NC0020800_Toxicity Test_20230705

RE: Town of Andrews WWTP NPDES Permit Renewal
Permit No. NCO020800
Ms. Thedford,
Attached you will find the fourth of our toxicity analysis for second species — Fathead Minnow,
Pimephales Promelas, parameter code THP6C — that was not included in our recent permit renewal
submission. We are also doing another in September if it is needed. Additionally I made a note on the
permit cover sheet explaining why we held off on submitting the permit due to this testing; please take
a look at that. We do not want anyone feeling as though we had dropped the ball or had had an
oversight. Thank you.
Timothy Wood
(828)-57-8630
[e-mail]
RECEIVED
JUL 05 202-3
NCDEO/DWR/NPDES

11
_. Emlronmema� r.:m,y sdunax, m�.
June 20, 2023
Ms. Wanda Ladd
Earth Environmental Services, Inc.
75 Bison Lane
Murphy, NC 28906
ETS Project Number: 17931
Test Start Date: June 06, 2023
Facility Tested: Andrews WWTP
PO Box 7565
Asheville, NC 28802
Phone: [phone]
Fax: [phone]
RECEIVED
JUL 0 5 2023
NCDEQ/DWR/NPDES
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Parameter
Code Test Procedure, Method Number Result (ChV)
THP6C Fathead minnow, Pimephales promelas, Larval Survival and Growth Chronic
EPA-821-R-02-013, Method 1000.0 >52%
All toxicity testing results required as part of your permit must be entered on the Effluent Discharge
Monitoring Form (MR-1) for the month in which it was performed, using the parameter code THP6C.
Additionally, the DMR Reporting Form AT-5 must be signed and e-mailed or mailed to the following
address:
ATForms.ATBa_ncdenr aov
North Carolina Division of Water Resources
Water Sciences Section / Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
Please call if you have any questions concerning these results.
Sincerely,
11 Sumner
Laboratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037, Drinking Water: 37786, Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001

PO Box 7565
Asheville, NC 28802
Phone: [phone]
tw a. «.arrswwswuoo�im Fax: [phone]
Effluent Toxicity Report Form - Chronic Fathead Minnow Multi -Concentration Test Date: June 20, 2023
Facility: Earth Environmental Services, Inc. NPDES #: NC 0020800 Pipe #: 001 County: Cherokee j
AndrewsWWTP
aboratay Per,..
-- - • ••••e ­ ' En vi,+,mental l esting solutions
c., Cerriticate # 037 Comments
iignature of Operator in Responsible Charge (ORC):
)RC Phone / E-mail:
Signature of Laboratory Supervisor: �
Project #: 17931
Sample if: 230605D1, 230607.26. 230609.12
e-Mail to: AT Forms.ATBColncdenr eov
Test Organisms: Pimephales promelos
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section / Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
Test Start Date: 1 June 06, 20,
Control
Organisms
% Effluent
3.25%
Replicate number
Supplier
In-house Culture
Begin hatch: 06-05-23 1201
End hatch: 06116-23 0500
Surviving number of larvae
10
10
10
10
Original number of larvae
1 10
1 10
10
10
Weight/onginal (mg/larvae)
1 0.878
1 0.7 77
0.662
0.777
Surviving number of larvae
10
10
10 10
Original number of larvae
l0
10
10 10
weight/original (mg/larvae)
0.841
0.753
0,698 0.748
% Effluent
Surviving number of larvae
1 10
1 10
1 10
10
6.5%
Original number of larvae
10
1 10
1 10
10
Weight/original (rrg,9arvae)
0.768
0.682
0.682
0.756
% Effluent
1 Surviving number of larvae
10
10
10
10
13%
Original number of larvae
Weight/onginal (mg/lariae)
10
10
10
10
0.822
U.774
0,849
0.752
% Effluent
26%
% Effluent
52%
Water Quality Data
Control
PH (Su):
DO (nWL):
Temp. (°C}:
High Concentr
PH (SU):
00 (rng/L):
Temp. (°C):
Surviving number of larvae
9
10
10
10
Original number of larvae
10
10
10
10
Weight/original (mg/larvae)
0.633
0.850
0.752
1 0.603
Surviving number of larvae
10
9
10
10
Original number of larvae
10
1 10
10
10
Weight/originai (mg/larvae) 1
0.712
1 0.735
0.759
0.758
survival (%)
100.0
Average wt(mg)
0.769
Average wt /
LO.764
surviving (mg)
Survival (%} 100.6
Average wt (mg) 6.760
Survival (%) 100.0
Average wt (mg) 0.722
Survival (%) 100.0
Average wt (mg} 0.749
Survival (%) 97.5
Average wt (mg) 0.710
Survival (%) 97.5
Average wt (mg) 0.741
Day 0
Intial Final
�y 1
initial Final
Day 2
Initial Finalt
Day 3
Initial Final
Day 4
Initial Final
Initia�ySFinal
InitiaDay6
=final
7.74
7.5
24.8
ttion
7.72
7.6
24.5
7.77
7.9
24.8
7.54
7.2
1 24.6
7.87
Z7
24.7
7.68
7.3
24.5 1
7.90
7.7
24.7
7,70
7.7
24.8
7.77
7.9
24.7
7.57
7.2
24.6
7.78
1 7.3
24.1
7.61
7.5 1
24.2
7.81
7.40
7.7
5.4
24.6
24.3
7.69
7.6
24.9
7.66
7.7
24.7
7.71
7.8
25.0
7.58
7.0
24,2
7.62
7,4
24.8
7.64 7.76 7.70 7.48
ZS 8.0 7.8 7.9
24.6 24.7 24.4 24.8
7.52
7.1
24.5
7.84
7.9
24.9
7.55 i.83 7.29
7.3 7.9 5.2
24.5 24J 24.6
Sample Information
Collection start dare:
Grab:
Composite duration:
Alkalinity (mg/L CaCo,):
Hardness (mVL CaCO,).
Conductivity (µmhos/cm):
Total residual chlorine (mg/L):
Sample Temp. at Receipt (°C):
Analyses
Normal:
Hom. Var-
NOEC:
LOEC:
ChV:
Yes
SurvivaV>52%
Yes
52%
>52%
>52%Method:
Visuallns
Overall Analysis:
Result: PASS LOEC: >52% NOEC: 52% ChV: >52%
DWR Report Form AT-5
%EfiNuent
V^Calculated
Critical
Calculated
Critical
3.2S%
2.410
0.173
6.5%
t.41C
0.948
13%
2.410
-0.627
26?6
2.410
1.202
52%
2.410
0.560

