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HomeMy WebLinkAboutNC0023906_Lab analysis_20061231Date 17-Nov-06 County: WILSONNPDES#NC002390('ipe # Comments MAIL ORIGINAL TO 1 2 3 4 5 6 7 8 9 10 11 12 H Young Produced 30 27 25 21 30 29 29 29 24 26 27 22 % Mortality Avg. Reprod. Adult (L)ive (D)ead L L L L L L L L L L L L Effluent %90.0%0%24.4 Treatment 2 Treatment 2 Control CV TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 11.5%10 11 12 # Young Produced 20 25 27 25 21 26 29 24 25 24 20 27 % 3rd Brood Adult (L)ive (D)ead L L L L L L L L L L L L 100% Complete This for Either Test Test Start Date Collection (Start) Date 11-8-06pH1st sample 1st sample 2nd sample Sample 1 11-6-2006 Sample 2 11-9-2006 Control 7.3 7.5 7.7 7.7 7.6 7.6 Sample Type (Duration) Treatment 2 7.5 7.7 7.3 8.1 7.8 7.6 Grab Duration 1st 2nd Sample 1 24hrs.Tox Tox start end start end start end XSample 2 "24hrs.Dilution Sample Sample D.O.1st sample 1st sample 2nd sample Hardness (mg/L)46.0 Control 8.8 8.7 8.7 8.5 8.4 8.3 Spec. Cond. (pmhos)552 485 Treatment 2 8.7 8.7 8.7 8.5 8.4 7.6 Chlorine (mg/L)0.08 <0.05 Sample Temp, at receipt (°C)2.1 2.7 LC50/Acute Toxicity Test start/end start/endLC50 = Control 95% Confidence Limits ProbitMoving Average High Cone. %Spearman Karber pH D.O. Organism Tested J DEM Form AT-1 ETT Environmental, Inc. 1.797 2.508 8% 0% Control 26.6 Control |Other Ceriodaphnia dubia Comp T" Chronic Test Results Calculated t= Critical Value= % Reduction= Environmental Sciences Branch wi». ui uaici wuaiity IW.V*. LJL.t'IIX lux i mail <jci viw ^viuci lAaicikjn, iivilii ^aivinia A/u^a-iv^i North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test CONTROL ORGANISMS PASS FAIL TF (Mortality expressed as %, combining replicates) ~lll III FR Method of Determination 183 , Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 .-acility: WILSON WWTF Laboratory Performing Test: Signature of Operator in Responsible Charge 2S____ _______ Signature of Laboratory Supervisor Inc.En^onmental Testing Solutio, November 24, 2006Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC5o Date: County: WilsonNPDES#: NC- 00'23906 Pipe #: 001 Comments: g/c-/00(55^ North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent LLLLL FailPass XLLLLLLLLL 11-09-06Sample 2 Ehirnion W-)i 24-h ’? 33 ____ Control Higli Cone. DO (nig/1)pH (S.U.)7-daysDuration:Ceriodaphnia dubiaOrganism Tested: PO Box 7565 Asheville, NC 28802 D.O. (mg/L) Control Treatment 2 Sample 1 Sample 2 Alkalinity (mg CaCO3/L) Hardness (nig CaCOj/L) Conductivity (jimhos/cm) Total'Residual Chlorine Ung/L) Sample Temp. atReceipt (°C) Phone: (828) 350-9364 Fax: (828) 350-9368 E-mail: JimSumner@ aol.com Chronic Test Results Calculated t; Tabular t: % Reduction: Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center Raleigh, NC 27699-1621 Concentration (%) Mortality (%) 3 a pH (S.U.) Control Treatment 2 Treatment 2 35.8 I I I 3 55 3I J 3 3 2 31 L 8.3 8.1 31 L Project: Samples: 2857 061108.04, 061111.04 Control Organisms_________ Number of Young Produced Adult Survival: (L)ive, (D)ead 8.1 8.4 7.8 8.4 9 32 L 10 11 30 L 12 30 L Mortality Control 0.0 Average Reprodnction Control 30.0 461 <0.10 