NC0023906_Lab analysis_20051231

Facility: Wilson NPDEStfNC 0023906 Pipe #001
County WilsonInc.
Comments
MAIL ORIGINAL TO:-0.909
6 7 8 9 10 11 12
# Young Produced 21 22 25 23 25 19 17 21 20 23 16 24
Adult (L)ive (D)ead L L L L L L L L L L L L
Effluent%90
TREATMENT 2 ORGANISMS 1 2 3 5 6 7 8 9 10 11 12 PASS FAIL
# Young Produced 19 23 26 17 26 25 22 24 23 20 17 X
Adult (L)ive (D)ead L L L L L L L Check OneLLLLL
[Complete This For Either Te~sF1st ]1st 2nd
ControlPH 7.2 7.3 7.3 7.5 Collection Start Date7.2 7.3
Treatment Sample 1 Sample 211\7\05 11\10\05
Samoel Tvoe/Duration
Grab Comp Duration
Sample 1ttt X 24hr
1st Sample 21st2nd X 24hr
D.O Control 7.6 7.6 7.6 7.4 8.2 8.0 Hardness (mg/l)43
Treatment 8.0 7.8 7.9 7.8 Spec. Cond (umhos)8.0 7.7 143 450 542
Chlorine (mp/l)
<0.1 <0.1
Sample temo. at receiot (C)
2.6
%%%%%%%%
Concentration%%%%%%%%%
Mortality
LC50=%Method of Determination start/end start/end
95% Confidence Moving Avg.Probit Control
% -%Spearman Other
pH D.O.
%control organisms
producing 3rd brood
| 100.0
Tabular!
% Reduction
Face Analytical Services, Inc.
2225 Riverside Drive
Asheville. NC 23804
Phone: [phone]
Fax: [phone]
High
Cone
I
2.508
0
LC50/Acbte Toxicity Test
iMortalitv expressed as % , combining replicates)
%
% Mortality
Control
0.00
Treatment 2
P.O
Cnntrnl CIV
I isl-]
21.30
Treatment 2
22.60
7,7
s
t
a
7,6
E
n
d
7,8
S
t
a
|J[ Avg. Reprod.
Control
8.0
E
n
d
7.7
s
t
a
7.7
E
n
d
2.0
zz
Note: Please
Complete This
Section also
Test Duration (Hours): .
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
nphr
Samples Not Aerated Unless Otherwise Noted
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Chronic Test Results
Calculated t
•1 Test Start Date: f
11\9\05
North Carolina Ceriodaphnia Chronc Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS 1 2 3 4 5
m S’
LU Q.
is
Organism Tested: Ceriodaphnia dubia
Asheville Certification IDs
NC Wastewater 40
NC Drinking Water 37712
SC 99030
FL NELAP E87648
LaboratdTy Performing Test PACE Analytical Services.
, Signature of Operator irfKespbnsible Charge
[ Sicpaii ire nl Labnp^tnrv Si innrvisry-^r
4
29 J
I /Q PT~ F Pace Ana,ytlcal Services. Inc.
J 9800 Kincey Avenue, Suite 100
S A HuntersviUgHC 28078
/^-Pace Analytical Fax: [phone]^1
/ Report AT-1 Form Chronic Pass/Fail and Acute LC50
I Date 11/9/05
i
Charlotte Certification IDs
NC Wastewater 12
NC Drinking Water 37706
SC 99006
FL NELAP E87627
Date:November 28, 2005
NPDES NC- 0023906 Pipe#: 001 County Wilson
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent
9
Pass Fail
X
Test Start Date:11-09-05
8.06
Sample 2 11-10-05
Duration
24-h
24.h
34
Control
High Cone.
Organism Tested:Ceriodaphnia duhia Duration:7-days pH (S.U.)DO (mg-'L)
DWQform AT-1 (3/87) rev. 11/95
10
5
d"
11
31
L
12
25
L
Concentration (%)
Mortality (%)
D.O. (mg/L)
Control
Treatment 2
PO Box 7565
Asheville, NC 28802
Method of Determination
Trimmed Spearman Karber
Probit
Other:
Sample i I
Sample 2
Alkalinity (mg CaCOyl)
Hardness (mg CaCO3/L)
Con due ti vi tj' (pmhos'cni)
1 otal Residual Chlorine (nigT)
Sample Temp, at Receipt ("C)
Mail Original To: North Carolina Department of Environment and Natural Resources
DVVQ/ Environmental Sciences Branch
1621 Mail Sendee Center
Raleigh. NC 27699-1621
pH (S.U.)
