20240314 Ver 1_Discovery Lake JDR - NIH - Durham NC_20240220

Preliminary Jurisdictional Determination Request
Repair NIH Dam Inlets and Outlets
I I I T.W. Alexander Drive
Durham, NC
Applicant: National Institutes of Health
National Institutes
of Health
Submittal to the U.S. Army Corps of Engineers
September 2023
Agent: A. Morton Thomas and Associates

Appendix 2 - PRELIMINARY JURISDICTIONAL DETERMINATION (PJD) FORM
BACKGROUND INFORMATION
A. REPORT COMPLETION DATE FOR PJD: 06/14/2023
B. NAME AND ADDRESS OF PERSON REQUESTING PJD:
C. DISTRICT OFFICE, FILE NAME, AND NUMBER:
D. PROJECT LOCATION(S) AND BACKGROUND INFORMATION:
(USE THE TABLE BELOW TO DOCUMENT MULTIPLE AQUATIC RESOURCES AND/OR
AQUATIC RESOURCES AT DIFFERENT SITES)
State: NC County/parish/borough: Durham City: Durham
Center coordinates of site (lat/long in degree decimal format):
Lat.: 35.885770 Long.:-78.875997
Universal Transverse Mercator: 17S 691689 3973122
Name of nearest waterbody: Discovery Lake
E. REVIEW PERFORMED FOR SITE EVALUATION (CHECK ALL THAT APPLY):
❑ Office (Desk) Determination. Date:
❑ Field Determination. Date(s):
TABLE OF AQUATIC RESOURCES IN REVIEW AREA WHICH "MAY BE" SUBJECT TO REGULATORY
JURISDICTION.
Site
number
Latitude
(decimal
degrees)
Longitude
(decimal
degrees)
Estimated amount
of aquatic resource
in review area
(acreage and linear
feet, if applicable)
Type of aquatic
resource (i.e., wetland
vs. non -wetland
waters)
Geographic authority
to which the aquatic
resource "may be"
subject (i.e., Section
404 or Section 10/404)
EE ADDI
ONAL SHE
T

TABLE OF AQUATIC RESOURCES IN REVIEW AREA WHICH "MAY BE"
SUBJECT TO REGULATORY JURISDICTION - LAKE DISCOVERY
Site
Number
Latitude
(decimal degrees)
Longitude
(decimal degrees)
Estimated amountType
of aquatic resource
in review area (sq.ft.)
of aquatic
resource
Geographic
authority
PFO #1
35.886836
-78.876561
17,746
PFO wetland
10/404
R3 #1
35.886422
-78.87613
530
non -wetland
waters R3
10/404
LEM #1
35.885652
-78.875685
3,949
LEM wetland
10/404
LEM/LSS #1
35.885451
-78.874764
1,748
LEM/LSS wetland
10/404
LEM/LSS #2
35.884776
-78.874093
4,592
LEM/LSS wetland
10/404
LEM/LSS #3
35.884676
-78.875348
26,874
LEM/LSS wetland
10/404
LEM/LSS #4
35.883022
-78.87582
1,805
LEM/LSS wetland
10/404
LEM/LSS #5
35.882312
-78.875614
4,822
LEM/LSS wetland
10/404
LEM #2
35.880724
-78.875465
7,370
LEM wetland
10/404
LEM/LSS #6
35.880216
-78.873868
1,216
LEM/LSS wetland
10/404
LEM/LSS #7
35.880322
-78.876907
2,633
LEM/LSS wetland
10/404
LUB #1
35.883664
-78.876449
1,082,291
non -wetland
waters LUB
10/404

1) The Corps of Engineers believes that there may be jurisdictional aquatic resources in
the review area, and the requestor of this PJD is hereby advised of his or her option
to request and obtain an approved JD (AJD) for that review area based on an
informed decision after having discussed the various types of JDs and their
characteristics and circumstances when they may be appropriate.
2) In any circumstance where a permit applicant obtains an individual permit, or a
Nationwide General Permit (NWP) or other general permit verification requiring "pre -
construction notification" (PCN), or requests verification for a non -reporting NWP or
other general permit, and the permit applicant has not requested an AJD for the
activity, the permit applicant is hereby made aware that: (1) the permit applicant has
elected to seek a permit authorization based on a PJD, which does not make an
official determination of jurisdictional aquatic resources; (2) the applicant has the
option to request an AJD before accepting the terms and conditions of the permit
authorization, and that basing a permit authorization on an AJD could possibly result
in less compensatory mitigation being required or different special conditions; (3) the
applicant has the right to request an individual permit rather than accepting the terms
and conditions of the NWP or other general permit authorization; (4) the applicant can
accept a permit authorization and thereby agree to comply with all the terms and
conditions of that permit, including whatever mitigation requirements the Corps has
determined to be necessary; (5) undertaking any activity in reliance upon the subject
permit authorization without requesting an AJD constitutes the applicant's acceptance
of the use of the PJD; (6) accepting a permit authorization (e.g., signing a proffered
individual permit) or undertaking any activity in reliance on any form of Corps permit
authorization based on a PJD constitutes agreement that all aquatic resources in the
review area affected in any way by that activity will be treated as jurisdictional, and
waives any challenge to such jurisdiction in any administrative or judicial compliance
or enforcement action, or in any administrative appeal or in any Federal court; and (7)
whether the applicant elects to use either an AJD or a PJD, the JD will be processed
as soon as practicable. Further, an AJD, a proffered individual permit (and all terms
and conditions contained therein), or individual permit denial can be administratively
appealed pursuant to 33 C.F.R. Part 331. If, during an administrative appeal, it
becomes appropriate to make an official determination whether geographic
jurisdiction exists over aquatic resources in the review area, or to provide an official
delineation of jurisdictional aquatic resources in the review area, the Corps will
provide an AJD to accomplish that result, as soon as is practicable. This PJD finds
that there "may be"waters of the U.S. and/or that there "may be" navigable waters of
the U.S. on the subject review area, and identifies all aquatic features in the review
area that could be affected by the proposed activity, based on the following
information:

SUPPORTING DATA. Data reviewed for PJD (check all that apply)
Checked items should be included in subject file. Appropriately reference sources
below where indicated for all checked items:
■❑ Maps, plans, plots or plat submitted by or on behalf of the PJD requestor:
Map:
0 Data sheets prepared/submitted by or on behalf of the PJD requestor.
❑ Office concurs with data sheets/delineation report.
❑ Office does not concur with data sheets/delineation report. Rationale:
❑ Data sheets prepared by the Corps:
❑ Corps navigable waters' study:
❑ U.S. Geological Survey Hydrologic Atlas:
❑ USGS NHD data.
❑ USGS 8 and 12 digit HUC maps.
■❑ U.S. Geological Survey map(s). Cite scale & quad name:
■❑ Natural Resources Conservation Service Soil Survey. Citation:
■❑ National wetlands inventory map(s). Cite name:
❑ State/local wetland inventory map(s):
■❑ FEMA/FIRM maps:
El
■❑
100-year Floodplain Elevation is: n/a
Photographs: ❑ Aerial (Name & Date):
or ❑ Other (Name & Date):
(National Geodetic Vertical Datum of 1929)
❑ Previous determination (s). File no. and date of response letter:
❑ Other information (please specify):
IMPORTANT NOTE: The information recorded on this form has not necessarily
been verified by the Corps and should not be relied upon for later jurisdictional
determinations.
Signature and date of
Regulatory staff member
completing PJD
Signature and date of
person requesting PJD
(REQUIRED, unless obtaining
the signature is impracticable)'
' Districts may establish timeframes for requestor to return signed PJD forms. If the requestor does not respond
within the established time frame, the district may presume concurrence and no additional follow up is
necessary prior to finalizing an action.

urisdictional Determination Reauest
US Army Corps
of Engineers
Wilmington District
This form is intended for use by anyone requesting a jurisdictional determination (JD) from the
U.S. Army Corps of Engineers, Wilmington District (Corps). Please include all supporting
information, as described within each category, with your request. You may submit your request
via mail, electronic mail, or facsimile. Requests should be sent to the appropriate project
manager of the county in which the property is located. A current list of project managers by
assigned counties can be found on-line at:
http://www.saw.usace.gr y.mil/Missions/Re u_ 1�atoiyPermitProgram/Contact/Coun!yLocator.aspx
by calling 910-251-4633, or by contacting any of the field offices listed below. Once your
request is received you will be contacted by a Corps project manager.
ASHEVILLE & CHARLOTTE REGULATORY
FIELD OFFICES
US Army Corps of Engineers
151 Patton Avenue, Room 208
Asheville, North Carolina 28801-5006
General Number: [phone]
Fax Number: [phone]
RALEIGH REGULATORY FIELD OFFICE
US Army Corps of Engineers
3331 Heritage Trade Drive, Suite 105
Wake Forest, North Carolina 27587
General Number: [phone]
Fax Number: [phone]
INSTRUCTIONS:
WASHINGTON REGULATORY FIELD OFFICE
US Army Corps of Engineers
2407 West Fifth Street
Washington, North Carolina 27889
General Number: [phone]
FaxNumber: [phone]
WILNIINGTONREGULATORY FIELD OFFICE
US Army Corps of Engineers
69 Darlington Avenue
Wilmington, North Carolina 28403
General Number: 910-251-4633
Fax Number: [phone]
All requestors must complete Parts A, B, C, D, E, F and G.
NOTE TO CONSULTANTS AND AGENCIES: If you are requesting a JD on behalf of a
paying client or your agency, please note the specific submittal requirements in Part H.
NOTE ON PART D — PROPERTY OWNER AUTHORIZATION: Please be aware that
all JD requests must include the current property owner authorization for the Corps to
proceed with the determination, which may include inspection of the property when
necessary. This form must be signed by the current property owner(s) or the owner(s)
authorized agent to be considered a complete request.
NOTE ON PART D - NCDOT REQUESTS: Property owner authorization/notification for
JD requests associated with North Carolina Department of Transportation (NCDOT)
projects will be conducted according to the current NCDOT/USACE protocols.
NOTE TO USDA PROGRAM PARTICIPANTS: A Corps approved or preliminary JD
may not be valid for the wetland conservation provisions of the Food Security Act of
1985. If you or your tenant are USDA Program participants, or anticipate participation in
USDA programs, you should also request a certified wetland determination from the local
office of the Natural Resources Conservation Service, prior to starting work.
Version: May 2017 Page 1

