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NC0007064_Compliance Report_20240723
DUKE Brunswick Steam Electric Plant 8470 River Road SE ENERGY® Southport,NC 28461-8869 'JUL 2 3 2024 Serial: RA-24-0051 Christopher Johnson,Chief Water Science Section North Carolina Division of Environmental Quality Division of Water Resources 1621 Mail Service Center Raleigh, North Carolina 27699-1621 Subject: Brunswick Steam Electric Plant (BESP), Unit Nos. 1 and 2 2022-2023 Biological Monitoring Report Dear Mr.Johnson: Enclosed are three copies of the Brunswick Steam Electric Plant's 2022-2023 Biological Monitoring Report.The report summarizes the results of the monitoring program required by National Pollutant Discharge Elimination System Permit(NPDES) No. NC0007064. Biological monitoring during 2022-2023 continued to show a reduction in the number of entrained and impinged fish and shellfish resulting from the combination of flow minimization,fine-mesh screens, and the fish diversion structure.The fish return system continued to ensure that large numbers of the most valuable commercial species exhibit the greatest survival and were returned alive to the estuary.The report results indicate that operation of the BSEP continues to have no detectable adverse effect on the populations of fish, shrimp, and crabs residing in estuarine nursery areas. Please contact Mr. Kyle Hussey at(910) 832-3305, or Mr. Eric Caison (910) 832-2267, if there are any questions concerning the data contained in this submittal. Sincerely, A� Jay Ratliff Plant Manager Brunswick Steam Electric Plant cc: Kathy Rawls, North Carolina Department of Marine Fisheries, Director Enclosure: Brunswick Steam Electric Plant 2019 Biological Monitoring Report(three copies) DUKE Brunswick Steam Electric Plant 8470 River Road SE ENERGY® Southport,NC 28461-8869 ENCLOSURE Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report BRUNSWICK STEAM ELECTRIC PLANT 2022-2023 BIOLOGICAL MONITORING REPORT 41: r � NPDES Permit # NC0007064 August 2024 Prepared by: DUKE ENERGY PROGRESS, LLC. Environmental Field Support - Water Resources New Hill,North Carolina Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report rrt� Preface This copy ofthe report is not a.controlled document as detailed in the Environmental.Science Field Program Quality Assurance Manual. Any changes made to the original of this`report after the date of issuance can be obtained from: DUKE ENERGY Water Resources 3932 New Hill-Holleman.Rd New Hill,North Carolina 27562 Duke Energy Progress,LLC i Water Resources Brunswick Steam Electric Plant. 2022-2023 Biological Monitoring Report Table of Contents Page Preface....................................:...........:...::..............................:............................................. i Tableof Contents................ ............................................................. .... Listof Tables.............. ---......... .... . . ........ .............................................. Listof Figures:..................................................................................:...... iv Listof Appendices..:........:...:............................................................................................ . v ExecutiveSummary......................................................................................... 1.0 INTRODUCTION................................................................................................ 1 2.0 MONITORING PROGRAM............................................................................... 1 2..1 introduction.............................................................................:...............:............ 1 2:2 Methods.............................................................................................:...:.....:........ 1 2.3 Results and D:iscussion....................................................................6.................6. 5 2.3.1 Water Quality....................................................................................................... 5 2.3.2 Dominant Species...........:....:.... 2.3.3 Seasonality and Abundance.....:........................................:.......................... 6 2.3.4 Survival Estimates..........:........................:...........:.:..::......................................... 7 2.3.5 Annual Entrainment and Impingement Rate Comparisons :..............................:... 7 2.4 Summary and Conclusions ...............:..................................::....................:.............. 9 3.0 REFERENCES ......................................................... ............................ .. 31 4.0 APPENDICES..................................................................................................... A-1 Duke Energy Progress,LLC ii Water Resources Brunswick Steam Electric Plant 2022-2023 Biolohical Monitoring Report List of Tables Table Page 2.1 Representative species and life stages entrained and impinged at the Brunswick Steam.Electric Plant showing spawning location and season........... 11 2.2 Annual mean density and percent of total for the ten most abundant taxa/life stages collected during entrainrrient.sampling at the.Brunswick Steam Electric Plant. 2022-2023........................ ...:....:::..:............................................................ 12 2.3 Total number and percent of the ten most abundant taxa/life stages collected during larval impingement sampling at the Brunswick Steam Electric Plan, 2022-2023, ... ... ...... ......... ........................................................................ 13 2.4 Total number,total weight,and percent:of total of the ten most abundant juvenile.and adult organisms collected in the Brunswick Steam Electric Plant impingement samples,2022-2023.....:............. 14 2.5 Mean.densities of selected larval taxa entrained and impinged.at.the Brunswick.Steam Electric Plant,2022................................................................. 15 2.6 Mean densities of selected larval taxa entrained and impinged at the Brunswick Steam Electric Plant,2023............:. 2.7 Density(no./million.m3)and modal length(mm)for representative important taxa collected by month with juvenile and adult impingement sampling at the Brunswick Steam Electric Plant, 20.22::...:...........:.........:.... 19 2.8 Density (no./million m3)and modal length(ruin)for representative important taxa collected by month with juvenile and adult impingement sampling at the Brunswick.Steam Electric Plant,2023............. 2.9 Mean percent survival and percent total number of larval organisms collected during larval impingement sampling at the Brunswick Steam Electric.Plant,2022-2023 .............::..:...................................... 21 .2:10 Mean percent survival and percent of total number of organisms collected during impingement sampling at the Brunswick Steam Electric Plant, 2022-2023.......... ................ .. . ............ . ................... . ................... 22 2.11 Mean annual percent reduction in the number of representative taxa entrained and reductions in impingement mortality at the Brunswick Steam Electric Plant, 1984.-2023 ..................................:.................... 23 Duke Energy Progress,LLC iii Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report List of Figures Figure Pave 1.1 Location of the Brunswick Steam Electric Plant near Southport, NorthCarolina............ ...... ..... . ..............................a........................ 3 2.1 Location of the fish diversion structure,fish return flume, return basin, and sampling locations at the.Brunswick Steam Electric-Plant.................................. 4 2.2 Mean daily freshwater flow(curs)to the Cape Fear Estuary,2022-2023 ........... 24 2:3 Annual mean daily freshwater flow to the Cape Fear Estuary,2001-2023......... 24 ............. ................. . .. .............................................................................. 2.4 Mean monthly intake canal water temperature and salinity.,2022:..::................. 25 2.5 Mean monthly intake canal water temperature and salinity,2023 ....4................. 25 2.6 Annual number of total organisms entrained.at the Brunswick Steam Electric Plant and.percent reduction from baseline entrainment estimates, 1979-2023.................................................... ............... ..... . ....... ...... 26 2.7 Annual number of Spot entrained at the Brunswick Steam Electric Plant and percent reduction from baseline entrainment estimates, 1919-2023...:...............:.................................:....................................................... 26 2.8 Annual number of Atlantic Menhaden entrained at the Brunswick Steam. Electric Plant and percent.reduction from baseline entrainment estimates, 1979-2022.......................................................... .. . .......... . .. 27 2.9 Annual number of portunid crab megalops entrained.at the Brunswick Steam Electric Plant and percent redaction.from baseline entrainment estimates, 1.979-2022.............................................:....:..........:....:....:.......:.....:..:...:............... 27 2.10 Annual number of commercial shrimp postlarvae entrained at the Brunswick Steam Electric Plant and percent reduction from baseline entrainment estimates, 1979-2023............................:....;.................................................... 28 2.11 Annual number of Atlantic Menhaden impinged at the Brunswick Steam Electric Plant and percent reduction from baseline impingement estimates, 1977-2023..............................................................:.............:........ ................... 28 2.12 Annual number of Bay Anchovy impinged at the Brunswick Steam Electric Plant and percent reduction from baseline impingement estimates, 1977-2023........................................ .. ............. ............. ...... 29 Duke Energy Progress,LLC iv Water Resources Brunswick Steam Electric Plant 2022-2023 Biolouical Monitoring Report MOMMAMEM List of figures (continued) Figure Page 2.13 Annual number of Spot impinged at the Brunswick Steam Electric Plant and percent reduction from baseline impingement estimates, 1977-2023......................................................................•..:.............................:...: 29 2.14 Annual nuinber of commercial shrimp impinged at the Brunswick Steam Electric.Plant and percent reduction from baseline impingement estimates, 1977-2023.........................................................................:.................. 30 2.15 Annual number of blue crabs(all Callinectes spp. Combined)impinged at the Brunswick Steam Electric Plan and percent reduction from baseline impingement estimates, 1977-2023 ..........:.........:......:.....:.................................... 30 List of Appendices Appendix Paae 1 Summary of historical environmental studies conducted in association with the Brunswick Steam Electric Plant, 1.968-2023:..........:....:....... 2 Total number of larval.organisms collected during entrainment and larval impingement sampling at the Brunswick Steam Electric Plant,2022....:............ A4 3 Total number and biomass of juvenile and.adult organisms collected.during impingement sampling at the Brunswick Steam Electric Plant,.2022................. A-6 4 Total number of larval organisms collected during entrainment and larval impingement sampling.at the Brunswick Steam Electric Plant, 2023................. A-9 5 Total rnunber and biomass of juvenile and adult organisms collected during impingement sampling at the.Brunswick Steam Electric Plant,.2023.......:......... A-11 Duke Energy Progress,LLC v Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Executive Summary Entrainment and impingement studies from 2022 through 2023 continue to demonstrate the effectiveness of the Brunswick Steam Electric Plants system of technologies and operational measures at reducing the number of organisms impacted by the withdrawal of cooling water from the Cape Fear Estuary. Evidence supporting this conclusion include species composition and abundance of organisms entrained and impinged. The species .composition and abundance of organisms collected during entrainment, larval impingement, and Juvenile/Adult impingement studies in 2022 and 2023 were similar to previous years and correspond to the natural.seasonality of organisms in the. Cape Fear Estuary. Station intake and operational modifications including seasonal. flow .reductions,. fine-mesh traveling screens, fish. return system, and fish diversion structure continue to be effective in reducing entrainment and impingement mortality when compared to baseline conditions. Moreover, based on the evaluation of the 316b study reports submitted to the North Carolina Department of Environmental Quality,the Department concluded in 2023 that the Brunswick Steam Electric Plant represented best technologies available for meeting the Clean Water Act 316b rule. Duke Energy Progress,LaL,C vi Water Resources Brunswick Steams Electric Plant 2022-2023 BioluTical MonitorinE Report 1.0 INTRODUCTION Duke Energy Progress,Inc. (DEP)owns and operates the Brunswick Steam Electric Plant (Brunswick) located adjacent to the Cape Fear .Estuary (CFE) in Brunswick County, North Carolina (Figure 1.1). As required by Brunswick's National Pollution Discharge Elimination System(NPDES)Permit No.NCO007064 Part 1,,Section A. (1): DIVERSION FENCE-A.diversion.fence located at the mouth of the intake canal shall be continuously operated and.maintained an:such a.manner as to minimize impingement.A biological monitoringprogram shall be continued which will provide sufficient information to allow for.a continuing assessment of the. impact of the Brunswick Steam Electric Plant on the Cape Fear estuary, with particular emphasis on.the marine fisheries, Data shall be reported annually and shall include an inteypretive summary report assessing the effectiveness of the diversion fence, and the effectiveness offlow minimization and fine mesh screens to curtail organism impingement and entrainment. The Director shall approve any major changes in the,biological monitoring program. This report satisfies this permit requirement. The primary objective of the biological monitoring program is to provide an assessment of the continued effectiveness of the fish diversion structure;fine-mesh screens,and seasonally.based flow minimization in reducing the impingement, and entrainment of organisms. Secondary objectives of the program were to document environmental factors (e.g., freshwater flow. water temperature, and salinity patterns) impacting the density or seasonality of the.fish community that were not attributable to plant operations. A detailed summary of historical environmental studies can be found in Appendix 1. 2.0 MONITORING PROGRAM 2.1 Introduction Entrainment sampling.assessed the density,and seasonality of larval and postlarval.organisms passing through the cooling system. The larval impingement.Study evaluated the effectiveness of the fine-mesh screens in reducing entrainnent of these organisms. Organisms collected by larval impingement sampling were organisms that would have been entrained without the use of fine- mesh.screens. Juvenile and.adult (J/A) impingement sampling documented species. abundance, site, and weight of juvenile and adult organisms impinged on conventional 9A-mm (3/8-inch) mesh traveling screens. J/A impingement sampling also provided evidence of the continued effectiveness of the fish diversion stricture. Historical survival study results were presented to provide insight regarding the effectiveness of the fish return system .in reducing impingement mortality(CP&L 1987, 1988). 2.2 Methods Intake canal water temperature and salinity measurements were collected from the fish return flume during impingement sampling. Daily freshwater flow values presented in.the.report:were downloaded from the United.States Geological Survey website. Total freshwater input to the CFE was.estimated using data from stream gaging stations in the Cape Fear(2105769),Noitheast Cape Fear(210.8000), and B1ack.Rivers (2106.500) according to the methods presented by Giese et al. (1979, 1985.). Duke Energy Progress,LLC 1 `eater Resources Brunswick.Steain Electric Plant 2022m2023 Biolo ical Monitoring Report The collection gear used for entrainment and impingement sampling.has remained unchanged since 1984(CP&L 1985a).Entrainment sampling was:conducted in the discharge weir.Larval and J/A impingement sampling were conducted in. the fish return flume (Figure 2.1). The J/A impingement program evaluated fish and shrimp>_41 mm,portunid crabs.?25 mm, and eels and pipefish>_ 101 mm.Impinged organisms smaller than these size limits would have been entrained without use of fine-mesh screens and are evaluated by the larval impingement sampling program. These size class cut-off lengths were determined from examination of historic entrainment and impingement length-frequency information. Densities calculated for all larval organisms entrained.and impinged were averaged to obtain a mean number per 1,000 m3 of water pumped through the plant per sampling day. Densities for J/A organisms impinged on each sampling day were calculated by dividing the total,number of organisms collected by the volume of water pumped through the plant. Densities were expressed as the number per million cubic meters of water pumped through the plant.during:each 24-hour sampling period.. An overall assessment of reductions in. entrainment. and impingement from.the system of technologies.and operational measures in place was calculated using baseline data collected prior to plant.modifications.A detailed description of how those values were calculated can be found in Appendix 1. Ten taxonomic groups have been selected for the detailed comparisons of annual numbers entrained and impinged to.a calculated baseline as described above(Table 2.1).These taxonomic. groups were selected as representative taxa(RT)and life stages based upon 4 main criteria. First, they account for roughly 78%and 66%,respectively,of-the larvae and nekton collected in the CFE during the original Cape.Fear Studies conducted prior to 1980 (CP&L 1980). In addition,.these organisms represent approximately 80% of the larvae and juvenile/adult organisms collected by entrainment and impingement sampling (CP&L. 1.980, CP&L 19.$5b, .CP&L 1994, DEP 20.19). Secondly,these taxonomic groups encompass the spectrum of life history strategies evident within the CFE with respect to spawning location and.season. Estuarine dependent species representing. both temporary and resident species are included within these taxonomic :groups._ The third criterion for consideration is that most of these taxonomic groups,except for anchovies and gobies, are commercially or recreationally.important species. Finally, there.is precedent established for the use of these taxonomic groups as RT. Except for the addition of gobies (Gobiosoma spp.), blue crabs, and portunid crab megalops, these were the same taxonomic groups used by the principal investigators during the original Cape Fear Studies during the 197Ws(CP&L1980). This is important in that use of these taxonomic groups provides a direct link back to the original.studies conducted prior to: 1980: The additional two taxa (gobies and portunid crabs) were included beginning .in 1980 since relatively large numbers of these. taxa may be entrained seasonally. Common names are.used throughout the body of the report. The..associated scientific names may be found in appendices 2=3. Duke Energy Progress,LLC 2 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Brunswick Steam i—� Electric Plant L J NORTH CAROLINA o 41?/Pn C�BPR Q - Diversion O Fish Return Structure Plant Site'N'k Flume Intake Canal Discharge Weir 2k Q' 2na\ 2 �ac9eG 0� N(/ Elizabeth River Southport / o J✓ . o a Ocean Discharge Area N ° A Atlantic Ocean 0 1 2 3 4 Miles I i I I I I 0 1.5 3 4.5 6 Kilometers Figure 1.1 Location of the Brunswick Steam Electric Plant near Southport, North Carolina. Duke Energy Progress,LLC 3 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Entrainment Sanpiing Larval krpingen at Sanpiing inpingenwA Sanviing Dieehuge Wait Fish Rattan Flume Fish Rattan Fkure • ~ r � r !' i N A Walden Creek K sOtee �a�G� Gum Log Branch Fish Return Fish .-- Basin Diversion �e Structure 'J 1 Intake Canal r ��y O Plant Discharge Site Weir 4. 0 1,250 2,500 5,000 Feet �Qb 0 0 375 750 1,500 Meters VfD� Figure 2.1 Location of the fish diversion structure, fish return flume, return basin, and sampling locations at the Brunswick Steam Electric Plant. Duke Energy Progress,LLC 4 Water Resources Brunswick Steam Electric Plant 2022-2023 Biolo 'cal NEonitorinE Re ort 2.3 Results and Discussion 2.3.1 Water Quality e Freshwater flow to the estuary during 2022 and 2023 exhibited the typical seasonal patterns of higher flow during the winter/spring and lower flow during summer/fall (Figure 2.2). Daily annual mean freshwater flow for 2022 and 2023 was 130 cros and 1.92 cros respectively(Figure 2.3).Both years were below the 20-year daily annual mean of 247 cros. •Intake canal salinity ranged between 1.6 ppt and 36 ppt:during 2022 and 2023 (Figure 2.4-2.5). Salinity is typically higher in the summer months when freshwater flow to.the estuary is lower and decreases in the winter/spring.months when freshwater flow to the estuary is typically greatest. •Mean water temperature of the intake canal exhibited the typical seasonal pattern of peak cooler. teiperatures in January/February and peak higher temperatures during July/August (Figure 2.4-2.5). The lowest water temperature(10.0°C) was recorded in January 2022 and the highest water temperature (29.9°C)was recorded.in August 2023 during the 2022-2023 monitoring period. 2.3.2 Dominant Species •Gobiosoma spp., .Anchoa spp., commercial shrimp postlatvae (i.e., Brown, Pink, and White Shrimp), and Atlantic Croaker were the most abundant taxa .collected in entrainment samples for 2022 and 2023 .(Table 2.2). Spot,. silversides, and swimming crab megalops were also among the ten most numerous taxa entrained for both years. A complete.species Iist presenting the total number collected can be.found:in.Appendix 2 and 4. •Ten taxa accounted for approximately 95% of the total larval organisms collected in larval impingement samples during both years (Table 2.3). The most dominant larvae impinged on. the fine-mesh screens were commercial. shrimp (postlarvae), Atlantic Croaker, Anchoa spp.; and swimming crab megalops. Spot, and Gobisoma spp., were also among.the top ten larval taxa impinged. A substantial number of.larvae impinged on the:fine=rnesh.screens were returned alive.(CP&L 1988) to the CFE through the fish return flume(section 2.3.4)..A complete species list presenting the total.number collected can be.found in Appendix 2 and 4:. • J/A impingement samples collected during 2022 and 2023 were numerically dominated. by Bay Anchovy,.Lesser Blue Crab, White Shrimp, and Brown Shrimp (Table 2.4). Additional taxa that were among the top ten collected include.Spot, Pink Shrimp, and Brief Squid. Prior to :installation of the fish. diversion structure in 1082, Atlantic Menhaden was consistently the top species collected in terms of number and biomass. (CP&L 1980a, 1980b, 1982,. 1983). Larger, Age 1+ fin.fish, such as Spot and Atlantic Croaker also comprised a significant portion of the number and biomass collected by Duke Energy Progress,LLC 5 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report impingement sampling prior to 1983. This shift in species composition after.1983 was a result of the exclusion of larger finf sh by the fish diversion structure. This is significant because commercial shrimp and swimming crabs have become the more.numerically dominant taxa impinged other than Bay Anchovy. Both shrimp and crabs exhibit high survival rates (CP&L 1988) in the:fish return system. A.complete species list showing numbers and weights collected for all species can be found in Appendices 3 and 5. 