NC0023906_Lab analysis_20071231

Comments:
MAIL ORIGINAL TO:
Test Initiation Date/Time 7/24/2007 2:00 PM Avg Wt/Surv. Control 0.495
% Survival 97.5
Avg Wt (mg)0.482 Hatch Date: ABS7/23/07['
% Survival 92.5 Hatch Time: 12:30-15:30
Avg Wt (mg)0.527[
% Survival 97.5
Avg Wt (mg)0.493
% Survival 92.5
Avg Wt (mg)0.484
[
% Survival,92.5
Avg Wt (mg)0.485[
% Survival 97.5
Avg Wt (mg)0.568
2
10
10
0.461
10
10
0.499
9
10
0.579 I
2
7.6 / 7.7
7.7 / 5.7
3
7.9 / 7.6
9
10
0.504
Dilution H2O
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Overall Result
ChV |>10Q
Cultured In-House
Outside Supplier
Laboratory: PACE Analytical
2225 Riverside Drive
Asheville NC 28S04
Normal
Hom. Var.
NOEC
LOEC
ChV
Method
Test Organisms
10
10
0.572
10
10
0.515
0
7.7 / 7.4
7.3 / 5.4
25.3 / 24,8,
0
7.7 / 7.8
8.0 / 5.7
46.00
42,00
120
10
10
0.511
10
10
0.555
8
10
0.422
9
10
0.508
1
9
10
0.464
2
7/24/2007
10
10
0.560
9
10
0.444
4
7.6 / 7.5
______________ 7,7 / 5.4
25.2 / 24.8|25.2 / 24.6|24.5 /
4
8.0 / 7,8
7.9 / 5.5
10
10
0.550
9
10
0.438
9
10
0.445
10
10
0.465
10
_10_
■0.475
__3
10
10
0.515
10
10
0.484
10
10
0.599'
4
10
10
0.488
Stats
Cone.
45
65
90
95
100
1
7/22/2007
24.25hr
64.00
80,00
679
<0.1
2.4
1
7,4 / 7.5
7.2 / 6.4
25,0 / 25.1
1
7.8 / 7,7
8.0 / 6.0
24,75hr
62,00
100.00
611
<0.1
3.1
2
7.6 / 7.8
8?2 / 5.9 8.7 / 6.2
25.25hr
63.00
128.00
573
<0.1
2.2
3
7/26/2007
5
7.6 / 7.1
7.7 / 4.9
% Eff- Repl.
[Control | Surviving#
Original #
Wt/original (mg)
8
10
0.454
rand) ,
x
Growth
Calculated Critical
1 2.41
0 2.41
1 2.41
1-069 2.41
P t 2.41
Facility.City of Wilson
NPDES#: NC0023906
Pipe#: 001
County: Wilson
45| Surviving #
Original #
Wt/onginal (mg)
651 Surviving #
Original #
Wt/original (mg)
901 Surviving #
Original #
Wt/original (mg)
951 Surviving #
Original #
Wt/original (mg)
1001 Surviving #
Original #
Wt/original (mg)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin [25.3 / 24.8|25.0 / 25.l|251~7~
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp, at Receipt (°C)
6
8.1 / 7.8
7.7 / 4.8
25.1 / 24.7
Growth
Fl
Fl
100%
>100
>100
Dunnett's
9
10
0.651
Day
3
7.7 / 7,4
7.8 / 5.8
-Ar.bnvilla Co'-tifir-itjciri I Dr;
NC Wastewater 40
NC Drinking Water 37712
SC 99030
FL aT-5 ^9^18
Calculated
-1.169
-0.29
-0.056
-0.083
- Cferlotf^Celfification IE s
—NC-Wastewater-----12_
NC Drinking Water 37706
SC 99006
FL NELAP E87627
race Analytical services, inc.
