NC0047597_More Information Received_20160121

F ,I■Gu
V P�M� �► �� N�00 V7577 Administration
919-560-4381
1869
CITY OF MEDICINE
CITY OF DURHAM
DEPARTMENT OF WATER MANAGEMENT
101 CITY HALL PLAZA • DURHAM, NC 27701
919-560-4381•FAX [phone]
www.durhamnc.gov
RECEIVED/NCDEQ/DWR
January 21, 2016
NC DWR/NPDES Complex Permitting Unit
FEB -1 2016
Water quality
Permitting Section
Customer Billing Services
919-560-4412
Laboratory Services
919-560-4386
Plant Maintenance
919-560-4388
Regulatory Compliance
919-560-4381
Utility Engineering
919-560-4381
Wastewater Treatment
North - 919-560-4384
South - 919-560-4386
Water Supply & Treatment
Williams - 919-560-4348
Brown - 919-560-4362
Water & Sewer Maintenance
919-560-4344
Attn: Tom Belnick� t
1617 Mail Service Center ELyr f e we"
Raleigh, North Carolina 27699-1617
Zn qS
�SUBJECT: November / December 2015 Toxicity Testing fUrrelirMcI f>!nc
South Durham Water Reclamation Facility, NCO047597
Please find enclosed three (3) copies of the November and December 2015
toxicity results for the South Durham Water Reclamation Facility,
NC0047597. They are to be inserted into each of the three Permit Renewal
Packets that were delivered to your office on October 30, 2015.
Please note that these tests were the Fat -head Minnow species only and they
were required as part of our Permit Renewal Application. The analyses were
completed by ETT Environmental Inc., 4 Craftsman Court, Greer, SC. through
Pace Analytical Services Inc., 6701 Conference Drive, Raleigh, North
Carolina. If you have any questions, please call meat 919-560-4386, ext.
35532
Sincerely,
LZ'z r/Z
Charles Cocker
Superintendent
Enclosures
Durham - Where Great Things Happen

,ar,
Toxicity Test Dates
South Durham Water Reclamation Facility
NCO047597
Sample Date Test Organism
12/14/2015
Fathead Minnow
pass
11/3/2015
Fathead Minnow
pass
10/6/2015
Ceriodaphnia dubia
pass
10/6/2015
Fathead Minnow
pass
7/7/2015
Ceriodaphnia dubia
pass
7/7/2015
Fathead Minnow
pass
4/7/2015
Ceriodaphnia dubia
pass
1/6/2015
Ceriodaphnia dubia
pass
10/7/2014
Ceriodaphnia dubia
pass
7/8/2014
Ceriodaphnia dubia
pass
4/22/2014
Ceriodaphnia dubia
pass
1/7/2014
Ceriodaphnia dubia
pass
10/8/2013
Ceriodaphnia dubia
pass
7/9/2013
Ceriodaphnia dubia
pass
4/2/2013
Ceriodaphnia dubia
pass
1/8/2013
Ceriodaphnia dubia
pass
10/9/2012
Ceriodaphnia dubia
pass
7/10/2012
Ceriodaphnia dubia
pass
4/16/2012
Ceriodaphnia dubia
pass
3/6/2012
Ceriodaphnia dubia
pass
2/28/2012
Ceriodaphnia dubia
pass
1/10/2012
Ceriodaphnia dubia
fail
10/11/2011
Ceriodaphnia dubia
pass
7/5/2011
Ceriodaphnia dubia
pass
4/19/2011
Ceriodaphnia dubia
pass
RECEivED/NCDEQ/DWR
FEB -1 2016
Water Quality
Permitting Section

DURHAM
In
1869
CITY OF MEDICINE
AM%
CITY OF DURHAM
DEPARTMENT OF WATER MANAGEMENT
101 CITY HALL PLAZA - DURHAM, NC 27701
919-560-4381 - FAX [phone]
www.durhamnc.gov
January 15, 2016
Administration
919-560-4381
Customer Billing Services
919-560-4412
RECEIVED/NCDEQ/DWR Laloratory Services 60 4386
FEB -1 Plant Maintenance
2016 919-560-4388
Water Quality
Permitting Section.
Environmental Sciences Branch
Division of Environmental Management
North Carolina Department of Environment and Natural Resources
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
SUBJECT: December 2015 Toxicity Testing
South Durham Water Reclamation Facility, NCO047597
Regulatory Compliance
919-560-4381
Utility Engineering
919-560-4381
Wastewater Treatment
North - 919-560-4384
South - 919-560-4386
Water Supply & Treatment
Williams - 919-560-4348
Brown - 919-560-4362
Water & Sewer Maintenance
919-560-4344
Please find attached the December toxicity results for the South Durham
Water Reclamation Facility, NC0047597. Please note that this test was the
Fat -head Minnow species only and it was required as part of our Permit
Renewal Application. The analyses were completed by ETT Environmental
Inc., 4 Craftsman Court, Greer, SC. through Pace Analytical Services Inc.,
6701 Conference Drive, Raleigh, North Carolina. If you have any questions,
please call me at 919-560-4386, ext. 35532
Sincerely,
Charles Cocker
Superintendent
Enclosures
Durham — Where Great Things Happen

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test '
I00b�
60
Facility: South Durham WRF NPDES # NC0047597
Laboratory: ETT Environmental, Inc.
x
Signature of Operator in Responsible Charge
x
Signature of Laboratory Su
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Pipe #: 001 County: Durham
90
pH (SU) IniUFin
DO (mg/L) IniUFin
Temp (C) IniUFin
10
9
10
10
10
10
10
10
0.7970
0.6660
0.6950
0.5450
10
10
10
10
10
10
10
10
0.6960
0.8730
0.9130
0.8710
10
10
10
10
10
10
10
10
0.8200
0.7890
0.9380
0.6500
9
10
10
10
10
10
10
10
0.7320
0.8530
0.9530
0.7780
10 10 10 10
10 10 10 10
10 10 10 10
10 10 10 10
8.2 / 7.4 7.9 / 7.3 7.8 / 7.4 7.9 1 7.5 8.0 / 7.5 7.9 / 7.5 8.0 ! 7.5
7.9 / 6.3 7.8 / 5.2 7.5 / 6.0 8.2 / 5.8 8.1 / 5.6 7.9 ! 6.1 7.2 / 6.8
24.8 / 24.9 24.9 / 24.8 24.4 1 24.9 24.9 1 24.7 25.11 25.2 24.5 ! 25.1 24.5 1 25.1
High Concentration 0 1 r 2 3 4 5 6
pH (SU) IniUFin
DO (mg/L) IniUFin
Temp (C) lniUFin
7.6 1 7.6 7.6 1 7.5 7.6 / 7.7 7.9 / 7.7 7.7 / 7.7 7.8 / 7.7 7.8 1 7.7
8.7 / 6.1 8.0 / 5.5 8.3 / 6.1 .3 I 6.3 8.5 / 5.9 8.0 I 5.7 7.5 I 6.3
25.0 / 24.9 25.0 / 24.8 24.3 / 24.9 ]2]4.7 ! 24.7 24.8 1 25.2 24.8 / 25.1 24.5 / 25.1
Sample 1 2 3 Survival Growth
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt ( C)
12-14-15
10 10 10 10
10 10 10 10
8.2 / 7.4 7.9 / 7.3 7.8 / 7.4 7.9 1 7.5 8.0 / 7.5 7.9 / 7.5 8.0 ! 7.5
7.9 / 6.3 7.8 / 5.2 7.5 / 6.0 8.2 / 5.8 8.1 / 5.6 7.9 ! 6.1 7.2 / 6.8
24.8 / 24.9 24.9 / 24.8 24.4 1 24.9 24.9 1 24.7 25.11 25.2 24.5 ! 25.1 24.5 1 25.1
High Concentration 0 1 r 2 3 4 5 6
pH (SU) IniUFin
DO (mg/L) IniUFin
Temp (C) lniUFin
7.6 1 7.6 7.6 1 7.5 7.6 / 7.7 7.9 / 7.7 7.7 / 7.7 7.8 / 7.7 7.8 1 7.7
8.7 / 6.1 8.0 / 5.5 8.3 / 6.1 .3 I 6.3 8.5 / 5.9 8.0 I 5.7 7.5 I 6.3
25.0 / 24.9 25.0 / 24.8 24.3 / 24.9 ]2]4.7 ! 24.7 24.8 1 25.2 24.8 / 25.1 24.5 / 25.1
Sample 1 2 3 Survival Growth
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt ( C)
12-14-15
8.2 / 7.4 7.9 / 7.3 7.8 / 7.4 7.9 1 7.5 8.0 / 7.5 7.9 / 7.5 8.0 ! 7.5
7.9 / 6.3 7.8 / 5.2 7.5 / 6.0 8.2 / 5.8 8.1 / 5.6 7.9 ! 6.1 7.2 / 6.8
24.8 / 24.9 24.9 / 24.8 24.4 1 24.9 24.9 1 24.7 25.11 25.2 24.5 ! 25.1 24.5 1 25.1
High Concentration 0 1 r 2 3 4 5 6
pH (SU) IniUFin
DO (mg/L) IniUFin
Temp (C) lniUFin
7.6 1 7.6 7.6 1 7.5 7.6 / 7.7 7.9 / 7.7 7.7 / 7.7 7.8 / 7.7 7.8 1 7.7
8.7 / 6.1 8.0 / 5.5 8.3 / 6.1 .3 I 6.3 8.5 / 5.9 8.0 I 5.7 7.5 I 6.3
25.0 / 24.9 25.0 / 24.8 24.3 / 24.9 ]2]4.7 ! 24.7 24.8 1 25.2 24.8 / 25.1 24.5 / 25.1
Sample 1 2 3 Survival Growth
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt ( C)
12-14-15
High Concentration 0 1 r 2 3 4 5 6
pH (SU) IniUFin
DO (mg/L) IniUFin
Temp (C) lniUFin
7.6 1 7.6 7.6 1 7.5 7.6 / 7.7 7.9 / 7.7 7.7 / 7.7 7.8 / 7.7 7.8 1 7.7
8.7 / 6.1 8.0 / 5.5 8.3 / 6.1 .3 I 6.3 8.5 / 5.9 8.0 I 5.7 7.5 I 6.3
25.0 / 24.9 25.0 / 24.8 24.3 / 24.9 ]2]4.7 ! 24.7 24.8 1 25.2 24.8 / 25.1 24.5 / 25.1
Sample 1 2 3 Survival Growth
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt ( C)
12-14-15
Sample 1 2 3 Survival Growth
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt ( C)
12-14-15
12-16-15
12-18-15
24 hr
24 hr
24 hr
64.0
48.0
48
69.0
70.7
80.4
528
536
500
<O.OS
0.07
<0.05
0.7
0.6
0.7
Normal
E-] no
yes
Hom. Var.
� yes
T_]
� yes
NOEC
100.0%
100.0%
LOEC
>100.0%
>100.0%
ChV
>100.0%
>100.0%
Method
Steels
Dunnetts
Test Organisms
Cultured In -House
X Outside Supplier
Hatch Date: 12/14
Hatch Time: 1600-1000
Overall Result
ChV
Stat Survival Growth
Conc.
Critical
Calculated
Critical
Calculated
Dilution H2O
45
10
20
2.41
-2.38
Hardness (mg/L)
50.0
67.5
10
20
2.41
-1.81
Alkalinity (mg/L)
40.4
90
10
20
2.41
-2.24
Conductivity (umhos/cm)
212
95
inn
10
in
20
In
2.41
-1.63
o A
_o Doi
DWQ Form A T-5 (1/04)
Page 2 of 9