A.
B.
C.
Jurisdictional Determination Request
PARCEL INFORMATION
Street Address: 111 TW Alexander Dr.
City, State:
County:
Durham
Parcel Index Number(s) (PIN): 0737-66-44-1652
REQUESTOR INFORMATION
Name:
John Farrell - AMT and Assoc.
Mailing Address:
100 Gateway Center Parkway, # 200
North Chesterfield, VA 23235
Telephone Number:
804-335-5603
Electronic Mail Address:
[e-mail]
Select one:
❑ I am the current property owner.
✓❑ I am an Authorized Agent
or Environmental Consultanti
❑ Interested Buyer or Under Contract to Purchase
❑ Other, please explain.
PROPERTY OWNER INFORMATION
Name:
National Institutes of Health
Mailing Address:
111 TW Alexander Dr.
Durham, NC 27709
Telephone Number:
[phone]
Electronic Mail Address:
[e-mail]
' Must provide completed Agent Authorization Form/Letter.
2 Documentation of ownership also needs to be provided with request (copy of Deed, County GIS/Parcel/Tax Record).
Version: May 2017 Page 2

Jurisdictional Determination Request
D. PROPERTY ACCESS CERTIFICATIONS,4
By signing below, I authorize representatives of the Wilmington District, U.S. Army Corps of
Engineers (Corps) to enter upon the property herein described for the purpose of conducting on -
site investigations, if necessary, and issuing a jurisdictional determination pursuant to Section
404 of the Clean Water Act and/or Section 10 of the Rivers and Harbors Act of 1899. I, the
undersigned, am either a duly authorized owner of record of the property identified herein, or
acting as the duly authorized agent of the owner of record of the property.
Print Name
Capacity: ❑ Owner ❑ Authorized Agents
Date
Signature
E. REASON FOR JD REQUEST: (Check as many as applicable)
❑ I intend to construct/develop a projector perform activities on this parcel which would be
designed to avoid all aquatic resources.
❑ I intend to construct/develop a projector perform activities on this parcel which would be
designed to avoid all jurisdictional aquatic resources under Corps authority.
✓❑ I intend to construct/develop a projector perform activities on this parcel which may
require authorization from the Corps, and the JD would be used to avoid and minimize
impacts to jurisdictional aquatic resources and as an initial step in a future permitting
process.
❑ I intend to construct/develop a projector perform activities on this parcel which may
require authorization from the Corps; this request is accompanied by my permit application
and the JD is to be used in the permitting process.
❑ I intend to construct/develop a projector perform activities in a navigable water of the
U.S. which is included on the district Section 10 list and/or is subject to the ebb and flow of
the tide.
A Corps JD is required in order obtain my local/state authorization.
I intend to contest jurisdiction over a particular aquatic resource and request the Corps
confirm that jurisdiction does/does not exist over the aquatic resource on the parcel.
❑ I believe that the site may be comprised entirely of dry land.
❑ Other:
3 For NCDOT requests following the current NCDOT/USACE protocols, skip to Part E.
4 If there are multiple parcels owned by different parties, please provide the following for each additional parcel on a
continuation sheet.
5 Must provide agent authorization form/letter signed by owner(s).
Version: May 2017 Page 3

Jurisdictional Determination Request
F. JURISDICTIONAL DETERMINATION (JD) TYPE (Select One)
❑✓ I am requesting that the Corps provide a preliminary JD for the property identified herein.
A Preliminary Jurisdictional Determination (PJD) provides an indication that there may
be "waters of the United States" or "navigable waters of the United States"on a property.
PJDs are sufficient as the basis for permit decisions. For the purposes of permitting, all
waters and wetlands on the property will be treated as if they are jurisdictional "waters of
the United States". PJDs cannot be appealed (33 C.F.R. 331.2); however, a PJD is
"preliminary" in the sense that an approved JD can be requested at any time. PJDs do
not expire.
❑ I am requesting that the Corps provide an approved JD for the property identified herein.
An Approved Jurisdictional Determination (AJD) is a determination that
jurisdictional "waters of the United States" or "navigable waters of the United
States" are either present or absent on a site. An approved JD identifies the limits of
waters on a site determined to be jurisdictional under the Clean Water Act and/or
Rivers and Harbors Act. Approved JDs are sufficient as the basis for permit
decisions. AJDs are appealable (33 C.F.R. 331.2). The results of the AJD will be
posted on the Corps website. A landowner, permit applicant, or other "affected
party" (33 C.F.R. 331.2) who receives an AJD may rely upon the AJD for five years
(subject to certain limited exceptions explained in Regulatory Guidance Letter 05-
02).
❑ I am unclear as to which JD I would like to request and require additional information
to inform my decision.
G. ALL REQUESTS
Map of Property or Project Area. This Map must clearly depict the boundaries of the
review area.
✓� Size of Property or Review Area 54.8 acres.
❑✓ The property boundary (or review area boundary) is clearly physically marked on the site.
Version: May 2017 Page 4

Jurisdictional Determination Request
H. REQUESTS FROM CONSULTANTS
Project Coordinates (Decimal Degrees): Latitude: 35.885770
Longitude:-78.8759-3T_
A legible delineation map depicting the aquatic resources and the property/review area.
Delineation maps must be no larger than 1 Ix 17 and should contain the following: (Corps
signature of submitted survey plats will occur after the submitted delineation map has been
reviewed and approved).'
■ North Arrow
■ Graphical Scale
■ Boundary of Review Area
■ Date
■ Location of data points for each Wetland Determination Data Form or tributary
assessment reach.
For Approved Jurisdictional Determinations:
■ Jurisdictional wetland features should be labeled as Wetland Waters of the US, 404
wetlands, etc. Please include the acreage of these features.
■ Jurisdictional non -wetland features (i.e. tidal/navigable waters, tributaries,
impoundments) should be labeled as Non -Wetland Waters of the US, stream, tributary,
open water, relatively permanent water, pond, etc. Please include the acreage or linear
length of each of these features as appropriate.
■ Isolated waters, waters that lack a significant nexus to navigable waters, or non -
jurisdictional upland features should be identified as Non -Jurisdictional. Please
include a justification in the label regarding why the feature is non jurisdictional (i.e.
"Isolated", "No Significant Nexus", or "Upland Feature"). Please include the acreage
or linear length of these features as appropriate.
For Preliminary Jurisdictional Determinations:
Wetland and non -wetland features should not be identified as Jurisdictional, 404,
Waters of the United States, or anything that implies jurisdiction. These features can be
identified as Potential Waters of the United States, Potential Non -wetland Waters of
the United States, wetland, stream, open water, etc. Please include the acreage and
linear length of these features as appropriate.
Completed Wetland Determination Data Forms for appropriate region
(at least one wetland and one upland form needs to be completed for each wetland type)
6 Please refer to the guidance document titled "Survey Standards for Jurisdictional Determinations" to ensure that the
supplied map meets the necessary mapping standards. hlW://www.saw.usace.army.mil/Missions/Regulatoiy-Permit-
Pro gram/Jurisdiction/
Version: May 2017 Page 5

Jurisdictional Determination Request
✓❑ Completed appropriate Jurisdictional Determination form
• PJDs, please complete a Preliminary Jurisdictional Determination Form' and include the
Aquatic Resource Table
• AJDS, please complete an Approved Jurisdictional Determination Form'.
✓❑ Vicinity Map
❑✓ Aerial Photograph
❑ USGS Topographic Map
Soil Survey Map
zOther Maps, as appropriate (e.g. National Wetland Inventory Map, Proposed Site
Plan, previous delineation maps, LIDAR maps, FEMA floodplain maps)
zLandscape Photos (if taken)
NCSAM and/or NCWAM Assessment Forms and Rating Sheets
❑ NC Division of Water Resources Stream Identification Forms
Other Assessment Forms
7 www.saw.usace.army.mil/Portals/59/docs/regulatorv/regdocs/JD/RGL_08-02_App A Prelim JD Form fillable.pdf
s Please see http://www.saw.usace.army.mil/Missions/Regulatoa-Permit-Program/Juiisdiction%
Principal Purpose: The information that you provide will be used in evaluating your request to determine
whether there are any aquatic resources within the project area subject to federaljurisdiction under the regulatory
authorities referenced above.
Routine Uses: This information may be shared with the Department of Justice and other federal, state, and local
government agencies, and the public, and may be made available as part of a public notice as required by federal
law. Your name and property location where federal jurisdiction is to be determined will be included in the
approved jurisdictional determination (AJD), which will be made available to the public on the District's website
and on the Headquarters USAGE website.
Disclosure: Submission of requested information is voluntary; however, if information is not provided, the
request for an AJD cannot be evaluated nor can an AJD be issued.
Version: May 2017 Page 6