0.4 1 30 L 1st Sample 7.2917.66' 7.80 8.09 1st Sample 8.2 8.4 1st Sample 7.6 8.4 1st Sample 7.55 7.39 8.03 7.85 -9.324 2.508 -19.4 553 <0.10 0.7 Treatment 2 0.0 Control C V 4.9 7. control orjwttsms prottarinf 3rd brood 100 3 4 5 6 7 8 28 31 28 29 32 28 T LCjo/Acute Toxicity Test (Mortality expressed as %, combining replicates.) Method of Determination Trimmed Spearman Karber Probit Other: LC50 = 95% Confidence Limits to 12 3 37 | 37 | 35 L 45'678 36 | 37 | 33 | 38 | 35 L Sample Type/Duration Ck»l> Comp. x 44. 42 .64 149. 153 _____ 2nd Sample 7.75 17.36 7.92" 7.79 2nd Sample 8.2 8.4 Facility: Tritest. Inc. Wilson WWTP Laboratory Performing Test: Environmental Testing, Solutions, Inc. Signature of Operator in Responsible Charge: ----- Signature of Laboratory Supervisor: -------------- 9 IQ 11 12 35 | 35 | 38 | 34 T 11 Collection (Start) Date: Sample 1 11-06-06 This data package contains the toxicity test results summarized on this form and includes a Cover Page, AT-I Form, Laboratory Benchsheets, Statistical Analyses, Chain-of-Custody Forms and Quality Control Charts. DWQ form AT-1 (3/87) rev. 11/95 Effluent Percentage 90% Treatment 2 Organisms Number of Young Produced Adult Survival: (L)ive, (D)ead Test Start Date: 11-08-06 Date 22-Aug-06 County: WILSONNPDES#NC0023906 ie # Comments MAIL ORIGINAL TO North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test CONTROL ORGANISMS 725 6 8 9 10 11 12134 Avg. Reprod.23 22 27 22 31 28 23 % Mortality# Young Produced 26 21 27 28 25 LLLLLL L L L L LLAdult (L)ive (D)ead 19.917%90.0%Effluent % Treatment 2Treatment 2 Control CV 12.2%5 6 7 8 9 10 11 12TREATMENT 2 ORGANISMS 1 2 3 4 25 % 3rd Brood21918252122 025282421# Young Produced D 100%D L L L L LLL L L LAdult (L)ive (D)ead Test Start DateComplete This for Either Test Collection (Start) Date 09-Aug-06 pH Sample 2 8-10-20062nd sample Sample 1 8-7-20061st sample1st sample 7.5 7.47.8 7.5Control7.9 8.0 Sample Type (Duration) 7.7 7.67.8 7.77.8 7.9Treatment 2 1st 2ndGrabDuration Tox ToxSample 1 24hrs. X Sample SampleSample 2 24hrs.Dilutionendendendstartstartstart D.O.2nd sample 46.1Hardness (mg/L)1st sample 1st sample 562 5968.0 8.48.2 8.5 8.18.1Control Spec. Cond. (pmhos) <0.05<0.057.9 8.48.7 8.08.6Treatment 2 8.9 Chlorine (mg/L) 2.1 0.8Sample Temp, at receipt (°C)- V-v - LCSO/Acute Toxicity Test (Mortality expressed as %. combining replicates) Concentration start/endstart/endMortality Method of DeterminationLC50 =Control Probit High Cone.Moving Average95% Confidence Limits D.O.pHOther% JOrganism Tested DEM Form AT-1 ETT Environmental, Inc. Spearman Karber Ceriodaphnia dubia 113 109 21% 0% Control 25.3 Control Comp T" Environmental Sciences Branch miv. ui naici wuaiiLy U/U.I’IIX IUA I IViail UCIVILC rxaigivpi, iiuiui v*aii/iiiia a/ujj-iuai Chronic Test Results Rank sum= Critical Value= % Reduction= PASS FAIL ITT Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 ucility: WILSON HOMINY CF^KWWTF Labor^jory Performing Test: x zA.. Signature of Operator ResponsfoJ<pharge x___________ Signature of Laboratory Supervisor August 21, 2006Effluent Toxicity Report Form - Chronic Pass,Tail and Acute LC50 Date: County: WilsonPipe #: 001NPDES #: NC- 0023906 Comments: North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent 90% FailPass X LL Test Start Date: 08-09-06 7.23 08-10-06Sample 2 DusaUcn 22.75-h 23 75-h 35. 