NC0025453_Permit renewal application_20071231

I
Legend wWastewater i Effluent
— — Sludge
Decant / Filter Backwash / Bed Underdrainage
Scum
(2J mad)
Anaerobic Selector Tank
rii zII ENGINEERING
O PLANNINGI
►ARCHITECTUREClerigesterI
II (3.026 mgd)I (5.0 mgd)I Dist. Box (1.67 r.iQd)PS.I II ■>T
Ox. Basin No. 1I'(1.074 mgd)
Filter BackwashP.S. I I
I
►I P.S.
First Stage Anoxic Tank Drain r*□
(1.67 myj)Sludge Recirc.Influent
~l
]T.F.P.S.Influent
aI' ' (2i mod)
(2^ mpd)
Q (2.5 mpd)
V
Eff. Pump Sta.— Decant
I
EXHIBIT 1
Schematic Flow Diagram of Clayton LCWRF
I
*
To Neuse River
I
I
♦
I
! i
5
I
_ _ p- —
P.S.
1
Secondary
Clarifier-.
Aerobic
Digester
— Sludge
Drying Beds
THE
WOOTEN
COMPANY
Trickling
Filter -
UVDisinf/
Chlorination ■
Dechlorination
Clarifier
No. 1-^
Flow Balances at
Design Average Daily Flow
z/Clarifier
No. 2—,
Clarifier
No. 3
i
11 I
1
^7
Sludge
Stabilization/
Storage Tank
Sludge
Drying Beds
Chem Feed
& Sludge
Thick. Bldg.
Sludge
Loading
Sta.----
Existing Process Train
is Abandoned
’“’I
A
?l H
-II P.S.
□ D.B.
Influent
Pump Sta.
Clar. Dist. Box
1 Scum -
— First Stage Anoxic Tank
/ /—Ox. Basin No. 2
>X
Sludge
Thickening Bldg.
1 I
Chemical
Stor. Area----
Screening
& Grit Rem.
(0.83 mgd)
|(Zs myd)
/—Tertiary
/ Filters
T~TM
Sludge
Stabilization/
I
I
I
I
I
LEGEND
PROCESS FLOW
RAS FLOW
WAS FLOW
WOOTENPIPE
COMPANYWATER PIPE
STRUCTURE
FENCE
PLANNING
TREE LINE
s
AERATION BASIN NO. 2
TANK
BUILDING
EXHIBIT 2
PROCESS FLOW DIAGRAM OF CLAYTON LCWRF
TtRTIARY
FILTERS
ANOXIC
TANKS
liIHLOHINE
iXiNTACT
CHAMBER
SCUM
PS —
RETURNSLUDGE
PUMPSTATION
SECONDARY
CLARIFIER NO. 1
SECONDARY
CLARIFIER NO 3
■ uftth
VMAT
SECONDARY
CLARIFIER NO 2
ANAEROBIC
SELECTOR
TANKS
SLUDGE
DRAW-OFF
VAULT
12-DI
FORCEMAIN
EFFLUENT
PUMP STAT.
I SLUDGE '
STABILIZATION / HOLDING
\ TANK No 1 /
SLUDGE
.STABILIZATION / HOLDING
I TANK No 2 I
DRYIN 5
BEDS
—- T WATER
LINE
SLUDGE
P.S.
SCUM
< PS.^.
L HEADWORKS
EX. INFLUENT
PUMP STATION
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uniKWfcK cgn wi-jgw i m n “v
g
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Ch
3
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£
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^2£
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£
U4
LEGEND
EFFLUENT FORCE MAIN
EFFLUENT OUTFALL
INFLUENT SEWER
LITTLE CREEK WRF
USGS QUAD: CLAYTON, NC 2002/o
zo
SCALE: 1 "=2000l
RECEIVING STREAM: NEUSE RIVER
RIVER BASIN: NEUSE RIVER BASIN
POINT 001
LAT. 35*39' 50"
LONG. 78*25' 26"
LITTLE CREEK
WATER RECLAMATION
FACILITY
TOWN OF CLAYTON
LITTLE CREEK WATER
RECLAMATION FACILITY
NPDES PERMIT NO. NC0025453
>1-
i. .Jr'
|i -n. '>*’•' K4Vx‘Ji! it
s- *'
THE WOOTEN COMPANY
ENGINEERING PLANNING ARCHITECTURE
120 North Boylan Avenue Raleigh NC 27603-1423
919.828.0531 fax [phone]
EFFLUENT
OUTFALL
//s^S f’ ■w
EFFLUENT
x FORCEMAIN
-
- .
A y
V//(A
' ' W:<iP
zxj'/ W; w u A-
Al
■'
^4a3aa-
|v A
AAAA.AAA
- J
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AV'-AA A bSA. AbA
caaaaA: - A
EXISTING 24"
INF. SEWER
\ z. .//tA . L
EXISTING 18"
INF. SEWER
A I M
-4
October 9, 2007
Dear Mr. Biggs:
Sincerely,
ftcc:
NorthCarolinaNaturally
An Equal Opportunity/Affinnative Action Employer - 50% Recycled/10% Post Consumer Paper
R STEVEN BIGGS
TOWN MANAGER
TOWN OF CLAYTON
PO BOX 879
CLAYTON NC 27520
North Carolina Division of Water Quality
Internet: www.ncwaterqualitv.org
William G. Ross Jr., Secretary
North Carolina Department of Environment and Natural Resources
Coleen H. Sullins, Director
Division of Water Quality
Customer Service
1-877-623-6748
Subject: Receipt of permit renewal application
NPDES Permit NC0025453
Little Creek Water Reclamation Facility
Johnston County
CENTRAL FILES
Raleigh Regional Office/Surface Water Protection
NPDES Unit
Dina Sprinkle
NPDES Unit
Michael F. Easley, Governor
The NPDES Unit received your permit renewal application on October 5, 2007. A member of the
NPDES Unit will review your application. They will contact you if additional information is required to
complete your permit renewal. You should expect to receive a draft permit approximately 30-45 days before
your existing permit expires.
If you have any additional questions concerning renewal of the subject permits, pie:
Nowell at [phone], extension 512.
£
:ase contact Jackie
ICU
^0^?
co / 111 I
fr
.U
im
OCT I ' 2007
1617 Mail Service Center Raleigh, NC 27699-1617 Phone [phone]
Location: 512 N. Salisbury St. Raleigh, NC 27604 Fax [phone]
I
I
I Town of Clayton, North Carolina
I
October 2007
I
OCT I I 2007I
L
I i2!l North Boylan Avenue Raleigh NC 27603-1423
'■>!') 828.0531 ’ax919.83.1 J589
8
THE WOOTEN COMPANY
ENGINEERING PLANNING ARCHITECTURE
iDjl ttl
--------- HN3(]
100 1
ILffi fl j[_ji j] [uj
Application for Renewal of
NPDES Permit No. NC0025453
Pnemle/i CamtuMtuitj,/JcZiue. ‘Pa^dleA
October 3. 2007
Re.
Dear Mrs. Candelaria.
I
If you have any questions or require any additional information, please do not hesitate to contact me at 919-553-5002.
Sincerely.
c:
P.O. Box 879 • Clayton. North Carolina 27528 • Office [phone] • Fax [phone]
CAA!,
Enclosures
Charlie Davis, The Wooten Company
R. Steven Biggs
Town Manager
Robert Ahlert
MAYOR PRO TEM
Bruce Thompson
TOWN ATTORNEY
Steve Biggs
TOWN MANAGER
Jody L. McLeod
'mayor
Ms. Frances Candelaria
NC DENR/DWQ/Point Source Branch
1617 Mail Service Center
Raleigh. NC 27699-1617
1.
2.
3.
4.
5.
6.
7.
Request for Permit Renewal
NPDES Permit No. NC0025453
Town of Clayton. North Carolina
COUNCIL MEMBERS
Bob Satterfield
Alexander R. Atchison
Alex Harding
Michael Grannis
Enclosed, please find one (1) original and two (2) copies of an application package requesting renewal of the NPDES Permit
for the Little Creek Water Reclamation Facility (LCWRF) located in Clayton, North Carolina. Each application package
contains the following:
EPA NPDES Form 2A
Topographic Site Map
Schematic Flow Diagram
Process Flow Diagram
Three (3) Priority Pollutant Analyses
Four (4) Chronic Toxicity Tests
Sludge Management Plan
The Little Creek Water Reclamation Facility has undergone one major modification at the facility since issuance of the most
recent permit. In November 2005 construction began at the plant to provide enhanced biological nutrient removal and
improved biosolids handling capabilities. The project includes construction of an anaerobic basin, two (2) pre-anoxic basins,
modifications to the two existing aeration basins, sludge thickening equipment and a sludge stabilization/storage basin.
Construction of the new improvements should be completed by early November 2007.
In 2006 Clayton purchased 3,668 Ib/yr of estuary total nitrogen allocation from SGWASA. This allocation was placed in
reserve as noted in the Town's individual NPDES pennit as well as in the Neuse River Compliance Association (NRCA) and
its co-permittce member’s NPDES permit
Currently, the Town is completing the purchase of 1,645 Ib/yr of estuary total nitrogen from Unifi Kinston. LLC. Based upon
the Town’s transport factor of 50%, 1.645 Ib/yr of estuary allocation results in 3,290 Ib/yr of end of pipe total nitrogen at the
LCWRF discharge. Out of tills 3.290 Ib/yr we request that 1,432 Ib/yr of the end of pipe allocation be added to the cunent
21.400 Ib/yr included in the Town’s individual NPDES permit. As a result lire Total Nitrogen Load will become 22,832 Ib/yr
at tlie end of pipe (i.e. 2.5 mgd x 3 mg/L TN x 8.34 x 365 days/yr = 22.832 Ib/yr rounded). In addition we request that the
remaining 1,858 Ib/yr be placed in reserve in the Town’s individual NPDES and noted in the NRCA NPDES permit similar to
the action granted in the SGWASA case. This will place the Town’s total nitrogen end of pipe allocation held in reserve at
9,194 Ib/yr (i.e. 7,336 Ib/yr + 1.858 Ib/yr =9.194 lb/_vr end of pipe or 4.597 Ib/yr at estuary').
I
APPLICATION OVERVIEW
BASIC APPLICATION INFORMATION:
A.I
B.
C.Certification. All applicants must complete Part C (Certification).
SUPPLEMENTAL APPLICATION INFORMATION:
D.
1.
2.
3.
E.
1.
2.
3.
F.
1.
2
a.
I b.
c.
I G.
ALL APPLICANTS MUST COMPLETE PART C (CERTIFICATION)
I EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 1 of 24
I
I
Basic Application Information for all Applicants. All applicants must complete questions A.1 through A.8. A treatment works
that discharges effluent to surface waters of the United States must also answer questions A.9 through A. 12.
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
FORM
2A
NPDES
NPDES FORM 2A APPLICATION OVERVIEW
. ■
Toxicity Testing Data. A treatment works that meets one or more of the following criteria must complete Part E (Toxicity Testing
Data):
Has a design flow rate greater than or equal to 1 mgd,
Is required to have a pretreatment program (or has one in place), or
Is otherwise required by the permitting authority to submit results of toxicity testing.
Industrial User Discharges and RCRA/CERCLA Wastes. A treatment works that accepts process wastewater from any
significant industrial users (SIUs) or receives RCRA or CERCLA wastes must complete Part F (Industrial User Discharges
and RCRA/CERCLA Wastes). SIUs are defined as:
All industrial users subject to Categorical Pretreatment Standards under 40 Code of Federal Regulations (CFR) 403.6 and
40 CFR Chapter I, Subchapter N (see instructions); and
Any other industrial user that:
Discharges an average of 25,000 gallons per day or more of process wastewater to the treatment works (with certain
exclusions); or
Contributes a process wastestream that makes up 5 percent or more of the average dry weather hydraulic or organic
capacity of the treatment plant; or
Is designated as an SIU by the control authority.
Combined Sewer Systems. A treatment works that has a combined sewer system must complete Part G (Combined Sewer
Systems).
Expanded Effluent Testing Data. A treatment works that discharges effluent to surface waters of the United States and meets
one or more of the following criteria must complete Part D (Expanded Effluent Testing Data):
Has a design flow rate greater than or equal to Imgd,
Is required to have a pretreatment program (or has one in place), or
Is otherwise required by the permitting authority to provide the information.
Additional Application Information for Applicants with a Design Flow £ 0.1 mgd. All treatment works that have design flows
greater than or equal to 0.1 million gallons per day must complete questions B.1 through B.6.
Form 2A has been developed in a modular format and consists of a “Basic Application Information” packet
and a “Supplemental Application Information” packet. The Basic Application Information packet is divided
into two parts. All applicants must complete Parts A and C. Applicants with a design flow greater than or
equal to 0.1 mgd must also complete Part B. Some applicants must also complete the Supplemental
Application Information packet. The following items explain which parts of Form 2A you must complete.
BASIC APPLICATION INFORMATION
PART A. BASIC APPLICATION INFORMATION FOR ALL APPLICANTS:
All treatment works must complete questions A.1 through A.8 of this Basic Application Information Packet
A.1.Facility Information.
Facility Name Town of Clayton - Little Creek Water Reclamation Facility
Mailing Address Post Office Box 879
Clayton, North Carolina 27520
Contact Person Steve Biggs
1 Title Town Manager
Telephone Number [phone]
Facility Address Durham Street Extension
(not P.O. Box)Clayton, North Carolina
A.2.Applicant Information. If the applicant is different from the above, provide the following:
Applicant Name Same as above
Mailing Address
Contact Person
Title
Telephone Number L 1
Is the applicant the owner or operator (or both) of the treatment works?
El owner El operator
Indicate whether correspondence regarding this permit should be directed to the facility or the applicant.
□ facility El applicant
A.3.Existing Environmental Permits. Provide the permit number of any existing environmental permits that have been issued to the treatment works
(include state-issued permits).
NPDES NC0025453 PSD
UIC Other
RCRA Other
A.4.Collection System Information. Provide information on municipalities and areas served by the facility. Provide the name and population of each
entity and. if known, provide information on the type of collection system (combined vs. separate) and its ownership (municipal, private, etc.).
Name Population Served Type of Collection System Ownership
Town of Clayton 12,126 Separate Municipal
East Clayton Industrial Area Industrial Separate Municipal
Total population served 12,126
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 2 of 24
RIVER BASIN:
Neuse
PERMIT ACTION REQUESTED:
Renewal
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
I A.5. Indian Country.
Is the treatment works located in Indian Country?a.
Yes 0 NoIb.
Yes 0 No
A.6.
Design flow rate 2^5.a.mgd
Two Years Ago Last Year This Year
I b.Annual average daily flow rate 1.341 mgd 1.473 mgd 1.232 mgd
Maximum daily flow ratec.3.885 mgd 2.088 mgd
A.7.Collection System. Indicate the type(s) of collection system(s) used by the treatment plant. Check all that apply. Also estimate the percent
contribution (by miles) of each.
0 Separate sanitary sewer 100 %
Combined storm and sanitary sewer %
A.8. Discharges and Other Disposal Methods.
Does the treatment works discharge effluent to waters of the U.S.?a.S Yes No
i.Discharges of treated effluent 1
ii.Discharges of untreated or partially treated effluent 0
iii.Combined sewer overflow points 0
iv.Constructed emergency overflows (prior to the headworks)0
Otherv.0
b.
that do not have outlets for discharge to waters of the U.S.?£3 No
If yes, provide the following for each surface impoundment:I Location:
mgd
Does the treatment works land-apply treated wastewater?c.El Yes NoIIf yes, provide the following for each land application site:
Location:Pine Hollow Golf Course
Number of acres:
0.243 mgd
I S intermittent?
d.
Yes 0 No
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 3 of 24
Annual average daily volume applied to site:
Is land application continuous or
Does the treatment works discharge or transport treated or untreated wastewater to another
treatment works?
Annual average daily volume discharge to surface impoundment(s)
Is discharge continuous or intermittent?
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
Does the treatment works discharge effluent to basins, ponds, or other surface impoundments
- -..................... w • • - - Yes
Does the treatment works discharge to a receiving water that is either in Indian Country or that is upstream from (and eventually flows
through) Indian Country?
Flow. Indicate the design flow rate of the treatment plant (i.e., the wastewater flow rate that the plant was built to handle). Also provide the
average daily flow rate and maximum daily flow rate for each of the last three years. Each year’s data must be based on a 12-month time period
with the 12 month of "this year” occurring no more than three months prior to this application submittal.
If yes, list how many of each of the following types of discharge points the treatment works uses:
I If transport is by a party other than the applicant, provide:
Transporter Name
Mailing Address
I Contact Person
Title
Telephone Number L I
For each treatment works that receives this discharge, provide the following:I Name
Mailing Address
Contact Person
Title
Telephone Number L 1
If known, provide the NPDES permit number of the treatment works that receives this discharge
Provide the average daily flow rate from the treatment works into the receiving facility.I mgd
e.
□ Yes C3 No
I If yes, provide the following for each disposal method:
Description of method (including location and size of site(s) if applicable):
Annual daily volume disposed by this method:
□ continuousIs disposal through this method □ intermittent?or
I
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 4 of 24
Does the treatment works discharge or dispose of its wastewater in a manner not included
in A.8. through A.8.d above (e g., underground percolation, well injection):
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
If yes, describe the mean(s) by which the wastewater from the treatment works is discharged or transported to the other treatment works
(e.g., tank truck, pipe).
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
WASTEWATER DISCHARGES:
I
A.9. Description of Outfall.
Outfall number 001a.
b.Location
(Zip Code)
Distance from shore (if applicable) c.ft.
d.Depth below surface (if applicable)3 ft.
Average daily flow ratee.1.384 mgd
f.Does this outfall have either an intermittent or a periodic discharge? Yes S No (go to A.9.g.)
If yes, provide the following information:
Number f times per year discharge occurs:
Average duration of each discharge:
Average flow per discharge: mgd
Months in which discharge occurs: I Is outfall equipped with a diffuser? Yes S Nog-
A.10. Description of Receiving Waters.
Name of receiving water Neuse Rivera.
b.Name of watershed (if known)Neuse Subbasin 03-04-02
United States Soil Conservation Service 14-digit watershed code (if known):
Name of State Management/River Basin (if known): Neuse River Basinc.
United States Geological Survey 8-digit hydrologic cataloging unit code (if known):03020201
d.Critical low flow of receiving stream (if applicable) 7Q10 = 186 cfs based on USGS data (summer 7Q10)
acute cfs chronic cfs
Total hardness of receiving stream at critical low flow (if applicable): e.mg/l of CaCO3
I EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 5 of 24
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
1
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Town of Clayton - Neuse River Between SR 1700 and NC Hwy 42
(City or town, if applicable)
Johnston
(County)
North Carolina
(State)
35'39'50"
(Latitude)
78'25'26"
(Longitude)
If you answered “Yes” to question A.8.a, complete questions A.9 through A.12 once for each outfall (Including bypass points) through
which effluent is discharged. Do not include information on combined sewer overflows In this section. If you answered “No” to question
A.B.a, go to Part B, “Additional Application Information for Applicants with a Design Flow Greater than or Equal to 0.1 mgd.”
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A.11. Description of Treatment
a.
E3 Advanced Describe:
b.
Design B0D5 removal or Design CBOD5 removal 98 %
Design SS removal 96 %
Design P removal 75 %
Design N removal 92.5 %
Other %
What type of disinfection is used for the effluent from this outfall? If disinfection varies by season, please describe:c.
UV Disinfection
If disinfection is by chlorination is dechlorination used for this outfall? Yes No
Does the treatment plant have post aeration? Yes No
minimum, effluent testing data must be based on at least three samples and must be no more than four and one-half years apart.
Outfall number:001
MAXIMUM DAILY VALUE AVERAGE DAILY VALUEPARAMETER
Value Units Value Units Number of Samples
pH (Minimum)6.5 s.u.
pH (Maximum)8.1 s.u.
3.89Flow Rate mgd 1.384 mgd 577
Temperature (Winter)7 "C 14 ’C 151
31 "C 23 •c 214
AVERAGE DAILY DISCHARGE
POLLUTANT ML/MDL
Cone.Units Cone.Units
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
BOD5 33.6 mg/L 3.3 mg/L 397
CBOD5 N/A N/A N/A N/A N/A
FECAL COLIFORM 6000 #/100 mL 18.95 #/100 mL 397
TOTAL SUSPENDED SOLIDS (TSS)97 mg/L 2.44 m9/L 396
EPA Form 3510-2A (Rev 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 6 of 24
END OF PART A.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
What level of treatment are provided? Check all that apply.
Primary Secondary
Other.
Indicate the following removal rates (as applicable):
PERMIT ACTION REQUESTED:
Renewal
Number of
Samples
ANALYTICAL
METHOD
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
BIOCHEMICAL OXYGEN
DEMAND (Report one)
Temperature (Summer)
_________* For pH please report a minimum and a maximum daily value
MAXIMUM DAILY
DISCHARGE
A.12. Effluent Testing Information. All Applicants that discharge to waters of the US must provide effluent testing data for the following
parameters. Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is
discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data
collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC reguirements of
40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a
BASIC APPLICATION INFORMATION
PART B.
B.1.
B.2.
b.
c.
d.
e.
Name:
Mailing Address:
Telephone Number:L 1
Responsibilities of Contractor:
I
b.
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 7 of 24
S No
telephone number, and status of each contractor and describe the contractor's responsibilities (attach additional
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
gpd
Briefly explain any steps underway or planned to minimize inflow and infiltration.
The Town of Clayton has an ongoing sewer system evaluation and rehabilitation program.
ADDITIONAL APPLICATION INFORMATION FOR APPLICANTS WITH A DESIGN FLOW GREATER THAN OR
EQUAL TO 0.1 MGD (100,000 gallons per day).
All applicants with a design flow rate 2 0.1 mgd must answer questions B.1 through B.6. All others go to Part C (Certification).
Inflow and Infiltration. Estimate the average number of gallons per day that flow into the treatment works from inflow and/or infiltration.
438,000
B.4. Operatlon/Maintenance Performed by Contractor(s).
Are any operational or maintenance aspects (related to wastewater treatment and effluent quality) of the treatment works the responsibility of a
contractor? Yes No
If yes, list the name, address,
pages if necessary).
B.5. Scheduled improvements and Schedules of Implementation. Provide information on any uncompleted implementation schedule or
uncompleted plans for improvements that will affect the wastewater treatment, effluent quality, or design capacity of the treatment works. If the
treatment works has several different implementation schedules or is planning several improvements, submit separate responses to question B.5
for each. (If none, go to question B.6.)
a. List the outfall number (assigned in question A.9) for each outfall that is covered by this implementation schedule.
001-Treatment plant upgrade to provide enhanced biological nutrient removal. The project includes construction of
an anaerobic basin, anoxic basins, modifications to the aeration basins, sludge thickening eguipment and sludge
stabilization/storage basin. Construction should be completed by early November 2007.______________________
Indicate whether the planned improvements or implementation schedule are required by local, State, or Federal agencies.
Yes 0 No
Topographic Map. Attach to this application a topographic map of the area extending at least one mile beyond facility property boundaries. This
map must show the outline of the facility and the following information. (You may submit more than one map if one map does not show the entire
area.)
a. The area surrounding the treatment plant, including all unit processes.
The major pipes or other structures through which wastewater enters the treatment works and the pipes or other structures through which
treated wastewater is discharged from the treatment plant. Include outfalls from bypass piping, if applicable.
Each well where wastewater from the treatment plant is injected underground.
Wells, springs, other surface water bodies, and drinking water wells that are: 1) within 7« mile of the property boundaries of the treatment
works, and 2) listed in public record or otherwise known to the applicant.
Any areas where the sewage sludge produced by the treatment works is stored, treated, or disposed.
f. If the treatment works receives waste that is classified as hazardous under the Resource Conservation and Recovery Act (RCRA) by truck, rail,
or special pipe, show on the map where the hazardous waste enters the treatment works and where it is treated, stored, and/or disposed.
B.3. Process Flow Diagram or Schematic. Provide a diagram showing the processes of the treatment plant, including all bypass piping and all
backup power sources or redunancy in the system. Also provide a water balance showing all treatment units, including disinfection (e.g.,
chlorination and dechlorination). The water balance must show daily average flow rates at influent and discharge points and approximate daily flow
rates between treatment units. Include a brief narrative description of the diagram.
If the answer to B.5.b is “Yes." briefly describe, including new maximum daily inflow rate (if applicable).c.I
d.
Schedule
Implementation Stage MM/DD/YYYY
- Begin Construction //11/08/2005
- End Construction 11/01/2007 /
- Begin Discharge I I
- Attain Operational Level I I I
e.
B.5.
e.
f.
If the answer to B.5.b is "Yes," briefly describe, including new maximum daily inflow rate (if applicable).9-
h.
Schedule
Implementation Stage MM/DD/YYYY
- Begin Construction //06/11/2007
- End Construction 02/06/2008 //
- Begin Discharge //I
- Attain Operational Level /I I
e.
I
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 8 of 24
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
Actual Completion
MM/DD/YYYY
Actual Completion
MM/DD/YYYY
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
applicable. For improvements planned independently of local, State, or Federal agencies,
applicable. Indicate dates as accurately as possible.
Provide dates imposed by any compliance schedule or any actual dates of completion for the implementation steps listed below, as
indicate planned or actual completion dates, as
Scheduled improvements and Schedules of Implementation. Provide information on any uncompleted implementation schedule or
uncompleted plans for improvements that will affect the wastewater treatment, effluent quality, or design capacity of the treatment works. If the
treatment works has several different implementation schedules or is planning several improvements, submit separate responses to question B.5
for each. (If none, go to question B.6.)
List the outfall number (assigned in question A.9) for each outfall that is covered by this implementation schedule.
001-Reclaimed Water System. The project includes construction of a reclaimed water pump station and transmission
force main to the Pine Hollow Golf Course. Construction should be completed by early February 2008.
Indicate whether the planned improvements or implementation schedule are required by local, State, or Federal agencies.
Yes S No
Provide dates imposed by any compliance schedule or any actual dates of completion for the implementation steps listed below, as
applicable. For improvements planned independently of local, State, or Federal agencies, indicate planned or actual completion dates, as
applicable. Indicate dates as accurately as possible.
Have appropriate penmits/clearances concerning other Federal/State requirements been obtained? 0 Yes No
Describe briefly: An Authorization to Construct Permit for these improvements was issued by NCDENR on February 17, 2005
Have appropriate permits/clearances concerning other Federal/State requirements been obtained? Yes No
Describe briefly: An Authorization to Construct Permit for these improvements was issued by NCDENR on September 20, 2006.
B.6.
Outfall Number: 001
AVERAGE DAILY DISCHARGE
POLLUTANT MUMDL
Cone.Units Cone.Units
13.98 mg/L 0.61 mg/L 417 SM4500NH3F 0.5 mg/L
N/A N/A
DISSOLVED OXYGEN 10.6 mg/L 8.29 mg/L 396
I 3.36 mg/L 1.56 mg/L 94 EPA 351.2 0.25 mg/L
17 mg/L 2.57 mg/L 94 EPA 353.2 0.02 mg/L
OIL and GREASE < 5 mg/L < 5 mg/L 3 EPA 1664 A 5 mg/L
PHOSPHORUS (Total)5.83 mg/L 1.36 mg/L 101 SM 4500 P 0.1 mg/L
565 *mg/L <1 mg/L 4 SM 2540 D 1 mg/L
OTHER
I
I
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 9 of 24
TOTAL KJELDAHL
NITROGEN (TKN)
CHLORINE (TOTAL
RESIDUAL, TRO)
NITRATE PLUS NITRITE
NITROGEN
END OF PART B.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
MAXIMUM DAILY
DISCHARGE
Number of
Samples
ANALYTICAL
METHOD
TOTAL DISSOLVED SOLIDS
(TDS) * See Note Below *
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
AMMONIA (as N)
Note: The Total Dissolved Solids sample analyzed on 9/13/07 yielded a high result of 565 mg/L. This result was suspect and an additional sample
was taken and analyzed on 9/27/07. The results of this latter test was a concentration of less than 1 mg/L. This result is consistent with previous
test results yielding 1 mg/L or less. Therefore, we believe the result of 565 mg/L to be an outlier and not reflective of the TDS concentrations
exhibited at the LCWRF.
EFFLUENT TESTING DATA (GREATER THAN 0.1 MGD ONLY).
Applicants that discharge to waters of the US must provide effluent testing data for the following parameters. Provide the indicated
effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include Information
on combine sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate
QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a minimum effluent testing data must be
based on at least three pollutant scans and must be no more than four and on-half years old.
BASIC APPLICATION INFORMATION
PARTC. CERTIFICATION
ALL APPLICANTS MUST COMPLETE THE FOLLOWING CERTIFICATION.
Name and official title R. Steven Biggs, Town Manager
Signature
Telephone number [phone]
Date signedI
works or identify appropriate permitting reguirements.
SEND COMPLETED FORMS TO:
I
27699-1617
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 10 of 24
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
NCDENR/ DWQ
Attn: NPDES Unit
1617 Mail Service Center
Raleigh, North Carolina
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
Upon reguest of the permitting authority, you must submit any other information necessary to assure wastewater treatment practices at the treatment
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system
designed to assure that gualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who
manage the system or those persons directly responsible for gathering the information, the information is, to the best of my knowledge and belief, true,
accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment
for knowing violations.
All applicants must complete the Certification Section. Refer to instructions to determine who is an officer for the purposes of this
certification. All applicants must complete all applicable sections of Form 2A, as explained In the Application Overview. Indicate below which
parts of Form 2A you have completed and are submitting. By signing this certification statement, applicants confirm that they have reviewed
Form 2A and have completed all sections that apply to the facility for which this application is submitted.
Indicate which parts of Form 2A you have completed and are submitting:
0 Basic Application Information packet Supplemental Application Information packet:
S Part D (Expanded Effluent Testing Data)
Part E (Toxicity Testing: Biomonitoring Data)
Part F (Industrial User Discharges and RCRA/CERCLA Wastes)
□ Part G (Combined Sewer Systems)
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:RIVER BASIN:I Renewal Neuse
SUPPLEMENTAL APPLICATION INFORMATION
PART D. EXPANDED EFFLUENT TESTING DATA
I Refer to the directions on the cover page to determine whether this section applies to the treatment works.
Outfall number:001
AVERAGE DAILY DISCHARGE
POLLUTANT ML/MDLCone.Units Mass Units Cone.Units Mass Units
METALS (TOTAL RECOVERABLE), CYANIDE, PHENOLS, AND HARDNESS.
ANTIMONY < 3 ug/L < 3 ug/L 3 SM 3113 B 3
ARSENIC < 5 ug/L < 5 ug/L 3 SM 3113 B 5
BERYLLIUM < 1 ug/L < 1 ug/L 3 ERA 200.7 1
CADMIUM < 1 ug/L < 1 ug/L 3 SM 3113 B 1
CHROMIUM < 5 ug/L < 5 ug/L 3 ERA 200.7 5
COPPER 16 ug/L 11 ug/L 3 ERA 200.7 2
LEAD < 5 ug/L < 5 ug/L 3 SM 3113 B 5
MERCURY 0.2 ug/L 0.14 ug/L 3 ERA 245.1 0.2
NICKEL < 10 ug/L < 10 ug/L 3 ERA 200.7 10
SELENIUM < 10 ug/L < 10 ug/L 3 SM 3113B 10
SILVER < 5 ug/L < 5 ug/L 3 ERA 200.7 5
THALLIUM < 10 ug/L < 10 ug/L 3 ERA 200.7 5
ZINC 144 ug/L 116 ug/L 3 SM 3113 B 100
CYANIDE < .005 mg/L < .005 mg/L 3 SM 4500 CN-E .005
2 ug/L < 2 ug/L 3 SM 510 A&E .01
HARDNESS (as CaCO3)73 mg/L 63 mg/L 3 SM 2340 C 2
Use this space (or a separate sheet) to provide information on other metals requested by the permit writer
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 11 of 24
Number
of
Samples
TOTAL PHENOLIC
COMPOUNDS
Little Creek Water Reclamation Facility,
NC0025453
ANALYTICAL
METHOD
(Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE
Effluent Testing: 1.0 mgd and Pretreatment Works. If the treatment works has a design flow greater than or equal to 1.0 mgd or it has (or is required
to have) a pretreatment program, or is otherwise required by the permitting authority to provide the data, then provide effluent testing data for the following
pollutants. Provide the indicated effluent testing information and any other information required by the permitting authority for each outfall through which
effluent is discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected
through analyses conducted using 40 CFR Part 136 methods. In addition, these data must comply with QA/QC requirements of 40 CFR Part 136 and
other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. Indicate in the blank rows provided below
any data you may have on pollutants not specifically listed in this form. At a minimum, effluent testing data must be based on at least three pollutant
scans and must be no more than four and one-half years old.
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:RIVER BASIN:I Renewal Neuse
Outfall number: 001I AVERAGE DAILY DISCHARGE
POLLUTANT MUMDLCone.Units Mass Units Cone.Units Mass Units
VOLATILE ORGANIC COMPOUNDS
ACROLEIN < 100 ug/L < 100I ug/L 3 EPA 624 100
ACRYLONITRILE < 100 ug/L < 100 ug/L 3 EPA 624 100
BENZENE < 5 ug/L < 5 ug/L 3 EPA 624 5
BROMOFORM < 5 ug/L < 5 ug/L 3 EPA 624 5
< 5 ug/L < 5 ug/L 3 EPA 624 5
CHLOROBENZENE < 5 ug/L < 5 ug/L 3 EPA 624 5I< 5 ug/L < 5 ug/L 3 EPA 624 5
CHLOROETHANE < 10 ug/LI < 10 ug/L 3 EPA 624 10
< 5 ug/L < 5 ug/L 3 EPA 624 5
CHLOROFORM < 5 ug/L < 5 ug/L 3 EPA 624 5
< 5 ug/L < 5 ug/L 3 EPA 624 5
1,1-DICHLOROETHANE < 5 ug/L < 5 ug/L 3 EPA 624 5
1,2-DICHLOROETHANE < 5 ug/L < 5 ug/L 3 EPA 624 5
< 5 ug/L < 5 ug/L 3 EPA 624 5
I < 5 ug/L < 5 ug/L 3 EPA 624 5
1,2-DICHLOROPROPANE < 5 ug/L < 5 ug/L 3 EPA 624 5
< 5 ug/L < 5 ug/L 3 EPA 624 5
ETHYLBENZENE < 5 ug/L < 5 ug/L 3 EPA 624 5
METHYL BROMIDE < 10 ug/L < 10 ug/L 3 EPA 624 10
METHYL CHLORIDE < 10 ug/L < 10 ug/L 3 EPA 624 10
METHYLENE CHLORIDE < 10 ug/L < 10 ug/L 3 EPA 624 10
< 5 ug/L < 5 ug/L 3 EPA 624 5
< 5 ug/L < 5 ug/L 3 EPA 624 5
TOLUENE < 5 ug/L < 5 ug/L 3 EPA 624 5
I EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 12 of 24
Number
of
Samples
CARBON
TETRACHLORIDE
DICHLOROBROMO­
METHANE
1,1,2,2-TETRA-
CHLOROETHANE
Little Creek Water Reclamation Facility,
NC0025453
ANALYTICAL
METHOD
CHLORODIBROMO­
METHANE
2-CHLOROETHYLVINYL
ETHER
TRANS-1.2-DICHLORO-
ETHYLENE
1,1-DICHLORO-
ETHYLENE
1,3-DICHLORO-
PROPYLENE
TETRACHLORO­
ETHYLENE
(Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:RIVER BASIN:I Renewal Neuse
Outfall number: 001
MAXIMUM DAILY DISCHARGE
POLLUTANT MUMDLCone.Units Mass Units Cone.Units Mass Units
< 5 ug/L < S ug/L 3 EPA 624 5
< 5 ug/L < 5 ug/L 3 EPA 624 5
TRICHLOROETHYLENE < 5 ug/L < 5 ug/L 3 EPA 624 5
VINYL CHLORIDE < 10 ug/L <10 ug/L 3 EPA 624 10
Use this space (or a separate sheet) to provide information on other volatile organic compounds requested by the permit writer
I
ACID-EXTRACTABLE COMPOUNDS
I P-CHLORO-M-CRESOL < 20 ug/L <20 ug/L 3 EPA 625 20
2-CHLOROPHENOL < 10 ug/L < 10 ug/L 3 EPA 625 10
2,4-DICHLOROPHENOL < 10 ug/L < 10 ug/L 3 EPA 625 10
2,4-DIMETHYLPHENOL < 10 ug/L < 10 ug/L 3 EPA 625 10
4,6-DINITRO-O-CRESOL < 50 ug/L < 50 ug/L 3 EPA 625 50
2,4-DINITROPHENOL < 50 ug/L < 50 ug/L 3 EPA 625 50
2-NITROPHENOL < 10 ug/L < 10 ug/L 3 EPA 625 10
4-NITROPHENOL < 50 ug/L < 50 ug/L 3 EPA 625 50
PENTACHLOROPHENOL < 50 ug/L < 50 ug/L 3 EPA 625 50
PHENOL < 10 ug/L < 10 ug/L 3 EPA 625 10
< 10 ug/L < 10 ug/L 3 EPA 625 10IUse this space (or a separate sheet) to provide information on other acid-extractable compounds requested by the permit writer
I BASE-NEUTRAL COMPOUNDS
ACENAPHTHENE < 10 ug/L < 10 ug/L 3 EPA 625 10
ACENAPHTHYLENE < 10 ug/L < 10 ug/L 3 EPA 625 10
ANTHRACENE < 10 ug/L < 10 ug/L 3 EPA 625 10
I BENZIDINE < 100 ug/L < 100 ug/L 3 EPA 625 100
BENZO(A)ANTHRACENE < 10 ug/L < 10 ug/L 3 EPA 625 10IBENZO(A)PYRENE < 10 ug/L < 10 ug/L 3 EPA 625 10
I EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 13 of 24
Number
of
Samples
Little Creek Water Reclamation Facility,
NC0025453
ANALYTICAL
METHOD
2,4,6-
TRICHLOROPHENOL
1,1,1-
TRICHLOROETHANE
1.1,2-
TRICHLOROETHANE
(Complete once for each outfall discharging effluent to waters of the United States.)
AVERAGE DAILY DISCHARGE
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:RIVER BASIN:
Renewal Neuse
I Outfall number: 001
MAXIMUM DAILY DISCHARGE
POLLUTANT ML/MDLCone.Units Mass Units Cone.Units Mass Units
< 10 ug/L < 10 ug/L 3 ERA 625 10
BENZO(GHI)PERYLENE < 10 ug/L < 10 ug/L 3 ERA 625 10
< 10 ug/l < 10 ug/L 3 ERA 625 10
< 10 ug/L < 10 ug/L 3 ERA 625 10
I < 10 ug/L < 10 ug/L 3 ERA 625 10
< 10 ug/l < 10 ug/L 3 ERA 625 10
I < 20 ug/L < 20 ug/L 3 ERA 625 20
< 10 ug/L < 10 ug/L 3 ERA 625 10I< 10 ug/L < 10 ug/L 3 ERA 625 10
I < 10 ug/L < 10 ug/L 3 ERA 625 10
< 10 ug/L < 10 ug/L 3 ERA 625 10
CHRYSENE < 10 ug/L < 10 ug/L 3 ERA 625 10
DI-N-BUTYL PHTHALATE < 10 ug/l < 10 ug/l 3 ERA 625 10
DI-N-OCTYL PHTHALATE < 10 ug/l < 10 ug/L 3 ERA 625 10
< 10 ug/l < 10 ug/l 3 ERA 625 10
1,2-DICHLOROBENZENE < 10 ug/l < 10 ug/L 3 ERA 625 10
1,3-DICHLOROBENZENE < 10 ug/L < 10 ug/L 3 ERA 625 10
1.4-DICHLOROBENZENE < 10 ug/l < 10 ug/l 3 ERA 625 10
I < 10 ug/L < 10 ug/l 3 ERA 625 10
DIETHYL PHTHALATE < 10 ug/l < 10 ug/L 3 EPA 625 10
DIMETHYL PHTHALATE < 10 ug/l < 10 ug/l 3 EPA 625 10
2,4-DINITROTOLUENE < 10 ug/L < 10 ug/l 3 EPA 625 10
2,6-DINITROTOLUENE < 10 ug/l < 10 ug/L 3 EPA 625 10
I < 10 ug/L < 10 ug/L 3 EPA 625 10
I EPA Form 3510-2A (Rev 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 14 of 24
Number
of
Samples
Little Creek Water Reclamation Facility,
NC0025453
BENZO(K)
FLUORANTHENE
BIS (2-CHLOROETHOXY)
METHANE
2-CHLORO-
NAPHTHALENE
1,2-DIPHENYL-
HYDRAZINE
ANALYTICAL
METHOD
3,4 BENZO­
FLUORANTHENE
BIS (2-CHLOROETHYL)-
ETHER
BIS (2-CHLOROISO-
PROPYL) ETHER
4-BROMOPHENYL
PHENYL ETHER
4-CHLORPHENYL
PHENYL ETHER
DIBENZO(A.H)
ANTHRACENE
BIS (2-ETHYLHEXYL)
PHTHALATE
BUTYL BENZYL
PHTHALATE
3,3-DICHLORO-
BENZIDINE
(Complete once for each outfall discharging effluent to waters of the United States.)
AVERAGE DAILY DISCHARGE
FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:RIVER BASIN:
Renewal Neuse
Outfall number: 001
AVERAGE DAILY DISCHARGE
POLLUTANT ML/MDLCone.Units Mass Units Cone.Units Mass Units
FLUORANTHENE < 10 ug/L < 10 ug/L 3 EPA 625 10
FLUORENE < 10 ug/L < 10 ug/L 3 EPA 625 10
HEXACHLOROBENZENE < 10 ug/L < 10 ug/L 3 EPA 625 10
I < 10 ug/L < 10 ug/L 3 EPA 625 10
< 10 ug/L < 10 ug/L 3 EPA 625 10
HEXACHLOROETHANE < 10 ug/L < 10 ug/L 3 EPA 625 10
< 10 ug/L < 10 ug/L 3 EPA 625 10
ISOPHORONE < 10 ug/L < 10 ug/L 3 EPA 625 10
NAPHTHALENE < 10 ug/L < 10 ug/L 3 EPA 625 10
NITROBENZENE < 10 ug/L < 10 ug/L 3 EPA 625 10
I < 10 ug/L < 10 ug/L 3 EPA 625 10
< 10 ug/L < 10 ug/L 3 EPA 625 10
< 10 ug/L < 10 ug/L 3 EPA 625 10
PHENANTHRENE < 10 ug/L < 10 ug/L 3 EPA 625 10
PYRENE < 10 ug/L < 10 ug/L 3 EPA 625 10
< 10 ug/L < 10 ug/L 3 EPA 625 10
Use this space (or a separate sheet) to provide information on other base-neutral compounds requested by the permit writer
I
Use this space (or a separate sheet) to provide information on other pollutants (e g., pesticides) requested by the permit writer
I
I
I
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 15 of 24
END OF PART D.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
Number
of
Samples
Little Creek Water Reclamation Facility,
NC0025453
ANALYTICAL
METHOD
HEXACHLORO­
BUTADIENE
N-NITROSODI-
METHYLAMINE
HEXACHLOROCYCLO-
PENTADIENE
INDENO(1,2,3-CD)
PYRENE
N-NITROSODI-N-
PROPYLAMINE
N-NITROSODI-
PHENYLAMINE
1.2,4-
TRICHLOROBENZENE
(Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE
RIVER BASIN:
Neuse
SUPPLEMENTAL APPLICATION INFORMATION
PART E. TOXICITY TESTING DATA
I
I complete.
E.1.Required Tests.
acute Clayton submits quarterly toxicity testing results to NCDENR as required by their NPDES permit
E.2.Allow one
Test number: Test number: Test number:
I Test information.** SEE ATTACHED TOXICITY RESULTS **a.
Test Species & test method number
Age at initiation of test
Outfall number
Dates sample collected
Date test started
Duration
b.Give toxicity test methods followed.
Manual title
Edition number and year of publication
I Page number(s)
Give the sample collection method(s) used. For multiple grab samples, indicate the number of grab samples used.c.
I 24-Hour composite
Grab
I d.Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection
After dechlorination
EPA Form 3510-2A (Rev 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 16 of 24
PERMIT ACTION REQUESTED:
Renewal
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility, NC0025453
Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
£3 chronic
Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years,
column per test (where each species constitutes a test). Copy this page if more than three tests are being reported.
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility’s discharge points: 1) POTWs with a design flow rate greater than or equal to 1.0 mgd; 2) POTWs with a pretreatment program (or those that are
required to have one under 40 CFR Part 403); or 3) POTWs required by the permitting authority to submit data for these parameters.
• At a minimum, these results must include quarterly testing for a 12-month period within the past 1 year using multiple species (minimum of two
species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results
show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation, if one was conducted.
• If you have already submitted any of the information requested in Part E. you need not submit it again. Rather, provide the information
requested in question E.4 for previously submitted information. If EPA methods were not used, report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E.
If no biomonitoring data is required, do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
I
RIVER BASIN:
Neuse
Test number:Test number:Test number:
Describe the point in the treatment process at which the sample was collected.e.
Sample was collected:
f.For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both
Chronic toxicity
Acute toxicity
g. Provide the type of test performed.
I Static
Static-renewal
Flow-through
h.
Laboratory water
Receiving water
Type of dilution water. If salt water, specify “natural" or type of artificial sea salts or brine used.
Fresh water
Salt water
j-Give the percentage effluent used for all concentrations in the test series.
I
k.Parameters measured during the test. (State whether parameter meets test method specifications)
I pH
Salinity
I Temperature
Ammonia
I Dissolved oxygen
I.Test Results.
I Acute:
%%%
LCso
95% C.l.%%%
Control percent survival %%%
Other (describe)
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 17 of 24
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility, NC0025453
Percent survival in 100%
effluent
PERMIT ACTION REQUESTED:
Renewal
Source of dilution water. If laboratory water, specify type; if receiving water, specify source.
Chronic:
NOEC %%%
IC25 %%%
Control percent survival %%%
Other (describe)
m. Quality Control/Quality Assurance.
Is reference toxicant data available?
I
////I I
Other (describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
□ Yes 0 No If yes. describe:
I
E.4. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information, or information regarding the
L IDate submitted:(MM/DD/YYYY)
Summary of results: (see instructions)
The Town of Clayton submits quarterly chronic toxicity tests to NCDENR in accordance with their NPDES permit
Please see attached summary of the most recent test results.
I
I
I
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 18 of 24
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility, NC0025453
Was reference toxicant test within
acceptable bounds?
END OF PART E.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
RIVER BASIN:
Neuse
What date was reference toxicant test
run (MM/DD/YYYY)?
PERMIT ACTION REQUESTED:
Renewal
cause of toxicity, within the past four and one-half years, provide the dates the information was submitted to the permitting authority and a summary
of the results.
SUPPLEMENTAL APPLICATION INFORMATION
PART F. INDUSTRIAL USER DISCHARGES AND RCRA/CERCLA WASTES
GENERAL INFORMATION:
F.1.
F.2.
Number of non-categorical Sills.a.1
b.Number of CIUs.3
SIGNIFICANT INDUSTRIAL USER INFORMATION:
F.3.Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
Name:Talecris Biotherapeutics, Inc.
Mailing Address:8368 US Highway 70 West
Clayton, NC 27520
F.4.
Extraction Machines
F.5.
Principal product(s):Biological Medical Products
I Raw material(s):Blood Plasma
F.6.Flow Rate.
a.
300,000 gpd (XX continuous or
b.
100,000 gpd (XX continuous or intermittent)I F.7.Pretreatment Standards. Indicate whether the SIU is subject to the following:
Local limits 13 Yes Noa.
b.Categorical pretreatment standards 13 Yes No
If subject to categorical pretreatment standards, which category and subcategory?
CFR 439.20
ERA Form 3510-2A (Rev 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 19 of 24
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
Pretreatment program. Does the treatment works have, or is subject to, an approved pretreatment program?
0 Yes No
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
one SIU discharges to the treatment works, copy questions F.3 through F.8 andSupply the following information for each SIU. If more than
provide the information requested for each SIU.
Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works.
intermittent)
Non-process wastewater flow rate. Indicate the average daily volume of non-process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent.
All treatment works receiving discharges from significant industrial users or which receive RCRA.CERCLA, or other remedial wastes must
complete part F.
Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent.
F.8.
Amount Units
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to origniate in
the next five years).
F.15. Waste Treatment
a.
I If yes, describe the treatment (provide information about the removal efficiency):
b.
Intermittent If intermittent, describe discharge schedule.
I
EPA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 20 of 24
END OF PART F.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
Is the discharge (or will the discharge be) continuous or intermittent?
Continuous
Is this waste treated (or will be treated) prior to entering the treatment works?
Yes No
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
F.12. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
Yes (complete F.13 through F.15.) No
Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe?
Yes No (go to F.12)
Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e g.,
upsets, interference) at the treatment works in the past three years?
Yes 0 No If yes, describe each episode.
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE:
F.9. RCRA Waste.
CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
F.10. Waste transport Method by which RCRA waste is received (check all that apply):
Truck Rail Dedicated Pipe
F.11. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units).
EPA Hazardous Waste Number
F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received). Include data on volume and concentration, if
known. (Attach additional sheets if necessary.)
RIVER BASIN:
Neuse
SUPPLEMENTAL APPLICATION INFORMATION
PART F. INDUSTRIAL USER DISCHARGES AND RCRA/CERCLA WASTES
GENERAL INFORMATION:
F.1.
F.2.
Number of non-categorical SIUs.c.1
d.Number of CIUs.3
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU. If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU.
Name:Novo Nordisk Pharmaceutical Industries. Inc.
Mailing Address:3612 Powhattan Road
Clayton, NC 27520
F.4.Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU’s discharge.I Mixing and Packaging
F.5.
Principal product(s):Insulin for I.V, Injection
I Raw material(s):Insulin Crystals
F.6.Flow Rate.
c.
90.500 gpd (XX continuous or intermittent)
d.
34,200 (XXgpd continuous or intermittent)
F.7.Pretreatment Standards. Indicate whether the SIU is subject to the following:
Local limits C3 Yes Noa.
I Categorical pretreatment standards El Yesb. No
If subject to categorical pretreatment standards, which category and subcategory?
CFR 439.40
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 21 of 24
Non-process wastewater flow rate. Indicate the average daily volume of non-process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent.
PERMIT ACTION REQUESTED:
Renewal
All treatment works receiving discharges from significant industrial users or which receive RCRA.CERCLA, or other remedial wastes must
complete part F.
Pretreatment program. Does the treatment works have, or is subject to, an approved pretreatment program?
E3 Yes No
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU s
discharge.
Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent.
Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works.
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
I
F.8.
Amount Units
F.12. Remediation Waste.
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to origniate in
the next five years).
F.15. Waste Treatment
c.
If yes, describe the treatment (provide information about the removal efficiency):
d.
Intermittent If intermittent, describe discharge schedule.I
ERA Form 3510-2A (Rev. 1-99). Replaces ERA forms 7550-6 & 7550-22.Page 22 of 24
END OF PART F.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
Yes (complete F.13 through F.15.) No
Is this waste treated (or will be treated) prior to entering the treatment works?
Yes No
Is the discharge (or will the discharge be) continuous or intermittent?
Continuous
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e g.,
upsets, interference) at the treatment works in the past three years?
Yes 0 No If yes, describe each episode.
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE:
F.9. RCRA Waste. Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe?
Yes 0 No (go to F.12)
CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
F.10. Waste transport. Method by which RCRA waste is received (check all that apply):
Truck Rail Dedicated Pipe
F.11. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units).
EPA Hazardous Waste Number
F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received). Include data on volume and concentration, if
known. (Attach additional sheets if necessary.)
RIVER BASIN:
Neuse
SUPPLEMENTAL APPLICATION INFORMATION
PART F. INDUSTRIAL USER DISCHARGES AND RCRA/CERCLA WASTES
GENERAL INFORMATION:
F.1.
F.2.
Number of non-categorical SIUs.1e.
f.Number of CIUs.3
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU. If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU.
F.3.
Name:Hospira, Inc.
Mailing Address:8484 U.S 70 West
Clayton, NC 27520
F.4.Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU’s discharge.
Mixing and Packaging
F.5.
Principal product(s):I.V. Nutritional Products
Raw material(s):Soybean Oil, Egg Yolks. Glycerin, Amino Acids
F.6.Flow Rate.
e.
65.000 gpd (XX continuous or
f.
35,000 gpd (XX continuous or intermittent)
F.7.Pretreatment Standards. Indicate whether the SIU is subject to the following:
Local limits Yes Noa.
Categorical pretreatment standards 13 Yesb. No
If subject to categorical pretreatment standards, which category and subcategory?
CFR 439.40
ERA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 23 of 24
Pretreatment program. Does the treatment works have, or is subject to, an approved pretreatment program?
E Yes No
PERMIT ACTION REQUESTED:
Renewal
All treatment works receiving discharges from significant industrial users or which receive RCRA.CERCLA, or other remedial wastes must
complete part F.
Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day (gpd) and whether the discharge is continuous or intermittent.
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
intermittent)
Non-process wastewater flow rate. Indicate the average daily volume of non-process wastewater flow discharged into the collection system
in gallons per day (gpd) and whether the discharge is continuous or intermittent.
Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works.
F.8.
RCRA Waste.
Amount Units
I
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to origniate in
the next five years).I
I F.15. Waste Treatment
e.
If yes, describe the treatment (provide information about the removal efficiency):
f.
Intermittent If intermittent, describe discharge schedule.
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22.Page 24 of 24
END OF PART F.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
Is the discharge (or will the discharge be) continuous or intermittent?
Continuous
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
Is this waste treated (or will be treated) prior to entering the treatment works?
Yes No
FACILITY NAME AND PERMIT NUMBER:
Little Creek Water Reclamation Facility,
NC0025453
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE:
F.9.
F.12. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
Yes (complete F.13 through F.15.) 0 No
Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e g
upsets, interference) at the treatment works in the past three years?
Yes 0 No If yes, describe each episode.
CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe?
Yes 0 No (go to F.12)
F.10. Waste transport Method by which RCRA waste is received (check all that apply):
Truck Rail Dedicated Pipe
F.11. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units).
EPA Hazardous Waste Number
F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received). Include data on volume and concentration, if
known. (Attach additional sheets if necessary.)
I
I
I
I
Topographic Map
Schematic Flow Diagram
Process Flow Diagram
I
Priority Pollutant Analyses (PPAs)
I
<5
Cyanide
Grab
<5
Grab
Grab
Grab
Page 1
<100|ug/l.crolein
.crylonitrile
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I
I
I
I
_1
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2_
1
1
7
i
■'halljiirn
Zinc
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Silver
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Lead
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Composite
Composite
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Composite
Composite
Composite
Composite
Composite
Composite
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EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 200.7
EPA 200.7
EPA 200.7
EPA 200.7
EPA 200.7
EPA 200.7
EPA 200.7
EPA 245.1
EPA 200.7
EPA 200.7
EPA 200.7
SM3113B
EPA 200.7
EPA 335.3
100
100
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5
6
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10
5
5
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5
1
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1
lercury
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____!_
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0.25__
0.1 ___
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0.1 _
5 <5
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110
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ug/1
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ug/!
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ug/1
