NC0084387_NC0084387 - Renewal (Application)_20240904

ROY COOPER
Governor
ELIZABETH S. BISER
Secretary
RICHARD E. ROGERS, JR.
Director
William Deal
Charlotte Water
5100 Brookshire Blvd
Charlotte, NC 28216-3371
Subject: Permit Renewal
Application No. NCO084387
Lee S. Dukes WTP
Mecklenburg County
Dear Applicant:
NORTH CAROLINA
Environmental Quality
September 04, 2024
The Water Quality Permitting Section acknowledges the September 4, 2024, receipt of your permit renewal application
and supporting documentation. Your application will be assigned to a permit writer within the Section's NPDES WW
permitting branch. Per G.S. 150E-3 your current permit does not expire until permit decision on the application is made.
Continuation of the current permit is contingent on timely and sufficient application for renewal of the current permit. The
permit writer will contact you if additional information is required to complete your permit renewal. Please respond in a
timely manner to requests for additional information necessary to allow a complete review of the application and renewal
of the permit.
Information regarding the status of your renewal application can be found online using the Department of Environmental
Quality's Environmental Application Tracker at:
https://www.deg.nc.Rov/permits-rules/environmental-application-tracker
If you have any additional questions about the permit, please contact the primary reviewer of the application using the
links available within the Application Tracker.
ec: WQPS Laserfiche File w/application
D Q-
Since
Cynthia Demery
Administrative Assistant
Water Quality Permitting Section
North Carolina Department of Environmental Quality I Division of Water Resources
Mooresville Regional Office 1 610 East Center Avenue, Suite 301 1 Mooresville. North Carolina 28115
704.663.1699

CHARLOTTE
W4)TER
August 30, 2024
ATTN: Mr. Joe Corporon
Division of Water Resources
Environmental Specialist 1
Water Quality Permitting Section — NPDES
1617 Mail Service Center
Raleigh NC 27699-1617
RECEIVED
SE;' li 4 r:3Z4
NCDEQ/D` qR/NPDES
Subject: NPDES Permit Renewal Application
Lee S. Dukes Water Treatment Plant - NPDES Permit # NCO084387
Mr. Corporon,
Charlotte Water would like to request renewal of NPDES permit NCO084387 at the Lee S. Dukes
Water Treatment Plant. The current permit is set to expire February 28, 2025. Lee S. Duke
Water Treatment Plant is categorized as a conventional technology water treatment plant
[utilizing coagulation, flocculation, sedimentation, and filtration] with discharge of wastewaters
from media filter backwash and sedimentation basins. For your review, and in lieu of the Short
Form 2C we have provided in the past for permit renewals, I am providing the full EPA Form 2 C.
Please find attached to Form C the following:
• Appendix A: Outfall Location Map
• Appendix B: Line Drawing / Treatment Process Synopsis
• Appendix C: Waiver Request
• Appendix D: Biological Toxicity Test
• Appendix E : Additional Information
There are a couple of elements that I want to bring to your awareness and/or consideration:
• Relative to Section 6 -Improvements of the permit application, no construction or
improvements have been made to the wastewater treatment process at Dukes since the
last permit was implemented.
• As a point of reference, all water quality results from the last 3.5 years are included
within Section 7.
• Given the excellence of the effluent water quality discharged from the facility as
validated by the empirical data required by the current NPDES permit, Charlotte Water
Charlotte Water 5200 Brookshire Blvd, Charlotte, NC 28216 charlottewater.org
operated by the City of Charlotte

does not see the justification in being required to add the following Table A pollutants
to our monitoring requirements: COD, BOD and TOC.
• Since ferrous sulfate is not currently being utilized in the treatment process, we
respectfully ask that quarterly Total Iron monitoring be removed from the permit
sampling requirements.
If you have any questions, please contact Dave Banick at 704-634-9104 or Gabe Sasser at 704-
507-5267.
Sincerely,
C4
Gabe Sasser
Operations Manager
City of Charlotte
Charlotte Water
Charlotte Water 5200 Brookshire Blvd, Charlotte, NC 28216 charlottewater.org
lb Operated by the City of Charlotte

United States Office of Water EPA Form 3510-2C
Environmental Protection Agency Washington, D.C. Revised March 2019
Water Permits Division
� E�► Application Form 2C
Existing Manufacturing,
Commercial, Mining, and
Si Ivicu Itu ral Operations
NPDES Permitting Program
Note: Complete this form and Form 1 if your facility is an existing manufacturing, commercial, mining,
or silvicultural facility that currently discharges process wastewater.

Paperwork Reduction Act Notice
The U.S. Environmental Protection Agency estimates the average burden to collect information and
complete Form 2C to be 32.5 hours. This estimate includes time for reviewing instructions, searching
existing data sources, gathering and maintaining the needed data, and completing and reviewing the
collection of information. Send comments about the burden estimate or any other aspect of this
collection of information to the Chief, Information Policy Branch (PM-223), U.S. Environmental
Protection Agency, 1200 Pennsylvania Avenue, NW, Washington, DC 20460, and to the Office of
Information and Regulatory Affairs, Office of Management and Budget, 725 17t' Street, NW,
Washington, DC 20503, marked "Attention: Desk Officer for EPA."

FORM 2C—INSTRUCTIONS
General Instructions
Who Must Complete Form 2C?
You must complete Form 2C if you answered "Yes" to Item 1.2.2 on
Form 1—that is, if you are an existing manufacturing, commercial,
mining, or silvicultural facility that currently discharges process
wastewater.
Where to File Your Completed Form
Submit your completed application package (Forms 1 and 2C) to
your National Pollutant Discharge Elimination System (NPDES)
permitting authority. Consult Exhibit 1-1 of Form 1's "General
Instructions" to identify your NPDES permitting authority.
Public Availability of Submitted Information
The U.S. Environmental Protection Agency (EPA) will make
information from NPDES permit application forms available to the
public for inspection and copying upon request. You may not claim
any information on Form 2C (or related attachments) as
confidential.
You may make a claim of confidentiality for any information that you
submit to EPA that goes beyond the information required by Form
2C. Note that NPDES authorities will deny claims for treating any
effluent data as confidential. If you do not assert a claim of
confidentiality at the time you submit your information to the
NPDES permitting authority, EPA may make the information
available to the public without further notice to you. EPA will handle
claims of confidentiality in accordance with the Agency's business
confidentiality regulations at Part 2 of Title 40 of the Code of
Federal Regulations (CFR).
Completion of Forms
Print or type in the specified areas only. If you do not have enough
space on the form to answer a question, you may continue on
additional sheets, as necessary, using a format consistent with the
form.
Provide your EPA Identification Number from the Federal Registry
Service, NPDES permit number, and facility name at the top of
each page of Form 2C and any attachments. If you do not know
your EPA Identification Number, contact your NPDES permitting
authority. See Exhibit 1-1 of Form Ts "General Instructions" for
contact information. Additionally, for Tables A through E, provide
the applicable outfall number at the top of each page.
Do not leave any response areas blank unless the form directs you
to skip them. If the form directs you to respond to an item that does
not apply to your facility or activity, enter "NA" for "not applicable" to
show that you considered the item and determined a response was
not necessary for your facility.
The NPDES permitting authority will consider your application
complete when it and any supplementary material are received and
completed according to the authority's satisfaction. The NPDES
permitting authority will judge the completeness of any application
independently of the status of any other permit application or permit
for the same facility or activity.
Definitions
The legal definitions of all key terms used in these instructions and
Form 2C are in the "Glossary" at the end of the "General
Instructions" in Form 1.
Line -by -Line Instructions
Section 1.Outfall Location
Item 1.1. Identify each of the facility's outfall structures by number.
For each outfall, specify the latitude and longitude to the nearest
15 seconds and name of the receiving water. The application form
provides reporting space for three outfalls. If your facility has more
than this number, attach additional sheets as necessary. The
location of each outfall (i.e., where the coordinates are collected)
shall be the point where the discharge is released into a water of
the United States. Latitude and longitude coordinates may be
obtained in a variety of ways, including use of hand held devices
(e.g., a GPS enabled smartphone), internet mapping tools (e.g.,
httos://mynasadata.larc.nasa.gov/latitudelonciitude-finder/ ,
geographic information systems (e.g., ArcView), or paper maps
from trusted sources (e.g., U.S. Geological Survey or USGS).For
further guidance, refer to
hUp://www.epa.gov/geospatial/latitudelongitude-data-standard.
Section 2. Line Drawing
Item 2.1. Attach a line drawing showing water flow through your
facility, from intake to discharge. Indicate the sources of intake
water (e.g., city, well, stream, other); operations contributing
wastewater to the effluent including process and production areas,
sanitary flows, cooling water, and stormwater runoff; and treatment
units labeled to correspond to the more detailed descriptions under
Section 3. You may group similar operations into a single unit.
Construct a water balance on the line drawing by showing average
flows (specify units) between intakes, operations, treatment units,
and outfalls. Show all significant losses of water to products, the
atmosphere, and discharge. You should use actual measurements
wherever available; otherwise use your best estimate. If you cannot
determine a water balance for your activities (such as mining
activities), provide a pictorial description of the nature and amount
of any sources of water and any collection and treatment measures.
An example of an acceptable line drawing is provided in Exhibit
2C-1 at the end of these instructions.
Section 3. Average Flows and Treatment
Item 3.1. For each outfall identified under Item 1.1, provide the
following information: (1) all processes, operations, or production
areas that contribute wastewater to the effluent for the outfall,
including process wastewater, sanitary wastewater, cooling water,
and stormwater runoff; (2) average flow of wastewater contributed
by each operation in million gallons per day (mgd); (3) a description
of the treatment unit (including size of each treatment unit, flow rate
through each treatment unit, retention time, etc.); (4) the applicable
treatment code(s) from Exhibit 2C-2 (see end of instructions); and
(5) the ultimate disposal of any solid or fluid wastes that are not
discharged to the receiving water. You may describe processes,
operations, or production areas in general terms (e.g., "dye -making
reactor" or "distillation tower"). You may estimate the average flow
of point sources composed of stormwater; however, you must
2C-1

FORM 2C—INSTRUCTIONS CONTINUED
indicate the basis of the rainfall event and the method of estimation.
Add additional sheets as necessary.
Item 3.2. Answer whether you are applying for an NPDES permit to
operate a privately owned treatment works. If yes, continue to Item
3.3. If no, skip to Section 4.
Item 3.3. Attach a list to your application that includes the identity of
each user of the treatment works, then answer "Yes" to Item 3.3.
Section 4. Intermittent Flows
Item 4.1. Answer "Yes" or "No" to indicate whether any of the
discharges you described in Sections 1 and 3 of Form 2C are
intermittent or seasonal, except for stormwater runoff, spillage, or
leaks. An intermittent discharge is one that is not continuous. A
continuous discharge is one that occurs without interruption during
the operating hours of the facility, except for infrequent shutdowns
for maintenance, process changes, or other similar activities. A
discharge is seasonal if it occurs only during certain parts of the
year. If yes, continue to Item 4.2. If no, skip to Section 5.
Item 4.2. By relevant outfall number, identify each operation that
has intermittent or seasonal discharges. Indicate the average
frequency (days per week and months per year), the long-term
average and maximum daily flow rates in mgd, and the duration of
the intermittent or seasonal discharges. Base your answers on
actual data if available. Otherwise, provide your best estimate.
Report the average of all daily values measured during days when
the discharge occurred for "Long -Term Average," and report the
highest daily value for "Maximum Daily."
Section 5. Production
Item 5.1. Indicate whether any effluent limitation guidelines (ELGs)
promulgated under Section 304 of the Clean Water Act (CWA)
apply to your facility. If yes, continue to Item 5.2. If no, skip to
Section 6. All ELGs promulgated by EPA appear in the Federal
Register and are published annually in 40 CFR Subchapter N. An
ELG applies if you have any operations contributing process
wastewater in any subcategory covered by a Best Practicable
Control Technology Currently Available (BPT), Best Conventional
Pollutant Control Technology (BCT), or Best Available Technology
Economically Achievable (BAT) guideline. If you are unsure
whether you are covered by a promulgated ELG, consult your
NPDES permitting authority (see Exhibit 1-1 of the "General
Instructions" of Form 1). You must check "Yes" if an applicable ELG
has been promulgated, even if the ELG is being contested in court.
If you believe that a promulgated ELG has been remanded for
reconsideration by a court and does not apply to your operations,
you may answer "No" to Item 5.1 and skip to Section 6.
Item 5.2. Complete Item 5.2 by indicating the applicable ELG
category, ELG subcategory, and corresponding regulatory citation.
See the example below.
J
5.2
ELG Category
ELG
Subcategory
Regulatory
Citation
Pulp, Paper,
Secondary Fiber
40 CFR 430,
and
Non-Deink
Subpart J
Paperboard
Subcategory
a
Point Source
a
Category
Item 5.3. Indicate if the limitations in the applicable ELGs are
expressed in terms of production or other measure of operation. For
operational parameter, it is expressed in terms of production (e.g.,
"pounds of biological oxygen demand per cubic foot of logs from
which bark is removed," or "pounds of total suspended solids per
megawatt hour of electrical energy consumed by smelting
furnace"). An example of an ELG not expressed in terms of a
measure of operation is one that limits the concentration of
pollutants. If yes, continue to Item 5.4. If no, skip to Section 6.
Item 5.4. Indicate the operations, products, or materials produced
at the facility for each outfall. For each operation, product, or
material produced, denote the quantity produced per day using the
measurement units specified in the applicable ELG. The NPDES
permitting authority will use the production information to apply
ELGs to your facility. You may not claim that the production
information you submit is confidential. You do not need to indicate
how you calculated the reported information. The production figures
provided must be based on a reasonable measure of actual daily
production, not on design capacity or on predictions of future
operations. To obtain alternate limits under 40 CFR 122.45(b)(2)(ii),
you must define your maximum production capability and
demonstrate to the NPDES permitting authority that your actual
production is substantially below maximum production capability
and that there is a reasonable potential for an increase above
actual production during the duration of the permit.
Section 6. Improvements
Item 6.1. Indicate if you are required by any federal, state, or local
authority to meet an implementation schedule for constructing,
upgrading, or operating wastewater treatment equipment or
practices or any other environmental programs that could affect the
discharges described in your application. The requirements include,
but are not limited to, permit conditions, administrative enforcement
orders, enforcement compliance schedule letters, stipulations, court
orders, and grant or loan conditions. If yes, continue to Item 6.2. If
no, skip to Item 6.3.
Item 6.2. Briefly identify and describe each applicable project (e.g.,
consent decree, enforcement order, or permit condition). For each
condition, specify the affected outfall number(s), the source(s) of
the discharge, the projected final compliance date, and the
required final compliance date.
Item 6.3.OPTIONAL ITEM. If desired, attach descriptions of any
additional water pollution control programs (or other environmental
projects that could affect your discharges) that are now underway
or planned. Indicate in your attachments whether each program is
actually underway or is planned, and indicate your actual or
planned schedule for construction.
Section 7. Effluent and Intake Characteristics
Items 7.1 to 7.17. These items require you to collect and report
data for the parameters and pollutants listed in Tables A through E,
located at the end of Form 2C. The instructions for completing the
tables are table -specific in addition to the criteria for determining
who should complete them. In general, the following conditions
apply:
2C-2

FORM 2C—INSTRUCTIONS CONTINUED
Table
Pollutants/Parameters
Who Completes?
A
Conventional and non-
All applicants from all outfalls unless a
conventional pollutants
waiver is obtained from the NPDES
permitting authority.
B
Toxic metals, cyanide, total
Applicants in the primary industry
phenols, and organic toxic
categories listed in Exhibit 2C-3 at the
pollutants
end of these instructions.
C
Certain conventional and
Applicants subject to ELGs that limit
non -conventional pollutants
pollutants directly or indirectly and
applicants who believe pollutants may
be present in their facility's discharge.
D
Certain hazardous
Applicants who believe pollutants may
substances and asbestos
be present in their fact li 's dischar e.
E
2,3,7,8-tetrachlorodibenzo-
Applicants that use or manufacture the
p-dioxin (2,3,7,8-TCDD)
pollutant or believe the pollutant may
be present in the facility's discharge.
Important note: Read the "General Instructions for Reporting,
Sampling, and Analysis" on pages 2C-5 and 2C-6 before
completing Section 7 and Tables A through E.
Item 7.1 and Table A. All applicants must report at least one
analysis for each conventional and non -conventional pollutant listed
in Table A for each outfall (one table per outfall). This includes
outfalls discharging only noncontact cooling water or stormwater
runoff. However, at your request, the NPDES permitting authority
may waive the requirement to test for one or more of the listed
pollutants for specific outfalls, upon a determination that available
information is adequate to support issuance of your NPDES permit
with less stringent reporting requirements. You may also request a
waiver from your NPDES permitting authority for one or more of the
Table A pollutants for your industry category or subcategory.
Indicate whether you are requesting a waiver in response to Item
7.1. If yes, continue to Item 7.2. If no, skip to Item 7.3.
Item 7.2. Specify the outfalls for which you are requesting a waiver.
Next, indicate on Table A for the applicable outfalls the pollutants
for which the waiver is being requested. Attach your waiver request
and supporting information to your completed Form 2C.
Item 7.3. Test your effluent from each outfall for each pollutant
listed in Table A for which you have not requested a waiver. You
may also conduct optional tests of your intake water for the Table A
pollutants. See the "General Instructions for Reporting, Sampling,
and Analysis" on pages 2C-5 and 2C-6 for further information.
Item 7.4 and Table B. This item asks whether any of the facility's
processes that contribute wastewater fall into one or more of the
primary industry categories listed in Exhibit 2C-3. If you are
applying for a permit for a privately owned treatment works,
determine your testing requirements based on the industrial
categories of your contributors. This exercise is simply to determine
your testing requirements only. You are not giving up your right to
challenge your inclusion in the category determined for testing (e.g.,
for deciding whether an ELG is applicable) before your permit is
issued. If yes, continue to Item 7.5. If no, skip to Item 7.8.
Complete a separate Table B for each outfall. Section 1 of Table B
lists toxic metals, cyanide, and total phenols. Sections 2 through 5
of Table B list the pollutants in each of the gas
chromatography/mass spectrometry (GC/MS) fractions. Note that
inclusion of total phenols in Section 1 of Table B does not mean
that EPA is classifying the group as toxic pollutants.
Item 7.5. Because you indicated in Item 7.4 that the facility's
processes contribute wastewater that falls into one or more of the
primary industry categories, check "Testing Required" for all toxic
metals, cyanide, and total phenols in Section 1 of Table B. Answer
"Yes" to Item 7.5 once you have completed this task.
Item 7.6. Because you indicated in Item 7.4 that the facility's
processes contribute wastewater that falls into one or more of the
primary industry categories, list the primary industry categories
applicable to your facility. Next, review Exhibit 2C-3 to determine
whether testing is required and for which GC/MS fraction(s): volatile
compounds, acid compounds, base/neutral compounds, and
pesticides. Check the applicable boxes for each GC/MS fraction
requiring testing.
Item 7.7. For each of the required GC/MS fractions, check "Testing
Required" for each of the pollutants in the required fraction in
Sections 2 through 5 of Table B. Answer "Yes" to Item 7.7 once you
have completed this task.
Item 7.8 and Sections 1 through 5 of Table B. For all other cases
(secondary industries, nonprocess wastewater outfalls, and
nonrequired GC/MS fractions) and remaining pollutants, check
"Believed Present" or "Believed Absent" in Sections 1 through 5 of
Table B to indicate whether you have reason to believe that any of
the pollutants listed are discharged from your outfalls. Answer "Yes"
to Item 7.8 after you have completed this step.
Item 7.9 and Section 1 of Table B. For each pollutant you know or
have reason to believe is present in your discharge from each
applicable outfall in concentrations of 10 parts per billion (ppb) or
greater, you must report quantitative data. For every pollutant
expected to be discharged in concentrations less than 10 ppb, you
must submit quantitative data or briefly describe the reasons the
pollutant is expected to be discharged. For pollutants in intake
water, see the discussion under "General Instructions for Reporting,
Sampling, and Analysis" below. Answer "Yes" to Item 7.9 once you
have completed Section 1 of Table B.
Item 7.10. This item asks if you qualify as a "small business." If so,
you are exempt from submitting quantitative data for the organic
toxic pollutants on Table B (Sections 2 through 5). You still must
indicate, though, whether you believe any of the pollutants listed in
Sections 1 through 5 are present in your discharge per the
Instructions at Item 7.8 above.
You can qualify as a small business in two ways: (1) If your facility
is a coal mine and if your probable total annual production is less
than 100,000 tons per year, you may submit past production data
or estimated future production (such as a schedule of estimated
total production under 30 CFR 795.14(c)) instead of conducting
analyses for the organic toxic pollutants. (2) If your facility is not a
coal mine and if your gross total annual sales for the most recent
three years average less than $100,000 per year (in second
quarter 1980 dollars), you may submit sales data for those years
instead of conducting analyses for the organic toxic pollutants.
The production or sales data must be for the facility that is the
source of the discharge. The data should not be limited to
production or sales for the process or processes that contribute to
the discharge, unless those are the only processes at your facility.
2C-3

FORM 2C—INSTRUCTIONS CONTINUED
For sales data, in situations involving intra-corporate transfer of
goods and services, the transfer price per unit should approximate
market prices for those goods and services as closely as possible.
Sales figures for years after 1980 should be indexed to the second
quarter of 1980 by using the gross national product price deflator
(second quarter of 1980 = 100). This index is available online from
the U.S. Department of Commerce, Bureau of Economic Analysis
at hftp://bea.gov/national/pdf/SNTables.pdf.
If you qualify as a small business according to the criteria above,
answer "Yes" to Item 7.10. Check the box at the top of Table B to
show that you are not required to submit quantitative data for the
organic toxic pollutants (Sections 2 through 5 of Table B), then
skip to Item 7.12. Otherwise, answer "No" and continue to Item
7.11.
Item 7.11 and Sections 2 through 5 of Table B. Unless you
qualify as a small business (see Item 7.10), you must provide
quantitative data for all pollutants for which you marked "Testing
Required" in Sections 2 through 5 of Table B. You must also
provide quantitative data for all pollutants you marked as "Believed
Present" in Sections 2 through 5 of Table B if you discharge those
pollutants in concentrations of 10 ppb or greater, except for
acrolein, acrylonitrile, 2,4-dinitrophenol, and 2-methyl-4,6-
dinitrophenol. If you discharge any of the four latter pollutants in
concentrations of 100 ppb or greater, you must report quantitative
data. If you discharge the pollutants in Sections 2 through 5 of
Table B less than these thresholds (i.e., <100 ppb for acrolein,
acrylonitrile, 2,4-dinitrophenol, and 2-methyl-4,6-dinitrophenol and
<10 ppb for all others), you must submit quantitative data or briefly
describe the reasons the pollutant is in your discharge.
For pollutants in intake water, see the discussion under "General
Instructions for Reporting, Sampling, and Analysis" on pages
2C-5 and 2C-6 for further information.
Once you have completed these tasks, answer "Yes" to Item 7.11.
Item 7.12 and Table C. For each outfall (including outfalls
containing only noncontact cooling water or stormwater runoff),
indicate whether you know or have reason to believe that any of
the pollutants listed on Table C are present in your discharge. If
so, mark the box in the "Believed Present" column for each
applicable pollutant. If not, mark the box in the "Believed Absent"
column for each applicable pollutant. Answer "Yes" to Item 7.12
once you have completed the required task for each outfall.
Item 7.13 and Table C. You are required to report quantitative
data for any Table C pollutants that are directly limited in an
applicable ELG or are indirectly limited in an applicable ELG
through an expressed limitation on an indicator (e.g., use of total
suspended solids (TSS) as an indicator to control the discharge
of iron and aluminum). For all other pollutants that you marked as
"Believed Present," you must either report quantitative data or
briefly describe the reasons the pollutant is expected to be
discharged.
For pollutants in intake water, see the discussion under "General
Instructions for Reporting, Sampling, and Analysis" on pages
2C-5 and 2C-6 for further information.
Answer "Yes" to Item 7.13 when you have fully completed the
tasks associated with Table C and Items 7.12 and 7.13 above.
Item 7.14 and Table D. For each outfall, indicate if you believe that
any pollutant listed in Table D is "Believed Present" or "Believed
Absent" in your facility's effluent. Check the boxes in the applicable
columns on Table D next to each pollutant. For every pollutant
believed present, you must briefly describe the reasons the
pollutant is expected to be discharged and report any quantitative
data you have for that pollutant. Note that you are not required to
perform analytical tests for any of the Table D pollutants at this time.
However, if you have prior test results, you must report them.
Item 7.15. Answer "Yes" to this Item when you have completed
Table D.
Under 40 CFR 117.12(a)(2), certain discharges of hazardous substances
(listed in Exhibit 2C4 at the end of these instructions) may be exempted from
the requirements of Section 311 of the CWA, which establishes reporting
requirements, civil penalties, and liability for cleanup costs for spills of oil and
hazardous substances. A discharge of a particular substance can be exempted
if the origin, source, and amount of the discharged substances are identified in
the NPDES permit application or in the permit, if the permit contains a
requirement for treatment of the discharge, and if the treatment is in place.
Exemptions are allowed from the requirements of CWA Section 311.
Applications for exemptions must set forth the following information:
1. The substance and the amount of each substance that may be discharged.
2. The origin and source of the discharge of the substance.
3. The treatment to be provided for the discharge by:
a. An onsite treatment system separate from any treatment system
treating your normal discharge;
b. A treatment system designed to treat your normal discharge and that is
additionally capable of treating the amount of the substance identified
under paragraph 1 above; or
c. Any combination of the above.
See 40 CFR 117.12(a)(2) and (c) or contact your NPDES permitting authority
for further information on exclusions from CWA Section 311.
Item 7.16. Indicate whether:
• Your facility uses or manufactures 2,4,5-trichlorophenoxy
acetic acid (2,4,5-T); 2-(2,4,5-trichlorophenoxy) propanoic acid
(Silvex, 2,4,5-TP); 2-(2,4,5-trichlorophenoxy) ethyl 2,2-dichloro-
propionate (Erbon); 0,0-dimethyl 0-(2,4,5-trichlorophenyl)
phosphorothioate (Ronnel); 2,4,5,-trichlorophenol (TCP); or
hexachlorophene (HCP).
• You know or have reason to believe that 2.3.7,8-
tetrachlorodibenzo-p-dioxin (TCDD) is or may be present in an
effluent.
If yes, continue to Item 7.17. If no, skip to Section 8.
Item 7.17 and Table E. If you answered "Yes" to Item 7.16, you
must report qualitative data, generated using a screening procedure
not calibrated with analytical standards, for TCDD. Your screening
analyses must be performed using gas chromatography with an
electron capture detector. A TCDD standard for quantitation is not
required. Describe the results of your screening analysis (e.g., "no
measurable baseline deflection at the retention time of TCDD" or "a
measurable peak within the tolerances of the retention time of
TCDD.") on Table E. The NPDES permitting authority may require
you to perform a quantitative analysis if you report a positive result.
Answer "Yes" to Item 7.17 when you have completed Table E
2C-4

General Instructions for Reporting, Sampling, and Analysis
Important note: Read these instructions before completing Tables All reporting of values for metals must be in terms of "total
A through E and Section 7 of Form 2C. recoverable metal," unless:
General Items
Complete the applicable tables for each outfall at your facility. Be
sure to note the EPA Identification Number, NPDES permit number,
facility name, and applicable outfall number at the top of each page
of the tables and any associated attachments.
You may report some or all of the required data by attaching
separate sheets of paper instead of completing Tables A through E
for each of your outfalls so long as the sheets contain all of the
required information and are similar in format to Tables A through
E. For example, you may be able to print a report in a compatible
format from the data system used in your GC/MS analysis
completed under Table B.
Table A requires you to report at least one analysis for each
pollutant listed. Tables B through D require you to report analytical
data in two ways. For some pollutants, you may be required to
check the box in the "Testing Required" column and test and report
the levels of the pollutants in your discharge whether or not you
expect them to be present in your discharge. For all other
pollutants, you must check the box in either the "Believed Present'
or "Believed Absent' columns based on your best estimate and test
for those you believe to be present (with some exceptions). Base
your determination that a pollutant is present in or absent from your
discharge on your knowledge of your raw materials, maintenance
chemicals, intermediate and final products and byproducts, and any
previous analyses known to you of your effluent or similar effluent.
For example, if you manufacture pesticides, you should expect
those pesticides to be present in contaminated stormwater runoff.
If you would expect a pollutant to be present solely because of its
presence in your intake water, you must mark "Believed Present'
but you are not required to analyze for that pollutant. Instead, mark
an "X" in the long-term average value of the "Intake" column;
optionally, you may instead provide intake data.
Reporting of Effluent Data
Report sampling results for all pollutants in Tables A through C as
concentration and total mass, except for flow, temperature, pH,
color, and fecal coliform organisms. If you are reporting
quantitative data under Table D, report concentration only.
Flow, temperature, pH, color, and fecal coliform organisms must be
reported as mgd, degrees Celsius (°C), standard units, color units,
and most probable number per 100 milliliters (MPN/100 mL),
respectively. Use the following abbreviations in the columns
requiring "units" in Tables A through D.
Concentration
Mass
ppm = parts per million
Ibs = pounds
mg/L = milligrams per liter
ton = tons (English tons)
ppb = parts per billion
mg = milligrams
pg/L = micrograms per liter
g = grams
MPN = most probable number per
kg = kilograms
100 milliliters
T = tonnes (metric tons)
• An applicable, promulgated ELG specifies the limitation for
the metal in dissolved, valent, or total form;
All approved analytical methods for the metal inherently
measure only its dissolved form (e.g., hexavalent chromium);
or
• The permitting authority has determined that in establishing
case -by -case limitations it is necessary to express the
limitations of the metal in dissolved, valent, or total form to
carry out the provisions of the CWA.
Note that you are not required to complete the "Maximum Monthly
Discharge" and the "Long -Term Average Daily Discharge" columns
of Tables A through C; however, these fields should be completed
if data are available.
If you measure only one daily value, complete the "Maximum Daily
Discharge" columns of the tables and enter " 1" in the "Number of
Analyses" columns. The NPDES permitting authority may require
additional analyses to further characterize your discharges.
For composite samples, the daily value is the total mass or
average concentration found in a composite sample taken over the
operating hours of the facility during a 24-hour period. For grab
samples, the daily value is the arithmetic or flow -weighted total
mass or average concentration found in a series of at least four
grab samples taken over the operating hours of the facility during a
24-hour period.
If you measure more than one daily value for a pollutant and those
values are representative of your wastestream, you must report
them. You must describe your method of testing and data analysis.
When an applicant has two or more outfalls with substantially
identical effluents, the NPDES permitting authority may allow the
applicant to test only one outfall and report those quantitative data
as applying to the substantially identical outfall. If the permitting
authority grants your request, attach a separate sheet to the
application form identifying the ouff all tested and describing why the
other outfall(s) are substantially identical.
Reporting of Intake Data
You are not required to report data under the `Intake" columns of
Tables A through C unless you wish to demonstrate your eligibility
for a "net' effluent limitation for one or more pollutants in Tables A
through C (i.e., an effluent limitation adjusted by subtracting the
average level of the pollutant(s) present in your intake water).
NPDES regulations allow net limitations only in certain
circumstances. To demonstrate your eligibility, under the `Intake"
columns report the average of the results of analyses of your intake
water and discuss the requirements for a net limitation with your
NPDES permitting authority. If your water is treated before use, test
the water after it has been treated.
2C-5

