NC0021601_Renewal (Application)_20240705

ROY COOPER
Governor , I -..'\fit
ELIZABETH S.BISER [r r,^* . %
Secretary ,�`
RICHARD E.ROGERS,JR. NORTH CAROLINA
Director Environmental Quality
July 08, 2024
Town of Tryon
Attn: Gregory McCool
301 N Trade St
Tryon, NC 28782
Subject: Permit Renewal
Application No. NC0021601
Tryon WWTP
Polk County
Dear Applicant:
The Water Quality Permitting Section acknowledges the July 8, 2024, receipt of your permit renewal application and
supporting documentation. Your application will be assigned to a permit writer within the Section's NPDES WW permitting
branch. Per G.S. 150B-3 your current permit does not expire until permit decision on the application is made.
Continuation of the current permit is contingent on timely and sufficient application for renewal of the current permit. The
permit writer will contact you if additional information is required to complete your permit renewal. Please respond in a
timely manner to requests for additional information necessary to allow a complete review of the application and renewal
of the permit.
Information regarding the status of your renewal application can be found online using the Department of Environmental
Quality's Environmental Application Tracker at:
https://www.deq.nc.gov/permits-rules/environmental-application-tracker
If you have any additional questions about the permit, please contact the primary reviewer of the application using the
links available within the Application Tracker.
Since y,
Wren hedford
Administrative Assistant
Water Quality Permitting Section
cc: Kurt Wright-SDG Engineering
ec: WQPS Laserfiche File w/application
DEQ North Carolina Department of Environmental Quality I Division of Water Resources
./ Ashevl/e Regional Office 2090 U5.Highway 70 Swannanoa North Carolina 28778
/ 82820h 4500

ss
S t NGI N EERI NG
163 Heritage Lane,Bostic,NC 28018•828.245.4080 office•828.223.2265 mobile•828.245.2189 fax
June 27, 2024
NC Division of Water Resources
Water Quality Permitting Section—NPDES
1617 Mail Service Center RECEIVED
Raleigh, NC 27699-1617
JUL 0 5 2024
RE: Town of Tryon WWTP DWRMpDES
NPDES Permit Number NC0021601 NCDEQr
NPDES Permit Renewal Application
Town of Tryon, NC
NC Division of Water Resources,
The purpose of this transmittal letter is to submit the Tryon WWTP NPDES permit renewal
application (application) to the NC Division of Water Resources, Water Quality Permitting Section.
SDG Engineering, Inc. (SDG) prepared the Town of Tryon (TOT) application.
As part of this application,The Town of Tryon would like to request a change from Flowproportional
Composite sampling to a Constant Time/Constant Volume based composite sampling.
Per guidance from Gary Perlmutter, flow-proportional composite sampling would be expected for a
facility with substantial intermittent flow which is not expected to be the case for the Town of
Tryon based on their service area and minimal variability in the resulting flow profile.
A mercury minimization plan has not been included because the design flowrate of the POTW is 1.5
Mgpd which is below the 2 Mgpd threshold established in the requirements listed in the "NC Model
Mercury Minimization Plan" document listed under the "Mercury Guidance" section of the NC
Water Resource Division DEQ website.
Three sets of the enclosed documents include:
1. EPA Form 2A completed by SDG, reviewed by the WWTP ORC, and executed by Greg McCool,
Public Works Director
2. Process Narrative
3. Process Flow Diagram of the WWTP

4. USGS Map showing the Discharge Point to Vaughn Creek
5. Four Effluent Pollutant Scans utilized for the application. (6/11/19, 6/8/21, 6/7/22, 12/12/23)
6. Six WET testing documents, Ceriodaphnia dubia and Pimephales promelas, utilized for the
application.
7. Biosolids Management Plan (BMP) narrative
Should you require any additional information, please feel free to contact me.
Sincerely,
Kurt Wright, PE, BCEE, PWAM
President
Enclosures
Cc: Mr. Jim Fatland,Town Manager; Mr. Greg McCool, Public Works Director, Ms. Deborah Bradley,
WWTP ORC

Process Narrative -Town of Tryon Wastewater Treatment Plant,
NPDES Permit No. NC0021601
The Town of Tryon Wastewater Treatment Plant is located at 1526 East Howard Street in Tryon, NC
28782, in Polk County. The oldest available online records show an original design capacity of 0.8 MGD
in the 1970's. The plant underwent an expansion in the mid-to-late 1980s which added mechanical and
manual bypass bar screening, an aerated grit unit, a Parshall flume, an aeration basin, a secondary
clarifier, a sludge pump station, a sludge dewatering system, flow measurement, and UV light
disinfection. This expansion increased its capacity to 1.5 MGD. Records indicate that authorization to
construct a Chlorine contact basin with diffusion equipment was granted in 1990. UV light disinfection is
no longer in use at the plant and was abandoned in lieu of chlorine disinfection. Correspondence between
the Town of Tryon ORC and the permit preparer indicates that the aerated grit removal unit was
inoperable and disconnected from the system in the 1990's.
Influent from the municipality's upstream collection system enters the WWTP and flows to a mechanically
cleaned bar screen. A back-up manual bar screen is present for redundancy. Flow is measured via a
Parshall flume. Raw wastewater, supernatant from the aerobic digester, and return activated sludge are
then added to the aeration basin. Following the aeration basin, there a splitter box which diverts flow to
two clarifiers. Effluent from the clarifiers adjoins at a tie point and flows to a Chlorine contact chamber
which utilizes Chlorine diffusion equipment. The wastewater is then de-chlorinated. Flow continues to
Outfall 001 which discharges into Vaughn Creek.
Return sludge is pumped from a pump building just downstream of the clarifiers back to the aeration
basin. Waste sludge and scum are pumped to the aerobic digester. Following digestion, the thickened
sludge is sent to a sludge holding pond and eventually loaded into trucks for land application. Two fields
have been approved for application of Class B biosolids and are listed in Appendix B of permit
WO0004341.
Wastewater composition at the site currently has a larger percentage of domestic sources than in
previous decades. A 1977 Assimilative Capacity evaluation showed an anticipated 50%/50% split
between domestic and industrial flow. A waste load allocation document from 1985 shows a shift to 66%
domestic and 33% industrial flow. The site currently only has a single SIU—Tryon Finishing Corporation,
previously Carolina Yarn Processing. Permit issuance data from 2009 show an SIU process water
flowrate of 0.2 MGD and a non-process flowrate of 10,000 gpd. Industrial flows have decreased
dramatically in recent years.A June, 2023 industrial user survey showed a process flow-rate of 30,000
gpd, and a non-process-flowrate of 6,000 gpd. Based on average annualized daily flows, this would
amount to an influent concentration of 10-15%from industrial sources.
The site is actively garnering funding for a preliminary engineering report(PER)to determine where their
efforts on rehabilitating and/or replacing portions of the wastewater treatment plant should be focused. In
advance of this(PER)the facility plans to prioritize replacement of the clarifier weirs, and of the aerator in
the aerobic digester. Efforts are ongoing to replace portions of the collection system upstream of the
WWTP in order to reduce Inflow and infiltration. In July of 2021, the Town of Tryon submitted an
advertisement for bid for 3,310 linear feet for sewer pipe replacement and rehabilitation.
1 I Page

Town of Tryon, NC Wastewater Treatment Plant
Permit# NC0021601
A ►' Clarifier
Mech. Flow ' Aeration Splitter l Chlorine Effluent
Influent Cleaned —0 Contact —' Disposal to
Meas. • Basin Box
Bar Screen Chamber& Vaughn
Clarifier ) Dechlorination Creek
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♦ +
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•
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Quad:Landrum,S.C.-N.C. N C 0 0 216 01 Facility
Subbasin: 30806 location
Town of Tryon WWTP
I atitude:35°12'46"
Longitude:82°13'09"
Receiving Stream:Vaughn Creek SCALE 1:24000
Stream Class:C North

Biosolids Management Plan -Town of Tryon Wastewater Treatment Plant,
NPDES Permit No. NC0021601
The Town of Tryon Wastewater Treatment Plant(WWTP) operates under NPDES permit NC0021601 and
disposes of Class B residuals by land application under permit WO0004341. Pursuant to section II of the
land application permit the site must maintain the residuals management program without discharge to
surface waters and within the regulatory framework of surface or groundwater standards. Town of Tryon
WWTP is permitted to apply Class B biosolids to Polk County parcels P49-13 and T12-E17 with a total
net acreage of 14.1 acres.
The facility utilizes a suspended growth activated sludge process for wastewater treatment. Following the
aeration basin, flow is split and sent to two circular clarifiers. Sludge from the two secondary clarifiers is
gravity fed to a wet well, where it is pumped as return activated sludge back to the aeration basin or
waste activated sludge to the aerobic digester. The aerobic digester is 25 ft in diameter and 18 ft deep,
made of concrete construction and has a capacity of 66,057 gal. The digester is equipped with (3)4" plug
valves used to decant the supernatant and send it back to the aeration basin. Thickened waste sludge is
gravity fed from the digester to the sludge holding basin which is also made of concrete construction. The
holding basin is equipped with a 15 HP mixer.
The site uses a time-based schedule for wasting sludge. Sludge is sent to the digestor on Monday,
Wednesday, and Friday of each week. The volume of sludge pumped from the clarifiers to the digestor is
based on digestor level. When the digester is filled, the operators halt the process.
Waste sludge is loaded from the holding basin onto a truck and transported to the land application sites.
The Town operates the truck used to land apply the biosolids. Biosolids application is performed in
accordance with the requirements of WO0004341.
1 I PAGE

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
Form U.S.Environmental Protection Agency
2A aEPA Application for NPDES Permit to Discharge Wastewater
NPDES NEW AND EXISTING PUBLICLY OWNED TREATMENT WORKS
SECTION 1. BASIC APPLICATION INFORMATION FOR ALL APPLICANTS(40 CFR 122.21(J)(1)AND(9))
1.1 Facility name
Tryon Wastewater Treatment Plant(WWTP)
Mailing address(street or P.O.box)
1586 East Howard Street
City or town State ZIP code
Tryon NC 28782
Contact name(first and last) Title Phone number Email address
s Wastewater Plant Supervisor [e-mail]
Deborah Bradley &ORC [phone] m
0
Location address(street, route number,or other specific identifier) ® Same as mailing address
u_ City or town State ZIP code
1-2 Is this application for a facility that has yet to commence discharge?
❑ Yes 4 See instructions on data submission [Si No
Requirements for new dischargers
1.3 Is applicant different from entity listed under Item 1.1 above?
❑ Yes ® No 4 SKIP to Item 1.4
Applicant name
Town of Tryon
Applicant address(street or P.O. box)
0 301 North Trade Street
City or town State ZIP code
Tryon NC 28782
Contact name(first and last) Title Phone number Email address
Deborah Bradley Wastewater Plant Supervisor [phone] [e-mail]
1.4 Is the applicant the facility's owner,operator,or both?(Check only one response.)
❑ Owner ❑ Operator ® Both
1.5 To which entity should the NPDES permitting authority send correspondence?(Check only one response.)
❑ Facility ❑ Applicant ® Facility and applicant
(they are one and the same)
1.6 Indicate below any existing environmental permits.(Check all that apply and print or type the corresponding permit
number for each.)
Existing Environmental Permits
o_ ®NPDES(discharges to surface ❑ RCRA(hazardous waste) ❑ UIC(ul)dcrground injection
con
water)
NC0021601
2 ❑ I'SD(air emissions) ❑ Nonattainment program(CM) ❑ NESHAPs(CM)
rn
'� ❑ El Other (specify)
) WQ0004341 Land Application
EPA Form 3510-2A Page 1

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
17 Provide the collection system information requested below for the treatment works.
Municipalit Population Collection System Type Ownership Status
y Served Served (indicate percentage)
Town of Tryon 1,562:based on 100 %separate sanitary sewer r Own ❑ Maintain
2020 US Census %combined storm and sanitary sewer ❑ Own ❑ Maintain
0 Unknown ❑ Own ❑ Maintain
N %separate sanitary sewer 0 Own ❑ Maintain
c %combined storm and sanitary sewer CIOwn 0 Maintain
❑ Unknown ❑ Own ❑ Maintain
%separate sanitary sewer ❑ Own ❑ Maintain
0
ao %combined storm and sanitary sewer 0 Own 0 Maintain
c CI Unknown 0 Own ID Maintain
%separate sanitary sewer ❑ Own ❑ Maintain
; %combined storm and sanitary sewer ❑ Own El Maintain
co CIUnknown ❑ Own ❑ Maintain
c Total 1,562
Populatio
°' n
coi Served
Combined Storm and
Separate Sanitary Sewer System Sanitary Sewer
Total percentage of each type of 100 % 0
/0 sewer line(in miles)
Z' 18 Is the treatment works located in Indian Country?
c
0
0 Yes ® No
c19 Does the facility discharge to a receiving water that flows through Indian Country?
a c ❑ Yes ® No
1.10 Provide design and actual flow rates in the designated spaces. Design Flow Rate
1.5 mgd
Annual Average Flow Rates
(Actual)
d 2021 2022 2023
1O
c c 0.274 mgd 0.246 mgd 0.324 mgd
co Maximum Daily Flow Rates(Actual)
0 2021 2022 2023
1.58 mgd 1.13 mgd 1.90 mgd
1.11 Provide the total number of effluent discharge points to waters of the United States by type.
a Total Number of Effluent Discharge Points by Type
i- Constructed
d ,, Combined Sewer
E .0 Treated Effluent Untreated Effluent Overflows Bypasses Emergency
_cr Overflows
U C_
N -o
b a 1 0 0 0
EPA Form 3510-2A Page 2

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
Outfalls Other Than to Waters of the United States
1.12 Does the POTW discharge wastewater to basins,ponds,or other surface impoundments that do not have outlets for
discharge to waters of the United States?
❑ Yes No-4 SKIP to Item 1.14.
1.13 Provide the location of each surface impoundment and associated discharge information in the table below.
Surface Impoundment Location and Discharge Data
Average Daily Volume Continuous or Intermittent
Location Discharged to Surface
Impoundment
(check one)
❑ Continuous
gpd ❑ Intermittent
❑ Continuous
gpd ❑ Intermittent
0 Continuous
gpd 0 Intermittent
0
1.14 Is wastewater applied to land?
❑ Yes ® No 4 SKIP to Item 1.16.
u) 1.15 Provide the land application site and discharge data requested below.
0 Land Application Site and Discharge Data
Continuous or
Location Size Average Daily Volume Intermittent
Applied (check one)
❑ Continuous
❑ Intermittent
0
0 Continuous
❑ Intermittent
❑ Continuous
❑ Intermittent
1.16 Is effluent transported to another facility for treatment prior to discharge?
o ❑ Yes No 4 SKIP to Item 1.21.
1.17 Describe the means by which the effluent is transported(e.g.,tank truck,pipe).
1.18 Is the effluent transported by a party other than the applicant?
❑ Yes ❑ No—) SKIP to Item 1.20.
1.19 Provide information on the transporter below.
Transporter Data
Entity name Mailing address(street or P.O.box)
City or town State ZIP code
Contact name(first and last) Title
Phone number Email address
EPA Form 3510-2A Page 3

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
1.20 In the table below,indicate the name,address,contact information, NPDES number,and average daily flow rate of the
receiving facility.
Receiving Facility Data
0 Facility name Mailing address(street or P.O.box)
City or town State ZIP code
0
U
Contact name(first and last) Title
0
Phone number Email address
flNPDES number of receiving facility(if any) ❑None Average daily flow rate mgd
O 1.21 Is the wastewater disposed of in a manner other than those already mentioned in Items 1.14 through 1.21 that do not have
`o outlets to waters of the United States(e.g.,underground percolation,underground injection)?
coYes -4 IZI No SKIP to Item 1.23.
❑
cn 1.22 Provide information in the table below on these other disposal methods.
Information on Other Disposal Methods
oDisposal Location of Size of Annual Average Continuous or Intermittent
Method Disposal Site Disposal Site Daily Discharge (check one)
Description Volume
El Continuous
acres gPd ❑ Intermittent
o ❑ Continuous
acres gPd ❑ Intermittent
d ❑ Continuous
acres
gP ❑ Intermittent
1.23 Do you intend to request or renew one or more of the variances authorized at 40 CFR 122.21(n)?(Check all that apply.
Consult with your NPDES permitting authority to determine what information needs to be submitted and when.)
c y
Discharges into marine waters(CWA
c _ ❑ ❑ Water quality related effluent limitation(CWA Section 302(b)(2))
cr Section 301 h
® Not applicable
1.24 Are any operational or maintenance aspects(related to wastewater treatment and effluent quality)of the treatment works
the responsibility of a contractor?
❑ Yes ® No 4SKIP to Section 2.
1.25 Provide location and contact information for each contractor in addition to a description of the contractor's operational and
maintenance responsibilities.
Contractor Information
Contractor 1 Contractor 2 Contractor 3
O Contractor name
(company name)
Mailing address
(street or P.O.box)
City,state,and ZIP code
Contact name(first and
O last)
U
Phone number
Email address
Operational and
maintenance
responsibilities of
contractor
EPA Form 3510-2A Page 4

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
SECTION 2.ADDITIONAL INFORMATION(40 CFR 122.21(J)(1)AND(2))
o Outfalls to Waters of the United States
2.1 Does the treatment works have a design flow greater than or equal to 0.1 mgd?
0 11 Yes ❑ No 4 SKIP to Section 3.
2_2 Provide the treatment works'current average daily volume of inflow Average Daily Volume of Inflow and Infiltration
and infiltration. 20,000 gpd
Indicate the steps the facility is taking to minimize inflow and infiltration.
Line replacements and repairs are both underway and planned.
The process of bidding and contracting out the tasks below was initiated in 2021:
- Installation of approximately 2,730 LF of 8"PVC SDR 35 sanitary sewer mains
- Installation of approximately 140 LF of 8"DIP sanitary sewer mains
- Installation of approximately 50 LF of 16"steel encasement pipe with 8"DIP sanitary sewer main
- Installation of approximately 26 manholes
- Installation of approximately 390 LF of 6"CIPP lining
- Residential service reconnections for water line and sewer mains
La
3 The Town of Tryon staff have listed four projects which are ongoing or about to start,for line repairs and replacements
including Hidden Hill Rd.,Markham Rd.,Oak Street,and Braewick Rd.
2_3 Have you attached a topographic map to this application that contains all the required information?(See instructions for
a) Ct. specific requirements.)
m R
p
Q
11 Yes
E 22 4 Have you attached a process flow diagram or schematic to this application that contains all the required information?(See
o 2 instructions for specific requirements.)
• as
Yes
2_5 Are improvements to the facility scheduled?
-0 Yes ❑ No 4 SKIP to Section 3.
d
in Briefly list and describe the scheduled improvements.
The Town of Tryon is currently in the funding phase for a Preliminary Engineering Report(PER)which will provide
recommendations for repairs and improvements or replacements of the existing POTW.Appropriate permits will be obtained
for future improvements following design.
c
Q 0 1. Clarifier weir replacement
E 16
d 2. Aerator replacement in the aerobic digestor
13
cn o 3.
2.6 Provide scheduled or actual dates of completion for improvements.
Scheduled or Actual Dates of Completion for Improvements
Scheduled Affected Attainment of
Begin End Begin
Outfalls Operational
Improvement Construction Construction Discharge
(from above) (list outfall (MM/DD/YYYY) (MM/DD/YYYY) (MM/DD/YYYY) Level
number (MM/DD/YYYY)
06/2026 1
001
2. 06/2026
001
EPA Form 3510-2A Page 5

3.
4.
2_7 Have appropriate permits/clearances concerning other federal/state requirements been obtained?Briefly explain your
response.
❑ Yes ❑ No ® None required or applicable
Explanation: See response in 2.5
EPA Form 3510-2A Page 6

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
SECTION 3.INFORMATION ON EFFLUENT DISCHARGES(40 CFR 122.21(J)(3)TO(5))
3.1 Provide the following information for each outfall. (Attach additional sheets if you have more than three outfalls.)
Outfall Number 001 Outfall Number Outfall Number
State North Carolina
County Polk
City or town Tryon
0
o Distance from shore Outlet structure is at the bank ft. ft.
Depth below surface Bottom of outlet pipe is—3.5 ft ft. ft.
'a P P�P
above water level
o Average daily flow rate 0.298 mgd mgd mgd
**4.5 years of data**
Latitude 35°12'46"N
Longitude 82°13'9"W
3.2 Do any of the outfalls described under Item 3.1 have seasonal or periodic discharges?
o ❑ Yes ® No 4 SKIP to Item 3.4.
ct,' 3_3 If so,provide the following information for each applicable outfall.
y Outfall Number 001 Outfall Number Outfall Number
Number of times per year
s discharge occurs
a Average duration of each
discharge(specify units)
Tts o Average flow of each mgd mgd mgd
discharge
a) Months in which discharge
cn
occurs
3_4 Are any of the outfalls listed under Item 3.1 equipped with a diffuser?
❑ Yes IZI No 4 SKIP to Item 3.6.
3.5 Briefly describe the diffuser type at each applicable outfall.
Outfall Number Outfall Number Outfall Number
'Cr)
U)
0 3_6 Does the treatment works discharge or plan to discharge wastewater to waters of the United States from one or more
vi
discharge points?
® Yes ❑ No 4 SKIP to Section 6.
EPA Form 3510-2A Page 6

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
3.7 Provide the receiving water and related information(if known)for each outfall.
Outfall Number 001 Outfall Number Outfall Number
Receiving water name
Vaughn Creek to Pacolet
River to Broad River
Name of watershed,river,
or stream system
Broad
Natural Resources
o Conservation Service 14-
c digit watershed code HUC 12-030501051201
(from USGS)
Name of state
management/river basin
Broad
U.S.Geological Survey
d 8-digit hydrologic
cataloging unit code 03050105
re
Critical low flow(acute) 4 cfs cfs cfs
Critical low flow(chronic) 5 cfs cfs cfs
Total hardness at critical mglL of mg/L of
low flow 22.1 mg/L of CaCO3 CaCO3 CaCO3
3 8 Provide the following information describing the treatment provided for discharges from each outfall.
Outfall Number 001 Outfall Number Outfall Number
Highest Level of ❑ Primary 0 Primary ❑ Primary
Treatment(check all that ❑ Equivalent to 0 Equivalent to ❑ Equivalent to
apply per outfall) secondary secondary secondary
® Seeurrddiy ❑ Secor,ddry ❑ Secunddry
❑ Advanced ❑ Advanced ❑ Advanced
❑ Other(specify) ❑ Other(specify) 0 Other(specify)
0
Q Design Removal Rates by
Outfall
BOD5 or CBOD5 85 /° 0/0
Cd
I
TSS 85 %
® Not applicable 0 Not applicable ❑ Not applicable
Phosphorus /o
® Not applicable ❑ Not applicable ❑ Not applicable
Nitrogen
Other(specify) ❑ Not applicable ❑ Not applicable ❑ Not applicable
EPA Form 3510-2A Page 7

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
3.9 Describe the type of disinfection used for the effluent from each outfall in the table below. If disinfection varies by season,
d describe in the table below.
4 Outfall Number 001 Outfall Number Outfall Number
0
c) Disinfection type
O Chlorination
.5 ❑Seasons used
cn
Dechlorination used? ❑ Not applicable ❑ Not applicable ❑ Not applicable
►Z1 Yes 0 Yes 0 Yes
❑ No ❑ No 0 No
3.10 Have you completed monitoring for all Table A parameters and attached the results to the application package?
L Yes
3.11 Have you conducted any WET tests during the 4.5 years prior to the date of the application on any of the facility's
discharges or on any receiving water near the discharge points?
® Yes ❑ No 4 SKIP to Item 3.13.
3.12 Indicate the number of acute and chronic WET tests conducted since the last permit reissuance of the facility's discharges
by outfall number or of the receiving water near the discharge points.
Outfall Number 001 Outfall Number Outfall Number
Acute Chronic Acute Chronic Acute Chronic
Number of tests of discharge
water 0 20
Number of tests of receiving
water
3.13 Does the treatment works have a design flow greater than or equal to 0.1 mgd?
O 1� Yes ❑ No 4 SKIP to Item 3.16.
o) 3.14 Does the POTW use chlorine for disinfection,use chlorine elsewhere in the treatment process,or otherwise have
reasonable potential to discharge chlorine in its effluent?
►� Yes 4 Complete Table B,including chlorine. ❑ No 4 Complete Table B,omitting chlorine.
3 315 Have you completed monitoring for all applicable Table B pollutants and attached the results to this application package?
w ® Yes
3.16 Does one or more of the following conditions apply?
• The facility has a design flow greater than or equal to 1 mgd.
• The POTW has an approved pretreatment program or is required to develop such a program.
• The NPDES permitting authority has informed the POTW that it must sample for the parameters in Table C,must
sample other additional parameters(Table D),or submit the results of WET tests for acute or chronic toxicity for each
of its discharge outfalls(Table E).
11 Yes 4 Complete Tables C,D,and E as applicable. ❑ No SKIP to Section 4.
3.17 Have you completed monitoring for all Table C pollutants and attached the results to this application package?
►� Yes
3.18 Have you completed monitoring for all Table D pollutants required by your NPDES permitting authority and attached the
results to this application package?
4 Yes El No additional sampling required by NPDES
permitting authority.
EPA Form 3510-2A Page 8

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
3.19 Has the POTW conducted either(1)minimum of four quarterly WET tests for one year preceding this permit application or
(2)at least four annual WET tests in the past 4.5 years?
Yes ❑ No 4 Complete tests and Table E and SKIPto
Item 3.26.
3.20 Have you previously submitted the results of the above tests to your NPDES permitting authority?
❑ Yes 0No 4 Provide results in Table E and SKIP to
Item 3.26.
3.21 Indicate the dates the data were submitted to your NPDES permitting authority and provide a summary of the results.
Date(s)Submitted Summary of Results
(MM/DD/YYYY)
a
O
w3.22 Regardless of how you provided your WET testing data to the NPDES permitting authority,did any of the tests result in
co
xi i to c ty
❑ Yes ►1 No 4 SKIP to Item 3.26.
co 3.23 Describe the cause(s)of the toxicity:
d
W
3.24 Has the treatment works conducted a toxicity reduction evaluation?
❑ Yes ® No 4 SKIP to Item 3.26.
3.25 Provide details of any toxicity reduction evaluations conducted.
3.26 Have you completed Table E for all applicable outfalls and attached the results to the application package?
/1 Yes ❑ Not applicable because previously submitted information to the NPDES permitting authority.
SECTION 4.INDUSTRIAL DISCHARGES AND HAZARDOUS WASTES(40 CFR 122.21(J)(6)AND(7))
4.1 Does the POTW receive discharges from SIUs or NSCIUs?(See instructions for definitions of SlUs and NSCIUs.)
@ Yes ❑ No 4 SKIP to Item 4.7.
4.2 Indicate the number of SIUs and NSCIUs that discharge to the POTW.
Number of SlUs Number of NSCIUs
N
O
2 4.3 Does the POTW have an approved pretreatment program?
co
_ ►� Yes ❑ No
o 4.4 Have you submitted either of the following to the NPDES permitting authority that contains information substantially
identical to that required in Table F:(1)a pretreatment program annual report submitted within one year of the application
or(2)a pretreatment program?
@ Yes ❑ No 4 SKIP to Item 4.6.
o 4.5 Identify the title and date of the annual report or pretreatment program referenced in Item 4.4.SKIP to Item 4.7.
Town of Tryon Wastewater Treatment Plant 2023 PAR—dated 2/12/2024,signed by Deborah Bradley.Attn:Octavio
cHenriquez-Pretreatment Coordinator—PERCS NC Division of Water Resources
4.6 Have you completed and attached Table F to this application package?
14 Yes
EPA Form 3510-2A Page 9

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
4_7 Does the POTW receive,or has it been notified that it will receive, by truck,rail,or dedicated pipe,any wastes that are
regulated as RCRA hazardous wastes pursuant to 40 CFR 261?
❑ Yes ® No 4 SKIP to Item 4.9.
4.8 If yes,provide the following information:
Annual
Hazardous Waste Waste Transport Method Amount of Units
Number (check all that apply) Waste
Received
❑ Truck ❑ Rail
❑ Dedicated pipe ❑ Other(specify)
0
❑ Truck ❑ Rail
❑ Dedicated pipe ❑ Other(specify)
0
v
N ❑ Truck ❑ Rail
a,
_ ❑ Dedicated pipe ❑ Other(specify)
43
d
4-9 Does the POTW receive,or has it been notified that it will receive,wastewaters that originate from remedial activities,
including those undertaken pursuant to CERCLA and Sections 3004(7)or 3008(h)of RCRA?
❑ Yes ® No 4 SKIP to Section 5.
11) 4.10 Does the POTW receive(or expect to receive)less than 15 kilograms per month of non-acute hazardous wastes as
specified in 40 CFR 261.30(d)and 261.33(e)?
❑ Yes 4 SKIP to Section 5. ❑ No
4.11 Have you reported the following information in an attachment to this application:identification and description of the site(s)
or facility(ies)at which the wastewater originates;the identities of the wastewater's hazardous constituents;and the extent
of treatment,if any,the wastewater receives or will receive before entering the POTW?
El Yes
SECTION 5.COMBINED SEWER OVERFLOWS(40 CFR 122.21(J)(8))
5_1 Does the treatment works have a combined sewer system?
❑ Yes ® No 4 SKIP to Section 6.
5.2 Have you attached a CSO system map to this application?(See instructions for map requirements.)
c ❑ Yes
5.3 Have you attached a CSO system diagram to this application?(See instructions for diagram requirements.)
❑ Yes
EPA Form 3510-2A Page 10

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
5.4 For each CSO outfall, provide the following information.(Attach additional sheets as necessary.)
CSO Outfall Number CSO Outfall Number CSO Outfall Number
City or town
0
a State and ZIP code
0
w
d
County
o Latitude
0
Longitude
Distance from shore ft. ft. ft.
Depth below surface ft. ft. ft.
5.5 Did the POTW monitor any of the following items in the past year for its CSO outfalls?
CSO Outfall Number CSO Outfall Number CSO Outfall Number
Rainfall ❑ Yes❑No ❑ Yes 0 No 0 Yes❑No
rn
0 CSO flow volume ❑ Yes❑No ❑ Yes❑No ❑ Yes❑No
CSO pollutant Cl Yes 0 No 0 Yes 0 No 0 Yes ❑No
concentrations
Receiving water quality ❑ Yes 0 No ❑ Yes 0 No ❑ Yes❑No
CSO frequency ❑ Yes ❑No ❑ Yes 0 No ❑ Yes 0 No
Number of storm events 0 Yes❑No 0 Yes 0 No ❑ Yes❑No
5_6 Provide the following information for each of your CSO outfalls.
CSO Outfall Number CSO Outfall Number_ CSO Outfall Number
} Number of CSO events in events events events
the past year
A
Average duration per hours hours hours
VI
event ❑Actual or❑ Estimated 0 Actual or 0 Estimated ❑Actual or 0 Estimated
w million gallons million gallons million gallons
Average volume per event
0 0 Actual or❑ Estimated ❑Actual or❑ Estimated ❑Actual or❑Estimated
Minimum rainfall causing a inches of rainfall inches of rainfall inches of rainfall
CSO event in last year ❑Actual or❑ Estimated 0 Actual or❑Estimated ❑Actual or❑ Estimated
EPA Form 3510-2A Page 11

EPA Identification Number I NPDES Permit Number Facility Name
Town of Tryon OMB No.2040 0004
NC0021601 Expires 07/31/2026
5.7 Provide the information in the table below for each of your CSO outfalls.
CSO Outfall Number CSO Outfall Number CSO Outfall Number_
Receiving water name
Name of watershed/
stream system
dNatural Resources 0 Unknown ❑Unknown ❑Unknown
i Conservation Service 14-
digit watershed code
(if known)
d Name of state
re management/river basin
o
en U.S.Geological Survey ❑ Unknown ❑ Unknown
8-Digit Hydrologic Unit ❑Unknown
Code(if known)
Description of known
water quality impacts on
receiving stream by CSO
(see instructions for
examples
SECTION 6. CHECKLIST AND CERTIFICATION STATEMENT(40 CFR 122.22(A)AND(D))
6_1 In Column 1 below,mark the sections of Form 2A that you have completed and are submitting with your application.For
each section,specify in Column 2 any attachments that you are enclosing to alert the permitting authority.Note that not all
applicants are required to provide attachments.
Column 1 Column 2
21 Section 1:Basic Application
a Information for all applicants. ❑ w/variance request(s) ❑ w/additional attachments
E
CO
:g ® Section 2:Additional ® w/topographic ma co InformationP ® w/process flow diagram
® w/additional attachments
1 0 Section 3:Information on ® w/Table A ® w/Table D
r Effluent Discharges ® w/Table B
® w!Table E
73
es
® w/Table C 0 w/additional attachments
re v Section 4:Industrial ❑ WI SIU and NSCIU attachments ® w/Table F
►=� Discharges and Hazardous
Wastes 0 w/additional attachments
Section 5:Combined Sewer ❑ w/CSO ma
❑ Overflows P Clw/additional attachments
❑ w/CSO system diagram
Section 6:Checklist and
® Certification Statement 0 w/attachments
6-2 Provide the following certification.(See instructions to determine the appropriate person to sign the application.)
Certification Statement
1 certify under penalty of law that this document and all attachments were prepared under my direction or supervision in
accordance with a system designed to assure that qualified personnel properly gather and evaluate the information
submitted.Based on my inquiry of the person or persons who manage the system,or those persons directly responsible for
gathering the information,the information submitted is,to the best of my knowledge and belief,true,accurate,and complete.
l am aware that there are significant penalties for submitting false information,including the possibility of fine and
imprisonment for knowing violations.
Name(print or type first and last name) f Official title
Signature Per izL1°�'"l /�"Irh rr •
Date signed
� j� I xC
641 Gii IS 1 zY
EPA Form 3510-2A
Page 12

EPA Identification Number NPDES Permit Number Facility Name Oudall Number OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of T on 001
TABLE A.EFFLUENT PARAMETERS FOR ALL POTWS
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of Methods (include
Value Units Value Units Samples units)
Biochemical oxygen demand 2 mglL DML
®BOD5 or❑ 30 mg/L 4.1 mg/L 709 SM 5210 B 0 MDL
CBOD5(report one)
ML
Fecal coliform
792 #/IOOmL 20 #/100mL 709 SM 9222 D 1#100 ml D MDL
"'Actual flow rate 2.944 Mgd 0.298 Mgd 1,645
, .
pH(minimum) 6.1
pH(maximum) 7.6
Temperature(winter) Winter Max=19 Degrees Centrigrade Degrees Centrigrade
Winter Min=6 11.3 448
Temperature(summer) Summer Max=26 Degrees Cenlrigrade Degrees Centrigrade
Summer Min=13 21.0 644
O
Total suspended solids(TSS) 2.5 m M
30 mg/L 5.4 mg/L 709 SM 2540 D 9lL 0DL
1 Sampling shall be conducted according to sufficiently sensitive test procedures(i.e.,methods)approved under 40 CFR 136 for the analysis of pollutants or pollutant parameters or
required under 40 CFR Chapter I,Subchapter N or 0.See instructions and 40 CFR 122.21(e)(3).
***The form stated Design Flow Rate.Text was modified to say actual flow rate
EPA Form 3510-2A Page 13

This page intentionally left blank.