— Asheville. NC 2880,4
Phone: [phone]
Fax: [phone]
Envh-onnient.nl Sciences Bi
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Jr Hunte^lle. M 280'78
\^PaceAnalyKar p,,0SkiS^
www.pacelabs.com
Signature of Operator in Responsible Chgrgt
Signature of Laboratory Supervisor (x
6
7.7 / 7,4
______ _ 7.8 / 5.0
24.6|25.4 / 24.8|25.1 / 247
5
8.0 / 7.7
_______________7,9 / 5.4
25.2 / 24.6 24,5 / 24.6 25.4 / 24.8
Survival
Fl
Fl
100%
>100
>100
Dunnett's
Survival
Critical
2.41
2,41
’ ___241
2.41
_ _______ 2.41
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
'H aCCOa-
nelac
Comments:
MAIL ORIGIN AL TO:
Test Initiation Date/Time 8/7/2007 2:00 PM Avg Wt/Surv. Control 0.526
% Survival 97.5
Avg Wt (mg)0.512 Hatch Date: ABS8/6/07
[% Survival 95.0 Hatch Time: 12:30-15:30
Avg Wt (mg)0.451
[% Survival 95.0
Avg Wt (mg)0.467
% Survival 100.0
Avg Wt (mg)0.495
L % Survival 100.0
Avg Wt (mg)0.494
[% Survival 95.0
Avg Wt (mg)0.484
iBHli]
/
/
I
10
10
0.515
100[ Surviving #
Original #
Wt/original (mg)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp, at Receipt (°C)
Dilution H2O
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
901 Surviving #
Original #
Wt/original (mg)
951 Surviving #
Original #
Wt/original (mg)
651 Surviving #
Original #
Wt/original (mg)
45[ Surviving #
Original #
Wt/original (mg)
Cultured In-House
Outside Supplier
Normal
Hom. Var.
NOEC
LOEC
ChV
Method
Laboratory: PACE Analytical
2225 Riverside Drive
Asheville N’C 28804
Facility:City of Wilson
NPDES#: NC0023906
Pipe#: 001
County: Wilson
Test Organisms
r
r
Overall Result
ChV |>100
10
10
0.526
Signature of Operator in Responsible Charge,
Signature of Laboratory Supervisor x
8
10
0.413
45.00
44.00
135
9
10
0.518
10
10
0.459
10
10
0.457
10
10
0.431
9
10
0.452
10
10
0.505
10
10
0.488
10
10
0.475
10
10
0.439
2
10
10
0.561
24.75hr
63.00
126.00
638
<0.1
3.0
10
10
0.494
10
10
0.486
10
10
0.496
8
10
0.448
10
_10_
0.506
3
9
10
0.509
10
10
0.539
10
10
0.445
4
10
10
0.550
Stats
Cone.
45
65
90
95
100
2.41
2.41
2.41
2,41
2.41
0
7.5 / 7,2
8.0 / 6.4
25.3 / 25.0
0
7.6 / 7,8
8.6 / 6.4
25.3 / 25.0
1
8/5/2007
25hr
64.00
112.00
662
<0.1
3.2
1
10
10
0.430
1
8.0 / 7,5
8.1 / 6.3
25.2 / 25.0
1
7.8 / 7.7
8.8 / 5.8
25.2 / 25.0
2
8/7/2007
23.75hr
61.00
104,00
669
<0.1
2.9
2
7.5 / 7,2
8.0 / 6.6
25.4 / 24.8
2
7.6 / 7.8
6.8 / 6.4
25.4 / 24.8
3
8/9/2007
% Eff. Repl.
[Control j Surviving #
■ Original #
Wt/original (mg)
Asheville, NC 288V4
Phone: [phone]V6
Fax: [phone]
Growth
Calculated Critical
17,5
15.5
20
20
16
X
7- — v
Environmental Sciences Branch
Division of Wa/er Quality
NC DEN R
1621 Mail Service Center
Raleigh. NC 27699-1621
6
7.4 / 7.5
8.0 / 6.2,
25.4 / 24.8
6
8.0 / 7,6
8.5 / 6.2
25.4 / 24.8
4
7,7 / 7.0 j
7.6 / 5.0
25.3 / 24.7
4
7.7 / 7.6
7.4 / 4,6
25.3 / 24.7
Survival
|F |
Fl
100%
>100
>100
Steel’s
5
7.8 / 7.4
6.8 / 5.1
25.1 / 25.2
5
7.7 / 7.8
7.2 / 5.7
25.1 / 25.2
Growth
Fl
Fl
100%
>100
>100
Dunnett’s
Calculated
2.216
1.627
0.629
0.659
■ehartottc Ccftation IDs
4MC Wastewater-----45—
NC Drinking Water 37706
SC 99006
FL NELAP E87627
Asheville Certification IDs
NC Waste water 40
NC Drinking Water 37712
SC 99030
FL NEEAPQ Form AT&'/'&ffill)
Survival
Critical
______10
______10
______10
____10
______ 10
REPORT QF I QRnnflTnRY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services. Inc.