General Instructions for Reporting, Sampling, and Analysis Continued
Sampling
The collection of samples for the reported analyses should be
supervised by a person experienced in performing sampling of
industrial wastewater. You may contact your NPDES permitting
authority for detailed guidance on sampling techniques and for
answers to specific questions. See Exhibit 1-1 of Form 1 for
contact information. Any specific requirements in the applicable
analytical methods —for example, sample containers, sample
preservation, holding times, and the collection of duplicate
samples —must be followed.
The time when you sample should be representative of your normal
operation, to the extent feasible, with all processes that contribute
wastewater in normal operation, and with your treatment system
operating properly with no system upsets. Collect samples from the
center of the flow channel, where turbulence is at a maximum, at a
site specified in your present NPDES permit, or at any site
adequate for the collection of a representative sample.
Grab samples must be used for pH, temperature, cyanide, total
phenols, residual chlorine, oil and grease, fecal coliform (including
E. coli), and enterococci (previously known as fecal streptococcus
at 40 CFR 122.26(d)(2)(iii)(A)(3)), and volatile organic compounds.
For all other pollutants, a 24-hour composite sample, using a
minimum of four grab samples, must be used unless specified
otherwise at 40 CFR 136. However, a minimum of one grab
sample may be taken for effluents from holding ponds or other
impoundments with a retention period greater than 24 hours.
For stormwater discharges, a minimum of one to four grab
samples must be taken, depending on the duration of the
discharge. One grab sample must be taken in the first hour (or
less) of discharge, with one more grab sample (up to a minimum of
four) taken in each succeeding hour of discharge for discharges
lasting four hours or more.
Except for stormwater discharges, the NPDES permitting authority
may waive composite sampling requirements for any outfall for
which you demonstrate that use of an automatic sampler is
infeasible and that the minimum of four grab samples will be
representative of your discharge. Results of analyses of individual
grab samples for any parameter may be averaged to obtain the
daily average. Grab samples that are not required to be analyzed
immediately may be composited in the laboratory, if the container,
preservation, and holding time requirements are met and if sample
integrity is not compromised during compositing. See Table II at 40
CFR 136.3 for further information.
A grab sample is an individual sample of at least 100 milliliters
collected at a randomly chosen time over a period not exceeding
15 minutes.
A composite sample is a combination of at least eight sample
aliquots of at least 100 milliliters, collected at periodic intervals
during the operating hours of a facility over a 24-hour period. The
composite must be flow proportional; either the time interval
between each aliquot or the volume of each aliquot must be
proportional to either the stream flow at the time of sampling or the
total stream flow since the collection of the previous aliquot.
Aliquots may be collected manually or automatically. For "GC/MS
Fraction —Volatile Compounds" in Table B, aliquots must be
combined in the laboratory immediately before analysis. Four
(rather than eight) aliquots or grab samples should be collected for
this fraction. These four samples should be collected during actual
hours of discharge over a 24-hour period and need not be flow
proportioned. Only one analysis is required.
Use of Historical Data
Existing data may be used, if available, in lieu of sampling
conducted solely for the purposes of this application, provided that:
all data requirements are met; sampling was performed, collected,
and analyzed no more than 4.5 years prior to submission; all data
are representative of the discharge; and all available representative
data are considered in the values reported.
Analysis
Except as specified below, all required quantitative data shall be
collected in accordance with sufficiently sensitive analytical
methods approved under 40 CFR 136 or required under 40 CFR
chapter I, subchapter N or 0. A method is "sufficiently sensitive"
when:
• The method minimum level (ML) is at or below the level of the
applicable water quality criterion for the measured pollutant or
pollutant parameter.
• The method ML is above the water quality criterion, but the
amount of the pollutant or pollutant parameter in the facility's
discharge is high enough that the method detects and
quantifies the level of the pollutant or pollutant parameter in
the discharge.
• The method has the lowest ML of the analytical methods
approved under 40 CFR 136 or required under 40 CFR
chapter I, subchapter N or 0 for the measured pollutant or
pollutant parameter.
Consistent with 40 CFR 136, you may provide matrix- or sample -
specific MLs rather than the published levels. Further, where you
can demonstrate that, despite a good faith effort to use a method
that would otherwise meet the definition of "sufficiently sensitive,"
the analytical results are not consistent with the quality assurance
(QA)/quality control (QC) specifications for that method, then the
NPDES permitting authority may determine that the method is not
performing adequately and the NPDES permitting authority should
select a different method from the remaining EPA -approved
methods that is sufficiently sensitive consistent with 40 CFR
122.21(e)(3)(i). Where no other EPA -approved methods exist, you
must select a method consistent with 40 CFR 122.21(e)(3)(ii).
When there is no analytical method that has been approved under
40 CFR 136; required under 40 CFR chapter I, subchapter N or 0,
and is not otherwise required by the NPDES permitting authority,
you may use any suitable method but shall provide a description of
the method. When selecting a suitable method, other factors such
as a method's precision, accuracy, or resolution, may be
considered when assessing the performance of the method.
2C-6

FORM 2C—INSTRUCTIONS CONTINUED
Section 8. Used or Manufactured Toxics
Item 8.1. Indicate if any pollutant listed in Table B is used or
manufactured in your facility as an intermediate or final product or
byproduct. If yes, continue to Item 8.2. If no, skip to Section 9.
Item 8.2. List the applicable toxic pollutants. Note that the NPDES
permitting authority may waive or modify the requirement if you
demonstrate that it would be unduly burdensome to identify each
toxic pollutant and the permitting authority has adequate
information to issue you a permit. You may not claim this
information as confidential. Note that you do not need to distinguish
between use or production of the pollutants or list amounts.
Section 9. Biological Toxicity Tests
Item 9.1. Indicate if you have any knowledge or reason to believe
that any biological test for acute or chronic toxicity has been made
on any of your discharges or on a receiving water in relation to your
discharge within the last three years. If yes, continue to Item 9.2. If
no, skip to Section 10.
Item 9.2. Identify the tests known to have been performed and the
purposes of each. For each test, check "Yes" or "No" to indicate if
you have submitted the test results to the NPDES permitting
authority and the date the results were submitted. The NPDES
permitting authority may ask you to provide additional details after
reviewing your application.
Section 10. Contract Analyses
Item 10.1. Indicate if any of the analyses reported in Section 7 were
performed by a contract laboratory or consulting firm. If yes,
continue to Item 10.2. If no, skip to Section 11.
Item 10.2. Identify each laboratory or firm used in the table
provided. For each, provide the name, address, and phone number
of the laboratory or firm and the pollutants analyzed.
Section 11. Additional Information
Item 11.1. In addition to the information reported on the application
form, the NPDES permitting authority may request additional
information reasonably required to assess the discharges of the
facility and to determine whether to issue an NPDES permit. The
additional information may include additional quantitative data and
bioassays to assess the relative toxicity of discharges to aquatic life
and requirements to determine the cause of the toxicity. Indicate
under Item 11.1 whether the NPDES permitting authority has
requested additional information from you. If yes, continue to Item
11.2. If no, skip to Section 12.
Item 11.2. List the items requested and attach the required
information to the application.
Section 12. Checklist and Certification Statement
Item 12.1. Review the checklist provided. In Column 1, mark the
sections of Form 2C that you have completed and are submitting
with your application. In Column 2, indicate for each section
whether you are submitting attachments.
Item 12.2. The CWA provides for severe penalties for submitting
false information on this application form. Section 309(c)(2) of the
CWA provides that "Any person who knowingly makes any false
statement, representation, or certification in any application, ...shall
upon conviction, be punished by a fine of no more than $10,000 or
by imprisonment for not more than six months or both."
FEDERAL REGULATIONS AT 40 CFR 122.22 REQUIRE THIS
APPLICATION TO BE SIGNED AS FOLLOWS:
A. For a corporation, by a responsible corporate officer. For the
purpose of this section, a responsible corporate officer means:
(1) a president, secretary, treasurer, or vice-president of the
corporation in charge of a principal business function, or any
other person who performs similar policy- or decision -making
functions for the corporation, or (2) the manager of one or
more manufacturing, production, or operating facilities,
provided the manager is authorized to make management
decisions which govern the operation of the regulated facility
including having the explicit or implicit duty of making major
capital investment recommendations, and initiating and
directing other comprehensive measures to assure long term
environmental compliance with environmental laws and
regulations; the manager can ensure that the necessary
systems are established or actions taken to gather complete
and accurate information for permit application requirements;
and where authority to sign documents has been assigned or
delegated to the manager in accordance with corporate
procedures.
B. For a partnership or sole proprietorship, by a general partner
or the proprietor, respectively.
C. For a municipality, state, federal, or other public facility, by
either a principal executive officer or ranking elected official.
For purposes of this section, a principal executive officer of a
federal agency includes: (1) The chief executive officer of the
agency, or (2) a senior executive officer having responsibility
for the overall operations of a principal geographic unit of the
agency (e.g., Regional Administrators of EPA).
END
2C-7
Submit your completed Form 1, Form 2C, and
all associated attachments
(and any other required NPDES application forms)
to your NPDES permitting authority.

Exhibit 2CA Example Line Drawing
Blue River
Municipal
90,000 GPD Water Supply Blue River
10,000 GPD
45,000 GPDt
30,000 GPD
Cooling Water
Raw Materials
Fiber
15 0,000
Washing
10 000
Ding
5,000 GPD
10,000 GPD
Preparation
GPD
PD
GPD
To Atmospf
40,000 GPD
40,000 10,000
To Product
GPD GPD
5,000 GPD
Solid Waste
Grit
sWasteSeparator0"
4,000 GPD
Treatment
Plant #2
36,000 GPD
34,000 GPD
j Outfa11002
50,000 GPD
Waste
Outfall001
Stormwater
Treatment
70,000 GPD + Storrnwa r
Max- 20 000 GPD
Plant #1
Schematic of Water Flow
Brown Mills, Inc.
City, County, State
2C-8

Exhibit 2C-2. Codes for Treatment Units and Disposal of Wastes Not Discharged
1—A ................Ammonia stripping
1-13 ................ Dialysis
1—C ................ Diatomaceous earth filtration
1—D ................ Distillation
1—E ................ Electrodialysis
1—F................. Evaporation
1—G ................ Flocculation
1—H ................ Flotation
1-1.................. Foam fractionation
1—J ................. Freezing
1—K ................ Gas —phase separation
1—L.................Grinding (comminutors)
2—A ................ Carbon adsorption
2-13
................ Chemical oxidation
2—C
................ Chemical precipitation
2—D
................ Coagulation
2—E
................ Dechlorination
2—F.................
Disinfection (chlorine)
1. PHYSICAL TREATMENT PROCESSES
1—M ................ Grit removal
1—N ................Microstraining
1-0 ................ Mixing
1—P.................Moving bed filters
1—Q ................Multimedia filtration
1—R ................Rapid sand filtration
1—S.................Reverse osmosis (hypenrltration)
1—T................. Screening
1—U ................Sedimentation (settling)
1—V.................Slow sand filtration
1—W................ Solvent extraction
1—X................. Sorption
2. CHEMICAL TREATMENT PROCESSES
2—G................Disinfection (ozone)
2—H ................Disinfection (other)
2-1..................Electrochemical treatment
2—J .................Ion exchange
2—K................. Neutralization
2—L ................. Reduction
3. BIOLOGICAL TREATMENT PROCESSES
3—A ................Activated
sludge
3—E.................
Pre -aeration
3-13 ................Aerated
lagoons
3—F.................
Spray irrigation/land application
3—C ................Anaerobic
treatment
3—G ................Stabilization
ponds
3—D ................
Nitrification—denitrification
3—H ................Trickling
filtration
4. WASTEWATER DISPOSAL PROCESSES
4—A ................
Discharge to surface water
4—C ................
Reuse/recycle of treated effluent
4—B ................Ocean
discharge to outfall
4—D ................Underground
injection
5.
SLUDGE TREATMENT AND DISPOSAL
PROCESSES
5—A ................Aerobic
digestion
5—M ................
Heat drying
5-13 ................Anaerobic
digestion
5—N ................Heat
treatment
5—C ................
Belt filtration
5-0 ................Incineration
5—D ................
Centrifugation
5—P.................
Land application
5—E ................
Chemical conditioning
5—Q ................Landfill
5—F.................
Chlorine treatment
5—R ................Pressure
filtration
5—G ................
Composting
5—S .................
Pyrolysis
5—H ................
Drying beds
5—T .................
Sludge lagoons
5—I..................
Elutriation
5—U ................Vacuum
filtration
5—J .................
Flotation thickening
5—V.................
Vibration
5—K ................
Freezing
5—W................
Wet oxidation
5—L.................
Gravity thickening
2C-9

Exhibit 2C-3. Testing Requirements for Organic Toxic Pollutants Industry Categories*
INDUSTRY CATEGORY
Adhesives and sealants........................................................
Aluminum forming.................................................................
Auto and other laundries.......................................................
Battery manufacturing...........................................................
Coalmining...........................................................................
Coilcoating...........................................................................
Copperforming.....................................................................
Electric and electronic compounds .......................................
Electroplating........................................................................
Explosives manufacturing.....................................................
Foundries..............................................................................
Gum and wood chemicals (all subparts except D and F) .....
Gum and wood chemicals, Subpart D (tall oil rosin) .............
Gum and wood chemicals, Subpart F (rosin -based
derivatives)...........................................................................
Inorganic chemicals manufacturing ......................................
Iron and steel manufacturing ................................................
Leather tanning and finishing
Mechanical products manufacturing .....................................
Nonferrous metals manufacturing .........................................
Ore mining, Subpart B (base and precious metals) ..............
Organic chemicals manufacturing ........................................
Paint and ink formulation......................................................
Pesticides.............................................................................
Petroleumrefining.................................................................
Pharmaceutical preparations ................................................
Photographic equipment and supplies ..................................
Plastic and synthetic materials manufacturing ......................
Plastic processing.................................................................
Printing and publishing.........................................................
Pulp and paperboard mills ....................................................
Rubber processing................................................................
Soap and detergent manufacturing ......................................
Steam electric power plants ..................................................
Textile mills (except Subpart C, Greige Mills) .......................
Timber products processing .................................................
GC/MS FRACTIONt
Volatile
Acid
Base/Neutral
Pesticide
X
X
X
❑
X
X
X
❑
X
X
X
X
X
❑
X
❑
❑
❑
❑
❑
X
X
X
❑
X
X
X
❑
X
X
X
X
X
X
X
❑
❑
X
X
❑
X
X
X
❑
X
X
❑
❑
X
X
X
❑
X
X
X
❑
X
X
X
❑
X
X
X
❑
X
X
X
❑
X
X
X
❑
X
X
X
X
❑
X
❑
❑
X
X
X
X
X
X
X
❑
X
X
X
X
X
❑
❑
❑
X
X
X
❑
X
X
X
❑
X
X
X
X
X
❑
❑
❑
X
X
X
X
X
X
X
X
X
X
X
❑
X
X
X
❑
X
X
❑
❑
X
X
X
❑
X
X
X
X
See note at conclusion of 40 CFR 122, Appendix D (1983) for explanation of effect of suspensions on testing requirements for primary
industry categories.
t The pollutants in each fraction are listed in Table B.
X = Testing is required.
1= Testing is not required.
2C-10

Exhibit 2C-4. Hazardous Substances
1. Acetaldehyde
73. Captan
144. Ferrous sulfate
2. Acetic add
74. Carbaryl
145. Formaldehyde
3. Acetic anhydride
75. Carbofuran
146. Formic add
4. Acetone cyanohydrin
76. Carbon disulfide
147. Fumaric add
5. Acetyl bromide
77. Carbon tetrachloride
148. Furfural
6. Acetyl chloride
78. Chlordane
149. Guthion
7. Akxolein
79. Chlorine
150. Heptachlor
8. Acrylonitrile
80. Chlorobenzene
151. Hexachlorocydopentadiene
9. Adipic add
81. Chloroform
152. Hydrochloric add
10. Aldrin
82. Chloropyrifos
153. Hydrofluoric add
11. Allyl alcohol
83. Chlorosulfonic add
154. Hydrogen cyanide
12. Allyl chloride
84. Chromic acetate
155. Hydrogen sulfide
13. Aluminum sulfate
85. Chromic add
156. Isoprene
14. Ammonia
86. Chromic sulfate
157. Isopropanolamine dodecylbenzenesulfonate
15. Ammonium acetate
87. Chromous chloride
158. Kelthane
16. Ammonium benzoate
88. Cobaltous bromide
159. Kepone
17. Ammonium bicarbonate
89. Cobaltous formate
160. Lead acetate
18. Ammonium bichromate
90. Cobaltous sulfamate
161. Lead arsenate
19. Ammonium bifluoride
91. Coumaphos
162. Lead chloride
20. Ammonium bisulfite
92. Cresol
163. Lead fluoborate
21. Ammonium carbamate
93. Crotonaldehyde
164. Lead fluorite
22. Ammonium carbonate
94. Cupric acetate
165. Lead iodide
23. Ammonium chloride
95. Cupric acetoarsenite
166. Lead nitrate
24. Ammonium chromate
96. Cupric chloride
167. Lead stearate
25. Ammonium citrate
97. Cupric nitrate
168. Lead sulfate
26. Ammonium fluoroborate
98. Cupric oxalate
169. Lead sulfide
27. Ammonium fluoride
99. Cupric sulfate
170. Lead thiocyanate
28. Ammonium hydroxide
100. Cupric sulfate ammoniated
171. Lindane
29. Ammonium oxalate
101. Cupric tartrate
172. Lithium chromate
30. Ammonium silicofluoride
102. Cyanogen chloride
173. Malathion
31. Ammonium sulfamate
103. Cydohexane
174. Maleic add
32. Ammonium sulfide
104. 2,4-D add (2,4 dichlorophenoxyacetic acid)
175. Maleic anhydride
33. Ammonium sulfite
105. 2,4-D esters (2,4-dichlorophenoxyacetic acid esters)
176. Mercaptodimethur
34. Ammonium tartrate
106. DDT
177. Mercuric cyanide
35. Ammonium thiocyanate
107. Diazinon
178. Mercuric nitrate
36. Ammonium thiosulfate
108. Dicamba
179. Mercuric sulfate
37. Amyl acetate
109. Dichlobenil
180. Mercuric thiocyanate
38. Aniline
110. Dichlone
181. Mercurous nitrate
39. Antimony pentachloride
111. Dichlorobenzene
182. Methoxychlor
40. Antimony potassium tartrate
112. Dichloropropene
183. Methyl mercaptan
41. Antimony tribromide
113. Dichloropropene
184. Methyl methacrylate
42. Antimony tichloride
114. Dichloropropene-dichloproropane mix
185. Methyl parathion
43. Antimony trifluoride
115.2,2-dichloropropionic acid
186. Mevinphos
44. Antimony trioxide
116. Dichlorvos
187. Mexacarbate
45. Arsenic disulfide
117. Dieldrin
188. Monoethylamine
46. Arsenic pentoxide
118. Diethylamine
189. Monomethylamine
47. Arsenic tichlorde
119. Dimethylamine
190. Naled
48. Arsenic trioxide
120. Dinitrobenzene
191. Naphthalene
49. Arsenic tsulfide
121. Dinitrophenol
192. Naphthenic acid
50. Barium cyanide
122. Dinitrotoluene
193. Nickel ammonium sulfate
51. Benzene
123. Diquat
194. Nickel chloride
52. Benzoic acid
124. Disulfoton
195. Nickel hydroxide
53. Benzonitrile
125. Diuron
196. Nickel nitrate
54. Benzoyl chloride
126. Dodecylbenzesulfonic acid
197. Nickel sulfate
55. Benzyl chloride
127. Endosulfan
198. Nitric add
56. Beryllium chloride
128. Endrin
199. Nitrobenzene
57. Beryllium fluoride
129. Epichlorohydrin
200. Nitrogen dioxide
58. Beryllium nitrate
130. Ethion
201. Nitrophenol
59. Butylacetate
131. Ethylbenzene
202. Nitrotoluene
60. n-butylphthalate
132. Ethylenediamine
203. Paraformaldehyde
61. Butylamine
133. Ethylene dibromide
204. Parathion
62. Butyric add
134. Ethylene dichloride
205. Pentachlorophenol
63. Cadmium acetate
135. Ethylene diaminetetracetic add (EDTA)
206. Phenol
64. Cadmium bromide
136. Ferric ammonium citrate
207. Phosgene
65. Cadmium chloride
137. Ferric ammonium oxalate
208. Phosphoric acid
66. Calcium arsenate
138. Ferric chloride
209. Phosphorus
67. Calcium arsenite
139. Ferric fluoride
210. Phosphorus oxychloride
68. Calcium carbide
140. Ferric nitrate
211. Phosphorus pentasulfide
69. Calcium chromate
141. Ferric sulfate
212. Phosphorus tichlorde
70. Calcium cyanide
142. Ferrous ammonium sulfate
213. Polychlorinated biphenyls (PCB)
71. Calcium dodecylbenzenesulfonate
143. Ferrous chloride
214. Potassium arsenate
72. Calcium hypochlorite
215. Potassium arsenite
2C-11

Exhibit 2C-4. Hazardous Substances
216. Potassium bichromate
245. Sodium phosphate (dibasic)
271. Uranyl acetate
217. Potassium chromate
246. Sodium phosphate (tribasic)
272. Uranyl nitrate
218. Potassium cyanide
247. Sodium selenite
273. Vanadium penoxide
219. Potassium hydroxide
248. Strontium chromate
274. Vanadyl sulfate
220. Potassium permanganate
249. Strychnine
275. Vinyl acetate
221. Propargite
250. Styrene
276. Vinylidene chloride
222. Propionic acid
251. Sulfuric acid
277. Xylene
223. Propionic anhydride
252. Sulfur monochloride
278. Xylenol
224. Propylene oxide
253. 2,4,5-T acid (2,4,5-trichlorophenoxyacetc acid)
279. Zinc acetate
225. Pyrethrins
254. 2,4,5-T amines (2,4,546chlorophenoxy acetic acid
280. Zinc ammonium chloride
226. Quinoline
amines)
281. Zinc borate
227. Resorcinol
255. 2,4,5-T esters (2,4,5-hichlorophenoxy acetic acid
282. Zinc bromide
228. Selenium oxide
esters)
283. Zinc carbonate
229. Silver nitrate
256.2,4,5-T salts (2,4,5-trichlorophenoxy acetic acid salts)
284. Zinc chloride
230. Sodium
257. 2,4,5-TP acid (2,4,5-bichlorophenoxy propanoic acid)
285. Zinc cyanide
231. Sodium arsenate
258. 2,4,5-TP acid esters (2,4,5-bichlorophenoxy propanoic
286. Zinc fluoride
232. Sodium arsenite
add esters)
287. Zinc formate
233. Sodium bichromate
259. TDE (tetrachlorodiphenyl ethane)
288. Zinc hydrosulfite
234. Sodium bifluoride
260. Tetraethyl lead
289. Zinc nitrate
235. Sodium bisulfite
261. Tetraethyl pyrophosphate
290. Zinc phenolsulfonate
236. Sodium chromate
262. Thallium sulfate
291. Zinc phosphide
237. Sodium cyanide
263. Toluene
292. Zinc silicofluoride
238. Sodium dodecylbenzenesulfonate
264. Toxaphene
293. Zinc sulfate
239. Sodium fluoride
265. Trichlorofon
294. Zirconium nitrate
240. Sodium hydrosulfide
266. Trichloroethylene
295. Zirconium potassium fluoride
241. Sodium hydroxide
267. Trichlorophenol
296. Zirconium sulfate
242. Sodium hypochlorite
268. Triethanolamine dodecylbenzenesulfonate
297. Zirconium tetrachloride
243. Sodium methylate
269. Triethylamine
244. Sodium nitrite
270. Trimethylamine
2C-12

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP OMB No. 2040-0004
Form U.S. Environmental Protection Agency
2C ♦SOEPA Application for NPDES Permit to Discharge Wastewater
NPDES EXISTING MANUFACTURING, COMMERCIAL, MINING, AND SILVICULTURE OPERATIONS
SECTIONOUTFALL LOCATION
1.1 Provide information on each of the facility's outfalls in the table below.
o OutfaReceiving Water Name Latitude Longitude
Number
0 001 McDowell Creek 35' 25' 42' 8f 53' ff
J
R
0
SECTION
D' 1
a, 2.1 Have you attached a line drawing to this application that shows the water flow through your facility with a water
v .3 balance? (See instructions for drawing requirements. See Exhibit 2C-1 at end of instructions for example.)
J R
o ❑✓ Yes ❑ No
SECTION• 1
3.1 For each outfall identified under Item 1. 1, provide average flow and treatment information. Add additional sheets if
necessary.
**Outfall Number** 001
Operations
Operation Average Flow
Treatment of surface water for potability 19 mgd
c
E Backwash water .294 mgd
m
m
Sedimentation Basin Blowdowns .300 mgd
c
`U) Dechlorination ahead of discharge to outfall 1 .554 mgd
3
0 Treatment Units
U-
a, Description Code fFinal Disposal of Solid or
from (include size, flow rate through each treatment unit, Table ro Liquid Wastes Other Than
a retention time, etc.) by Discharge
Flocculator Basins 1 G See Line Drawing
Sedimentation Basins 1 u See Line Drawing
Conventional Filtration (Sand and Anthracite) 1 Q See Line Drawing
Equalization Basin (Backwash Storage) N/A See Line Drawing
RECEIVED
SEP 0 4 2024
NCDEQ/DWR/NPDES
EPA Form 3510-2C (Revised 3-19) Page 1

EPA Identification Number
NPDES Permit Number
Facility Name
Form Approved 03/05/19
100000062601
NCO084387
Dukes WTP
OMB No. 2040-0004
3.1
**Outfall Number** 001
cont.
Operations Contributing to Flow
Operation Average Flow
mgd
mgd
mgd
mgd
Treatment
Description
Units
Code from
Final Disposal of Solid or
(include size, flow rate through each treatment unit,
Table 2C-1
Liquid Wastes Other Than
retention time, etc.)
by Dischar e
Gravity Thickener
5 L
See Line Drawing
c
Clarifier
N/A
See Line Drawing
0
U
c
Dechlorination Chamber
2 E
See Line Drawing
m
E
dSludge
Lagoon
5 T
See Line Drawing
**Outfall Number"
R
Operations
Operation
Average Flow
0
U-
a,
mgd
R
N
a'
mgd
mgd
mgd
Treatment
Description
Units
Code from
Final Disposal of Solid or
(include size, flow rate through each treatment unit,
Table 2C-1
Liquid Wastes Other Than
retention time, etc.)
by Discharge
3.2
Are you applying for an NPDES permit to operate a privately owned treatment works?
d
❑ Yes ❑✓ No 4 SKIP to Section 4.
Nin
3.3
Have you attached a list that identifies each user of the treatment works?
❑ Yes ❑ No
EPA Form 3510-2C (Revised 3-19) Page 2

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP OMB No. 2040-0004
SECTION• 1
4.1 Except for storm runoff, leaks, or spills, are any discharges described in Sections 1 and 3 intermittent or seasonal?
❑✓ Yes ❑ No 4 SKIP to Section 5.
4.2
Provide information
on intermittent or seasonal flows for each applicable outfall.
Attach additional pages, if n cessary.
Frequency
Flow
Rate
Outfall
Operation
Duration
Average
Average
Long -Term
Maximum
Number
(list)
Days/Week
MonthsNear
Average
Dail
Decant from Lagon
1 days/week
12 months/year
0.05 mgd
0.10 mgd
52 days
0
001
days/week
months/year
mgd
mgd
days
days/week
months/year
mgd
mgd
days
E
days/week
months/year
mgd
mgd
days
a�
c
days/week
months/year
mgd
mgd
days
days/week
months/year
mgd
mgd
days
days/week
months/year
mgd
mgd
days
days/week
months/year
mgd
mgd
days
days/week months/year mgd mgd days
SECTION•'•D
• I
Do any effluent limitation guidelines (ELGs) promulgated by EPA under Section 304 of the CWA apply to your facility?
5.1
❑ Yes ❑✓ No 4 SKIP to Section 6.
U)
5.2
Provide the following information on applicable ELGs.
ELG Category
ELG Subcategory
Regulatory Citation
w
d
.n
R
U
n
a.
a
5.3
Are any of the applicable ELGs expressed in terms of production (or other measure of operation)?
❑ Yes ❑ No 4 SKIP to Section 6.
0
;g
5.4
Provide an actual measure of daily production expressed in terms and units of applicable ELGs.
J
a
Outfall
Number
Operation, Product, or Material
Quantity per Day
Unit of
Measure
d
R
m
0
.;
0
a
EPA Form 3510-2C (Revised 3-19) Page 3

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP OMB No. 2040-0004
SECTION'• 1
6.1 Are you presently required by any federal, state, or local authority to meet an implementation schedule for constructing,
upgrading, or operating wastewater treatment equipment or practices or any other environmental programs that could
affect the discharges described in this application?
❑ Yes ❑✓ No 4 SKIP to Item 6.3.
6.2
Briefly identify each applicable project in the table below.
Affected
Final Compliance Dates
E
Brief Identification and Description of
Outfalls
Source(s) of
c
Project
(list outfall
Discharge
Required
Projected
a,
number
E
-c
c
m
m
ai
M
m
z
rn
a
6.3
Have you attached sheets describing any additional water pollution control programs (or other environmental projects
that may affect your discharges) that you now have underway or planned? (optional item)
❑ Yes ❑ No ✓❑ Not applicable
SECTIONi
See the instructions to determine the pollutants and parameters you are required to monitor and, in turn, the tables you must
complete. Not all applicants need to complete each table.
Table A. Conventional and Non -Conventional Pollutants
7.1
Are you requesting a waiver from your NPDES permitting authority for one or more of the Table A pollutants for any of
your outfalls?
❑✓ Yes ❑ No 4 SKIP to Item 7.3.
7.2
If yes, indicate the applicable outfalls below. Attach waiver request and other required information to the application.
Outfall Number 001 Outfall Number Outfall Number
7.3
Have you completed monitoring for all Table A pollutants at each of your outfalls for which a waiver has not been
N
requested and attached the results to this application package?
❑✓ Yes ❑ No; a waiver has been requested from my NPDES
permitting authority for all pollutants at all outfalls.
cc
Table B.
Toxic Metals, Cyanide, Total Phenols, and Organic Toxic Pollutants
Y
7.4
Do any of the facility's processes that contribute wastewater fall into one or more of the primary industry categories
listed in Exhibit 2C-3? (See end of instructions for exhibit.)
❑ Yes ❑✓ No 4 SKIP to Item 7.8.
R
7.5
Have you checked "Testing Required" for all toxic metals, cyanide, and total phenols in Section 1 of Table B?
w
❑ Yes ❑ No
7.6
List the applicable primary industry categories and check the boxes indicating the required GC/MS fraction(s) identified
in Exhibit 2C-3.
Primary Industry Category
Required GC/MS Fraction(s)
Check applicable boxes.
❑ Volatile ❑ Acid ❑ Base/Neutral ❑ Pesticide
❑ Volatile ❑ Acid ❑ Base/Neutral ❑ Pesticide
❑ Volatile ❑ Acid ❑ Base/Neutral ❑ Pesticide
EPA Form 3510-2C (Revised 3-19) Page 4

