HomeMy WebLinkAboutNC0023906_Lab analysis_20061231Date 17-Nov-06
County: WILSONNPDES#NC002390('ipe #
Comments
MAIL ORIGINAL TO
1 2 3 4 5 6 7 8 9 10 11 12
H Young Produced 30 27 25 21 30 29 29 29 24 26 27 22 % Mortality Avg. Reprod.
Adult (L)ive (D)ead L L L L L L L L L L L L
Effluent %90.0%0%24.4
Treatment 2 Treatment 2
Control CV
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 11.5%10 11 12
# Young Produced 20 25 27 25 21 26 29 24 25 24 20 27 % 3rd Brood
Adult (L)ive (D)ead L L L L L L L L L L L L 100%
Complete This for Either Test Test Start Date
Collection (Start) Date 11-8-06pH1st sample 1st sample 2nd sample Sample 1 11-6-2006 Sample 2 11-9-2006
Control 7.3 7.5 7.7 7.7 7.6 7.6 Sample Type (Duration)
Treatment 2 7.5 7.7 7.3 8.1 7.8 7.6 Grab Duration 1st 2nd
Sample 1 24hrs.Tox Tox
start end start end start end XSample 2 "24hrs.Dilution Sample Sample
D.O.1st sample 1st sample 2nd sample Hardness (mg/L)46.0
Control 8.8 8.7 8.7 8.5 8.4 8.3 Spec. Cond. (pmhos)552 485
Treatment 2 8.7 8.7 8.7 8.5 8.4 7.6 Chlorine (mg/L)0.08 <0.05
Sample Temp, at receipt (°C)2.1 2.7
LC50/Acute Toxicity Test
start/end start/endLC50 =
Control
95% Confidence Limits ProbitMoving Average High Cone.
%Spearman Karber pH D.O.
Organism Tested J
DEM Form AT-1
ETT Environmental, Inc.
1.797
2.508
8%
0%
Control
26.6
Control
|Other
Ceriodaphnia dubia
Comp
T"
Chronic Test Results
Calculated t=
Critical Value=
% Reduction=
Environmental Sciences Branch
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North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
PASS FAIL
TF
(Mortality expressed as %, combining replicates)
~lll III FR
Method of Determination
183 ,
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
.-acility: WILSON WWTF
Laboratory Performing Test:
Signature of Operator in Responsible Charge
2S____ _______
Signature of Laboratory Supervisor
Inc.En^onmental Testing Solutio,
November 24, 2006Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC5o Date:
County: WilsonNPDES#: NC- 00'23906 Pipe #: 001
Comments: g/c-/00(55^
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent
LLLLL
FailPass
XLLLLLLLLL
11-09-06Sample 2
Ehirnion
W-)i
24-h
’? 33
____
Control
Higli Cone.
DO (nig/1)pH (S.U.)7-daysDuration:Ceriodaphnia dubiaOrganism Tested:
PO Box 7565
Asheville, NC 28802
D.O. (mg/L)
Control
Treatment 2 Sample 1
Sample 2
Alkalinity (mg CaCO3/L)
Hardness (nig CaCOj/L)
Conductivity (jimhos/cm)
Total'Residual Chlorine Ung/L)
Sample Temp. atReceipt (°C)
Phone: (828) 350-9364
Fax: (828) 350-9368
E-mail: JimSumner@ aol.com
Chronic Test Results
Calculated t;
Tabular t:
% Reduction:
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
Concentration (%)
Mortality (%)
3
a
pH (S.U.)
Control
Treatment 2
Treatment 2
35.8
I
I
I
3
55 3I
J
3
3
2
31
L
8.3
8.1
31
L
Project:
Samples:
2857
061108.04, 061111.04
Control Organisms_________
Number of Young Produced
Adult Survival: (L)ive, (D)ead
8.1
8.4
7.8
8.4
9
32
L
10 11
30
L
12
30
L
Mortality
Control
0.0
Average
Reprodnction
Control
30.0
461
<0.10
0.4
1
30
L
1st Sample
7.2917.66'
7.80 8.09
1st Sample
8.2
8.4
1st Sample
7.6
8.4
1st Sample
7.55 7.39
8.03 7.85
-9.324
2.508
-19.4
553
<0.10
0.7
Treatment 2
0.0
Control C V
4.9
7. control orjwttsms
prottarinf 3rd brood
100
3 4 5 6 7 8
28 31 28 29 32 28
T
LCjo/Acute Toxicity Test
(Mortality expressed as %, combining replicates.)