Page 1 of 5
EnWronmenta�Tecting Solutirn�s lrK.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimepha pr melas
Client: Andrews WWTP County: Cherokee
NPDES #: NC 0020800 Outfall #: 001
Project#: (1i 1
Dilution nrenarotinn
Dilution prep
3.25
6.5
13
26
52
Sample was not aerated or treated unless otherwise
noted on this form. Sample was warmed to 25.0 ± 1.o*c
in a warm water bath and then diluted to the test
concentrations with moderately hard synthetic water
(MHSW).
Effluent volume {ml)
(m
32.5
65
130
260
520
Diluent volume (ml.)
I
967.5
335
770
440
480
Total volume (mQ
TPO nrnnnie� i..fa..»...a:,.».
1000
1000
1000
10DO
1000
Organism source:
Age:
Spawn date:
Hatch dates and times:
Transfer vessel information:
Average transfer volume (m
+c�a rn7arrnauon:
In-house culture
Randomizing template:
< 24-hours old
Incubator number
and shelf location:
•
Artemio CHM number:
-
Ot.•04- vs
Drying information for wF
6-tI-Lo
(N.-01. tj OSOU
Date / Time in oven:
pH (S.U.) = t- t� -
_
`Initial oven temperature:
Date / Time out of oven:
Temperature (aC)
*Final oven temperature:
< 0.25 mL
Total drying time:
-% I->
CHM1222
determination:
60 't-
•14-� Ot�
t.a •L
— , --1, 1liermumeter ,N: 14-985135
Day
Date
Morning feeding
Afternoon feeding
Test initiation, renewal,
Sample
MHSW
or termination
number
batch used
Time
I Analyst
Time
T Analyst
Time
Analyst
used
0
06-06-23
OSO
t170
vtAs
�a+.os n t
us io•t�
1
06-07-23
(JS00
V1
11b0
CT110
.
2
3
06-08-23
o n
�Z"�o
(i�•�o
Z <01 u.
�•cb A
06-09-23
0 b
t1D
Cr1 bo
4
5
06-10-23
to l
11 t 0
1,3 11.-
OtrOS• tS
06.11-23
oks00
1'l00
6
06-12-23
06
tto
7
06-13-23
V�'1»
rh rn;enI
rrnwl.......•.
Parameter
t Reporting Limit
Method number
Meter
Serial number
PH
0.15.U.
SM 4500 H+ 8_2011
Accumet AR20
93312452
Dissolved Oxygen (D.O.)
1.0 mg/L
SM 4500-0 G-2016
YSI Model 52CE
180104324
Conductivity
14.9 µmhos/cm
SM 2510 B-2011
Accumet AR20
93312452
Alkalinity
5.0 mg CaCO3/L
SM 2320 B-2011
Accumet AR20
9331Z452
Hardness
5.0 mg CaCO3/L I
SM 2340 C-2011
Not applicable
Not applicable
Chlorine, Total Residual
0.1 mg/L
ORION 97-70-1977
Accumet AB250
92349123
Temperature
0.1 °C
SM 2550E-2010
Digital Thermometer
k3ab6,% IS
Control information.
% Mortality: Y
Average weight per initial larvae: o.16a
Average weight per surviving larvae: p.1 AL
cceptance criteria
<_ 20%
Summary of test
7-day LCso (%)
NOEC (%)
endpoints:
7' S /•
% .
>_ 0.25mg/larvae
LOEC (%)
ChV (%)
7 a f ,
? •f,
ICU (%)
7 5 a % •
SOP AT20-Revision 6-Exhibit AT20.3

