NC0090000_2024 Biological Intergrity Report_Colonial Pipeline Company_20240723

�i TRIC
July 19, 2024
Via Email Delivery
50 International Dr. T 864.281.0030
Suite 150 TRCcompanies.com
Greenville, SC 29615
QED
�1
North Carolina Department of Environmental Quality JUL 2 3 2�24
Division of Water Resources — Water Quality Permitting Section
NPDES 1617 Mail Center Service Center q��pDES
Raleigh, North Carolina 27699-1617 ����R� ► `r
Re: 2024 Annual Biological Integrity Monitoring Report
Colonial Pipeline Company — 2020-L1-SR2448 / Pipeline ROW Release
NPDES Permit No. NC0090000
Huntersville, Mecklenburg County, North Carolina 28078
To Whom it May Concern,
As required by National Pollution Discharge Elimination System (NPDES) Permit No. NC0090000, Section A.S.d
effective August 1, 2023, TRC Environmental Corporation (TRC) is submitting this Annual Biological Integrity
Report for the 2024 calendar year in connection with the Huntersville-Concord Road (State Road 2448 [SR 2448])
pipeline release located in Huntersville, Mecklenburg County, North Carolina on behalf of Colonial Pipeline
Company (Colonial). At the time of this submittal, the water treatment plant has not been constructed and no
discharge has occurred; therefore, this report will serve as documentation of baseline conditions. As required by
Section A.(5.)(b.), Colonial will conduct the next annual biological integrity sampling event prior to June 1, 2025
and submit the 2025 annual report within 60 days of the sampling event.
If you have any questions or concerns regarding the attached 2024 Annual Biological Integrity Monitoring
Report, please do not hesitate to contact Jeff Morrison with Colonial Pipeline at (770).819.3566.
Respectfully,
Chelsea Macionus
TRC

i.
AV r,
NPDES Biological Integrity Study Report
Predischarge Baseline Event
Colonial Pipeline Company (CPC)
NPDES Permit NCO090000
Huntersville, Mecklenburg County, North Carolina
Heather Smith
Senior Biological Scientist
Prepared by:
TRC Environmental Corporation
50 International Drive, Suite 150
Greenville, SC 29615
July 2024
K ren C. Saucier, Ph.D.
Vice -President, Senior Consultant
TRC Environmental Corporation I Colonial Pipeline Company
NPDES Biological Integrity Study Report
I (EMPLOYEES. ROOT. LOCAL I ENV( ECCI GREENVI LLEI WPGVL I PJT21568543100001 R5685430000-002 NPDES BI REPORT. DOCK
50 INTERNATIONAL DRIVE, SUITE 150, GREENVILLE, SC 29615, • 864.281.0030 PHONE . [phone] FAx • WWW.TR000MPANIES.COM

Table of Contents
Section1 Introduction.................................................................................................................................. 1
Section 2 In -Stream Assessment of Biological Integrity............................................................................... 3
2.1 Sampling Approach and Locations........................................................................................... 3
2.2 Sampling Methods...................................................................................................................3
2.3 Laboratory Methods................................................................................................................ 4
Section 3 Results and Discussion.................................................................................................................. 6
Section4 References..................................................................................................................................... 8
List of Tables
Table 1
Table 2
List of Figures
Figure 1
Figure 2
List of Appendices
Appendix A
WaterQuality Data..................................................................................................... 7
Macroinvertebrate Metrics......................................................................................... 7
SiteLocation Map....................................................................................................... 2
SampleLocation Map.................................................................................................. 5
MBI Report
TRC Environmental Corporation / Colonial Pipeline Company 1
NPDES Biological Integrity Study July 2024
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Section 1
Introduction
The Colonial Pipeline Company (CPC) was issued National Pollutant Discharge System (NPDES) Permit
NCO090000 associated with the 2020-L1-SR2448 Release Site located near Huntersville, Mecklenburg
County, North Carolina (the Site). NPDES Permit NCO090000 addresses a planned discharge from a
water treatment facility to be installed at the Site. CPC is required to perform in -stream biological
integrity assessments consistent with Permit Condition A. (5.) of the NPDES Permit. The Site is situated
on both sides of Huntersville-Concord Road (State Road 2448 [SR 2448]) as shown in Figure 1. CPC
contracted TRC Environmental Corporation (TRC) to develop the Biological Integrity Study Plan in
support of Permit Condition A. (5.) of NPDES Permit NC0090000. The Biological Integrity Study Plan was
submitted by CPC to North Carolina Department of Environmental Quality (NCDEQ) - Division of Water
Resources on October 31, 2023. This report documents the methods and results of the pre -discharge
baseline sampling event which occurred on May 20, 2024.
The area within a 1,500-foot radius of the Site is a mixture of low -density residential properties,
agricultural properties, and wooded land. The Site is located within the Yadkin Pee -Dee River Basin.
North Prong Clarke Creek is located approximately 1,800 feet north of the Site and South Prong Clarke
Creek is located approximately 2,700 feet south of the Site, both of which are characterized by the
NCDEQ Division of Water Quality as Class C water bodies, meaning that they are protected for non -
drinking water purposes such as biological integrity, fishing, and infrequent secondary recreational
purposes (i.e., wading).
Mecklenburg County is located within the Piedmont physiographic province, characterized by rolling
hills and moderately steep valleys formed by stream erosion of upland areas. Elevations at the Site
range from approximately 650 to 750 feet above mean sea level. The surficial soils at the Site consist
almost entirely of fine-grained clayey to silty saprolite developed from the weathering of the underlying
bedrock. The soil thickness ranges from a few feet to greater than 100 feet. Data obtained from
completed borings indicate the Site is underlain by quartz diorite.
TRC Environmental Corporation / Colonial Pipeline Company 1
NPDES Biological Integrity Study July 2024
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NORTH
8,ROLINA
�\5
I
"Wts:
Release Site —JET COLONIAL PIPELINE COMPANY
2020-LI-SR2448 RELEASE
HUNTERSVILLE, NC
SITE LOCATION MAP
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"El-3v R. SPRING
—101Eoe. B. FEUERSTEIN FIGURE I
— OCTOBER 2023
ROCK QUARRY ROAD
BASE MAP USGS TOPOGRAPHIC ONLINE SERVICE. 0 400 800 � �1429 PHONE 838SUITE 116
.C36 9668i
DATA SOURCES TRC, ESRI. USGS 0 FEET
27610
19.E00 P = 800'
WN ER8 NC

