17
18
19
20
21
22
23
24
The test concludes that the proportion of survival is not
significantly different for the control and the effluent groups.
a = 12
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.)
COEFFICIENTS AND DIFFERENCES
i x(n-i-l)x(i)a(i)x(n-i-l) - x(i)
1
V/X 88.3553
93.5833
Calculated W => 0.944 Critical W = 0.884
0.944 a 0.884
Test Passes!
F test for Homogeneity of Variance
Control variance 4.8106
F == 1.30
Effluent variance 3.6970
Numerator degrees of freedom: 11 Denominator degrees of freedom: 11
Critical F = 5.32
1.3 0 s 5.3 2 =► The Test the variances of the two groupsPASSES,
are significantly the same, homogeneous.
1
2
3
4
5
6
7
8
9
10
11
12
2.6667
2.6667
2.4167
2.4167
2.4167
1.6667
1.4167
1.4167
0.6667
0.6667
0.6667
0.4167
-4.5833
-3.3333
-2.5833
-2.3333
-2.3333
-1.5833
-0.5833
-0.5833
-0.5833
-0.3333
-0.3333
-0.3333
0.4493
0.3098
0.2554
0.2145
0.1807
0.1512
0.1245
0.0997
0.0764
0.0539
0.0321
0.0107
7.2500
6.0000
5.0000
4.7500
4.7500
3.2500
2.0000
2.0000
1.2500
1.0000
1.0000
0.7500
The reproduction data is normally distributed
evaluated at a 99% confidence interval.
EQUAL VARIANCE t TEST
22.6 23.3
t = = -0.891
0.842
Degrees of freedom = 22
Critical t = 2.508
-0.891 < 2.508
Chronic Test PASSES
Test passed. There is not a significant difference in reproduction
between the Control and the effluent evaluated at a 99% confidence interval.
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date:6/18/2012
Facility: Clayton County: JohnstonNPDES # NCOO 25453 Pipe #: 001
Comments |
x
Sign;
x
Signature of Laboratory Supervisor
MAIL ORIGINAL TO:
Test Initiation Date/Time 6/5/2012 4.20 PM Avg Wt/Surv. Control 0.746 Test Organisms
% Survival 100.0
Hatch Date:6/4/12Avg Wt (mg)0.746
Hatch Time: 3:00 pm CT100.0% Survival
Avg Wt (mg)0.696
1 % Survival 100.0
0.729Avg Wt (mg)
2 % Survival:100.0
0.722Avg Wt (mg)
[4 100.0% Survival
Avg Wt (mg)0.713
% Survival[J897.5
]Avg Wt (mg)[0.778
J
nu/n Com-, at c z-f/nxi
Dilution H2O Batch#
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
J Surviving #
Original #
Wt/original (mg)
r Cultured In-House
F Outside Supplier
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp, at Receipt (“C)
0.5 | Surviving #
Original #
Wt/original (mg)
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
I Surviving #
Original #
Wt/original (mg)
~] Surviving #
Original #
Wt/original (mg)
Surviving #
Original #
Wt/original (mg)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Normal
Hom. Var.
NOEC
LOEC
ChV
Method
Environmental Sciences Brunch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
637
48
56
220
0
8.31 / 8.13
7.49 / 6.91
25.3 / 25.1
10
10
0.763
10
10
0.766
10
10
0.782
10
10
0.776
10
10
0.742
638
48
55
212
639
46
58
204
1
8.29 ! 7.75
7.60 / 6.62
24,8 i 25 4 24 7 i 24.6
1
8.30 / 7.91
7.60 / 6.36
24.8 / 25.4
2
6/6/2012
10
10
0.717
10
10
0.811
10
10
0.770
10
10
0 750
2
10
10
0.766
640
46
56
206
9
10
0.774
10
10
0.749
10
10
0 690
10
10
0.646
3
10
10
0.822
10
10
0.724
10
10
0.762
10
10
0 706
4
10
10
0.719
Stats
Cone.
0.5
1
2
4
8
Survival
Critical
10
io
10
10
10
5
8.22 / 7.85
7.66 / 6.68
24.7 / 25.5
Calculated
18
18
18
18
16
6
8.26 / 7.77
7.57 / 6.31
24.8 / 25.4
Calculated
1.3673
0.4715
0.6534
0.9093
-0.8622
% Eff. Repl.
| Control [ Surviving #
Original #
Wt/original (mg)
o
8.29 / 8.07
7,52 / 6.83
25.3 / 25.1
1
6/4/2012
1
10
10
0.678
23.2
62
260
1308
<0.1
1.0
10
10
0.653
2
8 10/ 7.92
7.51 / 6.75
2
8 12 / 7.89
7.57 / 6.66
24.7 / 24.6
3
6/7/2012
10
10
0.657
23.5
74
123
829
<0.1
0.8
10
10
0.690
10
10
0.620
4
8.30 / 8.04
7.66 I 7.06
25.3 / 25.5
6
8.26 / 8.00
7,56 / 6 58
24.8 / 25.4
24,5
72
145
915
<0.1
1 0
La^^^______________
attjrexlt Operator in Responsible Charge
Growth
Critical
2.41
2.41
2.41
2.41
2.41
Growth
Fli
Fl:
8
>8
>8
Dunnet's
Survival
ru
rh
8
>8
>8
Steel's
5
8.22 / 7.93
7.66 / 6.77
24.7 / 25.5
3
8.19 / 8.27
7.56 / 7.50
24.4 / 24.7
4
8.34 I 8.05
7.74 I 7.15
25.3 / 25.5
Day
3 ~
8 18 I 8.19
7 57 / 7.45
24 4 / 24.7
Overall Result
ChV | >8
•••••••••••••••••••••••••••••••••••••••••••a
ClaytonClient:# of Organisms per Chamber: 10 Start Date:6/5/12 Time:
NPDES #: NC0025453 Test Vessel Size: 400 ml End Date:6/12/12 Time:
Initiated by:.Test Solution Volume: 250 ml
Temp, of Stock: Date/Time Fed: 6/5/12 Randomization:Yes No
Date/Time Born: 6/4/12 # of Reps: 43:00 PM CT Incubator #:
Organism Source:Aquatox, Inc.
Transferred by:Fed by:
Date Time Initials Date Time 1 Initials Time 2 Initials Time 3 Initial
6/6Day 1 6/5Day 0
6/7Day 2
Day 3
Day 4 6/8Day 3
Day 5 Day 4
6/11 ^57? **Day 6
6/11 Day 6
Test Termination Data:
Initials/Signature:
End Date:
End Time:
6/8
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
{
*
°C
6/12/12
= Weekend, only 2 feedings needed
6/9
6/10 IW-"'
Day 1 6/6 /4^-
Day2 _6/7 5~^pr
Jj-l-Opv- _ /rn il-l^Ar /T-
_6Z1_ X___icCCptt- *
Day 5 6/10 735^^ — 3/^
Chemical and Physical Determinations
0-1 1-2 2-3 4-5 5-6 6-7 Remarks
pH:
D.O.:
Temp.:
Conductivity:
4-5 Remarks
D.O.:
Temp.:
Conductivity:
Remarks
D.O.;
Temp.:
Conductivity:
Concentration:
PH:
6-7
Day
3-4
Client: Clayton NPDES#: NC0025453
Test Organism: Plmephales promelas
Concentration:
PH:
Concentration: control
Soft Synthetic Freshwater
Batch # :
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
_______Hardness:
Alkalinity:
0.5%
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
X/0
7-9^
757
6.
2^7
2 7^
7.
766
1%
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
7.?r
7-670
6-6 2-
U/X
255/
z/uT
2 6 J"
2-3
7
7-57
6.S-2
2^7
276
232
2 /
I ~w
ST
Day
3-4
MO
7.^
77?
f
0-1
y,
7 r/
760
2 $23
27/
73/
2a |
332
g.67
7-rz-
•2-7. 31
I
-
3 6 -
1-2
In.
24-
Ml
-^75
^0. /
5-6
' 1.^
?r.s
’ 727
'oTT
/
zo/
.Ffc
6-7
y.zG
7 7^
ZS.l/
7Bo>
5Lo\
<0. '
1-2
7.?r
7.62
2M-y
27‘Z
2-33
74
2- O. I
7.PT
MM
dA'i1
-mT
ZK).,)
-
fr.7>9
3(o(g
27 3______
/73, zzy
^<9 J
—
4-5
TH
7 or
mT
T5-
zo. I
Day
3-4 ___
g-. /s’W^t g.iq
2J2_
TT
t/6
20-/
7.72
(q-ST
27 ?
2^6
23 2
^r/
4^. [
(n
TH
MM
^-o. 1
5-6
^22
z-y?
G?. 4 2
z.r.6
zig'
34*r
<65-/
0-1
£■07
(g 6'2
2X3
zf /
2XT
zo. /
MERITECH. II\IC.
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls. Inc.
2-3
7.xX 8~, |5
7 d"2
W-
Z-iO
^o. '
Time: ^2Z>/T-
Time: 4532%^
'SS/M
TO
7^. /
Start Date: 6/5/12
Analyst(s): mer, MR, ST End Date: 6/12/12
Chemical and Physical Determinations
Remarks
D.O.:
Temp.:
Conductivity:
^4
Remarks
D.O.:
Temp.:
Conductivity:
6-7 Remarks
D.O.:
Temp.:
Conductivity:
^0. I
Concentration:4-5 6-7 Remarks
/Z5
Concentration:
pH:
Concentration:
~ pH:'
1-2
Day
3-4
Time:
Time:
NPDES#: NC0025453 Start Date: 6/5/12
Analyst(s): mer, MR, ST End Date: 6/12/12
4%
Initial
"Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
Concentration:
PH:
2%
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
5-6
7 <13
7 b io
(o.yy
6-7
8%
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
z/%rcr-
ENVIRONMENTAL IW3ORATORIES
o/v/,,0^. o/ uv>r-r n,cJl„o(ooy Cor.r,o«. ,r<c.
2-3
^11
783'
~>.ST
^<7
^.7
oFS
z a I
5-6
3.37
6^7
2,.r V
zzZ __
^0-1
100%
PH:
Conductivity:
Residual Chlorine:
Hardness:
Alkalinity:
Day
3-4
f < ?JO
XiT o.r?
mr
^-¥7
1237
4(9. /
A/l. (
776
'-7,57
•z/z' y
>yi Tn) 1
5-6
P.2-(
\jr.5’
7.38'
¥lo7
O I
TUP
Io 3 (5
7.
?¥ 7 w
2-3zsurxzE
7. ^7
7
7t/-b
279_
GW<^7
Day
3-4
g.zo
8,30
311
3). /
4-5
3I4n
,/g.Q3
7¥5'I7.(q¥
7-o^
1-2
f.zy
7-^
"ZtoO
(a
40-1
0-1
?, 3Z
Zjt'
7222
27/
6-7
y.z-?
7-W
27-y
7j~¥
33A
6-3? my
zr.7
-v-H
2<5./
4-5
S3T
7-Q5' w
7457,
777
z-p. 1
| 52m \ ~~
^,7 i
2-3
7.13
75b
<
ACJ- I
4-5
y.o?-
7.1^
Client: Clayton
1 est Organism: Pimephales promelas
_____ Day
2-3 | 3-4~
^o./
Page 2 of 2
7,33 Tyv-
yT7
'7¥j '
0-1
7-SD
(p.Q2-
2.0 I i
WT W
40 I
1-2
■■S’.
7^4-
G.
21Z-
GlfXp
3^
/
0-1
7 3/
¥|3 797
25-1
25
o-l 1-2
^3/
130^
20 /
Facility: Clayton Page: 1 of 2
NPDES#: NC0025453 Outfall:
Organism: Pimephales promelas
SURV %# ORG.CONG REP
ftO23b 15 Cd .32^,9 93 /oA
O ^33.99 io^91.3^3.1 pinB
^43.49 0.W I.CX)W.5\2.3ac
C) .3133.Asm. 3ci339.1 \/oD
34413k> SSi-BSC G.-W /cP3.2a |OA
93343,In.53 0,.CoS'SR4I.M5 io 160B0.50%
S4l -0)3 (0 G.io.sa-343.59 b.53 ICOc
co 0-49C2.33.53 344.93D
fpB44S5 0 334,440 .Oc f£0A
234. IS 543.35 3 30 ICl)0.330B1.0%
3.49c
430.03 3.3in.Cn 5.D
PAN WT.
(mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF ORG.
(mg)
MEAN
WT./ORG. (mg)
c o
O
PAN + ORG.
WT. (mg)
0.344
Ca lC)9-C> I
WiERITECH, /A/O
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls, Inc.