Figure 1 - Vicinity Map
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Figure 4 - NRCS Durham County Soils Map
Soil Map —Durham County, North Carolina
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A MORTON THOMAS AND ASSOCIATES, INC.
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100 Gateway Center Parkway, Suite 200
North Chesterfield, VA 23235
PHONE [phone]
EMAIL: [e-mail]
REPAIRS NIH DAM
INLETS AND OUTLETS
TASK ORDER C100809
DAM INVENTORY# DURHA -105
FEDERAL NID # NC01666
DURHAM COUNTY, NC
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NATIONAL INSTITUTES OF HEALTH
111 T.W. ALEXANDER DRIVE
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4RK DATE DESCRIPTION
SCALE: 1'_'0'
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DRAWN BY: AMT
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-- — — — — — -- SHEETTITLE
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LEM Wetland - 0259 Ac.
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A- MORTON THOMAS AND ASSOCIATES, INC.
CONSULTING ENGINEERS
100 Gateway Center Parkway, Suite 200
North Chesterfield, VA 23235
PHONE [phone]
EMAIL: [e-mail]
REPAIRS NIH DAM
INLETS AND OUTLETS
TASK ORDER C100809
DAM INVENTORY# DURWk105
FEDERAL NID # NCO1666
DURHAM COUNTY, NC
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NATIONAL INSTITUTES OF HEALTH
111 T.W. ALEXANDER DRIVE
DURHAM, NC 27709
[phone]
DESCRIPTION
SCALE: 1"=30'
DESIGNED BY: AMT
DRAWN BY: AMT
Waters of the U.S.
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A-MORTON THOMAS AND ASSOCIATES, INC
CONSULTING ENGINEERS
100 Gateway Center Parkway, Suite 200
North Chesterfield, VA 23235
PHONE [phone]
EMAIL: [e-mail]
REPAIRS NIH DAM
INLETS AND OUTLETS
TASK ORDER C100809
DAM INVENTORY# DURHA -105
FEDERAL NID # NC01666
DURHAM COUNTY, NC
N I H)j
NATIONAL INSTITUTES OF HEALTH
111 T.W. ALEXANDER DRIVE
DURHAM, NC 27709
[phone]
DESCRIPTION
SCALE: 1`7
DESIGNED BY: AMT
DRAWN BY: AMT
Waters of the U.S.
and Wetlands
Delineation Exhibit
JDR-5

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REPAIRS NIH DAM
INLETS AND OUTLETS
TASK ORDER C100809
DAM INVENTORY# DURHAk105
FEDERAL NID # NC01666
DURHAM COUNTY, NC
NIH
I NATIONAL INSTITUTES OF HEALTH I
111 T.W. ALEXANDER DRIVE
DURHAM, NC 27709
[phone]
■ MARK I DATE I DESCRIPTION ■
SCALE:
DESIGNED BY:
DRAWN BY:
JDR-6

U.S. Army Corps of Engineers OMB Control #: 0710-0024, Exp:1113012024
WETLAND DETERMINATION DATA SHEET— Eastern Mountains and Piedmont Region Requirement Control Symbol EXEMPT:
See ERDC/EL TR-12-9; the proponent agency is CECW-CO-R (Authority: AR 335-15, paragraph 5-2a)
Project/Site: Discovery Lake City/County: Durham Sampling Date: 11/02/22
Applicant/Owner: National Institutes of Health State: NC Sampling Point: UPL #1
Investigator(s): J. Farrell - AMT Section, Township, Range:
Landform (hillside, terrace, etc.): face of dam Local relief (concave, convex, none): concave Slope (%): 20
Subregion (LRR or MLRA): LRR P, MLRA 136 Lat: 35.886136 Long:-78.875725 Datum: NAD 83
Soil Map Unit Name: White Store sandy loam NWI classification: UPL
Are climatic / hydrologic conditions on the site typical for this time of year? Yes X No (If no, explain in Remarks.)
Are Vegetation Soil or Hydrology significantly disturbed? Are "Normal Circumstances" present? Yes X No
Are Vegetation Soil or Hydrology naturally problematic? (If needed, explain any answers in Remarks.)
SUMMARY OF FINDINGS — Attach site map showing sampling point locations, transects, important features, etc.
Hydrophytic Vegetation Present? Yes No X Is the Sampled Area
Hydric Soil Present? Yes No X within a Wetland? Yes No X
Wetland Hydrology Present? Yes No X
Remarks:
HYDROLOGY
Wetland Hydrology Indicators:
Secondary Indicators (minimum of two required)
Primary Indicators (minimum of one is required: check all that apply)
—Surface Soil Cracks (136)
—Surface Water (Al) —True
Aquatic Plants (1314)
—Sparsely Vegetated Concave Surface (138)
_ High Water Table (A2) —Hydrogen
Sulfide Odor (Cl)
_ Drainage Patterns (1310)
—Saturation (A3) —Oxidized
Rhizospheres on Living Roots (C3)
—Moss Trim Lines (1316)
—Water Marks (131) —Presence
of Reduced Iron (C4)
_ Dry -Season Water Table (C2)
_Sediment Deposits (132) _Recent
Iron Reduction in Tilled Soils (C6)
_Crayfish Burrows (C8)
_ Drift Deposits (133) _Thin
Muck Surface (C7)
—Saturation Visible on Aerial Imagery (C9)
_Algal Mat or Crust (134) _Other
(Explain in Remarks)
_Stunted or Stressed Plants (D1)
—Iron Deposits (135)
_ Geomorphic Position (D2)
_ Inundation Visible on Aerial Imagery (137)
—Shallow Aquitard (D3)
—Water-Stained Leaves (139)
_ Microtopographic Relief (D4)
Aquatic Fauna (1313)
FAC-Neutral Test (D5)
Field Observations:
Surface Water Present? Yes No
X Depth (inches):
Water Table Present? Yes No
X Depth (inches):
Saturation Present? Yes No
X Depth (inches):
Wetland
Hydrology Present? Yes No X
(includes capillary fringe)
Describe Recorded Data (stream gauge, monitoring well, aerial photos, previous inspections), if available:
Remarks:
Sample location Is the face of the dam
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

VEGETATION (Four Strata) - Use scientific names of plants.
Sampling Point: UPL #1
Absolute Uominant Indicator
Tree Stratum
(Plot size: )
% Cover Species? Status
Dominance Test worksheet:
1
Number of Dominant Species
2.
That Are OBL, FACW, or FAC: 0 (A)
3.
Total Number of Dominant
4.
Species Across All Strata: 1 (B)
5.
Percent of Dominant Species
6.
That Are OBL, FACW, or FAC: 0.0% (A/B)
7.
Prevalence Index worksheet:
=Total Cover
Total % Cover of: Multiply by:
50% of total cover:
20% of total cover:
OBL species 0 x 1 = 0
Sapling/Shrub Stratum (Plot size:
)
FACW species 0 x 2 = 0
1.
FAC species 0 x 3 = 0
2.
FACU species 0 x 4 = 0
3.
UPL species 0 x 5 = 0
4.
Column Totals: 0 (A) 0 (B)
5.
Prevalence Index = B/A =
6.
Hydrophytic Vegetation Indicators:
7.
_ 1 - Rapid Test for Hydrophytic Vegetation
8.
2 - Dominance Test is >50%
9.
3 - Prevalence Index is !2.01
=Total Cover
4 - Morphological Adaptations' (Provide supporting
50% of total cover:
20% of total cover:
data in Remarks or on a separate sheet)
Herb Stratum
(Plot size: 40'x40' )
_ Problematic Hydrophytic Vegetation' (Explain)
1. fescue
100 Yes
Indicators of hydric soil and wetland hydrology must
2.
be present, unless disturbed or problematic.
Definitions of Four Vegetation Strata:
3.
4.
Tree - Woody plants, excluding vines, 3 in. (7.6 cm) or
5.
more in diameter at breast height (DBH), regardless of
6
height.
7.
Sapling/Shrub - Woody plants, excluding vines, less
8.
than 3 in. DBH and greater than or equal to 3.28 ft
9
(1 m) tall.
10.
Herb - All herbaceous (non -woody) plants, regardless
11.
of size, and woody plants less than 3.28 ft tall.
100 =Total Cover
Woody Vine - All woody vines greater than 3.28 ft in
50% of total cover:
50 20% of total cover: 20
height.
Woody Vine Stratum (Plot size: )
1.
2.
3.
4.
5.
Hydrophytic
=Total Cover
Vegetation
50% of total cover:
20% of total cover:
Present? Yes No X
Remarks: (Include photo numbers here or on a separate sheet.)
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont - Version 2.0