EPA Identification Number NPDES Permit Number Facility Name Outfall Number OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon 001
TABLE B.EFFLUENT PARAMETERS FOR ALL POTWS WITH A FLOW EQUAL TO OR GREATER THAN 0.1 MGD
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of Methods (include
Value Units Value Units Samples units)
Ammonia(as N) ❑ML
14 mg/I 0.49 mg/1 709 SM4500 NH3 D O.1mgIL ❑MDL
Chlorine OML
(total residual,TRC)2 49 pg/I 35 Ng/1 712 4500 CL G 20 ugtL 0 MDL
OML
Dissolved oxygen 10.6 mg/I 6.95 mg/11,023 4500—O—G 0.1 mglL 0 MDL
Nitrate/nitrite"From Pollution Scan" OML
2.77 mg/1 1.72 mg/I 4 SM4500—NO3 H 02 mglL ❑MDL
OML
Kjeldahl nitrogen
5.66 mg/I 3.24 mg/I 21 351.2 0.1 mglL 0 MDL
Oil and grease OML
5.15 mg/1 5.04 mg/1 4 EPA 1664 B 5 mglL 0 MDL
Phosphorus OM
2.00 mg/1 0.97 mg/1 24 200.7 0.04 mg/L 0 MDL
OML
Total dissolved solids
340 mg/1 240 mg/1 4 SM 2540 C 20 mglL 0 MDL
1 Sampling shall be conducted according to sufficiently sensitive test procedures(i.e.,methods)approved under 40 CFR 136 for the analysis of pollutants or pollutant parameters or
required under 40 CFR Chapter I,Subchapter N or 0.See instructions and 40 CFR 122.21(e)(3).
2 Facilities that do not use chlorine for disinfection,do not use chlorine elsewhere in the treatment process,and have no reasonable potential to discharge chlorine in their effluent are not
required to report data for chlorine.
EPA Form 3510-2A Page 15

This page intentionally left blank.

EPA Identification Number NPDES Permit Facility Name Outfall Number OMB No.2040-0004
Number Expires 07/31/2026
Town of Tryon 001
NC0021601
TABLE C.EFFLUENT PARAMETERS FOR SELECTED POTWS
Maximum Daily Discharge Average Daily Discharge Pollutant Analytical ML or MDL
Number Methods (include units)
Value Units Value Units of
Samples
Metals,Cyanide,and Total Phenols
owHardness(as CaCO3) 39.0 mg/L 28.6 mg/L 32 SM 2340 C 1 mglL IS MDL
Antimony,total recoverable OML
<0.0050 mg/L ND mg/L 4 200.8 0.005mgIL [SI MDL
Arsenic, OML
total recoverable 0.010 mg/L 0.0025 mg/L 9 200.8 0.01 mglL 0l MDL
Beryllium,total recoverable 0.001 m ®MDL
<0.001 mg/L ND mg/L 4 200.8 9R
Cadmium,total recoverable OML
0.001 mg/L 0.001 mg/L 7 200.8 0.001 mglL 0l MDL
Chromium,total recoverable OML
0.0050 mg/L 0.0020 mg/L 6 200.8 0.005 mglL ❑MDL
Copper,total recoverable 1 u IL O MDL
15.0 pg/L 4.64 pg/L 52 200.8
Lead,total recoverable
OML
5 pg/L 1.52 PO- 8 200.8 5141 ISI MDL
Mercury,total recoverable o5 nglL OmL
MDL
20 ng/L 5.83 ng/L 3 1631 E .
Nickel,total recoverable 0.010 mg/L 0.0025 mg/L 9 200.8 0.01 mg/L ®MDL
Selenium,total recoverable OML
10 pg/L 1.4 pg/L 12 200.8 10 pg!L 0l MDL
Silver,total recoverable OML
0.005 mg/L ND mg/L 4 200.8 0.005mg1L 2]MDL
Thallium,total recoverable OML
<0.001 mg/L ND mg/L 4 200.8 0.001 mgIL ®MDL
Zinc,total recoverable OML
0.037 mg/I 0.025 mg/L 13 200.8 0.01 mglL 0l MDL
0 ML
Cyanide 0.005 mg/L 0.001 mg/L 8 SM4500CN E 0.005 mgIL ®MDL
Total phenolic compounds 0.o05 mglL ❑MDL
OML
<0.005 mg/L ND mg/L 4 420.1
Volatile Organic Compounds
Acrolein OML
5 pg/L ND WI- 4 EPA 624-8260B 55uuglL ❑MDL
See footnote
EPA Form 3510-2A Page 17
I

❑ML
Acrylonitrile <5 624 5ugIL 0 MDL
pg/L ND pg/L 4 EPA -8260B
❑ML
Benzene <0.5 pg/L ND pg/L 4 EPA 624-8260B 5 ug!L ❑MDL
❑ML
Bromoform <0.5 Ng/L ND pg/L 4 EPA 624-8260B 2 uglL ❑MDL
EPA Form 3510-2A Page 18

EPA Identification Number NPDES Permit Number Facility Name Outfall Number OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon 001
TABLE C.EFFLUENT PARAMETERS FOR SELECTED POTWS
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of Method' (include units)
Value Units Value Units Samples
Carbon tetrachloride OML
<0.5 pg/L ND pg/L ! 2 uglL ❑MDL
EPA 624-8260B
Chlorobenzene OML
<0.5 pg/L ND pg/L 4 EPA 624-8260B 2 ug/L ❑MDL
Chlorodibromomethane OML
<0.5 pg/L ND pg/L 4 EPA 624-8260B 2 ug/L 0 MDL
Chloroethane OML
<0.5 pg/L ND pg/L 4 EPA 624-8260B 2 ug/L ❑MDL
2-chloroeth vin I ether OML
y <0.5 pg/L ND pg/L 4 EPA 624-8260B 5 uglL ❑MDL
Chloroform OML
1.42 pg/L 0.36 pg/L 4 EPA 624-8260B 2 ug/L 0 MDL
OML
Dichlorobromomethane <0.5 pg/L ND pg/L 4 EPA 624-8260B 2 uglL ❑MDL
OML
1,1-di ChI0r0ethane <0.5 pg/L ND pg/L 4 EPA 624-8260B 2 uglL ❑MDL
1,2-dichloroethane OML
<0.5 pg/L ND Pa EPA 624-8260B 2 uglL 0 MDL
O
trans-1,2-dichloroethylene 2 u IL ❑MDL
<0.5 pg/L ND pg/L 4 EPA 624-8260B g
1,1-dichloroethylene OM
<05 pg/L ND pg/L 4 EPA 624-8260B 2 uglL 0 MDL
OML
1,2-dichloropropane 2 uglL ❑MDL
<0.5 pg/L ND pg/L 4 EPA 624-8260B
OML
1,3-dichloropropylene <0.5 pg/L ND pg/L 4 EPA 624-8260B 2 uglL ❑MDL
Ethylbenzene OML
<0.5 pg/L ND pg/L 4 EPA 624-8260B 2 uglL 0 MDL
Methyl bromide 2 u R O MDL
<0.5 pg/L ND pg/L 4 EPA 624-8260B g
Methyl chloride 2 u IL U MDL
<0.5 pg/L ND pg/L 4 EPA 624-8260B g
OML
Methylene chloride 2 u IL 0 MDL
<0.5 pg/L ND pg/L 4 EPA 624-8260B g
1,1,2,2-tetrachloroethane OML
<0.5 pg/L ND pg/L 4 EPA 624-8260BOML
2 ugll ❑MDL
Tetrachloroethylene 2 u ❑MDL
<0.5 pg/L ND pg/L 4 EPA 624-8260B
EPA Fomi 3510-2A Page 18

❑ML
Toluene 0.5 pg/L ND pg/L 4 EPA 624-8260B 2 uglL ®MDL
❑ML
1,1,1-trichlorcethane 0.5 pg/L ND pg/L 4 EPA 624-8260B 2 uglL ®MDL
OW
1,1,2-trichlorcethane <0.5 pg/L ND pg/L 4 EPA 624-8260B 2 ughL Z MDL
EPA Form 3510-2A Page 19

EPA Identification Number NPDES Permit Number Facility Name Outfall Number OMB No.2040-0004
NC0021601 Town of Tryon 001 Expires 07/31/2026
TABLE C.EFFLUENT PARAMETERS FOR SELECTED POTWS
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of Methods (include units)
Value Units Value Units Samples
Trichloroethylene <0.5 Ng L ND pg/L 4 EPA 624-8260B OML
2 ugR 00 MDL
Vinyl chloride L
2 ug!L ®MMDL
<0.5 pg/L ND pg/L 4 EPA624-8260B
Acid-Extractable Compounds
p-chloro-m-cresol ❑ML
<0.5 pg/L ND Pa4 EPA 625/8270D 5 uglL 0 MDL
2-chlorophenol 5 u L O MDL
<0.5 pg/L ND Pa EPA 625/8270D
OML
2,4-dichlorophenol 5 u L OMMDL
<0.5 pg/L ND pg/L 4 EPA 625/8270D
•
2,4-dimethylphenol s u ID MDL
<0.5 pg/L ND ug/L 4 EPA 625/8270D
OML
4,6-dinitro-o-cresol <0.5 pg/L ND Ng I 4 EPA 625/8270D 5uglL 0 MDL
2,4-dinitrophenol 10 u IL OML
MDL
<23.8 pg/L ND pg/L 4 EPA 625/8270D g
2-nitrophenol 5 u IL ❑MDL
<23.8 pg/L ND pg/L 4 OML
EPA 625/8270D g
4-nitrophenol s u L ❑MDL
<23.8 pg/L ND Pa 4 OML
EPA 625/8270D
Pentachlorophenol <23.8 pg/L ND pg/L 4 EPA 625/8270D 10 u oMDL
Phenol ❑ML
<4.76 pg/L ND Ug L 4 EPA 625/8270D 5 uglL ❑MDL
2,4,6-trichlorophenol 5 u lL 0 MDL
<4.76 pg/L ND pg/L 4 OM
EPA 625/8270D g
Base-Neutral Compounds
Acenaphthene <4.76 pg/L ND ug L 4 EPA 625/8270D 5 ug/L o MDL
L
Acenaphthylene 5 u OMMDL
<4.76 pg/L ND Pa EPA 625/8270D
Anthracene <4.76 pg/L ND Pa 4 L
EPA 625/8270D 5 uglL 0MMDL
Benzidine ❑ML
<9.52 pg/L ND Pa EPA 625/8270D 80 uglL ❑MDL
Benzo(a)anthracene OML
5u L O MDL
<4.76 Pa ND pg/L 4 EPA 625/8270D
EPA Form 3510-2A Page 19

L
Benzo(a)pyrene <4.76 pg/L ND pg/L 4 EPA 625/8270D 5 uglL OMMDL
O
3,4-benzoFluoranthene <4.76 pg/L ND pg/L 4 EPA 625/8270D 5 uglL O MDL
EPA Form 3510-2A Page 20

EPA Identification Number NPDES Permit Number Facility Name Outfall Number OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon 001
TABLE C.EFFLUENT PARAMETERS FOR SELECTED POTWS
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of Method' (include
Value Units Value Units Samples units)
OML
Benzo(ghi)perylene <4.76 pg/L ND pg/L 4 EPA 625/8270D 5 uglL ❑MDL
OML
Benzo(k)fluoranthene 5 ugIL ❑MDL
<4.76 pg/L ND Pe- 4 EPA 625/8270D
Bis(2-chloroethoxy)methane 5 uglL OML
MDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
Bis(2-chloroethyl)ether 5 u L OML
MDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
OML
Bis(2-chloroisopropyl)ether 5 ugIL 0 MDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
OML
Bis(2-ethylhexyl)phthalate 10 uglL ❑MDL
20 pg/L 7.48 pg/L 17 EPA 625/8270D
" in DMRs)
OL
4-bromophenyl phenyl ether s u UMMDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
O
Butyl benzyl phthalate 5 u O MDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
2-chloronaphthalene 5 uglL OML
MDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
❑ML
4-chlor hen I phenyl oP Y P Y 5 uglL ❑MDL
<4.76pg/L ND /L. 4 EPA 625/8270D
pB
Chrysene ❑ML
<4.76 pg/L ND pg/L 4 EPA 625/8270D 5 uglL ❑MDL
O
di-n-butyl phthalate 5 ugIL OMDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
di-n-octyl phthalate <4.76 pg/L ND pg/L 4 EPA 625/8270D 5 ug!L OML
MDL
Dibenzo(a,h)anthracene 5 uglL o MDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
O
1,2-dichlorobenzene <0.5 pg/L ND pg/L 4 EPA625/8270D 5ugIL 0MDL
1,3-dichlorobenzene <0.5 pg/L ND pg/L 4 EPA 625/8270D 5 ug!L 0MDL
1,4-dichlorobenzene <0.5 pg/L ND pg/L 4 EPA 625/8270D 5 uglL 0 MDL
OML
3,3-dichlorobenzidine 10.2 pg/L 2.55 pg/L 4 EPA 625/8270DO
5 uglL ❑MDL
Diethyl phthalate 5 ugIL OMDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
Dimethyl phthalate 5 uglL 0ML
MDL
<4.76 pg/L ND pg/L 4 EPA 625/870D
Page 20

2,4dinitrotoluene ❑ML
<4.76 Ng L ND Ng L 4 EPA 625/8270D 5 uglL ❑MDL
• ❑ML
2,6-dinitrotoluene <4.76 Ng/L ND pg/L 4 EPA 625/8270D 5 uglL ❑MDL
EPA Form 3510-2A Page 21

EPA Identification Number NPDES Permit Number Facility Name Outfall Number OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon 001
TABLE C.EFFLUENT PARAMETERS FOR SELECTED POTWS
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of Method' (include
Value Units Value Units Samples units)
O
1.2-diphenylhydrazine 5 u L ❑MDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
M
Fluoranthene <4 76 pg/L ND pg/L 4 EPA 625/8270D 5 uglL ❑MDL
Fluorene <4.76 pg/L ND pg/L 4 EPA 625/8270D 5 ugIL 0 MDL
HexachlorobenzeneO
<4.76 pg/L ND pg/L 4 EPA 625/8270D 5 uglL ❑MDL
OML
Hexachlorobutadiene <4.76 pg/L ND pg/L 4 EPA 625/8270D 5 ug/L ❑MDL
Hexachlorocydo-pentadiene 5 uglL ❑MMDL
<23.8 pg/L ND pg/L 4 EPA 625/8270D
OW
Hexachloroethane <4.76 pg/L ND pg/L 4 EPA 625/8270D 5 ugIL 0 MDL
O
Indeno(1,2,3cd)pyrene <4.76 pg/L ND pg/L 4 EPA 625/8270D 5ugh. ❑MDL
OML
lsophorone 5 u ❑MDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
Naphthalene 5 uglL ❑MMDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
ugh. L
Nitrobenzene <4.76 pg/L ND pg/L 4 EPA 625/8270D 5 ug ❑MDL
OL
N-nitrosodi-n-propylamine SugIL ❑MMDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
O
N-nitrosodimethylamine
<4.76 pg/L ND pg/L 4 EPA 625/8270D 5 uglL ❑MDL
O
N-nitrosodiphenylamine S u L MDL
<4.76 pg/L ND pg/L 4 EPA 625/8270D
OML
Phenanthrene <4.76 pg/L ND pg/L 4 EPA 625/8270D 5 uglL ❑MDL
❑ML
Pyrene <4.76 pg/L ND pg/L 4 EPA 625/8270D 5 ugh. ❑MDL
OML
1,2,4 trichlorobenzene <0.5 pg/L ND pg/L 4 EPA 625/8270D 2 uglL 0 MDL
Sampling shall be conducted according to sufficiently sensitive test procedures(i.e.,methods)approved under 40 CFR 136 for the analysis of pollutants or pollutant parameters or
required under 40 CFR Chapter I,Subchapter N or 0.See instructions and 40 CFR 122.21(e)(3).
***If neither ML or MDL was selected,the Quantitation Level shown in the annual Pollution Scans was reported in this column.
EPA Form 3510-2A Page 21

This page intentionally left blank.

EPA Identification Number NPDES Permit Number Facility Name Outfan Number OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon 001
TABLE D.ADDITIONAL POLLUTANTS AS REQUIRED BY NPDES PERMITTING AUTHORITY
Pollutant Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
(list) Value Units Value Units Number of Method, (include units)
Samples
❑ No additional sampling is required by NPDES permitting authority.
• onia(NH3 as N)April 1— ❑ML
•ctober 31) C0610 SM4500 NH3 D 0.1mg1L ❑MDL
**Whole year is listed in Table 13 nrg L 0 40 mg 1. 418
is**
• onia(NH3 as N) ❑ML
I ovember 1 to March 31) SM4500 NH3 D 0.1mglL ❑MDL
0610 14 mg L 0.63 mg/L 286
**Whole year is listed in Table
I:**
otal Nitrogen,mg/L ❑ML
NO2+NO3+TKN) Effluent 18 4500-N 0 MDL
0600 5.7 mg/L 3.24 mg/L
hloride-Effluent 00940 0 ML
83 40.7 46 4500-Cl ❑MDL
mg/L mg/L
u issolved Oxygen 00300
❑ML
pstream 4500—0—G 0.1 mglL 0 MDL
13.8 mg/L 8.4 mg/L 430
'Dissolved Oxygen 00300
OM
i•ownstream 430 4500—0—G 0.1 mg/L ❑MDL
69 mg/L 8.7 mg/L
emperature Upstream 00010
26 °C 18.4 °C 430
emperature Downstream
10010 24 °C 18 °C 430
onductivity Upstream, 0 ML
os/cm 00094 72 pmhos/cm 42.9 pmhos/cm 430 2510 Conductivity ❑MDL
onductivity Downstream, 0 ML
os/cm 00094 124 pmhos/cm 51.5 pmhos/cm 430 2510 Conductivity ❑MDL
otal Hardness[as CaCO3], ❑ML
pstream, mg/L 00900 32 mg/L 18.1 mg/L 14 SM 2340 C 1 mgIL El MDL
1 Sampling shall be conducted according to sufficiently sensitive test procedures(i.e.,methods)approved under 40 CFR 136 for the analysis of pollutants or pollutant parameters or required
under 40 CFR Chapter I,Subchapter N or 0.See instructions and 40 CFR 122.21(e)(3).
EPA Form 3510-2A Page 23

This page intentionally left blank.

EPA Identification Number NPDES Permit Number Facility Name Outfall Number OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon 001
TABLE E.EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample.Copy the table to report additional test results.
Test Information
Test Number ETS,Inc. 19249 Test Number ETS,Inc. 18976 Test Number ETS,Inc. 17991
Test species
Age at initiation of test
Outfall number
Date sample collected
eriodaphnia dubia EPA 1002.0 Ceriodaphnia dubia EPA 1002.0 Ceriodaphnia dubia EPA 1002.0
24-hours old <24-hours old <24-hours old
limil
o1 001 001
12/11/2023 9/4/23 08:00-9/5/23 08:00 6/12/23 08:00-6/13 08:00
Date test started 12/13/2023 9/6/2023 6/14/2023
Duration -days 7-days 7-days
Toxicity Test Methods
Test method number EPA-821-R-02-013; Method 1002.0 EPA-821-R-02-013; Method 1002.0 EPA-821-R-02-013; Method 1002.0
Manual title Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms,EPA-821-R-02-013
Edition number and year of publication Fourth Edition,October 2002 ; NC Modification-December 2010,Version 3.0
Page number(s) I-335
Sample Type
Check one: 0 Grab ❑ Grab 0 Grab
®24-hour composite ®24-hour composite ®24-hour composite
Sample Location
Check one: 0 Before disinfection ❑ Before disinfection 0 Before disinfection
0 After disinfection ❑After disinfection 0 After disinfection
®After dechlorination ®After dechlorination ®After dechlorination
Point in Treatment Process
Describe the point in the treatment process Effluent Outfa11001,after all treatment Effluent Outfall 001,after all treatment Effluent Outfall 001,after all treatment
at which the sample was collected for each processes processes processes
test.
Toxicity Type
Indicate for each test whether the test was ❑Acute ❑Acute ❑Acute
performed to assess acute or chronic
toxicity,or both.(Check one response.) ®Chronic 0 Chronic ®Chronic
❑Both 0 Both 0 Both
EPA Form 3510-2A Page 25

EPA Identification Number NPDES Permit Number Facility Name Outfa11 Number OMB No 2040-0004
Expires 0713112026
NC0021601 Town of Tryon 001
TABLE E.EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample.Copy the table to report additional test results.
Test Number ETS,Inc. 19249 Test Number EIS,Inc. 18976 Test Number ETS,Inc. 17991
Test Type
Indicate the type of test performed.(Check ❑ Static ❑ Static 0 Static
one response.)
® Static-renewal Z Static-renewal 0 Static-renewal
❑ Flow-through 0 Flow-through 0 Flow-through
Source of Dilution Water
Indicate the source of dilution water.(Check ® Laboratory water ®Laboratory water ®Laboratory water
one response.)
❑ Receiving water 0 Receiving water 0 Receiving water
If laboratory water,specify type. Moderately hard(MHSW) Moderately hard(MHSW) Moderately hard(MHSW)
If receiving water,specify source.
Type of Dilution Water
Indicate the type of dilution water.If salt ®Fresh water Z Fresh water 0 Fresh water
water,specify"natural"or type of artificial
sea salts or brine used.
0 Salt water(specify) ❑ Salt water(specify) ❑ Salt water(specify)
Percentage Effluent Used
Specify the percentage effluent used for all Effluent: 37% Effluent: 37% Effluent: 37%
concentrations in the test series.
Parameters Tested
Check the parameters tested. ®pH 0 Ammonia ®pH ❑Ammonia ®pH 0 Ammonia
❑ Salinity ®Dissolved oxygen 0 Salinity ►5 Dissolved oxygen 0 Salinity ❑Dissolved oxygen
Z Temperature ®Temperature Z Temperature
Acute Test Results
Percent survival in 100%effluent % % %
LC5o
95%confidence interval % % %
Control percent survival % % %
EPA Form 3510-2A Page 26

EPA Identification Number NPDES Permit Number Facility Name Outfall Number OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon 001
TABLE E.EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample.Copy the table to report additional test results.
ETS,Inc.Test Number 19249 ETS,Inc.Test Number 18976 ETS,Inc.Test Number 17991
Acute Test Results Continued
Other(describe)
Chronic Test Results
NOEC % % %
IC2s See control charts: -1.095 g/L NaCI % See control charts: -1.095 g/L NaCI % See control charts: -1.09 g/L NaCI %
Control percent survival 100% 100% 100%
Other(describe)
Quality ControllQuality Assurance
Is reference toxicant data available? ❑ Yes ❑ No 0 Yes ❑ No 0 Yes ❑ No
Was reference toxicant test within
acceptable bounds? ®Yes ❑ No ®Yes 0 No ®Yes ❑ No
What date was reference toxicant test run 12/13/2023 9/6/2023 6/14/2023
(MMIDDIYYYY)?
Other(describe)
EPA Form 3510-2A Page 25

EPA Identification Number NPDES Permit Number Facility Name Outfall Number OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon 001
TABLE E.EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample.Copy the table to report additional test results.
Test Information
Test Number ETS,Inc.17503 Test Number ETS,Inc. 17229 Test Number ETS,Inc.16998
Test species eriodaphnia dubia EPA 1002.0 Ceriodaphnia dubia EPA 1002.0 Fathead Minnow,Pimephales promelas EPA
1000.0
Age at initiation of test 24-hours old <24-hours old <24-hours old
Outfall number 01 001 001
Date sample collected /13/23 08:00—3/14/23 08:00 12/12/22 08:00—12/13/22 08:00 6/5/22 08:00—6/6/22 08:00
Date test started /15/2023 12/14/2022 6/7/2022
Duration -days 7-days 7-days
Toxicity Test Methods
Test method number EPA-821-R-02-013; Method 1002.0 EPA-821-R-02-013; Method 1002.0 EPA-821-R-02-013; Method 1000.0
Manual title Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms,EPA-821-R-02-013
Edition number and year of publication Fourth Edition,October 2002 ; NC Modification—December 2010,Version 3.0
Page number(s) I-335
Sample Type
Check one: 0 Grab 0 Grab 0 Grab
®24-hour composite ®24-hour composite ®24-hour composite
Sample Location
Check one: ❑ Before disinfection ❑ Before disinfection 0 Before disinfection
❑After disinfection ❑After disinfection ❑After disinfection
®After dechlorination ®After dechlorination ®After dechlorination
Point in Treatment Process
Describe the point in the treatment process Effluent Weir before outfall Effluent Weir before outfall Effluent Weir before outfall
at which the sample was collected for each
test.
Toxicity Type
Indicate for each test whether the test was 0 Acute 0 Acute ❑Acute
performed to assess acute or chronic
toxicity,or both.(Check one response.) ®Chronic ®Chronic ®Chronic
❑Both 0 Both 0 Both
EPA Form 3510-2A Page 26

EPA Identification Number NPDES Permit Number Facility Name Outfall Number OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon 001
TABLE E.EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample.Copy the table to report additional test results.
Test Number ETS,Inc. 17753 Test Number ETS,Inc. 17503 Test Number ETS,Inc. 16998
Test Type
Indicate the type of test performed.(Check 0 Static 0 Static ❑Static
one response.)
1 Static-renewal ®Static-renewal ®Static-renewal
❑ Flow-through ❑ Flow-through ❑ Flow-through
Source of Dilution Water
Indicate the source of dilution water.(check ® Laboratory water—Moderately hard ® Laboratory water—Moderately hard ® Laboratory water—Moderately hard
one response.) (MHSW) (MHSW) (MHSW)
Receiving water Receiving water Receiving water
If laboratory water,specify type. Moderately hard(MHSW) Moderately hard(MHSW) Moderately hard(MHSW)
If receiving water,specify source.
Type of Dilution Water
Indicate the type of dilution water.If salt ® Fresh water ®Fresh water 0 Fresh water
water,specify"natural"or type of artificial
sea salts or brine used. ❑ Salt water(specify) ❑ Salt water(specify) 0 Salt water(specify)
Percentage Effluent Used
Specify the percentage effluent used for all Effluent: 37% Effluent: 37% Effluent: 9.25%; 18.5%; 37%,74%,100%
concentrations in the test series.
-
Parameters Tested
Check the parameters tested. ®pH ❑Ammonia ®pH ❑Ammonia 0 pH 0 Ammonia
❑ Salinity Z Dissolved oxygen 0 Salinity ® Dissolved oxygen ❑ Salinity ❑ Dissolved oxygen
®Temperature ®Temperature ®Temperature
Acute Test Results
Percent survival in 100%effluent % % %
LCso
95%confidence interval % a/, %
Control percent survival % % %
EPA Forth 3510-2A Page 26

EPA Identification Number NPDES Permit Number Facility Name Oudall Number OMB No.2040-0004
Expires 07/31/2926
NC0021601 Town of Tryon 001
TABLE E.EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample.Copy the table to report additional test results.
Test Number ETS,Inc. 17753 Test Number ETS,Inc. 17503 Test Number ETS,Inc. 16998
Acute Test Results Continued
Other(descnbe)
Chronic Test Results
NOEC % % 100%
IC25 See control charts: -1.095 g/L NaCI % See control charts: -1.10 g/L NaCI % 100%
Control percent survival 100% 100% 100%
Other(describe) LOEC->100%
LOEC&ChV Ch V->100%
Quality ControllQuality Assurance
Is reference toxicant data available? ❑Yes ❑ No ❑Yes ❑ No ❑Yes ❑ No
•
Was reference toxicant test within
acceptable bounds? ®Yes ❑ No ®Yes ❑ No ®Yes ❑ No
What date was reference toxicant test run 3/15/2023 12/14/2022 6/8/2022
(MM/DD/YYYY)?
Other(describe)
EPA Form 3510-2A Page 27

This page intentionally left blank.