Control
Treatment 2
•c
5
Chronic Test Results
Calculated t: NA
Tabular t:
% Reduction:
r:
& i
-o
t
5co
I nd
£
1
I
Effluent Percentage 90%
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
Morality
Control
0.0
Control Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
7,5
77
3
28
L
5
31
L
7.8
To
6
30
L
7
26
77
8
32
L
7.72
T04
1
31
L
2
33
77
5
30
L
7.63
Z98
11
30
L
485
<0.10
0.5
1st Sample
7.68 7.75
7.89
3
31
L
2nd Sample
7.69
8.03
6
25
L
7
32
77
12
26
L
558
<0.10
0.8
1 st Sample
7.66
777
1
26
L
8
29
L
9
31
L
10
28
L
NA
-1.8
Average
Reproduction
Control
27.6
Treatment 2
28.1
40
IS-4. 154.
!5»
4
25
L Treatment 2
8,3
Control CV
29
77
9
28
L
7.6
77
7,2
% control ofganunik
producing 3rd brood
100
4
26
L
Sample Type/Puration
Grub Conip.
x~
X
LCjo/Acute Toxicity Test
(Mortality expressed as %, combining replicates.)
LC50 =
95% Confidence Limits
to
1st Sample
7.6
0
1st Sample
7.8
2nd Sample
7.8
"77
^pvironmental Testing Sol^jons, Inc.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Collection (Start) Date:
Sample 1 11-07-05
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC^0
Comments:
Rs ° 185 50, 1-Tailed Critical = 109 00
Project: 2201_________________
Samples: 051109.02, 051112.08
Facility Tritest, Inc.
Wilson WWTP
Laboratory Perfonning Test: Environmental Testing-Solutions, Inc
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervison
Date
1

2 3 4 5 6 7 8 9 10 11 12
ft Young Produced 27 21 28 24 21 19 28 22 26 27 23 27 |°/o Mortality Avg Reprod.Adult (L)ive (D)ead L L L L I i-L I.L L L L
Effluent %90.0%
25%10.6
Treatment 2 Treatment 2
Control CVTREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 12.9%it- Young Produced 16 8 11 12 0 21 19 0 6 13 0 21 FAILAdult (L)ive (D)ead L L L L D L L L D L D L 100%X
Test Start Date
pH 1st sample 1st sample 2nd sample
Control 7.5 7.5 7.4 7.6 7.6 7.5 Sample Type (Duration)
Treatment 2 7.6 7.9 7.7 8.0 7.7 7.7 Grab Duration 1st 2nd
Sample 1 24hrs.Tox ToxstartendstartendstartendSample 2 X 24hrs.DilutionD.O.1st sample 1st sample 2nd sample Hardness (mg/L)48.0Control8.0 8.1 8.2 8.3 8.1 8.8 Spec. Cond (pmhos)493Treatment 2 5578 8 8.3 8.5 8.6 8.8 8.8 Chlorine (mg/L)
SampleTemp. at receipt (°C)2.2LCSO/Acute Toxicity Test -0.2
(Mortality expressed as %. combin.ng replicates)
Concentration
[Mortality
LC50 =start/end start'end
Control
High Cone.
pH DO.
ETT Environmental. Inc.
5.627
2.508
57%
95% Confidence Limits
%
Method of Determination
Moving Average | [Probit
pearman Karber | [Other
Ceriodaphnia dubia
o%
Control
24.4
Control
Organism Tested
DEM Form AT-1
28-Nov-OS
County: WILSON
Comp
X
[09-Nov-05
Sample 2 11-10-05
^gaort Form - Chronic Pass/Faii and Acute l^)
™EK WWMF rZZZ ----
-------- NHDES# NC0023906 Pipe #
Comments
Sample Sample
UH
180
feWo-OSh) 05
Complete This for Either Test
Collection (Start) Date
Sample 1 11-7-05
Environmental Sciences Branch
<J> na.ci MUamy
LSL.lWf\ '*
iu^.1 ma., OC, V.ve uclllcl
v.a.vm.a A.vaa-iu*.,
Chronic Test Results
Calculated t=
[Critical Valuer
% Reduction=
Effluent Toxicity R;
Facility: WILSON HOMINY l
Laboratory Performing Test: ...