Section 2
In -Stream Assessment of Biological Integri
Permit Condition A. (5.) to the NPDES Permit NCO090000 requires an annual instream assessment of
biological integrity. The NCDEQ Full Scale method was used to determine bioclassifications and water
quality ratings. This method is typically used for most between -site and/or between -date comparisons
(NCDEQ 2016).
2.1 Sampling Approach and Locations
On May 20, 2024, TRC's biologist along with their subcontracted NCDEQ Certified Laboratory for Aquatic
Population Studies of Benthic Macroinvertebrates certified biologist conducted two Full -Scale benthic
macroinvertebrate surveys at two sampling locations —an Upstream Reference Site and a Downstream
Experimental Site. The sampling locations share similar riparian and geomorphological characteristics,
such as substrate composition and incised stream channel. The watersheds for the Upstream Reference
Site and the Downstream Experimental Site are comparable in that both watersheds are approximately
3 square miles in area and composed of forested lands, developed lands, agricultural lands, and
impervious surfaces (USGS StreamStatsl). The macroinvertebrate sampling locations for the pre-
discharge baseline sampling event are shown on Figure 2.
NCDEQ requires that North Carolina certified biologists implement the biological integrity assessment
program. TRC subcontracted a North Carolina certified biologist from Michael Baker International (MBI)
in Asheville, North Carolina to support preparation and implementation of the biological integrity
assessment program.
CPC notified the supervisor for the Biological Assessment Branch, Water Sciences Section 30-days prior
to performing the pre -discharge baseline sampling event.
2.2 Sampling Methods
TRC and MBI assessed in -stream and riparian habitat conditions at each of the sampling locations
consistent with the work plan developed for the Site. Metrics were used to generate a habitat score for
each sampling location and are included in Appendix A. Metrics include instream habitat, substrate
composition, pool variety, riffle habitat, bank stability and vegetation, light penetration, and riparian
vegetation zone width. Water quality parameters were collected at each sampling location with a
calibrated YSI style meter.
The macroinvertebrate samples were collected according to the specifications outlined in the 2016
NCDEQ Standard Operating Procedures for the Collection and Analysis of Benthic Macroinvertebrates,
TRC Environmental Corporation / Colonial Pipeline Company 3
NPDES Biological Integrity Study July 2024
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which provides detailed standard operating procedures for the collection and analysis of freshwater
benthic macroinvertebrate data that can help determine bioclassification ratings (NCDEQ 2016).
The techniques used to assess the macro invertebrate community included two riffle kicks, three sweeps,
one leaf -pack, two rock -and log -washes, one sand grab, and visual observations. To minimize the
amount of stirred -up sediment washing downstream and potential disturbance to macroinvertebrates,
sampling was initiated at the most downstream point of each reach with scientists traveling upstream to
the reference macroinvertebrate sampling location.
The riffle -kick method uses kicknets or D-frame nets, and substrate is surveyed for approximately two
meters. The kick -nets are placed perpendicular to the streambed and sediment is disturbed on the
upstream side of the kick -net so the disturbed contents wash into the net. The sweep collection method
requires a sweep net to be used to sample locations such as undercut banks, root mats and aquatic
vegetation. The leaf -pack collection method requires the collection of a leaf -pack, which should target
deciduous material when possible. The leaf -pack is collected and then the collected material is washed
and ultimately poured over a sieve bucket. The rock- and log -wash collection method requires larger
substrate material to be collected and then washed into a plastic container where the remaining
material is then poured through a fine -mesh sampler. The sand collection method specifically targets
macroinvertebrates that live in the sand. For the sand collection method, a kicknet was placed
downstream of the sand substrate to be sampled and sediment will be disturbed, and the disturbed
material will be collected in the kicknet. The visual method requires the sampling team make visual
inspections of the sampling locations mainly targeting larger macroinvertebrates (NCDEQ 2016). Any
larger macroinvertebrates were placed into a vial containing 70 percent ethanol. The remaining
contents from each Full -Scale sampling method was placed into a labeled container and preserved in 70
percent ethanol. The preserved samples were transferred to MBI's Asheville, NC based lab for
processing.
2.3 Laboratory Methods
After arrival at the lab, the contents of each sample were sorted to separate macroinvertebrates from
the organic material. Sorting was conducted under a dissecting microscope. Sorted macroinvertebrates
were preserved in 70 percent ethanol and identified to the lowest practical taxonomic level.
TRC Environmental Corporation / Colonial Pipeline Company 4
NPDES Biological Integrity Study July 2024
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NORTH
4R OLINA
Proposed
Outfall 001
Upstream
Reference Sample
wok c`'r�ek � ///tea•
Downstrearrf'Sample 1
SITE LOCATION
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21
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DATA SOIAtCES. TRC,ESRI,USGS
400 800
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1101ECT COLONIAL PIPELINE COMPANY
2020-L1-SR2448 RELEASE
HUNTERSVILLE, NC
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Section 3
Results and Discussion
Water quality data including Dissolved Oxygen (DO), pH, specific conductivity, and temperature were
collected at both locations using a calibrated YSI 556 MPS meter. Water quality parameters are
summarized in Table 1. Each water quality parameter was comparable at the upstream and
downstream locations.
Habitat within the Upstream Reference Site and Downstream Experimental Site was evaluated using the
Habitat Assessment Field Data Sheet for Mountain/Piedmont Streams. Appendix A contains the
assessment data sheets. The Upstream Reference Site is located within an area of heavy residential
development within the watershed. The reach sampled consists of a coarse sand substrate and deeply
incised banks. Large woody debris was present within the reach. Leaf packs, rocks, and bank habitat