SOIL Sampling Point: UPL #1
Profile Description: (Describe to the depth needed to document the indicator or confirm the absence of indicators.)
Depth Matrix Redox Features
(inches) Color (moist) % Color (moist) % Type LoC2 Texture Remarks
0-12 5YR 5/6 100 Loamy/Clayey dam fill
Type: C=Concentration, D=Depletion, RM=Reduced Matrix, MS=Masked Sand Grains.
Hydric Soil Indicators:
_ Histosol (Al)
_ Histic Epipedon (A2)
_ Black Histic (A3)
—Hydrogen Sulfide (A4)
—Stratified Layers (A5)
2 cm Muck (A10) (LRR N)
_ Depleted Below Dark Surface (A11)
_Thick Dark Surface (Al 2)
—Sandy Mucky Mineral(S1)
—Sandy Gleyed Matrix (S4)
—Sandy Redox (S5)
—Stripped Matrix (S6)
Dark Surface (S7)
Restrictive Layer (if observed):
Type:
Depth (inches):
Remarks:
_ Polyvalue Below Surface (S8) (MLRA 147, 148)
_Thin Dark Surface (S9) (MLRA 147, 148)
_Loamy Mucky Mineral (F1) (MLRA 136)
_ Loamy Gleyed Matrix (F2)
_ Depleted Matrix (F3)
_ Redox Dark Surface (F6)
_ Depleted Dark Surface (F7)
_ Redox Depressions (F8)
_ Iron -Manganese Masses (F12) (LRR N,
MLRA 136)
_ Umbric Surface (F13) (MLRA 122, 136)
—Piedmont Floodplain Soils (F19) (MLRA 148)
Red Parent Material (F21) (MLRA 127, 147, 148)
2Location: PL=Pore Lining, M=Matrix
Indicators for Problematic Hydric Soi
2 cm Muck (A10) (MLRA 147)
—Coast Prairie Redox (A16)
(MLRA 147, 148)
—Piedmont Floodplain Soils (F19)
(MLRA 136, 147)
—Red Parent Material (F21)
(outside MLRA 127, 147, 148)
_Very Shallow Dark Surface (F22)
Other (Explain in Remarks)
3Indicators of hydrophytic vegetation and
wetland hydrology must be present,
unless disturbed or problematic.
Hydric Soil Present? Yes No X
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

U.S. Army Corps of Engineers OMB Control #: 0710-0024, Exp:1113012024
WETLAND DETERMINATION DATA SHEET— Eastern Mountains and Piedmont Region Requirement Control Symbol EXEMPT:
See ERDC/EL TR-12-9; the proponent agency is CECW-CO-R (Authority: AR 335-15, paragraph 5-2a)
Project/Site: Discovery Lake City/County: Durham Sampling Date: 11/02/22
Applicant/Owner: National Institutes of Health State: NC Sampling Point: UPL #2
Investigator(s): J. Farrell - AMT Section, Township, Range:
Landform (hillside, terrace, etc.): back face of dam Local relief (concave, convex, none): concave Slope (%): 20
Subregion (LRR or MLRA): LRR P, MLRA 136 Lat: 35.885752 Long:-78.875504 Datum: NAD 83
Soil Map Unit Name: White Store sandy loam NWI classification: UPL
Are climatic / hydrologic conditions on the site typical for this time of year? Yes X No (If no, explain in Remarks.)
Are Vegetation Soil or Hydrology significantly disturbed? Are "Normal Circumstances" present? Yes X No
Are Vegetation Soil or Hydrology naturally problematic? (If needed, explain any answers in Remarks.)
SUMMARY OF FINDINGS — Attach site map showing sampling point locations, transects, important features, etc.
Hydrophytic Vegetation Present? Yes No X Is the Sampled Area
Hydric Soil Present? Yes No X within a Wetland? Yes No X
Wetland Hydrology Present? Yes No X
Remarks:
HYDROLOGY
Wetland Hydrology Indicators:
Secondary Indicators (minimum of two required)
Primary Indicators (minimum of one is required: check all that apply)
—Surface Soil Cracks (136)
—Surface Water (Al) —True
Aquatic Plants (1314)
—Sparsely Vegetated Concave Surface (138)
_ High Water Table (A2) —Hydrogen
Sulfide Odor (Cl)
_ Drainage Patterns (1310)
—Saturation (A3) —Oxidized
Rhizospheres on Living Roots (C3)
—Moss Trim Lines (1316)
—Water Marks (131) —Presence
of Reduced Iron (C4)
_ Dry -Season Water Table (C2)
_Sediment Deposits (132) _Recent
Iron Reduction in Tilled Soils (C6)
_Crayfish Burrows (C8)
_ Drift Deposits (133) _Thin
Muck Surface (C7)
—Saturation Visible on Aerial Imagery (C9)
_Algal Mat or Crust (134) _Other
(Explain in Remarks)
_Stunted or Stressed Plants (D1)
—Iron Deposits (135)
_ Geomorphic Position (D2)
_ Inundation Visible on Aerial Imagery (137)
—Shallow Aquitard (D3)
—Water-Stained Leaves (139)
_ Microtopographic Relief (D4)
Aquatic Fauna (1313)
FAC-Neutral Test (D5)
Field Observations:
Surface Water Present? Yes No
X Depth (inches):
Water Table Present? Yes No
X Depth (inches):
Saturation Present? Yes No
X Depth (inches):
Wetland
Hydrology Present? Yes No X
(includes capillary fringe)
Describe Recorded Data (stream gauge, monitoring well, aerial photos, previous inspections), if available:
Remarks:
Sample location Is the face of the dam
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

VEGETATION (Four Strata) - Use scientific names of plants.
Sampling Point: UPL #2
Absolute Uominant Indicator
Tree Stratum
(Plot size: )
% Cover Species? Status
Dominance Test worksheet:
1
Number of Dominant Species
2.
That Are OBL, FACW, or FAC: 0 (A)
3.
Total Number of Dominant
4.
Species Across All Strata: 1 (B)
5.
Percent of Dominant Species
6.
That Are OBL, FACW, or FAC: 0.0% (A/B)
7.
Prevalence Index worksheet:
=Total Cover
Total % Cover of: Multiply by:
50% of total cover:
20% of total cover:
OBL species 0 x 1 = 0
Sapling/Shrub Stratum (Plot size:
)
FACW species 0 x 2 = 0
1.
FAC species 0 x 3 = 0
2.
FACU species 0 x 4 = 0
3.
UPL species 0 x 5 = 0
4.
Column Totals: 0 (A) 0 (B)
5.
Prevalence Index = B/A =
6.
Hydrophytic Vegetation Indicators:
7.
_ 1 - Rapid Test for Hydrophytic Vegetation
8.
2 - Dominance Test is >50%
9.
3 - Prevalence Index is !-3.01
=Total Cover
4 - Morphological Adaptations' (Provide supporting
50% of total cover:
20% of total cover:
data in Remarks or on a separate sheet)
Herb Stratum
(Plot size: 40'x40' )
_ Problematic Hydrophytic Vegetation' (Explain)
1. fescue
100 Yes
Indicators of hydric soil and wetland hydrology must
2.
be present, unless disturbed or problematic.
Definitions of Four Vegetation Strata:
3.
4.
Tree - Woody plants, excluding vines, 3 in. (7.6 cm) or
5.
more in diameter at breast height (DBH), regardless of
6
height.
7.
Sapling/Shrub - Woody plants, excluding vines, less
8.
than 3 in. DBH and greater than or equal to 3.28 ft
9
(1 m) tall.
10.
Herb - All herbaceous (non -woody) plants, regardless
11.
of size, and woody plants less than 3.28 ft tall.
100 =Total Cover
Woody Vine - All woody vines greater than 3.28 ft in
50% of total cover:
50 20% of total cover: 20
height.
Woody Vine Stratum (Plot size: )
1.
2.
3.
4.
5
Hydrophytic
=Total Cover
Vegetation
50% of total cover:
20% of total cover:
Present? Yes No X
Remarks: (Include photo numbers here or on a separate sheet.)
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont - Version 2.0