0.3(n(o
St
|0
(CD
(OS
4.9 o
3 44
Date of Test: 6/5/12
Initial weights taken on: by:
Final weights taken on: |9> by: 'OSH
ClaytonFacility:Page: 2 of 2
NPDES#: NC0025453 Outfall:
Organism: Pimephales promelas
#ORG.SURV %CONG REP
^3^^, Si /o |A
(0^.50 0350B2%
(0 /60(o Ao O.lrAOc
/60ODOOFtOo/OD
A39.cn §4(0,30 /6otoA-A
IO>105. H 0.0 3B4%
/(>>|O351.03 3 A A. 04 Co.4lo 0 v LsHCjc
i'oO0304|05.04D
|O /GO854. oa OfcA
10 /4)AM ACMi. 55 0, 1A 11B8%
530 .op 033,14 035433-4c
sM. 431340.55 To AD
PAN WT.
(mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF 0RG.
(mg)
MEAN
WT./0RG. (mg)
PAN + ORG.
WT. (mg)
MERITECH, INC.
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls. Inc.
SM.AA 3454o>
341.40 W,4o
833.0C0 MA.ACn
MG,
COCgQI i'^
95
Date of Test: 6/5/12
Initial weights taken on: (p~ lo" by:
Final weights taken on: by: SV
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Clayton NPDES#: NC0025453 6/5/12Start Date:
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST 6/12/12 Time: End Date:
Concentration Control
Day 0 Day 1 Day 2 Day 3 Day 4 Day 6
0 0Rep#1
ooRep #2
0 Rep #3
O '0000Rep #4
0.50%Concentration
Day 0 Day 1 Day 3 Day 6 Day 7Day 4 Day 5
Rep #1
0&
Rep #2
0 c)o aRep #3
0 0 06/Rep #4
1.0%Concentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6
Rep#1
c)Rep #2
0Rep #3
dRep #4
0
0
Day 7
0
Day 7
0
Day 2
0
Time:
0
0
o
0
Day 5
0
o
0
0
0
0
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Clayton NPDES#: NC0025453 6/5/12 Time:Start Date;
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST 6/12/12 Time:End Date:
2%Concentration
Day 0 Day 1 Day 2 Day 7Day 3 Day 4 Day 6
0 coRep #1
0 c<2)Rep #2
0 0 6Rep #3
0 0 0 Rep #4 J
4%Concentration
Day 0 Day 1 Day 3 Day 7Day 4
6Rep #1
oRep #2 (Q
6 O Rep #3
0o 0 aRep #4
8%Concentration
Day 0 Day 1 Day 3 Day 4 Day 5 Day 6 Day 7
0c
(J000 Rep #2
Rep #3
0Rep #4
Day 2
6
Day 5
0
Day 6
0
Day 2
0
o
Day 5
0
0
0
o
Rep #1
OG05I05Meritech Sample ID #:
CLIENT INFORMATION
3/;
State:
Sample Site:
A\1 PM
End Time: PM
Signature:
TOXICITY TEST INFORMATION
IWC: Q %Test Concentrations (if multiple dilutions):
Comments:
IODODate:Time:> PM
Date:Time: AM
Date:Time:AM 'M'
Date: Time. AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM '
Sample Temperature (°C):
^-5-/2^Date:Time:AM PM
Sample Temperatures (°C):/ / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
Relinquished by;
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received/oy>
Test Required: Chronic (7 days)
Acute (24-48 hours)
Client:
Contact Person:
Address:
City:
County:
Test Organism: Ceriodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
Mysidopsis bahia (shrimp)
# of containers:
H: J S~
Start Time:
PO#:
NPDES#: NC^g
Phone/*?3-3- 3 '
Pipe #: Qo)
aP ^75^ . ■
/AO 5
MERITECH, INC.
Bloansay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
Email: txmrtech(5)bellsouth.net
/(pO5
Relinquished by: NlKttnd
Received by:
I.O / /.o
SAMPLE INFORMATION
/A'A A / ___
Sample Type: Grab ® Composite
Fax: [phone]
Web Site: www.meritech-labs.com
Sampling Time: Start Date: Q/^/
End Date: 2. 0/2—
'/oq o
Method of Shipment: UPS Fed EX Meritech Pick-up
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
SAMPLE RECEIVING (Laboratory Use Only)
•* CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AND COVERED WITH ICE ***
Collector's Name: Print: / c ;j/ fa f Signature: /mA
<7 / u
SHIPPING INFORMATION
~O-
6/ ^//2-
6/ ^ /( ZL.
3ct IA ets r ,
ZBoX '
"AoCfyff /-OLA
CLIENT INFORMATION
State:Zip:
SAMPLE INFORMATION
# of containers:
Sampling Time: Start Date: Start Time:
End Date:
TOXICITY TEST INFORMATION
Test Organism:
*WC:_JL %
Comments:
Date:Time:
Date:Time:AM
Date: Time:AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: PMAM
Date: Time: AM PM
Sample Temperature (°C):
C -7'Date:Time: AM
1.0 ,Sample Temperatures (°C):/. 0 / ! Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
Relinquished by:
Received by.-
® Ceriodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
Q Mysidopsis bahla (shrimp)
Test Concentrations (if multiple dilutions):
Test Required: Chronic (7 days)
Acute (24-48 hours)
^/?/go/
Fax: [phone]
Web Site: www.meritech-labs.com
Relinquished by:
Received by:
Relinquished by: /
Received by:
Relinquished by:
Received by:
Relinquished by:
•Received by:
PO#:;
NPDES#: NC
Phone: (cj t -/
Pipe #: QQ [
^7^
MERITECH, INC.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
Email: [e-mail]
6/
6/'] /12^
(? / 7 / / 2-
SHIPPING INFORMATION
Date:
Method of Shipment UPS Fed EX Meritech Pick-up
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
r SAMPLE RECEIVING (Laboratory Use Only)
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10.00 AM IF AT ALL POSSIBLE
SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED AND COVERED WITH ICE ***
Collectors Nam.: Print:Signature:
am pm
End Time: /0‘. 3 O A^M PM
Meritech Sample ID #: ooms
Client. 7^ a
Contact Person: 4<>M-e5 //aF-HpC
Address:? 7/000 7bIZt
County: 7~o A *n 5 *-0
Sample Site:
Sample Type: Grab Composite
Time: /:
/6 / 5
AM PM
AM <PM?
O^ov-^Meritech Sample ID #:
Web Site: www.meritech-labs.com
Address:
Sample Site:
3# of containers:
Sampling Time: Start Date:
End Date:
Signature:
IWC: %
Comments:
Date:
Date:
Date:AM M
Date: AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Sample Temperature (°C);
Date:AM 'M
/ /
WHITE = Laboratory copy YELLOW = Client copy
Client:
Contact Person:
City:
County:
Test Organism: Ceriodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions):
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Test Required: Chronic (7 days)
Acute (24-48 hours)
State:
SAMPLE INFORMATION
® Composite
Relinquished by:
Received by:fl'
Sample Temperatures (°C):
Sample Type: Grab
PM
PM
PO#:
NPDES#: NO
phone:
Pipe #: QQ I
Z'P: _______
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AND COVERED WITH ICE ***
CoUector-sName: Print: Slgnature:,
TOXICITY TEST INFORMATION
a/rjrPA __r
3OK 7 /Me J),
Time:
Time: /Q y
Time: / O
Time:
Time:
Sample Condition: J
Method of Shipment: UPS
** Samples shipped on
CLIENT INFORMATION
>UX~Zz7
MERITECH, INC.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: [e-mail]
SHIPPING INFORMATION
6/Z //
6/% / /
&/ ^7IStart Time: AM__ PM
6 7^orx- End Time: /fi lOO AM PM
G Fed EX Q Meritech Pick-up
Friday must be FedEx and must be clearly labeled for Saturday delivery ’■*
SAMPLE RECEIVING (Laboratory Use Only)
Transform:NO TRANSFORMATION
Shapiro - Wilk's Test for Normality-
Data PASS normality test (alpha = 0.01). Continue analysis.
Title:
File:
D =
W =
0.0496
0.9875
Critical W
W
= 0.8840 (alpha = 0.01 , N = 24)
= 0.9160 (alpha = 0.05 , N = 24)
clayton
clayton
Transform:NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated Bl statistic =4.7564 (p-value = 0.4463)
Data PASS Bl homogeneity test at 0.01 level.Continue analysis.
Critical B = 15.0863
= 11.0705
Title:
File:
5)
5)
clayton
clayton
(alpha = 0.01, df =
(alpha = 0.05, df =
NO TRANSFORMATIONTransform:
ANOVA Table
FMSSOURCEDFSS
1.20570.00330.0166Between5
Within (Error)0.00280.049618
Total 23 0.0662
(p-value = 0.3461)
Critical F
Ho: All equal (alpha = 0.05)Since F < Critical F FAIL TO REJECT
Title:
File:
= 4.2479
= 2.7729
(alpha = 0.01, df = 5,18)
(alpha = 0.05, df = 5,18)
clayton
clayton
Transform;NO TRANSFORMATION
Dunnett's Test Ho:ControlcTreatmentTABLE 1 OF 2
GROUP IDENTIFICATION T STAT
Dunnett critical value = 2.4100 (1 Tailed, alpha = 0.05, df = 5,18)
NO TRANSFORMATIONTransform:
Ho:ControlcTreatmentDunnett's Test TABLE 2 OF 2
GROUP IDENTIFICATION
Title:
File:
Title:
File:
1
2
3
4
5
6
NUM OF
REPS
TRANSFORMED
MEAN
4
4
4
4
4
4
0.7463
0.6955
0.7288
0.7220
0.7125
0.7782
MIN SIG DIFF
(IN ORIG. UNITS)
0.0895
0.0895
0.0895
0.0895
0.0895
MEAN CALCULATED IN
ORIGINAL UNITS
0.7463
0.6955
0.7288
0.7220
0.7125
0.7782
% OF
CONTROL
12.0
12.0
12.0
12.0
12.0
DIFFERENCE
FROM CONTROL
1.3673
0.4715
0.6534
0.9093
-0.8622
0.0508
0.0175
0.0242
0.0337
-0.0320
SIG
0.05
1
2
3
4
5
6
control
0.5%
1%
2%
4%
8%
control
0.5%
1%
2%
4%
8%
clayton
clayton
clayton
clayton
Transform:NO TRANSFORMATION
Title:
File:
clayton survival
claytonsurv
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 40 0 40
0.5%40 0 40
TOTAL 80 0 80
Critical Fisher's value (40,40,40) (alpha=0.05) is 35.0.
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 40 0 40
1%40 0 40
TOTAL 80 0 80
(alpha=0.05) is 35.0.
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 40 0 40
2%40 0 40
Critical Fisher's value (40,40,40) (alpha=0.05) is 35.0. b value is 40.
Since b is greater than 35.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
... b value is 40.
Since b is greater than 35.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
TOTAL 80 0 80
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 40 400
4%40 0 40
TOTAL 80 0 80
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 40 0 40
8%39 401
TOTAL 79 1 80
Summary of Fisher's Exact Tests
NUMBER NUMBER SIG
Critical Fisher's value (40,40,40) (alpha=0.05) is 35.0. b value is 39.
Since b is greater than 35.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Critical Fisher's value (40,40,40) (alpha=0.05) is 35.0. b value is 40.
Since b is greater than 35.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Critical Fisher's value (40,40,40) (alpha=0.05) is 35.0. b value is 40.
Since b is greater than 35.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
GROUP IDENTIFICATION 0.05EXPOSEDDEAD
1
2
3
4
5
40
40
40
40
40
40
0
0
0
0
0
1
CONTROL
0.5%
1%
2%
4%
8%
Transform:NO TRANSFORMATION
Steel's Many-One Rank Test Ho: ControlcTreatment
GROUP IDENTIFICATION DF
Critical values are 1 tailed ( k = 5 )
Title:
File:
clayton survival
claytonsurv
1
2
3
4
5
6
MEAN IN
ORIGINAL UNITS
1.0000
1.0000
1.0000
1.0000
1.0000
0.9750
RANK
SUM
18.00
18.00
18.00
18.00
16.00
CRIT.
VALUE
10.00
10.00
10.00
10.00
10.00
4.00
4.00
4.00
4.00
4.00
SIG
0.05
control
0.5%
1%
2%
4%
8%
Transform:NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning
Title:
File:
D =
W =
clayton survival
claytonsurv
0.0075
0.4651
Critical W
W
The first three homogeneity tests are sensitive to non-normality
and should not be performed with this data as is.
= 0.8840 (alpha = 0.01 , N = 24)
= 0.9160 (alpha = 0.05 , N = 24)
“SERVICE"“ENVIRONMENT"
March 29, 2012
Re: Toxicity Results
To Whom It May Concern:
Please call me directly at 919-553-1536 if you have any questions.
Sincerely,
es Warren, ORC
653 Highway 42 West • P.O. Box 879 • Clayton, North Carolina 27520 • [phone] • Fax [phone]
Enclosed please find the Ceriodaphnia Pass/ Fail and Fat Head minnow toxicity results
for the Little Creek Water Reclamation Facility.