Lab#
T46242
Client
South Durham WRF
Sample ID
PIPE 001
NPDES#
0
County
Durham
Month
12
Start & fad Date
16-Dec-15
Start & fad Time
1350
Started 3 fed By
AM
Test Organism
Pimephales romela
Noe. born data
<48 HRS
Nee. born time
ABS HATCH 12-14-1
TostTypo
NCCD
Dilution water
NC H2O 12-9-15
Units for Cone.
°%
%3rd BROOD
Test vassals
400 ml
Tostvolurne
150ml
Incubator#
1
Light
161V8dk
Initial Tomp'C
25
Artemis
750
Test method
EPA.21-R-02.019:1000
All reps have 10
!anisms unless noted otherwise.
)N TEMP
ITIAL-
24.6
kY 1-
24.9
kY 2 -
24.4
1Y 3-
24.9
1Y 4-
25.1
1Y 5-
24.5
N 6 -
24.5
Page 3 of 9

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Chain of Custody
E
;ace Analytical
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www.pacelabs.com
'Workorder: 92279894 Workorder Name: BIOASSAY 12/15/15 Results Requested 1/7/2016
:Report7Invoice To . _- = :: -
act
:SubcontrTo �= :.:.. '
Requested Analysis
Matthew Brainard
Pace Analytical Charlotte P.O.L) L01 t
9800 Kincey Ave. Suite 100
Huntersville, NC 28078
Phone [phone]
Email: [e-mail] `
' J
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Preseriied_Containers
Collect
9
Item Sample 1D` Date/Time _ Lab !D Matrlx . LAB USE ONLY
1 PIPE 001 12/15/2015 09:00 92279894001 Water X CPO
2
3
4
5
Comments
Transfers ReleasqdB. 1 D terrme Received By Date/Time
dhb 5 AZTg, 611 0
3 � D a-SSG��
Cooler Temperature on Receipt °C '-F-custody Seal Y or N I Received on Ice Y or N Samples Intact Y or N
in oroer to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
Tuesday, December 15, 2015 4:11:42 PM
FMT ALL-G002rev.00 2410arch2009 Pace 1 c.= ;

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Bioassay Chain of Custody Form
Y
Facility Name: ,� zw;2 jj
Address: ' e; 0. Box:
r �`t O tL?
ePhone # : B3 P (�� °'�
i d! C_ Contact: C, axe 5 0 P C% 'ti �A
CountY r✓. NPDES permit #: L�jj q 7 ZII�a-7pipe:
% Effluent Dilution (IWC): Plant Flow:
Select Test Method:
❑ C, dubia Pass/Fall Chronic 600CPFCNC El P,prometas Pass/Fail Acute 600A PFPpr
❑ C. dubia Full Range Chronic 600C FRCNC ❑ P ;promelas Full Range Acute 600A FRPpr
❑ C. dubia Pass/Fail Acute 600A PFCdu ❑ P.promelas Full Range Chronic 821C FRPpr
❑ M. bahia Pass/Fall Acute 600A PFMba
Sample Collector: Print-''�"' d�/o-�._ �'�''�A7
Signature _,�,•G.� ��,�"-"7
Sample Type:
Composite
Date Started .I-NVY'�Tlme:
Date Ended +• Ir Time:
Samples per Hr: # Hrs:
Grab
A •rPM
A or PM
Date: Time: AM or PM
Sample Volume: Chilled during Collectiod? Ye or No
Method of Transportation to the Lab:
r.hain of r'llefnrry
Received By
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2 0 ,la m Analytical, Inc. Use Only -
Pace Work Order Number: 2
Receiving Temperature: 2.0 Received By:
Pace Analytical Services, Inc. Address: 6701 Conference Dr., Raleigh, NC 27607 Phone: [phone]
Page 5 of 9

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PO Box 16414 Greenville SC 29606]4t4
(804) M 6942 (600) 8912325 Fax[phone]
Shipping Address: 4 Craftsman Cf. Greer, SC 29650
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COMPOSITE SAA/PLING PROCEDURES
TEhIPERATURE MONITORING PROCEDURES
HOLD VIE PROCEDURES
Composite samples must be collected over a 24 hour period.
Sample mmpemwm during collection and transport must be between
For toxicity testing the sample must first be toed within 36 hours
Time Proportional: I sample each (lour for 24 hours. Equal volw
0.0 and 6.0 °C. Samples must not be frozen. Use water ice in sealed baggs.
of sample collection (completion of composite sample).
or at minimum I sample every hours over 24 hows.
Sample may not be used after T3 hour, from sample collection.
Flow Pm ortional: As per instructions in NPDES permit.

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Workorder: 92279894
Workorder Name: RI(7AGSAV 17/1 rul r,
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mimpace/abs.c=
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Requestod'Analysis
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Matthew Brainard
Pace Analytical Charlotte P. p,
9800 Kincey Ave. Suite 100
Huntersville, NC 28078
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Phone [phone]
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LAB USE ONLY
1
PIPE 001
12/15/2015 09:00
92279894001
Water
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2
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Comments
Transfers
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Date ime
Received By
Date me
3
Cooler Temperature on Receipt *C Custody Seal Y or N Received on Ice Y or N Samples Intact Y or N
***In order to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
,5av,�.e Co l i e chi on waz (2A 1-7J 15 qA 0g oo (0LccorvC't'Lj To ajfw CQ C(-
Thursday, December 17, 2015 4:29:30 PM
FMT ALL-C-002rev.00 24March2009 Pace 1 01 1

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PO Box 16414, Greenville, SC 29606-7414
(884) VM942, [phone] Fax:[phone]
SoryPin9 Address: 4 Craftsman Ct, Greer, SC 29650
www.srrv+vwovMc�.�c.co.,
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CHAIN OFCUSTODY RECORD
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Client:
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COd9POSITESAMPLING PROCEDURES
TEAIFE'RATURE MONITORING PROCEDURES
HOLD TIME PROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must he hehveen
(For tosioity resting the sample uetst fir t b^ teed evithin 96 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volm
0.0 and 6.0 •C. Samples must not be frozen. Use water ice
in sealed bags.
of sampic collection (cump:c men of x.-. trusna sarnple,;.
or at minimum l sample every4 hours over 24 hours.
Sample may not be used after
72 hour. from sample collection.
Flow Proportional: A5 per instructions in NPDES permit
___

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Chain of Custody
Workorder: 92279894
Workorder Name: BIOASSAY 12/15/15
RPQ11I Q RPrnllocforl 117l'N11F
/5a'neAna1jdica1'9
m%v.pac&abscam
:Subcontract:: o=
RequestediArialyrsis
Matthew Brainard
Pace Analytical Charlotte P.O.
9800 Kincey Ave. Suite 100
Huntersville, NC 28078
Phone [phone]
Email: [e-mail]
;P,reserved`Containers
,
Item
Sample ID.
DateTme
Lab.ID "t
Matrix
z
LAB USE ONLY
i
PIPE 001
12/15/2015 09:00
92279894001
Water
x
2
3
4
5
1
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Re easeq�Bx
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Cooler Temperature on Receipt °C Custody Sea[ Y or N Received on Ice Y or N Samples Intact Y or N
***In order to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
Friday, December 18, 2015 3:38:57 PM
FMT ALL-C-002rev.00 24March2009 Poe i o�

DURHAM Administration
-
Customer Billing Services
CITY OF DURHAM 919-560-4412
DEPARTMENT OF WATER MANAGEMENT Laboratory Services
101 CITY HALL PLAZA . DURHAM, NC 27701 919-560-4386
919-560-4381 • FAX [phone] Plant Maintenance
www.durhamnc.gov 919-560-4388
1869 Regulat9ry Compliance
9-56014381
CrrY OF MEDICINE
- Utility Engineering
919-560-4381
Wastewater Treatment
North - 919-560-4384
South - 919-560-4386
Water Supply & Treatment
Williams - 919-560-4348
November 30, 2015 Brown - 919-560-4362
Water & Sewer Maintenance
919-560-4344
Environmental Sciences Branch
Division of Environmental Management
North Carolina Department of Environment and Natural Resources
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
SUBJECT: November 2015 Toxicity Testing
South Durham Water Reclamation Facility, NCO047597
Please find enclosed the November toxicity results for the South Durham
Water Reclamation Facility, NC0047597. Please note that this test was the
Fat -head Minnow species only and it was needed as part of our Permit
Renewal Application. The analyses were completed by ETT Environmental
Inc., 4 Craftsman Court, Greer, SC. through Pace Analytical Services Inc.,
6701 Conference Drive, Raleigh, North Carolina. If you have any questions;
please call me at 919-560-4386.
Sincerely,
Charles Cocker
Superintendent
Enclosures
Durham — Where Great Things Happen

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test
/",1
>�1
f�
Facility: South Durham WRF NPDES # NCO047597 Pipe #: 001 County: Durham
Laboratory: ETT Environmental, Inc. Comments
x
Signature 29=
Charge
x
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quulity
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Test Initiation Date/Time
04-Nov-15
! 1430
Avg WUSurv. Control 1.0513
% Eff. Repl.
1
2
3
4
Control Surviving #
9
9
10
9
% Survival 97.37%
Original #
45 Surviving #
Original #
W Uorlglnal (mg)
10
9
10
9
1.0640
1.D800
0.9660
1.0411
10
9
10
10
io
'10
io
10
1.1460
0.8880
1.2630
0.8050
Survival 97.5%
Avg Wt (mg) 1.0503
Wt/original
Wt/original
Wt/original
67.5 Surviving #
9
10
8
10
% Survival 100.0%
Original #
pH (SU) IniUFln
DO (mglL) lniUFln
Temp (C) (niUFin
10
10
10
10
10
9
10
10
10
9
'l0
10
10
i.0511
1.1950
1.0480
1.3410
8.2
! 7.3
7.9
I 7.4
8.1 1
7.9
7.8
I 7.4
7.7
1 7.4
7.9
! 7.5
7.9
I 7.6
7.9
/ 6.0
8.2
/ 6.6
8.0 /
7.3
7,9
1 5.6
7.5
/ 6.2
7.6
/ 6.2
7.5
I 5.1
24.6
! 25.0
25.0
! 26.1
24.5 1
24.8
24.0
1 24.7
24.7
1 25.1
24.e
1 25.1
24.7
1 24.5
High Concentration 0 1 2 3 4 5 B
ty
pH (SU) IniUFtn
DO (mg1L) lniUFin
e Temp (C) IntUFln
7.5
! 7.4
7.5
! 7.5
7.5
1 7.5
7.7
/ 7.3
7.3
1 7.4
7.6
I 7.4
7.8
1 7.6
8.4
! 6.8
8.1
1 6.5
8.0
/ 6.4
7.8
/ 5.3
8.3
I 5.3
7.7
1 4.8
7.5
1 6.2
25.0
1 25.0
24.7
1 25.1
24.4
1 24.8
26.0
1 24.7
24.8
1 26.1
24.7
! 25.1
24.5
/ 24,5
Test Organisms
OCultured in -House
0 X Outside Supplier
Hatch Date: <24 hrs
Hatch Time: 1600-1430
Sample 1 2 3 Survival Growth Overall Result
.40
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mglL)
Conductivi
(umhos/cm)
Chtorine(mg/L)
Temp at Receipt (C)
11-4-15
11-5-15
24 hr
24 hr
fi0.0
58.0
M
48.4
57.1
433
47?
0.05
0.16
0.0
0.7
Dilution H2O
Hardness (mglL) 50.0
Alkalinity (mglL) 46,80
Conductivity (umhos/cm) 211
Normal
® yes
XQ yes
ChV
Hom. Var.
Ej yes
X❑ yes
NOEC
100.0%
100.0%
LOEC
>100.0%
>100.0°k
ChV
>100.0%
>100.0%
Method
t Test
t Test
Stets
Survival
Growth
Cone.
Critical
Calcularod
Critical Calculated
45
2.41
-1.10
2.41
-0.11
67.5
2.41
-0.07
2.41
-1.08
90
2.41
-1.10
2.41
-0.98
95
2.41
0.48
2.41
0.08
100
2.41
-0.55
2.41
-1.09
9
10
8
10
DWQ Form AT-5 (1/04)
Page 2 of 9