Signature of Operatorjn Responsible Charqe
x 4^-4-
[Signature of Laboratory Supervisor
MAIL ORIGINAL TO
% 3rd Brood PASS
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date:August 26, 2005
NPDES#: NC-0023906 Pipe ft 001 County: Wilson
Comments: 'C/7
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent
90%
FailPass
X
Test Start Date: 08-03-05
Sample 2 08-04-05
£
Duration
24.5-h
24,25-h
35
Control
High Cone.
pH (S.U.)DO (nig/L)7-daysCeriodaphnia dubia Duration:Organism Tested:
DiVQfonn AT-1 (3/87) rev. 11/95
Sample 1
Sample 2
.Alkalinity (mg CaCOj/L)
Hardness (mg CaCOj/L)
Conductivity (pnihos/cm)
Total Residual Chlorine (mgL)
Sample Temp at Receipt
Chronic Test Results
Calculated t:
Tabular t:
% Reduction:
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
PO Box 7565
Asheville, NC 28802
Concentration (%)
Mortality (%)
D.O. (mg/L)
Control
Treatment 2
E
3
pH (S.U.)
Control
Treatment 2
Treatment 2
30.3
~7.
■22
E
5
I 3 E a w
1 I
Control Organisms_________
Number of Young Produced
Adult Survival: (L)ive, (D)ead
Effluent Percentage
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
7,73
7.90
7.5
TN
1
24
L
7,73
7.85
7.6
7.6
4
27
L
5
27
T
7.65
7.93
6
28
L
8
32
L
Morality
Control
0.0
Average
Reproduction
Control
27.0
1st Sample
7.86
7.74
3
30
L
6
34
T
7,7
7.8
9
30
T
10
30
T
1st Sample
7.81
7.96
2nd Sample
7.66
7.94
494
<0.10
0.6
1st Sample
To
TT
26
L
12
31
T
1 st Sample
7.6
To
2
32
T"
3
28
L
4
32
L
2nd Sample
7.6
-7~4
7
27
L
7
26
L
8
29
T
10
26
L
11
27
L
11
32
T
Treatment 2
0.0
Control CV
12
28
T"
9
28
T
5.0
% c ontrol or gTOEtns
produentg 3rd brood
100
5
26
L
-4.264
2.508
-12.3
1
28
L
Sample Type/Duration
Grab Comp.
x-
X
Method of Determination
Trimmed Spearman Karber
Probit
Other:
LCS0/Acute Toxicity Test
(Mortality expressed as %, combining replicates.)
LC50 =
95% Confidence Limits
to
Project: 2005
Samples: 050803.03,050806.07
jgivironmental Testing Solomons, Inc.
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
46
160. 163,
16$
_____
0Q
Collection (Start) Date:
Sample 1 08-01-05
Facility Tritest. Inc.
Wilson WWTP
Laboratory Performing Test: Environmental Testing Solutions, Inc.
Signature of Operator in Responsible Charge: /[
Signature of Laboratory Supervisor: —
___________
_____
448
<0.10
0.6
Date
Comments
MAIL ORIGINAL TO
1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced 21 26 20 22 24 21 24 24 23 18 15 16 % Mortality Avg. Reprod.