TOWN OF CLAYTON
OPERATIONS CENTER
VEHICLE MAINTENANCE
[phone]
ELECTRIC SERVICE
(9)9)553-1530
WATER RECLAMATION
[phone]
PUBLIC WORKS
[phone]
Environmental Science Branch
DWQ, NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 03/22/12
Facility: CLAYTON WWTP NPDES#: NC0025453 Pipe#: 001 County: JOHNSTON
Comments: dilution water batch
mat-and 942 used.Cnarge 940,941,
Work Order:
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
% Mortality Avg.Reprod.
# Young Produced 18 19 17 17 21 17 20 20 17 13 20 20
Adult (L)ive (D)ead L L L L L L L L L L L L
1 2 3 4 5 6 7 8 9 10 11 12
PASS FAIL
# Young Produced 17 22 21 17 20 19 19 20 15 20 19 18 X
Check One
Adult (L)ive (D)ead L L L L L L L L L L LL
1st sample 2nd sample
PH
Control 8.04 7.98 7.98 8.04 7.91
Treatment 2 8.02 8.00 8.04 7.97 8.04 8.02
1st
Grab Comp.
Sample 1 22.5 hrsX
Sample 2 24.5 hrsX
Hardness(mg/1)44
7.64 7.43 7.70 7.32 7.50 7.25
Spec. Cond.(pmhos)167 1212 1203
Treatment 2 7.51 7.35 7.78 7.45 7.50 7.10
Chlorine(mg/1)<0.1 <0.1
LC50/Acute Toxicity Test Sample temp, at receipt(°C)1.8 0.5
(Mortality expressed as combining replicates)
% %%%%% %%Concentration%%
%%%%%%%% %%
start/end
Control
PH
Organism Tested:Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT-1 (3/87)rev.
L
D.O.
1st sample
8.07
Effluent %: 2.0%
TREATMENT 2 ORGANISMS
Moving Average
Spearman Karber
Method of Determination
Probit
Other
Chronic Test Results
Calculated t = -0.785
Tabular t = 2.508
% Reduction = -3.65
I
Note: Please
Complete This
Section Also
18.92
Treatment 2
S
A
M
P
LC50 = %
95% Confidence Limits
% %
0.00
Treatment 2
Control CV
12.169%
0.00
Control
High
Cone.
D
I
L
U
T
18.25
Control
2nd
P/F
S
A
M
P
e
n
d
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
e
n
d
e
n
d
Mortality
start/end
% control orgs
producing 3rd
brood
100%
D.O.
Control
s
t
a
r
t
1st sample
s
t
a
r
t
1st sample
s
t
a
r
t
2nd sample
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
e of Operator in Responsible
x „ -
Signature of Laboratory Supervisor
X
S
Complete This For Either Test
Test Start Date: 03/14/12
Collection (Start) Date
Sample 1: 03/12/12 Sample 2: 03/14/12
Sample Type/Duration
Duration
11/95 (DUBIA ver. 4.41)
* PASSED: -3.65% Reduction *
Laboratory Performing Test: MERITECH LABS, INC.
••••••••••••••••••••••••••••••••••••••••••st
Pipe#: eoi
3/zZ
z-
10
Sample 1:Sample 2:O
Batch #
2
Total Produced
:o-l <O,i
r J. 3
PH
Control
Sample
D.O.
Control
Sample
Temp.
FQ'i pi i p iaOmimowluiTotal Produced
Control
Comments:
Sample
1
O
2
C>
9
o
5
Duration
hours
hours
Age of
Randomized.^ Y)/ N
Sampl« Sample
" I 2
Zz-S
# Young Produced
Adults Live / Dead
Day #5
# Young Produced
Adults Live / Dead
pay #7
# Young Produced
Adults Live / Dead
Sample 1
Sample 2
Day #2
# Young Produced
Adults Live / Dead
Day #5
# Young Produced
Adults Live / Dead
Day #7
# Young Produced
Adults Live / Dead
10
O
6
o
I
0Transfer Day
Hardness
(mg/L)
Spec. Cond.
(umhos/cm)
Chlorine
(mg/t)
Receipt Sample
Temp. fC)
100% pH
L
12
Sample Information
G/C?
C
9
u ci u
10/ 11/ 12
12
L
6 7 8
~d e>l p c
1st Sample
2^
7^
initial ?Inai
2nd Sample
A
initial final
2nd Sample
7^ [ft'
7.^
initial ?!nai
1st Sample
7.98/
initial final
2nd Sample
75° I?. 25
7^7.10
initial finai
2nd Sample
25.0
Initial fuTai
2nd Sample
TA I
?:o/R.O2
initial (mai
1st Sample
2£b
initial final
7 8
o~| o
C- L.
3 4
ol o
ClE
ivi'cru-iLTLl lI l r;_
1 2 3i 4 5 6 7 8 9.- 10 11
/6 I I y 7TrWTTn-
J^iZtZZLu I u I c I l I d 'll I (' ru
2nd Sample
Ww
initial |f\nai
11 12
El o
Meritech, Inc.
Mini ChronicPass/FailTest: Ceriodaphnia dubia
County: Date Start:
Date / Time of Culture Transfert^a J Time Start-
Test Organism Source: Tray#
Stirred / Aerated for P.O.: Y ($)
^Control Organism Reproduction
Day #2 1 2
Elo
~U-E
1
3 //4?,
^/jc
mer, MR, ST
4 5
o | o_
u.
\^\ A| A;l-| L | < | ^ | t | [^ | C L U | E L,
— i 2 -3-> 1 6 7. j 9 10 11 12
xJ-TlI l h J f. i_ j 11 | l_ / | l|i_
MLWima/1 i 7l^ l^l / TPjfenWI
Percent of Control producing%rJd Sood: %
Test Sample Organism Reproduction
Effluent %: ~|
Client C.AzXsxAtoA
NPDES #: NC oALAq S3
Dilution Water Lake Reidsvill.
Incubator#:
Date End:
Time End:
Time:
Time:
--------j-----J-------Q'HLAft, Time Start:
Date / Time Neonates bornSAj-;?/ '7:^ 1st Renewal Date:
Age of donates at Test Start: jX . hours 2nd Renewal Date- Ili_
Culture Tray Temp: -c Analyses): mer^R, sF Reviewed by:
Collection (Start) Date's-
Transferred by: Fed by.
DayO j/VP-
Day 1 ST
Day 2
Day 3Day 4
Day 5
Day 6
Terminated by:
9 ;
Eg
C| c
10
Email: [e-mail] Web Site: www.meritech-labs.com
ALLState:Zip:
Sample Site:So-
Sample Type: Q Grab # of containers:
Sampling Tima:Start Date:J - /JL Start Time:PM//■Jo
End Date: - 2.£> I 2—End Time:
TOXICITY TEST INFORMATION
IWC:%Test Concentrations (if multiple dilutions):
Comments:
Relinquished by: 2.Time:AM,PM
Time:AM ’M
Time:AM,PM
Date: Time: AM PM
Date' Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
!Date: Time: AM PM
Sample Temperature (°C):
SAMPLE RECEIVING (Laboratory Use Only)
Date: Time: PMAM
Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
3/^/nRecei'Date:PM
/
WHITE = Laboratory copy YELLOW = Client copy
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Test Organism: J53) Ceriodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
I | Mysldopsis bahia (shrimp)
Test Required: 0 Chronic (7 days)
Acute (24-48 hours)
%
/zip
/3IQ
16^°
Relinquished by:
Received by: MJyuOfMr.? ._______________
Sample Temperatures (°C): I. B / j.Q //
’y: zz/ f _________
Sample TemperatC>S / g'V/
PO#:
NPDES#: NC
Phone: /‘f)
Pipe #: /
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10.00 AM IF AT ALL POSSIBLE
*” SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AND COVERED WITH ICE “*
Collector's Name: Print: Signature:
SAMPLE INFORMATION
£3 Composite
CLIENT INFORMATION
~r~ &#'/'(?s-i
/ /&&C1 lS7l~~
Client:
Contact Person:
Address: f>o, fig/
City: _______
County: A « j a.
Method of Shipment: UPS Fed EX Meritech Pick-up
” Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery ”
Time: AM
■Sampl^Conditiom^£^^
Date: 3//.3
Date: 3/(3 /I'i-
Date: 3/43
MERITECH, INC.
B/oassay gamp/y Qfialn pf Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
AM PM
SHIPPING INFORMATION
JL/ZX
Meritech Sample ID #: 031364
EQUAL VARIANCE t TEST
18.3 18.9
t = = -0.785
0.849
Degrees of freedom = 22
Critical t = 2.508
-0.785 < 2.508
Chronic Test PASSES
n°LT siHnificant difference in reproduction between the Control and the effluent evaluated at a 99% confidence interval.
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date:3/27/2012
Facility. Clayton NPDES # NCOO 25453 Pipe#: 001 County: Johnston
Comments I
x
Signature of Laboratory Supervisor
MAIL ORIGINAL TO:
Test Initiation DatefTime 3/13/2012 4:35 PM Avg Wt/Surv. Control[J0.687 Test Organisms
% Survival 97.5
Avg Wt (mg)0 670 Hatch Date:3/12/12
% Survival 100.0 Hatch Time: 3:00 pm CT
Avg Wt (mg)0.748
L 1 % Survival[97 5
Avg Wt (mg)[]0.673
2 % Survival[]100.0
Avg Wt (mg)[ 0.649
[4 % Survival [100.0
Avg Wt (mg)0.739
8 % Survival 100 0
Avg Wt (mg)0.697
DWQ Form AT-5 (1/04)
1
3/12/2012
Dilution H2O Batch #
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt (°C)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
[ 0.5 | Surviving #
Original #
Wt/onginal (mg)
Surviving #
Original #
Wt/original (mg)
I Surviving #
Original #
Wt/original (mg)
I Surviving #
Original #
Wt/onginal (mg)
J Surviving #
Original #
Wt/original (mg)
r Cultured In-House
Outside Supplier
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Normal
Hom. Var.
NOEC
LOEC
ChV
Method
Environmental Sciences Branch
Division of Water Quality
NCDENR
1621 Mail Service Center
Raleigh, NC 27699-1621
614
46
55
215
10
10
0.716
10
10
0.732
1
9
10
0.616
615
48
55
217
616
48
56
218
______1
8,22 / 7 86~
7.49 / 6.49
25.7 / 25.0
2
3/14/2012
10
10
0.704
10
10
0.697
10
10
0617
10
10
0.666
2
10
10
0.716
617
44
54
214
2
8.22 / 8.01
7.39 / 6.81
25.5 / 25.5
7.40 / 6.90
25.5 / 25.5
10
10
0.625
9
10
0 752
10
10
0.716
10
10
0.728
10
10
0.762
10
10
0.629
10
10
0.786
10
10
0.732
Survival
Critical
10
10
10
10
10
Calculated
20
18
20I
20
20
Critical
241
2.41
2.41
2.41
2.41
0
8.22 / 8 21
7.52 I 7.19
25.7 / 25.5
0
8.24 I 8.13
7.48 / 7.28
25.7 / 25.5
10
10
0.661
10
10
0.676
10
10
0.590
1______________2
8.23 / 7.81 8.23 / 7.93
7 47 / 6.56
25 7 i 25.0
24 5
60
241
1206
<0.1
0.5
10
10
0.758
3
3/15/2012
24.0
68
261
1257
<0.1
1.2
10
10
0.640
10
10
0.836
3
10
10
0.645
4
10
10
0.702
Stats
Cone.
0.5
1
2
4
8
5
8.27 / 7.86
7.54 / 6.90
25.1 / 24.8
6
8.14 / 7.93
7.66 / 6.90
25.3 / 25.0
6
8~09 / 7.74
7.59 / 6.45
25.3 / 25.0
Overall Result
ChV | >8
22.5
58
250
1196
<0 1
1.8
4
8 30 / 8.15
7.66 / 7.39
25.0 / 24.4
Labprtrtorw Meritech, Inc._____________
——
SjgnatuXof Operator in Responsible Charge
% Eff. Repl.
[Control | Surviving #
Original #
Wt/original (mg)
Growth
Calculated
-2.0270
-0.0777
0.5375
-1.7938
-0.7059
Survival
r&
n?