SOIL Sampling Point: UPL #2
Profile Description: (Describe to the depth needed to document the indicator or confirm the absence of indicators.)
Depth Matrix Redox Features
(inches) Color (moist) % Color (moist) % Type LoC2 Texture Remarks
0-12 5YR 5/4 100 Loamy/Clayey dam fill
Type: C=Concentration, D=Depletion, RM=Reduced Matrix, MS=Masked Sand Grains.
Hydric Soil Indicators:
_ Histosol (Al)
_ Histic Epipedon (A2)
_ Black Histic (A3)
—Hydrogen Sulfide (A4)
—Stratified Layers (A5)
2 cm Muck (A10) (LRR N)
_ Depleted Below Dark Surface (A11)
_Thick Dark Surface (Al 2)
—Sandy Mucky Mineral(S1)
—Sandy Gleyed Matrix (S4)
—Sandy Redox (S5)
—Stripped Matrix (S6)
Dark Surface (S7)
Restrictive Layer (if observed):
Type:
Depth (inches):
Remarks:
_ Polyvalue Below Surface (S8) (MLRA 147, 148)
_Thin Dark Surface (S9) (MLRA 147, 148)
_Loamy Mucky Mineral (F1) (MLRA 136)
_ Loamy Gleyed Matrix (F2)
_ Depleted Matrix (F3)
_ Redox Dark Surface (F6)
_ Depleted Dark Surface (F7)
_ Redox Depressions (F8)
_ Iron -Manganese Masses (F12) (LRR N,
MLRA 136)
_ Umbric Surface (F13) (MLRA 122, 136)
—Piedmont Floodplain Soils (F19) (MLRA 148)
Red Parent Material (F21) (MLRA 127, 147, 148)
2Location: PL=Pore Lining, M=Matrix
Indicators for Problematic Hydric Soi
2 cm Muck (A10) (MLRA 147)
—Coast Prairie Redox (A16)
(MLRA 147, 148)
Piedmont Floodplain Soils (F19)
(MLRA 136, 147)
—Red Parent Material (F21)
(outside MLRA 127, 147, 148)
_Very Shallow Dark Surface (F22)
Other (Explain in Remarks)
3Indicators of hydrophytic vegetation and
wetland hydrology must be present,
unless disturbed or problematic.
Hydric Soil Present? Yes No X
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

U.S. Army Corps of Engineers OMB Control #: 0710-0024, Exp:1113012024
WETLAND DETERMINATION DATA SHEET— Eastern Mountains and Piedmont Region Requirement Control Symbol EXEMPT:
See ERDC/EL TR-12-9; the proponent agency is CECW-CO-R (Authority: AR 335-15, paragraph 5-2a)
Project/Site: Discovery Lake City/County: Durham Sampling Date: 11/02/22
Applicant/Owner: National Institutes of Health State: NC Sampling Point: WTL #1
Investigator(s): J. Farrell - AMT Section, Township, Range:
Landform (hillside, terrace, etc.): hillside Local relief (concave, convex, none): concave Slope (%): 3
Subregion (LRR or MLRA): LRR P, MLRA 136 Lat: 35.886592 Long:-78.875725 Datum: NAD 83
Soil Map Unit Name: Chewacla and Wehadkee NWI classification: PFO
Are climatic / hydrologic conditions on the site typical for this time of year? Yes X No (If no, explain in Remarks.)
Are Vegetation Soil or Hydrology significantly disturbed? Are "Normal Circumstances" present? Yes X No
Are Vegetation Soil or Hydrology naturally problematic? (If needed, explain any answers in Remarks.)
SUMMARY OF FINDINGS — Attach site map showing sampling point locations, transects, important features, etc.
Hydrophytic Vegetation Present? Yes X No Is the Sampled Area
Hydric Soil Present? Yes X No within a Wetland? Yes X No
Wetland Hydrology Present? Yes X No
Remarks:
HYDROLOGY
Wetland Hydrology Indicators:
Secondary Indicators (minimum of two required)
Primary Indicators (minimum of one is required: check all that apply)
—Surface Soil Cracks (136)
—Surface Water (Al) —True
Aquatic Plants (1314)
—Sparsely Vegetated Concave Surface (138)
_ High Water Table (A2) —Hydrogen
Sulfide Odor (Cl)
X Drainage Patterns (1310)
—Saturation (A3) —Oxidized
Rhizospheres on Living Roots (C3)
—Moss Trim Lines (1316)
X Water Marks (131) X
Presence of Reduced Iron (C4)
_ Dry -Season Water Table (C2)
X Sediment Deposits (132) _Recent
Iron Reduction in Tilled Soils (C6)
_Crayfish Burrows (C8)
X Drift Deposits (133) _Thin
Muck Surface (C7)
_Saturation Visible on Aerial Imagery (C9)
_Algal Mat or Crust (134) _Other
(Explain in Remarks)
_Stunted or Stressed Plants (D1)
—Iron Deposits (135)
X Geomorphic Position (D2)
_ Inundation Visible on Aerial Imagery (137)
—Shallow Aquitard (D3)
—Water-Stained Leaves (139)
_ Microtopographic Relief (D4)
Aquatic Fauna (1313)
X FAC-Neutral Test (D5)
Field Observations:
Surface Water Present? Yes No
X Depth (inches):
Water Table Present? Yes No
X Depth (inches):
Saturation Present? Yes No
X Depth (inches):
Wetland
Hydrology Present? Yes X No
(includes capillary fringe)
Describe Recorded Data (stream gauge, monitoring well, aerial photos, previous inspections), if available:
Remarks:
Sample location is near the outfall of Discovery Lake with PFO wetland
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

VEGETATION (Four Strata) - Use scientific names of plants.
Sampling Point: WTL #1
Absolute
Uominant
Indicator
Tree Stratum (Plot size: 40'x40' )
% Cover
Species?
Status
Dominance Test worksheet:
1.
Pinus taeda
20
Yes
FAC
Number of Dominant Species
2.
Ulmus rubra
15
Yes
FAC
That Are OBL, FACW, or FAC: 10 (A)
3.
Salix nigra
5
No
OBL
Total Number of Dominant
4.
Platanus occidentalis
15
Yes
FACW
Species Across All Strata: 12 (B)
5.
Ilex opaca
10
No
FACU
Percent of Dominant Species
6.
Liquidambarstyraciflua
15
Yes
FAC
That Are OBL, FACW, or FAC: 83.3% (A/B)
7.
Quercus phellos
15
Yes
FAC
Prevalence Index worksheet:
95
=Total Cover
Total % Cover of: Multiply by:
50% of total cover:
48
20% of total cover:
19
OBL species 5 x 1 = 5
Sapling/Shrub Stratum (Plot size: 40'x40'
)
FACW species 65 x 2 = 130
1.
Acer rubrum
20
Yes
FAC
FAC species 150 x 3 = 450
2.
Fraxinus pennsylvanica
15
No
FACW
FACU species 70 x 4 = 280
3.
Liriodendron tulipifera
20
Yes
FACU
UPL species 0 x 5 = 0
4.
Ulmus rubra
25
Yes
FAC
Column Totals: 290 (A) 865 (B)
5.
Prevalence Index = B/A = 2.98
6.
Hydrophytic Vegetation Indicators:
7.
_ 1 - Rapid Test for Hydrophytic Vegetation
8.
X 2 - Dominance Test is >50%
9.
X 3 - Prevalence Index is !2.01
80
=Total Cover
4 - Morphological Adaptations' (Provide supporting
50% of total cover:
40
20% of total cover:
16
data in Remarks or on a separate sheet)
Herb Stratum (Plot size: 40'x40' )
_ Problematic Hydrophytic Vegetation' (Explain)
1.
Microstegium vimineum
40
Yes
FAC
Indicators of hydric soil and wetland hydrology must
2.
Arundinaria tecta
20
Yes
FACW
be present, unless disturbed or problematic.
Definitions of Four Vegetation Strata:
3.
4.
Tree - Woody plants, excluding vines, 3 in. (7.6 cm) or
5.
more in diameter at breast height (DBH), regardless of
6
height.
7.
Sapling/Shrub - Woody plants, excluding vines, less
8.
than 3 in. DBH and greater than or equal to 3.28 ft
9
(1 m) tall.
10.
Herb - All herbaceous (non -woody) plants, regardless
11.
of size, and woody plants less than 3.28 ft tall.
60
=Total Cover
Woody Vine - All woody vines greater than 3.28 ft in
50% of total cover:
30
20% of total cover:
12
height.
Woody Vine Stratum (Plot size: 40'x40' )
1.
Lonicera japonica
40
Yes
FACU
2.
Vitis cinerea
15
Yes
FACW
3.
4.
5
Hydrophytic
55
=Total Cover
Vegetation
50% of total cover:
28
20% of total cover:
11
Present? Yes X No
Remarks: (Include photo numbers here or on a separate sheet.)
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont - Version 2.0