were limited within the reach. The uniform habitat present in the upstream reach along with the sandy
substrate resulted in a Habitat Assessment Field Data Sheet for Mountain/Piedmont Streams total score
of 38. The Downstream Experimental Site contains coarse sandy substrate and provides undercut
habitat along the left bank along with a deeply incised right bank. One cobble riffle was observed in the
Downstream Experimental Site which resulted in a total habitat score of 49.
The macroinvertebrate data collected was used to calculate tolerance values and compared with the
North Carolina biotic index. Tolerance values are a macroinvertebrate index that refers to the pollution
tolerance levels of each macroinvertebrate taxon. The North Carolina Biotic Index (NCBI) is modeled
after the Hilsenhoff Biotic Index (Hilsenhoff 1987, 1988) and is a given sample's weighted average of
tolerance values for the macroinvertebrate species identified with respect to their abundance
(NCDEQ 2016). Table 2 summarizes benthic macroinvertebrate metrics calculated for each location.
The Downstream Experimental Site resulted in higher diversity and abundance when compared to the
upstream location. The Ephemeroptera, Plecoptera and Trichoptera (EPT) taxa richness score was higher
at the Downstream Experimental Site. These taxa are widely utilized as one measure of water quality
indicators because the richness and abundance of these organisms can be affected by a wide range of
environmental and water quality stressors. The Downstream Experimental Site scored a slightly lower
NCBI value, scaled from 0.0 to 10.0, where lower values indicate more pristine conditions and higher
values indicating stress. These differences may be attributable to the greater habitat diversity present
at the Downstream Experimental Site. Seasonal adjustment corrections are typically applied for select
species when sampling occurs outside of the summer sampling dates. The adjustment factors were not
applied to the Site data, as none of the taxa collected require application of the adjustment factor.
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Table 1
Water Quality Data
Site
Dissolved Oxygen
pH
Specific Conductivity (Nlcm)
Temperature (C°)
Upstream Reference
8.9
6.8
136
19.4
Downstream Experimental
10.8
7.3
138
18.4
Table 2
Macroinvertebrate Metrics
------
Site
------
Total
NCBI (Semi-
Taxa
EPT
Percent
Biological
Number of EPT
Rounding
Bioclassification
Number
Quantitative)
Richness
Richness
Intolerance
Integrity
Taxa Score
Decision
of
(Tolerance
Value
Individual
Value <_ 3)
Score
s
Upstream
194
6.53
19
6
1.61
2.4 14
2
Fair
Reference
Downstream
274
5.71
32
13
8.39
4
2
3
Good -Fair
Experimental
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Section 4
References
Hilsenhoff, W. L. 1987. Rapid field assessment of organic pollution with a family -level biotic index.
Journal of the North American Benthological Society, 7(1), 65-68.
Hilsenhoff, W. L. 1998. A modification of the biotic index of organic stream pollution to remedy
problems and permit its use throughout the year. The Great Lakes Entomologist, 31(1), 1.
North Carolina Department of Environmental Quality, Division of Water Resources, Water Sciences
Section, Biological Assessment Branch, 2016. Standard Operating Procedures for the Collection
and Analysis of Benthic Macroinvertebrates (Version 5).
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NPDES Biological Integrity Study July 2024
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Appendix A
MBI Report
TRC Environmental Corporation / Colonial Pipeline Company
NPDES Biological Integrity Study July 2024
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INTERNATIONAL
July 5, 2024
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Colonial Pipeline Company
NPDES Permit NCO090000
2024 Benthic Macroinvertebrate Monitoring Report
Annual Instream Assessment of Biological Integrity Study
Introduction:
The Colonial Pipeline Company (CPC) Huntersville-Concord Road (State Road 2448 [SR 2448]) pipeline site is located
near Huntersville, Mecklenburg County, North Carolina (the Site). The CPC pipelines cross SR 2448 (Figure 1).
The area within a 1,500-foot radius of the Site is a mixture of low -density residential properties, agricultural
properties, and wooded land. The Site is located within the Yadkin Pee -Dee River Basin. North Prong Clarke Creek is
located approximately 1,800 feet north of the Site and South Prong Clarke Creek is located approximately 2,700 feet
south of the Site, both of which are characterized by the NCDEQ Division of Water Quality as Class C water bodies,
meaning that they are protected for non -drinking water purposes such as biological integrity, fishing, and infrequent
secondary recreational purposes (i.e., wading).
Mecklenburg County is located within the Piedmont physiographic province, characterized by rolling hills and
moderately steep valleys formed by stream erosion of upland areas. Elevations at the Site range from approximately
650 to 750 feet above mean sea level. The surficial soils at the Site consist almost entirely of fine-grained clayey to
silty saprolite developed from the weathering of the underlying bedrock. The soil thickness ranges from a few feet to
greater than 100 feet. Data obtained from completed borings indicate the Site is underlain by quartz diorite.
Requirements:
TRC Companies (TRC) enlisted Michael Baker International (MBI) to design an Annual Instream Assessment of
Biological Integrity Study Plan in the North Prong of Clark Creek in Mecklenburg County, North Carolina. MBI operates
a NC Division of Environmental Quality (DEQ) Certified Laboratory for Aquatic Population Studies of Benthic
Macroinvertebrates (Certificate #044). The study plan and annual survey are required for compliance with the terms
of Colonial Pipeline Company's NPDES Permit NCO090000 Section A. (5.) Instream Assessment of Biological Integrity
(Annual). This NPDES Permit is associated with a proposed discharge (Outfall 001) into the North Prong of Clark Creek
where the creek crosses under Huntersville — Concord Rd. at approximately 35.420216,-80.797979.
Methods:
On May 20, 2024, MBI staff Jason York and Brent Stoltz, along with TRC employee James Treacy
conducted two Full -Scale benthic macroinvertebrate samples on the North Fork of Clark Creek in
accordance with the approved Annual Instream Assessment of Biological Integrity Study Plan. The Full -
Scale sampling method includes':
• Two riffle -kicks
• Three sweeps
1 NC Department of Environmental Quality. 2016. Standard Operating Procedures for the Collection and Analysis of Benthic
Macroinvertebrates. Division of Water Resources. Raleigh, North Carolina. February 2016.