8
>8
>8
Steel's
Growth
Fl;
Fli
8
>8
>8
Dunnet’s
3
8.20 / 8.19
7.54 / 7.32
25.4 / 24.8
4
8.25 / 8 22
7,60 / 7 30
25.0 / 24.4
5
8?26 / 7.93
7 43 / 6.90
25.1 / 24.8
Day
3 ~
8.23 / 8 09
7.56 I 7,35
25.4 / 24.8
•••••••••••••••••••••••••••••••••••••••••••a
ClaytonClient:# of Organisms per Chamber: 10 3/13/12Start Date:Time:
npdes #: NC0025453 Test Vessel Size: 400 ml 3/20/12End Date:Time:
Date/Time Fed: 3/13/12 Randomization:Yes No
Date/Time Born: 3/12/12 I# of Reps: 43:00 PM CT Incubator #:
Transferred by:Fed by:
Date Time Initials Date Time 1 Time 2 Initials Time 3 Initial
3/14 3/13Day 1 Day 0
3/15 3/14Day 2 Day 1
3/16Day 3 Day 2
3/17Day 4 Day 3
3/18 •k3/17Day 5 Day 4
3/19 ■k kDay 6 Day 5
Day 6
Initials/Signature:
End Date:'
End Time:
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Initials
IK
Initiated by:
Test Termination DatafyY* -
3/20/12
3/18 ,
3/19
*■
= Weekend, only 2 feedings needed
935^
3/15 jd^. VA- m2-
3/16 I'VV-v /17
r"foA\ ST'
Test Solution Volume: 250 ml
Temp, of Stock:
Chemical and Physical Determinations
RemarksConcentration: control 5-64-52-3
Batch # :
InitialpH:
D.O.:
Temp.:Initial
Final
Conductivity:Initial
Final
Residual Chlorine:
RemarksConcentration:0.5%
InitialpH:
Final
D.O.:Initial
Final
Temp.:Initial
Conductivity':
RemarksConcentration:1.0%
InitialpH:
Final
D.O.:
InitialTemp.:
Final
InitialConductivity:
Final
Residual Chlorine:
Client: Clayton NPDES#:
Test Organism: Pimephales promelas
Final
Initial
Final
Residual Chlorine:
Hardness:
Alkalinity:
Initial
Final
Final
Initial
Final
^-27
NC0025453 Start Date; 3/13/12
Analyst(s): mer, MR. ST End Date: 3/20/12
2-3
'7'^?
7 .§6
j
am
Z<9./
713
7.97
ZiT"
0-1
Vo
IM
7.^3
-2S 3
^3/ a.31
<0./
Day
3-4
bib -
■ i
4-5
8, \7>
1-2
6/r-
r,
7-/7
237
./
vr ■■
.74 -
2-3
^22-
74^
6.7/
'Z-Zie
xo.
6-7
_£ZC_
7 71
7.5?
P5~,Z>
33c am
6-7(cn7^
nr
<213 cm
sz
Day
3-4
<Z23
7TS ,
•333
251
4 p. I
0-1
(37
S.ZH'
g.l3
7 Vi'
rr.7
399
ip./
1-2
%.1-V
770
25
OT2_
^3
1-2
.22?
zoJ
3m___Zm I A31
Z£>- /
~W
Day
3-4
S./^?w:
24 .f
I
2.3.7
av-
zo. I
4-5
3.14
7 C?
334
i
MER!TECHr INC.
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls, Inc.
5-6
21 aU-
7^6
7 53
7 r/
'S35T
xi>.7
5-6
8.9Lo
7.^
it irJ
2M- f
TQq
ZOJ
o-l
7./I
2T-7
6-7
y./<b
W
?.
7 6^ H.55
7.75-1 W.
W
Tii mr
I
Time: ^3^
Time:
Chemical and Physical Determinations
Client:Time:
3/20/12 Time:
Concentration:Remarks
pH:
D.O.:
Temp.:
Conductivity:
Concentration:Remarks
pH:
D.O.:
Temp.:
Conductivity:
Remarks0-1
D.O.:
Temp.:
Conductivity:
Concentration:6-7 Remarks2-30-1
z/ve.
ENVIRONMENTAL L-/ABO RATOR I 6 S
of \A'^tirr Ttchno/^oy forf Coor^o»f. toe
Clayton
Test Organism: Pimephales promelas
Concentration:
PH:
3/h
100%
PH:
Conductivity:
Residual Chlorine:
_______Hardness:
Alkalinity:
4.0%
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
2.0%
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
~7. 3H
Tyr
<0.|
Day
3-4
8.0%
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine:
0-1
2£A.
■7.17
Day
3-4
7^
....
4-5
3^
^o.l
Day
3-4
~7TT
f£.
2-4 X-
.7 % 4
xo.l
2-3
^22-7 a 1 7^
c?i?
zTa r
2-H7
^c.\
Z7335
2-3
^22-
~7>
7.^
77/.
Z££_
7-AO
_z£7C
2^7
6-7
fr./r
Z1I
25^
A15
6-7
T7y~
3
7/i5 W
XT. 3
3>.S7iC)
Ao7?
4-5
9,3^ VW
7- c-* A
-T Sj
zJ-rO
zy.y
yo./
5-6
77)
7 >7
237,.VW
^.1
0-1
~),2£>
^.7
VFW
AO. I
5-6
^,95
32». g'
w
V7
4-5
g-,23-19,
7. C‘^>
’^5(o
ZLP/ 1
NPDES#: NC0025453 Start Date: 3/13/12
Analyst(s): mer, MR, ST End Date:
6-7wv
TtlR 7W
9. 7 g
077
v-Oz I
1-2
3.^
2577
Q5i
7^2^
<6?- /
1-2
y.zz-l^wz-'
ToT~
Day
3-4
320
W
7 33
'Vo
ZCM
I 1-2
7W
AO-/
"2.5-0
2-3
Y.22-
Wr
W~
zTT
wr
^(z' I
Page 2 of2
//:CO
1-2
?.?7
tFT
WT w
7S~.p
7Jo>
<o. I
5-6
743
7.^^ 7. A3
VW
. 7*/^
^93
1
4-5 5-6
r. 33
^o<\
^6/
Page: 1 of 2Facility. Clayton
Outfall: NPDES #: NC0Q25453
Organism: Pimephales promelas
SURV %# ORG.CONC REP
AoAO),(.o\lo(o, lloA
O 335.94 ICQ0.4 UpIO4. lloB
O.(o46 100IOc
io oaoa 10033 I ‘ Ob 4.00D
0/3403.34 100833. CO IDA
1000.3533.4533 833.55 3.521 10B0.5%
too933.23 830.99 0.3! Io3. IQ 10c
IOC0134to9?>io.54 239.30 q.%o IOD
Q.Ffio843.30 239.80 5.30 10 IGOA
0,loV383490 233.53 10 100(o, 13B1.0%
A go0.333§19.33 330. So 3.50c
IO 0 330 10033134D
PAN WT.
(mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF ORG.
(mg)
MEAN
WT./ORG. (mg)
c o
O
PAN + ORG.
WT. (mg)
MERSTECH, INC.
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls. Inc
3J
Date of Test: 3/13/12
Initial weights taken on: 3-1094 by; Dr
Final weights taken on: 3>'''o£^'~lQ by: S~f
ClaytonFacility:
NPDES#: NC0025453
Organism: Pimephales promelas
SURV %# ORG.CONG REP
OJojLb10A
10 \ooCdJLhO0. icicleB2%
ms3 W.08 (g.05 10q10c
B3O.I3 O.(o9A(p.SR 10010D
ms-i ML 45 to, lol 10 10OG. (olo \A
O 034SBlo.RM- 3444 \I0Q\0B4%
34.4H 0. %3(c.1003, 30 108IG.O3c
WOo 3Kq.9S 04 GO4.GO 10QIOD
31333^Dlo ,00 104, Ko 0.114 ICC)A
4.O-L iOo395,l(o 10soa .50 0.304B8%
315.04 85K-43 (.0,40 0.04010 \0P)c
.00344 0.393Mja 10010D
PAN WT.
(mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF ORG.
(mg)
MEAN
WT./ORG. (mg)
Page: 2 of 2
Outfall:
MERITECH, IIVC.
ENVIRONMENTAL LABORATORIES
x* Di^isityn of FecAino/o^y anef Controls. Inc.
Date of Test: 2/21/12
Initial weights taken on: i(cr |Q> by: S~F
Final weights taken on: 3 by: ST
£50,^
8^44
PAN + ORG.
WT. (mg)
Start Date:Client: Clayton NPDES#: NC0025453 2/21/12 Time:
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST 2/28/12End Date:
ControlConcentration
Day 0 Day 1 Day 2 Day 3 Day 6
oRep#1
0 0oRep #2
0 0
Rep #4 O
0.5%Concentration
Day 6Day 1 Day 2
Rep #1
0 d0o
0 000
00o00 0
1.0%Concentration
Day 6 Day 7Day 2 Day 4Day 0
Rep #1
60Rep #2
0Rep #3
Rep #4 c)
0
0
o
MERITECH, INC.
Mortality Data: Chronic Fathead Test
&
Day 7
0
Day 0
0
Day 3
o
Day 4
0
0
Day 5
0
Day 7
0
Day 4
I
Day 1
C)
Day 5
C)0
0
0
0
Rep #3 T)
Rep #4 0 0
Rep #2 0
Day 3 Day 4 Day 5
£2__b___Cl
0 0
0 0
Time:
Reo#3 C Q
2/21/12 Time:Client: Clayton NPDES#: NC0025453 Start Date:
Time:2/28/12Test Organism: Pimephales promelas Analyst(s): mer, MR, ST End Date:
2%Concentration
Day 3Day 2
Rep #1
00 0 &
Rep #2
t>0Rep #3
0 0Rep #4
4%Concentration
Day 7Day 4 Day 5Day 1 Day 2 Day 3
0
0Rep #3
jRep #4
8%Concentration
Day 6 Day 7Day 5Day 2 Day 3 Day 4
Rep#1
C)0
0 00Rep #3
0
0
0
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Day 7Day 0
0
Day 4
0
Day 6
o
Day 0
Day 1
0
Day 6
0
Day 5
0
o
0
Day 0
Rep#1
Rep #2 O
Q_
o • 0
Rep #2
Day 1
o o
Rep #4 (J
03)384Meritech Sample ID #:
Fax: [phone]
Email: [e-mail] Web Site: www.meritech-labs.com
Client:
State: Zip:
SAMPLE INFORMATION
# of containers:
J - /^LSampling Time:Start Date:Start Time.AM PM//.^g
End Date: - / 5 - 2^/MOEnd Time:AM PM
TOXICITY TEST INFORMATION
IWC:%Test Concentrations (if multiple dilutions):
Comments:
Relinquished by: Time: AMDate:
AMTime: Date:
Time:AMDate:
Time: AM PMReceived by: Date:
PMTime: AMRelinquished by: Date:
PMTime: AMReceived by: Date:
PMTime: AMRelinquished by: Date:
Time: AM PM• Received by: Date:
Sample Temperature (°C):
SAMPLE RECEIVING (Laboratory Use Only)
3-/3-/Z PM,Time:AMDate:
I Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
Test Required: M Chronic (7 days)
lJ Acute (24-48 hours)
Sample Site:
Sample Type: EJ Grab
Test Organism: Ceriodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
| | Mysidopsis bahia (shrimp)
■* CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
Received by: —-
Relinquished by:
£3 Composite
/62O
/6^G
MERITECH, INC.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
2.
3/13
Method of Shipment: UPS EJ Fed EX O Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery
PO#:
- "'NPDES#: NC O <5 25/5’3
Phone: I cl)
Pipe#: 00/
Relinquished by:
Received by: hQ
Sample Temperatures (°C): I - B
CLIENT INFORMATION
(/[
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AND COVERED WITH ICE m
Collector's Name: Print Signature: Xtf
SHIPPING INFORMATION
/ /.3 /
Contact Person:
Address: 1^0/
City: y/. (/pt'i______
County:
PM
•M
PM
Meritech Sample ID#'
Email: [e-mail] '
CLIENT INFORMATION
State:
Sample Site:
# of containers: Sample Type:
Start Time Sampling Time: Start Date:
End Time:End Date-
IWC: 3 . Test Concentrations (if multiple dilutions):%
Comments:
AMTime:Date:
AMTime.Date:
AMTime:Date:
PMAMTime: Date
PMAMTime: Date
PMAMTime. Date:
PMAMTime: Date:
PMAMTime: Date:
Sample Temperature (°C):
Relinquished by:
PMTime- Date:Receiy
/
YELLOW = Client copyWHITE = Laboratory copy
Method of Shipment: O UPS
** Samples shipped on
- CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
Test Required: 1^3 Chronic (7 days)
r~l Acute (24-48 hours)
PM_
&
3' ! 2-.
3 ’f
MERITECH, INC.
B/oassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone. [phone]
TOXICITY TEST INFORMATION
Test Organism: Ceriodaphnia dubla (water flea)
Pimephales promelas (fathead minnow)
0 Mysidopsis bahia (shrimp)
/ 2. e>
///'^
J, J4-
Grab
7
PCfc.____________________
NPDES#: NC 3
Phone: h
Pipe #: 7)0 /
Zip:
>y: /// jr /
Sample Temperatyf^<C): xSl1
Fax: [phone]
Web Site: www.meritech-labs.com
SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED AND COVERED WITHJCE”
Signature:
AM PM
/(l.’OO AM PM
Client: <? f
Contact Person: (JixTrte-iA, /zg/>Z
Address: 7?^. 5*0X //oGO "j)_
City: /g/7
County. A/?