Lab#
T46O30
Client
PACE ANALYTICAL
Sample t0
S. Durham WRF
NPOES#
NC
County
0
Month
11
start S fod Dato
04-Nov-15
Start d fod Time
1430
Startod & fod By
AM
Tost Organism
PEme hales pmmelai
Neo. bom data
<48 HRS
Noo. born time
ABS HATCH 11-2-1
Teat Typo
NCCD
Dilution Water
NC H2O 10-31-15
Untie for Cone.
%
%3rd BROOO
Tost vassals
400 ml
Tostvolume
150 ml
Incubator#
11
Light
161v8dk
InIUalTemp'C
25
Artemis
750
TO., method
EPA 921-R-02-013:1000
Page 3of9

Chain of Custody
;acieAnalyticalo
w►nvpacelabsccr.+
Workorder: 92274466 Workorder Name: BIOASSAY 11/03/2015
Report`/Invoice>Ta r , r _ Res
ults
equ 11 24/201Sbcontrac u
Taylor Ezell R6quested-Analysis" . „
Pace Analytical Charlotte P O.
9800 Kincey Ave. Suite 100 0
Huntersville, NC 28078
Phone [phone]
Email: [e-mail]
`Preserved Containers',. . --
F Collect 1
Item Sam le`lp. - z
Date/Time Lab iD
p
Matrix.. LAB USE ONLY
1 PIPE 001 11/3/2015 09:00 92274466001 Water X
° 2 (oo
3
°, 4
co
5
Transfers Reteas d By Comments
Date�me Received By Date/Ttme
2
3 1.1:::d L U�IS EZT r c 15, oq51
Cooler Temperature on Receipt °C Custody Seal Y or N Received on Ice Y or N Samples Intact Y or N
*"In order to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
Tuesday, November 03. 2015 3:47:15 PM FMT ALL-C-002rev.00 24March2009
Page i of 1

/I )
:'A
ce .
. ��7y�ic�71
Bioassay Chain Of Custody Form
Facility Name: i
Address:
P.O. Box:
Phone # : ��d�` ` 'e r q3 ,
Contact: A &-e j ker PX
County: �,� �NPDES permit #: ,%��, •� pipe: )�?
% Effluent Dilution (1WC):
Plant Flow:
Select Test Method:
❑ ;ddublaPasslFail Chronic 600CPFCNC❑ Full Range Chronic 600C FRCNC P'pr°melas Pass/Fail Acute600A PFPpr
❑ P.Pr°melas Full Range Acute 600A FRPpr
❑ Pass/Fail Acute 600A PFCduP.pramelas Full Range Chrortle 821C FRPpr
❑ Pass/Fail Acute 600A PFMba
Sample Collector: Print
Signa
Sam le T g
Composite
Date Started
Date Ended
Samples per Hr:
Grab
Time:
•• �"'' Time:
# Hrs:
l:l)A PM
i �M orr PM
Date: Time: ;Ii�'LAM or PM
Sample Volume:
Method of Transportation to the Lab:
Chilled during Collectiori?
&or No
EIT I D :' 4 6030 A
F r ac Analytical, Inc. Use Only
Pace Work Order Number:
Receiving Temperature:,
Received By: Lr�&
Pace Analyuca► Services, Inc. Address: 6701 Conference Ur., Raleigh, NC 27607 Phone: [phone]
I
Page 5 of 9

Chain Of Custody
Workorder: 92274466 Workorder Name: BIOASSAY 11/03/2015
Results RequestedRe ort/Invoice=To SubcontractToTaylor EzellReque
Pace Analytical Charlotte P O �� f a-of-'
�
9800 Kincey Ave. Suite 100 [
Huntersville, NC 28078
Phone [phone]
Email: [e-mail] : — I -
Preserved C
Collect a
Item Sample ID Dateme Labap fJtatrrX
1 PIPE 001 11/3/2015 09:00 92274466001 Water
� 2
os
c�
3
4
0
co 5
/,Aacie Analytical
i MVIV-Pacelabs.con
11 /24/2015
Analysis
Transfers RejeaseA D emme Comments
eceived By Comments
1)30
3 IL Tr
r03o
Cooler Temperature on Receipt 0.0 cc Custod Seal Y or N Received on Ice or N
Samples Intac -
fidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
***!n order to maintain client con
This chain of custody is considered complete as is since this information is available in the owner laboratory.
C-Alec-huy-1 -kryu was
9P
LAB USE ONLY
or N
Thursday, November 05, 2015 12:22:06 PM FMT ALL-G002rev.00 24March2009
Pace 1 of 1

�= acaAn�sytical
s
Bioassay Chain of Custody Form
Facility Name: ° --�,. ) r , D p�4go,_L
Address,���'y,s�l.�r.� P.O. Box:
Phone # : _...(
Contact: /101 x. 5` C 41r,
County: NPDES permit #: O `Y '75 9 7 pipe: c9 f
% Effluent Dilution (IWC):
Select Test Method:
❑
C. dubla Pass/Fail Chronic
600CPFCNC
❑
C. dubla Full Range Chronic
600C FRCNC
❑
C. dubla Pass/Fail Acute
600A PFCdu
❑
M. bahla Pass/Fail Acute
600A PFMba
Plant Flow:
t_I P.prornelas Pass/Fail Acute 600A PRPpr
❑ P.promelas Full Range Acute 600A FRPpr
❑ P.promelas Full Range Chronic 821C FRPpr
Sample Collector: Print >, e.Ma .
Signature X
Sample Type:
Composite
Date Started /-44Time: �j.�0� p or PM
Date Ended -�' j Time: Q Q) ;A%r PM
I Samples per Hr. # Hrs:�
Grab
Date: Time: AM or PM
Sample Volume: Chilled during Collection? Yes r No
Method of Transportation to the Lab:
• i`I•i�in of �`.ei•nrlu L�ole.�•:+e .
Relinquished y
Received By
r��WPloY
IM
M;
Comments:
f,V 19 It(Po 3D
Pe Analytical, Inc. Use Only -
Pace
Work Order Number: qladWRO
Receiving Temperature: Received By:
Pace Analytical Services, Inc. Address: 6701 Conference Dr., Raleigh, NC 27607 Phone: t919)834-4684
Va.
Page 7 of 9

-o
m
cc
m
co
0
CD
Chain Of Custody
Workorder: 92274466
Workorder Name;
BIOASSAY 11/03/2015
Results Requested
ace Analytical
►v►w.pacetabs.cam
11 /24/2015
r�GCC,vea on ice Y or N Samples Intact
"'In order to maintain cllent confidentiality, location/name of the sampling site, sampler's name and signature ma not be rov"
This chain of custody is considered complete as is since this information is available in the owner laboratory. y p ided on this COC document.
am pj e col l ec &i �� irne wti s a lro l l s a,f 9: oo 44-k, ,
SQz of4'w cac-
Friday, November 06, 2015 1:40:19 PM
FMT ALL-C-002rev 00 24March2009
Page 1 of

/006�
I'f1
Document Name: Date Revfsed: Juno 12. 2015
Bloassay Chaln of Custody Page 1 of 1
y Document Number
/_.>.•' ' Cei�llgPic l lssWngAulhodly:
FAAL-CS-007-rev.02 Paco Carolinas QuaHly Off co
I
Bioassay Chairs of Custody Form
Facility Name: •,buA JjeA1%
Address: i !- �%$��.g P.O. Box;
• d" Phone # i�A"— • �, �� ' 1 •3
Contact:
County:__ .���.�ied�'j NPDES permit #:� �! pipe: d:7
% Effluent Dilution (IWC): Plant Flow:
Select Test Method:
❑ C. dubia Pass/Fall Chronic 600CPFCNC P.promelas Pass/Fail Acute 600A PFPpr
❑ C. dubia Full Range Chronic 600C FRCNC ❑ P.Promelas Full Range Acute 600A FRPpr
❑ C. dubia Pass/Fail Acute 600A PFCdu ❑ P.promelas Full Range Chronic 821 C FRPpr
❑ M. bahla Pass/Fail Acute 600A PFMba `
Sample Collector. Print er W 4 L bof Akfiv
Signature
Sample Type:
Composite
Date Started -, .- j Time: � or PM
Date Ended f y �' Time; r PM
Samples per Hr: _ # Hrs:
Grab
Date: Time: AM or PM
Sample Volume: Chilled during Collection? Yes r No
Method of Transportation to the Lab:
r'k.3in of no 1M.—A., .
�-
Relinquished By
Roceived By
MWERWEA
VA
Coi.:merles:
EV i D -jwq(P00C'
• For Pace Analytical, Inc. Use only -
Pace Work Ord(ar Number:
"
Receiving Temperature: Received By:
Pace Analytical Services, Inc. Address: 6701 Conference Dr., Raleigh, NC 27607 Phone: [phone]
Page 9 of 9

Ava
Toxicity Test Dates
South Durham Water Reclamation Facility
NCOO47597
Sample Date Test Organism
12/14/2015
Fathead Minnow
pass
11/3/2015
Fathead Minnow
pass
10/6/2015
Ceriodaphnia dubia
pass
10/6/2015
Fathead Minnow
pass
7/7/2015
Ceriodaphnia dubia
pass
7/7/2015
Fathead Minnow
pass
4/7/2015
Ceriodaphnia dubia
pass
1/6/2015
Ceriodaphnia dubia
pass
10/7/2014
Ceriodaphnia dubia
pass
7/8/2014
Ceriodaphnia dubia
pass
4/22/2014
Ceriodaphnia dubia
pass
1/7/2014
Ceriodaphnia dubia
pass
10/8/2013
Ceriodaphnia dubia
pass
7/9/2013
Ceriodaphnia dubia
pass
4/2/2013
Ceriodaphnia dubia
pass
1/8/2013
Ceriodaphnia dubia
pass
10/9/2012
Ceriodaphnia dubia
pass
7/10/2012
Ceriodaphnia dubia
pass
4/1612012
Ceriodaphnia dubia
pass
3/6/2012
Ceriodaphnia dubia
pass
2/28/2012
Ceriodaphnia dubia
pass
1/10/2012
Ceriodaphnia dubia
fail
10/11/2011
Ceriodaphnia dubia
pass
7/5/2011
Ceriodaphnia dubia
pass
4/19/2011
Ceriodaphnia dubia
pass
DECEIVED/NCDEUDWR
FEB - 12016
Water Quality
Permitting Section