2.3.3 Seasonality and Abundance *Seasonal variations for larvae entrained.and impinged during 2022 and 2023 followed.the expected seasonal patterns. Atlantic Menhaden, Spot,.Atlantic Croaker, and Pinfish, all ocean-spawned species., were most abundant during winter and early spring (Table 2.5- 2.6). Atlantic Croaker also exhibited a fall recruitment period consistent with,previous years. Estuarine-spawned species (e.g., anchovies, Gobiosoma spp., and. silversides) were most abundant during the.spring and summer.Postlarvae shrimp were present year around with a peak in spring. •Variation in the daily mean densities per month for larval organisms entrained and impinged during. 2022 and 2023 were comparable to :seasonal variations observed in previous years and corresponded to the seasonality of larval fish in the CFE, This observation is important since it indicates that recruitment to and within.the estuary has not been disrupted by cooling water withdrawal. Changes in peaks of abundance in eritrainment and impingement of organisms, are typical. and can be influenced by environmental conditions such as changing freshwater flow to the estuary.(Blumberg et al..2004; Copeland et al. 1979;Lawler et al. 198.8;Thompson 1989). *The seasonality of larger individuals collected during 2022 and 2023 J/A impingement sampling was consistent with data collected from previous years and the .natural seasonality reported for these species in the lower CFE by Schwartz et al.(1979). Results indicate that impinged finf sh were mostly young-of-year(YOY)or yearling individuals too small. to be excluded by the. fish diversion structure. Impingement of significant numbers of larger fish has been virtually eliminated by the installation of the fish diversion structure. Bay Anchovy,collected year-round,was.most abundant.during the. winter and spring when past data,indicated that most of.the.individuals in the population had grown to a size large enough to be impinged on the coarse-mesh sections of the traveling screens (Table 2.7-2.8). Peak densities of Bro..wn Shrimp occurred during the summer recruitment period while White Shrimp density peaked during.late summer early fall.Blue crabs were present most of the year but peaked in late spring through summer. •The seasonality for total number of all organisms impinged during 2022 peaked in May and was driven. by the large numbers of Lesser Blue.Crab and Bay Anchovy. During 2023,July was the peak month for total numbers,and was influenced by Brown Shrimp recruitment as.well as Lesser.Blue Crab numbers.Commercial shrimp or blue crabs were the dominant organisms impinged for seven months out.of the year for both years.This is significant because commercial shrimp and blue crabs are characterized by high survival rates in the fish return system (CP&L 1987, 1988). Duke Enerby Promress,1 A.0 6 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report 2.3.4 Survival Estimates *Seventeen of the impinged larval taxa were previously evaluated for survival from 1984- 1987 (CP&L 1988). Survival of Pinfish was estimated using data for Atlantic Croaker as a surrogate since Atlantic Croaker and Pinfish both_recruit to the estuary during the tivinter months. These eighteen taxa accounted for approximately 89% and 91%, respectively,of the total number collected with larval impingement sampling during 2022 and 2023 (Table 2.9). Survival during fast-screen rotation.ranged from approximately 1% for anchovies (Anchoa spp. > 13mm) to approximately 96% for Pink and White Shrimp. Survival adjusted for controls was 100% for commercial shrimp postlarvae, portunid crab megalops,and blue crabs. *Eleven taxa of the dominant J/A organisms impinged were previously evaluated for survival during fast-screen rotation (Table 2.10; CP&L 1987, 1988). Additionally, survival of Star.Drum and Silver Perch was estimated using data from two closely related surrogate species (Atlantic Croaker and Spot). Data for blue crabs was used as a surrogate for Portunus spp. crabs. These fifteen taxa accounted for approximately 9.3% and 91%;.respectively, of the total number collected with J/A impingement sampling during 2022 and 2023 (Table 2.10). 2.3.5 Annual Entrainment and Impingement Rate Comparisons *-The`rise of continuously rotating fine-mesh screens, fish return system, and reduced cooling water: flow has successfully reduced the number of organisms entrained since: 1983. Reductions in the mean annual number of RT entrained compared to a pre-1983 annual baseline entrainment ranged from.61%for Atlantic Croaker to 96%for portunid crab megalops (Table 2.11). RT exhibiting relatively high reductions (80-96%) in the annual number entrained compared to a calculated baseline include portunid crab megalops, flounder, mullet, commercial shrimp, Gobiosoma spp., :and anchovies. The annual number of entrained Atlantic Croaker, Atlantic Menhaden., seatrout, and Spot exhibited a moderate reduction(61-629/6).Mean annual reductions in the number of total organisms entrained was relatively high at 75%. • Variability in the annual number entrained was evident and consistent with the natural variability of larvae within the estuary (Figures 2.6-2.10). Natural variability in recruitment and natural mortality were determined to be significantly .greater than entrainment rates(i.e.,mortality)and there is no evidence of adverse effects on.the annual distribution and abundance of organisms in the CFE due to cooling water withdrawal even prior to.intake and operational modifications to reduce entrainment (CP&L 1980). Large-scale fluctuations in the number of larvae entering the estuary are natural and expected due to spawning success, offshore transport mechanisms, and climate conditions.(Nelson et al.. 1977,Norcross and Austin .1981,`Norcross and Shaw.1984). In .addition, changing freshwater flow patterns. and temperature during recruitment to the CFE'has a strong effect on the distribution of larvae within the estuary.(Copeland et:al. 1979,Lawler et al.1988,Rogers et al. 1984,Thompson 1989,Weinstein 1979,Weinstein et al. 1979, 1980).. Higher freshwater flow during the recruitment season of a species Duke Fuergy Progress,I,I,C 7 Water Resources Brunswick Steam Electric Plant 2022-2023 Biolozical Monitoring Report tends to limit dispersal of larvae to the upper estuary and increase the number and availability of larvae potentially entrained with the cooling water withdrawn from.the. lower estuary. The estimates of annual number entrained compared to baseline estimates have not been normalized for changes in larval recruitment, freshwater flow, or other environmental variables so variability is expected. The mean annual percent reduction values presented in Table 2.11 minimize the annual variability due to changing environmental conditions..Despite the annual variability evident for some taxa,there has been an overall.reduction.in the annual number of larvae entrained since 1983 for all RT. • Installation of the fish diversion structure, continuously rotating traveling.screens, and operation of the fish return system has substantially reduced.impingement mortality by reducing the numbers:of organisms 'impinged and.returning a large number of those organisms back to the CFE alive(CP&L .1988). Mean annual reductions in impingement mortality of RT since 1984 ranged from 37%o for Bay Anchovy to >_ 99% for Atlantic Menhaden, and blue crabs (Table 2.11). Mean annual reductions in impingement mortality for all other RT was>_90%over 40 years of monitoring. • Consistent large reductions (99%) in the annual number impinged. were evident for Atlantic Menhaden (Figure 2.11). A reduction in the annual number of Atlantic Menhaden impinged during 1979, 1980, and.1981 occurred as a result of temporary fish diversion structures installed for those years. A temporary, prototype fish diversion structure was installed and.operational only.during the period of abundance of Atlantic Menhaden.early in 1979. Severe .failure of the screens after the first few months of operation in .1979 limited its effectiveness for other species .(CP&L 1981). A. more substantial prototype structure was in place during 1980 and 1981. • Bay Anchovy has been the. numerically dominant species collected by impingement sampling since 1984. Small to moderate reductions in the annual number of Bay Anchovy impinged since 1984 is evident in some years,while annual impingement rates have been-highly variable(Figure 2.11). This was not unexpected since most individuals of.this species are small enough to pass through the 9.4-tiam mesh screens installed on the .fish diversion structure. .Bay Anchovy is also.characterized as a fragile species (USEPA.2014), exhibiting poor survival on the traveling screens so little reduction in impingement mortality was noted for this species. • Spot are a taxa that provide an example of some variability in the annual number: impinged per year (Figure 2.1.3).. When. an estimate of survival of the remaining impinged fish is factored in,overall reductions in impingement:mortal.ity are greater than 80%per year in most years. • Shrimp and blue crabs. exhibited high reductions (94-99%) in annual impingement mortality (Table 2..11). Shrimp are examples of organisms that are.not as effectively excluded.by the fish diversion structure (Figure 2.14). However, reduction in annual impingement mortality per year was> 90% due to the high survival rates (90-94%) for impinged shrimp. Duke Energy Progress,LLC 8 Water Resources Brunswick Steam Electric Plant 2022-2023 BiolovicalMonitorin Report 2.4 Summary and Conclusions Biological monitoring of the CFE has been conducted since the early 1970's. Results of intensive sampling throughout the 1970's and the early 1980's, prior to installation of intake modifications to reduce entrainment and impingement; indicated that power station operations had no measurable adverse effect on fish and shellfish populations in the CFE. Entrainment and impingement studies through 2023 continue to demonstrate the effectiveness of the BSEP intake modifications and operational measures in reducing the_number of organisms affected by the withdrawal of coolingwater from the CFE compared to baseline studies. Evidence. supporting this conclusion includes species composition and abundance of organisms entrained and impinged. The species composition and seasonality of organisms collected in the entrainment, larval impingement,and J/A impingement studies through 2023 were similar to previous years and corresponded with natural seasonality of larval organisms in.the CFE. Anchovies, gobles,.Spot, Atlantic Croaker,commercial shrimp and crabs were the dominant.larvae entrained and impinged. Use of fine-mesh screens.has.decreased the risk of entrainment for,all.larval taxa. This is especially important. for organisms :that spawn in the nearshore and offshore ocean such as. Atlantic Menhaden,. Spot, Atlantic Croaker, mullet,flounder, shrimp, and crabs. These organisms may potentially be at more risk of being.entrained since they are spawned offshore and are transported past the intake canal to the nursery areas in the CFE. Conversely,anchovies.and gobies are at less risk since they are estuarine residents and spawning occurs throughout the entire estuary. Use of fine-mesh screens and flow reduction.over three decades reduced the mean annual numbers of all organisms entrained by 60-96%. Consistently greater reductions in entrainment were evident for mullet, flounder, Gobiosoma spp., shrimp postlarvae and portunid crab megalops. Except for Gobiosoma spp., all are valuable commercial taxa. Larvae that would have been entrained.were returned to the CFE in significant numbers by the fish.return system. Based on historic:survival estimates data; substantial numbers of the larvae tested for survival were returned alive to the estuary. Numerically dominant and valuable.commercial