Method of Determination
Trimmed Spearman Karber
Probit
Other:
LC50 =
95% Confidence Limits
to
12 3
37 | 37 | 35
L
45'678
36 | 37 | 33 | 38 | 35
L
Sample Type/Duration
Ck»l> Comp.
x
44. 42
.64 149.
153
_____
2nd Sample
7.75 17.36
7.92" 7.79
2nd Sample
8.2
8.4
Facility: Tritest. Inc.
Wilson WWTP
Laboratory Performing Test: Environmental Testing, Solutions, Inc.
Signature of Operator in Responsible Charge: -----
Signature of Laboratory Supervisor: --------------
9 IQ 11 12
35 | 35 | 38 | 34
T
11
Collection (Start) Date:
Sample 1 11-06-06
This data package contains the toxicity test results summarized on this form and includes a Cover Page,
AT-I Form, Laboratory Benchsheets, Statistical Analyses, Chain-of-Custody Forms and Quality Control Charts.
DWQ form AT-1 (3/87) rev. 11/95
Effluent Percentage 90%
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
Test Start Date: 11-08-06
Date 22-Aug-06
County: WILSONNPDES#NC0023906 ie #
Comments
MAIL ORIGINAL TO
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
725 6 8 9 10 11 12134
Avg. Reprod.23 22 27 22 31 28 23 % Mortality# Young Produced 26 21 27 28 25
LLLLLL L L L L LLAdult (L)ive (D)ead
19.917%90.0%Effluent %
Treatment 2Treatment 2
Control CV
12.2%5 6 7 8 9 10 11 12TREATMENT 2 ORGANISMS 1 2 3 4
25 % 3rd Brood21918252122 025282421# Young Produced
D 100%D L L L L LLL L L LAdult (L)ive (D)ead
Test Start DateComplete This for Either Test
Collection (Start) Date 09-Aug-06
pH Sample 2 8-10-20062nd sample Sample 1 8-7-20061st sample1st sample
7.5 7.47.8 7.5Control7.9 8.0
Sample Type (Duration)
7.7 7.67.8 7.77.8 7.9Treatment 2 1st 2ndGrabDuration
Tox ToxSample 1 24hrs.
X Sample SampleSample 2 24hrs.Dilutionendendendstartstartstart
D.O.2nd sample 46.1Hardness (mg/L)1st sample 1st sample
562 5968.0 8.48.2 8.5 8.18.1Control Spec. Cond. (pmhos)
<0.05<0.057.9 8.48.7 8.08.6Treatment 2 8.9 Chlorine (mg/L)
2.1 0.8Sample Temp, at receipt (°C)- V-v -
LCSO/Acute Toxicity Test
(Mortality expressed as %. combining replicates)
Concentration
start/endstart/endMortality
Method of DeterminationLC50 =Control
Probit High Cone.Moving Average95% Confidence Limits
D.O.pHOther%
JOrganism Tested
DEM Form AT-1
ETT Environmental, Inc.
Spearman Karber
Ceriodaphnia dubia
113
109
21%
0%
Control
25.3
Control
Comp
T"
Environmental Sciences Branch
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Chronic Test Results
Rank sum=
Critical Value=
% Reduction=
PASS FAIL
ITT
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
ucility: WILSON HOMINY CF^KWWTF
Labor^jory Performing Test:
x zA..
Signature of Operator ResponsfoJ<pharge
x___________
Signature of Laboratory Supervisor
August 21, 2006Effluent Toxicity Report Form - Chronic Pass,Tail and Acute LC50 Date:
County: WilsonPipe #: 001NPDES #: NC- 0023906
Comments:
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent
90%
FailPass
X
LL
Test Start Date: 08-09-06
7.23
08-10-06Sample 2
DusaUcn
22.75-h
23 75-h
35. 33
Control
High Cone.