SHIPPING INFORMATION
‘p
3/ 2- Time- AM
Sample Condition: V
Fed EX Meritech Pick-up
Friday must be FedEx and must be clearly labeled for Saturday delivery 'r
SAMPLE RECEIVING (Laboratory Use Only]
7.
• Relinquished by: <3.^,
jff
Received by: y
Relinquished by:
Received by:
Relinquished by:
Received by:
• Relinquished by:
Received by:_
Collector's Name: Print:
SAMPLE INFORMATION
® Composite
Fax: [phone]
Email: [e-mail] Web Site www.meritech-labs.com
CLIENT INFORMATION
State: Zip:
Sample Site:
Sample Type:
Sampling Time: Start Date:Start Time:PMkAI
End Date:AM PM
Signature.
TOXICITY TEST INFORMATION
Test Organism:
IWC: c2 %Test Concentrations (if multiple dilutions):
Comments:
Date: Time AM
Date: Time. AM
Relinquished by:Date:Time- AMcReceived by:i ime: Date. AM PM
Relinquished by: Date Time: AM PM
Date. Time: AM PM
Time: AM PMDate:
Received by: Date: Time AM PM
Sample Temperature (°C):
SAMPLE RECEIVING (Laboratory Use Only)
3-PMDate:Time:AM
Sample Temperatures (°C): / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
Received by:
Relinquished by:
Relinquished by:
Received by:
Test Required: Chronic (7 days)
Q Acute (24-48 hours)
0 Cerlodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
Q Mysidopsis bahia (shrimp)
J-/ ST-^.012-
3 '2^0! -2-
/O.'OO
End Time: /^}-00 (
V
PO#:
NPDES#: NC 0 02 S'‘/S'3
Phone: fa / -/
Pipe#. [
>7
SAMPLE INFORMATION
0 Grab S. Composite
' :2D
3^.
Relinquished by:
Received by:
/ /-Zz I
MERITECH, INC.
Bioassay Sample Chain Qf Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
SHIPPING INFORMATION
“* SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AND COVERED WITH ICE ***
Collector's Name: Print: / Signature:
Method of Shipment: UPS 0 FedEX Q Meritech Pick-up
” Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery
Meritech Sample ID #:
c,ient; / f
Contact Person: ^3-.^ /2-e‘(k
Address: 7^ <9. Box ^2^ //wo
City: C tn__________________
County: Tfo s
# of containers: 0
Ti k y
-----► CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
PM
Transform:NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
Data PASS normality test (alpha = 0.01). Continue analysis.
D =
W =
0.0537
0.9744
Title:
File:
Critical W
W
clayton
clayton
= 0.8840 (alpha = 0.01 , N = 24)
= 0.9160 (alpha = 0.05 , N = 24)
Transform:NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance ,
Calculated Bl statistic =(p-value = 0.3424)5.6439
Data PASS Bl homogeneity test at 0.01 level. Continue analysis.
Critical B = 15.0863
= 11.0705
5)
5)
Title:
File:
clayton
clayton
(alpha = 0.01, df =
(alpha = 0.05, df =
Transform:NO TRANSFORMATION
•ANOVA Table
SOURCE MS FDFSS
0.0064 2.1452Between50.0320
Within (Error)0.0030180.0537
Total 23 0.0856
(p-value = 0.1064)
Critical F
Ho: All equal (alpha = 0.05)Since Critical F FAIL TO REJECT
Title:
File:
= 4.2479
= 2.7729
(alpha = 0.01, df = 5,18)
(alpha = 0.05, df = 5,18)
clayton
clayton
F <
Transform:NO TRANSFORMATION
Dunnett's Test Ho:Control<TreatmentTABLE 1 OF -2
GROUP IDENTIFICATION T STAT
Dunnett critical value = 2.4100 (1 Tailed, alpha = 0.05,df = 5,18)
Transform:NO TRANSFORMATION
Dunnett's Test Ho:Control<TreatmentTABLE 2 OF 2
GROUP IDENTIFICATION
Title:
File:
Title:
File:
1
2
3
4
5
6
1
2
3
4
5
6
NUM OF
REPS
TRANSFORMED
MEAN
4
4
4
4
4
4
0.6698
0.7480
0.6728
0.6490
0.7390
0.6970
MIN SIG DIFF
(IN ORIG. UNITS)
0.0930
0.0930
0.0930
0.0930
0.0930
MEAN CALCULATED IN
ORIGINAL UNITS
0.6698
0.7480
0.6728
0.6490
0.7390
0.6970
% OF
CONTROL
13.9
13.9
13.9
13.9
13.9
DIFFERENCE
FROM CONTROL
-2.0270
-0.0777
0.5375
-1.7938
-0.7059
-0.0783
-0.0030
0.0207
-0.0692
-0.0273
SIG
0.05
control
. 5
1
2
4
8
control
. 5
1
2
4
8
clayton
clayton
clayton
clayton
Fisher's Exact Test
= == = =
NUMBER OF
IDENTIFICATION DEAD ALIVE TOTAL ANIMALS
CONTROL 40139
. 5 0 40 40
80TOTAL791
b value is 0.(alpha=0.05) is negative.
Fisher's Exact Test
NUMBER OF
TOTAL ANIMALSIDENTIFICATIONALIVEDEAD
40391CONTROL
403911
802TOTAL78
Fisher's Exact Test
NUMBER OF
TOTAL ANIMALSALIVEIDENTIFICATIONDEAD
40139CONTROL
404020
Critical Fisher's value (40,40,1)
no significant difference
Critical Fisher's value (40,40,39) (alpha=0.05) is 33.0. b value is 39.
Since b is greater than 33.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
8079TOTAL1
b value is 0.
Fisher's Exact Test
NUMBER OF
TOTAL ANIMALSIDENTIFICATIONALIVEDEAD
40CONTROL391
400404
8079TOTAL1
b value is 0.
Fisher's Exact Test
NUMBER OF
TOTAL ANIMALSALIVEIDENTIFICATIONDEAD
40CONTROL391
404080
8079TOTAL1
(alpha=0.05) is negative.b value is 0.
Summary of Fisher's Exact Tests
GROUP IDENTIFICATION
1
Critical Fisher's value (40,40,1)
no significant difference
Critical Fisher's value (40,40,1) (alpha=0.05) is negative,
no significant difference
CONTROL
.5
NUMBER
EXPOSED
40
40
NUMBER
DEAD
1
0
SIG
0.05
Critical Fisher's value (40,40,1) (alpha=0.05) is negative,
no significant difference
Transform:NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning
Title:
File:
D =
W =
clayton - survival
claytonsurv
0.0150
0.6138
Critical W
W
The first three homogeneity tests are sensitive to non-normality
and should not be performed with this data as is.
= 0.8840 (alpha = 0.01 , N = 24)
= 0.9160 (alpha = 0.05 , N = 24)
“SERVICE”“ENVIRONMENT"
January 3, 2012
Re: Toxicity Results
To Whom It May Concern:
Please call me directly at 919-553-1536 if you have any questions.
Si ■ely,
James Warren, ORC
Enclosed please find the Ceriodaphnia Pass/ Fail and Fathead Minnow toxicity results lor
the Little Creek Water Reclamation Facility.
TOWN OF CLAYTON
OPERATIONS CENTER
VEHICLE MAINTENANCE
[phone]
ELECTRIC SERVICE
[phone]
WATER RECLAMATION
[phone]
PUBLIC WORKS
[phone]
Environmental Science Branch
DWQ,NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
653 Highway 42 Wcsi ♦ P.O. Box 879 • Clayion, North Carolina 27520 • [phone] • Fax [phone]
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 12/15/11
Facility: CLAYTON WWTP
Comments: dilution water batch
918 and 919 used.
Work Order:
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 645 7 8 9 10 11 12
% Mortality Avg.Reprod.
# Young Produced 18 20 15 18 18 21 20 19 11 20 20 17
Adult (L)ive (D)ead L L L L L L L L L L L L
1 2 3 645 7 8 9 10 11 12
PASS FAIL
# Young Produced 17 20 15 21 18 18 20 21 18 20 17 20 X
Check One
Adult (L)ive (D)ead L L L L L L L L L L L L
1st sample 1st sample
PH
Control 8.18 8.01 8.18 8.03 8.06
Sample 2: 12/07/11
Treatment 2 8.19 8.05 8.15 8.06 8.16 8.06
1st
Grab DurationComp.
Sample 1 24 hrsX
Sample 2 24.8 hrsX
Hardness(mg/1)44
7.70 7.50 7.73 7.62 7.72 7.64
Spec. Cond.(pmhos)169 1280 1270
Treatment 2 7.72 7.75 7.49 7.65 7.60
Chlorine(mg/1)<0.1 <0.1
LC50/Acute Toxicity Test Sample temp, at receipt(°C)1.4 1.1
(Mortality expressed as %, combining replicates)
%%%%%%%%%%Concentration
%%%%%%%%%%
start/end
Control
PH D.O.
Organism Tested:Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT-1 (3/87)
7.39 ____
Method of Determination
Moving Average
Spearman Karber
Probit
Other
Chronic Test Results
Calculated t =
Tabular t =
% Reduction = -3.69
High
Cone.
I
Note: Please
Complete This
Section Also
D
I
L
U
T
18.75
Treatment 2
S
A
M
P
S
A
M
P
0.00
Treatment 2
Control CV
15.365%
0.00
Control
18.08
Control
2nd
P/F
LC5 0 = %
95% Confidence Limits
% - - . %
e
n
d
e
n
d
e
n
d
% control orgs
producing 3rd
brood
83.3%
Mortality
start/end
2nd sample
RT7
D.O.
Control
Effluent %: 2.0%
TREATMENT 2 ORGANISMS
s
t
a
r
t
1st sample
s
t
a
r
t
2nd sample
s
t
a
r
t
1st sample
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
Complete This For Either Test
Test Start Date: 12/07/11
Collection (Start) Date
Sample 1: 12/05/11 ;
Sample Type/Duration
X _______
Signature /f^erator^i Responsible Charg
x_ __ __L; , <2 zZZ-
Signature of Laboratory Supervisbr“X:
NPDES#: NC0025453 Pipe#: 001 County: JOHNSTON
Laboratory Per£orming Test: MERITECH LABS, INC. --------------------------------
* PASSED: -3.69% Reduction *
rev. 11/95 (DUBIA ver. 4.41)
Pipe #: 00/
'a/2L//'
Sample 2. ^/? // /Sample 1:
2
12
. Batch #
L~
Total Produced
io-
I.)r:'
PH5 2nd Sample
Control ^4)
Sample
D.O.
/.(cL.Control
Sample
LI
Temp.
Total Produced ^0 Control
Sample
final
Sample
1
r
L_
O
3
o
L
4
0
u
2
L.
2
Sample
2
Fed by:
3. A.te
3
Percent of Control producing third brood: ?k?t3>/0
Test Sample Organism Reproduction
_____________x_____
I Effluent %: Q |
Day #2
# Young Produced
Adults Live / Dead
_____Day #5
# Young Produced
Adults Live / Dead
_____Day #7
# Young Produced
Adults Live / Dead
3
O
Sample 1
Sample 2
Duration
hours
hours
6
O
L
6
O
L
12
O
L Sample Information
100% pH
g,3Z
2V
1
O
L
8
O
L
o Vi n
__ft/-Z7ArO
•10^-
__hours
.°C
1?4
4
£
L
7
o
L
L
2
^2
0
d
2nd Sample
7,7?- 7.^
8
O
L
9
C>
Transfer Day
Hardness
WL)
Spec. Cond.
(umtios/cm)
Chlorine
(mg?L)
Receipt Sample
Temp (°C)
1980
t
5
o
L
12
O_
7
o
10
O
L
11
Q
L
9
o
L
G/C?
C
c
<0A
11
o
L
9 10w
I
11
t;
L_
b / 8 9 10 1|
±2Q[ ?A|/o|/o| 7 IqlW
|lTz_ Il RITlI l 5
1st Sample
7.7>
7.7S
initial linal
1
1/ .m2/ 3 4Z._5z 6____7,MW
——L G. [_
J 2 3 4 5 6 7
^65’ 7.bO
■mtial final
L
lTT l lull
1st Sample
8.1'I
initial fuwi initial final
2nd Sample
initial final
1st Sample
770
7.77x 7.^
initial Anal
Client: C-\a.o'VotA______
NPDES #■. NC OOZ.-6 53
Dilution Water: Lake Reidsville
Test Organism Source: Tray # Sp
Stirred / Aerated for P.O.: Y /(N) *
1st Sample
^'/7X
initial final
10
o
L
- 5 A . -T'6- . 7.. 8y, 9 10
WW7-7|3/7|<mW
l r- i w | l rc| c I tr
. 4 5 6^ 7 8 9 10
Incubator #:
Date End:
Time End:
— Time:
Time:
Reviewed by:
Collection (Start) Dates:
Transferred by:
DayO jW?