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I N T E R N AT 1 0 N A L
• One leaf -pack
• Two rock and log washes
• One sand
• Visual
Water quality data including Dissolved Oxygen (DO), pH, Specific conductivity, and temperature were
collected at both locations using a calibrated YSI 556 MPS meter from Field Environmental Instruments,
Inc. and is shown in Table 1 of the Discussion section of this report. A taxa list for each site is included
in Appendix A.
Site Descriptions:
The Upstream Reference Site is located at approximately 35.419315,-80.806036 and was accessed
through private property at the end of Ewart Rd. Heavy residential development exists in the
watershed upstream. The sampled reach is deeply incised with the substrate consisting almost entirely
of course sand. Large woody debris was frequent; however, leaf packs, rocks, and bank habitat were
limited. Due to a severe lack of colonizable habitat and an almost completely uniform sand substrate,
the Habitat Assessment Field Data Sheet for Mountain/Piedmont Streams Total Score was 38.
Reference location facing upstream.
Reference location facing downstream.

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INTERNATIONAL
The Downstream Experimental Site is located at approximately 35.419590,-80.797398 and was
accessed through private property immediately past the Huntersville-Concord Rd bridged crossing of
the North Prong of Clark Creek. The sampled reach was deeply incised on the right bank. The left bank
did provide some undercut habitat; however, woody debris and leaf packs were limited. One cobble
riffle was present although the substrate was dominated by coarse sand. Due to the presence of this
riffle the Total Score for the Habitat Assessment Field Data Sheet for Mountain Piedmont Streams at
this site was 49.
Experimental site facing downstream.
Discussion:
rf„ a
r tr
5 _
A.
Experimental site facing upstream.
Physicochemical water quality parameters between the Upstream Reference site and the Downstream Experimental
site showed minimal difference (See Table 1); however, benthic macroinvertebrate metrics at the Downstream
Experimental site showed higher diversity and abundance which is summarized in Table 2 and shown visually in Figure
2. The Downstream Experimental site also scored a lower NC Biotic Index value, scaled from 0.0 to 10.0, where lower
values indicate more pristine conditions and higher values indicating stress. These differences can be most likely
explained by greater available habitat at the Downstream Experimental location which included a rocky riffle and
undercut banks that were not available at the Upstream Reference location. Bioclassifications were assigned to each
site based on "Table 5. Thresholds for determining BI and EPT scores using Full Scale criteria" from the 2016 NC DEQ
Standard Operating Procedures for the Collection and Analysis of Benthic Macroinvertebrates (2016 SOP). Seasonal

NORTH
4ROLINA
Outfall 001
Upstream Reference
Site
i
Downstream
Experimental Site
- - - - - SITE LOCATION
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SITE LOCATION MAP
DRAWN By N. BILLINGS RRa. No. 568543
CHECLFD BY R. SPRING
APAwmBr B. FEUERSTEIN FIGURE 1
DATE OCTOBER 2023
BASE MAP USGS TOPOGRAPHIC ONLINE SERVICE. 0 400 [^ 1429 ROCK QUARRY ROAD
SUITEDATA SOURCES. TRC, ESRI, USGS � FEET T� ` PHONE. 3, C 8881
19,600 1'
FLE .NTERSWLL NC

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I N T E R N AT 1 0 N A L
corrections did not need to be applied to the data despite the collection date of May 20th, which falls outside of the
summer sampling season of June 1st — September 30th, as only one Plecoptera taxa (Perlesto spp.) was collected
which is not included on the list of taxa used in seasonal adjustments (Table 4. 2016 SOP).
TABLE 1: WATER QUALITY DATA
SPECIFIC
SITE
DISSOLVED
pH
CONDUCTIVITY
TEMPERATURE
OXYGEN
(µ/cm)
(Cl
UPSTREAM
8.9
6.8
136
19.4
REFERENCE
DOWNSTREAM
10.8
7.25
138
18.4
EXPERIMENTAL
TABLE 2: MACROINVERTEBRATE
METRICS
NCBI (Semi-
Taxa
EPT
%Into)
BI VALUE
# OF EPT TAXA
ROUNDING
SITE
Total #I
BIOCLASSIFICATION
Quantitative)
Richness
Richness
(TV <_ 3)
SCORE
SCORE
DECISION
UPSTREAM
194
6.53
19
6
1.61
2.4
1.4
2
FAIR
REFERENCE
DOWNSTREAM
274
5.71
32
13
8.39
4
2
3
GOOD -FAIR
EXPERIMENTAL
Figure 2: Comparison of Site Metrics
NCBI (Semi-Quantitative)ES
5.71
6.53
13
EPT Richness OM
Enr6
Taxa Richness
19
0 5 10 15 20 25 3(�
■ DOWNSTREAM EXPERIMENTAL 0 UPSTREAM REFERENCE