Page 2 of 5
Environmental Testing Sdutions, 1M.
Species: Pimephales promelas
Client: Andrews WWTP
Date:
05-06-23
Survival
and Growth
Data
Day
CONTROL
3.25%
6.5%
A
B 1 C
7 D E
F I G
H 1
1 K
L
0
10
10 10
10 10
10 10
10 10
10 10
10
1
to
10 10
10 t0
tt3 tp
to ►b
rp 10
t0
2
t(}
1t} It)
10 t13
1p (0
!p /D
tp to
t0
3
It
to It
IO I
to Itj
►b 1p
10 10
/a
a
�U
(0 rb
tt) ro
10 to
1b tp
tp tp
1p
s
to
l0 10
10 to
to tp
to /0
10 10
/p
6
14
10 /o
i ►o t0
/0 la
t0 10
1p to
ro
to
10 to
Io It
(0 io
tp 16
10 to
to
*A = Pan weight (mg)
Tray color code: yill Ov✓
_
Analyst: __91•
Date: O S • 1 1 • 13
1$ 5l.
13.211 13.50
ISL 1 { I;j. wg
tit.t 3 )y l !
1V 70 )3. 2'1
1 y: `10 I3, 7!.
16.30
"B = Pan + Larvae weight (mg)
Analyst: 8 L
Date: 06.IS- Z 3,
22j4
P. Sr5Y0.1Z
21.9f 2.241
21 •GG 21.07
22.15' 20.97
Z1.22 2O.S9
1.39G
C =Larvae weight (mg) = B — A
Analyst:
Weight per initial number of larvae (mg)
= C / Initial number of larvae
,t
Analyst:
v
�►
^)
a� e6
N
v
v
_ y i
o
q' o•
CO
�
Ii? N,
o
� ^�o
b.
No
Average weight per initial Percent
o
v•
O o,
o,
number of larvae (mg) reduction
4
0 ,
O t . t
7. 0 .'t1
- (, . t
1.
control (%)
*Weight measurements performed using
Cahn
28 Automatic
Electrobalance,
SN 41520.
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small,
Ig = unusually large, d&r = decanted and returned, w = wounded.
SOP AT20-Revision 6—Exhibit AT20.3