10
10
0.472
Day
3
7.2 / 7.1
8.0 / 6.0
25.1 / 25.1
3
7.5 / 7.8
7.8 / 6.5
25.1 / 25.1
— y.cty Report Form-Chronic Fathead
Hunter^e, NC 28078
^Pace Analy^al fS^04.875.9091
www.pacelabs.com
Comments:
MAIL ORIGINAL TO:
Test Initiation Date/Time 9/11/2007 2:35 PM Avg Wt/Surv. Control 0.781 Test Organisms
’ % Survival 97.5
Avg Wt (mg)0.760 Hatch Date: ABS9/10/07
[% Survival 100.0 Hatch Time: 12:30-15:30
Avg Wt (mg)0.782
% Survival 100.0
Avg Wt (mg)0.931
% Survival 100.0
Avg Wt (mg)0.918
% Survival 97.5
Avg Wt (mg)0.984
% Survival 100.0
Avg Wt (mg)0.814
]
iKfiwlHwnw
1001 Surviving #
Original #
Wt/original (mg)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp, at Receipt (°C)
Dilution H2O
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
901 Surviving #
Original #
Wt/original (mg)
951 Surviving #
Original #
Wt/original (mg)
45| Surviving #
Original #
Wt/original (mg)
Cultured In-House
Outside Supplier
651 Surviving #
Original #
Wt/original (mg)
Normal
Hom. Var.
NOEC
LOEC
ChV
Method
Laboratory: PACE Analytical
2225 Riverside Drive
Asheville NC 28804
Facility:City of Wilson
NPDES#: NC0023906
Pipe#: 001
County: Wilson
E
F
Overall Result
ChV |>100
Pace Inc.
__ 2225 Riverside Drive
Asheville. NC 28^04
Phone: [phone]i76
Fax: [phone]
44.00
45.00
124
10
10
0.762
10
10
0.886
10
10
0.919
10
•10
0.841
10
10
1.059
10
10
0.865
10
10
0.902
10
10
0.742
10
10
1.043
10
10
1.110
2
10
10
0.718
10
10
0.762
10
10
0.780
10
10
1.016
10
10
0.841
3
10
10
0.808
10
10
0.746
9
10
0.892
10
10
0.963
10
10
0.921
10
10
0.799
4
10
10
0.773
Stats
Cone.
45
65
90
95
100
Survival
Critical
______10.
10
______10
______10.
10
1
9
10
0.742
1
7.8 / 7.1
8.2 / 5.2
24.9 / 25.3
1
7,6 / 7.7
9.0 / 6.2
24.9 / 25.3
2
9/11/2007
7.6 / 7.7
8.6 / 5.0
25.2 / 24.6
% Eff. Repl.