DO (mg/L)pH (S.U.)7-daysDuration:Ceriodaphnia dubiaOrganism Tested:
1
34
L
3
34
L
2
3?
T
Effluent Percentage
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
D.O. (mg/L)
Control
Treatment 2 Sample 1
Sample 2
Alkalinity (mg CaCOyL)
Hardness (mgCaCO/L)
Conductivity (itmhos/cm)
Total Residual Chlorine (mg/L)
Sample Temp, at Receipt (°C)
Chronic Test Results
Calculated t:
Tabular t:
% Reduction:
I I
I
Concentration (%)
Mortality (%)
pH (S.U.)
Control
Treatment 2
LC50 =
95% Confidence Limits
to
3
Treatment 2
33.8
I
'TO Box 7565
Asheville, NC 28802
I.
rl
I
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQZ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
I
I
3
3
Contrui Organisms__________
Number of Young Produced
Adult Survival: (L)ive, (D)ead
8.1
8.3
7.8
8.2
2_9_
31
L
11
31
L
11
34
L
12
32
L
Project:
Samples:
2690
060809.04, 060812.15
Average
Reproduction
Control
30.230
T-
4
30
L
5
3?
L
6
30
L
7
33
L
9
37
T
w
30
L
10
30
L
12
33
L
Mortality
Control
0.0
530
<0.10
0.6
1st Sample
7.341 7.24'
7.68 7.68
7
32
L
45. 43
>». 1’4.
156
-4.615
2.508
-12.2
536
<0.10
0.7
1st Sample
8.0
8.2
1st Sample
7,3617.15"
7.66 7.67
1st Sample
8.1
”83
8
28
L
8
32
L
Treatment 2
0.0
Control CV
4.4
% control orgsasnis
producing 5rd brood
100
1
28
L
7.9
8.2
Sample Type/Duration
Grab Comp.
x”
X
*(82^ 350-9368
E-mail: JimSiminer@aol.com
Method of Determination
__Trimmed Spearman Karber
Probit
Other:
LC50ZAcute Toxicity Test
(Mortality expressed as %, combining replicates.)
4 5 6
36 | 30 | 37
L
lolutions, Inc.
2nd Sample
7.04
7.56 7.58
2nd Sample
8.0
8.1 ii
Collection (Start) Date:
Sample 1 08-07-06
This data package contains the toxicity test results summarized on this form and includesa Cover Page
AT-1 Form, Laboratory Benchsheets. Statistical Analyses. Chain-ofC us tody Forms and Quahty Control Charts_
DWQfonn AT-1 (3/B7) rev. 11/95
Facility: Tritest. Inc.
Wilson WWTP
Laboratory Performing Test: Environmental Testing^
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor:
3
29
L
Date:May 19, 2006
NPDES #: NC- 0023906 Pipe #: 001 County: Wilson
th Carolina Ccriodaphriia Chronic Pass/Fail Reproduction To.viJty Test Pt-lCcHl
FailPass
X
Test Start Date: 05-03-06
7.80 8.02
Sample 2 05-04-06
Du radon
23.5-h
24 h
.<5. J3
3 to
Control
High Cone.
Ceriodaphnia dubia Duration:7-days pH (S.U.)DC) (mg/L)Organism Tested:
PO Box 7565
Asheville, NC 28302
Environmental Testing Solutions, Inc.
Phone: (828) 350-9364
Fax: (828) 350-9368
E-mail: JimSumner@aol.com
Sample 1
Sample 2
Chronic Test Results
Calculated t:
Tabular t:
% Reduction:
1
Concentration (%)
Mortality (%)
D.O. (mg/L)
Control
Treatment 2
Alkalinity (mg CaCOj/L)
Hardness (mg CaCO3/Li
Conductivity (pmlios/cut)
Total Residual Chlorine (mg/L)
Sample Temp, al Receipt (°C)
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
pH (S.U.)