I N T E R N AT 1 0 N A L
APPENDIX A
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TAXA LIST AND HABITAT FORMS

North Prong Clark Creek Upstream Reference Location
FINALID
ORDER
FAMILY
GENUS
SPECIES
TOLERANCE
COUNT
ACR
notes
Baetis intercalaris
Ephemeroptera
Baetidae
Baetis
intercalaris
5
11
A
Plauditus cestus
Ephemeroptera
Baetidae
Plauditus
cestus
4.6
1
R
Maccaffertium modestum
Ephemeroptera
Heptageniidae
Maccaffertium
modestum
5.7
2
R
Porocloeodes spp
Ephemeroptera
Baetidae
Paracloeodes
spp
8
1
R
Hydropsyche (C.) sporna
Trichoptera
Hydropsychidae
Hydropsyche
(C.) sporna
2.5
3
C
Cheumotopsyche spp
Trichoptera
Hydropsychidae
Cheumotopsyche
spp
6.61
28
A
Ischnurospp
Odonata
Coenagrionidae
Ischnura
spp
9.5
1
R
Calopteryx spp
Odonata
Calopterygidae
Colopteryx
spp
7.5
2
R
Progomphus spp
Odonata
Gomphidae
Progomphus
spp
8.2
2
R
obscurus
Boyeria vinosa
Odonata
Aeshnidae
Boyeria
vinosa
5.8
1
R
Rhogovelio t
Hemiptera
Vehidae
Rhogovelio
spp
8
C
Tipulo spp
Diptera
Tipulidae
Tipula
spP
7.5
13
A
Simulium spp
Diptera
Simuliidae
Simulium
spp
4.91
52
A
Chironomusspp
Diptera
Chironomidae
Chironomus
spp
9.3
3
C
Brillio spp
Diptera
Chironomidae
Brillio
spp
5.7
6
C
Cryptochironomus spp
Diptera
Chironomidae
Cryptochironomus
spp
6.4
6
C
Polypedilum illinoense gr
Diptera
Chironomidae
Polypedilum
illinoense gr
8.7
32
A
Physo spp
IGastropoda
Physidae
jPhysa
Ism
8.7
2
R
Corbiculofluminea
jBivalvia
ICorbiculidae
lCorbicula
Ifluminea
6.6
20
A
North Prong Clark Creek Downstream Experimental Location
FINALID
ORDER
FAMILY
GENUS
SPECIES
TOLERANCE
COUNT
ACR
notes
Baetis intercalaris
Ephemeroptera
Baetidae
Baetis
intercalaris
5
15
A
Baetis pluto
Ephemeroptera
Baetidae
Baetis
Pluto
3.4
1
R
Baetis flovistrigo
Ephemeroptera
Baetidae
Boetis
flavistriga
6.8
27
A
Maccaffertium modestum
Ephemeroptera
Heptageniidae
Maccaffertium
modestum
5.7
8
C
Maccaffertium terminotum
Ephemeroptera
Heptageniidae
Maccaffertium
terminotum
4.4
1
R
Maccaffertium meririvulanum
Ephemeroptera
Heptageniidae
Maccaffertium
merifivulanun
0.5
1
R
Plauditus dubius gr
Ephemeroptera
Baetidae
Plouditus
dubius gr
2.2
9
C
Lobioboetis propinquus
Ephemeroptera
Baetidae
Lobioboetis
propinquus
5.8
1
R
Perlesto spp
Plecoptera
Perlidae
Perlesto
spp
2.9
1
R
Hydropsyche (H.) betteni
Trichoptera
Hydropsychidae
Hydropsyche
(H.) betteni
7.9
1
R
Hydropsyche (C.) sporna
Trichoptera
Hydropsychidae
Hydropsyche
(C.) sporna
2.5
10
A
Cheumotopsyche spp
Trichoptera
Hydropsychidae
Cheumotopsyche
spp
6.61
67
A
Chimorrospp
Trichoptera
Philopotamidae
Chimorra
spp
3.3
1
R
Progomphusspp
Odonata
Gomphidae
Progomphus
spp
8.2
1
R
Boyeria vinosa
Odonata
Aeshnidae
Boyeria
vinosa
5.8
4
C
Ophiogomphus spp
Odonata
Gomphidae
Ophiogomphus
spp
5.9
1
R
Argio spp
Odonata
Coenagrionidae
Argia
spp
8.3
2
R
Stenelmis spp
Coleoptera
Elmidae
Stenelmis
spp
5.6
7
C
Corydolus cornutus
Megaloptera
Corydalidae
Corydalus
cornutus
5.2
1
R
Tipula spp
Diptera
Tipulidae
Tipula
spp
7.S
7
C
Antocho spp
Diptera
Tipulidae
Antocho
spp
4.4
2
R
Simulium spp
Diptera
Simuliidae
Simulium
spp
4.9
48
A
Polypedilum illinoense gr
Diptera
Chironomidae
Polypedilum
illinoense gr
8.7
18
A
Cryptochironomus spp
Diptera
Chironomidae
Cryptochironomus
spp
6.4
1
R
Porometriocnemusspp
Diptera
Chironomidae
Parometriocnemus
spp
3.9
3
C
Apedilum t
Diptera
Chironomidae
Apedilum
spp
5.69
3
C
JTN Tolerance Value
Micropsectra spp
Diptera
Chironomidae
Micropsectro
spp
2.4
2
R
Phoenopsectro punctipes gr
Diptera
Chironomidae
Phoenopsectra
punctipes gr
7.1
5
C
Eukiefferiello claripennis gr
Diptera
Chironomidae
Eukiefferiella
cloripennis gr
6.2
2
R
Cambarusspp
ICrustacea
Cambaridae
ICombarus
spp
7.5
1
R
Sphaerium spp
jBivalvia
Sphaeriidae
ISphoerium
Ispp
7.2
3
C
Corbicula fluminea
jBivalvia
Corbiculidae
lCorbicula
Vluminea
1 &61
201
A

N Cl�C
Appendix A Mountain/Piedmont Habitat Assessment Form
I 1 /l3 Revision 8
Habitat Assessment Field Data Sheet
Mountain/ Piedmont Streams
Biological Asses ent Braeck DW R tTOTA L SCn_ R E
Llrimcrions for use: The observer is to survey a minimum of 100 meters with 200 meters preferred of stream, preferably in an upstream direction starting
above the bridge pool and the mad right-of-way. The segment which is assessed should represent average stream conditions. To perform a proper habitat
evaluation the ohserva.. needs to get into the stream. To complete the form, select the description which best fits the observed habitats and then circle the score.
If the observed habitat falls in between two descriptions, select an intermediate score. A final habitat score is determined by adding the results from the different
mcmcs.
Stzeam � �%►� ItLocation/road: (Road Name )County Mic'L
Date 2-0 —CClt __Basin_Vri�tl.cr it I'U —Olk tubbasin
Obsmvcr(s) Type of Study: ❑ Fish O e"thos ❑ Basinwide ❑Special Study (Describe)
Laruxie Longitude Ecoregion: ❑ MT Vf ❑ Slate Belt ❑ Triassic Basin
Water Quality: Temperature ! jt'�-C DO f!l U mg/I Conductivity (corr.) f " _-AS/cm pH � J
Physical Characterization: Visible land use refers to immediate area that you can see from sampling location - include what you estimate driving thru
the watershed in watershed land use.
p�
Visible Land Use: _ / U %Forest %Residential %Active Pasture % Active Crops %Fallow Fields
Commercial %indur+tnal _ %Other - Describe:
Watershed land use : ❑Forest ❑Agriculture Durban ❑ Animal operations upstream
Width: (meters) Stream____ Channel (at top of bank) �K Stream Depth: (m) Avg_. J Max _
❑ Width variable ❑ Large river>25m wide
Bank Height (from deepest part of riffle to Lop of bank -first flat surface you stand on): (m) L S
Bank Angle: _T—" or ❑ NA ( Vertical is 90°, horiumtal is 0°. Angles > 90" indicate slope is towards mud -channel, < 90' indicate slope is away from
channel. NA if bank is too low for bank angle to matter.)
❑ Channeliz-ed Ditch
❑Deeply incised -steep, straight banks 013oth banks undercut at bend ❑Channel tilled ui with sediment
❑ Recent overbank deposits ❑Bar development ❑liwied structures ❑Exposed bedrock
37