EPA Identification Number
NPDES Permit Number
Facility Name
Form Approved 03/05/19
100000062601
NCO084387
Dukes WTP
OMB No. 2040-0004
7.7
Have you checked "Testing Required" for all required pollutants in Sections 2 through 5 of Table B for each of the
GC/MS fractions checked in Item 7.6?
❑ Yes ❑ No
7.8
Have you checked 'Believed Present' or "Believed Absent' for all pollutants listed in Sections 1 through 5 of Table B
where testing is not required?
© Yes ❑ No
7.9
Have you provided (1) quantitative data for those Section 1, Table B, pollutants for which you have indicated testing is
required or (2) quantitative data or other required information for those Section 1, Table B, pollutants that you have
indicated are 'Believed Present' in your discharge?
❑ Yes ❑✓ No
7.10
Does the applicant qualify for a small business exemption under the criteria specified in the instructions?
❑ Yes 4 Note that you qualify at the top of Table B, ❑ No
then SKIP to Item 7.12.
S
7.11
Have you provided (1) quantitative data for those Sections 2 through 5, Table B, pollutants for which you have
c
determined testing is required or (2) quantitative data or an explanation for those Sections 2 through 5, Table B,
L'
pollutants you have indicated are "Believed Present' in your discharge?
U)
U)
❑✓ Yes ❑ No
d
Table C. Certain Conventional and Non -Conventional Pollutants
2
7.12
Have you indicated whether pollutants are 'Believed Present' or "Believed Absent' for all pollutants listed on Table C
s
for all outfalls?
Y
❑✓ Yes ❑ No
z
7.13
Have you completed Table C by providing (1) quantitative data for those pollutants that are limited either directly or
indirectly in an ELG and/or (2) quantitative data or an explanation for those pollutants for which you have indicated
'Believed Present'?
d
❑✓ Yes ❑ No
W
Table D. Certain Hazardous Substances and Asbestos
7.14
Have you indicated whether pollutants are `Believed Present' or 'Believed Absent' for all pollutants listed in Table D for
all outfalls?
❑✓ Yes ❑ No
7.15
Have you completed Table D by (1) describing the reasons the applicable pollutants are expected to be discharged
and (2) by providing quantitative data, if available?
❑ Yes ❑✓ No
Table E. 2,3,7,8-Tetrachlorodibenzo- -Dioxin 2,3,7,8-TCDD
7.16
Does the facility use or manufacture one or more of the 2,3,7,8-TCDD congeners listed in the instructions, or do you
know or have reason to believe that TCDD is or may be present in the effluent?
❑ Yes -+ Complete Table E. ❑✓ No 4 SKIP to Section 8.
7.17
Have you completed Table E by reporting qualitative data for TCDD?
❑ Yes ❑ No
SECTIONOR
MANUFACTURED TOXICS
Is any pollutant listed in Table B a substance or a component of a substance used or manufactured at your facility as
8.1
an intermediate or final product or byproduct?
❑ Yes ❑✓ No -+ SKIP to Section 9.
8.2
List the pollutants below.
c —
M 0
1. 4. 7.
0
2. 5. 8.
3. 6. 9.
EPA Form 3510-2C (Revised 3-19) Page 5

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP OMB No. 2040-0004
SECTION• • • •
9.1 Do you have any knowledge or reason to believe that any biological test for acute or chronic toxicity has been made
within the last three years on (1) any of your discharges or (2) on a receiving water in relation to your discharge?
❑✓ Yes ❑ No 4 SKIP to Section 10.
y
U)
0
,°7
9.2
Identify the tests and their
Durposes below.
Test(s)
Purpose of Test(s)
Submitted to NPDES
Date Submitted
x
Permitting Authority?
0
F'
Whole Effluent Toxicity
Determine if water is
El Yes ❑ NO
M
Testing Utilzing
negatively impacting
0
M
'
m
❑ Yes El No
❑ Yes ❑ No
SECTION•
Were any of the analyses reported in Section 7 performed by a contract laboratory or consulting firm?
10.1
❑ Yes ❑✓ No 4 SKIP to Section 11.
10.2
Provide information for each contract laboratory or consulting
firm below.
Laboratory Number 1
Laboratory Number 2
Laboratory Number 3
N
Name of laboratory/firm
Laboratory address
a
0
Phone number
Pollutant(s) analyzed
SECTIONDD
• •- •
Has the NPDES permitting authority requested additional information?
11.1
❑ Yes ❑✓ No 4 SKIP to Section 12.
0
11.2
List the information requested and attach it to this application.
`o
1 Email Correspndance with NCDEQ 4.
m
c
0
a
2. 5.
a
3. 6.
EPA Form 3510-2C (Revised 3-19) Page 6

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP OMB No. 2040-0004
SECTION•
12.1
1
In Column 1 below, mark the sections of Form 2C that you have completed and are submitting with your application.
For each section, specify in Column 2 any attachments that you are enclosing to alert the permitting authority. Note
that not all applicants are required to com lete all sections or provide attachments.
Column 1
Column 2
❑✓ Section 1: Outfall Location
❑✓ w/ attachments
❑✓ Section 2: Line Drawing
✓❑ w/ line drawing ❑ w/ additional attachments
w/ list of each user of
Section 3: Average Flows and
❑ w/ attachments ❑ privately owned treatment
Treatment
works
❑✓ Section 4: Intermittent Flows
❑ w/ attachments
❑✓ Section 5: Production
❑ w/ attachments
w/ optional additional
❑✓ Section 6: Improvements
❑ w/ attachments ❑ sheets describing any
additional pollution control
tans
❑ w/ request for a waiver and ❑ wl explanation for identical
supporting information outfalls
d
w/ small business exemption
El ❑ w/ other attachments
d
request
N
❑ Section 7: Effluent and Intake
❑✓ w/ Table A ❑✓ w/ Table B
=
Characteristics
❑✓ w/ Table C ❑✓ w/ Table D
d
✓ w/ Table E w/ analytical results as an
❑ ❑
0
attachment
❑ Section 8: Used or Manufactured
❑ w/ attachments
y
Toxics
❑ Section 9: Biological Toxicity
❑ w/ attachments
t
Tests
U
✓❑ Section 10: Contract Analyses
❑ w/ attachments
✓❑ Section 11: Additional Information
❑✓ w/ attachments
❑ Section 12: Checklist and
❑ w/ attachments
Certification Statement
12.2
Certification Statement
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in
accordance with a system designed to assure that qualified personnel properly gather and evaluate the information
submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly
responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true,
accurate, and complete. I am aware that there are significant penalties for submitting false information, including the
possibility of fine and imprisonment for knowing violations.
Name (print or type first and last name)
Official title
Gabe Sasser
eMr n
Signature
a si e
'9k� S F P
#/4o/2024
NCDEQ/DWR/NPDES
EPA Form 3510-2C (Revised 3-19) Page 7

This page intentionally left blank.

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP 001 OMB No.2040-0004
TABLE A. CONVENTIONAL AND NON
CONVENTIONAL•
1
Effluent
Intake
Pollutant
Waiver
Requested
Units
(specify)
0 6onal
Maximum
Daily
Maximum
Monthly
Long -Term
Average Daily
Number of
Long -Term
Number of
(if applicable)
Discharge
Discharge
Discharge
Analyses
Average Value
Analyses
re ui ed
d available
if available
❑ Check here if you have applied to your NPDES permitting authority for a waiver for all of the pollutants listed on this table for the noted outfall.
1
Biochemical oxygen demand
❑
Concentration
N/A
Mass
N/A
(BOD5)
2.
Chemical oxygen demand
❑
Concentration
N/A
Mass
N/A
(COD)
Concentration
N/A
3.
Total organic carbon (TOC)❑
Mass
N/A
Concentration
mg/L
9
4.41
187
4.
Total suspended solids (TSS)
❑
Mass
Concentration
mg/L
0.23
.15
16
5.
Ammonia (as N)
❑
Mass
6.
Flow
❑
Rate
MGD
1.52
0.55
1136
Temperature (winter)
❑
°C
°C
20
15
60
7.
Temperature (summer)
❑
°C
°C
33
24
127
pH (minimum)
❑
Standard units
S.U.
7.5
7.08
187
8.
pH (maximum)
❑
Standard units
S.U.
i Sampling shall be conducted according to sumclently sensitive test procedures (i.e., metnoas) approved under au urK isu Tor me analysis or pollutants or polwtant parameters or
required under 40 CFR chapter I, subchapter N or 0. See instructions and 40 CFR 122.21(e)(3).
EPA Form 3510-2C (Revised 3-19) Page 9

This page intentionally left blank.

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP 001 OMB No.2040-0004
TOXICTABLE B. 1E,
TOTAL PHENOLS,1
ORGANIC TOXIC••
4I
Presence or Absence
Intake
checkone
Effluent
(optional)
Pollutant/Parameter
Testing
Units
Maximum
Maximum
Long -Term
Long -
(and CAS Number, if available)
Required
Believed
Believed
(specify)
Daily
Monthly
Average
Number
Term
Number
Present
Absent
Discharge
Discharge
Daily
chare
Discharge
of
Analyses
Average
of
Analyses
(required)
(if available)
if available
Value
Check here if you qualify as a small business per the instructions to Form 2C and, therefore, do not need to submit quantitative data for any of the organic toxic pollutants in Sections
2 through 5 of this table. Note, however, that you must still indicate in the appropriate column of this table if you believe any of the pollutants listed are present in your discharge.
Section 1. Toxic Metals, Cyanide, and Total Phenols
1.1
Antimony, total
❑�
Concentration
Mass
(7440-36-0)
1.2
Arsenic, total
❑
❑
IZI
Concentration
Mass
(7440-38-2)
1.3
Beryllium, total
El
El
Concentration
Mass
(7440-41-7)
14
Cadmium, total
❑
❑
❑
Concentration
Mass
(744043-9)
1.5
Chromium, total
Concentration
Mass
(7440-47-3)
1.6
Copper, total
11
El
El
Concentration
Mass
(7440-50-8)
1.7
Lead total
❑❑
Concentration
Mass
(7439-92-1)
1.8
Mercury, total
❑
11❑
Concentration
Mass
(7439-97-6)
1.9
Nickel, total
Concentration
Mass
(7440-02-0)
1.10
Selenium, total
El
1:1
El
Concentration
Mass
(7778249-2)
1.11
Silver, total
Concentration
1 Mass
(7440-224)
EPA Form 3510-2C (Revised 3-19) Page 11

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05A9
100000062601 NCO084387 Dukes WTP 001 OMB No.2040-00"
Presence or Absence
Intake
check one
Effluent
(optional)
PollutantlParameter
Testing
Units
Maximum
Maximum
Long -Term
Long -
(and CAS Number, it available)
Required
Believed
Believed
(specify)
Daily
Monthly
Average
Number
Term
Number
Present
Absent
Discharge
Discharge
Daily
Discharge
of
Analyses
Average
of
Analyses
(required)
(if available)
f available
Value
1.12
Thallium, total
❑
❑
❑
Concentration
Mass
(7440-28-0)
1.13
Zinc total
❑
❑
❑
Concentration
Mass
(7440-66-6)
1.14
Cyanide, total
❑
❑
❑
Concentration
Mass
(57-12-5)
1.15
Phenols, total
❑
❑
❑
Concentration
Mass
Section 2.Organic Toxic Pollutants (GCIMS Fraction —Volatile Compounds)
2.1
Acrolein
El
El
Concentration
Mass
(107-02-8)
2.2
Acrylonitrile
❑
❑
©
Concentration
Mass
(107-13-1)
2.3
Benzene
❑
❑
❑
Concentration
Mass
(71-43-2)
2.4
Bromoform
❑
❑
❑
Concentration
Mass
(75-25-2)
2.5
Carbon tetrachloride
❑
❑
❑✓
Concentration
Mass
(56-23-5)
2.6
Chlorobenzene
❑
❑
❑
Concentration
Mass
(108-90-7)
27
Chlorodibromomethane
❑
❑
El
Concentration
Mass
(124-48-1)
2.8
Chloroethane
❑
❑
❑
Concentration
Mass
(75-00-3)
EPA Form 3510-2C (Revised 3-19) Page 12

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03105/19
W
100000062601 NCO084387 Dukes rP 001 OMB No. 2040MM
TABLE B. TOXIC METALS, CYANIDE,
TOTAL PHENOLS,
AND ORGANIC TOXIC
Presence or Absence
POLLUTAN I Z) (40 CFR
122.21(g)(7)(v))'
Intake
check one
Effluent
(optional)
PollutantlParameter
(and CAS Number, it available)
Testing
Required
Believed
Believed
Units
(specify)Maximum
Maximum
Long -Term
Average
Number
Lon -
9fy)
Term
Number
Present
Absent
Daily
Discharge
Monthly
Df charg
Dail
of
Average
of
(required)if
Discharge
Analyses
Analyses
I
available
2.9 2-chloroethylvinyl ether
El
❑
❑✓
Concentration
Mass
(110-75-8)
2.10 Chloroform (67-66-3)
❑
❑
0
Concentration
Mass
211 Dichlorobromomethane
El
❑
❑
Concentration
Mass
(75-27-4)
212 1 1-dichlorcethane
Concentration
Mass
(75-34-3)
213 12-dichlorcethane
El
11
©
Concentration
Mass
(107-06-2)
214
1 1-dichlorcethylene
Concentration
Mass
(75-35-4)
215
12-dichloropropane
❑
❑
❑
Concentration
Mass
(78-87-5)
216
13-dichloropropylene
Concentration
Mass
(542-75-6)
2.17
Ethylbenzene
©
Concentration
Mass
(100-41-4)
218
Methyl bromide
Concentration
Mass
(74-83-9)
219 Methyl chloride
Concentration
Mass
(74-87-3)
2.20 Methylene chloride
❑
❑
El
Concentration
Mass
(75-09-2)
r221
1 1 2 2- tetrachloroethane
❑
❑
Concentration
Mass
(79-34-5)
EPA Form 3510-2C (Revised 3.19) Page 13

EPA Identification Number NPDES Perrnit Number Facility Name Ouffall Number Form Approved 03105/19
100000062601 NCO084387 Dukes WTP 001 OMB No.2040-0004
TOXICTABLE B.
•E,
TOTAL PHENOLS,AND
ORGANIC
Presence or Absence
• POLLUTANTSI
Intake
check one
Effluent
(optional)
Pollutant/Parameter
(and CAS Number, if available)
Testing
Required
q
Believed Believed
Units
(specify)
Maximum
Maximum
Long -Term
Average
9
Number
Long-
Term
Number
Present Absent
Daily
Discharge
Monthly
Discharge
Daily
of
Average
of
(required)
(if available)
Discharge
Analyses
Value
Analyses
if available
2.22
Tetrachloroethylene
Concentration
Mass
(127-18-4)
2.23
Toluene
❑
El
El
Concentration
Mass
(108-88-3)
2.24
12-trans-dichloroethylene
❑
❑
[a
Concentration
Mass
(156.60-5)
2.25
1 1,1-trichloroethane
El
El
ElConcentration
Mass
(71 55-6)
2.26
1 1 2-trichloroethane
El
0
Concentration
Mass
(79-00-5)
2.27
Trichloroethylene
❑
❑
Concentration
Mass
(79-01-6)
2.28
Vinyl chloride
Concentration
Mass
(75-01-4)
Section 3. Organic Toxic Pollutants (GCIMS Fraction —Acid Compounds)
3.1
2-chlorophenol
El
0
Concentration
Mass
(95-57-8)
3.2
2 4-dichlorophenol
El
El
Concentration
Mass
(120-83-2)
3.3
24-dimethylphenol
❑
❑
El
Concentration
Mass
(105-67-9)
3.4
4 6-dinitro-o-cresol
El
❑
❑✓
Concentration
Mass
(534-52-1)
3.5
2 4-dinitrophenol
❑
El
✓❑
Concentration
Mass
(51-28-5)
EPA Form 3510-2C (Revised 3-19) Page 14

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP 001 OMB No.2040-0004
� �
rr
rr � rr -
rr • � r
Presence or Absence
Intake
check one
Effluent
(optional)
PollutantlParameter
Testing
Units
Maximum
Maximum
Long -Tenn
Long -
(and CAS Number, if available)
Required
Believed
Believed
(specify)
Daily
Monthly
Average
Number
Term
Number
Present
Absent
Discharge
Discharge
Daily
Discharge
of
Analyses
Average
of
Analyses
(required)
(if available)
if available
Value
3.6
2-nitrophenol
❑
❑
0
Concentration
Mass
(88-75-5)
3.7
4-nitrophenol
❑
❑
✓❑
Concentration
Mass
(100-02-7)
3.8
p-chloro-m-cresol
❑
❑
✓❑
Concentration
Mass
(59-50-7)
3.9
Pentachlorophenol
❑
❑
❑
Concentration
Mass
(87-86-5)
3.10
Phenol
El
El
El
Concentration
Mass
(108-95-2)
311
2,4,6-hichlorophenol
❑
❑
❑
Concentration
Mass
(88-05-2)
Section 4. Organic Toxic Pollutants GCIMS Fraction —Base (Neutral Compounds
41
Acenaphthene
❑
❑
❑✓
Concentration
Mass
(83-32-9)
4.2
Acenaphthylene
El
❑�
Concentration
Mass
(208-96-8)
4.3
Anthracene
❑
0
✓❑
Concentration
Mass
(120-12-7)
4.4
Benzidine
❑
El
R1
Concentration
Mass
(92-87-5)
4.5
Benzo (a) anthracene
❑
❑
ID
Concentration
Mass
(56-55-3)
4.6
Benzo (a) pyrene
El
❑
Q
Concentration
Mass
(50-32-8)
EPA Form 3510-2C (Revised 3-19) Page 15

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05I19
300000062601 NCO084387 Dukes VJTP 001 OMB No, 2040-0004
Presence or Absence
Intake
check one
Effluent
(optional)
Pollutant/Parameter
Testing
Units
Maximum
Maximum
Long -Terre
Long-
(and CAS Number, if available)
Required
Believed
Believed
(specify)
Daily
Monthly
Average
Number
Term
Number
Present
Absent
Discharge
ischarge
0(ifavailable)
Daily
Discharge
of
Analyses
Average
of
Analyses
d available
4.7
3 4-benzofluoranthene
❑
❑
Q
Concentration
Mass
(205-99-2)
4.8
Benzo (ghi) perylene
❑
ElMass
Concentration
(191-24-2)
4.9
Benzo (k) fluoranthene
❑
❑
❑
Concentration
Mass
(207-08-9)
4.10
Bis (2-chloroethoxy) methane
❑
❑
❑
Concentration
Mass
(111-91-1)
411
Bis (2-chlorcethyl) ether
❑
❑
❑
Concentration
Mass
(111-44-4)
412
Bis (2-chloroisopropyl) ether
❑
❑
❑
Concentration
Mass
(102-80-1)
4.13
Bis (2-ethylhexyl) phthalate
❑
❑
Concentration
Mass
(117-81-7)
4.14
4-bromophenyl phenyl ether
❑
❑
❑
Concentration
Mass
(101-55-3)
4.15
Butyl benzyl phthalate
❑
❑
Concentration
Mass
(85-68-7)
4.16
2-chloronaphthalene
❑
❑
Concentration
Mass
(91-58-7)
417
4-chlorophenyl phenyl ether
Concentration
Mass
(7005-72-3)
4.18
Chrysene
❑
❑
Concentration
Mass
(218-01-9)
419
Dibenzo (a,h) anthracene
❑
❑
❑
Concentration
Mass
(53-70-3)
EPA Form 3510-2C (Revised 3-19) Page 16

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP 001 OMB No. 2040-0004
• •
•
• • ••
Presence or Absence
Intake
check one
Effluent
(optional)
Pollutant/Parameter
Testing
Units
Maximum
Maximum
Long -Term
Long•
(and CAS Number, if available)
Required
Believed
Believed
(specify)
Daily
Monthly
Average
Number
Term
Number
Present
Absent
Daily
Discharge
Analyses
Average
of
Analyses
(required)hard)
ifavailabllee)
if available
4.20
12-dichlorobenzene
❑
❑
❑
Concentration
Mass
(95-50-1)
4.21
1 3-dichlorobenzene
❑
❑
❑
Concentration
Mass
(541-73-1)
4.22
14-dichlorobenzene
❑
❑
Concentration
Mass
(10646-7)
4.23
3 3-dichlorobenzidine
❑
❑
❑
Concentration
Mass
(91-94-1)
4.24
Diethyl phthalate
❑
❑
©
Concentration
Mass
(84-66-2)
4.25
Dimethyl phthalate
❑
❑
❑✓
Concentration
Mass
(131-11-3)
4.26
Di-n-butyl phthalate
❑
❑
©
Concentration
Mass
(84-74-2)
427
24-dinitrotoluene
❑
❑
©
Concentration
Mass
(121-14-2)
4.28
2 6-dinitrotoluene
❑
❑
❑✓
Concentration
Mass
(606-20-2)
4.29
Di-n-octyl phthalate
❑
❑
❑
Concentration
Mass
(117-84-0)
4.30
1 ,2-DiphenAhydrazine
❑
❑
0
Concentration
Mass
(as azobenzene)(122-66-7)
4.31
Fluoranthene
❑
❑
0
Concentration
Mass
(206-44-0)
4.32
Fluorene
❑
❑
0
Concentration
Mass
(86-73-7)
EPA Form 3510-2C (Revised 3-19) Page 17

EPA Idenfificafion Number NPDES Pemwt Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP 001 OMB No.2040-0004
TABLE
B. •CYANIDE,•PHENOLS,1
ORGANIC TOXIC'•
Presence or Absence
I
Intake
check one
Effluent
(optional)
Pollutant/Parameter
Testing
Units
Maximum
Maximum
Long -Term
Long -Number
(and CAS Number, if available)
Required
Believed
Believed
(specify)
Daily
Monthly
Average
Number
Term
Present
Absent
Discharge
Discharge
Daily
Discharge
g
of
Analyses
Average
of
Analyses
(required)
(if available)
if available
Value
4.33
Hexachlorobenzene
El
Q
Concentration
Mass
(118-74-1)
4.34
Hexachlorobutadiene
El
❑
❑
Concentration
Mass
(87-68-3)
4.35
Hexachlorocyclopentadiene
Concentration
Mass
(7747-4)
4.36
Hexachloroethane
El
El
0
Concentration
Mass
(67-72-1)
4.37
Indeno (1,2,3-cd) pyrene
Concentration
Mass
(193-39-5)
4.38
Isophorone
El
El
Concentration
Mass
(78-59-1)
4.39
Naphthalene
❑
❑
✓❑
Concentration
Mass
(91-20-3)
4.40
Nitrobenzene
❑
El
❑
Concentration
Mass
(98-95-3)
4.41
N-nitrosodimethylamine
❑
❑
❑
Concentration
Mass
(62-75-9)
4.42
N-nitrosodi-n-propylamine
El
❑
R1
Concentration
Mass
(621-64-7)
4.43
N-nitrosodiphenylamine
❑
❑
0
Concentration
Mass
(86-30-6)
4'�
Phenanthrene
El
11
El
Concentration
Mass
(85-01-8)
r44Pyrene
©
Concentration
Mass
(129-00-0)
EPA Form 3510-2C (Revised 3-19) Page 18

EPA Identification Number NPDES Permit Number Facility Name WWI Number Form Approved 03/05119
100000062601 NCO084387 Dukes WTP 001 OMB No.2040-0004
MEW
Presence or Absence
Intake
check one
Effluent
(optional)
Pollutant/Parameter
(and CAS Number, if available)
Testing
Required
Believed
Believed
Units
(specify)
Maximum
Max
Maximum
Long-Terre
Number
-
Long -
Term
Number
Present
Absent
Daily
Daily
Discharge
Monthly
Discharge
Daily
of
Average
of
(required)
(favailable)
Discharge
Analyses
Value
Analyses
if available
4.146 1 2 4-trichlorobenzene
❑
❑
✓❑
Concentration
I Mass
(120-82-1)
I
I
Section 5. Organic Toxic Pollutants GCIMS Fraction —Pesticides
5.1
Aldrin
❑
❑
❑
Concentration
Mass
(309-00-2)
5.2
a-BHC
❑
❑
❑
Concentration
Mass
(319-84-6)
5.3
�-BHC
❑
❑
Concentration
Mass
(319-85-7)
5.4
y-BHC
❑
❑
❑
Concentration
Mass
(58-89-9)
5.5
S-BHC
❑
❑
❑
Concentration
Mass
(310-86-8)
5.6
Chlordane
❑
❑
Concentration
Mass
(57-74-9)
5.7
4 4'-DDT
❑
El
Concentration
Mass
(50-20-3)
5.8
44'-DDE
❑
ElMass
Concentration
(72-55-9)
5.9
44'-DDD
❑
❑
❑
Concentration
Mass
(72-54-8)
5.10
Dieldrin
❑
❑
Concentration
Mass
(60.57-1)
5.11
o-endosulfan
❑
ElConcentration
Mass
(115 -29-7)
EPA Form 3510-2C (Revised 3-19) Page 19

EPA Identification Number NPDESPermit Number Facility Name OuttallNumber Form Approved03/05/19
100000062601 NCO084387 Dukes VVTP 001 OMB No. 2040-0004
•
Pollutant/Parameter
(and CAS Number, if available)
•
Testing
Required
• 1 ••
Presence or Absence
check one
• •• I
Units
(specify)
Effluent
Intake
(optional)
Believed
Present
Believed
Absent
Maximum
Daily
Discharge
(required)
Maximum
Monthly
Discharge
(ifg
f available)
Long -Term
Average
Daily
Discharge
if available
Number
of
Analyses
Long -
Term
Average
Value
Number
of
Analyses
512
j -endosulfan
(115-29-7)
❑
❑
0
Concentration
Mass
5.13
Endosulfan suNate
(1031-07-8)
❑
❑
❑
Concentration
Mass
5.14
Endrin
p2 20 8)
El
❑
❑�
Concentration
Mass
5.15
Endrin aldehyde
(7421-93-0)
❑
❑
❑
Concentration
Mass
5.16
Heptachlor
(7�-8)
El
❑
❑
Concentration
Mass
5.17
Heptachlor epoxide
(1024-57-3)
❑
❑
❑
Concentration
Mass
5.18
PCB-1242
(53469-21-9)
❑
❑
0
Concentration
Mass
5.19
PCB-1254
(11097-69-1)
❑
❑
❑�
Concentration
Mass
5.20
PCB-1221
(11104-28-2)
❑
❑
IZI
Concentration
Mass
5.21
PCB-1232
(11141-16-5)
❑
❑
❑
Concentration
Mass
5.22
PCB-1248
(12672-29-6)
❑
0
Concentration
Mass
5.23
PCB-1260
(11096-82-5)
❑
❑
❑
Concentration
Mass
5.24
PCB-1016
(12674-11-2)
❑
Concentration
Mass
EPA Form 3510-2C (Revised 3-19) Page 20

EPA Identification Number NPDES Permit Number Facility Name Oudall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP 001 OMB No. 2040-0004
Presence or Absence
Intake
check one
Effluent
(optional)
Pollutant/Parameter
Testing
Units
Maximum
Maximum
Long•Term
(andCASNumber,'rfavailable)
Required
Believed
Believed
(specify)
Daily
Monthly
Average
NumberNumber
Present
Absent
Discharge
Discharge
Dail y
of
of
(required)
(if available)
Discharge
AnalysesAnalyses
LAverage
if available
Toxaphene
Concentration
5.25 (8001-35-2)
Mass
r Sampling shall be conducted according to sufficiently sensitive test procedures (i.e., methods) approved under 40 CFR 136 for the analysis of pollutants or pollutant parameters or
required under 40 CFR chapter I, subchapter N or 0. See instructions and 40 CFR 122.21(e)(3).
EPA Form 3510-2C (Revised 3-19) Page 21

This page intentionally left blank.

EPA Identfication Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP 001 OMB No.2040-0004
Intake
Effluent
(Optional)
7BIlevedBelieved
Pollutant
Units
(specify)
Maximum Daily
Maximum
Monthly
Long -Term
Average Daily
Number of
Long•Term
Number of
Discharge
Discharge
Discharge
Analyses
Average
Analyses
(required)
d available
if available
Value
❑ Check here if you believe all pollutants on Table C to be present in your discharge from the noted outfall. You need not complete the "Presence or Absence" column of Table C for
each pollutant.
❑ Check here if you believe all pollutants on Table C to be absent in your discharge from the noted outfall. You need not complete the 'Presence or Absence" column of Table C for
each pollutant.
1
Bromide
❑
❑
Concentration
Mass
(24959-67-9)
2.El
Chlorine, total
El
Concentration
Concentration
ppb
47
16.7
187
residual
3.
Color
✓❑
❑
Concentration
Mass
4.
Fecal coliform
0
❑
Concentration
Mass
5
Fluoride
ID
❑
Concentration
ppb
250
222.5
16
Mass
(16984-48-8)
6
Nitrate -nitrite
E
❑
Concentration
ppm
.23
0.15
16
Mass
7.
Nitrogen, total
❑
❑
Concentration
ppm
1 .58
.21
1 16
Mass
organic (as N)
8.
Oil and grease
❑
ConcentrationMass
Phosphorus (as
❑
Concentration
ppm
<D.1D
<0.10
16
Mass
9
P), total (7723-14-0)
10
Sulfate (as SO4)
0
❑
Concentration
Mass
(14808-79-8)
11.
Sulfide (as S)
0
❑
Concentration
Mass
EPA Form 3510-2C (Revised 3-19) Page 23

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCOO84387 Dukes VJTP 001 OMB No 2040-0004
Pollutant
Presence or Absence
check one
Units
(specify)
Effluent
Intake
(Optional)
Believed
Present
Believed
Absent
Maximum Daily
Discharge
(required)
Maximum
Monthly
Discharge
d available
Long -Term
Average Daily
Discharge
if available
Number of
Analyses
Long -Tenn
Average
Value
Number of
Analyses
12 Sulfite (as S03)
(1426545-3)
❑
Concentration
Mass
13. Surfactants
❑
❑
ConcentrationMass
14 Aluminum, total
(7429-90-5)
0
❑
Concentration
ppb
800
515
16
Mass
15 Barium total
V440-39-3)
El
❑
Concentration
Mass
Boron total
16. (744O42-8)
❑
❑
Concentration
Mass
17.
Cobalt total
(744048-4)
❑
❑
Concentration
Mass
18
Iron total
V439- H)
❑
❑
Concentration
Mass
19
Magnesium, total
(7439-95-4)
0
❑
Concentration
Mass
20.
Molybdenum,
total
439.98.7
❑
Concentration
Mass
21
Manganese, total
(7439-96-5)
0
❑
Concentration
Mass
22
Tin total
(74a0-31.5))
❑
❑
Concentration
Mass
23
Titanium, total
V440-32-6)
❑
❑
Concentration
Imass
EPA Form 3510-2C (Revised 3-19) Page 24

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP 001 OMB No.2040-0004
Presence or Absence
Intake
check one
Effluent
(Optional)
Pollutant
Believed Believed
Units
(specify)
Maximum Daily
Maximum
Monthly
Long -Tenn
Average Daily
Number of
Long -Term
Number of
Present Absent
Discharge
Discharge
Discharge
Analyses
Average
Analyses
(required)
if available
if available)Value
24.
Radioactivity
Alpha, total
❑
❑
Concentration
Mass
Beta, total
❑
❑✓
Concentration
Mass
Radium, total
❑
Concentration
Mass
Radium 226, total
❑
❑
Concentration
Mass
1 Sampling shall be conducted according to sufficiently sensifive test procedures (i.e., methods) approved under 40 CFR 136 for the analysis of pollutants or pollutant parameters or
required under 40 CFR chapter I, subchapter N or 0. See instructions and 40 CFR 122.21(e)(3).
EPA Form 3510-2C (Revised 3-19) Page 25

This page intentionally left blank.