Administration
DURHAM 919-560-4381
Customer Billing Services
CITY OF DURHAM
919-560-4412
DEPARTMENT OF WATER MANAGEMENT
Laboratory Services
101 CITY HALL PLAZA • DURHAM, NC 27701
919-560-4386
919-560-4381 . FAX [phone]
Plant Maintenance
www.durhamnc.gov
919-560-4388
Regulatory
L n
18
OMa71C77NE
9-560I4381
CITY OF
Utility Engineering
919-560-4381
Wastewater Treatment
North - 919-560-4384
South - 919-560-4386
Water Supply & Treatment
Williams - 919-560-4348
January 15, 2016
Brown - 919-560-4362
Water & Sewer Maintenance
919-560-4344
Environmental Sciences Branch
Division of Environmental Management
North Carolina Department of Environment and Natural Resources
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
SUBJECT: December 2015 Toxicity Testing
South Durham Water Reclamation Facility, NCO047597
Please find attached the December toxicity results for the South Durham
Water Reclamation Facility, NCO047597. Please note that this test was the
Fat -head Minnow species only and it was required as part of our Permit
Renewal Application. The analyses were completed by ETT Environmental
Inc., 4 Craftsman Court, Greer, SC. through Pace Analytical Services Inc.,
6701 Conference Drive, Raleigh, North Carolina. If you have any questions,
please call me at 919-560-4386, ext. 35532
Sincerely,
4z
Charles Cocker
Superintendent
Enclosures
ow%
Durham — Where Great Things Happen

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test
Facility: South Durham WRF NPDES # NCO047597 Pipe #: 001 County: Durham
Laboratory: ETT Environmental, Inc. Comments
x
Signature of Operator in Responsible Charge
x
nature of
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Test Initiation Date/Time
16-Dec-15
/ 1350
Avg WUSurv. Control 0.6943
Eff. Repl.
1
2
3
4
Control Surviving #
%Survival 97.5%
Original #
WUoriginal (mg)
pH (SU) !niUFin
DO (mg/L) !niUFin
Temp (C) !niUFin
10
9
10
10
10
10
10
10
10
10
10
10
10
10
10
10
0.6960
0.8730
0
10
10
10
10
10
10
10
10
0.8200
0.7890
0.9380
0.6500
9
10
10
10
10
10
10
10
0.7320
0.8530
0.9530
0.7780
10
10
10
10
10
10
10
10
10
10
10
10
0.7830
0.8280
0.8530
1.0120
8.2
/ 7.4
7.9 /
7.3
7.8
/ 7.4
7.9
/ 7.5
8.0 /
7.5
7.9
J 7.5
8.0
/ 7.5
7.9
/ 6.3
7.8 /
5.2
7.5
! 6.0
8.2
/ 5.8
8.1 /
5.6
7.9
/ 6.1
7.2
/ 6.8
24.8
1 24.8
24.9 I
24.8
24.4
1 24.9
24.9
/ 24.7
25.1 1
25.2
24.5
1 25.1
24.5
1 25.1
High Concentration 0 1 2 3 4 5 6
pH (SU) IniUFin
DO (mg/L) Init/Fin
Temp (C) !niUFin
7.6
/ 7.6
7.6 /
7.5
7.6 /
7.7
7.9
/ 7.7
7.7 I
7.7
7.8
/ 7.7
7.8
I 7.7
8.7
/ 6.1
8.0 /
5.5
8.3 /
6.1
8.3
/ 6.3
8.5 /
5.9
8.0
/ 5.7
7.5
/ 6.3
25.0
1 24.9
25.0 /
24.8
24.3 /
24.9
24.7
1 24.7
24.8 1
25.2
24.8
/ 25.1
24.5
/ 25.1
Test Organisms
Cultured in -House
X Outside Supplier
Hatch Date: 12/14
Hatch Time: 1600-1000
Sample
1
2
3
Survival
Growth
Overall Result
Collection Start Date
12-14-15
12-16-15
12-18-15
Normal
E-] no
17 yes
ChV >100%
Grab
Hom. Var.
F-1 yes
� yes
Composite (Duration)
24 hr
24 hr
24 hr
NOEC
100.0%
100.0%
Hardness (mg/L)
64.0
48.0
48
LOEC
>100.0%
>100.0°k
Alkalinity (mg/L)
69.0
70.7
80.4
ChV
>100.0%
>100.0%
Conductivity (umhoslcm)
528
536
500
Method
Steels
Dunnetts
Chlorine(mg/L)
<0.05
0.07
<0.05
Temp at Receipt (C)
0.7
0.6
0.7
Slats
Survival
Growth
Conc.
Critical
Calculated
Critical
Calculatod
Dilution H2O
45
10
20
2.41
-2.38
Hardness (mg/L)
50.0
67.5
10
20
2.41
-1.81
Alkalinity (mg/L)
40.4
90
10
20
2.41
-2.24
Conductivity (umhoslcm)
212
95
10
20
2.41
-1.63
100
10
20
2.41
-2.83
DWQ Form AT-5 (1/04)
Page 2 of 9

Fecundity
N-Grav Gravid!
100
Id
NIMENMEIM
®®®
Lab#
T46242
Client
South Durham WRF
Sample ID
PIPE 001
NPOES#
0
County
Durham
Month
12
Start 6 fed Data
16-Dec-15
Start & fed Time
1350
Started & fad By
AM
Test Organism
Pimephales promela
No*. bom date
<48 HRS
No*. born time
ABS HATCH 12-14-1
Tost Typo
NCCD
Dilution water
NC H2O 12-9-15
Units for Conc.
%
%3rd BROOD
Test vassals
400 ml
Test volume
150 ml
Incubator#
1
Light
161U8dk
InitlaI Tom •C
25
Artemis
750
Test method
EPA 821•R-02-013:1000
All reps have 10
ganisms unless noted otherwise.
:ON TEMP
NITIAL-
24.6
MY 1-
24.9
MY 2 -
24.4
MY 3-
24.9
MY 4-
25.1
MY 5-
24.5
MY 6 -
24.5
Page 3 of 9

v
co
M
W,
CD
Chain of Custody
aceAnalX cal
tw pacelabs.corn
Workorder: 92279894 Workorder Name: BIOASSAY 12/15/15 Results Requested 1/7/2016
.once To --• �..,: .,�..:-
P. - _. .. • -; � .;r•::c tSubcorl#ract To � a _ =: �•: �; :, a = - - ..
Matthew Brainard Requested --*Analysis
Pace Analytical Charlotte P.O. WP-4 L) ' l
9800 Kincey Ave. Suite 100
Huntersviile, NC 28078
Phone [phone]
Email: [e-mail]
NJ
Rreserved `Contiiners"
- -•Collect
]tem: Sample 1D: Dateli'imeLab-
---1D Matrix LAB USE ONLY
1 PIPE 001 12/15/2015 09:00 92279894001 Water X
2
3
4
5
Comments .- . - ...
Transfers Releas d By D te/Time Received By Da eme
1 1 IS W b s15 16;
2
3 09 C D o.sSC-�-\
[Cooler Temperature on Receipt °C Custodv Seal Y or N Received on Ice Y or N Sam les Intact Y or N
in oraer to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
Tuesday, December 15, 2015 4:11:42 PM
FMT ALL-C-002rev.00 24March2009 Page : c` ,

ram\
i
Bioassay Chain of Custody Form
Facility Name: j4 ®� ���;e d lR� ,
Address: 1 ° e ; ��&R_O, Box:
AdAi*-. �14 i9 A � Phone # "{ 0 L13 ,P
✓o�� dli�:- Contact: --C1,Y1jriLe5 Cy C%'� &;
�� W
County :�xn �_ NPDES permit #: ed d/ 9 7plpe:,__.e)
% Effluent Dilution (lWC): Plant Flow:
Select Test Method: `
❑ C. dubla Pass/Fall Chronic 600CPFCNC P.promelas Pass/Fail Acute 600A PFPpr
❑ C. dubia Full Range Chronic 600C FRCNC ❑ P ;promelas Full Range Acute 600A FRPpi
❑ C. dubia Pass/Fail Acute 600A PFCdu ❑ P.promelas Full Range Chronic 821 C FRPpi
❑ M. bahia Pass/Fall Acute 600A PFMba
Sample Collector: Print
Signature
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Sample Type:
Composite
Date Started PAK
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Date Ended Time: •�� &? or PM
Samples per Hr: # Hrs: �U :W?
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Date: Time: AM or PM
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(•`h�ii, of l�...e.i.,.J., �..�..,.:...
Received By
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• Comments:
�22�0`��`e,Analytical, Inc. Use Only -
Pace
Work Order Number: 1 Ll
Receiving Temperature:r 2.0 Received By:
Pace Analytical Services, Inc. A9dress: 6701 Conference Dr., Raleigh, NC 27607 Phone: [phone]
Page 5 of 9

E,diTT
PO Box 16414 Greenville SC 2960GT414
(854) 8T7 6942 (80G) B91 2325 Far(864) 877 6935
ShippinmeAddress:
nn„ Craftsman Ct Greer, SC 29650
CUUODY RECORD
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COMPOSITE SAa/PLO.rO PROCEDURES
TEMPERATURE MONITORING PROCEDURES
HOLD TIME PROCEDURES
Composite samples a = be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must first be used vithin 36 hours
Time Proportional: I sample each hourfor 24 hours. Equal volm
0.0 and 6.0 °C. Samples must not be frozen. Use water ice in scaled bags.
of sample collection (completion of composite sample).
or at minimum I sample every 4 hours over 24 hours.
Sample may not be used after Tl )lour from sample collection.
Flow proportional: As per instructions in NPDES permit

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Workorder: 92279894
Workorder Name' RI(iAq.cZAV 1911 ti11 r,
laceAnalXicals
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'.Report/,InvoiceTo , : ".�` - Subcontract:To` `-
Matthew Brainard
Pace Analytical Charlotte p,p �,� �j�
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Huntersville, NC 28078
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1
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Date/Ttme
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Cooler Temperature on Receipt QJD *C Custody Seal Y or N Received on Ice Y or N Samples Intact Y or N
***In order to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
'5afnfU coll e cti O-Y�, wCO (A l-7115 wA 0900 (oLccorv('txj
Thursday, December 17, 2015 4:29:30 PM
FMT-ALL-C-002rev 00 24March2009 Papa 1 Q; 1

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I Box 10414, Greenville, SC 29805-7414
(8641877-6942• [phone] Fax[phone]
Shipping Address: 4 Cmftsman Ct• Greer. SC 29650
www_errcrvvirsvw.,e.mu.cvM
CHAIN OF CUSTODY RECORD
Page 0-
Client: � / ,r
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COMPOSIT£SAMPLINGPROCEDURES
TEM-ERATURE MOVIMRING PROCEDURES HOLD TIA9E PROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be between iFer toxicity tezEn2 the sa-mlemust firms be. usedndthin?(l hour
Time Proportional: I sample each hour for 24 hours. Equal volul
0.0 and 6.0 °C. Samples must not be frozen. Use haler ice in scaled bags. of sampto rat!ecden (co: nuic5en of cc .:poslie sample;:
or at minimum I sample every4 hours over 24 hours.
Sample may not be used after P_ hours from sample collection.
Flow Pro artional: As per instructions in NPDES permit.
,___