EPA Identification Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
TABLE F.INDUSTRIAL DISCHARGE INFORMATION
Response space is provided for three SIUs.Copy the table to report information for additional SIUs.
SIU 001 SIU_ SIU
Name of SIU
Tryon Finishing Corporation
Mailing address(street or P.O.box)
250 Screvens Rd.
City,state,and ZIP code
Tryon,NC 28782
Describe all industrial processes that affect or
contribute to the discharge.
Textile,Bleaching and Dyes,Synthetic and
Natural Yarn
List the principal products and raw materials that Raw Materials:Cotton and polyester/cotton
affect or contribute to the SIU's discharge. cloth;Dyes,Bleach,Salt,Caustic,Hydrogen
Peroxide,acetic acid,softeners
Products :Printed cloth
Indicate the average daily volume of wastewater
36,000 gpd gpd gpd
by
discharged the SIU.
How much of the average daily volume is
gpd
attributable to process flow? 30,000gpd gpd
How much of the average daily volume is
attributable to non-process flow? 6,000 gpd gpd gpd
Is the SIU subject to local limits?
®Yes ❑ No ❑ Yes ❑ No ❑Yes ❑ No
Is the SIU subject to categorical standards?
®Yes ❑ No ❑Yes ❑ No 0 Yes ❑ No
Check 40 CFR#410"Textile Mills"
EPA Form 3510-2A Page 29

EPA Identficadon Number NPDES Permit Number Facility Name OMB No.2040-0004
Expires 07/31/2026
NC0021601 Town of Tryon
TABLE F.INDUSTRIAL DISCHARGE INFORMATION
Response space is provided for three SIUs.Copy the table to report information for additional SIUs.
SIU 001 SIU_ SIU
Under what categories and subcategories is the 40 CFR Part 410
SIU subject?
Has the POTW experienced problems(e.g.,
upsets,pass-through interferences)in the past ❑Yes ®No 0 Yes ❑ No ❑Yes ❑No
4.5 years that are attributable to the SW?
If yes,describe. SIU has been provided with NOVs,but these
have not caused POTW effluent limit
concerns.
Click to go back to the beginning of Form
EPA Form 3510-2A Page 30

Annual monitoring and Pollutant Scan .
Month June
Permit No. NC002160 Year 2019
Outfall 001
ORC Bradley
Facility Name Tryon Wastewater Plant Phone Deborah828) 82 5626
Date of Sampling 6/11/19
Analytical Laboratory ETS, INC.
Analytical Quantitation Sample Units of
Parameter Sample A
Parameter Code Type Method Level Result Measurement
0.1 0.38 mg/L
C0610 Composite SM 4500 NH3 0 38 ug/L
Ammonia(as N) Gmb _4500 Ci G 20 _-_ --
Chlorine(total residual,TRC) 50060 _�T- - 3 6^ mg/L -
00300 Grab 4500-O-G 0.1 _-•__----_. _
Dissolved Oxygen _. - -- 0 2 6.6 mg/L
00630 Composite SM4500-NO3 H
Nitrite plus Nitrate Total(as N) .1.95--
Oil and Greasemg/L
Total Kjeldahl Nitrogen - 00625 - _Composite -- 3-._- _351.2 0.1 1 ---•-__<5 0 mg/L
00556 Grab EPA 1664A 5 -
_ ----- -- _- 200.7 0.1 1.5 mg/L
Total Phosphorus - C0665 Composite-_ _ 140 -
Total Dissolved Solids mg/
70295 Composite SM2540 C 10 -�_
__-.-_._-.__--- - -----------.
Hardness`� 00900 Composite _SM2340- C 1 28 mg/L
Metals(total recoverable),cyanide and total phenols 0.005 <0.005 mg/L
Antimony 01097 Composite 200.8- 0.01 <0.010 mg/L_
g/L
Arsenic 01002Ny Composite --200.8 -._-,r-_-•_._-_..._._..---
Beryllium ,01012- Composite - 200.8 _ _ __
Cadmium 01 mg/L
01027 Composite y 200.8- 0.001
0.005 <0.005 mg/L
Chromium J 01034 Composite 200.8
01042 Composite 200.8 0.001 0.008 mg/L
Copper 200.8 0.005 <0.005 mg/L
Lead 01051 Composite -__, 1 82 ng/L
COMER Composite 245.1 0.5 -
Mercury(Method 1631E) _ -_ --_----a- 0.01 <0.010 mg/L
Nickel 01067 _Composite 200.8 <0.010 T mg/L
01147 Composite 200.8 0.01 __
Selenium __ .--__--.-----------•--_.-~- `_------__ _ --<0-005 mg/L
Silver -� 01077 Composite 200.8 0.005 . mg/L
01059 Composite 200.8 0.001 - _<0.0005
Thallium --200.8 0.01 0.01 0.04 mg/L
Zinc - _. _ Composite <0.005 mg/L
00720 Grab SM4500CN E 0.005
Cyanide ------- !420.1 0.005 <0.005 mg/L
Total phenolic compounds
32730 Grab
Volatile organic compounds 5 ND ug/L
34210 Grab EPA 624-8260B ___ ND
Acrolein --- EPA 624-8260B 5
34215 Grab ----- ND ug/L
Acrylonitrile _ -_..____ __-- 2
34030 Grab EPA 624-8260B ----
32104 Grab EPA 624-8260B ND
IIIIEMIIIIII
--
Benzene _- ___ - - --_-__ __. _ 2
ND ug/L
--Bromoform _ -_._.--------- EPA 62a 82606 2
�"-� 32102 Grab
Carbon Tetrachloride __ __-_ - EPA 62a a__ 2 ND
34301 Grab ND ug/L
Chlo______ ene _____ _..---.-- .... ~Epq sea-szso6 2
34306 Grab D
Chlorodibromomethane __�______. _---- 2
Chloroethane �85811 - Grab.___.EPA 624 8260E NDND 11110111111
-
- r 34576 Grab EPA 624-8260B 5 2 chloroeth111.
yl vinyl ether - EPA sz4 82so6 2 ND ug/L
32106 Grab '--
Dichlorobromomethan MEM
ND
Chloroform Grab EPA 624-82606 2
~-l`- 32101
e EPA s-- 2a e2s06 2 ND -
34496 Grab - - ND
1,1-dichloroethane21111
EPA 6q e2so6 2 -
11
32103 Grab --- ND ug/L
1,2-dichloroethane _------------ 2 _.`�-
-'" 34546 Grab EPA 624 82606 _
Trans-1,2-dichloroethylene
Page 1
Form - DMR- PPA-1

Annual nnonitorIng and Pollutant Scan
Permit No. NC002160 Month lune
Outfall 001 Year 2019
Parameter Sample Analytical Quantitation Sample Units of
Parameter Code Type Method Level Result Measurement
1,1-dichloroethylene 34501 Grab EPA 624-8260B 2— ND ug/L
1,2-dichloropropane 34541 Grab EPA 624-8260B 2 ND ug/L
1,3-dichloropropylene 77163 Grab EPA B24-8280B 2 ND ug/L -
Ethylbenzene 34371 -_ Grab EPA 6248260E 2 ND ug/L
Methyl Bromide 34413 Grab EPA 624-8260B 2 ND ug/L
Methyl Chloride 34418 Grab EPA 624-8260B 2 ND ug/L
Methylene Chloride 34423 Grab EPA 624-8280B 2 ND ug/L
1,1,2,2-tetrachloroethane 81549 Grab EPA 624-82608 2 _ ND ug/L
Tetrachloroethylene 34475 Grab EPA 624-8260B 2 ND ug/L
Toluene__^ 34010 Grab EPA 624-8280B 2 ND ug/L
1,1,1-trichloroethane 34506 Grab EPA 624.8260E 2 ND ug/L
1,1,2-trichloroethane 34511 Grab EPA 624-82608 2 ND ug/L
Trichloroethylene 39180 Grab EPA 624-8260B 2 ___ ND ug/L
Vinyl Chloride 39175 Grab EPA 624-8280B 2 ND ug/L .
Acid-extractable compounds
P-chloro-m-creso 34452 Grab EPA 625/8270D 5 ND ug/L
2-chlorophenol 34586 Grab— EPA 625/8270D 5 ND ug/L
2,4-dichlorophenol 34601 Grab EPA 62518270D 5 - ND ug/L
2,4-dimethylphenol 34606 Grab EPA 625/82700 5 ND ug/L
4,6-dinitro-o-cresol 34657 Grab EPA 625/8270D 5 ND ug/L
2,4-dinitrophenol v 34616 T Grab EPA 625/8270D 10 ND ug/L
2-nitrophenol �34591 _ Grab EPA 625/8270D 5
ND ug/L
4-nitrophenol � ~� µ 34646 Grab EPA 625/8270D 5 ND ug/L
Pentachlorophenol _
39032 Grab EPA 625/8270D 10 _ ND ug/L
Phenol) _ 34694 Grab EPA 625/8270D 5 ND ug/L
2,4,6-trichlorophenol 34621 Grab EPA625/8270D 5 ND ug/L
Base-neutral compounds
Acenaphthene 34205 Grab EPA 625/8270D 5 ND ug/L
Acenaphthylene 34200 Grab _ EPA 62518270D 5 ND ug/L
Anthracene CO220 Grab _ EPA 625/8270D 5 ND ug/L
Benzidine 39120 Grab EPA 625/8270D 80 ND ug/L
Benzo(a)anthracene 34526_ Grab EPA 625/82700 5 — ND ug/L
Benzo(a)pyrene 34247 Grab EPA 625/8270D 5
ND ug/L
3,4 benzofluoranthene 34230 Grab EPA625/8270D 5 ND ug/L
Benzo(ghi)perylene 34521 Grab EPA 62518270D 5 ND ug/L
Benzo(k)fluoranthene 34242 Grab EPA 625/8270D 5 ND ug/L
Bis (2-chloroethoxy) methane 34278 ^Grab EPA 625/8270D 5
ND ug/L
Bis (2 chloroethyl) ether
34273 Grab EPA 625/8270D 5 ND ug/L
�_— ND ug/L
Bis (2 chloroisopropyl) ether 34283 Grab EPA 625/8270D __5 _ ND ug/L
Bis (2-ethylhexyl) phthalate 39100 ^_T Grab EPA 62518270D 10
4-bromophenyl phenyl ether 34636 Grab EPA 625/8270D 5 ND ug/L
Butyl benzyl phthalate 34292 _�Grab EPA 62518270D 5 ND ug/L
2 chloronaphthalene
34581 Grab EPA 625/82700 5 ND ug/L
_
4-chlorophenyl phenyl ether _ 34641 Grab EPA 625/8270D _ 5 ^` ND ug/L
34320 Grab EPA 625/62700 5 ND ug/L
ChryseneT� T_____ --
Page 2
Form - DMR- PPA-1

Annual Monitoring and Pollutant Scan
Month June
Permit No. NC002160 Year 2019
Outfall 001
Analyticaluantitation Sample Units of
Parameter Sample Q Level Result Measurement
Parameter
Code •Type Method
39110 Grab EPA 625/8270D __ _
Di-n-butyl phthalate •--� ND ug/L
-^-
Di-n-octy phthalateEPA 625/8270D � ND ug/L
__ 5 _
1 34596 Grab —__ ND ug/L
Dibenzo(a,h)anthracene 34556 Grab EPA 625/8270D 1,2 dichlorobenzene ND ug/L
34536 Grab EPA 625/8270D 5
_____ --
Grab
1,3 dichlorobenzene 34566 _ EPA ND ug/L
PA 625/82700 5 ND ug/L
1,4-dichlorobenzene 34571 Grab EPA 625/8270D 5 ND ug/L
3,3 dichlorobenzidine 34631 Grab EPA 625/8270D 5 ND ug/L
Diethyl phthalate 34336- e_ Grab EPA 625/8270D 5 ND ug/L
Dirnethyl phthalate 34341 Grab— EPA 625/82700 5 ND ug/L
2,4-dinitrotoluene 34611 Grab EPA 625/8270D 5 ND ug/L
2,6-dinitrotoluene CO626 Grab EPA 625/8270D 5
- EPA 625182700 5 ND ug/L
1,2 diphenylhydrazine 34346 Grab - ND ug/L
Fluorene
Fluoranthene CO376 Grab EPA 625/8270D 5 ND ug/L
34381 Grab EPA 625/8270D 5
C0700 Grab EPA 625/8270D
5 ND ug/L
Hexachlorobenzene Grab EPA 625/8270D 5 ND ug/L
39702
Hexachlorobutadiene Grab EPA 62518270D 5 ND ug/L
Hexachlorocyclo pentadiene 34386_— ------ ND ug/L
Hexachloroethane 34396 Grab EPA 62518270D 5
— ND ug/L
indeno(1,2,3-cd)pyrene 34403 Grab EPA 625/8270D 5 Isophorone ND ug/L
34408 Grab EPA 625/8270D 5
_
34696 Grab EPA 625/82700 5 ND ug/L
Naphthalene 5 ND ug/L
Nitrobenzene _ 34447 Grab EPA 625/8270D
Grab EPA 625/8270D 5 ND ug/L
N nitrosodi n propylamine 34428 ---- ND ug/L
„
N nitrosodimethylamine 34438 Grab EPA 625/8270D 5_.___- Grab EPA 625/8270D 5 ND ug/L
34433
N-nitrosodiphenylamine --- ND ug/L
Phenanthrene 34461 Grab EPA 625/82700 5 Pyrene ND ug/L
34469 Grab EPA 625/8270D 5
— -----_'�_. �_.�.2 ND ug/L
1,2,4,-tichoorobenzene
34551 Grab EPA 624 82608
n or supervision in
"I certify,under penalty of law,that this document
erso n 1 ll roachments erly gather and e a uate t e under
mformation submitted. Based on myaccordance
with a system designed to assure that qualified p p p
e
inquiry of the person or persons who managed the system,
and belief,truee r accuratesons ,and for gathering the information,
aware that there are significant
information submitted is,to the best of my knowledge
penalties for submitting false information, including the possibility of fines and imprisonment for knowing violations."
Authorized Representative name
Signature )
ii )i`t
Date
Page 3
Form- DMR- PPA-1

Annual Monitoring and Pollutant Scan
Facility Name Tryon Wastewater Plant ORC Deborah Bradley
Date of Sampling 6/8/21 Phone [phone]
Analytical Laboratory ETS, INC.
Parameter Sample Analytical Quantitation Sample Units of
Parameter Code Type Method Level Result Measurement
Ammonia (as N) C0610 Composite SM 4500 NH3 D 0.1 <0.10 mg/L
Chlorine (total residual,TRC) 50060 Grab 4500 Ci G 20 27 ug/L
Dissolved Oxygen 00300 Grab 4500-O-G 0.1 5.9 mg/L
Nitrite plus Nitrate Total(as N) 00630 Composite SM4500-NO3 H 0.2 2.44 mg/L
Total Kjeldahl Nitrogen 00625 Composite 351.2 0.1 0.98 mg/L
Oil and Grease 00556 Grab EPA 1664A 5 <5.0 mg/L
Total Phosphorus C0665 Composite 200.7 0.1 0.89 mg/L
Total Dissolved Solids 70295 Composite SM2540 C 10 140 mg/L
Hardness 00900 Composite SM2340 C 1 24 mg/L
Metals(total recoverable), cyanide and total phenols
Antimony 01097 Composite 200.8 1 0.005 <0.005 mg/L
Arsenic 01002 Composite 200.8 0.01 <0.010 mg/L
Beryllium 01012 Composite 200.8 0.001 <0.001 mg/L
Cadmium 01027 Composite 200.8 0.001 <0.001 mg/L
Chromium 01034 Composite 200.8 0.005 <0.005 mg/L
Copper 01042 Composite I 200.8 0.001 0.005 mg/L
Lead 01051 Composite 200.8 0.005 <0.005 mg/L
Mercury(Method 1631E) COMER Composite 245.1 0.5 <0.500 ng/L
Nickel 01067 Composite 200.8 0.01 <0.010 mg/L
Selenium 01147 Composite 200.8 0.01 <0.010 mg/L
Silver 01077 Composite 200.8 0.005 <0.005 mg/L
Thallium 01059 Composite 200.8 0.001 <0.0005 mg/L
Zinc 01092 Composite 200.8 0.01 0.037 mg/L
Cyanide 00720 Grab SM4500CN E 0.005 <0.005 mg/L
Total phenolic compounds 32730 Grab 420.1 0.005 <0.005 mg/L
Volatile organic compounds
Acrolein 34210 Grab EPA 624-8260B 5 ND ug/L
Acrylonitrile 34215 Grab EPA 624-82608 5 ND ug/L
Benzene 34030 Grab EPA 624-8260B 2 ND ug/L
Bromoform 32104 Grab EPA 624-8260B 2 ND ug/L
Carbon Tetrachloride 32102 Grab EPA 624-8260B 2 ND ug/L
Chlorobenzene 34301 Grab EPA 624-8260B 2 ND ug/L
Chlorodibromomethane 34306 Grab EPA 624-8260B 2 ND ug/L
Chloroethane 85811 Grab EPA 6248260E 2 ND ug/L
2-chloroethyl vinyl ether 34576 Grab EPA 624-82608 5 ND ug/L
Chloroform 32106 Grab EPA 624-8260B 2 ND ug/L
Dichlorobromomethane 32101 Grab EPA 624-826013 2 ND ug/L
1,1-dichloroethane 34496 Grab - EPA 824-8260B 2 ND ug/L
1,2-dichloroethane 32103 Grab EPA 624-8260B 2 ND ug/L
Trans-1,2-dichloroethylene 34546 Grab EPA 624-8260B 2 ND ug/L
Form - DMR- PPA-1 Page 1

Annual Monitoring and Pollutant Scan
Parameter Sample Analytical Quantitation Sample Units of
Parameter Code Type Method Level Result Measurement
1,1-dichloroethylene 34501 Grab EPA 624-8260B 2 ND ug/L
1,2-dichloropropane 34541 Grab EPA 624-8260B 2 ND ug/L
1,3-dichloropropylene 77163 Grab EPA624-8260B 2 ND ug/I,
Ethylbenzene 34371 Grab EPA 624-82808 2 ND ug/L
Methyl Bromide 34413 Grab EPA 624-82608 2 ND ug/L
Methyl Chloride 34418 Grab EPA 624-8260B 2 ND ug/L
Methylene Chloride _ 34423 , Grab EPA 624-8260B 2 ND ug/L
1,1,2,2-tetrachloroethane 81549 Grab EPA624-82608 2 ND ug/L
Tetrachloroethylene 34475 Grab EPA 624-8260B 2 ND ug/L
Toluene 34010 Grab EPA 624-8260B 2 ND ug/L
1,1,1-trichloroethane 34506 Grab EPA624-8260B 2 ND ug/L
1,1,2-trichloroethane 34511 Grab EPA 624-82608 2 ND ug/L
Trichloroethylene 39180 Grab EPA624-8260B 2 ND ug/L
Vinyl Chloride 39175 Grab EPA 624-8260B 2 ND ug/L
Acid-extractable compounds
P-chloro-m-creso 34452 Grab EPA 625/8270D 5 ND ug/L
2-chlorophenol 34586 Grab EPA 625/8270D 5 ND ug/L
2,4-dichlorophenol 34601 Grab EPA 625/8270D 5 ND ug/L
2,4-dimethylphenol 34606 Grab EPA 625/82700 5 ND ug/L
4,6-dinitro-o-cresol 34657 Grab EPA 625/8270D 5 ND ug/L
2,4-dinitrophenol 34616 Grab EPA 625/8270D 10 ND ug/L
2-nitrophenol 34591 Grab EPA 625/8270D 5 ND ug/L
4-nitrophenol 34646 Grab EPA 625/8270D 5 ND ug/L
f Pentachlorophenol 39032 Grab EPA 625/8270D 10 ND ug/L
Phenol 34694 Grab EPA 625/8270D 5 ND ug/L
2,4,6-trichlorophenol 34621 Grab EPA625/8270D 5 ND ug/L
Base-neutral compounds
Acenaphthene 34205 Grab EPA 625/8270D 5 ND ug/L
Acenaphthylene 34200 Grab EPA 625/8270D 5 ND ug/L
Anthracene CO220 Grab EPA625/8270D 5 ND ug/L
L
I Benzidine 39120 Grab EPA 625/8270D 80 ND ug/L
Benzo(a)anthracene 34526 Grab EPA 625/8270D 5 ND ug/L
Benzo(a)pyrene 34247 Grab EPA 625/8270D 5 ND ug/L
3,4 benzofluoranthene 34230 Grab EPA625/8270D 5 ND ug/L
,
Benzo(ghi)perylene 34521 Grab EPA 625/82700 5 ND ug/L
Benzo(k)fluoranthene 34242 Grab EPA625/8270D 5 ND ug/L
Bis(2-chloroethoxy)methane 34278 Grab EPA 625/8270D 5 ND ug/L
Bis (2-chloroethyl)ether 34273 Grab EPA 625/8270D 5 ND ug/L
— ^—
Bis(2-chloroisopropyl)ether 34283 Grab EPA 625/8270D 5 ND ug/L
Bis(2-ethylhexyl) phthalate 39100 Grab EPA 625/8270D 10 ND ug/L
4-bromophenyl phenyl ether 34636 Grab EPA 625/8270D 5 ND ug/L
Butyl benzyl phthalate 34292 Grab EPA 625/8270D 5 ND ug/L
2-chloronaphthalene 34581 Grab EPA 625/8270D 5 ND ug/L
4-chlorophenyl phenyl ether 34641 Grab EPA 625/8270D 5 ND ug/L
Chrysene 34320 Grab EPA625/8270D 5 ND ug/L
Form - DMR- PPA-1 Page 2

Annual Monitoring and Pollutant Scan
Parameter Sample Analytical Quantitation Sample Units of
Parameter Code Type Method Level Result Measurement
Di-n-butyl phthalate 39110 Grab EPA 825/8270D 5 ND ug/L
Di-n-octyl phthalate 34596 Grab EPA 625/82700 5 ND ug/L
Dibenzo(a,h)anthracene 34556 Grab EPA 625/8270D 5 ND ug/L
1,2-dichlorobenzene _34536 Grab EPA 825/8270D 5 ND ug/L
1,3-dichlorobenzene 34566 Grab EPA 825/8270D 5 ND _ ug/L
1,4-dichlorobenzene 34571 Grab EPA 825/8270D 5 ND ug/L
3,3-dichlorobenzidine 34631 Grab EPA825/8270D 5 ND ug/L
Diethyl phthalate 34336 Grab EPA 625/8270D 5 ND ug/L
Dimethyl phthalate 34341 Grab EPA 625/82700 5 ND ug/L
2,4-dinitrotoluene 34611 Grab EPA 625/8270D 5 ND ug/L
2,6-dinitrotoluene C0626 Grab EPA 625/8270D 5 ND ug/L
1,2-diphenylhydrazine - 34346 Grab EPA 625/82700 5 ND ug/L
Fluoranthene C0376 Grab EPA 625/8270D 5 ND ug/L
Fluorene 34381 Grab EPA 825/8270D 5 ND ug/L
Hexachlorobenzene C0700_ Grab EPA 625/8270D 5 ND_ ug/L
Hexachlorobutadiene 39702 Grab EPA 825/8270D 5 ND ug/L
Hexachlorocyclo-pentadiene 34386 Grab EPA 625/8270D 5 ND ug/L
Hexachloroethane 34396 Grab EPA 625/8270D 5 ND ug/L
Indeno(1,2,3-cd)pyrene _ 34403 Grab EPA625/8270D 5 ND ug/L
Isophorone 34408 Grab EPA 625/8270D 5 ND ug/L
Naphthalene 34696 Grab EPA 625/8270D 5 ND ug/L
Nitrobenzene 34447 Grab EPA 625/8270D 5 ND ug/L
N-nitrosodi-n-propylamine 34428 Grab EPA 625/8270D 5 ND ug/L
N-nitrosodimethylamine 34438 Grab EPA 625/8270D 5 ND ug/L
N-nitrosodiphenylamine 34433 Grab EPA 625/8270D 5 ND _ ug/L
11 Phenanthrene 34461 Grab EPA 625/8270D 5 ND ug/L
Pyrene 34469 Grab EPA 625/8270D 5 ND ug/L
1,2,4,-trichlorobenzene 34551 Grab EPA 624.8 260e 2 ND ug/L
"I certify,under penalty of law,that this document and all attachments were prepared under my direction or supervision in accordance
with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my
inquiry of the person or persons who managed the system,or those persons directly responsible for gathering the information,the
information submitted is,to the best of my knowledge and belief,true,accurate,and complete. I am aware that there are significant
penalties for submitting false information,including the possibility of fines and imprisonment for knowing violations."
Authorized Representative name
�� agar
Signature
/1a-3( il
Date
Form - DMR- PPA-1 Page 3

Annual Monitoring and Pollutant Scan
Facility Name Tryon Wastewater Plant ORC Deborah Bradley
Date of Sampling 6/7/2022 Phone [phone]
Analytical Laboratory ETS, INC.
Parameter Sample Analytical Quantitation Sample Units of
Parameter Code Type Method Level Result Measurement
Ammonia (as N) C0610 Composite SM 4500 NH3 D 0.1 0.21 mg/L
Chlorine (total residual, TRC) 50060 Grab 4500 Ci G 20 33 ug/L
Dissolved Oxygen 00300 Grab 4500-O-G 0.1 - 5.7 mg/L
„tttrite plus Nitrate Total(as N) 90630 __Composite jM45oo-NO3 H 9.2 .4 Q4 Hg/L
Total Kjeldahl Nitrogen 00625 Composite 351.2 0.1 1.30 mg/L
Oil and Grease 00556 Grab EPA 1664A 5 <5.0 mg/L
Total Phosphorus C0665 Composite 200.7 0.1 0.25 mg/L
Total Dissolved Solids 70295 Composite SM2540 C 10 340 mg/L
Hardness 00900 Composite SM2340 C 1 24 mg/L
Metals(total recoverable), cyanide and total phenols
Antimony 01097 Composite 200.8 0.005 <0.001 mg/L
Arsenic 01002 Composite 200.8 0.01 <0.001 mg/L
Beryllium 01012 Composite 200.8 0.001 <0.001 mg/L
Cadmium 01027 Composite 200.8 0.001 <0.001 mg/L
Chromium 01034 Composite 200.8 0.005 <0.001 mg/L
Copper 01042 Composite 200.8 0.001 0.009 mg/L
Lead 01051 Composite 200.8 0.005 <0.001 mg/L
Mercury(Method 1631E) COMER Composite 245.1 0.5 1.06 ng/L
Nickel 01067 Composite 200.8 0.01 <0.001 mg/L
Selenium 01147 Composite 200.8 0.01 <0.001_ mg/L
Silver 01077 Composite 200.8 0.005 <0.001 mg/L
Thallium 01059 Composite 200.8 0.001 <0.001 mg/L
Zinc 01092 Composite 200.8 0.01 0.016 mg/L
Cyanide 00720 Grab SM4500CN E 0.005 <0.005 mg/L
Total phenolic compounds 32730 Grab 420.1 0.005 <0.005 mg/L
Volatile organic compounds
Acrolein 34210 Grab EPA 624-82606 5
<5 ug/L
Acrylonitrile 34215 Grab EPA 624-8260B 5
Benzene 34030 Grab EPA 824-8260B 2
Bromoform 32104 Grab EPA 624-82606 2 <0.5 ug/L
Carbon Tetrachloride 32102 Grab EPA 624-8260B 2 <0.5 ug/L
Chlorobenzene 34301 Grab EPA 624-82608 2 <0.5 ug/L
Chlorodibromomethane 34306 Grab EPA 624-8260B 2 <0.5 ug/L
Chloroethane 85811 Grab EPA 624-8260B 2 <0.5 ug/L
2-chloroethyl vinyl ether 34576 Grab EPA 624-8260B 5 <0.5 ug/L
Chloroform 32106 Grab EPA 624-8260B 2 1.42 ug/L
Dichlorobromomethane 32101 Grab EPA 624-8260B 2 <0.5 ug/1,
1,1-dichloroethane 34496 Grab EPA 624-8260B 2 <0.5 ug/L
1,2-dichloroethane 32103 Grab EPA 624-8260B 2 <0.5 ug/L
Trans-1,2-dichloroethylene 34546 Grab EPA 624-8260B 2 <0.5 ug/L
1,1-dichloroethylene 34501 _ Grab EPA 624-8260B 2 <05 ug/L
Form - DMR- PPA-1 Page 1

Annual Monitoring and Pollutant Scan
Parameter Sample Analytical - Quantitation Sample Units of
Parameter Code Type Method Level Result Measurement
1,2-dichloropropane 34541 Grab EPA624-8260B 2 <0.5 ug/L
1,3-dichloropropylene 77163 Grab EPA 624-8260B 2 <0.5 ug/L
Ethylbenzene 34371 Grab EPA 624-8260B 2 <0.5 ug/L
Methyl Bromide 34413 Grab EPA 624-8260B 2 <0.5 ug/L
Methyl Chloride 34418 Grab EPA 624-8260B 2 <0.5 ug/L
Methylene Chloride 34423 Grab EPA 624-82608 2 <0.5 ug/L
1,1,2,2-tetrachloroethane 81549 Grab EPA 624-82608 2 <0.5 ug/L
Tetrachloroethylene 34475 Grab EPA 624-8260B 2 <0.5 ug/L
Toluene 34010 Grab EPA624-82608 2 <0.5 ug/L
1,1,1-trichloroethane 34506 Grab EPA624-8260B 2 <0.5 ug/L
1,1,2-trichloroethane 34511 Grab EPA624-8260B 2 <0.5 ug/L
Trichloroethylene 39180 Grab EPA 624-82606 2 <0.5 ug/L
Vinyl Chloride 39175 Grab EPA 624-82608 2 <0.5 ug/L
Acid-extractable compounds
P-chloro-m-creso 34452 Grab EPA 625/8270D 5 <0.5 ug/L
2-chlorophenol 34586 Grab EPA 625/8270D 5 <0.5 ug/L
2,4-dichlorophenol 34601 Grab EPA625/8270D 5 <0.5 ug/L
2,4-dimethylphenol 34606 Grab EPA 625/8270D 5 <0.5 ug/L
4,6-dinitro-o-cresol 34657 Grab EPA 625/8270D 5 <0.5 ug/L
2,4-dinitrophenol 34616 Grab EPA 625/8270D 10 <23.8 ug/L
2-nitrophenol 34591 Grab EPA 625/8270D 5 <23.8 ug/L
4-nitrophenol 34646 Grab EPA 625/8270D 5 <23.8 ug/L
Pentachlorophenol 39032 Grab EPA 625/8270D 10 <23.8 ug/L
Phenol 34694 Grab EPA625/8270D 5 <4.76 ug/L
2,4,6-trichlorophenol 34621 Grab EPA 625/8270D 5 <4.76 ug/L
Base-neutral compounds
Acenaphthene 34205 Grab EPA 625/8270D 5 <4.76 ug/L
Acenaphthylene 34200 Grab EPA 625/8270D 5 <4.76 ug/L
Anthracene - CO220 Grab EPA 625/8270D 5 <4.76 ug/L
Benzidine 39120 Grab EPA 625/8270D 80 <9.52 ug/L
Benzo(a)anthracene 34526 Grab EPA 625/8270D 5 <4.76 ug/L
Benzo(a)pyrene 34247 Grab EPA 625/8270D 5 <4.76 ug/L
3,4 benzofluoranthene 34230 Grab EPA625/8270D 5 <4.76 ug/L
Benzo(ghi)perylene 34521 Grab EPA 625/8270D 5 <4.76 ug/L
Benzo(k)fluoranthene 34242 Grab EPA 625/8270D 5 <4.76 ug/L
Bis (2-chloroethoxy) methane 34278 Grab EPA 625/8270D 5 <4.76 ug/L
Bis (2-chloroethyl) ether 34273 Grab EPA 625/8270D 5 <4.76 ug/L
Bis (2-chloroisopropyl)ether _ 34283 Grab EPA 625/8270D 5 <4.76 ug/L
Bis (2-ethylhexyl) phthalate 39100 Grab EPA 625/8270D 10 <4.76 ug/L
4-bromophenyl phenyl ether 34636 Grab EPA 625/8270D 5 <4.76 ug/L
Butyl benzyl phthalate 34292 Grab EPA 625/8270D 5 <4.76 ug/L
2-chloronaphthalene 34581 Grab EPA 625/8270D 5 _ <4.76 ug/L
4-chlorophenyl phenyl ether 34641 Grab EPA 625/8270D 5 <4.76 ug/L
Chrysene 34320 Grab EPA 625/8270D 5 <4.76 ug/L
Di-n-butyl phthalate 39110 Grab EPA 625/8270D 5 <4.76 ug/L
Form - DMR- PPA-1 Page 2

Annual Monitoring and Pollutant Scan
Parameter Sample Analytical Quantitation Sample Units of
Parameter Code Type Method Level Result Measurement
Di-n-octyl phthalate 34596 Grab EPA 625/8270D 5 <4.76 ug/L
Dibenzo(a,h)anthracene 34556 Grab EPA 625/8270D 5 <4.76 ug/L
1,2-dichlorobenzene 34536 Grab EPA 625/8270D 5 <0.5 ug/L
1,3-dichlorobenzene 34566 Grab EPA 625/82700 5 <0.5 ug/L
1,4-dichlorobenzene 34571 _ Grab EPA625/8270D 5 <0.5 ug/L
3,3-dichlorobenzidine 34631 Grab EPA625/8270D 5 <9.52 ug/L
Diethyl phthalate 34336 Grab EPA 625/8270D 5 <4.76 ug/L
Dimethyl phthalate 34341 Grab EPA 625/8270D 5 <4.76 ug/L
2,4-dinitrotoluene 34611 Grab EPA 625/8270D 5 <4.76 ug/L
2,6-dinitrotoluene C0626 Grab EPA 625/8270D 5 <4.76 ug/L
r
1,2-diphenylhydrazine 34346 Grab EPA 625/8270D 5 <4.76 ug/L
Fluoranthene _ C0376 Grab , EPA625/8270D 5 <4.76 ug/L
Fluorene 34381 Grab EPA 625/8270D 5 <4.76 ug/L
Hexachlorobenzene C0700 Grab EPA625/8270D 5 <4.76 ug/L
Hexachlorobutadiene 39702 Grab EPA 625/8270D 5 <4.76 ug/L
Hexachlorocyclo-pentadiene 34386 Grab EPA 625/8270D 5 <23.8 ug/L
Hexachloroethane 34396 Grab EPA 625/8270D 5 <4.76 ug/L
Indeno(1,2,3-cd)pyrene 34403 Grab EPA625/8270D 5 <4.76 ug/L
Isophorone 34408 Grab EPA625/82700 5 <4.76 ug/L
`
Naphthalene 34696 Grab EPA 625/8270D 5 <4.76 ug/L
Nitrobenzene 34447 Grab EPA 625/8270D 5 <4.76 ug/L
N-nitrosodi-n-propylamine 34428 Grab EPA 625/8270D 5 <4.76 ug/L
N-nitrosodimethylamine 34438 Grab EPA 625/8270D 5 <4.76 ug/L
N-nitrosodiphenylamine 34433 Grab EPA 625/8270D 5 <4.76 ug/L
Phenanthrene 34461 Grab EPA 625/8270D 5 <4.76 ug/L
Pyrene 34469 Grab EPA 625/8270D 5 <4.76 ug/L
1,2,4,-trichlorobenzene 34551 Grab EPA 624-8260B 2 <0.5 ug/L
"I certify,under penalty of law,that this document and all attachments were prepared under my direction or supervision in accordance
with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my
inquiry of the person or persons who managed the system,or those persons directly responsible for gathering the information,the
information submitted is,to the best of my knowledge and belief,true,accurate,and complete. I am aware that there are significant
penalties for submitting false information, including the possibility of fines and imprisonment for knowing violations."
Authorized Representative name
,------9--/-24--F-L<J,-- ZA4._Cd..14
Signature U
7//4-/32-
Date
Form - DMR- PPA-1 Page 3