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ug/1
ug/1
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3.2
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<.02
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Composite
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7/28/05 Grab
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EPA 353.2
EPA 351,2
SM 4500P-E
SM 2540D
EPA 200.7
SM4500-C1 D
EPA1664A
.6Z7;05 U8/,
£
£
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£
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£
£
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£
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1
mg/1
mg/l
mg/1
mg/l
mg/l
mg/l
mg/l
ug/1
mg/l
Antimony
Wusenic
^yeryIlium
Cadmium
■fchromium
Ug/1
<5 ug/1
<10 ug/1
<1^| ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
<51 ug/1
ug/1
ug/1
ug/1
_________________i— <.0051 mg/l
btal phenolic compounds_____________Grab EPA 420.1 •< O( <^--01 mg/l 1
£
£
£
£
£
£
£
1
^Benzene
Bromoform
y arbon tetrachloride_____
<1 hloro benzene
jDhlorodibromoniethane
jlbloroe thane
™ -chloroethylvinyl ether
Chloroform_______
■iiehlorobi-omomethane___
W, 1 -dichloroethane
1,2 -dichloroethane
Jrans-1,2-dichloroethylene
ORC James Warren
.Phone [phone]
Town of Clayton Little Creek Water Reclamation Facility
| Ammonia (as N) 6/15/05
pissolved oxygen 6/15/05
f'Jitrate/Nitrite 6/15/05
Total Kjeldahl nitrogen 6/15/05
Jotal Phosphorus 6/15/05
fotal dissolved solids
Hardness 6/7/05
Chlorine (total residual, TRC)
|3il and grease 6/7/05
" Town Of Clayton
Facility Name Little Creek Water Reclamation Facility
■Date of sampling 6/7/05, 6/15/05
Analytical Laboratory Pace Anvltical, Tri Test.
I
I
ttg/l
lane
I ug/1
I
I
I
Page 2
<5
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<50
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EPA 625
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__EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
__EPA 625
__ EPA 625
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EPA 625
EPA 625
' EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
<5 ug/I
ug/1
ug/1
ug/1
ug/1
<51 ug/1
ug/1
ug/1
ug/1
ug/1
<8
<8
<10 ug/1
<10
<5 ug/1
<7 ug/1
<5
<61 ug/1
<5 ug/1
<5 ug/1
<5 ug/1
<5 ug/1
<5 ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
<5 ug/1
<61 ug/1
ug/1
ug/1
Acenaphthene
Acenaphthylene
Anthracene
Benzidine
Benzo(a)anthracene
Benzo(a)pyrene ~ "
3,4 benzofluoranthene
Benzo(ghi)perylene
Benzo (k)fluoranthene
Bis (2-chloroethoxy) methane
Bis (2-chloroethyl) ether
Bis (2-chloroisopropyl) ether
Bis (2-ethylhexyl) phthalate
4-bromophenyl phenyl ether
Butyl benzyl phthalate
2 -chloronaphthalene
4-chIorophenyl phenyl ether
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
2 -chlorophenol
2,4 -dichlorophenol
2,4 -dimethylphenol
4,6-dinitro-o-cresol
2,4-dinitrophenol
2-nitrophenol
4-nitrophenol
Pentachlorophenol
Phenol
2,4,6 -trichlorophenol
Grab
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<5
<25
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ug/1
ug/1
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ug/1
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11
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Z3 3 3 3 3 3 3_ jj 3 3 3
—
Punster
VoUtilvorgaHid___
1.1 -dichloroethylene
1.2 -dichloropropane
1,3-dichloropropylene
Ethylbenzene
Methyl bromide
Methyl chloride
Methylene chloride
1.1.2.2- tetrachloroeth;
Tetrachloroethylene
Toluene
1,1,1 -trichloroethane
1.1.2- trichloroethane
Trichloroethylene
Vinyl chloride
............... Grab
2-chloroDhenol --------------------------El. ---------------------------^1________^Pg/1
5
5
__5
25
25
5
25
25
5
________________ 5
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5
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___ I
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____________(san _
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Date
Page3
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EPA 625
~ EPA 625
~ EPA 625
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~ EPA 625
' EPA 625
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5
5,
5
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22
5 "
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5 '
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ug/l
ug/1
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ug/1
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ug/1
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ug/1
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ug/1
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Authorized Representative name
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<51 ug/1
ug/1
<51 ug/1
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ug/1
ug/1
ug/1
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<10
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prepared under my director
C^nature
I05-
1 ■ I
H
1
1
'_1
__1
1
1
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1
1
1
Chiysene
Di-n-butyl phthalate
Di-n-octyl phthalate
Dibenzo(a,h)anthracene
1.2- dichlorobenzene
1.3- dichlorobenzene
1.4- dichlorobenzene
3.3- dichloro benzidine
Diethyl phthalate
Dimethyl phthalate
2.4- dinitrotoluene
2,6-dinitrotoluene
1,2-diphenylhydrazine
Fluoranthene
Fluorene
Hexachlorobenzene
Hexachlorobutadiene
Hexachlorocyclo-pentadiene
Hexachloroethane
Indeno( 1,2,3-cd)pyrene
Isophorone
N aphthalene
Nitrobenzene
N -nitrosodi-n-propylamine
N -nitrosodimethylamine
N-nitrosodiphenylamine
Phenanthrene
Pyrene
1 >2,4,-trichlorobenzene
I certify under penalty of law that this document and all attachments were, ' -
and supervtsion in accordance with a system to design to assure that qualified perdonnel pro^periv '
gather and evaluat the information submitted. Based on my inquiry of the person or persons toat
manage the system, or those persons directly responsibel for gathering the information the
aw^amernT^- “ 1116 “curate and complete. I am
Tr penalties for submitting false information, including the
possibility of fines and imprisonment for knowing violations.
s ------
June 23. 2005
1
RE:
Dear Mr. Warren:
If you have any questions concerning this report please feel free to contact me.
Sincerely.
Enclosures
I
I
Annette Scott
Annette.ScottPpacelabs.com
Project Manager
Lab Project Number:
Client Project ID:
40
37712
99030
E87648
9296272
PPO 6/7
Mr. James Warren
Town of Clayton
P.O. Box 879
1000 DURHAM ST
Clayton. NC 27520
Pace Analytical Services, Inc.
9800 Kincey Avenue, Suite 100
Huntersville, NC 28078
Phone: [phone]
Fax: [phone]
Pace Analytical Services, Inc.
2225 Riverside Drive
Asheville, NC 28804
Phone: [phone]
Fax: [phone]
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services. Inc
/HPhr-
Ashville Certification IDs
NC Wastewater
NC Drinking Water
SC Environment
FL NELAP
Charlotte Certification IDs
NC Waste water 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627
ace Analytical
www.pacelabs.com
Inorganic Wet Chemistry and Metals Analyses were performed at our Pace Asheville laboratory and Organic testing
was performed at our Pace Charlotte laboratory unless otherwise footnoted.
Enclosed are the analytical results for sample(s) received by the laboratory on June 9. 2005. Results reported
herein conform to the most current NELAC standards, where applicable, unless otherwise narrated in the body of
the report.
I
I
Results Units Report Limit Analyzed By CAS No.
7440-66-6
0.00020 06/10/05 04:41 ALV 7439-97-6
0.010 06/14/05 14:44 SHE
I
Date: 06/23/05
Page: 1 of 6
I
Mercury, CVAAS, in Water
Mercury
40
37712
99030
E87648
Method: ERA 420.1
ND mg/1
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
0.0050
0.0050
0.0010
0.0010
0.0020
0.0020
0.0050
0.0050
0.0050
0.0020
2.0
0.010
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
83-32-9
208-96-8
120-12-7
92-87-5
207-08-9
205-99-2
56-55-3
191-24-2
50-32-8
101-55-3
85-68-7
59-50-7
111-91-1
111-44-4
7440-36-0
7440-38-2
7440-41-7
7440-43-9
7440-47-3
7440-50-8
7439- 92-1
7440- 02-0
7782-49-2
7440-22-4
Date Collected: 06/07/05 09:15
Date Received: 06/09/05 16:00
GC/MS Semi vol atlies
Extractables in Water by 625
Acenaphthene
Acenaphthylene
Anthracene
Benzidine
Benzo(k)fluoranthene
Benzo(b)flucranthene
Benzo(a)anthracene
Benzo(g.h.i)perylene
Benzo(a)pyrene
4-Bromophenylphenyl ether
Butyl benzyl phthalate
4-Chioro-3-methyl phenol
bis(2-Chloroethoxy)methane
bis(2-Chloroethyl) ether
Method: ERA 245.1
ND mg/1
Prep/Method:
ND
ND
ND
ND
0.0032
ND
ND
ND
ND
ND
73.
0.11
06/10/05 01:45
5.0
5.0
5.0
50.
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
Pace Analytical Services. Inc.
2225 Riverside Drive
Asheville, NG 28804
Phone: [phone]
Fax: [phone]
Wet Chemistry
Phenolics. Total Recoverable
Phenolics. Total Recoverable
Pace Analytical Services, Inc.
9800 Kincey Avenue, Suite 100
Huntersville, NC 28078
Phone: [phone]
Fax: [phone]
Lab Project Number: 9296272
Client Project ID: PPO 6/7
Parameters_____
Metals
Trace ICP Metals
Antimony
Arsenic
Beryllium
Cadmium
Chromium
Copper
Lead
Nickel
Selenium
Silver
Total Hardness
Zinc
Date Digested
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
.. '* *CCo^
nptar-
MAshville Certification IDs
|NC Waste water
NC Drinking Water
SC Environment
■fl nelap
Project Sample Number: 9296272-001
Matrix: Water
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/15/05 06:18 ALV
06/10/05 01:45
/ EPA 200.7
mg/1
mg/1
mg/1
mg/1
mg/1
mg/1
mg/1
mg/1
mg/1
mg/1
mg/1
mg/1
race Analytical®
www.pacelabs.com
Charlotte Certification IDs
NC Wastewater 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627
Prep/Method: EPA 625 SF / EPA 625
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
Lab Sample No: 925731812
Client Sample ID: EFFLUENT COMP
I
By
I
I
Date: 06/23/05
Page: 2 of 6
Results
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
11.
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
91-20-3
98-95-3
88-75-5
100-02-7
62-75-9
621-64-7
86-30-6
Date Collected: 06/07/05 09:15
Date Received: 06/09/05 16:00
Pace Analytical Services, Inc.
2225 Riverside Drive
Asheville, NC 28804
Phone: [phone]
Fax: [phone]
JMC Wastewater
NC Drinking Water
SC Environment
■=1 NELAP
Parameters_________________
bis(2-Chloroisopropyl) ether
2-Chloronaphthalene
2-Chlorophenol
4-Chlorophenyl phenyl ether
Chrysene
Dibenz(a.h)anthracene
1.2- Di chlorobenzene
1.3- Dichlorobenzene
1.4- Dichlorobenzene
3,3'-Di chlorobenzidine
2.4- Dichlorophenol
Diethyl phthal ate
2.4- Di methyl phenol
Dimethylphthalate
Di-n-butylphthalate
4.6 - D i ni t ro- 2 - methyl phenol
2.4- Dinitrophenol
2.4- Dinitrotoluene
2,6-Dinitrotoluene
Di-n-octyl phthal ate
1.2 - Di phenylhydrazi ne
bi s(2 - Ethyl hexyl)phthalate
Fluoranthene
Fluorene
Hexachloro-1.3-butadiene
Hexachlorobenzene
Hexachlorocyclopentadiene
Hexachloroethane
Indeno(l,2.3-cd)pyrene
Isophorone
2-Methylphenol (o-Cresol)
3&4-Methyl phenol
Naphthalene
Nitrobenzene
2-Nitrophenol
4-Nitrophenol
N-Nitrosodi methyl amine
N-Nitroso-di-n-propyl amine
N•Ni trosodi phenyl amine
Units
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
Report Limit
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
10.
5.0
5.0
5.0
5.0
5.0
25.
25.
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
5.0
10.
5.0
5.0
5.0
5.0
10.
5.0
5.0
5.0
25.
5.0
5.0
5.0
CAS No.
39638-32-9
91-58-7
95-57-8
7005-72-3
218-01-9
53-70-3
95-50-1
541-73-1
106-46-7
91-94-1
120- 83-2
84-66-2
105-67-9
131-11-3
84-74-2
534-52-1
51-28-5
121- 14-2
606-20-2
117-84-0
122- 66-7
117- 81-7
206-44-0
86- 73-7
87- 68-3
118- 74-1
77- 47-4
67-72-1
193-39-5
78- 59-1
95-48-7
Project Sample Number: 9296272-001
Matrix: Water
Analyzed
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
.shville Certification IDs
40
37712
99030
E87648
race Analytical®
www.pacelabs.com
Charlotte Certification IDs
NC Wastewater 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627
Pace Analytical Services, Inc.
9800 Kincey Avenue. Suite 100
Huntersville, NC 28078
Phone: [phone]
Fax: [phone]
Lab Project Number: 9296272
Client Project ID: PPO 6/7
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services. Inc.
/ I
Lab Sample No: 925731812
Client Sample ID: EFFLUENT COMP
By Qual RegLmt
I
Date: 06/23/05
Page: 3 of 6
Date Collected: 06/07/05 09:15
Date Received: 06/09/05 16:00
Pace Analytical Services, Inc.
2225 Riverside Drive
Asheville, NC 28804
Phone: [phone]
Fax: [phone]
Units
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
*
I
Pace Analytical Services, Inc.
9800 Kincey Avenue, Suite 100
Huntersville, NC 28078
Phone: [phone]
Fax: [phone]
Lab Project Number: 9296272
Client Project ID: PPO 6/7
Parameters___________
Pentachlorophenol
Phenanthrene
Phenol
Pyrene
1.2,4-Tri chiorobenzene
2.4.6- Tri chlorophenol
Nitrobenzene-d5 (S)
2-Fluorobi phenyl (S)
Terphenyl-dl4 (S)
Phenol-d5 (S)
2-Fluorophenol (S)
2.4.6- Tribromophenol (S)
Date Extracted
CAS No.
87- 86-5
85-01-8
108-95-2
129-00-0
120-82-1
88- 06-2
4165-60-0
321-60-8
1718-51-0
4165-62-2
367-12-4
Results
ND
ND
ND
ND
ND
ND
54
56
77
23
33
80
06/14/05
Report Limit
25.
5.0
5.0
5.0
5.0
5.0
Project Sample Number: 9296272-001
Matrix: Water
■NC Wastewater
"NC Drinking Water
SC Environment
|FL NELAP
■shville Certification IDs
40
37712
99030
E87648
race Analytical®
www.pacelabs.com
Charlotte Certification IDs
NC Waste water 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
/npbX
Analyzed
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/16/05 07:01 BET
06/14/05
Lab Sample No: 925731812
Client Sample ID: EFFLUENT COMP
Results Units Report Limit Analyzed By CAS No.lual RegLmt
0.0050 06/11/05 08:45 ARH 57-12-5
5.0 06/20/05 15:19 SMI
I
Date: 06/23/05
Page: 4 of 6
40
37712
99030
E87648
100
100
5.0
5.0
5.0
10.
5.0
6.0
10.
10.
5.0
10.
5.0
5.0
5.0
5.0
5.0
6.0
5.0
5.0
8.0
5.0
7.0
5.0
6.0
5.0
5.0
5.0
10.
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
107-02-8
107- 13-1
71-43-2
75-27-4
75-25-2
74- 83-9
56-23-5
108- 90-7
75- 00-3
110-75-8
67-66-3
74- 87-3
124-48-1
75- 34-3
107- 06-2
75-35-4
156-60-5
78- 87-5
10061-01-5
10061-02-6
100-41-4
75-09-2
79- 34-5
127-18-4
108- 88-3
71-55-6
79-00-5
79-01-6
75-69-4
Date Collected: 06/07/05 14:00
Date Received: 06/09/05 16:00
GC/MS Volatiles
Volatile GC/MS by 624
Acrolein
Acrylonitrile
Benzene
Bromodi chioromethane
Bromoform
Bromomethane
Carbon tetrachloride
Chlorobenzene
Chloroethane
2-Chloroethylvinyl ether
Chloroform
Chloromethane
Di bromochioromethane
1.1- Dichloroethane
1.2- Dichloroethane
1.1- Dichloroethene
trans-1.2-Di chloroethene
1.2- Dichloropropane
cis -1.3-Di chloropropene
trans -1.3 - Di chioropropene
Ethyl benzene
Methylene chloride
1.1.2.2- Tetrachloroethane
Tetrachloroethene
Toluene
1.1.1- Tri chioroethane
1.1.2- Tri chioroethane
Tri chloroethene
Tri chlorofluoromethane
GC Semi vol atlies
Hexane Extractable Material
Oil and Grease
Method:
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
ND
Method:
ND
Pace Analytical Services, Inc.
9800 Kincey Avenue, Suite 100
Huntersville, NC 28078
Phone: [phone]
Fax: [phone]
Lab Project Number: 9296272
Client Project ID: PPO 6/7
Pace Analytical Services, Inc.
2225 Riverside Drive
Asheville, NC 28804
Phone: [phone]
Fax: [phone]
Method:
ND
Parameters________
Wet Chemistry
Cyanide. Total. Water
Cyanide
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services Inc
npbr
Project Sample Number: 9296272-002
Matrix: Water
EPA 1664A
mg/1
EPA 335.3
mg/1
EPA 624
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
jAshville Certification IDs
■NC Wastewater
"NC Drinking Water
SC Environment
■FL NELAP
Charlotte Certification IDs
NC Wastewater 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627
race Analytical®
www.pacelabs.com
Lab Sample No: 925731820
Client Sample ID: EFFLUENT GRAB
I By
I
Date: 06/23/05
Page: 5 of 6
Report Limit
10.
40
37712
99030
E87648
Results
ND
99
95
103
CAS No.
75-01-4
2037-26-5
460-00-4
1868-53-7
Date Collected: 06/07/05 14:00
Date Received: 06/09/05 16:00
Pace Analytical Services, Inc.
9800 Kincey Avenue, Suite 100
Huntersville, NC 28078
Phone: [phone]
Fax: [phone]Lab Project Number: 9296272
Client Project ID: PPO 6/7
Pace Analytical Services, Inc.
2225 Riverside Drive
Asheville, NC 28804
Phone: [phone]
Fax: [phone]
Units
ug/1
I
Parameters_____________
Vinyl chloride
Toluene-d8 (S)
4-Bromofluorobenzene (S)
Di bromofluoromethane (S)
Project Sample Number: 9296272-002
Matrix: Water
lAshville Certification IDs
Inc Wastewater
'NC Drinking Water
SC Environment
IFL NELAP
Analyzed
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
06/16/05 03:33 DLK
/^'^ce Analytical®
| / www.pacelabs.com
Charlotte Certification IDs
NC Wastewater 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
ndnr
Lab Sample No: 925731820
Client Sample ID: EFFLUENT GRAB
PARAMETER FOOTNOTES
Method 9071B modified to use ASE.
Depending on the moisture content the PRLs can be elevated for all soil samples reported on a dry weight basis.
2-Chloroethyl vinyl ether has been shown to degrade in the presence of acid.
adjusted reporting limit
I
Date: 06/23/05
Page: 6 of 6
ND
NC
J
MDL
(S)
40
37712
99030
E87648
Pace Analytical Services, Inc.
2225 Riverside Drive
Asheville, NC 28804
Phone: [phone]
Fax: [phone]
All pH, Free Chlorine. Total Chlorine and Ferrous Iron analyses conducted outside of ERA recommended immediate
hold time.
Pace Analytical Services, Inc.
9800 Kincey Avenue, Suite 100
Huntersville, NC 28078
Phone: [phone]
Fax: [phone]
Lab Project Number: 9296272
Client Project ID: PPO 6/7
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc
/nphrt
Not detected at or above adjusted reporting limit
Not Calculable
Estimated concentration above the adjusted method detection limit and below the
Adjusted Method Detection Limit
Surrogate
Ashville Certification IDs
NC Wastewater
NC Drinking Water
SC Environment
FL NELAP
race Analytical®
www.pacelabs.com
Charlotte Certification IDs
NC Wastewater 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627
I
Subcontracted Results
i
i
*
i
Pace Project #
“Thcul 11
This report includes
page 1 to ia
Analytical Results
I
9296272For:
ample ID Parameter Gust ID Result Units Method Date Analyzed Analyst
050610-9 Thallium-Fur/liquid eff comp <0.001 mg/L SM3113B 6/21/05 MD
Respectfully submitted,
\' -vV ,
I
I
6/10/2005
6/21/2005
STATESVILLE
ANALYTICALEntered
Reported:
Sample Remark
I
Dena Myers
■NO Cert #440,
■NCDW Cert #37755,
ERA NC00909
P.O. Box 228 • Statesville, North Carolina 28687 • 704/872/4697
I
Pace Analytical
9800 Kincey Avenue, Sui
Huntersville NC 28078
of
-
Xddress Copy To:Company Name:
Address:
□ GA □ IL:mail To:□ IN □ Ml □ NCPurchase Order No.:Pace Quote Reference:
□ OH □ SC □ Wl
’hone Fax Project Name:Pace Project Manager:
////////tequested Due Date/TAT:Project Number:Pace Profile #:
Valid Matrix CodesSection D
Preservatives
SAMPLE ID
COLLECTED
COMPOSITE START COMPOSITE END/GRAB
DATE TIME DATE TIME
■
iditional Comments:
‘A7/
, i .'
PRINT Name of SAMPLER:
REVERSE SIDE FOR INSTRUCTIONS SIGNATIIRF nf <tlMPi co.
REGULATORY AGENCY
SITE LOCATION
ACCEPTED BY/ AFFILIATION IME
SAMPLER NAME AND SIGNATURE
£ ,
SAMPLE CONDITION
'' 5
CO (N 5
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□ NPDES
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WT vwv
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SL
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AR
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One Character per box.
(A-Z. 0-9 /.-)
Samples IDs MUST BE UNIQUE
Section C
Invoice Information:
Attention:
k
5
□ DRINKING WATER
□ Other
Pace Project Number
Lab I.D
CM
03 z
Ia.
D ±'
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8 Q
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Section B
Required Project Information:
Report To:
Filtered (Y/N)
Requested
Analysis: /
Page:
895423
I
Section A
Required Client Information:
Company
So
>. <3
§ ?w ra I
Required Client Information MATRIX CODE
DRINKING WATER DW
WATER
WASTE WATER
PRODUCT
SOIL/SOLID
OIL
WIPE
AIR
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TISSUE
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The Chain-of-Custody is a LEGAL DOCUMENT. All relevant fields must be completed accurately.
2 8 |
RELINQUISHED BY / AFFILIATION DATE I TIME
/^^ace Analytical'
6° °
s
/^ace Analytical'
of
ddress Copy To:Company Name:
Address:
□ NCmail To:Purchase Order No.:Pace Quote Reference:
hone Fax Project Name:Pace Project Manager:
7///////equested Due Date/TAT:Project Number:
Section D Preservatives
SAMPLE ID
V ■-
4COLLECTED
COMPOSITE START COMPOSITE END/GRAB
DATE TIME DATE TIME
!£/'C./ - /o.
I1
■ditional Comments:T
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?
5
PRINT Name of SAMPLER:
8
REGULATORY AGENCY
SITE LOCATION
RELINQUISHED BY I AFFILIATION DATE TIME
SAMPLER NAME AND SIGNATURE
z
z>
o
z
□ NPDES
□ UST
o
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□GA
□OH
□ GROUND WATER
□ RCRA
□ IL
□ SC
□ IN
□ Wl
WT
WW
P
SL
OL
WP
AR
OT
TS
Pace Project Number
Labl.D
Section A
equired Client Information:
ompany
One Character per box.
(A-Z, 0-9 /.-)
Samples IDs MUST BE UNIQUE
Section C
Invoice Information:
Attention:
i;
Q.
D
□ DRINKING WATER
□ Other
Section B
Required Project Information:
Report To:
5 < I co
z I <
Q
O
O
x
l
z>
Filtered (Y/N)
Requested
Analysis:
Page:
895423
MPLE CONDITION
z>I
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Pace Profile #:
g o
CM
X
Valid Matrix Codes
Required Client Information MATRIX CODE
DRINKING WATER DW
WATER
WASTE WATER
PRODUCT
SOIUSOLID
OIL
WIPE
AIR
OTHER
TISSUE
0.
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ACCEPTED BY/AFFILIATION DATE| TIME E?
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ij-iuM-ssr i vfm i i Miiaiyucai rxequest Document
The Chain-of-Custody is a LEGAL DOCUMENT. All relevant fields must be completed accurately.
□ Ml QMN
□ OTHER
5
§
>. o
I
I
Enviroment 1
I
0.5
0.1
ug/1
I
0.005
I
Page 1
Analytical
Method
SM4500NH3F
Quantitation
Level
Number
of
samples
1
Town Of Clayton
Facility Name Little Creek Water Reclamation Facility
Date of sampling_10/ 12/06.
Analytical Laboratory
Parameter________________
Ammonia (as N)________ ___
Dissolyedoxygen___________
Nitrate/Nitrite_____________
Total Kj eld ahi nitrogen______
Total Phosphorus__________
Total dissolved solids_______
Hardness_________________
Chlorine (total residual, TRC)
Oil and grease_____________
Metals (total recoverable), cyanide and total phenols
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Grab
Grab
Antimony
Arsenic
Beryllium
Cadmium
Chromium
Mercury
Nickel
Selenium
Silver
Thallium
Zinc_______________________
Cyanide___________________
Total phenolic compounds
Volatile organic compounds
Acrolein___________________
Acrylonitrile _________
Benzene___________________
B romoform_______________
Carbon tetrachloride________
Chlorobenzene
Chlorodibromomethane
Chloroethane______________
2-chloroethylvinyl ether
Chloroform________________
Dichlorobromomethane
1,1 -dichloroethane
1,2-dichloroethane
Trans-1,2-dichloroethylene
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
EPA 204.2
EPA 201.2
EPA 200.7
EPA 213.2
EPA 200.7
EPA 200.7
EPA 239.2
EPA 351.2
EPA 351.2
EPA 351.2
EPA 351.2
EPA 351.2
EPA 351.2
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
5
0.2
10
10
5
10
Units of
Measure
ment
mg/1
mg/1
mg/1
mg/1
mg/1
mg/1
mg/1
ug/1
mg/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
Ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
T
2
1
_1
1
1
1
2_
2_
1
1
1
_1
1
1
1
1
1
1
1
1
1
_!
1
_1
1
1
1
1
1
1
1
Copper
Lead
EPA 353.2
EPA 351.2
SM4500P
SM 2540 D
SM2340C
SM 4500 Cl-D
SM5520B
SM4500CN-E
SM510A&E
Sample
Result
<0.5
7.9
0.67
4.14
3.99
________1_
______56
______12
3.9
ORC James Warren
Phone [phone]
Sample Type
Composite
____Grab_____
Composite
Composite
Composite
Composite
Composite
11/27/06 Grab
Grab
<100
<50
<5
<5
__<5
__<5
<5|ug/l
<10
<5
__<5
<5
<5
<5
<5
___<3
___<5
___<1
__<1
__<5
___14
___<5 ug/1
<0.2
<10
<10
___<5
<10
___94
<0.005|mg/l
2 ug/1
ug/1
100
50
5
5
________5
5
________5
_______10
5
5
5
________5
________5
5
3
5
1
1
5
I
I
I
I
Page 2
<10
<10
'•aaxo::'- ', xn
v-?-
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
EPA 625
~ EPA 625
‘ EPA 625
‘ EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
<20
<10
<10
<10
<50
<10
<10
<10
<50
<10
<10
j_
j_
j_
j
j
_1
_1
2_
i_
_i_
_i_
T|
_i
_i
_i_
_i_
i
i
_i
2
j_
j_
j_
_i
2_
_i_
2_
2_
i
ug/l
ug/1
<10 ug/l
<100 ug/1
ug/l
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
10 ug/1
ug/1
ug/1
ug/1
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<20
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
1.1 -dichloroethylene
1.2- dichloropropane
1.3- dichloropropylene
Ethylbenzene
Methyl bromide
Methyl chloride
Methylene chloride
1.1.2.2- tetrachloroethane
Tetrachloroethylene
Toluene
1.1.1 -trichloroethane
1.1.2- trichloroethane
Trichloroethylene
Vinyl chloride
5
5
5
5
10
10
10
_5
_5
_5
5
_5
_5
10
_ 201
___________10__
___________10__
___________10__
___________50__
___________10__
___________10
___________10__
___________50 ~
___________10__
~| 10
- ug/1
10
10__
10__
100__
10
10__
10__
10
10
10__
10
10
20
10___
10___
10
10
<5
<5
<5
<5
<10
<10
<10
<5
<5
<5
<5
<5
<5
<10
P-chloro-m -creso
2-chlorophenol ~
2.4- dichlorophenol
2.4- dimethylphenol
4.6- dinitro-o-cresol
2.4- dinitrophenol
2-nitrophenol
4-nitrophenol ~
Pentachlorophenol
Phenol
2.4.6- trichlorophenoI
Base-neutralcompounds
Acenaphthene ~
Acenaphthylene
Anthracene ' ’
Benzidine
Benzo(a)anthracene ” ~
Benzo(a)pyrene
3,4 benzofluoranthene
Benzo(ghi)perylene
Benzo(k)fluoranthene
Bis (2-chloroethoxy) methane
Bis (2-chloroethyl) ether
Bis (2-chloroisopropyl) ether
Bis (2-ethylhexyl) phthalate
4-bromophenyl phenyl ether
Butyl benzyl phthalate
2 -chloronaphthalene
4-chlorophenyl phenyl ether
u,
I
Ai
I Date
I
Page3
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
IePA 625
~ EPA 625
EPA 625
" EPA 625
~ EPA 625
~ EPA 625
EPA 625
" EPA 625
EPA 625
EPA 625
' EPA 625
~ EPA 625
‘ EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
10
10
10
10
10
10,
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10 ‘
10 ‘
_10 ‘
12
12
10 ’
12"
10 ’
Ug/I
ug/1
ug/1
ug/1
ug/1
ug/1
ug/l
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
Ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
Chrysene
Di-n-butyl phthalate
Di-n-octyl phthalate
Dibenzo(a,h)anthracene
1.2- dichlorobenzen e
1.3- dichlorobenzene
1.4- dichlorobenzene
3.3- dichlorobenzid ine
Diethyl phthalate
Dimethyl phthalate
2.4- dmitrotoluene
2,6-dinitro toluene
1,2-diphenylhydrazine
Fluoranthene
Fluorene
H exachlorobenzene
Hexachlorobutad iene
H exachlorocyclo- p entadiene
Hexachloroethane
Indeno(l,2,3-cd)pyrene
Isophorone
Naphthalene
Nitrobenzene
N-nitrosodi-n-propylamine
N-nitrosodimethylamine
N-nitrosodiphenylamine
Phenanthrene
Pyrene
1.2,4.-trichlorobenzene
^nature
of
■ ___________________________________________
_______1_
’_______1_
■__________1_
_______1_
_______I_
___£
_______1_
______1_
______1_
______!_
______1_
______1_
______1_
______1_
______1_
___£
______1_
______1
______1_
______1_
______1_
______1_
______1_
______1_
______1_
______1_
______1_
______1
1
<10
<10
<10
<10|ug/l
<10
<10
<10
<10
<10|ug/l
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
L or-'’ c _ _
Icertify under penalty of law that this document and all attachments were prepared under my directior
gX rX With a “ t0 deSlgn “ aSSUre that ^^dPperdonnel prZeHy
mZaX svstem o ’th BaS<id °n °< Pe-onsZat
infTr r t PerSOnS ectly resP°ns‘bcl for gathering the information the
awaXZereT p0 kn°Wledge aCCUrate “d “
nos K i n, rr SIgniflcaIlt penalties for submitting false information, including the
possibility of fines and imprisonment for knowing violations.
tfcwszed Representative name
I
I
I
ID :
ID#:890 C
I
REVIEWED BY:
I Effluent
PARAMETERS
Analysis
Date Analyst
Method
Code
10/19/06
10/18/06
10/18/06
10/17/06
10/19/06
10/13/06
10/24/06
10/24/06
10/24/06
10/26/06
10/24/06
10/24/06
10/25/06
10/19/06
10/24/06
10/27/06
10/24/06
10/23/06
10/23/06
EPA200.7
EPA200.7
EPA245.1
EPA200.7
EPA200.7
EPA279.2
EPA289.1
EPA351.2
EPA353.2
SM5520B
SM510A&B
SM4500 CN-E
SM2340C
DATE COLLECTED: 10/12/06
DATE REPORTED : 10/30/06
Jotal Kjeldahl Nitrogen, mg/1
Nitrate-Nitrite, mg/1
■iil & Grease, mg/1
Bienol, ug/1
Total Cyanide, mg/1
Total Hardness, mg/1
■ntimony, ug/1
Wrsenic, ug/1
Beryllium, ug/1
Hadmium, ug/1
popper, ug/1
Total Chromium, ug/1
^ead, ug/1
■ercury, ug/1
nickel, ug/1
ielenium, ug/1
■her, ug/1
■nallium, ug/1
iinc, ug/1
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON ,NC 27528
JKD
JKD
SMA
JKD
SEJ
TRB
CMF EPA204.2
CMF EPA206.2
LFJ EPA200.7
CMF EPA213.2
LFJ
LFJ
CMF EPA239.2
ADD
LFJ
CMF EPA270.2
LFJ
CMF
ADD
4.14
0.67
3.9
2
<0.005
56
<3.0
<5.0
<1.0
<1.0
14
<5.0
<5.0
<0.2
< 10
< 10
<5.0
<1.0
94
Drinking Water ID: 37715
Wastewater ID: 10
I
CLIENT:CLIENT ID:890 C
27528
REVIEWED BY:
I
Effluent
PARAMETERS, ug/l
I
I
I
I
I
I
21.
22.
VOLATILE ORGANICS
EPA METHOD 624
ANALYST:
DATE COLLECTED:
DATE ANALYZED:
DATE REPORTED:
<10.00
<10.00
<10.00
<10.00
<5.00
<5.00
<10.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
MAO
10/12/06
10/20/06
10/30/06
29.
30.
31.
Drinking Water ID: 37715
Wastewater ID: 10
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
1. Chloromethane
2. Vinyl Chloride
3. Bromomethane
4. Chloroethane
5. Trichlorofluoromethane
6. 1,1-Dichloroethane
7. Methylene Chloride
8. trans-l,2-Dichloroethene
9. 1,1-Dichloroethene
10. Chloroform
11. 1,1,1-Trichloroethane
12. Carbon Tetrachloride
13. Benzene
14. 1,2-Dichloroethane
15. Trichloroethene
16. 1,2-Dichloropropane
17. Bromodichloromethane
18. 2-ChloroethyIvinyl Ether
19. Cis-1,3-Dichloropropene
20. Toluene
trans-1,3-Dichloropropene
1.1.2- T richloroethane
23. Tetrachloroethene
24. Dibromochloromethane
25. Chlorobenzene
26. Ethylbenzene
27. Bromoform
28. 1,1,2,2-Tetrachloroethane
1.3- Dichlorobenzene
1.4- Dichlorobenzene
1.2- Dichlorobenzene
EunwoErofTODiTDoroG 1 OoiieoffipwaM
CLIENT:CLIENT ID;890 X
I 27528
REVIEWED BY:
I Effluent
PARAMETERS, ug/1
I
I
I
Laboratof^An^^^^^li^^nt^onsultants
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
VOLATILE ORGANICS
EPA METHOD 624
10/20/06
11/15/06
<10.00
<10.00
<10.00
<10.00
<5.00
<5.00
<10.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<100.00
<50.00
PHONE [phone]
FAX [phone]I GREENVILLE, N.C. 27835-7085
1. Chloromethane
2. Vinyl Chloride
3. Bromomethane
4. Chloroethane
5. Trichlorofluoromethane
6. 1,1-Dichloroethane
7. Methylene Chloride
8. trans-l^-Dichloroethene
9. 1,1-Dichloroethene
10. Chloroform
11. 1,1,1-Trichloroethane
12. Carbon Tetrachloride
13. Benzene
14. 1,2-Dichloroethane
15. Trichloroethene
16. 1,2-Dichloropropane
17. Bromodichloromethane
18. 2-ChIoroethylvinyl Ether
19. cis-l,3-Dichloropropene
20. Toluene
21. trans-l,3-Dichloropropene
22. 1,1^-Trlchloroethane
23. Tetrachloroethene
24. Dibromochloromcthane
25. Chlorobenzene
26. Ethylbenzene
27. Bromoform
28. 1,1,2,2-Tetrachloroethane
29. 13-Dichlorobenzene
30. 1,4-Dichlorobenzene
31. 1,2-Dichlorobenzene
32. Acrolein
33. Acrylonitrile
ANALYST: MAO
DATE COLLECTED; 10/12/06
DATE ANALYZED:
DATE REPORTED:
CLIENT:CLIENT ID:890 C
27528 Page: 1
REVIEWED BY:
I
Effluent
PARAMETERS, ug/1
I
I
I
I
SEMIVOLATILE ORGANICS
EPA METHOD 625
ANALYST:
DATE COLLECTED:
DATE EXTRACTED:
DATE ANALYZED:
DATE REPORTED:
CHS
10/12/06
10/18/06
10/20/06
10/30/06
Drinking Water ID: 37715
Wastewater ID: 10
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<20.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<50.00
<50.00
<10.00
<10.00
<10.00
<10.00
<50.00
<10.00
<10.00
<10.00
<50.00
<10.00
<10.00
<10.00
<10.00
<100.00
<10.00
<10.00
<10.00
<10.00
<10.00
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
34. 4,6-Dinitro-2-Methylphenol
35. N-Nitrosodiphenylamine
36. 4-Bromophenyl Phenyl Ether
37. Hexachlorobenzene
38. Pentachlorophenol
39. Phenanthrene
40. Anthracene
41. Di-N-Butylphthalate
42. Fluoranthene
43. Benzidine
44. Pyrene
45. Butylbenzylphthlate
46. Benzo[a]anthracene
47. 3,3-Dichlorobenzadine
48. Chrysene
1. N-Nitrosodimethylamine
2. Phenol
3. Bis(2-Chloroethyl) Ether
4. 2-Chlorophenol
5. 1,3-Dichlorobenzene
6. 1,4-Dichlorobenzene
7. 1,2-Dichlorobenzene
8. Bis(2-Chloroisopropyl) Ether
9. Hexachloroethane
10. N-Nitrosodi-N-Propylamine
11. Nitrobenzene
12. Isophorone
13. 2-Nitrophenol
14. 2,4-Dimethylphenol
15. Bis(2-Chloroethoxy) Methane
16. 2,4-DichlorophenoI
17. 1,2,4-Trichlorobenzene
18. Naphthalene
19. Hexachlorobutadiene
20. 4-Chloro-3-MethyIphenol
21. Hexachlorocyclopentadiene
22. 2,4,6-Trichlorophenol
23. 2-Chloronaphthalene
24. Acenaphthylene
25. Dimethylphthalate
26. 2,6-Dinitrotoulene
27. Acenaphthene
28. 2,4-Dinitrophenol
29. 4-Nitrophenol
30. 2,4-Dinitrotoluene
31. Fluorene
32. Diethylphthalate
33. 4-ChlorophenyI Phenyl Ether
CLIENT:
CLIENT ID:890 C
27528
Page: 2
REVIEWED BY:
EffluentPARAMETERS, ug/1
Bis (2-EthyIhexyl)phthaIate
I
I
I
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
SEMIVOLATILE ORGANICS
EPA METHOD 625
ANALYST:
DATE COLLECTED:
DATE EXTRACTED:
DATE ANALYZED:
DATE REPORTED:
<20.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
CHS
10/12/06
10/18/06
10/20/06
10/30/06
Drinking Water ID: 37715
Wastewater ID: 10
49. F'---- - “
50. Di-N-Octylphthalate
51. Benzo[b]fluoranthene
52. Benzo[k]fluoranthene
53. Benzo [a] pyrene
Indeno(l,2,3-C,d)pyrene54. ] .
55. Dibenzo[a,h]anthracene
56. Benzo[g,h,i]perylene
57. 1,2-Diphenylhydrazine
CH Airs or CUdlUDY KJLCOKD
Page i of 4-
Phone [phone] • Fax [phone] .
CHLORINE NEUTRALIZED AT COLLECTION
CLIENT:890 C Week: 1 pH CHECK (LAB)UV
CONTAINER TYPE, P/GPPGGP P P G G G G G G
CHEMICAL PRESERVATIONCCccDCBAAAEE E
A-NONE D-NAOH
[phone] E-HCL
C-H2SO4 F-ZINC ACETATECOLLECTION
G-NA THIOSULFATESAMPLE LOCATION DATE TIME
■II ii 180ft>0Effluent 13 CLASSIFICATION:
WASTEWATER (NPDES)
□DRINKING WATER
DWQ/GW
□SOUD WASTE SECTION
SAMPLES RECEIVED IN LAB AT °C
COMMENTS:
RECEIVED BY (816.^
ELINQUISHED BY (SIG.)RECEIVED BY (SIG.)
u^j^e a
I >1 ViUlivill A- lilt.
P.O. Box 7085, 114 Oakmont Dr.
Greenville, NC 27858
SAMPLES COLLECTED BY:
(Please Print)
'Oo s
£
0J
a
Q.
5
Q
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3
CL. w
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1 a.
3
Q
3
'O
§
3
fS
1
0* s
<u
§
0
a
O
co cc
i
as
a
3
Q
'O
1a
3
Q
’T
“G”
ELINQUISHED BY (SIG.) (SAMPLI
ELINQUISHED BY (SIG.) /
DATE/TIME
DATE/TIME
-J__
DATE/TIME
I
DATETIME
DATE/TIME
__I
DATE/TIME
I
CHAIN OF CUSTODY MAINTAINED
DURING SHIPMENT/DELIVERY
Y N
LU
2
f
8
o
CC Q
so 10
LU
ig -II
— ^8
n n°neTOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON NC 27528
DISINFECTION
Pj CHLORINE
b-hno3
, RECEIVED BY (SIG.)
TKN
Nitrate-Nitrite n
o VIe>
§o
n 15
W
H>0
>0
>0
W 0
M 0
W 0
I
I X
I
0
■XI
L-'J
Cy
€x
0!
N
'S
3
U1cn
<iM
tn wO\
V
3 c
§
§
00 se o
0
kj
<3»
'S
O
V
r~
V
$
PS§3
h n
h 2 Z
r°
It OU & Grease
■ Phenol
■ Cyanide
O? Hardness
Metals
JI EPA 625
625 Duplicate 1
f|| 625 Duplicate 2
EPA 624 (APA)
||f 624 Duplicate 1
| 624 Duplicate 2
r c >> 3 w p Is
w
u?
00
Enviroment 1
0.1
0.005
<2
<5
Page 1
_3
5
1
2
5
Analytical
Method
SM4500NH3F
Number
of
samples
1
2
i
2
i
ij
j]
il
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Composite
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
5
0.2
10
10
5
1
<100|ug/l
<50
___<5
<5
<5
<5
<5
<10
<5
<5
<5
<5
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
<5|ug/l
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/I
ug/1
ug/1
mg/l
mg/l
ug/1
mg/l
Units of
Measure
ment
mg/l
mg/l
mg/l
mg/l
1
1
1
1
1
1
2j
2j
2]
T]
Ij
H
7]
il
i
i
i
i
i
i
2j
2j
jl
Tj
T]
2j
T]
n
Tl
ug/1
ug/1
<1.0 ug/1
144 ug/1
<0.005 mgTT
ug/1
___<3
<5
<1
__<1,
<5
16
<5
<0.2
<10 ug/1
<10
<5
ORC James Warren
.Phone [phone]
Sample
Result
<0.5
7,6
5.81
1.65
1-76 mg/l
565
______61
______12
< 5
Sample Typ
Composite
Grab
Composite
Composite
Composite
Composite
Composite
8/23/07 Grab
______Grab (
petals (total recoverable), cyanide and total phenols
~|SM 3113 B
_ SM 3113 B
_ EPA 200,7
SM 3113 B
EPA 200.7
_ EPA 200,7
_ SM 3113 B
~ EPA 245.1
EPA 200,7
_ SM 3113 B
EPA 200,7
~SM 3113 B
’ SM 3113 B
_ SM4500CN-E
[SMSIOA&E
EPA 624
EPA 624
EPA 624
EPA 624 '
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624 "
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624 ~
EPA 624
(Antimony
[Arsenic
(Beryllium
[Cadmium
[Chromium
[Copper
[Lead
[Mercury
(Nickel
[Selenium
(Silver ~
[Thallium
[Zinc “
[Cyanide
Total phenolic compounds
[Volatile organic compounds
[Acrolein
[Acrylonitrile
[ Benzene
[Bromoform
Carbon tetrachloride
[Chlorobenzene
| Chlorodibromom ethane
[ Chloroethane
[2-chloroethylvinyl ether
[Chloroform ~
[ Dichlorobromom ethane
11,1-dichloroethane
1,2-dichloroethane
Trans-1,2-dichloroethylene
ug/1
100
_______50
_________5
________5
5
5
5
10
________5
5
________5
________5
5
5
[Parameter
[Ammonia (as N)
(Dissolved oxygen
iNitrate/Nitrite “
Total Kjeldahl nitrogen
[Total Phosphorus
[Total dissolved solids
[Hardness ~
Chlorine (total residual, TRC)
Oil and grease
Town Of Clayton
Facility Name Lime Creek Water Reclamation Facility
Date of sampling 9/06/07.
Analytical Laboratory
EPA 353,2
EPA 351,2
SM4500P
SM 2540 D
SM2340C
SM4500 Cl-D |
EPA1664A |
3 ug/1
Quantitation
Level
______ 0.5
I
Sample Type
GrabIGrab
Grab
ug/1
Grab
Grab
Grab
ug/1
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Page 2
Grab
Grab
Sample
Result
Number
of
samples
Units of
Measure
ment
Benzidine
Benzo(a)anthracene
Benzo(a)pyrene
3,4 benzofluoranthene
Benzo(ghi)perylene
Benzo(k)fluoranthene
Bis (2-chloroethoxy) methane
Bis (2-chloroethyl) ether
Bis (2-chloroisopropyl) ether
Bis (2-ethylhexyl) phthalate
4-bromophenyl phenyl ether
Butyl benzyl phthalate
2-chloronaphthalene
4-chlorophenyl phenyl ether
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Analytical
Method
KPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 624
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
10
10
10
100
10
10
10
10
10
10
10
10
20
10
10
10
10
20
10
10
10
50
50
10
50
50
10
10
5
5
5
5
10
10
10
5
5
5
5
5
5
10
ug/1
ug/1
ug/1
<5 ug/1
<5 ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
ug/1
2_
_1
2_
2_
2_
J_
j_
1
1_
2_
1
1
T
j_
2
i
£
1
2
2
2
2
2
2
2
2
2
2
2
2
2
i
1.1.1- trichloroethane
1.1.2- trichloroethane
Trichloroethylene
Vinyl chloride
Acid-extractable compounds
P-chloro-m-creso
2-chlorophenol
2.4- dichlorophenol
2.4- dimethylphenol
4.6- dinitro-o-cresol
2.4- dinitrophenol
2-nitrophenol
4-nitrophenol
Pentachlorophenol
Phenol
2.4.6- trichlorophenol
Base-neutral compounds
Acenaphthene
Acenaphthylene
Anthracene
Parameter
Volatile organic compounds (Cont.)
1.1- dichloroethylene
1.2- dichloropropane
1.3- dichloropropylene
Ethylbenzene
Methyl bromide
Methyl chloride
Methylene chloride
1,1,2,2-tetrachloroethane
Tetrachloroethylene
Toluene
<20
<10
<10
<10
<50
<50
<10|ug/l
<50
<50
<10
<10
Quantitation
Level
ug/1
<10
<10
<10
<100
<10
<10
<10
<10
<10
<10
<10
<10
<20
<10
<10
<10
<10
<5
<5
<10
<5
<5
<5
<5lug/l
<10 ug/1
<10 ug/1
<10
<5
<5
I
Parameter
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
I
Page 3
I
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Number
of
samples
Sample
Result
Units of
Measure
ment
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
Grab
10
10
10
10
10
10
10
10
10
10
10
10
10
10
22
12
12
22
12
12
22
12
22
22
12
22
12
22
10
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
ug/l
_1
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
i
2
2
2
i
Analytical
Method
EPA 625
|EPA 625
~ EPA 625
~ EPA 625
~ EPA 625
’ EPA 625
EPA 625
" EPA 625
~ EPA 625
~ EPA 625
EPA 625
~ EPA 625
~ EPA 625
~ EPA 625
' EPA 625
' EPA 625
EPA 625
' EPA 625
EPA 625
' EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
EPA 625
IEPA 625
<10
<10
<10
<10
<10|ug/l
<10 ug/l
<10
<10
<10
<10
<10|ug/l
<10
<10
<10|ug/l
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
<10
Quantitation
Level
ug/l
I certify under penalty of law that this document and all attachments were prepared under my directior
and supervision in accordance with a system to design to assure that qualified perdonnel properly
gather and evaluat the information submitted. Based on my inquiry of the person or persons that
manage the system, or those persons directly responsibel for gathering the information, the
information submitted is , to the best of my knowledge and belief, true, accurate and complete. I am
aware that there are significant penalties for submitting false information, including the
possibility of fines and imprisonment for knowing violations.
(Z^L Uli 2Date f ■
Autoriked Representative name
Sample Type
Base-neutral compounds (cont.)
Chrysene
Di-n-butyl phthalate
Di-n-octyl phthalate
Dibenzo(a,h) anthracene
1.2- dichlorobenzene
1.3- dichlorobenzene
1.4- dichlorobenzene
3.3- dichlorobenzidine
Diethyl phthalate
Dimethyl phthalate
2.4- dinitrotoluene
2,6-dinitrotoluene
1,2-diphenylhydrazine
Fluoranthene
Fluorene
H exach lorobenzene
Hexachlorobutadiene
Hexachlorocyclo-pentad iene
Hexachloroethane
lndeno(l,2,3-cd)pyrene
Isophorone
Naphthalene
Nitrobenzene
N-nitrosodi-n-propylam ine
N-nitrosodimethylamine
N -nitrosodiphenylam ine
Phenanthrene
Pyrene
1,2,4,- trichlorobenzene
ID#:890 C
REVIEWED BY:
Effluent
PARAMETERS Analyst
I
Leiboratory Analyses— Environmentei: f> su/-....
Total Kjeldahl Nitrogen, mg/1
Nitrate-Nitrite, mg/1
Oil & Grease (HEM), mg/1
Phenol, ug/1
Total Cyanide, mg/1
Total Hardness, mg/1
Total Dissolved Residue, mg/1
Antimony, ug/1
Arsenic, ug/1
Beryllium, ug/I
Cadmium, ug/1
Copper, ug/1
Total Chromium, ug/1
Lead, ug/1
Mercury, ug/1
Nickel, ug/1
Selenium, ug/1
Silver, ug/1
Thallium, ug/1
Zinc, ug/1
Analysis
Date
Method
Code
09/10/07
09/12/07
09/13/07
09/10/07
09/14/07
09/07/07
09/13/07
09/18/07
09/12/07
09/11/07
09/11/07
09/11/07
09/11/07
09/13/07
09/13/07
09/13/07
09/14/07
0p/ll/07
09/10/07
09/14/07
SSR
SSR
SEJ
SEJ
SEJ
TRB
TRB
CMF
CMF
LFJ
CMF
LFJ
LFJ
CMF
ADD
LFJ
CMF
LFJ
CMF
ADD
EPA351.2
EPA353.2
EPA1664A
SM510A&B
SM4500 CN-E
SM2340C
SM2540C
SM3113B
SM3113B
EPA200.7
SM3113B
EPA200.7
EPA200.7
SM3113B
EPA245.1
EPA200.7
SM3113B
EPA200.7
SM3113B
SM3111B
DATE COLLECTED:
DATE REPORTED :
09/06/07
09/18/07
3T,
10
Drinking Water ID:
Wastewater ID:
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON ,NC 27528
1.65
5.81
<5.0
<2
<0.005
61
565
<3.0
<5.0
<1.0
<1.0
16
<5.0
<5.0
<0.2
< 10
< 10
<5.0
<1.0
144
890 CCLIENT ID:CLIENT:
27528
REVIEWED BY:
7
Effluent
PARAMETERS, ug/1
I
Lsboratoi'y Analyses — Envir^ni'''>eni&: consultant.I
1. Chloromethane
2. Vinyl Chloride
3. Bromomethane
4. Chloroethane
5. Trichlorofluoromethane
6. 1,1-Dichloroethane
7. Methylene Chloride
8. trans-l,2-Dichloroethene
9. 1,1-Dichloroethene
10. Chloroform
11. 1,1,1-Trichloroethane
12. Carbon Tetrachloride
13. Benzene
14. 1,2-Dichloroethane
15. Trichloroethene
16. 1,2-Dichloropropane
17. Bromodichloromethane
18. 2-Chloroethylvinyl Ether
19. cis-l,3-Dichloropropene
20. Toluene
21. trans-l,3-Dichloropropene
22. 1,1,2-Trichloroethane
23. Tetrachloroethene
24. Dibromochloromethane
25. Chlorobenzene
26. Ethylbenzene
27. Bromoform
28. 1,1,2,2-Tetrachloroethane
29. 1,3-Dichlorobenzene
30. 1,4-Dichlorobenzene
31. 1,2-Dichlorobenzene
32. Acrolein
33. Acrylonitrile
VOLATILE ORGANICS
EPA METHOD 624
09/11/07
09/18/07
Drinking Water ID: 377
Wastewater ID: 10
<10.00
<10.00
<10.00
<10.00
<5.00
<5.00
<10.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<100.00
<50.00
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
ANALYST: MAO
DATE COLLECTED: 09/06/07
DATE ANALYZED:
DATE REPORTED:
I
CLIENT:CLIENT ID:890 C
27528 Page:
REVIEWED BY:
Effluent
PARAMETERS, ug/l
;wn:'a sultar/t
SEMIVOLATILE ORGANICS
EPA METHOD 625
ANALYST:
DATE COLLECTED:
DATE EXTRACTED:
DATE ANALYZED:
DATE REPORTED:
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<20.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<50.00
<50.00
<10.00
<10.00
<10.00
<10.00
<50.00
<10.00
<10.00
<10.00
<50.00
<10.00
<10.00
<10.00
<10.00
<100.00
<10.00
<10.00
<10.00
<10.00
<10.00
CHS
09/06/07
09/12/07
09/12/07
09/18/07
Drinking Water ID: 37
Wastewater ID: 10
Laboratory Ar'alys&s — Enc. or.
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
1. N-Nitrosodimethylamine
2. Phenol
3. Bis(2-Chloroethyl) Ether
4. 2-Chlorophenol
5. 1,3-Dichlorobenzene
6. 1,4-Dichlorobenzene
7. 1,2-DichIorobenzene
8. Bis(2-Chloroisopropyl) Ether
9. Hexachloroethane
10. N-Nitrosodi-N-Propylamine
11. Nitrobenzene
12. Isophorone
13. 2-Nitrophenol
14. 2,4-DimethylphenoI
15. Bis(2-Chloroethoxy) Methane
16. 2,4-Dichlorophenol
17. 1,2,4-Trichlorobenzene
18. Naphthalene
19. Hexachlorobutadiene
20. 4-Chloro-3-Methylphenol
21. Hexachlorocyclopentadiene
22. 2,4,6-Trichlorophenol
23. 2-Chloronaphthalene
24. Acenaphthylene
25. Dimethylphthalate
26. 2,6-Dinitrotoulene
27. Acenaphthene
28. 2,4-Dinitrophenol
4-Nitrophenoi29. 4-Nitrophenoi
30. 2,4-Dinitrotoluene
31. Fluorene
32. Diethylphthalate
33. 4-Chlorophenyl Phenyl Ether
34. 4,6-Dinitro-2-MethylphenoI
35. N-Nitrosodiphenylamine
36. 4-Bromophenyl Phenyl Ether
37. Hexachlorobenzene
38. Pentachlorophenol
39. Phenanthrene
40. Anthracene
41. Di-N-Butylphthalate
42. Fluoranthene
43. Benzidine
44. Pyrene
45. Butylbenzylphthlate
46. Benzo[a]anthracene
47. 3,3-Dichlorobenzadine
48. Chrysene
1
I n
I
08f
890 CCLIENT ID:CLIENT:
MR.
Page:27528
REVIEWED BY:
Effluent
PARAMETERS, ug/1
Laboratory Analyse? — Envir, n .lental Uo:\ ult---I
SEMIVOLATILE ORGANICS
EPA METHOD 625
ANALYST:
DATE COLLECTED:
DATE EXTRACTED:
DATE ANALYZED:
DATE REPORTED:
CHS
09/06/07
09/12/07
09/12/07
09/18/07
49. Bis(2-Ethylhexyl)phthalate
50. Di-N-Octylphthalate
51. Benzo[b]fluoranthene
52. Benzo[k]fluoranthene
53. Benzo [ajpyrene
54. Indeno(l,2,3-C,d)pyrene
55. Dibenzo[a,h]anthracene
56. Benzo[g,h,i]perylene
57. 1,2-Diphenylhydrazine
<20.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
LIOllWFOEfflllLfDOfJDtt
Drinking Water ID: 37
Wastewater ID: 10
j ij
TOWN OF CLAYTON
JAMES WARREN
P.O. BOX 879
CLAYTON, NC
I
1 1
890 C Week: 1 O' Li 7l/T'
p p G G P P P G G G G G G
c c c c D C A A A A E E E
[phone]
Effluent 9/&/o^ /VP 14
X
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON NC 27528
ir,
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ID#;890 C
I
I
REVIEWKD BY;
I
Effluent
PARAMETERS
Laboratory Analyses — Environmental ConsultantsI
TOWN OF CLAYTON
MR. JAMES WARRKN
P.O. BOX 879
CLAYTON ,NC 27528
Analysis
Date Analyst
09/10/07
09/12/07
09/13/07
09/10/07
09/14/07
09/07/07
09/13/07
09/18/07
09/12/07
09/11/07
09/11/07
09/11/07
09/11/07
09/13/07
09/13/07
09/13/07
09/14/07
09/11/07
09/10/07
09/14/07
SSR
SSR
SEJ
SEJ
SEJ
TRB
TRB
Method
Code
DATE COLLECTED! 09/06/07
DATE REPORTED ; 09/18/07
-Total KjeldaM Nitrogen, mg/1
'Nitrate-Nitrite, mg/l
-On & Grease (HEM), mg/1
4»heno), ug/1
>Total Cyanide, mg/1
-Total Hardness, mg/1
Total Dissolved Residue, mg/1
"Antimony, ug/1
-Arsenic, ug/1
-Beryllium, ug/1
-Cadmium, ug/1
-Copper, ug/1
•Total Chromium, ug/1
^Lcad, ug/1
-Mercury, ug/1
-Nickel, ug/1
-Seleniuni, ug/I
-Silver, ug/1
-Thallium, ug/1
.Zinc, ug/1
JP.O. BOX 7085, 114 OAKMONT DRIVE
■GREENVILLE, N.G. 27835-7085
PHONE [phone]'
FAX [phone]
EPA351.2
EPA353.2
EPA1664A
SM510A&B
SM4500 CN-E
SM234OC
SM2540C
CMF SM3113B
CMF SM3113B
LFJ EPA200.7
CMF SM3113B
LFJ
LFJ
EPA200.7
EPA200.7
CMF SM3113B
ADD EPA245.1
LFJ EPA200.7
CMF SM3113B
LFJ EPA200.7
CMF SM3113B
ADD SM3111B
1.65
5.81
<5.0
<2
<0.005
61
565
<3.0
<5.0
<1.0
<1.0
16
<5.0
<5.0
<0.2
< 10
< 10
<5.0
<1.0
144
1
390 CCLIZNT TDxCLIENT:
I 27528
REVIEWED BY:I
Effluent
PARAMETERS, ug/1
I
I
p; b'-o'*'-0
Laboratory Analyses — Environmental Consultants
I
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
VOLATILE ORGANICS
ERA METHOD 624
ANALYST:
DATE
DATE ANALYZED:
DATE REPORTED:
PHONE [phone]
FAX [phone]
<10.00
<10.00
<10.00
<10.00
<5.00
<5.00
<10.00
<5.00
<5.00
<5.00
<5-00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<5.00
<100.00
<50.00
1], Doi]©@ir[ps)ir§iQ@gD
2P.0. BOX 7085, 114 OAKMONT DRIVE
■SREENVILLE, N.C. 27835-7085
1. Chlorometbane
4. Vinyl Chloride
3. Bromomethane
mJ. Chloroetbane
5. Tricblorofluoroniethane
-€. 1,1-Dichloroethane
-7. Methylene Chloride
-9. trans-l,2-Dichloroethene
9. 1,1-Dlchloroethene
•10. Chloroform
-11. 1,1,1-Trlchloroethane
-12. Carbon Tetrachloride
-13. Benzene
\T4. 1,2-Dichloroethane
15. Trichloroethene
«4'6. 1,2-Dichloropropane
rtf. Bromodichloromethane
'-18. 2-CbIoroethylvinyl Ether
19. cis-l,3-D»ehloropropene
v20. Toluene
21. traiis-l,3-Dichloropropenc
-12. 1,14-Trichloroetbane
23. Tetrachloroethene
44. Dibromochloro methane
.23. Chlorobenzene
-26. Ethylbenzene
—27. Bromoform
^28. 14,2,2-Tetrachloroethane
-29. 1,3-Dichlorobenzene 6*^
-30. 1,4-Dichlorobenzene u x-i
-31. 1,2-Dichlorobenzene
«^2. Acrolein
^33. Acrylonitrile
MAO
COLLECTED: 09/06/07
09/11/07
09/18/07
CLIENT :CLIENT ID;890 C
I 27528 Paga: 1
REVIEWED BY;
Effluent
PARAMETERS, ug/1
I
Laborstory Analyses — Environmental Consultants
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
SEMIVOLATILB ORGANICS
EPA METHOD 625
ANALYST:
DATE COLLECTED:
DATE EXTRACTED:
DATE ANALYZED:
DATE REPORTED:
CHS
09/06/07
09/12/07
09/12/07
09/18/07
PHONE [phone]
FAX [phone]
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<20.00
<10.00
<10.00
<10.00
< 10.00
<10.00
< 10.00
<10.00
<50.00
<50.00
<10.00
<10.00
<10.00
<10.00
<50.00
<10.00
<10.00
<10.00
<50.00
<10.00
<10.00
<10.00
<10.00
<100.00
<10-00
<10.00
<10.00
<10.00
<10.00
x-T. N-NitrosodiniEthylaniine
-4, Phenol
^-3. .Bis(2-Chloroethyl) Ether
-4. 2-Chloro phenol
•5; 1,3-Dichlorobenzene
—6, 1,4-Dichlorobenzene
"Tv- 1,2-DichIorobenzene
-8r Bis(2-Chloroisopropyl) Ether
-9. Hexachloroethane
''10. N-Nitrosodi-N-Propylandne
-41. Nitrobenzene
-12. Isophorone
13. 2-Nitrophenol
HM. 2,4-Dimethylphenol
-15. Bis(2-Chloroethoxy) Methane
A6. 2,4-Dichlorophenol
-17. 1,2,4-Trichlorobenzcne
-48. Naphthalene
-49. Hexachlorobutadiene
20. 4-Chloro-3-Methylphenol
-21. Hexachlorocydopentadiene
-22. 2,4,6-TrichIorophenol
■23. 2-Chloronaphthalene
-24. Acenaphthylene
-25. Dimethylphthalate
-26. 2,6-Dinitrotoulene
-27. Acenaphthene
-28. 2,4-DinitrophenoI
*29. 4-Nitrophenol
■30. 2,4-Dinitrotoluene
*31. Fluorene
■21. Diethylphthalate
%-33. 4-Chlorophenyl Phenyl Ether
34. 4,6-Dinitro-2-Methylphenol
-35. N-Nitrosodiphenylnmine
--36. 4*Bromophenyl Phenyl Ether
37. Hexachlorobenzene
-38. Pentachlorophenol
-39. Phenanthrene
-40. Anthracene
^41. Di-N-Butylphthalate
•42. Fluoranthene
•-43. Benzidine
-44. Pyrene
-45. Butylbenzylphtblate
. -46. Benzo[a]anthracene
“47. 3,3-Dichlorobenzadine
'^8. Chrysene
— P.O. BOX 7085, 114 OAKMONT DRIVE
■ GREENVILLE, N.C. 27835-7085
1 Erowwmwti 1], DuDsotr^oirgtt®^
890 CCLIENT ID:CLIENT:
I Page: 22752B
REVIEWED BY:
Effluent
PARAMETERS, ug/1
I
Laboratory Analyses — Environmental ConsultantsI
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON, NC
SEMIVOLATILE ORGANICS
EPA METHOD 625
<20.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
<10.00
CHS
09/06/07
09/12/07
09/12/07
09/18/07
ANALYST;
DATE COLLECTED;
DATE EXTRACTED:
DATE ANALYZED:
DATE REPORTED:
—49.
-50.
51.
-62.
^3.
P.O. BOX 7085. 114 OAKMONT DRIVE
■ GREENVILLE, N.C. 27835-7085
Bis (2-Ethylhexyl) phthalate
Di-N-Octylpbthalate
Benzo[b]fluoranthene
Benzo [kjfTuoranthene
Benzo [a] pyrene
-54. Lndeno(l,2,3-C,d)pyrene
-55. Dibenzo [a,hjanthracene
-56. Benzo(g,h,i]perylene
-57. 1,2-DiphenyIhydrazine
.................................
PHONE [phone]
FAX [phone]
1 feWMOTirt 1]ff Dim©© [rp[r©tM
CHAIN OF CUSTODY RECORD 1
Page 1 of 1
DISINFECTIONPhone [phone] • Rix [phone] CHLOAIME NEUTRALIZED AT COLLECTION
Week: 1 pH CHECK (LAB)
P-
CCNTAINERTYPE, P/GPPGGPPGGPGGGG
$CHEMICAL PRESERVATIONCcc c D c A A A A E E E
A-NONE D-NAOH
[phone] B-HNOn E-HCL
c-h2so4 F- ZINC ACETATE
COLLECTION
G- NA THIOSULFATE
SAMPLE LOCATION DATE TIME Z
H 1 1 B 1 8 1 §K H SEffluent14 CLASSIFICATION:
WASTEWATER (NPDES)
RELII
RELINQUISHED BY (SIG.)Rl
L
^Ins^ugnsj^g^lelin^j^form ^gler mg^Jice a comgg|S3m^yi "G"
'^1A7
EDBY(SIG^
Environment I, Inc.
P.O. Box 7085.114 Oakmont Dr.
Greenville. NC 27858
TOWN OF CLAYTON
MR. JAMES WARREN
P.O. BOX 879
CLAYTON NC 27528
0
oo
f 4
CHAIN OF CUSTODY MAINTAINED
DUR I N^SH IPMENT/D ELI VER Y
N
i
SE
SAMPLES COLLECTED BY:
(Please Print)
I
o s J3 Pm §
rl
so
£
C/1
8
■S
s
n
3
o<
3
Q
8 o
*o
£M
n
1 □
Q
ms
1
s
2Jex
Q
§
UJZ Z§g
OH< g
1ex<s
n
V
1
u
SAMPLES RECEIVED IN LAB AT^^_ <C
LU z
8 s
RELINQUISHED BY (SIG.) (SAMFLEB)
ft/
HSHED8Y(SIG)
y
LLf S se
£8 rr _j
o1 o s 8
DATE/TIME
DATETIME
DATETIME
Q SOUt) WASTE SECTION
□ DWQCW
Q| DRINKING WATER
Q| CHLORINE
Q uv
□ NONE
□
CLIENT: 89GC
^7 ITATE/TIME z/--------------
--------------
/ DATETIME
I
__1_J_
REGHVEBBYISIGX
wECEMsD BY (SIG.)
CHAIN OF CUSTODY RECORD
Page of
DISINFECTIONPhone [phone] • Fax [phone] CHLORINE NEUTRALIZED AT COLLECTION
CHLORINE
pH CHECK (LAB)UV
CONTAINER TYPE, P/G
CHEMICAL PRESERVATION
A-NONE D-MAOH
B-HNOn E-HCL
C-H?SO(F-ZINC ACETATE
COLLECTION
G - NATHIOSULFATE
SAMPLE LOCATION DATE TIME
I CLASSIFICATION:
WASTEWATER (NPDES)
□DRINKING WATER
□DWQGW
□SOLID WASTE SECTION
COMMENTS:
RELINQUISHED BV (SIG.)
Instructions for completing this form are on lhe reverse side.
1^11^
Environment T,Inc.
?.O. Box 7085, 114 Oakmont Dr.
Greenville, N(^27^?
’O
Sampler must place a *C’ for composite sample or a "G” for
^g|samr|^gie blrg|^iove|m^ DaMVr reol
£I
I£
SAMPLES COLLECTED BY:
(Please Print)
SAMPLES RECEIVED IN LAB AT^ aC
DATE/DME
I_
DATETIME
DATE/T1ME
I
CUENT:
12 in z
I
8
b *r-
FEUNaJISHED BY^IG.)
RECEIVED BY (SIG.)
E RECEIVED BY (SIGJ
9w z
Ct Oil
w oBg
LU
g o
^8
2 fci:
; 'T^cO <<
CHAIN OF CUSTODY MAINTAINED
DURING S^MENT/DELIVERY
^.0 . g&r 8-7^
fJC 2
DATETIME
\\;ocp
DATETIME —
I
a
I lf NON Li
□
inal wt gm
|% Solids
Town of Clayton
Total Solids Dried at 103-105 C
STM 2540/X?
% RPD = 2 ( A - B ) x 100
A+ B
'o RPD
% total Sol.= ( Dried wt - Dish wt) x 100
samlpe volume (25-50 gms)
sample vol
Dried wt gm
Dish wt gm
.O3H
Diffgm
sample vol
Final wt gm
% Solids
% RPD
Blank
Dried wt gm
Dish wt gm
sample vol
Blank
biff gm
Toxicity Testing Data
I
I
I
i
I
I
I
I
1
I
I
Transform:NO TRANSFORMATI01
Calculated Bl statistic =3.9496 (p-value = 0.5567)
Data PASS Bl homogeneity test at 0.01 level. Continue analysis.
I
I
I
Title:
File:
5)
5)
Critical B = 15.0863
= 11.0705
clayton
clayton
Bartlett's Test for Homogeneity of Variance
(alpha = 0.01, df =
(alpha = 0.05, df =
Transform:NO TRANS FORMAT 101
ANOVA Table
SOURCE DF SS MS F
Between 5 0.0409 0.0082 1.9844
Within (Error)18 0.0741 0.0041
Total 23 0.1150
(p-value = 0.1299)I Critical F
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)
I
1
I
I
df = 5,18)
df = 5,18)
Title:
File :
= 4.2479
= 2.7729
(alpha = 0.01,
(alpha = 0.05,
clayton
clayton
Transform:NO TRANSFORMAT10
Dunnett1s Test TABLE 1 OF 2 Ho:ControlcTreatment
GROUP IDENTIFICATION T STAT
Dunnett critical value = 2.4100 (1 Tailed, alpha = 0.05,df = 5,18)
Transform:NO TRANS FORMAT 101
Dunnett's TestI TABLE 2 OF 2 Ho:Control<Treatment
GROUP IDENTIFICATION
Title:
File :
Title:
File :
DIFFERENCE
FROM CONTROI
1
2
3
4
5
6
NUM OF
REPS
TRANSFORMED
MEAN
4
4
4
4
4
4
MIN SIG DIFF
(IN ORIG. UNITS)
0.1094
0.1094
0.1094
0.1094
0.1094
MEAN CALCULATED IN
ORIGINAL UNITS
% OF
CONTROL
22.9
22.9
22.9
22.9
22.9
-0.0350
0.0275
-0.0525
-0.0060
-0.1003
SI
0 .
1
2
3
4
5
6
control
0.5
1
2
4
8
0.4768
0.5118
0.4493
0.5293
0.4828
0.5770
0.4768
0.5118
0.4493
0.5293
0.4828
0.5770
-0.7712
0.6060
-1.1569
-0.1322
-2.2091
control
0.5
1
2
4
8
clayton
clayton
clayton
clayton
“SERVICE”“ENVIRONMENT”
August 9, 2007
Re: Fat Head Minnow Toxicity Results
To Whom It May Concern:
Please call me directly at 919-553-1536 if you have any questions.
Sincerely,
James Warren, ORC
TOWN OF CLAYTON
OPERATIONS CENTER
VEHICLE MAINTENANCE
[phone]
ELECTRIC SERVICE
[phone]
WATER RECLAMATION
[phone]
PUBLIC WORKS
[phone]
DWQ DEHNR
Aquatic Toxicity
1617 Mail Service Center
Raleigh, NC 27699-1617
Enclosed please find the fat head minnow toxicity results for the Little Creek Water
Reclamation Facility. The additional sampling is being conducted due to the NPDES
permit renewal.
653 HiEhWay 42^?71'0 Box 879 • aaylo„. Nonb > 9) 553-!530 - Fax
Date:8/2/2007

Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test
Facility: Town of Clayton County: Johnston NPDES # NC0025453
, •- Cl..-
MAIL ORIGINAL TO:
Test Initiation Date/Time 7/24/2007 /4:30 PM Avg Wt/Surv. Control [0.4781
% Survival [100.01
I 0.478 |Hatch Date 7/23/07
100.01 Hatch Time 3 00PM CT
0.4481Avg Wt (mg) [
% Survival [100.01
Avg Wt (mg) [0.4931
% Survival [97.51
Avg Wt (mg) [0.523 |
[% Survival [100.01
Avg Wt (mg) [0.4991
L % Survival [90.01
Avg Wt (mg) [0.4961
DWQ Form A 7-5 (1/04)
1
7/23/2007
Avg Wt (mg) [
% Survival [
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
8| Surviving #
Original #
Wt/original (mg)
4| Surviving #
Original #
Wt/original (mg)
11 Surviving #
Onginal U
Wt/original (mg)
0.51 Surviving #
Original #
Wt/original (mg)
Cultured In-House
Outside Supplier
Dilution H2O
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt (‘C)
2| Surviving #
Original #
Wt/original (mg)
High Concentration
pH (SU) InIVFin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Growth
Fl
Fl
Normal
Hom. Var.
NOEC
LOEC
ChV
Method
Survival
n n
Test Organisms
r
F
Pipe #: 001
Comments |
8,06
7,25
/ 25.0
8,05
7.56
25.0
8 18
7.60
25.0
batch 162
42
62
188
22.00
48.00
82.00
513
<0,1
1.2
9
10
0.492
10
10
0.542
9
10
0.572
10
10
0.438
10
10
0.483
0______
/ 7,84
/ 7,26
/ 25.0
811
7,77
24.7
batch 163
42
63
225
9
10
0.488
10
10
0.478
10
10
0.516
10
10
0.479
10
10
0.504
2
10
10
0.497
7.84
7.19
25.0
8,13
7.86
24.9
8.27
7,75
24.9
batch 164
42
61
221
8
10
0.532
10
10
0.516
10
10
0.459
10
10
0.468
10
10
0.441
10
10
0.470
10
10
0.546
10
10
0.460
10
10
0.508
10
10
0.409
4
10
10
0.483
8.25
7,60
24.8
Survival
Critical
10
10
10
10
10
8.07
7,38
24.9
8.14
7,33
24.9
8,20
7.52
24.8
6______
/ 8.02
/ 7,49
I 25.0
10
10
0.515
2
7/24/2007
24,00
52.00
85.00
526
<0.1
1.2
3
7/26/2007
3
10
10
0.447
2______
/ 8,09
/ 7,58
I 25.2
Stats
Cone.
0.5
1
2
4
8
Calculated
18
18
16
18
12
8.18
7.57
24.8
Critical
2.41
2.41
2.41
2.41
2.41
24,50
52.00
145.00
8.03
<0.1
1.7
8
>8
>8
Steel's
e
>8
>8
Dunnet's
1______
/ 7,87
/ 7,27
/ 25.0
___£
8.09 /
7.72 /
24.7 /
7. Eff. Repl.
[Control | Surviving #
Original #
Wt/original (mg)
Overall Result
ChV [ >8
0______
/ 8.03
/ 7.53
I 25.0
4______
/ 7,94
/ 6.91
I 25.0
2______
/ 7,97
/ 7,33
I 25.2
3
8.26 / 7,90
7.79 / 7.05
24.8 / 25.0
6______
/ 8.04
/ 7,40
I 25.0
Growth
Calculated
0 5461
-0 1092
-1 4052
0.6262
0.2403
5______
I 8.04
/ 7,17
/ 25.2
5______
/ 8.01
/ 7.21
I 25.2
4
8.07 / 7,91
7.60 / 6,92
24.8 / 25.0
Laboratory Merit^ech, Inc.
x ---------- -------------------
^gn^ture of Operator in Responsible Charge
x___________________________________
Signature of Laboratory Supervisor
Day
______3
8.24 /
7.72 /
24.8
Time: # of Organisms per Chamber: 10Client:
Time:End: Date:Test Vessel Size: 400 ml
Test Solution Volume: 250 mlInitiated by:
°c NoRandomization:esTemp, of Stock:
uT Incubator#: # of Reps: 4
7
Fed by:Transferred by:
InitialTime 3InitialsTime 2InitialsTime 1TimeInitials
Ml Day 0Day 1
Day 1Day 2
Hl (_Day 2Day 3
Day 3
Day 4Day 5
7Day 5Day 6
7,0Day 6
Test Termination Data:
*
= Weekend, only 2 feedings needed
Initials/Signature:
End Date:
End Time:
m1'
NPDES#: NC
Date/Time Fed: 34
Date/Time Born: 7/z-3/o p
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
yfo- z
fallen
2',.
Date
7A<
4 '■ 30
3:3DjC)/(\
2'. | S
Date
Zk.
7/z<r
Vz,
')fa> I
--DajML 7/zy
Start: Date: 7/Wd7
Vs/ A y
7/ A
Client:
I Remarks
D.O.
Temp.
Conductivity
I
Remarks
D.O.
Temp.
Conductivity
Remarks
I
D.O.
Temp.
Conductivity
6 7 Remarks
Concentration: fl.oz
pH_______Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
Concentration: 100%
PH
___________Conductivity
______Residual Chlorine
_____________Hardness
Alkalinity
Concentration: 'Z.Q /
pH______Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
Concentration: V. c> z
pH_______Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
2(y\
zo.|
i
q. 53
7,^-2^
2.5.0
2.^0
2 nr
zo.l
3
7.</b
3
6.OB
5Z
Z1L
AO. I
Day
4
-7.^0
7.7^
7,0 S^
z-W.g'
2^
7? 7
Zd>.|
5
7.^
7,(qO
7.lQ
ZS'.O
Day
4
8^7
7,97
7,(G>
2$.O
7.82
2MX
ZS.O
23^
zo. /
5
7,q2^-7.^z-
7^
-7.il
750
AO.)
Day
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ENVIRONMENTAL LABORATORIES
T»ef.r.olou.y »r>el Cun"O'^ /.
Day
4
57.7
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Test Organism: Pimephales promelas
2
S.l)
7.^'1
7.7?
7.21
24.?
223
7-33
a07
■--------r
2 ;r
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2^
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6
7'0
8.0^
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7J7
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7^.2.
2H(a
2d>!)
Chemical and Physical Determinations Page 2 of 2
NPDE&#: /up poz^^Test Dates: Start: %4z_ End:t/.'30^
Anhlyst(s): Test Tinies: Start: End: 2^ 12^^
---------- -------------——--------------— --------------------------------
INC.
I Concentration: control 6 7 Remarks
pH
D.O.
Temp.
Conductivity
I Remarks
D.O.
Temp.
Conductivity
I
Remarks
D.O.
I Temp.
Conductivity
I
Concentration:
pH
Batch #
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
_______Hardness
Alkalinity
____7
Sdk.
Ro?
Concentration: /,f) */.
pH_________ Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
__6
7 .S'?
Day
4
6<2-(o
Day
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7</l
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Initial
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Initial
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1
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8.03
7.73
7.33
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7,67
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MERSTECH,
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls. Inc.
I
Chemidal and Physical Determinations
Client: C.\qu4T>^________ NPDpS#: me OQ25953 Start Date: 7/^y /o7
Test Organism: 'Fimephaies promelas Analyst(s): fiEnf- End Date: yzi/o?
£20
-Lol
7.5^-
7.^0
25.0
232.
^.o •
___1__
8,13
^.00
7.5g
7.^/
25.0
ZS'O
7oH
2/2,
<o. 1
Time:
Time: Z1.
1
1
/G^
fi.ia
7.53
25 Q
2^0
M-
207
£Qj
6^
Ahalyst(s):
___ _
I
2
Start Date: Client:
End Time: '2.\End Date:
Concentration
Day 5 Day 6 i Day 7Day 3 Day 4Day 0 Day 1
O OO o oRep #1
o (Do ooc>Rep #2
0oooo oRep #3I oo oo 0Rep #4
O>5~ ZConcentration
Day 0 Day 1 Day 3 Day 4 Day 5 Day 6 ; Day 7
o oRep #1 O 0
oo O ORep #2 O
o o o Rep #3 Oc>
0 0oRep #4 O Oo o
I 1.0 7.Concentration
Day 0 Day 1 Day 3 Day 4 Day 5 Day 6 Day 7
0 oRep #1 O d
0Rep #2 o o
I o0Rep #3 O 0o
0 o0 o oRep #4 o 0I
I
I
I
Day 2
I
i—
o
i
i
[}ay2
lyicrxi i cun, iimo.
Mortality Data: Chronic Fathead Test
I
Client:Start Date:
End Time:End Date:
Concentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
/ooooRep #1 O
ooooRep #2 o o
0OooRep #3 OI o
i 00O0Rep #4 o o
i
^.0 7.Concentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 ' Day 7
ORep #1 O . oo_O o
oRep #2 o o o ’ oo
0Rep #3 0 oo oo
0oRep #4 __0_0o n
I R,0 zConcentration
I Day 0 Day 1 Day 3 Day 4 Day 5 Day 6
oRep #1 0 o o o
ORep #2 oo o o
oRep #3 o c>o
0Rep #4 o oO Q
= l
l
I
■I
I
I I ! .
[pay 2
Start Time:
Day 7
I n~
i;
lyitKIlbUH, INU.
Mortality Data: Chronic Fathead Test
I
organism:_Pimephafes promelasI
CONC REP
# ORG.SURV %
A (0 0.^3
B /oo
c IO tj-47 loo
D /o DD
W.A
00
BI A ^4L2
7U.3c 0 100
D to o.9^?/0^
fr&S,co A
ID 0S7T loo1.0 7.I B ^4770
c >0
0 {DO
i
7.^7
7 E3
PAN WT.
<mg)WT. OF ORG.
____(mg)
MEAN
WT./ORG. (mg)
0^2
( 7j-a-
7‘iy.us
-77/
5/6> 7
rw./e- Im./c
I
& t y?-
_________
o n:
Page: \ of 2
Outfall: 001
O
AJ
T
o
L-
i
SS\0CI
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
VDXJU
y: 7^-
l'^.— byc/Ol
PAN + ORG.
WT. (mg)
Facility:
NPDES #: opisy n
Date of Test:
Initial weights taken
Final weights taken on:
1 D
- “SERVICE”“ENVIRONMENT"
I
December 20, 2006
I
Re: Quarterly Toxicity Results
To Whom It May Concern:
Please call me directly at 919-553-1536 if you have any questions.
Sincerely,
James Warren, ORC
I
TOWN OF CLAYTON
OPERATIONS CENTER
Enclosed please find the quarterly toxicity results for the Little Creek Water Reclamation
Facility.
VEHICLE MAINTENANCE
[phone]
ELECTRIC SERVICE
[phone]
WATER RECLAMATION
[phone]
PUBLIC WORKS
[phone]
DWQ DEHNR
Aquatic Toxicity
1617 Mail Service Center
Raleigh, NC 27699-1617
na 27520 • [phone] • Fax (91^53^7
653 • P.O. Box 879 ■
I
I Labora
Comments:I
* PASSED:-1.08% Reduction *
Work Order:
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
I # Young Produced Avg.Reprod.22 22 16 26 22 23 26 26 26 20 23 25
Adult (L)ive (D)ead L L L L L L L L L L L L
1 2 3 4 5 6 7 8 9 10 11 12
PASS FAIL
# Young Produced 20 27 22 18 23 25 22 24 20 27 25 27 X
Adult (L)ive (D)ead Check OneLLLLLLLLLLLL
PH TestControl7.85 7.90 8.01
Treatment 2 7.72 8.03 8.07 8.06 8.02 8.06
1stGrabComp.Duration
Sample 1 X 24 hrs
Sample 2 X
7.02 8.08 427.73
Treatment 2 7.84 6.77 8.29 8.23 8.10 1627.68 776 862
<0.1 <0 .]Test
0.6 2.1as %,
%%%%%%%%
%%%%%%%%
PH D.O.
%%
0.00
Treatment 2
High
Cone.
D
I
L
U
T
23.33
Treatment 2
23.08
Control
S
A
M
P
S
A
M
P
e
n
d
Probit
Other
Control CV
13.122%
0.00
Control
2nd
P/F8
t
a
r
t
1st
e
n
d
s
t
a
r
t
1st
e
n
d
% control
producing
brood
100%
Concentration
Mortality
start/end
Ceriodaphnia dubia
AT-l (3/87)
Organism Tested:
Copied from DWQ form
sample
8.00
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Facility: TOWN OF CLAYTON
1st sample
7.95
1st sample
7.96
2nd sample
7.96
[Sample
combining replicates) ~
%
orgs
3rd
D.o.
Control
Effluent %: 2.0%
TREATMENT 2 ORGANISMS
Duration(hrs):
rev. 11/95 (DUBIA
LC50/Acute Toxicity
(Mortality expressed
%
24.2 hrs
Hardness(mg/1)
Spec. Cond.(pmhos)
Chlorine(mg/1) j
temp, at receipt(°C) •
s
t
a
r
t
2nd sample
8.15
Date: 12/15/06
NPDES#: NC0025453 Pipe#: 001 County: JOHNSTON
Performing Test: MERITECH LABS, INC.
sample
8.26
1 start/end
Control
Mnv?n??d °f Determination
Moving Average
Spearman Karber —
Complete This For Either
Test Start Date: 12/06/06
Collection (Start) ~
Sample 1: 12/04/06 .
Sample Type/Duration
I
Note: Please
Complete This
Section Also
LC50 =
95% Confidence Limits
------% -- %
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
% Mortality
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Chronic Test Results
Calculated t = -0.202
Tabular t = 2.508
% Reduction = -1.08
Date
Sample 2: 12/07/06
ver. 4.41)
X ~ _________
^dgn^ture of Operator in Responsibly Charge ,,
X__-------------------.Z' '' C--
Signature of Laboratory Supervisor
Fed
Grab
Hardness (mg/1)
Total Produced
pH
10 11 Control
O O Treatment 2
D.O.
Control
Treatment 2
Temp.
L L L Control
I Total Produced Treatment 2
Comments: Start End
1
O
4
O
11
o
L
11
12
O
2
o
5
O
L
5
6
O
L
6
7
O
L
7
8
o
L_
8
9
O
L
9
6
O
L
6
7
_O
l|L
7 8
TWTHT
5
o
L
5
O
I
h2h2> 11P, I Ito I2712^| ^71
9
O
8
O
2^ Sai
Client:
NPDES
Dilution Water: Lake Reidsville
Test Organism Source: -fl ~|
Stirred / Aerated for P.O.: Y/i
Control Organism Reproduction
Day #3
# Young Produced
Adults Live / Dead
Day #5___________
# Young Produced
Adults Live / Dead
Day #7
# Young Produced
Adults Live / Dead
^2-
t-O.I
2A
^\z^\
: too %
2nd Sample
25.0125 J
laSanyk •
?. 7q^.o3
1s1 Sample
7.W
Treatment 2 Organism Reproduction
Effluent %:
# Young Produced
Adults Live / Dead
Day #5___________
# Young Produced
Adults Live / Dead
Day #7
# Young Produced
Adults Live / Dead
de
7.9Gl8.ol
S-oalg.oG
2nd Sample
6.15
8.to IW
Sample Type / Duration
100%
pH
272.
• la Sample
2?4> q.qo
^7
. Comp
.'xZ
jo
|3i
10.11
10
o
_L.
10 11 12
9 10
Data Sheet for Mini Chronic Pass / Fail Test:
6 ___7
3 4
L_
4
2-
Time / Date Neonates B-
Age of Nefui;
N y1 Randomized: (Y//N
Sample 1
Sample 2
1* Sample
nJ,?
^.0
Start End
2 3
o Io
T| L| L
12 3 4
-i |3/3 3
i—r-^ 1 ? J 5 6 7 8 9 10 Ti 12
LI C| Lprpj-pqY| l| L Lpr
Llp|2GkL \^\>lo\2.C |t6|zof
Percent Control Producing 3rd Brood:
8.0C
ln Sample
6. OR,
1” Sample
2^ 2^0
Start End
Incubator #:
Date End:
Time End: V • Y
Time;
Time:J?; 09^
Spec. Cond. (umhos/cm) 1/42-1726
Chlorine (mg/l)/
Sample Temp, at Receipt
2^
Day5 Hyt-
Day 6 ML
Terminated by:
1
o
~L| l|l
1 2 3
[l |l |l |l
1 2 3 4 5
|%T
Lit L L It
Ceriodaphnia dubia
---------------------------Pipe#: County:
Time / Date of Culture Transfer:/3>A
- 3om:7A^- iq^,-
tales at Test Start: z <g>,
Culture Tray Temp: 2^ °C
Duration
y
Day2 ^\(L. IflL
. Day 3
Day 4
Date S tan: ^!u{c\a
a^OtJime Start:
____1st Renewal Date:
_____2nd Renewal Date: t^/ti /c^
Analyst:y7fi--^yY)^yyy^ j^evjewef|
Collection (Starfl Dates:
Sample 1: Sample?: Q(o
Test Transferred
By: By:
DayO^/L- H/E
Day 1
12
i
128 9
gA|7/o
I
Zip:
Type of Facility Generating Effluent: LV u? ~r p
I
Test Required:
I