Appendix A - Mountairt/Pledmont Habitat Assessment Form
❑ Excessive penphyton gmwth ❑ Heavy filamentous algae growth OGreert tinge D Sewage smell
Mt de Stabnl,zation: Y: ❑Ri"p, cement, gabions O Sediment/grade-control structure OBerm/levee
Flow -cc ditin . vrtnal ❑t,ow
1 OCkw i�Kjg;ly Turbid 011abid ❑Tannic ❑Mil . Colored (from dyes)
Good poftntial for Wedan& Restoration Project?? D YES ONO Details
C_bfinnel F %— Status
Useful esrt~eij4- under abnow al or low flow conditions.
A. Watex reaches base of both lower bank&. minimal channel substrate exposed ............................ O /
B. Wawr fills >75% of available channel, or Q5% of channel substrate is exposed ........................
C. Water fills 25-75`iG of available channel, many logs/snags exposed ............................................. D
D. Root meets cxfi of watcr....................... ........ -................................................................... ............... O
E. Very little weer in chanrm), mostly present as standing pools/OD.gital
...........................................Weatber Con�ditians: Photos: ❑N 035mm
L Cbannel Modifieatioo Score
A. chaimel nantral frequent bends........................................................................................................ 5
B. channel nawral, infrequent bends (channelization could be old) ......................................................
C. some chaanchzeLon present..............................................................................................................
D. more extensive char Iirrtion, >401/6 of stream disrupted...............................................................
E. no bends, completely channelized or rip rapped or gabioned, etc ..................................................... 0
❑ Evidence of dredging ❑Evidence of debTAWo tg=no large woody debris in stream D Banks of uniform shape/height
Remarks Subtotal
H. tDatream dabitat: (txuider the pem4litaW of the reach that is favorable for benthos colonization or fish cover. If >70*9 of the rvdch is rocks. 1 tyre is
p[Cterci, curie the score of 17. Definition:: ltafpacks consist of older leaves that are packed together and hasr l+rbun to decay (not piles of leaves in pool arras).
Mark as Rare. Common. or Abundant
k"Rocks 1� Macrophytes=-StickA and Itrfprclu _,(-Snags rood lugs &I.Indercul banks or rout rows
38

Appendix A — Mountain/Piedmont Habitat Assessment Form
AMOUNT OF REACH FAVORABLE FOR COLONIZATION OR COVER
4 or 5 types present .................
3 types present ........................
2 types present ........................
1 type present ..........................
No types present .....................
❑ No woody vegetation in riparian aone Remarks_
>70% 40-70% 20-40% <20%
Score Score Score
20 16 8
19 15 11 7
18 14 10 6
17 13 9 5
0
Subtotal
III. Bottom Substrate (silt, sand, detritus, gravel, cobble, boulder) Look at entire reach for substrate scoring, but only look at riffle for embeddedness. and
is rocksSfrom-3i 45ok for "mud line" or difficulty extracting rocks.
A. substrate with good mlz of gravel, cobble and boulders
Score
1. embeddedness <20% (very little sand, usually only behind large boulders) .........................
15
2. embeddedness 20401/6 ..........................................................................................................
12
3. embeddedness 40-80%..........................................................................................................
8
4. embeddedness>80%.............................................................................................................
3
B. substrate gravel and cobble
1. embeddedness<20%............................................................................................................
14
2. embeddedness 20-40%.........................................................................................................
11
3. embeddedness 40-800/a ........................................................................................................
6
4. embeddedness>80%............................................................................................................
2
C. substrate mostly gravel
1. embeddedness<500/c ............................................................................................................
2. embeddedness>500/a ............................................................................................................
D. substrate homogeneous
1. substrate nearly all bedrock...................................................................................................
2. substrate nearly all sand........................................................................................................
3. substrate nearly all detritus....................................................................................................
4. substrate nearly all silt/ clay...................................................................................................
Remarks
4
3
2
1
Subtotal_ _
IV. Pool Variety Pools are areas of deeper than average maximum depths with little or no surface turbulence. Water velocities associated with pools are
always slow. Pools may take the form of"pocket water', small pools behind boulders or obstructions, in large high gradient streams. or side eddies.
A. Pools present sm
1. Pools Frequent (>300/c of 200m area surveyed)
a. variety of pool sizes............................................................................................................... I U
b. pools about the same size (indicates pools filling in)........................................................... K
39

Appendix A — Mountain/Piedmont Habitat Assessment Form
2. Pools Infrequent (Q0% of the 200m area surveyed)
a. variety of pool sizes. .......... ......................................................................................... ... (r
b. pools about the same size..................................................................................................
B. Pools absent_........................................................................
Subtotal
Pool bottom boulder-eobble,=hard. ttom sandy -sink as you walk ❑ Silt bottom ❑ Some pools over wader depth
marks
V. Riffle Habitats
Definition: Riffle is area of reaetation-can be debris dam, or narrow channel area. Riffles Frequent
'core
A. well defined riffle and rum, riffle as wide as stream and extends 2X width of stream.... 16
B. riffle as wide as stream but riffle length is not 2X stream width .................................... 14
C. riffle not as wide as stream and riffle length is not 2X stream width ............................. 10
D. riffles absent.. ................................................................................................................ 0
Channel Slope: OTypical for area OSteep—fast flow ❑Low=like a coastal stream
VI. Bank Stability and Vegetation
A. Erosion
1. No, or very little, erosion present ...............
2. Erosion mostly at outside of meanders .................................
3. Less than 50% of banks eroding
4. Massive erosion...................................................................... 0 Erosion _
B. Bank Vegetation
1. Mostly mature trees (>12" DBH) present .............................. 7
2. Mostly small trees (<12" DBH) present, large trees rare .....�)
3. No trees on bank, can have some shrubs and grasses............''
4. Mostly grasses or mosses on bank ......................................... 2
5. Little or no bank vegetation, bare soil everywhere ................ 0 Vegetation Score___
Remarks
Page Total.
Riffles Infrequent
Score
�7/
3
Subtotal
Subtotal
VII. Light Penetration Canopy is defined as tree or vegetative cover directly above the stream's surface. Canopy would block out sunlight when the sun is
directly overhead. Note shading from mountains, but not use to score this metric.
A. Stream with good canopy with some breaks for light penetration .............................................
B. Stream with full canopy - breaks for light penetration absent ................................................... 8
C. Stream with partial canopy - sunlight and shading are essentially equal .................................... 7
D. Stream with minimal canopy - full sun in all but a few areas.. ...... .... ......................................... 0
E. No canopy and no shading ... ...... ......................................................................... I...................
40