L L L L LAdult (L)tve (D)ead L L L L L L L
Effluent %90.0%8%18.0
Treatment 2 Treatment 2
Control CV
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 16.1%io ii 12
# Young Produced 21 23 25 11 20 19 22 23 0 14 19 19 % 3rd Brood
L L L L LAdult (L)ive (D)ead L L L D L L L 83%
Complete This for Either Test
Collection (Start) Date 03-Aug-05
PH 1st sample 1st sample 2nd sample Sample 1 8-1-2005 Sample 2 8-4-2005
Control 7.6 7.4 7.2 7.7 7.7 7.2
Treatment 2 7.4 7.6 7.5 7.9 7.8 7.3 Grab Duration 1st 2nd
Sample 1 24hrs.Tox Tox
endstart start end XstartendSample 2 24hrs.Dilution Sample Sample
D.O.1st sample 1st sample 2nd sample Hardness (mg/L)49.9
Control 8.6 9.0 9.1 9.4 9.3 8.9 222Spec. Cond. (pmhos)441 512
Treatment 2 8.8 9.1 9.7 9.4 9.3 8.8 Chlorine (mg/L)0.08 <0.05
Sample Temp at receipt (°C)1.6 1.7
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
Concentration
Mortality start/end start/end
LC50 =Method of Determination Control
Probit95% Confidence Limits Moving Average High Cone.
%Spearman Karber pH D.O.
IOrganism Tested
DEM Form AT-1
128
109
15%
0%
Control
91 2
Control
[Other
Ceriodaphnia dubia
T ■.' ft
22-Aug-05
County: WILSON
Comp
T”
ETT Environmental, Inc.
Environmental Sciences Branch
UMV. Ul ITOICI WUQIILy
IV. UUI
IUAI IViail UCI YIUC X^villd
ivaictyii) ivvi mi vai viuia xi iu*. i
Chronic Test Results
Rank sum=
Critical Value=
% Reduction=
Effluent Toxicity - Chronic Pass/Fail and Acute
------------------------------------------ ------NPDES# NC0023906 Pipe#
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
Facility: WILSON HOMINY CREEK WWMF
Laboratory Performing Test:
.x _____ _ _______________
' Signature of Operator in Responsible Charge
■ X
Signature orOaboratory Supervisor
PASS FAIL
I X P"
Test Start Date
Date 8\17\05
Facility: Wilson 0023906NPDEStfNC Pipe#001
County Wilson
Laboratory Performing Test PACE Analytical Sen/ices, Inc,
Chronic Test ResultsIGINAL TO:Calculated t
0
7 9 . 10 11 12
# Young Produced 25 27 29 6 24 24 30 33 29 29 25 21
Adult (L)ive (D)ead L L L L L L L L L L L L
Effluent%90
1 2 3 4TREATMENT 2 ORGANISMS 5 6 7 8 9 10 11 12 PASS FAIL
# Young Produced 24 27 31 29 31 31 32 30 29 15 27 29 X
Check OneAdult (L)ive (D)ead L L L L L L L L L L L L
[Complete This For Either Test I1st1st2nd
Control Collection Start Date7.4 7.6pH 7.3 7.4 7.0 7.3
Treatment Sample 2 8\4\05
Samoel Tvoe/Duration
GraD Comp Duration
Sample 1ttt X 24.5hr
Sample 21st1st2nd X 24 25
D.O Control 8.1 8.0 8.4 8.2 7.9 7.6 Hardness (mg/1)48
Spec. Cond. tumhos)Treatment 7.9 7.7 8.1 7.9 7.7 7.5 417 469
Chlorine (mq/l)<0.1 <0.1
Samole temo. at receipt (C)
2.9 3.0
%%%%%% %%
Concentration
%%%%%%%%%
Mortality
LC50=%Method of Determination start/end start/end
95% Confidence Moving Avg.Probit Control
% -%Spearman Other
PH D.O.organism Tested: Ceriodaphnia dubia
Tabular t
% Reduction
Note: Please
Complete This
Section also
High
Cone.
12
37706
99006
J
o'
co tu
I
S’
Comments
Rank Sum=175.00
1-Tailed Critical=109.00
I % Mortality
Control
Avg. Reprod
Control
25.20
Treatment 2
27.90
7,4
s
t
a
7,5
E
n
d
7.2
s
t
a
7,5
E
n
d
LCSO/Acute Toxicity Test
rMortalitv expressed as % . combinina replicates)
%
7.4
E
n
d
7.5
s
t
a
0.00
Treatment 2
P.O
Control CV
I 27.3 |
%control organisms
producing 3rd brood
91.7
________ Samples Not Aerated Unless Otherwise Noted
Environmental Sciences Branch
Div. of Water Quality
N.C.'DENR
1621 Mail Service Center
Raleigh. North Carolina 27699-1621
Test Start Date
8\3\05
Ignature Of Operator in ResponstetmCharge \
' 7(
inn ire oflahoratorv Suftervisor A
Test Duration (Hours): .________
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
z
Charlotte Certification IDs
NC Wastewater
NC Drinking Water
SC
Asheville Certification IDs
NC Wastewater 40
NC Drinking Water 37712
SC Environmental 99030
f I
b Pace Analytical Services, Inc.