[Control | Surviving #
Original #
Wt/original (mg)
24.5hr
64.00
112.00
670
<0.1
2.2
24.25hr
61.00
104.00
607
<0.1
3.3
24.25hr
63.00
126.00
707
<0.1
2.6
REPORT OF LABORftTOF
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
ndac
Signature of Operator in Responsible Charge
Signature of Laboratory Supervisor'' x
Growth
Calculated Critical
2.41
2.41
2.41
2.41
2.4
20
20
20
18
20
LYSISAsheville Certification IDs
NC Wastewater TO
NC Drinking Water 37712
SC 99030
FL NEL/3^/q f0rm
6
7.6 / 7.8
7.9 / 4,8
25.3 / 24.6
6
7.7 / 7.8
9.0 / 5.3
25.1 / 24.7
4
7.2 / 7.2
8.5 / 5.1
25.0 / 25.0
4
7.7 / 7.7
9.0 / 5.5
24.5 / 24,6
Survival
Fl
F|
100%
>100
>100
Steel’s
0
7.5 / 7.6
8.2 / 5.6
25.0 / 24,8
0
7.5 / 7.6
9.0 / 6.0
25.0 / 24.8,
1
9/9/2007
5
7.6 / 0.2
8.0 / 4.9
25.1 / 24.8
5
7.6 / 7.7
9.3 / 4.8
25.4 / 24.8
Growth
Fl
Fl
100%
>100
>100
Dunnett's
2
7.7 / 7.4
8.0 / 6.3
25.1 / 24,9[ 25.3 / 25.2
2 3
7.8 / 7.9
8.0 / 6.7
25.1 / 24.9
3
9/13/2007
X
Environmental Sciences Bhtnch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh. NC 27699-1621
10
10
0.866
Day
3
7.6 / 7.6
8.0 / 5.8
ffMcicity Report Form-Chronic Fathead
Huntej^le. NC 28078
ticeAnalymr
www.pacelabs.com
Calculated
-0.378
-3.016
-2.791
-3.963
■Charlotte Cert^ication IDs
t4€-Wastewater-----T9-----
NC Drinking Water 37706
SC 99006
FL NELAP E87627
Effl
Comments:
MAIL ORIGINAL TO:
Test Initiation Date/Time 10/9/2007 1:35 PM Avg Wt/Surv. Control 0.589 Test Organisms
% Survival 97.5
Avg Wt (mg)0.572 Hatch Date: ABS10/8/07
[% Survival 100.0 Hatch Time: 12:30-15:30
Avg Wt (mg)0.615
[% Survival 95.0
Avg Wt (mg)0.528
[% Survival 95.0
Avg Wt (mg)[0.610|
% Survival 100.0
Avg Wt (mg)0.641
% Survival 100.0
Avg Wt (mg)0.621
[iTHlffi
1001 Surviving #
Original #
Wt/original (mg)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp, at Receipt (°C)
Dilution H2O
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
45| Surviving #
Original #
Wt/original (mg)
901 Surviving #
Original #
Wt/original (mg)
95l Surviving #
Original #
Wt/original (mg)
Pace Anafy^a1'SSrlr‘i£'ffs, Inc.
2225 Riverside Drive
Asheville. NC 28804
Phone: [phone]i76
________Fax- 828.252.4618
651 Surviving #
Original #
Wt/original (mg)
Normal
Hom. Var.
NOEC
LOEC
ChV
Method
Laboratory: PACE Analytical
2225 Riverside Drive
Asheville NC 28804
Facility:City of Wilson
NPDES#: NC0023906
Pipe#: 001
County: Wilson
Overall Result
ChV | >100
0
6.9 / 7.4
7.0 / 6.1
24.8 / 25.1
0
7.6 / 7.8
9.0 / 6.8
24.8 / 25.1
1
10/7/2007
10
10
0.582
9
10
0.579
10
10
0.694
10
10
0.726
10
10
0.585
38.00
41.00
122
1
7.5 / 7.3
7.9 / 6.0
25.2 / 25.0
1
7.6 / 7.5
8.8 / 5.0
25.2 / 25.0
2
10/9/2007
10
10
0.610
2
9
10
0.585
10
'l_0_
0.622
10
10
0.565
9
10
0.585
10
10
0.678
3
10
10
0.578
10
10
0.571'
10
10
0.496
10
10
0.648
10
10
0.600
10
10
0.552
9
10
0.471
9
10
0.515
10
10
0.628
4
10
10
0.585
10
10
0.683
Stats
Cone.
45
65
90
95
100
2.41
2.41
2.41
2.41
2.41
24.25hr
50.00
80.00
644
<0.1
2.2
1
10
10
0.541
25.25hr
55.00
83.00
600
<0.1
3.4
% Eff. Repl.