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03I05119
100000062601 NC0084387 Dukes WTP 001 OMB No. 2040-0004
'••
Pollutant
7Present
rlAMsbestos
• 1
Presence or Absence
check one
Reason Pollutant Believed Present in Discharge
Available Quantitative Data
(specify units)
Believed
Believed
Absent
❑
✓❑
2.
Acetaldehyde
❑
❑�
3.
Allyl alcohol
❑
In
4.
Aly chloride
❑
❑�
5.
Amyl acetate
❑
❑✓
6.
Aniline
❑
❑�
7.
Benzonitrile
❑
❑�
8.
Benzyl chloride
❑
9.
Butyl acetate
❑
10.
Butylamine
❑
❑�
11.
Caplan
❑
12.
Carbaryl
❑
❑�
13.
Carbofuran
❑
14.
Carbon disulfide
❑
❑�
15.
Chlorpyrifos
❑
❑✓
16.
Coumaphos
❑
❑✓
17.
Cresol
❑
0
18.
Crotonaldehyde
❑
❑�
19.
Cyclohexane
❑
❑�
EPA Form 3510-2C (Revised 3-19) Page 27

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes VJTP 001 OMB No 2040-0004
Pollutant
r2,4D
Presence or Absence
check one
Reason Pollutant Believed Present in Discharge
Available Quantitative Data
(specify units)
Believed
Present
Believed
Absent
orophenoxyacetic acid)
❑
❑�
21.
Diazinon
❑
22.
Dicamba
❑
❑�
23.
Dichlobenil
❑
❑�
24.
Dichlone
❑
25.
2,2-dichloropropionic acid
❑
❑�
26.
Dichlorvos
❑
0
27.
Diethyl amine
❑
ED
28.
Dimethyl amine
❑
M
29.
Dintrobenzene
❑
30.
Diquat
❑
31.
Disulfoton
El
El
32.
Diuron
❑
❑✓
33.
Epichlorohydrin
❑❑
34.
Ethion
❑
❑✓
35.
Ethylene diamine
❑
❑�
36.
Ethylene dibromide
❑
❑�
37.
Formaldehyde
❑
El
38.
Furfural
❑
EPA Form 3510-2C (Revised 3-19) Page 28

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP 001 OMB No. 2040-0004
'1•
1 • 1Presence or Absence
check one
Believed Believed
Present Absent
Reason Pollutant Believed Present in Discharge
Available Quantitative Data
(specify units)
Pollutant
rGuthion
❑
❑✓
40.
Isoprene
❑
M
41.
Isopropanolamine
❑
El
42.
Kelthane
❑
43.
Kepone
❑
44.
Malathion
❑
45.
Mercaptodimethur
❑
❑
46.
Methoxychlor
❑
❑�
47.
Methyl mercaptan
❑
❑�
48.
Methyl methacrylate
❑
❑�
49.
Methyl parathion
❑
50.
Mevinphos
❑
51.
Mexacarbate
❑
❑�
52.
Monoethyl amine
❑
❑✓
53.
Monomethyl amine
❑
❑�
54.
Naled
❑
❑�
55.
Naphthenic acid
❑
❑�
56.
Nitrotoluene
❑
❑✓
57.
Parathion
❑
❑�
EPA Form 3510-2C (Revised 3.19) Page 29

EPA Identification Number NPIDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
10000G062601 NC0084387 Dukes WTP 001 OMB No. 2040-0004
1 • 1Presence or Absence
check one
Reason Pollutant Believed Present in Discharge
Available Quantitative Data
(specify units)
Pollutant
rPh.nolsulfortate
Believed
Present
Believed
Absent
❑
❑✓
59.
Phosgene
❑
0
60.
Propargite
❑
0
61.
Propylene oxide
❑
0
62.
Pyrethrins
❑
0
63.
Quinoline
❑
0
64.
Resorcinol
❑
65.
Strontium
❑
66.
Strychnine
❑
67.
Styrene
❑
0
68
2,4 5-T (2,4,5•trichlorophenoxyacetic
acid
❑
0
69.
TDE (tetrachlorodiphenyl ethane)
❑
0
70.
2,4,5-TP [2-(2,4,5-trichlorophenoxy)
ro anolc acid
❑
0
71.
Trichlorofon
❑
72.
Triethanolamine
❑
0
73.
Triethylamine
❑
0
74.
Trimethylamine
❑
0
75.
Uranium
❑
0
76.
Vanadium
❑
EPA Form 3510-2C (Revised 3-19) Page 30

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
100000062601 NCO084387 Dukes WTP 001 OMB No.2040-0004
Presence or Absence
check one
Believed Believed
Present Absent
Reason Pollutant Believed Present in Discharge
Available Quantitative Data
(specify units)
ollutant
rVmyI.c.*tte
❑
❑✓
78.
Xylene
❑
❑�
79.
Xylenol
❑
✓❑
80.
Zirconium
❑
❑�
Sampling shall be conducted according to sufficiently sensitive test procedures (i.e., methods) approved under 40 CFR 136 for the analysis of pollutants or pollutant parameters or
required under 40 CFR chapter I, subchapter N or 0. See instructions and 40 CFR 122.21(e)(3).
EPA Form 3510-2C (Revised 3-19) Page 31

This page intentionally left blank.
RECEIVED
SEP 0 4 2024
NCDEQ/DWR/NPDES

EPA identification Number NPDES Permit Number Facility Name Oulfall Number Form Approved 03/05/19
100000062601 NC0084387 Dukes WTP 001 OMB No. 2040-00M
Presence or
TCDD
Congeners
Absence
Pollutant
check one Results of Screening Procedure
Used or
Believed Believed
Manufactured
Present Absent
2,3,7,8-TCDD
E]
El
IZI
EPA Form 3510-2C (Revised 3-19) Page 33

Attachment A
Section 1: Outfall Location Map

DocuSign Envelope ID: 99CAD692-D859-4A89-B86E-F54550E08CEB
Lake Norman
1�
McDowell Creek,\t�
[flows south] l.700�C \1 1 . 111 11
JSO
Babe Stillwell Farm Rd \ t� Outfa11001
),
Approximate
[flows south] /
� . � -! � � J
Facility Boundary
^,�O
•ii'�}►
Charlotte Water
Lee S. Dukes Water Treatment Plant (WTP)
7980 Babe Stillwell Farm Road, Hunterville 28078
Receivinz Stream: McDowell Creek Stream Class: WS-IV
Stream Se¢ment: 11-1 I5-(I.5) Sub -Basin: 03-08-33
Drainaee Basin: Catawba HUC: 030501011401
Latitude: 35° 25' 42" Longitude: 81 ° 53' 17"
State Grid/USGS Quad: Lake Norman S
64)
Facility
Location
'NCO084387
lI,
111lenburg

Attachment B
Section 2: Line Drawing

Lee S. Dukes Water Treatment Plant Process Flow Diagram as of 29 August 2024
3ECEIVED
y�
CIO, Alum Polymer,
A
L -.
1
M
Carbon Chlorine KMn04
1
a t
t
From v
Lake 10
Raw Water Avg: 19.0 MGD
Norman Alex. Design: 25.3 MGD
Rapid I I Flocculation
Mix Avg: .6Basin 1
MGD
Mex. Design: 12.65 MGD 5968 G 208639 G
I Rapid I Flocculation
Mix 2 Basin 2
Avg: 9.50 MGD 5968 MG 208639 G
Max. Design: 12.65 MGD
LEGEND
Process Colors
Symbols
Raw Water
Finished I Potable Water
cnem Chemical Feed
Name
a>•� Sludge
##
Backwash Waste
/�PPum�p
High Service Pump
Settled /Clarified Water
\J
Chemical Colors
Process Flow Information
Potassium Permanganate (
KMnO, )
Avg: 9.50MGD Annual Daily Average Flow Volume
Chlorine
Max. Design: 25.3 MGD Maximum Design Flow Rate
Alum
MGD Million Gallons Per Day
Power Activated Carbon ( PAC )
MG Million Gallons
Sodium Bisulrite
G Gallons
jW __s Polymer
Chlorine Dioxide ( C102 )
®® Fluoride
Sedimentation
Basin 1
1.5MG
Sedimentation
Basin 2
1.5MG
Filtered Water
Avg: 4.675 MGD Avg: 4.6 MGD
Filter 1
Chlorine 89,648 G
Backwash Waste
_ Settled Water
Avg: 9.35 MGD Avg.' 4.675 MGD Avg: 4.6 MGD
Filter 2
89,548 G
settlad water
Avg: 9.35 MGD Avg: 4.675 MGD
Avg: 4.675 MGD
Filter 3 I Avg. 4.6 MGD
89,648 G
Avg. 4.6 MGD
Filter 4
89,648 G
Chlorine Lime
1\J1rDE /DWR/NPDES
---------
High Service
Fluoride
I Pump Station
Pump 1
I I
IIF Clearwell
I I
Finished Water
Filtered Water Avg. 6.135MGD 91
MG
I Pump2 I
I
I I
I I
I
I Pump
I
Clearwell
I I
I Wet I To
2
I Well Pump 10 978
Avg: 6. 135 MGD
9 MG
I 89,648 G I Zone
I I
IF
I
I Pump 5
I I
Clearwell
I
I
Avg: 6.135MGD 9 MG
I
i Pump 6
I
I I
I I
I I
L— — — — — — — — — — — — — — —
Sodium
Bisulfite
Backwash
ste
Waste EQ Basin Clarifier
Avg: 0.294 MGD 509,085 G Avg 0.554 MGD 509,085 G Avg0 554 MGD
Max. Design: 13.8 MGD Max. Design 2.0 MGD (I
C a Solids
oy
ca
i6on Basin Blowdown Sludge Thickener
Estimated: 0.3 MGD 176,154 G Intermittent
S30dN/8M®/0300N
to
-b-FAI
1 �I
`•
Dechlorination To Creek
Contact Non -Potable We OUtfaIl
Chamber Avg:0.554MGD
12700 G i 001
Residuals
Lagoon
670000 G
Residuals for Land Application

Attachment B
Treatment Process Synopsis
Water Treatment Process
Raw water supplied to the Dukes plant is treated with potassium permanganate and powdered
activated carbon as needed. Aluminum sulfate is added at the head works of the plant. The
water then passes through three stages of flocculation before entering the sedimentation
basins. Chlorine, to begin disinfection, is added just before filtration. Following filtration, lime,
fluoride and chlorine are added to the filtered water prior to flowing to clearwells for onsite
storage. In the future, there are plans to apply chlorine dioxide in place of potassium
permanganate into the raw water of Lake Norman before entering the treatment process,
however, no implementation date has been set and construction has not begun. Ferrous sulfate
may be applied in the future as well in conjunction with chlorine dioxide. Since ferrous sulfate is
not currently being utilized in the treatment process, we respectfully ask that quarterly Total
Iron monitoring be removed from the permit sampling requirements.
Filter Backwash, Residuals and Sludge Management Process
Filter backwash water is discharged to an EQ basin. The EQ basin pumps water to a clarifier.
Solids are generated from the water treatment process, consisting of alum residuals. The solids
that settle on the bottom of the clarifier are discharged to a thickener before being directed to a
holding lagoon. Solids generated from the sedimentation basins utilized in the water treatment
process are also discharged to a gravity thickener. From the bottom of the thickener solids are
sent to a holding lagoon. Solids from the holding lagoon are removed by a contractor
(Synagro) and land applied according to permit requirements (WQ 0000057). We also have the
ability to tanker the liquid residuals to a belt press prior to land application or land fill disposal.
Clear water from the top surface of the clarifier flows over a weir and into a de -chlorination
chamber where sodium bisulfite is added before being directed to the NPDES discharge point
(Outfall 1). Supernatant from the lagoon is intermittently discharged to the receiving stream.

Attachment C
Section 7: Waiver Justification

CHARLOTTE
Wt)TER
August 30, 2024
ATTN: Mr. Joe Corporon
Division of Water Resources
Environmental Specialist 1
Water Quality Permitting Section — NPDES
1617 Mail Service Center
Raleigh NC 27699-1617
Subject: NPDES Permit Renewal Application
Lee S. Dukes Water Treatment Plant - NPDES Permit # NCO084387
Waiver Request for Table A Pollutants
Mr. Corporon,
Charlotte Water respectfully requests a waiver for monitoring several of the parameters
included in the Table A list of contaminants. COD, BOD and TOC have never been constituents
required to be monitored at this location. The current permit requires weekly monitoring for
Flow, Total Suspended Solids, pH, Total Residual Chlorine and Turbidity. Required quarterly
monitoring includes the following parameters: Aluminum, Fluoride, Phosphorus, Total Kjeldahl
Nitrogen, Nitrate, Nitrite, Total Nitrogen and Chronic WET Testing for Ceriodaphnia dubia.
Lee S Duke Water Treatment Plant is categorized as a conventional technology water treatment
plant [utilizing coagulation, flocculation, sedimentation, and filtration] with discharge of
wastewaters from media filter backwash and sedimentation basins to freshwater. Considering
the type of wastewaters produced, Charlotte Water does not see the justification in being
required to add COD, BOD and TOC as additional monitoring parameters, given the excellence
of the effluent water quality leaving the facility as validated by the empirical data required by
the current NPDES permit. Please consider granting Charlotte Water a waiver for these
parameters.
If you have any questions, please contact Dave Banick at 704-634-9104 or Gabe Sasser at 704-
507-5267.
Sincerely
V, .o."
Gabe Sasser
Operations Manager
Charlotte Water
Charlotte Water 5200 Brookshire Blvd, Charlotte, NC 28216 charlottewater_org
46 Operated by the City of Charlotte

RECEIVED
SEP 04 L"024
NCDEQ/DWR/NPDES
Attachment D
Section 9: Biological Toxicity Tests

Ec nv�ronmcnta Inc. fC641 B77{':Y2 . rn), JX-410 7 WM
PO. Sox 1c414. Greenvale ;C 2J606 :: CWflarttdtl CUJF.. Gr_Fr. SC 2965-Y.:
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 13.Jun-21
Laboratory ID #: T58277
Test Reviewed and Approved By:
Robert W. Kelley, Ph.D.
QA/QC Officer
Cerdticatlon#E87819 SCDHEC Certification#23104
Test results presented in this report conform to all requirements of
NELAC, conducted under NEIAC Certification Number E87819
Florida Dept. of Health. Included results pertain only to provided sarrtples. NCDENR CerdIlcatlon # 022
Page 1 of 6

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 26-Jan-21
Facility: CHARLOTTE WATER Lee S. Dukes NPOES# NCO084367 Pipe # 001 county-, Mecklenburg
Laboratory Performing Test: •
Cormtents
X
Signature of Operator In Responsible Charge
X
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Envlronment2lSciences Branch
Div. of Water Quality
N.C. DENR
1621 Mall Service Center
Raleigh, North Carolina 27699-1621
North Carolina Cerlodalahnla Chronic Pass/Fall Renr2duGligoQjSIcItyTest Chronic Test Results
Calculated t= 0.5058
Critical Value= 2.508
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Reduction= 1.9%
# Young Produced
Adult (L)ive (D)ead
Effluent %
TREATMENT 2 ORGANISMS
# Young Produced
Adult (LNve (D)ead
pH let sarroe
Control
Treatment 2
start end
D.O. 1st e
Control 8.1 7.6
Treatment 2 8.3 7.9
LC501Acute Toxicity Teat
(Mortality expressed as %, combining replicates)
23 22 1 22 1 20 1 22 1 22 1 20 1 26 1 21 1 23 1 20 1 24 % Mortality Avg. Reprod-
L L I L IL IL-
L IL I L I L I L I L L 0% 22.1
Coned Control
0% 21.7
Treatment 2 Treatment 2
Control CV
1 2 3 4 5 6 7 8 9 10 11 12 8.1%
21 22 22 22 24 20 iB 17 25 24 22 22 % 3rd Brood PASS FAIL
L L L L L L L L L L L L 100% X
FSwnle
This for EHMr Tsst Test Start Date
t3-Jan-21
1st sample 2nd sample 12-fen-21 Sarrtole 2 14-Jan-21
7.8 8.2
7.7 8.4
start and start end
15t a 2nd e
7.8 8.9
8.7 8.6
Sample 1 1 x
Sample 2 k
Hardness (mg/L)
Sp-. cord. (vrrt—)
CN— (mg/L)
at
1st 2nd
Tox Tox
Dilution Sample Sample
81.6
313 56 56
1<.05 <.OS
0.6 0.4
Natality starUend start/end
-1 LLC50 = % Method of Determination con"
95% Confidence Urnlls 'ng Average Pfobit High Conc.
% pearmen Kerber her pH D.O.
O anism Tested Cerioda hnia dubia
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facility: CHARLOTTE WATER
NPOEsu NC0084387
Sample ID: Lee S. Dukes
Errs rsazrr
Date: 13-Jan-21
borato :
Certification #: NCO22
F .Date: 11/I/2021
Survival Data
7 Day Survival Test Used: FISHERS TEST
Control 100% Test Statistic: P= 1.000
Effluent 100% Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test for Normality
Mean young/female
Sid. Dev,
Test Used: Shapiro-Wilks Test:
Control 22.1
1.78
Effluent 21.7
2.23
. W:
0.971
Critical Value:
0.884
Analysisfor Differences in Reproduction
Test far Homogeneity of Variance
Test Used: Equal Variance t Test
Test Used: F Test
Calculated t=
0.51
F=
1.57
Critical Value=
2.51
Critical Value=
5.32
The data are homogeneous In variance
PASS: The effluent is not chronically toxic.
Page 3 of 6

Day
•ay
7 a1
58277
CHARLOTTE WAT
it 10
Lea S. Dukes
DEW
NCO084387
ordh
MecOenburq
1
i Il/d oals
13-Jan-21
A hd Tlma
1:00 PM
god
ic
aalCeciodapfinia&6a
bam dNW
12Jan-21
barn One
1715-2200
pt
NCCPF
Vhftr
MHSF 116W D
for Cone.
%
7
BROOD
aN 1rMeaill
30 ml
edvoiusa
15 ml
i
1
16WBdk
T 'C
24.8
0.05 ml
AT
05 ml
Wmelwd
as en-a-0z-oiz:ouz
Page 4 of 6

ETT
PO Brno 16414, Greettvlae, SC 41W8-7414
(BB4) 877-6942, (BW) 891-2325 Fa)c(864) BT7 69M
Shipping Address 4 Craftsman Ct, Greer, SC 29650
-/�^/WRTeJtV lqO NM LiTA{..C�N
CHAIN OF CUSTODY RECORD
Pa--e —/ of
Client: C+�Q D Q W&e
t
Program
Containers
Preservative
Parameters
Facility: �,� �kiCtS Wi'P
Whole Eftlaw Tozid
_
p
State N NPDES r N C M 0'i 4 3
Acu1
jCbm.1c
I Test Qrgaaisms
"
St
0
n
V(COmpasitn
only)
((hob Or Compaslte)
¢
O
V
p
Ic
L=
F
z
Sign, and Print below
3
>
=
u
a
-JfCL
L
U
-
c
—
-
_
,,,
>
v
Sf' I+E ID
0
U
the dotted line
e
U
=
O
c
C
v
W
.�
t'f-ti]oti
c
3-two;
°
.-
.-
_
a
n
s
�'
Cmnp�nt� Stet Dme 71me
S.,„pte Colleetion D�Ic 71me
Collected by
U
y
co
z
a
C7
]
a-oma
¢
Q
'U
U
Ci
a
L
i
v'1
_
U=
3
2
Chemical Anal ysla80ther
a
am
m
t-12-z�
=Tt�►,r, vn� E�
L
Il K
I
'AL
)c
mil.
x
-V
�827�A
--------------
Special Instructions:
Sample Custody Transfer Record
Secure
Receipt
Samplc
Date Time Relinquished B I Organization Received B / Or¢ tion
1'12'2r OW3 i'r5
Area
Temp 'C
Preserved?
�iv�-�'ia►-�o{'�C wti�r
CObIPOSITESAMPLING PROCEDURES
TE4PERdTURE MONITORING PROCEDURES
HOLD TIME PROCED URLS
Composite sunples must be collected over a 24 hour period.
Time Proportional: 7 sample each. hour for 24 hours. Equal volw
Sample temperattuz during collection and transport niust be between
0.0 6.0 *C.
For toxicigl testing the sample must fi,-si be used lvithin 36 hours
or at minimum 1 sample every 4 hours over 24 hours.
and Samples must not be fmzen. Use water ice in sealed bass.
of sample collection (completion of composite sample).
Flow Pro ortional: As per instructions in NPDES permit.
Sample may not be used after 72 hours from sample collection.

ETT
PO Bax 1 n414. Gresmrll4, SC 7.DB08-7414
[phone], [phone] Fax(884) ST7 8938
Shipping Addmu: 4 Crsrnaman Cl. Greer, SC 29650
w"/'./.CrTCN VI RONMCNTALe ON
CHAIN OF CUSTODY RECORD
Paae o=
Client:
Char Ig
He .L+
Program
Containcrs
Prucn•atitc
Paramctcrs
Facility:
Lee S. e P
XrWe em—t ro:ior,
State: L NPDLS #` N 2114
Acucc
lChrunic
I Test Organisms
—
<
—
U
e
C
U(Canpmitemly)
(Grab or Composite)
<
o
o`
_c
U
c
U
o
Z
Sign, and Print below
e
m
i. Nc a
_
=
on
=
E
•,
<
the dotted line
a
Q4
U
`
.2
H
y
c
a-HNoa
I-NaOH
m
p
_
SAMPLE ID
v
Conpd�e 5t.n Dole Time
Sweplc Calieevw D.�c Time
Colleca eJ by
°'
U
8
to
3
h
a
ZZ
o=
x
r—
O
o
>
—
s=Znac
a. p
s
¢
n
<
L_-
U
U
U
0
t-.
GT
✓7
T
U
!-
Chemical Analyals 6. Other
-----------
-------------
-------------
Special Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Relinquished B / Orpyanization Received BY / rs�nization
Area
Temp °C
Presem ed?
I- w• al
/4h f
1
l
% ��/ �,.�
, .
Zv
�zr'
COMPOSITE SAMPLING PROCEDURES
TEMPERATURE MON/TORING PROCEDURES
HOLD T1.1IE PROCEDURES
Composite samples must be collected aver a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used Mthin 36 hours
Time Proportional: I sample each hour for 24 hoius. Equal volw
0.0 and 6.0 °C_ Samples must not be frozen. Use eater ice in sealed begs.
of sample collection (completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours.
Sample may not be used afrer'P_ hours from sample Collection.
Flow Proportional: As per instructions in NPDES pemiit

ETaniilne. f1(i-ti`i77,.. 1•-�i.1i.,,
PO Box IE-414 Greenvlle SC 29606
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 14-Apr-21
Laboratory ID #: T58980
Test Reviensed and Approved By:
Robert W, Kelley, Ph.D,
QA/QC Officer
AIR
k
Certification #E87819
Test resuhs presented in this report condor to all requirements or
NELAC. conducted under NELAC Ccnificaiion Ntnnbcr E87819
Florida Dept. of Health. Included resudta pertain only to provided wm les.
age 1 of 6
SCDHEC Certification #23104
NCDEAR Ccrtificatlon # 022

Effluent Toxicity Report Form - Chronic Pass/Fall and Acute LC50
Date 28-Apr-21
Facility: CHARLOTTE WATER Lee S. Dukes NPOESa NCO084387 Pipe # 001 County. Mecklenburg
Laboratory Performing Test.
.
Comments
X 1
Signature of Operator in Responsible Charge
X
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 2T699-1621
North Carolina Ceriodaphnla Chronic Pass/Fail Reproduction Toxlclty Test Chronic Test Results
Calculated t= 0.2938
Critical Value= 2.508
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 %Reduction= 1.1°/
t4 Ycung Produced 24 24 24 20 24 23 26 23 21 21 24 21
Adult (LOve (Dread L L L L L L L L L L L L
Effluent %
TREATMENT 2 ORGANISMS
M Young Produced
Adull Ove (D)ead
1 3 4 A A 7 R 0 1n 11 19
21
24
24
26
22 1
24
25
19
19
22
25
21
L
L
L
L
L
L
L
L
L
L
L
L
pH 1st sample 1st sample 2nd sample
Control 7 6 8. t 7.8 8 0 78 8.1
Treatment 2 7 8 8.0 7.8 7.9 7.8 80
start end start and start end
D.O. 1st a 1st sample 2rd sa e
Control 7.8 8.2 8.2 7.8 7.9 7.9
Treatment 2 8.2 8.5 8 6 7.9 8.1 7,9
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicales)
LC50 = %
95% Conkdence Limits
Oraanism Tested
to This for Either Test
M (Start) Date
1 13-Apr-21
Sar ple 1
Sample 2 1 \
Hardness (mg/L)
Spec. Cond (pmhos)
Chlorine (rrg/L)
rote Tempat receipt ('CI
0%
Control
22.9
Coned
0%
realmartt 2
22.7
Treatment 2
mlyd CV
7.8%
PASS FAIL
% 3rd Brood
100%
Test Start Data
14-Apr-21
Sample 2 15-Apr-21
161 2rd
To■ Tax
Dilution Sample Sample
87.2
321 64 67
<.05 <.05
0.5 0.9
Moruvay slarvend startlend
Method of Determination Islas= contra L- - I i
Average R01her
Probl1 � High Corcan Karber pH D.O.
dubia
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facility: CHARLOTTE WATER
NPDESa NC0084387
Sample ID: Lee S. Dukes
ETTM 158980 Oate: 11-Apr-_ I
Laboratory:
Certification::: NCO22
FIIP Dale: I I!li'_021
Survival Data
7 Day Survival Test Used: FISHERS TEST
Control 100% Test Statistic: P= 1.000
Effluent 100% Critical Value: P= 0.01
PASS: The effluent does not reduce sun-ival of the test organisms.
Reproduction Data
Raw Data
Tesrfor Normality
Mean young/female
Sid. Dev.
Test Used: Shapiro-Wilks Test:
Control
22.9 1.78
Effluent
22.7 2.35
W:
0.942
Critical Value:
0.894
Analysis for Differences in Reproduclion
Tesrfor Homogeneity of Variance
Test Used: Equal Variance i Test.
Test Used: F Test
Calculated t=
0.29
F=
1.74
Critical Value=
2,51
Critical Value=
5.32
The data are homogeneous in variance
PASS: The effluent is not chronically toxic.
Page 3 of 6

Control Mortality and Reproduction by Test Day
!Q'!
R9 4-1
684-2
U7 4-2
N6 1-1
F7 4-2
37 % Effluent Mortality and Reproduction by Test Day
764-2
0--__-_-
---___-
--_____
Comments
AND 58981 SHARE A CONTROL
Page 4 of 6

ETT
A Tn6T
��
PO Box 15414, Greenville, SC 29606-7414
[phone]• [phone] Fa)c(864) 877 6938
Shipping Address: 4 Cransman Cl, Greer. SC 29650
WWW-CT NVWDNN[NTALCCM
CHAIN OF CUSTODY RECORD
Page
Client:
Chai--lo4f�,- wFtz,
Program
Containers
Presen•ative
Parameters
Facility: j S �ti k t 5 Ov TV
—
lVholc Lllucnt Toxirirc
n
State: IVL NPDLS n: NC CfJ0
Acute
Chronic I
Test Organisms
s
u
s
U
(Composite only)
(Grab or Composite)
<
=
U
i
o
Z
_
=
-
Sian, and Print below
5
c
=
o
2m HCL
N05
SAMPLE ID
U
V
Compmee s nn Oste Time
sample Conmfan nme Time
the doned line
Collected by
c
U
E
v
E
•—
v1
C
—
z
L+
•°
—
�
t7
=
=
>
�
-`
4�Ra0H
6. p o
UU
<
<
-
U
-
J
=
U
C
—
�
L
.l
=
U
=
3
c
Chemical Analysis & O[her
001
G
�(-t'3 - z
- m M': C zr
X
1.6�L
x
'JI
Ili
0
-------------
I
I
I
Ill
IIII
Specinl Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Rclin uis}tEil $v /Or? anization Received BY rganizalion
-Vrez
Temp =C
Presen ed?
LO-3 -7-1
P, L
i• r
0.
COMPOSITE -%4 HPL ING PROCEDURES T&UPERNTURL IfONITORJAIG PROCEDURES HOLD TIAL PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be bctwcen For tosiciry resting die sample muse first be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal voltn 0.0 and 6.0 `C. Samples most not be frozen. Use hater ice in scaled baps. of sample collection (completion of composite sample).
or at minimum t sample even 4 hours over 24 hours_ Sample may not be used after 72 hours `f om sax-riple collection.
now Proportional: As per instructions in �FPDES pemtii

ETT
PO 9au 115414, Gnmrrvilte• SC 2e606-7414
(864) BT7.8942, [phone] Fatc(884) 87 8938
Shipping Address: 4 Cranstnan Cl, Greer, SC 29650
www.rrrctivrw..+.. Irwr .t:or.
CHAIN OF CUSTODY RECORD
Page L_ 0f7
Client:
Program
Containers
Preson•ative
Parameters
Facility: Lciz-
"'hole Cmacnt Toiidh
State' M C— NPDES /ft/
Acoce
Chronic
Tat 0 eolsms
U
(Composite ortiv)
(Grab or Composite)
Z
o
�
=
LE
c
L
U
o
.,
J
Z
i
Sign, and Print below
3
=
m
_�
-
-HCL
—
the doned line
=
E
u
V
H
u
.-woH
o
o
y
=
2
a
—
SAMPLE )D
o
cs asun oue Time
mpdt
s.mpje CQnactbM One Time
Collected br
"
U
h
u
N
o
Z
a
G
U
>
• znac
6. o'k,
u
<
u`-—
<
U
V
U
L
h
—
(J
=
3
Chemical Analysis G Clther
_,01 '
OVA
0
-------------
I)
a)
-------------
Special Instructions:
I
Sample Custody Transfer Record
Secure
Receipt Sample
Date Time Relinquished B ! Organization R v !Organ' lien %
area
Tem eC i Preserved?
Lj. is •-X
„9 C r l o y e Vvcc t cr
Z G C�
/Lf/o
COAdPOSITE SAA-fPLING PROCEDURES TEA•IPEJUTURE MONITORING PROCEDURES HOLD TlA{£ PROCEDURES
Composite simples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toXicit\' testing die sample must fim be used "Aidiin 36 hours
Time Proportional: i sample each Hour for 24 hours. Equal volut 0.0 and 6.0 *C. Samples must not be fTozen. Use water ice in scaled bass. of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 Hours. Sample may not be used after 72 hour from sample collection.
Flow Proportional: As per instructions in NPDES permit.