taxa, flounder, shrimp, and swimming crab larvae exhibited the greatest survival. Considered together, the fish diversion structure and fish return system have substantially reduced the impingement mortality of larger organisms. The fish diversion structure excludes many of the larger fish and shellfish from the canal. Substantial numbers of fish and shellfish that do get into the.intake canal and subsequently become impinged are returned alive.to the estuary by the fish return system. In addition to .a reduction in numbers and mortality, a shift in the impingement of smaller.fnfish has resulted as well. This shift to smaller individuals is important because the larger individuals that are.being excluded from the intake canal by the fish diversion structure are the.reproducing members of the population. In addition, the natural mortality rates (predation; weather, tides, etc.)of smaller individuals are relatively high compared to the natural mortality rates of older, larger individuals,so many of the smaller individuals would not survive to maturity even in the absence of power station.operation.The species composition of organisms. collected by impingement sampling also shifted to a greater percentage of shrimp and blue crabs rather than larger fmfish as a result of the fish diversion structure. This shift in species composition is significant since juvenile and adult shrimp and crabs exhibit excellent survival in the fish return system (> 90%). Results indicate that operation of the BSEP continues to have no detectable adverse effect on the populations of fish,shrimp, and crabs residing in the CFE. Station intake and operational modifications including fine-mesh traveling screens, fish return system, fish Duke Energy Progress,LLC 9 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Re ort diversion structure, and seasonal .flow minimization continue to be effective in reducing entrainment and impingement mortality over many decades of operation. Interannual variability in species distribution and abundance is driven by environmental variables. with no detectable effect of facility cooling water withdrawal on the CFE. Duke E-uergy Progress,LLC 10 water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring eport Table 2.1 Representative species. and life stages'' entrained and impinged at the Brunswick Steam Electric Plant showing spawning location and season. Spawning Spawning Entrainment Impingement locationq season§ Anchovies(Anchoa spp.) Bay Anchovy E,NSO SP, SU Atlantic Menhaden Atlantic Menhaden OSO W Spot Spot OSO W Atlantic Croaker Atlantic:Croaker OSO W Seatrout(Cynoeion spp.) Seatrout E,NSO SP, SU,.F Mullet(Mugil spp.) Mullet OSO W Flounder(Paralichthys spp.) Flounder OSO W Gobiosoma spp E SP, SU Shrimp(Litopenaeusspp, Shrimp postlarvae Farfantepenaeus.spp.) OSO S.P, SU Portunid crab megalops Blue crabs(Callinecies spp) NSO F +Larval and juvenile life stages are smaller size.classes of organisms typically entrained with the cooling water whereas sub adult and adult size.classes of organisms are larger organisms impinged on traditional 9.4 mm mesh traveling screens. ISpawning location designations are estuarine(E),nearshore ocean(NSO),and offshore ocean (oso),. §Spawning season designations are winter(W), spring(SP),summer(SU), and fall (F). Duke Energy Progress,LLC ]1 Water Resources Brimswick Steam Electric Plant 2622-2023 Biological Monitoring Report Table 2.2 Annual mean density (no./1000 im3) and percent of.total'- for the ten most abundant taxa/life stages collected. during entrainment sampling at the Brunswick Steam.Electric Plant,2022-2023. 2022 Taxon Mean density Percent Gobisoma spp: 18.0 24.7 Anchoa`spp.(>_13mm) 13.5 18.5. Commercial sWrnpT postlarvae 10.2 13.9 Anchoa spp. (<1.3mm) 9.3 12.8 Spot 4.2 53 Atlantic Croaker 3.9 5.3 Swimming crabs(megalops) 2.6 3.5 Silversides 2.1 2.8 Ctenogobius/Gobionellus spp. 1.8 2.5 Silver Perch 1.6 2.2 Other taxa(n=30) 5.9 118. Total organisms" 73.1 100.0 2023 Taxon. Mean density Percent Commercial shrimp?postlarvae 16.7 18.3 Atlantic Croaker 16.4 17.9 Gobisoma spp. 15.8 17.3 Anchoa spp. (>_13mm) 14.3 15.7 Silversides 5.1 5,6 Spot 3.4 3.7 Swimming crabs(megalops) 3.4 3.7 Ctenogobius/Gobionellus,spp. 2.4 2.7 Anchoa.spp. (<l3mm) 2.3 2.6 Atlantic Menhaden 2.1 2.3. Other taxa(n=28) 9.3 10.2 Total organisms" 91.2 100.0 +Total may vary from summation due to rounding of individual taxon. rBrown,Pink, and White Shrimp. Duke Energy Progress,LLC 12 Nailer Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Re ort Table 2.3 Total number+and percents of the ten most abundant taxa/life stages collected during larval impingement sampling at the Brunswick.Steam Electric Plant,2022-2023. 2022 Taxon Total number} Percent Commercial shrimpX postlarvae 3,5.07,049 58.8 Swimming crabs(megalops) 717,428 12.0 Anchoa spp. (<13mm) 313,433 5.3 Anchoa spp. (>:Dmm) 257,870 4.3 Atlantic Croaker 2351356 3.9 Spot 204,764 3.4 Gobisoma spp. 186,042. 3.1 Swimming crabs 1.00,728 1.7 Gobinellus/Ctenogobioits spp. 86,908 1.5 Planehead 1~ilefish 65,542 1.1 Other taxa(n=24) 285,904 4.8 Total% 5,961.,025 100.0 2023 Taxon Total number' Percent Commercial shrimpX postlarvae 3,876,513 3.8.6 Atlantic Croaker 2,780,405 27.7 Swimming crabs(megalops) 1.,066,263 1.0.6 Anchoa.spp. (>_13mm) 476,679 4.7 Gobisomn spp: .332,137 3.3 GobinelluslCtenogobious spp. 284,861. 2.8 Spot 252,455 2.5 Atlantic Menhaden 187,908 1.9 Hardback Shrimp 174,831 1.7 Anchoa spp. (<13mm) 121,955 1.2 Other taxa(n=32) 490683 4.9 Totals 10,044,660 100.0 Dotal number is a sum of the twelve sampling-clay totals. 'ITotal may vary from summation due to rounding of individual taxon. ?Brown,Pink, and White Shrimp. Duke Energy Progress,LLC 13 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitorhl Report Table 2.4 Total number, total weight, and percent of total of the ten most abundant. juvenile and adult.organisms collected in the Brunswick Steam Electric Plant impingement samples,2022-2023. 2022 Taxon Number+ Percent' Weight(kg)' Percent`s Bay Anchovy 14,977 31.5 15.5 6.9 Lesser Blue Crab 10,502 22.1 48.3. 21.3 White Shrimp 7,245 15.2 43.2 19.0 Brown Shrimp 5,146 10.8 36.1 15.9 Blue Crab 1,93.3 4.1 25.6 11.3 Spot 1,916 .4:0 6.9 3.0 Pink Shrimp 542 1.1 2.1 0.9 Atlantic Croaker 523 1.1 6.3 2.8 Brief Squid 441 0.9 3.7 1.6 Blotched Swimming Crab 422 0.9 1.2 1.6 Other taxa(n=54) 3,937 8.3 38.4 1.6.8 Total 47,583 100.0 227.2 100.0 2023 Taxon Number' Percent' Weight (kg)' Percent& Brown Shrimp 17,33.6. 35.8 60.2 22.5 White Shrimp 8,147 16.8 409 15.3 Bay Anchovy 6,003 12.4 9.4 .3.5 Lesser Blue Crab 3,799 7.9 10.5 3.9 Striped Anchovy 1,538 3:.2 .10.1 3.8 Spot 1,378 2..8 8.3 3..1 Brief Squid 1,370 2.8 6.9 2.6 Atlantic Menhaden 1,298 2.7 30.0 14.9 Pink Shrimp 1,040 2..1 3.2 1.2 Hardback Shrimp 990 2.0 0.8 0.3 Other taxa(n=62) 3,937 8.3 38.4 16.8 Total 48,380 100.0 267.E 100.0 }Numbers and weights are sums of the twelve sampling day totals. "'Percentages may not add up to 100 due to rounding. Water Resources Duke 1EnPrgy Progress,LLC 14 Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Table 2.5 Mean densities(mean no.11000 m3 per sampling day) of selected larval taxa+ entrained and impinged (1-mm fine mesh)at the Brunswick Steam Electric Plant,2022. Month Taxa Jan Feb Mar Apr May Jun Jul AugSep Oct Nov Dec Atlantic Menhaden* Larvae entrained 18 30 33 0 0 0 0 0 0 0 0 ::F-0 Larvae impinged 4.1 4.4 2.8 0 0 0 0 0 0 0 0 D Spot* [Larvae entrained 123 52 95 28 0 Q 0 0 0 0 arvae impinged 14 17 1 7.9 17.2 1 0 0 0 1 0 1 0 1 01 0 1 0 Atlantic Croaker* Larvae entrained 83 0 61 8.6 0 0 0 0 0 78 41 b.2 Larvae.im in ed 24 2.2 5.1 1 6.1 1 0 Pinfish Larvae entrained 11 0 0 11 0 0 0 0 0 1 0 6.9 0 Larvae impinged 1 3.2 1 L6 L 0.6 0.3 01 0 0 1 0 1 0 10 0.1 0 Seatrout* Larvae entrained 0 0 0 0 9.3 3.4 3.1 0 0 0 0 0 Larvae im in ed 0 0 0 0 0.5 0.7 0 0 0 0 0 0 Mullet* Larvae entrained 3.5 14 8.3 0 0 0 Q 0 0 0 0 0 Larvae impinged 0 0 0 0 0 0 0 0 1 0 0 0 0 Silversides Larvae entrained 0 0 31 95 22 0 0 1 0 1 0 0 0Tqo I.Larvae im in ed 0 0 0 1.7 0 0 0 0 0 0 0 Anchoa spp. (?13mm)* Larvae entrained 1 170 1 37 193 463 3.2 9.7 19 16.5 3.6 6.0 Larvae impinged 5.4 5.3 0.2 3.5 2.1 31 0.3 0.2 0 0:3 0.8 0:1 ' Anchoa s <13txim Larvae entrained 0 0 0 101 271 181 19 91 9.5 0 0 0 Larvae impinged 0 0 0 11 0.5 1 47 0 0.2 0 10 0 0 Gobiosoma s eLarvaeeertrained 0 0 0 71 266 461 133 283 85 0 0 0 vm u� cd 0 0 0 0.8 1.3 30 0.5 0.4 1.0 0 0 0 Duke Energy Progress,LLC t5 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report mmvr Table 2.5 (continued) Month Taxa Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Cteno obious/Gobionellus s Larvae entrained 25 0 5.7 31 16 32 0 3.2 9.4 6.6 3.3 0 Larvae irr in ed 2.2 0.3 2.0 8.5 0.8 2.1 0 0.1 0 0 0.9 0.2 Shrimp ostlarvae* Larvae entrained 3.8 F55 218 6.1 22 1 89 1 52 32 93 79 17 8.8 Larvae impinged 27 30 367 117 51 61 2.1 17 68 12 13 0.3 Hardback Shrimp Larvae entrained 0 0 0 0 6.6 0 3.4 0 0 0 0 0 Larvae impinged 1 0.3 0 1 0 0 0 0.3 0 0 0 0 0 0 Portunid crab merato * Larvae entraine 23 0 2.9 0 16 32 3.2 34 30 37 0 Larvae im in e 4.7 15 9.9 11 25 5.0 3.9 l 5 18 23 5.3 Total all or anisms Larvae entrained 452 229 483 497 962 1 1,284 I 270 433 287 226 112 27 Larvae im in ed 88 68 403 180 96 207 28 23 $5 31 46 11 +Selected taxa comprised> m of the total number sampled in either entrainment or larval impingement sampling and/or historically representative important taxa(RT)indicated by an asterisk. Duke Energy Progress,LLLC 16 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Table 2.6 Mean densities (mean no./1000 m3 per sampling day) of selected larval taxa' entrained and impinged (1-mm fine mesh)at the Brunswick Steam Electric Plant,2023. Month Taxa Jan Feb Mar Apr May Sun Jul Aug Sep Oct Nov Dec Atlantic Menhaden* Larvae entrained 6.9 6.8 127 9.3 0 0 0 0 0 0 0 0 Larvae im in ed 4.5 4.5 34 0.8 0 1 0 1 01 0 1 0 0 1 0 0 S ot* Larvae entrained 57 82 42 61 0 0 1 0 0 1 0 0 1 0 0 Larvae impinged 20 41 12 2.9 J OA 0 1 0 1 0 0 1 0 0 1 0 Atlantic Croaker* Larvae entrained 280 311 296 37 3 0 0 0 0 36 138 78 Larvae impinged 206 264 96 2.9 0 0 0 0 0 120 8.3 26 Pinl;zsh Larvae entrained 6.7 7.0 28 12 0 0 0 0 0 0 Larvae impinged 0.5 1.4 2.9 1.9 0 0 0 0 0 0 0 0.3 Seatrout* Larvae entrained 0 0 0 0 8.9 12 50 3.2 0 0 0 Q Larvae im in ed 0 0 0 0 1.9 0.5 5.4 0 0 0 0 0 Mullet* Larvae entrained 0 0 Larvae impinged_ 0.5 0 0 0 0 0 0 0 0 0 0 0 Silversides Larvae entrained 0 0 61 291 15 0 0 0 0 0 0 F 0 Larvae impinged 0 0 0.6 5.3 0.7 0 0 0 0 0 0 1 0 Anchoa s >_13mm '� Larvae entrained 14 6.7 0 0 735 9.0 50 6.3 197 13 3.1 0 Larvae impinged 8.2 2.4 0 0 56 8.7 7.1 0 0 7.9 0.1 0 Anchoa s <I3mm * Larvae entrained 0 1 0 0 56 30 3.0 9.2 58 13 0 0 0 Larvae impinged 0 0 0 13 6.8 0.1 1 1.2 1.3 0 0 J 0 Q Gobiosoma.s .