f 2225 Riverside Drive
» Asheville, NC 28804
Pass/Fail and Acute
rdX. Odu.ZOZ. 1 d
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS 1 2 3 4 5 6
mAnalyticar
www.pac^bW Toxicity RePort
Sample) g\1\05
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC5(,June 03, 2005Dale:
County: CliathiiruPipe #: MHNPDES#: NC- 0023906
Comments: £.’S?-~Li
North Carolina Ceriodaphnia Clironic Pass/Fail Reproduction Toxicity Test AveragePercent
LLLLLL
90%
FailPass
LLLLL
05-18-05Test Start Date:
05-19-05Sample 2
Duration
24 25-h
li-h
31
Concentration (%}
0.4
Mortality (%)
5
Control
Higli Cone.
DO (nig/L)pH (S.V.)6-daysDuration:Ceriodaphnia dubiciOrganism Tested:
DWQ fdFhrAT-T(T^7) rev. 11^5
I
D.O. (nig/L)
Control
Treatment 2
1
Simple 1
Sample 2
Alkalinity (mg CaCO3/L)
Hardness (nig CaCOvl.,i
Conductivity (pnhos'cni)
Total Residual Chlorine (Tng/L)
Sample Temp, at Receipt i"Ci
I
Chronic Test Results
Calculated t:
Tabular i:
% Reduction:
PO Box 7565
Asheville, NC 28802
pH (S.U.)
Control
Treatment 2
f
Treatment 2
32.0
1 1I
a
I
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQZ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
3
’W’
Morality
Control
0.0
Reproduction
Control
24.9
Control Organisms_________
Number of Young Produced
Adult Survival: (L)ive. (D)ead
7.9
7.8
7.8.-
7.9
7
23
L
7
34
T
8
31
L
Project:
Samples:
IS94
050518.03. 050521.05
Effluent Percentage
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
1
23
L
460
<C) 10
2
27
L
3
29
L
6
27
L
4S
151. 15o.
I6X
1st Sample
7.2217.3f
7.33 7.59
1st Sample
8.0
8.0
2
32
L
1
29
L
Treatment 2
0.0
Control CV
10.5
pioiiucing jid brood
100
-7.463
2.508
-28.4
415
:G. 10
0 6
8.1
8.2
Sample Type/Duration
Grab Comp.
X
9 10 11 12
30 34 | 32 | 35
T
Method of Determination
Trimmed Spearman Karber
Probit
Other:
LC5()/Acute Toxicity Test
(Mortality expressed as %, combining replicates.)
LC50=
95% Confidence Limits
to
4 5 6
32 32 34
L
3 4 5
22 | 22 | 28
~L
8 9 10 11 12
24 | 26 | 24 | 30 | 23
T
•h
2nd Sample
7.741 7.34
7.63 7.56
1st Sample
7.37 7.44
7.2? 7.80
1st Sample
7.9
7.9
2nd Sample
7.4
7.6
Collection (Start) Date:
Sample 1 05-16-05
^vironmental Testing Sol^jons, Inc. _
* Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Facility: Tritest, Inc.
Wilson WWTP
Laboratory Performing Test: Environmental Testing Solutions, Inc.