[Control | Surviving #
Original #
Wt/original (mg)
24,75hr
54.00
84.00
617
<0.1
3.5 Growth
Calculated Critical
__20
16
___16
___20
20
2
7.5 / 7.2
8.3 / 7.0
24.9 / 24.8
2 '
7.6 / 7.4
8.1 / 6.3
24.9 / 24.8
3
10/11/2007
Survival
Critical
10
10
____10
______10
______________ 10
REPORT OF I ARnRATllRY ANfllYSlS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
nelac
Asheville Certification IDs
MU Wastewater TO
NC Drinking Water 37712
SC 99030
^^WQFormA¥-^4)
4
7.3 / 7.2
8.4 / 8.0
25.3 / 25.0
4
7.5 / 7.4
7.9 / 7.6
25.3 / 25.0
5
7.2 / 7.2
8.1 / 4.8
25.1 / 24.9
5
7,6 / 7,6
7.8 / 4.2
25.1 / 24.9
Survival
Fl
Fl
100%
>100
>100
Steel's
6
7.4 / 7.3
8.1 / 5.8
25.3 / 24.8
6
7.6 / 7.3
8.8 / 6.2
25.3 / 24.8
Growth
Fl
Fl
100%
>100
>100
Dunnett's
Calculated
-1.067
1.114
-0.957
-1.728
-1.229
CTTarldtte CgniflcatloiTTDs
NC Wastewater T2
NC Drinking Water 37706
SC 99006
FL NELAP E87627
Signature of Operator in Responsible Charge^
Signature of Laboratory Supervisor x )
Environmental Sciences-Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Htj^xicity Report Form-Chronic Fathead
y A Huntersvj^lC 28078
A ^.^1, ^4-:JW/® Phone:9092face AnalytiCdl 704.875^91
www.pacelabs.com
10
'10
0.670
Day
3
7.3 / 7.1
8.4 / 7.5
24.8 / 24.8
3
7.5 / 7.3
8.2 / 6.4
24.8 / 24.8
r Cultured In-House
P , Outside Supplier
x
E-nl mi
Date:August 24, 2007
NPDES #: NC- 0023906 Pipe #: 001 County: Wilson
Comments: .
North Carolina Ceriodaphnia Chronic Pass/l-ail Reproduction Toxicity Test Percent
L L L
90%
Pass Fail
X
Test Start Date: 08-08-07
Sample 2 08-09-07
a
Duxntion
23-h
24 75-h
.W. 3 3
g
Control
High Cone.
DO (ing/L)7-days pH (S.U.)Ceriodaphnia dubia Duration:Organism Tested:
Concentration (%)
Mortality (7c)
D.O. (mg/L)
Control
Treatment 2 Sample 1
Sample 2
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO3/Li
Conductivity (pnihos/cin)
Total Residual Chlorine (ing/Lj
Sample Temp, al Receipt CC)
Chronic Test Results
Calculated t:
Tabular t:
% Reduction:
PO Box 7565
Asheville, NC 28802
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh. NC 27699-1621
5
pH (S.U.)
Control
Treatment 2
Treatment 2
34.9
ci
f
5
■A
I
•o s
1
II
5
Average
Reproduction
Control
25.7
Control Organisms__________
Number of Young Produced
Adult Survival: (L)ive. (D)ead
Effluent Percentage
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
7.7
7.9
7.24
7.81
1
35
L
2
36
L
7.29
7.84
4
34
L
7.7
7.5
5
34
L
7.23
7.79
6
38
L
6
26
L
8
27
L
9
24
L
9
37
L
10
26
L
10
34
L
11
27
L
Project:
Samples:
Mortality
Control
0.0
3371
070808.13.070811.05
8.0
7.7
3
33
L
7
27
T
7
32
L
8
34
L
11
37
L
12
23
L
s, Inc.
Phone: [phone]
1st Sample
7.17
773
1st Sample
7.32
7.80
12
35
T"
1 st Sample
7.8
7.7
1st Sample
7.6
7.8
2nd Sample
7.36
7.80
2nd Sample
7.6
8.4
605
<0.10
0.6
702
<0.10
1.1
Treatment 2
0.0
Control CV
40.45
165. IW.
161
5
25
L
-13.328
2.508
-36.0
6.3
*• control
proihr mg 3rd brood
100
E^dronmental Testing Soluti^i
iolutions, Inc. ---------—------
Sample Type/Duration
Grab Comp.
X-
Method of Determination
Trimmed Spearman Karber
Pi-obit
Other:
LCjo/Acute Toxicity Test
(Mortality expressed as %. combining replicates.)
LC50 =
95% Confidence Limits
to
12 3 4
26 | 28 | 26 23
L
Collection (Start) Date:
Sample 1 08-06-07
il
This data package contains the toxicity test results summarized on this form and includes a Cove}- Page.