EC nviranncrita Inc
PO Box 1E414 Greenvale SC 29606
u clanT1f.111 Cf."a • t St . ram'
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facilih•: Lee S. Dukes
NPDES #: NCO084387
Test Date: 14-Jul-21
Laboratory ID #: T59621
Test Reclened and Approved By:
441- 4/.
Robert W. Kelley. Ph.D,
President
6 _
Certification #E87819
Test results presented in this report confomt to all requirements of
NELAC. conducted under NELAC Certification Ntanber E87819
Florida Dept. of Health. Included results pertain only to provided Its.
,MTage 1 of 6
Patrick D. Timms
QA/QC Officer
SCDHEC Certification #23104
NCDENR Certification # 022

Effluent Toxicity Report Form - Chronic Pass/Fall and Acute LC50
Date 26-Jul-21
Facility: CHARLOTTE WATER Lee S. Dukes NPDESN NCO084387 Pipe N 001 County Mecklenburg
Laboratory Performing Test:
Comments
X t
Signature of Operator in Responsible Charge
X ,6 4
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mall Service Center
Raleigh, North Carolina 27699-1621
CONTROL ORGANISMS
N Young Produced
Adult (LNve (D)aad
Effluent %
TREATMENT 2 ORGANISMS
N Young Produced
Adult (Lr°e (D)ead
t 2
Calculated 1=
Critical Value=
4 5 6 7 8 9 10 11 12 % Reduction=
-0,1099
2.508
0% 20.8
Control Control
0% 20.8
reatmenl 2 Treatment 2
Control Cv
1 2 3 4 5 6 7 8 9 10 11 12 9.7%
20
22
20
22
16
23
21
2t
2d
19
"1(1
20
L
L
L
L
L
L
L
L
L
L
L
L
7, 3rd Brood PASS FAIL
100% X
late This for Eithw Test Teat Steil Date
14-Jul.21
F2 15-Ju021
nr7 1st sample 1st Samoa 2nd sample Sample 1 13-Jmi- 1 Sample 2 -
Control 7.8 8.3 7.8 8.1 7.8 8.1
Treatment 2 8.3 8.2 8.0 8.1 7.9 7.9
start end start and start and
D.O. 1s1 sample ts1 sa a 2nd e
Control 8.1 8.3
Treatment 2 83 8.4
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
LC50 = %
95% Confidence LIml9
Oraanlsm Tested
Method of Determination
Average Probil
an Karber FOther
CeriodaQhnia dubia
Sarnrwe i x
Sample 2 X
Hardness (rmWL)
Spec Cond (pmhos)
Chlorine (mg7L)
mole Temoat recemoi CC)
1st 2nd
To: To.
Dilution Sample Sanple
85.4
321 74 73
<0.05 <0.05
3,0 35
Mortality slant end slanfend
Control
Hph Cons
pH D O.
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facility: CHARLOTTE WATER
NPDESa NC0084387
Sample ID: Lee S. Dukes
Err Ts%21
Date: 14-Jul-21
Laboratory,
Certification #: NCO22
Exp. Date: I I/l/2021
Survival Data
7 Day Survival
Test Used: FISHERS TEST
Control 100%
Test Statistic: P= 1,000
Effluent 100%
Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Ra,,, Data
Test jar Normality
Mean young/female
Std. Dev.
Test Used: Shapiro-Wilks Test:
Control
20.8 2.01
Effluent
20.8 1.70
W:
0.462
Critical Value:
0.884
Analysisfor Differences in Reproduefiun
Test for Homogeneity of Variance
Test Used: Equal 1'ariance
I Test-
Test Used: F Test
Calculated t=
-0.I 1
F=
1.40
Critical Value
2.51
Critical Value-
5.32
1-lie data are homogeneous in variance
PASS: The effluent is not chronically toxic.
Page 3 of 6

Control Mortality and Reproduction by Test .,
3 4 5 6 7 8L5
lQ�!2
7-1
7-2
_--_®-�-
A2 7-2
----�-®-
_---�_�_
115 7-7
37 % Effluent Mortality and Reproduction by Test Day
t
t
t--_---_
IYi:Y
t
59621
Hunt
HARLOTTE WAT
kr ID
as S. Dukes
PDES#
NCO084387
ou
acklenourg
onth
tut S red DOW
14-Jul-21
tut ti fed Time
2:11 PM
rtsd a fed B
ssl fun
so. born daft
. born tlms
iz
Coriodaphnia dubia
13-JJ-21
1700-2200
out
NCCPF
lutlon Water
nits for Conc.
C
MHSF
%
37
Snd BROOD
set vessels
30 ml
sal volume
bslor 11
t
ntW) Tom 'C
15 all
1
161UBdk
2C8
tnun
0.05 mi
AT
out method lk,..2..R-0:-o13
05 ml
IJ03
Comments
1,59622.59624 BLOCKED
N/A -
Page 4 of 6

ETT
PO Box 16414, Greerrville, SC 29608-7414
[phone], [phone] Fax:(864) 8—/7 6936
Shipping Address: 4 Craftsman C1. Greer, SC 29650
-.W. CTTCN V Iq9 N, CNTAL.00m
CHAIN OF CUSTODY RECORD
Client:
Char i
Program
Containers
PTUem'alive
Parameters
Facility:
wbote rmucnt Tuxichy
—
/
State: NPDES m: ji pp��
vtJ ^�
Acute
CL•roni[
lest Ortlnnisrtis
CJ
(Composite only)
(Grab or Composite)
i
i
_
J
—
Sign, and Print below
5
=
=
c
=
u
•HCL
iiz
`—.
the dotted line
C
U
u
;-livo3
=
z
u
u
SAMPLC ID
U
C—paui, Sun Dne Time
s—pte Conccti- One Time
Collecte l by
U
)
u
5�2n4c
v=ouK
u
<
o
<
t
Uv
=
U
L
Chemical P,n aiysis 3 Other
oo
r
x
rt
V,d?�a----
-------------
°'
- - - - - - - - - - - - -
- - - - - - - - - - - - -
S pcciol Instructions:
Sample Custody Transfer Record
Secure
Receipt
I
Sample
Date Time Relin uislted By lOrsanizaton eceived B\ Oreanization
Area
Tcmo'C
Presencd?
c r c 1.✓- e''I
t u T l
3 (
11:,7D
t
1 !
3ra
I
COMPOSITE SAMPLING PROCEDURES
TEMPE24TURE A/0NlTOR1A'G PROCEDURES
HOLD ThVE PROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must fire be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal voles
0.0 and 6.0 T. Samples must not be frozen. Use water ice in sealed bags.
of sample collecdon (completion of composite samp)e).
or at minimum I sample ever, 4 hours over 24 hours.
Sample may nor be used after 72) hour fmn1 san rile collecden.
Flow Proportional: As pe.r instructions in NPDES permit.

ETT
PO box 18414, Greanvaie, SC 2960i 7414
[phone], [phone] rex_(864) 877 e938
Shipping Addtess� 4 Craftsman Ct, Greer, SC 29W
Www. CTTCNvi Of]rvw C,vTti.COr+
CHAIN OF CUSTODY RECORD
k.ncncLk 0(tom
`�t!
G
Progr9m
Conminers
Preservative
P1r'AmcTCrS
Facility: �� � r
<
3
U
-
=
o
s
Z
`u
=
Z
_
U
>
_-HCL
6-00-
whot. Ent eant Tcciciq
y
J
—
—
G
CnQmieQi Anil}'SIs a Other
2
State: /VC- NiPDE.S NC CO
Acute
Chronic
Tat Oreanii—
SAMPLE ID
0
U
(Compasneonly)
Compmm5 nn Dam Time
(GrabarCorMocrte)
SampleConmlon Detc Time
Sign, and Prior below
the dotted line
Cnllencd by
-
<
U
<
U
U
_
U
_
U
O
c
=
=
_
2
_
>
q
—
,
-
=
=
j
owl
-10
�-��-z�
�--<�.--3c,----
1.
f•`��
k
"/
x
x
�
�
�
+
5R(�aIP�
-------------
III
IT
-------------
11
MIT
Special Instructions:
Sample Custody Transfer Record
Date Time Relinquished By / Organiza ' Received By ! Organization
, l
Secure
Area
Recelpi
I TemD'C
sample
Preserved'
rr-
S
i
COMPOSITE SAMPLING PROCEDURES
Composite samples must be collected over a 24 hour period.
Time Proportional: I sample each hour for 24 hours. Equal volui
or at minimum I sample every 4 hours over 24 hours.
Flow Proportional: As per instructions in NIPDES permit.
TEMPER4TURE d/OMITORINC PROCEDURES
Sample temperature during collection and transpon must be between
0.0 and 6.0 *C. Samples must not be frozen. Use water ice in scaled bass.
HOLD TI.if£ PROCEDURES
For toxicity testing die sample must first be used within 36 hours
of sample collection (completion of composite sample).
Sample may not be used after 71 hours from sample collection.

cnvironI nontal Inc. G-4 `i 17 r• 4I tj
1 t AY ctji. +'
r 0 Dox 104 14 Greenvlie SC 20606
C,.flir n.fn Cr.xu Gt .+;r SG �)r3'�=
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1.002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 13-Oct-21
Laboratory M #: T60360
Test Reviewed and Approved By:
441 4/ ecru
Robert W. Kelley. Ph.D.
p� President
s
i
Certification #E87819
Test results presented in this report conform to all requirements or
NELAC. conducted under NELAC Ccri fication Number E,97819
Florida Dept. ol'Ilaullh. Included results pertain only to provided samples
gage 1 of 6
Patrick D. Timms
QArQC Officer
SCDiiEC Certification#23104
`CDENR Certiricatlon# 022

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 29-Oct-21
Facility: CHARLOTTE WATER Lee S Dukes NPDESN NCO084387 Pipe It 001 County: Mecklenburg
Laboratory Performing Test:
Comments
X t ' -
Signature of Operator In Responsible Charge
X
Signature of Laboratory Supervisor
MAIL ORIGINAL TO
CONTROL ORGANISMS
p Young Produced
Adult (L)Ive (D)ead
Effluent %
TREATMENT 2 ORGANISMS
M Young Produced
Adult (L)ive (D)ead
pH
Control
Treatment 2
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mall Service Center
Raleigh, North Carolina 27699-1621
Chronic Test Results
Calculated t=
Critical Value=
2 3 4 5 6 7 8 9 10 11 12 % Reduction=
22
20
23
24
22
19
19
18
18
21
22
23
L
L
L
L
L
L
L
L
L
L
L
L
1 2 3 4 5 6 7 8 9 10 11 12
213 19 23 1 18 20 23 23 23 22 19 22 19
L L L L L L L L L L L L
Complete Tlds for Either Test
Collection (Start) Date
1st sample 1st sample 2nd sample Sample 1 12-Oa-21
7.6 7.8 7.7 7.6 7.7 8.3 5emcda?ym l2u ar Font
7.5 7.8 7.5 7.8 7.6 8.2 Grab Camp Dural
Vail end start and start end
D.O. 1st samOle 15l sa le 2nd sa e
Control
Treatment 2
LC50/Acute Toxiclty Test
(Mortahly expressed as %. combining replicates)
LC50 = %
95N Conf-dence Limits
Organism Tested
,ample 2 lt�
Hardness (mg&)
Spec. Cond (Wmhos)
Chlorine (mgfL)
1pld Temp. at receipt ('C)
0
2.508
0.0%
0%
20.9
Control
Control
0%
20.9
Treatment 2
Treatment 2
Control CV
% 3rd Brood I PASS FAIL
100%
Test Start Dale
13-0d-21
Sample 2 14-OL1-21
Is( 2nd
To. lox
Dilution Sample SanVIe
87 5
316 74 73
< 05 <.05
0.8F 2.9
Mortal °y slarVand startfond
Method of Determination Can"
Averaoe Problt I High Corn.
an Karber ROther pH D-O
dubia
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facility: CHARLOTTE WATER
nroEsA NCO094397
Sample ID: Lee S. Dukes
ETTO T60360
Date: 13-Oct-21
Laboratory-,
Cenification #: NCO22
I Exp. Date: I I/1/2022
Survival Data
7 Day Survival Test Used: FISHERS TEST
Control 100% Test Statistic: P= 1.000
Effluent 100% Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Ran Darn
Test for ,4'ormalioy
Mean young/female Std. Dev.
Test Used: Shapiro -Wilke Test:
Control 20.9
2.07
Effluent 20.9
1.93
W:
0.890
Critical Value:
0.884
Analysis for Differences in Reproduction
Test jor Homugeneit}, of Plarionce
Test Used: Equal Variance (Test
Test Used: F Test
Calculated r—
0.00
F=
1.15
Critical Value=
2.51
Critical Value=
5.32
The data are honor eneous in variance
PASS: The effluent is not chronically toxic.
RECEIVED
SEP 0 4 2024
NCDEQ/DWR/NPDES
Page 3 of 6

Control Mortality and Reproduction by Test ..
1189-29
Ise
029-29
37 % Effluent Mortality and Reproduction by Test Day
o
o
1
S Comments 1
NIA -Lost or not used
Page 4 of 6

ETT
:_ F-
Malt
— Box 16414, Greenville, 5C 296n8-7414
[phone], [phone] Faz(864) 877 6938
Shipping Address: 4 Craftsman Ct, Greer, SC 29650
WWW.CrTCNVIR0mmle TAt COM
CHAIN OF CUSTODY RECORD
Client:
C' A .
Program
Containers
Presen•ative
Parameters
Facility:
Whole - muent Toxicity
^
State: C NPDES „: A 297
Acute
Chronic
Test Organisms
a
(Composite only)
(Grab or Composite)
o
=
U
o—
L)
�_
E
n
Z
=
Sign, and Print below
ji
mHCt
`
U
cite dotted line
e
1= =
a
L
U
r
,U
rn
K.
�
=
a-IiNOi
4-N40H
a
2
o
•=
=
o
'
-
'
_�
•v
'-
�
5
�
^'
•=
SAMPLE ID
O
Cempec tr5tart Drle Time
sample Cnllecs on note Tlme
Collected by
U
in rn
Z
x
^
U
i
=
C-ott,
<
<
U
v
j
O
.^
in
U
_
Chemical Analysis B Other
��
w l�-�
zs
- -
x
I
x
1,Jx
q1A
I
( 03�O A
�•__
to
N---------
--- --
0
F-T
---------
-------------
I
III
I
-------------
I
I
III
I
Special Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
I
Date Time RelincLuishedB00ratunization ReceIved,131y / Orzanization
Area
Temp `C
?reserved?
ko
COMPOSfTES,4AIPLING,PROCEDURE'S TE,IIPER:ITURE MONITORING PROCEDURES HOLD TL{IEPROCED[-=
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must first be used mithin 36 hour.
Time Proportional: l sample each hour for 24 hours. Equal volui 0.0 and 6.0'C. Samples must not be frozen. Use water ice in scaled bags. of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 hours. Sample may not be used after'P_ hour-- from sample collection.
Flow Proportional: As per instructions in NPDES permit.

ETT
Pa tax 16AI4. QP[CNVILL[. SC 29606-7414
1a641 a77.694Z, r917131 391-2325 F":18641 877 693e
SNIPPING C--o— CT, alr[[4, 9C 29550
Www.['Tr[N VIPpNM[NT �1-.CON
CHAIN OF CUSTODY RECORD
Page __L_ of
Client:
Program
Containers
Prncrvauve
Parameters
Facility: c
if 5
whale Efnucm T-i6(,
G
❑
State: G NPDES a: Ale i9oky1Tk7
Acute
Chronic
I TnI Or anums
V
F
u
�
uICamposue
Imly)
(Grob or Coctpai;el
<
p
U
p
U
`
❑
C
3.HNO3
O.
L
�'
?
:
D?
E
n
V
Litt
u
U
a E
C
1...,
M
U
s-Na0H
>
o
>
cc
O
E
ra
6
L
a
_
n.^
°
C
J
W
E
V
SAMPLE ID
U
Campni.5—D a Time
S—plrConcatb.nnr Time
Canecledh.
U
n
a
z°
a
7
>
—
6-011.11
<<
U
u
U
C
=
i
n_
U
-
3
i
Chemical Analysis8 Other
w
0Q1e
--ii
lou
x
tj,IA
I
x
Xeo3
0
0,
Special Insrruclions:
Sample Custody Trausfer Record
Secure
Receipt
Sample
Date Time Relinquished By I Organization Received By / Organization b;li gltiln
Area
Temp aC
Preserved"
24
COMPOSITE SAMPLING PROCEDURES TE,4IPERATVRE AfONITORING PROCEDURES HOLD TIbfE PROCEDURES
Composite samples must be collected over a 24 hour period Sample temperature during collection and transpon must be between For toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours. Equal voluf 0.0 and 6.0 PC. Samples must not be frozen. Use water ice in scaled bags. of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in NPDES pennit.
U

tm rwntnonta Inc.
P 0 Box IE414 Greenv lie SC 20606
1,4;:i(nit WAItili 69.311
�rdfl>11!dn Ctx,t, GI_t,. SC.4dti.'
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 12-Jan-22
Laboratory ID #: T60988
Test Reviewed and Approved By:
f/
Ruben W. Kelley, Ph.D.
President
`6
Tw-Certification #E87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NEtAC Certification Number E87819
Florida Dept. of Hwhh. Included results pertam only to provided sam les.
age 1 of 6
Patrick D. Timms
QAIQC Officer
SCDHEC Cerlificatiun ii23194
NCDENR Certification N 022

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 21-Jan-22
Facility: CHARLOTTE WATER Lee S. Dukes NPOES# NCO084387 Pipe # 001 County. Mecklenburg
Laboratory Performing Test. Cam ents
X J `�-
Signature of Operator in Responsible Charge
Xdt
Signature of Laboratory Supervisor
MAIL ORIGINAL TO
CONTROL ORGANISMS
# Young Produced
Adult (Lpve ID)ead
Effluent % 37.0%
TREATMENT 2 ORGANISMS
# Young Pmduced
Adult (LMve (D)ead
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mall Service Center
Raleigh, North Carolina 27699-162
Chronic Test Results
Calculated t=
Critical Value=
3 4 5 6 r 8 9 10 11 12 % Reduction=
-03304
2 508
-1.2%
[
t
23 20 21 22 20 22 17 21 22 24 21 23 is Mortality Avg. Reprod
L L L L L L L L L L L L 0% 21.3
Control Control
� 0% 21 6
eaurienl 2 Treatment 2
Control CV
1 2 3 4 5 6 7 a 9 10 1 t 12 8-6%
% 3rd Brood PASS FAIL
100% X
ro Colets This for Either Tnl Test Start Dale
Cdlecuon iStartt Date 12-Jan-22
It, 1st Bangle 1st sample 2rd sample Sample 1 i 1-Jan-?2 Sample <' 13Jan-22
Control 7.7 81 7.8 8.0
Treatment 2 7.8 7 9 7.8 8.0 7 6 82
21
22
18
23
20
24
21
25
20
22
21
22
L
L
L
L
L
L
L
L
L
L
L
L
start end start end start end
D.O. 1st san a 1st sa a 2nwd sa le
Control
Treatment 2
LC50/Acute Toxicity Test
(Monahty expressed as A. wrnbuvng replicates)
LC50 = %
959� Confidence LinJts
Oraanism Tested
Sample 1 I x
Sample 2 I X
Hardness (nglL)
Spec Cond (pates)
Chlonne (mg/L)
roe Temp. at receipt (*C)
1 sl 2nd
Tax Tox
Dilution Sample Sample
83.3
311 77 81
<.05 <.05
03 0.5
Mortality start/end start end
Method of Determination Control
Averaos Probit I High Conc
an Karter ROther pH D.O.
dubia
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facility: CHARLOTTE WATER NPDY.SO NC0094387
Sample ID: Lee S. Dukes Enw rWea Date: 12-Jan-22
Laborato . Certification #; NCO22 FEp.Date: I I/1J2022
rOval Data
7 Day Survival Test Used: FIS14ERS TEST
Control 100% Test Statistic: P= 1.000
Effluent 100% Critical Value: P= 001
PASS: The effluent does not reduce survival of the test or;anisins.
Reproduction Data
i
—
Raw Data
TesrforNormalio-
it
t
Mean young/female
Sid. Dev.
Test Used: Shaplro-Wills Test:
€
Control 21.3
1.83
Effluent 21.6
1.88
W:
0.966
c
Critical Value:
0.SIs4
inaly:sis for Differences in Reproduction
Tesr for flomogenehgr oft "ariance
Test Used: Equal Variance
t Test.
Test Used: F Test
Calculated t=
-0.33
F=
1.06
Critical Value=
2.51
Critical Value=
5.32
The data are homogeneous to variance
PASS: The effluent is not chronically toxic.
Page 3 of 6

Control Mortality and Reproduction by Test Day
37 % Effluent Mortality and Reproduction by Test Day
0
_�
Comments
Control inl tcm -247
D=Dead N/A -Lost or not used
Page 4 of 6

ETT
PO box 10414, Greenville, SC 29900-7414
[phone], [phone] Fax(864) BT7 6938
Shipping Address: 4 Craftsman CL Greer, SC 29650
W W W.c rrc"V1100"MCNTA-c:0.
CHAIN OF CUSTODY RECORD
Client: I_
Program
Containers
Preservative
Parameters
Facility: (ram
Whole Lmuem Toxicity
State: NC NTDis #: / C t^ o F j
Aeute
khranicl
Test Or onisms
U(Composite
only)
(Grab or Composite).a
`
o
c
E
Z
`~
Sign, and Print below
m
c2.
HCL
[i
N—
•._y
w
_
SAIv1PLE [D
rJ
t3
the doped line
-
C
-
•_
=
�-
U
a_
�?
=
3-HNO3
Zok._
:3
"v:
—5-
`
"
—
Campa:ae Sun Date Time
Sample Callralon Dora Time
Collected by
U
in
in
Z
U
>
G odrr
¢
<
J
v
U
Q
cn
T
U
:-
3
E
Chemical Analysis & Other
C"0
�-l1,Z�-
o
orr.itilcCvzr
X
I
iC
1. rL
X
V
-------------
-------------
-------------
S pcci al Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Relinquished By / Oroanization Received By / Organization
Area
Tem 'C
Presen'ed?
i
v
UUMPUS17E SArtIPLING�ROCEDURES (/ TEWER471IRE A10AUTORING PROCEDURES V 1 HOLD TIME PROCEDURES
Composite samples must collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing ilia sample must first be used %%ithin 36 hour.
Time Proportional: 1 sample each hour for 24 hours. Equal volul 0.0 and 6.0 *C. Samples must not be frozen. Use water ice in sealed baes. of sample collection (completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours. Sample may not be used afters hours from sample collection.
Flow Pronordonol: As Der instructions in NPDES DermiL

ETT
PO Sort 16414, Gretvtvale• SC 298oe-7414
[phone]• [phone] Fax[phone]
Shipping Addrass: a CraRaman G, Greer, SC 29550
.vww, mctir�o.H anrrwt..eo..
CHAIN OF CUSTODY RECORD
n2ge of7771
Client:
)
hl r G 4 lizii
Proenm
Containers
Preser ative
Parameters
` S
Facility:ft
%J
W
<
«Itnm Cmuenc Tosicin
_
-
State: /)/C- M1PDFS ": j (j j �'
.scut,
Chronic I
Tnt O anisms
_
U(Composite
only)
(Grab or Composite)
1
u
Sign, and Print below
>
u
on—
1-1-SO4
y
c
c
❑_
._
._
t
the coned line
e
E
c
U
a.0 o'
u
y
SAMPLEID
U
c.-rw-Sun Det, Time
$ample Canc,tisn Dn, Tim@
Colleetetl by
U
✓
Z
O
ti
�
e-
�
C
,Uj
�.
6. W.,
<
<
U
v
U
L
'=
2
n
-
J
- 3
Chemleal Analysts a Otner
% —/3 -��
'I
0
-------------
-------------
-------------
pedal Instructions:
Sample Custody Transfer Record Secure
Receipt
I Sample
—
Date Time /tiKl iRelin wished By I Organization Received B\ ;Organization IA:ea
Temp `�
?reserved'?
G4y�
_
f . t L, Cv. (./
•� f i
L A,n S r C Itcvc r1 Nj:,�` i�-
�.
I
I
0 S
ga
r
l
COMPOSME SAMPLA'G PROCEDURES
TEhaPE29TURE XfOA7TORIA'G PROCEDURES
HOLD Tl.'•!E PROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For to -icily to=_tine the sample must first be used ts7t)1in 336 hours
Time Proportional: I sample each hour for 24 hours. Equal volts
0.0 and 6.0'C. Samples must not be frozen. Use waier ice in sealed bags.
of sample collection icompleuon of composite sample I.
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used after +2 hours from sarttple collection.
Mow Proportional: As per instructions in NPDES permit.

ET,....nTent.
P 0 Box 1 Ed 14 Greenv llE SC 2 J606
j 4 -4 1 ! , 7 7 i. 7, A '! F-� N , ;;.i t t 1 "? � ;I i "(f
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Fatality: Lee S. Dukes
NPDL-S #: NCO084387
Test Date: 13-Apr-22
Laboratory ID #: T61706
Test Reviewed and Approved By:
4/
Robert W. Kelley, Ph.D.
President
Certification #E87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Cenifieation Number E87919
Florida Dcpt. of Health. Included results pertain only to provided sa Ics.
age 1 of 6
Patrick D. Timms
QA/QC Officer
SCDHEC Certification#13104
NCDENR Certification t, 022
SC :965'

Effluent Toxicity Report Form - Chronic Pass/Fall and Acute LC50
Date 28-Apr-22
Facility: CHARLOTTE WATER Lee S Dukes NPDES# NCO084387 Pipe tr 001 Count Mecklenburg
Laboratory Performing Test: Cdlnnnls
x
c�
Signature of Operator in Responsible Charge
X Alv 4/
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699.1621
North Carolina Ceriodaphnia Chronic Pass/Fall Reproduction Toxicity Test Chronic Test Results
Rank sum= 110.5
Critical Value= 109
CONTROL ORGANISMS
N Ytxtng Produced
Adult (L)rve (D)ead
Effluent %
TREATMENT 2 ORGANISMS
N Young Produced
Adult (Lpve (Diead
1 2 3 4 5 6 7 0 9 10 11 12 % Reduction= 7.4%
22
24
22
22
22
1 24
1 22
22
23
'l4
22
21
EL
L
L
L
IL
IL
L
L
L
L
L
1 7 3 4 S n 7 a o to I 17
23
22
21
19
15
21
22
22
22
20
1 22
1 21
L
L
L
L
L
L
L
L
L
L
L
L
pH 1st sample 131 sample 2nd sample
Control 7.8 8.2 7 7 8 2 7.7 8.1
Treatment 2 7 7 8 1 7.8 7.9 7.8 80
scan end start end start end
D.O. 161 sa a 131 sa a 2nd sa le
Control 7 5 T9 8.0 7 9 T8 8.0
Treatment 2 8 1 8 1 8.3 7 7 81 7 7
LC50/Acute Toxicity Test
(Mortality expressed as %, conlbmung repiicatus)
LC50 = %
954a Con(dancc Lmr1s
Organism Tested
1. This for Elthw Tnl
n tSLanI Dale
12-Apr-22
Sale t
Sample 2 X
Hardness (rtlgil-)
Spec Conti(pmhos)
Chlonno (mgR )
tole Temo at recewt ('CI
0%
Control
22.5
Control
0%
-eatment 2
208
Treatment 2
control CV
4.4%
PASS FAIL
% 3rd Brood
100%
X
Test Start Dale
13-Apr-22
Sample 2 14-Apr-22
151 2nd
T- T—
Dnlutron SamiAe Sample
81 6
302 89 85
<.05 < 05
1.6 0.9
MOrf:Lrl)' swrvend slarlJend
Method of Determination Conovl
A, -crave Probit I High Conc
an Ka ber ROther pH D O
dubia
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Sure ival Data
is
7 Day Survival Test Used: FISHERS TEST
Control 100% Test Statistic: P= 1.000
Effluent 100% Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test jor Nurmalit)•
Mean young1femalc
Sid. Dev.
Test Used: Shapiro-Wilks Test:
Control 22.5
1.00
Effluent 20.8
2.12
W:
0.813
Critical Value:
0.984
analysis for Differences in Reproduction
Test for Ilemogeneity of Variance
Test Used: Wilcoxon Test
Test Uscd: F Test
Rank sum=
110.50
F=
4.51
Critical Values
109.00
Critical Value=
5.32
The data are homogeneous in variance
PASS: The effluent is not chronically toxic.
Page 3 06

.:
----®_�-
r
----®-®-
1124-6
KK3 4-6
.:
abs
Ifenl
T61706
CHARLOTTE WAT
1610
ee 5. Dukes
PDES#
NC0084387
n
ecklen
onth
tart d fed Date
13- r-22
tart 6 fed Time
2.15 PM
tarted d fad By
M
sst ism
eo. born date
erfode ". dubia
12- r-22
to. bom time
eat T
1645.2150
NCCPF
Ilution Water
MHSF
nits for Cona
C
37
3rd BROOD
eH vsssats
set volume
ubator 0
30 ml
15 ml
ht
16tU8dk
Iflaf Tem 'C
24.8
nastrum
0.05 ml
AT
0.05 ml
est method
L. r:, .> a I.
SHARE A CONTROL
Page 4 of 6

ETT _
ru Bax 1ti414, Greenville, SC 29506-7414
C864) 877.6942. [phone]
Shipping Address: 4 Craftsman Cl. Greer. SC 29550
www.atUab.org
CHAIN of CUSTODY RIEcoRD
Pate J_ 0f___L
Client: [rka. lD 4-yc-
W q r
Eaellity:
Program
Coraaincrs
Prescn-atit
c
Parameters
L-u- 5. icw, c s v], •TV
Whole Lm���t Toxicity
—
n
State: NC— NPDCSR: IJG O C7 � `t 3$'j
Acute
Chronic
Test Or onismc
-
(J
(Composit_only)
(Grob or Composite
-
O
J
F
Si;n, and Print below
u
c
2-11Ct
—
_
c
_
SAMPLE ID
E
Ole dotted line
ZJ
,g
°
3-tn,+os
=
=
-
—
o
camp•sntsmn ua,c Time
sampicCutlmion Date Time
Caileded by
Ci
n
z
i=
O
y
s-a,At c- od„T
<
<
U
U
U
Q
•_
2
in
._
v
-
Chemical Analysis s Other
0 (0 {
4113 1'�L
075/
—
)(I
I
i
k
NSA
I
X
i(I
AIerc,JwaP
_
(p(7�r�
to-------------
-
-
-
-------------
THT
ITI
M
Ll
I I
Special Instructions. _
Sample Custody Transfer Record
Secure
( Receipt
I Sampic
Date Time Relin uished El / Organization
f�efceivedy reanizalion
z-t—,
Tem❑ `C
Presen•cd7
•-1 i11da
CSa`}
p
Gh C r,lr A•i,` l J c, it r [ ec 17 :1cts
/
/ L>'l r �a i✓�L,r
I
J
1
F-[j'
12Y
�+ LO
COAIPOSITE SW IPLIA'G PROCEDURES
TEAIPER�iTURE ,b10A11TORING PROCEDURES
HOLD T1AIE PROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For Toxicity testing the sample must first be used mithin =6 hours
Time Proportional: I sample each hour for 24 hours. Equal voles
0.0 and 6.0 °C. Samples Must not be frozen. Use water ice in scaled bags.
of sample collection (completion of composite sample I.
or it minimum i sample every 4 hours over 24 hours.
Sample may not be used after T hours. from sample collection.
Plow Proportional: As per instmclions in NPDES permit.

ETT
4_.. �en�v�cnm�nta
PO 80X 16414, Greenville. SC 29506-7s1A
1884) 877-5941. [phone]
Shipping Address: 4 Cralnsman Ct. Greer, SC 2960
lrmr-enlab.uril
CHAIN OF CUSTODY RECORD
Page i ofJ_
Client: L h ct.r 10 +4-(— W ' C' +Cr'
"ram
Containers
Presen•aclve
Parameters
Facility: LG� S PIt KC S
Wbola Emueat Toiidry
=-
—
State: Nr Ci NPDL• S N: N ' L '.;' < '�''? �. !
Acute
Chnale
Test Organisms
U(Composi
te oniv 1
(Grib or Composim)
a
L
�
`
CJ
ii
,.
yF
Z
C
Sign, and Print below
e
o
c
a
I-FCS04
?•MCL
_
L
.Y
e
__
_
=
=
'
c
a
f
die donedline
=
5
?
^e
u
.�
J
oNr
u
-Ee�__
e
=
r?
Y
e
-=�-_�
—
SAMPLE ID
t3
Ceryas Yssun Dar Time
5 mvk Collwwn Dam Time
Colleacd by
iJ
h
y
Z
na:
Q.
t7
>
—
l•znAc
H
u
<
<
U
8
C.i
C`
c
L
1 :2
—
f
-
U
=
+
3
Chemical Analysis & C or
_
0 0 l
1+11111` 11 �L
-
1�
i �`�
�`
N 1 A
X
rl-- -�„--Tins
a
10
--- ---
- -
__IT
-------------
-------------
!
i
Special Instructions:
Sample Custody Transfer Record
Secure
Receipi
Sample
Date Time Relinquished Byk Organization ivied By I Orunization
Area
Temp °C
Preserved°
47-
COMPOSITE SAMPLING PROCEDURES rEA.L-ER.ITURE ,MONITORING PROCEDURES .HOLD TV fE PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing die sample must first be used icidiin 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volui 0.0 and 6.0'C. Samples must not be frozen. Use water ice in sealed bags. of sample collection (completion ofcomposite samplei-
or at minimum I sample even• 4 hours over 24 hours. Sample may not be used afier 72 hour, fram sample collection.
Flow proportional: As per instructions in NPDES penmil.