Remarks
Appendix A — Mountain/Piedmont Habitat Assessment Form
Subtotal
Vlll. Riparian Vegetative Zone Width
Definition: Riparian zone for this form is area ofnatural vegetation adjacent to stream (can go beyond floodplain). Definition: A break in the riparian zone is any
place on the stream banks which allo\\N sediment or pollutants to directly enter the stream, such as paths down to stream, storm drains, uprooted trees, otter
slides, etc.
/ FA UPST AM
Dominant vegetation: a4rem S rubs ces eeds/old field ❑Exotics (kudzu, etc)
A. Riparian zone intact (no breaks)
1. width > 18 meters .....................................................................................
2. width 12-18 meters ....................
3. width 6-12 meters.....................................................................................
4. width < 6 meters......................................................................................
B. Riparian zone not intact (breaks)
1. breaks rare
a. width > 18 meters.........................................................................
b. width 12-18 meters.......................................................................
c. width 6-12 meters .......................................................................
d. width < 6 meters.........................................................................
2. breaks common
a. width > 18 meters .........................................................................
b. width 12-18 meters......................................................................
c. width 6-12 meters.......................................................................
d. width < 6 meters.........................................................................
Remarks
O Disclaimer -form filled out, but score doesn't match subjective opinion -atypical stream.
Lft. Bank Rt. Bank
Score Score
5
& 3
2 2
3 3
2 2
1 1
0 0
Subtotal
Page Total
TOTAL SCORE
41

of5i�`�°ttiw'(
Appondf,w A _ Mountain/PledmOnt Habitat AswSS%rrent Fbrm
11113 Revision 3
Habitat Assessment Field Data Sheet
Mountain/ Piedmont Streams
Biolttgieal 4_xsessment Branch, DNVR 'TOTAL SCORE
.., _ _. R.v observer is io survey a minimum of 100 meters with 200 meters preferred or ,tree.,., preierabiy in an. up3tream direction starting
f hndge pool, and the mad right-of-v%ay, i he segment whtich is assessed should represent average stream conditions, T(, perform a proper[aabiltat
evaluation the observe nc:a s w. g-,t into the stream. To complete the form, select the description which best fits the observed habirats ,and then c-ircie the :x:urc.
ifs close: w habitat ill: ir, c, oh-cen two descriptions, select an intermediate score. A Final habitat score 'is ol&ermined by adding the results tirom the diiFferent
Mules.
saw . I • /1G1:-ClkleL (_I C 6ion/road (Road T'a!; e 1Cuu.^,'.y
r --L Fe ��
I>t:31e_ i � � CC1t Basin �. GZ _ �. Subbasin_
O mcrtst Type of Study: ❑ Fish ent)tos ] Basinwide 013' pal Study (Describe)
1`�t iit.�rla nnmt. vin
F.cores:on: 17 MT ❑Slate f3ctt ❑Triassic E3asln
Wimter Qualtt- TernDcratllfC 0. 7°C % mg/i Conductivity (corn.) j ILS/cm PH
Phvsli al Ciliaraeterimition- Visible and use refers t,� irmpolnte aren that vetme can see from sampling lucatiun - includt whin you 17timate (ruing thr11
`
land use.
Visible Land Use: V" %Forest %Residential
%Active Pasture `% Crops %Fallow- Fields (%
Cr rame: =_.' %�a! °/*O'Lher - D cribe.
—
Watershed )snd use: 13FOtest DAgriculture than 0 Animal operations upstream
Width- (meters) Stream_ Channel (at top of bank)_ Stream Depth: (m) Avg, I � (41a; .ice S
(13 Width v;uiable D Large river>25m wide
)plank Height (from deepest pan of .riffle to top of bank -first Flat surface
you stand on): (m)_
cj
I
Bank Angle " or ❑ NA (Vertical is 9(1v, Iwriiuntal is tl°.
channel. NA if .s too low for bank ungle to matter.)
Angles > 4(° indicate slope is towards mid -channel, < 90' Indicate slope is away prom
P+1nr�nelized Ditch
lumko undercut at bend
6u r;Iy incised -steep, straibh! banks 0Kiarildevelopmeni
Q Rcceni
C u!!ttiel i)i(cd in with sedim alt
oycrbarlk C,teposlls td
nn^••ri4•! ii 1^ w!t,rch DExp sud hedrt.vk
37

Appendix A Mountain/Piedmont Habitat Assessment Form
❑ Excessive penphyton gma-th ❑ Heavy filamentous algae growth ❑Green tinge ❑ Sewage smell
Manmade Stabilization: ❑pt Y: ❑Rip -rap, cement, gahions ❑ Sedhncnt/grade-control atntcture ❑Ilerrn/levee
Flo,A conditions ❑H�r� ormal ❑Lou
Turb[]ClearGP iditN Clear 'tilighth Turbid ❑Turbid ❑Tannic ❑Mil ❑Colored (from dyes)
Good potential for %N etlands Restoration Project?? ❑ \ ES 0 Detalli
Channel Flo- Status
Useful espectalh under abnormal or lon flow conditions.
A. Water reaches base of both lower banks, minimal channel substrate exposed ............................ El
B. Water fills >75% of available channel, or -25% of channel substrate is exposed ........................
C. Water fills 25-75% of available channel, many logs/snags exposed ............................................. ❑
D. Root mats out of water................................................................................................................... ❑
E. VerN little water in channel, mostly present as standing pools ..................................................... ❑ /
Weather Conditions: Photos: ON @Y ❑ Digital 035mm
Remarks: J-1Z�f [L.t1�7t'wflc� --
1. Channel Modification Scott
A. channel natural, frequent bends........................................................................................................ 5
B. channel natural, infrequent bends (channelization could be old) ......................................................
C. some channelization present..............................................................................................................
D. more extensive channelization, >40% of stream disrupted............................................................... 2
E. no bends, completely channelized or rip rapped or gabioned, etc ..................................................... 0
❑ Evidence of dredging ❑Evidence of desnaWing=no large woody debris in stream ❑Banks of uniform shape/height Remarks Subtotal-4
11. Instream Habitat: Consider the percentage of the reach that is favorable for benthos colonization or fish cover. If >700/6 of the reach is rucks, I type is
present, circle the score of 17. Definition: leafpacks consist of older leaves that are packed together and have begun to decay (not piles of leaves in pool areas).
Mark as Rare. Common, or Abundant. 1
Rocks gMacrophytes -,/—Sticka and leafpacka _Snags and logs Undercut banks or root mats
L L
38
ELI