AT-1 Form. Laboratory Benchsheets. Statistical Analyses. Chain-of-Custody Forms and Quality Control Charts.
DWQfonn AT-1 (3/87) rev. 11/95
Facility: Tritest, Inc.
Wilson WWTP
Laboratory Performing Test: Environmental Testin;
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor:
Effluent Toxicity Report Form - Chronic Pass/Faii and Acute LC50
Date
NPDES#NCCC23906|>e #
Comments
X
MAIL ORIGINAL TO
1 2 3 4 .5 6 7 8 9 10 11 12
# Young Produced 29 27 25 29 2830 29 29 29 30 35 31 % Mortality Avg. Reprod.
L L L L L L L L L L L LAdult (L)ive (D)ead
90.0%Effluent %0%27.2
Treatment 2 Treatment 2
Control CV
8.1%TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
21# Young Produced 30 28 30 24 29 27 27 26 30 27 27 % 3rd Brood PASS FAIL
XLLLLL L LL L L L L 100%Adult (L)ive (D)ead
Complete This for Either Test Test Start Date
Collection fStart) Date 8-8-07
pH 1st sample 1st sample 2nd sample Sample 1 8-6-2007 Sample 2 8-9-2007
Control 7.9 8.0 7.8 8.0 7.9 7.6 Sample Type (Duration)
8.2 7.9Treatment 2 8.4 8.3 8.2 8.2 Grab Duration 1st 2nd
Sample 1 24hrs.Tox Tox
XendendSample 2startendstartstart 24hrs.Dilution Sample Sample
D.O.•W46.22nd sample1st sample 1st sample Hardness (mg/L)
8.6 8.7 8.7 8.7 8.8 8.5Control 176 611 685Spec. Cond. (pmhos)
8.9 8.7 8.8 8.7 8.7 8.5Treatment 2 0.09 <0.05Chlorine (mg/L)
2.4 1.7Sample Temp, at receipt (°C)
LCSO/Acute Toxicity Test
(Mortality expressed as %. combining replicates)
Concentration
Mortality starUend start/end
Method of DeterminationLC50 =Control
Probit95% Confidence Limits Moving Average High Cone.
%Other PH D.O.Spearman Karber
ICeriodaphnia dubiaOrganism Tested
DEM Form AT-1
ETT Environmental, Inc.
16-Aug-07
County: WILSON
2.024
2.508
7%
0%
Control
29.3
Conti. I
Comp
T"
Environmental Sciences Branch
wiv. vi moici wuaiiiy
IX.U'L.IVIX
lux. i man jci *iuc x^ciilci
t^aiciyiij ixviiti \>aivinia Aiuoa-iuAi
Chronic Test Results
Calculated t=
Critical Value=
% Reduction=
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Facility; WILSON EFFLUENT^
Laboratory Performing Test:
Signature of Operator in Responsible Charge
_____________________
Signature of Laboratory Supervisor
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
Date 17-May-C7
County: WILSONNPDES#Pipe #
Comments
MAIL ORIGINAL TO
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
4 '3 5 6 7 812 9 10 11 12
32 30 30 2326293031 28 29# Young Produced 31 33 % Mortality Avg. Reprod.