Control
Treatment 2
I
1
I
Treatment 2
31.4
I
f
•o
(S 33
I
I
Average
Reproduction
Control
28.8
Control Organisms_________
Number of Young Produced
Adult Survival: (L)ive. (D)ead
7.8
8.2
2
33
L
2
31
L
7.79
7.94
7.8
7.8
3
35
L
3
28
L
4
31
L
4
30
L
7.8
7.8
6
28
L
7
29
L
8
34
L
10
32
T
u
28
L
Mortality
Control
0.0
Effluent Percentage 90%
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive. (D)ead
1
27
L
1
29
L
5
26
L
5
31
L
6
35
L
8
30
L
9
30
L
9
31
L
10
27
L
11
32
L
-2.959
2.508
-9.0
1st Sample
7.6
7.5
1st Sample
7,78
7.78
7
29
L
12
29
L
41.41
155. 157.
153 550
<0.10
0.5
1st Sample
7.83 7.68
7.64
Method of Determination
ITrimmed Spearman Karber
Probit
LjOther: ________________
1st Sample
7.7
7.6
2nd Sample
8.0
8.0
2nd Sample
7.76 7.68
7.94
12
28
L
491
<0 10
0.6
Treatment 2
0.0
Control CV
5.3
% control or^vusms
producotg 3rd brood
100
Sample Type/Duration
Grab Comp
X
LC50=
95% Confidence Limits
LCJ0/Acute Toxicity Test
(Mortality expressed as %, combining replicates.)
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LCso
Collection (Stall) Date:
Sample 1 05-01-06
This data package, contains the toxicity test results summarized on this form and includes a Cover Page,
AT-1 Form, Laboratory Benchsheets, Statistical Analyses, Chain-of-Custodv Forms and Quality Control Chart's.
DWQform AT-1 (3/87) rev. 11/95
•n
Facility: Tri test, Inc.
Wilson WWTP
Laboratory Performing Test: Environmental Testii^/Solutions. Inc
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: ✓ ____________
Comments: ___________
• LccS-m
Project 2521
Samples: 060503.14, 060506.11
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date
K WWTF NPDES#NC002390I |ipe #
Comments
X
MAIL ORIGINAL TO
1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced 19 20 24 19 22 22 17 17 19 22 20 22 % Mortality Avg. Reprod.
L L L L L L L L L LAdult (L)ive (D)ead L L
Effluent %90.0%0%20.3
Treatment 2 Treatment 2
Control CV
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 10.8%9 10 11 12
# Young Produced 21 20 22 14 22 22 19 22 19 21 20 22 % 3rd Brood
L L L L L L L L L L LAdult (L)ive (D)ead L 100%
Complete This for Either Test Test Start Date
Collection (Start) Date 03-May-06
pH 1 st sample 1st sample 2nd sample Sample 1 5-1-2006 Sample 2 5-4-2006
Control 7.5 7.6 7.6 7.7 7.4 7.9 Sample Type (Duration)
Treatment 2 7.4 7.6 7.5 7.8 7.5 7.9 Grab Duration 1st 2nd
Sample 1 24hrs.Tox Tox
XendendendstartstartSample 2start 24hrs.Dilution Sample Sample
____D.O.1st sample 1st sample 2nd sample 49.0Hardness (mg/L)
Control 8.1 8.3 8.4 8.6 8.7 8.4 493Spec. Cond. (pmhos)540
Treatment 2 8.0 8.2 8.58.4 8.4 8.3 0.07Chlorine (mg/L)<0.05
Sample Temp at receipt (°C)1.9 14.7
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
Concentration
Mortality start/end start/end
Method of DeterminationLC50 =Control
Probit95% Confidence Limits Moving Average High Cone.
%Other pH D.O.
Organism Tested
DEM Form AT-1
ETT Environmental, Inc.
Test invalidated due to 2nd sample
receiving temperature of 14.7.
20.3
Control
0%
Control
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
Facility: WILSON HOMINY C|
Laboratory Performing^Test:
11-May-C6
County: WILSON
Comp
X
199
——
Signature of Operat^in Responsib^-Charge
Signature of Laboratory Supervisor
Environmental Sciences Branch
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iw.^. UL.IVH
i ifian ocNvivcr vtvttici
ixaiciLjii, iwiui \uai oiii id t/upj-iuti
PASS FAILrrr
Spearman Karber
Ceriodaphnia dubia
Chronic Test Results
__Calculated t=_____.-Q.Q9
Critical Value= 2.508
! % Reduction= -0%
Facility: Wilson NPDES#NC 0023906 Pipe #001
County WilsonLaboratory Performing Test PACE Analytical Services, Inc.