33 Control High Cone. DO (mg/L)pH (S.U.)7-daysDuration:Ceriodaphnia dubiaOrganism Tested: 1 34 L 3 34 L 2 3? T Effluent Percentage Treatment 2 Organisms Number of Young Produced Adult Survival: (L)ive, (D)ead D.O. (mg/L) Control Treatment 2 Sample 1 Sample 2 Alkalinity (mg CaCOyL) Hardness (mgCaCO/L) Conductivity (itmhos/cm) Total Residual Chlorine (mg/L) Sample Temp, at Receipt (°C) Chronic Test Results Calculated t: Tabular t: % Reduction: I I I Concentration (%) Mortality (%) pH (S.U.) Control Treatment 2 LC50 = 95% Confidence Limits to 3 Treatment 2 33.8 I 'TO Box 7565 Asheville, NC 28802 I. rl I Mail Original To: North Carolina Department of Environment and Natural Resources DWQZ Environmental Sciences Branch 1621 Mail Service Center Raleigh, NC 27699-1621 I I 3 3 Contrui Organisms__________ Number of Young Produced Adult Survival: (L)ive, (D)ead 8.1 8.3 7.8 8.2 2_9_ 31 L 11 31 L 11 34 L 12 32 L Project: Samples: 2690 060809.04, 060812.15 Average Reproduction Control 30.230 T- 4 30 L 5 3? L 6 30 L 7 33 L 9 37 T w 30 L 10 30 L 12 33 L Mortality Control 0.0 530 <0.10 0.6 1st Sample 7.341 7.24' 7.68 7.68 7 32 L 45. 43 >». 1’4. 156 -4.615 2.508 -12.2 536 <0.10 0.7 1st Sample 8.0 8.2 1st Sample 7,3617.15" 7.66 7.67 1st Sample 8.1 ”83 8 28 L 8 32 L Treatment 2 0.0 Control CV 4.4 % control orgsasnis producing 5rd brood 100 1 28 L 7.9 8.2 Sample Type/Duration Grab Comp. x” X *(82^ 350-9368 E-mail: JimSiminer@aol.com Method of Determination __Trimmed Spearman Karber Probit Other: LC50ZAcute Toxicity Test (Mortality expressed as %, combining replicates.) 4 5 6 36 | 30 | 37 L lolutions, Inc. 2nd Sample 7.04 7.56 7.58 2nd Sample 8.0 8.1 ii Collection (Start) Date: Sample 1 08-07-06 This data package contains the toxicity test results summarized on this form and includesa Cover Page AT-1 Form, Laboratory Benchsheets. Statistical Analyses. Chain-ofC us tody Forms and Quahty Control Charts_ DWQfonn AT-1 (3/B7) rev. 11/95 Facility: Tritest. Inc. Wilson WWTP Laboratory Performing Test: Environmental Testing^ Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor: 3 29 L Date:May 19, 2006 NPDES #: NC- 0023906 Pipe #: 001 County: Wilson th Carolina Ccriodaphriia Chronic Pass/Fail Reproduction To.viJty Test Pt-lCcHl FailPass X Test Start Date: 05-03-06 7.80 8.02 Sample 2 05-04-06 Du radon 23.5-h 24 h .<5. J3 3 to Control High Cone. Ceriodaphnia dubia Duration:7-days pH (S.U.)DC) (mg/L)Organism Tested: PO Box 7565 Asheville, NC 28302 Environmental Testing Solutions, Inc. Phone: (828) 350-9364 Fax: (828) 350-9368 E-mail: JimSumner@aol.com Sample 1 Sample 2 Chronic Test Results Calculated t: Tabular t: % Reduction: 1 Concentration (%) Mortality (%) D.O. (mg/L) Control Treatment 2 Alkalinity (mg CaCOj/L) Hardness (mg CaCO3/Li Conductivity (pmlios/cut) Total Residual Chlorine (mg/L) Sample Temp, al Receipt (°C) Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center Raleigh, NC 27699-1621 pH (S.U.) Control Treatment 2 I 1 I Treatment 2 31.4 I f •o (S 33 I