Page 3 of 5
Envimnmcntal Testing Solutions, Inc.
Species: Pimephales promelas
Client: Andrews WWfP
S"ruiurrf nnif
Date: 06-06-23
Y W1V VL11 ✓ULLa
Day
13%
26%
52%
M
N
O
P
Q
R
S
T
U
V
W
X
0
10
10
10
10
10
10
10
10
10
10
10
10
1
to
to
r o
ro
r0
+D
+o
l o
+0
1Q
+ o
to
2
(
/�
10
rp
/p
la
at-
ro
tp
to
113
10
r0
to
3
1D
rp
tp
!p
°1
I(D
/0
10
r0
4011194
ra
10
a
10
to
lu
r0
l0
(0
tlb
!0
°!
10
Ij�
5
10
10
10
10
tp
1v
10
10
01
t0
r0
6
It)
r0
1b
to
°�
I(}
r0
!p
to
01
t0
10
7
Ip
1p
1D
i0
°t
10
10
16
1C3
113
10
*A = Pan weight (mg)
Tray color code: 11000 ✓
Analyst: 6 -
Date: 05 •21 •13
1l{. 5 5
15.80
R 75
i5. 05
15. 5 5
?. 2
1y
1. 01
15. 07
11• sa
13.13
* B = Pan + Larvae weight (mg)
Analyst: OL
Date: 0&- 15.23
22.77
23. SIt
23.2.4
22.57
2).
23.7+r
20.$3
21.10
21•G2
O.yg
22,39
20,
C = Larvae weight (mg) = B - A
B.'L't.
'1,14
Q.ag
-1.S2
6.16
8.so
-i.S2
b•(i3
�. tt
�S
1.s9
�.Sg
Analyst:
Weight per initial n-um'*ber of larvae (mg)
= C / Initial number of larvae
Analyst
rV ry
%~
��
Q'
N
b
n
M
�y0
nh
o'
Average weight per initial
Percent
number of larvae (mg)
reduction
©.'1tU
"1.�1 y%,
O -\L1 1
from control
N
*%Alo; k+
_r_
-.5 , ���caauicntcufl ta cl IVIItIeU USillP, l.dnrl 28 Automatic tiectrooalance, SN 41520.
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small,
Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:

F„�no,m..,nr i rr,.,y s,wuas inr
Quality Control: Verification of Data Entry, Calculations, and Statistical Analyses
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephales promelas
Facility: _Andrews WWTP
Test start date: June 6, 2023
Project number: 17931
Sample numbers 230605.01, 230607.26, 230609.12
ConcentrKbn(%I ( Replkat• lnafal num6ar of final numlwr
larvae
Control
'"
C
•"
10
lu _
10
132E
13.50
D
IV
1 10
14.11
3.25%
E
10
10
14A8
E
30
10
I4.13
C,
10
10
14,11
N
10
10
14.70
6.5%
I
10
10
13.29
1
30
10
lAAO
K
10
10
13.76
I
10
10
16.30
13%
M
10
10
14.55
N
10
10
15.80
D
10
10
14.75
P
10
10
15.05
26%
Q
10
9
15.55
R
30
10
1524
5
10
10
13.01
T
10
10
15.0/
52%
U
10
10
14.50
V
W
x
IO
10
9
10
13.13
14.80
=Panttarva•
wN[�Imf)
larval Wight (mg)
Wfghi/Surviving
Mean welglN/
C9efrKienTnf viY6lnen
Weight/Inhlal nui
aB-A
M1wb•rofarva•(m[i
5urviv1n4num6arof
W. .�anr.o ar. d+,
pflarvaelmg)
larvae {m[i
•r v....,1%{
23.34
8.78
0.878
20.85
7.57
0.757
20.12
6.62
0.662
D.769
11.5
0.757
21.8$
7.77
0.777
- 0,662
22.89
8.41W81,4
0.777
21.66
7.53
0.84/
6.980.
60
J.8022.28
0JS;i21.09
7.48
20.97
7.680.768
0,748
21.12
6.820.682
2058
6.82.5.40.682
23.86
7.56
22.77
8,22
0.822
0.756
2354
7.74
0.774
0,822
23.24
8.49
0.349
0.799
5.5
0.774 _
22.57
7.52
0.752
0, 849
21.88
6.33
0.703
0.752
23.74
8.50
0,850
0.633
20.53
7.52
0.752
0.727
14.1
0.633
50
21.10
6.03
0,603
0,7520,603
21.62
7.12
0.717
20.48
7.35
0.817
0.712
2239
co
,. per,
0.761
5_6
0.735
En4rc4 by
Kcecy [fClldll
RtrvkwcA by;
Jan su�Kr
can survival
I%(
Mean Wight)
Inatalnambof
ar
arv+• Im[I
r frKw ntofvariation
• i%)
P•rantr•duttion to
control (%{
100.0
0.769
Not applicable
100.0
0.760
7.8
1.1
100.0
0.722
6.4
6_
100.0
0.799
5.5
-4,0
97.5
0,710
16.0
7.7
97.5
0.741
30
10 3.6
10 12.48 20.06 7.58 0.758
0,758
Qutfall_001: MSD = Minimum Significant Difference
Dunnett's MSD value: 0.1183 PMSD = Percent Minimum Significant Difference
PMSD: 15.4 PMSD is a measure of test precision, The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whale effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 12%.
Upper PMSD bound determined by USEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2001a, 2001b. Final Report: interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-8-01-004 and EPA-821-B•01-D05. US Environmental Protection Agency, Cincinnati, CH.