MAIL ORIGINAL TO:
12.6
7 8 9 10 11
# Young Produced 18 19 25 23 29 21 27 23 Control
Adult (L)ive (D)ead L L L L L L L L L L L L
Effluent%90
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 PASS FAIL
# Young Produced 26 21 25 0 20 19 26 22 24 29 13 24 X
Adult (L)ive (D)ead L L L D L L L Check OneLLLDL
IComplete This For Either Test |1st 1st 2nd
ControlPH 7.2 7.3 7.3 7.4 Collection Start Date7.5 7.6
Treatment 7.7 7.2 Sample 17.7 Sample 25\1\06 5\4\06
Samcel Tvce/Duration
Grap Comp. Duration
Sample 1 X 23 5hr
Sample 21st1st2nd X 24hr
D.O Control 7.9 7.8 7.5 7.4 7.8 7.7 Hardness (mg/1)43
Treatment 8.7 8.5 Spec. Cond. (umhosl8.1 8.0 7.9 7.8 148 475 497
Chlorine (mg/1)<0.1 <0.1
Sample temo. at receipt .'Cl
3.1 2.8
%%%%%%%%
Concentration%%%%%%%%%
Mortality
LC50=%Method of Determination start/end start/end
95% Confidence Moving Avg,Probit Control
% -%Spearman Other
pH DO-Organism Tested: Ceriodaphnia dLibia Test Duration (Hours): .
E
n
d
E
n
d
E
n
d
375.9092
375.9091
•iconlrol organisms
producing 3rd brood
I 100.0
Tabular t
% Reduction
High
Cone.
23.80
Treatment 2
20.30
I
2225 Riverside Drive
Asheville, ND 28304
Phone: 828.254.7176
Fax: 828.252.4613
Comments
Rank Sum=137.00
1-Tailed Critical 109.00
Avg. Reprca
Control
LC5O/Acute Toxicity Test
fMortahtv expressed as % combining reolicatest
%
7,6
s t
t
a
7,6
s t
t
a
7.1
s t
t
a
0.00
Treatment 2
16.7
Cnntrnl CV
15.2 I
4
Note Please
Complete This
Section also
|
26
^6
- cn
Samples Not Aerated Unless Otherwise Noted
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
12
1 % Mortality
24
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
nehr
Chronic Test Results
Calculated t
Test Start Date:
5\3\06
I
North.C.arolinaCenodaphn/a Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS 1 2 3 4 5
1
21
Sigudrare of Operator in Responprbfe-Cfiarge
I - ■ ,
SinnatiyLnr I ahnratay Sunfirvisnr , ' /
Asheville Certification IDs
NC Wastewater 40
NC Drinking Water 37712
SC 99030
FL NELAP E87648
/ 9800 Kincey Avenue, Suite 100
/ Huntersville, NC 28078
/ .Pace Analytic ® P!m^
/ "" ™p,1c^toroxicityReP°rt AT-1 Form Chronic Pass/Fail and Acute LC50
/ Date 5\16\06
I
Charlotte Certification IDs
NC Wastewater 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627
6
29
®
Facility: Wilson NPDES#NC 00239C6 Pipe #001
County Wilson
Comments
MAIL ORIGINAL TO:
0 263
5 6 7 8 9 10 11 12
# Young Produced 20 28 18 23 25 22 23 22 24 25 29 22
Adult (L)ive (D)ead L L L L L L L L L L L L
Effluent%90
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 PASS FAIL
tr Young Produced 21 19 21 26 19 24 24 19 27 25 27 25 X
Adult (L)ive (D)ead L L L L L L L L Check OneLLLL
[Complete This For Either Test1st1st □2nd
ControlpH 7.5 7.3 7.5 Collection Start Date7.7 7.8
Treatment Sample 1 Sample 22\6\06 2\9\06
Samoel Tvoe/Duration
Grap Comp Duration
Sample 1ttt X 23.75hr
Sample 21st1st2nd X 25hr
D.O Control 8.0 7.9 8.1 8.0 8.1 7.8 43
Treatment 8.6 8.2 8.3 8.0 Soec. Cond. (umhos)8.3 8.1 46:584
Chlorine (mq/l)
<C1 <0.1
Samole temo at receiot (C)
2.1 0.7
%%%%%%%%
Concentration%%%%%%%%
LC50= %Method of Determination start/end start/end
95% Confidence Moving Avg.Probit Control
% -%Spearman Other
pH D.O.