Day 1
Day 2 ^L'
Day 3
Day 4
DaysyiyL
Day 6
Terminated by:
1st Sample
gjgigij
Sii’7 ^.cQ)
initial final
1st Sample
15'^
initial
8 9 10 11 12
X-.
12
LL u
l.
Id L
4-, £ 3Tfwn
L
Comments:
Moritech, Inc.
Mini Chronic Pass/Fail Test: Ceriodaphnia dubia
County. 7\Vi n fa Date Start’
Date / Time of Culture Transfer: l^-b-ll / ft»3-7ArA Time Start
Date / Time Neonates born: /2 :|O^-1st Renewal Date
_ Age of Neonates at Test Start: J^,l7 hours 2nd Renewal Date
---------------- - Randomized^/N Culture Tray Temo: Z5'.1/ °C u..' -___•
Control Organism Reproduction ------- - Ana,yst(s)- S T
Day #2_______ 1 2
# Young Produced O
Adults Live / Dead L
_______Day #5_______1
# Young Produced S/fe
Adults Live / Dead
________Day #7
# Young Produced
Adults Live / Dead
Merited) Sample ID #;
State:Zip:
SAMPLE INFORMATION
Sample Site:
it of containers:
Start Time.^L PM
End Time: AM PM
Signature:
IWC:%Test Concentrations (if multiple dilutions):
Comments:
Date:Time:
Date:Time:
Date; Time:
Date: Time- AM . PM
Date: Time: AM PM
Date- Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Sample Temperature (°C):
3:<oDate: Time:AM
Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Test Required: Chronic (7 days)
■O Acute (24-48 hours)
Client:____
Contact Person:
Address:
City:
County:
Test Organism: Ceriodaphnia dubia (water flea)
Pimephales promeias (fathead minnow)
Mysidopsls bahia (shrimp)
//•/^
PM
Method of Shipment: UPS
** Samples shipped on
MERITECH, INC.
B/oassay Sample Chain of Custody
542 Tamco Rd, Reidsville NC 27320
Phone: [phone]
Email: [e-mail]
Sample Type; Grab
PO#:
NPDES#: NC 00^5-4/5-3
phone:
Pipe#: /•
p P t
M Composite
Sampling Time: Start Date: / JL “ *-2 O / /
End Date: / 2. ' 6> "2. o I (
CLIENT INFORMATION
/0^k -f _________ __ __________
S Irish' s'
''Tok st 6
Relinquished by:
Received by: CJL-
Sample Temperatures (°C): 1-^ / I. / /
Fax: [phone]
Web Site: www.meritech-labs.com
SHIPPING INFORMATION
42/ ^,2. ari
- f!
O Fed EX 0 Meritech Pick-up
Friday must be FedEx and must be clearly labeled for Saturday delivery **
SAMPLE RECEIVING (Laboratory Use Only)
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED AND COVERED WITH ICE *** ■
Collector’s Name: Print: /.
TOXICITY TEST INFORMATION
ft!SO .AM PM
3 [ )& AM
I2.0&45Meritech Sample ID #:
Fax: [phone]
Email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
Address:
State:
SAMPLE INFORMATION
Sample Site:
Sample Type: O Grab S’* Composite # of containers:
Sampling Time: Start Date: Start Time: AM PM
isyfcZEnd Date://AM.PM
Signature:
TOXICITY TEST INFORMATION
Test Organism:
iWC: %Test Concentrations (if multiple dilutions):
Comments:
Time: PM
Time:PM
Relinquished by;
Time: AM
Time: AM , PM
Time: PMAM
Date: Time. AM PM
Date: Time: AM PM
■Received by: Date: Time: AM PM'
Sample Temperature (°C):
SAMPLE RECEIVING (Laboratory Use Only)
Date: Time: AM
/ / Sample Condition:
WHITE = Laboratory copy YELLOW “ Client copy
Relinquished by?
Received byr'
Date:
Date:
fX Ceriodaphnia dubia (water flea)
Pimephales promelas (fathead minnow)
Mysidopsis bahia (shrimp)
Received by:
Relinquished by:
Received by:
Relinquished by;
Test Required: Chronic (7 days)
Q Acute (24-48 hours)
City:
County:
//37
54 O ki
MERITECH, INC.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]'
Method of Shipment UPS Fed EX
“ Samples shipped on Friday must be FedEx and m
-------------,------L
/ /i \
i I Meritech Pick-up
ust be clearly labeled for Saturday delivery **
Clien’: <z> f ______________
Contact Person:
Relinquished by: M-
Received by: M-lCLuXLAGy
Sample Temperatures (°C); I ■ j / /, I
End Time: -* CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED AND COVERED WITH ICE
Collector's Name: Print:iZfy, Signature:
SHIPPING INFORMATION
____ Date: /Z/y>r
Date: g /(
Date:
PO#:
NPDES#: NC
Phone; (cj / )
Pipe #: f
ZiP _____
'M
Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 12/15/11
Facility: CLAYTON WWTP NPDES#: NC0025453 Pipe#: 001 County:JOHNSTON
Laboratory Performing Test: MERITECH LABS,INC.
Reduction: -3.69%2.0% EffluentCONTROL
# Replicates 12 12
Female Live 12 12
Adult Male 0 0
Adult Dead 0 0
Adult Mortality 0.00%0.00%
# Neonates 217 225
Mean # Neonates 18.083 18.750
Standard Deviation 2.778 1.865
Coefficient of Variation 15.365%
Fisher's Exact Test
A = 12 b = 12
a/A = 1.00 b/B = 1.00
Success is:survival
Critical b value = 8
12 > 8
Test Passes!
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA
ORDERED OBSERVATIONS
i Group Neonates Centered i Group CenteredNeonates
1
2
3
4
5
6
7
8
9
10
11
12
C
E
C
E
E
C
E
E
E
C
C
C
11
15
15
17
17
17
18
18
18
18
18
18
-7.0833
-3.7500
-3.0833
-1.7500
-1.7500
-1.0833
-0.7500
-0.7500
-0.7500
-0.0833
-0.0833
-0.0833
13
14
15
16
17
18
19
20
21
22
23
24
C
E
E
E
E
C
C
C
c
E
E
C
19
20
20
20
20
20
20
20
20
21
21
21
0.9167
1.2500
1.2500
1.2500
1.2500
1,9167
1.9167
1.9167
1.9167
2.2500
2.2500
2.9167
a - 12
The test concludes that the proportion of survival is not
significantly different for the control and the effluent groups.
B = 12
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.)
COEFFICIENTS AND DIFFERENCES
i x(n-i-1)x(i)x(n-i-l) - x(i)a(i)
1w X 108.7051
123.1667
Calculated W = 0.883 Critical W = 0.884
0.883 < 0.884
Test Failed!
Wilcoxon Rank Sum Test
Rank Sum - Effluent Group(treatment):Control Group: 143157
Mann-Whitney U = 79.0 nl(treatment) = 12 n2(control) = 12
The critical value from TABLE A.15 (0.01 Level of T)for the above n values:
Critical T = 109
157 > 109
TEST PASSES
Chronic Test PASSES
The reduction was less then 20%
-7.0833
-3.7500
-3.0833
-1.7500
-1.7500
-1.0833
-0.7500
-0.7500
-0.7500
-0.0833
-0.0833
-0.0833
0.4493
0.3098
0.2554
0.2145
0.1807
0.1512
0.1245
0.0997
0.0764
0.0539
0.0321
0.0107
10.0000
6.0000
5.3333
3.6667
3.6667
3.0000
2.6667
2.0000
2.0000
1.3333
1.3333
1.0000
1
2
3
4
5
6
7
8
9
10
11
12
2.9167
2.2500
2.2500
1.9167
1.9167
1.9167
1.9167
1.2500
1.2500
1.2500
1.2500
0.9167
The reproduction data is not normally distributed
evaluated at a 99% confidence interval
The Sum of the Ranks of the effluent group is greater than the significant
value from the table. Therefore there is no significant difference between
the means of the effluent and control groups and the test passes.
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date: 12/19/2011
Facility. Clayton NPDES # NC00 25453 Pipe #: 001 County: Johnston
Comments I
Signature of Laboratory Supervisor
MAIL ORIGINAL TO:
Test Initiation Date/Time 12/6/2011 3 45 PM Avg Wt/Surv. Control 0.766
% Survival 100.0
Avg Wt (mg)0.766 Hatch Date 12/5/11
% Survival 100.0 Hatch Time: 3:00 pm CT
Avg Wt (mg)0.696J
1 % Survival 100.0
Avg Wt (mg)0.693
[2
% Survival 97.5
Avg Wt (mg)0.686
4
% Survival 100.0
Avg Wt (mg)0.753
L 8
% Survival 100.0
Avg Wt (mg)0.688
DWO Farm AT.K. /■r/nxi
4
8.35 / 7.82
7.73 / 6,98
25.4 / 25.0
| Surviving #
Original #
Wt/original (mg)
Test Organisms
r Cultured In-House
Outside Supplier
Dilution H2O Batch #
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt (“Q
Water Quality Data
Control
pH (SU) Init/FIn
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
0.5 I Surviving #
Original #
Wt/original (mg)
J Surviving #
Original #
Wt/original (mg)
2] Surviving #
Original #
Wt/original (mg)
J Surviving #
Original #
Wt/original (mg)
Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Normal
Hom. Var.
NOEC
LOEC
ChV
Method
1
12/5/2011
589
44
57
219
0
8.18 / 7 96
7 56 / 6 84
25.3 / 24.7
10
10
0.678
10
10
0.653
10
10
0 726
10
10
0.740
10
10
0.658
1
10
10
0.780
1
8 23 I 7,74
7.50 / 6,47
25.7 / 24.6
2
12/7/2011
10
10
0.612
10
10
0.765
10
10
0.649
10
10
0.670
10
10
0.612
2
10
10
0.651
2
8 19 / 7.87
7 79 / 6.77
24 5 / 24.5
3
12/8/2011
10
10
0.738
10
10
0.801
10
10
0.656
10
10
0.706
3
10
10
0.801
10
10
0 723
10
10
0.794
10
10
0.727
10
10
0.707
10
10
0.807
5
8.25 / 7 72
7.70 / 6.93
25.4 / 24.8
6
8.21 / 7.89
7.72 / 6.77
25.0 / 24.6
0
8.25 / 7,97
7 50 / 7.20
25.3 / 24.7
590
42
54
222
591
42
52
232
1
8.25 / 7 80~
7.50 / 5.97
25.7 / 24.6
23.8
72
247
1265
<0.1
1.1
2
8.19 / 7,79
7.66 i 6.90
24 5 / 24.5
9
10
0.640
4
10
10
0.831
Stats
Cone.
0.5
1
2
4
8
Survival
Critical
10
10
10
10
10
Calculated
18
18
16
18
18
6
8.23 / 7.80
7 69 / 6.62
25.0 / 24.6
Critical
2.41
2.41
2.41
2.41
2.41
24.0
68
242
1298
<0.1
1.4
23.0
64
258
1290
<0.1
1.4
Growth
Calculated
1.5358
1 5906
1.7607
02797
1.7113
a-z° Eff. Repl.
[Control | Surviving #
Original #
Wt/original (mg)
Survival
ntn
8
>8
>8
Steel's
Growth
Fg
Fll
8
>8
>8
Dunnet's
Laboratory: Meritech, Inc.
---- -
^igpafure of Operator in Responsible Cha^ge^ ~ x-7 Z”)
3
8.18 / 832
7.73 / 7 67
24 3 / 24 8
4
8.30 / 7.69
7.77 / 6.86
24 8 / 25,0
5
8.20 / 7,87
7,71 / 6.58
25.4 / 24.8
Overall Result
ChV | >8
Day
3
8.15 / 8.26
7.70 / 7.60
24 3 / 24.8
•••••••••••••••••••••••••••••••••••••••••••a
ClaytonClient:# of Organisms per Chamber: 10 Start Date:12/6/11
0025453NPDES #: NC i est Vessel Size: 400 ml End Date:12/13/11
Randomization:Yes
Date/Time Born: 12/5/11 # of Reps: 43:00 PM CT
Incubator #:
Transferred by:Fed by:
Date Time Initials Date Time 1 Time 2 Initials
12/7 12/6Day 1 Day 0
12/8Day 2
12/9Day 3 Day 2
12/10Day 4 12/9Day 3
12/10Day 5 Day 4
Day 6 Day 5 *
12/12Day 6
Initials/Signature:
End Date:
End Time:
'xr
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
12/11
12/12
Initiated by: p-
Date/Time Fed: 12/6/11
tMcw-
*' *
No
L
Time 3 Initial
Test Solution Volume: 250 ml
Temp, of Stock:
Test Termination Data:
12/13/11
*
= Weekend, only 2 feedings needed
11:20/^
Initials
Day1 12/7 (0^0^
12/8 Cq [(V'- i/IaM--
12/n 7M
Time:
Time:
1-2 2-3 6-7 Remarks
PH
D.O.