SOIL Sampling Point: WTL #1
Profile Description: (Describe to the depth needed to document the indicator or confirm the absence of indicators.)
Depth Matrix
Redox Features
(inches) Color (moist) %
Color (moist) % Type LoC2 Texture Remarks
0-10 7.5YR 2.5/2 100
Loamy/Clayey mostly loam
10-18 2.5YR 7/3 70
7.5YR 6/8 30 RM M Mucky Loam/Clay mostly clay
Type: C=Concentration, D=Depletion, RM=Reduced Matrix, MS=Masked Sand Grains.
2Location: PL=Pore Lining, M=Matrix.
Hydric Soil Indicators:
Indicators for Problematic Hydric Soils3:
_ Histosol (Al)
_ Polyvalue Below Surface (S8) (MLRA 147, 148)
_ 2 cm Muck (A10) (MLRA 147)
Histic Epipedon (A2)
_Thin Dark Surface (S9) (MLRA 147, 148)
_Coast Prairie Redox (A16)
_ Black Histic (A3)
X Loamy Mucky Mineral (F1) (MLRA 136)
(MLRA 147, 148)
—Hydrogen Sulfide (A4)
_ Loamy Gleyed Matrix (F2)
X Piedmont Floodplain Soils (F19)
—Stratified Layers (A5)
_ Depleted Matrix (F3)
(MLRA 136, 147)
_ 2 cm Muck (A10) (LRR N)
_ Redox Dark Surface (F6)
—Red Parent Material (F21)
_ Depleted Below Dark Surface (A11)
_ Depleted Dark Surface (F7)
(outside MLRA 127, 147, 148)
_Thick Dark Surface (Al 2)
_ Redox Depressions (F8)
_Very Shallow Dark Surface (F22)
_Sandy Mucky Mineral (S1)
X Iron -Manganese Masses (F12) (LRR N,
_Other (Explain in Remarks)
Gleyed Matrix (S4)
MLRA 136)
_Sandy
—Sandy Redox (S5)
_ Umbric Surface (F13) (MLRA 122, 136)
3Indicators of hydrophytic vegetation and
—Stripped Matrix (S6)
—Piedmont Floodplain Soils (F19) (MLRA 148)
wetland hydrology must be present,
Dark Surface (S7)
Red Parent Material (F21) (MLRA 127, 147, 148)
unless disturbed or problematic.
Restrictive Layer (if observed):
Type:
Depth (inches):
Hydric Soil Present? Yes X No
Remarks:
The presence of a reduced matrix within 12" of the soil surface indicates that this soil is hydric based on the hydric soil definition: "a soil that formed
under conditions of saturation, flooding or ponding long enough during the growing season to develop anaerobic conditions in the upper part'.
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

U.S. Army Corps of Engineers OMB Control #: 0710-0024, Exp:1113012024
WETLAND DETERMINATION DATA SHEET— Eastern Mountains and Piedmont Region Requirement Control Symbol EXEMPT:
See ERDC/EL TR-12-9; the proponent agency is CECW-CO-R (Authority: AR 335-15, paragraph 5-2a)
Project/Site: Discovery Lake City/County: Durham Sampling Date: 11/02/22
Applicant/Owner: National Institutes of Health State: NC Sampling Point: WTL #2
Investigator(s): J. Farrell - AMT Section, Township, Range:
Landform (hillside, terrace, etc.): back face of dam Local relief (concave, convex, none): concave Slope (%): 5
Subregion (LRR or MLRA): LRR P, MLRA 136 Lat: 35.885708 Long:-78.875504 Datum: NAD 83
Soil Map Unit Name: White Store sandy loam NWI classification: LEM
Are climatic / hydrologic conditions on the site typical for this time of year? Yes X No (If no, explain in Remarks.)
Are Vegetation Soil or Hydrology significantly disturbed? Are "Normal Circumstances" present? Yes X No
Are Vegetation Soil or Hydrology naturally problematic? (If needed, explain any answers in Remarks.)
SUMMARY OF FINDINGS — Attach site map showing sampling point locations, transects, important features, etc.
Hydrophytic Vegetation Present? Yes X No Is the Sampled Area
Hydric Soil Present? Yes X No within a Wetland? Yes X No
Wetland Hydrology Present? Yes X No
Remarks:
HYDROLOGY
Wetland Hydrology Indicators:
Secondary Indicators (minimum of two required)
Primary Indicators (minimum of one is required: check all that apply)
—Surface Soil Cracks (136)
X Surface Water (Al) _True
Aquatic Plants (1314)
_Sparsely Vegetated Concave Surface (138)
X High Water Table (A2) —Hydrogen
Sulfide Odor (Cl)
_ Drainage Patterns (1310)
X Saturation (A3) —Oxidized
Rhizospheres on Living Roots (C3)
—Moss Trim Lines (1316)
—Water Marks (131) —Presence
of Reduced Iron (C4)
_ Dry -Season Water Table (C2)
_Sediment Deposits (132) _Recent
Iron Reduction in Tilled Soils (C6)
_Crayfish Burrows (C8)
_ Drift Deposits (133) _Thin
Muck Surface (C7)
_Saturation Visible on Aerial Imagery (C9)
_Algal Mat or Crust (134) _Other
(Explain in Remarks)
_Stunted or Stressed Plants (D1)
—Iron Deposits (135)
_ Geomorphic Position (D2)
_ Inundation Visible on Aerial Imagery (137)
—Shallow Aquitard (D3)
—Water-Stained Leaves (139)
_ Microtopographic Relief (D4)
Aquatic Fauna (1313)
X FAC-Neutral Test (D5)
Field Observations:
Surface Water Present? Yes X No
Depth (inches): 24
Water Table Present? Yes X No
Depth (inches): 3
Saturation Present? Yes X No
Depth (inches): 2
Wetland
Hydrology Present? Yes X No
(includes capillary fringe)
Describe Recorded Data (stream gauge, monitoring well, aerial photos, previous inspections), if available:
Remarks:
Sample location Is the edge of lake and base of dam
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

VEGETATION (Four Strata) - Use scientific names of plants.
Sampling Point: WTL #2
Absolute
Uominant
Indicator
Tree Stratum (Plot size: )
% Cover
Species?
Status
Dominance Test worksheet:
1
Number of Dominant Species
2.
That Are OBL, FACW, or FAC: 6 (A)
3.
Total Number of Dominant
4.
Species Across All Strata: 6 (B)
5.
Percent of Dominant Species
6.
That Are OBL, FACW, or FAC: 100.0% (A/B)
7.
Prevalence Index worksheet:
=Total Cover
Total % Cover of: Multiply by:
50% of total cover:
20% of total cover:
OBL species 65 x 1 = 65
Sapling/Shrub Stratum (Plot size: 3X20'
)
FACW species 40 x 2 = 80
1.
Salix nigra
15
Yes
OBL
FAC species 35 x 3 = 105
2.
Liquidambar styraciflua
15
Yes
FAC
FACU species 10 x 4 = 40
3.
Iva frutescens
20
Yes
FACW
UPL species 0 x 5 = 0
4.
Hibiscus moscheutos
10
No
OBL
Column Totals: 150 (A) 290 (B)
5.
Alnus serrulata
10
No
OBL
Prevalence Index = B/A = 1.93
6.
Hydrophytic Vegetation Indicators:
7.
_ 1 - Rapid Test for Hydrophytic Vegetation
8.
X 2 - Dominance Test is >50%
9.
X 3 - Prevalence Index is !2.01
70
=Total Cover
4 - Morphological Adaptations' (Provide supporting
50% of total cover:
35 20% of total cover:
14
data in Remarks or on a separate sheet)
Herb Stratum (Plot size: 40'x40' )
_ Problematic Hydrophytic Vegetation' (Explain)
1.
fescue
10
No
FACU
Indicators of hydric soil and wetland hydrology must
2.
Microstegium vimineum
20
Yes
FAC
be present, unless disturbed or problematic.
Definitions of Four Vegetation Strata:
3. Juncus effusus 20 Yes FACW
4.
Solidago patula
30
Yes
OBL
Tree - Woody plants, excluding vines, 3 in. (7.6 cm) or
5.
more in diameter at breast height (DBH), regardless of
6
height.
7.
Sapling/Shrub - Woody plants, excluding vines, less
8.
than 3 in. DBH and greater than or equal to 3.28 ft
9
(1 m) tall.
10.
Herb - All herbaceous (non -woody) plants, regardless
11.
of size, and woody plants less than 3.28 ft tall.
80
=Total Cover
Woody Vine - All woody vines greater than 3.28 ft in
50% of total cover:
40 20% of total cover:
16
height.
Woody Vine Stratum (Plot size: )
1.
2.
3.
4.
5
Hydrophytic
=Total Cover
Vegetation
50% of total cover:
20% of total cover:
Present? Yes X No
Remarks: (Include photo numbers here or on a separate sheet.)
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont - Version 2.0

SOIL Sampling Point: WTL #2
Profile Description: (Describe to the depth needed to document the indicator or confirm the absence of indicators.)
Depth Matrix
Redox Features
(inches) Color (moist) %
Color (moist) % Type LoC2 Texture Remarks
0-10 5YR 4/3 100
Loamy/Clayey dam fill
10-16 7.5YR 8/3 100
Loamy/Clayey
Type: C=Concentration, D=Depletion, RM=Reduced Matrix, MS=Masked Sand Grains.
2Location: PL=Pore Lining, M=Matrix.
Hydric Soil Indicators:
Indicators for Problematic Hydric Soils3:
_ Histosol (Al)
_ Polyvalue Below Surface (S8) (MLRA 147, 148)
_ 2 cm Muck (A10) (MLRA 147)
Histic Epipedon (A2)
_Thin Dark Surface (S9) (MLRA 147, 148)
_Coast Prairie Redox (A16)
_ Black Histic (A3)
X Loamy Mucky Mineral (F1) (MLRA 136)
(MLRA 147, 148)
—Hydrogen Sulfide (A4)
_ Loamy Gleyed Matrix (F2)
X Piedmont Floodplain Soils (F19)
—Stratified Layers (A5)
_ Depleted Matrix (F3)
(MLRA 136, 147)
_ 2 cm Muck (A10) (LRR N)
_ Redox Dark Surface (F6)
—Red Parent Material (F21)
_ Depleted Below Dark Surface (A11)
_ Depleted Dark Surface (F7)
(outside MLRA 127, 147, 148)
_Thick Dark Surface (Al 2)
_ Redox Depressions (F8)
_Very Shallow Dark Surface (F22)
—Sandy Mucky Mineral (S1)
_ Iron -Manganese Masses (F12) (LRR N,
—Other (Explain in Remarks)
Gleyed Matrix (S4)
MLRA 136)
_Sandy
—Sandy Redox (S5)
_ Umbric Surface (F13) (MLRA 122, 136)
3Indicators of hydrophytic vegetation and
—Stripped Matrix (S6)
—Piedmont Floodplain Soils (F19) (MLRA 148)
wetland hydrology must be present,
Dark Surface (S7)
Red Parent Material (F21) (MLRA 127, 147, 148)
unless disturbed or problematic.
Restrictive Layer (if observed):
Type:
Depth (inches):
Hydric Soil Present? Yes X No
Remarks:
Soil parameters are skewed due to dam fill material
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