Annual Monitoring and Pollutant Scan
Facility Name Tryon Wastewater Plant ORC Deborah Bradley
Date of Sampling 12/12/2023 Phone [phone]
Analytical Laboratory ETS,INC.
Parameter Sample Analytical Quantitation Sample Units of
Parameter Code Type Method Level Result Measurement
Ammonia(as N) C0610 Composite SM 4500 NH3 D 0.1 0.54 mg/L
Chlorine(total residual,TRC) 50060 Grab 4500 Ci G 20 34 ug/L
Dissolved Oxygen 00300 Grab 4500-O-G 0.1 6.8 mg/L
Nitrite plus Nitrate Total(as N) 00630 Composite SM4500-NO3 H 0.2 2.77 mg/L
Total Kjeldahl Nitrogen 00625 Composite 351.2 0.1 2.80 mg/L
Oil and Grease 00556 Grab EPA 1664A 5 <5.15 mg/L
Total Phosphorus C0665 Composite 200.7 0.1 0.54 mg/L
Total Dissolved Solids 70295 Composite SM2540 C 10 240 mg/L__
Hardness 00900 Composite SM2340 C 1 39 mg/L
Metals(total recoverable), cyanide and total phenols
Antimony 01097 Composite 200.8 0.005 <0.001 mg/L
Arsenic 01002 Composite 200.8 0.01 <0.001 mg/L
Beryllium 01012 Composite 200.8 0.001 <0.001 mg/L
Cadmium 01027 Composite 200.8 0.001 <0.001 mg/L
Chromium 01034 Composite 200.8 0.005 <0.001 mg/L
Copper 01042 Composite 200.8 0.001 0.0179 mg/L
Lead 01051 Composite 200.8 0.005 0.0011 mg/L
Mercury(Method 1631E) COMER Composite 245.1 0.5 3.32 ng/L
Nickel 01067 Composite 200.8 0.01 <0.001 mg/L
Selenium 01147 Composite 200.8 0.01 <0.002 mg/L
Silver 01077 Composite 200.8 0.005 <0.001 mg/L
Thallium 01059 Composite 200.8 0.001 <0.001 mg/L
Zinc 01092 Composite 200.8 0.01 0.0208 mg/L
Cyanide 00720 Grab SM4500CN E 0.005 0.005 mg/L
Total phenolic compounds 32730 Grab 420.1 0.005 <0.005 mg/L
Volatile organic compounds
Acrolein 34210 , Grab EPA 624-8260B 5 <5 ' ug/L
Acrylonitrile 34215 Grab EPA 624-8260B 5 <5 ug/L
Benzene 34030 Grab EPA624-8260B 2 <0.5 ug/L
<0.5 ug/L
32104 Grab EPA 62482606 2 g/
Bromoform -.-
Carbon Tetrachloride 32102 Grab EPA 624-82606 2 _ <0.5 ug/L
Chlorobenzene 34301 Grab EPA 624-8260B 2 <0.5 ug/L
Chlorodibromomethane 34306 Grab` EPA 624-8260B 2 <0.5 ug/L
Chloroethane 85811 Grab EPA 624-8260B 2 <0.5 ug/L
2-chloroethyl vinyl ether 34576 Grab EPA 624-8260B 5 <0.5 ug/L
Chloroform 32106 Grab EPA624-8260B 2 <0.5 ug/L
Dichlorobromomethane 32101 Grab EPA624-8260B 2 <0.5 ug/L
1,1-dichloroethane 34496 Grab EPA624-8260B 2 <0.5 ug/L
1,2-dichloroethane 32103 Grab EPA 6248260E 2 <0.5 ug/L
Trans-1,2-dichloroethylene 34546 Grab EPA624-8260B 2 t <0.5 ug/L
1,1-dichloroethylene 34501 Grab EPA 624-82608 2 <05 _ ug/L
Form - DMR- PPA-1 Page 1

Annual Monitoring and Pollutant Scan
Parameter Sample Analytical Quantitation Sample Units of
Parameter Code Type Method Level Result Measurement
1,2-dichloropropane 34541 Grab EPA 624-8260B 2 <0.5 ug/L
1,3-dichloropropylene 77163 Grab EPA 624-8260B 2 <0.5 ug/L
Ethylbenzene 34371 Grab EPA 624-8260B 2 <0.5 ug/L
Methyl Bromide 34413 Grab EPA 624-8260B 2 <0.5 ug/L
Methyl Chloride 34418 Grab EPA 6248260E 2 <0.5 ug/L
Methylene Chloride 34423 Grab EPA 624-8260B 2 <0.5 ug/L
1,1,2,2-tetrachloroethane 81549 Grab EPA624-8260B 2 <0.5 ug/L
Tetrachloroethylene 34475 Grab EPA 624-8260B 2 <0.5 ug/L
Toluene 34010 Grab EPA 624-8260B 2 <0.5 ug/L
1,1,1-trichloroethane 34506 Grab EPA624-8260B 2 <0.5 ug/L
1,1,2-trichioroethane 34511 Grab EPA 824-8260B 2 <0.5 ug/I,
Trichloroethylene 39180 Grab EPA624-8260B 2 <0.5 ug/L
Vinyl Chloride 39175 Grab EPA 624-8260B 2 <0.5 ug/L
Acid-extractable compounds
P-chloro-m-creso 34452 Grab EPA 625/8270D 5 <0.5 ug/L
2-chlorophenol 34586 Grab EPA 625/8270D 5 <0.5 ug/L
2,4-dichlorophenol 34601 Grab EPA 625/8270D 5 <0.5 ug/L
2,4-dimethylphenol 34606 Grab EPA 625/8270D 5 <0.5 ug/L
4,6-dinitro-o-cresol 34657 Grab EPA 625/8270D 5 <0.5 ug/L
'2,4-dinitrophenol 34616 Grab EPA 625/8270D 10 <23.8 ug/L
2-nitrophenol 34591 Grab EPA 625/8270D 5 <23.8 ug/L
4-nitrophenol 34646 Grab EPA 625/8270D 5 <23.8 ug/L
Pentachlorophenol 39032 Grab EPA 625/82700 10 <23.8 ug/L
Phenol 34694 Grab EPA 625/8270D 5 <4.76 ug/L
2,4,6-trichlorophenol 34621 Grab EPA 625/8270D 5 <4.76 ug/L
Base-neutral compounds
Acenaphthene 34205 Grab EPA 625/8270D 5 <4.76 ug/L
Acenaphthylene 34200 Grab EPA 625/8270D 5 <4.76 ug/L
Anthracene CO220 Grab EPA 625/8270D 5 <4.76 ug/L
Benzidine 39120 Grab EPA 625/8270D 80 <9.52 ug/L
Benzo(a)anthracene 34526 Grab EPA 625/8270D 5 <4.76 ug/L
Benzo(a)pyrene 34247 Grab EPA 625/8270D 5 <4.76 ug/L
3,4 benzofluoranthene 34230 Grab EPA625/8270D 5 <4.76 ug/L
Benzo(ghi)perylene 34521 Grab EPA 625/82700 5 <4.76 ug/L
Benzo(k)fluoranthene 34242 Grab EPA 625/8270D 5 <4.76 ug/L
Bis(2-chloroethoxy) methane 34278 Grab EPA 625/8270D 5 <4.76 ug/L
Bis(2-chloroethyl) ether 34273 Grab EPA 625/8270D 5 <4.76 ug/L
Bis(2-chloroisopropyl) ether 34283 Grab EPA 625/8270D 5 <4.76 ug/L
Bis(2-ethylhexyl) phthalate 39100 Grab EPA 625/8270D 10 <4.76 ug/L
4-bromophenyl phenyl ether 34636 Grab EPA 625/8270D 5 <4.76 ug/L
Butyl benzyl phthalate 34292 Grab EPA 625/8270D 5 <4.76 ug/L
2-chloronaphthalene 34581 Grab EPA 625/8270D 5 <4.76 ug/L
4-chlorophenyl phenyl ether 34641 Grab EPA 625/82700 5 <4.76 ug/L
Chrysene 34320 Grab EPA 625/8270D 5 <4.76 ug/L
Di-n-butyl phthalate 39110 Grab EPA 625/8270D 5 <4.76 ug/L
Form - DMR- PPA-1 Page 2

Annual Monitoring and Pollutant Scan
Parameter Sample Analytical Quantitation Sample Units of
Parameter Code. Type Method Level Result Measurement
Di-n-octyl phthalate 34596 Grab EPA 625/8270D 5 <4.76 ug/L
Dibenzo(a,h)anthracene 34556 Grab EPA 625/8270D 5 <4.76 ug/L
1,2-dichlorobenzene 34536 Grab EPA625/8270D 5 <0.5 ug/L
1,3-dichlorobenzene 34566 Grab EPA625/8270D 5 <0.5 ug/L
1,4-dichlorobenzene 34571 Grab EPA 625/8270D 5 <0.5 ug/L
3,3-dichlorobenzidine 34631 Grab EPA 625/8270D 5 10.2 ug/L
Diethyl phthalate 34336 Grab EPA 625/82700 5 <4.76 ug/L
Dimethyl phthalate 34341 Grab EPA 625/8270D 5 <4.76 ug/L
2,4-dinitrotoluene 34611 Grab EPA 625/8270D 5 <4.76 ug/L
2,6-dinitrotoluene C0626 Grab EPA 625/8270D 5 <4.76 ug/L
1,2-diphenylhydrazine 34346 Grab EPA 625/8270D 5 <4.76 ug/L
Fluoranthene C0376 Grab EPA 625/82700 5 <4.76 ug/L
Fluorene 34381 Grab EPA 625/8270D 5 <4.76 ug/L
Hexachlorobenzene C0700 Grab EPA 625/8270D 5 <4.76 ug/L
Hexachlorobutadiene 39702 Grab EPA 625/8270D 5 <4.76 ug/L
Hexachlorocyclo-pentadiene 34386 Grab EPA 625/8270D 5 <23.8 ug/L
Hexachloroethane 34396 Grab EPA 625/8270D 5 <4.76 ug/L
Indeno(1,2,3-cd)pyrene 34403 Grab EPA625/8270D 5 <4.76 ug/L
Isophorone 34408 Grab EPA 625/8270D 5 <4.76 ug/L
Naphthalene 34696 Grab EPA625/8270D 5 <4.76 ug/L
Nitrobenzene 34447 Grab EPA 625/82700 5 <4.76 ug/L
N-nitrosodi-n-propylamine 34428 Grab EPA 625/8270D 5 <4.76 ug/L
N-nitrosodimethylamine 34438 Grab EPA625/8270D 5 <4.76 ug/L
N-nitrosodiphenylamine 34433 Grab EPA 625/8270D 5 <4.76 ug/L
Phenanthrene 34461 Grab EPA625/8270D 5 <4.76 _ ug/L
Pyrene 34469 Grab EPA 625/8270D 5 <4.76 ug/L
1,2,4,-tichlorobenzene 34551 Grab EPA 624-82608 2 <0.5 ug/L
"I certify, under penalty of law,that this document and all attachments were prepared under my direction or supervision in accordance
with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my
inquiry of the person or persons who managed the system,or those persons directly responsible for gathering the information,the
information submitted is,to the best of my knowledge and belief,true,accurate,and complete. I am aware that there are significant
penalties for submitting false information,including the possibility of fines and imprisonment for knowing violations."
Authorized Representative name
Signature
Date
Form - DMR- PPA-1 Page 3

PO Box 7565
Asheville,NC 28802
In.�m.n„re,nn9sa���x nt Phone: [phone]
Fax: [phone]
March 23,2023
Ms. Deborah Bradley
Town of Tryon
423 North Trade Street
Tryon,NC 28782
ETS Project Number: 17753
Test Start Date: March 15,2023
Facility Tested: Tryon WWTP
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Parameter Result
Code Test Procedure,Method Number
PASS FAIL
TGP3B North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure V
EPA-821-R-02-013,Method 1002.0 A
All toxicity testing results required as part of your permit must be entered on the Effluent Discharge
Monitoring Form(MR-1)for the month in which it was performed,using the parameter code TGP3B.
Additionally,the DMR Reporting Form AT-1 must be signed and e-mailed or mailed to the following
address:
[e-mail]
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh,NC 27699-1621
Please call if you have any questions concerning these results.
Sincerely,
Jim Sumner
Laboratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
2 of 10 3/27/2023,8:24 AP

PO Box 7565
Asheville,NC 28802
Phone: [phone]
EmbonmenJ4l Tenmq Solution.Inc Fax: [phone]
Effluent Toxicity Report Form-Chronic Pass/Fail and Acute LCso Date: March 23,2023
Facility: Town of Tryon NPDES#: NC 0021601 Pipe#: 001 County: Polk
Tryon WWTP
Laboratory Performing Test: Environmental Testing Solutions,Inc.,Certificate#037 Comments.
Signature of Operator in Responsible Charge(ORC):C- ,/
ORC Phone/E-mail: deil Arad/ [iJ ' ,'/ 424,2 Protect It: 17753
Signature of Laboratory Supervisor: Sample a: 230314.11,230316 OS
e-Mail to: ATForms.AT8 atncdenr.gov
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-Stat/Rank Sum -2.624
Raleigh,NC 27699-1621 1-Tailed Critical 2.508
%Reduction: -7.8
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent Average
Mortality Reproduction
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 control Control
Number of Young Produced 32 33 37 30 30 33 32 31 30 30 35 32 0.0 32.1
Adult Survival: (L)ive,(D)ead LLLL L L L L L L L L Treatment 2 Treatment 2
0.0 34.6
Effluent Percentage 37% Control CV
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 6.8 PASS FAIL
Number of Young Produced 37 34 33 33 35 31 33 39 38 32 34 36 "°I°'r'""""0`""`"`
Jrd bne4
Adult Survival: (L)ive,(D)ead L L ILL IL L L L L L 100.0
pH(S.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date: March 15,2023
Control 8.05 8.03 7.96 8.08 7.98 8.05
Treatment 2 7.96 8.02 7.84 8.04 7.82 8.01 Collection(Start)Date:
r, 0 t - „ Sample 1 03-13-23 Sample 2 03-15-23
C A C C
'-' v, N
D.O.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control 7.7 7.9 7.8 7.8 7.9 8.0 Grab Comp. Duration
Treatment 2 8.1 8.0 7.9 8.0 8.0 8.1 Sample 1 • X 24-h c
o i' a d
Sample 2 . X 24.h 5 5 E E
LCso/Acute Toxicity Test Alkalinity(mg CaCo5/L) 60
(Mortality expressed as%,combining replicates.) Hardness(mg CaCOJ/L) 84
Conductivity(umhos/cm) 313,292 299 275 267
Concentration(%) Total Residual Chlorine(mg/0 <0.10 <0.10
Mortality(%) Sample Temp.at Receipt(°C) 1.7 2.1
LCSO= Method of Determination
95%Confidence Limits Trimmed Spearman Karber r
wta
to Probit
Other: Control
High Conc.
Organism Tested: Duration: pH(S.U.) DO(mg/L)
DWR Report Form AT-1
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Page loll
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Cerlodaphnia dubia
(EPA-821-R-02-013 Method 1002.0,NC Modification-December 2010,Version 3.0)-Control Bench Sheet
Control p: '' Date: 03-15-23
rest Grouping Information: Test Organism information: --
Facility Project R Organism Source: In-house Culture
6 W MSv 1\le I T14 S Age <24-hours old
0`�-
S Zti�`IO 1-t15.1 Source(cultureboard): o •t3 A ob ' e
4 NC,b wrL 1VI5, Replicate a 1 la 134:01 S.i 611 7 i 8�931 H e l l
3 N of0+� r1 tq Culture board cup a.1151
MJgi
2 HAittt21P 4. 0n.. t-nci Date and time organisms were born d-,-V-t oioo - 1010
1-Control between:
Average transfer volume: <0.25 mi.
Transfer bowl Information: pH(S.U.): TS,0 0
Daily Renewal Information: Temmoatorerdl: b .0
feat lniOal Ion,renewal,'reeding nu
Oay Date temdnalbn MOW ages, selenasWm TW7 Leaarlpn pandemleing
Time Analyse Balch Balch Incubator/Shell Tempiaee
0 03.15-23 Iwu 'vr
i 1 :(1%.X.Sb cy.ot t� ts3•o1u i--Gk 81ACK
I 03-16-23 ° �,'j/,}tat5
2 03-17-23 a Ivre,a.r�33oA•L!$
3 03.18.23 r 845 q{f
4 03-19-23 7,1s 4d Air-
5
03-20-23 am 0a;r{`.1-0. p
"1` D
6 03.21.23 I`'> /' ,7
7 03-22.23 tawl�'t` v/t'A`
•Oreanlemf led dale 50 al NC Sdeeot Otml and 50 tar'Mailer renu:ale third NaMyslep retreat obmar SN 27E5935..
Chemical Analyses: Initiation Renewal One
Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst I3L V '' el— k -8L (A.. 13(. f3L. AL 1A.
pH(S.U.) } 7,.9( 8.or 7.AS Sr.0 5
Dissolved oxygen Ova)
Conductivity(pmhos/cm) �.$ 9 0•'�
Control,MHSW ;l3 2'�2 29q
•Alkalinity(me/LCaCO') U0
'Hardness tmg/L CaC0,1 p
_ O y gg,Li.t111
'Temperature(%C) 1A.1 .,4 °► Z'I.to -Ls. 1.. ii4 1 VA
'Anahp idea:died for call day.perfumed pt.dioaoh d m trn ad eumdueuein mespmneeu ally Temperatures p-rfarmed al On too of lira eriliauve.rmrnal or lernmxian by tic
analyst idvo:i d in the Daily Raw,al Inf alpn table AlLatinity and hardness performed be sir analyst,itdeniitcd nnIiv tort kind,shorn aM vmuenkd so isi,bons,site:
earame ter Reporting limit Method number Meter Serial number
pN 015.U. SM 1500-H,8-1011 Acawnel A820 93312452
Dissolvednaygen 1.0 NIA 5M4500.O G.2016 151Madel52Ct n0104324
Co,MuOMty 14.9 patinas/an SM 25101-201 I sentinel 0R10 91312452
A8a0nity 5.Omg CaCO,/L 5M2320 8-2013 accumet A420 93112..52
Nerdnees 5.0 ing CaCDSI SM 2310 C-1011 Not applicable riot appliubie
Tool nddadr o,tw 0.1mg/L O1ON97-70-1977 AO<atrnet A8150 923a9123
Temperature n.1•C SM20009-3150 DlptalThermometer 1304/ARS
Survival and Reproduction Data:
Day Observations Replicate number
1 2 3 4 5 6 7 8 9 10 Il 12
2 Adult mortality `
Ran awal One Icetme,Fir l— ,-' `--- `- ` L. ` ` \-.- L. `,_,
Number of broods present L Ly ct- L L L L L Li. L L L.
5 Number of young produced Igo 1`� (6 1� Ito 1� Li LS t� IS t� l3
Renewal Two Adult mortality
IL a tire,0a dead) `- �-- L- L. L. `, t`
Number of broods present L �n1L t tI�a 1 1 ` ', I I t '` t I'
Number of young produced it. t-L 1�{ 1 Lsp- 1 2 0 l 4 1 b 1-� 1 cL� 1 p 1 IQ_
7 Total young produced 5Z ,� 31 J O
Final 3u 30 7J3 31 51 30 30 3S '3'L
Final adult mortality 11
•
•
•
•
x for 3rd Broods x x X r yt- )(,- X ) x x � >L -
Control Acceptance Criteria:
/"� /-
%olMale Adults(s 20%) O . Mean Offspring/Female
sz.l
%Adults having 3rd Broods(2 80Y8) (�1- R.15 pfrspnng/sufvivtne female)
%Mortality(5 20%) 0 l_ X N(<40%) 45 8 7
4 of 10
3/27/2021 R•24 A ti

Page 1 of 1
North Carolina Chronic Pass/Fait Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0,NC Modification-December 2010,Version 3.0)-Test Bench Sheet
Paired with Control# Z Date: (1S-‘5•L'5
Client Tryon WWTP NPDEEs h NC 0021601
Facility . Outfall 001
Project 9 11-1S'S County Polk
Test Concentration(Chronic Limit) 37% Dilution tel m! Total volume
oreparation: Sample Dilution water nil
Samples were not aerated or treated unless otherwise noted on this form.Control,dilution water ._ 111 189 300
and test renewal information are ncluded on the Control Bench Sheer indicated above.
Chemical Analyses: Initiation Renewal One Renewal Two
Initial" Final Initial + Final Initial Final
Concentration Analyst 13L y A- et_ vk 13Z LaU g it 13L pZ t
pH l5.U.) 7.q6. '� 7,7 X. OfJ- 7.8'.2 g.D/�
Test Dissolved oxygen(mg/I.) OU•I O D '2 X. 8S.t S•C e-1
Concentration Conductivity(pmhos/cm) 24 if li' 2 y3ff 2 q 3
•Temperature CC) Zit q �i
pH(S.U.) 7.' 3 7.99
Dissolved oxygen(mg/l) 61/ 0.O
100% Conductivity(µmhos/cm) 275 .2 G 7
•Total residual chlorine(my!) L,0,10 <0•10
Sample number Sample1 1rjosM•1\ Sample2 -.1aOS1b US
'Analyst identified for cad,day,performed pld dissolved oxygen and conductivity measurements only. Temperature,performed at the lime of test initiation,renewal or
termination by the analyst identified in the Daily Rene•wt Information table located en the Control Bench Sheet.Total residual chlorine performed by the analyst identified on
the Total Residual Chlorine Bench Sheet and transcribed io this berth sheet
Survival and Reproduction Data (performed of test concentration):
Day Observations Replicate number
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality
Renewal One iL_Live,0=dead) A.._ \— S ``-- t— N-- L \— �---
Number of broods present i L L - It L .l _L L, L L �i. 1-t.5 Number of young produced 1& l rj L3 it. t,la, I l�j. ►& 1 L 1 t LL,
t [Ti.!`
Renewal Two Adult mortality 6
(L=Live,O=dead) L. • L- `_ ` ` `-
Number of broods present L1 LL L L LL LL(�_ L LL I L LL IL
Number of young produced (l 14 I (I t't I6 1.1 ZO Ig--. Q !g
7 Total young produced 1
Final 3-13A 3} 35 3\ N3 35 3& 31- 34 Mc
Final adult mortality
It=1rve,0=dead) 1___ l_ k_ l' l-" L.- t 1-
Test was Initiated using Sample 1.Sample 2 was used for Renewals One(day 2)and Two(day 5).Samples were diluted to the test concentration prior so use with moderately
hard synthetic water and warmed to 25.0±1.0•C in a warm water bath.
Comments:
Test Results and Statistical Analyses:
Test results Statistics
%Mortality O T t-Stat or
Rank Sum -2./.7.`I
Mean offspring 1-Tailed r�
per female 3� �' Critical �.so
56 Reduction ���(
1. 8 PASS or FAIL flASS from control
SOP AT12-Reyislon a-exhibit ATI?2
5 of 10 3/27/2023, 8:24 AM
1

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0
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0
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fn.bonmmbl tnUne f oretlenL Inc.
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1002.0)
Species: Ceriodaphnia dubia
Facility: Tryon WWTP r^r„an,
ry ,.v.
Test start date: March 15,2023 ,M,w
Project number: 17753 �n!n °^° ^
Sample numbers: 230314.11,230316,05
Concentration Oa Number of young produced by replicate number Survival Average Coefficient of Percent reduction from
(%) y 1 2 3 4 5 6 7 g 1%I reproduction variation 1%) control 1%)
_ 9 10 11 12 (offspring/female)
5 16 _ 14 18 14 16 13 18 15 13 15 17 13
Control 7 16 19 19 16 14 20 14 16 17 15 18 19 100 32.1 6.8 Not applicable
Total 32 33 37 30 30 33 32 31 30 30 35 32
5 18 15 13 16 16 14 15 18 18 14 15 17
37% 7 19 19 20 17 19 17 18 21 20 18 19 19 100 34.6 7.1
-7.8
Total 37 34 33 33 35 31 33 39 38 32 34 36
Dunnett's MSD value: 2.390 MSD= Minimum Significant Difference
PMSD: 7.4 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10ih percentile) =13%.
Upper PMSD bound determined by USEPA(90'"percentile)=47%.
Lower and upper PMSD bounds were determined from the 10.'and 90th percentile,respectively,of PMSD data from EPA's WET Interlaboratory
Variability Study(USEPA,2001a;USEPA,20016). The lower PMSD bound represents a practical limit to the sensitivity of the test method and is
not a minimum acceptance criteria.
USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-B.01-004 and EPA-821.8-01.005,US
Environmental Protection Agency,Cincinnati,0H.
t.�
N
O
tv
Ca
W
00 C
is:,
.p ,C.,
a

Entered by,
Kelley Keenan
Statistical Analyses maser
wn,ne�
Emirenmenral Tetunq Solutions.Inc
Ceriodaphnia Survival and Reproduction Test-Reproduction
Start Date: 3/15/2023 Test ID: CdPFCRNC Sample ID: Tryon
End Date: 3/22/2023 Lab ID: ETS-Envir.Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol. FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
Comments:
Conc-% 1 2 3 4 5 6 7 8 9 10
D-Control 32.000 33.000 37.000 30.000 30.000 33.000 32.000 31 000 30.000 30.000
D-Control 35.000 32 000
37 37.000 34.000 33.000 33.000 35.000 31.000 33.000 39.000 38.000 32.000
37 34.000 36.000
Transform:Untransforrned 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
0-Control 32.083 1.0000 32.083 30.000 37.000 6.836 12
37 34.583 1.0779 34.583 31.000 39.000 7.132 12 -2.624 2.508 2.390
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution(p>0.01) 0.93689 0.884 0.69906 -0.2768
F-Test indicates equal variances(p=0.70) 1.26457 5.31963
Hypothesis Test(1-tail,0.01) MSDu MSDp MSB MSE F-Prob df
Homoscedastic t Test indicates no significant differences 2.38993 0.07449 37.5 5 44697 0.0155 1.22
Treatments vs D-Control
Dose-Response Plot
45
40
35 0
0--
30+ -1-tail.0.01 level
.2 of significance
u 25
c 20
a
u
c 15
10
5
0
a
a
U
0
7 of 10 3/27/2023,8:24 AM

351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Tryon WWTP NPDES#: NC0021601 Pipe#: 001 County: Polk
Purchase order:
Species: Ceriadap/inia dubia Effluent dilution: 37%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Composite sample: Sample location: ,tiertIa�, ��/, /Me r�4
Start date: J-/3- 13 Time: 0 7:06 Volume collected f6rgesting: 4t)�L O
End date: .-t - )3 Time: D VG> Number of containers filled for Costing:
Number of samples per hour: `f Method of transport to laboratory: k4C,
Chilled during collection? L-47 Comments:
_3�'
If chilled,specify temperature:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample collected by:
3-ill-a3
�g rr1/ih.tI L. Y�ilehCu� f 4> 6d
t m. Me animas
Relinquished by: Received by:
LQ 3 r`I'- 3-/t/-23
-tt 414'' (YR-7-) Veuro c-W h r AY/.`��-(*.I AF,
Ihml Sq..= 7 Ibm.nd tone Ihis ) /Sapanuto Daft and unx
Relinquished by: Received by:
Pt/-41-3
ke si cl) + i, r 9tcf�7`, )0 10 0 C1 iret t,.t l Jj
komatale
Sample receipt information: (to be completed by ETS personnel)
Reanquished to ETS by: Received at ETS by:
31i1(9� t‘l 4S
IM s�e.m.. nam aid m� nw s,twtoe
Custody seals intact?: n1=1. Sample temperature upon receipt at ETS(°C): 1,,.1 .
Y. No la mini
Samples received in good condition?: =
Ye. No Total residual chlorine upon receipt at ETS: 1
(DPD PresencelAbbsense Indicator.MDL-0.10 mg/LI ~°""
Tracking number: iJ P, /
Project number:1_2V Sample number: , b ly I I
Comments:
8 of 10 3/27/2023,8:24 AM