ETr.,pita inc. (L(:41 O,7fny iI1(4107� lia.t{
PO Box IE414 Gteenv lle SC 20606 G dfi,rndn Ct.LI. Gt :f t 5C 1:tdit
Ceriodaphnia dubia Survival and Reproduction "Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 1.1-Jul-22
Laboratory ID #: T62418
Tat Reviewed and Approved By:
Robert W. Kelley, Ph.D.
President
Certification #E87819
Test results presented in this mTon conform to all rctlaire ents of
NELAC. conducted under NELAC Certification Number E87819
Florida Mpt. of Hcalth. Includwt results pertain only to prosided samplage 1 of 6
Patrick D. Timms
QAIQC Officer
SCDHEC Certification#23104
NCDEN R Certification # 022

Effluent Toxicity Report Form - Chronic Pass/Fall and Acute LC50
Date 25-Jul-22
Facility: CHARLOTTE WATER Lee S Dukes NPDES# NCO084387 Pipe a 001 County Mecklenburg
Laboratory Performing Test
Signature of Operator in Responsible Charge
X
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch
Div. o1 Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
CONTROL ORGANISMS
a Young Produced
Adult (LNve ID)ead
Effluent % 37.0°k
TREATMENT 2 ORGANISMS
a You Produced
Adult (LA a (D)ead
pH
Control
Treatment 2
nk sum= 130.5
tical Value= 109
°
1 2
3
4
5
6
7
8 9 10 11
12
% Reduction= 0.8 /e
21
20
19
23
23
22
23
23
21
21
22
22
90 Malallty
Avg. Reprod
L
L
L
L
L
L
L
L
L
L
L
L
0%
217
Control
Control
0%
21 5
Treatment 2
Treatment 2
Control CV
t 2
3
4
5
6
7
8 9 10 11
12
6.0%
21
21
23
21
20
30
22
1 18
1 21
1 22
20
19
%3rd Brood
PASS FAIL
L
L
L
L
L
L
L
I L
I L
L
L
L
100%
X
plate TN* for Either Teat
Test Start Dale
t3-JLA-22
121u1 22
14-J A-22
1st sample till sample 2nd sample Sande 1 Sample 2
7.8 8.2 7.7 80 7.6 8.1
8.0 8.2 77 80 7.7 80
slam end start and Stan end
D.O. 161 sa a 151samoe 2nd a
Control 9.0 8 3 8.2 8 6 7.6 8 7
Treatment 2 9.4 8 4 8.4 8 4 7 8 8 9
LC50/Acute Toxicity Test
IhiorlahtY expressed as % mmtxnjN repluratm
Sarrlple t
Sarnpo 2 7i
Hardmess (mg2)
Spec Coed (pmMs)
CNonne(mg0.)
XAe Temp at receipt I'C)
154 2nd
Ton To.
D h'Wn Sample Sample
81.6
303 95 83
<05 <05
1.5 15
Mortality 5W NCnd SlaNand
LC50 = % Method of Determination c« t a
IS°e Confudanca Limits Larman
mp Average rObll H-j FOh C°nc
Itareer Fqther
pH D O
Organism Tested Ceriodaphnia dubia
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facilit : CHARLOTTE WATER
NPDUA NCOO94387
Sample ID: Lee S. Dukes
errr relate Daic: 13-Jul-22
Laborato .
Certification tf: NCO22
Exp. Date: 11/1/2022
Sur%ival Data
7 Day Survival
Test Used: FIStIERS TEST
Control 100%
Test Statistic: P= I.000
Effluent 100%
Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Ran Data
TestfarNormaliq
Mean young4emale
Sid. Dev.
Test Used: Shapiro-Wilks Test:
Control 21.7
1.30
Effluent 21.5
I
3.00
W:
0.793
Critical Value:
0.884
-t nalpsis for Differences in Reproduction
Tat for Homogeneity of variance
Test Used: Wilcoxon Test
Test Used: E Test
Rank sum=
130.50
F=
5.30
Critical Value--
109.00
Critical Value=
5.32
I he data are honiatzeneous In variance
PASS: The effluent is not chronically toxic.
Page 3 of 6

Control Monalky and RwoducuDn try Test ..
6-30
27.1
16-30
1
�
®-__----
�
fil►iiY
r1a':S"�
ments
18 d 62419 SHARE A CONTROL
=Dead WA -Lost or rot
Page 4 of 6

ETT
PO Bm[ 16414, WeermYle, SC 29506--7414
(864) BT7-6942, [phone]
SNpping Address: 4 C-MItsman Ct, Greer, SC 29650
www etllab.arg
CHAIN OF CUSTODY RECORD
2ZC I 0' I
Client: f
C Hart V vl'� r
Program
Containers
Preservative
Parameters
Facility: �tnICQ.1�
4'holc E01ucn Tasicin•
—
State: e- NPDES R: fV C 0 0
Acute
Chronic
Test Ort.,anisms
—
L
d
:/
—
—
U
(Composite only)
(Grob or Compo:itel
<
D
o
=
`
_
-�
,
v
?
-,
Xt
L
Sign, and Print below
3
u
m
NCL
—
'
—
r'
zr
die dotted line
=
r
u
'�
;•tuao;
=
—
,�
_
SAMPLE ID
comp.sire Sun Dare Time
Semple Col4ttian arre Ttme
Colleted b1•
U
v
'n
Z
o
c
U
>
�, 0e�
<
<
J
v
^
=
2
-
J
.=
i
2
Chemical Analysis 3 Other
G-12-i"
o
lvc-n h1cEver
u
1
k
1.9L
x
/q
k
�l1`�
ro
Ln
rn
I
--------------
Speci ztI Instruction s:
Sample Custody Transfer Record
Secure
Rcccipi
Sample
Date Time Relin wished B /Or,anization A Recciv4d BIN,/ manization
Area
Tem `C
Presen•c,i''
i-1'Z�12
092-0—
l� nilia"
77
COA,(POSIT-E SAMPLING PROCEDURES TEAIPER4TURE ,t /OAr1TOR1AIG PROCEDURES HOLD TIME PROCEDU-ES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must first be used within 36 hour.
Time Proportional: I sample each (tour for 24 hours. Equal volui 0.0 and 6.0'C. Samples must not be frozen. Use water ice in scaled bags. of sample collection (completion of composite sample).
or at minimum I sample every4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in NPDES permii.

ETT
PO Bmc tB4t�, Grearwitla, SC 29eo8.741a
(BM) 577-8942. (5W) 891-2325
Shipping Morns: 4 Craftman Q. Greer. SC 29650
www.eltlab.ar g
CHAIN OF CUSTODY RECORD
Client: I_ f�
�'�1l�-zr
Program
Containers
Prescnati,c
Paramerers
Facility: Lam- S D�k�s wTh
�'
C
Whole Unucm Tavc,n•
_
_
—
_
State: N NPDES 9: 0
+cute
Chronic
Tnr O ao sns
c
(Compmneonly)
IGraborCompoeitci
-
3
•-
_
Sign, and Printbclo+r
3
=
=
n
o
:-u[[
_
_
—Z
u
the dotted Tine
=
^_
c
E
O
?=
O
j
S- Znac
J
J
-
-
_
-
-
•-
I }•
_
-
=ZZz
_
SAMPLE !D
�?
Camp•na sun 0a a Trms
Sample CNteaioe o.m Timo
Collected by
U
n
Vol
Z
a
_
J
—
�. pp-
<
<
—
v
❑
_ 2
J
=
—
_
L
Chem'cal Analysis 8 Caner
CT
- _ _
X
%
?C
0LX�1
cot
-I -�Z
O
av 1 h1cEvCr
to-------------
0
-------------
-------------
Special Instructions.
Sample Custody Transfer Record
Secure
I.Alea
Semple
Receipt Semple
Date Time Relinquished B1 I Organization Received Br 1 Oreanizatior,
Date
Temo :C
1 2�
gSj
/1 I t -rn acA -1 I F
I
COMPOSITES,4A1PL1YG PROCEDURES TELIPEILtTURE ,1/ONITORI,\,G PROCEDURES VOLD 71.1lE PROCEDLRZ-7
Composite samples must be collected over a 24 hour period. Sample temperature during collection and tronspon must be bemeea For toxiciq testing dre sample must first be used ++stain 56 hours
Time Proportional: I sample each hour for 24 hours. Equal volui 0.0 and 6.0 *C. Samples must not be frozen. Use ++ater ice in sealed bads of sample collecrion 1 compl_-lion of composrre sampler
or at minimum I sample every 4 hours over 24 hours. Sample mad not be used after 72 hour from sample collection.
Flow Proportional: As per instructions in WDES permit

Ecn,,.nenwiu Inc. _ I
rO Box 1EA14 Oteenvtie SC 29606 :ita -..t'.'t 'Sc 'm
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 12-Oct-22
Laboratory ID #: T63178
Test Reviewed and Approved By:
Robert W. Kelley, Ph.D.
President
Certification NES7819
Test results presented in this report conform to all requirements of
NELAC, conducted under NElAC Certification Number E8781 v
Florida Dept. of Health. Included results pertain only to provided mrn Ics.
age 1 of 6
Patrick D. Timms
QA/QC Officer
SCDHEC Certification #23104
NCDENR Certification# 022

Effluent Toxicity Report Form - Chronic PasWFall and Acute LC50
Date 24-Oct-22
Facility: CHARLOTTE WATER Lee S. Dukes NPDES# NCO084387 Pipe 0 001 County Mecklenburg
Laboratory Performing Test: comments
X
Signature of Operator in Responsible Charge
X
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodaphnia Chronic Pass/Fall Reproduction Toxicity Test Chronic Test Resufts
Calculated t= 04838
Critical Value= 2 508
CONTROL ORGANISh15 1 2 3 4 5 6 7 6 9 10 11 12 % Reduction= 1 2%
p Young Produced
Adult (LNre (plead
Effluent %
TREATMENT 2 ORGANISMS
M Young Produced
Adult ILlrve (plead
20
22
22
1 20
21
22
22
20
17
21
20
22
L
L
L
L
L
L
L
L
L
L
L
L
1 P a to 11 12
i9
21
20
20
21
20
19
21
22
21
20
22
L
L
L
L
L
I L
L
L
L
L
L
L
pH 131 Sample 151 Sample 2nd sample
Control 7 7 8.1 7.8 8.3
Treatment 2 7.6 8.0 7.8 8.4
slant and start end Stan end
D.O. tat a 1st sa le end sa a
Control 8.2 8.2 8.0 7.8
Treatment 2 8.3 8.0 8.6 8.0
LC50/Acute Toxicity Test_
(Monalmy expressed as a, combining replicates)
LC50 = %
95% Confidence LIMIS
Draanism Tested
to This for Either Test
In iSLaril Dale
1 11-0c1-22
sample t x
Sample 2 ),
Hardness (mglL)
Spec Cond (vmhos)
Chicinne (mgiL)
role Terre. at recela VC)
0%
Control
20.8
Coned
0%
"ealment 2
205
Treatment 2
on" CV
7,2%
PASS FAIL
L16f-000
X
Toot Start Date
12-ocl 22
Sample 2 13-Oct-22
lit 2nd
Tax Tax
Dfution Sample Sample
85.7
322 87 89
<0.05 1 0.08
11 1 1.8
Mpr coy slamend starvend
Method of Determi
nation contra
A.erat7e robit Hgh Cone11
an Kerber pH D O
dubia
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facility: CHARLOTTE WATER
a°ui -•.- NCO084397
Sample ID: Lce S. Dukes
LTTa T63173
Date: 12-Oct-22
Laboratorv.
Certification N: NCO22
I Exp. Date: 11/112022
Survival Data
7 Day Survival
Test Used: FISHERS TEST
Control 100%
Test Statistic: P= 1.000
Effluent 100%
Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test jor Normality•
Mean young/female
Sid. Dev.
Test Used: Shapiro -Walks Test:
1
-
,f
Control 20.8
1.48
' Effluent 20.5
i
1.00
W:
0.884
a
t
Critical Value:
0.884
9naij,sls fur Differences in Reproduciian
Tart jor Romogenehy of Variance
Test Used: Equal Variance t TesL
Test Used: F Test
Calculated t=
0.48
F=
2.20
Critical Value=
2.51
Critical Value=
5.32
The data are homo eneous in rariance
PASS_ The effluent is not chronically toxic.
Page 3 of 6

..
�!
9-30
1-2-19
37 % Effluent Mortaty, and Reproduction by Test .:
bi
lient
T63178
CHARLOTTE WAT
N ID
Lee S. Dukes
PDES#
NC0084387
oun
Mecklenburg
onth
10
tart A fed Date
12-Oct-22
tart d fad Tfma
3 20 PM
tarted & fad 8Y
AM
eat anlom
Ceriodaphnki dubia
so. bom data
11-0ct-22
eo. bom Uma
1700-2200
sot Type
NCCPF
lutfon Water
MHSF
nits for Conc.
C
37
BROOD
sat vessels
30 ml
psi volume
15 ml
ubalor S
1
ht
161U8dk
Mal Tom 'C
24.8
danastnrn
0.05 ml
AT
005ml
eat madvod
(P. .24d]a�]ioul
Dead NIA -Lost or not used
Page 4 of 6

ETT
&MIR-20101
===== �-=7-
PO &1x 16414. Greenvile, SC 29606-741A
[phone], (ODD) 891 -2325
Shipping Address[ 4 Cransman Cl, Greer, SC 29650
www.ahlab.org
CHAIN OF CUSTODY RECORD
Page I of i— I
Client: I
1'V
Program
Facility.
Containers
Preservative
P.1 ra mCI
C ri
1Yhvhe L• mucnt Tosidtt•
'�
c
State: tiPDLS �:
.Acute
Chronic
Test Organisms
-
4
U(Composite
only)
(Grab nr Composite)
<
o
c
_
U
t
Sign, and Print below
3
}
.
o
2-14CL
_^
-
=
e
SAMPLE ID
U
file dotted line
-
E
r
v
.�
=
s•H�os
>,
=
=
'_
__
n
=
_
z
iz
.�
_
Campsslestm Datc Time
Sample Collerinn Dn a Time
Collected br
U
s
C4
Z
C
(]
?
=s
i=2't'e
(r OJss
<
<
U
U
U
O
=
2
�'
Chemical Analysts &Other
QO
�3�t,�z��a�----
---- ---
a
X
NIA
X
x
U3
U
M
-------------
MT
IM
-------------
I
I_-- ----------
Special Instructions: —
Sample Custody Transfer Record
Secure
(Area
Receipt
I
Sample
Date Time wished yl ece
anization Rived i v Or�tnization
r
Temp'C
I Presen'ed7
I01111aa VOL, G�fi Wrtu — Ltz S. Dj1<-cS W t?
x��
1 D-. P
(4
COMPOSM S.4HPLING PROCEDURES
TEA fP£RATURE MONITORING PROCEDURES
HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity' testing the sample must first be used %%thin 36 hours
Time Proportional: I sample each hour for 24 hours. Equal volut
0.0 and 6.0 *C. Samples must not be frozen. Use water ice in sealed bars_
of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Plow Proportional: As per instructions in NPDES permit,

ETT
P Box 111, Lin"WIas. SC 2960&7414
(864) 8T7-6942, [phone]
ShIppin9 MOMS: 4 Craftsman CL Grw. SC 29850
www.eltlabar•p
CHAIN OF CUSTODY RECORD
Pale — of �
Client: n!
Program
Containers
Presenativc
Parameters
Facility: eG �rS
1Phole Cmucm Tasicin-
fate: Ak NPDES',: A16 ookY JL7
Acucc
Chronic
TMI 01_1enismt
(Compndle only)
(Grob or Componce)
<
Z'
'�-
V
_
—
rJ
Z
U
°
_
—_
—
=
Sign, and Plint below
3
�
v
m
c
�
1-H2SO4
='Hcu
u
Z/1
t11e doped line
d.LbOM
SAN�LE ID
G
Conmmiu Sbn Dus Tlme
S.mpk Cotlema. Dew Time
Ca c ln•
U
h
cn
Z
x
a
_
7
>
—
n. oth ,
4
S
Cttemlcal Analysis L Other
d
��
z
075o
A
d
m
-------------
C,
-------------
-------------
Special Instructions:
Sample Custody Transfer Record
Secure
Receipi
Sample
Date Time RclinclWahrd By / Organization Rccci cd By t Oreanization
,tea
Temp `C
Presened'i
COMPOSIT£3.4MPLING PROCEDURES TEA1PER.4TURE A10-MITORING PROCEDURES HOLD 71:19E PROCEDURES
Composite samples must be collected over a 24 hour period. Sample temperature during collection and transport must be between For tocicit. testing the sample must first be used N%iotin :! hours
Time Proportional: 1 sample each hour for 24 hours. Equal volui 0.0 and 6.0 *C. Samples must not be frozen. Use water ice in sealed bags. of sample collection (completion of compostle sample).
or at minimum I sample every 4 hours ovrr 24 hours. Sample map not be used after 72 hours ftom sample collection.
Flmv Proportional: As per instructions in NPDES permit.

cnv ronmCnt� ,Ina
RO. Box 9 E,t 14, Greenvlle, SC 29606
(E64) 377-6942 . FAX [phone]B
4 Ciatlsrndn Cuuri, Greer, SC 29650
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Client: Lee S. Dukes
NPDES #: NCO084387
Test Date: 04-Jan-23
Laboratory ID #: T63805
Test Reviewed and Approved By:
41
Robert W. Kelley, Ph.D.
President
Certification #E87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Certification Number E87819
Florida Dept. of Health. Included results pertain only to provided samples.
Page 1 of 6
re;'�-4 -�>. d",
Patrick D. Timms
QA/QC Officer
SCDHEC Certification #23104
NCDENR Certification # 022

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 20-Jan-23
Facility: CHARLOTTE WATER Lee S. Dukes NPDES# NCO084387 Pipe # 001 County. Mecklenburg
Laboratory Performing Test: Comments
X i"�"!� �GL�
Signature of Operator in Responsible Charge
X�
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodaohnia Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t= 1.1639
CONTROL ORGANISMS
# Young Produced
Adult (L)ive (D)ead
Effluent %
TREATMENT 2 ORGANISMS
# Young Produced
Adult (L)ive (D)ead
Critical Value= 2.508
1 2 3 4 5 6 7 8 9 10 11 12 % Reduction= 1.9%
21 22 22 21 22 20 22 22 20 22 21 22 % Mortality Avg. Reprod.
L L L L L L L L L L L L 0% 21.4
Control
Control
0%
21.0
Treatment 2
Treatment 2
Control CV
0
1 2 3 4 5 6 7 8 9 10 11 12 3.7/0
20
21
22
22
20
21
22
21
21
19
22
21
L
L
L
L
L
L
L
L
L
L
L
L
pH 1st sample 1sl sample 2nd sample
Control 7.7 7.9 7.8 8.3 7.7 8.1
Treatment 2 7.9 8.1 7.9 8.4 7.7 8.3
start end start end start end
D.O. 1stsam le 1slsam le 2nd sam le
Control
Treatment
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
to This for Either Test
,n (Start) Date
1 03-Jan-23
Sample 1 X
Sample 2 X
Hardness (mg/L)
Spec. Cond.(pmhos)
Chlorine (mg/L)
iDle Temp. at receipt (`C)
% 3rd Brood PASS FAIL
100% FX
Test Start Date
04-Jan-23
Samole 2 05-Jan-23
list 2nd
Tox Tox
Dilution Sample Sample
82.0
304 79 83
<0.05 <0.05
0.6 0.9
Mortality slam/end start/end
LC50 = % Method of Determination Control
5% Confidence Limits Moving Average Probit High Conc.
Spearman Karber ROther I pH D.O.
Organism Tested Ceriodaphnia dubia
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facility: CHARLOTTE WATER NPDESS NC0084387
Sample ID: Lee S. Dukes ETT# T6380S Date: 04-7an-23
Laboratory Certification #: NCO22 Exp. Date: 11/1/2023
Survival Data
7 Day Survival
Test Used: FISHERS TEST
Control 100%
Test Statistic: P= 1.000
Effluent 100%
Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test for Normality
Mean young/female
Std. Dev.
Test Used: Shapiro-Wilks Test:
Control 21.4
0.79
Effluent 21.0
0.95
W:
0.899
Critical Value:
0.884
Analysisfor Differences in Reproduction
Test jor Homogeneity of Variance
Test Used: Equal Variance t Test.
Test Used: F Test
Calculated t=
1.16
F=
1.45
Critical Value=
2.51
Critical Value=
5.32
The data are homogeneous in variance
PASS: The effluent is not chronically toxic.
Page 3 of 6

Control Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
source
rep
G212-22
1
4
+8+9
21
A312-23
2
5
+7+10
22
0912-22
3
4
+7+11
22
G112-22
4
+3+8
10
21
D5 12-23
5
+4+7
11
1
22
N8 12-22
6
+3+7
10
20
K712-22
7
4
+7+11
22
E212-23
8
4
+7+11
22
M612-22
9
+3+8
9
20
K9 12-22
10
+3+9
10
22
F4 12-23
11
+3+7
11
21
Q3 12-22
12
+4+7
11
22
13
N/A
0
14
N/A
0
15
N/A
0
16
N/A
0
17
N/A
0
18
N/A
0
19
N/A
o
Mean
20
N/A
0
21.4
37 % Effluent Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
G2 12-
1
+4+6
10
20
A3 12-2
2
+5+6
10
21
09 12-2
3
5
+8+9
22
G1 12-
4
+4+7
11
22
D5 12-2
5
+3+7
10
20
N8 12-2
6
+3+8
10
21
K7 12-2
7
4
+7+11
1
22
E2 12-2
8
+3+8
10
21
M6 12-
9
+4+6
11
21
K9 12-2
10
4
+6+9
19
F4 12-2
11
5
+7+10
22
Q3 12-2
12
+4+7
10
21
0
13
N/A
0
0
14
N/A
0
0
15
N/A
0
0
16
N/A
0
0
N/A
0
N/A
0
0N/A
Ll
o
Mean
0
N/A
0
21.0
renew-
JG
JC
fed
Jc
JG
JC
AM
Jc
JC
11-Jan-23
time fed & renew --
11:45 AM
01.48 PM
06,15 AM
011:00 AM
11:07 AM
07:24 AM
10.49 AM JC
New temp. °C
24.8
24.8
Old temp.'C
25.21
1
25.2
24.8
Control New temp.'C
.24.51
1
24.7
Lab#
Client
T63805
CHARLOTTE WATE
Sample ID
Lee S. Dukes
NPDES#
NCO084387
County
Mecklenburg
Month
1
Start & fed Date
04-Jan-23
Start & fed Time
02:10 PM
Started & fed By
JC
Test Organism
Ceriodaphnia dubia
Neo. born date
03-Jan-23
Neo. born time
Test T pe
1700-2200
NCCPF
Dilution Water
MHSF 12/29/22A
Units for Conc.
%
IWC
37
%3rd BROOD
Test vessels
30 ml
Test volume
Incubator#
15 ml
1
Light
Initial Temp °C
161t/Bdk
24.8
Selenastrum
0.05 ml
YAT
Test method
0.05 ml
JEPA 821-R-02-013:1002
ini tem
Comments
or not used
Page 4 of 6

ETT
PO Box 16414, Greenville, SC 296DB-7414
[phone], (OW) 891-2325
Shipping Address, 4 Craftsman Cl, Greer, SC 29650
WWW.Eitiob.org
CHAIN OF CUSTODY RECORD
Client:
W(,tl—IT� W k1r4,R
Program
Containers
Preservative
Parameter.
Facility: S 1) U K i5 W f T
� 1
<
J.
U
E�
O
rn
c
rn
C`
o
v
C
O
Z
12
�_
o
u
4
O
�
•—'
N
C
r-
c
U
O
>
0
3=HCL
a�nooH
6?0 -
Whole GmaontToxicity
u
—
?
7
State: NPDES 4: (`� pj�
Acute
Chronic
I Test Organisms
SAMPLE ID
E
U
o
�
(Composite only)
Composite Stan Dote Time
(Grab or Composite)
Sample Collcctian Dme Time
Sign, and Print WOW
the dotted lisle
Collected by
=
L
—
�,
d
o
U
�
4
y
2
CJ
6
U
o
CJ
=
U
,m
E
or
Z
b
_
.0
2
'R=
_t'
rn
c
T
o
AJ
b 1
G
- -- ----
X
1"q
X
dA
-------------
LL-
Special Instructions:
Sample Custody Transfer Record wv/e. reLt3*icv1 4,} Cl,� rlc,4?- V-+rJ f.SF
Date Time RQIiiiquislied B / Organization Received By / Orpanization
l/3 /z
091q
3
A.a
COMPOSITE SAMPLING OCEDURES
Composite samples must lVeollected over a 24 hour period.
Time Proportional: 1 sample each (tour for 24 hours. Equal volui
or at minimum 1 sample every 4 hours over 24 hours.
Flow Proportional: As per instructions in NPDES permit.
TEMPERATURE MONfTORING PROCEDURES
Sample temperature during collection and transport must be between
0.0 and 6.0'C. Samples must not be frozen. Use water ice in sealed bags.
010LD TIME PROCEDUI
For toxicity testing the san
of sample collection (coml
Sample may not be used a
Page 5 of 6

ETT
PO Box 15414, Greenville, SC 29606-7414
[phone], [phone]
Shipping Address: 4 Craftsman Ct, Greer, SC 29650
www.ohlob.org
CHAIN OF CUSTODY RECORD
Client: Q
"titer
't t
Program
Containers
Preservative
Parameter.
Facility: L�� S _ U1CLS W-if
Whole Emucot Toxicity
State: NPDCS #: N C,g
Aeatc
lChronic
I Tat Organisms
=
cL
c
U
(Composite only)
(Grab or Composite)
u
<
O
k
si
_
G
U
U
_
t3
O
U
•...
i
O
o
Sign, and Print below
die dotted line
3
=
3
^e
m
o`
U
o
c
1-ri2504
i-liCt
3-I4No;
=
'—
'
•c
_
a=
•=
u
'—
SAMPLE ID
v
�?
Composite stnn Da,e nma
Smaple Coaenlon Date rime
ColledeJ by
a
o
U
o_
h
rn
.-
z
.-
a
H
J
>
J=NaOH
e �
<
Q
o
(�
o
U
U
E
C
r=
N
o—
in
T
o
U
F
3
001
D I 05 23
lS
'�l J C waI1
Special Instructions:
Sample Custody Transfer Record
Date Time Relinquish B / Organization Received B / Organ" • n
s 3'�
Loin/
�t iL � . � C.0 i ✓L [C h I GtfGY r
s
!0oq
Crhai 16 He
�s
IIS*lj 3
JS
w
OMPOSM SAMPLlN ROCEDURES
TEMPERATURE MONITORING PROCEDURES
HOLD TIME PROCEDUI
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the san
Time Proportional: 1 sample each hour for 24 hours. Equal volui
0.0 and 6.0'C. Samples must not be frozen. Use eater ice in sealed bags.
of sample collection (coml
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used ai
Flow Proportional: As per instructions in NPDES permit.
Page 6 of 6

TC".,,T0n-,n-. t5G4 j 877 6S i2 . FAX(b64) Ei'7 G93E3
R0. Dox 1 E.414, Greenvlle, = 29606 4 Ciaflsrrlan Court, Greer, SC 29G50
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 05-Apr-23
Laboratory ID #: T64525
Test Reviewed and Approved By:
44.71 414.
Robert W. Kelley, Ph.D.
President
Certification #E87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NEI.AC Certification Number E87819
Florida Dept. of Health. Included results pertain only to provided samples.
Page 1 of 6
Patrick D. Timms
QA/QC Officer
SCDHEC Certification #23104
NCDENR Certification # 022

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 14-Apr-23
Facility: CHARLOTTE WATER Lee S. Dukes NPDES# NCO084387 Pipe # 001 County. Mecklenburg
Laboratory Performing Test:
Comments
X Da� { ljah�
Signature of Operator in Responsible Charge
X
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodanhnia Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t= 0.4208
CONTROL ORGANISMS
# Young Produced
Adult (L)ive (D)ead
Effluent %
TREATMENT 2 ORGANISMS
# Young Produced
Adult (L)ive (D)ead
Critical Value= 2.508
1 2 3 4 5 6 7 8 9 10 11 12 % Reduction= 1.2 %
20
23
21
22
20
21
22
1 20
21
19
23
21
L
L
L
L
L
L
L
L
L
L
L
L
1 2 3 4 5 6 7 R 9 1n 11 19
20
23
20
20
20
21
22
20
24
18
20
22
L
L
L
L
L
L
L
L
L
L
L
L
pH 1st sample 1st sample 2nd sample
Control 7.8 S.0 7.8 8.3 7.7 8.2
Treatment 2 7.6 8.0 7.9 8.3 7.8 8.1
start end start end start end
D.O. 1sl sam le 1st sam le 2nd sam le
Control
Treatment 2
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
LC50 = % Method of Determination
95% Confidence Limits Moving Average Probit
Spearman Karber ROther
Orqanism Tested Ceriodaohnia dubia
to This for Either Test
,n (Start) Date
1 04-Apr-23
Sample 1 X
Sample 2 X
Hardness (mg/L)
Spec. Cord. (pmhos)
Chlorine (mg/L)
iple Temp. at receipt (°C)
0%
Control
21.1
Control
0%
reatment2
20.8
Treatment
ontrol CV
5.9%
PASS FAIL%
L3rdroad
X
Test Start Date
05-Apr-23
Sample 2 06-Apr-23
1st 2nd
Tox Tox
Dilution Sample Sample
84.0
317 82 0
<0.05 <0.05
1.9 1.2
Mortality start/end starUend
Control
QHigh Conc.
pH D.O.
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Survival Data
7 Day Survival Test Used: FISHERS TEST
Control 100% Test Statistic: P= 1.000
Effluent 100% Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test far Normality
Mean young/female
Std. Dev.
Test Used: Shapiro-Wilks Test:
Control 21.1
1.24
Effluent 20.8
1.64
W:
0.956
Critical Value:
0.884
Analysis for Differences erences in Reproduction
Test for Homogeneity of Variance
Test Used: Equal Variance t Test
Test Used: F Test
Calculated t=
0.42
F=
1.75
Critical Value=
2.51
Critical Value=
5.32
The data are homogeneous in variance
PASS: The effluent is not chronically toxic.
Page 3 of 6