Temp.
Conductivity
Remarks
D.O.
Temp.
Conductivity
Remarks
D.O.
Temp.
Conductivity
Time:
Time:
MERITECH, IIMC.
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls. Inc.
Concentration: /,# /
pH Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
Concentration: /
pH Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
Concentration: control
Batch #
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
_______Hardness
Alkalinity
0-1
^.2-2-
7.5Z')
7.77
iSz
s.
w
TTT
ze./
0-1
■?.iz
7M
7^
2.^2
v-i.j
233
TSO
z.o!
0-1
7. TQ
7.^
AT.3
2Q-?
Z/S
^0
—
2-3
g,l?
7 7/
^.10
Day
3-4
8, l $
, 7-
T’fo
-z YA
7lolp
5-6
ffl?/
~1.7T
7?oMTU 4 a
277
zc./
-
52-^
5-6
MU
77^
(7 3'3
127
2S?
2 Ci./
Day
3-4
53(3-
?. IS
KM 7-SSL
7.^
2/3
TAG
954
-^■1
Yr-
57-
1-2
7.76
(.35
^iio
sqY
to. !
4-5
U_____
2:. '36
2-3
_Ki£
7^i
7^
~^0
2^^
•^3
I
1-2
g.23
7.50
5(tY
277
2-gO
y-ZA TH
\20.1 ^(j.\
6-7
_£U_
782
767'
M
■2 5.0
1SF~
7. /
y.23
7.~?y
■7.SD
Ml
277
240
zz-7
<(0. /
6-7
7-83
7.(J, t^T
uCb
^"4 5
^c. I
4-5
7,31
7. TO
5.1(1
-/CT
2- 5
'^3
zc,/
5-6
?.6y
zs7
24
23^
2S?
loT
Day
3-4
^IS
773
7.54 TVT
W
AeU
Zi?. I
'Z^.O
an
3-Z-fo
Chemical and Physical Determinations
Client:^ jflUi joO_________ NPDES#: MGQQZ^^ Start Date: ix/p///
1 est Organism. Pi m eph ales promeias Analyst(s): End Date: / z. !>1
4-5
'%30
7.^
7.loX
7.^
77.7.
2S.0
WT
•^7
ao7 I
Chemical and Physical Determinations
Remarks
D.O.
Temp.
Conductivity
5-6 Remarks
D.O.
Temp.
Remarks
D.O,
Temp,
A
6-7 Remarks
4-5
ft,
Day
3-4
2-3
9.20
Concentration: 100%
__________________pH
______ Conductivity
Residual Chlorine
_______ Hardness
________ Alkalinity
Concentration: fi X
pH ___ Initial
Final
Initial
Final
Initial
Final
Conductivity InitfMgh^
_________ Final
Residual Chlorine
Concentration: t-/ 7,
pH Initial
Final
Initial
Final
Initial
Final
Conductivity1 Initial
__________ Final_____
Residual Chlorine |/6>./
Concentration: ’Z
pH Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
0-1
7^?
7.$^
25-.3
3^ '
2^1
o-i
?.2 3
7.^
Day
3-4
5-6
2^
741
I
(o.&
2^3
£7.7
214
■<o.)
1-2
7.^0
2S0
ss 7
73?
3^3
Z i?./
NPDES#: rn ^"75'3
Analyst(s): $r
Day
3-4
7,SB
77fc 7^
zy.> 7rr~
7-^0
2g.I
4-5
S'.yi
773
7,o?
T-r.o
23.0
J3__
■^7/
2b-1
5-6
? 2-0
7? /
7
37T
2C. I
2-3Hz
737
7.7?
2y.r
< a.
2-3
7.g?
?.yo
ICWSeKM.r
zy.r
.34,/
37F
4-5
7,9-g
2^L
-2.5'72
2C. I
7.77
2^
27.0^ 9. 127
346
2C,\
1-2
’ <5, ?b
~ 7>G>%
7.7<
27.7
-^71^1
|
1-2
^3^
7.77
67^
277
M(b
2-77
316
7.9?
77.^
^?r.
"64
26 £
2^7
Page 2 of2
Time: 371^_
Time:
ivjeritech, trufc:.
4-5 5-6
1 7
AQ, |
4^
Start Date: '^/(f//.
End Date: /z-Zj /n
0-1
7'%
7. Sb ~t7r
273
^.7 _
'7-73 &
^0.
6-7
2/ ?,^o‘
----- 7^
27.p
-±±L
2-3____
M__
72.
7Q?
77>
3.tl pl
2577
wr
/ \2t>.!
6-1
' ?.19
T.7<
2.7 0
2^
an
^C. /
6-7
7ii
^0 '
Client: Clau+nn
Test Organism: Pimephales promelas
0-1 | 1-2
78 b
MO
^0-1 ,
___43L_
1.7:
7.47,
^.r
IW TiHi
i<a /1
Day
3-4
S’./y
in,
L22_
Facility:
Outfall:
Organism: Pimephales promelas
CONC REP # ORG.SURV %
ID I (SoA
O.b£> IGoioB
89uA 898.90 Qi /Dc /OP
S.Bl81 U>.95 IV, D(oo (Ot>
8?)C-95Sm.u:~9 Co.. 5^O.U6^ivA luo
&9O,\v5 saio.xi kMc'X /oB ICfO
O.IOJg/Oc /OP
^93-. 85 %-Cl 0. aO 9DfD IDQ
^.40 040/OA /DO
1.0/889.95 5,.0~ c ICB 0.. LcrtC /LG
u. c %3G8O 5 .Oto ID 0. (c>h(.cc /Ld
SIjJP ^80. CO /(jlDJ-7 JO -
PAN WT.
(mg)
FISH LARVAL SURVIVAUGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
MEAN
WT./ORG. (mg
0
AJ
T
0
C JJi
MERITECH, fAfC.
ENVIRONMENTAL LABORATORIES
A Divilion of Wtl„ TochnoJogy and Controls, Inc
8ia,9o.
835
Page:l_ of 2
.54
Olau4nn_____
NPDES #: AJC £>0^5^53
Date of Test: //'
Initial weights taken on: 1
Final weights taken on:
Facility; Paga: of 2
Outfall:
Date of Test:
Organism: Pimephales promelas
CONC REP # ORG.SURV %
;croA
Wifi IOz.6) 7.0. WQ I JDB
5 ?DglrAP-c
£ A. 44 ID |C50,341D
o.USBCo .54 /OA ice-
^.0 /■SiSacO jo 0.3G54.45B 10O
334,51 C \0iR, 0; i /D ISOc
§35. oA o. 19*4D /CD
2 mi QJM?4 ,14 /DA /CD
y 15.53 4.3 i 4 l&0 /toB
ml cm[0c /CD
1 /(inoD
PAN WT.
(mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF ORG.
(mg)
MEAN
WT./0RG. (mg)
^tc. o'
m I T'/B
PAN + ORG.
WT. (mg)
4T43
by:
by: S~T
MERITECH, INC.
ENVIRONMENTAL LABORATORIES
-A Division of Water Technology and Controls, Inc.
10 ■.55 5
NPDES #: AJC 002-5763
iiAA—__
Initial weights taken on: Q
Final weights taken on: IrVVn I \
2-14 AB
215.54
245,35
£545
■sr©-'I5
End Time:
Concentration Confco/
Day 0 Day 1 Day 3 Day 4
o0Rep#1
0Rep #2
0 C>Rep #3
0o 0 0 0oRep #4
Concentration
Day 5
0 0 0Rep #2
0ooRep #3 O
0 0oo
Concentration |, 0 '/.
Day 2 Day 4 Day 5 Day 6 Day 7
Rep #1 0
0
0
D 0
o
0
0_
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Day 2 Day 6
Day 4
a
Day 2
D
Day 3
0
Day 0
Day 5
0
Day 3
0
0
Day?
a
o
Day 6
Day 7
D
0
0.5 '/■
Day 0 Day 1
Rep #1 Q D
0
Day 1
d__
Rep #2 0 0
Rep #3 0 O Q
Rep #4 Q Q
Client: (2, L Start Date: Start Time: 3
End Date:
Rep #4 C>
Concentration "2 iO
Day 0 Day 2 Day 3
Rep#1
0 0o Rep #2
0 0
00
Concentration
Day 0 Day 1 Day 2 Day 6 Day 7
<0
Rep HA O o
Concentration (3,()
Day 0 Day 1 Day 3 Day 5 Day 6 Day?
’ I
O 0Rep #1
0 0oRep #2
0Rep #3 o
0 0Rep #4 '0
_0_ _O_
0 Q
0 C)
o
0
■3^
A
Q_
0.
0
Rep#1 Q Q
Rep #2 O 0
Rep #3 Q 0 $
Day 4
0
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Day 1
Day 3
0
Day 4
o
Day 4
0
Day 5
0
Day 7
0
Day 5
0
Day 6
0
Day 2
0
o
0
0
0
d
ztrid
Rep #3 0
Rep #4
Client:Start Date: l'2'/^ // Start Time:.
End Date: 1 End Time:
I2DU4Meritech Sample ID #;
State:Zip:
SAMPLE INFORMATION
# of containers:
Sampling Time: Start Date.
End Date:
AIWC:%
Comments:
Time:
Time:
Time:
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM PM
Sample Temperature (°C):
3:/oDate' Time:AM
'_______I Sample Condition:
YELLOW = Cliant copy
Client:
Contact Person:
Address:
City.;
County:
Test Required: gf Chronic (7 days)
Acute (24-48 hours)
PM
MERITECH, INC.
Bloassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
Email: [e-mail]
JL2. - -^2 / /
(Z - G - a e> / (
Fax: [phone]
Web Site www.meritech-labs.com
WHITE = Laboratory copy
Relinquished by:
Received by: '
Relinquished by:
Received by. \
Relinquished by:
Received by:
Relinquished by:
Received by:
PO#:
NPDES#: NC ° Q 2.
Phone:
Pipe#■ pg (
A 2 7^
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
o airspace), CHILLED AND COVERED WITH ICE ***
Signature:
Samp,B Site: —
Sample Type: Grab Composite
Relinquished by:
Received by.
Sample Temperatures (°C): /■ Z / /. L,
/(.'SO , AM _ PM
3 )<? am

SHIPPING INFORMATION
Da,e -
Date: Z /
Date: / 7,-4 ~ / /
CLIENT INFORMATION
_______
1670
£;/CA-'l'
tf'ak tl 3 J"#
Method of Shipment: UPS FedEX Meritech Pick-up
Rampies sh.pped on Friday must be FedEx and must be clearly labeled for Saturday delivery »
SAMPLE RECEIVING (Laboratory Use Only)
Start Time. PM
End Time: /0-./5'~ AM PM
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (n<
Collector-, Nama; Print
TOXICITY TEST INFORMATION
Test Organism: Cerlodaphnla dubia (water flea)
X Pimophales promelas (fathead minnow)
Q Mysldopsis bahia (shrimp)
Test Concentrations (if multiple dilutions);
SAMPLE INFORMATION
S* Composite
# of containers:
Sampling Time: Start Date.Start Time:
End Date:
IWC: %
Comments:
PM
(AM PM !
AM 'M
AM PM
Date: Time: AM PM
Date- Time: AM PM
Date: Time: AM PM I
Date: Time: AM PM
/2.-8J!W30Date:Time:AM
/ / Sample Condition:
BELLOW = Client copy
37
36>
Relinquished by:
Received byf
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by: _
Client:
Contact Person:
Address.
City:_
County:
Sample Site:
Sample Type: Grab
Time:
Time:
Time:
Time;
Test Required: Ixf Chronic (7 days)
Acute (24-48 hours)
MERITECH, INC.
Bloassay Sample Chyin of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]^748
Eniail: [e-mail]
Fax: [phone]
Web Site: www.meritech-labs.com
J g /t H
WHITE * Laboratory copy
PM-;
PC#:
NPDES#: NC
Phone: y 3~
Pipe#:
client information
S-t-.