L L LLILL L LL L LAdult A live (P}oad
90.0%Effluent %0%29.8
Treatment 2 Treatment 2
Control CV
9.2%TREATMENT 2 ORGANISMS 4 5 6 7 8 912 3 10 11 12
30 29 31 31 32 31 28 28 30# Young Produced 30 28 30 % 3rd Brood PASS FAIL
XLLLLLLLLLLLL100%Adult (L)ive (D)ead
Complete This for Either Test Test Start Date
Collection (Start) Date 09-May-07
PH 2nd sample Sample 11st sample 5-7-20071st sample Sample 2 5-10-2007
8.57.6 7.57.8 8.0 7.8Control Sample Type (Duration)
7.87.7 7.7 7.47.7 7.9Treatment 2 Grab Duration 1st 2nd
Sample 1 24hrs.Tox Tox
XendSample 2endstart Dilution Sample Sampleendstart24hrs.start
D.O.44.12nd sample Hardness (mg/L)1st sample1st sample
8.7 8.48.5 8.9 630 5669.1 8.6 201ControlSpec. Cond. (pmhos)
9.2 8.49.0 0.08 0.079.3 8.5 8.5Treatment 2 Chlorine (mg/L)
0.11.9Sample Temp, at receipt (°C)
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
Concentration
Mortality start/endstart/end
Method of DeterminationLC50 =Control
Probit High Cone.Moving Average95% Confidence Limits
D.OOtherpH%
Organism Tested
DEM Form AT-1
I
-0.57
2.508
-2%
• .J
Control
0%
Control
Comp
X
Environmental Sciences Branch
miv. vi naici \xi«ciiiiy
It. '-*•
IU4I iViail UCIVILC VrCtllCI
naiciyii, iiuiui vaiuima 1.1 uja-ivt
Chronic Test Results
Calculated t=
Critical Value=
% Reduction=
ETT Environmental, Inc.
Spearman Karber
Ceriodaphnia dubia
Effluent ToxiciM^port Form - Chronic Pass/Fail and Acu|^_C50
Facility: WILSON HOMII^^OREEK WWTF npdes# NCo^Soe
Laboratory Performing Test:
x /f -~
Signature of Operator in Responsible Charge
X ________________________
Signature 'of Laboratory Supervisor
NPDES#NC 0023906
County Wilson
Comments
OviAIL ORIGINAL TO:
0.158
North CarolinaCor/oc/ap/in/a Chronic Pass/Fail
CONTROL ORGANISMS 1 2 7 8 ;9 10 11 12
tf- Young Produced 23 23 28 24 19 21 26 24 23 27
Adult (L)ive (D)ead L L L L L L L L L L
Effluent%90
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 PASS FAIL
# Young Produced 21 18 25 26 22 25 22 28 23 26 X
Adult (L)ive (D)ead L L L L L L L Check OneLLL
^Complete This For Either Test |1st 1st 2nd
ControlPH 7.7 7.6 8.0 7.9 8.0 Collection Start Date8.1
Treatment 8.0 8.0 Sample 18.0 Sample 25\7\07 5\10\07
Samoel Tvoe/Duration
Grab Comp. Deration
Sample 1
X 23.75hr
Sample 21st1st2nd X 23.75hr
D O Control 8.0 7.8 8.0 7.8 8.4 8.1 Hardness (mg/|)
43
Treatment 8.2 8.0 8.3 7.9 Spec Cond. (umhos)8.5 8.3 503 49S
Chlorine (mg/l)
<0.1 <0.1
Sample temp at receipt fC)
1.6 2.4
%%%%%%%%
Concentration%%%%%%%%
Mortality
LC50= %Method of Determination start/end start/end
95% Confidence Moving Avg.Probit Control
% -%Spearman Other
PH LWOrganism Tested: Ceriodaphnia dubia
E
n
d
E
n
d
Test Start Date
5\9\07
Tabular t
% Reduction
0.00
Treatment 2
0.0
Cnntrnl CV
11.3 I
Pace Analytical Services, Inc.
2225 Riverside Drive
Asheville, l\IC 28804
Phone: [phone]
Fax: [phone]
High
Cone
%control organisms
producing 3rd brood
f 83.3
I
E
n
d
wee Analytic^
WWW. I
__¥
Mole Please
Complete This
Section also
2,552
0.84
LCSO/Acute Toxicity Tesl
(Mortality expressed as % combinino replicates)
%
I % Mortality
Control
| Avg. Reprod.
Control
23.80
Treatment 2
23.60
7.9
s t
I
a
8,1
s t
t
a
78
s t
t
a
r
Test Duration (Hours): .
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services. Inc.
nehc
■=6^
tn —<
—• cn
Reproduction Toxicity Test
3 4 5 6
a
—i S
!
Chronic Test Results
Calculated t
120
EPA blocking method used. No organisms in replicates
4&5 had offspring. Not male but looked unhealthy
Not included mstalistical analysis Sample not aerated
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh. North Carolina 27699-1621
Facility: Wilson___________
Hominy Creek WTF
Laboratory Jferforming Test PACE Analytical Services, Inc
Signature of Operator in Responsible Charge
i l
Sinnaiiirp of I ahoratnrvSmi^rVi'snr ~ 7~'
Pace Analytical Services, Inc.