I Average Reproduction Control 28.8 Control Organisms_________ Number of Young Produced Adult Survival: (L)ive. (D)ead 7.8 8.2 2 33 L 2 31 L 7.79 7.94 7.8 7.8 3 35 L 3 28 L 4 31 L 4 30 L 7.8 7.8 6 28 L 7 29 L 8 34 L 10 32 T u 28 L Mortality Control 0.0 Effluent Percentage 90% Treatment 2 Organisms Number of Young Produced Adult Survival: (L)ive. (D)ead 1 27 L 1 29 L 5 26 L 5 31 L 6 35 L 8 30 L 9 30 L 9 31 L 10 27 L 11 32 L -2.959 2.508 -9.0 1st Sample 7.6 7.5 1st Sample 7,78 7.78 7 29 L 12 29 L 41.41 155. 157. 153 550 <0.10 0.5 1st Sample 7.83 7.68 7.64 Method of Determination ITrimmed Spearman Karber Probit LjOther: ________________ 1st Sample 7.7 7.6 2nd Sample 8.0 8.0 2nd Sample 7.76 7.68 7.94 12 28 L 491 <0 10 0.6 Treatment 2 0.0 Control CV 5.3 % control or^vusms producotg 3rd brood 100 Sample Type/Duration Grab Comp X LC50= 95% Confidence Limits LCJ0/Acute Toxicity Test (Mortality expressed as %, combining replicates.) Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LCso Collection (Stall) Date: Sample 1 05-01-06 This data package, contains the toxicity test results summarized on this form and includes a Cover Page, AT-1 Form, Laboratory Benchsheets, Statistical Analyses, Chain-of-Custodv Forms and Quality Control Chart's. DWQform AT-1 (3/87) rev. 11/95 •n Facility: Tri test, Inc. Wilson WWTP Laboratory Performing Test: Environmental Testii^/Solutions. Inc Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor: ✓ ____________ Comments: ___________ • LccS-m Project 2521 Samples: 060503.14, 060506.11 Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date K WWTF NPDES#NC002390I |ipe # Comments X MAIL ORIGINAL TO 1 2 3 4 5 6 7 8 9 10 11 12 # Young Produced 19 20 24 19 22 22 17 17 19 22 20 22 % Mortality Avg. Reprod. L L L L L L L L L LAdult (L)ive (D)ead L L Effluent %90.0%0%20.3 Treatment 2 Treatment 2 Control CV TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 10.8%9 10 11 12 # Young Produced 21 20 22 14 22 22 19 22 19 21 20 22 % 3rd Brood L L L L L L L L L L LAdult (L)ive (D)ead L 100% Complete This for Either Test Test Start Date Collection (Start) Date 03-May-06 pH 1 st sample 1st sample 2nd sample Sample 1 5-1-2006 Sample 2 5-4-2006 Control 7.5 7.6 7.6 7.7 7.4 7.9 Sample Type (Duration) Treatment 2 7.4 7.6 7.5 7.8 7.5 7.9 Grab Duration 1st 2nd Sample 1 24hrs.Tox Tox XendendendstartstartSample 2start 24hrs.Dilution Sample Sample ____D.O.1st sample 1st sample 2nd sample 49.0Hardness (mg/L) Control 8.1 8.3 8.4 8.6 8.7 8.4 493Spec. Cond. (pmhos)540 Treatment 2 8.0 8.2 8.58.4 8.4 8.3 0.07Chlorine (mg/L)<0.05 Sample Temp at receipt (°C)1.9 14.7 LC50/Acute Toxicity Test (Mortality expressed as %, combining replicates) Concentration Mortality start/end start/end Method of DeterminationLC50 =Control Probit95% Confidence Limits Moving Average High Cone. %Other pH D.O. Organism Tested DEM Form AT-1 ETT Environmental, Inc. Test invalidated due to 2nd sample receiving temperature of 14.7. 20.3 Control 0% Control North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test CONTROL ORGANISMS Facility: WILSON HOMINY C| Laboratory Performing^Test: 11-May-C6 County: WILSON Comp X 199 —— Signature of Operat^in Responsib^-Charge Signature of Laboratory Supervisor Environmental Sciences Branch Ml*, ui iraici wuamy iw.