Signature of Operator in Responsible Charge: ;
Signature of Laboratory Supervisor: —■
Date 17-Feb-05
County: WILSONNPDES#
Comments
MAIL ORIGINAL TO
3 5 6 7 8 9 ' 10 11 12241
19 21 26 27 23 21 Avg. Reprod.25 22 27 23 23 13 % Mortalityit Young Produced
L L L L LLLL LLLLAdult (L)ive (D)ead
8%90.0%20.7Effluent %
Treatment 2 Treatment 2
Control CV
17.3%7 94568 10 11 12TREATMENT 2 ORGANISMS 1 2 3
26 22 23 23 22 22 % 3rd Brood PASS FAIL24221902223# Young Produced
XLLLLLLLL92%L LDLAdult (L)ive (D)ead
Complete This for Either Test Test Start Date
Collection (Start) Date 2-9-05
PH Sample 1 2-7-2005 Sample 2 2-10-20052nd sample1st sample1st sample
7.9 8.2 7.58.07.97.7Control Sample Type (Duration)
7.3 7.27.9 7.67.97.8 GrabTreatment 2 Duration 2nd1st
Sample 1 24hrs.Tox Tox
XSample 2 24hrs.Dilution Sample Sampleendstartendendstartstart
D.O.39.5Hardness (mg/L)2nd sample1st sample1st sample
8.2 461 5327.9 2358.4 8.08.58.3 Spec. Ccnd. (pmhos)Control
<0.05 <0.059.9 8.77.7 8.87.78.5 Chlorine (mg/L)Treatment 2
1.1 0.7Sample Temp, at receipt (°C)
LC50/Acute Toxicity Test
(Mortality expressed as %. combining replicates)
Concentration
start/end start/endMortality
Method of Determination ControlLC50 =
Probit High Cone.Moving Average95% Confidence Limits
D.O.PHOther%
Organism Tested
DEM Form AT-1
ETT Environmental, Inc.
139
109
8%
0%
Control
22.5
Control
Spearman Karber
Ceriodaphnia dubia
Comp
X
Environmental Sciences Branch
u/iv. ui vvaict xxuamy
I1.V. U'U.IVIV
iu£ i iviau xjci ’-ci nci
naiciyn, nui u i v>ai vm ia 4./ vuu- i ut i
Chronic Test Results
Rank sum=
Critical Value=
% Reduction=
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
-2.
•Facility: WICSON HOMINY C
Laboratory Performing Test: „
Signature of Operator in Responsible Charge
x______ _____________________
Signature of Laboratory Supervisor
Effluent Toxicity Reajg Form - Chronic Pass/Fail and Acute
^KkWWMF NPDES# NC0023906^npe #
Dale:February 24, 2005
NPDES #: NC- 0023906 Pipe #: 001 Countv: Wilson
1696
050209.1)5,050212.11
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent
L LL
Pass Fail
XLL
02-09-05Test Start Dale:
7.32
Sample 2 02-10-05
7.7 Duration
24.25-h
24 ||
<2,32 <5
2
to
Control
High Cone.
pH (S.V.)DO (mg/L)Duration:7-daysCeriodaphnia dubiaOrganism Tested:
DWQ form AT-1 (3/87) rev. 11/95
i
i
Method of Determination
Trimmed Spearman Karber
Probit
Other:
i
S.uipie 1
Sample 2
Alkalinity (mg CaCO/L)
Harilness i mg CaCO/L)
Co mine ti v ity 1 u r i iho s/c n i)
Total Residual Chlorine i.nig/L;
Satiple Tenp nt Receipt i',>C)
Chronic Test Results
Calculated t:
Tabular t:
% Reduction:
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
PO Box 7565
Asheville. NC 28302
Concentration (%)
Mortality (%)
D.O. (mg/L)
Control
Treatment 2
I I 1
3
pH (S.U.)
Control
Treatment 2
Treatment 2
33.2
I
8.8
% control ergwusns
producing 3rd brood
100
1
1
Morality
Control
0.0
Average
Reproduction
Control
28 3
Effluent Percentage [90% |
Treatment 2 Organisms
Number of Young Produced
Adult Survival: (L)ive, (D)ead
7,36
7.66
8.0
7.9
1
24
T
1
31
L
8.1
8.3
7
31
L
9
38
L
10
30
L
10
31
L
11
31
L
Project:
Sanpies:
Control Organisms_________
Number of Young Produced
Adult Survival: (L)ive, (Djcad
7,90
8.11
12
30
L
423
<0.10
0.7
2
26
L
2
3?