351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Tryon WWTP NPDESt): NC0021601 Pipe it: 001 County: Polk
Purchase order:
Species: Ceriodapinia(labiaEffluent dilution: 37%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector) __
t,
i
Composite sample: Sample location:
Stars date: 3/iS/, Time: 6 * '' p.rXet
d Volume collected for testing: 4„t4Aj}el
End date: 3//./a-3 Time: p Sk,0 Number of containers filled for testing: /
:! Number of samples per hour: 4 Method of transport to laboratory:
Chilled during collection? ��
r�2n Comments:
li If chilled,specify temperature: 3'
l Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. 1
n
l '!
Sample custody: (to be completed by sample collector and facility personnel)
li
4 BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE ..
Sample collected by: r,
L �� 13/ }3 I
1x+�[un r I �rCry/,M,el tSao
5 1e` Iloc aJ time
Relinquished by:
!Received by:
v ��A 3 6 J' -� 3 JG 3
Lk.,...„-,-,„,.. ,,i,a an ,-..)9.„,/ 1 fib/ 'mil /.s
rlGrrsi JGd13r� )0���1�rY��QQdJ/// l dy
Pant s,p.ure Owe and lune
�� c
�mM 3yaaavc lblc angle Relinquished by:
Received by:
� �MTm Scenalxc rim and umc
Ron aatcand
Ei
Sample receipt information: pleted by ETS personnel) r ,,�
(to be com
Relinquished to ETS by: Received at ETS by:
I3)ldjl3 I I
ke,rceivrA beiR f .t- 1D% J I vKwek l k — 1 f 'io 0
Rva Gpuwre Oil,and rms.
Print ltrr°t, Dec aN tmc
Custody seals Intact?: r---i ED Sample temperature upon receipt at ETS(°C):
Samples received in good condition?: r-q-n
ra, Na
Total residual chlorine upon receipt at ETS:
N (DPD Pracncc7Absense lndiealor•MDL-e.10 mall) r`".' Al...
Tracking number: A I vj�
Project number:l 7 Sample number: /V 4.316.0'
Comments:
9 of 10 3/27/2023,8:24 AM
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� ' �� Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
Environmental Testing Solutions,Inc Source: In-house Culture
1.14 ! till I 1 I I I I I I I 1 I I I I
1.12 - Control Limits (±2 Standard Deviations) -
1.10 - •
•
1.08 - • • • •
1.06 -
1.04 -
Z
1.02 -
v 1 1 1 1 1 1 1 1 1 1 1 1 1 I 1 I 1 1 1 1
N - I I I 'I •-I I I I I I 1 1 I 1 I 1 I 'i -T -I
U _
> 1.15 - _
a Warning Limits
1.05 -
1.00 -
0.95 I I I I ( I I I I I 1 I 1 1 I 1 I I 1 1
10.05 11A1 1ti°1 o'L°4 01,0(010$o3-p oaAS c o3 001 0.0.1 DaA2 t 1;1a°p��A$�0 A$11,06 0.0'6.01 pee��
Test date
• 7-day IC25=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodophnia reproduction(calculated using ToxCalc).
Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
• -- -•-- Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
••.-•• Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
USEPA Warning Limits (mean logarithmic IC15±Ski,converted to anti-logarithmic values,
Salo=10t''percentile of CVs reported nationally by USEPA)
()
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PO Box 7565
Asheville,NC 28802
Phone: [phone]
En„,,,,,n„ta„„u„y So,°lom.M. Fax: [phone]
Effluent Toxicity Report Form-Chronic Pass/Fail and Acute LCso Date: June 22,2023
Facility: Town of Tryon NPDES U: NC 0021601 Pipe 44: 001 County: Polk
Tryon WWTP
Laboratory Performing Test: Environmental Testing solutions,Inc.,Certificate#037 Comments
Signature of Operator in Responsible Charge(ORC.�//�JJ _ e` __
ORC Phone/E-mail: 04.J3�,t,Lri,, ) Li�Y11i4t49 r aitn � Project a: 17991
Signature of Laboratory Supervisor: Sample Y- 230613.11,230615.02
e-Mail to: [e-mail]
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-Stat/Rank Sum -9.303
Raleigh,NC 27699-1621 1-Tailed Critical 2.508
%Reduction: -21.8
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent Avenge
Mortality Reproduction
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Control Control
Number of Young Produced 27 28 28 28 26 30 31 28 30 31 28 29 0.0 28.7
Adult Survival: (L)ive,(D)ead IL L I L L L LLL L L Treatment 2 Treatment 2
0.0 34.9
Effluent Percentage 37% Control CV
Treatment 2 Or
ganisms anisms 1 2 3 4 5 6 7 8 9 10 11 12
5.4 PASS FAIL
%34 32 37 35 37 33 37 33 35 34 36 36 pnvp1ejirvtntpedun°3
Number of Young Produced IA bowl X °
Adult Survival: (L)ive,(0)ead L L L L L L L L L L L L 100.0
pH(S.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date: June 14,2023
Control 7.78 7.85 7.70 7.92 7.75 7.90
Treatment 2 7.88 7.95 7.84 7.95 7.91 7.95 Collection(Start)Date:
c q 0 5 � Sample 1 06-12-23 Sample 2 06-14-23
W E.
D.O.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control 7.8 7.6 7.7 7.9 7.9 7.6 Grab Comp.Duration
Treatment 2 8-0 8.0 7.9 8.0 7.9 7.7 Sample 1 • X 3e.h c u a
Sample 2 X to-h ? 3 A m
O N V
LCso/Acute Toxicity Test Alkalinity(mg CaCO3/1) 61
(Mortality expressed as%,combining replicates.) Hardness(mgCaCO3/t) 86 .
Conductivity(µmhos/cm) 301,293,303 286 273
Concentration(%) Total Residual Chlorine(mg/1.) <0.10 <0.10
Mortality(%) Sample Temp.at Receipt(°C) 1.5 3.6
LC50= Method of Determination
95%Confidence Limits ^Trimmed Spearman Karber , W A
to Probit N
-Other: Control
High Conc
Organism Tested: Duration: pH(S.U.) DO(mg/1.)
DWR Report Form AT-1
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North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodophnia dubia
(EPA-821-R-02-013 Method 100Z.0,NC Modification-December 2010,Version 3.0)-Control Bench Sheet
Control @- Date: 06-13-23
Test Grouping Information: Test Organism Information:
Facility Proiect if i Organism Source: In-house Culture
6 1 r Ot\ L7 t Age: <24-hours old
5 Me PA Itn C t'� Q Source(culture boartll: C6�L�i �•�U
4 /tt q/ h ,..7 O 1 �� Replicate a 1 2 3 j 4 5 6 7 8/(99110`11 12
• 3 Graa.g Yy ...I...7 e 11443 Culture board cup n'p V1'L1,lit'11n,ya 511Jk 11 1- S
2 5?amt.'', •fet(a 2z441'1, Date and lime organisms were horn at.-p>\t.s OSS"'1 1fx0
• 1-Control between:
Average transfer volume: <0.25 ml
Transfer bowl Information: pH 15.0 1 ..1•'1ry
I Temperature CC) ta�•Q
Daily Renewal Information:
Test initiation,rer,en,al,',meting or Sele�nrem YWr location Randummng
Day Date termination MNSW Batch
Time Analyst eaten Retch Meueater/Shen remolete
111 ua•n/r..ana
0 06.14-23 A 1�'�Z iQu'Ol'LSb 4c.•L41:5 Q'S'S1'ti1 1..-'C.1--
1 06-15-23 r 26
2 06-16-23 e•6 A`ant e.
111 3 06-17.23 r`0 WI kV'
4 Ds 1g-z3 `�'`SpACC
5 06-19-23 a''o;f a 1g d.-oii•u6
ill 6 06-20.23 '" V3S �a\\rT'!"
r<,m,naevnl I
7 06-21-23 O�s� (`t
-Ortanitmt led daily 50 ul NC leienortrum and SO bl per renlirne using srandvSteo,.omit marmot Sri 17E59354
ill Chemical Analyses: initiation Renewal One Renewal Two
Initial Final Initial FinalIn$lal final
Concentration Analyst at A 81_ 1/(_ 8 L V s 6z 01_ (i l_
pH(S,u1 7.?s��,/ra
,30) 7.Y5 '7.70 ?,R2 7..7s 7.'IA
I Dissolved oxygen(mail) 'Y` /3j 1
,`�3 . �'� l.la
Conductivity{µmhos/<ml 303
Control,MHSW •Alkalinity(mei CaCCLI to\ v‘gt6t014
`Hardness Imgh Cam,/ a�'Temperature(%) Vs.1 1S a l Le1.1 2S_\ 'LA.1.. ZS.O
nelyt identified for oath day veil.J pit Mssolord oxygen 005,ndwtn09 mcasvremots Only T.-operator.perfamw-d at Ice limo of lost.anauan.rental or termananan by the
analyst ilennf ed u the Dait,Rrneoal Ida-ma:on table Atka:wry and hardness performed by don=Amu mtrwled no do tut bench thews and transcribed to dos bench sheet
A parameter Reportingltroit Matted number Mete, Serialeumbe,
Po 0.1 S.U. SM a500-H•9-1011 Auumer AR20 91112452
Dissolved oxygen 1.0 mg/L SM a5OO-O 0-2019 YSl Model 510E 111010a324
Conductivity 14.9 pm5ns/on 5M 25108-1011 Auumet 020 923I2452
11 Hardness ity 5.0 mg CBCOI/l SM 23209-2011 Acwmet AR2O 91313452
Hardn SO mg CJC0d1 SM 2340 C-2011 Nor aophob:r Not aoplmble
rums,•vduW d,bv,. 0.1 eng/t ORION 97.70-1977 Actuate(48230 92349/23
Temperature 0.1•C SM 2550e-2010 Digital Thermometer 1hakkA LS
I Survival and Reproduction Data:
Observations Replinte number
Day I 2 3 4 5 ( 6 7 if 9 10 11 12
,
0 2 Adult mortality L L L. L. L. L
Renewer One it=Svc,0-dead) I' tFF•••��� ��--��,,,, i� t �.s �-g,, �.� �'t
Number of broods present LL L L__. L- Li.... L I—, LI, i_
S Number of young produced 11 Pi 11_ VS I (6• , it. is iS t t 15
Renewal Two Adult mortality \_ V_ L L.. S L t_ L- ‘...—
Number of broods present IA_ L. L. IL, L_ IL IL L L IL L I
Number of young produced
Its 1,4 1(e is 13 It % 1 I'L IS 1 1. Ito Ill
7 Total young produced —in •it 1,8 23 21e lb 3` IA ,3p 3\ Z$ 2q
fatal
Final adult mortality L ` L L L L L L• ` I✓ ` V-
I,.•Um,D•de Mt �
X for 3rd Broods X. ..)4 -/T r v- x ([- I `X X
III
Control Acceptance Criteria: of
%of Male Adults(5 20%) 01. Mean Offspring/FemaleJ. .t p••^l
X Adults having 3rd Broods(280%) I 907, offspring/surviving female) o
(2 15 offspring
%Mortality(<205:) 01. %CV(<40%i 5,4 L
6/27/2023, 10:40 AM
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North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0,NC Modification—December 2010,Version 3.0)-Test Bench Sheet
Paired with Control# Z Date: Ot.'14 1.
Client Tryon WWTP NPDES# NC 0021601
Facility Outfall 001
Project a t14Ci` County Polk
Test Concentration(Chronic limit) 37% Dilution mL mL Total volume
preparation! Sample Dilution water mL
Samples were not aerated or treated unless otherwise noted on this form.Control,dilution water 111 189 300
and test renewal information are included on the Control Bench Sheet Indicated above.
Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst aC- A- let. y‘..- 92 VV at- tit eL
pH IS u.) 741 7.0 5 7.8-Ztu 7.45 7 q 1 7 R 5
Test Dissolved oxygen(mg/L) 0,0 CO 49 g•t) -NA -1-1
Concentration Conductivitylµmhos/cml 2g6, 288' 29 7
'Temperature(°Cl 141.b "LS-l 1't.2 1L'1• & 1`1.1 'LS.'L-
pH(5.U.) 7-S a, 7.78'
Dissolved oxygen(mg/L) �,o +,63
r
100% Conductivity(pmhos/cm) 2 to 2.73
'Total residual chlorine(mg/L) G a.II) la.1 t) _
Sample number sample t L30fo i s,It sample 2 oV1S-0-L
'Analyst identified for each day.perfumed pH.dissolved oxygen and conductivity measurements only Temperatures performed at the time of test snniatsnn.renewal or
termination by the analyst identified in the Daily Renewal information table located on the Control Bench Sheet Total residual chlorine performed by the analyst identified on
the Total Residual Chlorine Bench Sheet and transcribed to this bench sheet
Survival and Reproduction Dato (performed at rest concentration):
Day Observations Replicate number
1 2 3 4 5 6 '7 8 9 10 11 12
2 Adult mortality t 1_ l_ L
Renewal One it=Live,D=dead) � �,, ``ii 12.._. �'''� ''.,� ''`` L. '''
Number of broods present <✓ L . L L L=l L L_ L i, L.. L
5 Number of young produced L S t-1 t g 1 S ti I S Ii t1 tl Ii Iq t'L
t
Renewal Two Adult mortality L L L L L- L 1_ l— t` L L.
IL=Live.0=dead) `
Number of broods present 1.1 L t 11 0_ I t L L 1 t L. 1. I L L.
Number of young produced
to, IS Ig get I& tic t$ tie tk LS t-� t'
7 Total young produced 3A L D'1 SS 31 33 31 33 3S 34 36 No
Final
Final adult mortality L. L L. L L L L. L L
IL a Live.D a dead)
Test was initiated using Sample 1.Sample 2 was used for Renewals One(day 2)and Two Iday SI.Samples were diluted to the test concentration prior to use with moderately
hard synthetic water and warmed to 25.0 a 1.0°C in a warm water bath.
Comments:
Test Results and Statistical Analyses:
Test results Statistics
%Mortality O.f t-Stat or _/1 �7
Rank Sum J 7
Mean offspring 1-Tailed 7y�
per female Critical St0"
%Reduction mat 32i PASS or FAIL Ol( 5
from control 1
SOP AT12-Revision 4-Exhibit AT12.2
5 of 10 6/27/2023, 10:40 AM
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Fnvuonmens�l Testing Solutions,In..
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1002.0)
Species: Ceriodaphnia dubia
tlk,W
vrweJ Lv
Facility: Tryon W WTP
Test start date: June 14,2023 .
Project number: 17991 .__
Sample numbers; 230613.11,230615.02
Survival Average Coefficient of Percent reduction from
Number of young produced by replicate number (%) reproduction variation l control I%f
Concentration Day 8 9 10 11 ]2 (offspring/female)
l
(%) 1 2 3 4 5 6 7 -
5 11 14 12 13 13 18 14 16 15 15 12 15
Control —
7 16 14 16 15 13 12 17 12 — 15 16 16 14 100 28.7 5.4 Not applicable
Total 27 28 28 28 26 30 31 28 30 31 28 29
S 15 17 18 18 19 15 19 17 17 19 19 17
37% 7 19 15 19 17 18 18 I 18 16 18 15 17 19 100 34.9 5.0 -21.8
Total 34 32 37 35 37 33 37 33 35 34 36 36 i
Dunnett's MSD value: 1.685 MSD= Minimum Significant Difference
PMSD: 5.9 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10"percentile) =13%.
Upper PMSD bound determined by USEPA(90'h percentile)=47%.
Lower and upper PMSD bounds were determined from the 10"'and 90"'percentile,respectively,of PMSD data from EPA's WET Interlaboratory
Variability Study(USEPA,2001a;USEPA,2001b). The lower PMSD bound represents a practical limit to the sensitivity of the test method and is
not a minimum acceptance criteria.
USEPA. 2.001a,2001b Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-8-01-004 and EPA-821-8-01-005.US
Environmental Protection Agency,Cincinnati,OH.
CT
IJ
v
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O
IJ
W
O
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a

114144,■
Statistical Analyses
4.Mcm.eanal Ir.,1.Wa.Mc V
Cedodaphnia Survival and Reproduction Test-Reproduction
Start Date: 6/14/2023 Test ID: CdPFCRNC Sample ID: Tryon
End Date: 6/21/2023 Lab ID'. ETS-Envir.Testing Sol. Sample Type- DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02.013 Test Species: CD-Cenodaphnia dubia
Comments:
Conc-Y. 1 2 3 4 6 6 7 B B 10
0-Control 27 000 28.000 28.000 28.000 26.000 30.000 31.000 28.000 30.000 31.000
D-Control 28.000 29.000
37 34-000 32.000 37.000 35.000 37.000 33.000 37.000 33.000 35.000 34.000
37 38.000 36.000
Transform:Untransformed 1-Tailed Isotonic
Cone-% Mean N-Mean Mean Min Max CV% N tStat Critical MSD Mean N-Mean
0-Control 28.687 1.0000 28 667 26.000 31.000 5.431 12 31.792 1.0000
37 34.917 1.2180 34.917 32.000 37.000 4.954 12 -9.303 2.508 1.685 31.792 1-0000
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-W Ik's Test indicates normal distribution(p>0.01) 0.93691 0.884 -0.0643 -1.0378
F-Test indicates equal variances(p>0.73) 1.23438 5.31963
Hypothesis Test(1-tall,0.01) MSDu MSDp MSB MSE F-Prob dt
Homoscedastic t Test indicates no significant differences 1.68523 0.05879 234.375 2.70833 4.4E-09 1,22
Treatments vs D-Control
Linear Interpolation(200 Resampies)
Point % SD 98%.CL Skew
IC05 >37
IC10 >37
IC15 >37 1.0
IC20 >37 0.9
IC25 >37 0.8
IC40 >37 0.7
1050 >37 0.6
• 0.5
C 0.4
0
n 0.3
¢ 0.2
0.1
•
-0.1 .
-02 --
0 10 20 30 40
Dose%
Dose-Response Plot
4o
35
30 1-tail,0.01 level
0 25` of significance
•
0 20
J?
i.
15
1D
5
0
3
0
0
0
7 of 10 6/27/2023, 10:40 AM

taw '""a 351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fa,i: [phone]
Environmental Testing Solutions,Inc
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Tryon WWTP NPDES8: iiC0021601 Pipe 8: 001 County: Polk
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution. 37%
Test type. Pass/Fail Chronic Parameter code: TGP3B
1
z
1 Sample information: (to be completed by sample collector)
Composite sample: Sample location: .,u Li
Start date: L lI .i2` . Time: 0�()0 Volume collected fot'testing-
0G Number of containers filled for testi'
3 n I
End dale: /pj)3 j�3 Time: y i8/
99¢ Number of samples per hour: Method of transport to laboratory: 1'SJiGC
Chilled during collection? le"" Comments:
1 If chilled,specify temperature: t•-o
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in icc. The sample must be<6.0°C upon receipt at the laboratory.
i
S
Sample custody: (to be completed by sample collector and facility personnel)
i
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE 1,
Sample collected by:
A,
>)nr Sic.L,t3 1I7C1tI off: cs gO[i
Pom Slams. (1,e sul.me
Relinquished by: Received by.
4/,3! .23 _ b r3� 3
•
nu sty:...,..Jl u:rw• an;s,1,�., m>!t.r o e 3 -i
Pew Syaxue fbia arclume Prui4 ue uN umc
I Relinquished by: Received by:
r 6-r 3 .3 6 11311.
I, KPu tvi c-(0 h;4z .A c/(4 r21:?ca ate)1I Tirre%I Od.i..z- 1! -��
Poet % /5. . care sod Mc P. Sgm+�a th,<nd wa
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
/I$f1-3 0t.•VS ern
ttimr 4ymm.� mien..new Pmr sa,uv.
Custody seals intact?: =I ED CO Sample temperature upon receipt at ETS(°C): r
Y.. et. Na used I I.S
Samples received in good condition?:
Yrs No Total residual chlorine upon receipt at ETS: r1 4
.00
IDPD Presence/Absense Indicator,NIDL..0 t0 ma/t.) r`"" "`Y a... "I,."
06 ola
Tracking number. /I A :f
Project aumber�"ta 1 Sample number: Jwa+'
Comments:
•
8 of 10 6/27/2023, 10:40 AM

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_ 's' 4 "2♦ 4
351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Tryon WWTP NPDES#: NC0021601 Pipe it: 001 County: Polk
Purchase order
Species: Ceriodapftnie dubia Effluent dilution 37%
Test type: Pass/Fail Chronic Parameter code: TGP3B
lSample information: (to be completed by sample collector) �f i
1 Composite sample: Sample location:.eu i.Jct,R ��2.. /Ct�fccI
Start date: 4s-/1- 23 Time: G1C1Ll Volume collected for testing: ¢ccw- -- tl
1 End date: 4,- -a g Time: G c!>C Number of containers filled for telling: in
I Number of samples per hour: Method of transport to laboratory: Odd! ,c t/t/1 1
1 Chilled during collection? t��.a Comments: ±
If chilled,specify temperature: / a 4
jTriple rinse sample container with sample before filling. Completely fill the sample container with no air space.
1 Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
1
1 Sample custody: Ito be completed by sample collector and facility personnel)
1
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
( Sample collected by:
4'i$ I
1 D-f6tcrn/-e. ()44. 4/2,). 0 0 i
l d Swans. I>W an1,
Relinquished by: Received by: )
4,-/r-13 I� �) 0i3/13
D. .tit/{-- I • - P1�= �aai of (E) ee oy. —
It.+ S4epur. Dv.W teat. Ma 'w04.,c
Reliegvi.hsl by: Received hy:
1 11===1= 1\
Pram rwuur. rn,,ad w,c Prr s,pa,ee Oats.ad ue¢
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
6/A-5/eq, t16•ti•L1
. k1417,-e.,_leil 0_1032_'W.2.2ss__ r 0-
woo
Para 34 o,w• Div al tease Ww On..ulbu.
Custody scab intact': =I n i Sample temperature upon receipt at ETS(°C): I L
Tea Na u..l •�
Samples received in good condition?:
Te. N. Total residual chlorine upon receipt at ETS: 1-1 I-7i
riftIDPD Prescnc M °.r, 4
U lbscnse Indicator.MIX...0 10 mlyiL I
Tracking number: rift
Project number: %T•��tCI I Sample number: 90()Dj .0`
Comments:
9 of 10 6/27/2023, 10:40 AM
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• °'= Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
Em,Nnnm.ntal Testing Solutions.Inc. Source: In-house Culture
1.14 1 I 1 I I I I I I I I I I I I I I I I I
1.12 - Control Limits(± 2 Standard Deviations)•
-
•
• •
• •
1.08 — • ,.o _ __ _� — �_ • w n.—• w v a® r.
1.06 - -
co 1.04 -
Z -
1.02 -
I I l I I I I I 1 I I I I 1 I I I I I 1
- 1 1 1 1 1 1 1 l T 1 1 1 1 1 1 1 1 I 1 1 -
u_
>. 1.15 -
Warning Limits
n _.
1.10 - .__..:..s... .. ...._ __ ..—.._..-•,.s..__..__..__..... •.. . -
• • . _•.s�. .•�
1.05 -
1.00 -
0.95
oloalo Ag OS QV 05 .063-01;yo�ti10 oti1O1.y�a014 11:pte 8��zr10:•%,0"01'O 01' pfro dr sf3r Ob
06'13
Test date
• 7-day IC25=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodaphnio reproduction(calculated using ToxCalc).
Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
——.— Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
—..._s. Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
USEPA Warning Limits (mean logarithmic ICZS t 5A 2U converted to anti-logarithmic values,
SAID=10"percentile of CVs reported nationally by USEPA)
10 of 10 6/27/2023, 10:40 AM

r 71
'. PO Box 7565
Asheville, NC 28802
Environmental T,tlM Soluoom.Ine Phone: [phone]
Fax: [phone]
June 22,2023
Ms. Deborah Bradley
Town of Tryon
423 North Trade Street
Tryon, NC 28782
ETS Project Number: 17991
Test Start Date: June 14,2023
Facility Tested: Tryon WWTP
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Parameter Result
Test Procedure, Method Number
Code PASS FAIL
TGP3B North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure v
EPA-821-R-02-013,Method 1002.0 A '
All toxicity testing results required as part of your permit must be entered on the Effluent Discharge
Monitoring Form(MR-1)for the month in which it was performed, using the parameter code TGP3B.
Additionally,the DMR Reporting Form AT-1 must be signed and e-mailed or mailed to the following
address:
ATForms.ATB(ancdenr.gov
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
Please call if you have any questions concerning these results.
Sincerely,
im Sumner
Laboratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
2 of l0 6/27/2023, 10:40 AM

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PO Box 7565
Asheville,NC 28802
Ntonnwna Teasq� Phone: [phone]
Fax: [phone]
September 20, 2023
Ms.Deborah Bradley
Town of Tryon
423 North Trade Street
Tryon, NC 28782
ETS Project Number: 18976
Test Start Date: September 06,2023
Facility Tested: Tryon WWTP
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Parameter Result
Test Procedure, Method Number
Code PASS FAIL
TGP3B North Carolina Ceriodaphnio Chronic Effluent Toxicity Procedure V .
EPA-821-R-02-013,Method 1002.0 /�
All toxicity testing results required as part of your permit must be entered on the Effluent Discharge
Monitoring Form(MR-1)for the month in which it was performed, using the parameter code TGP3B.
Additionally, the DMR Reporting Form AT-1 must be signed and e-mailed or mailed to the following
address:
ATForms.ATB(cr�ncdenr.ciov
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh,NC 27699-1621
Please call if you have any questions concerning these results.
Sincerely,
Jim S mner
Laboratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act. 99053-001
2 of 10 9/25/2023,8:25 AM

PO Box 7565
Asheville,NC 28802
Phone: [phone]
f'~~°^^'°•""'<s°n9'°'°bam•'^4 Fax: [phone]
Effluent Toxicity Report Form-Chronic Pass/Fail and Acute LCsa Date:September 20,2023
Facility: Town of Tryon NPDES#: NC 0021601 Pipe#: 001 County: Polk
Tryon WWTP
Laboratory Performing Test: Environmental Testi Solutions,Inc.,Certificate#037 Comments
Signature of Operator in Responsible` Charge ORC) A, y [ ,�A
ORC Phone/E-mail: deJ t,t,�,L pk (til 14 j1-YL1i2, ',(��j l Project a: 18976
Signature of Laboratory Supervisor: c��� b.. Sample 8: 230905.11,230907.04
e-Mail to: [e-mail]
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-Scat/Rank Sum -2.196
Raleigh,NC 27699-1621 1-Tailed Critical 2.508
%Reduction: -8.1
Percent Average
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Mortality Reproduction
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Control Control
Number of Young Produced 34 31 34 31 28 29 33 31 33 29 30 29 0.0 31.0
Adult Survival: (L)ive,(D)ead L L LLLL L L L L L L Treatment 2 Treatment 2
0.0 33.5
Effluent Percentage 37% Control CV
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 6.7 PASS FAIL
Number of Young Produced 34 37 35 35 33 31 34 31 36 30 33 33 ""'°°'°""•oad'""°'"-"
]M b
Adult Survival: (L)ive,(D)ead L L ILL L L L L L L L 100.0 A
pH(5.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date: September 06,2023
Control 7.57 7.93 7.67 7.93 '7.74 7.90
Treatment 2 7.88 7.94 7.90 8.03 7.97 7.93 Collection(Start)Date:
a 1 Sample 1 09-04-23 Sample 2 09-06-23
� F.W � W N
D.O.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control ' 7.6 7.8 7.7 7.5 7.6 7.6 Grab Comp.Duration
Treatment 2 7.7 8.0 8.1 7.8 7.7 7.8 Sample 1 - X 24-1' `g a , a
Sample 2 . X 24.h = 3 E E
0 A
JI N
LCso/Acute Toxicity Test Alkalinity(mg CaCO3/L) 62
(Mortality expressed as%,combining replicates.) Hardness(mg CaCO]/L) 91
Conductivity 1pmhos/cm) 313,305,300 397 396
Concentration(%) Total Residual Chlorine(mg/L) <0.10 <0.10
Mortality(%) Sample Temp.at Receipt 1°C) 3.6 5.8
LC50= Method of Determination
95%Confidence Limits _Trimmed Spearman Karber t 0 .
to Probit ' W
Other: Control
High Conc.
Organism Tested: Duration: pH(S.U.) DO(mg/L)
DWR Report Form AT-1
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Page 1 of
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnio dubia
(EPA-821•R-02-013 Method 1002.0,NC Modification-December 2010,Version 3.0)-Control Bench Sheet
Control S. 3 Date: 09-06-23
Test Grouping Information: Test Organism information:
Facility Project fl Organism Source: In-house Culture
6 — �..1 Age: <24-hours old
5 Source(culture board): 0 a•15'' A 4-1A-1S1
4 �1k Replicate 61 12 13 a ) 8 9 101 11 12
3 LARCINeSn'su . %&'lt.1 Culture board cup0 Sds)?31 e"i 1' PI16114IS
2 •Tylr*1 p tJ t Q•d1110 Date and time organisms were bom Q 1‘,.O V•1J Q(eoo TIP IIbj
1-Control between:
Average transfer volume: <0.25 ml
Transfer bowl Information: pH(5 u.) 1.e5
. k
Doily Renewal Information: Temperature rt:1 Z1.k
-Tart tNibtlon.renewal.'feed'ng at Selenaavum rwr to<xmn Aandomh,ne
oar Date eamnaGo MNSW eat<h
Time Analyst Batch Batch In<oBatoriSholl remptate
0 09-06-23 "moot 1.4 dne
tab 01•drtl of•St >S Ot•311.., 1 Get
1 09-07.23 r nf.6
dFed
2 09-08-23 `no�tf+�3 at-OS•t,
et
3 09-09-23 v o1sS
a 03.10.23 ..'"'"til`I0
ki
5 09-11-23 a `e"
'WO 4L}
s o9-u Lea
-z3 olelll
7 09-13-23 7 pl1'fb \i\
•0,e amsmt Fed char 50 ut NC Sienat nun,and 501 \rr eel tmtlu to using Handy.t.u.eoest o,Pettor SN:)r593Si
Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Anatyst BL aL 151- a•- ea. BL
pH iSsi I 7.5 7 7.613 7.4,7 7.11 7...Zit .7.11(0Dissolved oayeen Img/ll �.ta 1-6 -7- 'S -101 4-..l e1
CanductlNlty(*mhos/<m) 313 365 - 3 00
Control,MHSW
•Alkalinity(mg/ICaCO3) V1 •el
'Hardness(mg/ICaCC:1 �` G O•�
•Temperature(•C) L'•' AS- 1 1e(.to •t.s-0 IA.O u-o
•Aoalisr Idom&d fos...oh dot.performed PH.dinolsel oe>gen and cwdotut Ity tr octant-nts vole Tcmpootau performed at du OM<of tut ttittauem tau,al or ramnut on e,•the
analyst identified to:he Daily Retenal Infornauoe table Al5.tivttr and hardness performed to the analysts mdenufed on Neat bench sheets ami traosmlxd:o Jos beach shwa
Panmrer i°epor<ina limit Method number Meter Serial number
pH 0.15 U. SM 4500.H•11-2011 Ammer ARID 93312.5:
Dissolved e.ygto 1.0 mg/0 SM 4500-0 G-2016 YSI Model S2Cf -100104324
Conduawiry 149;smhosfen SM 2510 B.2011 Recdmet an20 93112,52
Pakanntry 5 O m64CO,A SM ale B-20ta acwn..r r.91a 91111452
-4Hardness 5.0 mg CaCOall SM 23a0 C-io11 Not appiicabi Not aeel.aolr
Oars named dt.nn. 0 1 mg/1. ORION 97-20-1977 Resumer All250 92349123
Temperature 0t•C 5M23S08-2010 Dtgaatmhermameter AOlel,.4tp/S
Survival and Reproduction Data:
Observations Replicate number
Day 1 2 3 4 5 6 7 8 9 I 10 11 12
2 Adult mortality L_ L L L L t_ L L L L
Renews;Orr. a=We,0.4e401 ' � T
Number of broods present L LL 11.. Li L l.a L L L2 L
14 I1 IL, tS
1'1 I 11- Ito 11 t1.
5 Number of young produced _11 15 •
RenewalTvro Adult mortality t L....- L.. L.- I.- L L
i1 ,1 L L L L L,
a..6.e.0 c dead) ) I' �'t-
Number of broods present Ll L L L L L_ LL LL. t
Numhet of young produced t 1
IL 11 I it., vs 16 iS Iq (a 1 I
7 Total young produced 3a S1 bj.( 3l -Li -In
111. �� 3',-133 let SO 19
Final adult mortality L. k— k— L L- L. t` % `.
It•1.e,0•deaca ``," �,
X for 3rd Broods 7& X )6.. x % >r— X. X... $ X
Control Acceptance Criteria:
%of Male Adults(5 20%) 01. Mean Offspring/Female%Adults having 3rd Broods(?80%) (37 (2 15 offsprng/surnving Tema e) 3 �l1•v
x MortaNty(e 20=) 0 C. %CV I<4O%) (0.1 7
4 of ID 9/25/2023, 8:25 AM

Page l of.
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubio
(EPA-821-R-02-013 Method 1002.0,NC Modification-December 2010,Version 3.0)-Test Bench Sheet
Paired with Control It 3 Date: Oq•06-"13
Client Tryon WWTP NPDES# NC 0021601
Facility . Outfall 001
Project# Vi11.0 County Polk
Test Concentration(Chronic Limit) 37% Dilution ml mL Total volume
preparation: Sample Dilution water mi.
Samples were not aerated or treated unless otherwise noted on this form.Control,dilution water 111 189 300
and test renewal information are included on the Control Bench Sheet indicated above.
Chemical Analyses: Initiation Renewal One Renewal Two
4
Initial Final Initial Final Initial Final
Concentration Analyst et_ ©L 13L et-
pH(S.U,I 7.SV 7.It? 7.40 s.o3 7.0-7 7.43
Test Dissolved oxygen(mg/L) "1e.Q 8.1 67 I!lull Concentration Conductivity(pmhos/cml 350 341, 314'0
'Temperature(°C) 'LS,'l Z,�-q ' .`1 -Ls_t t�19 -Ls-U
pH(S.U.) 7•irto 12
Dissolved oxygen(mg/L) 1,"1
100% Conductivitylpmhos/cm; v77 39G
'Total residual chlorine(erg/I) G D, 1 D L O.1 D
Sample number sample 1 1_'Of.OS I I sample 2 -`-2 Dot •a�
-Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures performed at the time of lest initiation.renewal or
tennmatmn by the analyst identified in the Daily Renewal Information table located on the Conuol Bench Sled Total residual chlorine performed by the analyst identified on
the Total Residual Chlorine Bench Shoot and transcribed to this bench sheet
Survival and Reproduction Data (performed at test concentration):
Day Observations Replicate number
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality ` L ` L- lr L L
Renewal One (L=live,D=dead)
Number of broods present L� L L� L= E L.= LL L1
5 Number of young produced IS 'Lk (q tom] It It IS 19 11 t-t 20 Ig
Renewal Two Adult mortality L ' L L ` L V_ U L
(L=Lrve D=dead) t`
Number of broods present L l� L1 L l 1 Lt._ LL L I, ILL L L
Number of young produced 1 4 I(a Iles I& t S t 3 t 11- %S 13 I'S 1 S
7 Total young produced
Final 3a 31 3S 35 33 3l 3`i 31 36 30 n S9
Final adult mortality U L ` ` L
(L=Live,D=dead)
Test was initiated using Sample I.Sample 2 was used for Renewals One(day 2)and Two(day SI Samples were diluted to the test concentration prior to use with moderately
herd synthetic water and warmed to 25.0 S 1.0°C in a warm water bath
Comments:
Test Results and Statistical Analyses:
Test results Statistics
%Mortality //) t-Stat or _
v7' Rank Sum 2')9b
Mean offspring 1-Tailed per female 33-S Critical 1-S 0 e
%Reduction - 8 t L_ PASS or FAIL PASS
from control
SOP AT 12.Revition 4-Exhibit AT12.2
5 of 10 9/25/2023,8:25 AM