Statistical Analyses
7i�luy Ne:r�ms
AtA��St{}Ujj�mjn.r
...,' EfrvilMilnC!I}8i iL5fdp4 $P11:h4n5InL
a Lrval Fish Growth and Survival Test-7 Da Growth
Start Date:
6/6/2023
Test ID:
PpFRCR Sample ID
Andrews
End Date:
Sample Date:
6/13/2023
Lab ID:
ETS-Envir Testing Sol. Sample Type:
DMR-Discharge Monitoring Report
Comments:
Protocol:
i=WCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas
Cone-%
1
2
3
4
D-Control
0.8780
0.7570
0.6620
0.7770
3.25
0 8410
0.7530
0.6980
0,7480
6.5
0.7680
0.6820
0.6820
0.7560
13
0.8220
07740
0.8490
0.7520
26
0.6330
08500
0.7520
0.6030
52
0.7120
0.7350
0.7590
0.7580
Cone %
Mean N-Mean
Mean
Transform: Untransformed
Min Max
1-Tailed
Isotonic
D-Control
0. 6685 1.0000
0.7685
Cv% N t-Stat
0.6620 0,8780 11,526 4
Critical
MSD
Mean N-Mean
3.25
07600
0.9889
0.7600
0.6980 0.8410 7.821 4 0 173
2.410
0.1183
0.7685 1,0000
0.7604
6.5
0.7220
09395
0.7220
0.6820 0.7680 6.433 4 0.948
2.410
0.1183
0.9895
0.7604 0.9895
13
0.7993
1.0400
0.7993
0.7520 08490 5531 4 -0.627
Z410
0.1183
0,7604 0.9895
26
52
0.7095
0.9232
0.7095
0.6030 08500 16.017 4 1.202
2.410
0.1183
0.7253 0.9437
0.7410
0,9642
0.7410
0.7120 0.7590 3 008 4 0,560
2.410
0.1183
0,7253 0.9437
Auxiliary Tests
ShapFro Witk's Test indicates normal distribution (p > 0.01)
Statistic
Critical
Skew Kurt
Bartlett's Test indicates equal variances (p = 0.19)
0.97876
7.47112
0.884
0 32746 0.17991
Hypothesis Test (1-tail, 0.05) NOEC LOEC Chv
TV MSDu MSDp
15.0863
MSB
MSE F Prob
Dunnett s Test 52 >52
Treatments vs D-Control
192308 0,11827 0,1539
0.0043
df
000482 0.50677 5, 18
Linear interpolation (200 Resamples)
Point % SD 95%CL Ex Skew
IC05 24 216
IC10 >52
IC15 >52
1,C
IC20
>52
IC25
>52
0.9
IC40
>52
0.8
IC50
>52
07
d
w
c 0.5
0
a 0.4
m
0.3
0.2
0.1
0.0
-0.1
Dose -Response Plot
1
09
0.8
0.7
c
0.0
O 0.5
a
00.4
0.3
0.2
0.1
0
O N m v ry
M
U
0
0 20 40 60
Dose %
1-tail. 0.05 level
of significance

Page 4 of 5
Environmental Testing Solutions, inc.
Species: pimephates prorne/as
Client: Andrews WWTP
Date: 06-06-23
Daily Chemistry.
Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table
located on Page 1. Alkalinity, hardness and chlorine (total residual) performed by the analyst identified on the bench sheet specific for each
analysis and transcribed to this bench sheet
Analyst
(Analyst identified
i3C
for each day,
L
performed
Day
pH, 0.0. and conductivity
1
measurements
only.)
2
3�
Concentration
Parameter
CONTROL,
MHSW
3.2590
PH (S.U.)
Dissolved oxygen (mg/L)
Conductivity (µmhos/cm)
'.7.17LY
-S_ S
3 f If
7. '7 7.77
3 t L►
G7 7. G7
•�
31111.
S
`
t
6V
—
7.G 7
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO3/L)
Temperature (°C)
PH(S.U.)
Dissolved oxygen (mg/L)
7.7-7
11.
Conductivity (µmhos/cm)
310
Temperature(°C)
PHIS.U.)
1`i•
7.?7
1'i-
7- 48
1`�•
?. G$
U{, j
1�(.i
tt{ `t
u
6.5/0
Dissolved oxygen (mg/L)
-1 �
, 6
3 t 3
,Q
Conductivity (µmhos/cm)
3 ora
7emPerature (°C)
1.>{.
��►NEW
-1
1Z . i
-\-T �
1`{•
1N • b
13tio
o
26/
529'0
100% 1
PH(S.U.)
Dissolved oxygen (mg/L)
Conductivity (µmhos/cm)
7. -1
—X "1
3 oa
?. 70
'0
-7.71
3l1
�,
`�.
�'
II j
7-G 7
1.7
7• G S
Temperature (°C)
PH (S.U.)
Dissolved oxygen (mg/L)
Conductivity (µmhos/cm)
' A -I
7.74P
- -
_
Q g
7_
�(0
_ Q
7-G G
,XS - O
3
Z
•� !
Temperature (°C}
PH (S.U.)
'L�•
7. &
ZS • O
7.7 1
7- loLf
Dissolved oxygen (mg/L)
• 1.
/
Conductivity(µmhos/cm)
2g7
`
30t} '
•� v
v{ d
Temperature (°C)
pH(S.U.)
ZA_S
7.&(
-m
Dissolved oxygen (mg/L)
Conductivity (µmhos/cm)
Alkalinity (mg CaCO3/1.)
Hardness (mg CaCO3/L)
Chlorine (mg/L)
Temperature (°C)
C-O. to
Initial F' I
tsd�
is�
lna Initial Final initial Final
SOP AT20-Revision 6—Exhibit AT20.3

Page 5 of 5
FAWImnmertAl Testing Solutions, Inc.
RECEIVED
JUL 0 5 2023
NCDEQ/DWR/NPDES
Species: Pimephales pramelas
Client: Andrews WWTP nnta. nc_nc_»
SOP AT20-Revision 6—Exhibit AT20.3

351 Repot Street
"� Asheville, NC 28801
'
�1 ° Phone: [phone]
r7
--- Fax: [phone]
Environmental Testing solutions, Inc.
Whole Effluent Toxicity Chain -of -Custody Form
Facility: Earth Environmental Services NPDES #: NCO020800 Pipe #: 001 County: Cherokee
Andrews WW1 P Purchase order. _ _
Species: Mmepbala;spromelase Effluent dilution: 3.2596.5, 13, 26, 52%
'rest type: Chronic Definitive Parameter code: THPW
Sample information: (to be completed by sample collector)
Composite sample: Sample location:
Start date:L lute r1
Time:? volume collected for testing: 2jj t r,
End date: `j Time: fD , rf l Number of containers filed for testing:
Number of samples per hour:4t3.*# �€ Method of transport to laboratory:
Chilled during collection? LG.�rI
Comments:
If chilled, specify temperature: _ r ��
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by Sample collector and facility personnel)
Syat"Ple collected by:
I Iry
�^`
�gnswrc
Da;c and time
Relinquished by:
Punt
�1[t6tnfY
�L L1O lima•
Relinquished by:
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by.
+„ Jrov3,— iNoQ�l►--
Pnna sip.-OW;t»„m
Yu � N�
Samples received in good condition?:
Y. In
Tricking number: 0 (-�
Comments:
Received by:
Fran sig..mro 7a,na nn,e
Received by:
Received at ETS bv:
102,
r �n;
Sample temperature up')" receipt at ETS (eCI:
a. l �-
Total residual chlorine upon receipt at ETS:
(DPD Presence/.Absence Indicator, MDL - 0 t 0 uIVI) n'u.x A.—
Project number:'' t✓r Sample number:(jj