3urce
rep
Control Mortality and Reproduction by Test Day
1 2 3 4 5 6 7
H7 3-22
1
+3+7
10
G3 3-22
.7 3-23
13 3-23
2
+3+9
11
3
+3+8
10
4
+4+7
11
1 3-24
4 3-24
1 3-24
5
+3+7
10
6
+4+7
10
7
4
+6+12
7 3-24
8 3-24
10 3-24
8
3
+6+11
9
4
+7+10
10
+4+6
9
5 3-23
2 3-23
11
+4+8
11
12
+4+6
11
16
17
18
8
220
23
21
22
20
21
22
20
21
19
23
21
0
0
0
0
0
0
HH7 3-22
GG3 3-22
37 % Effluent Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
1
+4+6
10
20
2
+5+7
11
23
Q7 3-23
3
+4+7
9
20
M3 3-23
S1 3-24
4
+5+6
9
20
5
+4+6
10
20
S4 3-24
6
+3+7
11
21
U1 3-24
A7 3-24
A8 3-24
7
4
+7+11
1
22
8
+3+7
10
20
9
4
+8+12
24
F10 3-24
G5 3-23
G2 3-23
0
0
10
+3+6
9
18
11
+4+7
9
20
12
5
+7+10
22
13
N/A
0
14
N/A
0
O
15
N/A
0
0
0
16
N/A
0
17
N/A
0
0
18
N/A
0
0
19
N/A
0
0
20
N/A
0
renew Initial
JG
JC
End Date
fed by
JC
JG
JC
AM
JC
JC
12-A r-23
time fed & renew
09:12 AM
01:07 PM
06:00 AM
07:00 AM
09.49 AM
07:29 AM
09:56 AM JC
24.8
P24.6
. °C
25.1
249
25
t •c 1 24 66
24.4
24.4
Lab#
T64525
Client
CHARLOTTE WATE
Sample ID
Lee S. Dukes
NPDES#
NCO084387
County
Mecklenburg
Month
4
Start & fed Date
05-Apr-23
Start & fed Time
Started & fed By
12:30 PM
AM
Test Organism
Ceriodaphnia dubia
Neo. born dale
04-Apr-23
Neo. born time
1645-2150
Test Type
NCCPF
Dilution Water
Units for Conc.
MHSF
%
IWC
37
%3rd BROOD
Test vessels
30 m1
Test volume
15 ml
Incubator#
1
Light
161L/8dk
Initial Temp °C
Selenastrum
0.05 ml
YAT
0.05 ml
Test method
EPA e21-R-02-013d092
ini
D=Dead N/A -Lost or not used
Page 4 of 6

ETT�
PO Box 16414, Greemrille, SC 29606-7414
[phone]. [phone]
Shipping Address: 4 Craftsman Cl, Greer, SC 29650
w .et lab.my
CHAIN OF CUSTODY RECORD
Page of,
Client: h V Tn W Ni-K, Q
Q I`
Program
Containers
Presenarlvc
Parameters
Facility: (t,s S. VK4S �rp.
Whole Emoent To:ieiry•
l
O
—
❑
State: NPDES k:
Acuic
Chrenlc
T.1 o,eaetsae
c
r
(Composite oily)
(Grab or Composite)
o
Sign, and Print below
3
a
Htt.
E
c
.2
m
vt
? — o
'�
'_
<
U
111e dosed IIIIe
E
Efl'J.91U
�,ai•OHSAMPLE
_
1D
ea pe bes� D.re Tlme
s mple eel�eo Dam Time
ollmtd by
U ti
m
a d
U >
— s, op
<
<
U
U
C 0
c
'm _ u
_ 3
Cnemleal Analysis & Otner
b I
6
ti�
---
-- --11 ----e.
WIN
�
19�52SA
&
-------------
------------
Special Instructions:
^^L1M y-y-23 OD FA t-i-�I -Z'�
_
�L was v td Li G eA- +11:Is
Sample Custody Transfer Record
r
Secure
Receipt
Sample
Date Time Re' uishe /Or i lion Received v/Oreanization
Area
Temp eC
Preserved?
o1Sto
z
D
,�:I
�f�Lt /z3
to i
Composite samples m t be collected over a 24 hour period. Sample temperature during collection end tratlsport must be between For to>;icity testing rile sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours. Equal voles 0.0 and 6.0 °C. Samples must not be frozen. Use hater ice in scaled bags. ofsample collection (completion ofcomposite sample).
or at minimum 1 sample every 4 hours over 24 hours. Sample may not be used after 72 hours front sample collection.
Plow Proportional: As oar instructions in NPDES hermit.
Page 5 of 6

ETT114-
PO Boa IU14, Greenvft, SC 296W7414
[phone], (81" 891-2325
Shipping Address: 4 CWsman Ct, Greer, SC 29650
wrrN.ettlaharg
CHAIN OF CUSTODY RECORD
Page - of
Client:
C' 1/!lfTK
Program
Containers
Presen•ath•c
Parameters
Facility: L� S - v K�
`/
W + 1
N'hotc Emuent T-idy
0
State: NPDES n:
Aeule
Chmak
Tan Orgo.!,—
O
U(Composite
only)
(Grab or Composite)
o
«
U
�-
U
j
t✓
Sign, and Print below
3 =
e
c
1-tuxes
z-xa
C
the dotted line
€
E C
v "
E
„wOH
u
r
2
o`
ti
o
r-Zane
u
= ''•
= % =
ut
SAMPLE ID
t?
Campue5unoar Times
srmpeCobNmDam Time
Cal edby
U H
Z
x a
O >
— 4.p�
¢
<
U
U
(J
F
m = U
F 3
ChemleatAnalyslsaOthar
I
`i L
�'io
— -- fixL4 55
C ii�X
N k
-------------
-------------
-------------
Special Insruclions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time 'n uish B / Organization vedB rganizali o
Tern 'C
Preserved?
A 0y
2
t77-
COMPOSITE SAMPL/M ROCEDURES
TEMPERATURE MOM/TOR/MG PROCEDURES
HOLD TIME PROCEDURES
Composite samples oncollected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used tlithin 36 hows
Time Proportional: I sample each hour for 24 hours. Equal volui
0.0 and 6.0'C. Samples must not be frozen. Use later ice in sealed bags.
of sample collection (completion of composite sample).
or al minimum I sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in NPDES Permit.
Page 6 of 6

Ecnjo ranmcnta , Ina.
P.O. Box 1 Ea114, Greenville, SC 29606
(EC4) 377-E�42 , FA>( [phone]
4 Crailsman Cuurl, Greer, 5C 2N50
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 12-Jul-23
Laboratory ID #: T65191
Test Reviewed and Approved By:
Robert W. Kelley, Ph.D.
President
Certification #E87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Certification Number E87819
Florida Dept, of Health. Included results pertain only to provided samples.
Page 1 of 6
Ainy McMahan
QA/QC Review
SCDHEC Certification #23104
NCDENR Certification # 022

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 01-Aug-23
Facility: CHARLOTTE WATER Lee S. Dukes NPDES# NCO084387 Pipe # 001 County. Mecklenburg
Laboratory Performing Test: 1=1 Comments
X Z�)av-1�179 aan� 8-4-2023
Signature of Operator in Responsible Charge
X
Signature of Laboratory Supervisor
MAIL ORIGINAL TO
CONTROL ORGANISMS
# Young Produced
Adult (L)ive (D)ead
Effluent %
TREATMENT 2 ORGANISMS
# Young Produced
Adult (L)ive (D)ead
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Chronic Test Results
Calculated t=
Critical Value=
1 2 3 4 5 6 7 8 9 10 11 12 % Reduction=
21
22
20
22
18
20
19
20
20
19
23
19
L
L
L
L
L
L
L
L
L
L
L
L
1 9 3 d 5 R 7 A Q 1n 11 17
22
18
22
22
18
19
19
22
20
21
19
21
L
L
L
L
L
L
L
L
L
L
L
L
pH 1st sample 1st sample 2nd sample
Control 7.8 7.7 7.5 7.9 7.8 8.2
Treatment 2 7.7 7.8 7.6 8.0 7.8 8.2
start end start end start end
D.O. lstsam le lst sam le 2nd sam le
Control
Treatment 2
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
LC50 = %
95 % Confidence Limits
Oraanism Tested
Method of Determination
Average Probst
an Karber ROther
Ceriodaohnia dubia
to This for Either Test
to !!Start) Date
1 11-Jul-23
Sample 1 X
Sample 2 X
Hardness (mg/L)
Spec. Cond. (pmhos)
Chlorine (mg/L)
nple Temp. at receipt ('C)
0
2.508
0.0 %
0%
Control
20.3
Control
0%
-eatment2
20.3
Treatment:
onlrol CV
7.3%
PASS FAIL
%3rd Brood
100%
Test Start Date
12-Jul-23
Samole 2 13-Jul-23
1st 2nd
Tox Tox
Dilution Sample Sample
82.0
314 88 84
<0.05 <0.05
1.1 1.3
Mortality start/end slarUend
Control
High Conc.
pH D.O.
91SIA191T, refivL\dt
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
CHARLOTTE WATER
NPDEs# NC0084387
[acii
ple ID: Lee S. Dukes
ETT# T65191
Date: 12-Jul-23
rato :
Certification #: NCO22
Exp. Date: l l/l/2023
Survival Data
7 Day Survival
Test Used: FISHERS TEST
Control 100%
Test Statistic: P= 1.000
Effluent 100%
Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test for Normality
Mean young/female
Std. Dev.
Test Used: Shapiro -Wilke Test:
Control 20.3
1.48
Effluent 20.3
1.60
W:
0.917
The data are nor dls) r"ed.
0.884
Analysisfor Differences in Reproduction
Test for Homogeneity of Variance
Test Used: Equal Variance t Test.
Test Used: F Test
Calculated t=
0.00
F=
1.16
Critical Value--
2.51
Critical Value=
5.32
The data are homogeneous in variance
PASS: The effluent is
not chronically toxic.
Page 3 of 6

Control Mortality and Reproduction by Test Day
2 3 4 5 6 7 8 Total
6-28
1
1
1
+4+7
1
10
6-28
6-29
2
+3+7
12
3
+4+6
10
6-29
4
+4+8
10
6-29
5
+3+6
9
6-29
6
+4+7
9
6-30
7
+3+6
10
6-30
8
+3+6
11
6-30
9
+4+6
10
6-30
10
+3+7
9
7-5
11
+4+8
11
6 7-5
72
+4+6
9
15
16
M5 6-28
37 % Effluent Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
1
+4+7
11
M8 6-28
2
+3+6
9
K6 6-29
3
+4+8
10
K7 6-29
4
+4+8
10
N5 6-29
5
+3+6
9
07 6-29
6
+3+7
9
E6 6-30
7
+4+6
s
D7 6-30
8
+4+7
11
V6 6-30
9
+3+7
10
X8 6-30
10
+4+7
10
113 7-5
11
+3+6
10
KK6 7-5
12
+4+8
9
0
13
N/A
0
14
N/A
0
15
N/A
0
16
N/A
0
17
N/A
0
18
N/A
0
19
N/A
0
20
N/A
renew Initial
AM
JG
End Date
fed by
JG
AM
AM
JC
JG
JC
19-Jul-23
time fed & renew
10.12 AM
01:10 PM
06:16 AM
05.33 AM
10:21 AM
07:36 AM
09:51 AM A
New eff. •C
25.0
24.8
Old lemp.'C
25.6
25.5
25.5
New C..t•C 24.7
24.81
24.6
21
22
20
22
18
20
19
20
20
19
23
19
0
0
0
0
0
0
0
0
22
18
22
22
18
19
19
22
20
z1
19
21
0
0
0
0
0
0
0
0
Lab#
T65191
Client
CHARLOTTE WAT
Sample ID
Lee S. Dukes
NPDES#
NCO084387
County
Mecklenburg
Month
7
Start & fed Date
12-Jul-23
Start & fed Time
12:30 PM
Started & fed By
JG
Test Organism
Ceriodaphnia dubia
Neo. born date
11-Jul-23
Neo. born time
1700-2200
Test Type
NCCPF
Dilution Water
MHSF
Units for Conc.
%
IWC
37
%3rd BROOD
Test vessels
Test volume
30 ml
15 ml
Incubator#
1
Light
16lt/8dk
Initial Temp °C
Selenastrum
0.05 ml
YAT
0.05 ml
Test method
PPA e21-R-02-013:1002
ini tem
D=Dead
Comments
Page 4 of 6

ETT'
PO Baz 16414, Greenville, SC 29SW7414
[phone], [phone]
Shipping Address:4 CraBsman Cl. Greer, SC 29&%
www.ett[sh.org
CHAIN OF CUSTODY RECORD
Page of_L
Client:
Program
Containers
Preservative
Parameters
Facility:
Q
—
c E
` o�
U h
o
--
2 is
E r=
o
z
u
c
v "
o
w a
c
_=
U >
t-xtt
3-x 40301i
s-rMa
— d p
whole Emomt Ta:idry
ce
- 3
p
Chemical Analysis & Other
p
to
�
O
<
State: G NPDESN:
Acme
Chrome
Tot Organism
SAMPLE ID
_
U
V
(Composite only)
Cwnpmlie sun Due Time
(Grab or Composite)
Soap a [me 4m a.m Ttme
Sign, and Print below
the doped line
Corrected by
=
_
<
Li
U
o
<
a
U
U
°o
U
e
' '—
�
U
o
_ '—
2 2_
O = ii
=lZR
H - U
v
11�yj
PIA
I
I
I
b511IA
-------------
-------------
-------------
-------------
Special Instructions:
Sample Custody Transfer Record
Date Time RelinggishedByOrganization Received0 /Organization
Secure
Area
Receipt
Tern aC
Sample
Prescrvcd?
COKeI
/
O
Co
-ar/0� 1/t3
l 2 3122to
CST of-&-1° r
4r3S
<.o
Composite samples mu a collected over a 24 hour period. Sample temperature during collection and transport must be between For toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours. Equal voles 0.0 and 6.0 eC. Samples must not be frozen. Use water ice in sealed bogs. of sample collection (completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours. Sample may not be used after 72 hours from sample collection.
Flow Proportional: As Der instructions in NPDES oemlit.
Page 5 of 6

ETT
PO Box 16414, Gnenvme, SC 29808-7414
[phone], [phone]
Shi
pping Address: 4 CraBsman Ct. Greer, SC 29850
rnrrztUebary
CHAIN OF CUSTODY RECORD
Page L off_
Client:
i'a M& ,F—T
Program
I Containers
Preservative
Parameters
Facility: l✓�� 5. D d 1( f S W'f
wh.le emaem Te:IAq
o
o
o
State: C, NPDES p: '1•
Ac.te
Chrome
I Text ora..i:.r.
_
G
'
u
(Composite only)
(Grab or Composhe)
<
r:`
c
CCZj
e
t•wsa
o_
'
c E
g
yr
a
Sign, and Print below
=
v
=
v
:-tict.
f
e
f
e o
1iJ
=
<
the Boned line
€
E r
.Y
o
.-wort
to2
'
2
°
c
e
SAMPLE ID
o
C.-oa,. sw D.m Times
^� i. C.n.m.. om Tlme
Collected by
U y
y Z
a a
0 >
= S. In A.
b- 0a
<
<
U
U
U C7
s� E
sn 2 U
F 3
E Chemical Analysis & Other
11�Xj
1 1W
65I91B
-------------
--------------
-------------
pecial Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Re •n uished / O i2adon Received By / Organization
Area
Temp°C
Preserved?
C
'
W
33 23
miO
OSITE SAMPLING ROCEDURES
TEA&ER4ruREMONIMRING PROCEDURES V
HOLD TIME PROCEDURES
Composite samples mus a collected over a 24 Hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volui
0.0 and 6.0'C. Samples must not be frozen. Use water ice in sealed bags.
ofsample collection (completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours.
Sample may not be used after'P_ hours from sample collection.
glow proportional: As per instructions in NPDES permit.
Page 6 of 6

CflV rnnmcnta ,Inc.
P.O. Box 4 E-414, Creenv Ile, SC 29606
(EG4) 077—G +42 . FAX [phone]-G930
4 Craftsman Cwrt, Greer, SC 29050
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 04-Oct-23
Laboratory ID #: T65822
Test Reviewed and Approved By:
d��hert �elle�
Robert W. Kelley, Ph.D.
President
Certification #E87819
Test results presented in this report conform to all requirements of
Amy McMahan
QA/QC Review
SCDHEC Certification #23104
NELAC, conducted under NELAC Certification Number E87819
Florida Dept of Health Included results pertain only to provided samples.
Page 1 of 6
NCDENR Certification # 022

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 23-Oct-23
Facility-, CHARLOTTE WATER Lee S. Dukes NPDES# NCO084387 Pipe # 001 County. Mecklenburg
Laboratory Performing Test: 7� -/ Comments
X f/aU�G7l
Signature of Operator in Responsible Charge
X d�ohert �e��ec�
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t= 0.9682
Critical Value= 2.508
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Reduction= 2.8 %
# Young Produced
Adult (L)ive (D)ead
Effluent %
TREATMENT 2 ORGANISMS
# Young Produced
Adult (L)ive (D)ead
20
19
21
22
20
19
21
21
19
21
22
21
L
L
L
L
L
L
L
L
L
L
L
L
1 d S R 7 R 9 1n 11 19
21
21 1
19
18
19
23
17
22
20
21
20
18
L
L
L
L
L
L
L
L
L
L
L
L
Complete This for Either Test
Collect on fStarb Date
pH tsl sample lslsample 2nd sample Sample 03-Oct-23
Control 7.8 7.8 7.7 7.9 7.6 8.3 Sample Tvne (Duration)
Treatment 2 7.7 7.7 7.7 7.9 7.7 8.1 Gab con
Sample 1 X
Sample 2 X
start end start end start end
D.O. lstsam le lstsam le 2nd sam le Hardness(mg/L)
Control 8.5 8.6 8.0 8.8 8.4 9.3 Spec. Cond.(pmhos)
Treatment 2 8.8 8.7 8.8 8.8 9.0 9.6 Chlorine (mg/L)
Sample Temp. at receipt (°C)
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
LC50 = %
95% Confidence Limits
%
Organism Tested
0%
Control
20.5
Control
0%
Treatment 2
19.9
Treatment 2
Control CV
5.3%
PASS FAIL
%3rd Brood
100%
X
Test Start Date
04-Oct-23
Sample 2 05-Oct-23
1st 2nd
Tox Tox
Dilution Sample Sample
86.0
320 89 94
0.08 0.09
2.5 1.5
Mortality, starVend start/end
Method of Determination Control
Average Problt High Conc.
an Karber ROther pH D.O.
is dubia
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facility: CHARLOTTE WATER
NPDES# NC0084387
Sample ID: Lee S. Dukes
ETT# T65822
Date: 04-0ct-23
Laboratory:
Certification#: NCO22
I Exp. Date: ll/l/2023
Survival Data
7 Day Survival
Test Used: FISHERS TEST
Control 100%
Test Statistic: P= 1.000
Effluent 100%
Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test for Normality
Mean young/female
Std. Dev.
Test Used: Shapiro-Wilks Test:
Control
20.5 1.09
Effluent
19.9 1.78
W:
0.979
Critical Value:
0.884
Analysisfor Differences in Reproduction
Test for Homogeneity of Variance
Test Used: Equal Variance t Test.
Test Used: F Test
Calculated t=
0.97
F=
2.69
Critical Value—
2.51
Critical Value--
5.32
The data are homogeneous in variance
PASS: The effluent is not chronically toxic.
Page 3 of 6

source
#/re =l
Control Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
J10 9-21
A
+4+6
10
20
18 9-21
09 9-21
A
+3+7
9
19
A
4
+6+11
21
P3 9-21
A
+4+6
10
22
Al 9-22
B
+4+6
10
20
F7 9-22
GG2 9-27
B
+4+6
9
19
B
1
1
+3+7
1
11
1
21
JJ1 9-27
B
+3+6
12
21
112 9-27
C
3
+7+9
19
HH3 9-27
C
+3+7
11
21
H8 9-21
C
4
+8+10
22
6 9-22
C
4
+6+11
21
D
N/A
0
D
N/A
1
0
D
N/A
1
0
D
N/A
I
I
I
I
1
0
E
N/A I I I I I 1
0
J10 9-21
37 % Effluent Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
A
+4+7
10
21
18 9-21
A
+4+6
11
21
Q9 9-21
A
+3+5
11
19
P3 9-21
A
+3+5
10
18
Al 9-22
B
+3+7
9
19
F7 9-22
GG2 9-27
B
+4+7
12
23
B
+3+6
8
17
JJ19-27
B
4
+9+9
22
112 9-27
HH3 9-27
H8 9-21
C
+3+5
12
20
c
+4+7
10
21
c
4
+6+10
20
V6 9-22
C
+3+6
9
18
0
0
D
N/A
0
D
N/A
0
0
D
N/A
0
0
D
N/A
0
0
E
N/A
0
0
E
N/A
0
0
E
N/A
0
O
E
N/A
0
fed by
jr
JZ
I JC
JZ
I AM
JC
11-QCt-23
time fed & renew
06:53 AM
02:16 PM
06:30 AM
07:30 AM
11:12 AM
07:45 AM
10:56 AM A
Newerf.'C
24.8
25.0
Old t—p.'C
25.2
24.7
24.9
New Conl'c 1 24.7
24.5
24.6
Lab#
T65822
Client
CHARLOTTE WAT
Sample ID
Lee S. Dukes
NPDES#
NCO084387
County
Mecklenbur
Month
10
Start & fed Date
04-Oc1-23
Start & fed Time
01:30 PM
Started & fed B
JG
Test Organism
Cerioda hnia dubia
Neo. born date
03-Oct-23
Neo. born time
1700-2200
TestType
NCAPF
Dilution Water
MHSF
Units for Conc.
IWC
37
%3rd BROOD
Test vessels
30 ml
Test volume
15 ml
Incubator #
1
Light
161t/8dk
Initial Temp 'C
Selenastrum
0.05 ml
YAT
0.05 ml
Test method
UA621-R-02-013:1002
(Control ini
N/A -Lost or not
Page 4 of 6

ETT _
N— .
PO aoz 16414. CweemWe, SC 29WB-74 44
(e54)877-6942, [phone]
Shipping Adorers: 4 Craftsman Ct, Greer, SC 296M
www.ettleb.org
CHAIN OF CUSTODY RECORD
Page -A_ of=-]
Client:
Program
Containers
Prescnative
Parameters
Facility: •emu 4e
whole t muerrl T-kk
p
o
State: rJC, NPDCS#: 11I.M.
Aewe
Cbraaic
T.t Orz..km
_
Li
c
-
p
U(Composite
only)
(Grab or Composite)
C
o
c
= U
U
1-1
O
Sign, and Print below
3>
r
U
1-HS042- HCL
c
a
'm
die doped line
.E
u
-
E
c E
3•HNO3
,.N,oH
2
.o
E
SAMPLEID
t3
c•mpa,ik sun p.m Time
5Bmpk C•nkioa r- Tima
Cdl—d by
U yr
m Z
a a
U >
= e Or
< ¢
U
U
U t]
� f
H TU
i= 3
i Chemical Analyrls80tner
«3.13
-------------
Special Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Relinquished B / Organization Rs5ELng B / Or nation
Area
Temp eC
Preserved?
U
1004
10-03- j
Gowt ,;,
DQ323
IIN
a,
I+s
COMPOSITE SAMPLlN PROCEDURES
TEMPERATURE MON/TORlNO PROCEDURES
HOLD TIdfE PROCEDURES
Composite samples rotas be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing die sample must first be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volu
0.0 and 6.0 *C. Samples must not be frozen. Use water ice in sealed bogs.
of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in NPDES pennit.
Page 5 of-6-

ETT
VIM
�I en
~PO 8oa 16414, Greenvote, SC 291306 414
(854) 877-&%2, IWO) 591-2325
SNpptng Address: 4 Craftsman Ct. Greer, SC 29M
anvn.ettleh.arg
CHAIN OF CUSTODY RECORD
Page -A— of_l
Client:
L zTn5 WNOZK
Program
I Containers
Prts—ative
Parameters
Facility:
S DUKC
%%'hot. Effl. v T-min•
p
o
o
State: NPDES M: p
A.C.
chronic
Tetone n
_
6.-
U
p
U(Compmim
only)
(Gmb or Canposilo)
<
it,—
U
_
o c
a
p
and Print below
3
2 m
E r
c
u=
k
i- uCt -KNOa
1
u
u
m
y
E
41
<
o
the doped line
€
�, on
>;
e
e
SAMPLE ID
o_
oq•ml�e wn our Tima
Time
Semple La[kcGoe Du. me
Cnllead by
6.0Lh,
¢
<
U
U
U d
32
chemical Analysis 8 Other
I RLL-L-
N,
�ssaae
-------------
-------------
-------------
LL
M-1
Special Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Relinquished B / Orzanization Received B / 0ozamnition
Area
Temp eC
Preserved?
S
111
t mct a-'c
O J
0
4
Gp
l0 5 23
1062
Il yi
C. W C IN
a
Tr -
Co OSITE SAMPLI PROCEDURES
T IPERATURE MONITORING PROCEDURES
HOLD TIME PROCEDURES
Composite samples m t be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing die sample most first be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volu
0.0 and 6.0 eC. Samples must not be frozen. Use waler ice in sealed bags.
of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in NPDES permit.
Page 6 of 6

ETT
environmental, inc.
P.O. Box 16414, Greenville, SC 29606
(864) 877 - 6942
4 Craftsman Court, Greer, SC 29650
Ceriodaphnia dubia Chronic Pass/Fail Toxicity Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 03-Jan-24
Laboratory ID #: T66479
Test Reviewed and Approved By:
Robert 'qet!leL4
Robert W. Kelley, Ph.D.
Laboratory Manager
9g ® Certification #E87819
Text results presented in this report conform to all requirements of
NELAC, conducted under NELAC Certification Number E87919
Florida Dept. orHealth. Included results pertain only to provided samples.
Amy McMahan
QA/QC Review
SCDHEC Certification #23104
NCDENR Certification # 022
Page 1 of 6

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 12-Jan-24
Facility: CHARLOTTE WATER Lee S. Dukes NPDES # NCO084387 Pipe # 001 county: Mecklenburg
Laboratory Performing Test: Cemmenn
Signature of Operator in Responsible Charge
x
Signature of Laboratory Supervisor
MAIL ORIGINAL TO
Environmental sclences Branch Div. of
Water Quality N.C.
DENR
1621 Mail Service Center Raleigh, North
Carolina 27699-1621
North Carolina Ceriodaphnia Chronic Pass/Fail
CONTROL ORGANISMS
Reproduction
12
Toxicity
]
Test
5
a
7
It
9
10
11
12
a Voung Produced
21
24
25
23
25
24
22
21
24
23
23
23
Adun (L11ve to lead
L
L
L
L
L
L
L
L
L
L
L
L
Effluent % 37.0%
TREATMENT 2 ORGANISMS
1
2
]
e
S
6
]
a
9
1p
11
12
ed
ELlpl!U.)—d
22
21
22
24
20
25
24
24
24
23
23
23
L
L
L
L
L
L
L
L
L
L
L
L
start end start end start end
pH 1st sa-78 l1s7t s.7am le 8.1 2nd .7sam le
Control 77 7.9
Treatment 8.3
start end start end start end
D.O. 1st saD7.8
1st sam le 2nd sample
Control 8.2
Treatment 2 8.3
LC50/Acute Toxicity Test
IM orla liry exp re seed ae %, co mbining repawlee)
LC50 = % Method of Determination
95 A Conndence Llmlts ving Average Probit
% % Spearman Barber Other
Organism Tested Ceriodaphnia dubia
DEM Form AT-1
I. Thle fer Either Taal
:tion (Start) Date
le 1 02-Jan-24
Tvue fD walb�l
Sample 1
Sample 2
mmm
©MM!
©mm
Hardness (mg/L)
Spec. Cond. (pmhos)
Chlorine (mg/L)
Imp. at receipt PC
onic Test Results
culated t = 0.44
ical Value= 2.508
teduction= 1.1
Aortality Avg. Reprod.
0% 23.2
;ontrol Control
0 % 22.9
itment 2 Treatment 2
rol CV
5.8%
3rd Brood PASS FAIL
100 % j X
Test Start Date
03-Jan-24
Sample 2 04-Jan-24
1st 2nd
Tox Tox
Dilution Sample Sample
83.7
317 88 93
<0.05 0.07
1.61 1.5
ME . =0
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facili : CHARLOTTE WATER
NPDES# NC0084387
Sample ID: Lee S. Dukes
ETT# T66479 Date: 03-Jan-24
Laborator
Certification #: NCO22
Ex . Date: 11/1/2024
Survival Data
7 Day Survival
Test Used: FISHERS TEST
Control 100%
Test Statistic: P= 1.000
Effluent 100%
Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Rasa Data
Testfor Normality
Mean young/female Std. Dev.
Test Used: Shapiro -Wilke Test:
Control 23.2
1.34
Effluent 22.9
1.44
W : 0.946
Critical Value: 0.884
The data are normally distributed.
Analysisfor Differences in Reproduction
Test for Homogeneity of Variance
Test Used: Equal Variance t Test
Test Used: F Test
Calculated t =
0.44
F= 1.17
Critical Value=
2.51
Critical Value= 4.40
The data are homogeneous in variance
PASS
Reproduction is not reduced in the eftlncut
Page 3 of 6

Control Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
source
rep
D2 12-22
1
+3+7
11
21
K1 12-21
2
+5+7
12
24
66456 CO
3
+3+9
13
25
J 1 12-21
4
+4+7
12
23
66456 CO
5
+4+8
13
25
L8 12-21
6
+5+7
12
24
E4 12-22
7
+3+8
1
11
22
66461 CO
8
+4+7
10
21
G2 12-21
9
+4+7
13
24
J10 12-21
10
+4+7
12
23
K10 12-21
11
+3+8
12
23
66461 CO
12
+3+8
121
23
13
N/A
14
N/A
15
N/A
16
N/A
17
N/A
18
N/A
19
N/A
Mean
20
N/A
23.2
37 % Effluent Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
D 2 12-22
1
+3+7
12
22
K1 12-2
IJ1
2
+4+7
10
21
66456 C
3
+4+6
12
22
12-2
4
+4+7
13
24
66456 C
5
+3+6
11
20
L8 12-2
6
+4+7
14
25
E412-2
7
+3+8
13
24
66461 C
8
+4+8
12
24
G212-
9
+6+7
11
24
J10 12-1.
10
+4+7
12
23
K10 12-2
11
+4+7
12
23
66461 C
12
+5+7
11
23
0
13
N/A
0
14
N/A
0
15
N/A
0
16
N/A
0
17
N/A
0
18
N/A
0
19
N/A
Mean
01
20
N/A
22.9
renew Initial
JZ
JC
End Date
fed by
AM
JZ
JZ
JC
JC
JC
10-Jan-24
time fed & renew
11:31 AM
11:36 AM
07:30 AM
06.30 AM
11:01 AM
01.10 AM
10:36 AM AM
Newert.'C 24.6
24.8
25.5
Old temp.'C
25.2
25.1
24.9
New Con1 ° 24.61
1 24.51
1 24.6
Lab#
479
Client
ARLOTTE WAT
Sample ID
S. Dukes
NPDES#
[NCO084387
Count
cklenburMonth
Start d fed Date
03-Jan-24
Start & fed Time
01:30 PM
Started & fed By
JC
Test Organists
Ceriodaphnia dubia
Neo. born date
02-Jan-23
Neo. born time
1710-2200
TestT pe
NCCPF
Dilution Water
MHSF 12/20/23E
Units for Conc.
IWC
37
%3rd BROOD
Test vessels
30 ml
Test volume
15 ml
incubator #
1
Light
161U8dk
Initial Temp 'C
24.8
Selenastrum
0.05 ml
YAT
0.05 ml
Test method
EPA a21-R-o3-o13:1oo2
Comments
Page 4 of 6

CHAIN OF CUSTODY RECORD
PO Box 18414, Greenville, SC 29808-7414
[phone], [phone] Fax:[phone]
Shipping Address: 4 Craftsman Cl, Greer, SC 20650
Page of
Client:
A-11 L,
Program
Comniacrs
Prese"otive
Parameters
Facility:
S
9 Dylees,
n'nem emaenr rarmlty
—
o
00
State: G NPDES 1S: d ff
Acme
Chm.lcl
T.t Orqa
.
o
o
U((Composite
only)
(Grob or C—posile)
Q
o
u
L
p
U
_
U
c
Z
n
Sin, and Print below
g
3
m
=
I-F450+
! HCL
y
u,
"
a
�E
—
Ole dotted line
E
_E
u .=
_
3•HNo3
._
--
— —
SAMPLE ID
o
c�pvaesmn oor Times
uvpk Cellmtov ovtc Time
Collected by
U rn
y Z
a o
V >
$ s=znne
5=per
<
<
U
U (.1
f]
L
vs — U
i—+
Chemical Analysis 6 Other
-------------
r �
�b�tzq�
001
_
��
AM
-------------
-------------
-------------
pecial Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Relit uislied B / Organization —Received liv / tion
Area
Tem eC
Preserved?
?�y
l
w
;0 CU 1�
%Yl�k a ylC 'wt
12LH
E
W3
' , G WV
rLr
ti E?T
V% So5
k • b
COMPOSITE SAd4PLIN R0CEDIQES
TEXIPERziTURE MON/TOR4NG PROCEDURES
HOLD TIME PROCEDURES
Composite samples mu be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used within 36 hours
Time Proportional: 1 sample each (tour for 24 hours. Equal volu
0.0 and 6.0 °C. Samples must not be frozen. Use hater ice in sealed bags.
of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used after 72 Hours from sample collection.
Flow Proportional: As per instructions in NPDES permit.