Sample Temperature (°C):
Method of Shipment: UPS
" Samples shipped on
Relinquished by:
Received by:
Sample Temperatures (°C): I . j / |, |
Merited! Sample ID #: 120% 45
FedEX Meritech Pick-up
Friday must be FedEx and must be clearly labeled for Saturday delivery »
SAMPLE RECEIVING (Laboratory Use Only)
AM PM
TOXICITY TEST INFORMATION
Test Organism: Ceriodaphnla dubia (water flea)
Plmephales promelas (fathead minnow)
O Mysidopsls bahla (shrimp)
Test Concentrations (if multiple dilutions):
JAZZ///
11---------- End Time. AM PM ------ CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
“ SAMPLE CONTAINERS ARE TO BE COMPLETEL Y FULL (no air space), CHILLED AND COVERED WITH ICE -
Coliector's Name: Print:Signature:
SHIPPING INFORMATION
Date: / ) $7/) f
----- Date: I
— Date: /1 \
Date;
Meritech Sample ID #:
CLIENT INFORMATION
Sample Site:
# of containers:
Sampling Time: Start Date:
AM PM
IWC:%
Comments;
Date:
Date:Time:
Date: Time: AM
Date: Time: AM PM
Date: Time: AM PM
Date: Time: AM IPM
Date: Time: AM PM
Sample Temperature (°C):
Date: Time:AM PM
/ Sample Condition:
WHITE = Laboratory copy YELLOW - Client copy
2.' / 5
Relinquished by:
Received by:
Relinquished by: /
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Time:
Time:
Test Required: ££ Chronic (7 days)
Acute (24-48 hours)
Fax: [phone]
Web Site: www.meritech-labs.com
1^7-^ If
/'T-^-Zolr
Start Time:
End Time:
SJ./S'/A Of (
End Date: AZ/?/*
TOXICITY TEST INFORMATION
Test Organism: JSJ Ceriodaphnia dubia (water flea)
P/mephales prometas (fathead minnow)
O Mysidopsls bahla (shrimp)
Test Concentrations (if multiple dilutions):

MERITECH, INC.
Bioassay Sample Chqin of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone]
Email; [e-mail]
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
'O air space), CHILLED AND COVERED WITH ICE ***
Signature:
PO#:____________________
NPDES#: NC
Phone:
Pipe #: QO? j
ZiP-
State /J
SAMPLE INFORMATION
ZF U<J) /fZ-T-
Sample Type: Grab ^Composite
Client: of
Con,ac' pers°":
Address f)? //ooc S-h-
c'ty
County: 'To k
Relinquished by: B
Received by: MJ ft,
Sample Temperatures (°C): [.4 / [4 f
’** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (n>
Collector's Name: Print: 2^;^
-
Me,hoc,ofShiPmenl:D UPS FedEX Meritech Pick-up
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery "
SAMPLE RECEIVING (Laboratory Use Only)
AM PM
SHIPPING INFORMATION
Date: // : Jg -AM—PM
//- 3/^ ?AM> PM
AM £j\F
PM
Transform:NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
Data PASS normality test (alpha = 0.01). Continue analysis.
0.0748
0.9527
= 0.8840 (alpha = 0.01 ,
= 0.9160 (alpha = 0.05 ,
N = 24)
N = 24)
Title:
File:
Critical W
W
D =
W =
clayton
clayton
Transform:NO TRANSFORMATION
Calculated Bl statistic =2.2945 (p-value = 0.8071)
Data PASS Bl homogeneity test at 0.01 level.Continue analysis.
Critical B = 15.0863
= 11.0705
Title:
File :
5)
5)
clayton
clayton
Bartlett's Test for Homogeneity of Variance
(alpha = 0.01, df =
(alpha = 0.05, df =
Transform:NO TRANSFORMATION
ANOVA Table
SOURCE DF SS MS F
Between 5 0.0259 0.0052 1.2443
Within (Error)18 0.0748 0.0042
Total 23 0.1006
(p-value = 0.3298)
Critical F
Since Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)
Title:
File:
= 4.2479
= 2.7729
df = 5,18)
df = 5,18)
(alpha = 0.01,
(alpha = 0.05,
F <
clayton
clayton
Transform:NO TRANSFORMATION
Dunnett's Test TABLE 1 OF 2 Ho:Control<Treatment
GROUP IDENTIFICATION T STAT
Dunnett critical value = 2.4100 (1 Tailed, alpha = 0.05,df = 5,18)
Transform:NO TRANSFORMATION
Dunnett's Test TABLE 2 OF 2 Ho:Control<Treatment
GROUP IDENTIFICATION
Title:
File:
Title:
File;
TRANSFORMED
MEAN
DIFFERENCE
FROM CONTROL
SIG
0.05
1
2
3
4
5
6
NUM OF
REPS
4
4
4
4
4
4
0.7658
0.6957
0.6933
0.6855
0.7530
0.6878
MIN SIG DIFF
(IN ORIG. UNITS)
0.1098
0.1098
0.1098
0.1098
0.1098
MEAN CALCULATED IN
ORIGINAL UNITS
0.7658
0.6957
0.6933
0.6855
0.7530
0.6878
% OF
CONTROL
14.3
14.3
14.3
14.3
14.3
1.5358
1.5906
1.7607
0.2797
1.7113
0.0700
0.0725
0.0803
0.0127
0.0780
1
2
3
4
5
6
control
. 5
1
2
4
8
control
. 5
1
2
4
8
clayton
clayton
clayton
clayton
Transform:NO TRANSFORMATION
Shapiro - Wilk’s Test for Normality
I
Title:
File:
Critical w
W
D =
W =
clayton - survival
claytonsurv
0.0075
0.4651
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning - The first three homogeneity tests are sensitive to non-normality
and should not be performed with this data as is.
= 0.8840 (alpha = 0.01 , N = 24)
= 0.9160 (alpha = 0.05 , N = 24)
Transform:NO TRANSFORMATION
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
Title:
File:
clayton - survival
claytonsurv
i
i
i
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.
c
Sludge Management Plan
Introduction:
Process Description
The sludge conditioning and disposal systems consist of: two drum thickeners (80 gpm &
300 gpm), polymer flocculation, two .36 mg aerated holding tanks, one .09 mg aerobic
digester, and sludge transfer pump.
The plant consists of a mechanical and manual bar screen, Jeta (Vortex) type grit removal
system, 9 inch parshall flume with flow meter, and a composite sampler. The influent
pumping station has three 60 hp dry pit submersible pumps and one 40 hp pump.
Biological nutrient removal is achieved by Carrousel Denite R System (Eimco), the
system contains an anaerobic selector, two anoxic tanks, and two oxidation ditches. The
biological system is controlled by a PTC based control system that monitors oxygen
throughout the system. The plant also has three circular clarifiers, two mixed bed tertiary
filters, ultraviolet disinfection, effluent pumping station, in plant reuse pumping system,
and a reclaim water pumping station. In addition to the biological systems, the plant has
chemical feed for alkalinity (magnesium hydroxide), alum precipitation (alum sulfate),
and flocculation (polymer).
The Town of Clayton owns and operates a 2.5 mgd water reclamation facility. The
facility is named: Little Creek Water Reclamation Facility and operates under NPDES
Permit # NC0025453. The facility also delivers reclaim water to the Pine Hollow Golf
under Permit # WQ0022224. The facility is also covered under Stormwater Permit
#NCG 110000.
Sludge Management/Maintenance Plan
Little Creek Water Reclamation
Town of Clayton, NC
NPDES Permit # NC0025453
The Town of Clayton owns and operates a municipal wastewater collection and treatment
system, reclaim water, pretreatment program, operates a certified laboratory and
maintains a land application program. Wastewater processed by the treatment facility
originates from domestic and industrial sources. The approximate ratios of these sources
are seventy percent domestic and thirty percent industrial. The Town also has inter local
agreements with Johnston County Utilities and the City of Raleigh to treat wastewater
from the area. This sludge management plan, as required by the NC Division of Water
Quality, outlines plant processes and management procedures to ensure biosolids disposal
meets both state and federal requirements.
Sludge Disposal Options:
Responsible Persons:
Sampling Plan:
Sample Requirements:
Annual Sampling:
Location: Sludge Holding Tanks
Aluminum, Ammonia-Nitrogen, Arsenic, Cadmium, Calcium, Copper, Lead,
Magnesium, Mercury, Molybdenum, Nickel, Nitrate-Nitrite Nitrogen, Phosphorus,
Potassium, pH, Plant Available Nitrogen (by calculation), Selenium, Sodium, Silver, %
Total Solids, TKN, Zinc.
Quarterly Samples:
Location: Sludge Holding Tanks
The following sampling protocols shall be followed. All samples will be collected in
clean and/or sterilized containers provided by the contract laboratory. Chain of custody
information is required for each sample collected (see attachment one). Sampling
equipment shall be cleaned prior to and after sample collection. Sampling equipment
used to collect fecal coliforms shall be sterilized prior to sample collection; all others will
be cleaned with laboratory grade detergent and rinsed with deionized water.
I he land application operator in responsible charge is responsible for ensuring that the
sampling and maintenance functions are carried out as outlined in the land application
permit.
The Town is also included permitted at the Johnston County Landfill for dewatered solids
and also maintains an agreement with Johnston County Utilities to dewater thickened
biosolids at the landfill site. The Town may also use McGill Environmental for
composting at its Roseboro or New Hill composting site.
Corrosivity, ignitability, reactivity and Toxicity Characteristics Leaching Procedure
(TCLP)
The Town maintains a Residuals Land Application Permit # WQ 0023178. The permit
allows up to 400 dry tons per year of Class B on local farm land. Additional capacity is
available through the use of dewatered solids disposal and off site dewatering.
39
31
28 Plus 0.09
27
26
25 0.00
24 Minus 0.03
23 Minus 0.06
Plant personnel will document and use the following chart for the temperature correction
for all pH readings.
Fecal colifomis will be collected from the sludge holding tank form the walkway using a
sterilized stainless steel dipper. This walkway is the only safe means to sample this tank.
The seven samples will be collected prior to disposal. Plant staff should request a five-
day turn-around on all samples collected and be aware of the short holding times of 6
hours. In order to meet Class B requirements, the geometric mean must be less than 2
million (MPN/gram TS, or CFU/gram TS). If the counts exceed the regulatory limits the
biosolids may not be land applied until the limits are met.
Nutrient, metals, and TCLP analysis contained in the land application and composting
permits will be collected from the sludge holding tank via a clean dipper using a four
sample composite method. The aliquots shall be collected from the walkway at two hour
intervals. The aliquots should be ~ 800 milliliters and placed in a gallon jug in the
laboratory refrigerator. After the fourth sample is added the sample should be well mixed
and transferred into the proper sample containers. A chart is provided on specific sample
requirements. Temperature and pH are grab samples and the pH shall be temperature
corrected.
pH Temperature Correction Factors at Varying Temperatures from Standard
(25°C)
Plus 0.15
Plus 0.12
33
32
30
29
38
37
Plus 0.06
Plus 0.03
36
35
34
Plus 0,42
Plus 0.39
Plus 0.36
Plus 0.33
Plus 0.30
Plus 0.27
Plus 0.24
Plus 0.21
Plus 0.18
Correction Factor
Plus 0.45
Temp°C
40
Vector Attraction Reduction:
Pathogen reduction:
Spill control and prevention:
Pathogen reduction shall be qualified by Fecal Coliform testing from the holding tanks. A
minimum of seven samples will be collected per quarter based upon permit requirements.
Sampling is not required when no events occur during the quarter.
Alternatively a specific oxygen uptake may be performed if the solids concentration is
less than two percent and enough digestion time has been provided, a spreadsheet
provided by the land application unit shall be used for the calculations, a minimum of
seven samples must be taken.
Vector attraction reduction will consist of alkali addition directly into either sludge
holding tank. Prior to land application a contractor will be used to supply calcium
carbonate at a level to achieve a ph of > 12 units for two hours and maintain a pH of >
11.5 tor an additional 22 hours. There will be no additional material added to the tank
until the land application is complete. A log sheet will be maintained on-site and will
contain the following information: date, times, location, purpose, amount of material
added, initial pH. pH after addition, pH two hours after addition, pH twenty two hours
after addition, names of persons involved, completion date and volume of biosolids land
applied. If the required parameters are not met the process must be successfully
completed prior to land applying the sludge. Note: It is a good practice to perform ajar
test to the contents of the tank prior to treatment, in order to determine the required
amount of alkali to add.
Minus 0.09
Minus 0.12
Minus 0.15
Minus 0.18
Minus 0.21
Minus 0.24
Minus 0.27
Minus 0.30
Minus 0.33
Minus 0.36
Minus 0.39
Minus 0,42
Minus 0.45
22
21
20
19
18
17
16
15
14
13
12
11
10
The Town’s hauling contractor is required to have a spill control/ prevention plan.
Inspections:
Should the sodium adsorption rate (SAR) of ten (10) or higher be encountered, the Town
will utilize the services of a registered soil scientist to make recommendations to
maintain soil quality.
This is a working document; it must be reviewed periodically for completeness and
efficiency and does not replace the requirements in the various government standards and
permits.
The land application ORC is required to ensure that the permit conditions are being met
by plant personnel and the sludge application contractor. During each application event
the ORC or backup ORC will visit the sites in use.
Land application rates are based upon Realistic Yield Expectations based upon
information acquired from the North Carolina Department of Agriculture and Consumer
Services. The rates are used by the Town’s contracted soil scientist who prepares
quarterly application summaries and the annual report.
The facility has a spill control plan as required by the facility’s storm water permit. The
sludge loading area has a contoured drainage area that empties into an in plant recycle
system.