NC0021253_Renewal (Application)_20240520

NORTH Cgg04N
9
'NCORPORATED 1050
April 30, 2024
CITY OF HAVELOCK
Post Office Box 368
1 Governmental Ave.
Havelock, NC 28532
Mr. Mike Montebello
Water Quality Permitting Section — NPDES
Division of Water Resources
North Carolina Department of Environmental Quality
1617 Mail Service Center
Raleigh, NC, 27699 - 617
Re: NPDES Permit Renewal Application (NC0021253)
City of Havelock Wastewater Treatment Plant
City of Havelock, North Carolina
Dear Mr. Montebello:
RECEIVED
MAY 2 0 ZO24
NCDEQ/DWR/NPDES
The City of Havelock Public Services Department is permitted to discharge 2.25 million gallons per day (mgd) of
treated effluent from the City of Havelock Wastewater Treatment Plant (WWTP) to the Neuse River per North
Carolina NPDES Permit NC0021253. The application package for the NPDES permit renewal was submitted to the
Division of Water Resources on December 27, 2023. The renewal application did not include the four required
Second Species Toxicity tests. In the December 2023 correspondence, the City committed to completing the
required testing by the NPDES permit expiration date of June 30, 2024. Attached to this correspondence are the
following revisions to our NPDES renewal application package:
Revised EPA Form Part E, Toxicity Testing Data
• Attachment (Tab G of the December 2023 renewal application package): Second Species Toxicity
Testing Results 2023 - 2024
If you have any questions regarding any of the NPDES permit renewal application materials, please contact me
at [phone] or [e-mail].
S' cerely,
Rick Day
Public Services Director
cc:
Rodney George, City of Havelock
Mary Sadler, PE, Hazen and Sawyer
Jocelyn Russell, PE, Hazen and Sawyer
Todd Davis, PE, Hazen and Sawyer
Phone [phone] www.havelocknc.us Fax [phone]

City of Havelock Wastewater Treatment Plant
City of Havelock, North Carolina
Second Species Toxicity Testing Results 2023 - 2024
RECEIVED
NIA) � o '_�O�4
NC®EQ/®WR/NPDE

�n� r6nmenta.lnc.. [phone] . FAX [phone]
P.O. Box 16414, Greenville- SC 29806 4 Craftsman Court, Greer, SC 29660
Menidia beryllina Survival & Growth Test
EPA-821-R-02-014 Method 1006
Permit Renewal: Second Species Testing
Test Date:
Test Reviewed and Approved By:
Client: CITY OF HAVELOCK
Facility: WWTP
NPDES #: NCO021253
12-Dec-23
Laboratory Sample ID #: T66348
Rohert �e��ec� a�' E
Robert W. Kelley, Ph.D. Amy McMahan
Lab Manager QA/QC Review
lea
AW
AVA
I
Certification#E87819 SCDHEC Certification#23104
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Ccrtification Number E87819
Florida Dept. of Health. Included results pertain only to provided samples. NCDENR Certification # 022
Page 1 of 7

Silverside Chronic Survival and Growth Test - Method 1006
Facility: HnvPlnrk NPDES# NCO021253 Pipe#: 001 County: Craven
Laboratory: ETT Environmental, Inc. Comments
x //
Signature of Operator in Responsible Charge RobeNt Td eL
x U
Sianature of Laboratory Supervisor
% Eff.
Control
3.8
7.5
15.0
30.0
60.0
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of W ater Quality
NC DENR
1621 Mail Service Center
Raleigh,NC 27699-1621
Test Initiatlon Dewrime
12/12/23
/ 04:00 PM
Repl.
1
2
4
Surviving #
Original #
Wt/original (mg)
Surviving #
Original #
Wt/odginal (mg)
Surviving #
Original #
Wt/orginal (mg)
Surviving #
Original #
Wt/original (mg)
Surviving #
Original #
Wt/original (mg)
Surviving #
Original #
Wt/odginal (mg)
Avg WtlSurv. Control 0.4278
4
10
9
10
9
10
9
10
10
0.3340
0.4289
0.3050
0.5790
10
10
10
10
10
10
10
10
0.4130
0.4220
0.5120
0.4730
9
10
9
10
9
10
9
10
0.4389
0.4080
0.4389
0.4760
10
8
10
10
10
9
10
10
0.5340
0.4278
0.4310
0.3750
10
10
10
7
10
10
10
8
0.5490
0.3690
0.4240
0.3400
10
7
10
9
10
9
10
10
0.4120
0.4900
0.5120
0.4140
% Survival
Avg Wt(mg)
0.4117
% Survival
100.0
Avg Wt(mg) F
0.4550
% Survival
100.0%
Avg Wt(mg)
0.4404 71
% Survival
Avg Wt (mg)
0.4419
% Survival
Avg Wt(mg)
0.4205
% Survival
Avg Wt(mg)
0.4570
ED
4
NCDEQ/DWR/NPDES
Test Organisms
R
ultured In -House
utside Supplier
Hatch Date: 12/04
Hatch Time: 1430-1600
Water Quality Data Day
Control 0 1 2 3 4 5 6 7
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
11­11gh Concentration
8.1 / -
8.3 /
8.1
8.2 /
8.0
8.3
/ 8.2
8.2
/ 8.0
8.2 /
8.1
8A /
7.9
- /
7.8
6.4 / -
7.2 /
6.8
7.2 /
6.6
7.3
/ 6.4
7.3
/ 6.3
7.2 /
6.5
6.7 /
6.6
- /
6.7
25.1 1 -
25.0 1
25.1
25.0 1
26.3
25.5
1 25.8
25.4
/ 26.1
25.5 1
26.0
25.0 /
25.1
- /
25.6
a 7
8.2 / -
6.6 / -
8.3
7.0
/ 8.3
/ 6.6
8.2
7.3
/ 8.3
/ 6.7
8.3
7.3
/ 8.3
/ 6.4
8.3
6.6
/ 8.3
/ 6.6
8.2 / 8.2
6.7 / 6.5
8.2 /
6.3 /
7.9
6.0
- /
8.2
25.0 / -
25.0
1 25.1
25.2
1 25.3
25.6
1 25.6
26.5
1 26.1
25.6 / 26.0
252 1
25.1
pH (SU) Init/Fin
DO (mg/L) InitlFin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlodne(mg/L)
Temp at Receipt( C)
1
12/10/23
24 hr
119.1
215.3
716
<0.05
2.0
Dilution H2O
Hardness (mg/L) 93.5
Alkalinity (mg/L) 58.7
Conductivity (umhos/cm) 324
2
2/12123 12/130
24 hr 24 hr
110.6 119.1
183.4 183.0
563 652
0.08 <0.05
0.2 0.1
IWC =15%
Survival Growth Overall Result
Normal 0.86 0.95 ChV > 60
Hom. Var.
inf
6.72
NOEC
60.0%
60.0%
LOEC
> 60.0% >
60.0%
ChV
> 60.0% >
60.0%
Method
Rank Sum
t Test
State
Survival
Growth
Conc.
Crlticei
Calculated
Critical
Calculated
4%
10.00
18.00
2.40
-0.82
6%
10.00
20.00
2.40
-0.54
15%
10.00
17.50
2.40
-0.57
30%
10.00
17.50
2.40
-0.17
60%
10.00
18.00
2.40
-0.85
DWO Form AT-5 (1104)
Page 2 of 7

CHRONIC DEFINITIVE SURVIVAL AND REPRODUCTION/GROWTH TEST
Statistical Analyses
Client: T66348
Sample Identification: Havelock W W TP
Test Date: 12-Dec-2023
Test Species: Menidiaberyllina
Tests for Normality and Heterogeneity of Variance (Growth)
Parameter Test Used Value Result
Sample Use
Sample Date
Sample A 10-Dec-23
Sample B 12-Dec-23
Sample C 14-Dec-23
Sample Used
12-Dec-23 13-Dec-23
14-Dec-23 15-Dec-23
16-Dec-23 17-Dec-23 18-Dec-23
Normality (Growth) Shapiro Wilks 0.954 normal
Variance (Growth) Bartletts 6.72 homogeneous
Normality (Survival) Shapiro Wilks 0.855 normal
Variance Survival Bartletts inf not homogeneous
Tests for Differences in Survival and Reproduction
Test Type Used: Linear Interpolation
Test Concentration % effluent
Effect Control
1 3.8
7.5
15.0
30.0
60.0
survival 97.4%
100.0%
100.0%
97.4%
97.4%
92.3%
% reduction
0.0%
0.0%
1.7%
1.7%
5.9%
Growth Av. wt 0.4117
0.4550
0.4404
0.4419
0.4205
0.4570
m
% reduction smoothed
0.0%
0.0%
0.0%
0.0%
0.0%
Variance 0.0152
0.0021
0.0008
0.0044
0.0086
0.0027
A Criteria Value Upper Limit Lower Lim
V:Coeff. of Variation
30.0%
42.0%
8.9%
?MSD: % MSD
30.4%
9.4%
35.0%
vMD:Min. Sign. Diff.
0.125
Accentabilitv criteria limits not exceeded
IC25 Point Estimate
Reproduction IC25= > 60.0%
TEST RESULTS
%Reduction per Linear Interpolation
@CTC of 15.0%
Ch V& NOEC Using2S% Limit
NOEC Survival 60.0 %
NOEC Growth 60.0 %
ChV Growth >60.0 %
Survival effect 1.7%
Growth effect 0.0%
PASS
Ch V &
NOEC Using
ANOVA
Test Concentration
Comments:
Endpoint
1 3.8
1 7.5
15
1 30
1 60
critical
I ChV
NOEC
tTest
Growth
-0.82
-0.54
-0.57
-0.17
-0.85
2.40
60.oi
60.0%
Outcome
Growth
PASS
PASS
PASS
PASS
PASS
Rank Sum
Survival
18.00
20.00
1 17.50
17.50
18.00
10.00
6o.oi
60.0%
Outcome jSurvival
I PASS
I PASS
I PASS
PASS
PASS
Page 3 of 7

ri
10
9
10
10
10
10
10
10
9
10
9
10
10
9
10
10
10
10
10
8
10
9
10
10
Lab#
T66348
Client
Havelock
Sample ID
WWTP
NPDES#
NCO021253
County
Craven
Month
12
Start 8 fed Date
12-Dec-23
Start 8 fed Time
04:00 PM
Started 8 fed By
AM
Test Organism
Menidia beryllina
Neo. born dale
04-Dec-23
Neo. born time
1430-1600
Test Type
NCCD
Dilution Water
INSTANT OCEAN
Units for Conc.
%
%3rd BROOD
Test vessels
1000 ml
Test volume
500 ml
Incubator #
1
Light
161tl8dk
Elf. Init. Temp'C
25.0
Feeding wf Artemia
.15g xonv
Test method
EPA B21-R-02-013:1...
Comments
All reps have 10
organisms unless noted otherwise.
N
Page 4 of 7

ETT
PO Boa 16414, Greenville, SC 28WJ-7414
[phone], [phone]
Shipping Address: 4 Craftsman Ct, Greer, SC 29650
www2niabArg
CHAIN OF CUSTODY RECORD
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toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours. Equal volui
0.0 and 6.0 *C. Samples must not be frozen. Use water ice in sealed bags. ofsample
collection (completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours.
Sample
may not be used after 72 hours from sample collection.
Flow Proportional: As = instructions in NPDES permit.
Page 5oF7

ETT
Po aox 16414. Gr .WZ-, SC 2SO06-7414
[phone], [phone]
Shipping Address: 4 Craftsman CL Greer, SC 29050
www.ettleharp
CHAIN OF CUSTODY RECORD
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Date Time n ¢d B / 0 izat100 Received 0 / Omanization
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COMPOSITE SAMPLING PROCEDURES
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HOLD TIME PROCEDURES
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Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours. Equal voiw
0.0 and 6.0 eC. Samples must not be frozen. Use water ice in sealed bags.
of sample collection (completion ofcomposite sample).
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flaw Pm rtional: Asper instructions in NPDES permit.
Page A A 7
RECEIVED
Mrs, 2 0 2024
NCDEQ/DWR/NPDES

ETT
PO Box 18414. Greenvale. SC 28a0&7414
[phone]. [phone]
SNpphrg Address: 4 Craftsman Ct, Greer, SC 29650
www-ettlabam
CHAIN OF CUSTODY RECORD
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Date Time Relin uished B / Or ni2alion Received By I Organization
Area
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COMPOSITE SAMPLING PROCEDURES
TEMPERATURE MONIMRING PROCEDURES
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For toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours. Equal voles
0.0 and 6.0 *C. Samples must not be frozen. Use water ice in sealed begs,
of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in NPDES permit.
—_ I. -aye 7 of 7-- --

inv a Inc. [phone] . FAX [phone]
P.O. Box MAU, Greenville, SC 29608 4 Craftsman Courl, Greer, SC.2060
Menidia beryllina Survival and Growth Test
EPA-82 1 -R-02-014 Method 1006
Client: Havelock
Facility: WWTP
NPDES #: NCO021253
Test Date: 23-Jan-24
Laboratory Sample ID #: T66729 MENIDIA
Test Reviewed and Approved By:
e�oheNt 17�e�`ee a"�' E. P'CAah.,Y'
Robert W. Kelley, Ph.D. Amy McMahan
Jab Manager QA/QC Review
Certification#E87819 SCDHEC Certification#23104
Test results presented in this report conform in all requirements of
NELAC, conducted underNELAC Certification Number E87819
Florida Dept. ofaealth. Included results pertain only to provided samples. NC DENR Certification # 022
Page 1 of 7

mrside Chronic Survival and Growth Test - Method 1006
Facility: Havelock WWTP NPDES # NCO021253 Pipe #: 001 County: Craven
Laboratory: - Comments _
x
Signature of Operator in Responsible Charge
x Rokej4 Xe�le,�
Signature of Laboratory Supervisor
% Eff.
Control
3.8
7.5
15.0
60.0
Water Quality Data
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Test Initiation Date/rime 01/23/24 / 04:00 PM Avg Wt/Surv. Control 0.6465
Repl. 1 2 4 4
Surviving #
Original #
WUoriginal (mg)
Surviving #
Original #
WUoriginal (mg)
Surviving #
Original #
WUoriginal (mg)
Surviving #
Original #
Wt/original (mg)
Surviving #
Original #
WUoriginal (mg)
Surviving #
Original #
WUoriginal (mg)
10
10
10
10
10
10
10
10
0.6760
0.6500
0.7460
0.5140
10
9
9
10
10
10
9
10
0.6590
0.6260
0.6633
0.6670
10
10
9
10
10
10
9
10
0.5610
0.5610
0.7433
0.8050
10
10
10
10
10
10
10
10
0.6560
0.7410
0.7650
0.7500
10
10
10
10
10
10
10
10
0.6670
0.6680
0.5790
0.6720
9
10
10
10
9
10
10
10
0.8422
0.7190
0.6020
0.5300
% Survival
100.0
Avg Wt(mg)
0.6465
% Survival
Avg Wt(mg)
0.6538
% Survival
100.0%
Avg Wit (mg)
D.6676
% Survival
100.0%
Avg Wt(mg)
0.7280
% Survival
100.0%
Avg Wt(mg)
0.6465
% Survival
100.0
Avg Wt(mg)
0.6733
Test Organisms
X8Cultured In -House
Outside Supplier
Hatch Date: 01/13
Hatch Time: 1430-1600
Day
Control
0
1
2
3
4
5
6
7
PH (SU) Init/Fin
DO (tr Init/Fin
Temp (C) Init/Fin
8.3 / -
8.1
1 7.7
8.3
/ 8.3
8.0
/ 7.9
8.3
/ 8.1
8.3
/ 8.1
8.2 /
8.0
1--/
8.0
6.9 / -
7.0
/ 6.7
7.0
/ 6.6
5.8
/ 5.5
1 7.0
/ 6.0
6.8
/ 5.4
7.0 /
7.1
- /
6.9
25.1 / -
25.5
/ 26.2
26A
/ 28.1
1 25.3
/ 25.4
25.4
/ 25.7
1 25.1
/ 26.8
25.3 /
25.5
-/
25.4
(High Concentration
PH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhoslem)
Chlorine(mg/L)
Temp at Receipt ( C)
0
1
2
1 2
01/21/24
01/23/24
01/24/24
24 hr
24 hr
24 hr
127.7
126.7
128.9
167.2
158.8
163.9
660
675
686
<0.05
<0.05
<0.05
1.0
0.4
1.5
3 4 5 6 7
8.3
8.2
/ 7.7
8.3
/ 8.5
8.1
/ 8.2
8.2
/ 8.3
8.2
/ 8.3
8.3 /
8.0
- /
8.3
7.2
7.1
/ 6.5
7.5
/ 6.6
6.9
/ 6.0
7.1
/ 6.3
6.6
/ 5.9
6.2 /
7.3
-/
8.5
25.0
25.6
/ 2fi.2
25.8
/ 28.1
25A
/ 25.4
25.1
/ 25.7
25.4
/ 25.8
25.3 /
25.5
- /
45.4
Dilution H2O
Hardness (mg/L)
87.4
Alkalinity (mg/L)
61.8
Conductivity (umhos/cm)
322
Survival
Growth
Overall Result
Normal
0.47
0.95
ChV > 60%
Hom. Var.
inf
10.85
NOEC
60.0%
60.0%
LOEC
> 60.0% >
60.0%
ChV
> 60.0% >
60.0%
Method
Rank Sum
t Test
State
Survival
Growth
Conc.
Critical
Calculated
Critical
Calculated
4%
10.00
16.00
2.40
-0.12
8%
10.00
18.00
2.40
-0.33
15%
10.00
18.00
2.40
-1.28
30%
10.00
18.00
2.40
0.00
60%
10.00
18.00
2.40
-0.42
V6/ rOrm A 1-0 (71D4)
Page 2 of 7

CHRONIC DEFINITIVE SURVIVAL AND REPRODUCTION/GROWTH TEST
Statistical Analyses
Client: T66729 MENIDIA
Sample Identification: Havelock W WTP
Test Date: 23-Jan-2024
;sts for Normality and Heterogeneity of Variance (Growth)
erameter Test Used
Value Result
ormality (Growth) Shapiro Wilks
0.953 normal
ariance (Growth) Bartletts
10.85 homogeneous
ormality (Survival) Shapiro W ilks
0.465 not normal
ariance (Survival) Bartletts
inf not homo$ene
ests for Differences in Survival and Reproduction
Type Used: Linear Interpolation
Test Species: Menidia beryllina
Sample Use
Sample Date Sample Used
ample A 22-Jan-24 23-Jan-24 24-Jan-24
ample B 24-Jan-24 25-Jan-24 26-Jan-24
ample C 26-Jan-24 27-Jan-24 28-Jan-24 29-Jan-24
Test Concentration % effluent
Effect Control
3.8
7.5
15.0
30.0
60.0
survival 100.0%
97.4%
100.0%
100.0%
100.0%
100.0%
% reduction
0.5%
0.5%
0.5%
0.5%
0.5%
Growth Av. wt 0.6465
0.6538
0.6676
0.7280
0.6465
0.6733
m
% reduction smoothed
0.0%
0.0%
0.0%
2.1%
2.1%
Variance 0.0094
0.0004
0.0158
0.0024
0.0020
0.0187
A Criteria Value Upper Limit Lower Limit
CV:Coeff. of Variation
15.0%
42.0%
8.9%
PMSD: % MSD
23.3%
9.4%
35.0%
MSD:Min. Sign. Diff.
0.150
Acceptability criteria limits not exceeded
IC25PointEstimate
Reproduction IC25= > 60.0%
TEST RESULTS
%Reduction per Linear Interpolation
@CTC of 15.0%
Ch V & NOEC Using 25% Limit
NOEC Survival 60.0 %
NOEC Growth 60.0 %
ChV Growth >60.0 /o
Survival effect 0.5%
Growth effect 0.0%
PASS
Ch V & NOEC Usin a ANOVA Test Concentration
Comments:
Endpoint
3.8
1 7.5
15
1 30
60
1 ritical
ChV
NOEC
tTest
Growth
-0.12
-0.33
-1.28
0.00
-0.42
2.40
>6o.o%
60.0%
Outcome
Growth
PASS
PASS
PASS
PASS
PASS
Rank Sum
Survival
16.00
18.00
18.00
18.00
18.00
10.00
>6oi
60.0%
Outcome
I Survival
I PASS
PASS
PASS
PASS
PASS
Page 3 of 7

-------------------
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-------------------
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oria
10
10
10
10
10
10
9
10
10
10
9
10
10
10
10
10
10
10
10
10
9
10
10
10
Lab#
T66729 MENIDIA
Client
Havelock
Sample ID
WWTP
NPDES#
NCO021253
County
Craven
Month
1
Start 8 fed Date
23-Jan-24
Start 8 fed Time
04:00 PM
Started 8 fed By
AM
Test Organism
Menidia beryllina
Neo. born date
13-Jan-24
Neo. born time
1430-1600
Test Type
NCCD
Dilution Water
INSTANT OCEAN
Units for Conc.
%
%3rd BROOD
Test vessels
1000 ml
Test volume
500 ml
Incubator#
1
Light
161U8dk
Elf. Init. Tamp'C
25.0
Feeding w/Artemis
.159 xoAv
Test method IEPA
821-R-02-013:1000
Comments
All reps have 10
organisms unless noted otherwise.
Control ini temp
D=Dead N/A -Lost or not used
Page 4 of 7

RECEIVED
ETT
,PO Box 16414. Greemnlle SC 29OW7414
(B64) 877.6942. [phone]
Shipping Address; 4 Craftsman CL Greer, SC 29650
wee e0lahAM
MAY 20 20Z4
NCDEQID\NR/NPDES
CHAIN OF CUSTODY RECORD
Page —I_of—v—
Client:
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Containers
Preservative
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Facility:
d( +,0"-
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Sample
Date Time Relinquished By I Onganization Received By I Organization
Area
Temp °C
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ljl�.
14 0
AvA o 0 P
COMPOSITE SAMPLING PROCEDURES
TEMPERATURE MONITORING PROCEDURES
HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used within 36 hours
Time Proportional: I sample each hour for 24 hours. Equal volu
0.0 and 6-0'C. Samples must not be frozen. Use hater ice in sealed bags.
of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 2411ours.
Sample may not be used after 72 hours from sample collegian.
Flow Proimel: As instructions in NPDES permit.
Page--

ETT
r—l—W-Q etc �l(
PO Has 16414, Greenville, SC 298n8-7414
[phone], [phone]
Snipping Ad&ess:4 Cra85man Cl, Greer, SC 29650
ww .aW.b m
CHAIN OF CUSTODY RECORD
Page _of_
Client:
ti
Program
Containers
Pr n,oVvc
Parameters
Facility: �.
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Date Time Relin uishedB /0 Organization ReceivedBy/ Or nization
Secure
Area
Receipt
Term, "C
Sample
Preserved?
-e C_
1�6k,5_
COMPOSITE SAMPLING PROCEDURES
Composite samples must be collected over a 24 hour period.
Time Proportional: I sample each hour for 24 hours. Equal volui
or at minimum 1 sample every 4 hours over 24 hours.
Flow Proportional! As Da instructions in NPDES permit.
TEMPERATURC MON/TOR/NG PROCEDURES
Sample temperature during collection and transport must be between
0.0 and 6.0 *C. Samples must not be frozen. Use water ice in sealed bags,
HOLD TIMEPROCEDURES
For toxicity testing the sample must first be used within 36 hours
of sample collection (completion of composite sample).
Sample may not be used after 72 hours from sample collection.
Page 6 cf7

ETT
M - - , �N
, Box 1841" Greenville. SC 298054414
[phone]. [phone]
StOPL-S Addaess: 4 Craftsman Ct, Greer, SC 28850
wwwz dah.arg
CHAIN OF CUSTODY RECORD
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Preserved?
1.'LLA'A
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COMPOSITESAMPLING PROCEDURES
Composite samples must be collected over a 24 hour period.
Time Proportional: I sample each hour for 24 tows. Equal volui
or at minimum 1 sample every 4 hours over 24 hours.
Flow proportional: As per instructions in NPDES permit.
TEMPERATURE MONITORING PROCEDURES
Sample temperature during collection and transport must be between
0.0 and 6.0'C. Samples must not be frozen. Use water ice in sealed bags.
XOLDTIMEPROCEDURES
For toxicity testing the sample must first be used within 36 hours
of sample collection (completion of composite sample).
Sample may not be used after 72 hours from sample collection.
Page 7 of-7—

ilerly ronrteenta , Inc, [phone] . FAX @64j 877-6938
P.O. Sax 16414, Greenville, SC 29606 4 Craftsman Court, Greer, SC 29660
Menidia beryllina Survival and Growth Test
EPA-821-R-02-013 Method 1006
Test Date:
Test Reviewed and Approved By:
Client: Havelock
Facility: WWTP
NPDES #: NCO021253
13-Feb-24
Laboratory Sample ID #: T66874
ecl6ert :Telle� al E-
Robert W. Kelley, Ph.D. Amy McMahan
Lab Manager QA/QC Review
1
Certification #E87819 SCDHEC Certification #23104
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Certification Number E87819
Florida Dept. of Health. Included results pertain only to provided samples. NCDENR Certification # 022
Page 1 of 7

Effluent Toxclty Report Form -Chronic Silverside Mu III -Concentration Test
Facility: Havelock NPDES # NCO021253 Pipe #: 001 County: Craven
Laboratory: - Comments
x // Signature of Operator in Responsible Charge ,[� "rt (�eCle�
x f/� (�
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Test Initiation Date/Time
02/13/24
/ 03:00 PM
Avg Wt/Surv. Control 1.2273
Test Organisms
% Eff.
RepL
1
2
4
4
RI
Cultured In -House
co o-oi
Surviving #
10
10
10
10
%Survival
100.0 %
Outside Supplier
Original #
10
10
10
10
1.2350
1.1880
1.2820
1.2040
10
10
10
10
7.5
Surviving #
%Survival
100.0%
10
10
10
10
Original #
10
10
10
10
10
10
10
9
10
10
10
10
10
10
10
10
8.3
/ -
8.2
8.2
8.1
/ 7.8
8.3
/ 8.0
8.2
/ 8.0
8.3
/ 8.2
8.2 /
7.8
8.0
8.4
8.3
8.4
8.1
/ 8.2
8.4
8.3
8.2
/ 8.2
8.3
/ 8.4
8.2
/ 7.8
8.4
Sample 1 2 Survival Growth Overall Result
Collection Start Date 02/11/24 02/13/24 02/15/24 Normal 0:77 0.92 ChV > 42.5%
Grab Hom. Var. Inf 4.52
Composite (Duration) 24 hr 24 hr 24 hr NOEC 60.0% 30.0%
Hardness (mg/L) 120.8 112.8 123.9 LOEC 60.0% 60.0%>
Alkalinity (mg/L) 183.7 162.1 153.6 ChV 60.0% 42.5%
Conductivity (umhos/cm) 698 604 710 Method Rank Sum It Test
Chlorine(mg/L) <0.05 0.05 <0.05
Temp at Receipt (C) 2.1 0.2 0.8 State Survival Growth
Conc. Critical calculated critical calculated
Dilution H2O 4% 10.00 18.00 2.40 2.90
Hardness (mg/L) 7291.7 8% 10.00 18.00 2.40 2.20
Alkalinity (mg/L) 4.9 15% 10.00 18.00 2.40 3.19
Conductivity (umhos/cm) 55400 30% 10.00 14.00 2.40 1.75
60% 10.00 16.00 2.40 2.52
WQ Form AT-5 (1/04)
Page 2 of 7
WQ Form AT-5 (1/04)
Page 2 of 7

CHRONIC DEFINITIVE SURVIVAL AND REPRODUCTION/GROWTH TEST
Statistical Analyses
Client: T66874 Test Species: Menidia beryllina
Sample Identification: Havelock W W TP
Test Date: 13-Feb-2024
Tests for Normality and Heterogeneity of Variance (Growth)
Sample Use
Parameter Test Used
Value Result
Sample Date
Sample Used
Normality (Growth) Shapiro Wilks
0.924 normal
Sample A
12-Feb-24
13-Feb-24 14-Feb-24
Variance (Growth) Bartletts
4.52 homogeneous
Sample B
14-Feb-24
15-Feb-24 16-Feb-24
Normality (Survival) Shapiro Wilks
0.771 not normal
Sample C
16-Feb-24
17-Feb-24 18-Feb-24 19-Feb-24
Variance Survival Bartletts
inf not homogeneous
ests for Differences in Survival and Reproduction 1 ,
Type Used: Linear Interpolation
Test Concentration % effluent
sffect Control
1 3.8
7.5
15.0
30.0
60.0
iurvival 100.0%
100.0%
100.0%
100.0%
95.0%
97.5%
% reduction
1
0.0%
0.0%
0.0%
3.8%
3.8%
growth Av. %1 1.2273
1.0058
1.0598
0.9840
1.0938
1.0348
mg
% reduction (smoothed)
15.6%
15.6%
15.6%
15.6%
15.7%
Variance 0.0017
0.0131
0.0160
0.0083
0.0245
0.0062
A Criteria Value Upper Limit Lower Limit
:V:Coeff. of Variation
3.4%
42.0%
8.9%
'MSD: % MSD
14.7%
9.4%
35.0%
ISD:Min. Sign. Diff.
0.180
Acceptability criteria limits not exceeded
IC25 Point Estimate
TEST RESULTS
3roduction IC25= > 60.0% %Reduction per Linear Interpolation
V do NOEC Using 25% Limit @CTC of 15.0%
EC Survival >60.0 % Survival effect 0.0%
EC Growth >60.0 % Growth effect 15.6%
V Growth >60.0 io 1 PASS
Page 3 of 7

FIRM
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
9
10
10
10
10
10
10
10
10
Lab#
T66874
Client
Havelock
Sample ID
WWTP
NPDES#
NCO021253
County
Craven
Month
2
Start 8 fed Date
13-Feb-24
Start 8 fed Time
03:00 PM
Started & fed By
AM
Test organism
Menidiabery/lina
Neo. bo rn date
03-Feb-24
Neo. born time
1430-1600
Test Type
NCCD
Dilution Water
Instant Ocean
Units for Conc.
%
Light
16 hr light / 8 hr dark
Test vessels
1000 mL
Test volume
500 mL
Incubator#
1
Cont.Init.Temp°C
25.2
Eff. Init. Temp°C
24.8
Feeding w/Artemia
DayO-3:0.10g/Day4-6:0.15g
Test method
EPA 621-R-02-014:1006
Comments
All reps have 10
organisms unless noted otherwise.
D=Dead N/A -Lost or not used
Page 4 of 7

ETT
,tee-�at�t
'PO Sox 16414, Greemitle, SC 29608-7414
7-6942, (800) 891(864)87-2325
Stripping Address:4 Creasman Cl, Greer, SC 29650
www.ettiahml)
CHAIN OF CUSTODY RECORD
Page _ of
Client: C p 'r J — rL
Progrem
Contalncn
Pmervetivc
Parameters
Facility:
<
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Date Time Relinquished By/ OReceived By / 0 nization
Secure
Area
Receipt
Tem °C
Sample
Preserved?
2. j4.tib,
3.1
la,>/ c ��
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COMPOSITE SAMPLING PROCEDURES
Composite samples must be collected over a 24 hour period.
Time Proportional: l sample each (tour for 24 hours. Equal voles
or at minimum I sample every 4 hours over 24 hours.
Flow Proportional: As per instructions in NPDES permit.
TEMPERATURE MONITORING PROCEDURES
Sample temperature during collection and transport must be bmwen
0.0 and 6.0 °C. Samples must not be frown. Usc hater ice in seated bags.
HOLD TIME PROCEDURES
For toxicity testing the sample must first he used within 36 hours
of sample collection (completion of composite sample).
Sample may not be used after 72 hours from sample collection.
Page 5-of 7

ETT
PO our 16414. Greenville, SC 29OW7414
[phone]. (SM) 891-2325
Shipping Address:4 Craftsman Cl, Greer, SC 29650
wmvEtflah.org
CHAIN OF CUSTODY RECORD
Page _of_
Client: /
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Program
Containers
Preservative
Parameters
Facility: 1 a J _V_
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Nhole EM-1 Toxicity
p
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Date Time Relinquished By/ Oranization Received By/Organization
Area
Tcm aC
Preserved?
,Z.Jy.2►f
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COMPOSITESAMPLING PROCEDURES
TEMPERATURE MONllORING PROCEDURES
HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection end transport must be between
For toxicity testing the sample must first be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volw
0.0 and 6.0 'C. Samples must not be frozen. Use water ice in sealed begs.
ofsample collection (completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Plow Pro ortional: As per instructions in NPDES permit.
-image-€-of 7-

ETT
PO Box 16414. Greem'9e, SC 29806-7414
[phone], (ODD) 881-2325
Shipping AddreW 4 Craftsman Ct. Greer, SC 29850
www.ettlaharg
CHAIN OF CUSTODY RECORD
Page _ of
Client: �,,1 I (-
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S".151
tme 4" Callahan 16,111 of lift✓eldoeC
2 2W5,D.
C011POSITE SAMPLING PROCEDURES
Composite samples must be collected over a 24 hour period.
Time Proportional: 1 sample each hour for 24 hours. Equal volw
or at minimum 1 sample every 4 hours over 24 Hours.
Flow Proportional: As per instructions in NPDES permit.
TEdIPERATURE MONITORING PROCEDURES
Sample temperature during collection and transport must be between
0.0 and 6.0 *C. Samples must not be fiazen. Use hater ice in sealed bags.
HOLD TIME PROCEDURES
For toxicity testing the sample must first be used within 36 hours
of sample collection (completion of composite sample).
Sample may not be used after 72 hours from sample collection.
Page 7-eft --

Env ronrnertta a tne.. [phone] . FAX Y864y 877-6938
RO. Box 16414, Greenville. SC 29805 4 Craftsman Court, Greer, SC 29660
Menidia beryllina Survival and Growth Test
EPA-821-R-02-013 Method 1006
Test Date:
Test Reviewed and Approved By:
G�ohert '5�'ezze y
Robert W. Kelley, Ph.D.
Lab Manager
Client: HAVELOCK
Facility: WWTP EFFLUENT
NPDES #: NCO021253
12-Mar-24
Laboratory Sample ID #: T67082
a,.,,. E. AcA qj.,Y.
Amy McMahan
QA/QC Review
CertificationNE87819 SCDHEC Certification#23104
Ten results presented in this report conform to all requirements of
NELAC, conducted underNELAC Certification Number E97919
Florida Dept. of Health. Included results pertain only to provided sampler. NCDENR Certification 022
Page 1 of 7

Errluent Toxcity Report Form -Chronic Silverside Muill-Concentration Test
Facility: HAVELOCK NPDES # NCO021253 Pipe #: 001 County: Craven
Laboratory: - Comments
x /
Signature of Operator in Responsible Charge
x Gh / ert tJ(�je /ley
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Test Initiation Daterrime
03/12/24
/ 02:50 PM
Avg WUSurv. Control F-6-9-ig-T-1
Test Organisms
% Eff.
Repl.
1
2
4
4
Cultured In -House
�
9
10
10
10
10
10
10
10
7.5
Surviving #
%Survival
10
10
10
10
100.0%
Original #
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
8.1
8.3
8.3
8.1
/ 8.1
8.2
/ 8.3
8.1 /
8.0
8.2 8.0
8.1 /
7.8
- /
7.8
(SU) IniUFin
DO (mg/L) IniVFin
Temp (C) InIVFtn
Sample 1 2 Survival Growth Overall Result
Collection Start Date 03/10/24 03/12/24 03/14/24 Normal 0.47 0.97 ChV > 60
Grab Hom. Var. Inf 2.57
Composite (Duration) 24 hr 24 hr 24 hr NOEC 60.0% 60.0%
Hardness (mg/L) 107.7 118.0 115.7 LOEC > 60.0% > 60.0%
Alkalinity (mg/L) 172.4 181.5 189.1 ChV > 60.0% > 60.0%
Conductivity (umhos/cm) 522 562 586 Method Rank Sum t Test
Chlorine(mg/L) <0.05 <0.05 <0.05
Temp at Receipt (C) 0.4 0.5 0.1 Stals Survival Growth
Conc. Critical Calculated Critical Calculated
Dilution H2O 4% 10.00 26.00 2.40 1.09
Hardness (mg/L) 80.8 8% 10.00 20.00 2.40 2.31
Alkalinity (mg/L) 57.5 15% 10.00 20.00 2.40 1.94
Conductivity (umhos/cm) 318 30% 10.00 20.00 2.40 1.51
60% 10.00 20.00 2.40 1.59
8.2 / - 8.4 / 8.4 8.2 / 8.1 8.3 / 8.4 8.0 / 8.1 7.9 / 8.2 0.0 / 7.8 - / 8.0
- 8.8 / 6.6 6.8 / 5.8 6.0 / 5.9 6.6 / 5.8 6.3 / 5.7 6.5 / 5.7 - / 5.7
1 / 25.1 25.2 I 25.2 24.a / 24.e 24.9 / 25.2 24.9 1 25.5 24.a / 25.1 - / 25.3
7.4
/
U fOrm AI-3 (7/04)
Page 2 of 7

CHRONIC DEFINITIVE SURVIVAL AND REPRODUCTION/GROWTH TEST
Statistical Analyses
Client: T67082 Test Species: Menidia beryllina
Sample Identification: HAVELOCK W WTP EFFLUENT
Test Date: 12-Mar-2024
-sts for Normality and Heterogeneity of Variance (Growth)
arameter
Test Used
Value Result
ormality (Growth)
Shapiro Wilks
0.974 normal
ariance (Growth)
Bartletts
2.57 homogeneous
ormality (Survival) Shapiro Wilks
0.465 not normal
ariance (Survival)
Bartletts
inf not homogene
ests for Differences in Survival and Reproduction
Type Used:
ffect
% reduction
,growth (Av. wt)
% reduction (smooth
Variance
QA Criteria
V:Coeff. of Variatic
'MSD: % MSD
vISD:Min. Sign. Difl
rC25 Point Estimate
Linear Interpolation
Sample Use
Sample Date
Sample Used
ample A
11-Mar-24
12-Mar-24 13-Mar-24
ample B
13-Mar-24
14-Mar-24 15-Mar-24
ample C
15-Mar-24
16-Mar-24 17-Mar-24 18-Mar-24
Test Concentration % effluent
Control
3.8
7.5
15.0
30.0
60.0
97.5%
100.0%
100.0%
100.0%
100.0%
100.0%
0.0%
0.0%
0.0%
0.0%
0.0%
0.8997
0.8185
0.7277
0.7553
0.7873
0.7817
m
ed
9.0%
15.2%
15.2%
15.2%
15.2%
0.0222
0.0174
0.0063
0.0046
1 0.0085
1 0.0073
Value Upper Limit Lower Limit
in
16.6%
42.0%
8.9%
19.5%
9.4%
35.0%
0.175
Acceptability criteria limits not exceeded
production IC25= >
V & NOEC Using 25% Limit
IEC Survival 60.0 %
IEC Growth 60.0 %
V Growth >60.0 /o
EST RESULTS
,Reduction per Linear Interpolation
D,CTC of 15.0%
arvival effect 0.0%
4.5%
PASS
ChV& NOEC Usin ANOVA Test Concentration
Comments:
Endpoint
3.8
7.5
15
30
60
1 critical
ChV
NOEC
t Test
Growth
1.09
1 2.31
1.94
1.51
1.59
2.40
60.o%
60.0%
Outcome
Growth
PASS
PASS
PASS
PASS
PASS
Rank Sum
Survival
26.00
20.00
20.00
20.00
20.00
10.00
>60%
60.0%
Outcome
Survival
PASS
PASS
PASS
PASS
PASS
Page 3 of 7

ricl
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
Lab#
T67082
Client
HAVELOCK
SamplelD
WWTP EFFLUENT
NPDES#
NCO021253
County
Craven
Month
3
Start 8 fed Data
12-Mar-24
Start & fed Time
02:50 PM
Started 3 fed By
AM
Test Organism
Menidia bery/lina
Neo. born date
02-Mar-24
Neo. born time
1430-1600
Test Type
SCCD
Dilution Water
Instant Ocean
Units for Cone.
%..
Light
16 hr light / 8 hr dark
Test vessels
1000 mL
Test volume
500 mL
Incubator#
1
Cont.lnit. Temp°C
24.8
Eff. In it. Temp °C
25.1
Feeding w/Anemia
Day0-3:0.10g/Day4-6:0.15g
Test method
EPA 621-R-02-014:1006
Comments
All reps have 10
organisms unless noted otherwise.
LEtead N/A -Lost or not used
Page 4 of 7

ETTCHAIN OF CUSTODY RECORD
'1'tYYfaL17Fal.4 (Page _of_ I
PO Box 16414, GreemrWe, SC 29OD&7414 1
[phone], [phone]
Shipping Andress 4 Craftsman Cl, Greer, SC 29650
wwwshlab.am
Client: C1}y of- Have[0GK . Provrsm r:nnroieors pr,� Meta. u......,,_._..-
Facility: Waste Wa k_r
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State: N C NPDLS 11: /VC Q
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Test Organisms
SAMPLE ID
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(Composite only)
comp.4eston nek Tlme
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sempk coarrrko oem nma
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Special InsWctions:
Sample Custody Transfer Record
Date Time Relin uished B / Organization Received By I Organization
Secure
Area
Receipt
Tem eC
Sample
Presm-ed?
IY►gBI" C411ahA17 1 01 df HaVebcK
n
COMPOSITE SAMPLING PROCEDURES
TEMPERATURE MONITORING PROCEDURES
HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used Within 36 hours
Time Proportional: I sample each hour for ours. Equal volu
24 h
0.0 and 6.0 *C. Samples must not be frozen. Use Water ice in sealed bags.
of sample collection (completion ofcomposite sample).
or at minimum l sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.erm
Flow Proportional: As per instructions in NPDFS pit
Page_',_

ETT
PO Box 16414, Greamlee, SC 28808-7414
[phone], MCI) 891.2325
Shipping Address: 4 Craftsman Ct, Greer, SC 206M
www Wab.org
CHAIN OF CUSTODY RECORD
Page _of
Client:
T( J0'C—%A
Program
Containers
Preservativc
Parameters
Facility:
I+NAw (1<r(-- �QlaYYc'I�F l.Atir
Whole Emamt Toxicity
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State: NPDRS #:
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Special Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Relit shed B I Or cation Received By I Oreanizalion
Area
Temp °C
Preserved?
Do
¢
0,
COMPOSITE SAMPLING PROCEDURES
TEMPERATURE MONITORING PROCEDURES
HOLD TIMEPROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volw
0.0 and 6.0 *C. Samples must not be frozen Use water ice in sealed bags.
of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in NPDES permit.
6 of-

ETT
PO Box 16414, GreenWOe. SC 29Bn67414
[phone], [phone]
Stdpplm Addrasu 4 Craftsman Cl. Greer, SC 295M
wmiddahnrg
CHAIN OF CUSTODY RECORD
Page of
Client: COY 6f Navaloce
Program
Containers
PreseMtive
Pnramcters
Facllity: waSRVVlIC;,
Whole Emoent Toridry
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State: N G NPDrs#: NC 002 253
A-m
Chronic
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-------------
-------------
-------------
-------------
pecial Instructions:
Sample Custody Transfer Record
Secure
Receipt
Sample
Date Time Relinquished By/ Organization Received By I Organization
Area
Tent oC
Preserved?
3.157-4
is/s
mof an Callahan cio of Havelock
3
COMPOSITE SAMPLING PROCEDURES
TEMPERATURE MONI70RING PROCEDURES
HOLD TIME PROCEDURES
Composite samples must be collected ever a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used amhin 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volut
0.0 and 6.0 *C. Samples must not be frozen. Use \vaer ice in sealed bags.
of sample collection (completion of composite sample).
or at minimum 1 sample every 4 hours over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flow Proportional: As per instructions in NPDES permiL
P

Permit Number Facility Name Outfall Number Form Approved 03I05119
EPA Identification Number =NCO021253
110012588280 City of Havelock WWTP 002 OMB No.2040-0004
TABLEO• FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results.
Test Information
Test Number 1
Test Number 2
Test Number 3
Test species
Mediia beryllina
Mediia beryllina
Mediia beryllina
Age at initiation of test
8 Days
10 Days
10 Days
Outfall number
002
002
002
Date sample collected
12/10/2023 - 12/15/2023
01/21/2024 - 01/26/2024
02/11/2024 - 02/16/2024
Date test started
12/12/2023
01/23/2024
02/13/2024
Duration
7 Days
7 Days
7 Days
Toxicity Test Methods
Test method number
EPA-82-R-02-014:1006
EPA-82-R-02-014:1006
EPA-82-R-02-014:1006
Manual title
Chronic Toxicity of Effluents and Receiving VJ9
Chronic Toxicity of Effluents and Receiving
Chronic Toxicity of Effluents and Receiving
Edition number and year of publication
3rd Edition, 2002
3rd Edition, 2002
3rd Edition, 2002
Page number(s)
All
All
All
Sample Type
Check one:
❑ Grab
0 24-hour composite
❑ Grab
❑✓ 24-hour composite
❑ Grab
24-hour composite
Sample Location
Check one:
❑ Before Disinfection
0 After Disinfection
❑ After Dechlorination
❑ Before Disinfection
❑✓ After Disinfection
❑ After Dechlorination
❑ Before disinfection
0 After disinfection
❑ After dechlorination
Point in Treatment Process
Describe the point in the treatment process
at which the sample was collected for each
test.
Final Effluent
Final Effluent
Final Efflue
0 20 Z
Toxicity Type
Indicate for each test whether the test was
performed to asses acute or chronic toxicity,
or both. (Check one response.)
❑ Acute
❑� Chronic
❑ Both
❑ Acute
0 Chronic
❑Both
❑ Acute
� Chronic
❑Both
EPA Form 3510-2A (Revised 3-19) Page 25
)�S

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05119
110012588280 NCO0212SB City of Havelock WWTP 002 OMB No.2040-0004
TABLE E. EFFLUENT • •• FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results.
Test Number 1
Test Number 2
Test Number 3
Test Type
Indicate the type of test performed. (Check one
response.)
❑ Static
❑� Static -renewal
❑ Flow -through
❑ Static
0 Static -renewal
❑ Flow -through
❑ Static
❑✓ Static -renewal
❑ Flow -through
Source of Dilution Water
Indicate the source of dilution water. (check
one response.)
❑✓ Laboratory water
❑ Receiving water
Laboratory water
❑ Receiving water
Laboratory water
❑ Receiving water
If laboratory water, specify type.
Artificial Salt Water
Artificial Salt Water
Artificial Salt Water
If receiving water, specify source.
Type of Dilution Water
Indicate the type of dilution water. If salt
water, specify "natural' or type of artificial
sea salts or brine used.
❑ Freshwater
Salt water (specify)
Instant Ocean Sea Salt
❑ Fresh water
0 Salt water (specify)
Instant Ocean Sea Salt
❑ Freshwater
0 Salt water (specify)
Instant Ocean Sea Salt
Percentage Effluent Used
Specify the percentage effluent used for all
concentrations in the test series.
3.8, 7.5, 15, 30, 60
3.8, 7.5, 15, 30, 60
3.8, 7.5, 15, 30, 60
0
-
Parameters Tested
Check the parameters tested.
❑✓ pH
❑ Salinity
Temperature
❑ Ammonia
[ Dissolved oxygen
0 pH
❑ Salinity
Temperature
❑ Ammonia
[23 Dissolved oxygen
❑° pH
❑ Salinity
Temperature
❑ Ammonia
Dissolved oxygen
Acute Test Results
Percent survival in 100% effluent
%
%
%
LC5o
95% confidence interval
%
%
%
Control percent survival
%
%
%
EPA Form 3510-2A (Revised 3-19) Page 26

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
110012588280 NCO021253 City of Havelock WWTP 002 OMB No.2040-0004
TABLE E. EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
e table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results.
Test Number 1
i
Test Number 2
Test Number 3
-EW
Acute Test Results Continued
Other (describe)
Chronic Test Results
NOEC
60 %
60 %
> 60 %
IC25
> 60 %
> 60 %
> 60 %
Control percent survival
97.4 %
100 %
100 %
Other (describe)
ChV (chronic value) _ > 60%
ChV (chronic value) _> 60%
ChV (chronic value) _ > 60%
Quality Control/Quality Assurance
Is reference toxicant data available?
0 Yes
❑ No
0 Yes
❑ No
❑✓ Yes
❑ No
Was reference toxicant test within
acceptable bounds?
El Yes
❑ No
Yes
❑ No
❑r Yes
ElNo
What date was reference toxicant test run
MM/DD ?
12/12/2023
01/23/2024
02/13/2024
Other (describe)
EPA Form 3510-2A (Revised 3-19) Page 27

Permit Number Facility Name Outfall Number Form Approved 03/05/19
EPA Identification Number =NCO021253
110012588280 City of Havelock WWTP 002 OMB No.2040-0004
TABLE E. EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results.
Test Information
Test Number 4
Test Number
Test Number
Test species
Mediia beryllina
Age at initiation of test
10 Days
Outfall number
002
Date sample collected
03/10/2024 - 03/15/2024
Date test started
03/12/2024
Duration
7 Days
Toxicity Test Methods
Test method number
EPA-82-R-02-014:1006
Manual title
Chronic Toxicity of Effluents and Receiving
Edition number and year of publication
3rd Edition, 2002
Page number(s)
All
Sample Type
Check one:
❑ Grab
0 24-hour composite
❑ Grab
❑ 24-hour composite
❑ Grab
❑ 24-hour composite
Sample Location
Check one:
❑ Before Disinfection
0 After Disinfection
❑ After Dechlorination
❑ Before Disinfection
❑ After Disinfection
❑ After Dechlorination
❑ Before disinfection
❑ After disinfection
❑ After dechlorination
Point in Treatment Process
Describe the point in the treatment process
at which the sample was collected for each
test.
Final Effluent
Toxicity Type
Indicate for each test whether the test was
performed to asses acute or chronic toxicity,
or both. (check one response.)
❑ Acute
❑✓ Chronic
❑ Both
❑ Acute
El Chronic
El Both
❑ Acute
El Chronic
El Both
EPA Form 3510-2A (Revised 3-19) Page 25

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
110012588280 NCO021253 City of Havelock WWTP 002 OMB No.2040-0004
TABLE E. EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
e table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results.
Test Number 4
i
Test Number
Test Number
Test Type
Indicate the type of test performed. (check one
response.)
❑ Static
Z Static -renewal
❑ Flow -through
❑ Static
❑ Static -renewal
❑ Flow -through
❑ Static
❑ Static -renewal
❑ Flow -through
Source of Dilution Water
Indicate the source of dilution water. (check
one response.)
❑✓ Laboratory water
❑ Receiving water
❑ Laboratory water
❑ Receiving water
❑ Laboratory water
❑ Receiving water
If laboratory water, specify type.
Artificial Salt Water
If receiving water, specify source.
Type of Dilution Water
Indicate the type of dilution water. If salt
water, specify "natural' or type of artificial
sea salts or brine used.
❑ Freshwater
0 Salt water (specify)
instant Ocean Sea Salt
❑ Freshwater
El Salt water (specify)
❑ Freshwater
❑Salt water (specify)
Percentage Effluent Used
Specify the percentage effluent used for all
concentrations in the test series.
3.8, 7.5, 15, 30, 6D
0
Parameters Tested
Check the parameters tested.
❑✓ pH
❑ Salinity
Temperature
❑ Ammonia
Dissolved oxygen
❑ pH
❑ Salinity
❑ Temperature
❑ Ammonia
❑ Dissolved oxygen
❑ pH
❑ Salinity
❑ Temperature
❑ Ammonia
❑ Dissolved oxygen
Acute Test Results
Percent survival in 100% effluent
%
%
%
LC5o
95% confidence interval
%
%
%
Control percent survival
%
%
%
EPA Form 3510-2A (Revised 3-19) Page 26

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
110012588280 NCO021253 City of Havelock WWTP 002 OMB No.2040-0004
TABLE E. EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results.
Test Number 4
Test Number
Test Number
Acute Test Results Continued
Other (describe)
Chronic Test Results
NOEC
60 %
%
%
IC25
> 60 %
%
%
Control percent survival
97.5 %
%
%
Other (describe)
ChV (chronic value) _> 60%
Quality Control/Quality Assurance
Is reference toxicant data available?
❑✓ Yes
❑ No
❑ Yes
❑ No
❑ Yes
❑ No
Was reference toxicant test within
acceptable bounds?
El Yes
❑ No
❑ Yes
❑ No
❑ Yes
❑ No
What date was reference toxicant test run
MM/DD/YYYY ?
03/12/2024
Other (describe)
EPA Form 3510-2A (Revised 3-19) Page 27