(n
Chain of Custody
Workorder: 92279894
Workorder Name: BIOASSAY 12/15/15
Rceeelfe Dtitle eric-+^,4 1r7P)A1r-
aceAnalytical
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Date/Time
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Cooler Temperature on Receipt , °C Custody Seal Y or N
Received on Ice Y or N Samples Intact Y or N
***In order to maintain client confidentiality, location/name of the sampling site, samplers name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
Friday, December 18. 2015 3:38:57 PM
FMT ALL-C-002rev.00 24March2009 page ; of

Administration
DURHAM 919-560-4381
Customer Billing Services
CITY OF DURHAM 919-560-4412
DEPARTMENT OF WATER MANAGEMENT Laboratory Services
101 CITY HALL PLAZA • DURHAM, NC 27701 919-560-4386
919-560-4381 . FAX [phone] Plant Maintenance
www.durhamnc.gov 919-560-4388
il Regulatory Compliance
1 8 6 9 919-560-4381
OIY OF MEDICINE
Utility Engineering
919-560-4381
Wastewater Treatment
North - 919-560-4384
South - 919-560-4386
Water Supply & Treatment
Williams - 919-560-4348
November 30, 2015 Brown - 919-560-4362
Water & Sewer Maintenance
919-560-4344
Environmental Sciences Branch
Division of Environmental Management
North Carolina Department of Environment and Natural Resources
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
SUBJECT: November 2015 Toxicity Testing
Aawk South Durham Water Reclamation Facility, NCO047597
Please find enclosed the November toxicity results for the South Durham
Water Reclamation Facility, NC0047597. Please note that this test was the
Fat -head Minnow species only and it was needed as part of our Permit
Renewal Application. The analyses were completed by ETT Environmental
Inc., 4.Craftsman COUrt, Greer, SC. through Pace Analytical Services Inc.,
6701 Conference Drive, Raleigh, North Carolina. If you have any questions,
please call me at 919-560-4386.
Sincerely,
Charles Cocker
Superintendent
Enclosures
Durham — Where Great Things Happen

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test
All
Facility: South Durham W RF NPDES If NC0047597 Pipe #;
Laboratory; ETT Environmental, Inc. Com
Signature of
Charge
County: Durham
MAILORICINALTO: Enviromecnlu1ScloncesOrunch
q
FEB -12016
Division or W ater Quality
NC DF.NR
1621 Mull Service Center
Water Quality
Ralelgh,NC 27699-1621
t3rmitSrg Becton
Test Initiation Date/Time 04-Nov-15
% Eff. Rapt. 1
Control Surviving #
Original #
W Ur[ginal (mg)
45 Surviving #
Original #
WUoriglnal(mg)
67.5 Surviving #
Original #
WUoriginal(mg)
90 Surviving #
Original #
W t/orl9lnal (mg)
95 Surviving #
Original #
WUodginal (mg)
100 Surviving #
Original #
WUoriglnal(mg)
Water Quality Data
Control 0 0
pH (SU) IniUFln
DO (mg/L) IniUFin
Temp (C) InIUFln
/ 1430 Avg WUSurv. Control i.o513
2 3 4
9
9
10
B
10
9
10
9
1.0640
1 1.0800
1 0.9660
1 1.0411
10
1 B
1 10
I 10
10
10
10
10
1.1460
1 0.9880
1 1.2630
1 0.8050
9
10
8
10
9
10
B
10
1.0711
1 1.1940
1 1.3889 1
0.9770
9
10
10
10
10
10
10
10
1.0880
1 1.1090
1 1.3420
1 1.0410
e
8
10
9
10
9
10
10
1.0910
1 1.0400
1 1.2230
0.7620
e
10
9
10
9
10
10
10
1.0511
1.1950
1 1.D480
1.3410
1 1 2 3
%Survives 97.37%
Avg W t (m9) 1.0378
% Survival
Avg WI (mg) 1.0503
%Survival 100.0%
Avg W t (mg) 1.1577
% Survival 97.5°/B
Avg Wt(mg) 1.1450
% Survival 92.31
Avg W t (mg) 1.0265
%Survival 97.44°/4
Avg W t (mg) 1.1588
4 5 6
8.2
/ 7.3
7.9
/ 7.4
8.1 I
7.9
17,8 f
7.4
17.7
/ 7.4
17.9 I
7.5
1 7.9 1
7.6
7.9
/ 6.0
8.2
/ 6.6
8.0 /
7.3
1 7.9 /
5.6
17.5
I 6.2
1 7.6 1
6.2
1 7.6 /
5.1
24A
1 26.0
25.0
/ 25.1
24.5 1
24.6
1 24.0 1
24.7
24.7
/ 25.1
1 24.5 1
25.1
24.7 1
24.5
High Concentration
0
1
2
3
4
5
6
pH (SU) Init/Fln
DO (mglL) IniUFin
Temp (C) InIUFin
Sample
Collection Start Dale
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (milli.)
Conductivity (umhos/cm)
Chlodne(mg/L)
Temp at Receipt ( C)
7.5 /
7.4
7.5
I 7.5
7.5
f 7.5
7.7
I 7.3
7.3
/ 7.4
7.6
/ 7.9
7.8
I 7.6
8.4 /
6.6
8.1
/ 6.5
8.0
/ 6.4
7.8
I 5.3
8.3
1 5.3
7.7
/ 4.8
7.5
/ 8.2
2S0 I
R6.0
24.7
I 26.1
24.0
I R4.B
26.0
/ 24.7R4.9
I 25.1
24.1
I 26.1
21.6
1 29.6
1 2 3
11.2-15
114-15
11-5-15
24 hr
24 hr
24 hr
52.0
60.0
58.0
57.0
48.4
57.1
454
433
477
<0.05
0.05
0.16
0.0
0.0
0.7
Dilution H2O
Hardness (mg/L)
50.0
Alkalinity (mg/L)
46.80
I
Conductivity (umhos/cm)
211
Survival
Growth
Normal
®yes
XQ yes
Hem. Var.
XQ yes
X❑yes
NOEC
100.0%
100.0%
LOEC
>100.0%
>100.0%
ChV
>100.0%
>100.0%
Method
tTest
West
Test Organisms
=Cultured In -House
QX Outside Supplier
Hatch Date: 424 hrs
Hatch Time: 1600-1430
Overall Result
ChV
State
Survival
Growth
Cone.
Critical
Calculated
Critical
Calculated
45
2.41
-1.10
2.41
-0.11
67.5
2.41
.0.07
2.41
-1.08
90
2.41
.1.10
2.41
-0.96
95
2.41
0.48
2.41
0.08
100
2.41
-0.55
2.41
-1.09
DWQ Form AT-5 (1104)
Page 2 of 9

N-Gray Gravid
�
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Lab#
T46030
Client
PACE ANALYTICAL
Sample iD
S. Durham WRF
NPDES#
NC
County
0
Month
11
Start & fed Data
04-Nov-15
Start 6 fed Time
1430
Started 3 fed By
AM
Test Organism
Plme hales promela.,
Nao. born date
a48 HRS
Nee. born time
ASS HATCH 11-2-1
Test Typo
NCCD
Dilution Water
NC H2O 10-31-15
Units for Cone.
%
%3rd BROOD
Test vessels
400 ml
Tostvolume
150mi
Incubator#
1
Light
16Itl8dk
Initial Tomp •C
25
Anemia
750
Test method
EPA 821.R-02-017:1000
Comments
All reps have 10
organisms unless noted otherwise.
CON TEMP
Initial-
24.6
DAY 1-
25
DAY 2 -
24.5
DAY 3-
24.8
DAY 4-
24.7
DAY 5 -
24.6
DAY 6 -
24.7
Page 3 of 9

v
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M
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0
to
Chain of Custody
;acieAnalytical's
m9w.pacelabs com
Workorder: 92274466 Workorder Name: 810ASSAY 11/03/2015
Report'! fnvoiceTo $ubcontractrTo
Results Requested 11124/2015
Taylor Ezell - - - Requested Analysis" --
Pace Analytical Charlotte P 0 � � • (, C2 j
9800 Kincey Ave. Suite 100 7 '7
Huntersville, NC 28078
Phone [phone] r'J -
Email: [e-mail]
Preserved Containers . 2=
Item Sample iD. DateMme :Lab ID: z
Matnx : `_
1 PIPE 001 LAB USE ONLY
11/3/2015 09:00 92274466001 Water X
2 I00
3
4
5
7T
. _ ,;• ;.:...-- ... -
Transfers Releas d By Comments
Date�me eceived By Date/ I ime
2
3 l 045' EiT tt f5IOq
Cooler Temperature on Receipt °C Custod Seal Y or N Received on Ice Y or N
Samples Intact Y or N
" *In order to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document
This chain of custody is considered complete as is since this information is available in the owner laboratory.
Tuesday, November 03, 2015 3:47:15 PM FMT ALL-C-002rev.00 24March2009
Page I of 1

401`1
/M)
Bioassay Chairs Of Custody Form
Facility Name:
Address: (�v'�I�f7r� P.O. Box;
Phone # 6
: �.or .
I�, ._ ev
�i . d Contact:
County: )e 4 NPDES pen -nit #; � � •%.,P�-��` •� i e: �
% Effluent Dilution (IWC): Plant Flow:
Select Test Method:
❑ C. dubia Pass/Fall Chronic 600CPFCNC
❑ C. dubia Full Range Chronic 640C FRCNC P.prornelas Pass/Fail Acute 600A PRPpr
❑ P.promelas Full Range Acute 600A FRPpr
❑ C. dubla Pass/Fall Acute 600A PFCdu ❑ P.promelas FuR Range Chronic 821C FRPpr
❑ M. bahla Pass/Falf Acute 600A PFMba
Sample Collector: Print
Signa
Sam e T e:
Composite
Date Started
Date Ended
Samples per Hr:
Grab
/'�•_ �'- Time: rI A r PM
• i Time: 3o 'M or PM
# Hrs: .
Date: Time: �AM or PM
Sample Volume:
Method of Transportation to the
Chilled during Collection?
&or No
ETC" ID -*46D3oA
Pace Work Order Number:F �f c Analytical, Inc. Use Only
Receiving Temperature: �, Received By:
Pace Analyltral Services, Inc. Address: 6701 Conference Dr., Raleigh, NC 27607 Phone: [phone]
Page 5 of 9

Chain Of Custody
Workorder: 92274466 Workorder Name: BIOASSAY 11/0312015 Results Requested
Report'/ lLvolce-;T-o'!:':--- ......
Taylor Ezell
Pace Analytical Charlotte
9800 Kincey Ave. Suite 100 P.ORM—fl ��10�
Huntersville, NC 28078
Phone [phone]
Email: [e-mail]
Preserved Containers
(D
0)
0
W
Item
r.
Sample*.ID
Collect
Date/Time -
:
LabID
0
M t Z a nx�
PIPE 001
1113/2015 09:00
92274466001
Water
ElEl
3
P4
5
5
ceAnalytical
mvivxacelabs.com
11/24/2015
Analysis -
Transfers �ejeasep)E�X DateMme ecdived By Comments
- x - k DateMme
1 f 14 T 1 11 IPA
41A YAIK nwkV
2
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11 IL93D
3 CAAbILA
Cooler Temperature on Receipt 0.0 cG-"-1—custody Seal Y or N —Received on Ice (X) or N Sam plesintaC&
***In order to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
C-ot (eCfi D-Y-1 -� (VU UdCtc.1 @
t 15 t S aDarn
op A
LAB USE ONLY
or N
Thursday, November 05, 2015 12:22:06 PM
FMT-ALL-C-002rev.00 24March2009 Page 1 of 1