^. UL.IVH i ifian ocNvivcr vtvttici ixaiciLjii, iwiui \uai oiii id t/upj-iuti PASS FAILrrr Spearman Karber Ceriodaphnia dubia Chronic Test Results __Calculated t=_____.-Q.Q9 Critical Value= 2.508 ! % Reduction= -0% Facility: Wilson NPDES#NC 0023906 Pipe #001 County WilsonLaboratory Performing Test PACE Analytical Services, Inc. MAIL ORIGINAL TO: 12.6 7 8 9 10 11 # Young Produced 18 19 25 23 29 21 27 23 Control Adult (L)ive (D)ead L L L L L L L L L L L L Effluent%90 TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 PASS FAIL # Young Produced 26 21 25 0 20 19 26 22 24 29 13 24 X Adult (L)ive (D)ead L L L D L L L Check OneLLLDL IComplete This For Either Test |1st 1st 2nd ControlPH 7.2 7.3 7.3 7.4 Collection Start Date7.5 7.6 Treatment 7.7 7.2 Sample 17.7 Sample 25\1\06 5\4\06 Samcel Tvce/Duration Grap Comp. Duration Sample 1 X 23 5hr Sample 21st1st2nd X 24hr D.O Control 7.9 7.8 7.5 7.4 7.8 7.7 Hardness (mg/1)43 Treatment 8.7 8.5 Spec. Cond. (umhosl8.1 8.0 7.9 7.8 148 475 497 Chlorine (mg/1)<0.1 <0.1 Sample temo. at receipt .'Cl 3.1 2.8 %%%%%%%% Concentration%%%%%%%%% Mortality LC50=%Method of Determination start/end start/end 95% Confidence Moving Avg,Probit Control % -%Spearman Other pH DO-Organism Tested: Ceriodaphnia dLibia Test Duration (Hours): . E n d E n d E n d 375.9092 375.9091 •iconlrol organisms producing 3rd brood I 100.0 Tabular t % Reduction High Cone. 23.80 Treatment 2 20.30 I 2225 Riverside Drive Asheville, ND 28304 Phone: 828.254.7176 Fax: 828.252.4613 Comments Rank Sum=137.00 1-Tailed Critical 109.00 Avg. Reprca Control LC5O/Acute Toxicity Test fMortahtv expressed as % combining reolicatest % 7,6 s t t a 7,6 s t t a 7.1 s t t a 0.00 Treatment 2 16.7 Cnntrnl CV 15.2 I 4 Note Please Complete This Section also | 26 ^6 - cn Samples Not Aerated Unless Otherwise Noted Environmental Sciences Branch Div. of Water Quality N.C. DENR 1621 Mail Service Center Raleigh, North Carolina 27699-1621 12 1 % Mortality 24 REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc. nehr Chronic Test Results Calculated t Test Start Date: 5\3\06 I North.C.arolinaCenodaphn/a Chronic Pass/Fail Reproduction Toxicity Test CONTROL ORGANISMS 1 2 3 4 5 1 21 Sigudrare of Operator in Responprbfe-Cfiarge I - ■ , SinnatiyLnr I ahnratay Sunfirvisnr , ' / Asheville Certification IDs NC Wastewater 40 NC Drinking Water 37712 SC 99030 FL NELAP E87648 / 9800 Kincey Avenue, Suite 100 / Huntersville, NC 28078 / .Pace Analytic ® P!m^ / "" ™p,1c^toroxicityReP°rt AT-1 Form Chronic Pass/Fail and Acute LC50 / Date 5\16\06 I Charlotte Certification IDs NC Wastewater 12 NC Drinking Water 37706 SC 99006 FL NELAP E87627 6 29 ® Facility: Wilson NPDES#NC 00239C6 Pipe #001 County Wilson Comments MAIL ORIGINAL TO: 0 263 5 6 7 8 9 10 11 12 # Young Produced 20 28 18 23 25 22 23 22 24 25 29 22 Adult (L)ive (D)ead L L L L L L L L L L L L Effluent%90 TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 PASS FAIL tr Young Produced 21 19 21 26 19 24 24 19 27 25 27 25 X Adult (L)ive (D)ead L L L L L L L L Check OneLLLL [Complete This For Either Test1st1st □2nd ControlpH 7.5 7.3 7.5 Collection Start Date7.7 7.8 Treatment Sample 1 Sample 22\6\06 2\9\06 Samoel Tvoe/Duration Grap Comp Duration Sample 1ttt X 23.75hr Sample 21st1st2nd X 25hr D.O Control 8.0 7.9 8.1 8.0 8.1 7.8 43 Treatment 8.6 8.2 8.3 8.0 Soec. Cond. (umhos)8.3 8.1 46:584 Chlorine (mq/l) <C1 <0.1 Samole temo at receiot (C) 2.1 0.7 %%%%%%%% Concentration%%%%%%%% LC50= %Method of Determination start/end start/end 95% Confidence Moving Avg.Probit Control % -%Spearman Other pH D.O. % ____Mortality %control organisms producing 3rd brood | 100.0 High Cone. 12 37706 99006 E87627 Tabular t % Reduction P3CS Anai'/ticai services, Inc. 2225 Riverside Drive Asheville. NC 28804 Phone: 828.254.7176 Fax: 828.252.4618 Hardness (mg/1) 7 SJ f cn o co 5' 0.00 Treatment 2 o.o Cnntrnl CV mH [ft Avg Reprod Control - 23.40 Treatment 2 23.10 LCSO/Acute Toxicity Test IMcrtalitv expressed as % . combinina reclicates) % 7.8 s t a 8.0 E n d 7.6 s t a 7,8 E n d 8.0 s t a ft % Mortality Control 8.2 E n d -fTesl Start Date: 2\8\06 2.508 1.3% Note: Please Complete This Section al so Laboratorv^erforming Test PACE Analytical Services, Inc. Signature of Operator jn RespbnsTBte Charge I ________ , Siogat/re nf LahnrainnLSi inervisor ..-y i/ f y o/ Samples Not Aerated Unless Otherwise Noted Environmental Sciences Branch Div. of Water Quality N.C. DENR 1621 Mail Service Center Raleigh, North Carolina 27699-1621 _________ __Test Duration (Hours): . REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc. nelac Chronic Test Results Calculated t m £ Q) CX tn —< m S =_• (J) ii North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test CONTROL ORGANISMS 12 3 4 PaceAnalyti&l w'M'/v.pacal'a^----- 144 7.7 Organism Tested: Ceriodaphnia dubia Asheville Certification IDs NC Wastewater 40 NC Drinking Water 37712 SC 99030 FL MELAP E87648 race AnaryrrcJi services, inc. 9800 Kincey Avenue, Suite 100 HuntersvW^NC 28078 Phone:^B875.9092 Fax:/U4.875.9091 cEJfeMToxicity ReP°rt ATT Form Chronic Pass/Fail and Acute LC50 Date 2\22\06 - Charlotte Certification IDs NC Wastewater NC Drinkin g Water SC FL NELAP DateEffluent Toxicity Repo 22-Feb-06 County: WILSONNC0023SC6NPDES# Comments MAIL ORIGINAL TO 9 10 11 1278 ■5 63421 Avg. Reprcd.27 26 29 % Mortality252630252824312729# Young Produced L L L LLLLLLLLLAdult (L)ive (D)ead 25.30%90.0%Effluent % Treatment 2Treatment 2 Control CV 8.0%10 127891156423TREATMENT 2 ORGANISMS 1 PASS FAIL27% 3rd Brood3124281922322325261927# Young Produced XL92%L L LLLL LLLLLAdult (L)ive (D)ead Test Start DateComplete This for Either Test 08-Feb-06Collection (Start) Date pH Sample 2 2-9-20062-6-2006Sample 12nd sample1st sample1st sample 7.57.67.77.5 Sample Type (Duration)7.68.3Control 7.6 7.77.8 2nd7.5 Duration 1st7.7 Grab8.2Treatment 2 Tox ToxSample 1 24hrs. X Sample SampleDilution24hrs.Sample 2endstartendstartendstart 49.0D.O.Hardness (mg/L)2nd sample1st sample1st sample 5495108.68.48.58.7 Spec. Cond. (pmhos)8.68.9Control <0.05<0.058.88.68.49.3 Chlorine (mg/L)9.2 8.6Treatment 2 2.11.7Sample Temp, at receipt (°C) LC50/Acute Toxicity Test Concentration start/endstart/endMortality Method of Determination Control Probit High Cone.Moving