U.S. Army Corps of Engineers OMB Control #: 0710-0024, Exp:1113012024
WETLAND DETERMINATION DATA SHEET— Eastern Mountains and Piedmont Region Requirement Control Symbol EXEMPT:
See ERDC/EL TR-12-9; the proponent agency is CECW-CO-R (Authority: AR 335-15, paragraph 5-2a)
Project/Site: Discovery Lake City/County: Durham Sampling Date: 11/02/22
Applicant/Owner: National Institutes of Health State: NC Sampling Point: WTL #3
Investigator(s): J. Farrell - AMT Section, Township, Range:
Landform (hillside, terrace, etc.): edge of lake Local relief (concave, convex, none): concave Slope (%): 1
Subregion (LRR or MLRA): LRR P, MLRA 136 Lat: 35.882272 Long:-78.875563 Datum: NAD 83
Soil Map Unit Name: White Store sandy loam NWI classification: LEM/LSS
Are climatic / hydrologic conditions on the site typical for this time of year? Yes X No (If no, explain in Remarks.)
Are Vegetation Soil or Hydrology significantly disturbed? Are "Normal Circumstances" present? Yes X No
Are Vegetation Soil or Hydrology naturally problematic? (If needed, explain any answers in Remarks.)
SUMMARY OF FINDINGS — Attach site map showing sampling point locations, transects, important features, etc.
Hydrophytic Vegetation Present? Yes X No Is the Sampled Area
Hydric Soil Present? Yes X No within a Wetland? Yes X No
Wetland Hydrology Present? Yes X No
Remarks:
HYDROLOGY
Wetland Hydrology Indicators:
Secondary Indicators (minimum of two required)
Primary Indicators (minimum of one is required: check all that apply)
—Surface Soil Cracks (136)
X Surface Water (Al)
X True Aquatic Plants (1314)
—Sparsely Vegetated Concave Surface (138)
X High Water Table (A2)
—Hydrogen Sulfide Odor (Cl)
_ Drainage Patterns (1310)
X Saturation (A3)
—Oxidized Rhizospheres on Living Roots (C3)
—Moss Trim Lines (1316)
—Water Marks (131)
—Presence of Reduced Iron (C4)
_ Dry -Season Water Table (C2)
_Sediment Deposits (132)
_Recent Iron Reduction in Tilled Soils (C6)
X Crayfish Burrows (C8)
_ Drift Deposits (133)
_Thin Muck Surface (C7)
_Saturation Visible on Aerial Imagery (C9)
_Algal Mat or Crust (134)
_Other (Explain in Remarks)
_Stunted or Stressed Plants (D1)
—Iron Deposits (135)
_ Geomorphic Position (D2)
_ Inundation Visible on Aerial Imagery (137)
—Shallow Aquitard (D3)
—Water-Stained Leaves (139)
_ Microtopographic Relief (D4)
X Aquatic Fauna (1313)
X FAC-Neutral Test (D5)
Field Observations:
Surface Water Present? Yes X
No Depth (inches): 24
Water Table Present? Yes X
No Depth (inches): 3
Saturation Present? Yes X
No Depth (inches): 1
Wetland
Hydrology Present? Yes X No
(includes capillary fringe)
Describe Recorded Data (stream gauge, monitoring well, aerial photos, previous inspections), if available:
Remarks:
Sample location Is at the edge of the lake
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

VEGETATION (Four Strata) - Use scientific names of plants.
Sampling Point: WTL #3
Absolute
Uominant
Indicator
Tree Stratum (Plot size: )
% Cover
Species?
Status
Dominance Test worksheet:
1
Number of Dominant Species
2.
That Are OBL, FACW, or FAC: 7 (A)
3.
Total Number of Dominant
4.
Species Across All Strata: 7 (B)
5.
Percent of Dominant Species
6.
That Are OBL, FACW, or FAC: 100.0% (A/B)
7.
Prevalence Index worksheet:
=Total Cover
Total % Cover of: Multiply by:
50% of total cover:
20% of total cover:
OBL species 85 x 1 = 85
Sapling/Shrub Stratum (Plot size: 3X20'
)
FACW species 30 x 2 = 60
1.
Salix nigra
15
Yes
OBL
FAC species 35 x 3 = 105
2.
Liquidambar styraciflua
15
Yes
FAC
FACU species 10 x 4 = 40
3.
Iva frutescens
15
Yes
FACW
UPL species 0 x 5 = 0
4.
Alnus serrulata
15
Yes
OBL
Column Totals: 160 (A) 290 (B)
5.
Prevalence Index = B/A = 1.81
6.
Hydrophytic Vegetation Indicators:
7.
_ 1 - Rapid Test for Hydrophytic Vegetation
8.
X 2 - Dominance Test is >50%
9.
X 3 - Prevalence Index is !2.01
60
=Total Cover
4 - Morphological Adaptations' (Provide supporting
50% of total cover:
30 20% of total cover:
12
data in Remarks or on a separate sheet)
Herb Stratum (Plot size: 3'X20' )
_ Problematic Hydrophytic Vegetation' (Explain)
1.
fescue
10
No
FACU
Indicators of hydric soil and wetland hydrology must
2.
Microstegium vimineum
20
Yes
FAC
be present, unless disturbed or problematic.
Definitions of Four Vegetation Strata:
3. Juncus effusus 15 No FACW
4.
Solidago patula
20
Yes
OBL
Tree - Woody plants, excluding vines, 3 in. (7.6 cm) or
5.
Schoenoplectus pungens
20
Yes
OBL
more in diameter at breast height (DBH), regardless of
6.
Carex lurida
15
No
OBL
height.
7.
Sapling/Shrub - Woody plants, excluding vines, less
8.
than 3 in. DBH and greater than or equal to 3.28 ft
9
(1 m) tall.
10.
Herb - All herbaceous (non -woody) plants, regardless
11.
of size, and woody plants less than 3.28 ft tall.
100
=Total Cover
Woody Vine - All woody vines greater than 3.28 ft in
50% of total cover:
50 20% of total cover:
20
height.
Woody Vine Stratum (Plot size: )
1.
2.
3.
4.
5
Hydrophytic
=Total Cover
Vegetation
50% of total cover:
20% of total cover:
Present? Yes X No
Remarks: (Include photo numbers here or on a separate sheet.)
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont - Version 2.0

SOIL Sampling Point: WTL #3
Profile Description: (Describe to the depth needed to document the indicator or confirm the absence of indicators.)
Depth Matrix Redox Features
(inches) Color (moist) % Color (moist) % Type LoC2 Texture Remarks
0-8 7.5YR 2.5/3 100 Loamy/Clayey
8-14 7.5YR 8/2 75 7.5YR 6/6 25 MS PL/M Loamy/Clayey
Type: C=Concentration, D=Depletion, RM=Reduced Matrix, MS=Masked Sand Grains.
Hydric Soil Indicators:
_ Histosol (Al)
_ Histic Epipedon (A2)
_ Black Histic (A3)
—Hydrogen Sulfide (A4)
—Stratified Layers (A5)
2 cm Muck (A10) (LRR N)
_ Depleted Below Dark Surface (A11)
_Thick Dark Surface (Al 2)
—Sandy Mucky Mineral(S1)
—Sandy Gleyed Matrix (S4)
—Sandy Redox (S5)
—Stripped Matrix (S6)
Dark Surface (S7)
Restrictive Layer (if observed):
Type:
Depth (inches):
Remarks:
_ Polyvalue Below Surface (S8) (MLRA 147, 148)
_Thin Dark Surface (S9) (MLRA 147, 148)
X Loamy Mucky Mineral (F1) (MLRA 136)
_ Loamy Gleyed Matrix (F2)
_ Depleted Matrix (F3)
_ Redox Dark Surface (F6)
_ Depleted Dark Surface (F7)
_ Redox Depressions (F8)
_ Iron -Manganese Masses (F12) (LRR N,
MLRA 136)
_ Umbric Surface (F13) (MLRA 122, 136)
—Piedmont Floodplain Soils (F19) (MLRA 148)
Red Parent Material (F21) (MLRA 127, 147, 148)
2Location: PL=Pore Lining, M=Matrix
Indicators for Problematic Hydric Soil
2 cm Muck (A10) (MLRA 147)
—Coast Prairie Redox (A16)
(MLRA 147, 148)
X Piedmont Floodplain Soils (F19)
(MLRA 136, 147)
—Red Parent Material (F21)
(outside MLRA 127, 147, 148)
_Very Shallow Dark Surface (F22)
Other (Explain in Remarks)
3Indicators of hydrophytic vegetation and
wetland hydrology must be present,
unless disturbed or problematic.
Hydric Soil Present? Yes X No
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