^ 1
/001\t
Any Cal
0
Bioassay Chan of Custody Form
Facility Name: !-�-.•--
Address:,^ ��.�i e�� r3l' P.O. Box:
L� if_ AP1 he',L Al''ll Phone #: � � ��r� 0
C - d % Contact: Irk ", I e 5,` �'��C' re
County : •b d1 ,P_ /j ft•.;e-% NPDES permit #: 06. `Y "7.. I p�p e:
% Effluent Dilution (IWC):
Select Test Method:
❑ C. dubla Pass/Fall Chronic 600CPFCNC
❑ C. dubla Full Range Chronic 600C FRCNC
❑ C. dubla Pass/Fail Acute 600A PFCdu
❑ M. bahla Pass/Fail Acute 600A PFMba
Plant Flow:
U P.promelas Pass/Fail Acute 600A PFPpr
❑ P.Promelas Full Range Acute 600A FRPpr
❑ P.promelas Full Range Chronic 821C FRPpr
Sample Collector: Print,
Signature
Sample Type:
Composite
Date Started Time:
Date Ended - j Time:
Samples per Hr: # Hrs:
Grab
0 Agor PM
�r�•!1 A r PM
. L .
Date: Time: AM or PM
i
Sample Volume: Chilled during Collection?
Method of Transportation to the -Lab:
(YeOr No
MCA:
Film
Comments: '
6V tP '*O� )D Q
For Pace Analytical, Inc. Use Only -
Pace Work Order Number:'
Receiving Temperature: Received By:
Pace Analytical Services, Inc. Address: 6701 Conference Dr„ Rafeigh, NC 27607 Phone: [phone]
Va
Page 7 of 9

v
m
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Chain Of Custody
Workorder: 92274466 Workorder Name:
BIOASSAY 11/03/2015
Results Requested
ace Analytical
imvrr.PaCVab&c=
11 /24/2015
��ceiveo on ice Y ***!n order to maintain client confidentiality, location/name of the sampling site, sampler's or N Sam Ies Intact '
name and signature ma not be rov'
This chain of custody is considered complete as is since this information is available in the owner laboratory, y p Ided on this COC document.
ampke collec�ovi dAfel-fia wqs alfalls RA q:c)o 4*3,% .
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Friday. November 08, 2015 1:40:19 PM
FMT ALL-C-002rev.00 24March2009
Page 1 of 1

Dacu<nenl Name; Date Revised., June 12. 2015
Bloassa Chain of Custody Pao 1 of i
w Namnant NunZer.Issuing eAn. tlytic��J F-RAL-CS•007.rov o2 Paco Carolsnas Oua itx OfRco
Bioassay Chain of Custody Form
Facility Name: ' ,�% �t���e,-� �' , -
Address: A
_ ���iC?� ��'$� P.O. Box:
Phone # .—��d"-- •��� ' �J
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County : _D,46 A942 NPDES permit #: R'7 pipe: Od
% Effluent Dilution (1WC): Plant Flow:
Select Test Method: f
❑ C. dubia Pass/Fail Chronic 600CPFCNC P.prornelas Pass/Fail Acute 600A PFPpr
❑ C. dubia Full Range Chronic 600C FRCNC ❑ P.Promelas Full Range Acute 600A FRPpr
❑ C. dubia Pass/Fail Acute 600A PFCdu ❑ P.promelas Full Range Chronic_ 821 C FRPpr
❑ M. bahla Pass/Fail Acute 600A PFMba
Sample Collector. Print C' r � Ivef L 4ff - In'?
• Signature �v
Sample Tytse:
Composite
Date Started
Date Ended
Samples per Hr:
Grab
- 1 Time:
Time:
# Hrs:
/C0d2or PM
M rPM
Date: Time: AM or PM
Sample Volume: Chilled during Collection? Yes r No
Method of Transportation to the Lab:
Relinquished By
Date
Time
Received By'
Date
Time44
Coi.:mertts:
For Pace Analytical, Inc. Use Only -
Pace Work Order Number:
Receiving Temperature: Received By:
Pace Analytical Services, Inc. Address: 6701 Conference Dr., Raleigh, NC 27607 Phone: [phone]
Page 9 of 9

Toxicity Test Dates
� South Durham Water Reclamation Facility RECEIVED/NCDE4/DWR
NCO047597
Sample Date Test Organism
12/14/2015
Fathead Minnow
pass
11/3/2015
Fathead Minnow
pass
10/6/2015
Ceriodaphnia dubia
pass
10/6/2015
Fathead Minnow
pass
7/7/2015
Ceriodaphnia dubia
pass
7/7/2015
Fathead Minnow
pass
4/7/2015
Ceriodaphnia dubia
pass
1/6/2015
Ceriodaphnia dubia
pass
10/7/2014
1 Ceriodaphnia dubia
pass
7/8/2014
Ceriodaphnia dubia
pass
4/22/2014
Ceriodaphnia dubia
pass
1/7/2014
Ceriodaphnia dubia
pass
10/8/2013
Ceriodaphnia dubia
pass
7/9/2013
Ceriodaphnia dubia
pass
4/2/2013
Ceriodaphnia dubia
pass
1/8/2013
Ceriodaphnia dubia
pass
10/9/2012
Ceriodaphnia dubia
pass
7/10/2012
Ceriodaphnia dubia
pass
4/16/2012
Ceriodaphnia dubia
pass
3/6/2012
Ceriodaphnia dubia
pass
2/28/2012
Ceriodaphnia dubia
pass
1/10/2012
Ceriodaphnia dubia
fail
10/11/2011
Ceriodaphnia dubia
pass
7/5/2011
Ceriodaphnia dubia
pass
4/19/2011
Ceriodaphnia dubia
pass
FEB - 1 2016
Water Quality
Permitting Section

Administration
DURHAM 919-560-4381
Customer Billing Services
CITY OF DURHAM
919-560-4412
DEPARTMENT OF WATER MANAGEMENT
Laboratory Services
il
101 CITY HALL PLAZA • DURHAM, NC 27701
919-560-4386
919-560-4381 . FAX [phone]
Plant Maintenance
www.durhamnc.gov
919-560-4388
Regulatory Compliance
1869
919-560-4381
CITY OF MEDICINE
Utility Engineering
919-560-4381
Wastewater Treatment
North - 919-560-4384
South - 919-560-4386
Water Supply & Treatment
Williams - 919-560-4348
January 15, 2016
Brown - 919-560-4362
Water & Sewer Maintenance
919-560-4344
Environmental Sciences Branch
Division of Environmental Management
North Carolina Department of Environment and Natural Resources
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
SUBJECT: December 2015 Toxicity Testing
South Durham Water Reclamation Facility, NCO047597
Please find attached the December toxicity results for the South Durham
Water Reclamation Facility, NC0047597. Please note that this test was the
Fat -head Minnow species only and it was required as part of our Permit
Renewal Application. The analyses were completed by ETT Environmental
Inc., 4 Craftsman Court, Greer, SC. through Pace Analytical Services Inc.,
6701 Conference Drive, Raleigh, North Carolina. If you have any questions,
please call me at 919-560-4386, ext. 35532
Sincerely,
� '' 4zz
Charles Cocker
Superintendent
Enclosures
1
Durham — Where Great Things Happen

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test
f�
Facility: South Durham WRF NPDES # NCO047597
Laboratory: ETT Environmental, Inc.
Signature of Operator in Responsible Charge
x
of
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Pipe M 001 County: Durham
Test Initiation Date/Time
16-Dec-15
/ 13li0
Avg WUSurv. Control 0.6943
Eff. Repl.
1
2
3
4
Control Surviving #
%Survival 97.5%
Original #
WUoriginal (mg)
Avg Wt (mg) 0.6758
45 Surviving #
%Survival 100.0%
Original #
Wt/original (mg)
0.6960
0.8730
0.9130
Avg Wt (mg) 0.8383
67.5 Surviving #
%Survival 100.0%
Original #
Wt/original (mg)
Avg Wt (mg) 0.7993
90 Surviving #
%Survival 97.5%
Original #
WUoriginal (mg)
Avg Wt (mg) 0.8290
95 Surviving #
%Survival 100.0%
Original #
Wt/original (mg)
0.7530
0.8250
0.8380
0.7330
Avg Wt (mg) 0.7873
100 Surviving #
%Survival 100.0%
Original #
Wt/original (mg)
Avg Wt (mg) 0.8690
Water Quality Data
pay
Control
0
1
2
3
4 5 6
pH (SU) lniUFin
DO (mg/L) IniUFin
Temp (C) IniUFin
10
9
10
10
10
10
10
10
0.7970
0.6660
0.6950
0.5450
10
10
10
10
10
10
10
10
0.8710
10
10
10
10
10
10
10
10
0.8200
0.7890
0.9380
0.6500
9
10
10
10
10
10
10
10
0.7320
0.8530
0.9530
0.7780
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
0.7830
0.8280
0.8530
1.0120
8.2
1 7.4
7.9
/ 7.3
7.8 /
7.4
7.9
/ 7.5
8.0
/ 7.5
7.9
/ 7.5
8.0
1 7.5
7.9
/ 6.3
7.8
J 5.2
7.5 !
6.0
8.2
! 5.8
8.1
1 5.6
7.9
! 6.1
7.2
/ 6.8
24.6
/ 24.9
24.9
/ 24.8
24.4 !
24.9
24.9
/ 24.7
25.1
/ 25.2
24.5
1 25.1
24.5
J 25.1
High Concentration 0 1 2 3 4 5 6
pH (SU) IniUFin
DO (mgJL) IniUFin
Temp (C) IniUFin
7.6
/ 7.6
7.6
/ 7.5
7.6 /
7.7
7.9
/ 7.7
7.7
/ 7.7
7.8 /
7.7
7.8
/ 7.7
8.7
/ 6.1
8.0
1 5.5
8.3 1
6.1
8.3
/ 6.3
8.5
! 5.9
8.0 /
5.7
7.5
/ 6.3
25.0
/ 24.8
25.0
/ 24.8
24.3 J
24.9
24.7
J 24.7
24.8
J 25.2
24.8 f
25.1
24.5
1 25.1
Test Organisms
(_;Cultured In -House
X Outside Supplier
Hatch Date: 12/14
Hatch Time: 1600_1000
Sample
1
2
3
Survival
Growth
Overall Result
Collection Start Date
12-14-15
12-16-15
12-18-15
Normal
0 no
� yes
ChV
Grab
Hom. Var.
F71 yes
yes
Composite (Duration)
24 hr
24 hr
24 hr
NOEC
100.0%
100.0%
Hardness (mg/L)
64.0
48.0
48
LOEC
>100.0%
>100.0%
Alkalinity (mg/L)
69.0
70.7
80.4
ChV
>100.0%
>100.0%
Conductivity (umhos/cm)
528
536
500
Method
Steels
Dunnetts
Chlorine(mglL)
<0.05
0.07
<0.05
Temp at Receipt (C)
0.7
0.6
0.7
Stats
Survival
Growth
Conc.
Critical
Calculated
Critical
Calculated
Dilution H2O
45
10
20
2.41
-2.38
Hardness (mg/L)
50.0
67.5
10
20
2.41
-1.81
Alkalinity (mg/L)
40.4
90
10
20
2.41
-2.24
Conductivity (umhoslcm)
212
95
10
20
2.41
-1.63
100
10
20
2.41
-2.83
DWQ Form AT 5 (1/04)
Page 2 of 9