Appendix A — Mountain/Piedmont Habitat Assessment Form
AMOUNT OF REACH FAVORABLE FOR COLONIZATION OR COVER
4 or 5 types present .................
3 types present .........................
2 types present .........................
14, 1 type present ...........................
No types present .......................
❑ No Moody uegautio in npanan zone
>70%
40-70%
20-40%
<20%
Score
Score
20
16
2
8
19
15
1
7
18
14
10
6
17
13
9
5
0
Subtotal
Ill. Bottom Substrate (slit, sand, detritus, gravel, cobble, boulder) Look at entire reach for substrate scoring, but only look at riffle for embeddedness, and
use rocks from all pans of riffle -look for "mud line" or difficulty extracting rocks.
A. substrate with good mix of gravel, cobble and boulders
Score
1. embeddedness LO%(very little sand, usually only behind large boulders) .........................
15
2. embeddedness 20-40%..........................................................................................................
12
3_ embeddedness 40-80%..........................................................................................................
8
4. embeddedness>80%.............................................................................................................
3
B. substrate gravel and cobble
1. embeddedness<20%............................................................................................................
14
2. embeddedness 20-400/6 .........................................................................................................
11
3. embeddedness 40-80%........................................................................................................
6
4. embeddedness>80%............................................................................................................
2
C. substrate mostly gravel
1. embeddedness<50%............................................................................................................
8
2. embeddedness>50%............................................................................................................
4
D. substrate homogeneous
1. substrate nearly all bedrock...................................................................................................
2. substrate nearly all sand........................................................................................................ (3 )
3. substrate nearly all detritus....................................................................................................
4. substrate nearly all silt/ clay................................................................................................... 1
Remarks Subtotal
IV. Pool Variety Pools are areas of deeper than average maximum depths with little or no surface turbulence. Water velocities associated with pools are
always slow. Pools may take the form of"pocket water", small pools behind boulders or obstructions, in large high grudient streams, or side eddies.
A. Pools present Sc ire
1. Pools Frequent (>300/a of 200m area surveyed)
a. variety of pool sizes............................................................................................................... l0
b. pools about the same size (indicates pools filling in)............................................................ 8
39

Appendix A — Mountain/Piedmont Habitat Assessment Form
2. Pools Infrequent (©00/6 of the 200m atra surveyed)
a. variety of pool sires...............................................................................................................
b. pools about the same size................................................................. ...............................
B. Pools absent... ................................. ,.................................................................................................. 4
Subtotal
❑ Pool bottom houlder-cobble=hard ottom sandy -sink as you walk O Silt bottom O Some pools over wader depth
Remarks
V. Riffle Habitats
Definition: Riffle is area of maeration-can be debris dam, or narrow channel area. Riffles Frequent
Score
A. well defined riffle and run, riffle as wide as stream and extends 2X width of stream.... 16
B. riffle as wide as stream but riffle length is not 2X stream width .................................... 14
C. riffle not as Wide as stream and riffle length is not 2X stream width ............................. 10
D. riffles absent.............................................................................................. 0
..........
Channel Slope: ❑Typical for area OSteep--fast flow ow=like a coastal stream
V1. Bank Stability and Vegetation
A. Erosion
1. No, or very little, erosion present ........................................... 7
2. Erosion mostly at outside of meanders ..................................&)
3. Less than 50% of banks eroding ............................................
4. Massive erosion...................................................................... 0 Erosion Score
B. Bank Vegetation
1. Mostly mature trees (> 12" DBH) present .............................. 7�
2. Mostly small trees (<12" DBH) present, large trees rare ...... 5
3. No trees on bank, can have some shrubs and grasses............ 3
4. Mostly grasses or mosses on bank ......................................... 2
5. Little or no bank vegetation, bare soil everywhere ................ 0 Vegetation Score
Remarks
Page Toed,_,_,-_
Riffles Infrequent
Score
12
b
Subtotal ' a
VII. Light Penetration Canopy is defined as tree or vegetative cover directly above the stream's surface. Canopy would block out surdigbt
directly overhead. Note shading from mountains, but not use to score this metric.
A. Stream with good canopy with some breaks for light penetration .............................................
B. Stream with full canopy - breaks for light penetration absent .................................. I..................
C. Stream with partial canopy - sunlight and shading are essentially equal ....................................
D. Stream with minimal canopy - full sun in all but a few areas .......................................................
E. No canopy and no shading............................................................................................................

Appendix A — Mountaln/Pledmont Habitat Assessment Form
Renutrks Subtotal
VUt. Riparian Vegetative Zone Width
Definition-0 sQn-,- `<� for this rr�-6c area ofnntutnl vcgctntitm adjacent to etrenm jean go beyond ttaudpla9n). Definition: A break in tfie ripairian :?bnc is any
tab= lu^: sttaem b.•.rU'Ne'll a11: u7 Aliment or polhnenas to directly enter ►.tic Streum. ouch as patlia r:1o'wn rto !stream, ,rm drains, uprooted Trees, :vier
stick, etr f
! FACE UPSTR! AM Ln. Bank Rt. Bank
Dominant inetation: Vlh-x. 04tvhs El Gras=& u VVeedvold field 1]Lxotics tkudru, etc) Score Score
intact (no !sneaks)
1. M,odti� *- 1ftMereiS............ti...... ........................... ........ I ........ I..................... (�
') wiAt1. 1 1 G ........ I 4
3. Wift, l..`i = rTYcici :...........».................................................................... 3
4. -Width i tt meicl1...................................................................................... 2 2
B. Riparim; Tor- no! !atact (breaks)
1. b^tals r°rr
AL %idt}►> i8 metem......................................................................... .4 •3
b. width 12- 1a mctcrs....................................................................... 3 3
C. W-Ji 'i 6-12 Mete;........................................................................ 2 2
L. wi= < Ll .it= .................. ....................................................... I 1
btts� CoflUzW
a. width > 18 meters......................................................................... 3 3
(`. iiw.ti: 1i-i- w""'wie...». ................................................................ 2
c. width 6-12 tt-1 te......................................................................... 1 1
Rcrn iri�c d. width-6-neiem......................................................................... 0 o 01
Subtotal
Page Total___
[7 Disclaimer -form filled out- bin score doesn't match subjective ovipion-atYPteet atroam• TOTAL SCORE3�5
r�
L;
'7
9
/V
1 41