ETT
111V111 110
PO Box 15414, Gte—A., SC 298I18-7414
[phone]• [phone] Fi n:(8(14) 877 6938
Shipping Address:4 Craftsman Cl, Greer, SC 296$0
CHAIN OF CUSTODY RECORD
Client �t
Program
CenWlaers
Preservative
Parameters
Facility:`
�G .iti t
lvbeieemeeat re:tip•
m
4
State: IVC, NPDES#: e—
seine
Chroni
Tut o -1—
0
�
E
(Composite only)
(Gnb or Composite)
`
o
u
—
u
U
i
—
c
E
z
O
a
Sign, and Print below
3
e m
c
u
-HLl
tswot
—
a
die dotted line
5 €
E
.
,.rsoH
—
_`e
`e
o
o
e
SAMPLE ID
t?
cumpmh, Stan Dete Time
seoryk Cmeer.a Dme Tlma
Coiledcd b
U y
n Z
a a
O >
— s. ome
<
<
U
U
U
n U
[- 3
f Chemical Anelyeis L Other
i-y-,Vy
071&-_
----.�
kJYJ
Jw,-
I -41A
Y
lab'��gB
-------------
-------------
-------------
pecial Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Relinquished By/ Or ization Received B Ormnizatjori
Arcs
Tnnp °C
Preserved?
D
L-2-q
K a
11
5
OMPOSITE SAMPUN ROCEDURES
T£MPE TURF MONITORING PROCEDURES
H TIME PROCEDURES
Composite samples m e. collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing die sample must first be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volu
0.0 and 6.0 oC. Samples must not be frozen. Use water ice in sealed bags.
of sample collection (completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in NPDES permit.

ETeuvwmTentainc. (9G4) 877.6942 , FAX [phone]
P.O, Box 16414, Greenville, 5C 29606 4 Craftsman Court, Greer, SC 29650
Ceriodaphnia dubia Chronic Pass/Fail Toxicity Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 03-Apr-24
Laboratory ID #: T67205
Test Reviewed and Approved By:
Robeet �ellee�
Robert W. Kelley, Ph.D.
Laboratory Manager
0
Certification #E87819
Test results presented in this report mote= to all requirements of
NELAC, conducted under NELAC Certification Number B87319
Florida Dept. of with. Included results Fermin only to provided samples.
Amy McMahan
QA/QC Review
SCDHEC Certification #23104
NCDENR Certification # 022
Page 1 of 6

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 15-Apr-24
Facility: CHARLOTTE WATER Lee S. Dukes NPDES # NCO084387 Pipe # 001 County: Mecklenburg
Laboratory Performing Test: c.mm.au
X (/7j�� LG( '` Z // v
Signature of Operator In Responsible Charge
ert �e �ee� X
Signature of Laboratory Supervisor
MAIL ORIGINAL TO
Environmental Sclences Branch DI, of
Water Duality N.C.
DENR
1621 Mail Service Center Raleigh, North
Carolina 27699-1621
CONTROL ORGANISMS
1
2
3
S
6
7
a
9
10
11
12
x Young Produced
21
22
19
19
23
22
20
20
21
18
22 1
21
Aden (L)- (D)ead
L
L
L
L
L
L
L
L
L
L
L
L
Effluent % 37.0
TREATMENT 2 ORGANISMS
1
2
3
4
5
6
7
6
9
10
11
12
N Young Produced
21
23
17
20
21
19
11
19
22
21
20
19
Add1 (t)M. (o).ad
L
L
L
L
L
L
L
L
L
L
L
L
start end start end start end
pH tstsaR8.3
1st sam le 2nd sam le
Control F7.8Treatment 2
start end start end start end
D.O. Ist saD7.6
1st sam le 2nd sample
Control E8802
Treatment 2 7.6 7.5 9.1 7.9
(M.r 10,..pr.... d.e%. combining repllcales)
LC50 = %
95% Confidence LIm Oe
Organism Tested
DEM Form AT-1
Method of Determination
1—rg Avon,. Probil EJ
Spe,rm,n h,m,r Other
Ceriodaphnia dubia
Thl• for Either Te,l
Dn (Start) Date
1 02-Apr-24
Sample 1
Sample 2
Chronic Test Results
Rank Sum = 132.50
Critical Value= 109
% Reduction= 6.0%
% Mortality Avg. Reprod.
0% 20.7
Control Control
0% 19.4
Treatment 2 Treatment 2
Control CV
7.2%
3rd Brood PASS FAIL
100 % X7
Test Start Date
03-Apr-24
Samole 2 04-Apr-24
mmmi
mmm
NOW
Hardness mg/L)
Spec. Cond. (pmhos)
Chlorine (mg/L)
MD, at receipt ("CI
1st 2nd
Tax Tax
Dilution Sample Sample
84.0
319 87 85
<0.05 <0.05
2.21 3.1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facility: CHARLOTTE WATER
NPDES# NC0084387
Sample ID: Lee S. Dukes
ETT# T67205 Date: 03-Apr-24
La oratory:
Certification #: NCO22 I
Exp. Date: 11/1/2024
Survival Data
7 Day Survival Test Used: FISHERS TEST
Control 100% Test Statistic: P= 1.000
a
Effluent 100% Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
�3
Rain Data
Test for Normality
Mean young/female
Std. Dev.
Test Used: Shapiro-Wilks Test:
Control 20.7
1.50
Effluent 19.4
3.09
W: 0.847
Critical Value: 0.884
The data are not normally distributed
Analysis for Differences in Reproduction
Test for Homogeneity of Variance
Test Used: Rank Sum
Test Used: F Test
Rank Sum =
132.50
F= 4.25
Critical Value=
109.00
Critical Value= 4.40
The data are homogeneous in variance
PASS
Reproduction is not reduced in the ettluent
Page 3 of 6

Control Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
source
rep
M8 3-21
1
+3+8
10
21
KK3 3-27
2
+4+7
11
22
KK4 3-27
3
+4+7
8
19
GG4 3-27
4
+3+7
9
19
N5 3-21
5
+4+7
12
23
Q 1 3-21
6
+5+6
11
22
S4 3-25
7
+3+6
1
11
20
S3 3-25
8
4
+6+10
20
D2 3-25
9
+2+8
11
21
C10 3-25
10
+3+7
a
18
K4 3-21
11
+4+7
11
22
G9 3-21
12
3
+7+11
21
13
N/A
14
N/A
15
N/A
16
N/A
17
N/A
18
N/A
19
N/A
Mean
20
N/A
20.7
37 % Effluent Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
M8 3-21
1
+3+7
11
21
KK3 3-2
2
+3+8
12
23
KK4 3
3
+3+6
8
17
GG4 3-2
a
+3+6
11
20
N5 3-21
5
+3+7
11
21
Q1 3-21
6
3
+6+10
19
S4 3-25
7
41
7
11
S3 3-25
8
+3+7
9
19
D2 3-25
9
+4+6
12
22
C10 3-25
10
+3+6
12
21
K4 3-21
11
4
+5+11
20
G9 3-21
12
4
+6+9
19
0
13
N/A
0
14
N/A
0
15
N/A
0
16
N/A
0
17
N/A
0
18
N/A
0
19
N/A
Mean
01
20
N/A
19.4
renew Initial
JC
JC
End Date
fed by
JH JC JH JC JC JH
12 15 PM 12 21 PM 11:00 AM 06:00 AM 10:37 AM 11:00 AM
10-Apr-24
10 13 AM AM
fed & renew
eft.'c
24.7
25.0
emp.'C
LOW
25.2
25.3
25.8
coot •1
24.7
24.5
N/A -lost or not used
Page 4 of 6

�P
— Box 16414, Greenville, SC 29606-7414
[phone], [phone] Fa)c(664) 877 6938
Shipping Address: 4 Craftsman Ct, Greer, SC 29650
WWW-CT ENVIRONMEMTALXMM
CHAIN OF CUSTODY RECORD
Page _/—of—L I

CHAIN OF CUSTODY RECORD
PO Box 16414, GreenvHle, SC 29606-7414
[phone], (a0o) 891-2325 Falc(854) 877 5938
Shipping Address: 4 Craftsman Ct, Greer, SC 29650
W W W-7-rENV IROMMENTAL.COM
Page of
Client:
/
I—aile- 1,1a e
Program
Containers
Preservative
Parameters
Facility )'o
•� S J 7
t? f'i �C/"(f/Ce L(f I
Whole 1?mnnatTa.icih
Q
State: Z:— NPDES #: W L% ,' k /
Acute
Chronic
Test Organisms
d
0
p
c
c
(Composite only)
(Grab or Composite)
5
¢
o
a`�
CO
U
'c
o
U
'L
i?
c
�
to
Z
0U
Sign, and Print below
3
y
=
e
a
_
,�
o
u
I-HIS04
?- HCL
C
a
c
g
00
.�
m
-
c
Gtl
<
the dotted line
€
E
U
..2
E
s=HNos
a=N.OH
y
_
_
^
o
te
o
n
z
E
y
—
o
c+
Q
SAMPLE ID
U
Composite Start Date Time
Snmple Cnikcioo Date Time
Collected
U
$
rn
y
m
o
7-x
o
V-
U
o
>
$
5=ZnAc
6=Oilier
L
U
U
ci
b
r_
i
-
rn
2
U
F
3
Chemical Analysis &Other
LU
-------------
-------------
Special Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Relinquished By / Organization Received B / Organization
Area
Tem °C
Preserved?
2
Io:D3
a_ I' C
¢
IMs-
3
COMPOSITE SAMPLI PROCEDURES
TEA• ERATURE MONITORING PROCEDURES
HOLD TIME PROCEDURES
Composite samples in t be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volui
0.0 and 6.0 'C. Samples must not be frozen. Use water ice in sealed bags.
of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Plow Proportional: Asper instructions in NPDES ermit.

ET*nvrwmT0nt81nc. (1364) 877-6942 . FAX [phone]
P.O. Box 16414, Greenville, SC 29606 4 Craftsman Court, Greer, 5C 29650
Ceriodaphnia dubia Chronic Pass/Fail Toxicity Test
EPA-821-R-02-013 Method 1002
Client: CHARLOTTE WATER
Facility: Lee S. Dukes
NPDES #: NCO084387
Test Date: 24-Jul-24
Laboratory ID #: T67932
Test Reviewed and Approved By:
Rahert '(edit
Robert W. Kelley, Ph.D.
Laboratory Manager
o'-,
Certification 4E87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Certification Number E87819
Florida Dept. of Health. Included results pertain only to prodded samples.
Amy McMahan
QA/QC Review
SCDHEC Certification 423104
NCDENR Certification N 022
Page 1 of 6

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 07-Aug-24
Facility: CHARLOTTE WATER Lee S. Dukes NPDES # NCO084387 Pipe # 001 County: Mecklenburg
Laboratory Performing Test: Comments
X E/Lil�Ldi �G[�
Signature of Operator in Responsible Charge
X
,Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch Div. of
Water Quality N.C.
DENR
1621 Mail Service Center Raleigh, North
Carolina 27699-1621
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
1
2
3
4
5
6
7
8
9
10
11
12
%Young Pmduoed
23
23
22
21
19
24
22
21
22
21
21
23
Adult (L)iue (D)ead
L
L
L
L
L
L
L
L
L
L
L
L
Effluent % 37.0%
TREATMENT 2 ORGANISMS
1
2
3
4
5
6
7
8
9
10
11
12
tt Young Produced
21
21
23
21
19
23
21
21
22
22
19
21
Adult (L)we (D)ead
L
L
L
L
L
L
L
L
L
L
L
L
start end start end start end
pH 1st sample 1st sam le 2nd sam le
Control 7.7 8.3 7.7 8.2
Treatment 2 7.5 8.2 7.8 8.3
start end start end start end
D.O. 1st sample 1st saml2nd .5sam le
Control 6 8.9
8Treatment2 88.9
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
LC50 = % Method of Determination
95^h Confidence emirs Moving Average Problt
Spearman Kerber Other
Organism Tested Ceriodaphnia dubia
DEM Form AT-1
This for Ehher Test
in (Start) Date
1 23-Jul-24
Sample 1
Sample 2
Duration
EmbComp
Hardness (mg/L)
Spec. Cord. (Nmhos)
Chlorine (mg/1-)
TemD. at receipt ('C)
onic Test Results
culated t = 1.25
ical Value= 2.508
teduction= 3.1
Aortality Avg. Reprod.
0% 21.8
ontrol Control
0% 21.2
fitment 2 Treatment 2
rol CV
6.1
3rd Brood PASS FAIL
100% X
Test Start Date
24-Jul-24
Sample 2 25-Jul-24
1st 2nd
Tax Toy
Dilution Sample Sample
82.4
311 82 80
<.05 <.05
0.7 2.6
mmmmm�
MM
mm
pH
9 •
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
acili : CHARLOTTE WATER
NPDES# NC0084387
am le ID: Lee S. Dukes
l
ETT# T67932 Date: 24-Jul-24
aborato :
Certification #: NCO22
Ex . Date: I1/l/2024
Survival Data
7 Day Survival Test Used: FISHERS TEST
Control 100% Test Statistic: P= 1.000
Effluent 100% Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test for Normality
Mean young/female Std. Dev.
Test Used: Shapira-Wilks Test:
Control 21.8
1.34
Effluent 21.2
1.27
W: 0.952
Critical Value: 0.884
The data are normally distributed.
Analysisfor Differences in Reproduction
Test for Homogeneity of Variance
Test Used: Equal Variance t Test
Test Used: F Test
Calculated t =
1.25
F= 1.11
Critical Value--
2.51
Critical Value-- 4.40
The data are homogeneous in variance
PASS
Reproduction is not reduced in the effluent
Page 3 of 6

Control Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
source
rep
G 1 7-11
1
+3+9
11
23
V7 7-15
2
4
+7+12
23
119 7-17
3
+4+7
11
22
H7 7-11
4
+3+8
10
21
J4 7-15
5
+3+7
9
19
GG8 7-17
6
+5+8
11
24
K2 7-11
7
+2+10
1
10
22
V10 7-15
8
+4+6
11
21
GG6 7-17
9
+5+6
11
22
J9 7-11
10
+4+7
10
21
V8 7-15
11
+3+8
10
21
HH10 7-1
12
+3+9
11
23
13
N/A
14
N/A
15
N/A
16
N/A
17
N/A
18
N/A
19
N/A
Mean
20
N/A
1 21.8
37 % Effluent Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
G1 7-11
1
+4+7
10
21
V7 7-15
2
+4+7
10
21
119 7-17
3
+4+8
1 t
23
H7 7-11
4
+4+6
11
21
U4 7-15
5
+3+7
9
19
GG8 7-7
6
+3+9
11
23
K2 7-11
7
+4+7
1
10
21
V10 7-1
i 8
+3+8
10
21
GG6 7-7
9
+3+8
11
22
J9 7-11
10
+4+7
11
22
V8 7-15
11
+3+7
91
19
HMO 10 7.17
12
+3+8
10
21
0
13
N/A
0
14
N/A
0
15
N/A
0
16
N/A
0
17
N/A
0
18
N/A
0
19
N/A
Mean
0
20
N/A
21.2
renew Initial
JC
JC
End Date
fed by
C JC JC JC JC
07:45 AM 01:50 PM 06:00 AM 06:05 AM 09.30 AM 07.15 AM
31-Jul--24
11:38 AM JC
time fed & renew
New eff. `C 24.8
24.8
25.1
Old temp. •C
25
25.3
25.1
New Cont 24.5
24.6
24.5
Initial I I Dav 2 DavS I I Final
New pH 7.7
7.7
7.7
DO 8.1
8.6
8.5
0
Old PH
8.3
8.2
8.3
DO
8.8
8.9
8.9
0
C�
New pH 7.5
7.8
8.0
DO 8.1
8.7
9.2
a�
Old PH
8.2
8.3
8.3
DO
8.6
9.1
8.8
W
Lab#
T67932
Client
CHARLOTTE WAT
Sample ID
Lee S. Dukes
NPDES#
NCO084387
CountyMecklenbur
Month
7
Start & fed Date
24-Jul-24
Start & fed Time
Started & fed By
01:15 PM
JZ
Test Organism
Cerioda hnia dubia
Neo, born date
23-Jul-24
Neo. born time
1710-2200
TestType
NCCPF
Dilution Water
MHSF
Units for Conc.
%
IWC
37
%3rd BROOD
Test vessels
30 ml
Test volume
15 ml
Incubator#
1
Light
Initial Temp °C
161t/8dk
24.8
Selenastrum
AT
JEPA
0.05 ml
0.05 ml
Test method
B21-11-02-010:1002
& 67933 BLOCKED
or not used
Page 4 of 6

ETT
��'r � Plfl*1317Yfl�Ttl�
PO box 18414, GreenWlle, SC 2980e-7414
[phone], [phone]
Shipping Address: 4 Craftsman C4 Greer, SC 29850
eeestllah.ruy
CHAIN OF CUSTODY RECORD
Page_ of _Z
7-1
Client:
/C e r
Program
Containers
Pres--allve
Parameters
Facility:
r
whole Emuent Toxicity
C
n
State: NPDFSN:
Arvre
cnranm
re,torgani:�
U(Composite
only)
(Grub or Camposim)
G
o
.C.
U
U
E
t
U
G
a
Sign, and Print below
=
3 g
co
o
i. ttssa
-lta
a _
ri
i
c, m
g
° c
to
>
<
the dotted line
E
U •e—'
N E
„w
o u-
: c=
o
C U o
2
SAMPLE ID
e.,op",eswo.e rime
s.myee.ueni..n.i. Time
courted by
a�-
u H
,E
Z°
R a
U >
OH
— 6_0
< <
U
u
U 0
f
_ u
N
= 3
i ChemlealAnalyslsaONer
0------------
X
41L
X PJA
IX
X
-------------
-------------
LLL
1--.
$pecioll Wctions: /
/
Z
d .�
-k,!Xd
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Relinquished B / Organization By / Oreanization �' �C7 (
Area
Temp °C
Preserved?
/Received
�—
Q
7c� 2
f
Q Cd
p�
y Y�2u i C•L.T W
717,3121
noc�
_ C LrW
OMPOSITESAMPLIN ROCEDURES
T£AIP£RATU MONITORING PROCEDURES
HOLD T/MEPROCEDURES
Composite samples m e collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours. Equal volin
0.0 and 6.0 *C. Samples must not be frozen. Use water ice in sealed bogs.
of sample collection (completion of composite sample).
oral minimum I sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in NPDE$ permit.
Page 5 of 6

ETT
PO Box 16414, Gteemme, SC 29a0&7414
[phone], [phone]
SWMIng Morass: 4 Craftsman Ct, Greer, SC 291350
waw.ettlsb.arg
CHAIN OF CUSTODY RECORD
Page of 77171
Client: �L,r,�1 _�.�{a��..
CC1'e"J61 G Wmi5x
Program
I Conta
Prc,s—tive
Parameters
Facility: 1. 1 c DuICcS
Whole Emornt Toxicity
0
O
tl
State: 14PDFS q:
Acme
Chro.lcl
Test Orianisma
Li
8
c
0
U(Composite
only)
(Gob aComposi¢)
G
o
rC+
U
U
c
O
Sign, and Print below
3 =
c ^e
c
a• Irct.
)-"03
€
F
u
U
e0
�n
$ c
w
r
e
.e
o
the dotted line
E r✓
co
U `-'
0 2
"= a_
-
-
`e_
`e
'- _
5 c
` = e
SAMPLE ID
o
c en,p«hewn o.� Tfma
S.nple Celknl.0 o.ie Tme
CollaeleJ by
U in
A Z
ac a
(7 >
06.
<
<
U
U
U 6
LE E
_
in _. U
3
f Che.lcatA slyals&Other
iiu
�C'l," _
X
6%�
X dJA
X
6'1931$
2.
t
Special instructions:
Sample Custody Transfer Record 8 ! /�a ,ems
Secure
Receipt
Sample
Date Time Relinquished B / Organization ived B / ®tion GQ ��
Area
Tem °C
Presened7
1S 2
�
�45 rtril-o"f'r
��
r
r D
�A,Iti t,�� �LLTW
r17-
�b
COMPOS/TESAMPL/H ROCEDURES
TEMPERATU MON/TOxmGPROCEDURES
HOLDT/MEPROCEDURES
Composite samples mu k collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing die sample must first be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volu
0.0 and 6.0 *C. Samples must not be frozen. Use water ice in sealed bags.
of sample collection (completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in TJPDES permit.
Page 6 of 6

Attachment E
Section 11: Additional Information

Sasser, Gabe
From: Bell, Wes <[e-mail]>
Sent: Wednesday, August 7, 2024 11:48 AM
To: Sasser, Gabe
Subject: [EXT]RE: [EXT]RE: [EXT]RE: [EXT]Re: [External] Renewal Application
EXTERNAL EMAIL: This email originated from the Internet. Do not click any images, links or open any attachments unless you recognize and trust the sender and
know the content is safe. Please click the Phish Alert button to forward the email to Bad.Mail.
Gabe,
Go ahead and include what you have so far this year as well. You can have 3.5 years of data review, no problem.
Wes
Wes Bell
Env. Specialist II
Mooresville Regional Office
Water Quality Regional Operations Section
NCDEQ-Division of Water Resources
[phone] Office
[phone] Office Direct Line
Email: wes.bell deq.nc.gov
610 East Center Avenue
Suite 301
Mooresville, NC 28115
NORTH CAROIrINA
Dgmdmenl of EmriraaosntaI OuaItty
Email correspondence to and from this address is subject to the North
Carolina Public Records Law and may be disclosed to third parties.
From: Sasser, Gabe <[e-mail]>
Sent: Wednesday, August 7, 2024 11:30 AM
To: Bell, Wes <wes.bell @deq.nc.gov>; Deuterman, Sydney <[e-mail]>
Cc: Corporon, Joe <[e-mail]>
Subject: RE: [EXT]RE: [EXT]RE: [EXT]Re: [External] Renewal Application

CAUTION: External email. Do not click links or open attachments unless verified. Report suspicious emails with the Report Message
button located on your Outlook menu bar on the Home tab.
Hi Wes,
Hope you are doing well. Thanks for providing clarification and confirming the requirements for me. Very much
appreciate your help there. We can certainly provide the 3 years of data for the parameter and flow questions in the
application. Are you good with the last three complete calendar years (2021, 2020 and 2023)? Thanks and take care.
Gabe Sasser / Operations Manager
CHARLOTTE WATER
5200 Brookshire Blvd. / Charlotte, NC 28216
P:704-399-2142 / C:704-507-5267 / charlottewater.org
From: Bell, Wes <[e-mail]>
Sent: Wednesday, August 7, 2024 7:36 AM
To: Sasser, Gabe <[e-mail]>; Deuterman, Sydney <[e-mail]>
Cc: Corporon, Joe <[e-mail]>
Subject: [EXT]RE: [EXT]RE: [EXT]Re: [External] Renewal Application
EXTERNAL EMAIL: This email originated from the Internet. Do not click any images, links or open any attachments unless you recognize and trust the sender and
know the content is safe. Please click the Phish Alert button to forward the email to Bad.Mail.
Good Morning,
It has been confirmed that you only report what you were required to sample in your permit and you can put "NA"
for those parameters listed but not required to sample for. You will still need to check whether or not you believe
the listed parameters are in the waste stream (Presence or absence) for the parameters in Tables B and C. You
need a minimum of three years of data to use in the application.
Wes
Wes Bell
Env. Specialist II
Mooresville Regional Office
Water Quality Regional Operations Section
NCDEQ-Division of Water Resources
[phone] Office
[phone] Office Direct Line
Email: [e-mail]
610 East Center Avenue
Suite 301
Mooresville, NC 28115

Pvc'?FiTH t.AFtC3LIN1�
Department of Environmental Duality
Email correspondence to and from this address is subject to the North
Carolina Public Records Law and may be disclosed to third parties..
From: Sasser, Gabe <[e-mail]>
Sent: Tuesday, August 6, 2024 3:24 PM
To: Bell, Wes <[e-mail]>; Deuterman, Sydney <[e-mail]>
Cc: Corporon, Joe <[e-mail]>
Subject: RE: [EXT]RE: [EXT]Re: [External] Renewal Application
You don't often get email from [e-mail]. Learn why this is important
CAUTION: External email. Do not click links or open attachments unless verified. Report suspicious emails with the Report Message
button located on your Outlook menu bar on the Home tab.
Hi Wes,
Thanks for the update. Regarding the timeframe for data, is the previous year sufficient given the consistency in our
results? Thanks.
Gabe Sasser / Operations Manager
CHARLOTTE WATER
5200 Brookshire Blvd. / Charlotte, NC 28216
P:704-399-2142 / C:704-507-5267 / charlottewater.org
From: Bell, Wes <[e-mail]>
Sent: Tuesday, August 6, 2024 3:13 PM
To: Sasser, Gabe <[e-mail]>; Deuterman, Sydney <[e-mail]>
Cc: Corporon, Joe <[e-mail]>
Subject: [EXT]RE: [EXT]Re: [External] Renewal Application
EXTERNAL EMAIL: This email originated from the Internet. Do not click any images, links or open any attachments unless you recognize and trust the sender and
know the content is safe. Please click the Phish Alert button to forward the email to Bad.Mail.
Good afternoon Gabe,
I'm in the process of confirming but you do not have to sample for the parameters Listed in the renewal application
if your permit does not require you to .... for your type of permit anyway. I Let you know once that is confirmed. You
would mark "NA" for each applicable parameter.
Wes

Wes Bell
Env. Specialist II
Mooresville Regional Office
Water Quality Regional Operations Section
NCDEQ-Division of Water Resources
[phone] Office
[phone] Office Direct Line
Email: [e-mail]
610 East Center Avenue
Suite 301
Mooresville, NC 28115
4: � E
NOR rH CAROLINA
oepaMMM of EnvlmnMental Duality
R E C E I V 2-p
SEi Q 4 ''24
NCDEC,'��� PDES
Email correspondence to and from this address is subject to the North
Carolina Public Records Law and may be disclosed to third parties.
From: Sasser, Gabe <[e-mail]>
Sent: Tuesday, August 6, 2024 2:55 PM
To: Deuterman, Sydney <[e-mail]>
Cc: Bell, Wes <[e-mail]>; Corporon, Joe <][e-mail]>
Subject: RE: [EXT]Re: [External] Renewal Application
Some people who received this message don't often get email from [e-mail]. Learn why this is important
CAUTION: External email. Do not click links or open attachments unless verified. Report suspicious emails with the Report Message
button located on your Outlook menu bar on the Home tab.
Hi Sydney,
I hope you are doing well. In the past I have worked with Derek on permit renewals and he has directed me to you for
the latest updates. With the new permit renewal form being much more complex the old form can you help me with my
questions below regarding completion of the permit for Dukes WTP (Permit # NC0084387). Thanks and take care.
Gabe Sasser / Operations Manager
CHARLOTTE WATER
5200 Brookshire Blvd. / Charlotte, NC 28216
P:704-399-2142 / C:704-507-5267 / charlottewater.org

From: Denard, Derek <[e-mail]>
Sent: Monday, August 5, 2024 2:11 PM
To: Sasser, Gabe <[e-mail]>
Cc: Bell, Wes <[e-mail]>; Deuterman, Sydney <[e-mail]>; Corporon, Joe
<[e-mail]>
Subject: [EXT]Re: [External] Renewal Application
EXTERNAL EMAIL: This email originated from the Internet. Do not click any images, links or open any attachments unless you recognize and trust the sender and
know the content is safe. Please click the Phish Alert button to forward the email to Bad.Mail.
Gabe,
It has been a few years since I worked with WTPs. Please contact Sydney Deuterman. She would be best
to help with these questions about WTPs. Also Joe Corporon is currently the point of contact for
Mooresville Regional office permitting questions for Compliance and Expeditated Permitting. I am coping
them on this message.
Sincerely,
Derek C. Denard
Environmental Program Consultant, Division of Water Resources
Water Quality NPDES Permitting Section
North Carolina Department of Environmental Quality
Office: [phone]
[e-mail]
--7
e.,.D E ` -
IQ A-)
F.Y„ LHIH C.AW,�LJN!},
DeparbrAwd all En ironmenLaI amidy
Email correspondence to and from this address is subject to the North
Carolina Public Records Law and may be disclosed to third parties.

From: Sasser, Gabe <[e-mail]>
Sent: Monday, August 5, 2024 1:30 PM
To: Denard, Derek <[e-mail]>
Cc: Bell, Wes <[e-mail]>
Subject: [External] Renewal Application
You don't often get email from [e-mail]. Learn why this is important
CAUTION: External email. Do not click links or open attachments unless verified. Report suspicious emails with the Report Message
button located on your Outlook menu bar on the Home tab.
Hi Derek,
I hope you are doing well. In working together in the past regarding renewal of Charlotte Water's NPDES permits at
both Franklin and Dukes WTPs, I am in the process of preparing both permit applications in the coming weeks. I am
currently focused on Dukes (NC0084387) as the expiration of the current permit occurs on February 28th, 2025. As
points of reference, I have attached the previous permit renewal application for Dukes WTP (Short Form C - dated
9/25/19) along with the existing NPDES permit. Understanding the 180-day submittal requirement ahead of the
expiration date, I plan to submit the application for Dukes sometime near the end of August. With the new format
(Application Form 2C) being quite a bit more involved than the short form 2C we are accustomed to, I wanted to reach
out to you with several questions:
1. All the data I have is centered around the current permit requirements. Several different groups of
contaminants are included on the new permit application (Groups A, B, C and D). For many of the constituents
within those groupings, we have limited or no data. Group by group, I have the following comments:
• Group A: Per the existing Dukes WTP NPDES permit, we are required to monitor for the majority of
these parameters, but not for BOD, COD and TOC, so I have not data for these constituents. Are results
for these parameters required before submitting the application?
Group B: Per the existing Dukes WTP NPDES permit, we are not required to monitor for any of the
parameters included on this list and therefore don't have any monitoring data for these parameters.
Group C: Per the existing Dukes WTP NPDES permit, we only monitor for Total Chlorine Residual,
Fluoride, Nitrate/Nitrite, Total Nitrogen, Phosphorus and Aluminum. We are not required to monitor for
any other parameters.
• Group D: Per the existing Dukes WTP NPDES permit, we are not required to monitor for any of the
parameters included on this list and therefore don't have any monitoring data for these parameters.
2. Is providing a years -worth of analytical data for the required parameters representative for the parameters we
currently monitor for? Our results are very consistent.
I feel good about our process flow schematic, (line drawing) biosolids management plan and facility map. This
application is quite different from what we are accustomed to and I'm just reaching out for some guidance. Thanks and
take care.
Gabe Sasser / Operations Manager
CHARLOTTE WATER
5200 Brookshire Blvd. / Charlotte, NC 28216
P:704-399-2142 / C:704-507-5267 / charlottewater.org

Email correspondence to and from this address may be subject to the North Carolina Public Records Law and may be disclosed to third parties by an authorized
state official.