* Larvae entrained Q 1 0 0 6.1 422 241 234 65 161 0 0 0 Larvae im in ed 0.5 0 0 0 39 7.7 9.5 0.3 0.8 3.7 0 0 Oulce Energy Pro tress,LLC 17 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Re ort Table 2.6 (continued) Month Taxa Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Cteno obious/Gobionellus s P. Larvae entrained 3.3 3.5 21 24 42 0 6.4 3.2 32 19 21 0 Larvae im in ed 2.2 0 14 2.7 5.5 2.4 2.5 0 i.0 23 L4 1.0 Shrimp ostlarvae* Larvae entrained 20 44 589 229 39 26 59 43 38 55 52 10 Larvae impinged 20 71 221 45 81 76 13 103 19 76 611 6.8 Hardback Rd Larvae entrained 0 0 0 0 0 0 0 9.5 0 0 0 0 Larvae im in ed 0 0 0 0 0:3 7.3 2.6 3.5 d 18 0 0.3 Portunid crab me alo s* Larvae entrained 63 3.5 12 110 12 6 3.2 0 0 5$ 304:63 Larvae irr► in ed 0.6 2.6 29 43 30 3.8 11 4.0 2.5 66 11 Total all or anisms Earvae arvae entrained 425 478 1,196 937 1,510 344 453 222 4b1 197 249 91 im in ed 268 391 417 125 244 109 b2 115 25 320 83 34 Selected taxa comprised> 1%of the total number sampled in either entrainment or larval impingement sampling and/or historically representative important taxa(RT)indicated by an asterisk. Duke Energy Progress,LLC 18 Wafer Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Table 2.7 Density (no./million m3) and modal length (mm) for representative important. taxa collected by month with juvenile and adult impingement sampling at the Brunswick Steam Electric Plant,2022. Month Taxa Jan Feb Mar Apr May Jun Jul Aug_ Sept Oct Nov Dec Atlantic Menhaden densityl 2.4 L9 Q 0.2 20 1 3.8 1 0 1 0.2 0 L3 0.2 0 modal lenA 95 100 - IN 90 1110,1251 IN I IN IN - IN - Spot densi 19 2.0 2.2 64 253 4.8 11 0.4 0.2 1.3 0 0 modal len t 1 60 65 INJ 40 1 40 IN 55 IN IN IN Atlantic.Croaker densityl 8.0 4.4 12 59 16 2.0 0.4 Q 1.0 0.2 0 0.2 modal ten i 105 40 45 60 40 IN IN - IN 1N - IN Flounder dens it 0.2 0 0 4. E 2.6 0.2 0.4 0modallen th IN - IN IN IN IN - IN IN - Seatrouf densi 0 0 0 0 0 1.6 6.9 0 0 0 0 0 modal ten th - - - - - 70 - ' - - White Shrimp densityl 49 1 30 10 19 6.3 7.7 43 504 90 377 184 2.7 modallen th 100 100 105 115 i15 IN 55 100 50 75,95 75 IN Brown Shrim densitvI 0 1 0 1 3.0 0 219 284 373 28 0 23 0 -0 �--Tnqdal length - 1 40 1 60 90 100 65 - 90 - - Pink Shrimp densi 4.8 0.7 0 20 28 0 0 17 4.2 14 12 0.2 modal length lengthl IN I IN I - 45 110 - - 55 40 65 70,80 IN Blue Crab- Q 6.3 4.2 27 203 10 3.5 32 35 23 9.0 4.2 Lesser Blue Crab" 0 0 3.0 8.1 1,699 125 65 0.2 7:7 2.6 29.4 0 Bay Anchovy" 1 1,012 81 239 1,030 477 W4 63 1 2.3 3.7 5.8 12 4.0 Total organisms 1,3311 140 1 306 1,373 3,064 1 572 729 1 625 1 154 T 458 277 48 Length-frequency analysis was not conducted on Blue Crab,Lesser Blue Crab,and Bay Anchovy data. §IN=insufficient size range collected for determination of modal length. *Summer and Southern Flounder. 'Weakfish and Spotted Seatrout. mm Duke Energy Progress,LLC 19 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report kimondsomm Table 2.8 Density (no./million m3) and modal standard length(mm) for representative important taxa collected by month with juvenile and adult impingement sampling at the Brunswick Steam Electric Plant,2023. Month Taxa Jan Feb Mar Apr May Jun Jul Aug Sept Oct Nov Dec Atlantic Menhaden densitvI 4.5 1 2.2 0 0.4 1 222 1 4.6 6.3 0 0.6 0 0 0.7 modal lent 100 IN - IN J 125 125 105 - 130 - - IN S of density 1.1 0.9 5.1 69 158 8.9 7.9 1.2 4.5 0 0 0 modallen th 1 70 IN 90 40 45 40 170,75 1 110 85 - - - Atlantic Croaker densityl 3.1 8.9 1.2 54 29 P.2 3.5 0 5.4 2.1 1.2 1.5modallen th 120 45 130 60 40N I 1N - IN IN 120 IN Flounder' densi 0 0 0 2.0 0 0 2.2 0.5 0.7 0.4 0 0 modal len t - - - IN - - I IN SeatroutE densityl 0 0 0 0.2 0 0 0 0 0 0 0.2 0 1N White.Shrim densi 55 14 11 3.1 9.6 7.3 161 228 685 97 231 45 F7--m-Odallength 70 75 90 IN 1 115 rN 1 5.0 100 100 100 70 70 Brown Shrim densit 0 0 0 49 1 880 598 1,444 197 21 11 2.3 1.5 modal length - - - IN 1 55 80 85 J 75 155,75 75 IN IN Pink Shrimp densityl 0 17 1.5 7.8 42 5.4 88 27 7.5 3.7 2.3 0 modallen th IN IN - 45 110 - - 35 40 65 70,80 IN Blue Crab- 1.8 0.4 1 13 1 32_1 44 24 10 13 5.2 4.5 4.3 2.8 Lesser Blue Crab- 0 1.3 7.8 32 12 226 320 22 56 4.5 3.0 5.1. Bay Anchovy 138 240 15 S24 176 15 24 0 0 6.4 31 102 Total organisms 212 302 101 1,337 1,710 921 2,355 513 933 149 324 252 -Length-frequency analysis was not conducted on Blue Crab;Lesser Blue Crab,and Bay Anchovy data. §IN=insufficient size range collected for determination of modal length. *Summer and Southern Flounder. EWcakfish and Spotted Seatrout. Duke Energy Progress,LLC 20 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitorin Re ort Table 2.9 Mean percent survival+ and percent of total number of larval organisms collected:during larval impingement sampling at.the Brunswick Steam Electric Plant,2022-2023. Mean percent survival Percent total number Adjusted Intake Intake Taxa Screens Controls Screensl 2022 2023 ShrimpE postlarvae 90 89 100 58.8 38.6 Portunid rnegalops 87 86 1.00 12.0 1.0.6 Anchoa spp.>13 0.7 NC 0.7 4.3 4.7 Atlantic Croaker 34 87 39 3.9 27.7 Spot 29 86. 34 3.4 2.5 Blue crabs 92. 92 100 1.7 0.1 Ctenogobius spp. 15 NC 15 1.5 2.8 Planehead Filefish 70 NC 74 1.1 0 .Atlantic 1.9 Menhaden 3.2 39 8.0 0.8 Flounder spp. 93 97 9.6 0.4 0.0 Pinfish§ 34 87 39 0.4 0.3 Pink and white shrimp 96 93 100 0:2 0.2 Hardback Shrimp 7.9 89 8.9 0.1 1.7 Weakfish 13 54 24 0.1 0.2 Prionotus spp. 90 97 93 <0.1 <0:1 Mullet 70 92 76 0 <0.1. Jacks 36 NC 36 0 0 Percent.total number collected 88.7 91.4 }Mean present survival values include data collected during 4 years of intake screen survival studies conducted.from 1984-1987. Results are presented in CP&L 1087 and 1988 (fast-screen rotation). ISurvival results were adjusted for control mortality when control results were available: An entry of NC for control data indicates that no control data were. collected. Adjusted intake screen survival was estimated by dividing the treatment survival by the control survival to account for competing sources of mortality. The adjusted intake screen survival was truncated to 100 in cases where the calculated value exceeded.100. §The survival percentage for pinfish is an estimate based upon data for.a similar species,croaker. Brown,White; and Pink Shrimp. Dukc Ea7ergy Progress,LLC 21 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Table 2.10 Mean percent survival'and percent total number of organisms collected during impingement sampling at the.Brunswick Steam Electric Plant,2022 2023. Mean percent survival Percent total number Adjusted Intake Intake: Taxa Screens Controls Screensi 2022 2023 Bay Anchovy 4.9 73 6,7 31.5 12.4 Blue crabs 96 92 100 26.2 9.6 Shrimp(pink& white) 94 93 100 18.8 18.9 Brown Shrimp 90 80 100 8.3 35.8 Spot 60 98 61 4.0 8.3 Atlantic Croaker 45 93. 48 1.1 1.2 Portunus spp:§ 96. 92 100 0.9 <0.1 Star Drum§ 45 93 48 0.8 0 Blackcheek Tonguefish 83 98 85 0.8 l.6 Silver Perch§ 60 98 61 0.4 0.6 Atlantic Menhaden 16 67 24 0.3 2.7 Mullet 92 92 1.00 0.1 0.1 Weakfish 35 5.4 65 0.1 <0.1 Flounder 71 97 73 0.1 6.1 Percent total number collected 93.4 91.3 + Mean present survival values include data collected during 4 years of intake screen.survival .studies conducted from 1984-1987. Results are presented in CP&L 1987 and 1988 (fast-screen rotation). Survival results were adjusted for control mortality. Adjusted intake screen survival was estimated by dividing the treatment survival by the control survival.to account.for competing. sources of mortality. The adjusted intake screen survival was truncated to .100 in cases where the calculated value exceeded 100. §Survival percentages for Portznzus spp.,star drum,and silver perch are estimates based upon data for similar species(blue crabs,Atlantic Croaker., Spot,respectively) Dixie Energy Progress,LLC 22 Water Resources Brunswick Steam Electric Plant 2622-2023 Biological Monitoring,Re ort Table 2.11 Mean annual percent reduction in the number of representative taxa entrained and reductions in impingement mortality at the Brunswick Steam Electric Plant, 1984-2023+. Entrainment 31A Impingement Percent Percent reduction reduction Anchovies 80 Bay Anchovy 37 Atlantic Menhaden 61 Atlantic Menhaden 99 Spot 62 Spot 97 Atlantic.Croaker 61 Atlantic Croaker 92 Seatrout 62 Seatrout 92 Mullet 90 Mullet 98 Flounder 92 Flounder 90 Gobiosoma spp 80 ShrimpE postlarvae 82 Shrimp 94 Portunid crab megalops 96 Blue crabs 99 Total organisms 75 'Baseline entrainment and impingement rates for years without reduction controls in place used data from 1979-1982 for entrainment:and 1977-1979., and 1982 for impingement. (Impingement data from 1980 and 49.81 was exclude,from the_baselme calculations since:a temporary fish. diversion structure was in place those years,) e Brown,White, and Pink Shrimp. Duke Energy Progress,LLC 23 water Resotirces Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report 1200 _ 1000 v 800 0 L` 600 m s 400 di m LL 200 0 30 F06 Op" Py" 10 00 3uL PUG SEP OGI 00,4 QEG 2022 2023 Figure 2.2 Mean daily freshwater flow (cros) to the Cape Fear Estuary,2022-2023. 500 q� 400 E v 3 300 0 6. 4" 3 200 s m Li 100 0 'P01 2003 2006 2001 20* 20A1 20,%3 20'%5 0%, 20%, 2021 2p22 Figure 2.3 Annual mean daily freshwater flow to the Cape Fear Estuary,2001-2023. Duke Energy Progress,LLC 24 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report 40 35 30 25 NOOOp- 20 15 10 _ 5 0 0nSaliuity(ppt) —6--Temperature(°C) Figure 2.4 Mean monthly intake canal salinity(ppt)and water temperature(°C)2022. 40 35 - 30 25 20 �.._... 15 10 5 0 FvB o'ksyR �'Q4 ���•; 5��1 SN}� VG Sv UDC '014 'F,C —4o—Salinity(ppt) Temperature(°C) Figure 2.5 Mean monthly intake canal salinity (ppt) and water temperature (°C) 2023. Duke Energy Progress,LLC 25 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report .r 9,000 100 to 8,000 h ��� ^ 80 C 7,000 4 E 6,000 �e� .� I 60 = 5,000 4,000 40 c c � Lu 3,000 v 0 2,000 20 a- M E 1,000 Z 0 0 b �. 0-0 oGOO,, o`n, m m m 0)11.11 0) 0) o 0 0 0 0 0 0 0 0 0 0 , 0 N N N N N N N N N N —�— \mnberentrained Pei-cent reduction Baseline Figure 2.6 Annual number of total organisms entrained at the Brunswick Steam Electric Plant and percent reduction from baseline entrainment estimates, 1979-2023. 900 100 800 d�� tie • 1 /4 I • t Q 80 700 bed { d° /1 It 1 600 1 1 { A. 1 = 60 500 1 I 11 1 � 11 111 � 400 40 = W 300 1 { U 1 200 20 a E 100 Z e 0 N Cf ^ M 0 p^j pOj pOj pCO pOj OOj OOj OOj OOj OOi O O O O O O O O O O O O --�— Number entrained — -A— - Percent reduction Baseline Figure 2.7 Annual number of Spot entrained at the Brunswick Steam Electric Plant and percent reduction from baseline entrainment estimates, 1979-2023. Duke Energy Progress,LLC 26 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report 160 100 c �"� 1 t O 140 ' } 80 O 120 100 I I I I 1 r ,"° 1 I 60 as 80 � I c 60 w 40 40 II 20 IZ 20 1 I n ib a obi rn a o o O O o ^ ^ ^ ^ ^ N N Number entrained Percent reduction Baseline Figure 2.8 Annual number of Atlantic Menhaden entrained at the Brunswick Steam Electric Plant and percent reduction from baseline entrainment estimates, 1979-2023. 1200 A , o� �, .�a� o,ao 100 0 1000 80 c O 800 1A - -- — 60 U m ° c °1 C 600 40 c c a� W 400 t� a� 20 d 200 z 0 ^ 0 O) ^ M h ^ C> ^ M h ^ O� ^ M In f� Of f- fy h n Of p�j per, p�j Obi (7) per, M 0) (7) Obi C. O o O O O O O 14 O O o N N N N N N N N N N N N --_A— Number entrained _ — Percent reduction Baseline Figure 2.9 Annual number of portunid crab megalops entrained at the Brunswick Steam Electric Plant and percent reduction from baseline entrainment estimates, 1979-2023. Duke Energy Progress,LLC 27 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report 600 100 N q 0 500 ��� �� p� 80 C E 400 �° 60 2 300 +r 40 W 200 m a� 20 �- 100 Z 0 ^ M y l� ofM ° n wo c, o, 0rn o� o 0 0 0 o w w w w ^ N N - of � � � N N N N N NV Number entrained — —L4 — Percent reduction Baseline Figure 2.10 Annual number of commercial shrimp postlarve entrained at the Brunswick Steam Electric Plant and percent reduction from baseline entrainment estimates, 1979-2023. 12.0 100 .2 10.0 80 =o 8.0 60 m a� c 6.0 Q 40 E 4.0 v Q 2.0 20 d E ° z 0.0 I� 07 w M h ^ O) ^ M h n OJ ^ M H ^ O- w M h ^ O) w M A h CO ep [ti, C q, O O O Of 01 O O O O ^ w ^ ^ w CVN w Q) N N N N N N nr N N N N N — Number impinged — &— — Percent reduction fish diversion structure Baseline A Percent reduction fish diversion structure and return system Figure 2.11 Annual number of Atlantic Menhaden impinged at the Brunswick Steam Electric Plant and percent reduction from baseline impingement estimates, 1977-2023. Duke Energy Progress,LLC 28 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report 10.0 100 c 9.0 8.0 80 O E 7.0 6.0 60 d = 5.0 C 4.0 40 L 3.0 a 2.0 20 d 1.0 Z 0.0 M 0 0) C) 0O, 0O, pO, 0) C)C) C) M 0) O CO Cr) 05 Oj C) O O O O O O O O O O O CV•- e- �- '- •- �- N N N N N COW N N N N N Number impinged — -A— — Percent reduction fish diversion structure Baseline —A Percent reduction fish diversion structure and return system Figure 2.12 Annual number of Bay Anchovy impinged at the Brunswick Steam Electric Plant and percent reduction from baseline impingement estimates, 1977- 2023. 