Fecundity
N-Grav Gravid
ra
t
Lab#
T46242
Client
South Durham WRF
Sample ID
PIPE 001
NPOES#
0
County
Durham
Month
12
Start & fad Data
16-Dec-15
Start 3 fad Time
1350
Started a fad By
AH
Test Organism
Pime hales promele
Noo. born data
<48 HRS
Noo. born time
ABS HATCH 12-14-1
Test Typo
NCCD
Dilution Water
NC H2O 12-9-15
units for Cone.
%
%3rd BROOD
Test vessels
400 ml
Test volume
150 ml
Incubators«
1
Light
161t/8dk
initial Tamp •C
25
Artamia
750
Tcst method
I EPA e21.R-02-013:1000
All reps have 10
organisms unless noted otherwise.
CON TEMP
INITIAL-
24.6
DAY 1-
24.9
DAY 2 -
24.4
DAY 3-
24.9
DAY 4-
25.1
DAY 5-
24.5
DAY 6 -
24.5
Page 3 of 9

W
Chain of Custody
Workorder: 92279894
Workorder Name: BIOASSAY 1911 r,19 r,
;acieAnaVical
wv pnelabsxam
-----in oraer to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is avallable in the owner laboratory.
ruesday, December 15, 2015 4:11:42 PM
FMT ALL-C-002rev.00 24March2009 Parle 1 of

l�
F
i
Bioassay Chain of Custody Form
Facility Name: i Irk a= ,�i' 02 �j d(.<%Z�l2f
Address: �'r�_ ,� Y40,0, •' ,;f��P,0, Box:
Phone # : __. _ � Y3 P L
Contact: _ L' �1JX L e `: h
Count �'
a
y = NPDES permit #: 0a' �46 pipe:
% Effluent Dilution (lWC): Plant Flow:
Select Test Method:
❑ C. dubla Pass/Fail Chronic 600CPFCNC P.Promelas Pass/Fall Acute 600A PRPpr
❑ C. dubia Full Range Chronic 600C FRCNC ❑ P.promelas Full Range Acute 600A FRPpr
❑ C. dubla Pass/Fail Acute 600A PFCdu ❑ P.promelas Full Range Chronic 821C FRPpr
❑ M. bahia Pass/Fail Acute 600A PFMba
Sample Collector: Print a,1/4-441S
Signature
Sample Type:
Composite
Date Started / ,//'Time: : t}i� A • r PM
Date Ended Time: jQ? A or PM 4
Samples per Hr: # Hrs:
Grab
Date: Time: AM or PM
Sample Volume: Chilled during Collectiod? CYjeor No
Chain of ri ictnrty Rataacc
Received By
now,AN
'Ail IMF
ANN
M
NE
0 �A66mlmff*
MOR a
Comments:
.� qi� 94RA
o , a L Analytical, Inc. Use Only -
Pace Work Order Number: Oil
2
Receiving Temperature: �, 0 Received By:
Pace Analytical Services, Inc. Address: 6701 Conference Dr., Raleigh, NC 27607 Phone: [phone]
Page 5 of 9

PO Box 16414 Greenville, SC 2980E-7414
[phone], [phone] Fmc(864) 877 6938
shipping Address: 4 Craftsman Ct, Greer, SC 29650
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CHAT OF C�JS' ODD CORD
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-------------
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Spacial instructions:
Sample Custody Transfer Record C
Secure
Receipt
Sample
Date Time Resin uahcdBy/ Orminization
Area
Tamp°C
Preserved?
i -18'• 6
GOO
2+e red
V
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I
COMPOS/TE SAMPLING PROCEDURES
TEA•1PERATUREA50N/TORING PPOCEDURES
HOLD TIME PP,OCEDUPES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be between
For toxicity testing the sample must firs. be used within 36 hours
Time Proportional: 1 sample each hour for 24 hours. Equal volm
0.0 and 6.0 °C. Samples must not be frozen. Use stater ice in sealed bags.
of sample collection (completion of composite sample).
or at minimum I sample every 4 boors over 24 hours.
Sample may not be used after 72 hours from sample collection.
Flow Proportional: As r instructions in NPDES ermit
_J

co
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Chain of Custody p
aloe Analytical
! ►v ovpacelabsxom
Workorder: 92279894 Workorder Name: BIOASSAY 12/15/15 Results Requested 1/7/2016
-.Re ort /InvoiceTo- . _
p .. _:- - ::.:. ;,. Requested'Analysis
Matthew Brainard
Pace Analytical Charlotte p,p.i� Lf/
9800 Kincey Ave. Suite 100
Huntersville, NC 28078 0
Phone [phone]
Email: [e-mail]
Preserved Cantaiiiers `� . c
Collect,a. , V,
Item: ' SampJe ID; DatelTtme ' Lab lD Matrix -
LAB USE ONLY
1 PIPE 001 12/15/2015 09:00 92279894001 Water X a
2
3
4
5
Comments
Transfers Released I y Daterime Received By DateMme
b stc�
2 p
3
Cooler Temperature on Recei t °C r."stody Seal Y or N Received on Ice Y or N Samples Intact Y or N
***In order to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
,5a0,pjt Co l i e w( 1-7 t s A4 pg oo (0LcC0r0(-,Ll' -im aj/ 4 4 CO C�. - r3D
Thursday, December 17, 2015 4:29:30 PM FMT ALL-C-002rev.00 24March2009 Pane 1 of 1

ETI
,t -MINIA
PO Box 16414 Greenville SC 29806.7414
(864) 87M942 [phone] 1`2c(864) 877 6938
Shipping Address: 4 Craftsman Ct, Greer, SC 29650
.waw.¢rrc�..nno..w,e�rwi.c=n
CHAIN OF CUSTODY RECORD
Page of
11,14
Client:
Cr'f` pi afaddl� $ /� CJ�Z h
Prngmm
Containers
presanative
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Facility: / 5 fb/i 9 �n
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Sample Custody Transfer Record
(Area Secure I
Receipt
I Sample
's ' , 1
Date me Relinquished By ! Or=anization %" ei ,tr n f,
Time
Temn eC
Pre:e- ed1
l/ V•
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COMPOSITES4MPLIRG PROCEDURES
TEAfPERATURE MORTPORAIGPROCE•DURES HOLD TIAIEPROCEDURES
Composite samples must be collected over a 24 hour period.
Sample temperature during collection and transport must be bemna-r.. I Fer nutici:,• -sting the sample :Hutt first bs used nrithi i 26 hour
Time Proportional: 1 sample each hour for 24 hours. Equal vohn
0.0 and 60 *C. Samples most not be frozen. Use water ice in sealed bags. of Sample collection (comulcnen of coc: vvWile sarlplc;.
or at minimum I sample every4 hours over 24 hours.
Sample may not be used afier? (tours from sample collection.
Flow Proorlienal: As per instructions in M'DES permit.
______

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to
m
cc
0
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Chain Of Custody
Workorder: 92279894
Workorder Name: BIOASSAY 12/15/15
Pncrrlfe L?nnrrft,-f- J -1 r7113n-ia
2aneAnalytical
i
mvw.pace/ab&cam
-- ;Subcontract -To'°
_ _ _._
_=RequestedArialysis
.
_
Matthew Brainard
Pace Analytical Charlotte
P O,
9800 Kincey Ave. Suite 100
Huntersville, NC 28078
Phone [phone]
Email: [e-mail]
'i'-'P.reserved=Containers;::`.
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12/15/2015 09:00
92279894001
Water
X
2
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4
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Transfers
Re ease %
Dale ime
Received By
D e/Time
�� Q,fJ
r, i
tz
2
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1
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'
Cooler Temperature on Recei t , °C Custody
Seal Y or N
Received on Ice Y or N Samples Intact Y or N
***In order to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
Friday, December 18, 2015 3:38:57 PM
FMT ALL-C-002rev.00 24March2009 Pace 1 O�F 1

DURHAM Administration
19-560 438
Customer Billing Services
CITY OF DURHAM 919-560-4412
DEPARTMENT OF WATER MANAGEMENT Laboratory Services
101 CITY HALL PLAZA • DURHAM, NC 27701 919-560-4386
919-560-4381 . FAX [phone] Plant Maintenance
www.durhamnc.gov 919-560-4388
1869 Regulatory
CFrY OF MEOIONE Utility Engineering
919-560-4381
Wastewater Treatment
North - 919-560-4384
South - 919-560-4386
Water Supply & Treatment
Williams - 919-560-4348
November 30, 2015 Brown - 919-560-4362
Water & Sewer Maintenance
919-560-4344
Environmental Sciences Branch
Division of Environmental Management
North Carolina Department of Environment and Natural Resources
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
SUBJECT: November 2015 Toxicity Testing
South Durham Water Reclamation Facility, NCO047597
Please find enclosed the November toxicity results for the South Durham
Water Reclamation Facility, NC0047597. Please note that this test was the
Fat -head Minnow species only and it was needed as part of our Permit
Renewal Application. The analyses were completed by ETT Environmental
Inc., 4 Craftsman Court, Greer, SC. through Pace Analytical Services Inc.,
6701 Conference Drive, Raleigh, North Carolina.. If you have any questions,
please call me at 919-560-4386.
Sincerely,
Charles Cocker
Superintendent
Enclosures
Durham — Where Great Things Happen