%
____Mortality
%control organisms
producing 3rd brood
| 100.0
High
Cone.
12
37706
99006
E87627
Tabular t
% Reduction
P3CS Anai'/ticai services, Inc.
2225 Riverside Drive
Asheville. NC 28804
Phone: 828.254.7176
Fax: 828.252.4618
Hardness (mg/1)
7
SJ
f
cn
o
co
5'
0.00
Treatment 2
o.o
Cnntrnl CV
mH
[ft Avg Reprod
Control
- 23.40
Treatment 2
23.10
LCSO/Acute Toxicity Test
IMcrtalitv expressed as % . combinina reclicates)
%
7.8
s
t
a
8.0
E
n
d
7.6
s
t
a
7,8
E
n
d
8.0
s
t
a
ft % Mortality
Control
8.2
E
n
d
-fTesl Start Date:
2\8\06
2.508
1.3%
Note: Please
Complete This
Section al so
Laboratorv^erforming Test PACE Analytical Services, Inc.
Signature of Operator jn RespbnsTBte Charge
I ________
, Siogat/re nf LahnrainnLSi inervisor ..-y
i/ f y o/
Samples Not Aerated Unless Otherwise Noted
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
_________ __Test Duration (Hours): .
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
nelac
Chronic Test Results
Calculated t
m £
Q) CX
tn —<
m S
=_• (J)
ii
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS 12 3 4
PaceAnalyti&l
w'M'/v.pacal'a^-----
144
7.7
Organism Tested: Ceriodaphnia dubia
Asheville Certification IDs
NC Wastewater 40
NC Drinking Water 37712
SC 99030
FL MELAP E87648
race AnaryrrcJi services, inc.
9800 Kincey Avenue, Suite 100
HuntersvW^NC 28078
Phone:^B875.9092
Fax:/U4.875.9091
cEJfeMToxicity ReP°rt ATT Form Chronic Pass/Fail and Acute LC50
Date 2\22\06 -
Charlotte Certification IDs
NC Wastewater
NC Drinkin g Water
SC
FL NELAP
DateEffluent Toxicity Repo 22-Feb-06
County: WILSONNC0023SC6NPDES#
Comments
MAIL ORIGINAL TO
9 10 11 1278 ■5 63421
Avg. Reprcd.27 26 29 % Mortality252630252824312729# Young Produced
L L L LLLLLLLLLAdult (L)ive (D)ead
25.30%90.0%Effluent %
Treatment 2Treatment 2
Control CV
8.0%10 127891156423TREATMENT 2 ORGANISMS 1
PASS FAIL27% 3rd Brood3124281922322325261927# Young Produced
XL92%L L LLLL LLLLLAdult (L)ive (D)ead
Test Start DateComplete This for Either Test
08-Feb-06Collection (Start) Date
pH Sample 2 2-9-20062-6-2006Sample 12nd sample1st sample1st sample
7.57.67.77.5 Sample Type (Duration)7.68.3Control
7.6 7.77.8 2nd7.5 Duration 1st7.7 Grab8.2Treatment 2
Tox ToxSample 1 24hrs.
X Sample SampleDilution24hrs.Sample 2endstartendstartendstart
49.0D.O.Hardness (mg/L)2nd sample1st sample1st sample
5495108.68.48.58.7 Spec. Cond. (pmhos)8.68.9Control
<0.05<0.058.88.68.49.3 Chlorine (mg/L)9.2 8.6Treatment 2
2.11.7Sample Temp, at receipt (°C)
LC50/Acute Toxicity Test
Concentration
start/endstart/endMortality
Method of Determination Control
Probit High Cone.Moving Average95% Confidence Limits
DO.pHOther%
Organism Tested
DEM Form AT-1
ETT Environmental Tnc.