Shipping Information
I
Circle One
I
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:
Client Information
Client:
Contact:
Address:
City:
County:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
Test Organism: Ceriodaphnia Dubia: %
Pimephales promelas:_
Mysidopsis bahia: IWC:
Toxicity Test Concentration(s): _L
List Any Special Requirements:
'PM
. _____. PM
Time: AM(fKJ)
AM PM
Time: //./^
Time: (
Date:
Samp 1 e Condition:
START
END
Sample Chilled {Covered with Ice): Yes
Collector’s Name (Print):
Collector’s Signature:
i
Relinquished By:
Received By:
Relinquished^^:
Received By:
Sample Temperature (C°):
Chronic: 'pf p
Acute:
Sample Receiving (Laboratory ;Use Only)
Received From:
Received By:
Sample Temperature (C°): q. £/Time: AMMT)
PO#
NPDES #: Aid. 002^^^^
Phone: fy (^) ^5’^aC
Pipe Number: p c> f
A y
# of Containers
O 9 PM
ZETbate:
- Date:
Date: A Z
Date: Time:~a;,;
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
ix9<yr,r~<2-h___________________
T.O . gox 95 / /ggg>
*j~b k *\ 5 tn___ State:
p f-f I
Grab Composite
Date: y- 2<aoa> Time:
Date: Time:
No_____
//or 'G''lsS- uCtzb'
Toxicity Test Information
Zip:
s 77"
I P/Z
f ----
Shipping Information
Samn1/» T«t;I ' /—T\ .
Chronic:
Acute:
Composite
Time:
Time:
No__
TJtz
State:
Type of Facility Generating Effluent:
— - I
Sample Informatio
Sampling Site: _
Sample Type:
Sampling Time:
Relinquished By:
Received By:
Relinquished ByST^T"
Received By: -
Sample Temperature (C°):
# of Containers
—pm
——
V 1-----------
Da'e: JaJtfrb
n
______ _ Grab_
Date:
Date:
Meritech, Inc.
B.oassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
IWC:

Toxicity Test Concentrators)-
List Any Special Requirements:'
PO#
p TOES
P^one- f<y if) t, Pipe NumX ooT-
——
z" c

F-0 sy
i ae« “s
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas: ~
Mysidopsis bahia:
Client Information
Client:
Contact:
Address:
City:
County:
STA RT Date:
END Date- T, / >
Sample Chilled (Covered With Ice)-,
Collector s Name (Print)-. —
Collector’s Signature:
Toxicity Test Information
Test Required:
^^zrf oate: Time.
Eate:Time:

Date.' Time:
ztz
Sample Receiving
Received From:~
Received By:
EQUAL VARIANCE t TEST
23.1 23.3
t == -0.202
1.236
Degrees of freedom = 22
Critical t = 2.508
-0.202 < 2.508
I
Chronic Test PASSES
1
I
I
Test passed. There is not a significant difference in reproduction
between the Control and the effluent evaluated at a 99% confidence interval.
I
“SERVICE”“ENVIRONMENT"
I
I

March 19, 2007
I
Re: Quarterly Toxicity Results
I To Whom It May Concern:
Please call me directly at 919-553-1536 if you have any questions.
Sincerely,
James Warren, ORC
I
I
VEHICLE MAINTENANCE
[phone]
ELECTRIC SERVICE
[phone]
WATER RECLAMATION
[phone]
PUBLIC WORKS
[phone]
TOWN OF CLAYTON
OPERA TIONS CENTER
DWQ DEHNR
Aquatic Toxicity
1617 Mail Service Center
Raleigh, NC 27699-1617
Enclosed please find the quarterly toxicity results for the Little Creek Water Reclamation
Facility.
Date: 03/14/07
001 County: JOHNSTON
Comments:
* PASSED:-4.50% Reduction *I Work Order:
MAIL ORIGINAL TO:
I CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
% Mortality Avg.Reprod.# Young Produced 19 27 25 27 28 12 26 28 26 24 19 28
Adult (L)ive (D)ead L L L L L L L L L L L LI
1 2 3 4 5 6 7 8 9 10 11 12
PASS FAIL
# Young Produced 24 23 22 28 30 25 21 29 27 26 23 24 X
Adult (L)ive (D)ead Check OneLLLLLLLLLLLL
pH TestControlI7.99 8.03 7.98
Treatment 2 7.96 7.93 7.96 8.02 8.02 7.97
1stIGrabComp.Duration
Sample 1 X 24.2 hrs
Sample 2 X
7.99 7.98 407.83
7.87 8.19 8.10 1668.20 6367.86 667
<0.1 <0.1
1.2as %,
%%%%%%%%Concentration%%%%%%%%%%
PH D.O.
I
combining replicates)
%
7.94
Probit
Other
orgs
3rd
D
I
L
U
T
24.08
Control
S
A
M
P
S
A
M
P
s
t
a
r
t
1st
0.00
Treatment 2
Control CV
20.530%
0.00
Control
High
Cone.
25.17
Treatment 2
2nd
P/Fe
n
d
e
n
d
e
n
d
R9sponsible Charge
Mortality
start/end
Ceriodap hnia dubia
AT-l (3/87)
sample
7.95
2nd sample
8.03
1st sample
8.05
1st sample
8.02
Effluent %: 2.0%
TREATMENT 2 ORGANISMS
LC50/Acute Toxicity Test
(Mortality expressed
%
s
t
a
t
2nd sample
8.22
% control
producing
brood
91.7%
s
t
a
t
1st sample
8.25
Duration(hrs):
rev. 11/95 (DUBIA
, 1'2
I
Note: Please
Complete This
Section Also
of Determination Moving Average
Spearman Karber —
D.O.
Control
1 start/end
Control
| Organism Tested:
Copied from DWQ form
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Chronic Test Results
Calculated t = -0.657
Tabular t = 2.508
% Reduction = -4.50
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
[Facility: TOWN OF CLAYTON NPDES#: NC0025453 Pipe#:
jaboratory^erform^^^st; MERITECH LABS, INC.
Signat^r^of O^r:a$q£, -----J
Signature of Laboratory Supervisor
Test
Collection (Start) “
Sample 1: 03/06/07 .
Sample Type/Duration
Date
Sample 2: 03/08/07
24.8 hrs
Hardness(mg/1)
Spec. Cond. (jzmhos)
Chlorine(mg/1) •
Sample temp, at receipt(°C) :
ver. 4.41)
LC50 = %
95% Confidence Limits
------% %
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
I
Treatment 2
Grab
Total Produced
pH I8* Sa;le 2nd Sample
11 Control
&Treatment 2
D.O.
Control
Treatment 2
Temp.
L ControlL-
Treatment 21^ |27 la/pl^g 1^Total Produced
Comment*-
ilia]
iflial
11
o
3 ;
5
O
L
5
8
O
L
9
O
9
O
2
2
O
U
2
L~
5
6
o
6
3 H
u
6 7
9
12
~1
1
C>
1
13
L;
1
12
O~c
7
O
u
7
8
O
u
8
10
L- U|
10 11
V I y|9
L-
Hardness (mg/1)
Spec. Cond. (umhos/cm)
Chlorine (mg/1)
Sample Temp, at Receipt
Sample 1
Sample 2
2
O
L_
2
5
O
u
5
z
L
5
12
O
12
6
O
u
6
2
U
6
J_
O
1
T
gl40.l
6, GT
<0. |
7
O
U-
7
ZXs
7
Sample Type / Duration
100%
pH
'7.75'
8.22 7.83
8.20 H, fit.
8.03 778
g. cal 1.77
2nd Sample
Duration
2^.2
Comp
X
*
£
4
1 pH
U |u
2 3/4
-l > J41 3 6 .Z - ■.8 - 9_, 10. U 12
I I U |L | l- L L i L L L
7/6,1,13
1 * Sample
7.7^
7.8? |q.q4
1“ Sample
-z^0 24.1
124.1
Start End
AAO
Day5
Day 6
Terminated by:
3 I 4
oi
u
3 ;
10
O Tl| c
8 9 10 11
L-I Y t | L
J-E-i 2; I V, 4 , , 5, , 6 , 7 f . 8 9 10. 11, 12
^/al % |^| 7/$ |/^,
I | ^''1 L I L. I I 1- | I | L L, u
>ollr^r&A>. Date Start: 2>/7/0
Time Start: 7-3Of^
-/ lst Renewal Date: Zlgloy
_2nd Renewal Date: 3/z/g?
Analyst:A)^/^L/^(t
Collection (Start) Dates:
Sample 1:
2nd Sample
25.1 24.9
25.o|24.9
Start End
1M Sample
8.02 8.Q3
'l.uls.cgi
1 * Sample
8,25 1.9ft
8.1918.10
I11 Sample
25./ 250_
25. Z| 25.0
Start End
4
_O
_L
3 I 4
Ml z
~u| L
3 ! 4
j 1T1C1 ICCC11
Data Sheet for Mini Chronic Pass / Fail Test: Ceriodaphnia dubia
Pipe #:
-------------.j Time / Date Neonates Bom: 7~ A
.ates at Test Start:
Culture Tray Temp: 2^°C
NPDES ^>0^5-5/ Time / Date of Cdture Transfer
Dilution Water: Lake Reidsville Time / Date Neonates Bom: 7 - -
Test Organism Source. ! Age of NeM?ipt? t #»ct q
Stirred / Aerated for P.O.: Y / Randomized: N
Control Organism Reproduction 1
Day #3__________
# Young Produced
Adults Live / Dead
Day #5_________
# Young Produced
Adults Live / Dead
Day #7__________
# Young Produced
Adults Live / Dead
. . 1^.1^
Percent Control Producing 3rd Brood: 7 °/0
Treatment 2 Organism Reproduction
Effluent %:
# Young Produced
Adults Live / Dead
Day #5
# Young Produced
Adults Live / Dead
Day #7__________
# Young Produced
Adults Live / Dead
Incubator #:
_ Date End: ^//1^/07
Time Endjat/r^
Time: :CO/\r^
Time:
Reviewed by: ^0—
Sample 2: 3 /
Test Transferred Fed
By: By:
DayO
Day 1 Mt
Day 2 (AC
Day 3
Day 4
I
I 030'11^/
I
I
State: Zip: I Type of Facility Generating Effluent:S Lj “F
I
I
I
I Toxicity Test Information
Test Required:
I P/A c>
:y:
I
Sample Information
Sampling Site:
Sample Type:
Sampling Time:
Chronic:
Acute:
START
END
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
Time: AM^?
------cv.
Shipping Information
Relinquished By: *
Received By:
Relinquished
Received By:
Sample Temperature (C°):
'omposite
3'6 ~^oc*} Time:
B - 7 '2.oe>^i Time:
X No
Date: S/l/o')
Sample Condition:
# of Containers
& PM
PM
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements:
Time: /.»__
Time: AM^
Time: X£zT~AMjjvt
Time: AM PM
UPS Fed-Ex—Circle One
Sample Receiving (Laboratory Use Only)
Received From: SC f
Received By:(/XQ^Ol/
Sample Temperature (C°):/L^// 2~
Date: >e>>
Date: 7- z—^>-7
Date: 3-7 "^7
Date:___
Method of Shipment: WTC(p/u)
Circle One Other:
X Grab
Date: _
Date: _
Sample Chilled (Covered with Ice): Yes
Collector’s Name (Print): J
Collector’s Signature:
PO#_
NPDES #:
Phone:
Pipe Number: p o y
Client Information
Client: q ______
Contact: _____________
Address: - n? Z7^ Dccrk^
City: (f ('/■'o m
County: n
I
I
IState:
Type of Facility Generating Effluent:O’ r'a.ci-e,
I
I
Toxicity Test Information
Test Required:
C o c-.,
I
Shipping Information
^71I
Circle One
Sample Information
Sampling Site:
Sample Type:
Sampling Time:
Chronic:
Acute:
START
END
Collector’s Name (Print)-.
Collector’s Signature:
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
# of Containers_a
@J)PM
.<©) PM
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
Date: ]
SampleConditiom
IWC: ______
Toxicity Test Concentration(s):
List Any Special Requirements: '
CP 9 (/S'
*V1 p
A ri
Received From:
Received By: ------
Sample Temperature (C°): V ■
7
Sample Receiving (Laboratory Use Only)
Composite
Time:
Time:
No__
//d r.j
sty$
Time: AM pM
Time: AM PM
Time: J'C/p AM fjQ/
Time: AM PM
UPS Fed-Ex
Relinquished By:
Received By:
Relinquished By:
Received By: <
Sample Temperature (C°):
Date: s/f/o 7
Date:
Date: 3/9/ajy
Date:
_ Method of Shipment: WTC(p/u)
Circle One Other:
PO^______________
NPDES #: A/e QP £ SQS.^,
Phone: Lsif) SSS -/
Pipe Number:
AJ Zip: ^-7
Ti^me: AM
-Iced
Client Information
Client: o-f-
Contact:
Address: p. (^.gpy //f q y y
City; 6/ey^/n
County:
Grab__
Date: _
Date:
Sample Chilled (Covered with Ice): Yes
I
Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 03/14/07IFacility: TOWN OF CLAYTON Pipe#: 001 County:JOHNS':
I INC.
Reduction:-4.50%CONTROL 2.0% Effluent
# Replicates 12 12
Female Live 12 12
I Adult Male 0 0
Adult Dead 0 0
0.00%0.00%
289 302
Mean # Neonates 24.083 25.167
Standard Deviation 4.944 2.855
Coefficient of Variation 20.530%
I
Fisher's Exact Test
A = 12 B = 12 b = 12
a/A = 1.00 b/B =1.00
Success is:survival
Critical b value =8
12 > 8
I
I Test Passes!
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA
i Group Neonates •i Group Neonates Centered
I
Adult Mortality
# Neonates
ORDERED OBSERVATIONS
Centered
C
C c
E
E
E
E
E
E
E
C
E
12
19
19
21
22
23
23
24
24
25
24
26
-12.0833
-5.0833
-5.0833
-4.1667
-3.1667
-2.1667
-2.1667
-1.1667
-1.1667
-0.1667
-0.0833
0.8333
C
E
C
C
E
C
C
E
C
c c
E
25
27
26
26
28
27
27
29
28
28
28
30
0.9167
1.8333
1.9167
1.9167
2.8333
2.9167
2.9167
3.8333
3.9167
3.9167
3.9167
4.8333
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
the proportion of t.
for the control and
The test concludes that
significantly different
a = 12
surviyal is not
d the effluent groups.
NPDES#: NC0025453
Laboratory Performing Test: MERITECH LABS,
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.)
COEFFICIENTS AND DIFFERENCES
i x(n-i-1)x(i)a (i)x(n-i-1)x(i)
I
1w X 320.2952
358.5833
Calculated W = 0.893 Critical W = 0.884
0.893 i 0.884
evaluated at 99% confidence interval.
Test Passes!I
F test for Homogeneity of Variance
Control variance 24.4470F == 3.00Effluent variance 8.1515
Numerator degrees of freedom:11 11
3.00 5.32 =► The Test£
significantly theare
I
I
I
PASSES, the variances of the
same, homogeneous.
Denominator degrees of freedom:
Critical F = 5.32
1
2
3
4
5
6
7
8
9
10-
11
12
4.8333
3.9167
3.9167
3.9167
3.8333
2.9167
2.9167
2.8333
1.9167
1.9167
1.8333
0.9167
-12.0833
-5.0833
-5.0833
-4.1667
-3.1667
-2.1667
-2.1667
-1.1667
-1.1667
-0.1667
-0.0833
0.8333
0.4493
0.3098
0.2554
0.2145
0.1807
0.1512
0.1245
0.0997
0.0764
0.0539
0.0321
0.0107
16.9166
9.0000
9.0000
8.0834
7.0000
5.0834
5.0834
4.0000
3.0834
2.0834
1.9166
0.0834
The reproduction data is normally distributed
evaluated at a 99% confidence interval
two groups
EQUAL VARIANCE t TEST
24.1 25.2
t == -0.657
1.648
I Degrees of freedom = 22
Critical t = 2.508I-0.657 < 2.508
I
Chronic Test PASSES
I
I
I
I
Test passed. There is not a significant difference in reproduction
between the Control and the effluent evaluated at a 99% confidence interval.
I
I
5^
State:
Type of Facility Generating Effluent:kJ c.J T p
I
Toxicity Test Information
P/A
I
I
I Sample Receiving (Laboratory Use Only)
Received From:
Time: AM PMI/
Received By:
Sample Temperature (C°):
Sample Information
Sampling Site:
Sample Type:
Sampling Time:START
END
Date:
Sample Condition:
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]

7 - c’y
l:i5 _
' am Em
AM PM
__2 am pm
O 8 'J
X e ,
.Z
-5
/ fl M
ccr A <n<.f
.J-
X Grab
Date:
Date: _
Sample Chilled {Covered with Ice): Yes
Collector’s Name {Print):
Collector’s Signature:
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
Shipping Information
Ldfc.rr e rx
y //Md 7)
__________
t\ I .5 I 6
PO#______________
NPDES #:
Phone: (■>/.-> <5- - z>- ?-
Pipe Number: q /
Zip: 7C<3%
____cP <~f / Z f p / _____
Composite
3 -4 -Joe 7 Time:
J " ? - 7 Time:
> X No
/f c ( ,
Relinquished By:
Received By:
Relinquished By?
Received By:
Sample Temperature (C°):
Client Information
Client: u? M f)
Contact: 5
Address: po
City: (I +e
County:
# of Containers 2_
(@i PM
PM
Date: Time:
Date: 7- ^7 Time:
----------Date:Time:
Date: Time:______
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
Test Required: Chronic:
Acute:
I 7) „
State: Zip:
Type of Facility Generating Effluent:l,
E-f-f '4I
I Toxicity Test Information
I

Shipping Information
I
I Sample Receiving (Laboratory Use Only)
Received From:
Time: AM PM/
Received By:
Sample Temperature (C°):
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:START
END
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
Date:____
_ Sample Condition:
Test Required: Chronic:
Acute:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]

@pPM
PM
PO#_______________
NPDES #: /Vc c^o j < y^-~,
Phone: (5/,) 3C
o a i
JJt,
rTc-1
# of Containers
O9o o
O f a (cc,-/
C~ r c--. C1- e
C an .
________
Composite
Time:
Time:
No
IWC: _________
Toxicity Test Concentration(s): p//^
List Any Special Requirements:
Relinquished By:
Received By:
Relinquished By:
Received By:'
Sample Temperature (C°):
Time: AM PM
Time: AM PM
Time:AM PM
Time:AM PM
C.) n ts ' 1
3 h
Pipe Number:
7-5^ <3
Client Information
Client: /•? or tx / ?
Contact: 5
Address: p c? • F p y ? q o o
City:
County:
3^ Date: 3 7
_ Date: 3/
Date:_______
Date:
Method of Shipment: WTC(p/u) -UPS Fed-Ex
Circle One Other:
_ Grab _
Date: j - g - c >
Date: J -f-o?
Sample Chilled (Covered with Ice): Yes
Collector’s Name (Print): ~
Collector’s Signature:
-“SERVICE”“ENVIRONMENT”
I
July 9, 2007
Re: Quarterly Toxicity Results
To Whom It May Concern:
Please call me directly at 919-553-1536 if you have any questions.
I
VEHICLE MAINTENANCE
[phone]
ELECTRIC SERVICE
[phone]
WATER RECLAMATION
[phone]
PUBLIC WORKS
[phone]
TOWN OF CLAYTON
OPERATIONS CENTER
DWQ DEHNR
Aquatic Toxicity
1617 Mail Service Center
Raleigh, NC 27699-1617
Sincerely,
^J3mes Warren, ORC
653 Highway 42 West • P.O. Box 879 • Ciayton, North Cawi.na 27520 . (9I9> 553.153O . Fax (9]9) 553.,541
Enclosed please find the quarterly toxicity results for the Little Creek Water Reclamation
Facility.
ON
Date: 06/28/07
I NPDES#: NC0025453 Pipe#:001 County: JOHNSTON
Laboratory orming 'est: MERITECH,INC
Comments:
re
* PASSED:-2.93% Reduction *
'Work Order:
MAIL ORIGINAL TO:
1 2 3 4 5 6 7 8 9 10 11 12
% Mortality Avg.Reprod.# Young Produced 23 23 26 26 29 25 25 23 25 22 31 29
L L L L L L L L L L L L
1 2 3 4 5 6 7 8 9 10 11 12
PASS FAIL
# Young Produced 24 23 25 25 30 28 25 26 29 27 29 25 X
Check OneAdult (Dive (D) ead L L L L L L L L L L L L
1st sample 1st sample 2nd sample
Control 8.03 8.01 8.08 8.07 8.12 7.99
8.03 8.05 8.10 8.11 8.10 8.03
1stGrabComp.Duration
Sample 1 X 23.5 hrs
Sample 2 X 2 5 hrs
Control 7.85 7.44 427.56 7.25 7.62 7.33
7.78 7.49 183 7647.60 7807.33 7.68 7.37
<0.1 <0.1
1.0 1.0
%%%%%%%%Concentration
%%%%%%%%%%
start/end
Control
pHD.O.
Organism Tested:
I
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Facility: TOWN OF CLAYTON
Probit
Other
Hardness(mg/1)
Spec. Cond. (jzmhos)
High
Cone .
I
Note: Please
Complete This
Section Also
D
I
L
U
T
S
A
M
P
s
t
a
0.00
Treatment 2
Control CV
10.987%
0.00
Control
26.33
Treatment 2
25.58
Control
2nd
P/F
S
A
M
P
e
n
d
e
n
d
e
n
d
'C5 0 = ______%
95% Confidence Limits
% -- %
I
Treatment 2
1
combining replicates)
%
Mortality
start/end
pH
treatment 2
icONTROL ORGANISMS
t
1st sample
s
t
a
r
t
1st sample
s
t
a
r
t
2nd sample
|| organism Tested: Ceriodaphnia dubia
Bpopied from DWQ form AT-1 (3/87)
Chronic Test Results
Calculated t = -0.724
Tabular t = 2.508
% Reduction = -2.93
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
f Operator in Responsible ,Charge
% control orgs
producing 3rd
brood
100%
Method of Determination
Moving Average
Spearman Karber
X __________ ___________ . , . -
Signature of Laboratory Supervisor
X
Signa
Adult (Dive (D) ead
■Effluent %: 2.0%
TREATMENT 2 ORGANISMS
'LC50/Acute Toxicity Test
Mortality expressed as %,
%
Chlorine(mg/1)
Sample temp, at receipt(°C)
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
Complete This For Either Test
Test Start Date: 06/06/07
Collection (Start) Date
Sample 1: 06/05/07 Sample 2: 06/07/07
Sample Type/Duration
Duration(hrs):
rev. 11/95 (DUBIAver. 4.41)
Total Produced
pH
Control 'S’.ol
Control I
Treatment 2
Temp.
Control
Treatment 2
nir
1
o
4
8
O £
8
7
O
U
7
8
10
O
L
10
11
o
11
2
o
5
o
6
O
1
o
8
O
-77 I 2/ ■ 3 4 5
±J
u
10
Z6,/ rr
L
8
u
1
100’/.
pH
L
2
4
O
L
4
5_
O
__LT
12345SBraoznz ' ‘ L. '
L i u u
6
O
IT
6
J- L
_6_
9 10 11 12
u I L cl I L.
3rd Brood:/7YJ%
3
O
u
3
Control Organism Reproduction
~ , "3 ~£2
o
L
Total Produced
Comments:
Percent Confrol Producing 3 rd
2
o
L
Sample 1
Sample 2
3
o
1 2 3
aTTTT
I
4
O
u
4
U-
L
9
o
T
9
L
7
It""
||pf ^-O. I
Hilo’
Treatment 2
D.O.
P* Sample
y-QS uzd
1M Sample
10
O u
10
5.
11
o
L
11
S 3
12
c
L
12
11
12
o
T
12
5
L
L L
5 6
-231a
*25.0
Start End
9
O
LJ L | L | L~TC
6 7 8 9
i—rrr
7 ' ■ 8 ■ , 9 ; , 10, JI. 12
^TlJjUjZIIZITjX
Hardness (mg/1)
Spec. Cond. (umhos/cm)
Chlorine (mg/1)
Sample Temp, at Receipt
I
1st Sample
2^25£
zF/|2Si
Start End
2nd Sample
dd- Sjolg.oj
2nd Sample
Itwwi
2nd Sample
^■0 25:0
Start End
1st Sample
^03
1“ Sample-----------—j-E-------- * oampic
P1 Sample
Date Start:j
Time Start:___C
1 st Renewal Date?
2nd Renewal Date: «
AnalystryHL^^gR^
Collection (Start) Dates:
Sample 1:
Sample Type / Duration ;
Grab I Comp Duration I
JZJHZJ
Treatment 2 Organism Reproduction
Effluent %T
Z.O
# Young Produced
Adults Live / Dead
Day #5 x
# Young Produced
Adults Live / Dead
Day #7
# Young Produced
Adults Live / Dead
H^S'py-
Reviewed by:
Incubator #: 7)
— Date End:
, Time End:~
Time: g
Time.-TVTg^T-
Meritech
Data Sheet for Mtn, Chronic Pass / Fail Test: Ceriodaphnia dubia
Client: /^\ri
NPDES#: -----r-----7n'Pe County-

Test Organism '
CoitroLOrgS^Renr^Z^:--------- -------------— Culture Trav Temp: Z5L°C
Day #3 _______ _
# Young Produced
Adults Live / Dead
~Day #5
# Young Produced
Adults Live / Dead
bay #7
# Young Produced
Adults Live / Dead
Sample 2: 6 /? /> 7
Test Transferred Fed
By: By:
Day 0
Day 1
Day 2 mA-
Day 3
Day4 ^(/L-
Day5(Mj[L
Day 6
Terminated by:
Ql^Ob 1^0
I
I
Collector’s Name (Print):
I

I
I
Id
I
Chronic:
Acute:
WPM
Wpm
i
■y Use Only)
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:

Mysidopsis bahia:
# of Containers
IDiOC
^CLO.______ PO#
PhoDneS(£^^
Coun‘y: ---- P,pe Number:
^ate. Zip: ^75-^ y
Type of Facility Generating Effluent: 77^
Sample Information
Sampling Site: EP-Pj
Sample Type:
Sampling Time:
Time:
Composite
Time:
Time:
No__
u

Shipping Information
Client Information
Client:o-f
Address:
City:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]

Date:
Sample Condition:
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements:'
Sample Receiving (Laborator
Received From: CT /V
Received By:
Sample Temperature (C°): l.QTfTpy
- Grab_ P^ ZCon
S TART Date: C /S'/o 7
END Date:
Sample Chilled (Covered with Ice)-
Collector’s Name (Print): / • J-----5c7~"
Collector’s Signature:
Toxicity Test Information
Test Required:

Received By:^ate- Time:
~ Clrc^ One Other:
I
I
I
Toxicity Test Information
Test Required:
X

I
I
I
i
Chronic:
Acute:
Sample Information
Sampling Site:
Sample Type:
Sampling Time:
Grab
Date:
Date:
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
Shipping Information
of Containers
O PM
_ PM
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
Relinquished By: y /
Received By:^^^TT^
Relinquished
Received By:
Sample Temperature (C°):
IWC:
Toxicity Test Concentration(s)-
List Any Special Requirements:'
_____ PO#
----- NPDES #: f) OA ^Te/<r 3
57- Phone: (fitf)
Pipe Number: e> /
ZiP;
A

Client Information
Client: TflutK of
a j jtaCt’ ~ Ldh.rre n

County: //, K n State: fOc
Type of Facility Generating Effluent:
Sample Receiving (Labora^Tu^O^^
Received From: Qvq^
Received . C c Sample Ten^^^O^ AM®
-y Q-frt p
CompositeSTART Date: PTime:
Sample Chilled (Covered with Icef^^^2- n™6'
Collector’s Name {Print)-.r~
Collector’s Signature:
-i-^Sfoy Time: PM
Method ofSh^i^WTC^yuPS F^dS
______ Circle One Other:
Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 06/28/07
NPDES#: NC0025453 Pipe#: 001 County:JOHNSTON
INC
Reduction:-2.93%CONTROL 2.0% Effluent
# Replicates 12 12
Female Live 12 12
Adult Male 0 0
Adult Dead 0 0
Adult 0.00%0.00%
307 316
Mean # Neonates 25.583 26.333
Standard Deviation 2.811 2.229
Coefficient of Variation 10.987%
Fisher's Exact Test
A = 12 B = 12 a = 12 b = 12
a/A = 1.00 b/B =1.00
Success is:
8
SHAPIRO-WILK1S TEST FOR NORMAL distribution OF DATA
i Group Neonates i Group Neonates Centered
I
I
I
Facility: TOWN OF CLAYTON
Laboratory Performing
ORDERED OBSERVATIONS
Centered
C
E
C
C
C
E
E
E
E
E
C
C
22
23
23
23
23
24
25
25
25
25
25
25
-3.5833
"3.3333
-2.5833
-2.5833
-2.5833
-2.3333
-1.3333
-1.3333
-1.3333
-1.3333
-0.5833
-0.5833
13
14
15
16
17
18
19
20
21
22
23
24
25
26
26
26
27
28
29
29
29
29
30
31
-0.5833
-0.3333
0.4167
0.4167
0.6667
1.6667
2.6667
2.6667
3.4167
3.4167
3.6667
5.4167
1
2
3
4
5
6
7
8
9
10
11
12
C
E
C
C
E
E
E
E
C
C
E
C
Mortality
# Neonates
survival
Critical b value =
12 > 8
Test Passes!
Test: MERITECH,
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.)
COEFFICIENTS AND DIFFERENCES
i x(n-i-1)x(i)a (i)x(n-i-i)x(i)
I
1w X 133.2945
141.5833
Calculated W =0.941 Critical W = 0.884
0.941 a 0.884
evaluated at
Test Passes!
F test for Homogeneity of Variance
Control 7.9015F =
= 1.59
4.9697
Numerator degrees of freedom:11
of freedom:11
1.59
PASSES,of the two groupsare
I
=► The Test
significantly the
the variances
same, homogeneous.
5.4167
3.6667
3.4167
3.4167
2.6667
2.6667
1.6667
0.6667
0.4167
0.4167
-0.3333
-0.5833
-3.5833
-3.3333
-2.5833
-2.5833
-2.5833
-2.3333
-1.3333
-1.3333
-1.3333
-1.3333
-0.5833
-0.5833
0.4493
0.3098
0.2554
0.2145
0.1807
0.1512
0.1245
0.0997
0.0764
0.0539
0.0321
0.0107
9.0000
7.0000
6.0000
6.0000
5.2500
5.0000
3.0000
2.0000
1.7500
1.7500
0.2500
0.0000
1
2
3
4
5
6
7
8
9
10
11
12
variance
Effluent variance
Denominator degrees
Critical F = 5.32
S 5.32
EQUAL VARIANCE t TEST
I 25.6 26.3
t == -0.7241.036
-0.724 < 2.508
There is not
evaluated at
I
Chronic Test PASSES
I
Degrees of freedom = 22
Critical t = 2.508
-“SERVICE”“ENVIRONMENT”
I
July 9, 2007
Re: Quarterly Toxicity Results
To Whom It May Concern:
I
I Please call me directly at 919-553-1536 if you have any questions.
Sincerely,
es Warren, ORC
TOWN OF CLAYTON
OPERATIONS CENTER
VEHICLE MAINTENANCE
[phone]
ELECTRIC SERVICE
[phone]
WATER RECLAMATION
[phone]
PUBLIC WORKS
[phone]
DWQ DEHNR
Aquatic Toxicity
1617 Mail Service Center
Raleigh, NC 27699-1617
Enclosed please find the quarterly toxicity results for the Little Creek Water Reclamation
Facility.
653 Highway 42!
Comments:
* PASSED:I Work Order:
MAIL ORIGINAL TO:
Test
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
% Mortality Avg.Reprod.# Young Produced 23 23 26 26 29 25 25 23 25 22 31 29
Adult (L)ive (D)ead L L L L L L L L L L L L
1 2 3 4 5 6 7 8 9 10 11 12
PASS FAIL
# Young Produced 24 23 25 25 30 28 25 26 29 27 29 25 orgs X
Adult (Dive (D) ead Check OneLLLLLLLLLLLL
1st sample 1st sample 2nd samplepH TestControl8.03 8.01I 8.08 8.07 8.12 7.99
Treatment 2 8.03 Sample 2: 06/07/078.05 8.10 8.11 8.10 8.03
1stGrabComp.Duration
Sample 1 X 23.5 hrs
Sample 2 X 2 5 hrsI7.85 7.44 7.56 427.25 7.62 7.33
Treatment 2 7.78 7.49 7.60 7.33 183 7647.68 7807.37
<0.1 <0.1
Sample temp.1.0 1.0as %,
%%%%%%%%Concentration
%%%%%%%%%%
PH D.O.
Organism Tested:
I
combining replicates)
%
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Facility: TOWN OF CLAYTON
Probit
Other
Control CV
10.987%
High
Cone.
S
A
M
P
S
A
M
P
0.00
Treatment 2
I
Note: Please
Complete This
Section Also
D
I
L
U
T
26.33
Treatment 2
25.58
Control
2nd
P/Fe
n
d
e
n
d
e
n
d
0.00
Control
Mortality
start/end
Duration(hrs):
rev. 11/95 (DUBIA
start/end
Control
D.O.
Control
Effluent %: 2.0%
TREATMENT 2 ORGANISMS
s
t
a
r
t
1st sample
s
t
a
r
t
1st sample
s
t
a
r
t
2nd sample
Ceriodaphnia dubia
Copied from DWQ form AT-1 (3/87)
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
MovinghAve?^geeterminati°n
Spearman Karber —
% control <—~
producing 3rd
brood
100%
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity
LC50 = %
95% Confidence Limits
-------% %
Hardness(mg/1)
Spec. Cond. (/zmhos)
Chlorine(mg/1)
at receipt(°C)
Complete This For Either
Test Start Date: 06/06/07
Collection (Start) Date
Sample 1: 06/05/07 ,
Sample Type/Duration
Chronic Test Results
Calculated t = -0.724
Tabular t = 2.508
% Reduction = -2.93
LC50/Acute Toxicity Test
(Mortality expressed
%
ver. 4.41)
-2.93% Reduction *
Date: 06/28/07
NPDES#: NC0025453 Pipe#: 001 County: JOHNSTON
Laboratory Pexforming Test: MERITECH, INC
X
Signayrire/df Operator in Responsible ,Charg^
Signature of Laboratory Supervisor —
Comp
Total Produced 23
Control
Treatment 2
2^
Total Produced
Treatment 2
comments:
L
2
L.
3
L
4
L
6
Control
2
O
4
o
4
O
L
9
o
11
o
8
o u
7
O
LT
ii
o
L
n
Sample 1
Sample 2
Temp.
Control
u
L
5
_6_
z_
6
O
IT
3
O
L
Duration
Z3z5^
Date Start:
Time Start:
1st Renewal Date// ^^<5-/07
2nd Renewal Date-
2 nd Sample3
o
2
?
o
6
O
J0_
o
_L [lTuXHl
7 8 9 10 11
8
O
L| L
'i 12
223
L
*C
Treatment 2
D.O.
9
O
T
9
10
o
L
10±
L.
10
2
o
L
12
c £
12
/
To-
1
o
_£
1 2 3 4
HTT75H
‘ T |C
3 4
U
_L
-LJjUj- L (_ u (_ u TT7
5
Incubator #:
Date End: <0/,^/^
Time End.7^ •
Time:___e^75^
1M Sample
-fraSg.0'7]
1 * Sample
1st Sample
zH/fisTri
Start End
Data Sheet for
c,ient:—ru+o.
npdes#rrTrm
Dilution Water: Lake Reidsvill
Test Organism Source-
Control Organism
Day #3
# Young Produced
Adults Live / Dead
Day #5
# Young Produced
Adults Live / Dead
Day #7
# Young Produced
Adults Live / Dead
lai L7.6817.3^1
2nd Sample
Start End
5
o
klLj L
5 6 7 8 9
^Tg~FT|FTTr;*T 1 l~i
7 8 9
rQZZETKdTTt7
100%
__pH_
7.55 _
fg.
i
J/2.
I78O
M|To~
Collection (Start) Dates:
Sample 1: 6/570-7
Sample Type / Duration [
Grab
Meritech
Cenodaphnia dubia
--- Pipe #: ppi C._..
Time / Date of Culture Transfe;
3/ |
5
o
TT
5 6 7 8
MSSS
L_
7
L_
Percent Control Producing 3rd Brood:
Treatment 2 Organism Reproduction
Effluent %;
_ 3.0
Young Produced
Adults Live / Dead
Day #5 .
# Young Produced
Adults Live / Dead
Day #7 ~
Young Produced
Adults Live / Dead
Hardness (mg/1)
Spec. Cond. (umhos/cm)
Chlorine (mg/1)
_Sample Temp, at Receipt
_ 1MSanyle
. ^°-3 ig.oi
goT[^o5
1“ Sample
.123
p-76
1“ Sample
^.0
[ITT
Start End
2nd Sample
Mini Chronic Pass / Fail Test:
Time ' D„e Bo- Zto-
------ ASe of Neonates at Te^t^t^q^T^------
Culture Tray Temp: °(Control Organism Reproduction
1
O
L
1 2 3 4
L
J°Ado2_
2ndRGne^Date~^^_
Reviewed by: 3^-
Sample 2:_ h Io-)
Test Transferred Fed
By: By:
Day 0
Day 1
Day2Wflr
Day3 fltf'
Day4 fH/L.
DaySfMjL
Day 6
Terminated by:
I
QigOb 1^0
I
—------- Test Organism:
I A <s>
I
II
I
Time:
§52
!
I
Chronic:
Acute:
!
No
of Containers
-£Z5O_^m)pm
----- ------- ro#_
-^J~-
IWC:
Toxicity T^sTCon^mrationfs);
,st ny Special Requirements:
START
END
Sample Chilled (Covc.^J
Collector’s Name {Print)-.
Sample Condition:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]

Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia: ---------
Date:
Date:
------------ Date:
------- - Method
___________Circle One
y Use Only)
72U
Sample Receiving (Laborator
Received From: jT /V| J fl
Client Information
Client:o-f
e°"tact--
Address: /ooe)
City-_Stfi
County;
Type of Facility Generating Effluent:
Sample Information
Sampling Site:
Sample Type:
Sampling Time:
LZ__ Ti m e:
Time:
T---------AM PM
(P/U) UPS Fed-Ex
Other:
Shipping Information
Relinquished By; y .
Received By:
RelinquishedBy*^^^
Received By:
Sample Temperature (C0)?
<^ab----- Composite
^ate: Time:
Date: £ '
'ered with Ice): Yes

Collector’s Signature:

Test Required:
Received By; Csx/lAA/
Sample Temperature (C0)-
I

I
I
Toxicity Ttest Information

Shipping Information
I
I
Test Required: Chronic:
Acute:
# of Containers__
o PM
Grab _
Date:
Date:
Si!)
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
IWC: __________
Toxicity Test Concentration(s):
List Any Special Requirements:
Relinquished By: ;
Received
Relinquished
Received By:
Sample Temperature (C°)7
Composite
Time:
Time:_ PM
No ______
------t- __________________________
AM<
Received ByT^^L- -------
Sample Ten^rSure(C°): /\fS
Client Information
CJient: _7gu>h- of _________Pq^
Contact: —r- r-o NPDP0 T-----
Address: P.Q .
City; Phone:
Count^ State: fJcT Zip: —&-Qf---
Type of Facility Generating Effluent:
Sample Information
Sampling Site:
Sample Type:
Sampling Time:
' Date' Tme: PMDate-^^S ^e:PM
Method ofSM^J^WTcTp/uFuPS’
Circle One Other:
Sample Receiving (LaboratoiyU^O^) ’ ----------‘
Received From:
Date:Time:
Sample Condition:
X
START
END
Sample Chilled (Covered with Ice): Yes"
Collector’s Name (Print):h k
Collector’s Signature:
Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 06/28/07IFacility: TOWN OF CLAYTON NPDES#: NC0025453 Pipe#: 001 County:JOHNSTC
Laboratory Performing Test:MERITECH,INC
Reduction:-2.93%CONTROL 2.0% Effluent
# Replicates 12 12
Female Live 12 12
Adult Male 0 0
Adult Dead 0 0
0.00%0.00%I 307 316
Mean # Neonates 25.583 26.333
Standard Deviation 2.811 2.229
Coefficient of Variation 10.987%
Fisher's Exact Test
A = 12 B = 12 b = 12
a/A = 1.00 b/B =1.00
Success is:
8
12 > 8
for the'
I
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA
i Group NeonatesI i Group Neonates Centered
I
Adult Mortality
# Neonates
ORDERED OBSERVATIONS
Centered
for ??2portion1of survival is
--- for the control and the effluent
Test Passes!
C
E
c
c
c
E
E
E
E
E
C
C
22
23
23
23
23
24
25
25
25
25
25
25
-3.5833
-3.3333
-2.5833
-2.5833
-2.5833
-2.3333
-1.3333
-1.3333
-1.3333
-1.3333
-0.5833
-0.5833
13
14
15
16
17
18
19
20
21
22
23
24
C
E
C
C
E
E
E
E
C
c
E
C
-0.5833
-0.3333
0.4167
0.4167
0.6667
1.6667
2.6667
2.6667
3.4167
3.4167
3.6667
5.4167
1
2
3
4
5
6
7
8
9
10
11
12
25
26
26
26
27
28
29
29
29
29
30
31
survival
Critical b value =
The test concludes then,
significantly different
a = 12
of survival is not
------- groups.
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.)
I COEFFICIENTS AND DIFFERENCES
i x(n-i-l)x(i)a(i)x(n-i-1)x(i)
I 1w X 133.2945
141.5833
Calculated W =0.941
Test Passes!I
F test for Homogeneity of Variance
variance 7.9015
= 1.59I4.9697
Numerator degrees of freedom:11
of freedom:11
1.59 s 5.32
of the two groupsare
I
I
PASSES, the variances
same, homogeneous.
=► The Test
significantly the
5.4167
3.6667
3.4167
3.4167
2.6667
2.6667
1.6667
0.6667
0.4167
0.4167
-0.3333
-0.5833
-3.5833
-3.3333
-2.5833
-2.5833
-2.5833
-2.3333
-1.3333
-1.3333
-1.3333
-1.3333
-0.5833
-0.5833
0.4493
0.3098
0.2554
0.2145
0.1807
0.1512
0.1245
0.0997
0.0764
0.0539
0.0321
0.0107
1
2
3
4
5
6
7
8
9
10
11
12
9.0000
7.0000
6.0000
6.0000
5.2500
5.0000
3.0000
2.0000
1.7500
1.7500
0.2500
0.0000
Control F = —------------------
Effluent variance
Denominator degrees
Critical F = 5.32
0.941 S o.884
Critical W = 0.884
EQUAL VARIANCE t TEST
I 25.6 26.3t == -0.724
1.036
Chronic Test PASSES
I
I
I
i
i
There is not c_ M
- and the effluent evaluated
Test passed. T
between the Control
Critical t = 2.508
Degrees of freedom = 22
-0.724 < 2.508
difference in reproduction
------1 at a 99% confidence interval.
Date: 09/21/07
I NPDES#: NC0025453 Pipe#:001 County: JOHNSTON
I #614 & 615 Used
Work Order:
MAIL ORIGINAL TO:
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
% Mortality Avg.Reprod.# Young Produced 23 21 24 18 22 22 26 20 23 24 24 20
Adult (Dive (D) ead L L L L L L L L L L L L
1 2 3 4 5 6 7 8 9 10 11 12
PASS FAIL
# Young Produced 20 23 24 20 25 26 24 26 23 23 24 21 X
Check OneAdult (Dive (D)ead L L L L L L L L L L L L
1st sample 1st sample 2nd sample Complete This For Either Test
Control 7.95 7.93 8.09 7.89 8.03 7.99
Sample 2: 09/13/07Treatment 2 7.97 8.06 8.10 8.27 8.09 8.11
1st
Grab Comp.DurationISample 1 X 23 hrs
Sample 2 X 23.5 hrs
Hardness(mg/1)407.80 7.35 7.76 7.65 8.03 7.63
Spec. Cond.(^mhos)164 1130 11147.87 7.23 7.68 7.77 7.97 7.62
<0.1 <0.1
0.9 1.3
%%%%%%%%%Concentration
%%%%%%%%%%
start/end
Control
Spearman KarberI PH D.O.
Organism Tested:Duration(hrs):
I
Method of Determination
Moving Average Probit
'-- Other High
Cone.
I
Note: Please
Complete This
Section Also
22.25
Control
S
A
M
P
S
A
M
P
0.00
Treatment 2
Control CV
9.981%
0.00
Control
D
I
L
U
T
23.25
Treatment 2
2nd
P/F
e
n
d
e
n
d
e
n
d
LC50 = __________%
95% Confidence Limits
% -- %
Mortality
start/end
Ceriodaphnia dubia
Copied from DWQ form AT-1 (3/87)
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Facility: TOWN OF CLAYTON
Effluent %: 2.0%
TREATMENT 2 ORGANISMS
s
t
a
r
t
1st sample
s
t
a
r
t
1st sample
s
t
a
r
t
2nd sample
Chronic Test Results
Calculated t = -1.146
Tabular t = 2.508
% Reduction = -4.49
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
■ D.O.
£ Control
Treatment 2
Test Start Date: 09/12/07
Collection (Start) Date
Sample 1: 09/10/07 ,
Sample Type/Duration
% control orgs
producing 3rd
brood
100%
Chlorine(mg/1)
Sample temp, at receipt(°C)LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
%
Laboratory Performing Test: MERI'
x ______________—■ ____________
Signature of /Operator in Responsible. Chargee
X __________ __________________
Signature of Laboratory Supervisor
rev. 11/95 (DUBIA ver. 4.41)
PH
I
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
Comments: **Dilution Water Batch
* PASSED: -4.49% Reduction *
MERITECH, INC
4 5 7 9
Sample 1: Sample 2: £O
C_Sample Information
G/C?
U <_cI c
Batch #
L L t 2 5
I*1/1/?~z3 | 2*7 I 2% I Zc>Total Produced Zo
I
11%
pH 1st Sample1 1st Sample2 2nd Sample
2*73ControlL
7.77 0.c(cSample
D.O.1st Sample 2nd SampleLUI/
75>7.«0 ex > 763Control
10/7a?7.2-3 7? 7 7G>2’Sampleu
Temp.
12-4 I 1^ I lb M |t-(p 173 I 1,3 171/ |x[ |Total Produced 70
Control
Comments -<o,■Parti
Sample
1
c
L
c
6
7/(9
C
Sample
2
L
4 5 6 7 8910
|8/'d| /O I u Id I H
Day #2
# Young Produced
Adults Live / Dead
Day #5
# Young Produced
Adults Live / Dead
Day #7
# Young Produced
Adults Live / Dead
L_
3 4
4
O
8
O
u
L-
12
to
12
o
Sample 1
Sample 2
1
Ik
U
L
2
a_c
5
6
0
L_
10
O
C
10
o
L
11
o
c
Transfer Day
Hardness
(mg/L)
Spec. Cond.
(umhos/cm)
Chlorine
(mg/L)
Receipt Sample
Temp (°C)
Test Sample Organism Reproduction
Effluent %:
5
O
8
O
6
O
L| L
5 6 7 8 9 ^10 11 12
9 pA|%i? mi^i^ y
L
C-
V
5
6/7
Zo
^>7^
7^
1
3_
O
g l l
2 3 4
10
/0
L
100% pH
7.79
11
12u
_L.
3
Lk
o
c
3
5 2£zat
2nd Sample
9
O
u
Incubator#:
Date Start: Date End:
Time Start:)1-) ^71^ Time End:
1st Renewal Date: 7 Time:
2nd Renewal Date: 7// 7/6 ? Time:
Analyst(s): Reviewed by:
Collection (Start) Dates:
1st Sample
c
L-
12 34
y/i | ^1^1
7__
O
L
1 2 3 4 5 6 7 8 9 10 11 12
v-?!‘/7?|6 y y/?|
La.
2 8 9
IZ*
U L
11 12
o[ G
G C
L
6 7
iz 111
Duration
~Z-3 hours
23>5^ hours
1st Sample
Z?G 7,^
737
------
1st Sample
ze
Day5
Day 6 ZG
Terminated by: Z^T
?Zi4>7
Meritech, Inc.
Mini Chronic Pass/Fail Test: Ceriodaphnia dubia
------------ Pipe#:_Ccl_ County:
Date / Time of Culture Transfer: e/ - / y
Date / Time Neonates bom: ' ^r-/
-? Age of Neonates at Test Start: 73, hours
________ I Culture Tray Temp: ‘2 s' °C
L C
11 12
L
Transferred by: Fed by:
Day 0 M LL,
Day 1
Day 2 l/ll^
Day 3
a - ^.q/p^DayA
<C/jkd.|
c.7 V3
Client: T
NPDES #: NC co z Tc/
Dilution Water: Lake Reidsville
Test Organism Source: Tray #______
Stirred / Aerated for D.O.: Y /(^) Randomized:^/ N
Control Organism Reproduction
Day #2 1 2 3
# Young Produced
Adults Live / Dead
_______Day #5
# Young Produced
Adults Live / Dead
_______Day #7
# Young Produced
Adults Live / Dead
OSII^0I
I
Zip:
Type of Facility Generating Effluent:
i
I
Toxicity Test Information
i
I

Shipping Information
/
I
I
Relinquished By:
Received By:
Relinquished
Received By:
Sample Temperature (C°):
Sample Information
Sampling Site:
Sample Type:
Sampling Time:
'PM
PM
Received By: / —-----
Sample TemperaW^C0)^; ,5 47g
Test Required: Chronic:
Acute:
Client Information
Client: 7~o^) h-
Contact:
Address:
City:
County:
1 Phone: /
Pipe Num
%
Tox/c. (
/ /1 np, J.
PO#__________ ____
j NPDES #: /t/fS <7 5~'tA5~~3

er: f
START
END
Sample Chilled (Covered with Ice)'. Yes
Collector’s Name {Print):
Collector’s Signature:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]

# of Containers
PM
/O-'OO PM
---------------------------1—I
Composite
Time:
Time:
No_
y/on I A

Date: ^////o y
Date: 9^2)
Date:
Date:
Test Organism: Ceijiodaphnia Dubia:
Pirriephales promelas:
Myisidopsis bahia:
Circle One
Sample Receiving (Laboratory Use Only)
Received From:
•h
Time: //.yfc)
Time: AM PM
j ——------ Time:______AM PM
Method of Shipment: WTC(p/u) UPS Fed-Ex
Other:
4--------------~ —
| ---------------
I
I
Date: Time: cw
Sample Condition: " f Cq!)
WS C :
Toxicity Test Concentration(s):
List Any Special Requirements:
__Cl^' pL_<CL-a? f)
yp le^^ e, .
'7~Oujh. c>
_-4 e- s>
'P-O, 6ox ^7^
^Tonn S J''* *7
_______ Grab____
Date: 7
Date: ?-//- 0 7
GLAr f-e. +~\
/D Hrk* *1
State: /(/
I
Zip:
; Type of Facility Generating Effluent:

Shipping Information
Test Required:
Sample Information
Sampling Site:
! Sample Type:
! Sampling Time:
Chronic: X
Acute:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]

Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
I
—--------------------- -
# of Containers
3(3
ure (C°): /. 3 /
/3/
- Sa.
X Composite
Timej: _
0/
Grab _
Date:
Date: ‘?-/‘/-Joo'7 Timd:
___No____
/fo^ae^. (x ,
Relinquished By:
Received By:
Relinquished
Received By:
Sample Temperature (C°):
I
I
____
h^M)PM
~r<?
'^y Time:
Time:^AM PM
it: WTC(p/u) UPS Fed-Ex
i Other:
IWC:
1 Toxicity Test Concentration(s):
List Any Special Requirements:
AM

Sample Receiving (Laboratory Use Only)
Received From:
Client Information
, Client: 7^0 h
Contact:
Address: To Bqk
City:____S-(
; County: ^-o
Time: _^^57AM/fS?
Time: /<?73'AM PM
! 'cXy Time: ^T^AMCPmE
2^ Date: 7
Date: / t
Date: ^7^-/
Date: T 1 -
Method of Shipmeni
Circle One
e f __________ pQ^
■Tq>—»<^ S Lj<x^-re,M I<JPDES #:
—J- f)S-f- Phone:
-------------------------------------- Pipe Number:
----- State: . Zip:
I
START
END
j Sample Chilled {Covered with Ice): Yes
Collector’s Name {Print):
Collector’s Signature: ~
- ' ' Z——
Toxicity Test Information
Received By:
I Sample Tem^Date:
Sample Conditiop:
Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 09/21/07
Facility: TOWN OF CLAYTON NPDES#: NC0025453 Pipe#: 001 County:JOHNSTON
Laboratory Performing Test:MERITECH,INC
Reduction:-4.49%CONTROL 2.0% Effluent
# Replicates 12 12
Female Live 12 12
Adult Male 0 0
Adult Dead 0 0
Adult Mortality 0.00%0.00%
# Neonates 267 279
Mean # Neonates 22.250 23.250
Standard. Deviation 2.221 2.050
Coefficient of Variation 9.981%
Fisher's Exact Test
B = 12 b = 12
a/A = 1.00 b/B = 1.00
Success is:survival
Critical b value =8I12 > 8
Test Passes 1I
SHAPIRO-WILK'S TEST
I
ORDERED OBSERVATIONS
i Group Neonates Centered i Group Neonates Centered
I
c
E
E
C
C
E
C
C
C
E
E
E
18
20
20
20
20
21
21
22
22
23
23
23
-4.2500
-3.2500
-3.2500
-2.2500
-2.2500
-2.2500
-1.2500
-0.2500
-0.2500
-0.2500
-0.2500
-0.2500
C
C
E
E
E
C c c
E
E
E
C
23
23
24
24
24
24
24
24
25
26
26
26
0.7500
0.7500
0.7500
0.7500
0.7500
1.7500
1.7500
1.7500
1.7500
2.7500
2.7500
3.7500
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
not
-c groups.
The test concludes that
significantly different
the proportion of survival is no
for the control and the effluent
a = 12A = 12
FOR NORMAL DISTRIBUTION OF DATA
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.)
COEFFICIENTS AND DIFFERENCES
i x(n-i-l)x(i)a (i)x(n-i-1)x(i)
1
W X 96.3833
100.5000
Calculated W = 0.959 Critical W = 0.884
0.959 a 0.884
The reproduction data is normally distributed
evaluated at a 99% confidence interval.
Test Passes!
F test for Homogeneity of Variance
Control variance 4.9318F == 1.17=
Effluent variance 4.2045
Numerator degrees of freedom:11 Denominator degrees of freedom:11
Critical F =5.32
I 1.17 s 5.32 =► The Test PASSES,
are
I
the variances of the
significantly the same, homogeneous.
3.7500
2.7500
2.7500
1.7500
1.7500
1.7500
1.7500
0.7500
0.7500
0.7500
0.7500
0.7500
-4.2500
-3.2500
-3.2500
-2.2500
-2.2500
-2.2500
-1.2500
-0.2500
-0.2500
-0.2500
-0.2500
-0.2500
0.4493
0.3098
0.2554
0.2145
0.1807
0.1512
0.1245
0.0997
0.0764
0.0539
0.0321
0.0107
8.0000
6.0000
6.0000
4.0000
4.0000
4.0000
3.0000
1.0000
1.0000
1.0000
1.0000
1.0000
1
2
3
4
5
6
7
8
9
10
11
12
two groups
I
EQUAL VARIANCE t TEST
22.3 23.3
t = = -1.146
0.873
Degrees of freedom = 22
Critical t = 2.508
-1.146 < 2.508
I
Chronic Test PASSES
I
There is not a < ' ''’
-- effluent evaluated at
significant difference in reproduction
a 99% confidence interval.
Test passed. 1D
between the Control and the
‘SERVICE"
’’ENV1RONMEN1
July 23, 2007
Re: Fat Head Minnow Toxicity Results
To Whom It May Concern:
Enclosed please find the fat head minnow toxicity results for the Little Creek Water
Reclamation Facility. The additional sampling is being conducted due to the NPDES
permit renewal.
Sincerely,
I James Warren, ORC
lyi I' 30 - f ->;_i '<•
VEHICLE MAINTENANCE
[phone]
ELECTRIC SERVICE
[phone]
TOWN OF CLAYTON
OPERATIONS CENTER
DWQ DEHNR
Aquatic Toxicity
1617 Mail Service Center
Raleigh, NC 27699-1617
water reclamation
(919' S53-!5.',5
PUBLIC WORKS
[phone]
Please call me directly at 919-553-1536 if you have any questions.
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date7/6/2007
Facility: Town of Clayton WWTP NPDES. NC0025453 County: Johnston
x
Signature of Operator in
I
MAIL ORIGINAL TO:
I
Test Initiation Date/Time 6/26/2007 /3:30 PM Avg Wt/Surv. Control [0.4771
% Survival [100.0|
0.477|Hatch Date:6/25/07
[lOO.Oj Hatch Time 3 00 pm CT
Avg Wt (mg) [
[% Survival [
Avg Wt (mg)[ 0 4491
[% Survival [100.0|
Avg Wt (mg)[0.529|
100.0|% Survival f
Avg Wt (mg)[0.483|
[% Survival [100.0|
Avg Wt (mg)[0.577|
I

I

DWQ Form AT-5 (1/04)
1
6/25/2007
8.17
7,63
24.9
2
6/26/2007
6.02
7.15
Avg Wt (mg)[
% Survival [
Dilution H2O batch
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Cultured In-House
Outside Supplier
8J Surviving #
Original #
Wt/original (mg)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) IniVFin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp, at Receipt (°C)
41 Surviving #
Original #
Wt/original (mg)
2| Surviving #
Original #
Wt/original (mg)
11 Surviving #
Original #
Wt/original (mg)
0.5| Surviving #
Original #
Wt/original (mg)
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Growth
Fl
Fl
Normal
Hom Var.
NOEC
LOEC
ChV
Method
Environmental Sciences Branch
Division of Water Quality
NCDENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Survival
rin
Test Organisms
r
24.00
62.00
92.00
589
<0.1
1.3
157
42.00
61.00
207
Pipe #: 001
Comments |
0.512|
100.0|
7.88
7.39
25.0
x
Signature of Laboratory Supervisor
8.09
7,68
24.9
10
10
0.644
10
10
0.583
10
10
0.472
10
10
0.491
10
10
0.484
1
10
10
0.452
10
10
0.586
10
10
0.538
10
10
0.478
10
10
0.453
10
10
0.387
10
10
0.560
10
10
0.582
10
10
0.442
10
10
0.645
10
10
0 460
7 88
710
25.0
10
10
0518
10
10
0.479
10
10
0.393
10
10
0.474
10
10
0.531
Stats
Cone.
0.5
1
2
4
8
Survival
Critical
10
10
10
10
10
8.00
7 74
24.9
8.11
770
24.9
Calculated
18
18
18
18
18
8.19
7,96
25.1
8.20
7.76
24.8
Critical
241
2.41
2.41
2.41
2.41
2
10
10
0.558
3
6/28/2007
23.50
66,00
102 00
650
<0 1
2.0
3
10
10
0.423
4
10
10
0.474
7.90
7 17
25.0
23.00
62.00
96,00
592
<0.1
1.1
8
>8
>8
Steel's
8
>8
>8
Dunnett's
0
/ 7.98
/ 7.00
I 25.0
______2
8.17 /
7.60 /
24 9 /
Laboratory: Meritech, Incy—
ispoos'ible Charge
______1_
8.20 /
7.56 /
25.3 / 25.0
158
40
56
196
% Eff. Repl.
| Control | Surviving #
Original #
Wt/original (mg)
______4
8.13 /
7.56 /
25.0 /
2______
/ 7.96
/ 7.15
/ 25.0
5
/ 7 92
/ 7,46
/ 25.0
Growth
Calculated
•0.7712
0.6060
•1.1569
-0.1322
-2.2091
1
8.11 / 7.87
7.52 / 7.06
25.3 / 25.0
______4
8,10 /
7.58 /
25.0 /
6______
/ 8.02
/ 7.45
/ 25.1
5
/ 7,90
/ 7.56
I 25.0
6______
/ 7.99
/ 7.58
/ 25.0
3
8 18 / 7 92
7.65 / 7.18
24.8 / 25 1
Overall Result
ChV | >8
0
8.09 / 7.95
773 / 6.87
24.9 / 25.0
Day
3
8 15 / 7 92
7.58 / 710
24.8 / 25.1
Client: Time: 3# of Organisms per Chamber: 10 Start: Date:
NPDES#: NC 002-5'^5'3 Test Vessel Size: 400 ml End: Date: Time:
Test Solution Volume: 250 ml
Temp, of Stock: Date/Time Fed:Randomization:Ye:No
3 -4^' ^ IDate/Time Born:# of Reps: 4 Incubator #:
Transferred by:Fed by:
Time Initials Date Time 1 Initials Time 2 Initials Time 3 Initial
12:^faDay 1 Day 0
Day 2 Day 1 ’,20^
4/2.Day 3 /H^rti^Day 2 mi-
Day 4 /ylODay 3 >A-^
Day 5 /MLDay 4 4:^*
^l/2-Day 6 7'L^Day 5 /|.w<
Z^lz ^'Sb=^Day 6
Test Termination Data:
Initials/Signature:
End Date:
End Time:
l O'. 30
°C
Mentecn, !nc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Date
^4?
'VzS'
4/z£>
•k
= Weekend, only 2 feedings needed
Initiated by: Z^, //I/1"
Jo?
w
I
I
Page:of 2
Outfall:
Date of Test:I
by: /ttc Organism: Pimephales promelas
by: ML
CONG REP # ORG.SURV %
C
'799,7/804.23A O,M£T2 loo
800.03 eofc.aiB 5.58 /O O,53~p,ICQ
803.29 4.23c lo IOQ
79g, 3'4.74D803,05 o.H?Hio IO0
■1^.2^804.23 4.84A zo (.1)0
B 8c4.<oC 3.fe7I /o .0.387 (DO
c 2.45-.50 /0 IDO
777 77 603 08D 5.2>i 10 0.53 ((0OI8oo.4lA 805.32 4.7 I SL 0471 /DO
\.O7.E-o 4.07 8C6.G2.B 4.S3 10 100
c 4I!Q /0 /DO
orqqD 3.93 (0 /DO
PAN WT.
(mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF ORG.
(mg)
O
A/
PAN + ORG.
WT. (mg)
MEAN
WT./ORG. (mg:
/We.
ENVIRONMENTAL LABORATORIES
D>vu(on 0/ UVater Technology /tnc! Controls, inc
0
L
Facility: CLVi\ rVe>W_______
NPDES #: AJG 00^-0 953
^4-^ /p7
Initial weights taken on: 6 /07
Final weights taken on: 7 /5/o7
I
Facility: Page: Z- of 2
Outfall: op /
Date of Test:
Organism: Pimephales promelas
CONG # ORG.SURV %
fell.toO ^■83 Zo loo
2.0 7,ec4.oiTO 23 418B IO /op
&03Si Ic 5./o 0.^82-too
^98,42D ec3.IL 4.74 IO e>,H7‘/loo
gq Boz.fciA 4.7^/o 6.472 100
^O Z SC^.B 5.2I o 6.too
8/4.17c 442,/o Q-442-tooIBoa, s'iD 4/1 io tooI601,33 807.-74A io 0-0^loo
8,0 '/>801.34B 80^.20 5.8G io too
Bcsnsc 8H.35 5.00 10 0.580
^.43 8o4.WD 5.18 o.s/Bo to
I
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF ORG.
(mg)
PAN + ORG.
WT. (mg)
MEAN
WT./ORG. (mg)
by: ACC
by: ACC
PAN WT.
(mg)
805. nr]
sv/eritec:^,
ENVIRONMENTAL LABORATORIES
M Div.zion Of l^.ter Technology and Controls. Inc
REP
LU-'
A
w Qrv________
NPDES #: eOZ.5^53
/>_&> /t>p_______
Initial weights taken on: G/29/07
Final weights taken on: 7/6/07
I /a >
Analyst(s):
2 3 5 6 7 Remarks
I PH
D.O.
Temp.
Conductivity
Remarks
D.O.
Temp.I
Conductivity
3 7 Remarks
D.O.
Temp.
Conductivity
Concentration:
PH
___Da;
4
B.2c
Time: 3!3o^
Time:
2^.0
i
Concentration: /. O
pH Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
Concentration: control
__ Batch #
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
_______Hardness
Alkalinity
6.^7,
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
1
8.o4
231
^2.
-11L
n.b3
7,/3
ZS.O
200
2/ (p
^>1
6
6.03
~7.-76>
^9.7
ZS'.O
230
I
~^o./
5
a H
"7^ 3
25.0
2^(2
21
3^3
i
/n 7
<7-0^
7.00'
2^.0
2-1 (o
22g
zo, I
—fel
7
e./u n/n
7.93
7,
25.1
I
8 2-0
7/^
2S.3
2C5
22^
<G.|
2
6.29
7.75
7.00
7.U
2^.3
1^.0
2o3
<CM
3
22-
7.75
(p.38
25.0zn|
Day
4
j£8_
6.15
7.Q2.
7.55
7. ic
25 a
I
z-O. I
£k
g.//
'1/12-
Z~7O
■7.4m
Z4.7
/g-?
7.66
7.39
aso
117
17^
5
8.20
?.&7
7.03
7.I&
25.0
2£0_
M
27^
7,57
7> 05“
2M.e>
151
3c^r
22b
60.1
2
8.2.1
W
7.57
7.05
8.20
8.02>
7.50
7.75
2-7.^
25.1
^0/
1
<9,1^
6.0S
7,82-
7. io
2.9.0
zz.^
33 Z
^0.1
Day
4
e, is
■7.^8
^5
^.e>
|£l^
.72 •2,
z-O. I
6
6i<
n/ig
7.72-
7.52
zy.f
Zv.O
2^
^a/
IVlEFtlTECHr JNC.
ENVIRONMENTAL LABORATORIES
A Di^sion Gf V^t^r r&chr.oloay and Control.
Chemical and Physical Determinations
Client:__^^\x>opf7p r}--------------- NPDES#: MC qq z^^T^Start Date: ____
Test Organism: famephales promelas Analystfs): mr End Date:
9.94
W,
9.39
79,fi
25,1
20.1
I
Chemical and Physical Determinations
Remarks
I D.O.
Temp.
Conductivity
I Remarks
D.O.
Temp.
Conductivity
Remarks
D.O.
I Temp.
Conductivity
2 6 7 RemarksI
MERffTECH, iraC.
envinorurx/iEr\j-rxKL laboratories
Page 2 of 2
NPDES#: A/C DO^^5^3 Test Dates: Start: 644/07 EndLStaO/)*-'
Analyst(s): di£ ^il Test Times: Start: End: /ofa^^-
Concentration: gto z
pH___Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
Concentration: Z
pH__________Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
Concentration: 100%
pH
__________Conductivity
_____Residual Chlorine
__________ Hardness
Alkalinity
Concentration: /.
pH__________Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
3
7.53
27-7
2530
22-V
Zc?. I
1
7.3 7
^72-
, 1
L.2-
2
6.16
7.71
754
7.^9
2S~.3
2io
^-O.l
3
79/
7.56
^20
I
7-13
7,^9
zo. 1
Day
4___3
79?
20,/
-SUz.
____7
8J5
7.AZ
7_CJ_
2^.g>
26.\
231
ZO.|
5
7^0
<0.1
(nt#
____7
p. 19
799
79^
25.\
a
zqq
2^
A
1
6.09
7.95
7, 73
L.09
2-9.9
2S<0
2 36
25-5
Zo, I
1
84 q
7.9fe
7,73
6/iz
2M/|
230
25 3>
z.0./
2
8. ik
7 89
7 52.
3>
Z$~.O
2^0
Z37
^o. I
■
^.10
JL.52
7.6?
7.5
29.7
ZS3O
<739
z-93
^o.)
3
a/1
7.68
7 L-O
-1-.1C
297
292-
257
Z'e>./
Day
4
& IB
7.87
760
7/5
5
R l(*
7.87
766
73Q
22^
MO,
^6 |
6
-BLOO
7.90
7.77
758
ZSTQ
MfL
I
8. (5
8.O{
7.P2-
-Z-9.9
■2-5.0
22 A
Z O. |
Day
4
g. io
789
7.62.
5J./.
-2-y.p)
25-1
73T~
Zt),/
5
8. Io
7.90
7.58
5? J 9
25.0
____7
<4
796
7. 87
7 56
29,8
25. \
22-2^
40 . I
Day
4
6J8
79Z
765
^.8
-25-i
23V
7AT~
zo.)
Client: OAcTvjVbfl__________
Test Organism: Pimephales promelas
6
y./t,
_2M_
7. ?.$'
■1.55
29.7
Z.5-.0
X?>C>
2.^
£<>■/
5
8../O
zb 7
7.60
7.2 Z
25.0
'2S.O
2
8. K
787
7, 52
zLa?
23.3
2^.0
273
AO, I
I Client:
/07End Date:End Time:
Concentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
o Rep #1 G OQo o o
OO0Rep #2 O Gn o
0 ORep #3 0 OoIC)o
o 0Rep #4 o oo O
Concentration I
Day 7Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6
Rep #1 O o oooc.)o
0Rep #2 oo oo
0Rep #3 O oo i O
o DRep #4 Oooo
Concentration
I Day 0 Day 2Day 1 Day 3 Day 4 Day 5 Day 7Day 6
oRep #1 Oo o 0o o
Rep #2 0 0 OO oo
I Rep #3 f} Q O 6?Q O a ! O
Rep #4 0 0 0oooo 0
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client:lA
End Date:End Time: b 7
2.0Concentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
Rep #1 C>O O
o oo o
o oRep #2 O a oo c>o
ORep #3 O 0I oo oe>
oRep #4 O ooo o o 0
^.0 /Concentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6
Rep #1 C>C Q oc>o o o
oRep #2 o Oc>Oo o
oRep #3 O o o o o o
0Rep #4 OoO c>o ; o
8.0 7.Concentration
Day 0 Day 2Day 1 Day 3 Day 4 Day 5 . Day 7Day 6
Rep #1 Co O 0 o o o 6
O ORep #2 0 O O OO O
c>0Rep #3 o oo o i o
0 0 o0Rep #4 O o Co
!:
: Day 7
IVICKII tun, INU.
Mortality Data: Chronic Fathead Test
Start Date: Start Time: 3:3Op^.
062^I
State:
Type of Facility Generating Effluent: W id'T C ! <i 3 s J F
I
Test Required:
I

Shipping Information
I
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:
Chronic:
Acute:
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:

Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
Relinquished By:
Received By:
Relinquished By:
Received By:
Sample Temperature (C°):
Received By: ( .CxA,.
Sample Temperature (C°): /.//A n

Toxicity Test Information
AWM
AM PM
Fed-Ex
<=> -F
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements:
Date: Ip ]2^/0} Time:^^/O
Sample Condition: /
- Circle One
Sample Receiving (Laboratory Use Only)
Received From: Z2
Client Information
Client: Ttfutn e> f (J^l^
Contact:
Address: PzP.goy. f?? 7)
City;___
CoLinty: y ^0
_____PO#
NPDES #: 3"tS5' 7
Phone:
Pipe Number: o (p J
Zip: SSL 8
^TDate: Time: ll.^o
- Date: Time: //.yp
Date: Time:
Date: _______ Time:
Method of Shipment: WTC(p/u) UPS
Circle One Other:
# of Containers
/’ff : 00 ^PM
& AM PM
F f [ vc<S _ ___________________

------------Crab \ Composite
START Date: 7 Time: _
o . END Date: /p y Time: _
Sample Chilled [Covered with Ice): Yes No
Collector’s Name (Print):e
Collector’s Signature:
oo !
Zip:
Type of Facility Generating Effluent: uJ u? 7~ P
Test Required:X
I

Shipping Information
I
I
.
Received By
Sample Term
Relinquished By:
Received By: &
Relinquished By: t
Received By:
Sample Temperature (C°):
Sample Information
Sampling Site:
Sample Type:
Sampling Time:
Chronic:
Acute:
START
END
PM
PM
Meritech, Inc.
Bio as say Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]

PO#
NPDES #:
Phone:
Pipe Number:
^7^ 8^
AM PM
Fed-Ex
3
_<^PPM
.AM PM
# of Containers
C> 700
0^ OQ

Toxicity Test Information
Cl
Cj, !^r~_____
X Composite
Time:
7 Time:
X No
IWC: ______________
Toxicity Test Concentration(s):
List Any Special Requirements:
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas: X.
Mysidopsis bahia:
Received From:
Client Information
Client: To h
Con tact: X '
Address:fQOQ D
City:____I __________________
C ounty: State:
Sample Receiving (Laboratory Use Only)
2 Date: y/o’] Time: /^/#5
- Date:/^7 /py Time: /£?/
Date: 7 Time: ZTZ# AM@>
Date:Time:
Method of Shipment: WTC(p/u) UPS
Circle One Other:
Grab _
Date:
Date: _
Sample Chilled (Covered with Ice): Yes
Collector’s Name (Printy. /.-Il di
Collector’s Signature: ~/. C
06>3i
State: Zip: I Type of Facility Generating Effluent: $ JjZ.
E-P-fl

Toxicity Test Information
Test Required:
I
I
I
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:Time:
Time:
No
Client Information
Client: _
Contact:
Address:
City:
County: _
Chronic: %
Acute:
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas: X
Mysidopsis bahia:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
AM PM
Fed-Ex
^.0.3
■felt * 3 ^~e
4. Q»^f
Date: (J'
Samp 1 e (condition:
Grab X Composite
Date: 7
Date:
X
e. a ___
JTime: 3'^ AM^Z?
IWC:________________
Toxicity Test Concentration(s):
List Any Special Requirements:

<5 f (fttB'y
OX S' 2?/xoo f)
START
END
Sample Chilled {Covered with IceY Yes
Collector’s Name {Print): A
Collector’s Signature: .
Shipping Information

Relinquished Time
Received By: Date: Time:
Sample TempeTalUT^ZZ^n^thoVofSi^^WrcZuTuPS
- Circle One Other:
Sample Receiving (Laboratory Use Only)
Received From:
Received ByT'"^^^ (^7
Sample Temp^ture (C°): guD /^Z)
'D.D/cl.O

PO#
NPDES #: 00 x y/5~3.
Phone:
Pipe Number: gg? /
# of Containers
^^30 cAM)PM
0 AM PM
I Transform:NO TRANSFORMATK
Shapiro - Wilk's Test for Normality
I
Data PASS normality test (alpha = 0.01). Continue analysis.
I
I
I
I
D =
W =
Critical W
w
o. 0741
0.9778
= 0.8840 (alpha = 0.01 ,
= 0.9160 (alpha = 0.05 ,
N = 24)
N = 24)
Title:
File:
clayton
clayton
State: Zip:
uJ 7"Type of Facility Generating Effluent:1^55
Test Required:
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:
Chronic:
Acute:
START
END
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
Client Information
Client:
Contact:
Address:
City:__
County:
Time^V, y* PM
Time:
Time:
Time:
PO#___
NPDES #:
Phone:
Pipe Number:
3
PM
pm
# of Containers
//.• oo
Toxicity Test Information
Time: "VSO AM
31^ JI
A
Meritech, Inc.
Bioahay Sample Chain of Custody
642 Tamco Rd
! Reidsville, NC 27320
[phone]
Composite
Time:
Time:
No
/z CLz 3 gr
Shipping Information
Relinquished By: i t/,
Received By:
Relinquished By:
Received By:__________
Sample Temperature (C°):
u-e ____________
Grab X <, ~
Date: o 7
Date:
Sample Chilled (Covered with Ije): Yes
Collector’s Name (Print)-.
Collector’s Signature: _
Date:
Date: 7
Date: > y^o ?
Date: Time:AM PM
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
Sample Receiving (Laboratory Use Only)
Received From:
Received f iA- Date.
Sample Tempertrttfre (C»): h^/l1^ Sample Condition:
C
A~b Pt
City:
I
Toxicity Test Information
I
Date:
Type of Facility Generating Effluent:
--------------- -------- ■ i =
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:
Date:
Samp 1 e Condition:
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
START
END
Sample Chilled (Covered with Icz)'. Yes
Collector’s Name (Print)'.
Collector’s Signature:
Received By: (
Sample Temperature (C°): '
i
Shipping Information
Relinquished By: Z^^^Date:
Recelved / Date:
Re 1 i n q u i s
Received By:^ C?
Sample Temperature (C°):
Sample Receiving (Laboratory Use Only)
Received From: /?///
Etf’-Ci'LA.d. ________________
Gi;ab a-—' Composite
pate: 7/27
Date: 7
Time:
Time: AM(gM
t - Time^/y-) AM PM
- Date: _________ Time:AM PM
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
__ PO#
NPDES #:
£4- Phone: (w) -/T'J? dr­
pipe Number: ocd /
Zip: fT
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
, Reidsville, NC 27320
[phone]
IWC: ________________
Toxicity Test Concentration(s): J
List Any Special RequirementsT

I
Time:c
Client Information
Client: /jtC) ia. ty-f' CyidLy
Contact: _______
Address: "P.O,
C^y:___(L( ______a__
County: 4o)*Zs Zqh State: /xf*
Time:
Time: 0
No_____
# of Containers ^3
<£> ?OQ PM
PM
Test Required: Chronic:
Acute:
I
Toxicity Test Information
X
Shipping Information
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
# of Containers
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements:
Received By:
Sample Tempe
___
^L
AM PM
AM PM
START
END
Sample Chilled {Covered with |Zce): Yes
Collector’s Name {Print):
Collector’s Signature:
3rab
Date: '7/2G/01
Date:
~'es
Relinquished By: Xr
Received Bj
Relinquishefray:Z3*-*
Received By:
Sample Temperature (C°):
Time: I AIv/pm)
Date: Time: Z2-72- AMC®
_ Date: Time: (
Date: 7/3-7/a 7 Time: //J± AM@)
_ Date: Time:AM PM
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
Sample Receiving (Laboratory Use Only)
Received From:
po#
NPDES #: aJC 00 2.5/1/,
Phone:
Pipe Number: f) (
'r3~<a
Client Information
Client: o -P" f - - T_____________
Contact: & s____LJ cvr-tHc- ______________
Address: (D . y ft?*] 7/0 0 0 SZ
City: C (7^^/_________ _________ ___________
County: ' I State: A)^ Zip:
I
Type of Facility Generating Effluent:
1
Composite
Time:
Time:
^o^
uXXj _______
Date: 7^1/
Samp 1 e Condition:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
I _______________________________________
_ j. -
Test Required: Chronic:
Acute:
Transform:NO TRANSFORMATION
Shapiro - Wilk's Test for Normality-
Data PASS normality test (alpha = 0;. 01). Continue analysis.
I
i
i
I
I
I
I
I
N = 24)
N = 24)
Title:
File:
D =
W =
0.0209
0.9842
I
I
i
i
I
I
Critical W
W
= 0.8840 (alpha = 0.01 ,
= 0.9160 (alpha = 0.05 ,
-------L—
clayton
clayton
NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated Bl statistic =(p-value = 0.7116)2.9244
)1 level. Continue analysis.Data PASS Bl homogeneity test at 0 .
I
I
II
I
Title:
File:
(alpha = 0.01
(alpha = 0.05
5)
5)
Critical B = 15.0863
= 11.0705
I
I
Transform:
df =
df =
clayton
clayton
NO TRANSFORMATION
FMSSSSOURCEDF
I 0.0025 2.16040.01265Between
Within (Error)0.0209 0.001218
Total 0.033523
(p-value = 0.1045)
Critical F
Since F < Critical F Ho: All equal (alpha = 0.05)FAIL TO REJECT
I
I
L
I
I
ANOVAj Table
----1-----
i
i
[Transform:
Title:
File:
= 4.2479
= 2.7729
(alpha = 0
(alpha = 0
df = 5,18)
df = 5,18)
i
i
!
.01,
. 05,
clayton
clayton
''
NO TRANSFORMATION
Dunnett's Test
Ho:Control<Treatment
GROUP IDENTIFICATION
T STAT
Dunnett critical value = 2.4100 (1 Tailed, alpha = 0.05,df = 5,18)
NO TRANSFORMATION
TABLE 2 DF 2 Ho:Control<Treatment
GROUP IDENTIFICATION
I
II
Title:
Fixe:
MIN SIG DIFF
(IN ORIG. UNITS)
Title:
File:
1
2
3
4
5
6
1
2
3
4
5
6
NUM OF
REPS
4
4
4
4
4
4
TRANSFORMED
MEAN
0.0581
0.0581
0.0581
0.0581
0.0581
MEAN CALCULATED IN
ORIGINAL UNITS
0.4775
0.4480
0.4925
0.5233
0.4990
0.4955
% OF
CONTROL
12.2
12.2
12.2
12.2
12.2
DIFFERENCE
FROM CONTROL
1.2234
-0.6221
-1.8973
-0.8916
-0.7465
0.0295
-0.0150
-0.0457
-0.0215
-0.0180
SIG
0.05
control
. 5
1
2
4
8
I
II
I
control
. 5
1
2
4
8
0.4775
0.4480
0.4p25
0.5?33
0.4990
0.4955
iTransform:
TABLE 1 OF 2
! Transform:
clayton
clayton
clayton
clayton
Dunnett's Test
Transform:NO TRANSFORMATICI
Steel's Many-One Rank Test Ho: ControlcTreatment
GROUP IDENTIFICATION DF
I
Critical values are 1 tailed !( k 5 )i
i
I I
I
i
I
clayton
claytonsurv.
Title:
File:
i
l
RANK
SUM
18.00
18.00
16.00
18.00
12.00
CRIT.
VALUE
10.00
10.00
10.00
10.00
10.00
SIG
0.05
1
2
3
4
5
6
I
I
10.0000
10.0000
10.0000
9.7500
10.0000
9.0000
4.00
4.00
4.00
4.00
4.00
I
i
control
0.5
1
2
4
8
MEAN IN
oklGINAL UNITS
I “SERVICE”
“ENVIRONMENT"
I
8/27/2007
I
Re: Toxicity Results
To Whom It May Concern:
I
Please call me directly at 919-553-1536 if you have any questions.
James Warren, ORC
Enclosed please find the Chronic Fathead minnow toxicity results for the Little Creek
Water Reclamation Facility. The additional testing is due to the NPDES permit renewal
process.
TOWN OF CLAYTON
OPERATIONS CENTER
VEHICLE MAINTENANCE
[phone]
ELECTRIC SERVICE
[phone]
WATER RECLAMATION
[phone]
PUBLIC WORKS
[phone]
DWQ DEHNR
Aquatic Toxicity
1617 Mail Service Center
Raleigh, NC 27699-1617
Sincerely,
653 Highway 42 Wes. • P.O. Bex 879 • E^y.on. N.mh Carolina 27520 • <919,553-.530 • Fax .9i9) 553-TT
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date:8/21/2007
Facility Town of Clayton NPDES # NC0025453 Pipe # 001 County' Johnston
•atoiy Meritech, Inc Comments
MAIL ORIGINAL TO:
Test Initiation Date/Time 8/7/2007 /4 00 PM Avg Wt/Surv. Control [0.439|Test Organisms
100.0|% Survival [
Avg Wt (mg)[0 439|Hatch Date 8/6/07
% Survival F 100 0|Hatch Time: 3 pm CT
Avg Wt (mg)[
I % Survival [
Avg Wt (mg)[
% Survival [
Avg Wt (mg)[ 0 4781
% Survival [100.0|
0.4511Avg Wt (mg)[
% Survival [100 o|
Avg Wt (mgjf 0 492|
I
I DWQ Form A 7-5 (1/04)
1
8/6/2007
10
10
0 402
10
10
0 480
10
10
0.637
10
10
0 459
8.08
7 45
25 0
8.07
7 62
25.0
8.21
7.47
25.2
10
10
0 473
4| Surviving #
Original #
Wt/ongmal (mg)
Dilution H2O Batch
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
2| Surviving #
Original #
Wt/original (mg)
0 5| Surviving #
Original #
Wt/onginal (mg)
11 Surviving #
Original #
Wt/ongmal (mg)
Cultured In-House
Outside Supplier
^8J Surviving #
Original #
Wt/original (mg)
Water Quality Data
Control
pH (SU) IniVFin
DO (mg/L) Init/Fin
Temp (C) IniVFin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp, at Receipt (°C)
High Concentration
pH (SU) IniVFin
DO (mg/L) IniVFin
Temp (C) IniVFin
Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Normal
Hom Var
NOEC
LOEC
ChV
Method
8.16
7.54
25.1
10
10
0.399
0 4481
100.0|
0.515|
97.51
X -------
^gn^ture of Operator in Responsible Charge
x
Signature of Laboratory Supervisor
# 167
42 00
60.00
227
10
10
0.435
10
10
0 426
10
10
0.622
9
10
0 501
10
10
0 460
1
10
10
0.535
168
42
62
209
2
8/7/2007
10
10
0 468
10
10
0 423
10
10
0 354
2
10
10
0 453
8.07
7 42
24 5
169
40
62
214
10
10
0.603
10
10
0.497
3
10
10
0 393
170
42
60
204
10
10
0.463
10
10
0.459
10
10
0 470
10
10
0.503
4
10
10
0.375
Survival
Critical
10
10
10
10
10
8 06
7.54
25.0
Calculated
18
18
16
18
18
22.50
58.00
146,00
896
<0 1
0.9
23.00
58.00
140.00
883
<0 1
1.2
______2
8.17 /
7.52 /
25.2 /
3
8/9/2007
8 00
713
25.0
8 05
775
25.0
8.09
7.75
24 9
8.20
7 70
24 9
% Eff, Repl.
[Control] Surviving #
Original #
WVongmal (mg)
24,25
58.00
131,00
872
<0 1
0.5 Stats
Cone.
0.5
1
2
4
8
8.09
7 71
25.0
1
8 18 /
7 44 /
25.2 /
3
/ 8 07
/ 7 41
I 25.0
4
/ 799
/ 7.55
/ 25.0
______4
8 15 /
7 65 /
25 0 /
2______
/ 7 99
/ 7.34
/ 25 0
6______
/ 7.92
' 7 52
/ 24 9
6______
/ 7.98
/ 7.59
/ 24.9
5______
/ 7.99
/ 7,58
I 25 0
5______
/ 7 96
/ 7 58
/ 25 0
Growth
Fl
100
>100
>100
Dunnett's
Survival
Fln
100
>100
>100
Steel's
Overall Result
ChV | >100
0
8.17 / 7 97
7.45 / 7.20
25 8 / 25.0
0
8 24 / 8 10
741 / 7 29
25.8 / 25.0
Day
3
8.22 /
7 50 /
25.1 /
Growth
Critical Calculated
2.41 -0.1594
2.41 -1 4254
2.41 -0.7221
2.41 -0.2298
2.41 -0 9987
1
8 28 / 8 01
7 52 / 7.46
25.2 / 24.5
I
Facility:
NPDES #:Outfall: rJQ /
Date of Test:
by: /fa Organism Pimephales promelasIFinal weights taken on: -/'7 by:
I
CONC REP # ORG.SURV %
776^8A ^<<35"io /DO
B 7-STS ^■4^7IO IDOI
707.37 g^, 30c O<3^7to iOO
D %(O DO
I gP3.7-0A • 766'D 100
0,oZ B IO o. jry (&0
773cro |O0
%D 0^3712S.loO
2% ,70 ^«Z2A Io \0DI\.o/7A3.U)"792,^7 Io6>77B 0-633 |bO
790,^c 79-76 9.a2 6M'Q2 IOO
7/-Z-2%uiD
I
i
Page: J_of 2
‘/■73
2/7'f
PAN WT.
(mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
PAN + ORG.
WT. (mg)
SOG .52.
MEAN
WT./ORG. (mg)
c
0
xr
V
I
I
-----------------
Me oo z.7 9
G-Vxvji
sVIERITECH, SSMC.
ENVIRONMENTAL LABORATORIES
A rji^inn of l.V.nrc., T<..chr.o/aay .--.Ac/ Confro/i /nc
0
L
Initial weights taken on:
3^
WT. OF ORG.
(mg) !
S7D3
I
I Facility:Page: 'Z-of 2
Outfall: OC/
Date of Test:
Organism: Pimephaies promelas
by:
I
CONG REP # ORG.SURV %I A
1.0 7.77 iB 4 Id I CDo. H- W
^.02) c IO | Co
743DI /0 o .4-76 I CD
A io luO■1 o
^.0'7 ?07.57B 7.-10 0^3 100
itc 1'0y.?7
I ?0/, 3D ID 69.DO
^88 72A 0.^35^4.3 ID 100
8.0 7.7%.C\B op .7k ID 100
(g-03'01.c 3iO iDO
776.0^D 10D10
I
t
^.70
TtLSfe
I
PAN WT.
<mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF ORG.
(mg)
MEAN
WT./ORG. (mg)
PAN + ORG.
1 WT. (mg)
m2.
BV3ERBTE^Hr
ENVIRONMENTAL LABORATORIES
A rji^icn Of Mm,, T^hnotoe/y .mu Cun,^. ,„c
Final weights taken on:
174% .PS
74G. 31
-OlavjfVotA
NPDES #: Ajo
7 !
Initial weights taken on:_ ?/j^/cl by:
^17-h
ML
0^6^
I
Type of Facility Generating Effluent:jn
I i.
I

Shipping Information
I Date: 6b/(5~? /C>7
Sample Condition:
Relinquished By:
Received By:
Relinquished By>^
Received By:
Sample Temperature (C°):
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:START
END
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements:
Test Required: Chronic:
Acute:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]

Toxicity- Test Information
£ -P-P Z\^c=‘.
Grab
Date:
Date: _
Sample Chilled (Covered with Ice): Yes
Collector’s Name (Print):
Collector’s Signature:
Time: PM
Time: pm
Time: AM^M>
a./'^ r~
__ Composite
Time: _
^-20^ Time:_
No___
Date: S'
Date:
Date: &
Date:
Method of Shipment!
Circle One
/7/o
Time: fl IA5AM PM
AM PM
^(^/u)) UPS Fed-Ex
‘tycri/<!■}
# of Containers
fl-‘3 6 £53 PM
/O.’OO PM

Client Information
Client: V ty c -F OAct^ V'O PO#
Contact: —NPDES #: 71/
Address: ^7 7 Phone: (g
C’ty- —d- /-n_____________________________ Pipe Number: /
County: ^AnS State: A/.C^ Zip: ^75^^
Sample Receiving (Laboratory Use Only)
Received From:
Received By:
Sample TemperatZ/e (C°): 0^ /
I
57*-
Zip:
Type of Facility Generating Effluent:
I
Toxicity Test Information
£> °/G