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[mitonmMulUtlin9 Solutions,Inc.
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1002.0)
Species: Ceriodaphnia dubia
Facility: Tryon WWTP _ .',L�• ..vY�
Test start date: September 06,2023 ,�'',
Project number: 18976 a,
Sample numbers: 230905.11,230907.04
Number of young produced by replicate number Survival Average Coefficient of Percent reduction from
Concentration (%) reproduction variation(%) control(%)
(%) Day
1 2 3 4 5 6 7 8 9 30 11 12 (oltspringJlemalel
I l
5 17 15 17 13 12 16 17 16 14 11 16 15
Control 7 17 16 17 18 16 13 16 15 19 18 14 14 100 31.0 6.7 Not applicable
Total 34 31 34 31 28 29 33 31 33 29 30 29
5 15 21 19 17 18 18 15 19 21 17 20 18
37% 7 19 16 16 18 1S 13 19 12 15 13 13 15 100 33.5 6.3 -8.1
Total 34 37 35 35 33 31 34 31 36 30 33 33
Dunnett's MSD value: 2.150 MSD= Minimum Significant Difference
PMSD: 6.9 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(SO'"percentile) =13%.
Upper PMSD bound determined by USEPA(90"percentile)=47%.
Lower and upper PMSD bounds were determined from the 10'"and 90'°percentile,respectively,of PMSD data from EPA's WET Interlaboratory
Variability Study(USEPA,2001a;USEPA,2001b). The lower PMSD bound represents a practical limit to the sensitivity of the test method and is
riot a minimum acceptance criteria.
USEPA+A3:1131. 2001a,200lb.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-8.01-004 and EPA-821-B-01-005.
US Environmental Protection Agency,Cincinnati,Of-I.
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/Emceed he:
Reece Keenan
Statistical Analyses "°midn"
Enwrennwe W Twang Sohnionc Inc t
Ceriodaphnia Survival and Reproduction Test-Reproduction
Start Date: 9/6/2023 Test ID: CdPFCRNC Sample ID: Tryon
End Date: 9/13/2023 Lab ID: ETS-Envir Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
Comments.
Conc-% 1 2 3 4 5 6 7 8 9 10
0-Control 34.000 31.000 34.000 31.000 28.000 29.000 33.000 31.000 33.000 29.000
D-Control 30.000 29.000
37 34.000 37.000 35.000 35.000 33.000 31.000 34.000 31.000 36.000 30.000
37 33.000 33.000
Transform:Untransformed 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
0-Control 31.000 1.0000 31.000 28.000 34.000 6.738 12
37 33.500 1.0806 33.500 30.000 37.000 6.300 12 -2.916 2.508 2.150
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution(p>0.01) 0-95805 0.884 0.04929 -1.0485
F-Test indicates equal variances(p=0.97) 1.02083 5.31963
Hypothesis Test(1-tail,0.01) MSDu MSDp MSB MSE F-Prob df
Homoscedastic t Test indicates no significant differences 2.15022 0.06936 37.5 4.40909 0.008 1,22
Treatments vs D-Control
Dose-Response Plot
40
35
30• - 1-tail.0.01 level
of significance
o 25
u
a 20
0
`0.
15
10
5
0
tn
2
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=' . 35l Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility Tryon WWTP NPDES#: NC0021.601 Pipe II: 001 County: Polk
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 37%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Composite sample: Sample location: .afe,/Vett Jiz ..s,.(dcroil
Start date: q-N- ).3 Time: cc*g4,76 Volume collected for testing: C`
End date: 9-,y-3 3 Time: c g00 Number of containers filled for testing: I
Number of samples per hour: 4./ Method of transport to laboratory .k{ACe.
Chilled during collection? Comments:
if chilled,specify temperature: lytt
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample collected by: ,?
0-5-73
17:i'CO MCV 1 niey
/4D Svc)
tow SWmae da eel w°o
tRelinquished by: �/G�/g� G/ Received by:
v4J :�LGi naty y /r�Y ok 0 kR,lvt C-l�ht o,.,ez;l'-//4r c 04s i
Pmt s ave is a me to Pw spume IAia oat unto
Relinquished by: Received by:
y—r-.23 / 4'/V 23
ILP.obvc..u)ki* �""� c.lg& Its, ( t�.11 �it�,Gl�\ 2 lia`i
Ivwt `Wtv. Oua andu°i. Punt Sipatve D.and tone
(er..._. . _ _ _.x_-a..mr-,,..r.:. ..-:�c�., —..---._-----
Sample receipt Information: (to be completed by ETS personnel)
I Relinquished to ETS by: Received at ETS by:
� 9/17. 3 0t1 OS2.3
p l w l el( I Iff- .>0.12. 13`-- 11 1A- 1A- r 300
Wee s,.,ma. uanM w. Prom Stm.tm< rhm oat one
Custody seals intact?: [J [ © Sample temperature upon receipt at ETS(°C): /' L
ya. N., Natun] 1,0.
Samples received in good condition?: [J n r_�
r.s N. Total residual chlorine upon receipt at ETS: 1__ i
IDPD PresencelAbsense Indicator,MDL a 0 10 me-) WOptl Ab"'
Tracking number: f of"J p 9�
Project numberf Vp 1� ,t.X�p Sample number: OS.II
Comments:
8 of 10 9/25/2023,8:25 AM

imam
351 Depot Street
Asheville,NC 2880I
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Tryon WWTP NPDES k. NC0021601 Pipe d: 001 County: Polk
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 37%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Composite sample: Sample location: [1Je2, i3e�0y�,
Stan date: 7-4, -1� Time: O)O Volume collected f resting: /
End date: Cj-7- a I Time: d ) Number of containers filled for testing/ �
Number of samples per hour: jf- Method of transport to laboratory Q dt/j /LE
Chilled during collection? gip„ Comments:
If chilled,specify temperature: Lf '
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample collected by:
mcorvi
14iw topuwe Dare led nmc
Relinquished by: Received by:
4-7.25
raw �
44,43,1
'n• Oamwltmc Hut D,c ealrun<
Relinquished by: Received by:
- i
Peen Simeon Dwemfine red Spnr.. ram.w w.
Sample receipt information: (to be completed by ETS personnel) ^ {�
Relinquished to ETS by: Received at ETS by:
Omelet Owe/ if 111(� �1 o
nry Srpaty fh+c anJ emc Ifinr 4rmnune Dew:ut wm
Custody seals intact?: =I =I © Sample temperature upon receipt at ETS(°C):
Yu No Na e.al 5.8_Samples received in good condition?: n n e
Y. N. Total residual chlorine upon receipt at ETS: Q 1=1/
t (DPD Presence&Absense indicator,MDL=0 10 mgiL)
Tracking number: f kT '
Project number:l 1 Sample number:9lJI9(4i.4
Comments:
9 of 10 9/25/2023,8:25 AM

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• ri air dmCeriodaphnia dubia
Chronic Reference Toxicant Control h C art
E Source: In-house Culture
nvlfpmm•nOl T•stllp Solutions,Inc.
1.14
1.12 - Control Limits i(± 2 Standard Deviations)
-tons)
1.10 - • • • •
• •
1.08 - • — ... >• • •
1.06 - -
co 1.04 - -
z
1.02 - -
1 I I I I I I I Irsi ! I I I 1 I
UI
- I t 1 1 I I I I r I - I I r I I I I I I _
>. 1.15 - -
Warning Limits
n
1.10 — s". . :..�..,..+...�..,..,..,.. _..._�. �•���.• • ....
1.05 -
1.00 - -
0.95 I I I I I r I I I I 1 I t 1
Oy_f,Q503 160�111 1ti 0 0114.13".0�11 051i OB 1LA6 11V10'0,1.01 0.01 lPOs oSO11�06 mi IV, 1 11:
Test date
• 7-day ICZS=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodaphnia reproduction(calculated using TozCaic).
Central Tendency(mean logarithmic ICz5 converted to anti-logarithmic values)
—• - -• Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
•--.•.--• Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
USEPA Warning Limits (mean logarithmic IC,±Ski,converted to anti-logarithmic values,
sA la=10th percentile of CVs reported nationally by USEPA)
Yirw,
10 of 10 9/25/2023, 8:25 AM

PO Box 7565
Asheville,NC 28802
Phone: [phone]
bMmmnoxilt tleq Wuhan.Inc
Fax: [phone]
December 20,2023
Ms. Deborah Bradley
Town of Tryon
423 North Trade Street
Tryon,NC 28782
ETS Project Number: 19249
Test Start Date: December 13,2023
Facility Tested: Tryon WWTP
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Parameter Result
Test Procedure,Method Number
Code PASS FAIL
TGP3B North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure y
EPA-821-R-02-013,Method 1002.0 A
All toxicity testing results required as part of your permit must be entered on the Effluent Discharge
Monitoring Form(MR-1)for the month in which it was performed,using the parameter code TGP3B.
Additionally,the DMR Reporting Form AT-1 must be signed and e-mailed or mailed to the following
address:
ATF o rm s.ATB Cad n cde n r.g ov
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh,NC 27699-1621
Please call if you have any questions concerning these results.
Sincerely,
OA
Jim umner
Laboratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
2 of 10 1/3/2024,8:02 AM

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PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
Effluent Toxicity Report Form-Chronic Pass/Fail and Acute LCS0 Date: December 20,2023
Facility: Town of Tryon NPDES#: NC 0021601 Pipe#: 001 County: Polk
Tryon WWTP
Laboratory Performing Test: Environmental Testing Solutions,Inc.,Certificate#037 Comments
Signature of Operator in Responsible Charge(ORC)c' j.&�.ao.A 7.1e4A f
ORC Phone/E-mail CI i6/7A-�)T cltl!/E c) (1 I-iyQd•• e�'JYL Project it 19249
Signature of Laboratory Supervisor. Sample d: 231212.07,231214.02
e-Mail to: ATForms.ATBl lncdenr.gov
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-Stat/Rank Sum -0.567
Raleigh,NC 27699-1621 1-Tailed Critical 2.508
%Reduction: -1.8
Percent Average
North Carolina Ceriodophnia Chronic Pass/Fail Reproduction Toxicity Test Mortality Reproduction
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Control control
Number of Young Produced 31 32 30 34 35 34 37 30 32 30 33 30 0.0 32.3
Adult Survival. (L)ive,(D)ead L L L L L IL L L ILL Treatment 2 Treatment 2
0.0 32.9
Effluent Percentage 37% Control CV
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 7.1 PASS FAIL
38 35 33 31 33 31 35 35 30 28 34 32 "'"°'�",,;,"" °°'�'`
Number of Young Produced =�•^�`"�° x
Adult Survival: (L)ive,(0)ead L L L L L IL L L L L l 100.0
pH(S.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date: December 13,2023
Control 7.76 7.90 7.67 7.94 8.01 7.91
Treatment 2 7.91 7.98 7.91 8.00 7.97 8.04 Collection(Start)Date:
ti i° 2 Sample 1 12-11-23 Sample 2 12-13-23
3 0 r w
D.O.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control 7.7 7.8 7.7 7.7 7.7 7.7 Grab Comp.Duration
Treatment 2 7.8 8.0 7.9 8.0 7.8 7.6 sample I • X 24.1, o
2 E E
Sample 2 X lan 3
LC50/Acute Toxicity Test Alkalinity(mg CaCO,/L) 59
(Mortality expressed as%,combining replicates.) Hardness(mg CaCO,/L) 86
Conductivity(Rmhos/cm) 303,299,301 326 335
Concentration(%) Total Residual Chlorine(mg/l) <0.10 <0.10
Mortality(%) Sample Temp.at Receipt(°C) 2.1 3.1
LC50= Method of Determination
^
95%Confidence Limits _Trimmed Spearman Karber A w ,r, w
to _Probit
Other: Control
High Cont.
Organism Tested: Duration: pH(S.U.) DO(mg/L)
DWR Report Form AT-1
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Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02.013 Method 1002.0,NC Modification-December 2010,Version 3.0)-Control Bench Sheet
Control s: Date. 12-13-23
Test Grouping Information: Test Organism Information: —!—
Facility Project R Organism Source( In-house Culture
6aJtl�e 1gZa{� Age: <24-havers old
5 TR a t,) sit IAA Source(culture board): t•.oCtS•11 A �t-,ab•t ps
4 NL LfiWtt. tgitcs1 Replicate 0 1 2 3 4 516 7 3 9 10111 12
3 14 OtfL e4tSd Culture board cup R1J11Aol4�bl�'�� t y114 13
2 FIRAta1Ua1?I,,wk4CP tco..,1 k Date and time organisms were born 11.•t1,,, OSit TO O'°IO
1-Control between:
Average transfer volume: <0.25 int
Transfer bowl Information: pH(5 t/1. -1,%t-`
Temperature laC)' 1•�•8
Daily Renewal Information:
TNUNSMVon,renewal,•lee dlrtg or Selenaatrum ever Location Rend.minng
n
Dry Date tenlnanon MHSW Batch
Time Analyst Pateh Patch Incubator/Shelf Template
0 12-13-z3 --A: ak ttwt-tbb ti•,$3e1a) .t(1...•i..i i_Cl it-Q
1 12-14.23 731'1 I ?re\
2 12-15-23 roe mace eal+a
tz
41 •ob t�D
3 12-16-23 'Zig 3 8 `j/^
4 12.17.23 `n3.3. PIA\
5 12-18-23 a a, tni.freedje4 k li..01.1..115
6 12.19.23 r`AsRJ' Ak
7 12-20-23 7e,m:•tl1n,
Cltaa (t{
'Organ'tm c led daily 50 uLMCSNenostnrm and 50?1 per rapLute uunr He MvSte p reveal'Wetter Sri 17E59354.
Chemical Analyses: Initiation Renewal One Renewal Two
initial Final Initial Final initial �Fiinal
Concentration Analyst 01 01. Ot- Y t_ ` Q I
pH(S.uI .?ee}{i _ ?-ID 4.6,7- VW 1 -IA%
Dissolved oxygen Imdt) "t 1•1 '\,'1 ,)-- �1L,'}, 1•1
' Conductivity(pmho+/cmi 30 3 e ` 2 ,/f I tJ1
Control,MHSW •AINallnity(mg/I GCO,) S4.
'Hardness lme/L cocoa) bIO 5i. _,%au fi
'Temperature(c) lq•1e tS•\ 1?t.&' ' %S• 0 %4.1 Z`i.9
•Anahn deoNEed for each dal'.performed pH,dt550lynd canal and cond.-snits ncuvrmrnu old)- Tempaaalures perform:A at the hew offal eul;aionn rrnntai or tarwnauon b.de
suiecl Idmtir d ie doe Doh Rosa al Incantation able Ailal tom-and lot dome poufo red b5 the aval,als oldurulled on doe test bench al nt and uwrnbed to Om bosh ahem
Parameter Reporting limit Method number Miter Serial number
P+H 0.15.U. SM 4500.Ma 8-2011 A<timet 4R20 93312452
Oiscolrtd airmen 10 mg/L SM 4500-0 G.2016 YSI Model 52CE 180104324
Conduniss01 14.9 Mmho+/cm SM 25106-2011 oaumrc ARM 93312452
Alkallniry 5.0 mg Ca000. SM 2320B-1011 Aerumet MOO 93312452
Hardness 5.0 mg CaCO•L SM 2340 C-21311 tier apphrable tint appiceble
Tout nrlba<0 roe Cu. me/t ORION 97.70.1977 Atrium el 40150 92349113 TempenHare 0.1•C Sed 2550P3010 Oig,tal Thermometer t 44-4114 S
Survival and Reproduction Data:
Observations Replicate number
Day
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality k_ L \_ ` ` ` \. L L ` ‘..---
Renewaf One (L=th,e,D.deat) s.a �-s
Number of broods present LI L
— - L't- t L LI, L L_ � L_ [3-_, L L_
5 Number of young produced tS 1 05 IS I l 1 b I a 18 IS 11 IS tl llo
R-newal Two Adult mortality 0 l_ \. N-- t-- \— 5 5 L L
‘..--
IL•sue,Da dead) Li �� �� IL ', , 1 T�
Number of broods present L! L Ll L` L1 Li Li Li L' ,�-
Numberofyoungproduced t10 1'• IS 11 I • tt (Ct IS I5 11 IL ILA
7 Total young produced V 3'1- 31) Ns4 35 3`l 31 30 3t 3b 33 , 30
F na' i
Final adult mortality I L tr. L L L t--. L—
(L•li•e,0 a dead} t
K for 3rd Broods X X X X J'-- X )' J". X Y.
Control Acceptance Criteria:
%of Male Adults(5 20%) Q-I. Mean Offspring/Female
%Adults having 3rd Broods le 80%) t 00 1 (2 15 offspring/surviving female) 32- •3
%Mortality 15 20%) Q-) , %CV(<40%) 1, l i,
SOP 4712-Rnitlon a.Eahlba All 3
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North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dutria
(EPA-821-R-02-013 Method 1002.0,NC Modification—December 2010,Version 3.0)-Test Bench Sheet
Paired with Control ti 7- Date: it-15.15
Client Tryon WWTP NPDES II NC 0021601
Facility . Outfall 001
Project R 1 01..44 County Polk
Test Concentration(Chronic Limit) 37% Dilution ml mi. Total volume
preparation: Sample Dilution water mi.
Samples were not aerated or treated unless otherwise noted on this form.Control,dilution water 111 189 300
and test renewal Information are included on the Control Bench Sheet indicated above
Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final ,I,nnitial TI Final//
Concentration Analyst et- et- o L y , V" I Iy
pHIs.U.I 7--91- �/t.98• 1-.9j t2).00 '-9� &•o•I
Test Dissolved oxygen Img/t) e& E.0 1.61 0.0 4.1(2) —1•1D
Concentration Conductivitylymhos/cm) 315 315 , Si
'Temperature(°CI 15.1 1S. o IA•k \.0 •Z- %A.-tf 'NA.i .
pH(S.U.) 7-76, 7.7•1.
Dissolved oxygen(Mg./1.) -1-1 '1.4
100% Conductivity 1pmhos/cm) 326 335
'Total residual chlorine(mg/ll <p.tT) 4 v.10
Sample number Sample 1 'IN 1it1•a • Sample 2 .�,--"4. pt...
'Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only.Temperatures performed at the time of test initiation,renewal or
termination by the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet Total residual chlorine performed by the analyst identified on
the Total Residual Chlorine Bench Sheet and transcribed to this bench sheet
Survival and Reproduction Data (performed of test concentration):
Observations Replicate number
Day 1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality I`. L- >✓ L L ‘.-- L- L
Renewal One (L=live.D=dead) �-� 't r t y '' '.�
Number of broods present I Z Li LE L I . t_ L L L= L Li L`
Iq I Ib ►& IS 1b iL la% ►b Is ti ‘to
5 Number of young produced
Renewal Two Adult mortality !✓ L- ` L 1✓ L- L. L_ L L-
(.=live,D=dead)
Number of broods present L.t L t LL Li L L . L-, -I it_ L` t_. L.
Number of young produced iq t1 iZ tl If I% lg Ili. IJ1 1-5 11 IL.
1 Total young produced 3d SS 3'tb a -5., 31 JS 3S 30 -Lt 34 '%-
Final
Final adult mortality I..... %._ L L A._. ‘.._. L L- L
it.=Live.D=dead)
Test was initiated using Sample 1.Sample 2 was used for Renewals One(day 2)and Two(day 5).Samples were diluted to the test concentration prior to use with moderately
hard synthetic water and warmed to 25.0 e 1.0`C on a warm water bath.
Comments:
Test Results and Statistical Analyses:
Test results Statistics
%Mortality t-Stat or _,�r S
D1' Rank Sum 567
Mean offspring ^ 1-Tailed n
per female A9 Critical 0 b
%Reduction -AQ, / PASS or FAIL PASS
from control J J.
SOP AT 12-Revision 4.Exhibit AT12.2
5 of 10 1/3/2024,8:02 AM

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Environmenu'Testing Solutions,lac
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1002.0) 1
Species: Ceriodaphnia dubia
i fin Mee owl
Facility: Tryon WWTP ae...red nr.
Test start date: December 13,2023 AProject number: 19249
Sample numbers: 231212.07,231214.02
Number of young produced by replicate number Survival Average Coefficient of Percent reduction from
Concentration Day (%) reproduction variation(%) control(%)
(%) 1 2 3 4 5 6 7 8 9 10 11 12 (offspring/female)
5 15 19 15 17 16 18 18 15 17 13 17 16
Control 7 16 13 15 17 19 16 19 15 15 17 16 14 100 32.3 7.1 Not applicable
Total 31 32 30 34 35 34 37 30 32 30 33 30
5 19 18 16 18 15 16 16 19 16 15 17 16
37% 7 19 17 _ 17 13 18 15 19 16 14 13 _ 17 16 100 32.9 8.2 -1.8
Total 38 35 33 31 33 31 35 35 30 28 34 32
Dunnett's MSD value: 2.579 MSD= Minimum Significant Difference
PMSD: 8.0 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD Is the minimum percent difference between the control and treatment that can be declared
statistically significant In a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(109h percentile) =13%.
Upper PMSD bound determined by USEPA(90th percentile)=47%.
Lower and upper PMSD bounds were determined from the 10ih and 90'h percentile,respectively,of PMSD data from EPA's WET Interlaboratory
Variability Study(USEPA,2001a;USEPA,2001b).The lower PMSD bound represents a practical limit to the sensitivity of the test method and is
not a minimum acceptance criteria.
USEPA+A3:R31. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-8.01-004 and EPA-821-8-01-005.
US Environmental Protection Agency,Cincinnati,OH.
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Statistical AnalysesRetri w`d�"
Enwirom,memtl Testing Seh,uont eM.
Ceriodaphnia Survival and Reproduction Test-Reproduction
Start Date: 12/13/2023 Test ID: CdPFCRNC Sample ID: Tryon
End Date: 12/20/2023 Lab ID: ETS-Envir.Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
Comments:
Conc-% 1 2 3 4 5 6 7 8 9 10
0-Control 31.000 32.000 30.000 34.000 35.000 34.000 37.000 30.000 32.000 30.000
D-Control 33.000 30.000
37 38.000 35.000 33.000 31.000 33.000 31.000 35.000 35.000 30.000 28.000
37 34.000 32.000
Transform:Untransformed 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
0-Control 32.333 1.0000 32.333 30.000 37.000 7.142 12
37 32.917 1.0180 32.917 28.000 38.000 8.240 12 -0.567 2.508 2.579
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution(p>0.01) 0.96797 0.884 0.24785 -0.2581
F-Test indicates equal variances(p=0.60) 1.37926 5.31963
Hypothesis Test(1-tall,0.01) MSDu MSDp MSB MSE F-Prob df
Homoscedastic t Test indicates no significant differences 2.57937 0,07977 2.04167 6.3447 0.57628 1,22
Treatments vs D-Control
Dose-Response Plot
40
35
•
1-tail,0.0t level
30- - of significance
0• 25
U
13 20
O
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10
5
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351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Tryon WWTP NPDES N: NC0021601 Pipe k- 001 County: Polk
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 37%
Test type: Pass/Fail Chronic Parameter code TGP3B
9
Sample information: (to be completed by sample collector) `p /
Composite sample: Sample location p 'LA.bJdJJ,. 1(
Start date. t 2(11 3 Time: Zi 114o Volume colleted fo testing: tt
End dale: 1)..I;7 1� fSQU 3 Time: O Number of containers filled for teting: /
Number of samples per hour: Method of transport to laboratory: Ark C (
Chilled during collection? yA") Comments:
if chilled,specify temperature: i it'
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. ,
1
Sample custody: (to be completed by sample collector and Facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE 1
• Sample collected by:
t— 'cod Mail!Mel I `y� ,/ sia71 o vveL)47
Pm ➢waan Oslo and tune
Relinquished by: Received by:
"I fjrOt� IG:M /�/,,� ,r 1��3173 I- I„ ,. i)-/1-:2.
l+'^'d tin NM Larne Pnd &ounce bate and tms
Relinquished by: Received by:o
//evrn C. 1:,,ir.-/,ill., •11 3u 01-e ttT:W�il �.l Fitt �_,:-ct-_ //
Yean bpuhaa 4 aM IsmY Pnd SW.. Date and ewe
Sample receipt information: (to be completed by ETS personnel)
Relinquished 10 ETS by: Received at ETS by:
l• NM s,ywaaee Din and on Ihu: ztweaee Dan mown
Custody seals intact?: =I I= Rre Sample temperature upon receipt at ETS(°C):
Yea Nn Nei
ulc-1—�, 2.t a C
Samples received in good condition?: t� I.�J
y" N" Total residual chlorine upon receipt at ETS:
(DPD Presence/Absee se Indicator.MDL-0 10 mg/t.) "' ^""a"
Tracking number: ili44 q
Project number: 1�114 `Sample number: 11\112-01
Comments:
8 of 10 1/3/2024,8:02 AM

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351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Tryon WWTP NPDES#: NC002160i Pipe lt: 001 County. Polk
• Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 37%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Composite sample: Sample location:. i/;L J{/r am yTfu 'c St)
Start date: i 11 f 3 1 1.3 Time: ( AO U Volume collected fortesting: �w� U
�J
End date )D i4 3 Time: GU C� Number of containers filled for ing: /
Number of samples per hour Method of transport to laboratory: � [/� �;,f/`
Chilled during collection? i/:_;n Comments:
If chilled,specify temperature: J y `C
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
1 Sample collected by: //
// // i:l-t�J-1i
r6-01:1 MG�AA� 7/�rtrt// D�DTJ
nee /lvmr<. Ms atal tow
Relinquished by: Received by:
d ► 09= he„well ��-�>07-e - gs-
rnd 4:4
Pm.
Relinquished by: Received by:
Pm svmwft o..and... e,ao_ ...
sp* Dec a.amx
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received■t ETS by:
CcieJr 4,el1 i, ;I '8 - ,— L (2. 15"
r o15�
S;PAun IA,e andime Prv. Spume pie.°l arts
Custody seals intact?: n n ED Sample temperature upon receipt at ETS(°C):
Yes No Nat 1..1
Samples received in good condition?:
Y. "^ Total residual chlorine upon receipt at ETS: E Mill
(DPD Presence/Absence Indicator,MDL-0 10 man.)
Tracking number: 241 Sample
Project number:1 number: A31 LIlt)'t.OZ
Comments:
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Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
Environmental rouble Solution.,Inc. Source: In-house Culture
1.14
t i t r r i
c r i i r i 1
Control Limits(± 2 Standard Deviations)
1.12 - -
• __ ---- • -- _ -- �.
•
1.10 - • • • • _
• a • • •
7
•
•
1.08 - • • • -
�.
to
z
` 1.06 - _
I
Sn
- i I I I I I I I I I i I I 1 I I I I r I -
1.25 - -
- Warning Limits -
1.20 - _
1.15 - _
1.10 - i . ._. ..,,_..,,,_........_... .....•--•.�..- .,_- ,. .._.. •
1.05 - -
1.00 - -
0.95 1 1 1 1 1 1 1 1 1 l 1 I 1 1 1 1 1 1 1 1
01,11 AOS ti 09 83 s1 11 pt 1
1p4 lZ,pb �10 of p1 03 p1 10 pfk OSAti p6'p61p6A6 01 1�Og pe;,,ti 6i 63 A1 y1 05'LS
Test date
• 7-day IC25=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodaphnia reproduction(calculated using ToxCalc).
Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
--—_— Control Limits (mean logarithmic IC2i±2 standard deviations converted to anti-logarithmic values)
— ••--. Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
USEPA Warning Limits (mean logarithmic IC25±SA.io converted to anti-logarithmic values,
SA 2n=10th percentile of CVs reported nationally by USEPA)
T
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PO Box 7565
Asheville,NC 28802
•
Phone: [phone]
Fax: [phone]
June 21,2022
Ms.Deborah Bradley
Town of Tryon
423 North Trade Street
Tryon,NC 28782
ETS Project Number: 16998
Test Start Date: June 07,2022
Facility Tested: Tryon WWTP
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Parameter
Code Test Procedure,Method Number Result(ChV)
THP6C Fathead minnow, Pimephales promelas,Larval Survival and Growth Chronic
EPA-821-R-02-013,Method 1000.0 >100%
All toxicity testing results required as part of your permit must be entered on the Effluent Discharge
Monitoring Form(MR-1)for the month in which it was performed,using the parameter code THP6C.
Additionally,the DMR Reporting Form AT-5 must be signed and e-mailed or mailed to the following
address:
ATForms.ATB(a.n cdenr.qov
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh,NC 27699-1621
Please call if you have any questions concerning these results.
Sincerely,
Ji Sumner
Laboratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
2 of 14 8/24/2022,7:32 AM

PO Box 7565
Asheville,NC 28802 c s-v t 't
Phone: [phone]
Fax: [phone] e 1�,/112
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date: June 21,2022 Facility:Town of Tryon NPDES It:NC 0021601 Pipe 4: 001 County: Polk t2Ae4„-� �1'iet/�
Tryon WWTP fr
laboratory Performing Test Environmental Testing list s,Inc,Certifica' 4 037 Comment. �o„7a
Cfpf
Signature of Operator in Respo,n1:;Charge C): r�"/
ORC Phone!E-mail: G_7/�_pL M/�2'_ /I,((')i a C.t-W.. Pro m%c 16598
Signature of Laboratory Supervisor: samples: imw6.0s,nor m,12061801
e-Mail to:ATForms.ATBfln nCdenr.Rov Test Organisms:Pimephales promelas
Or Mall Original to: North Carolina Division of Water Resources Supplier
Water Sciences Section/Aquatic Toxicology Branch I In-house Culture
1621 Mail Service Center Beam hatch: 06-06-22 1500
Raleigh,NC 27699-1621 End hatch: 0607.12 0450
Test Start Date: I June 07,2022
Replicate number
1 2 3 4
Control surviving numberolianae 10 10 10 10 Survival(%) 100.0
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.824
weight/onginal(mg/lanan) 0.878 0.779 0.831 0.808 Average vie/ 0.824
snowing(m61
%Effluent surviving number of larvae 10 10 10 10
Lap Original number or larvae 10 10 10 10 Survival(%) 100.0
Weight/orginallmg/lanael 0.696 - 0.827 - 0.802 - 0.748 Avenge wt(mg) 0368
%Effluent Surviving number of lame 10 10 10 10
118S%I Original number of larvae 10 10 10 10 survival l%l 100.0
Weight/original(mg/larvae) 0.694 0.791 0.760 0.860 Avenge on(mg) 0.776
%Effluent Surviving number of larvae 10 10 10 10
I 37% I Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.844 0.765 0.795 0.679 Avenge vat Ong' 0.771
%Effluent surviving number of larvae 10 10 10 10
1 74% I Original number of lawn 10 10 30 10 survival l%) 100.0
weight/orninal(mg/larvnl 0.673 0.718 0.711 0.812 Avenge wtInv) 0.729
%Effluentnumb,of
surviving numbof larvae 9 10 10 10
r
l Original number of lame 10 10 10 10 Survival)%1 97.5
Weight/original(mg/larvae) 0.666 0.806 0.789 0.674 Avenge vrt Imgl 0.734
Water Quality Data
Day° Day1 Day2 Day3 Day4 Day5 Day6
Initial I Final Initial 1 Final Initial 1 Final Initial I Final Initial 1 Final initial 1 Final initial ) Final
Control
pH(SDI: 7.72 7.60 7.59 7.59 7.78 7.65 7.63 7.61 7.64 7.56 7.62 7.58 7.57 7.16
120(mg/U: 7.7 TA 7.7 7.8 7.8 79 7.7 8.0 7.7 7,4 7.7 7A 74 7.3
Temp.CC': 24.8 24.6 24.8 24.8 24.7 24.6 24.7 24.4 I4.3 24.4 24.7 24.6 24.9 24.7
High Concentration
pH 151.11: 6.08 794 799 7.90 8.06 7.93 9.08 7.99 812 7.94 8.10 7.77 8.10 7.51
DO(mat: 8.1 7.7 7.6 7.6 83 80 3,0 7.8 8.1 73 7.7 7.1 7.9 6.7
Temp.('CI: 25.0 24.7 24.9 244 25.0 24.7 24.9 245 250 24.5 25.0 24.4 25.0 25.0
Sample Information sample'. Sample 2 Sample 3 Como) Analyses Survival Growth
Collection Wart date. 06.05-22 0647.22 06.09.12 !.wT.,- I Normal: Yes Yes
Grab: • . • Hons.Var. Yes Yes
Composite duration: 24•I, 24-I, 24-h _ t' . NOEC: 100% 100%
Alghnity(mg/I CaCO,l: 110 150 140 60-64 LOEC 3.100% v100%
Hardness Inr/t CaCO,): 24 24 26 85417 ChV: >100% >100%
ConduaNny(pmhos/tm): 447 515 478 296-305 Method: Visual insp. Dunnett's
Total residual chlorine)melt): <0.10 <0.10 <0.10 ,`p' S;;ia.j'
Sample Temp.at Receipt ITC): 18 3.3 3.9 ;:7= Arrival Growth
%Effluent Critical Cakulated Critical Cakutated
9.25% . 2.410 2180
185% 2A10 1.077
37% _ . 2.410 1.201
74% • 2.410 2.154
Overall Analysis: t00% . 2.410 1035
Result: PASS LOEC: >100% NOEC: 100% ChV: >100%
DWR Report Form AT-5
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EaNinnmmrNTerdn9 S,Ioslom.Inc.
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1000.0)
Species: Pimephales promelas
Client: Tryon WWTP County: Polk
NPDES#: NC 0021601 Outfall It: 001
Project#: t hAat¢
Dilution preparation:
Dilution prep(`°) 9.25 18.5 37 l 74 100 Sample was not aerated or treated unless otherwise
Effluent volume(ml) 92.5 185 370 740 1000 noted on this form.Sample was warmed to 25.0±1.0°C
Diluent volume(mL) 907.5 815 630 260 0 in a warm water bath and then diluted to the test
concentrations with moderately hard synthetic water
Total volume(mL) 1000 1000 1000 1000 1000 (MHSW).
Test organism information: Test information:
Organism source: In-house culture Randomizing template: ltbtte ep_Je_. i
Incubator number
Age: c 24-hours old and shelf location: b_
Spawn date: OS-NI,'tt Artemia CHM number: CHM1149
pt..Otn•tt %S00 r0 Drying information for weight determination:
Hatch dates and times: O(i•01•1.1._ CAS Date/lime in oven: 4l.•W1.1, OgOb
'Initial oven temperature: (nO•
pH(S.U.)= "t. Date/Time out of oven: r1,tl•tl 0&00
Transfer vessel information: Temperature(°C)= 151-0 `Final oven temperature: tor-'
Average transfer volume(mL): c 0.25 mL Totai drying time: 1A-N0J?-S
'60°C Oven,Thermometer SN:14-98595
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation,renewal, Sample MHSW
or termination number batch used
Time Analyst Time Analyst Time Analyst used
0 06-07-22 °A1SS yk tto0 IA_ O1'•I6 In "1.1ttAf..0t oVOt•'llL-
1 06-08-22 DSoU i` 11b0 tt 0941-
2 06-09.22 0S00 1t1 1‘00 K OISO a -ma,ok•0\ 0(,•0(o't1VI
3 06-10.22 OSA° VI, 1100 }f 0-60 1,1{ 14 06-11-22 0` f ok -06.111C-
o53D � 1it5 Ao8o+a � -,1�rn. nbI.5 06-12-22 OSoo I,( (r 00 1S0 K
6 06-13-22 OSoo 8\ 1{00 y( CI1S0 t Ob•Ort-1.1.
7 06-14-22 , C)1 Sb ti o`
Chemical analyses: `
Parameter Reporting Limit Method number Meter Serial number
pH 0.1 S.U. SM 4500-Hu B-2011 Accumet AR20 93312452
Dissolved Oxygen(0.0.) 1.0 mg/L SM 4500-0 G-2016 Y51 Model S2CE 180104324
Conductivity 14.9 pmhos/cm 5M 2510 B-2011 Accumet AR20 93312452
Alkalinity 5.0 mg CaCOr/L SM 2320 8-2011 Accumet AR20 93312452
Hardness 5.0 mg CaCOi/L 5M 2340 C-2011 Not applicable Not applicable
Chlorine,Total Residual 0.1 mg/L ORION 97-70-1977 Accumet AB250 92349123
Temperature 0.1°C SM255013-2010 Digital Thermometer 13nI44ttS
Control information: Acceptance criteria Summary of test endpoints:
'A Mortality: 0 7. 5.201/4 7-day LCsa(%) 7 I Do?,
Average weight per initial larvae: 0•6 VA NOEC(%) I DO 7,
Average weight per surviving larvae: 0•e 14 >_0.25mg/larvae LOEC(%) 1 100 7.
ChV(%) 'o 10p 7.
ICU(%) 71007,
SOP AT2O-Revision 6-Exhibit AT20.3
4 of 14 8/24/2022,7:32 AM

