NC0047597_Toxicity Lab Data_20240806

ace,
August 06, 2024
Charles Cocker
South Durham WRF
6605 Farrington Road
Chapel Hill, NC 27517
RE: Project: Quarterly
Pace Project No.: 92742126
Dear Charles Cocker:
Pace Analytical Services, LLC
6701 Conference Drive
Raleigh, NC 27607
(919 )834-4984
Enclosed are the analytical results for sample(s) received by the laboratory on July 16, 2024. The results relate only to the
samples included in this report.
Analyses were performed at the Pace Analytical Services location indicated on the sample analyte page for aria{ysis unless
otherwise footnoted.
Some analyses were subcontracted outside of the Pace Network. The test report from the external subcontractor is
attached to this report in its entirety.
The test results provided in this final report were generated by each of the following laboratories within the Pace Network:
If you have any questions concerning this report, please feel free to contact me.
Sincerely,
Jonathan W Biddix
[e-mail]
704-977-0978
Project Manager
Enclosures
cc: Jennifer Hunter, City of Durham -Compliance Services Lab
Accounts Payable, South Durham WRF
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the writlen consent of Pace Analytical Services, LLC. Page 1 of 38

ETenm,,nerttai , Inc.
POIFOI Box 16414. Greenville- SC 2-9606
[phone] . FAX (B84) B7745938
4 Craftsman Caun. Greer 5C Z9550
Ceriodaphnia dubia Chronic Pass/Fail Toxicity Test
EPA-821-R-02-013 Method 1002
Client: South Durham
Facility: WWTP
NPDES #: N00047597
Test Date: 17-Jul-24
Laboratory ID #: T67884
Test Reviewed and Approved By:
Robert
Robert W. Kelley, Ph.D.
Laboratory Manager
.e
crrrsr��ugrt aF:xraly
Tesi rr;ull; pre -wd in 1hi, report cnnF rm iu all riquirzm rnu of
NF.L.�C.. nnJnclad under NF.LAC C.,66--Number E97810
FInr�Ju ilcpl. ntlirnllh IncluJed resuhs pert:nn unl.in pnnidnJ sampl..
Amy McMahan
QA/QC Review
SCD-HEC CertiCcation 413104
NCDENR cc,tinc.aonm o22
Page 1 of 6
Page 33 of 38

Effluent Toxicity Report Form - Chronic PasslFail and Acute LC50
Dale 31-Jul-24
Facility: South Durham WWTP NPIDES ff N00047597 Pipe # 001 Counly, Durham
Laboratory Performing Test:
X
Signature of Operator in Responsible Charge
X 4�rhert �e��e
Sianature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch Di, or
Water Duality N.C,
DENR
1621 Mall Service Center Raleigh, North
Carolina 27699-1621
North Carolina CoriQdaphnia Chronic PasslFail
Reproduction
Toxicity
Test
GoNTRI)L ORGAN ISMS
1
2
1
4
5
6
r
5
9
19
T2
s ro""9 I'll" d
24
23
22
23
22
22
24
22
22
24
23
24
A-1i IL)w toleae
L
L
L
L
L
L
L
L
L
L
L
L
Effluent h
zp�ATM6NT 2 CROAN i5NI 3
1
2
3
4
5
6
7
a
9
Ie
12
El-.�ea
21
22
21
23
22
19
22
22
19
22
23
22Med
L
L
L
L
L
L
L
L
L
L
L
L
start end start end start end
pH let sam le lstsam le 2nd sample
Control
Treatment 2
start end start end sta rl end
D•0. 1st sam le 1st sam le 2nd sample
Control
Treatment 2
LC50IAcute Toxicity Test
DEM Form AT-1
smplels This tag E i[hei Yes;
ollection (Slartl Date
ample 1 15-Jul-24
.mole T'.e mug«u.m.
Sample 1
Sample 2
Mao
Comp
Duraiion
X
24hrs.
X
24hrs.
Hardness (mg)Ll
Spec, Cond, (pmhos)
Chlorine (mg&I
Tamp. at receipt ('C)
)nic Test Results
�ulated t = 3.
cal Value= 2.5
;eduction= 6.1
lortality Avg. Reprod.
0% 22.9
.ontrof Control
0% 21.5
dment2 Treatment
rol CV
3.9
3rd Brood PASS FAIL
10Q%
Test Start Date
17-Jul-24
Sample 2 17-Jul-24
1st 2nd
Tux Tox
DlMI- Sample Sample
315 5301 584
<.05 <.05
1.0 2.0
m
pH
D.0.
Page 2 of 6
Page 34 of 38

STATISTICAL ANALYSIS RESULTS
Facility: South Durham
NPDES9 NCO047597
Sample ID: WWTP
ETT# T67884 Date: 17-Jul-24
Laboratory:
Certification #: NCO22
Exp. Date: 1111/2024
Survival Data
7 Day Survival
Test Used: FISHERS TEST
Control 100%
Test Statistic: P— 1.000
Effluent 100%
Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test for Normality
Mean young/female Std. Dev.
Test Used: Shapiro-W ilks Test:
Control 22.9
0.90
Effluent 21.5
1.31
W : 0.907
Critical Value: 0.884
The data are normally distributed,
nalysis for Differences in Reproduction
Test for Homogeneity of Variance
Test Used: Equal Variance t Test
Test Used: F Test
Calculated t =
3.08
F= 2.13
Critica[ Value—
2.51
Critical Value= 4.40
The data are homogeneous in variauce
PASS
Reproduction is not reduced in the effluent
Page 35 of 38
Page 3 of 6

Control Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
source
rep
B64 7-5
1
+4+8
12
24
C9 718
2
+5+7
71
23
GG2 7-10
3
+4+7
11
22
J1 7-4
4
+3+8
12
23
GG6 7-10
5
+4+7
11
22
11 7-4
6
+5+6
11
22
AA6 7-5
7
1
+3+9
1
12
1
24
H3 7-4
8
+4+7
11
22
W 1 7-8
9
+3+7
12
22
F4 7-8
10
+5+8
11
24
L2 7-4
11
+3+9
11
23
V9 7-8
12
+5+7
12
24
13
NIA
14
NIA
15
NIA
16
N!A
17
NIA
18
NIA
19
NrA
Mean
20
NIA
zz.9
90 1 Effluent Mortality and Reproduction by Tost Day
1 2 3 4 5 6 7 8
Totai
BB4 7-5
1
4
+7+10
21
C9 718
2
+3+6
11
22
GG2 7-
3
+4+7
10
21
A 7-4
4
+3+8
12
23
GG6 7-
5
+5+6
11
22
11 7-4
6
4
+6+9
19
AA6 7-5
7
+4+8
10
22
H3 7-4
e
+3+8
11
22
W1 7-8
9
+3+6
10
19
F4 7-8
16
+4+7
11
22
L2 7-4
11
+4+7
12
23
V9 7-8
12
+5+7
101
22
0
13
NIA
0
14
N!A
0
15
N1A
0
16
NIA
0
17
NIA
0
16
N1A
0
19
NIA
Mean
0
20
NIA
21.5
renew mitiai
JC
JC
End fate
fed by
JC
JC
JC
KG
JC
JC
24-Jul-24
1014 AM iZ
time fed & renew
07:30 AM 01:33 PM 06:00 AM 01.00 PM 10:27 AM 07:40 AM
New eft. ^c 24-8
25.0
24.9
old temp, C
25.1
25
25.2
New Cnni 24.6
24.5
24.4
Comments
Control ini temp
N=Dead NIA -Lost or not used
Page 4 of 6
Page 36 of 38

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