Effluent Toxicity Report Form -Chronic Fathead Minnow Multl-Concentration Test
Facility: South Durham WRF NPDES # NCO047597 Pipe M 001 County: Durham
Laboratory: ETT Environmental, Inc. Comments
x
Signature of
x
Signature of
Charge
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mall Service Center
Raleigh, NC 27699-1621
Test Initiation Date/Time
04-Nov-15
1 1430
Avg WtlSurv. Control 1.0613
% Eff. Repl.
1
2
3
4
Contro!j Surviving it
9
9
10
9
%Survival 97.37%
Original #
10
9
1
pH (SU) tniUFin
DO (mg/L) tnlUFtn
Temp (C) tniUFtn
0
9
10
9
10
10
9
10
8
10
9
10
10
10
10
10
10
10
1.0880
1.1090
1.3420
1.0410
9
8
10
9
10
9
10
10
1.0910
1.0400
1.2230
0.7620
9
10
9
10
9
10
10
10
1.0511
1.1950
1.0480
1.3410
8.2
! 7.3
7.9
! 7.4
8.1
1 7.9
7.8
! 7.4
7.7
I 7.4
7.9
! 7.5
7.8
1 7.6
7.9
! 6.0
8.2
/ 6.6
8.0
/ 7.3
7.9
! 5.6
7.5
/ 6.2
7.6
I 6.2
7.5
! 5.1
24.8
/ 25.0
25.0
1 25.1
24.6
I 24.8
24.8
I 24.7
24.7
1 25.1
24.8
J 25.1
24.7
/ 24.5
High Concentration 0 1 2 3 4 5 6
pH (SU) iniUFin
DO (mg1L) IniUFtn
Temp (C) lNUFtn
7.5
!
7.5
! 7.5
7.5
/ 7.5
7.7 /
7.3
7.3
! 7.4
7.6
1 7.4
7.8
! 7.6
8.4
J7.4
!
8.1
1 8.5
8.0
/ 6.4
7.8 /
5.3
8.3
! 5.3
7.7
1 4.8
7.5
I 6.2
25.8
I
24.7
J 25.1
24.4
I 24.8
25.0 I
24.7
24.8
I 26.1
24.7
I 25.1
24.6
I 24.5
Test Organisms
=Cuitured In -House
Q X Outside Supplier
Hatch Date: <24 hrs
Hatch Time: 1600-1430
Sample
1
2
3
Survival
Growth
Overall Result
Collection Start Date
11-2-15
11.4-15
11-5-15
Normal
®yes
XQ yes
ChV >100%
Grab
Hom. Var.
Ej yes
X❑ yes
Composite (Duration)
24 hr
24 hr
24 hr
NOEL
100.0%
100.0%
Hardness (mglL)
52.0
60.0
58.0
LOEC
>700.0°%
>100.0°%
Alkalinity (mglL)
57.0
48.4
57.1
ChV
>100.0°%
>100.0°%
Conductivity (umhoslcm)
454
433
477
Method
t Test
t Test
Chlorine(mg/L)
<0.05
0.05
0.16
Temp at Receipt (C)
0.0
0.0
0.7
Stats
Survival
Growth
Cone.
Critical
Calculated
Critical
Calculated
Dilution H2O
45
2.41
-1.10
2.41
-0.11
Hardness (mg1L)
50.0
67.5
2.41
-0.07
2.41
-1.08
Alkalinity (mglL)
46,80
90
2.41
-1.10
2.41
-0.98
Conductivity (umhoslcm)
211
95
2.41
0.48
2.41
0.08
100
2.41
-0.55
2.41
-1.09
DWQ Form AT-5 (1/04)
Page 2 of 9

Fecundity
e^
Lab#
T46030
Client
PACE ANALYTICAL
Sample ID
S. Durham WRF
NPDES#
NC
County
0
Month
11
Start d fed Date
04-Nov-15
Start E fed Time
1430
Started & fad By
AM
Test Organism
Pime hates promela.,
Neo. born data
<48 HRS
Neo. born time
ABS HATCH 11-2-1
Toat Typo
NCCD
Dilution Water
NC H2O 10-31-15
UnitsforConc.
%
%Jrd BROOD
Teat vessels
400 ml
Test volume
150 ml
Incubator#
1
Light
161t18dk
Initial Tamp `C
25
Artemis
750
Toot method
EPA 871-R-02-013:1000
Comments
All reps have 10
organisms unless noted otherwise.
CON TEMP
Initial-
24.6
DAY 1-
25
DAY 2 -
24.5
JDAY 3-
24.8
DAY 4-
24.7
DAY 5 -
24.6
DAY 6 -
24.7
Page 3of9

A
0
cc
Chain of Custody
0
_ aCEAnali/f7Cal
vAmpacelabsaor,i
Workorder: 92274466 Workorder Name: BIOASSAY 11/03/2015
Report! �:S,ubc:..... :..f.::
ResultsRequested11l24/2015e%ontract;To`
'
Requesti&Analysis:.; ..
Taylor Ezell _ -..
Pace Analytical Charlotte P.O.=j%?�—
9800 Kincey Ave. Suite 100
Q
Huntersville, NC 28078 4!
Phone [phone]
Email: [e-mail]
:Preserved Containers
Collect rd
a
Item Sample ID _ Date/ rime. Lab.,
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1 PIPE 001 LAB USE ONLY
11/3/2015 09:00 92274466001 Water X
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u INV,M faw Gr
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, � .
3 �.t ETT tc 1510y
Cooler Temperature on Receipt °C Custod Seal Y or N Received on Ice Y or N samples Intact Y or N
***In order to maintain client confidentiality, location/name of the sampling site,_ sampler's name and signature may not be provided on this COC document
This chain of custody is considered complete as is since this information is available in the owner laboratory.
Tuesday, November 03, 2015 3:47:15 PM FMT ALL-C-002rev.00 24March2009 Page 1 or 1

/011
6a
Bioassay Chair of Custody Form
Facility Name:
Address: (0�' 6 ���7t'�b P.O. Box.
Phone # : q
a
2"�� Contact:
County: i e �'��. NPDES permit #:
% Effluent Dilution (IWC): Plant Flow:
Select Test Method: =db
slFail Chronic 600CPFCNC P.promelas Pass/Fail Acute 600A PFP r
Range Chronic 600C FRCNC ❑ P.promelas Full Range Acute 600A FRPpr
s/Fall Acute 600A PFCdu ❑ P.promelas Full Range Chronic 821C FRPpr
❑ M. bahla PasslFail Acute 600A PFMba '
Sample Collector: Print
Signa
Sample Tvoe•
Composite
Date Started
Time: r SA r PM
Date Ended
Samples per Hr:
' •.,�•. Y; _ Time: ���- 'All or PM
# Hrs:
Grab
Date:
Time: ' AM or PM
Sample Volume:
Chilled during Collection?
Method of Transportation to the Lab -
&or No
E1T ID A 46030A
F c Analytical, Inc. Use Only -
Pace Work Order Numbe
r: ,� � W(
Receiving Temperature: (,
Received 13y: L'
Pace Analytiral Services, Inc. Address: 6701 Conference Dr., Raleigh, NC 27607 Phone: [phone]
Page 5 of 9

A
Chain of Custody �V'7
Workorder: 92274466 Workorder Name: BIOASSAY 11/03/2015
Report/ Invoice1To ,. = SubcontractTo`" "
77
Taylor Ezell .
Pace Analytical Charlotte P. 0:� 1 j 5�n
9800 Kincey Ave. Suite 100 < <
Huntersville, NC 28078
Phone [phone]
Email: [e-mail]
-.: Preserved Containers:;
Collect :,
Item Sample iD Date/Tim
e Lab:1D Matrixz
1 PIPE 001 11/3/2015 09:00 92274466001 Water
2
m
co
3
Cr 4
0
ca 15
--.AaceAnalytfcal o
My pacelabs.cam
i
Results Requested 11/24/2015
Requested Analysis ; -
V)
Transfers Releasep)1 1 Comments
Date/Time eceived By Daterrme
1
WAAA41ol A, Y
2 f-Ic.G., ' S
s
103v
Cooler Temperature on Receipt t).O °C Custod Seal Y or : N
Received on ice or N Samples Intac
***In order to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be provided on this COC document.
This chain of custody is considered complete as is since this information is available in the owner laboratory.
oGt'm
1 @
LAB USE ONLY
or N
Thursday, November O5, 201512:22:06 PM FMT ALL-C-002rev.00 2410arch2009
Psas 1 Of 1

Bioassay Chain of Custody Form
Facility Name: 0
Address: P.O. Box:
4) Air/ �i � ��� c�a 4- 3 r ly � ���,i. ,�,/�: �� Phone # : `��. 2
--N �- "� . ''�0' Contact: r zy �. �: 5,` C ev
County:___ NPDES permit #: a� ` `�. % pipe:
% Effluent Dilution (IWC): Plant Flow:
Select Test Method:
❑
C. dubla Pass/Fail Chronic
600CPFCNC
P.promelas Pass/Fail Acute 600A PRPpr
❑
C. dubla Full Range Chronic
600C FRCNC
❑ P.Promelas Full Range Acute 600A FRPpr
❑
C. dubla Pass/Fall Acute
600A PFCdu
❑ P.promelas Full Range Chronic 821C FRPpr
❑
M. bahla Pass/Fail Acute
600A PFMba
Sample Collector. Print _ 1
Signature
04
Sample Type:
Composite
Date Started
Time: 194S p 69or PM
Date Ended
- t- Time: Qt•Q r PM
Samples per Hr:
# Hrs: 1.%k�
Grab
Date:
Time: AM or PM
Sample Volume:
Chilled during Collection? �er No
Method of Transportation to the -Lab:
i`h�in of i`a �+nrly Qole.�'�.e .
Relin
Received By
A �
I,. =_r
M2 M
NOW-.
i1r,
115A Mul
__ -
MIN I a me,
Comments:
ft(po3o Q
TACE9
PAnalytical, Inc. Use Only -
Pace
Work Order Number: �
Receiving Temperature: Received By:
Pace Analytical Services, Inc. Address: 6701 Conference Dr., Ral'elgh, NC 27607 Phone: [phone]
09 a
Page 7 of 9

'o
9D
m
00
0
co
Chain Of Custody
Workorder: 92274466 Workorder Name:
BIOASSAY 11/03/2015
Results Requested
aceAnalxical
► WW-Pacelabs.com
11 /24/2015
"��C�v�u vn ice Y or N Samples Intact '
"#*In order to maintain client confidentiality, location/name of the sampling site, sampler's name and signature may not be rovide
This chain of custody is considered complete as is since this information is available in the owner laboratory. P don this COC document.
arnplt Colleciical dGt&I-b/'Yt2 WtiS <1110Jl5 of 9-00 cam-,.,
cSae' 0 f�'w coC.
Friday, November 06, 2015 1:40:19 PM
FMT ALL-C-002rev 00 24March2009
Page 1 of 1

"AWN,
Document Name: Date Revised: June 12.2015
_ Bioassay Chain of Custody Pago 1 of 1
�3�aAn. tf ICt7l Documanl Number Issuing Authodly:
F-RAL-CS-007-rov.02 Paco Carolinas Quality office
Bioassay Chairs of Custody Form
Y Facility Name: 2
Address: P.O. Box:
Phone # , _.(.� ' V-3
d---'�� Contact:
County : Del 4AA14 NPDES permit #: l'° a pipe:
% Effluent Dilution (IWC): Plant Flow:
Select Test Method -
❑ C. dubia Pass/Fail Chronic 600CPFCNC D P.promelas Pass/Fail Acute 600A PFPpr
❑ C. dubia Full Range Chronic 600C FRCNC ❑ P.promelas Full Range Acute 600A FRPpr
❑ C. dubia Pass/Fail Acute 600A PFCdu ❑ P.promelas Full Range Chronic 821C FRPpr
❑ M. bahia Pass/Fail Acute 600A PFMba '
-Sample Collector Print A� 6A_J'W 4'L b ' JIM
r Signature /�-�
Sam le Type:
Composite
Date Started .-' / Time: 'covio or PM
Date Ended Time: M r PM
Samples per Hr; _ # Hrs: C
Grab
Date: Time: AM or PM
Sample Volume; Chilled during Collection? Y s r No
Method of Transportat!on to the Lab:
('_hnin of (!i c•+nrly Delnne.r. .
Relinquished By
Date
Tlme
Received By
Date
Time
a
col..-Mef1cs:
For Pace Analytical, Inc. Use Only -
Pace Work Ordc;r Number:
ReceKeIng Temperature: Received By:'7
C� ---
--
Pace Analytical Services, Inc. Address: 6701 Conference Dr., Raleigh, NC 27607 Phone: [phone]
Page 9 of 9