Page 2 of
E,nI,anmc,iai iraing Satticmt Inc
Species: Pimephales promelas
Client: Tryon WWTP Date: 06-07-22
Survival and Growth Data
Day CONTROL 9.25% 18,5%
ABCDE F G H I 1 K L
0 10 10 10 10 10 10 10 10 10 10 10 10
1
10 10 10 10 10 (0 10 10 10 /0 I0 10
2 t0 to /0 10 10 10 10 tO t0 10 /0 (0
3 JO /0 /0 10 10 ID 10 To /0 10 +0 / 0
4
10 IC) ID 10 10 10 10 ID (0 ID 10 Ip
5 10 I0 10 10 10 10 10 ID ID 10 (0 10
6
ID 10 ID 1t) 10 10 ID /0 ID 10 10 1D
7 It tp i0 ID l0 to 10 it) t0 lb it) 10
'A=Part weight(mg)
Tray color code: PI Li
Analyst TC (N.$N (Z 13-
(S 1�P1 ( 15 58
Date: o r'-t3-tt �'3(3,89 IS.76 14.50 I Q� ��•63 11..79 15.
=Pan it larvae weight(mg)
Analyst. T(r Vikate.75 LN.ly v.'7 V-. 1.137 LI.H3 14.' i 7.t.57 2o,20 Z -'S 22.116
Date: O b-19-1b
C=Larvae weight(mg)=B-A
8.14 l_-tg €. 1 $.04 10.g10 g,21 g.tl2 1.4g to.914 1,41 1.10 8•140
Analyst: Xl
Weight per initial number of larvae(mg)
=C/Initial number of larvae 0 Q
reY. 'Cr' � rbog �e ^ �S' nab \; A ' e
Analyst: t l 0• O' 0• 0• 0• 0' 0 0' 0- 0' 0' 0
Average weight per initial Percent
number of larvae(mg) reduction -€.1.4 0.1b$ t..g7, 0.116 S.S 7.
from
control(%)
`Weight measurements performed using Cahn 28 Automatic Electrobalance,SN 41520.
Comment codes: c=clear,d=dead,fg=fungus,k=killed,m=missing,sk=sick,sm=unusually small,
Ig=unusually large,d&r=decanted and returned,w=wounded.
Comments:
SOP AT2O-Revision 6-Exhibit AT20.3
5 of 14 8/24/2022,7:32 AM

Page 3 of 5
tmlromwnnlT.snng S.IWanc Inc
Species: Pimephales promelas
Client: Tryon WWTP Date: 06-07-22
Survival and Growth Data
Day 37% 74% 100%
MN 0 P Q R S T U V WIX
0 I
10 10 10 10 10 10 10 10 10 10 10 10
1
1D ID 10 10 ID 10 10 10 ID 10 t0 to
2 (0 10 10 10 IO 10 10 10 ID ( 0 10 10
3
(o lD 10 10 I0 10 ID 10 J- .c
a 10 10 /0
4 10 10 10 10 10 to (D to et t0 In Ip
S (0 ID to ID 10 10 ID ci ID to 10
6 ID ID ID ID ID (0 ►O 10 q (0 (0 Ip
7 I0 10 10 10 ID !0 10 )0 9 13 t0 tt
'A=Pan weight(mpi
Tray color code: I)/V(
Analyst:.Gf 13,115 15.16 I11.99 11150 is.8o 15.34 I5.63 14.79 14.(g 1Y2,9 19.49
Date: o S✓Z�-21
'B=Pan♦larvae weight(mg)
Analyst: TO' 7,1 9q v g1 LIAL1 L
Date. ob-11-1.'I, til• 9 L7..$3 t2.St 11,74 Zt91 Za,$'1 zz.80 7-1-1/ ZI.z3
C=Larvae weight(mg)=B-A
8.`I`I 1.6S 1.AS 6,'141 (..13 1.I 1.1I 8•i2 b.610 g,ob 1.39 0.7y
Analyst:
Weight per initial number of larvae(mg)
=C/Initial number of larvae 0"‘
7. n 'p % '9 n
analyst O O O O O 0- 0 b 0 ti O o'
Average weight per initial Percent
number of larvae(mg) reduction — 1 � 0.11q lk•b7- 0 -13� 11.0 i.
from control 0• 1 1 ,S ].
1%)
'Weight measurements performed using Cahn 28 Automatic Electrobalance,SN 41520.
Comment codes: c=clear,d=dead,fg=fungus,k=killed,m=missing,sk=sick,sm=unusually small,
Ig=unusually large,d&r=decanted and returned,w=wounded.
Comments:
SOP AT20-Revision 6-Exhibit AT20.3
6 of 14 8/24/2022,7:32 AM

-LI
J
0 CD
_ X
E•...•,m.d inlhpS.N....
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1000.0)
Species: Pimephales pramefas
Cr0.40 be.
Facility: Tryon WWTP toottoo,e,
rest start date: lune 07,2022 vnedbyProject number: 16998 ine wmn;r
Sample numbers 220606.01,220608.01,220610.01 IA
Con,..,r•,.•n lxlnone... I m ,.r Ilolwnn.rolbm.••Ono e,Mlmd 6.loan•sm.. Son...elgMlrnd W.-VA/1voi.or •. ne/ Mal.Aaroa W..16/,eI.I..Ise, Moan.un6.l n,/ 7,.fIoI.n,of v.rktarn Per. ..t nI ,..r
,.n .i.. ..Mlmd •e.• ,919n9111 l.0a.Imd s• Iw r x l .v-.• r n.-- e,l.n..s^`d IS) Mnnon..a
l non.. MI canoe,I*I
u,...lnll ar..«ds) I.0,e1^'0
A 10 SO 14.84 23.62 8.78 0.878 0.878
8 10 10 12.96 20.75 7.79 0.779 0.779
Control
C 10 10 I5.83 24.10 8.31 0,633 0824 5.1 0.833 100.0 0.624 5.1 Nat applicable
D 10 10 13.89 21.97 808 0.808 0.608
E 10 10 15.70 22.66 6.96 0.696 0.696
F 10 10 14.50 22.77 8.27 0.827 0.827
9.Z5%
G 10 30 13.42 21.43 8.02 0.802 Olbtl 76 0.802 300.0 0.76E 7.6 6.8
H 10 10 L1.91 19.39 748 0.748 0.748
I 10 10 14.63 21.57 6.94 0 694 - 0.694
38.5% 1 10 10 1279 20.70 7.91 0.791 0.791
0 776 6.9 100.0 0.776 8.9 5.6
lc10 10 19.15 22.75 7.60 0.760 0.760
l 10 10 13.88 224E 8.60 0.860 0.860
M 10 10 13.45 21.89 844 0.644 0.844
31X N 30 10 15.l6 22.81 7.65 0.765 0.765
O 10 10 14.99 22.94 7.95 0.795 0771 9.0 0.795 100.0 0.771 90 fig
P 10 10 14.50 21.29 6.74 0.679 0.679
O 10 10 15.80 22.53 6.73 0.673 0.673
R 10 10 15.34 22.52 7 18 0.718 0.718
70>. 6 10 10 15.61 22.74 7 11 0.711 0 719 B.l 100.0 U.729 8.1 11.E
0.711
T 10 10 14 79 _ 27.91 8.17 0 612 0.812
U 10 0 14.18 20.84 666 0.740 0.66E
✓ 10 10 14.74 22.80 8.0E 0.806 0.80E
100Y. W 10 10 14.29 22.18 7.89 0.769 0]57 ].9 97.5 0.134 10.1 1f.0
0.789
4 10 10 1449 21.23 674 _ 0674 0.674
0uda11001' A1S0, Minimum Significant Dille:enc.
Ounnett's MOO value: 0.1069 PMSD- Percent Minimum S:gn1icant Difference
PMSD. 13.0 PMSD Is a measure of tett precision the PMSD lsthe minimum percent difference between the control end treatment that can be declared statlsticelly significant In a whole effluent 106,6,1y test
lower PMSD bound determined by USEPA(10th percentile)=12%.
tipper PMSD bound determined by USEPA(90th percentile)•30%.
Lower and upper PMSD bounds were deter m,ned from the 10th end 90th percentile,respectively.of PMSD data from EPA'S WET Interlabarawry VanaAAily Study(USDA.2001e:USEPA,2001b).
USEPA,20014.2001b,final Report:Inteil.boratory Variability Study of EPA Sh4n-rerm Chronic and Acute Whole Effluent TociclryTesl Methods,VoNmes 1 and 2'Appendla.EPA-821-9'01-004 and EPA-821-6-01'005.US Environmental Protection Agency,Cincinnati,OH.
00
N
A
tJ
C. P
N Cr
N 0
0
J , -
W Cr
N.) 23
a x-
K

AA
Statistical Analyses
EA.nw,k.ra n,we l«Aihm,..
Larval Fish Growth and Survival Test-7 Day Growth
Stan Dale. 6/7/2022 Test ID: PpFRCR Sample10: Tryon
End Dale: 6/14/2022 Lab ID. ETS-Envir.Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol FNCHR-EPA-821-R-02.013 Test Species: PP-Pirnephales promelas
Comments:
Cent-lb 1 2 3 4
0-Conlrot 0.8760 0.7790 0.8310 0.8080
925 0.6960 0.8270 0.7980 0.7480
18.5 0.6940 0.7910 0.7600 0.8600
37 0 6440 0.7650 0.7950 0-6780
74 0.6730 0.7180 0.7110 0.6120
100 0.6660 0.8060 0.7890 0.6740
Transform:Untransformed 1-Tailed Isotonic
Conc-% Moan N-Mean Mean Min Max CV% N t-Stat Critical MSD Moan N-Moan
0-Conbol 0.8240 1.0300 0.8240 0.7790 0.8780 5.075 4 0.8240 1.0000
9.25 0.7673 0-9311 0.7673 0,6960 0.8270 7.511 4 1.280 2410 0.1069 0.7718 0.9366
18.5 0,7763 0.9421 0.7763 0.6940 0.8600 8.882 4 1,077 2.410 0.1069 0.7718 0.9366
37 0.7708 0.9354 0.7708 0.6790 0.6440 8.990 4 1.201 2.410 0.1069 0.7708 0.9354
74 0.7285 0.8841 0.7285 0.6730 0.8120 8.109 4 2.154 2.410 0.1069 0.7311 0.8873
100 0.7336 0.8905 0.7338 0.6860 0.8050 10.087 4 2.035 2.410 0.1069 0.7311 0.8673
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk s Test indicates normal distribution(p>0.01) 0.94367 0.684 0.00751 -1.1936
Banlelts Test indicates equal variances(p=0.96) 1.00249 15.0863
Hypothesis Test(1-tail,0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df
Dunnelt's Test 100 >100 1 0.10686 012968 0.00475 0.00393 0.3452 5.18
Treatments vs 0-Control
Linear Interpolation(200 Resamples)
Point X SD 95%CL(Exp) Skew
1C05• 7.294
IC10 64.219
IC15 >100 I 0
IC20 >100 0.9
Ic25 >100
IC40 >100 0.8-
IC50 >100 0.7-
•Indicates IC estimate less than the lowest concentration 0-6-
•
a0.5•
0.4-
0.3-
0.2•
0.1•
D.0 •
0 60 100 r50
Dose%
Dose-Response Plot
0.9
0.8 S o 1-tail.0.05 revel
0.7� _ of significance
oa
c9 0.8
a+
p 0.4
03
02
0.1
01 •
} o c
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Page 4 of 5
Environmental Tn nng Solutions.Inc
Species: Pimephales promelas Date: 06-07-22
Client: Tryon WWTP
Daily Chemistry.
Temperatures performed at the time of test initiation,renewal or termination by the analyst identified in the Daily Renewal Information table
located on Page 1.Alkalinity,hardness and chlorine(total residual)performed by the analyst identified on the bench sheet specific for each
analysis and transcribed to this bench sheet.
Day
(Analyst identified for each day,performed pH,0.0.and conductivity measurements only.)
n(+2-tt 0 1 2
Analyst .A ),.L A -l�
Concentration Parameter /��s V`� +� V� �}�-(} /� "7
pH(SU.) "+,1�_ 'n.`(/'10 .' 1 J.5 J k1 ! I Cs t,�
Dissolved oxygen(mg/LI 1'Y 14.'1 7) '4Pj —}',e 7<4
CONTROL, conductivity(pmhos/cm) ZQg , Cl(p 351
MHSW Alkalinity(mg Caco.s) 64 \. 4,.4)t b3
Hardness(erg CaCO,/l) 11 l�� fi 1
Temperature(°C) .4.. 10.10 /-LA•k - 1`s•"1 �-7Ut.to
pH(5.H.) ,PAP q.lef0 Y LQ� • 1 J•i'cD
9.25% Dissolved oxygen(Mr./1)1) D //.s., /3_1_ N ,Cf
Conductivity(umhas/cm) Qr)7 I-- MI111111111111M111111
Temperature(`CI "L'n A Z:t.lp ^t1A A �'-'1.�1r
pH(S.U.) '4•.L7V 1,}2 C.TI /.(
Dissolved oxygen(mg/LI ie...0 4 co /),k4 d 9 CS.f7
19.5% 3 32q 321
Conductivity(pmhos/cm) ,f ��
Temperature(°C) /''t.4.\ I •-L4-'1 Zk-' 1,771.4A
pH(S.U.) -1-A i �',1'^f 1-. 6 i i
Dissolved oxygen lmg/L! (t,p �•Y S is Ci
37%
Conductivity(pmhostcml 3(p 1 U9 IIIIIIIIIIIIEUIIII
Temperature(°C) �115.0 i- -1 -1.,�.' 1 •Ly. & -LA.' V41-(o
pH(5.U.) 640 i, °)e, 'IA 2_. MillMIES11 -7,U!
O,,solved oxygen(mp/L) (3.0 4 t, �'J}.J % 6•Q
7490 Conductivity(pm 4
hos/cml 09 3qv i1
Temperature('C( ZSS�.0 /�1-4.. rZSyp_/ . Z4(/ice
pH(S.U.) 0.06 llf94 'fS I'"1 J , ma '7.-I
Dissolved oxygen(mg/LI .0,1 J-,1 �-It 1 . : _�'''0
Conductivity(pmhos/cm) LiLjl. Li 31— 5)s
100% Alkalinity(ergCaCO3/L) I1O IS
Hardness(mg CaCOrh) -t:4 -1..4
Chlorine Img/U 0,10 4-0•I D
Temperature(°C) LS.0 'L .1 "IA-''i I Z`t-(o tc.o ZI•-I
Initial I Final Initial Final Initial II Final
SOP AT2O-Revision 6—Exhibit AT20.3
9 of 14 8/24/2022,7:32 AM

Page 5 of 5
- I,e,,m fl1.F,,,n 9s3Iwn�,let
Species: Pimephales promelas
Client: Tryon WWTP Date: 06-07-22
Day
(Analyst identified for each day,performed pH,D.0-and conductivity measurements only.)
3 4 A 5 6
Analyst TAi NSCi _ PF,e- pFjL. C- II IA-
- V v IA--
Concentration Parameter
pH(S)u.) 7I(,i, 1.16 1.1e+ 1.5t. •-to.Z 'l:s3 ' G'i- 'r.te
Dissolved exy(mlrrre/U -rt7 • .0 1 - ! 'I-4 7-i- '4.0 "i.to ' j
CONTROL, condeni.nytiermeshml /).,0)%1 .304 sop 303
MHSW Alkalinity(m(GCO,IL) G`"LY „ 11 �` la0
Hardness lm(GCOJL)
` ef S '\ &S
Temperature rn -LA."l tV r`t " -S ti1.-1 -n .1 "LA-L "l.'k-8 I 1A-1
nH(5.u) -7i7 1.16 11el j1.St 4.11 '}51. is ' 2.Z
olssolvedoayeeo Pock) --1,1 .0 -4.1 -1.4 14- '4.7- .m to.t
9.25% n 7
Conductivity(pronto/ant -)L1 ) 319 ;35 3 D
Temperaturera 1-'4•1 -1.A.6 --`l-4 Ln1.lo z.`l.fr 1A.6 1.A.& I IA.is
pH MU.) -)y7% 1q0 �.3q l 1,61 1.�� 'c �-,18 '4,2-
Dio,oirede.y(enlm(nl �1/.`I -4 Pt 8.3 1,3 3 1 ` -,1 1.6 (0.�
18.5:° Conducivitylumhos/cm) [)Ca 33 F 31S 0,4?,
TemperaturerC) 1.,1-fr-LA-le -ia-5 -1.A.1 -IA.1 '1,1,-1 vt.1 �1r&
pH15u) (:ii`I 7.10 „elO i 1.� ac_7.71k f 14.50 1- ' '1,40
Dissolved eerten(m(/L1 c`)1 1,7 1..0 13 4,1- "}rl q t.0.--
37% Conducprity(pashas/cm) ...,:),--r- 31-3 _3s+ 315 I
Temperature rd A.)-1-& 1 f&•5 -ts•0 1A.1 Z`l.`t -)..4.b .'.rl-'\ 'LA`t
pm(s.u.) •i,' 7 qv 'i-Dd. Z.Qo 1.q4 ' .'•t 3 �e
Dissolved oxygenIml&1 ��..,�.0.I 4 �•� 33 +114 '4,1 ,ice}" I •Z
74% Conductivity(pmhos/cml / /s 1+30 LKA -t s39
TemperatrerOO Z`l-& tr1•s '\.5.0 I \A.2 As.0 1A{-1 14.1 IAA-5
pH ts.u.) c{y,i: _ :.qci 8.)a. j -. 4 T.IO '1r4'1 /at0 1-fat
Dissorvedr.,vn(meN co 5f) g•$ c.l j 1-�- ' rI '�q (0.1-
nvll Condu � r Wmhos/cml Gj jr=) '4-1 4_99_ 72,
100% Alkalinity One GCO,/0 _ i'4o I _ r
Hardness(mg GCOJL) 14,
Chlorine irrI/L) 4 0.10 , ,
Temperawrerq -IA.`l 1-A•S 1S.o 1`1-s 15.0 1_4.4 tS.O 'LC.0
Initial Final Initial 1 Final Initial L Final Initial j Final
SOP ATZO-Revision 6-Exhibit AT20.3
10 of 14 8/24/2022,7:32 AM

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} 351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Tryon W WTP NPDES N:NC0021601 Pipe N:001 County: Polk
Purchase order:
Species Pintephales prontelas Effluent dilution: 9.25,18.5,37,74,100%
Test type. Full Range Chronic Parameter code: THP6C
Sample information: (to be completed by sample collector) nn
Composite sample: Pie_�l Sample location:s@j ei�h �B ol�4A CIP011II
Start date: G,�S— 22 Time: Volume collected forr fa ling: a..op
End date. 6-G—1Z Time: O /) Number of containers filled for testing! 2
Number of samples per(tour l. Method of transport to laboratory: vd9J/
Chilled during collection? L,fpf Comments:
If chilled,specify temperature: u 2
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice.The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Semple collected by:
� / (PI(PI az.
MtC.kr I F,4 ovoo
nm Sfmuae Dryad Knee
Relinquished by: Received by:
nw sg,mwDow me taw
na.wes
Relinquished by: Received by:
n.. spane< n,ww.,r Data sin.. wk a.ays
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
62-/6 4732- 06 66-1Z
�dt:l�� ii �h CxEF �4� � o.. SSIN S
Custody seals intact?: n n Sample temperature upon receipt at ETS(°C): I
g,C
Ye Nw m.tuJN
Samples received in good condition?:1
Y� x. Total residual chlorine upon receipt at ETS: d
i� (DPD Presence/Abu-sue Indicator.MDL-0.10 mg/LI iRitlM '1PY
Tracking number: j(i"
Project number:16 Q4°Sample number:22°I`ao.D
Comments:
11 of 14 8/24/2022,7:32 AM

351 Depot Street
Asheville.NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions.Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility Tryon WWTP NPDES is NC0021601 Pipe 001 County Polk
Purchase order
•
Species: Pimepltales prome/as Effluent dilution 9.25,18.5,37,74,100%
Test type. Full Range Chronic Parameter code: THP6C
Sample information: (to be completed by sample collector) q
Composite sample: Sample location: + /�v�l� iS1it7/10,:Y/Tr
Start date: (p 1 71 1Z Time: 0`i'60 Volume collected or testing: .L
End date: L./RI .L Time: �-
I' ��JU Number ofcontaincrs filled fortesung:
Number of samples per hour: Method of transport to laboratory: 1 ry nrf
Chilled during collection? 44' / Comments
if chilled,specify temperature: 2 O •
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
9Y SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample collected Sr:
II A^ /a-0-7-2
rCt�i4rQ Free ke�i</ V—e le)fGc
Relinquished by: o w (Smelted by
Relinquished by: Received by:
•
sgmia, onr.a r.
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received of ETS by:
CA Oct
Custody teals intact?: EJ © Sample temperature upon receipt at ETS(°C): . L
Samples received in good condition?: r
° Total residual chlorine upon receipt at EIS: 1
(DPD Presence/Abccnlc Indicator,MOL=0 IOmglL)
Tracking number: ►t n r{ r LOU--
Project numher:117q 9e Sample numbe tVJ 1 t
Comments: c9 0(00601
12 of 14 8/24/2022,7:32 AM

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351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility. Tryon WWTP NPDES if: NC0021601 Pipe II: 001 County: Polk
Purchase order.
Species: Pinrep/rales promefas Effluent dilution. 9.25,18.5,37,74,IOW A,
Test type Full Range Chronic Parameter code- THP6C
Sample information: (to be completed by sample collector) L
Composite sample: /,, Sample location:. ,/,e.42 k. CLOiCed
Start date: /q/2 Z Time: (J Ae. Volume collected for testing: 3 pc,A.L(pr,/
End date: to Ib/>2 Time: Q(•64) Number of containers filled for testing: 2
Number of samples per hour 4 Method of transport to laboratory: J rypf
Chilled during collection? r 0 Comments:
If chilled,specify temperature: 2
Triple rinse sample container with sample before filling.Completely till the sample container with no air space.
Pack the sample container completely in ice.The sample must be<6.OeC upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Semple collected by:
I /0-t0-jZ
Mi*Mod cPorea/4- D •Oo '
Nei Sp.* law antiv
Relinquished by: Received by:
M,I t,& c-446(r.t/ ,61. - bass-
Doe and one 11m swot.. ameaw�e
Relinquished by: Received by:
r ' ?
Pt.. Rpw, Ode a1.4 A-. s,r...e taaw�
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
08
• ti LA-
Did .s
14w S,psNe aai We: n4 srpeoe Dak as
Custody seals intact?: [J [J n Sample temperature upon receipt at ETS(eC): 1 l
0. No Maumee �y IA, `
Samples received in good condition?:
n N^ Total residual chlorine upon receipt at ETS: r,
••,, (DPD Presence/Absense Indicator,MDL-010 mg1L) trosx 'v""
Tracking number: r� 'I_/?(� /gyp`/t Project number: 141 Sample number:01 (01`t.0
Comments:
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Pimephales promelas
Chronic Reference Toxicant Control Chart
Enm.anmantai Tasting Solution s,inc. Source: in-house Culture
0.9
Standard Deviations)
Limits(± 2
0.8 - -
• •
•
0.7 - • • • •
• • •
• • • •
U •
v •
0.6 - -
t
Ur4 1.2 I I I I I I I I II i
ca - Warning Limits
n
1.0 -
• • •
• S• • • • •—• •
— — •
0.4 -
I I I I I I 1 I I I I I 1 t l I I t I
p31p 0a.1O 0511 01;6'1 p.L;IS 0y 7.1 pp 1'1 pg 1'1 �31.1 p3;L1 gyp 11 OS:Ll p1.11 0�.1Z 0,11 001-O.11 p.1�'.0.y 1Z 0i.11
OS" O6. p1" p8. 09. 10. 1.v 11" p1. 0 03. pa OS p6.
Test date
• 7-day ICzs=25%inhibition concentration. An estimation of the potassium chloride concentration
which would cause a 25%reduction in Pimepholes growth(calculated using ToxCalc).
Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
Laboratory Warning Limits(mean logarithmic IC.S±2 coefficent of variations converted to anti-logarithmic values)
- USEPA Warning Limits (mean logarithmic IC,±SA„converted to anti-logarithmic values,
SA,S=75th percentile of CVs reported nationally by USEPA)
14 of 14 8/24/2022,7:32 AM

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PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
June 20,2022
Ms.Deborah Bradley
Town of Tryon
423 North Trade Street
Tryon,NC 28782
ETS Project Number: 17012
Test Start Date: June 08,2022
Facility Tested: Tryon WWTP
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Parameter Result
Code Test Procedure,Method Number PASS FAIL
TGP3B North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure v .
EPA-821-R-02-013,Method 1002.0 /�
All toxicity testing results required as part of your permit must be entered on the Effluent Discharge
Monitoring Form(MR-1)for the month in which it was performed,using the parameter code TGP3B.
Additionally,the DMR Reporting Form AT-1 must be signed and e-mailed or mailed to the following
address:
ATForms.ATB(a�ncdenr.gov
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh,NC 27699-1621
Please call if you have any questions concerning these results.
Sincerely,
CtfnSumner
Laboratory Supervisor
North Carolina Certificate Numbers: Biological Analyses:037,Drinking Water. 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act:99053-001
2 of 10 7/7/2022,8:34 AM

PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
Effluent Toxicity Report Form-Chronic Pass/Fail and Acute LCsa Date: June 20,2022
.-/Ki
Facility: Town of Tryon NPDES fi: NC 0021601 Pipe it: 001 County: Polk
Tryon WWTP --; ,l/ -2
Laboratory Performing Test: Environmental Testin Solutions,Inc.,Certificate if 037 Comments
Signature of Operator in Responss:ble Charge(ORC): Fa, lav,j../ a ed� I
xli ORC Phone/E-mail:cle vl4it[i (2] r. e,,rn� Project u: 17012
Signature of Laboratory Supervisor: c l „ZAr- - Sample S: 220607.14,220609.02
e-Mail to: ATForms.ATB(aInc enr.gov
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-stat/Rank Sum -4.32
Raleigh,NC 27699-1621 1-Tailed Critical 2.508
%Reduction: -15.5
Percent Average
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Mortality Reproduction
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Control Control
Number of Young Produced 30 28 32 32 30 29 34 29 30 33 31 29 0.0 30.6
Adult Survival. (L)ive,(D)ead L L L L L L L L L L L L Treatment 2 Treatment 2
0.0 35.3
Effluent Percentage 37% Control CV
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 6.0 PASS FAIL
Number of Young Produced 31 37 41 38 35 32 33 39 36 31 38 33 0r ,,, r X
Adult Survival: (L)ive,(D)ead L L L L L L I L L L L L 100.0
pH(S.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date: June 08,2022
Control 7.59 7.75 7.68 7.78 7.57 7.70
Treatment 2 7.87 7.91 7.83 7.97 7.86 7.95 Collection(Start)Date-
r 2 t - Sample 1 06-06-22 Sample 2 06-08-22
D.O.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control 7.7 7.7 7.7 7.7 7.6 7.8 Grab Comp. Duration
Treatment 2 7.8 8.0 8.1 8.0 7.8 7.9 sample 1 • X 2a-n
c w w
Sample 2 . X 2a-, ? 3 E
o v, ,n
LC50/Acute Toxicity Test Alkalinity(mg CaCO,/LI es.64.60
(Mortality expressed as%,combining replicates.) Hardness(mg Caco,/L) 87,87.85
Conductivity(pmhos/cm) 296,305,3133 500 487
Concentration(%) Total Residual Chlorine lmg/L) <0.10 <0.10
Mortality(%) Sample Temp.at Receipt I°C) 1.9 3.2
LC50= Method of Determination
95%Confidence Limits Trimmed Spearman Karber t i, r
to Probit w w
Other: Control
High Conc
Organism Tested: Duration: pH(S.U.) DO(mg/L)
DWR Report Form AT-1
3 of 10 7/7/2022,8:34 AM