T
6
29
L
7
35
L
1st Sample
7.71
7.25
1st Sample
7,60
7.71
3
33
L
2nd Sample
8.00
8.17 7.66
12
29
L
1st Sample
7.8
TI
2nd Sample
8.0
8.4 8.1
8
34
L
499
<0.10
0.8
Treatment 2
0.0
Control CV
11
25
L
-4,622
2.508
-17.1
4>
• C. 144
IS?
4 5 6
37 32 35
T
Sample Type/Duration
Grab Conip.
3T
x
LC5a/Aeute Toxicity Test
(Mortality expressed as %, combining replicates.)
LC50=
95% Confidence Limits
II5^
8 9
29 30
L
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
3 4 5
29 | 26 | 32
L
1 st Sample
8.0
8.2
Comments: \
Collection (Start) Dale:
Sample 1 02-07-05
Facility: Tritest, Inc.
Wilson WWTP
Laboratory Performing Test: Environmental Testing Solutions, Inc,
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: ------ ---------------------
/ —————
E^ironmental Testing Solutions, Inc.
" Phone: [phone]
Fax: [phone]
E-mail: [e-mail])m
I
Facility: Wilson 0023906NPDEStfNC Pipe#001
County Wilson
Comments
MAIL ORIGINAL TO:-1.340
7 8 9 10 11
# Young Produced 24 29 25 21 22 22 26 28 27 23 23 Control Control
Adult (L)ive (D)ead L L L L L L L L L L L L
Effluent%90
1 2 3TREATMENT 2 ORGANISMS 4 5 6 7 8 9 10 11 12 PASS FAIL
# Young Produced 26 26 25 28 22 26 27 26 25 31 27 23 X
Adult (L)ive (D)ead Check OneLLLLLLLLLLLL
[Complete This For Either Test ]1st 1st 2nd
Control 7.2 7.2 Collection Start Date7.2pH 7.4 7.4 7.6
Treatment Sample 17.6 Sample 22\7\05 2\10\05
Samoel Tvoe/Duration
Sample 1ttt X 24.25hr
Sample 21st1st2nd X 24hr
D.O Control 7.8 7.7 8.1 7.8 8.0 7.7 Hardness (mg/l)50
Spec. Cond. (umtios)Treatment 8.4 8.2 8.7 8.5 8.6 8.3 399 408
Chlorine (mq/l)<0.1 <0.1
Samole temp at receipt (C)
0.6 2.5
%%%%%%%%
Concentration
%%%%%%%%%
Mortality
LC50=%Method of Determination start/end start/end
95% Confidence Moving Avg.Probit Control
% -%Spearman Other
pH D~O~Organism Tested: Ceriodaphnia dubia
Tabular t
% Reduction
99030
E87648
High
Cone.
%control organisms
producing 3rd brood
| 100.0
E
n
d
t
12
37706
99006
CO7C-17
4
Note: Please
Complete This
Section also
2.508
0
LCSO/Acute Toxicity Test
(Mortality expressed as % . combinino replicates)
%
7.9
E
n
d
24.70
Treatment 2
26.00
7,4
E
n
d
7.4
s
t
a
0.00
Treatment 2
0.0
Control C.V
I 10.4 I
7.3
S
t
a
7.6
S
t
a
[Test Start Date.
2\9\05
vce Analytic^
Chronic Test Results
Calculated t
_____ Samples Not Aerated Unless Otherwise Noted
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Charlotte Certification IDs
NC Wastewater
NG Drinking Water
SC
Pl MCI AD
137
1
Test Duration (Hours): .________
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, Inc.
•Y
■nehc
a
g
I
Pace Analytical Services. Inc.
2225 Riverside Drive
Asheville, NC 28804
www.pac^S^^ Toxicity Report AT-1 Form Chronic Pass/Fail and Acute i_C5^one: 828-25^71^
Date 2\28\05 Fax: [phone]
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS 1 2 3 4 5 6 12 I____
| % Mortality ~ Avg. Reprod.
26
CJ Q.
M —1 h SI
S. <D
Asheville Certification IDs
NC Wastewater 40
NC Drinking Water 37712
SC Environmental
FL MELAP
Grab Comp. Duration
Laboratory Performing Test PACE Analytical Services, Inc.
Signature of Operator in Responsible Charge
Ix t, 1 ---- 2__________________
Sicnatfrrm of Lannratnrv Si loervisor