100 =O 1.4 ,t Aaa�e,�d 4 a 80 p E 1.2 v 1.0 - 60 0.8 Q 40 C E 0.6 v 0.4 20 a E 0.2 =S 0 Z 0.0 MCO CO CO C)+A I� Ol M h per, 05 0) M 0O, 0500 M M M 0Of M 0) O O O O O O O O O NO O O N N — ^--- Number impinged Percent reduction fish diversion structure Baseline ♦ Percent reduction fish diversion structure and return system Figure 2.13 Annual number of Spot impinged at the Brunswick Steam Electric Plant and percent reduction from baseline impingement estimates, 1977-2023. Duke Energy Progress,LLC 29 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological Monitoring'Report MMMM 4.0 100 0 3.5 80 r 3.0 0 E dl -0 2.5 1 60 2.0 11 R Ij �1 W _ ! 1 Q' 1.5 s� � ;, � 1 i 40 1 11 1 11 1 '1 V 1.0A 1 1 1 1 1 1 1 1 11& 20 d E 0.5 0 Z 0.0 O ^ M 0) C)) 00) C) 00, p0, O0, QOj 0)j M � O O O O O O O O O O O O e- ^ ^ ^ ^ ^ N N N N N N N N N N N N —� — Number impinged — -A— — Percent reduction fish diversion structure Baseline ♦ Percent reduction fish diversion structure and return system Figure 2.14 Annual number of commercial shrimp impinged at the Brunswick Steam Electric Plant and percent reduction from baseline impingement estimates, 1977-2023. 1.5 Vf 100 R c � «♦ � � P'� •to e.� 1 Aa �; `/ ` ; A l � i i 1 1 80 c k 14 d��s>, i i ` ' !� i 1 L) 1.0 60 tM c 40 .r 'Q c E 0.5 CD 11 11 20 W 11 11 a E i z 0.0 0 NO ^ M h !� O) ^ M 47 I� O ^ M N /� O ^ M a, cb ty CO CO o� o� o� rn rn O O o 0 0 ^ ^ ^ ^ ^ N N � ^f 0) Rf N N N N N N N N N N N N - Number impinged — -A— — Percent reduction fish diversion structure Baseline ♦ Percent reduction fish diversion structure and return system Figure 2.15 Annual number of blue crabs (all Callinectes spp. combined) impinged at the Brunswick Steam Electric Plant and percent reduction from baseline impingement estimates, 1977-2023. Duke Energy Progress,LLC 30 Water Resources Brunswick Stearn Electric.Plant 2022-2023 Biological Monitoring Re ort 3.0 REFERENCES Blumberg, A. F., D. 7. Dunning, H. Li, D.Heimbugh, and W.R. Geyer. 2004. Use of a particle tracking model for predicting entrainment at power plants on the Hudson River.Estuaries.27 (3): 51.5 -526. CP&L. .1980..Brunswick Steam Electric Plant,.Cape Fear Studies Interpretive Report, Carolina. Power&Light Company,New Hill,NC. .1982. Brunswick Steam Electric Plant annual biological monitoring report, .198.1. Vol I. Carolina Power&Light Company,New Hill,NC. .1984. Brunswick Steam Electric Plant annual biological monitoring report, 1983. Carolina Power&Light Company,New Hill.,NC. .1985a. Brunswick Steam Electric Plant annual biological monitoring report, 1984. Carolina Power&Light Company,New Hill,,NC. . 1985b. Brunswick Steam Electric Plant Cape Fear Studies, Interpretive Report, Carolina Power&.Light Company,New Hill,NC. _. 1986. Brunswick Steam Electric Plant annual biological.monitoring report, 1.985. Carolina Power&Light Company.,New Hill,NC. _. 1987. Brunswick Stearn Electric Plant annual biological monitoring report, 1986. Carolina Power&Light Company,New Hill,NC. . 1988. Brunswick Steam Electric Plant annual biological monitoring report; 1987. Carolina ^Power&Light Company,New Hill,NC: 1989. Brunswick Steam Electric Plant.-annual biological.monitoring report, 1988. Carolina Power.&Light Company, Southport,NC. .2002. Brunswick Steam Electric.Plant annual biological monitoring report, 2001.. Carolina. Power&.Light Company,New Hill,NC. Copeland,B. J., R. G. Hodson, and R.I Monroe. 1979.. Larvae and.postlarvae in the Cape Fear Estuary,North Carolina,during operation of the Brunswick Steam Electric Plant, 1974-1978. North Carolina.State University;.Raleigh,NC. DEP: 2017. Brunswick Steam Electric Plant annual biological monitoring report, 200-2015. Duke.Energy Progress,LLC,New Hill,NC. . 2019. Brunswick.Steam Electric Plant annual biological monitoring report, 2016. Duke uEnergy Progress;LLC,New Hill,NC. Duke Energy Progress,LLC 31 Water Resources Bruns,snick Steam Electric Plant 2022-2023 Biological Monitoring Re ort .2020. Brunswick Steam Electric Plant annual biological monitoring report,20I 7-2018. Duke Energy Progress,LLC,New Hill,NC. _. 2021.Brunswick Steam Electric Plant annual biological monitoring report, 2019. Duke Energy Progress,LLC,.New Hill,NC. .2023. Brunswick:Steam Electric Plant annual biological monitoring report,2020-2021. Duke �Energy.Progress,LLC,New Hill,NC. Giese,G.L.,.H.B.Wilder, and G. G.Parker,Jr. 1979. Hydrology of major estuaries and sounds of North Carolina. United. States Geological Survey. Water resources investigations 79-46. Raleigh,NC. 1985.Hydrology of major estuaries and sounds of North Carolina. United States Geological. Survey. Water-supply paper 2221. Alexandria,Va. Hogarth, W. T. and K.L.Nich.ols. 1981. Brunswick Steam Electric Plant intake modifications to reduce entrainment and impingement losses. Carolina Power. & Light.Company, New Hill, NC: Lawler, J. P., M. P. Weinstein, H. Y. Chen and T. L. Englert. 1988. Modeling the physicat and behavioral mechanisms influencing the recruitment of spot and Atlantic croaker to.the Cape Fear Estuary..Am.Fish. Soc. Sym.3: 115-131. Nelson, W..R.,M. C. Ingham, and W.E. Shaaf. 1977. Larval transport a..nd year class strength of Atlantic menhaden,.Brevoortia:tyrannus.Fish.Bull. U. S.75: 23-42. Norcross B;L., and FI.M.Austin. 1.981. Climate scale environmental factors affecting year-class fluctuations of Chesapeake Bay croaker,.Micropogonias undulatus. Va..Inst. Mar. Sci. Spec. Sci.Rep. 110: 87 pp: Norcross B. L., and R. F. Shaw. 1984. Oceanic and estuarine:transport of fish eggs and larvae: a review.Tran. Am. Fish. Soc. 113: 1534.65. Rogers.S. E.., T. E. Targett, and S. B. Van Sant. .1984: Fish nursery use in Georgia salt-marsh estuaries:The influence of springtime freshwater conditions.Tran..Am..Fish. Soc. 11.3: 595-606. Schwartz, F.J., P.Perschbacher, L:.Davidson, C. Simpson,D. Mason, M. McAdams,K. Sandoy and J. Duncan. 1979. An ecological study.of fishes and invertebrate macrofauna utilizing the Cape Fear River Estuary;Carolina Beach Inlet,and adjacent Atlantic Ocean, 1973-1977.BSEP Cape Fear Studies,Volume XIV. Report.to Carolina Power&Light Co.;Institute.of Marine Sciences,University of North Carolina,Morehead City,..NC, Thompson, T. E. 1989. Factors limiting the movement of spot, Leiostomus :xanthurus, into a freshwater-oligohaline tidal marsh. Master's thesis. Department of Biological. Sciences, University of North Carolina at Wilmington,.Wilmington,N.C. Duke Energy Progress,LLC 32 NA-titer Resources Brunswick Steam Electric Plant 2022-2023 Biolouical Monitorin Re)ort United States Environmental Protection Agency (USEPA). 2014. National Pollution Discharge Elimination System-Final regulations to establish requirements for cooling water intake structures at existing facilities and amend requirements at phase l facilities; Final rule, 79 Federal Register,pp 48,300-48,439. Weinstein,M.P. .1979. Shallow marsh habitats as primary nurseries for fishes and shellfishes, Cape fear River,North Carolina.Fish. Bull. 77:339-357, Weinstein,M.P. 1979. Larval retention study,,.Cape Fear River, 1978. BSEP Cape Fear studies,Volume X. Report to Carolina.Power&Light Company. Lawler,Matusky & Skelly Engineers,Pearl River,NY. Weinstein,M. P.,:S.L. Weiss,R. G.Hodson,and L..R. Gerry. 1979. Retention of three taxa of postlarval fishes in an intensively flushed tidal estuary, Cape Fear River;North Carolina. Fish. Bull. 78(2):419-436. Weinstein,M.P., S.L.Weiss;and M.E. Walters. 19810. Multiple determinants of community structure in shallow marsh habitats;.Cape Fear River estuary,North Carolina. Mar. Biol.. 58:226-243. Mike Energy Progress,LLC 33 Water Resources Brunswick Steam Electric Plant 2022-2023 Biological MonitoringRe ort APPENDICES Appendix 1. Summary of historical environmental studies conducted in association with. the Brunswick Steam Electric Plant. As part:of the initial nuclear licensing efforts Carolina Power&Light Company(CP&L,. predecessor to Progress Energy Carolinas.,Inc and subsequently Duke Energy Progress,LLC) commenced environmental impact studies in 1968.PhysicaUchemical studies included dye and, flow studies,water chemistry,and thermal studies.Biological studies included phytoplankton,. zooplankton,benthos and larval fish and shellfish diversity,distribution and abundance studies in the main.stem of the estuary.Juvenile and adult fish and shellfish population studies were also conducted in the estuary. Special emphasis was placed on Walden Creek and Snow's Marsh adjacent to the plant's intake canal.Entrainment and impingement studies were also conducted after Unit 2 came online in 1974.In.addition,.thermal tolerance, swimming performance.studies and marsh productivity studies were conducted. Studies were expanded and intensified in 1976 to,address the potential.adverse impact on the entire estuary by the intake under full 2-unit operation.These comprehensive studies were developed through an.interagency review group assembled at CP&L's initiative and included representatives of the EPA,NRC,.National Marine Fisheries Service,.United States Fish and Wildlife Service,North Carolina Division of Marine Fisheries;North Carolina Division.of Environmental Management:(predecessor to NC DENR),.and CP&L.Exhaustive dye tracer, hydrographic,tidal,temperature,and salinity studies were conducted to model estuarine flow dynamics relative to plant intake flows.Various gear efficiency and sampling methodology .studies were conducted to ensure that.statistically valid data were collected.The biological studies were modified to provide.adequate data concerning the.larval,juvenile;and adult.fish and shellfish populations having the greatest potential to be.adversely affected by plant operation.In addition to weekly entrainment and impingement studies.,population studies were conducted in the nearshore ocean,main stem estuary,estuarine shallows, and tidal creek nursery areas from below Southport,NC upstream to the vicinity of Wilmington,NC. Specific criteria studied included species composition plus spatial,seasonal,and inter-annual abundance as well as age, growth,dietary, and tagging studies:The principal investigators from CP&L,NCSU,.Institute of Marine Science and.Lawler,Matusky and Skelly consultants used results of these studies to determine the mechanisms by which larvae enter and are retained in.this intensiv..ely flushed estuary and to provide insight regarding the lack of any detectable adverse environmental impact on .fish and shellfish populations due to cooling water withdrawal.Results of these studies were compiled in the 20 volume.Cape.Fear Studies. Beginning in 1980 and continuing through 1.993,CP&L conducted a long-.term Biological.Monitoring Program that would provide for the continued assessment of the impact that the cooling water withdrawal might have:on the Cape Fear River Estuary.Using historic biological study results as a guide,particular emphasis was placed on marine fisheries. With some rhodification,these Biological.Monitoring Studies were a continuation of the studies conducted since 1976,thus,allowing for long-term trending of results. Certain studies were also expanded or added to address the effectiveness of the circa 1981 intake modifications in reducing the number of organisms entrained and impinged. Beginning in 1984, a larval impingement study was conducted in addition to the standard entrainment and impingement.studies.as Duke Energy Progress, LLC A-1 Water Resources Unit Brunswick Steam Electric Plant 2022-2023 Biolo ical Monitoring R-eg ort Appendix 1. continued. another means to assess the success of fine-mesh screens in reducing.entrainment of larvae. Fine-mesh screens and the fish return system were completed during.June of 1983..Data prior to 1983 can.be used to estimate annual numbers entrained during a period with no controls in place (i.e., no continuously rotating fine-mesh screens or.fish return system). Mean daily entrainment densities were multiplied by design cooling water flow and summed to obtain an annual estimate of total number entrained.The design flow of 84.15 cubic meters per second(curs) is the rated flow of all retain cooling water pumps per unit.Linear interpolation was used to estimate entrainment rates on non-sampling days. Annual numbers entrained