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__ _ Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species:Cerlodaphnla dubia
(EPA.821.R-02-013 Method 1002.0,NC Modification—December 2010,Version 3.0)-Control Bench Sheet
Control 0:1 Date: 06-08-22
Test Grouping Information: Test Organism information:
Facility Project A Organism Source: In-borne Culture
6 -nazi o rJ tr 01L Age: <24-hours old
5 'PI A)b cm.e. 1100t1 Source iculture board): OS•31-1t A OS• 1'12.$
4 (•t.IFGSI�fl. 11 D0t' RepllcateIf 1 2 3 4 5 6 j
0 9 10 11 12
3 'lifLe45hl1..e. t1011 Culture board cup a lA yp 311'../iL 3) 2 8 ls st 11-
2 P SAeJllLe AS11 I l o i6 Date and rime organisms were born Ot•0t•11 O`l0S 0400
1-Control between:
A transfer volume: <0.25 mt
Transfer bowl Information: pH IS.U.I: 1.i0
Daily Renewal Information: Tampentwel'Cl: 1S-O
rnrlNaradon,rmeraa,-twain,w ,plennal.rnn ywr loot* 9aMamlaln/
Ow Date ttrmMallan WOW Bald
Time Analyst Bald batch I..iota/RNe ...plate
0 D6-0Ba2 w lgaS" 0i•o1•tLC oi-o- It Otr-UL•11 1..L Oit.o,p3Ce
1 06-09-22 Tero A.i
2 06-10-22 Ae11"1Aaenj
t�4la1O C OLo4118
3 06-11.22 '/f A`.f
4 06-12-22 ;740 e/'6
5 06-13-22 a"n'"inw ,1
CIrjS� V1 0b0411'6
6 06-14-22 7/70 h.(tl
7 06-15-22 Th•L1
•Creamamt rd dui,SOot MC telemmwum sod SO yr err mitiotte wins Nandylree ream abNbr SR I7fS91Sa.
Chemical Analyses: IMHallon Renewal One Renewal Two
Initial �F^ina.l Initial Final Initial Final
Concentration Analyst � 1 V V 5\h-, z)/s/ 11A-)
pH(S.U.1 1,s9 —7I1-S -7r OS 7r 1.51 '7.70
Dissolved oxygen(mgAl '1_'1 "111.'-) —77 '1.6 '7 k
Condurttvltylemllas/eon) 1.e1b ! ( ,)<-1 3a'3
Comas.MHSW 'Alkalinity imen.CaCOJ LA l03 fah
'Hardness breftCaC01) & 1 Al ks
eTemperaturaltl 1.1.t¢ L-fixes 1s1.1 - 1. 1.4.-1 1_5.\
•A.o o Ar:fwd for each d2.p,6...d pH,dnrdwdoaym aNm.dmo•t,meoarann0 only.Impostor.performed at de env olio,nitiatio..rntnd or Lenaiwiwrb,,lh:
auhat rdamifud O du Oak Ratnai Information ta6k Aaalktn and!wdnest performed b.the=oh.,kdauilod on de ter bawl,itau axe.rwambo to the bench am.
Iammaer Martina WWI Mashed naber MOe, Serial number
pN 91 S.Y. SM 450041.0.2011 Ktumt,AR20 91312a52
Olaol.ed oxygen 1.0nrtA SM4500-OG201G Kt 1101943M
Condunbry 14.9 imitation 104 2310 8.1011 ACOrmet 0020 9331102
alkalinity S.O mu CaCO,/t SM11201-2011 Aowant A9lo 933114S2
Na.dnev 5.0 al CaCOV1 SM 200 C-1011 Not appWable Not 4000ca e
t.uln.N..l w.n.. Q1s4A 0910r192-10-1927 Knowr Aa250 921a9.1123
a.atu,rene 0.1'C SM 75S00-2010 Oiplal Thr,reomnn, 1jae«6 tS
Survival and Reproduction Data:
Day Observations Replicate number
• 1 a 3 4 5 6 7 0 9 10 11 22
2 Adult mortality L L L L L I— L L L r_ L L
....don. It.on.0.dead)
Number of broods present L- L L L. L L L L L L
5 Number of Young produced 1_1 1 11 1i1 IS 1A I, 11a 16 to is I
Renewal Two Adult monanty L— L L L Lk_ L L L L L
ll.Live.11=dead' L
Number of broods present IL 1 t Li L L L_ L L t 1
Number of young produced t• Is lS 1� IS IS 11 13 t t 11_ Is
7 Total youngproduced 3b 1g 3•L 3t 3b 2y 3,1 2S 30 33 31 Zi
rival
Final adult mortality 1._ L 1_ L-- L 1_ L L L L. L k—
it.u.e.0.dndl `, �/
S for 3rd Broods t X x )L x X. )( /X V
x X. '
Control Acceptance Criteria:
r
X of Male Adults(5 20%) 7 Mean Offspring/Female 30.(o
It Adults having 3rd Broods fa BOX) fOot (215 offspring/surviving female)
X Mortality(520%) 01- %CV6,40%l 6-o1
SOP AT12-nedtbn 4-1da10k AT12.3
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Pagelofl
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0,NC Modification—December 2010,Version 3.0)-Test Bench Sheet
Paired with Control# I Date: 0 1.OL-17.
Client Tryon WWTP NPDES# NC 0021601
Facility . Outfall 001
Project# 11011— County Polk
Test Concentration(Chronic limit) 37% Dilution ml mL Total volume
preparation: Sample Dilution water mL
Samples were not aerated or treated unless otherwise noted on this form Control,dilution water Ill 189 300
and test renewal information are included on the Control Bench Sheet indicated above.
Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst Si j 3Vv `J W 71/ aW 7`n7
pH(S.U) 71‘es7 ion i. 3 -j, cr -7 6 -7,q5
Test Dissolved oxygen(mg/L) —7.s K-p c. -6-.0 c7 �r 7
Concentration Conductivity(µmhos/cm) »/Q 1(.; O7
'Temperature(%) 't,S-t •.s.0 IA-<P ZS• N. Z-`\-`1 ZS- 1—
pH(S.U.) C,1 Tj,0 b
Dissolved oxygen(mg/L) /(,O't ,6..I
100% Conductivity(pmhos/cm) -jJ" t-/Cj-7
'Total residual chlorine Img/L) i 0,10 40,10
Sample number Samp1e l "L1otal.iL•( tamale 2 ' 101p 0 di.0-1--
'Analyst identified for cock day,performed phi.dissolved o ygen and conductivity measurements only Temperatures performed at the nine of test initiation renewal or termination by
the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet Total residual chlorine performed by the analyze identified on the Total Residual
Chlorine Bench Shen and transcribed to this bend,sleet.
Survival and Reproduction Data (performed at test concentration):
Day Observations Replicate number
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality L L — L__ L 1_ L L
Renewal One (L=Live,0=dead) `---
Number of broods present L [ L , [ L t= L- LE t=
5 Number of young produced
tS eel -Le, tS tie t5 S1- IA X-1 I\n IO 1'1
Renewal Two Adult mortality l_ L L. L L L L L L L_
IL=live,D=dead) ,
Number of broods present y� L L L1 L L L. LL L=. Lt l�
Number of young produced Ito l f 6 2 I C1 I i l le l& t 15 l t
7 Total young produced . Y- Ai 3s S$ �1 SS SCt 3L 3‘ 13% 3
Final `
Final adult mortality L. L L L, L L L L L
IL°Live.D c dead)
Test was Initiated using Sample 1.Sample 2 was used for Renewals One(day 2)and Two(day 5).Samples were diluted to the test concentration prior to use with moderately hard
synthetic water and warmed to 25.0 21.0°C In a warm water bath.
Comments:
Test Results and Statistical Analyses:
Test results Statistics
%Mortality 0/. t-Stat or
Rank Sum _t1.31.0
Mean offspring 1-Tailed
2-So
per female `�5.� Critical sC
%Reduction -15.c 1. PASS or FAIL vt1SS
from control
SOP AT12-Re.tswn 1-fohiurt AT12.2
5 of 10 7/7/2022,8:34 AM

o,
21
0
24
0
- r ,,..mot.
rnvuoronontolTatinr Solution%Inc
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1002.0)
Species: Ceriodaphnia dubia
en,r,t,14y:
Facility: Tryon WWTP n„oouwd„., I
Test start date: June 08,2022 Minot
Project number: 17012
Sample numbers: 220607.14,220609.02
Concentration
Number of young produced by replicate number survival Average coefficient of Percent reduction from
(%) Day (%) reproduction(offspring/emale) variation(%) control(%)
1 2 3 4 S 6 7 8 9 10 11 12
5 17 13 17 18 15 14 17 16 16 15 19 14
Control 7 13 15 15 14 15 15 17 13 14 18 12 15 100 30.6 6.0 Not applicable
Total 30 28 32 32 30 29 34 29 30 33 31 29
5 15 19 20 19 18 15 17 21 17 16 19 19 +
37% 7 16 18 21 19 17 17 16 18 19 15 19 14 100 35.3 9.5 -15.5
Total 31 37 41 38 35 32 33 39 36 31 38 33
Dunnett's MSD value: 2.758 MS0= Minimum Significant Difference
PMSD: 9.0 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision.The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10n'percentile)=13%.
Upper PMSD bound determined by USEPA(90°'percentile)=47%. i
Lower and upper PMSD bounds were determined from the 10"and 901"percentile,respectively,of PMSD data from EPA's WET Interlaboratory
Variability Study(USEPA,2001a;USEPA,2001b).The lower PMSD bound represents a practical limit to the sensitivity of the test method and is
not a minimum acceptance criteria.
USEPA. 2001a,20016.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA•821-B-01-004 and EPA-821-8-01.005.US
Environmental Protection Agency,Cincinnati.OH.
J
J
N
Or.eilo
tJ O
00 E..
W Q'
A
a
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Entered hy:
Kell
�Pcv� .
Keenan
Statistical Analyses Sumner
U.,,,nmen,41Tesc,,ar,r
Ceriodaphnia Survival and Reproduction Test-Reproduction
Start Date: 6/8/2022 Test ID'. CdPFCRNC Sample ID: Tryon
End Date: 6/15/2022 Lab ID ETS-Envir.Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
Comments:
Conc-% 1 2 3 4 5 6 7 8 9 10
D-Control 30.000 28.000 32.000 32.000 30.000 29.000 34.000 29.000 30.000 33 000
D-Control 31 000 29.000
37 31.000 37.000 41.000 38.000 35.000 32.000 33.000 39.000 36.000 31.000
37 38.000 33.000
Transform:Untransformed 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
0-Control 30.583 1.0000 30.583 28.000 34.000 5.990 12
37 35.333 1.1553 35.333 31.000 41.000 9.451 12 -4.320 2.508 2.758
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution(p v 0.01y 0.97571 0.884 0 20477 -0.5602
F-Test indicates equal variances(p=0.06) 3.3228 5.31963
Hypothesis Test(1-tail,0.01) MSDu MSDp MSB MSE F-Prob df
Homoscedastic t Test indicates no significant differences 2 75797 0 09018 135.375 7.25379 2.8E-04 1,22
Treatments vs D-Control
Dose-Response Plot •
45
40
35
30 1-tail,0.01 level
;3'.
----•---- of significance
u 25
° 20
o.
a,
re 15
10
5
0
U
0
7 of 10 7/7/2022,8:34 AM

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351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
EnvironmentalTesting Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility Tryon WWTP NPDES 0: NC0021601 Pipe II:001 County: Polk
Purchase order,
Species: Ceriadaphniadubia Eflluentdilution: 37%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
�����,yyyyyy"""�7fy
Composite sample: Sample location: „RA/L/j'L..t 1
Start date: (O/'l 11_ Time: OU Volume collected foLing: EI
End date: Clo y 12, Time: (� OO Number of containers filled for tesfrfln : /
1 Number of samples per hour: T Method of transport to laboratory: Ode
Chilled during collection? r/ n Comments:
If chilled,specify temperature: � b
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice.The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample collected by:
��� � � I � • v/9 dtftiitit/e, go
iZ
Retingouhed by: Received by:
t/9/zZ 6 f
M,i�.,;e1 F ft_ ;a��fl 1•_ f1 Grip I o,(
uam,°euvr swv mr=m°a
rrenngn.nhed by: Received by:
mum IMIMMIRIRRasm
e Wu ands°r
Sample receipt information: (to be completed by ETS personnel) �6
Rdinqubbed to ETS by: Received e.ETS by:
61e.176.4e.1\ 4 ePa a,�e �!4 JL� O�
10)s.
Mai
Custody seals intact?: n n V.
y Sample temperature upon receipt at ETS(°C):
3.2
Samples received in good condition?: n
Total residual chlorine upon receipt at ETS:
(DPD Preseoce/Absense Indicator,MDL=0 10 mr/L)
Tracking number:
Project number;' )IL Sample number: ?jfi0i00g,07..
Comments:
9 of 10 7/7/2022,8:34 AM

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• li1.11111 Iwo our Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
EnvllonTlntal Testtno Solutions,Inc Source: In-house Culture
1.14 i I 1 I I I i r I I 1
1.12 - Control Limits (±2 Standard Deviations) -
1.10 - • • • • -
•
•
1.08 - • •
• ------ _
1.06 - -
ro 1.04 -
Z
1.02 - -
1 1 1 1 1 1 1 1 1 1 I 1 I 1 1 1 1 1 1 1
U,
r� - 1.....1.....1....1.....p....1.....I -
u
a 1.15 -
Warning Limits
1 -
•
1.05 -
1.00 - -
0.951 1 I I I 1 I 1 I ! 1 I I 1 I I I 1 1 I 1
01.11 O.t.11 O.L.11 Of'11.Op 11 OQ.11 1a 11 p311 la 11 10.11 05.11 01.11 0�-11 0�.11„,:0-0.11 001-05.11 03.11 0
01' 01' 03' 00' 06 06- 0T' 08' 06' p9' 10' 1.v 11' 01' 01 01' 03- 04 0S- 06'
Test date
• 7-day IC=s=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodophnia reproduction(calculated using ToxCalc).
Central Tendency(mean logarithmic IC2,converted to anti-logarithmic values)
—••--- Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
—••-- Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
USEPA Warning Limits (mean logarithmic IC25±Sa 20 converted to anti-logarithmic values,
SA is=lo'"percentile of CVs reported nationally by USEPA) ('
10 of 10 7/7/2022,8:34 AM

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PO Box 7565
Asheville,NC 28802
• Phone: [phone]
EmlrOnlnenlal lea,/SOW...Inc
Fax: [phone]
December 23,2022
Ms. Deborah Bradley
Town of Tryon
423 North Trade Street
Tryon,NC 28782
ETS Project Number: 17503
Test Start Date: December 14,2022
Facility Tested: Tryon WWTP
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Parameter Result
Test Procedure,Method Number Code PASS FAIL
North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure v
TGP3B EPA-821-R-02-013,Method 1002.0 /�
All toxicity testing results required as part of your permit must be entered on the Effluent Discharge
Monitoring Form(MR-1)for the month in which it was performed,using the parameter code TGP3B.
Additionally,the DMR Reporting Form AT-1 must be signed and e-mailed or mailed to the following
address:
ATForms.AT6(a�ncdenr qov
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
Please call if you have any questions concerning these results.
Sincerely,
rAN��
Jim Su ner
Laboratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
2of10 12/28/2022, 10:26 AM

PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
EnWr°rv++<Mal i<s+Irrg 5°IUUaus,ic.
Effluent Toxicity Report Form-Chronic Pass/Fail and Acute LC50
Date: December 23,2022
Facility: Town of Tryon
NPDES#: NC 0021601 Pipe#: 001 County: Polk
Tryon WWTP
Laboratory Performing Test: Environmental Testini Solutions,Inc.,Certificate#037 Comments.
Signature of Operator in Responsible Charge(ORC): -; {„f ' 5c;dhi`'
/�/ f CA/L1 ek Ad-MO
f J Project u: 17503
ORC Phone/E-mail: (1/ bn�y^ hr C't�f�' Ci%{'�-
Sample R: 221213.07,221215.01
Signature of Laboratory Supervisor: �i+!
e-Mail to: [e-mail]
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-Stat/Rank Sum -2.624
Raleigh,NC 27699.1621 1-Tailed Critical 2.508
%Reduction: -8.1
Percent Average
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Mortality Reproduction
Contra!
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Control C untr
Number of Young Produced 29 1 28 I 28 33 32 32 132 i 33 130 133( 31 130 0.0 30.9
fI1jI I If Treatment 2 Treatment 2
Adult Survival: (L)ive,(D)ead L L I L L L L I I L I. L
0.0 33.4
Control Cv
Effluent Percentage 137% .1 PASS FAIL
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 a<,<6.rm.or<d�<
Number of Young Produced 39 31 32 34 34 36 34 32 28 33 33 35 3aw°°a X
Adult Survival: {L)ive,(D)ead L L L L L I L L I L L L L L 100.0
pH(S.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date:
December 14,2022
Control 7.36 7.58 7.3117.541 17.34 7.42
Treatment 2 7.58 7.62 7.61 7.58 7.57 7.56 Collection(Start)Date:
4 J
Sample 1 12-12-22 Sample 2 12-14-22
a
w _
s
D.O.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control I 7.6 7.9 7.7 7.8 7.6 7.6 Grab Comp. Duration
Treatment 2 7.8 17.7 I 7.7 18.0 8.0 7.9 Sample 1 X Z,.h o
Sample 2 X 24-h ? a a
o 3 N
trl
Alkalinity(mg CaCO3/L) 61
LC50/Acute Toxicity Test
(Mortality expressed as%,combining replicates.) Hardness(mg CaCD,/L) 86
Conductivity(pmhos/cm) 310.308,313 352 370
Concentration(%)
Total Residual Chlorine(mg/L) <0.10 <0.10
Sample Temp.at Receipt 1°C)
1.3 1.0
Mortality(%) {II
LC50= Method of Determination
95%Confidence Limits _Trimmed Spearman Karber N N w
Prob
to Other
I
Control
Other:
High Conc.
Organism Tested:
Duration: pH(S.U.) DO(mg/L)
DWR Report Form AT-1
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North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodophnia dubia
(EPA-821-R-02.013 Method 1002.0,NC Modification-December 2010,Version 3.0)-Control Bench Sheet
Control 4: Z Date: 12-14-22
Test Grouping Information: Test Organism Information:
Facility Project It Organism Source: In-house Culture
6 ..-----%tt'h' Age: <24-hours old
nle5JlLl.e_ t"tt1�1C„ Source(culture board): ;t'Ob•'ll P5 it..1*-116
4 't r1s t"sC Replicate It 1 2 3 4 5 6 7�8 9 10 111 I2
3 N\,P VZJ %-t U Culture board cup 4-6 21 S1 31 yf1`(0I 'A 4 la III t
2 MAttSt1Au. OD-I... , 11SDC, Date and time organisms were born 1t'SA:t'L. 0600 T 0 1118
1-Control between:
Average transfer volume: c 0.25 mL
Transfer bowl Information: pH(S.U.I: 1_`-t"1
Daily Renewal Information: rempeearure l'cl: 'IAA
rest Initiation,renewal.Needing or S°lenastrvm ran location Aandamirine
Day Date termination MilSW Batch
Time Analyst latch Batch Incubator/Shell Template
Q 12-14-22 "°,S.L 1'`"` r` 11-,01-t t.d %\1 @ .t Ve '21 'L C.1 6%.NC1.•
►32
1 12-I5-22 re'd'°r
otea ‘,Sa ,
2 12.26-22 3enew.I1aree6•1
17A -1-- tt•Ot-11.8
3 12-17-22 naCI, AU
4 12.18.22 r O aCJO _ Als
5 12-19-22 -epabvtl hi 1t..01.1-th
6 12.2t3-22 r,,,urg sk.S
q VV�
7 12-21-22
.rObn-1
'Or4an'sms fed daey SOUL NC Serenosrrun and SOUL per re ohcate using NandvSteo repeatpi°ettar SN 17E593Si.
Chemical Analyses: Initiation Renewal One Renewal Two
•
Initial Final Initial Final Initial Final
Concentration Analyst Ef... L. . �C .+ ve �G
pH(S.U.) 1.3to -�^C �:.S , a-3I _-4-51.-ttt �.au 7_u2
Dissolved oxygen(mg/l) '},(/) n..C' -1.-I A.2S 1-t. -ta
Conductivity lmhns/cm) 'tJ10 gCfb 3(3
Control,MHSW 'Alkalinity(rndt CaC0,1 `
‘12‘ -- 1 ty Ll.
'Hardness(mg/t CaC0,1 �Sp
.Temperature l'CI "LA.Z t-S.t 1A-8 1S•0 " t'n tS• .--
•
'Aruha idwrifcd for mob day.pert el pH,diunlnd augur and candu_1ivly m°asurmmlrs only Tnepmuao perionred al the rune tallest initiation,rlastaal or lamination by hk
.uuhst identified in the Dady Rera-nal information table.Allalinilr and lucdness performcd hr,the vial,su imlaltirxd on the tot bent!,shorts and transcribed In this batch sheet.
Parameter Reporting Omit Method number !Meier Serial number
PH 0.15.U. 5M 4500.11+D-2011 yammer AR20 9331I452
Oisaoived oxygen 1 0 mg/t SM 4500-0 G-1016 r51 Model S2CE 180104324
Condos-nity 14.9 pmhos/cm SM 2510 e-2011 ARvmel n920 91112452
Alaalinlry 5.0 mg(+COO. 5M:3201-2011 Aacumcr AMC 93312452
lrardn.rs 5.0 mg CaCO,ft SM 2360C-1011 Not a°alleable Not appieab!e
rater re e.t.a Olson. 41 me ORION 97-70-1977 ...rums'A8250 913a9111 L
Temperature 0.1°C 'SM 2550a .+aW10a
-2010 Digital Thermometer 1 ,ta\S
Survival and Reproduction Data:
Observations Replicate number
Day
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality 1.__ t_ t. t•_, L `` t.— t....- k-' l._-
RenewatOne IL•Uwe,D•dead) _ 7' �' ' 1 '' ,.a L . `
Number of broods present 1Lt b t L, eL LQ= L L . a. L L I�Z'
5 Number of young produced t 1 1 -•n 110 t i 7 t \ t l IS t p i 1 1l J 1-
Renewal Two Adult mortality '- ` ` ` r ` L L 1 t_ ` t` LiE•lire.0.dead) 7 { f t '` ' 1
Number of broods present L 1 L L i L l_ LI L L' 1L`... t e. 1=
Number of young produced is tee t-L- t, t l 13 I 1& It. 1 b ty 13
7 Total young produced 2.y` 'L` .Lgs SS ' 3't. 11_ .Ns 3D 33 31 3b
Final 0 i.
Final adult mortality L. L k_ L t.. L- L L s— t_
x for 3rd Broods (• - T x Y- S .
J( $ !(' !- /
Control Acceptance Criteria:
%of Male Adults(5 20%) 01, Mean Offspring/Female .30 GI
%Adults having 3rd Broods(2 80%) 1007e (a 15 offspring/surviving female)
%Mortality(520%1 01. %CV I<40%) 10,11.
SOP AT11Revislon r.Ealub.t AT12.1
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North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0,NC Modification—December 2010,Version 3.0)-Test Bench Sheet
Paired with Control# Z- Date: L2-►'t-1.L
Client Tryon WWTP NPDES# NC 0021601
Facility Outfall 001
Project# t"t5 Crb County Polk
Test Concentration(Chronic Limit) 37% Dilution ml mt. Total volume
preparation: Sample Dilution water nil
Samples were not aerated or treated unless otherwise noted on this form.Control,dilution water 111 189 300
and test renewal information are included on the Control tench Sheet indicated above.
Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst -- ET VC t-t GZ
pHls.u.) - 7 �LDZ —4.(pl SB 1-5'3 3•5C0
Test Dissolved oxygen(mg/LI 1"5 • 1.1 g,t) i .0 —1.4%
Concentration Conductivity(prnhos/cm) 322 325 321
'Temperature(°C) ZS• L ��>•�1 ZS.0 1 S-'L .0
pH(S.u.) _•(f.3 R.(91_
Dissolved oxygen(mg/L) I -t•1
100% Conductivity(pmhos/cm) 352 3'qQ
'Total residual chlorine(mgil) G 0.10 °O•10
Sample number Sample 1 1.1.t.11az f\ sample 2•Z-L1ZlS•o f
'Analyst identified for each day.performed p11.dissolved oxygen and conductivity measurements only Temperatures performed al the since of test initiation.renewal or
terrnootion by the analyst identified in the Daily Renewal Information table located on the Control Bench Slicer Total residual chlorine performed by the analyst identified on
the Total Residual Chlorine Bench Street and transcribed to this tench thee:
Survival and Reproduction Data (performed at test concentration):
Day Observations Replicate number
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality
L_ L
Renewal One IL=L�ivc.O=dead)
L. L L
Number of broods present L� Lt "L L Ll �+LQ2 L L L L
5 Number of young produced (�' l5 tip IS (tp 11t t (A t5 t-\ 1"1 1�
Renewal Twn Adult mortality L. V_ ` L L V- L V_
Il=Live.D.dead)
Number of broods present L► L_ L_ L. L/tt L IL pL L L L Li
Number of young produced Zl� tto Ltp tat tce 1 LS 1g l� ty eta I
7 Total young produced -5c1
Final Jb 'ate S
31 "L bJ 3.!' �
Final adult mortality
IL=Live,D=dead)
Test was Initiated using Sample 1.Sample 2 was used for Renewals One(day 2)and Two(day 5).Samples were diluted to the test concentration prior to use with moderately
hard synthetic water and warmed to 25.0±10'C in a warm water bath.
Comments:
Test Results and Statistical Analyses:
Test results Statistics
%Mortality [-Seat or
V7 Rank Sum -Z'61.4
Mean offspring 1-Tailed 2 s05F
33.E
per female Critical
%Reduction -R.1 7 PASS or FAIL PASS
from control
SOP AT1Z•Revision 4-Exhibit AT12.2
5 of 10 12/28/2022, 10:26 AM

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Qnvi,on enolT..Ung Solution;bu
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1002.0)
Species: Ceriodophnia dubio
C,rrrr.J 4y
II•Y w-•rrnr,
Facility: Tryon WWTP �
I,m L1iia w
Test start date: December 14,2022 •�QN/`
Project number: 17503
Sample numbers: 221213.07,221215.01
Survival Average Coefficient of Percent reduction from
Concentration Number of young produced by replicate number Day (f;) repraductlon variation(%) control(%)
(%) 1 2 3 4 5 6 7 8 9 10 11 12 (offspring/female)
5 14 13 16 16 15 19 17 15 18 17 17 17
Control 7 15 15 12 17 17 13 15 18 12 16 14 13 100 30.9 6.1 Not applicable
Total 29 28 28 33 32 32 32 33 30 33 31 30
5 19 15 16 15 16 18 19 14 15 19 17 16
37% 7 20 16 16 19 18 18 15 18 13 14 16 19 100 33.4 8.1 -8.1
Total 39 31 32 34 34 36 34 32 28 33 33 35
Dunnett's MSD value: 2,39 MSD= Minimum Significant Difference
PMSD: 7.7 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA 1101h percentile) =13%.
Upper PMSD bound determined by USEPA(90"r percentile)=47%.
Lower and upper PMSD bounds were determined from the 10'h and 90ih percentile,respectively,of PMSD data from EPA's WET Interlaboratory
Variability Study(USEPA,2001a;USEPA,2001h). The lower PMSD hound represents a practical limit to the sensitivity of the test method and is
• not a minimum acceptance criteria.
USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-8-01.004 and EPA-821-8-01-005.US
Environmental Protection Agency,Cincinnati,OH.
tJ
IJ
90
lJ
O W
I
F.

Entered by.
H<Ila'y Neenah
Reviewed by:
Statistical Analyses Diener
Enwcanmenul Tecucq SOWr•Onl,Inc. ?aft
Ceriodaphnia Survival and Reproduction Test-Reproduction
Start Date: 12/14/2022 Test ID- CdPFCRNC Sample ID' Tryon
End Date. 12/21/2022 Lab ID. ETS-Envir Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species CD-Ceriodaphnia dubia
Comments: 9 10
Cone-% 1 2 3 4 5 6 7 8
0-Control 29.000 28.000 28 000 33.000 32.000 32.000 32.000 33 000 30 000 33.000
D-Control 31.000 30.000
37 39.000 31.000 32.000 34.000 34.000 36 000 34 000 32.000 28.000 33.000
37 33.000 35.000
Transform:Untransformed 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
D-Control 30.917 1.0000 30.917 28.000 33.000 6.084 12
37 33.417 1.0809 33.417 28.000 39.000 8.116 12 -2.624 2.508 2 390
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution(p>0.01) 0.9769 0.884 -0.041 1.02949
F-Test indicates equal variances(p=0.24) 2.07923 5.31963
Hypothesis Test(1-tail,0.01) MSDu MSDp MSB MSE F-Prob df
Homoscedastic t Test indicates no significant differences 2.38993 0.0773 37 5 5.44697 0.0155 1,22
Treatments vs D-Control
Dose-Response Plot
45-
40
35
30 _ 1-tail,0.01 level
o _ of significance
u 25
v
Q 20
a
d
15
to
5
0
s
0
U
6
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-
MR
t .,_,� 351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Tryon WWTP NPDES#: NC0021601 Pipe tt. 001 County: Polk
Purchase order:
Species: Ceriodapinia dubia Effluent dilution: 37%
Test type: Pass/Fail Chronic Parameter code: TGP3B
I Sample information: (to be completed by sample collector)
Sample location: p LJ21( 6 .. lej,_,ad
Composite sample: P
Start date: /2//2_/2 2 Timc: ()5/00 Volume collected fof sting: qj�X .i.*
/ Time: .)YC C) Number of containers filled For testing. I
End date: / /l3 �2 ' i
Number of samples per hour: 4/ Method of transport to laboratory: / (7, -
Chilled during collection? Ir/ .. Comments: i
If chilled,specify temperature: J
1 Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample collected by:
a I-03/1 z
iPrateDee one wee
r Relioquirhed by: Received by:
/.2/,i/zz 7)/� i�-l3-aa
.x (�sz< 11P�1),c-w/iiUys %ref: tR��.
Dmsed isex
Relinquished by: Received by:
I
Cj k�p C lft� 7
' ��4/ I �
,'L ur C.Rue S Wn< , Lbw see in,e yVRAPb.
l.ie 1.l,' _�e�.,------
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: ^ Received at ETS by:
C rY/!; Sa 1i1322-
',e/ / )3 14ju Ill I ►3i
l.a Spm.ec Om ateleec l'iwa Syu� lauc awl we
Custody seals intact?: n n j Sample temperature upon receipt at ETS CC): , .
Y. w Nee v.N I.
Samples received in good condition?: (] n
.� N. Total residual chlorine upon receipt at ETS: F-1
(DPD Presence/Absence Indicator.MDL o 0.10 ma/L) q''j
Tracking number: �}{� 7 l2434)1'.
Project number!s:5 Sample number:
Comments:
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IMMIF
j + of 351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Tryon WWTP NPDES#: NC0021601 Pipe N: 001 County: Polk
Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 37%
Test type: Pass/Fail Chronic Parameter code: TGP3B
Sample information: (to be completed by sample collector)
Composite sample: Sample location: •.p4 WpAh. e.-t.tooili
Start date: t414/2Z Time: CAS Volume collected fa t Ling:
End date: ja'/S/J2 Time: oc306 Number of containers filled for tin :-7 I
Number of samples per hour: 4 Method of transport to laboratory: v)a.,f
Chilled during collection? tG.a Comments:
If chilled,specify temperature: i
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0"C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample collected by:
ILL- Fl k, wit..44 O tO 0
rev„ aces.Iome,a,�e
Relinquished by: Received by:
1Yl.r-. - 1Y!L , C T�L' C-)CJte i 1 1;=luYfike PZVaAQ 09 0
nasa Do.and tow n,m swewm tnam,aa
IRelinquished by: Received by:
1� K
P,ml arnnme nxa San root Sb,uwc [bte time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
14.0 S.Icn,,.m Roc sod Woke Poet brnamm Dawa.J tow
Custody seals intact?: J 1:=1 �� Sample temperature upon receipt at ETS CC):
Y� No we and .0 C
Samples received in good condition?: n✓
Yes No Total residual chlorine upon receipt at ETS:
1,1p IDPD Presence/Absence Indicator.MDL-0.10 ma/L) P"`"' Ab""
Tracking number: 4vA "��
Project number:{l 91S Sample number:a I5.61
Comments:
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Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
Environmental Testing Solutions.Inc Source: In-house Culture
1.14 I I I I I r t
1.12 - Control Limits (± 2 Standard Deviations) -
•
• •
• -
•
• • •
1.08 — • _ __ Y....__�. • —
1.06 —rts —
1.04 — —
Z -
1.02 — —
I I I I 1 I I 1 I t I I I 1 I I
r`^, - - - - - - - - - - - - - - - I
u
(13 1.15 — Warning Limits
1.10 — : 10—� :.. •_ .' • ...._...v��._ .'^��..�_ .• ..r...
•
•
•
1.05 — -
1.00 —
0.95 1 1 1 I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
0.L%1Q 1 ,LA 1�'OS11 O.L11 01.1�'Op1ti 01'Oa.L1 0�.11'OS11 03.al-0,1.tb,,.L11 0.�,al'13.2ti Oa1ti 0a.al-0$1ti Oy 11
00' p9 09' 10' •NY L2 Ol' 01 0'1: 0 p4- el 06' p9' 00' 09 ,N0 11 11 11.
Test date
• 7-day IC25=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodaphnia reproduction(calculated using ToxCalc).
Central Tendency(mean logarithmic ICZ5 converted to anti-logarithmic values)
- Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
•• •• USEPA Warning Limits (mean logarithmic IC25±SA Io converted to anti-logarithmic values,
SA 10=10th percentile of CVs reported nationally by USEPA)
Itnumr,„
10 of 10 12/28/2022, 10:26 AM