Environmental Testing Solutions, Inc.
Whole Effluent T
Facility: Earth Environmental Services
Andrews W WTP
ity Chain -of -Custody Form
NPDES *: NCO020800 Pipe #: 001
Purchase order:
351 Depot Street
Asheville, NC 28801
Phone: [phone]
Fax: [phone]
County: Cherokee
Species: Pintephales promelas Effluent dilution: 3.25, 6.5, 13, 26, 52%
Test type: Chronic Definitive Parameter code: THP6C
Sample information: (to be completed by sample collector)
SI
Composite sample: Sample location: KeA+
Start date: (e -3 Time: _ e Am Volume collected for testing: a cs4w, 5
End date: Time:A Number of containers filled for testing:
Number of samples per hour: yx• 1 t Method of transport to laboratory: sty riP 1"
Chilled during collection? Le(�15 Comments:
If chilled, specify temperature:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 6.OaC upon receipt at the laboratory.
-----r----- -+ .,_....... ......ww. ,...v .a.,uny paauuuct)
sample collected by:
M
k
PiAtl �� [ha amt time
Relinquished by
Received
fby-
�A4A. Cts
�tt5 M � 3
Riot Ymabm !�tr anJ liaa P—
Sample receipt information: (to be completed by ETS personnel)
RelinauishM to F.TC h.-
ti�ti>, ct fj
4 7
Custody seals intact?. Q Q
F'es tvo !Jot »ni
Samples received in good condition?:
Tracking number: tJ
Comments:
Received at FTC hv.
-T �L V, 0 �3
Sample temperattn'e upon receipt at ETS (aC):
Total residual chlorine upon receipt at E7•S:
(DPD PmsenceiAbstensc indicator, MDL =0.10 myL)
Project number- 1 Sample number: ft)00 4-2JO

. s
sue;
3,I .-
4
Environmental Testing Solutlons, Inc.
Whole Effluent
Chain -of -Custody Form
351 Depot Street
Asheville, NC 28801
Phone: [phone]
Pax: [phone]
Facility: Earth Environmental Services NPDES #: NCO020800 pipe #: 001 County: Cherokee
Andrews wWTp Purchase order:
Species: AMephates promelos Effluent dilution: 3.25, 6.5,13, 26, 52%
Test type: Chronic Definitive
parameter code: THp6C
Sample information: (to be completed by sample collector)
Composite sample: Sample location.
Start date. Time: tM Ail Volume collected for testing: pZ (Seim
End date: _
Time: M Number of containers filled for testing:
Number of samples per hour: /t} Method of transport to laborato
Chilled during collection? ry: —hurt(
If chilled, specify temperature: ! C Comments:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be < 60C. upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facilit
y personnel)
Sampie collected by:
mar-1." e
28r-lq
*,
a a-
2
i4un
Sigrunue
L7ate ud cme
Relinquished by-
martt� e.
(, �
5-0
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by:
f ..If
Nvr >Se 1k and tune
Custody seals mtuct'.:
No
Samples received in good condition': r1i Q
Tracking number:
Comments:
_PIT.,u,
ij
Received at ETS by:
LLB- E-7 3
Sample temperature opal receipt at ETS (°C): PEI
Total residual chlorine upon receipt at ETS:
i,DPD Presrnce/Ab-erase indicator, Nfl)L - 0.10 FnWt)
Project number: N31 Sample number: lob .

Ar Pomephales promelas
Chronic Reference Toxicant Control Chart
— Environment Tesung solutions, Inc. Source: In—house Culture
Control Limits (± 2 Standard Deviations)
r•
•
0.7 • i •
•
0.6
•
1.L
1.0
M
•
f
Warning Limits
12� p1 p"' p2 pb p3'pb �A GS �gA3O�.O� p� 11= Q,�,1�%O�A'L' �9.13' iQ OCr 110g 1,LA6 o1lp O�.O� D3 O"1 � OA ��A7. �bp6-1-3
Test date
• 7-day IC2s = 25% inhibition concentration. An estimation of the potassium chloride concentration
which would cause a 25% reduction in Pirrnepholes growth (calculated using ToxCalc),
�» Central Tendency (mean logarithmic IC 2. converted to anti -logarithmic values)
- Control Limits (mean logarithmic IC,, ± 2 standard deviations converted to anti -logarithmic values)
--- • • - - Laboratory Warning Limits (mean logarithmic IC25 ± 2 coefi•icent of variations converted to anti -logarithmic values)
•> ... USEPA Warning Limits (mean logarithmic IC,5 ± SA 75 converted to anti -logarithmic values,
SA.75 = 75`h percentile of CVs reported nationally by USEPA)
101"'.0
Revre.wa by
tvn wmn.r