1.482
2.508
7%
0%
Control
27.3
Control
Spearman Karber
Ceriodaphnia dubia
Comp
"x”
Environmental Sciences Branch
miv. vi nuici uuamy
II.vs. WL.I1I\
IU4I IVIQII UCI»IUC VsGlllCI
ixaiciyii, iwviiii vsai Mima x./ uud-iux i
(Mortality expressed as %, combining replicates)
LC50 =
194
Chronic Test Results
Calculated t=
Critical Value=
% Reduction=
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
r^prm - Chronic Pass/Fail and Acute LC50^^
Facility: WILSON HOMINY CReW/VVV IF npdes# ncoo23SC6
Laboratory Reforming Test:
x X
Signature of Operator in Responsible Charge
x
Signature of Laboratory Supervisor
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC.Date:February 24, 2006so
NPDESft NC- 0023906 Pipe 001 County Wilson
Comments: ■ I ~ I‘f'. '•") 4
Morth Carolina Ceric.daphnia Chronic Pass/J'aii Reproduction Toxicity Test Percent
Pass Fail
X
Test Start Date: 02-08-06
Sample 2 02-09-06
Duration
23 75-h
25-h
32. 34. 33
to
Control
Higli Cone.
Ceriodaphnia dubiaOrganism Tested:Duration:7-days pH (S.U.)DO (nig/L)
I
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
D.O. (mg/L)
Control
Treatment 2
Method of Determination
_ Trimmed Spearman Karbcr
Probit
Other:
Sample 1
Sample 2
Alkalinity (mg CaCOj/L)
Hardness (mg CaCOj.L)
Conductivity (jimhos/cm)
Total Residual Chlorine (mg'L)
Sample Temp, at Receipt ("C)
Chronic Test Results
Calculated t:
Tabular t:
% Reduction:
Concentration (%)
Mortality (%)
I
pH (S.U.)
Control
Treatment 2
PO Box 7565
Asheville, NC 23S02
■o
W
Treatment 2
33.6
-o
J
3
CO
c*i
i
W
I I
I
3
Average
Reproduction
Control
31.2
Effluent Percentage! 90%
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
Control Organisms__________
Number of Young Produced
Adult Survival: (L)ive, (D)ead
7.57
7,6
8.0
7.64
8.10
7,7
7.5
3
36
L
4
34
L
5
31
L
7.53
7.96
7.5
7/7
6
31
L
7
31
L
8
30
L
8
33
L
10 11
33
L
32
L
29
L
Project:
Samples:
Morality
Control
0.0
2370
060208.16. 060211.22
1
33
L
5
33
L
7
36
L
9
34
L
10 11
34
T"
___
492
<0.10
0.8
-2.342
2.508
-7.8
1 st Sample
7.8
8.3
3
36
L
4
38
L
2nd Sample
7,64
8.00
581
<0.10
0.8
1st Sample
7,69
Tt?
1st Sample
7.8
8.0
2
33
L
2
31
L
2nd Sample
7,8
7.8
9
27
L
12
32
L
12
32
L
1
26
L Treatment 2
0.0
Control CV
44, 44. 40
Itc. 157,
157
1 st Sample
T50
T02
9.1
% control cngBirnis
producing 3rd Hood
100
6
32
L
Sample Type/Duration
Grab Comp.
x
LC50 =
95% Confidence Limits
LCS0/Acute Toxicity Test
(Mortality expressed as %, combining replicates.)
■h
Collection (Start) Date:
Sample 1 024)6-06
This data package contains the toxicity test results summarized on this form and includes a Cover Page.
AT-1 Form. Lahoratorv Benchsheets, Statistical Analyses. Chain-of-Custodv Forms and Quality Control Charts.
DWQform AT-1 (3/87) rev. 11/95
Ij^ivironmental Testing Solutions, Inc.
Phone: (828) 350-9364
Ty Fax: (828) 350-9368
E-mail: JimSumner@aol.coni
Facility: Tritest. Inc.
Wilson WWTP
Laboratory Performing Test: Environmental Testing Solutions. Inc.
Signature of Operator in Responsible Charge: zZi
Signature of Laboratory Supervisor: