NCS000533 DMR SW (Not on Req'd DMR)

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May 4, 2015
NC DENR/DWQ/Environmental Sciences Section
1621 Mail Service Center
Raleigh, NC 27699-1621
RE: 2015 Acute Toxicity Monitoring Report — Form AT -2
To Whom It May Concern:
Enclosed are the three copies of the DWQ Form AT -2 for Acute Pass/Fail forms April 2014 for
Silar Laboratories under Permit NCS000533 for Outfalls SW001, SW002, SW005, and SWO10
TAA313 Acute Toxicity LC50: SW001 No Flow; SW002 Pass; SW005 Fail; SWO10 Fail.
If you have any questions regarding this submittal, I can be contacted at [phone] 101 or
[e-mail]
Regards,
Brandon Sproles
Environmental Safety and Health Manager
Silar Laboratories
333 Neils Eddy Road
Riegelwood, NC 28456
p(910)-655-4212 ext.101
f (910-655-8201)
[e-mail]
300 N. 3rd Street • Suite 120 • Wilmington, NC 28401
RECEIVED
MAY 12 2015
STORMWq ER pQUALITY
ERMIT7ING
�� (p): 910.762.5800 • (f): 910.228.5033 • www.silar.com

© PO Box 7565
O Asheville, NC 28802
O Phone: [phone]
Fax: [phone]
Environmental Testing Solutions, Inc.
Effluent Toxicity Report Form - Acute Pass/Fail Date: April 17, 2015
Facility: Enviro Chemists
Silar Labs
NPDES #: NC- 5000533
Pipe #: SWO02 County: Columbus
Laboratory Performing Test: Environmental Testi olu ' Inc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: Project 10570
Sample 150410.15
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
North Carolina Acute Pass/Fail Toxicity Test
Collection Date: 04-09-15
7.65 7.74
Collection Time: 1012
Test Start Date: 04-10-15
7.61 7.84
Sample/Type/Duration
M
Grab Comp. Duration
Control
3
D.O. (mg/L)
X
Treatment
o cc
X
c°
Alkalinity (mg CaCO3/L'
Hardness (mg CaCO3/L'
Conductivity (µmhos/cm'
Total Residual Chlorine (mg/L;
Sample Temp. at Receipt (°C;
Mortality
34
42
152 97
<0.10
2.4
Organism Tested
48-hour - Ceriodaphina dubia
Control
7.65 7.74
pH (S.U.)
Treatment
7.61 7.84
M
c
v, w
Control
D.O. (mg/L)
Treatment
Replicate
Treatment 1 (Control) A B C D
Treatment 2 (Exposure) A B C D
Concentration 100% 0% 0%
Tested
Note If mean control mortality exceeds ] 0%, the test is considered invalid
Mean Mortality
REC HIVED
MAY 12 2015
DENR-LA Z QUALITY
DRMWAT R PERMITTING
Calculate using Arc -Sine Calculated Student's t NA PASS X
Square Root transformed Tabular Student's t NA FAIL
data. (ONE TAILED)
If the absolute value of the calculated t is less than or equal to the absolute value of the tabular t, check PASS
If the absolute value of the calculated t is greater than the absolute value of the tabular t, check FAIL
If all vessels within each treatment have the same response but the treatment two response is greater than the control, check fail
DWQ form A T-2 (8/91)

• PO Box 7565
Asheville, NC 28802
E
Phone: [phone]
Fax: [phone]
------------ TS
Environmental Testing Solutions, Inc.
Effluent Toxicity Report Form - Acute Pass/Fail Date: April 17, 2015
Facility: Enviro Chemists
Silar Labs
NPDES #: NC- S000533 Pipe #: SWO05 County: Columbus
1
Laboratory Performing Test: Environmental Testi
Signature of Operator in Responsible Charge: <
Signature of Laboratory Supervisor: , / 1 ML
Inc. Comments:
Project. 10570
Sample 15041016
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
North Carolina Acute Pass/Fail Toxicity Test
Mortality
Organism Tested
48-hour - Ceriodaphina dubia
Control
7.65 7.74
pH (S.U.)
Collection Date: 04-09-15
7.21 7.63
v
Collection Time: 1015
Control
D.O. (mg/L)
Test Start Date: 04-10-15
Sample/Type/Duration
iu
Grab Comp. Duration
co
3
X
o
co
X
D
F0
-
Alkalinity (mg CaCO3/L)
34
Hardness (mg CaCO3/L)
42
Conductivity (µmhos/cm)
152
97
Total Residual Chlorine (mg/L)
<0.10
Sample Temp. at Receipt (°C)
2.4
Mortality
Organism Tested
48-hour - Ceriodaphina dubia
Control
7.65 7.74
pH (S.U.)
Treatment
7.21 7.63
v
v, W
Control
D.O. (mg/L)
Treatment
Replicate
Treatment 1 (Control) A B C D
0% 0%11 0% 0%
Treatment 2 (Exposure) A B C D
Concentration H
70% 80% 100% 100%
Tested
Note- If mean control mortality exceeds 10%, thq valid.
Mean Mortality
87.5%
Calculate using Arc -Sine Calculated Student's tPNC�
PASS
Square Root transformed Tabular Student's t FAIL FAIL
data. . - (ONE TAILED)
If the absolute value of the calculated t is less than or equal to the absolute value of the tabular t, check PASS
If the absolute value of the calculated t is greater than the absolute value of the tabular t, check FAIL
If all vessels within each treatment have the same response but the treatment two response is greater than the control, check fail
DWQ form AT -2 (8/91)

PO Box 7565
•
Asheville, NC 28802
Phone: [phone]
OETS Fax: [phone]
Environmental Testing Solutions, Inc.
Effluent Toxicity Report Form - Acute Pass/Fail Date: April 17, 2015
Facility: Enviro Chemists NPDES #: NC- 5000533
Silar Labs
Laboratory Performing Test: Environmental TestingS4utiqus.Inc
Signature of Operator in Responsible Charge: CMUT
Signature of Laboratory Supervisor: (_) L
Pipe #: SW010 County: Columbus
Comments:
Project 10570
Samale 15041017
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
North Carolina Acute Pass/Fail Toxicity Test
Collection Date: 04-09-15
7.65 7.74
pH (S.U.)
Collection Time: 1013
Treatment
6.34 7.22
Test Start Date: 04-10-15
a
v, w
Sample/Type/Duration
D.O. (mg/L)
Grab Comp. Duration
3
X
o
"'
X
O
E°
-
Alkalinity (mg CaCO3/L) 34
Hardness (mg CaCO3/L) 42
Conductivity (µmhos/cm) 152f2.4
Total Residual Chlorine (mg/L)
Sample Temp. at Receipt (°C)
Mortality
Organism Tested
48-hour - Ceriodaphina dubia
Control
7.65 7.74
pH (S.U.)
Treatment
6.34 7.22
a
v, w
Control
D.O. (mg/L)
Treatment
Replicate
Treatment 1 (Control) A B C D
0% 0% 0% 0%
Treatment 2 (Exposure) . A B C D
Concentration H
80% 50% 90% 60%
Tested
Note If mean control mortality exceeds 10%, the test is considered invalid.
Mean Mortality
70%
Calculate using Arc -Sine Calculated Student's t NC PASS
Square Root transformed Tabular Student's t NC FAIL FAIL
data. (ONE TAILED)
If the absolute value of the calculated t is less than or equal to the absolute value of the tabular t, check PASS.
If the absolute value of the calculated t is greater than the absolute value of the tabular t, check FAIL
If all vessels within each treatment have the same response but the treatment two response is greater than the control, check fail
DWQ form AT -2 (8/91)