after 1983 were calculated in a similar fashion with the exception that NPDES permitted cooling-water flow(i.e.,reduced flow) was used. A calculated annual baseline entrainment.rate was obtained by averaging the annual number entrained for the years 1979 through 1982. The estimated annual number entrained each: year after.1983 was subtracted from the annual baseline estimate to obtain a percentage reduction from baseline due to the installation offrne=mesh screens,.fish,return system,and reduced cooling- water flow. A similar process allowed forthe calculation of annual numbers impinged for comparison to a.calculated baseline annual impingement rate. Two additional years of data, 1977 and 1978, were recovered from historical data sets for inclusion with the long-terrn comparisons.The period 1977-1982 represents a period without impingement .controls in place. (i.e., no fish diversion. structure,fish return system,or flow reductions). The calculated baseline impingement rates were obtained by-averaging the annual number impinged from 1977-1982 with the exception that data from 1980.and. 1981 were not used in the baseline:.calculation since a:temporary fish diversion structure was in place during that time. The estimated:annual number impinged each.year after 1983 was subtracted from the annual baseline estimate to obtain a percentage reduction from baseline due to the installation of the fish diversion structure, fish return.system, and reduced cooling-water flow. Impingement sampling only occurred during the last 6. months of 1983. because construction.of the fish return system was in progress earlier that year. Subsequently, results of impingement sampling during 1983 .are presented but not included in the percentage. reduction calculations. The inclusion of`survival data.allowed for an assessment of reductions in impingement mortality due. to the fish diversion structure and the return.system together for different species. Survival in the return system was determined for selected size classes of the dominant organisms.that have been impinged at the BSEP in past years(CP&L 19.85a, 1986, 1.987, 1.988). Studies conducted from 1984-1987 were designed to assess survival of larger organisms impinged on the traditional traveling screens and survival of the entrainable size classes of organisms impinged on 1-mm fine mesh.Weekly survival studies were conducted initially.The frequency of studies was reduced to the.peak periods of abundance for selected species and size class during the latter two years of studies.to target species and size classes where data was. lacking. Acute and latent 96-hour survival was assessed. Results from the .four-years of survival studies are summarized in the 1987 Biological.Monitoring Report(CP&L 1988). Larval fish sampling was conducted in the ship channel and the mouths of two major tidal creels.nursery areas. A larval fish discrete depth sampling study was conducted to better Water Resources Unit Duke Energy Progress, LLC A-2 Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report understand the distribution of larvae during recruitment to the estuary and to.validate the standard larval fish study. A marsh nursery study using trawl,seine,and rotenone sampling was conducted to assess recruitment to and use of the tidal creek nursery areas with special interest placed on Walden Creek adjacent to the Plant's intake.canal and several nursery areas upstream of the intake canal. Sampling stations also included a location in the fish return basin, This station along with a special tagging study demonstrated that juvenile fish and shellfish used the return basin as .a shallow water nursery area in a fashion similar to the headwaters of a natural tidal creek. A nekton study using trawl and gill.net sampling was conducted to assess the populations of juvenile and adult fsh and shellfish that could potentially be subject to impingement at the plant intake structure... Sampling stations .in the CFE ranged from below Southport, NC to the vicinity of Wilmington,NC. Several sampling stations in the.intake canal.were used as an additional means of assessing the success of the fish diversion structure in reducing impingement. A weekly temperature and salinity study was conducted along the main stein of the.estuary because historic. results indicated that freshwater flow,salinity,and temperature were the main determinants of fish and shellfish distribution and abundance in the estuary. Beginning.in 1994 and continuing through the current:permit cycle,the biological monitoring program was significantly reduced with the concurrence of State regulatory and resource agencies. Because of almost two decades of data demonstrating that the cooling water withdrawal has no adverse impact-to the fish and shellfish populations in the estuary,:the monitoring program was reduced to concentrate on entrainment and impingement only. This allowance:was based on the.fact that if the impingement mortality and entrainment performance remain relatively consistent then the environment.remains protected. Sampling frequency was reduced to monthly sampling and results demonstrated the continuing.effectiveness of the intake. modifications in.reducing.entrainment:and.impingement of fish and shellfish. Periodic special study trawl surveys conducted in the marsh/tidal creek nursery areas continued to show no adverse impact on the recruitment.to and.use of these nursery areas by juvenile fish and shellfish. Duke Energy Progress,LLC believes over four decades of biological and environmental monitoring has.resulted in one of the most extensive and informative data bases concerning our nation's estuaries. These data can provide valuable insight regarding the ecology of our nation's estuaries and facilitate compliance.The USEPA published a revised rule to implement§316(b). of the Clean Water Act in the Federal register on August 15,2014(USEPA 20.14). Based.on the evaluation of the 316(b)study reports.submitted in 2022 NCDEQ concluded that the existing configuration at Brunswick represents best technologies available(PTA)for.meeting the impingement and entrainment requirements of the 316(b)rule. Duke Energy Progress, LLC A-3 Water Resources Unit Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Appendix I Total number+of larval organisms collected during entrainment(BE) and larval impingement (LI) sampling at the Brunswick Steam Electric Plant,. 2022. 2022 Scientific name Common name BE LI Anguillidae Freshtivatcr teas Anguilla rostrate American Eel 1 0 Elopidae Tarpons Elops sattr us Ladyfish(lepto.) 3 10 Opbichthidae Snake eels Myrophis pk(netatus Speckled Worm Eel 0 11 .Myrophisptmclatus(lepto.) Speckled Worm Eel QeRto_) 6 7 Clupeldae Herrings Brevoortia tyrannus Atlantic Menhaden 27 54 Clupea harengus Herring 0 0 Engrautidne. Anchovies. Anchoa<13nim Anchoa-13mm 217 231 Anchoa?13mm Anchoa'>_13mm 30S 167 Syngnathidae Pipefrsbes Syngnathus.spp. Pipefish. 1 Atherinidae. Silversides Atherinidae Silversides 49 3 Gerreidae NTojorras Gerreidae Morjarras 10 5 Haemulidne Grunts Orthopristis chrysoplera Pigfish 16 30 Sciaenidae Drums Bainfiella clnysounT Silver Perch 38 19 Cynoscion nebulosus Spotted Seatrout 1 0 Cynoscion vegalis Weakfish 4 4 Lerostomus xanduaus Spot 96 169 Pogonias cronds Black Drum 1 0 Micropogonias undulates Atlantic Croaker 86 287 Sciaenops ocellattrs Red Drum 3 2 Stellffer lanceolatus Star Drum 0 3 Menticirrhus spp. Kingfish 2 S Gobiesocidae Clingfishes Goblesox sinrnro3ns Skilletfish 4 0 Sparidae Porgies Lagodon rhomboids Pinfish 9 21 Archosargusprobatoceplurlus Sheepshead 0 0 Carangidne .Tacks Carangidae lacks. 1 0 Mugilidae Mullets Mugil curer) White Mullet 0 0 Mugil cephalus Striped Mullet 9 0 Blenulidae Combtooth blennies Blennidae Combtooth blennies 14 1 ETeotridne Sleepers Dormhator macidauts Fat Steeper 1 0 Gobiidae Gobies Gobiosoma spp: 411 Hs Microgobitrs spp. S 3 Cienogobitrs/Gobttelhts.spp. 42 65 w Bothidae Let'teye flounders Paralichthys spp. Flounder Unid, 1 7 Citharichthys spp. Whiffs 3 11 Duke Energy Progress, LLC A-4 Water Resources Unit Brunswicic Steam Electric Plant 2022-2023 Biolo-,ical':V'lnnitorinr Report Appendix 2.continued 2022 Scientific name Common name BE LI Soleidae Soles Trinecles maculatus Hogchoker 1 5 Cynoglossidae Tonguefishes Symphrmts spp. 1 0 Herniramphus Ilalfbeaks Hemirantphus spp! 0 0 Synodontidae Liza rdrrsbes Synodus foetens Inshore Lizardfish 0 0 Ephippidne Spadefishes Chaewdipterusfaber Atlantic Spadefish 0 0 Triglidae Searobins Priononts spp: Searobin I 1 Stromateidae Butterfisbes Peprilus triacantlius Butterfish 1 0 Lutjanidae Snappers LuYanrtsgriseut- Gray.Snapper 1 0 Tetraodooitadae Pufferfish Sphoeroides maculatus Northern Puffer I I Monacanthidae Filefish Slephanolepls hispidus Planehead Filefish 1 49 Invertebrates Penaeidae Prawns Commercial shrimp"(postlarve) Shrimp larvae. 241 2,723 Trachypenaeus.constrictus Hardback Shrimp 8 2 Penaeus segerus White Shrimp 0 4 Crangon crattgon Brown Shrimp 0 0 Pandalus borealis Pink Shrimp 0 0 Portunidae Swimming crabs Portunid crab megalops 58 814 Portunid crab?IOmm 3 71 Calllnectes spp. B Inc.Crab:.(>IOmm S20mm) 0 1 Limulidae Horseshoe crabs Limuhts polyphenrus Atlantic horseshoe Crab 0 I '"Numbers represent total number collected(per 2-minute LI sample/per 5-minute BE sample)summed over all samples. "Commercial shrimp refer to White,Brown;and Pink Shrimp of genus.Farfantepenaeus and Litopenaeus. Duke Energy Progress, LLC A-5 Water Resources Unit Brunswick Steam Electric.Plant 2022-2023 Biological Monitorin„Report Appendix 3. Total number' and biomass (g) of juvenile and adult organisms collected during impingement sampling at the Brunswick Steam Electric Plant,2022. 2022 Scientific name Common name Total number I Total weight(g) Dasyatidae whiptail Stingrays Dasyatis Sabina Atlantic Stingray 4 925 Gymouridae Butterfly Ray Gyninura odor Lira Smooth Butterfly Ray 1 190 0 Gy Butterfly mn ura spp. y Ray Rhinopteridne Cownose hays Ainopiera bonasres Cownose Ray 2 1526 Anguillidne Freshwater eels Ang ffla rosirate American Eel 0 0 Ophichthidae Snake eels Myrophis prmctatus Speckled Worm Eel 1 8 Ophichthus go»tesh Shrimp Eel 1 128 Clupcidae Herrings Alosa aestivalis Blueback Herring 5 Beevoortta tyramtus Atlantic.Menhaden 35 1;279 Dorosoma cepedianian Gizzard Shad 0 0 Doroswna petene»se Threadfin Shad 10 58 Opistlronmia oglinum Atlantic`thread Herring 5 40 Alosa sapidisst»ur American Shad 0 0 Rachycentridae Cobia Rachycentron canadum Cobia 0 0 Centrarchidae Centropristis philadelphica, Rock Sea Bass. 0 0 Engraulidae Anchovies Anchoa hepselus Striped Anchovy d4 280 Anchoa thitchilli Bay Anchovy 1,640 1,677 Synodontidae Lizardfshcs Synodusfoetens Inshore Lizardfishes 0 0 Gadidae. Codfishes UrophycisJlorldana Sou tliem Hake 0 0 Vrophycisregla Spotted Hake 9 98 ophidiidae Cusk-eels Ophidlorrtvelshi Crested Cusk-Eel 3 16 Ophidion marginatum Striped Cusk-Eel 0 G Butracbeldidne Toadfishes Opsanus tau Oyster Toadfish 3 9 l8 Gobiesocidae CHinfishes Gobiesox smenrosus Skilletfish 5 16 Cyprinodontidae. Killifishes F;tndnlus heteroclitus Mummichog 0 0 Fimdttlus majalis Striped Killifish. 0 0 Atherinidne. Silversides Membras martinica Rough Silverside 59 145 Menidia menidia Atlantic Silverside 4 10 Menidia beryllina Inland Silverside 0 0 Hemiramphidae Halfbcak Hyporluuirphus'untfasciatus Silversuipe Haltbeak I I Syngnathidae Pipefishes S)mgnathrtsfuscrts Northern Pipefish 26 81 Syngnadurs louisianae Chain Pipefisb. 