U.S. Army Corps of Engineers OMB Control #: 0710-0024, Exp:1113012024
WETLAND DETERMINATION DATA SHEET— Eastern Mountains and Piedmont Region Requirement Control Symbol EXEMPT:
See ERDC/EL TR-12-9; the proponent agency is CECW-CO-R (Authority: AR 335-15, paragraph 5-2a)
Project/Site: Discovery Lake City/County: Durham Sampling Date: 11/02/22
Applicant/Owner: National Institutes of Health State: NC Sampling Point: WTL #4
Investigator(s): J. Farrell - AMT Section, Township, Range:
Landform (hillside, terrace, etc.): edge of lake Local relief (concave, convex, none): concave Slope (%): 1
Subregion (LRR or MLRA): LRR P, MLRA 136 Lat: 35.882272 Long:-78.875563 Datum: NAD 83
Soil Map Unit Name: White Store sandy loam NWI classification: LEM
Are climatic / hydrologic conditions on the site typical for this time of year? Yes X No (If no, explain in Remarks.)
Are Vegetation Soil or Hydrology significantly disturbed? Are "Normal Circumstances" present? Yes X No
Are Vegetation Soil or Hydrology naturally problematic? (If needed, explain any answers in Remarks.)
SUMMARY OF FINDINGS — Attach site map showing sampling point locations, transects, important features, etc.
Hydrophytic Vegetation Present? Yes X No Is the Sampled Area
Hydric Soil Present? Yes X No within a Wetland? Yes X No
Wetland Hydrology Present? Yes X No
Remarks:
HYDROLOGY
Wetland Hydrology Indicators:
Secondary Indicators (minimum of two required)
Primary Indicators (minimum of one is required: check all that apply)
—Surface Soil Cracks (136)
X Surface Water (Al)
X True Aquatic Plants (1314)
—Sparsely Vegetated Concave Surface (138)
X High Water Table (A2)
—Hydrogen Sulfide Odor (Cl)
_ Drainage Patterns (1310)
X Saturation (A3)
—Oxidized Rhizospheres on Living Roots (C3)
—Moss Trim Lines (1316)
—Water Marks (131)
—Presence of Reduced Iron (C4)
_ Dry -Season Water Table (C2)
_Sediment Deposits (132)
_Recent Iron Reduction in Tilled Soils (C6)
X Crayfish Burrows (C8)
_ Drift Deposits (133)
_Thin Muck Surface (C7)
—Saturation Visible on Aerial Imagery (C9)
_Algal Mat or Crust (134)
_Other (Explain in Remarks)
_Stunted or Stressed Plants (D1)
—Iron Deposits (135)
_ Geomorphic Position (D2)
_ Inundation Visible on Aerial Imagery (137)
—Shallow Aquitard (D3)
—Water-Stained Leaves (139)
_ Microtopographic Relief (D4)
X Aquatic Fauna (1313)
X FAC-Neutral Test (D5)
Field Observations:
Surface Water Present? Yes X
No Depth (inches): 24
Water Table Present? Yes X
No Depth (inches): 3
Saturation Present? Yes X
No Depth (inches): 1
Wetland
Hydrology Present? Yes X No
(includes capillary fringe)
Describe Recorded Data (stream gauge, monitoring well, aerial photos, previous inspections), if available:
Remarks:
Sample location Is at the edge of the lake
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0

VEGETATION (Four Strata) - Use scientific names of plants.
Sampling Point: WTL #4
Absolute Uominant Indicator
Tree Stratum (Plot size: ) % Cover Species? Status
2.
3.
4.
5.
6.
7.
50% of total cover:
Sapling/Shrub Stratum (Plot size: 3X20' )
1. Salix nigra
2. Liquidambar styraciflua
3. Iva frutescens
4. Alnus serrulata
5.
6.
7.
8.
9.
50% of total cover: 30
Herb Stratum (Plot size: 3X20' )
1. fescue
2. Microstegium vimineum
3. Juncus effusus
4. Solidago patula
5. Schoenoplectus pungens
6. Carex lurida
7.
8.
9.
10.
11.
50% of total cover: 50
Woody Vine Stratum (Plot size: )
1.
2.
3.
4.
5.
_=Total Cover
20% of total cover
15 Yes
OBL
15 Yes
FAC
15 Yes
FACW
15 Yes
OBL
60 =Total Cover
20% of total cover:
12
10 No
FACU
20 Yes
FAC
15 No
FACW
20 Yes
OBL
20 Yes
OBL
15 No
OBL
100 =Total Cover
20% of total cover: 20
=Total Cover
50% of total cover: 20% of total cover:
Remarks: (Include photo numbers here or on a separate sheet.)
Dominance Test worksheet:
Number of Dominant Species
That Are OBL, FACW, or FAC: 7 (A)
Total Number of Dominant
Species Across All Strata: 7 (B)
Percent of Dominant Species
That Are OBL, FACW, or FAC: 100.0% (A/B)
Prevalence Index worksheet:
Total % Cover of: Multiply by:
OBL species 85 x 1 = 85
FACW species 30 x 2 = 60
FAC species 35 x 3 = 105
FACU species 10 x 4 = 40
UPL species 0 x 5 = 0
Column Totals: 160 (A) 290 (B)
Prevalence Index = B/A = 1.81
Hydrophytic Vegetation Indicators:
_ 1 - Rapid Test for Hydrophytic Vegetation
X 2 - Dominance Test is >50%
X 3 - Prevalence Index is !-3.01
4 - Morphological Adaptations' (Provide supporting
data in Remarks or on a separate sheet)
-Problematic Hydrophytic Vegetation' (Explain)
Indicators of hydric soil and wetland hydrology must
be present, unless disturbed or problematic.
Definitions of Four Vegetation Strata:
Tree - Woody plants, excluding vines, 3 in. (7.6 cm) or
more in diameter at breast height (DBH), regardless of
height.
Sapling/Shrub - Woody plants, excluding vines, less
than 3 in. DBH and greater than or equal to 3.28 ft
(1 m) tall.
Herb - All herbaceous (non -woody) plants, regardless
of size, and woody plants less than 3.28 ft tall.
Woody Vine - All woody vines greater than 3.28 ft in
height.
Hydrophytic
Vegetation
Present? Yes X No
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont - Version 2.0

SOIL Sampling Point: WTL #4
Profile Description: (Describe to the depth needed to document the indicator or confirm the absence of indicators.)
Depth Matrix Redox Features
(inches) Color (moist) % Color (moist) % Type LoC2 Texture Remarks
0-8 7.5YR 2.5/3 100 Loamy/Clayey
8-14 7.5YR 8/2 75 7.5YR 6/6 25 MS PL/M Loamy/Clayey
Type: C=Concentration, D=Depletion, RM=Reduced Matrix, MS=Masked Sand Grains.
Hydric Soil Indicators:
_ Histosol (Al)
_ Histic Epipedon (A2)
_ Black Histic (A3)
—Hydrogen Sulfide (A4)
—Stratified Layers (A5)
2 cm Muck (A10) (LRR N)
_ Depleted Below Dark Surface (A11)
_Thick Dark Surface (Al 2)
—Sandy Mucky Mineral(S1)
—Sandy Gleyed Matrix (S4)
—Sandy Redox (S5)
—Stripped Matrix (S6)
Dark Surface (S7)
Restrictive Layer (if observed):
Type:
Depth (inches):
Remarks:
_ Polyvalue Below Surface (S8) (MLRA 147, 148)
_Thin Dark Surface (S9) (MLRA 147, 148)
X Loamy Mucky Mineral (F1) (MLRA 136)
_ Loamy Gleyed Matrix (F2)
_ Depleted Matrix (F3)
_ Redox Dark Surface (F6)
_ Depleted Dark Surface (F7)
_ Redox Depressions (F8)
_ Iron -Manganese Masses (F12) (LRR N,
MLRA 136)
_ Umbric Surface (F13) (MLRA 122, 136)
—Piedmont Floodplain Soils (F19) (MLRA 148)
Red Parent Material (F21) (MLRA 127, 147, 148)
2Location: PL=Pore Lining, M=Matrix
Indicators for Problematic Hydric Soil
2 cm Muck (A10) (MLRA 147)
—Coast Prairie Redox (A16)
(MLRA 147, 148)
X Piedmont Floodplain Soils (F19)
(MLRA 136, 147)
—Red Parent Material (F21)
(outside MLRA 127, 147, 148)
_Very Shallow Dark Surface (F22)
Other (Explain in Remarks)
3Indicators of hydrophytic vegetation and
wetland hydrology must be present,
unless disturbed or problematic.
Hydric Soil Present? Yes X No
ENG FORM 6116-4, JUL 2018 Eastern Mountains and Piedmont — Version 2.0