Shipping Information
Date:
I
I
AM PM
Relinquished By:/? f/a
Received By:
Relinquished
Received By:_
Sample Temperature (C°):
Sample Informatio
Sampling Site: _
Sample Type:
Sampling Time:
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
IWC:________________
Toxicity Test Concentration(s):
List Any Special Requirements:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
Date: ^-<7
Sample Condition:
—-
Sample Receiving (Laboratory Use Only)
Received From: C o yyjoS
Received By?^
Sample Temperature (C°): U 7T?
Time:1 30
T c c
cS*Q U<f> /
Composite
-Of' Time:
Time:
Z No
jcMDate: Time: ///STZaSi PM
_ Date: ^^2 7 Time: // 5^ AM PM
- Date: Time: AM PM
- Date:Time:AM PM
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
# of Containers J2_
/g/oO @^PM
(AM>M
PO#
NPDES #:
Phone:
Pipe Number: 0c> f
^7^5^
n
________ Grab___
START Date: _£
END Date:
Sample Chilled {Covered with Ice)'. Yes
Collector’s Name {Print)'.
Collector’s Signature:
Client Information
Client: Toma'F
Contact: e o_ ______ ____
Address: p.g, / /OgQ
City: Z
County: State:
Test Required: Chronic:
Acute:
I
£□4
Zip: I Jx LOLdTpType of Facility Generating Effluent:
1
I
Toxicity Test Information
^2---
Shipping Information
I
Time:Received By:
Sample Tempera
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:START
END
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]

Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements:
Client Information
Client: a
Contact:
Address:
City:
County:
(C°): 0.S7Q.S-
Sample Receiving (Laboratory Use Only)
Received From: (A
Date:
Sample Condition:
# of Containers
PM
pm
POU
NPDES #:
Phone: (g
Pipe Number: /
-C !
Grab
Date: _
Date: _
Sample Chilled {Covered with Ice): Yes
Collector’s Name {Print):i
Collector’s Signature:
i^Date: Time: /.'ANwZ
1 Date: Time: AM^p
— Date: Time: X^aM gNp
Date:Time:AM PM
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
/a-f
"J4*rte.s (4} re-K
diy 'To o____
Relinquished By:
Received By: QjLt
Relinquished
Received By:
Sample Temperature (C°):
Composite
O 7 Ti me :
T' ~/0 -o 7 Time:
No
Test Required: Chronic:
Acute:
# of Organisms per Chamber: 10 Start: Date:Client:
Test Vessel Size: 400 ml End: Date:
Test Solution Volume: 250 mlInitiated by:
Temp, of Stock: Date/Time Fed: Randomization:No
# of Reps: 4Date/Time Born:Incubator #:

Transferred by: Fed by:
TimeDate Initials Date Time 1 Initials Time 2 Initials Time 3 Initial
w\-Day 0Day 1
Day 2 Day 1
7'3ftDay 3 Day 2
Day 4 Day 3
S'30^Day 4 Day 5
Day 6 Day 5
Day 6
Test Termination Data:
Initials/Signature:
End Date:
End Time:
/o ?
g/?/o 7
lo-tO^v
7.-dV
NPDES#: NC
____
°C
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
g/z0
^3

*
= Weekend, only 2 feedings needed
y|^Ol
<4;lbr-
pit- '/P
Hu-
Time: *[
Time:
es
Concentration: control 3 5 7 Remarks
PH
D.O.
1 Temp.
Conductivity
3 Remarks
D.O.
Temp.
Conductivity
5 Remarks
D.O.
Temp.I
Conductivity
Day
4
Time:
Time:
Concentration: [ Q /z
pH_______Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
5Concentration:
pH___________Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine I
____7
287
Batch #
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
_______Hardness
Alkalinity
20^
i*
I
/
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1
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7-7'/
3
211L
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257
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7.9 (
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730
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270
£0. I
7,0^
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((^
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203
240
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IVIERSTECH, gf^C.
ENVIRONMENTAL LABORATORIES
Division c>f Water Technology and Controls, Inc.
Chemical and Physical Determinations
Client: C-laq-h^_______ NPDES#: oc om.C'i'S'D Start Date: Kh/c. i
Test Organism: Pimephaies promeias Analyst(s): End Date: $7,4/0 ?
r" r --------------------------
I
Chemical and Physical Determinations
Remarks
D.O.
Temp.
Conductivity
Remarks
D.O.
Temp.
Conductivity
Remarks
D.O.
I Temp,
Conductivity
2 6 7 Remarks
Day
4
ENVIRONMENTAL LABORATORIES
XI O-v,s/on Controlt. /r>c
Concentration: 100%
__________________pH
__________Conductivity
_____Residual Chlorine
____________Hardness
___________Alkalinity
Concentration:
PH
Concentration: X
pH__________Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
___1
XJ?
7,9^
V r
7/ yp
£C>I
3
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Day
4
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27 3
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^5
I
7.nz
ZQx
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27.
7, c I ->
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237
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2,2^
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Initial
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Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
3
7^0
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29/
5
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2X1
7 ,? 6
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6
76.ri7.7i
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<73 i
281
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7.^7
753
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7,11
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7
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7.7,3
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230
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Client: C la __________
Test Organisirt: Pimephales promelas
Concentration: 2,0/,
pH 7__Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
Page 2 of 2
Time: c/'
Time: ^.ZC^5—
5
E/S"
■Tg
13 i
7.C2
ZsZo
2^3
6
3.^
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7.^6
7.7$'"
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317
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NPDES#: M c 66 z.SVdT3 Start Date: ^7*7 /o -
Analyst(s): /HA-End Date: /c 7
7
_^01
■7^3
^77
'7zS"2
233.
^7^
£.0 ■ /
C 7 Start Time:Start Date:7Client:
End Date:
Concentration
Day 7Day 6Day 5Day 4Day 3Day 2Day 1Day 0I co o oRep#1
0 ooooooRep #2
0 ooQooo Rep #3
0 oooooooRep #4
0^5 7^Concentration
Day 6 Day 7Day 5Day 4Day 3Day 2Day 1Day 0
o0o6OoOoRep#1
0 o00ooooRep #2
&O0ooo.oRep #3
6ooo0o0r>Rep #4
Concentration
Day 6 Day 7Day 5Day 4Day 2 Day 3Day 1Day 0
0 Ooo ooRep#1 O
0 0oo0£oRep #2
0 0o ooRep #3
0 00 o ooVRep #4
ckk1
ivit_r\j i uvi i, nwr.
Mortality Data: Chronic Fathead Test
Hl 033^
7V /t17 End Time: —
I
Client:
End Time:End Date:
Concentration J? ? Q A-
Day 7Day 6Day 5Day 4Day 3Day 2Day 1Day 0
0O0oRep#1
000OoooRep #2
0OOOOoRep #3
0 OOo0onRep #4
7.0 xConcentration
Day 7Day 6Day 5Day 4Day 3Day 2Day 1Day 0
0OOOOooRep #1
0OO0C?O ORep #2
0ooc>oRep #3
OOoooRep #4
Concentration
Day 6Day 5Day 4Day 3Day 2Day 1Day 0
00oooRep #1
OOo6OoRep #2
000oooRep #3 o
0 000 oooRep #4
ivicrxi i i, iimvs.
Mortality Data: Chronic Fathead Test
Day 7
0
M’.Start Date:, 7 Start Time:d-\,a.vx4r-TA
Kj
Transform:NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
Data PASS normality test (alpha = 0.01).Continue analysis.
I
D =
W =
0 . 1023
0.9530
Title:
File :
Critical W
W
= 0.8840 (alpha = 0.01 , N = 24)
= 0.9160 (alpha = 0.05 , N = 24)
clayton
clayton
Transform:NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated Bl statistic =(p-value = 0.0798)9.8433
Data PASS Bl homogeneity test at 0.01 level. Continue analysis.
Critical B
I
Title:
File :
(alpha = 0.01,
(alpha = 0.05,
5)
5)
= 15.0863
= 11.0705
df =
df =
clayton
clayton
Transform:NO TRANSFORMATION
ANOVA Table
SOURCE DF SS MS F
Between 5 0.0176 0.0035 0.6183
Within (Error)18 0.1023 0.0057
Total 23 0.1199
(p-value = 0.6876)
Critical F
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)
I
Title:
File :
= 4.2479
= 2.7729
(alpha = 0.01,
(alpha = 0.05,
df = 5,18)
df = 5,18)
clayton
clayton
Transform:NO TRANSFORMATION
Dunnett's Test Ho:ControlcTreatmentTABLE 1 OF 2
GROUP IDENTIFICATION T STAT
Dunnett critical value = 2.4100 (1 Tailed, alpha = 0.05,df = 5,18)
Transform:NO TRANSFORMATION
Dunnett's Test TABLE 2 OF 2 Ho:ControlcTreatment
GROUP IDENTIFICATION
I
I
Title:
File :
Title:
File:
MEAN CALCULATED IN
ORIGINAL UNITS
SIG
0.05
1
2
3
4
5
6
NUM OF
REPS
TRANSFORMED
MEAN
4
4
4
4
4
4
0.4390
0.4475
0.5150
0.4775
0.4513
0.4923
MIN SIG DIFF
(IN ORIG. UNITS)
0.1285
0.1285
0.1285
0.1285
0.1285
0.4390
0.4475
0.5150
0.4775
0.4513
0.4923
% OF
CONTROL
29.3
29.3
29.3
29.3
29.3
DIFFERENCE
FROM CONTROL
-0.1594
-1.4254
-0.7221
-0.2298
-0.9987
-0.0085
-0.0760
-0.0385
-0.0122
-0.0532
1
2
3
4
5
6
control
. 5
1
2
4
8
control
.5
1
2
4
8
clayton
clayton
clayton
clayton
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date 9/21/2007
Facility Town of Clayton NPDES # NC0025453 Pipe#' 001 County Johnston
Laboratory Meritec Comments
I
MAIL ORIGINAL TO:
Test Initiation Date/Time 9/11/2007 I 3:00 PM Avg Wt/Surv. Control [0.513|Test Organisms
% Survival [100 0|
Avg Wt (mg)[0.513|Hatch Date 9/10/07
100 0|% Survival [Hatch Time 3 pm CT
0.505|
100 0|
0 465|Avg Wt (mg)f
% Survival f 100.0|
Avg Wt (mg)[0.505|I % Survival [100.0|
Avg Wt(mg)[ 0 4671
% Survival [100.0|
0.500|Avg Wt (mg)[
I
I DWQ Form AT-5 (1/04)
1
9/10/2007
10
10
0 468
10
10
0.513
10
10
0 486
10
10
0 421
10
10
0 438
10
10
0 477
7.77
6 85
25 0
10
10
0.396
10
10
0.529
Avg Wt (mg)[
% Survival
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp, at Receipt (°C)
Dilution H2O Batch
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
High Concentration
pH (SU) IniVFin
DO (mg/L) IniVFin
Temp (C) IniVFin
8| Surviving #
Original #
WVoriginal (mg)
2| Surviving #
Original #
WVoriginal (mg)
4| Surviving #
Original #
WVoriginal (mg)
11 Surviving #
Original #
WVoriginal (mg)
0 5| Surviving #
Original #
WVoriginal (mg)
Growth
p|
Fl
Cultured In-House
Outside Supplier
Normal
Hom Var.
NOEC
LOEC
ChV
Method
Environmental Sciences Branch
Division of Water Quality
NC DE NR
1621 Mail Service Center
Raleigh, NC 27699-1621
180
4 00
45.00
192
10
10
0.500
10
10
0.521
10
10
0 487
10
10
0 538
10
10
0.442
8 01
7 52
25.0
181
42
55
194
2
9/11/2007
10
10
0 494
10
10
0.496
10
10
0 483
2
10
10
0.509
8 15
7 84
24 8
3
9/13/2007
10
10
0.437
10
10
0 476
8.06
7 64
25 0
8.14
7.90
25.1
8.20
7 74
25 1
183
40
53
196
10
10
0 547
10
10
0620
3_____
/ 7.89
/ 7.07
/ 25.1
8.00
25.1
Survival
Critical
10
10
10
10
10
Calculated
18
18
18
18
18
7.93
7 65
25 0
1
10
10
0464
23,00
60.00
180.00
1112
<0.1
1.4
7.90
7 41
25 0
182
40
50
187
3
10
10
0 477
4
10
10
0.601
Stats
Cone.
0.5
1
2
4
8
23.00
58 00
178 00
1130
<0.1
09
23 50
60 00
178.00
1140
<0 1
1.3
8
>8
>8
Steel's
8
>8
>8
Dunnett's
% Eff. Repl.
| Control | Surviving #
Original #
WVoriginal (mg)
1
8 14 /
7,64 /
24 8 /
______0
8.07 /
7.69 /
25.0 /
______4
8 15 /
7 92 /
25 1 /
Water Quality Data
■ Control
£ pH (SU) IniVFin
DO (mg/L) IniVFin
Temp (C) IniVFin
Survival
ri n
______2
8.02 /
7 90 /
24 9 /
1
8.11 / 786
7.65 / 7.36
24 8 / 25.0
0
8.18 / 8.04
7 58 / 7 46
25.1 / 25.0
2_____
/ 7 98
I 7.70
/ 25.0
Growth
Critical Calculated
2.41 0 1963
2 41 1.2564
2.41 0 1963
2.41 1 1909
2.41 0.3403
5
8.12 / 7.96
8.09 / 7.15
25.0 / 25 1
______6
8 16 /
7 82 /
25.0 /
5
8.12 / 7.84
7 94 / 7.12
25 0 / 25 1
6
8 18 / 8.00
7 78 / 7.65
25.0 / 25.0
4
8.19 / 795
/ 7 46
/ 25 0
IC_______________
*_________
Signature of Op^ralOMfi Responsible Charge
x_____________________
Signature of Laboratory Supervisor
Day
3
/ 8.06
/ 7.21
/ 25.1
Overall Result
ChV | >8
Start: Date:# of Organisms per Chamber: 10
End: Date:Time: Test Vessel Size: 400 ml
Test Solution Volume: 250 ml
NoRandomization:Date/Time Fed:
Incubator #: Date/Time Born:# of Reps: 4
Fed by:Transferred by:
InitialTime 2 Initials Time 3DateTimeInitialsDateTime 1 Initials
4^4 ^41 A'—W1LDay 1 Day 0
^14 C- Day 2 Day 1
Z//A-IHLtil L ^\r'L

A-Day 3 Day 2
Day 4 Day 3 I
I -3^,1^-ML- Day 5 Day 4
f3^fa' Day 6 Day 5 /ViL
nil Day 6
Test Termination Data:
Initials/Signature:
End Date:
/ry:3^End Time:
9 ////d
8'70'7
r.^
°C
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Initiated by: /Hl,
I/O/o'.
7
*
= Weekend, only 2 feedings needed
/I! CO^
US
Vite rtf thfl-
Client:
NPDES#: NC
Time:
Temp. of Stock: 3
2 6 Remarks
I pH
D.O.
Temp.I
Conductivity
I
I Remarks
D.O.
I Temp.
I Conductivity
Remarks
D.O.I
Temp.
I Conductivity
I
Time:
Time:
NPDES#: OOZS4S3
Analyst(s) Mf
Concentration: l,(y
pH_______Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
Concentration:
PH
Concentration: control
Batch #
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
________Hardness
Alkalinity
2.5.0
^-o • \
8'11
rl^
3
a
zo<lz7.fc4
7.70
2-4.8.
~ :>,4
Day
4
6
8.1°
1.90
'OV-'
ZAL
2^.0
Z-K. I
113,
XL<2>. I
6
g./b
2AL
7.87
ij'.o
zr.i
209
ya.1-/
Z<5- I
____7
-1Q
n.Qu
-7.ro
7.
■ZS'.O
zr.b
113
3
g. II7.^
'1.80
Zb? .
ir-i)
l<}Q
z.o.|
202
i-O. I
6 , i z-
7.67
7.?‘7
7,i^
Z a'- <7
21.1
|gy
I
O, 5 -/■
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
2
g/0
7.B8
7.70
'1.^3
3
8.15
7.91
7.Sto
75'9
ZS.O
Zo. |
1
8,15
7.77
7-57
7-71/
2,5-1
IM 7
<0-1
Day
4
7.03
?-5. )
AO. /
7
8 10
■is. 8
is. 0
Ilf)
378
^0. (
1
e.K
g’-QO
7.66
7 My
^5,1
ZJ7t>
^0
^■o. I
5
_£ZZ_
7.^ “7
n.ea
7,4^
zr.t)
zoo
<6 J
5
777
zr.o
ZOM
</?</
so
1
I Ku
7. SB
7.76
25. |
ZS.O
Xc4
^•o.l
HQ
MS
_____7
163
• I 93
7. L5
2^0
2^0
y
Zc>. I
2
8,/0
7. 8H
2.69
7- 45
zy.r
Xbtf i
40. /
Day
4
876
7.^3w
2^
z.d-1
5
-7, ay
8 ,qo
6 fig
o
i-SO
n?
.1
7,3(0
/ 77
I
Client: C.'Vq.sX'YoA_______
Test Organism: Pimephales promelas
Chemical and Physical Determinations
Start Date: 9- 11 -()/l
End Date: V/y/cO~
e.i Y
7.9Q
9/2.\
2£\|
>5" 4.
/7M
ZIC)
2-0. |
I Chemical and Physical Determinations
Remarks
D.O.
I Temp.
Conductivity
»^6 Remarks
D.O.
Temp.
Conductivity
Remarks
D.O.
Temp,
I Conductivity
I
2 6 7 Remarks
I
Concentration:
PH
Day
4
HSIEFtfrECH' me.
FNVIRONMENTAL LADORATORIFS
Page 2 of 2
Time:
Time:
Concentration: 100%
____________________PH
___________Conductivity
Residual Chlorine
_____________Hardness
Alkalinity
Concentration:
pH
Concentration: 2,0 7-
pH_______Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
6.0 Z
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
%O7-
Initial
Final
Initial
Final
Initial
Final
Initial
Final
Residual Chlorine
1
1130
5b
10^
3
7M3
33 &
zo. )
5
'7, £3
7, II?
2^.0
34
5
Kmc
Z6. I
(oO
i
b.cq
JM
7. cq
7.52,
ZS . o
o. I
6
R.12-
_2rlk_
?./<
ZJ70
3
///a
<0- I
GO
5
S-17
7,8^
7.Q4
74Q
2570
36 fr
371
< z> J
3
ft, 08
7.95"
•IMO
7.57
24 m
20^
7/^7
^o. 1
7
8, 00
7.7%
7.05
_L£LO_
TSZO
^0
^O.l
2
7.4 b
7.67
7. 5 6
25.0
p/3
C. Q • )
_____7
8 /b
7.77
-? 7 o
2^
i
K lb
7.9 7
766
7.3 7
25 J
25'-o
_2k3_
Day
4
R./6 zTr
7.80
7.02.
I
7^5
^o- I
->•<4
23^
ZHb
xD-/
2
fti*/
7.96
7fc7
7.41
•2-M.r
25.0
4C>.|
2
fe.W
^.93
7.44
25.0
3-ivr
33/
1
Day
4
8?-O
7^
777
7.07
2.r, I
2T2_ w~
Zd)j
5
v/9
aoo
7.96
Zf.O
20 r| 7JO
2f\o
6
^.|7-
-2MC(
7.^.0
7.-5.1
131
I
3
hMZ
8- 06
7.A0
769
29.9
177
7^8
/<?. i
i
4,
76/0
25-u
•Z5-.0
X O . \
________ 7
^£'4 6./¥
A. oi
7.74
•7. v-r
■Z.57O
'Z&'.D
'X7Q Tv. /
Client: ___________ NPDES#: A;C 00^5^53 Start Date: 3-//-07
I est Organism: Pimephaies promelas Analvst(s): AU., A46. End Date: // ^/o ~)
Day
4
6.17
202-
g.ag
25-. /
Zb |
7/8
Zo- 1
MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Start Date:
I End Time: ID’3^—End Date:
CcfthxIConcentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
D OoRep #1 O O C)Co
oo(JRep #2 0 oooCl
oo0 aRep #3 o ono
o 6 o C)Rep #4 0 O o
%I Concentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
o oRep #1 O oo£2 o
Rep #2 O ooo 0oo o
Rep #3 C o oo o ono
6 0Rep #4 0C)o £)£>
Concentration /.O
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
Rep #1 oo o o
bbRep #2 o 0oo oI0oRep #3 O o 0 o0
00Rep #4 0a o a
I
I
V/7 A?Start Time: gcUopTvrt
I
Client:
I !o~) End Time: 1^' 36^^
I 2.0 7.Concentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
o0oc>oo6 ORep #1
O o ooRep #2 O 0o
o o oRep #3 O oo oo
00O oRep #4 Q O oC)
H^O 7Concentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
OO ORep #1 OC> O o
oo C)Rep #2 o oIo oo
0o0o 0Rep #3 o o o
000oRep #4 0 o o
fi.o zConcentration
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7
O d)Rep #1 o 0 o oo
0 oc>Rep #2 O 0o &
0 o00Rep #3 O o o
6 o0 6Rep #4 <5_0_
MERITECH, INC.
Mortality Data: Chronic Fathead Test
/End Date: /(
Start Date: T/p l(>~) Start Time:
of 2Facility. I NPDES #:Outfall: 60/
Date of Test:
by: Organism: Pimephales promelas
by: AVC-
I
CONG REP # ORG.SURV %
464702,76 /O /uoA
776^7B ID o.-So?I Du
7 ?c O-V77ID I OU
'771.30 6.01D (0 >.(pOI ioo
77S7^&HfiO.OOA IO O'WU I QU
6*^ 7--l.b'i77/77b. 34B ZD I(jO
7R8A1 <7Gc /t>oO.47G!o
Ip. ZO7^1./ID /O 0.G2O /Ou
7g 3 ^7 ^.8778^.36A IO /oo
77 ?-/gri.oz 772.31 U3B to 0.^13 /DO
4.217$^.c ID 0.^7)I Ou
lom 73D IbO1
Page:
Initial weights taken on:
Final weights taken on: 4-20~O7
PAN WT.
(rng)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF ORG.
(mg)
MEAN
WT./ORG. (mg)
PAN + ORG.
WT. (mg)
MC.
ENVIRONMENTAL LABORATORIES
DA’.i/on o/ lA'.iirez- Zedno/CQi- ^ncJ Controls Inc
G
O
.V
T
IT
o
L
/u c Qo 2- s~ r7G»
^///07
Page: Z- of 2Facility:I Outfall: QG INPDES#:
Date of Test:
by: Organism: Pimephales promelas
by: ML
I
CONG REP # ORG.SURV %
7?7.zo 5.IoA I Ob
7^4.??7^/O' 7££>B l()OIO
4.%785.4/2^5c (2-L/Bb/Q (DO
5.z°iW3.lctD /ooZ£2
797.04 79Z.M 5. QC oSooA /DO/O
-?77.3y 7^4.3o 4.9 C9B I (J 0.4?^/COH.o z
7^6.3^790.34 3.9kc IO Q-Z7b loO
4.7778/,/37 70- 36D /o ODT?zoo
5.21A OS'Z/|0 loo
4.948.0 7.793.88B IO /Of)
7?/. 7 7 495.64 4.39c ID /Oo
W.4/5.47 ^<7?791.88 IoD loo
PAN WT.
(mg)
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
WT. OF ORG.
(mg)
MEAN
WT./ORG. (mg)
PAN + ORG.
WT. (mg)
4-G8
2g3, ZG 788.84
tq^4r>A___________
fi)C 00 7.5*4^3
7// Zi ?
Initial weights taken on:
Final weights taken on: 4-26-Of
ENVIRONMENTAL LABORATORIES
’A On »siozi of I'i'jirer TfchnoloQy .incl Conirols inc
I

o^0
I
Zip:
Type of Facility Generating Effluent:
Toxicity Test Information
Test Required:
I
p e.
Shipping Information
I
Received From:
Sample Receiving (Laboratory Use Only)
Relinquished By^
Received By:
Relinquished
Received By:

Sample Temperature (C°):
Sample Information
Sampling Site:
Sample Type:
Sampling Time:
Chronic:
Acute:
Test Organism: Ceriodaphnia Dubia: X
Pimephales promelas:
Mysidopsis bahia:
Received By: / \ -------"
Sample TemperaWe^C0): Q 5 IQ
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements:
•^6 /'y
2 %
__TTdx/c / A- 4
/i no t.j
Composite
Time:
Time:
No_
txJ oc-r~/*"<
'P.O. 6 OX 5^7 7
cg-r
Grab
Date:
Date: o 7
# of Containers &
<XnT) p*m
to.'QO A05 PM
z_
y 7^- O 7
Client Information
Client: otj k. o 'G
Contact. e. 3
Address:
City:
County:
/ / a o T\ t'Lf l\ a
State:
Date: ?////o ?
Date:
__ Date:
__ Date:_________
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
START
END
Sample Chilled {Covered with Ice): 'Yes,
Collector’s Name {Print):
Collector’s Signature:
Time: //^Q ApPM
Time:W PM
Time: AM PM
Time:AM PM
Date: __Time
Sample Condition: [ £
PO#________________
NPDES #:
Phone: if)
Pipe Number: /
<r
I
State: Zip:
I
I
Toxicity Test Information
I
I
I
'• I @>am(pm)
Collector’s Name {Print)-.
Collector’s Signature:
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:
Test Required: Chronic: )(
Acute:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
Test Organism: Ceriodaphnia Dubia:
Pimephales promelas:
Mysidopsis bahia:
Relinquished By:
Received By^2*h>vZ^^
Re 1 inquisheoBy:
Received By:
Sample Temperature (C°):
^3.
/^r__________
# of Containers ;
<SPPM
O PM

Shipping Information
ffA- Date: //1
Date: ^/v 'Lyp '
Time: / O
Time: PM
Time: AM(gKp
Date:________ Time:AM PM
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
Sample Receiving (Laboratory Use Only)
Received From: “7
Received By:
Sample Temperature (C°): (, y (t 7
A-
Composite
Time:
Time:
No
6 mZ
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements:
—y On 'Be >4^
ke-a-oIL isf't t k HC) Vil •______
U A
Date: /1 \ l(jj Time: D ‘
Sample ^ond/tion:
___ PO#
NPDES #:
Phone: /7 ) ^<5
_ Pipe Number: f
S'
e_ r cc.»-v
______-f-f /
Grab X
START Date: 5//z/<? 7
END Date:
Sample Chilled {Covered with Ice)-. Yes X
Client Information
Client: "T^uJh. q ay<4'o n__________
Contact: ttfa/reXT ______ __
Address: 'P.O.Qq y 5 //^QO Durkan
City: (5/ <x-<r 0^7
County:
zr*7 5 •/-©
Type of Facility Generating Effluent:
I
I
Zip:
Type of Facility Generating Effluent:
Toxicity Test Information
I
I
I
Shipping Information
AM
Sample Information
Sampling Site: _
Sample Type:
Sampling Time:
Test Organism: Ceriodaphnia Dubia: X
Pimephales promelas: X
Mysidopsis bahia:
Meritech, Inc.
Bioassay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
[phone]
y/gdz SC-
# of Containers
0^30
_ __5
.(g&PPM
/AM PM
Date:
Sample Condition:
tdet—Tf' g. m_____
Q j)
IWC:
Toxicity Test Concentration(s):
List Any Special Requirements:
<?//^^) 7 Time: AM^KP
Zc/X^> Tirne: /^Zj'AM PM
V -/ Time: c3Z5-^A.M<^^-
AM PM
Date:
Date:
Date: V^-/ ty/yfry Time:
Date:£ Time:...
Method of Shipment: WTC(p/u) UPS Fed-Ex
Circle One Other:
Relinquished By:
Received By:x^
Relinquisheo By;
Received By:
Sample Temperature (C°):
Received By:
Sample Tem^
__________PO#_________________
________ NPDES #: 4/
Phone: -
Pipe Number: p/
4g
Set /-e-r
X Composite
Time: _
Time:
No____
gy> /•. ~7tf>
Ao zZL
Client Information
Client: h o -f
Contact: -7^^ gs
Address: ?O Bay.
City:___
County: ■^a>h s o
lime:
•Trccl
I *
Grab _
START Date:
END Date: -^00^
Sample Chilled {Covered with Ice)'. Yes
Collector’s Name {Print)-.r 4
Collector’s Signature:
Sample Receiving (Laboratory Use Only)
Received From:
?ure(C°):/< 3 TTS
Test Required: Chronic:
Acute:
Transform:NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
N = 24)
I
Data PASS normality test (alpha = 0.01). Continue analysis.
I
I
I
I
I
Title:
File:
D =
W =
0.0525
0.9566
Critical W
W
= 0.8840 (alpha = 0.01 ,
= 0.9160 (alpha = 0.05 , N = 24)
Clayton
clayton
Transform:NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated Bl statistic =(p-value = 0.7958)2.3712
Data PASS Bl homogeneity test at 0.01 level. Continue analysis.
Critical B
I
I
I
I
Title:
File :
(alpha = 0.01,
(alpha = 0.05,
df =
df =
5)
5)
= 15.0863
= 11.0705
Clayton
clayton
Transform:NO TRANSFORMATION
ANOVA Table
SOURCE DF SS MS F
Between 5 0.0088 0.0018 0.6016
Within (Error)18 0.0525 0.0029
Total 23 0.0613
(p-value = 0.6995)
Critical F
Since Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)
I
= 4.2479
= 2.7729
(alpha = 0.01,
(alpha = 0.05,
df = 5,18)
df = 5,18)
Title:
File :
Clayton
clayton
F <
Transform:NO TRANSFORMATION
Dunnett's Test TABLE 1 OF 2 Ho:Control<Treatment
GROUP IDENTIFICATION T STAT
Dunnett critical value = 2.4100 (1 Tailed,alpha = 0.05,df 5,18)
Transform:NO TRANSFORMATION
Dunnett's Test TABLE 2 OF 2 Ho:Control<Treatment
GROUP IDENTIFICATION
I
Title:
File :
Title:
File:
1
2
3
4
5
6
NUM OF
REPS
TRANSFORMED
MEAN
4
4
4
4
4
4
0.5127
0.5053
0.4648
0.5053
0.4673
0.4997
MIN SIG DIFF
(IN ORIG. UNITS)
0.0921
0.0921
0.0921
0.0921
0.0921
MEAN CALCULATED IN
ORIGINAL UNITS
0.5127
0.5053
0.4648
0.5053
0.4673
0.4997
% OF
CONTROL
18.0
18.0
18.0
18.0
18.0
DIFFERENCE
FROM CONTROL
0.1963
1.2564
0.1963
1.1909
0.3403
0.0075
0.0480
0.0075
0.0455
0.0130
SIG
0.05
control
. 5
1
2
4
8
2
3
4
5
6
control
. 5
1
2
4
8
Clayton
clayton
Clayton
clayton
1
I
I
Sludge Management Plan
Sludge Management Plan
I
I
I
I
I
J
>sal as discussed below, j id.
RF are dispose^rof off-site by a contract hauler. East Coast Resources,
ijy q(WQ0Q00506, is the primary hauler and Granville Farms, operating
under Non-Discharge Permit No. WQ00048gMs a backup hauler. Both sludge haulers require that the Town
prepare the biosolids to meet Class B pathogen requirements, as discussed above, prior to removal from the
LCWRF. East Coast Resources and Granville Farms meet the vector attraction reduction requirements by
employing Option 9 [40 CFR 503.33(b)(9)] and/or Option 10 [40 CFR 503.33(b)( 10)]. Option 9 requires
injection of the sewage sludge below the land surface. Option 10 requires that the sewage sludge applied to the
disposal site be incorporated into the soil within 6 hours after application.
A third hauler, McGill Environmental, operating under Non-Discharge Permit No. WQ0006816, does not
require the Town to meet the Class B requirements prior to removing sludge from the LCWRF. McGill
Environmental dewaters the sludge at the plant then hauls the sludge to their composting facilities for further
processing to meet Class A pathogen and vector attraction reduction requirements prior to disposal. The pathogen
reduction requirement is met through Alternative 1 [40 CFR 503.32(a)(3)-(8)], monitoring of fecal coliform,
and/or Alternative 5 [40 CFR 503.32(a)(7)], composting PFRP. The vector attraction reduction requirement is
met utilizing Option 5 [40 CFR 503.33(b)(5)]. Option 5 requires the sewage sludge to be aerobically treated for at
least 14 days at over 40° C with an average temperature over 45° C.
C
I
Biosolids Preparation-.
W aste Sludge from the BNR type activated sludge system is thickened in the rotary drum thickeners and then
transferred to the existing 90,000 gallon and 360,000 gallon aerobic stabilization / storage tanks and the new
360,000 gallon aerobic stabilization / storage tank. The underflow from the sludge thickener is returned to the
oxidation ditches. Once stabilized, the sludge is hauled away by a contractor for off-site disposal. Decants from
the sludge stabilization / storage tanks are recycled back to the WAS pump station.
Biosolids generated by the LCWRF are prepared to meet Class B pathogen requirements through Alternative
1 [40 CFR 503.32(b)(2)] and Alternative 2 [40 CFR 503.32(b)(3)]. Alternative 1 requires that the geometric
mean of fecal Coliform density, in seven samples of treated sewage sludge, not exceed 2 million Colony Forming
Units (CFU) or Most Probable Number (MPN) per gram of sewage sludge solids. Alternative 2 requires that the
sludge be treated in a process to significantly reduce pathogens. The process currently employed by the LCWRF
is aerobic digestion. The vector attraction reduction reqwrementsjire satis^. byJhe sludge hauler(s), not by the
LCWRF, prior to dispo^l as discussed below, j nchri
Biosolids Disposal: i
Biosolids generated' by the LCW1
operating under Non-Discharge Permi
Little Creek Water Reclamation Facility
NPDES Permit No. NC0025453
Town of Clayton, North Carolina