9800 Kincey Avenue, Suite 100
Huntersvill^C 28078
’ Phone: 71^^5.9092
Fax: [phone]
Report AT-1 Form Chronic Pass/Fail and Acute LC50
Date 5\22\07
Asheville Certification IDs
NC Wastewater 40
NC Drinking Water 37712
SC 99030
FL NELAP E87648
Charlotte Certification IDs
NC Wastewater 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627
Facility: Wilson NPDESSNC 0023906 Pipe #001
County WilsonJnc.
2.562
7 8o 9 10 11 12
ff Young Produced 26 28 28 16 30 33 33 17 29 27 26 32
Adult (L)ive (D)ead L L L L L L L L L L L L
Effluent%90
TREATMENT 2 ORGANISMS 1 2 3 4 10 11 12 PASS FAILtr Young Produced 22 23 22 22 20 28 28 XAdult (L)ive (D)ead L L L L L L Check OneL
[Complete This For Either Test ~|1st 2nd
pH Control Collection Start Dale
Treatment Sample 1 Sample 2 8\9\07
Comp. Duration
Sample 1 X 23hr
1st 1st Sample 2 X 24 75hrD.O Control 8.0 7.8 8.1 7.8 Hardness (mg/1)45
Treatment 7.6 7.4 8.1 7.8 Soec. Cond. fumhos)
133 557 595
Chlorine (mg/1)
<0.1 <0.1
Samole temo at receiot (C)
3.0%%%%%%%%
Concentration%%%%%%%%
Mortality
LC50= 0/ /o Method of Determination start/end start/end95% Confidence Moving Avg.Probit Control
% -%Spearman Other
PH DTP
s t
I
a
S t
t
a
%
%control organisms
producing 3rd brood
r 91.7
High
Cone.
E
n
d
Tabular t
% Reduction
0.00
Treatment 2
00
Cnntrnl CV
20.4 I
Pace Analytical Services, Inc.
2225 Riverside Drive
Asheville, NC 28804
Phone: [phone]
Fax: [phone]
E
n
d
E
n
d
I
7;
S'
X (/)
CD
CD
2.508
173
LC50/Acu!e Toxicity Test
hMortalitv expressed as % comhinino replicates)
%
% Mortality
Control
27.10
Treatment 2
22.40
8\6\07
Samoel Tvoe/Duration
Grab
3,8
Note: Please
Complete This
Section also
Samples Not Aerated Unless Otherwise Noted
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
{Test Start Dale- T
8\8\07
h-li
Test Duration (Hours)-
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
»• *C-O»„
,-r nzM'-ir**
Chronic Test Results
Calculated t
| Avg Reprod.
Control
5 6 7 8 9
03333
North Carolina CeriodaphniaChronic Pass/Fail Reproduction Toxicity Test
CONTOHt. ORGANISMS 12 3 4
Labora^ry Performing Test , PACE Analytical Service
I
signature of Operator in Responsible Charge ' ~ “
I----‘ V-:/ a-X Xzz i •>
SinnafijrR nf I ahrtralnry £< mpn/jsnr ; ' /
“ cT" :—
MAIL ORIGINAL TO:
organism Tested: Ceriodaphnia dubia
Asheville Certification IDs
NC Wastewater 40
NC Drinking Water 37712
SC 99030
FL NELAP E87648
■1st 1st 2nd
LLL-LeJ _Z-4 I 7,5 I P7.4 I 7,4 J
I-8-1 81 I 8.1 | 8,0 | | 7.9 I 8.0 |
s t -------------—■—1
1
a
2nd
J 8.0 7,7
J I 8.5 8,2 I
Pace Analytical Services. Inc.
9800 KinceyAvenue. Suite W0
A Hunters, NC 28078
/I HQ l\ Phon^n)4.875.9092
uUu laiyilUdl Fax: [phone]
mpfitoaWoxicity Report AT-1 Form Chronic Pass/Fail
Comments
Stats calc.sig difference but %reduction was
<20% so test was a PASS
Charlotte Certification IDs
NC Wastewater 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627