17 27 Hippocampus erectus Lined.Seahorse 1 2 Duke Energy Progress, LLC A-6 Water Resources Unit Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Appendix 3. continued 2022 Scientific name Common name Total number Total weight(g) Trigliade Searobins prionotusscMdus Leopard S.earobin 4 8 53 411 PrionotusMbidus Bighead Searobin Prionotus mrolinits Northern Searobin 0 0 Lutjauidae Snappers. Lutjanus.griseas Gray Snapper 0 0 Hemiramphus Halfbeaks Heniirantphia spp. © 0 Lepisosteidne Gars Lepisosteus osseus. Longnose.Gar Q Q Gerreidae Mojarras Alapteras:auratus Irish Pompano 0 0 Ertcinostourus argenteus Spotlin.Mojarra o 0 Hacmulidae Grunts Orthopristis chrysoptera Pigfish 0 0 Pomatomidne Bluefishes Pomatonnts saltahix Bluefish I5 94 Carangidne belts Cara=hippos Crevalle Jack 0 0. Selene votner Lookdovvn 64 0 0 Oligoplites saiaw Leathepacket fish 0 achmotus.carolinus Florida Pompano 0 0 Tr Chlorosrombrus citrysunts AtlanticBumper 0 0 Belonidae Needlefishes Strongylura marina Atlantic.Needleftsh 1 3 Sparidae Purgics Archosargus probactocephaliis Sheepshead 1 4 Lagodon rhombodies Pinfish 2 l8 Sciaenidne Drams Barldiella chrysoura Silver Perch 22 $0 Cynoscion nebulosus Spotted Seatrout 0 0 28 Cynoscion regalis Weakfish b 87 Leiostonnts xanthtn-its Spot 0 184 8 Menifcirrints:littoralis Gulf Kingfish828 0 0 Micropogonias undulates Atlantic Croaker 93 1,152 Stellifer lanceolatus Star.Drum 0 rum 59 Menlicirrhus americantts Southern KiWish 0 0 Ephippidae Spadefishes Chaetodipterus faber AtImitic Spadefish l 756 Mugilidae Mullets Mugil cephaltts Striped Mullet 17 719 Bleuniidae Conmtooth blennies Hypsobletrnius hen& Feather Blenny 1 22 Hypsoblenniusionihas Freckled Blenny 1 2 Chasniodes bosquiamts Striped Blenny 1 3 Hypleurochilus gendnalus Crested Blenny I 6 Trichiuridae Snake:mackerels Trichiurus leptunts Atlantic Cutlassfish 21 IN Lefteve flounders 27 Ancylop 1 1 seua quadrocellata. Ocellated flounder 11 1 Citharichthys spilopterus. Bay Whiff Eiro us crossotus Fringed flounder 10 8 99 P- 12 1,319 Paralichihys lerhosagma Southern flounder Duke Energy Progress, LLC A-7 Water Resources Unit Brunswick Steam Electric Plant 2022-2023 Biolozical Monitoring Report Appendix 3. continued 2022 Scientific name Common name Total number Total weight(g) Cynoglossidae. Touogefishes Syrnphumv plagiusa Blackcheek Toungefish 46 298 Balistidae Triggerfishes/Filesfishcs Monacantlutshispidus Planehead Filefish ... Tetraodontidne Puffers Chiloniycterus schoepf Striped Burnish 43 6 40 Sphoeroides maculates Norther Puffer 1 24 Lagocephalrrs laevigants Smooth Puffer € 0 Sphyraenidae Barracudas Sphyraena mrid Barracuda sp. 1 2 Stromateidne Butterfishes Peprilus alepidotus Harvesttish '2 1 4 93 .Peprilus trlacanihus Butterfish 1 4 Scophtbalmidae Turbots Scophthalmtrsaquostrs Windowpane Flounder 3 212 Soleidae Soles Twnectes macrdatus Hogehoker 1 8Ci Invertebrates Loliginidne Pencil squids. Lilliguncula brevis Atlantic Brief Squid 156 841 Squillidae Mantis shrimps Squillaetnpusa Mantis Shrimp 12 364 Penaeidae. Prawm Farfantepenaeus astecus Brown Shrimp 879 7,680 FarJantepenaeus duorarum Pink Shrimp 1,458 5 Litopenaeus serifenrs White.Shrimp ,431 9,851 Trachypeneus constrictus Hardback Shrimp 31 25 Partunidae Swimming crabs T 4 93 CaUinectes sapidus Blue.Crab 9 12,1 Callinectessimills Lesser Blue.Cmb 4104 Callinectes.Spp. Swimming crabs 7 102 Portunm spinimanus Blotch 1 ed Swimming Crab 59 2 Porttrnus:gibbesh Iridescent Swimming Crab 16 28 Numbers.and biomass represent total number and cveight collected per 24-hour sample summed over all sample.dates. Duke Energy Progress, LLC A-8 Water Resources Unit BrunS`vicic Steam Electric Plant 2022-2023 Biological Monitoring Report im n� Appendix 4. Total number+of larval organisms collected during entrainment(BE)and larval impingement (LI) sampling at the Brunswick Steam Electric Plant, 2023. 2023 Scientific name Common name BE L1 Anguillldae. Fresbtivater eels Anguilla rostrate American Eel 0 0 Elopidae Tarpons Elops.saunts Ladyfish.(lepto.) 1 7 Ophichthidae. Snake eels Myroplus punciatus Speckled Worm Eel 2 3 Myrophis ptmetalus(lepto.) Speckled Worm Eel(lepto.) 9 26 Clupeldae Herrings Brevoorda tyrannus Atlantic Menhaden 49 246 Clupea harengus Herring 2 Engraulidae Anchovies Anchoa<13mm Anchoa<13rnm S4 75 Anchoa?13rrm Anchoa>13mm 341 267 Syngoathidae Pipefkshes Symgnathus.spp. Pipefish 3 13 Syngnathus jtscus Northam Pipefish 1 I Syngnathus louisianae Chain Pipefish 0 4 Atherinidae Silvers€des Atherinidae Silversides 120 25 Gerre€dne Mojarras Gerreidee Modarras 4 2 Haemulidac Grunts 0rihoprisus chrysoptem Pinfsh 38 68 Scinenidae Drunks Bairdiella chrysoura Silver Perch 38 .41 Cyhoscion Kebulosus Spotte&Seatrout 20 14 Cynoscion regalfs Weakfish 4 14 Lelostomus xonthitrtts Spot 75 255 Pogonlas cronds Black Drum 2 0 Micropogoulas undulates Atlantic.Croaker 366 2203 Sciaenops ocellatus Red Drum 1 4 SlelWer lanceolattts Star Drum 6 I Menticirrhus spp. Kingfish 0 3 Goblesocidae Clingfishes Gobiesox suvmosus Skillet fish 9 0 Sparidae Porgies Lagodon rhomboids Pinfish 18 33 Archosargus probatocepholus Sheepshead 0 1 Carangidae Jacks Carangidae .lacks 1 0 Mugilidae Mullets Mugil citreina White Mullet 2 0 hfugil cepM- Striped:Mullet 0 1 Bleuniidae Combtooth blennles Blennidae Combtoothblennics 26 2 Eleotridae Sleepers Dormitator macidalus Fat Sleeper 1 2 Gobndae Gobies Gobiosonta.spp. 376 212 Microgobius.spp. 9 2 CtenogobiuslGoblonelhts spp. 57 208 Gobisomd bosc Naked Goby I Duke Energy Progress, LLC A-9 Water Resources Unit Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Appendix 4. continued 2023 Scientific name Common name BE Ll Bothidne Lefteye flounders Paralichthys spp. flounder Unid. & 13 Soleidae Soles Trinectes nrpctdatus. Hogchoker 4 8 Cynoglossidne. Tonguefrshes Syt!phunts spp: 1 I Hemiramphus Halfbeaks Hemiramphus spp. 1 0 Synodontidae. Lizardfishes: Synodusfoetens. Inshore Lizardfish 1 0 Magalopidae Tarpons. Megalops(leptocephalns) Tarpon(larva) I Ephippidae Spadefshes Chaetodiptentsfaber Atlantic Spadefish 0 1 Paralichthyidae. Large Tooth Flounders Ciihorichihys spp. Whiffs 0 7 Triglida.e Searobins Prionomus spp: Searobin 0 3 Invertebrates Penacidae Prawns Commercial.shrimp'(pwtlarve) Shrimp larvae 393 2,559 Trachypenaeus conshictats Hardback Shrimp 3 40 Penaeus setiferra White Shrimp 0 8 Crangon crangon. Brown Shrimp 0 3 Pandalus borealis Pink Shrimp 0 Portuoidae Swimming crabs Portunid crab megalops 78 620 Portunid crab_1Onim. 4 41 CalRnectes.spp. Blue Crab(>10mm:20mm) 0 4 Lilnulidae. Horseshoe crabs Linntitts polyphemus Atlantic Horseshoe Crab 1 0 Loliginidne Squids Lolliguncula brevis Brief Squid 1 0 'Numbers represent total number collected(per 2-minute:LI sample f per 5-minute BE sample)summed over all.samples. "Commercial shrimp referto White,Brown,and Pink Shrimp of genus Farfantependeus and Lilopenaeus. Drake Energy Progress, LLC A-10 Water Resources Unit Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Re ort ti Appendix 5. Total number+ and biomass (g) of juvenile and adult organisms collected during impingement sampling at the Brunswick Steam Electric Plant,2023. 2023 Total number I Total weight(g) Scientific name Common name Dasyatidae Whiptail Stingrays DasyaifsSabina Atlantic Stingray 3 620 Gymnuridae Butterfly Ray Gymmnura micnira Smooth.Butterfly Ray 3 442 Gynrn;aa spp. Butterfly Ray 1 130 Rhinapteridae Cownose Rays Rhinoptera.bonasus Cownose Ray 0 0 Anguillidne Freshwater eels Anguilla rostrale American Eel 2 126 Ophiclithidne Snake eels Myrophis punctatus Speckled Worm Eel 2 6 Ophichthus goniesii Shrimp Eel Ci 1,036 Clupeida a Herrings Alosa aestiralis Blueback Herring I 6 Brevoortia'tyrannus Atlantic Menhaden 164 5,(}96 Dorosonra cepediantau Gizzard Shad I Dorosonra petenense Threadfin Shad 20 76 Opisthoneina oglinuin Atlantic Thread Herring, 48 250. Alosa.sapidissima American Shad I to Rachycenti.idae Cobia Rachycentron canadrrtn Coiiia 0 0 Centrnrchidae Cenitropr7stis philadelphtea Rock Sea Bass 1 65 Engraulidne Anebovies Anchoa.hepsetus Striped Anchovy 22> 1, 83 3 Anchoa mitchilli Bay.Ancbovy 1,271 1,5 Synodontidae Liznvdfishes Synodus foetens Inshore.Lizardflshes 3 381 Gadidae Codfishes Urophycis,Jloridana Southern Hake 0 0 Urophycis regia Spotted Hake 8 285 Ophidiidae Cusk-eels Ophidion ipelshi Crested Cusk-Eel 0 0 Ophidion rnarginatran Striped Cusk-Eel I 8 Battachoididse Toadfishes Opsanus tau Oyster-Toadfish 1 S Gobiesocidae Clingfishes Gobiesox stnanosrrs Skilletfish 1 6 Cyprinodontidae Killifishes Fun&dus heteroclrtus Mummiehog 0 0 Fmrdtibts majalts Striped Killifish 0 0 Atherinidae Silversides Mentbras nrartinica Rough Silverside 49 160 Menidia nienidia Atlantic Silverside 12 42 Menidia beryllina Inland Silverside 0 0 I-Iemiramphidae. Halfbeak Hyporliatrrpinis rrnf fasciatrrs Silverstripe Halfbeak 0 0 Synguathidae Pipefshes Syngnathua tscus Northern Pipefish 54 1 Syngnathus loidsianae Chain Pipefish 2b 89 Hippocarnpus ereetus Lined.Seahorse I 2 Duke Energy Progress, LLC A-11 Water Resources Unit Brunswick Steam Electric Plant 2022-2023 Biolo ical Monitorin Report Appendix 5.continued 2023 Total number Total weight(g) Scientific name Common name Trigliade. Searobins Prtonotus scittdus Leopard Searobin 2 10 Prfonows,tribulas Bighead.Searobin 21 269 Prionotus carolinus Northern Searobin 1 4 .Lutjanidae Snappers Lsr janiis griserrs Gray Snapper 1 2 Hemiramphus Halfbeaks Hendrarnphits spp. 1 19 Lepisosteidae Gars Lepisosteus osseus Longrose Gran 1 172 Gerreidae irfojarras TrishPom Pompano 1 6 Diaprerus aurarirs P Eucinostomus argentens Spotfin Mojarra 2 14 Haemulidae Grunts Orthopristis chrysoptera Pigfish 6 76 Pomatomidae Bluefishes Pomalomw saltatrix Bluefish 8 476 Carangidac Jacks Caranx hippos .Crevalle Jack 1 4 Selene vonrer Loakdown 14 25 8 Oligoplites saurus Leathaijacket fish 4 8 Trachinotus.carolinrts Florida Pompano 0 0 Chlorosc'ombrus chrysunts Atlantic.Bumper 2 4 Belonidne Needlefishes Strongyhrra marina Atlantic.Needlefish 0 0 Sparidne Porgies Archosargus probactocephalus Sheepshead 0 0 Lagodon rhombodies Pinfisb 26 362 Sciaenidae Drams 8 52 Baridiella clnysowu Silver Perch 41 2 Cynoscion nebulosars Spotted Seatrout 1 3 Cynoscion regalis Weakfish 1 42 Leiosioinus x 0 anthrinrs Spot 16 I,270 Menticirrhus.hiloralis Gulf Kingfish 0 Mieropogonias tindWrites Atlantic Croaker 104 .21543 Stellifer lanceolatus Star Drum 0 0 Menticirrhus anrericamrs Southern Kingfish 1 2 Epbippidae Spadefishes Chaetodiptenrsfaber Atlantic Spadefish 3 136 Mugilidae Mullets Mugu cepluilus Striped Mullet 8 632 Blennlidae Combtooth blennics Hypsoblennius hens 0 0 Feather Blenny 36 Hypsoblennius.ionthas Freckled Blenny 0 Chasniodes bosquianus Striped Blenny 1 2 Hyplenrodwitsgenrinatus. Crested Blenny 0 0 Trichiuridae Snake mackerels Trichiurus lepturia Atlantic'Cutlassftsh SS 1,444 Bothidae Lefteyeflounders Ancylopserta quadrocellata Ocellated flounder 2 23 Citharichthys spilopterus Bay Whiff I4 156 Etrppus crossotns. Fringed flounder 13 50 ParalichdO lethostigma Southern flounder 14 2,759 Duke Energy Progress, LLC A-12 Water Resources Unit Brunswick Steam Electric Plant 2022-2023 Biological Monitoring Report Appendix 5. continued 2023 Total number Totai weight(g) Scientific name Common name Cynoglossidae Toungefishes Syn1phurus plagiusa Blackcheek Toungefish 84 435 Balistidae Triggerfishes/Filesfishes Monacamhus hispidus, Planehead Filefish 1 19 Tetraodontidae Puffers Chdomyctena schoepfr. Striped Burrfish 22 2,272 Sphoeroides.uraculates IBorthem.Puffer 11 1,575 Lagocephalus laevigatus Smooth Puffer 0 0 Sphyraenidae Barracudas Sphyraena wrid. Barracuda sp. 0 0 Stromateidae Butterfishes es Harvestfish t6 313 Peprihis triacanthus Butterfish 3 1;4 Scophthalmidae Turbots Scophthaltnus aquosus Windowpane Flounder 0 0 Soleidne Soles Trinectes macuiuiirs Hogehoker 15 180 Invertebrates Laliginidae Pencil squids Lilliguncula brevis Atlantic Brier Squid 188 932 Squiltidne Mantis shrimps Squilla empasa Mantis Shrimp 18. 424 Penaeidne Prawns Farfantepenams astecus Browm shrimp 1,840 6,390 Farfantepenaeas duorartgn Pink Shrimp 158 399 Litopenaertsselifenrs White Shrimp 1,295 6,509 TrachypeneW constriclus Hardback Shrimp 119 94 Portudidae Swimming crabs Callinectes sapidirs Blue Crab 184 151392 Callineetesshnilis Usser Blue Crab 436 1,334 Ciillinectes spp. Swimming.crabs 1 I Porlunus spininianus Blotched Swimming Crab 2, 6 Porninus gibbeVi Iridescent Swimming Crab 16 128` 14timbers and biomass represent total number and weight collected per 24-hour sample summed over all sample dates. Duke Energy Progress,LLC A-13 Water Resources Unit