Average95% Confidence Limits DO.pHOther% Organism Tested DEM Form AT-1 ETT Environmental Tnc. 1.482 2.508 7% 0% Control 27.3 Control Spearman Karber Ceriodaphnia dubia Comp "x” Environmental Sciences Branch miv. vi nuici uuamy II.vs. WL.I1I\ IU4I IVIQII UCI»IUC VsGlllCI ixaiciyii, iwviiii vsai Mima x./ uud-iux i (Mortality expressed as %, combining replicates) LC50 = 194 Chronic Test Results Calculated t= Critical Value= % Reduction= North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test CONTROL ORGANISMS r^prm - Chronic Pass/Fail and Acute LC50^^ Facility: WILSON HOMINY CReW/VVV IF npdes# ncoo23SC6 Laboratory Reforming Test: x X Signature of Operator in Responsible Charge x Signature of Laboratory Supervisor Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC.Date:February 24, 2006so NPDESft NC- 0023906 Pipe 001 County Wilson Comments: ■ I ~ I‘f'. '•") 4 Morth Carolina Ceric.daphnia Chronic Pass/J'aii Reproduction Toxicity Test Percent Pass Fail X Test Start Date: 02-08-06 Sample 2 02-09-06 Duration 23 75-h 25-h 32. 34. 33 to Control Higli Cone. Ceriodaphnia dubiaOrganism Tested:Duration:7-days pH (S.U.)DO (nig/L) I Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center Raleigh, NC 27699-1621 D.O. (mg/L) Control Treatment 2 Method of Determination _ Trimmed Spearman Karbcr Probit Other: Sample 1 Sample 2 Alkalinity (mg CaCOj/L) Hardness (mg CaCOj.L) Conductivity (jimhos/cm) Total Residual Chlorine (mg'L) Sample Temp, at Receipt ("C) Chronic Test Results Calculated t: Tabular t: % Reduction: Concentration (%) Mortality (%) I pH (S.U.) Control Treatment 2 PO Box 7565 Asheville, NC 23S02 ■o W Treatment 2 33.6 -o J 3 CO c*i i W I I I 3 Average Reproduction Control 31.2 Effluent Percentage! 90% Treatment 2 Organisms Number of Young Produced Adult Survival: (L)ive, (D)ead Control Organisms__________ Number of Young Produced Adult Survival: (L)ive, (D)ead 7.57 7,6 8.0 7.64 8.10 7,7 7.5 3 36 L 4 34 L 5 31 L 7.53 7.96 7.5 7/7 6 31 L 7 31 L 8 30 L 8 33 L 10 11 33 L 32 L 29 L Project: Samples: Morality Control 0.0 2370 060208.16. 060211.22 1 33 L 5 33 L 7 36 L 9 34 L 10 11 34 T" ___ 492 <0.10 0.8 -2.342 2.508 -7.8 1 st Sample 7.8 8.3 3 36 L 4 38 L 2nd Sample 7,64 8.00 581 <0.10 0.8 1st Sample 7,69 Tt? 1st Sample 7.8 8.0 2 33 L 2 31 L 2nd Sample 7,8 7.8 9 27 L 12 32 L 12 32 L 1 26 L Treatment 2 0.0 Control CV 44, 44. 40 Itc. 157, 157 1 st Sample T50 T02 9.1 % control cngBirnis producing 3rd Hood 100 6 32 L Sample Type/Duration Grab Comp. x LC50 = 95% Confidence Limits LCS0/Acute Toxicity Test (Mortality expressed as %, combining replicates.) ■h Collection (Start) Date: Sample 1 024)6-06 This data package contains the toxicity test results summarized on this form and includes a Cover Page. AT-1 Form. Lahoratorv Benchsheets, Statistical Analyses. Chain-of-Custodv Forms and Quality Control Charts. DWQform AT-1 (3/87) rev. 11/95 Ij^ivironmental Testing Solutions, Inc. Phone: (828) 350-9364 Ty Fax: (828) 350-9368 E-mail: JimSumner@aol.coni Facility: Tritest. Inc. Wilson WWTP Laboratory Performing Test: Environmental Testing Solutions. Inc. Signature of Operator in Responsible Charge: zZi Signature of Laboratory Supervisor: