NC0020800_Renewal (Application)_20230629

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ROY COOPER
Governor J �c
Q uArA
ELIZABETH S.BISER �Y«,,,M�,.
Secretary
RICHARD E.ROGERS,JR. NORTH CAROLINA
Director Environmental Quality
June 29, 2023
Town of Andrews
Attn: James Reid, Mayor
PO Box 1210
Andrews, NC 28901
Subject: Permit Renewal
Application No. NC0020800
Andrews WWTP
Cherokee County
Dear Applicant:
The Water Quality Permitting Section acknowledges the June 29, 2023, receipt of your permit renewal application and
supporting documentation. Your application will be assigned to a permit writer within the Section's NPDES WW permitting
branch. Per G.S. 150B-3 your current permit does not expire until permit decision on the application is made.
Continuation of the current permit is contingent on timely and sufficient application for renewal of the current permit. The
permit writer will contact you if additional information is required to complete your permit renewal. Please respond in a
timely manner to requests for additional information necessary to allow a complete review of the application and renewal
of the permit.
Information regarding the status of your renewal application can be found online using the Department of Environmental
Quality's Environmental Application Tracker at:
https://dea.nc.gov/permits-regulations/permit-guidance/environmental-application-tracker
If you have any additional questions about the permit, please contact the primary reviewer of the application using the
links available within the Application Tracker.
Sincerely,
eo��
Wren Thedford
Administrative Assistant
Water Quality Permitting Section
ec: WQPS Laserfiche File w/application
E C/s) North Carolina Department of Environmental Quality I Division of Water Resources
Asheville Regional Office 12090 U.S.Highway 70 I Swannanoa.North Carolina 28778
��+ �� / 828.296.4500

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2/24/23
NPDES PERMIT RENEWAL APPLICATION FOR ANDREWS, NC WASTEWATER TREATMENT PLANT
PERMIT#: NC0020800
Facility Address:
464 Reagan Ave.
Andrews, NC. 28901
Included: RECEIVED
• NPDES Renewal Application Form 2A .;uN 2 9 2023
• All relevant testing data
NCDEQIDWRINPDES
Attached Documentation:
• Pollutant Scan Analysis Data for Part B, Section B.6, and also Part D
o Annual Pollutant Scan for December 2019
o Annual Pollutant Scan for April 2020
o Annual Pollutant Scan for September 2021
o Annual Pollutant Scan for December 2022
• Complete Chronic Toxicity/Bioassay Data for Part E(Two Species)
o North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure (TGP3B) for
September, 2019
o North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure (TGP3B) for December,
2020
o North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure (TGP3B)for March,
2021
o North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure (TGP3B)for June, 2022
o Fathead Minnow, Pimephales Promelas, Larval Survival and Growth Chronic (THP6C)for
October, 2022
o Fathead Minnow, Pimephales Prornelas, Larval Survival and Growth Chronic (THP6C)for
December, 2022
o Fathead Minnow, Pimephales Promelas, Larval Survival and Growth Chronic (THP6C)for
March, 2023
o Last of yearly quarterly testing results for June 2023 to be submitted once we receive
them from laboratory.
Note: Last of yearly testing for June, 2023 for Fathead Minnow not received due to our facility only
being notified of having to test for it as of mid 2022. Up until then we were not required to test for this
species, so it has taken us this time to get all four of the test required completed.This was our reasoning
for previously waiting to submit our application.
• Topographic Map
• Basic Process Flow Diagram

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL i HIWASSEE
FORM
2A NPDES FORM 2A APPLICATION OVERVIEW
NPDES
APPLICATION OVERVIEW
Form 2A has been developed in a modular format and consists of a "Basic Application Information" packet
and a "Supplemental Application Information" packet. The Basic Application Information packet is divided
into two parts. All applicants must complete Parts A and C. Applicants with a design flow greater than or
equal to 0.1 mgd must also complete Part B. Some applicants must also complete the Supplemental
Application Information packet. The following items explain which parts of Form 2A you must complete.
BASIC APPLICATION INFORMATION:
A. Basic Application Information for all Applicants. All applicants must complete questions A.1 through A.8. A treatment works
that discharges effluent to surface waters of the United States must also answer questions A.9 through A.12.
B. Additional Application Information for Applicants with a Design Flow>_0.1 mgd. All treatment works that have design flows
greater than or equal to 0.1 million gallons per day must complete questions B.1 through B.6.
C. Certification. All applicants must complete Part C(Certification).
SUPPLEMENTAL APPLICATION INFORMATION:
D. Expanded Effluent Testing Data. A treatment works that discharges effluent to surface waters of the United States and meets
one or more of the following criteria must complete Part D(Expanded Effluent Testing Data):
1. Has a design flow rate greater than or equal to 1 mgd,
2. Is required to have a pretreatment program(or has one in place), or
3. Is otherwise required by the permitting authority to provide the information.
E. Toxicity Testing Data. A treatment works that meets one or more of the following criteria must complete Part E(Toxicity Testing
Data):
1. Has a design flow rate greater than or equal to 1 mgd,
2. Is required to have a pretreatment program(or has one in place), or
3. Is otherwise required by the permitting authority to submit results of toxicity testing.
F. Industrial User Discharges and RCRA/CERCLA Wastes. A treatment works that accepts process wastewater from any
significant industrial users(Sills)or receives RCRA or CERCLA wastes must complete Part F(Industrial User Discharges
and RCRA/CERCLA Wastes). SIUs are defined as:
1. All industrial users subject to Categorical Pretreatment Standards under 40 Code of Federal Regulations(CFR)403.6 and
40 CFR Chapter I, Subchapter N (see instructions); and
2. Any other industrial user that:
a. Discharges an average of 25,000 gallons per day or more of process wastewater to the treatment works(with certain
exclusions); or
b. Contributes a process wastestream that makes up 5 percent or more of the average dry weather hydraulic or organic
capacity of the treatment plant; or
c. Is designated as an SIU by the control authority.
G. Combined Sewer Systems. A treatment works that has a combined sewer system must complete Part G(Combined Sewer
Systems).
ALL APPLICANTS MUST COMPLETE PART C (CERTIFICATION)
FPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 1 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
BASIC APPLICATION INFORMATION
PART A. BASIC APPLICATION INFORMATION FOR ALL APPLICANTS:
All treatment works must complete questions A.1 through A.8 of this Basic Application Information Packet.
A.1. Facility Information.
Facility Name ANDREWS WASTEWATER TREATMENT PLANT
Mailing Address PO BOX 1210
ANDREWS,NC 28901
Contact Person James Reid TIM WOOD
Title MAYOR WWTP ORC
Telephone Number [phone] [phone]
Facility Address 464 REAGAN AVE.
(not P.O.Box) ANDREWS,NC 28901
A.2. Applicant Information. If the applicant is different from the above,provide the following:
Applicant Name TOWN OF ANDREWS
Mailing Address PO BOX 1210 _
ANDREWS,NC 28901
Contact Person James Reid
Title MAYOR
Telephone Number [phone]
Is the applicant the owner or operator(or both)of the treatment works?
(Si owner ❑ operator
Indicate whether correspondence regarding this permit should be directed to the facility or the applicant.
❑ facility ® applicant
A.3. Existing Environmental Permits. Provide the permit number of any existing environmental permits that have been issued to the treatment works
(include state-issued permits).
NPDES NC0020800 PSD
UIC Other
RCRA Other
A.4. Collection System Information. Provide information on municipalities and areas served by the facility. Provide the name and population of each
entity and,if known,provide information on the type of collection system(combined vs.separate)and its ownership(municipal,private,etc.).
Name Population Served Type of Collection System Ownership
Town of Andrews 1800 Separate _ Municipal
Total population served
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 2 of 22
I

FACILITY NAME AND PERMIT NUMBER: I PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
A.5. Indian Country.
a. Is the treatment works located in Indian Country'?
❑ Yes ® No
b. Does the treatment works discharge to a receiving water that is either in Indian Country or that is upstream from(and eventually flows
through)Indian Country?
❑ Yes ® No
A.6. Flow. Indicate the design flow rate of the treatment plant(i.e.,the wastewater flow rate that the plant was built to handle). Also provide the
average daily flow rate and maximum daily flow rate for each of the last three years. Each year's data must be based on a 12-month time period
with the 12th month of"this year"occurring no more than three months prior to this application submittal.
a. Design flow rate 1.5 mgd
Two Years Arlo Last Year This Year
b. Annual average daily flow rate 0.814(2020) 0.692(2021) 0.682(2022)
c. Maximum daily flow rate 3.430(3/25/20) 3.532(3/18/21) 3.532(2/04/22)
A.7. Collection System. Indicate the type(s)of collection system(s)used by the treatment plant. Check all that apply. Also estimate the percent
contribution(by miles)of each.
® Separate sanitary sewer 100
0 Combined storm and sanitary sewer
A.8. Discharges and Other Disposal Methods.
a. Does the treatment works discharge effluent to waters of the U.S.? ® Yes 0 No
If yes,list how many of each of the following types of discharge points the treatment works uses:
i. Discharges of treated effluent 1
ii. Discharges of untreated or partially treated effluent None
iii. Combined sewer overflow points N/A
iv. Constructed emergency overflows(prior to the headworks) N/A
v. Other N/A
b. Does the treatment works discharge effluent to basins,ponds,or other surface impoundments
that do not have outlets for discharge to waters of the U.S.? 0 Yes ® No
If yes,provide the following for each surface impoundment:
Location:
Annual average daily volume discharge to surface impoundment(s) mgd
Is discharge ❑ continuous or 0 intermittent?
c. Does the treatment works land-apply treated wastewater? 0 Yes ® No
If yes,provide the following for each land application site:
Location:
Number of acres:
Annual average daily volume applied to site: mgd
Is land application 0 continuous or ❑ intermittent?
d. Does the treatment works discharge or transport treated or untreated wastewater to another
treatment works? 0 Yes ® No
EPA Form 3510-2A(Rev. 1-99). Replaces EPA forms 7550-6&7550-22. Page 3 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
If yes,describe the mean(s)by which the wastewater from the treatment works is discharged or transported to the other treatment works
(e.g.,tank truck,pipe).
If transport is by a party other than the applicant,provide:
Transporter Name
Mailing Address
Contact Person
Title
Telephone Number ( )
For each treatment works that receives this discharge,provide the following:
Name
Mailing Address
Contact Person
Title
Telephone Number (
If known,provide the NPDES permit number of the treatment works that receives this discharge
Provide the average daily flow rate from the treatment works into the receiving facility. mgd
e. Does the treatment works discharge or dispose of its wastewater in a manner not included
in A.8.through A.8.d above(e.g.,underground percolation,well injection): ❑ Yes ® No
If yes,provide the following for each disposal method:
Description of method(including location and size of site(s)if applicable):
Annual daily volume disposed by this method:
Is disposal through this method ❑ continuous or ❑ intermittent?
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 4 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
WASTEWATER DISCHARGES:
If you answered"Yes"to question A.8.a,complete questions A.9 through A.12 once for each outfall(including bypass points)through
which effluent is discharged. Do not include information on combined sewer overflows in this section. If you answered"No"to question
A.8.a,go to Part B,"Additional Application Information for Applicants with a Design Flow Greater than or Equal to 0.1 mgd."
A.9. Description of Outfall.
a. Outfall number 001
b. Location VALLEY RIVER,ANDREWS,NC. 28901
(City or town,if applicable) (Zip Code)
CHEROKEE NC
(County) (State)
35°11'51' 83°50'46
(Latitude) (Longitude)
c. Distance from shore(if applicable) N/A ft.
d. Depth below surface(if applicable) N/A ft.
e. Average daily flow rate 0.633 mgd
f. Does this outfall have either an intermittent or a periodic discharge? ❑ Yes ® No (go to A.9.g.)
If yes,provide the following information:
Number f times per year discharge occurs:
Average duration of each discharge:
Average flow per discharge: mgd
Months in which discharge occurs:
g. Is outfall equipped with a diffuser? ❑ Yes ® No
A.10. Description of Receiving Waters.
a. Name of receiving water VALLEY RIVER
b. Name of watershed(if known) HIWASSEE RIVER WATERSHED
United States Soil Conservation Service 14-digit watershed code(if known):
c. Name of State Management/River Basin(if known):
United States Geological Survey 8-digit hydrologic cataloging unit code(if known):
d. Critical low flow of receiving stream(if applicable)
acute cfs chronic cfs
e. Total hardness of receiving stream at critical low flow(if applicable): mg/I of CaCO3
EPA Form 3510-2A(Rev. 1-99). Replaces EPA forms 7550-6&7550-22. Page 5 of 22

•
FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
A.11. Description of Treatment
a. What level of treatment are provided? Check all that apply.
® Primary ® Secondary
❑ Advanced ❑ Other. Describe:
b. Indicate the following removal rates(as applicable):
Design BOD5 removal or Design CBOD5 removal 85 _
Design SS removal 85 94.
Design P removal
Design N removal
Other
c. What type of disinfection is used for the effluent from this outfall? If disinfection varies by season,please describe:
CHLORINATION
If disinfection is by chlorination is dechlorination used for this outfall? ® Yes ❑ No
Does the treatment plant have post aeration? ❑ Yes ® No
A.12. Effluent Testing Information. All Applicants that discharge to waters of the US must provide effluent testing data for the following
parameters. Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is
discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data
collected through analysis conducted using 40 CFR Part 136 methods. In addition,this data must comply with QA/QC requirements of
40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a
minimum,effluent testing data must be based on at least three samples and must be no more than four and one-half years apart.
Outfall number: 001
MAXIMUM DAILY VALUE AVERAGE DAILY VALUE
PARAMETER
Value Units Value Units Number of Samples
pH(Minimum) 5.8 s.u.
pH(Maximum) 6.9 s.u. ,iS G < l
Flow Rate 3.498 MGD 0.729 MGD 1095
Temperature(Winter) 15.3 °C 12.5 °C 92
Temperature(Summer) 25.7 °C 22.5 °C 92
r
*For pH please report a minimum and a maximum daily value
MAXIMUM DAILY AVERAGE DAILY DISCHARGE
POLLUTANT _ DISCHARGE ANALYTICAL MUMDL
Number of METHOD
Conc. Units Conc. Units
Samples
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
BIOCHEMICAL OXYGEN BOD5 36.9 MG/L 20.7 MG/L 1460 SM5210B_ 2.0
DEMAND(Report one) CBOD5
FECAL COLIFORM 6800 #/100ML 110.6 #/100ML 1460 SM9222D 1/100m1
TOTAL SUSPENDED SOLIDS(TSS) 55,5 MG/L 25.9 MGM_ 1460 SM25400 5.0
END OF PART A.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 6 of 22

•
FACILITY NAME AND PERMIT NUMBER: I PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
BASIC APPLICATION INFORMATION
PART B. ADDITIONAL APPLICATION INFORMATION FOR APPLICANTS WITH A DESIGN FLOW GREATER THAN OR
EQUAL TO 0.1 MGD(100,000 gallons per day).
All applicants with a design flow rate>-0.1 mgd must answer questions B.1 through B.6. All others go to Part C(Certification).
B.1. Inflow and Infiltration. Estimate the average number of gallons per day that flow into the treatment works from inflow and/or infiltration.
100,000 gpd
Briefly explain any steps underway or planned to minimize inflow and infiltration.
Ongoing identification and repair of problem areas by Sewer Collection Dept.
B.2. Topographic Map. Attach to this application a topographic map of the area extending at least one mile beyond facility property boundaries. This
map must show the outline of the facility and the following information. (You may submit more than one map if one map does not show the entire
area.)
a. The area surrounding the treatment plant,including all unit processes.
b. The major pipes or other structures through which wastewater enters the treatment works and the pipes or other structures through which
treated wastewater is discharged from the treatment plant. Include outfalls from bypass piping,if applicable.
c. Each well where wastewater from the treatment plant is injected underground.
d. Wells,springs,other surface water bodies,and drinking water wells that are: 1)within'A mile of the property boundaries of the treatment
works,and 2)listed in public record or otherwise known to the applicant.
e. Any areas where the sewage sludge produced by the treatment works is stored,treated,or disposed.
f. If the treatment works receives waste that is classified as hazardous under the Resource Conservation and Recovery Act(RCRA)by truck,rail,
or special pipe,show on the map where the hazardous waste enters the treatment works and where it is treated,stored,and/or disposed.
B.3. Process Flow Diagram or Schematic. Provide a diagram showing the processes of the treatment plant,including all bypass piping and all
backup power sources or redunancy in the system. Also provide a water balance showing all treatment units,including disinfection(e.g.,
chlorination and dechlorination). The water balance must show daily average flow rates at influent and discharge points and approximate daily flow
rates between treatment units. Include a brief narrative description of the diagram.
B.4. Operation/Maintenance Performed by Contractor(s).
Are any operational or maintenance aspects(related to wastewater treatment and effluent quality)of the treatment works the responsibility of a
contractor? ® Yes 0 No
If yes,list the name,address,telephone number,and status of each contractor and describe the contractors responsibilities(attach additional
pages if necessary).
Name: Earth Environmental Services
Mailing Address: 75 Bison Ln.
Murphy, NC. 28906
Telephone Number: [phone]
Responsibilities of Contractor: Laboratory Analysis/Sampling
B.5. Scheduled improvements and Schedules of Implementation. Provide information on any uncompleted implementation schedule or
uncompleted plans for improvements that will affect the wastewater treatment,effluent quality,or design capacity of the treatment works. If the
treatment works has several different implementation schedules or is planning several improvements,submit separate responses to question B.5
for each. (If none,go to question B.6.)
a. List the outfall number(assigned in question A.9)for each outfall that is covered by this implementation schedule.
001
b. Indicate whether the planned improvements or implementation schedule are required by local,State,or Federal agencies.
❑ Yes ® No
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 7 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
c. If the answer to B.5.b is"Yes,"briefly describe,including new maximum daily inflow rate(if applicable).
N/A
d. Provide dates imposed by any compliance schedule or any actual dates of completion for the implementation steps listed below,as
applicable. For improvements planned independently of local,State,or Federal agencies,indicate planned or actual completion dates,as
applicable. Indicate dates as accurately as possible.
Schedule Actual Completion
Implementation Stage MM/DD/YYYY MM/DD/YYYY
-Begin Construction (Tentative Date) / /
-End Construction (Tentative Date) / /
-Begin Discharge / / / /
-Attain Operational Level / / / /
e. Have appropriate permits/clearances concerning other Federal/State requirements been obtained? ® Yes ❑ No
Describe briefly:
B.6. EFFLUENT TESTING DATA(GREATER THAN 0.1 MGD ONLY).
Applicants that discharge to waters of the US must provide effluent testing data for the following parameters. Provide the indicated
effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include information
on combine sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition,this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate
QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a minimum effluent testing data must be
based on at least three pollutant scans and must be no more than four and on-half years old.
Outfall Number: 001 j
MAXIMUM DAILY 1
AVERAGE DAILY DISCHARGE
DISCHARGE ANALYTICAL
POLLUTANT ML/MDL
Number of METHOD
Conc. Units Conc. 1, Units Samples
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
AMMONIA(as N)
CHLORINE(TOTAL
RESIDUAL,TRC)
DISSOLVED OXYGEN
TOTAL KJELDAHL
NITROGEN(TKN)
NITRATE PLUS NITRITE
NITROGEN
OIL and GREASE
PHOSPHORUS(Total)
TOTAL DISSOLVED SOLIDS
(TDS)
OTHER
1 END OF PART B,
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE �� e ,, , i ,, I
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 8 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
BASICRAPP:LlCA ION ! RMATIO
PART C. CERTIFICATION
All applicants must complete the Certification Section. Refer to instructions to determine who is an officer for the purposes of this
certification. All applicants must complete all applicable sections of Form 2A,as explained in the Application Overview. Indicate below which
parts of Form 2A you have completed and are submitting. By signing this certification statement,applicants confirm that they have reviewed
Form 2A and have completed all sections that apply to the facility for which this application is submitted.
Indicate which parts of Form 2A you have completed and are submitting:
® Basic Application Information packet Supplemental Application Information packet:
® Part D(Expanded Effluent Testing Data)
® Part E(Toxicity Testing: Biomonitoring Data)
❑ Part F(Industrial User Discharges and RCRA/CERCLA Wastes)
0 Part G(Combined Sewer Systems)
r-z�-
ALL APPLICANTS MUST COMPLETE THE FOLLOWING CERTIFICATION.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system
designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who
manage the system or those persons directly responsible for gathering the information,the information is,to the best of my knowledge and belief,true,
accurate,and complete. I am aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment
for knowing violations.
Name and official title Ma or James Reid
ILI
Signature .� `
Telephone number (82 321-3113
Date signed
Upon request of the permitting authority,you must submit any other information necessary to assure wastewater treatment practices at the treatment
works or identify appropriate permitting requirements.
SEND COMPLETED FORMS TO:
NCDENR/ DWR
Attn: NPDES Unit
1617 Mail Service Center
Raleigh, North Carolina 27699-1617
EPA Form 3510-2A(Rev. 1-99). Replaces EPA forms 7550-6&7550-22. Page 9 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
•
SUPPLEMENTAL APPLICATION INFORMATION
PART D. EXPANDED EFFLUENT TESTING DATA
Refer to the directions on the cover page to determine whether this section applies to the treatment works.
Effluent Testing: 1.0 mgd and Pretreatment Works. If the treatment works has a design flow greater than or equal to 1.0 mgd or it has(or is required
to have)a pretreatment program,or is otherwise required by the permitting authority to provide the data,then provide effluent testing data for the following
pollutants. Provide the indicated effluent testing information and any other information required by the permitting authority for each outfall through which
effluent is discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected
through analyses conducted using 40 CFR Part 136 methods. In addition,these data must comply with QA/QC requirements of 40 CFR Part 136 and
other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. Indicate in the blank rows provided below
any data you may have on pollutants not specifically listed in this form. At a minimum,effluent testing data must be based on at least three pollutant
scans and must be no more than four and one-half years old.
Outfall number 001 (Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE j AVERAGE DAILY DISCHARGE
ANALYTICAL
Number{ POLLUTANT METHOD MUMDL
Conc. Units Mass Units Conc. Units Mass Units of
Samples t
METALS(TOTAL RECOVERABLE),CYANIDE,PHENOLS,AND HARDNESS.
ANTIMONY
ARSENIC 0.010 Mg/I 0.010 Mgil 3
BERYLLIUM
CADMIUM 0.001 Mg/I 0.001 Mg/I 3
CHROMIUM 0.005 Mg/I 0.005 Mg/I 3
COPPER 0.067 Mg/I 0.033 Mg/I 3
LEAD 0.005 Mg/I 0.005 Mg/I 3
MERCURY 0.0002 Mg/I 0.0002 Mg/I 3
NICKEL 0.010 Mg/I 0.010 Mg/I 3
SELENIUM 0.010 Mg/I 0.010 Mg/I 3
SILVER 0.005 Mg/I 0.005 Mg/I 3
THALLIUM
ZINC 0.080 Mg/I 0.044 Mg/I 3
CYANIDE 0.042 Mg/I 0.010 Mg/I 3
TOTAL PHENOLIC
COMPOUNDS
HARDNESS(as CaCO3) 72 Mg/I 65.2 Mg/I
Use this space(or a separate sheet)to provide information on other metals requested by the permit writer
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 10 of 22

•
FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE r
POLLUTANT i , 1 Number ANALYTICAL ML/MDL
Conc. Units Mass Units Conc. Units Mass Units of METHOD
Samples
VOLATILE ORGANIC COMPOUNDS
ACROLEIN
ACRYLONITRILE
BENZENE
BROMOFORM
CARBON
TETRACHLORIDE
CHLOROBENZENE
CHLORODIBROMO-
METHANE
CHLOROETHANE
2-CHLOROETHYLVINYL
ETHER
CHLOROFORM
DICHLOROBROMO-
METHANE
1,1-DICHLOROETHANE
1,2-DICHLOROETHANE
TRANS-I,2-DICHLORO-
ETHYLENE
1,1-DICHLORO-
ETHYLENE
1,2-DICHLOROPROPANE
1,3-DICHLORO-
PROPYLENE
ETHYLBENZENE
METHYL BROMIDE
METHYL CHLORIDE
METHYLENE CHLORIDE
1,1,2,2-TETRA-
CHLOROETHANE
TETRACHLORO-
ETHYLENE
TOLUENE
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 11 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, N0002O8O0 RENEWAL HIWASSEE
Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE
POLLUTANT I I Number ANALYTICAL ML/MDL
Cone. Units Mass Units Cone. Units Mass Units of METHOD
— I Samples
1,1,1-
TRICHLOROETHANE
1,1,2-
TRICHLOROETHANE
TRICHLOROETHYLENE
VINYL CHLORIDE
I Use this space(or a separate sheet)to provide information on other volatile organic compounds requested by the permit writer
ACID-EXTRACTABLE COMPOUNDS
P-CHLORO-M-CRESOL
2-CHLOROPHENOL
2,4-DICHLOROPHENOL
2,4-DIMETHYLPHENOL
4,6-DIN ITRO-O-CRESOL
2,4-DINITROPHENOL
2-NITROPHENOL
4-NITROPHENOL
PENTACHLOROPHENOL
PHENOL
2,4,6-
TRICHLOROPHENOL
Use this space(or a separate sheet)to provide information on other acid-extractable compounds requested by the permit writer
BASE-NEUTRAL COMPOUNDS
ACENAPHTHENE
ACENAPHTHYLENE
ANTHRACENE
BENZIDINE
BENZO(A)ANTHRACENE
BENZO(A)PYRENE
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 12 of 22

FACILITY NAME AND PERMIT NUMBER: I PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
)utfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE
ANALYTICAL
Number
POLLUTANT METHOD ML/MDL
Conc. Units Mass Units Conc. Units Mass Units of
Samples
3,4 BENZO-
FLUORANTHENE
BENZO(GHI)PERYLENE
BENZO(K)
FLUORANTHENE
BIS(2-CHLOROETHOXY)
METHANE
BIS(2-CHLOROETHYL)-
ETHER
BIS(2-CHLOROISO-
PROPYL)ETHER
BIS(2-ETHYLHEXYL) 10.1 Ug/1 8.38 Ug/I
PHTHALATE
4-BROMOPHENYL
PHENYL ETHER
BUTYL BENZYL
PHTHALATE
2-CHLORO-
NAPHTHALENE
4-CHLORPHENYL
PHENYL ETHER
CHRYSENE
DI-N-BUTYL PHTHALATE
DI-N-OCTYL PHTHALATE
DIBENZO(A,H)
ANTHRACENE
1.2-DICHLOROBENZENE
1,3-DICHLOROBENZENE
1,4-DICHLOROBENZENE
3,3-DICHLORO-
BENZIDINE
DIETHYL PHTHALATE
DIMETHYL PHTHALATE
2,4-DINITROTOLUENE
2,6-DINITROTOLUENE
1,2-DIPHENYL-
HYDRAZINE
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 13 of 22

•
FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
Outfall number r)01 (Complete once for eacn outfall discharging effluent to waters of the United States.)
MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE
POLLUTANT Number ANALYTICAL
Conc. Units Mass Units Conc. Units Mass Units of METHOD
Samples
FLUORANTHENE
FLUORENE
HEXACHLOROBENZENE
HEXACHLORO-
BUTADIENE
HEXACHLOROCYCLO-
PENTADIENE
HEXACHLOROETHANE
INDENO(1,2,3-CD)
PYRENE
ISOPHORONE
NAPHTHALENE
NITROBENZENE
N-NITROSODI-N-
PROPYLAMINE
N-NITROSODI-
METHYLAMINE
N-N ITROSO D I-
PHENYLAMINE
PHENANTHRENE
PYRENE
1,2,4-
TR ICHLOROBENZENE
Use this space(or a separate sheet)to provide information on other base-neutral compounds requested by the permit writer
l I
Use this space(or a separate sheet)to provide information on other pollutants(e.g.,pesticides)requested by the permit writer
i 1
. s,..,:, END OF PART D.
REFER TO THE APPLICATION OVERVIEW(PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 14 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
1
SUPPLEMENTAL APPLICATIOM ORI +t
PA E. rGx TEs II y K - 1
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points: 1)POTWs with a design flow rate greater than or equal to 1.0 mgd;2)POTWs with a pretreatment program(or those that are
required to have one under 40 CFR Part 403);or 3)POTWs required by the permitting authority to submit data for these parameters.
• At a minimum,these results must include quarterly testing for a 12-month period within the past 1 year using multiple species(minimum of two
species),or the results from four tests performed at least annually in the four and one-half years prior to the application,provided the results
show no appreciable toxicity,and testing for acute and/or chronic toxicity,depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition,this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition,submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity,provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation,if one was conducted.
• If you have already submitted any of the information requested in Part E,you need not submit it again. Rather,provide the information
requested in question E.4 for previously submitted information. If EPA methods were not used,report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below,they may be submitted in place of Part E.
If no biomonitoring data is required,do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
complete.
E.1. Required Tests. *POLLUTANT ANALYSIS SCAN RESULTS ATTACHED
Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
❑ chronic ❑ acute
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one
column per test(where each species constitutes a test). Copy this page if more than three tests are being reported.
Test number: Test number: Test number:
a. Test information.
Test Species&test method number
Age at initiation of test
Outfall number
Dates sample collected
Date test started
Duration
b. Give toxicity test methods followed.
Manual title
Edition number and year of publication
Page number(s)
c. Give the sample collection method(s)used. For multiple grab samples,indicate the number of grab samples used.
24-Hour composite
Grab
d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection
After dechlorination
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 15 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
Test number: Test number: Test number:
e. Describe the point in the treatment process at which the sample was collected.
Sample was collected:
f. For each test,include whether the test was intended to assess chronic toxicity,acute toxicity,or both
Chronic toxicity
Acute toxicity
g. Provide the type of test performed.
Static
Static-renewal
Flow-through
h. Source of dilution water. If laboratory water,specify type;if receiving water,specify source.
Laboratory water
Receiving water
i. Type of dilution water. If salt water,specify"natural"or type of artificial sea salts or brine used.
Fresh water
Salt water
j. Give the percentage effluent used for all concentrations in the test series.
k. Parameters measured during the test. (State whether parameter meets test method specifications)
pH
Salinity
Temperature
Ammonia
Dissolved oxygen
I. Test Results.
Acute:
Percent survival in 100%
effluent
LC50
95%C.I. % % %
Control percent survival % %
Other(describe)
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 16 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS VVVVTP, NC0020800 RENEWAL HIWASSEE
Chronic:
NOEC
IC25 % % %
Control percent survival
Other(describe)
m. Quality Control/Quality Assurance.
Is reference toxicant data available?
Was reference toxicant test within
acceptable bounds?
What date was reference toxicant test j j /
run(MM/DD/YYYY)?
Other(describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ❑ No If yes,describe:
E.4. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information,or information regarding the
cause of toxicity,within the past four and one-half years,provide the dates the information was submitted to the permitting authority and a summary
of the results.
Date submitted: / / (MM/DD/YYYY)
Summary of results: (see instructions)
,, END OF PART E.
REFER TO THE APPLICATION OVERVIEW(PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 17 of 22

RIVER BASIN:PERMIT ACTION
FACILITY NAME AND PERMIT NUMBER: REQUESTED:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
SUPPLEMENTAL P.I ATION INF ORMAT Ot
PART F.JNDUSTRI R`D(SCO UG AND RCR
,idA/CERCLA WASTES
All treatment works receiving discharges from significant industrial users or which receive RCRA,CERCLA,or other remedial wastes must
complete part F.
GENERAL INFORMATION:
F.1. Pretreatment program. Does the treatment works nave,or is subject to an approved pretreatment program?
Yes •No
F.2. Number of Significant Industrial Users(SIUs)and Categorical Industrial Users(ClUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works.
a. Number of non-categorical SIUs. 0
b. Number of Gills. 0
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU. If more than one SIU discharges to the treatment works,copy questions F.3 through F.8 and
provide the information requested for each SIU.
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
Name:
Mailing Address:
F.4. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
F.5. Principal Product(s)and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
Principal product(s):
Raw material(s):
F.6. Flow Rate.
a. Process wastewater flow rate. indicate the average daily volume of process wastewater discharge into the collection system in gallons per
day(gpd)and whether the discharge is continuous or intermittent.
gpd ( continuous or intermittent) `PERMITTED FLOW
b. Non-process wastewater flow rate. Indicate the average daily volume of non-process wastewater flow discharged into the collection system
in gallons per day(gpd)and whether the discharge is continuous or intermittent.
_ gpd ( continuous or intermittent)
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following:
a. Local limits Yes No
b. Categorical pretreatment standards Yes No
If subject to categorical pretreatment standards,which category and subcategory?
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 18 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
F.8. Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems(e.g.,
upsets,interference)at the treatment works in the past three years?
❑ Yes ® No If yes,describe each episode.
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL,OR DEDIC)11/4711PHIIM
PELINE:
F.9. RCRA Waste. Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck,rail or dedicated pipe?
❑ Yes ® No(go to F.12)
F.10. Waste transport. Method by which RCRA waste is received(check all that apply):
O Truck 0 Rail 0 Dedicated Pipe
F.11. Waste Description. Give EPA hazardous waste number and amount(volume or mass,specify units).
EPA Hazardous Waste Number Amount Units
CERCLA(SUPERFUND)WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
F.12. Remediation Waste. Does the treatment works currently(or has it been notified that it will)receive waste from remedial activities?
O Yes(complete F.13 through F.15.) ® No
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates(or is excepted to origniate in
the next five years).
F.14. Pollutants. List the hazardous constituents that are received(or are expected to be received). Include data on volume and concentration,if
known. (Attach additional sheets if necessary.)
F.15. Waste Treatment.
a. Is this waste treated(or will be treated)prior to entering the treatment works?
❑ Yes ❑ No
If yes,describe the treatment(provide information about the removal efficiency):
b. Is the discharge(or will the discharge be)continuous or intermittent?
O Continuous 0 Intermittent If intermittent,describe discharge schedule.
END OF PART F.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 19 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 RENEWAL HIWASSEE
SUPPLEMENTAL APPLICATION INFORMATION
PART G. COMBINED SEWER SYSTEMS
If the treatment works has a combined sewer system,complete Part G.
G.1. System Map. Provide a map indicating the following: (may be included with Basic Application Information)
a. All CSO discharge points.
b Sensitive use areas potentially affected by CSOs(e.g.,beaches,drinking water supplies,shellfish beds,sensitive aquatic ecosystems,and
outstanding natural resource waters).
c. Waters that support threatened and endangered species potentially affected by CSOs.
G.2. System Diagram. Provide a diagram,either in the map provided in G.1 or on a separate drawing,of the combined sewer collection system that
includes the following information.
a. Location of major sewer trunk lines,both combined and separate sanitary.
b. Locations of points where separate sanitary sewers feed into the combined sewer system.
c. Locations of in-line and off-line storage structures.
d. Locations of flow-regulating devices.
e. Locations of pump stations.
CSO OUTFALLS:
Complete questions G.3 through G.6 once for each CSO discharge point.
G.3. Description of Outfall.
a. Outfall number
b. Location
(City or town,if applicable) (Zip Code)
(County) (State)
(Latitude) (Longitude)
c. Distance from shore(if applicable) ft.
d. Depth below surface(if applicable) ft.
e. Which of the following were monitored during the last year for this CSO?
❑ Rainfall 0 CSO pollutant concentrations ❑ CSO frequency
❑ CSO flow volume 0 Receiving water quality
f. How many storm events were monitored during the last year?
G.4. CSO Events.
a. Give the number of CSO events in the last year.
events (0 actual or❑approx.)
b. Give the average duration per CSO event.
hours (❑actual or❑approx.)
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 20 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
ANDREWS WWTP, NC0020800 I RENEWAL HIWASSEE
c. Give the average volume per CSO event.
million gallons(0 actual or 0 approx.)
d. Give the minimum rainfall that caused a CSO event in the last year
Inches of rainfall
G.5. Description of Receiving Waters.
a. Name of receiving water:
b. Name of watershed/river/stream system:
United State Soil Conservation Service 14-digit watershed code(if known):
c. Name of State Management/River Basin:
United States Geological Survey 8-digit hydrologic cataloging unit code(if known):
G.6. CSO Operations.
Describe any known water quality impacts on the receiving water caused by this CSO(e.g.,permanent or intermittent beach closings,permanent or
intermittent shell fish bed closings,fish kills,fish advisories,other recreational loss,or violation of any applicable State water quality standard).
END OF PART G.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
EPA Form 3510-2A(Rev.1-99). Replaces EPA forms 7550-6&7550-22. Page 21 of 22

Additional information,if provided,will appear on the following pages.
For Section
NPDES FORM 2A Additional Information

r - . 1
Annual Monitoring and Pollutant Scan
P: it No. CO 4D VC Month '1-�«rbk-,C
0111 tfall DC t Year 5+i q
Facili Name: Town of Andrews WWTP
Date ./ .ampling: 12/3/2019
Analy IF al Laboratory: Earth Environmental Services; Environmental Testing Solutions; Rogers & Callcott
Sample Analytical Quantitation Sample Units of Number of
Para !! er Type Method Level Result Measurement samples
Amm.J a (as N) Composite SM4500NH3F 0.1 2.3 mg/1 1
DissolI d oxygen Composite SM4500-0 G 0.1 8.6 mg/1 1
Nitrat: l Nitrite Composite SM4500-NO3F 0.20 2.8 mg/1 1
Total '�-ldahl nitrogen Composite 351.2 0.10 4.94 mg/1 1
Total i osphorus Composite SM4500-P-F 0.020 1.4 mg/1 1
Total .i solved solids Composite SM2540 C 20 160 mg/1 1
Hard s Composite 200.7 5.0 84 mg/1 1
Chlori; - (total residual, TRC) Grab SM 4500 CL G 10 18 ug/1 1
J
Oil an: grease Grab 1664A 5.0 ND mg/1 1
Metal- total recoverable), cyanide and total phenols
Antimi y Composite 200.8 5.0 ND ug/1 1
Arsen ; Composite 200.8 10.0 ND ug/1 1
Beryll . Composite 200.8 1.0 ND ug/1 1
Cadm Jm Composite 200.8 1.0 ND ug/1 1
Chro m Composite 200.8 5.0 ND ug/1 1
Coppe Composite 200.8 2.0 4.0 ug/1 1
Lead , Composite 200.8 5.0 ND ug/1 1
Mercu • Composite 1631 E 0.500 8.56 ng/L 1
Nickel Composite 200.8 10.0 ND ug/1 1
Seleni Composite 200.8 10.0 ND ug/1 1
Silver Composite 200.8 1.0 ND ug/1 1
Thalli I Composite 200.8 0.5 ND ug/1 1
Zinc Composite 200.8 10.0 27 ug/1 1
Cyani.11 Grab SM4500 CN-E 0.005 0.005 mg/1 1
Total .1I enolic compounds Grab 420.1 0.005 ND mg/1 1
Vola• ii organic compounds
Acrole CI Grab 624 5.0 ND ug/1 1
Acrylo trile Grab 624 5.0 ND ug/1 1
Benzes'`; Grab 624 2.0 ND ug/1 1
Brom.iirm Grab 624 2.0 ND ug/1 1
Carbo`I etrachloride Grab 624 2.0 ND ug/1 1
ch1or•NZZIIIIIIMIIIII Grab 624 2.0 ND ug/1 1
Chlor.i bromomethane Grab 624 2.0 ND ug/1 1
Chlor. �hane Grab 624 2.0 ND ug/1 1
2-chlo 1pi ethylvinyl ether Grab 624 5.0 ND ug/1 1
Chlor.Arm Grab 624 2.0 3.3 ug/1 1 j
Pichlo;l bromomethane Grab 624 2.0 ND ug/1 1 i
1,1-di.jloroethane Grab 624 2.0 ND ug/1 1
1,2-di.iloroethane Grab 624 2.0 ND ug/1 1
=I2-dichloroethylene Grab 624 2.0 ND ug/1 1
F. - DMR- PPA-1 Page 1
I

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Annual Monitoring and Pollutant Scan
Pe-mit No. Month
Ot tfall Year
4-chlorophenyl phenyl ether Grab 625 5.0 ND ug/1 1
Sample Analytical Quantitation Sample Units of Number of
Parameter Type Method Level Result Measurement samples
Base- ieutral compounds (cont.)
Chrysene Grab 625 5.0 ND ug/1 1
Di-n-M:tyl phthalate Grab 625 5.0 ND ug/1 1
Di-n-oc`yl phthalate Grab 625 5.0 ND ug/1 1
Dibenzx(a,h)anthracene Grab 625 5.0 ND ug/1 1
1,2-dithlorobenzene Grab 625 5.0 ND ug/1 1
1,3-diAllorobenzene Grab 625 5.0 ND ug/1 1
1,4-dicklorobenzene Grab 625 5.0 ND ug/1 1
3,3-dithlorobenzidine Grab 625 5.0 ND ug/1 1
Diethy: phthalate Grab 625 5.0 ND ug/1 1
Dimet}-j1 phthalate Grab 625 5.0 ND ug/1 1
2,4-dir. trotoluene Grab 625 5.0 ND ug/1 1
2,6-dir. trotoluene Grab 625 5.0 ND ug/1 1
1,2-diF`ienylhydrazine Grab 625 5.0 ND ug/1 1
Fluorarthene Grab 625 5.0 ND ug/1 1
Fluorerie Grab 625 5.0 ND ug/1 1
Hexac-.orobenzene Grab 625 5.0 ND ug/1 1
Hexac.- .orobutadiene Grab 625 5.0 ND ug/1 1
Hexac l orocyclo-pentadiene Grab 625 5.0 ND ugh 1 1
Hexac`-i.oroethane Grab 625 5.0 ND ug/1 1
Indeno;1,2,3-cd)pyrene Grab 625 5.0 ND ug/1 1
Isophcrone Grab 625 5.0 ND ug/1 1
Naphtr a.lene Grab 625 5.0 ND ug/1 1
Nitrobenzene Grab 625 5.0 ND ug/1 1
N-nitre odi-n-propylamine Grab 625 5.0 ND ug/1 1
N-nitrqiodimethylamine Grab 625 5.0 ND ug/1 1
N-nitree odiphenylamine Grab 625 5.0 ND ug/1 1
PhenaAthrene Grab 625 5.0 ND ug/1 1
Pyrene Grab 625 5.0 ND ug/1 1
1,2,4,-1.-ichlorobenzene Grab 625 5.0 ND ug/1 1
I certify under penalty of law that this document and all attachments were prepared under my direction
and si pervision in accordance with a system to design to assure that qualified perdonnel properly
gather and evaluat the information submitted. Based on my inquiry of the person or persons that
manage the system, or those persons directly responsibel for gathering the information, the
infoi ivation submitted is , to the best of my knowledge and belief, true, accurate and complete. I am
aware 'hat there are significant penalties for submitting false information, including the
possibility of fines and imprisonment for knowing violations.
Authorized Representative name
Signature
F --m - DMR- PPA-1 Page 3

c. - q�7 _ L'4% PO Box 7565
:, l " tX Asheville,NC 28802
Phone: [phone]
Fax: [phone]
y;, Environmental Testing Solutions,Inc. Certificate of Analysis
Project name: Earth Environmental Services Project number: 191203.501
Collection date: 3-Dec-19
Date received: 3-Dec-19
Sample identification: #121908 AND PPA Sample number: 194547
Parameter Method Result RL Units Date Analyst Footnotes
Analyzed
Cyanide SM 4500 CN E 0.005 0.005 mg/L 9-Dec-19 AMJ 1
Oil&Grease(HEM) 1664A <5.0 5.0 mg/L 16-Dec-19 CTS 1
VOC by 624 See Attached Sheet µg/L 5-Dec-19 JAR 1
Dissolved Solids,Total SM 2540 C 160 20 mg/L 5-Dec-19 KEK
Hardness,Total SM 2340 C 84 5.0 mg/L 7-Dec-19 BSC
Phenolics 420.1 <0.005 0.005 mg/L 12-Dec-19 ELN 1
Antimony 200.8 <0.005 0.005 mg/L 16-Dec-19 MLR 1
Arsenic 200.8 <0.010 0.010 mg/L 16-Dec-19 MLR 1
Beryllium 200.8 <0.001 0.001 mg/L 16-Dec-19 MLR 1
Cadmium 200.8 <0.001 0.001 mg/L 16-Dec-19 MLR 1
Chromium 200.8 <0.005 0 005 mg/L 16-Dec-19 MLR 1
1 Copper 200.8 0.004 0.002 mg/L 16-Dec-19 MLR 1
Lead 200.8 <0.005 0.005 mg/L 16-Dec-19 MLR 1
Nickel 200.8 <0.010 0.010 mg/L 16-Dec-19 MLR 1
Selenuim 200.8 <0.010 0.010 mg/L 16-Dec-19 MLR 1
Silver 200.8 <0.001 0.001 mg/L 9-Dec-19 MLR 1
Thallium 200.8 <0.0005 0.0005 mg/L 16-Dec-19 MLR 1
Zinc 200.8 0.027 0.010 mg/L 16-Dec-19 MLR 1
SVOC by 625 See Attached Sheet ug/L 10-Dec-19 RJM 1
Sample identification: #191209 AND-LLHG Sample number: 194548
Parameter Method Result RL Units Date Analyst Footnotes
Analyzed
Mercury 1631 E 8.56 0.500 ng/L 11-Dec-1 9 .TMF 2
Sample identification: #121908 AND Blank Sample number: 194549
Parameter Method Result RL Units Date Analyst Footnotes
Analyzed I
Mercury 1631 E <0.500 0.500 nw/L 11-Dec-19 JMF 2
This report should not be reproduced,exept in its entirety,without the written consent of Environmental Testing Solutions.Inc.
The results in this report relate only to the samples submitted for analysis.

r •
• PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc. Certificate of Analysis
Footnotes:
RL=Reporting Limit. Values are reported down to the Reporting Limit only.
1. Sample analyzed by Rogers&Callcott Environmental.
2. Sanple analzyed by Shealy Environmental Services,Inc.
Date reviewed: I A'4V,t ) NC Certification Number: 600
Data reviewed by: Kelley E.Keenan SC Certification Number: 99053
NC Drinking Water Certification Number: 37786
Signature:
This report should not be reproduced,exept in its entirety,without the written consent of Environmental Testing Solutions,Inc.
The results in this report relate only to the samples submitted for analysis.

Annual Monitoring and Pollutant Scan
Permit No. NCm-159OO Month Apr1
Outfall CIN Year aOaa
Facility Name: Town of Andrews WWTP
Date of sampling: 4/1/2020
Analytical Laboratory: Earth Environmental Services; Environmental Testing Solutions; Rogers & Callcott
• ,, s i - t t I17 Y - , 7�4 3
a�"< .".,, th.o . �ui A K3 8 s a i
Ammonia (as N) Composite SM4500NH3F 0.1 1.1 mg/1 1
Dissolved oxygen Composite SM4500-0 G 0.1 8.7 mg/1 1
Nitrate/Nitrite Composite SM4500-NO3F 0.20 2.69 mg/1 1
Total Kjeldahl nitrogen Composite 351.2 0.10 2.25 mg/1 1
Total Phosphorus Composite SM4500-P-F 0.020 0.8 mg/1 1
Total dissolved solids Composite SM2540 C 20 140 mg/1 1
Hardness Composite 200.7 5.0 73 mg/1 1
Chlorine (total residual, TRC) Grab SM 4500 CL G 10 17 ug/1 1
Oil and grease Grab 1664A 5.0 ND mg/1 1
Meths(to° _ ,w: a 1' .d total C o 'ii Z r Pt iZ ,>„ r ,' ,,.., ' .tt <...u. 4 f
Antimony Composite 200.8 5.0 ND ug/1 1
Arsenic Composite 200.8 10.0 ND ug/1 1
Beryllium Composite 200.8 1.0 ND ug/1 1
Cadmium Composite 200.8 1.0 ND ug/1 1
Chromium Composite 200.8 5.0 ND ug/1 1
Copper Composite 200.8 2.0 ND ug/1 1
Lead Composite 200.8 5.0 ND ug/1 1
Mercury Composite 1631 E 0.500 5.30 ng/L 1
Nickel Composite 200.8 10.0 ND ug/1 1
Selenium Composite 200.8 10.0 10 ug/1 1
Silver Composite 200.8 1.0 ND ug/1 1
Thallium Composite 200.8 0.5 ND ug/1 1
Zinc Composite 200.8 10.0 18 ug/1 1
Cyanide Grab SM4500 CN-E 0.005 ND mg/1 1
Total phenolic compounds Grab 420.1 0.005 ND mg/1 1
ars
Acrolein Grab 624 5.0 ND ug/1 1
Acrylonitrile Grab 624 5.0 ND ug/1 1
Benzene Grab 624 2.0 ND ug/1 1
Bromoform Grab 624 2.0 ND ug/1 1
Carbon tetrachloride Grab 624 2.0 ND ug/1 1
Chlorobenzene Grab 624 2.0 ND ug/1 1
Chlorodibromomethane Grab 624 2.0 ND ug/1 1
Chloroethane Grab 624 2.0 ND ug/1 1
2-chloroethylvinyl ether Grab 624 5.0 ND ug/1 1
Chloroform Grab 624 2.0 ND ug/1 1
Dichlorobromomethane Grab 624 2.0 ND ug/1
1,1-dichloroethane Grab 624 2.0 ND ug/1
1,2-dichloroethane Grab 624 2.0 ND ug/1 1111
Trans-1,2-dichloroethylene Grab 624 2.0 ND ug/1 1
Form - DMR- PPA-1 Page 1

4
Annual Monitoring and Pollutant Scan
Permit No. Month
berE
Outfall Year
y. � p
' ',, i s r 4, � ':>s
Y olat o ..: , a s%s{5 x '�a`,+1,�'aat',,„Nr,'E4.,a '. ,, 3 x '. is;$ u, ea '.�c4 3td` a :: x _ ems
a -.:. sir, , 3,,„ .. .', ,aa3 n5� »•, N ssr, �� .� .�;
1,1-dichloroethylene Grab 624 2.0 ND ugh 1 1
1,2-dichloropropane Grab 624 2.0 ND ug/1 1
1,3-dichloropropylene Grab 624 1.0 ND ug/1 1
Ethylbenzene Grab 624 2.0 ND ug/1 1
Methyl bromide Grab 624 2.0 ND ug/1 1
Methyl chloride Grab 624 2.0 ND ug/1 1
Methylene chloride Grab 624 2.0 ND ug/1 1
1,1,2,2-tetrachloroethane Grab 624 2.0 ND ug/1 1
Tetrachloroethylene Grab 624 2.0 ND ug/1 1
Toluene Grab 624 2.0 ND ug/1 1
1,1,1-trichloroethane Grab 624 2.0 ND ug/1 1
1,1,2-trichloroethane Grab 624 2.0 ND ug/1 1
Trichloroethylene Grab 624 2.0 ND ug/1 1
Vinyl chloride Grab 624 2.0 ND ug/1 1
Acid» �- -, -1. t ; , 4. � �••
P-chloro-m-creso Grab 625 5.0 ND ug/l 1
2 chlorophenol Grab 625 5.0 ND ug/1 1
2,4-dichlorophenol Grab 625 5.0 ND ug/1 1
2,4-dimethylphenol Grab 625 5.0 ND ug/1 1
4,6 dinitro o cresol Grab 625 5.0 ND ug/l 1
2,4-dinitrophenol Grab 625 10.0 ND ug/1 1
2-nitrophenol Grab 625 5.0 ND ug/1 1
4-nitrophenol Grab 625 5.0 ND ug/1 1
Pentachlorophenol Grab 625 5.0 ND ug/1 1
Phenol Grab 625 5.0 ND ug/1 1
2,4,6 trichlorophenol Grab 625 5.0 ND ug/1 1
:, , s , u' � i'1 i;., ,.,.. .. ? - °a'' I. ,. •�.'ti we.6x. , w^hs, a' '`�a d C.:
Acenaphthene Grab 625 5.0 ND ug/1 1
SkiiAcenaphthylene Grab 625 5.0 ND ug/1 1
Anthracene Grab 625 5.0 ND ug/I 1
Benzidine Grab 625 80 ND ug/1 1
Benzo(a)anthracene Grab 625 5.0 ND ug/1 1
Benzo(a)pyrene Grab 625 5.0 ND ug/1 1
3,4 benzofluoranthene Grab 625 5.0 ND ug/1 1
Benzo(ghi)perylene Grab 625 5.0 ND ug/1 1
Benzo(k)fluoranthene Grab 625 5.0 ND ug/1 1
ug/1 1
Bis (2 chloroethoxy) methane Grab 625 5.0 ND
Bis (2-chloroethyl) ether Grab 625 5.0 ND ug/1 1
Bis (2-chloroisopropyl) ether Grab 625 5.0 ND ug/1 1
1
Bis (2-ethylhexyl) phthalate Grab 625 10.0 ND ug/1
1
4-bromophenyl phenyl ether Grab 625 5.0 ND ug/1
Butyl benzyl phthalate Grab 625 5.0 ND ug/1 1
2-chloronaphthalene Grab 625 5.0 ND ug/1 1
4-chlorophenyl phenyl ether Grab 625 5.0 ND ug/1 1
Form - DMR- PPA-1 Page 2

Annual Monitoring and Pollutant Scan
Permit No. Month
Outfall Year
l *3�D J.r ,✓°�Y it ..., ,f,A�'/e41 , sae Y�'. zA1
Chrysene Grab 625 5.0 ND ug/l 1
Di-n-butyl phthalate Grab 625 5.0 ND ug/1 1
Di-n-octyl phthalate Grab ▪ 625 5.0 ND ug/1 1
Dibenzo(a,h)anthracene Grab 625 5.0 ND ug/1 1
1,2-dichlorobenzene Grab • 625 2.0 ND ug/1 1
1,3-dichlorobenzene Grab 625 2.0 ND ug/1 1
1,4-dichlorobenzene Grab 625 2.0 ND ug/1 1
3,3-dichlorobenzidine Grab 625 5.0 ND ug/1 1
Diethyl phthalate Grab 625 5.0 ND ug/1 1
Dimethyl phthalate Grab 625 5.0 ND ug/1 1
2,4-dinitrotoluene Grab 625 5.0 ND ug/1 l
2,6-dinitrotoluene Grab 625 5.0 ND ug/1 1
1,2-diphenylhydrazine Grab 625 5.0 ND ug/1 1
Fluoranthene Grab 625 5.0 ND ug/1 1
Fluorene Grab 625 5.0 ND ug/1 1
Hexachlorobenzene Grab 625 5.0 ND ug/1 1
Hexachlorobutadiene Grab • 625 5.0 ND ug/1 1
-
Hexachlorocyclo-pentadiene Grab 625 10.0 ND ug/1 1
Hexachloroethane Grab 625 5.0 ND ug/1 1
Indeno(1,2,3-cd)pyrene Grab 625 5.0 ND ug/1 1
Isophorone Grab 625 5.0 ND ug/1 1
Naphthalene Grab • 625 5.0 ND ug/1 1
Nitrobenzene Grab 625 5.0 ND ug/1 1
N-nitrosodi-n-propylamine Grab 625 5.0 ND ug/1 1
N-nitrosodimethylamine Grab - 625 5.0 ND ug/1 1
N-nitrosodiphenylamine Grab 625 5.0 ND ug/1 1
Phenanthrene Grab 625 5.0 ND ug/1 1
Pyrene Grab 625 5.0 ND ug/1 1
1,2,4,-trichlorobenzene Grab 625 2.0 ND ug/1 1
I certify under penalty of law that this document and all attachments were prepared under my direction
and supervision in accordance with a system to design to assure that qualified perdonnel properly
gather and evaluat the information submitted. Based on my inquiry of the person or persons that
manage the system, or those persons directly responsibel for gathering the information, the
information submitted is , to the best of my knowledge and belief, true, accurate and complete. I am
aware that there are significant penalties for submitting false information, including the
possibility of fines and imprisonment for knowing violations.
Authorized Representative name
Signature
Date
Form - DMR- PPA-1 Page 3

r
P it No. Dc>1D%C�
Annual Monitoring and Pollutant Scan
Month Se r4; niter
O tfall cc t Year ava 1
Facili Name: Town of Andrews WWTP
Date sampling: 9/7/2021
Analy ' al Laboratory: Earth Environmental Services; Environmental Testing Solutions; Rogers & Callcott
9 Sample Analytical Quantitation Sample Units of Number of
Para t,ter Type Method Level Result Measurement samples
Ammo's'a (as N) Composite SM4500NH3F 0.1 3.2 mg/1 1
Dissol •d oxygen Composite SM4500-0 G 0.1 6.8 mg/1 1
Nitrat•i Nitrite Composite SM4500-NO3F 0.10 1.05 mg/1 1
Total *ldahl nitrogen Composite 351.2 0.10 8.00 mg/I 1
Total "hosphorus Composite SM4500-P-F 0.050 1.6 mg/1 1
Total •.ssolved solids Composite SM2540 C 20 140 mg/1 1
Hard , ss Composite 200.7 5.0 82 mg/1 1 1
Chlori g (total residual, TRC) Grab SM 4500 CL G 10 ND ug/1 1 _ 1
Oil anc. grease Grab 1664A 5.0 ND mg/1 1
Metal(total,recoverable),cyanide and total phenols
Antimony Composite 200.8 10.0 ND I ug/1 1
lArsenic Composite 200.8 10.0 ND ug/1 1
Beryllifum Composite 200.8 1.0 ND ug/1 1
Cadm1 -m Composite 200.8 1.0 ND ug/1 1
Chrom am Composite 200.8 5.0 ND ug/1 i 1
Coppe Composite 200.8 2.0 8.0 ug/1 1
Lead ' Composite 200.8 2.0 ND ug/1 1
Mercui Composite 1631 E 2.500 9.01 ng/L 1
Nickel Composite 200.8 10.0 ND ug/1 1
Seleni -n Composite 200.8 10.0 10 ug/1 1
Silver Composite 200.8 1.0 ND ug/1 1
Thalli 1 Composite 200.8 0.5 ND ug/1 1 ___I
Zinc Composite 200.8 10.0 42 ug/1 1
Cyanid; Grab SM4500 cN-E 0.005 ND ( mg/1 1
Total phenolic compounds Grab 420.1 0.005 ND mg/1 1
Volatille organic compounds
Acrole - Grab 624 5.0 ND ug/1 1
Acrylo.-..trile Grab 624 5.0 ND ug/1 1
Benzene Grab 624 2.0 ND ug/1 1
---I
Bromoform Grab 624 2.0 ND ug/1 1
Carbon tetrachloride Grab 624 2.0 ND ug/l 1
Chlorobenzene Grab 624 2.0 ND ug/1 1
Chlorodibromomethane Grab 624 2.0 ND ug/1 1
Chloroehane Grab r 624 2.0 ND ug/1 1
2-chloroethylvinyl ether Grab 624 5.0 ND ug/1 1
Chlorof nn Grab 624 2.0 ND ug/1 1
Dichlor bromomethane Grab 624 2.0 ND ug/1 1
1,1-dic:.loroethane Grab 624 2.0 ND ug/1 1
1,2-dic`.'.1oroethane Grab 624 2.0 ND ug/1 1
Trans-:,2-dichloroethylene Grab 624 2.0 ND ug/1 1.
Form - DMR- PPA-1 Page 1

7
Annual Monitoring and Pollutant Scan
P it No. Month
01 fall Year
Sample Analytical Quantitation Sample Units of Number of
Pa er Type Method Level Result Measurement samples
Volat' organic compounds(Cont.)
1,1-di ;loroethylene Grab 624 2.0 ND ug/1 1
1,2-dic tloropropane Grab 624 2.0 ND ug/1 1
1,3-dictloropropylene Grab 624 1.0 ND ug/1 1
Ethylbehzene Grab 624 2.0 ND ug/1 1
Methy: bromide Grab 624 2.0 ND ugh 1 1
Methy.chloride Grab 624 2.0 ND ug/1 1
Methy:rne chloride Grab 624 2.0 ND ug/1 1
1,1,2,2 etrachloroethane Grab 624 2.0 ND ug/1 1
Tetrac .oroethylene Grab 624 2.0 ND ug/1 1
Toluer- Grab 624 2.0 ND ug/1 1
1,1,1- chloroethane Grab 624 2.0 ND ug/1 1
1,1,2_tf_chloroethane Grab 624 2.0 ND ug/1 1
Trichlohoethylene Grab 624 2.0 ND ug/1 1
Vinyl chloride Grab 624 I 2.0 ND ug/1 1
Acid actable compounds
P-chlo -m-creso Grab 625 5.0 ND ug/1 1
2-chlo phenol Grab 625 5.0 ND ug/1 1
2,4-di lorophenol Grab 625 5.0 ND ug/1 1
2,4-di thylphenol Grab 625 5.0 ND ug/1 1
4,6-di ' ro-o-cresol Grab 625 5.0 ND ug/1 1
2,4-di iorophenol Grab 625 10.0 ND ug/1 1
2-nitro henol Grab 625 5.0 ND ug/1 1
4-nitro henol Grab 625 5.0 ND ug/1 1
Pentac .lorophenol Grab 625 5.0 ND ug/1 1
Phenol Grab 625 5.0 ND ug/1 1
2,4,6-tr.chlorophenol Grab 625 5.0 ND ug/1 1
Base-n E utral compounds
Acenap.thene Grab 625 5.0 ND ug/1 1
Acenaphthylene Grab 625 5.0 ND ug/1 1
Anthraoene Grab 625 5.0 ND ug/1 1
Benzidine Grab 625 80 ND ug/1 1
Benzo(aianthracene Grab 625 5.0 ND ug/1 1
Benzo(4pyrene Grab 625 5.0 ND ug/1 1
3,4 berizofluoranthene Grab 625 5.0 ND ug/1 1
Benzo( ai)perylene Grab 625 5.0 ND ug/1 1
Benzo(llfluoranthene Grab 625 5.0 ND ug/1 1
Bis (2- .aloroethoxy) methane Grab 625 5.0 ND ug/1 1
Bis (2- .iloroethyl) ether Grab 625 5.0 ND ug/1 1
Bis (2- `aloroisopropyl) ether Grab 625 5.0 ND ug/1 1
Bis (2-ahylhexyl) phthalate Grab 625 10.0 12.1 ug/1 1
4-bro it i phenyl phenyl ether Grab 625 5.0 ND ugh 1 1
Butyl +° nzyl phthalate Grab 625 5.0 ND ug/1 1.
2-chlo 'naphthalene Grab 625 5.0 ND ug/1 1
4-chlo,+phenyl phenyl ether Grab 625 5.0 ND ugh]. 1
Firm - DMR- PPA-1 Page 2

Annual Monitoring and Pollutant Scan
Permit No. Month
Oatfall Year
Sample Analytical Quantitation Sample Units of Number of
Paramleter Type Method Level Result Measurement samples
Ease-neutral compounds(cont.)
Chrysc eJ Grab 625 5.0 ND ug/1 1
Di-n-b-4 tyl phthalate Grab 625 5.0 ND ugh 1 1
Di-n-ociyl phthalate Grab 625 5.0 ND ug/1 1
Dibenzo(a,h)anthracene Grab 625 5.0 ND ug/1 1
1,2-dicl:lorobenzene Grab 624 2.0 ND ug/1 1
1,3-dicl:lorobenzene Grab 624 2.0 ND ug/1 1
1,4-dicT.lorobenzene Grab 624 2.0 ND ug/1 1
3,3-dicr.lorobenzidine Grab 625 5.0 ND ug/1 1
Diethy'. 3hthalate Grab 625 5.0 ND ug/1 1
Dimethyl phthalate Grab 625 5.0 ND ug/1 1
2,4-dini:rotoluene Grab 625 5.0 ND ug/1 1
2,6-dinisotoluene Grab 625 5.0 ND ug/1 1
1,2-dipr.enylhydrazine Grab 625 5.0 ND ug/1 1
Fluoran hene Grab 625 5.0 ND ugh 1 1
Fluorene Grab 625 5.0 ND ug/1 1
Hexachlarobenzene Grab 625 5.0 ND ug/1 1
Hexachbrobutadiene Grab 625 5.0 ND ug/1 1
Hexacl±rocyclo-pentadiene Grab 625 5.0 ND ug/1 1
Hexachbroethane Grab 625 5.0 ND ug/1 1
Indeno(: ,2,3-cd)pyrene Grab 625 5.0 ND ug/1 1
Isophorone Grab 625 5.0 ND ug/1 1
Naphthzlene Grab 625 5.0 ND ug/1 1
Nitrobe-'.zene Grab 625 5.0 ND ug/1 1
N-nitrospdi-n-propylamine Grab 625 5.0 ND ug/1 1
N-nitrosodimethylamine Grab 625 5.0 ND ug/1 1
N-nitrosDdiphenylamine Grab 625 5.0 ND ug/1 1
Phenanthrene Grab 625 5.0 ND ug/1 1
Pyrene Grab 625 5.0 ND ug/1 1
1,2,4,- i'ichlorobenzene Grab 624 2.0 ND ug/1 1
I certi f' under penalty of law that this document and all attachments were prepared under my direction
and supervision in accordance with a system to design to assure that qualified perdonnel properly
gather and evaluat the information submitted. Based on my inquiry of the person or persons that
manage the system, or those persons directly responsibel for gathering the information, the
information submitted is , to the best of my knowledge and belief, true, accurate and complete. I am
aware tziat there are significant penalties for submitting false information, including the
possiblifity of fines and imprisonment for knowing violations.
Authorized Representative name
Signature
Date
F .'in - DMR- PPA-1 Page 3

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•
PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
Environmental Toning Solutions,Inc,
Certificate of Analysis
RL=Reporting Limit. Values are reported down to the Reporting Limit only.
1. Sample analzyed by Rogers&Callcott Environmental,Inc.
2. Sample analzyed by Gel Laboratories,LLC.
Date reviewed: /0 0 9 2/ NC Certification Number: 600
Data reviewed by: Kelley E.Keenan
SC Certification Number: 99053
NC Drinking Water Certification Number: 37786
Signature:
This report should not be reproduced,exept in its entirety,without the written consent of Environmental Testing Solutions,Inc.
The results in this report relate only to the samples submitted for analysis.

i `-k> PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
September 27, 2019
Ms. Wanda Ladd
Earth Environmental Services
75 Bison Lane
Murphy, NC 28906
RE: ETS PROJECT NUMBER 14460
Dear Ms.Wanda Ladd:
Enclosed are toxicity test results for samples from the Andrews WWTP received by
Environmental Testing Solutions, Inc. September 03 through September 05, 2019.
Parameter Test Procedure EPA Method
Final
Code Number. Result
North Carolina Ceriodaphnia Chronic Effluent
TGP3B Toxicity Procedure EPA-821-R-02-013 PASS
(Ceriodaphnia Pass/Fail Toxicity Test)
If this test was performed as an NPDES requirement or by Administrative Letter, please enter a
P on the Effluent Discharge Monitoring Form (MR-1) for the collection date
September 03, 2019 using the parameter code TGP3B.
Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT-3) by
October 31, 2019.
If you have any questions concerning these results, please feel free to contact me.
Sincerely,
Ji Sumner
Laboratory Director
This report should not be reproduced,except in its entirety,without the written consent of Environmental Testing,Solutions, Inc.
The results in this report relate only to the samples submit.ed for analysis.
North Carolina Certificate Numbers: Biological Analyses: 37,Drinking Water: 37786, Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001

* PO Box 7565
Asheville,NC 28802
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: September 27,2019
Facility: Earth Environmental Services NPDES#: NC-0020800 Pipe#: 001 County: Cherokee
Andrews WWTP
Laboratory Performing Test: Environmental Testing nc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: Project 14460
Samples: 190903.06,190905,06
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 09-04-19 09-11-19 1115 0728
Sample Information Sample 1 Sample 2 Control
Collection start date: 09-02-19 09-04-19 Test Information start Rcnc..al I Renewal 2 Sian Rencnal I Rencnal 2
Grab: Treatment: 13% 13% 13% Control Control Control
Composite duration: 24.75-h 24.75-h Initial pH(SU): 7,81 7.87 7.89 7.86 7.87 7.78
Alkalinity(mg/L CaCO3): 63 Final pH(SU): 7.99 7.97 7.94 7.98 7.99 7.91
Hardness(mg/L CaCO3): 88 Initial DO(mg/L): 7.8 8.0 7.9 8.0 7.8 7.8
Conductivity(µmhos/cm): 283 304 308.3 i I.304 Final DO(mg/L): 8.1 8.0 7.9 8.1 7.8 7.8
Total residual chlorine(mg/L): <0.10 <0.10 Initial Temp.(°C): 25.2 24.8 24.9 24.7 25.0 24.8
Sample Temp.at Receipt(°C): 0.4 0.6 Final Temp.(°C): 25.0 25.2 24.9 25.2 24,8 25.1
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
cumber of Young Produced 31 31 32 29 34 34 32 31 30 29 35 29 31.4
Final Control Mortality(%): 0,0
Adult Survival: (L)ive.(D)ead L L L L L L L L L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 6.6
E ffluent Percentage 13% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Control: 0 of 12
•umber of Young Produced 32 33 32 31 31 30 33 35 29 30 30 28 31.2 lWC: o of 12
A dult Survival: (L)ive,(D)ead L L L L L L L L L L L L 0.8 Significant?: No
Reduction Final Mortality Significant at No concentration
ffluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Maan Reproduction Analyses
h umber of Young Produced Reproduction LOEC >13%
II
dolt Survival: (L)ive,(D)ead Reproduction NOEC: 1 3%
%Reduction
Overall Method: Homoscedastic t
LY"fluent Percentage g Normal Distribution: Yes
-Yeatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Method. Shapiro-Wilk's
Number of Young Produced
Statistic:t 0.958
Adult Survival: (L)ive,(D)ead Critical value: 0.884
%Reduction
Equal Variances: Yes
fluent Percentage Method: F-Test
_ eatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mena Statistic: 1.126
mber of Young Produced Critical Value: 5.320
ult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
%Reduction
Method:
1 [fluent Percentage Effluent% Rank Sum Critical Sum
-eatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 I I 12 mtean 13%
imber of Young Produced
ult Survival: (L)ive.(D)ead
%Reduction
•verall Analysis:
Result: PASS LOEC: >13% NOEC: 13% ChV: >13%
fWQform AT-3(8/91)Rev. 11/95

f
t>
I ar
Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0,NC Modification-December 2010,Version 3.0)-Control Bench Sheet
IControl 6: \ Date: 09-04-19
Test Grouping Information: Test Organism Information:
Facility I Project# Organism Source: In-house Culture
b t-��11�/.41� t���� Age: <24-hours old
5 CA-t FFS tat btj taa) Source(culture board): 0Q• :\-AA b&'11 t
4 . oat lyy 11 Replicate# 1 2 3 4 5 6 7 8 9 10 11 12
r3 Astke..►,�e-As>� ,y-kh-,� Culture board cup#1S jp 3\ 33 A 31 e
2 fjN,to..2 JS t4AV0 Date and time organisms were 04•0.1t.t\ o1e4S T018%-10
1-Control born between:
Average transfer volume: <0.25 mL -
Transfer bowl information: pH(S.U.): 1-e\1
Temperature(°C): 1A•sR
Daily Renewal Information:
' Test Initiation,renewal,feeding or
Selenastrum YWT Location Randomizing
Day Date termination MHSW Batch
Time Analyst Batch Batch Incubator/Shelf Template
0 09-04-19 Initiatio/Feeding
11�5 L1 04'3,0, OV•V0-e\ pg•30-ti _ ZC,` p_e__
1 09-05-19 _Feelt.AC V,
2 09-06-19 Renewal 1/Feeding
1 e1e It 0k.3o-tl
' 3 09-07-19 Feeding
FcfInt) K
4 09-08-19 eeding
QItD
5 09-09-19 Reneaal2/Feeeding
6 09-10-19 Feeding
7 09-11-19 rerminariezn
azc
' Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst Hf j` t-t_f t. 1- -r KS
' pH(S.U.) 6(o -496 t•vT y ctei t• e 1.11
Dissolved oxygen(mg/L) e•<, et q.46 7.8 /e 7.6
Conductivity(µmhos/cm) 3oeo )l 309
Control,MHSW
`Alkalinity(mg/L CaCO3) 103 O11Sl' 09ts1`I
I 'Hardness(mg/LCaCO3) 3e
"Temperature(°C) -D1•1 *IS-1 Is• 0 L'&.& L'l.C1 -is•1
'Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation,s renewal or termination
1 by the analyst identified in the Daily Renewal Information table. Alkalinity and hardness performed by the analysts indentified on the test bench sheets and transcribed to this bench
sheet
Survival and Reproduction Data:
Observations Replicate number
Day
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality L- L_ L- L \--- L.
Renewal One IL=Live,D=dead)
Number of broods present Il- le ti 1 1- I •z. -1. 11. I1-. 1- I 1 IZ
' S Number of young produced IS 15 IL 14 t1 11 (j IS 1G 13 16 /'-I
Renewal Two Adult mortality
jL=Live,D=dead) L.. L. ` `- L L L_
0 Number of broods present c\ \ \ \ 1 1 1 t I t 1� Lt t I ,
Number of young produced b �� 15 11-115 1• 1 � 1� 1 1 S—
Final7 Total young produced 1\ 2` SL Z� 3-1
,7 `� 3A 3l j\ 3o z5 35 IA
11 Final adult mortality `
(L=Live,D=dead) V- ` I V_
X for 3rd Broods x X- >4.- x ( > 7 ?C- )(----------
Control Acceptance Criteria:
of Male Adults(<20%) 0 Mean Offspring/Female
Adults having 3rd Broods(>80%) (On• (>15 offspring/surviving female) 3(.y
%Mortality(<20%) 0)s %CV(<40%)
�l 0
SOP AT12-Revision 4-Exhibit AT12.3

1
Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0,NC Modification—December 2010,Version 3.0)-Test Bench Sheet
111
Paired with Control# I Date: -i)4 '
IIClient Earth Environmental Services NPDES# NC0020800
Facility Andrews WWTP Outfall 001
11 Project# IAAbn County Cherokee
Test Concentration (Chronic Limit) 13% Dilution mL mL Total volume
Ix preparation: Sample Dilution water mL
Samples were not aerated or treated unless otherwise noted on this form. Control,dilution water
and test renewal information are included on the Control Bench Sheet indicated above. 39 261 300
# Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst "'IC y�, �S -y s u T y�_g
pH(S.U.) 6I 't•qg t•9 �Q� Y 7,-1111 Test Dissolved oxygen(mg/L) g( 8O d',1 t•
7.?
Concentration Conductivity(µmhos/cm) Z-? Z•cr? 31z.
'Temperature(°C) IS.1-- As. p IA•cfs' 15 • 1 IA.q Z4.6k
Dissolved oxygen(mg/L) ' e3 8•a
I100% Conductivity(µmhos/cm) - 301
'Total residual chlorine(mg/L) GD• klp <p•10
Sample number Sample 1 q Op •04. Sample 2 AOq^e•0
IN 'Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation,renewal or
termination by the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the
Total Residual Chlorine Bench Sheet and transcribed to this bench sheet.
I Survival and Reproduction Data (performed at test concentration):
Day Observations Replicate number
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality
INRenewal One (t=Lwe,D=dead) L___ �- L- L L
Number of broods present 1- t L Z, 1 1 L 1 '1 -L Z 7-
5 Number of young produced 11 tS 1S 16p 1Z• pa IS is I -. t j 'C 1 t
Renewal Two Adult mortality ` ` O
(L=Live,D=dead) L- ` ` �' L. L. L.- V_ k— l_
Number of broods present 1 1 % l \ I% CCl
^ 6 t t % I 1
Number of young produced I (1 IS li �� 1-1IS rt. no
7 Total young produced 1 1 � �• :
Final 31- 3� 'IA.
J 3o •37 3S 1 5 t4 JO 1.q.
Final adult mortality L ` L. L L- k..-
(L=Live,D=dead)
Test was initiated using Sample 1. Sample 2 was used for Renewals One(day 2)and Two(day 5). Samples were diluted to the test concentration prior to use with moderately hard
synthetic water and warmed to 25.0 it 1.0°C in a warm water bath.
IComments:
ITest Results and Statistical Analyses:
Test results Statistics
%Mortality /`�'1 t-Stat or
U i• Rank Sum 0•.2I345
Mean offspring 1-Tailed 9
per female 31 •Z 2 Critical -500
%Reduction
Ifrom control O. PASS or FAIL PASS
SOP AT12-Revision 4-Exhibit AT12.2

' Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test
3 ' �x ; EPA-821-R-02-013, Method 1000.0-North Carolina Modification
Quality Control
Verification of Data Entry,Calculations,and Statistical Analyses
I_
Client: Andrews WWTP ,
Test dates: September 04- 11,2019 r? Environmental Testing Solutions,Inc.
Project number: 14460
Reveiwed by: ,
Concentration Day Survival Average Coefficient of Percent reduction
(°AO) Number of young produced by replicate number
(%) reproduction variation from control CYO
1 2 3 4 5 6 7 8 9 10 11 12 (offspring/female) (%)
Control 5 13 15 16 14 17 19 15 15 16 13 16 14
7 18 16 16 15 17 15 17 16 14 16 19 15 100 31.4 6.6 Not applicable
Total 31 31 32 29 34 34 32 31 30 29 35 29
13°Ao 5 17 15 15 16 12 14 15 17 12 15 18 12
7 15 18 17 15 19 16 18 18 17 15 12 16 100 31.2 6.2 0.8
Total 32 33 32 31 31 30 33 35 29 30 30 28
Dunnett's MSD value: 2.055 MSD= Minimum Significant Difference
PMSD: 6.5 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10th percentile) = 13%.
Upper PMSD bound determined by USEPA(90a'percentile)=47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile,respectively,of PMSD data from EPA's WET
Interlaboratory Variability Study(USEPA,2001a;USEPA,2001b).
USEPA. 2001 a,2001 b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes I and 2-Appendix.EPA-821-8-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency,Cincinnati,OH.
Andrews 09-04-19

•
•
Environmental Testing Solutions,Inc.
Ceriodaphnia Survival and Reproduction Test-Reproduction
S art Date: 9/4/2019 Test ID: CdPFCRNC Sample ID: Andrews WWTP
E-d Date: 9/11/2019 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
C:mments:
Conc-% 1 2 3 4 5 6 7 8 9 10
D-Control 31.000 31.000 32.000 29.000 34.000 34.000 32.000 31.000 30.000 29.000
D-Control 35.000 29.000
13 32.000 33.000 32.000 31.000 31.000 30.000 33.000 35.000 29.000 30.000
13 30.000 28.000
Transform:Untransformed 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
D-Control 31.417 1.0000 31.417 29.000 35.000 6.574 12
13 31.167 0.9920 31.167 28.000 35.000 6.245 12 0.305 2.508 2.055
Au Kiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution (p>0.01) 0.95846 0.884 0.36771 -0.6629
F-'Test indicates equal variances(p=0.85) 1.126 5.31963
Hypothesis Test(1-tail,0.01) MSDu MSDp MSB MSE F-Prob df
H:moscedastic t Test indicates no significant differences 2.05481 0.06541 0.375 4.02652 0.7631 1, 22
Tresatments vs D-Control
Andrews 09-04-19

_ Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0, NC Modification-December 2010,Version 3.0)-Test Bench Sheet
Paired with Control# 1 Date: 11- 041'1-0
Client Earth Environmental Services NPDES# NC0020800
Facility Andrews WWTP Outfall 001
Project# \SSS-0% County Cherokee
Test Concentration(Chronic Limit) 13% Dilution mL ml Total volume
preparation: Sample Dilution water mL
Samples were not aerated or treated unless otherwise noted on this form. Control,dilution water
and test renewal information are included on the Control Bench Sheet indicated above. 39 261 300
Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst -[ S J 7/] 7-5 ,-S 1-5 Kis
pH(S.U.) 7 go2 7.90 7,74 7 8'9 7 C85 7-2Q
Test Dissolved oxygen(mg/0 7 ••7 eo q - 7.5 7 7 1_7
Concentration Conductivity(µmhos/cm) 3/6 310 307
'Temperature(°C) 'as.\ _A__ -Li-v ZS • \ ZS •1 ZS•3
pH(S.U.) 7.'-4`-' 7.3?
Dissolved oxygen(mg/L) - 1.4 4
100% Conductivity(µmhos/cm) a50 PZ.L•(?
°Total residual chlorine(mg/L) 40,to 4 0.10
Sample number Sample Zbt1ag,0k. Sample 2 1O l LI0.tit
'Analyst identified for each day.performed pH.dissolved oxygen and conductivity measurements only. Temperatures perfonned at the time of test initiation,renewal or termination by
the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine perfonned by the analyst identified on the Total Residual
Chlorine Bench Sheet and transcribed to this bench sheet.
Survival and Reproduction Data (performed at test concentration):
Observations Replicate number Day 1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality
Renewal One (L=Live,D=dead) l - e--" l'-' 1._. L__. `--" ��
Number of broods present Li Lz Lz• t L I L L Li '� O.- b-
5 Number of young produced Ile, t +� ,� 11 IS �L� iZ `b t� l� IL,
Renewal Two Adult mortality `
(L=Live,D=dead) L L � 1_ �- ` L L L_ L L._
Number of broods present I l L1_, L_ Lt L . I� qe a ! + 15 t S t�
L< L . LL L, LLL
Number of young produced i i '� I`� tL. 1`�1
'
7 Total young produced
Final 3S N. "Cle 3 - . \ 7,4 .�\ 3\ "SA 1..°1 .I' S
Final adult mortality t
(L=Lrve,D=dead) `-' L `_ L L ~
Test was initiated using Sample 1. Sample 2 was used for Renewals One(day 2)and Two(day 5). Samples were diluted to the test concentration prior to use with moderately hard
synthetic water and warmed to 25.0±1.0°C in a warm water bath.
Comments:
Test Results and Statistical Analyses:
Test results Statistics
%Mortality t-Stat or
_ D1• _ Rank Sum -1,.SH
Mean offspring 1-Tailed
per female 31
Critical Z• Z?'
%Reduction --1A.1
PASS or FAIL ASS
from control
SOP AT12-Revision 4-Exhibit AT12.2

Environmental Testing Solutions,Inc.
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia
Entered by:
Kelley Keen
Facility: Andrews WWTP Reviewed byr
Test start date: December 09,2020 Jim ne,
Project number: 15589 ak
Sample numbers: 201208.08,201210.19
Number of young produced by replicate number Survival Average Coefficient of Percent reduction from
Concentration Day (%) reproduction variation (%) control (%)
/o
(a j 1 2 3 4 5 6 7 8 9 10 11 12 (offspring/female)
5 12 13 12 15 11 14 14 14 16 13 13 15
Control 7 14 12 16 12 16 11 13 12 12 12 15 12 100 26.6 4.4 Not applicable
Total 26 25 28 27 27 25 27 26 28 25 28 27
5 16 18 14 16 17 15 14 12 16 14 19 16
13% 7 17 13 13 16 14 19 17 19 18 15 15 19 100 31.8 7.3 -19.7
Total 33 31 27 32 31 34 31 31 34 29 34 35
Dunnett's MSD value: 1.885 MSD= Minimum Significant Difference
PMSD: 7.1 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10th percentile) =13%.
Upper PMSD bound determined by USEPA(90th percentile)=47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile,respectively,of PMSD data from EPA's WET Interlaboratory
Variability Study(USEPA,2001a;USEPA,2001b). The lower PMSD bound represents a practical limit to the sensitivity of the test method and is
not a minimum acceptance criteria.
USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-B-01-004 and EPA-821-B-01-005.US
Environmental Protection Agency,Cincinnati,OH.

} Entered by:
Kelley Keenan
Statistical Analyses Reviewed by:
Jim Sumner
Environmental Testing Solutions,Inc_
a
Ceriodaphnia Survival and Reproduction Test-Reproduction
Start Date: 12/9/2020 Test ID: CdPFCRNC Sample ID: Andrews
Ent Date: 12/16/2020 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sa-nple Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
Comments:
Conc% 1 2 3 4 5 6 7 8 9 10
D-Control 26.000 25.000 28.000 27.000 27.000 25.000 27.000 26.000 28.000 25.000
D-Control 28.000 27.000
13 33.000 31.000 27.000 32.000 31.000 34.000 31.000 31.000 34.000 29.000
13 34.000 35.000
Transform: Untransformed 1-Tailed
Conc% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
D-Control 26.583 1.0000 26.583 25.000 28.000 4.381 12
13 31.833 1.1975 31.833 27.000 35.000 7.316 12 -6.984 2.508 1.885
Kiliary Tests Statistic Critical Skew Kurt
S apiro-Wilk's Test indicates normal distribution (p>0.01) 0.9577 0.884 -0.6303 0.92478
F-if est indicates equal variances(p=0.03) 4 5.31963
H pothesis Test(1-tail,0.01) MSDu MSDp MSB MSE F-Prob df
H noscedastic t Test indicates no significant differences 1.88546 0.07093 165.375 3.39015 5.2E-07 1,22
T atments vs D-Control
Dose-Response Plot
40
35
30
O 25- 1-tail, 0.01 level
of significance
7
-a 20
2
a
• 15
cc
10
5
0 -
O
C
O
0

•
it
, 351 Depot Street
= Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Enviro mental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: >:rth Environmental Services,Inc. NPDES#: NC0020800 Pipe#: 001 County: Cherokee
ndrews WWTP Purchase order:
Species: i 'riodaphnia dubia Effluent dilution: 13%
Test type: ss/Fail Chronic Parameter code: TGP3B
I
Sample information: (to be completed by sample collector)
i
Composite sample: Sample location: OKG✓fr s
Start C ate: ia'1 ;2D Time: S-CUAJ`1 Volume collected for testing: L;I-e,r
End d pte: l ol)$ 2-0 Time: 70 7 Air Number of containers filled for testing: i i
Pt
i Number of samples per hour: A--Opfp/, I� Method of transport to laboratory: CO a rt 2 r
Chil ed during collection? T ii Yt2 S Comments:
If chi ed,specify temperature: ,°�
Tiple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
f
t
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGN NG BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample:alected by: ,
li
meLc-t, c• t "Ikle I- ,t1 is jai 2.�
i ,r ' ?n7 APA
Print S:gnat Date and time
_ I
Relinqu sled by: Received by:
MOW.,C- 9e44.4 - 4 (1^ (2 f elz.0 M vl C, I/ i z.I S t z c
iiiair
Prim Signattro Data and time Print /' Signature Date and tune
Relinqu)led by: Received by:
d
1 q-i-, t✓
IL>o6.,Z o
Pnnt Signature Date and time Pent Signature Dais and time
Sample ret°ipt information: (to be completed by ETS personnel)
Relinqu 9l ed to ETS by: Received at ETS by:
Mir/Zit - tw at--Lt2
Ch7:1k"C- Cptajod4422a'-'1 1 I HO 7-e-iii'.4"-.ea.:A.,...)i. I2(6 I it-fD
Pnnt Signature Date and time Print Signature Date andtime
Cua:•dy seals intact?: — Q II Sample temperature upon receipt at ETS(°C): `
,,
Yes No Not used ,5 (____.
Samples received in good condition?: icia -
.es No Total residual chlorine upon receipt at ETS: _ Ilin
, (DPD Presence/Absense Indicator,MDL=0.10 mg/L) Prescnt Ab>,mt
Tra a ringP`number: P ,q �
Project numbei Sample number: Vli `�'�"'
/�
Comore tts:

351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
N
Enviro mental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: $i rth Environmental Services,Inc. NPDES#: NC0O2080O Pipe#: 001 County: Cherokee
ndrews WWTP Purchase order:
Species: i •riodaphnia dubia Effluent dilution: 13%
Test type: I ass/Fail Chronic Parameter code: TGP3B
Sample in rmation: (to be completed by sample collector)
Comptc to sample: Sample location: (L-M,NkeA'F-
Start date: I, 21.) Time: 6(,Z)h,,N) Volume collected for testing: j („.;-1-E( 1
End dtte: ` Time: tJ(XDcld`1 Number of containers filled for testing: I f
Number of samples per hour: r-): ti Method of transport to laboratory: 61i ttci e
Chilled during collection? G 5 Comments:
If ch l3 td,specify temperature:
c.
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. I,
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
a
e
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNI VG BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE i
Sample cd ected by:
VYj(zf•k t' 4,it-K. C 1, t_1(ID f
rCa-lk.-f ,/)P"Lk' it goio EtM
Print Signature Date and time
Relinquished by: /' Received by:
p/j)( I ( t l
�7J ;.Am �G-1.0
njoir,,,, 1,21,1}nocium
7
pro `— Signature Date and time Pnnt Signature Date and time
Relinquist id by: Received by:
c
Print Signature Date and time Pnnt Signature Date and time 1
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
/2./10/Z0 (2-0,
)1,'
Pant Signature Date and time Print Signature le and time
Cusloly seals intact?: n - 1 1 Sample temperature upon receipt at ETS(°C): ry u .L
Yes No Nnt used ,�(
Sam p es received in good condition?:
Yes No Total residual chlorine upon receipt at ETS: _
(DPD Presence/Absense Indicator,MDL=0.10 m5/L) Pre,ent Absent
g PS
Tracking number:
Project number ellSample number:ZOI240.idaa
Comment s:

., Ceriodaphnia dubia
j
Chronic Reference Toxicant Control Chart
Environmental Testing Solutions,Inc. Source-. In-house Culture
1.14 I I I I I I I I I I I I I I I I I I I I
1.12 - Control Limits (± 2 Standard Deviations) -
1.10 - ••
• • • • —
• • • �� ,: -•, •
•
•
• -
1.06 •
co —
1.04 — _
Z
1.02 — _
du0 I 1 I I I I I I I I I I I I I I I I I I
V - I .. I I I .. I:. ..1. _..1 .I, I I�_..II... ,..1_, .h I 1, .., I.,...I,_-.1,I . . 1 ...1 -
> 1.15 —
—
RI Warning Limits
• • • •
—
1.00 —
0.95 1 1 1 1 1 1 I 1 1 1 1 I 1 1 1 1 1 I 1 1
e,06 09'10 '0A$ 1'1 OS NI" o'y 0,V 0ti. 01L'0y 03 03 0p.' OS OS 05�S OS' 01 Ol OS OR 0'3" '0 O6' - 1.L 0a�0
Test date
• 7-day IC25=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodaphnia reproduction (calculated using ToxCalc).
----- Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
-- . , Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
,...,,- .:-...-- Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
USEPA Warning Limits (mean logarithmic IC25±SA.lo converted to anti-logarithmic values,
SA 10=10th percentile of CVs reported nationally by USEPA)
Itend and
lint Sumpn

PO Box 7565
Asheville, NC 28802
,.- Phone: [phone]
Environmental Testing Solutions.Inc.
Fax: [phone]
March 15, 2021
Ms. Wanda Ladd
Earth Environmental Services, Inc.
75 Bison Lane
Murphy, NC 28906
ETS Project Number: 15788
Test Start Date: March 03, 2021
Facility Tested: Andrews WWTP
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Parameter Result
Test Procedure, Method Number
Code PASS FAIL
TGP3B North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure v
EPA-821-R-02-013, Method 1002.0 /�
All toxicity testing results required as part of your permit must be entered on the Effluent Discharge
Monitoring Form (MR-1)for the month in which it was performed, using the parameter code TGP3B.
Additionally,the DMR Reporting Form AT-1 must be signed and e-mailed or mailed to the following
address:
ATForms.ATB(c�ncdenr.ciov
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
Please call if you have any questions concerning these results.
Sincerely,
1.:‘
life tuner
Laboratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037,DrinkingWater: 37786,Wastewater: 600
f l; Y
South Carolina Certificate Number: Clean Water Act: 99053-001

PO Box 7565
Asheville,NC 28802
Phone: [phone]
a
Environmental Tenting Solutions,Inc. Fax: [phone]
Effluent Toxicity Report Form -Chronic Pass/Fail and Acute 1..C50 Date: March 15,2021
Facility: Earth Environmental Services,Inc. NPDES#: NC 0020800 Pipe#: 001 County: Cherokee
Andrews WWTP
Laboratory Performing Test: Environmental Testing Solutipns, nc.,Certificate 037 Comments
Signature of Operator in Responsible Charge(ORC): /,/J l
ORC Phone/E-mail: iDti-SS 9-$630 / i.de di.h::fe(JAi1)- africk.JS,?f,tie ii Project#: 15788
Signature of Laboratory Supervisor: q„�up,aQ.`�' Sample#: 210302.19,210304.21
e-Mail to: ATForms.ATBPncdenr.gov
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-Stat/Rank Sum -3.829
Raleigh,NC 27699-1621 1-Tailed Critical 2.508
%Reduction: -10.2
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent Average
Mortality Reproduction
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Control Control
Number of Young Produced 31 28 32 33 28 33 32 30 29 31 29 28 0.0 30.3
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L Treatment 2 Treatment 2
0.0 33.4
Effluent Percentage 13% Control cv
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 6.3 PASS FAIL
Number of Young Produced 35 34 28 34 36 35 34 34 33 32 33 33 "°""°' "`°" °'°""g
3.ano°a
•
Adult Survival: (L)ive,(D)ead L L L L L L 1 L L L L L L 100.0 X
pH(S.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date: March 03,2021
Control 7.56 8.13 7.89 7.69 7.80 7.51
Treatment 2 7.52 7.64 7.94 7.59 7.44 7.49 Collection(Start) Date:
CO a Sample 1 03-01-21 Sample 2 03-03-21
V) W H W N W
D.O.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control 7.7 7.8 7.6 7.9 7.7 7.6 Grab Comp. Duration
Treatment 2 8.0 7.9 7.8 8.1 7.7 7.6 Sample 1 • X 23.5-h c , (N
o v _v v
Sample 2 . X 22.75-h = ns Q a
LCso/Acute Toxicity Test Alkalinity(mg CaCO3/L) 60,59,58
:Mortality expressed as%,combining replicates.) Hardness(mg CaCO3/L) 87,87,85
Conductivity(µmhos/cm) 320,302,324 203 212
oncentration(%) Total Residual Chlorine(mg/L) <0.10 <0.10
Mortality(%) Sample Temp.at Receipt(°C) 3.7 1.8
LC50= Method of Determination
95%Confidence Limits _Trimmed Spearman Karber Y
CO at cto Probit n W w
Other: control
High Conc.
Organism Tested: Duration: pH(S.U.) DO(mg/L)
DWR Report Form AT-1

Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0,NC Modification—December 2010,Version 3.0)-Control Bench Sheet
Control#: I Date: 03-03-21
Test Grouping Information: Test Organism Information:
Facility Project# Organism Source: In-house Culture
6 F AI.)K .ups t y-? IS IS 5 Age: <24-hours old
5 Li.aJ FFS tb.e QOS iSig Q Source(culture board): O1-1,-y--y,0 jj
4 —SO Q1.1\1,t..t.Q. ,5'141 Replicate# 1 2 3 4 S 6 7 8 9 10 11 12
3 .6e lewLS o0(o A ,S lab Culture board cup# 2 a tt ig tf 201.4 1S Z.z1 1..R
2 AIV 60 S. ►S'►Rk Date and time organisms were born G3-Ub-i_•. 0y.44, -rp tOsp
1-Control between:
Average transfer volume: <0.25 mL
Transfer bowl information: pH(S.U.): 1,(,4
Temperature(°C):ZS•0
Daily Renewal Information:
Test Initiation,renewal,'feeding or Selenastrum YWT Location Randomizing
Day Date termination MHSW Batch
Time Analyst Batch Batch Incubator/Shelf Template
0 03-03-21 Inieacion/Fe5ding
1'1Y\ 02•zs'1.lA cst-ie,-tt of as tI "Lf,3 (rot.
1 03-04-21 V
Feeding
00 lk
2 03-05-21 Renewals/Feeding
0 q DO en!ft.'1.1 C.
3 03-06-21 Feod O p
4 03-07-21 Feedin _g 00 Y,E.V_
5 03-08-21 Renewal2//Feeding
asses A ct .oa•1tA
6 03-09-21 Feemn
®lo30
7 03-10-21 T`ra a;ii?n t
'Organisms fed daily SO uL NC Selenastrum and 50 uL WT per replicate usine HandvSteu repeat Pipet-tor SN 17E59354.
Chemical Analyses: Initiation Renewal One Reneal Two
Initial Final ( Initial ) Final I Initial Iw Final I
Concentration Analyst kr> j�i -/f-}!_S �, i
pH(S.u.) 1.Sto (3 43 j•e J pQ T-4r'9 -,610 1.471
Dissolved oxygen(mg/L) 1n 1.DJ Io -4-9 -4-' 1.t,o
Conductivity(µmhos/cm) 2�o a' 32-
Control,MHSW J -.
'Alkalinity(mg/L CaCO5) ks20 S°t S8
•
°Hardness(mg/L CaCO<) 81 81 $S
*Temperature(°C) Z.q• 1 _tc '`
. 1._ t•41/4 Z.S-l -1.4-4 ZS.t
".Analyst identified for each day.performed pH.dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation.renewal or termination by
the analyst identified in the Daily Renewal Information table. Alkalinity and hardness performed by the analysts indenlified on the lest bench sheets and transcribed to this bench sheet.
Parameter Reporting limit Methoo number Meter Serial number
pH 0.15.U. SM 4500-H+B-2011 Accumet AR20 93312452
Dissolved oxygen 1.0 mg/L SM 4500-0 G-2011 YSI Model 52CE 18D104324
Conductivity 14.9 µmhos/cm SM 2510 B-2011 Accumet AR20 93312452
Alkalinity 5.0 mg CaCO,iL SM 2320 B-2011 Accumet AR20 93312452 !I
Hardness 5-0 mg CeCO,/L SM 2340 C-2011 Not applicable Not applicable
Total residual chlorine 0.1 mg/L ORION 97-70-1977 Accumet AB250 52349123
Temperature 0.1°C SM 2550B-2010 Digital Thermometer )ldle`(b$S
Survival and Reproduction Data:
Day Observations Replicate number
1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality p`
Renewal One (L=Live,D=dead) �"' \`
Number of broods present L `,L [ [ w.. 1
5 Number of young produced IS 1'1 t 8 16, is (5 I1 04 )1 it, 13 12_.
Renewal Two Adult mortality ,� L N \ k— k____ L.
(L=Live,D=dead)
Number of broods present L . Li_ L!,_ LPL L L L\ t_ LA, Li L. L. I t
Number of young produced lb 1%4 I LI r+t I (& IS ao j Z i i(q lip
7 Total young produced 3l 1& 3,L �."3 1 e S,S 3„— N0 ,i° 3\ ZA t6
Final a-O
Final adult mortality L t_� l— k_, l— '
(L=Live,D=dead)
X for 3rd Broods X X X_ x X X 'X— x, X X X X
Control Acceptance Criteria:
%of Male Adults(5 20%) 01, Mean Offspring/Female
%Adults having 3rd Broods(>_80%) /06 1. (>_15 offspring/surviving female) °30•3
%Mortality(5 20%) 01. %CV(<40%) 6.3 T
SOP AT12-Revision 4-Exhibit AT12.3

_.__. Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0,NC Modification-December 2010,Version 3.0)-Test Bench Sheet
Paired with Control# t Date: 0S-$ tl
Client Earth Environmental Services NPDES# NC0020800
Facility Andrews WWTP Outfall 001
Project# iSiCk County Cherokee
Test Concentration(Chronic Limit) 13% Dilution mL mL Total volume
preparation: Sample Dilution water ml
Samples were not aerated or treated unless otherwise noted on this form. Control,dilution water
and test renewal information are included on the Control Bench Sheet indicated above. 39 261 300
Chemical Analyses: initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst ICD KA EP nS /Li kD
pH(S.U-) Z.S-Z 7•(/r-1 -1.94 �.5t i c{4 1.c.(1
Test Dissolved oxygen(mg/L) do -1.cl 7 e,
Concentration Conductivity(µmhos/cm) 1")1\ Z 4C2 3?Li
*Temperature(°C) -LS-1 As. 2 ti-\.q "\S\-‘:\ -LA-7 Z5-1_
pH(S.U.) -1•I $ -1• 31
Dissolved oxygen(mg/L) , $7475-0) 7
100% Conductivity(µmhos/cm) Z 03
2tZ
*Total residual chlorine(mg/L) <0.1 <.0 • 10
Sample number sampler -Z(()3ot 1ii Sample Z10.0`A -ZI
*Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation,renewal or termination by
the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual
Chlorine Bench Sheet and transcribed to this bench sheet.
Survival and Reproduction Data (performed at test concentration):
Da Observations Replicate number
y 1 2 3 4 5 6 7 8 9 I 10 11 12
2 Adult mortality
Renewal One (L=Live,D=dead) •__ y` `- \_"" `" \-. V_
Number of broods present I= L. [ Li LI_ L2 2 it_ I
5 Number of young produced 2_0 l8 E� L° 19. Li Zl lo 11 2 p t& 1 8�,
Renewal Two Adult mortality L \v L \_. I_
(L=Live,D=dead)
Number of broods present I— b. L LI._ Li L Lt I i >t Li_ 11_
Number of young produced 1S I(o IS I' j I, t. t`-1 tl I . t� S t 1 5
7 Total young produced
Final 'SS 3`-1 156 3`\ 3S 3a 3`A IS 31 33 33
Final adult mortality
(L=Live,D=dead) �"' �..- L-- L k_ k__. %—
Test was initiated using Sample 1. Sample 2 was used for Renewals One(day 2)and Two(day 5). Samples were diluted to the test concentration prior to use with moderately hard
synthetic water and warmed to 25.0±1.0°C in a warm water bath.
Comments:
Test Results and Statistical Analyses:
Test results Statistics
Mortality 07 t-Stat or
Rank Sum - 3.cc2i
Mean offspring 1-Tailed
per female 33'-1 Critical Z-SOS
%Reduction n S
from control
10.11. PASS or FAIL I'
SOP AT12-Revision 4-Exhibit AT12.2

s
A
-i
1
, Environmental Testing Solutions,Inc.
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia
Cmered by:
Facility: Andrews WWTP Kelley Heenan
Reviewed by:
Test start date: March 03,2021 Jim S. 1er
Project number: 15788
154
Sample numbers: 210302.19,210304.21
—
Concentration
Number of young produced by replicate number Survival Average Coefficient of Percent reduction from
Day (%) reproduction variation (%) control(%)
(ie) 1 2 3 4 5 6 7 8 9 10 11 12 (offspring/female)
5 15 14 18 16 15 15 17 14 17 16 13 12
Control 7 16 14 14 17 13 18 15 16 12 15 16 16 100 30.3 6.3 Not applicable
Total 31 28 32 33 28 33 32 30 29 31 29 28
5 20 18 14 19 19 18 21 20 17 20 18 18
13% 7 15 16 14 15 17 17 13 14 16 12 15 15 100 33.4 6.0 -10.2
Total 35 34 28 34 36 35 34 34 33 32 33 33
Dunnett's MSD value: 2.02 MSD= Minimum Significant Difference
PMSD: 6.7 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10th percentile) =13%.
Upper PMSD bound determined by USEPA(90th percentile)=47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile,respectively,of PMSD data from EPA's WET Interlaboratory
Variability Study(USEPA,2001a;USEPA,2001b). The lower PMSD bound represents a practical limit to the sensitivity of the test method and is
not a minimum acceptance criteria.
USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-B-01-004 and EPA-821-8-01-005.US
Environmental Protection Agency,Cincinnati,OH.

Entered by:
Kelley Keenan
StatiStatistical Analyses Reviewed by:
stical` Jim Sumner
Environmental Testing Solutions,Inc. ®,
Ceriodaphnia Survival and Reproduction Test-Reproduction
Start Date: 3/3/2021 Test ID: CdPFCRNC Sample ID: Andrews
d Date: 3/10/2021 Lab ID: ETS-Envir.Testing Sol. Sample Type: DMR-Discharge Monitoring Report
mple Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
mments:
Conc-% 1 2 3 4 5 6 7 8 9 10
D-Control 31.000 28.000 32.000 33.000 28.000 33.000 32.000 30.000 29.000 31.000
D-Control 29.000 28.000
13 35.000 34.000 28.000 34.000 36.000 35.000 34.000 34.000 33.000 32.000
13 33.000 33.000
Transform: Untransformed 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
D-Control 30.333 1.0000 30.333 28.000 33.000 6.339 12
13 33.417 1.1016 33.417 28.000 36.000 6.047 12 -3.829 2.508 2.020
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution(p>0.01) 0.93177 0.884 -0.8665 1.19262
I-- est indicates equal variances(p=0.87) 1.10451 5.31963
Hypothesis Test(1-tail,0.01) MSDu MSDp MSB MSE F-Prob df
hdrnoscedastic t Test indicates no significant differences 2.01972 0.06658 57.0417 3.89015 9.1E-04 1, 22
T-6atments vs D-Control
Dose-Response Plot
40
35
30 1-tail, 0.01 level
of significance
O 25
• 20
2
re• 15
10
5
0
o
C
O
U
0

gO
V 351 Depot Street
' 4" Asheville,NC 28801
Phone: [phone]
Fax: [phone]
_ Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Earth Environmental Services,Inc. NPDES#: NC0020800 Pipe#: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 13%
Test type: Fass/Fail Chronic Parameter code: TGP3B
Sample it ormation: (to be completed by sample collector)
;i Comp 'te sample: Sample location: :_,faaK."e'✓l
Start ate: Time: Q�j!�/t Volume collected for testing: ( `r T
End ate: ,t, Time: ,72t,,,qni Number of containers filled for testing: /
Nurc Br of samples per hour: p,7j % Method of transport to laboratory:
'
111 i p rY �ou:"I
Chilll d during collection? (, (✓S Comments: e
o
If chflkd,specify temperature: /�(� / o
r ple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. t
i
Sample c siody: (to be completed by sample collector and facility personnel)
BY SIG NG BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample I ected by:
t marl e J ✓,Z/z/
Signature Date and time
Relinqui4htd Print
by: , Received by:
� 3,)21C-7 i ,----37--)il 4 L'.c&i).e4,1 —.. .....,..- Lf/2"/7-1 1 y1 ' 4"
Pri t Signatur�����Vlllll Date and time Print ,if Signature Date and time
Relinquisl'd by: Received by:
Print._.. .. ......._ .. ...... .......
R Signature Date and time Pnne Signature Date and time
,itt umwxrtr •,nra:..,.,.., ...,...,..... ,.. ._.K......,.,w.,x.:•,,ID,-.,M.,.,..�•,.,,, x �ssccr�.sa ^a,eaczs:.�.xasa�w-,rn«usysa�a�mz••.,rmF."s:s•:r
Sample ret pt information: (to be completed by ETS personnel)
1
Relinquis atcl to ETS by: Received at ETS by:
3i-z_ rZI
Print Signature Date and time Print Signature Date and time
Custkxy seals intact?: - 1-1 ® Sample temperature upon receipt at ETS(°C): r Ti
Yes No Not used j C.
Samples received in good condition?: iss NE
Yes No Total residual chlorine upon receipt at ETS: I 11
` (DPD Presence/Absense Indicator,MDL=0.10 mg/L) Pre"nt At"eo
Trac •tg number: N
Project number:Ij 3 Sample number: �v�j(2,,1
Comme :

:r, 351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environrnental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: arth Environmental Services,Inc. NPDES#: NC0020800 Pipe#: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Ceriodaphnia dubia Effluent dilution: 13%
Test type: Pass/Fail Chronic Parameter code: TGP3B
) Sample information: (to be completed by sample collector) ��',
Composite sample: Sample location: e-fli t,wei
j Start c ate: J/3a I l 1 i- r
_ ./ Time: Fj Alin Volume collected for testing: 1
End date: Time: Cj 4'jtq Number of containers filled for testing: / f
Nun•b er of samples per hour: /�pp(p;. /� Method of transport to laboratory: eto“r'€ -
Chil ed during collection? r ll,�e) Comments:
If chi led,specify temperature: tie)V e t
"It ple rinse sample container with sample before filling. Completely fill the sample container with no air space. r
l Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. n
4
Sample c si ody: (to be completed by sample collector and facility personnel)
I
BY SIG 1AJG BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample., ected by:
illt2 I e. 1406/(7 3/171/2(
9 Yeif.lY&,9>11I
Prntl Signature• Date and time
1 Relinqui• ed by: f: Received by: 1
tY,. 0, ' 3/1//2 i •SAO (I` awn �.. �4�z
Signature Date and time Print "OfSignature Date and time
Relinqui• ed by: Received by:
LI
Print Signature Date d time Print Signature Date d urne
Sample re, pt information: (to be completed by ETS personnel)
Relinquis d to ETS by: Received at ETS by:
V � i /y/t1 `� vt �� Z i
Eo�.� IZ:23'tM U —
Print Signature Date and rime Print Signature Date and time
Cust y seals intact?: _ - Sample temperature upon receipt at ETS(°C):
Yes No Not uttd ' a (.
Samples received in good condition?:
Yes No Total residual chlorine upon receipt at ETS: _ _
(DPD Presence/Absense Indicator,MDL-0.10 maiL) P"`em Absent
Trac 'tg number: 0e -�D�
Project number:(598t,+Sample number: A.g V7-24
Comme

Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
Environmental Testing Solutions,Inc. Source: In-house Culture
1.14 1 I I I I I I I I I I I I I I ! I I I 1
1.12 - Control Limits (± 2 Standard Deviations) -
•
1.10 - • • -
• • ••
•
•
•
_„...._—_____—_.—
• _•
•
1.06
-
m 1.04 - _
Z
1.02 - _
btl I I I I I I 1 I I I I I I I I I I I I I
in
V - I I I I I .I..,_,,.,.,I
>. 1.15 - -
o Warning Limits
• •
•
•
0.95 I I I 1 1 I 1 1 1 I 1 1 1 1 1 I 1 1 1 i
11"0'�01�l 01�l 01"A�61.- 0'�03 0A"01 oS"05�5A5"06- 01.01 og OQ"09- 1p.06"1)-03 sl.pa 01 p5 01,p2 01: 03�2�1
Test date
• 7-day IC25=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodaphnia reproduction (calculated using ToxCalc).
x--.•-•— Central Tendency(mean logarithmic ICZ5 converted to anti-logarithmic values)
- ., .. - — Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
=:M..,,e—m. Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
••__ USEPA Warning Limits (mean logarithmic IC25±SA.10 converted to anti-logarithmic values,
SA 10=10th percentile of CVs reported nationally by USEPA)
nteted
eel/mewed by
nm Sumner
a(

PO Box'7565
Asheville, NC 28802
Phone: [phone]
Environmental Testlng Solutions,Inc.
Fax: [phone]
June 23, 2022
Ms.Wanda Ladd
Earth Environmental Services, Inc.
75 Bison Lane
Murphy, NC 28906
ETS Project Number: 17053
Test Start Date: June 15,2022
Facility Tested: Andrews WWTP
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Result
ParCodeter Test Procedure, Method Number e PASS FAIL
North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure v
TGP3B EPA-821-R-02-013, Method 1002.0 /�
All toxicity testing results required as part of your permit must be entered on the Effluent Discharge
Monitoring Form (MR-1)for the month in which it was performed, using the parameter code TGP3B.
Additionally,the DMR Reporting Form AT-1 must be signed and e-mailed or mailed to the following
address:
ATForms.ATBncdenr.qov
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
Please call if you have any questions concerning these results.
Sincerely,
im umner
Laboratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001

,
x
PO Box 7565
Asheville, NC 28802
Phone: [phone]
Environmental Testing So!toins,inc. Fax: [phone]
Effluent Toxicity Report Form- Chronic Pass/Fail and Acute LC50 Date: June 23,2022
:acility: Earth Environmental Services, Inc. NPDES#: NC 0020800 Pipe#: 001 County: Cherokee
Andrews WWTP
Laboratory Performing Test: Environmental Testing Solu�s, Inc.,Certificate Comments:
Signature of Operator in Responsible Charge(ORC): .4:2 - 1A- .
0<C Phone/E-mail: 9 - 57-gi,3b /,�,�o {t*er kni-6ipydre,;_$is v a c3. Project#: 17053
Signature of Laboratory Supervisor: r a4P-41"/ Sample#: 220614.06,220616.03
e-Mail to: ATForms.ATB@ncd r.gov
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-Stat/Rank Sum -0.103
Raleigh,NC 27699-1621 1-Tailed Critical 2.508
% Reduction: -0.3
Percent Average
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
Mortality Reproduction
CIf ntrol Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Control Control
Nmber of Young Produced 34 29 31 28 30 28 29 29 29 28 27 31 0.0 29.4
Ak ult Survival: (L)ive,(D)ead LLLLLLLLLL L L Treatment 2 Treatment 2
0.0 29.5
I
E`=luent Percentage 13% Control Cy
T-eatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 6.4 PASS FAIL
Number of Young Produced 26 30 28 27 28 33 31 30 31 32 29 29 conrol organisms producing
3rd brood •
v
Adult Survival: (L)ive,(D)ead LLLLLLL L L L L L 100.0 ^
H(S.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date: June 15, 2022
Control 7.61 7.78 7.40 7.75 7.58 7.70
Treatment 2 7.60 7.98 7.49 7.76 7.64 7.82 Collection(Start) Date:
-, Sample 1 06-13-22 Sample 2 06-15-22
C C C
Le, W N W N W
3.0.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control 7.6 7.8 7.7 7.9 7.8 7.6 Grab Comp. Duration
Treatment 2 7.6 7.9 7.9 8.0 7.9 7.6 Sample 1 . X 22.5-h = ry
a v al CD
Sample 2 . X 23 5-h ? i 2 2
0 N n
LC50/Acute Toxicity Test Alkalinity(mg CaCO3/L) 60
Mortality expressed as%,combining replicates.) Hardness(mg CaCO3/L) 85
Conductivity(µmhos/cm) 310,298,298 292 305
QlmIcentration(%) Total Residual Chlorine(mg/L) <0.10 <0.10
IV xtality(%) Sample Temp.at Receipt(°C) 3.3 1.5
LC50= Method of Determination
95%Confidence Limits `Trimmed Spearman Karber -
W W
to Probit N
Other: Control I
High Conc.
Q-ganism Tested: Duration: pH(S.U.) DO(mg/L)
i
UWR Report Form AT-1

t
Page 1 of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0,NC Modification-December 2010,Version 3.0)-Control Bench Sheet
Control#: k Date: 06-15-22
Test Grouping Information: Test Organism Information:
Facility Project# Organism Source: In-house Culture
6 Pi Alt, lAtL OO'L iloSi Age: <24-hours old
5 Fdia,Al.a4.LI IyJ ta.l P I`t 0 SS Source(culture board): DI,-VI.tt 6
4 }o W.r'P no% Replicate# 1 2 3 4 t 5 6 7 8 9 10 11 12
3 C 0LV N$JG3 CI Ole Culture board cup# S 1 a tl tt 13 l$l 34}'. al 2ae
2 A tol®ft.e.,)S t•t0S'\ Date and time organisms were born 0(0_i 6.Zi 0600 To Ito 0
1-Control between:
Average transfer volume: <0.25 mL
Transfer bowl information: pH(S.U.): .1,e'1
Temperature('C). •): -t
Daily Renewal Information:
Test Initiation,renewal,'feeding or selenastrum YWr Location Randomizing
Day Date termination MHSW Batch •
Time Analyst Batch Batch Incubator/Shelf Template
0 06-15-22 'ni tiore e ng ndi
�i� Yl 061ii•1. 06 uz Lt ova vc 1z 141- traee 1.1
1 06-16-22 fae0 0 A•(
2 06-17-22 Renewal 1/Feeding 1' L ,�4 �♦
a�vV ��S 1/r OO Y'�•u.
3 06-18-22 '9er9Jt f
• 4 06-19-22 Feeemg
a9 No TCr
I 5 06-20-22 Room ending 0,-o,o.t.is
6 06-21-22 r di'g
0 f5® A.(
erminatZion /
7 06-22-22 a'1 -'>
•Organisms fed daily 50 uL NC 5eleenastrum and 5U4L YWT per replicate using RandvStep repeat pipettor SN 17E99354.
Chemical Analyses: Initiation Renewal One Renewal Two
�Initiial Final Initial Final Initial Final
Concentration Analyst '• A.( ,4.g & "C(S'/ ffi
/1Vv
pH(S.U.) /1 GI 7.78 a.6/O "1.AS 7 5 - -1 --
Dissolved oxygen(mg/L) ki —e-& -N.-I, 1A -1. -1.(o
Conductivity(µmhos/cm) 319
298' Zq •
Control,MHSW - -
'Alkalinity(mg/LCaC0a) b
`Hardness(mg/LCaCO,) 0�1';-�•,'�
'Temperature(ec) --LA Jo AAA 1}1-'1 Z•{,al IA.4 AS.\
'Analyst identified for each day.performed pH,dissolved oxygen and conductivity measurements only.Temperatures performed at the time of test initiation.renaval or termination be the
analyst Identified in the Daily Renewal Information table.Alkalinity and hardness performed by the analysts indentificd on the test bench sheets and transcribed to this bench sheet
Parameter Reporting limit Method number Meter Serial number
pH 0.1 S.U. SM 4500-H+B-2011 Accumet AR20 933:2452
Dissolved oxygen 1.0 mg/L SM 4500-0 G-2016 Y51 Model 52CE 180104324
Conductivity 14.9 pmhos/cm SM 2510 B-2011 Accumet AR20 93312452
Alkalinity 5.0 mg Cc COOL SM 2320 B-2011 Accurnet AR20 93312452
Hardness 5.0 mg CeCO3/L SM 2340 C-2011 Not applicable Not applicable
Tout residual chlorine 0.1 mg/L ORION 97-70-1977 Accumet AB250 92349123
Temperature 0.1"C SM 2550B-2010 Digital Thermometer 130.6(0,..1 °
Survival and Reproduction Data:
Day Observations _ Replicate number
1 I 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality I
Renewal One (L=Gve,D=dead) t_ L_ t_ L_
Number of broods present Ltd L i L . Lt L b Li. Li 1-
5 Number of young produced 61 L`O t IL: 1 1 1 ` e 1� ! ,
Renewal Two Adult mortality
• (L=Live,D=dead) L_'" L__ `- '-L I.— L. `' l_ � `�
Number of broods present L L L L1 L 1 !� L__I It L1 L\ L l L,-.
Number of young produced ii It is iy R. It 1�p I/. I� 11 I_
7 Total young produced `' c 1 1t g -4G c 11 3,
Final .�."0 -1..1 Jt Z� .TJ �. 2�R \ L"1 8
Final adult mortality L_ L 1r L l_ L L
(L=Live,D=dead) .1
X for 3rd Broods x >( �. /N , i( )1 2< / x
Control Acceptance Criteria: 1
%of Male Adults(<_20%) 0 7 Mean Offspring/Female t�
OG 5 15 offspring/surviving2.1• 1
%Adults having 3rd Broods(>_SOY) / �� (- female)
%Mortality(<20%) O 1, %CV)<40%) to,LI 1 y
SOP AT12-Revision 9-Exhibit AT12.3

1
•Remer.:_ew
Page l of 1
North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test,Species: Ceriodaphnia dubia
(EPA-821-R-02-013 Method 1002.0, NC Modification-December 2010,Version 3.0)-Test Bench Sheet
Paired with Control# I Date: Ole-1S-'11-
' Client Earth Environmental Services NPDES# NC0020800
Facility Andrews WWTP Outfall 001
Project# 1-kOS County Cherokee
Test Concentration(Chronic Limit) 13% Dilution mL mL Total volume
preparation: Sample Dilution water mL
Samples were not aerated or treated unless otherwise noted on this form. Control,dilution water
and test renewal information are included on the Control Bench Sheet indicated above. 39 261 300
Chemical Analyses: Initiation Renewal One Renewal Two
Initial Final Initial Final Initial Final
Concentration Analyst 1t/v 4.r ,q,j " -vs- til Y- _
pH(S.U.( t
7Ga 7 9� 7 `�9 7.16 7, 6q 1.C2.. ""_G4.gre.
Test Dissolved oxygen(mg/L) 7(i 1_' -1_4, g.0 "IA -1_
Concentration Conductivity(pmhos/cm) 310 30•r- 30-I_
*Temperature(°C) -LS- 1 'LS•\ 1..`t•1 -LS-L •X.A.i 1.S•1
pH(S.U.) -j 3°1 7.33
Dissolved oxygen(mg/L) 6. s-0
100% Conductivity(µmhos/cm) Q-1.Q .3®J—
*Total residual chlorine(mg/L) C p , I 0 4- 0 * t 0
Sample number Sample 1 -1-Ir060y_b\a Sample 2 ...L.L0(0 1L
*Analyst identified for each day,performed pH,dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation,renewal or termination by
the analyst identified in the Daily Renewal information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual
Chlorine Bench Sheet and transcribed to this bench sheet.
Survival and Reproduction Data (performed at test concentration):
I Observations Replicate number
Day —1 2 3 4 5 6 7 8 9 10 11 12
2 Adult mortality
Renewal One (L=Live,D=dead) 1,.._ t-- `_ tr L•' 1_ t __ (✓ L___
Number of broods present b LI: Lam- L L LL La L L It t L1
5 Number of young produced 1 2 l.0 1 I. 1'1 1 S 1, ►S l i L to 14 t 6 t S
Renewal Two Adult mortality L. 1/4---- L- ` L- k L_ L.
(L=Live,D=dead)
Number of broods present lr Li. L1 I_` L1 Li Li Li_ 1 .1_ t_ L_ i_ .
Number of young produced
I), I4 1-1 I° I Ito I6 it IS ig II 1
Final Total young produced 'fib 30 2 t 1..1 /t 35 W .30 .l1 32 - 29
Final adult mortality 1
14=Live,D=dead) 1_. '1/4— \..� V-.. \- L- L- I
Test was initiated using Sample 1. Sample 2 was used for Renewals One(day 2)and Two(day 5). Samples were diluted to the test concentration prior to use with moderately hard
synthetic water and warmed to 25.0±1.0°C in a warm water bath.
Comments: �y
1` MICA'S I11QP,eIL Ut lt�elk,L.Z-;•`k
Test Results and Statistical Analyses:
Test results Statistics
Mortality D. t-Stat or
Rank Sum 0•I0 3
+Mean offspring 1-Tailed
per female ��-5 Critical 2-S 0
Reduction PASS or FAIL PASS
from control , �' 7
SOP AT12-Revision 4-Exhibit AT12.2
I

xt, ,.-
EnvironmentalTesting Solutions,Inc.
Quality Control: Verification of Data Entry, Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia
rntered by:
Kelley Ileenan
Facility: Andrews WWTP Reviewed by:
Test start date: June 15,2022 lint;tuner
Project number: 17053 di'
Sample numbers: 220614.06,220616.03
Number of young produced by replicate number Survival Average Coefficient of Percent reduction from
Concentration (%) reproduction variation (%) control (%)
(/o) Day 1 2 3 4 5 6 7 , 8 9 10 11 12 (offspring/female)
5 17 18 16 14 16 17 13 17 15 14 16 14
Control 7 17 11 15 14 14 11 16 12 14 14 11 17 100 29.4 6.4 Not applicable
Total 34 29 31 28 30 28 29 29 29 28 27 31
5 15 16 16 17 13 17 15 19 16 14 18 15
13% 7 11 14 12 10 15 16 16 11 15 18 11 14 100 29.5 7.0 -0.3
Total 26 30 28 27 28 33 31 30 31 32 29 29
Dunnett's MSD value: 2.024 MSD= Minimum Significant Difference
PMSD: 6.9 PMSD= Percent Minimum Significant Difference
PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared
statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10th percentile) =13%.
Upper PMSD bound determined by USEPA(90th percentile)=47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile,respectively,of PMSD data from EPA's WET Interlaboratory
Variability Study(USEPA,2001a;USEPA,2001b). The lower PMSD bound represents a practical limit to the sensitivity of the test method and is
not a minimum acceptance criteria.
USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-B-01-004 and EPA-821-B-01-005.US
Environmental Protection Agency,Cincinnati,OH.

•
Entered by:
Kelley Keenan
Statistical Analyses Reviewed
Urn Sumner
Environmental Testing Solutions.Inc
Ceriodaphnia Survival and Reproduction Test-Reproduction
S:art Date: 6/15/2022 Test ID: CdPFCRNC Sample ID: Andrews
End Date: 6/22/2022 Lab ID: ETS-Envir.Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia
Comments:
Conc-% 1 2 3 4 5 6 7 8 9 10
D-Control 34.000 29.000 31.000 28.000 30.000 28.000 29.000 29.000 29.000 28.000
D-Control 27.000 31.000
13 26.000 30.000 28.000 27.000 28.000 33.000 31.000 30.000 31.000 32.000
13 29.000 29.000
Transform:Untransformed 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD
D-Control 29.417 1.0000 29.417 27.000 34.000 6.394 12
13 29.500 1.0028 29.500 26.000 33.000 7.007 12 -0.103 2.508 2.024
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution(p>0.01) 0.96357 0.884 0.53533 0.20292
PI-Test indicates equal variances(p=0.76) 1.20771 5.31963
Hypothesis Test(1-tail,0.01) MSDu MSDp MSB MSE F-Prob df
I-omoscedastic t Test indicates no significant differences 2.02365 0.06879 0.04167 3.9053 0.91867 1, 22
Treatments vs D-Control
Dose-Response Plot
40
35
30 1-tail,0.01 level
c 25- - of significance
t)
'36 20
O
15
10
5
0
o �
0
U
0

s� 351 Depot Street
\• Asheville,NC 28801
Phone: [phone]
Fax: [phone]
_ Environmental Testing Solutions,Inc.
1
Whole Effluent Toxicity Chain-of-Custody Form
Facility: ,arth Environmental Services,Inc. NPDES#: NC0020800 Pipe#: 00I County: Cherokee
Andrews WWTP Purchase order:
Species: eriodaphnia dubia Effluent dilution: 13%
Test type: ass/Fail Chronic Parameter code: TGP3B
Sample in ormation: (to be completed by sample collector)
C
1: Composite sample: Sample location: L-11y( t.-
r. Start ci ite: l.vl Mjla Q Time: Jf.I ANA Volume collected for testing: I 1,-,.; 1-e 12
End date: L,1141 Time: A( Number of containers filled for testing: $40- j
Number of samples per hour: AR;C3t. (Li- Method of transport to laboratory: C.oU( 'i e_I
Chill el during collection? Uey5 Comments:
g. If chi ed,specify temperature: ; ('
T ale rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
f
Sample cus xdY• (to be completed by sample collector and facility
personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
4 Sample c.'cted by: �/ t
1 Pilaf1i t . Qe.,1'-!e .Gf4G.4 c, (:01 ii as f,
c lYr to &.3,R
me Pont Signature pate and Lima
Relinquis sed by: - Received by: is
mCt aar . �rc1y�f �:)141aa 3chn C: �J J 1
CI 1/,'ddi—t :r t 3 07. a,Q14 I - _i:>� a7 :3 a
I Poe so— it Date and time Pant Sienatur< and time
Relinquis sped by: Received by: I
I n
Print Signature Date and time .not Signature Date and time
araR
Sample receipt information: (to be completed by ETS personnel)
1
Relinquisltd to ETS by: Received at ETS by:
ti,„„ �jrot..y. L 5
Prim signature Date and time rnm �/ Signature Date and time
Custxly seals intact?: _ _ 1 1 Sample temperature upon receipt at ETS(°C):
Ye. No Not used 'I.'S%C
Samples received in good condition?: l�''�
Yea l No Total residual chlorine upon receipt at ETS: 1-7'
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) Pre'cm Abaent
Tracking number: 0 F"s
Project numbeiP: i Uy Sample number: Qlk(p(4,t!"
Comme ills:
11

351 Depot Street
r A=21 2,\ Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Enviro mental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: I .rth Environmental Services,Inc. NPDES#: NC0020800 Pipe#: 001 County: Cherokee
ndrews WWTP Purchase order:
Species: I -riodaphnia dubia Effluent dilution: 13%
Test type: <ss/Fail Chronic Parameter code: TGP3B
Sample in F rmation: (to be completed by sample collector)
Comp'4 to sample: Sample location: erf t„t._,14
Start tte: 4.71 f 5 22_ Time: '2C0{}/Vl Volume collected for testing: 1 1-.Is-{--r
End ate: (//4 12. _ Time: 1234M Number of containers filled for testing: 1 I
Num er of samples per hour: j 11�Q^ ., (4 �' (Method of transport to laboratory: COu. (
Chill 4 during collection? t o Comments:
If ch �d,specify temperature: 4 e C
T vie rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
a
Sample cu Uody: (to be completed by sample collector and facility personnel)
BY SIGN NG BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample cli,ected by:
Inckr 1.. c? , /AP. (11-, &it 02_2_ .!
Pnn a
Signatwe y Date and time
Relinquis l d by: / 9 Received by:
(IV-if C C. aAR: t1�/2Z .3-3h r1 _ 1r�(t(v/7z I
ir Print v Signature Date and time Print Signature Date and time
Relinquied by: Received by:
ggggg �( - - _ ----se' i
Print Signature Date and time Pant Signature Date and tune
in
Sample rec e pt information: (to be completed by ETS personnel)
Relinquis tied to ETS by: Received at ETS by:
,...fr:t....,..e.y)rsua..._ I 6/tCr/2 //1 LL ut 0(0.110,24
� i7:,40-Wk tL,iD
Print Signature Date and time Print Signature. Date and time
Custer y seals intact?: - - Sample temperature upon receipt at ETS(°C): 1 aS-(
Yen No Not us r1
Samples received in good condition?:r7 -
Yes No Total residual chlorine upon receipt at ETS: 1=1 EE/
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) P1Cien Awn'
Tracking number: IJ 11
Project number:n053 Sample number: 2 O(O D 63
Commedts:

Ceriodaphnia dubia
Chronic Reference Toxicant Control Chart
, Environmental Testing Solutions,Inc. Source: In-house Culture
1.14 I 1 1 I I I 1 1 I 1 1 I I I 1 I I I I
1.12 - Control Limits (± 2 Standard Deviations) -
•
•
• • • • -
•
1.08 - • , ... - -
-
ra 1.04 - -
Z - -
11.02 - -
to I I I 1 I I I I 1 1 I I I I I I I I I I
n�i 1..,.,.L,,,.b., ..d,..,.1,... �.,.....1._.„i— I I I I I r. .. 1--- ..i
V_
>. 1.15 -
ro Warning Limits
•
1.05 -
0.95 1 I 1 1 1 1 1 I 1 1
Oy Oti 01 OS 0-oa O6 Dy ob.- °01,1QOS- 09.1a 091A�1-05 1V 11; O ON' 1: 01 OS- 3 0$Op 0`�oy 03 0(3- �2
Test date
• 7-day IC25=25%inhibition concentration. An estimation of the sodium chloride concentration
which would cause a 25%reduction in Ceriodaphnia reproduction (calculated using ToxCalc).
- Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
..0 -_ — Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
—.W..... . Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
,• USEPA Warning Limits (mean logarithmic IC25±SA.io converted to anti-logarithmic values,
SA.10= 10th percentile of CVs reported nationally by USEPA)
oceree and
liet
Reviewed M
Jim Sumner

PO Box 7565
Asheville, NC 28802
Phone: [phone]
Environmental Testing Solutions Inc.
Fax: [phone]
4tober 31, 2022
`s. Wanda Ladd
E: rth Environmental Services, Inc.
Bison Lane
jurphy, NC 28906
E ;5 Project Number: 17295
T-at Start Date: October 04, 2022
Fcility Tested: Andrews WWTP
Eiclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
PIa ra meter
Code Test Procedure, Method Number Result (ChV)
HP6C Fathead minnow, Pimephales promelas,Larval Survival and Growth Chronic
EPA-821-R-02-013, Method 1000.0 < 3.25%
A I toxicity testing results required as part of your permit must be entered on the Effluent Discharge
rVlonitoring Form (MR-1) for the month in which it was performed, using the parameter code THP6C.
AJJitionally, the DMR Reporting Form AT-5 must be signed and e-mailed or mailed to the following
address:
ATForms.ATB(a�ncdenr.gov
INorth Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
{ 1621 Mail Service Center
Raleigh, NC 27699-1621
PI ,ase call if you have any questions concerning these results.
Sir!:erely,
04
Jir> umner
LE boratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001

to f PO Box 7565
Asheville,NC 28802
• Phone: [phone]
Fax: [phone]
! , Environmental Testing Solutions,Inc.
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date: October 31,2022
Facility: Earth Environmental Services,Inc. NPDES#: NC 0020800 Pipe#: 001 County: Cherokee
Andrews WWTP
Laboratory Performing Test: Environmental Testing Solutions,Inc.,Certificate#037 Comments:Pathogenic infection observed
Signature of Operator in Responsible Charge(ORC): L" L�o-10- on minnows in effluent concentrations.
ORC Phone/E-mail: lag-651 Ja0/ awed&terf0(onfiPUftobndfnC,dr-9 Project#: 17295
Signature of Laboratory Supervisor: Sample#: 221003.01,221005.33,221007.02
e-Mail to: [e-mail] Test Organisms: Pimephales promelas
I Or Mail Original to: North Carolina Division of Water Resources Supplier
Water Sciences Section/Aquatic Toxicology Branch In-house Culture
1621 Mail Service Center Begin hatch: 10-03-22 1445
Raleigh,NC 27699-1621 End hatch: 10-04-22 0500
I
I Test Start Date: October 04,2022
Replicate number
1 2 3 4
Control Surviving number of larvae 10 10 10 10 Survival(%) 100.0
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.886
I Weight/original(mg/larvae) 0.852 0.847 0.932 0.911 Average wt/ 0.886
surviving(mg)
l %Effluent Surviving number of larvae 7 8 5 1
3.25% Original number of larvae 10 10 10 10 Survival(%) 52.5
Weight/original(mg/larvae) I 0.399 0.527 0.255 0.088 Average wt(mg) 0.317
L
I %Effluent Surviving number of larvae 6 4 7 6
1 6.5% Original number of larvae 10 10 10 10 Survival(%) 57.5
Weight/original(nig/larvae) 0.358 0.234 0.414 0.376 Average wt(mg) 0.346
%Effluent Surviving number of larvae 6 3 6 4
13% Original number of larvae 10 10 10 10 Survival(%) 47.5
Weight/original(mg/larvae) 0.356 0.183 0.323 0.219 Average wt(mg) 0.270
%Effluent Surviving number of larvae ! 7 6 6 7
I 26% Original number of larvae 10 10 10 10 Survival(%) 65.0
Weight/original(mg/larvae) 0.438 0.354 0.422 0.430 Average wt(mg) 0.411
%Effluent Surviving number of larvae_ 9 9 8 5
52% Original number of larvae 10 10 10 10 Survival(%) 77.5
Weight/original(mg/larvae) 0.588 0.596 0.559 0.380 Average wt(mg) 0.531
Water Quality Data
Day 0 Day l Day 2 Day 3 Day 4 Day 5 Day 6
Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial l Final
Control
pH(SU): 7.51 6.96 7.18 6.77 7.56 6.96 7.33 6.93 7.64 7.08 7.61 6.98 7.61 7.07
DO(mg/L): 7.6 7.7 7.7 5.9 7.8 7.4 7.6 7.3 7.7 6.5 7.7 7.3 7.7 6.0
Temp.(°C): 24.8 24.4 24.7 24.3 24.8 24.6 24.8 25.1 24.7 24.8 24.8 24.7 24.7 24.6
High Concentration
pH(SU): 7.21 7.09 7.19 6.83 7.10 6.93 7.03 6.92 6.90 7.06 7.40 7.06 7.31 7.07
DO(mg/L): 7.7 7.9 8.0 5.3 7.7 7.7 8.1 6.8 7.5 6.6 7.6 7.2 8.0 5.4
Temp.('C): 24.6 24.4 24.8 24.7 24.9 24.5 24.7 24.9 24.8 24.8 24.9 24.6 24.9 24.9
i
I Sample Information Sample 1 Sample 2 Sample 3 Control Analyses Survival Growth
i
Collection start date: 10-02-22 10-04-22 10-06-22 `. Normal: Yes Yes
Grab: . (Rr` Horn.Var. Yes Yes
Composite duration: 24-h 24-h 24 25 h ,' ;,_ n NOEC: <3.25% <3.25%
I Alkalinity(mg/L CaCO3): 97 96 90 60-61 LOEC: 3.25% 3.25%
Hardness(mg/L CaCO3): 61 65 40 85-89 ChV: <3.25% <3.25%
Conductivity(µmhos/cm): 330 364 356 300-317 Method: Steel's Dunnett's
Total residual chlorine(mg/L): <0.10 <0.10 <0.10 - - i', M
Sample Temp.at Receipt(°C): 1.8 1.3 1 2 r' .' , Survival Growth
%Effluent Critical Calculated Critical Calculated
3.25% 10.000 10.000 2.410 7.892
S6.5% 10.000 10.000 2.410 7.500
13% 10.000 10.000 2.410 8.545
26% 10.000 10.000 2.410 6.590
Overall Analysis: 52% 10.000 10.000 2.410 4.927
Result: FAIL LOEC: 3.25% NOEC: <3.25% ChV: <3.25%
f DWR Report Form AT-5
1

Page 1 of 5
Environmental Testing Solutions.Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephales promelas
Client: Andrews WWTP County: Cherokee
NPDES#: NC 0020800 Outfall#: 001
Project#: t'1'LA.S
Dilution preparation:
Dilution prep(%) 3.25 6.5 13 26 52 Sample was not aerated or treated unless otherwise
Effluent volume(mL) 32.5 65 130 260 520 noted on this form. Sample was warmed to 25.0±1.0°C
Diluent volume(mL) 967.5 935 870 740 480 in a warm water bath and then diluted to the test
concentrations with moderately hard synthetic water '
Total volume(mL) 1000 1000 1000 1000 1000 (MHSW).
Test organism information: Test information:
Organism source: In-house culture Randomizing template: Grilt e_QlJ
Incubator number
Age: <24-hours old -A E
and shelf location:
Spawn date: Cili.1 . . Artemia CHM number: CHM1149
t0-0-b-1.1. ‘4AS •C-O Drying information for weight determination:
Hatch dates and times: 0.•4:14,--1.1.. OS®O Date/Time in oven: ;a.t;11. oaad•U.
*Initial oven temperature: 4o
Transfer vessel information: pH(S.U.)= -1.6& Date/Time out of oven: 10•tt•tt litS
Temperature(°C)= AA*l *Final oven temperature: C.•C, —
Average transfer volume(mL): <0.25 mL Total drying time: •1.'1-1+0J(.S
*60°C Oven,Thermometer SN:14-98585
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation,renewal, Sample MHSW
or termination number batch used
1 Time Analyst Time Analyst Time 1 Analyst used
o 10-04-22 oSo& e�013 - cn 13 Q N tt i of •01 o4 6"Z`t•ttA
1 loos22 OSW ItJO > 01"LO .L _
2 10-06-22 OSCX) 11 i130 8A` al CS It -1.1.io0S.1'S 0,t-1((5v2.-Lc
3 10-07-22 OS00 t OO of 10IA ,,id
4 10-08-22 0torj0
5 10-09-22 U�'rw! , i
6
01a00 t'LC30 0i10
�t
10-10-22 QCOt) ,�1 O`C)Oh. 1
7 10-11-22 Y
Ut."L',
Chemical analyses:
Parameter Reporting Limit Method number I Meter Serial number
pH 0.1 S.U. SM 4500-H+8-2011 I Accumet AR20 93312452
Dissolved Oxygen(D.O.) 1.0 mg/L SM 4500-0 G-2016 YSI Model 52CE 18D104324
Conductivity 14.9 µmhos/cm SM 2510 13-2011 Accumet AR20 93312452
Alkalinity 5.0 mg CaCO3/L _SM 2320 B-2011 Accumet AR20 93312452
Hardness 5.0 mg CaCOs/L SM 2340 C-2011 Not applicable Not applicable
Chlorine,Total Residual 0.1 mg/L ORION 97-70-1977 Accumet AB250 _ 92349123
Temperature 0.1°C SM 2550B-2010 Digital Thermometer `UfDtDa(® 5
Control information: Acceptance criteria Summary of test endpoints:
%Mortality: 01. _ 5 20% 7-day LCso(%) 'S-2.7
Average weight per initial larvae: O. U NOEC(%) 41,IS Z
Average weight per surviving larvae: 0.EEC, >_0.25mg/larvae LOEC(%) 3.1.87.
ChV(°%) <3.8.57.
IC2s(%) 4 3.LS 7.
SOP AT2O-Revision 6—Exhibit AT20.3

Page 2 of 5
Environmental Testing Solutions.Inc.
Species: Pimephales promelas
Client: Andrews WWTP Date: 10-04-22
Survival and Growth Data
Day CONTROL 3.25% 6.5%
ABCDEF G H I 1 K L
0
10 10 10 10 10 10 10 10 10 10 10 10
1 to 1'0 10 10 10 10 ID ID tp 10 I0 (D
2 (1) 10 10 ID 10 10 10 1D to 10 10 1p
I 3
I0 It) (0 (0 to to to to to I
4
1D ID (0 io 10 10 Io ro /0 10 t 0 10
5 aet. Bel `{el �cl � l 3rA
1 p 10 1 0 I D -1 Ci. —1 l0 10 5 -' 7
6 10 (0 10 10 ""1 gel d
Sze `sal � �,� `t _t
7
10 10 )0 10 '0 n `ck
*A=Pan weight(mg) l?.31 tag( )S.(s !l(.' loS I&73 13.q© ly.-$, is W Ob )3.01
Tray color code: NAVY
Analyst: g-C. (?j.4c6
Date: (A24ZZ
*B=Pan+Larvaeweight(mg) 2o•$3 23.06 25.0t 23.36 i7.7M 14.00 17.3'6 it-1.78.• tv.36, IS.`'►1 t8°.'4-i 14.77
Analyst: AT
Date: tt)1622
C=Larvae weight(mg)=B-A
— $•S'L 8.41 3.M S-7.- -L.55 0,JCt 1.s t 2.3'•i
Analyst:
Weight per initial number of larvae(mg)
=C/Initial number of larvae
Analyst: 31 S 1'
Y ®• `1• 0• 0- 0• O. 0- 0. 0. 0• 0- 0-
Average weight per initial Percent
number of larvae(mg) reduction cc �0"1 1, ®, C .21" 0.34 L bl.0 7.
from �' �p
control(%)
*Weight measurements performed using Cahn 28 Automatic Electrobalance,SN 41520.
Comment codes: c=clear, d=dead,fg=fungus, k=killed, m =missing,sk=sick,sm =unusually small,
Ig=unusually large, d&r=decanted and returned,w=wounded.
Comments: we TD
M.Q2Td�el.t�'°,3w3 �.�rL���J� C.OtJCQ.NT{Z►°rT10e�1S
?dacL-!o5e,)a C. e p.)FecTtcN.
SOP AT20-Revision 6—Exhibit AT20.3

Page 3 of 5
Environmental Testing Solutions.Inc.
Species: Pimephales promelas
Client: Andrews WWTP Date: 10-04-22
Survival and Growth Data
Day 13% 26% 52%
M N 0 P Q R S T U V W X
0
10 10 10 10 10 10 10 10 10 10 10 10
1 (O 10 10 10 1EJ 1b tO 1b ID 1O 1t 11)
2 10 tO (0 t0 ( 0 l0 10 (0 10 t0 tO to
3 ll� 10 ID 10 1p l0 t4 to t0 ( 0 tv ate
4 )O 10 10 10 10 10 10 /o to (O to
5 sik +� u.k. -LA, +4d1, +d +d +rA. ,,Jl. 3.1,
at at S- $ ID to al °1 G1 l a V
I
1', 7 6.141% -514 4, ,-kb -t,6k to g S
''�r9�l tL,f� el,�-xc ,�] G c��
{'A=Pan weight(mg) t5.q"D 6,/."tD t"l:'`J IL�Y@ 1 15.)U I / ► iS.pZ i 3. ' (,S. 3 1
Tray color code: KIP\JV 11-M
tAnalyst: 2 C
Date: 0q 2:22
l*B=Pan+Larvaeweight(mg) 1g.311 15.31. 1/.13 11.1'1 (q.2ct 1$. 70 17. 83 1q.lel 20.38 Zi.18 1q.15 j4.53
Analyst:
Date: )a1622
IC=Larvae weight(mg)=B-A
I S.Sb %,t'S -. -x..v4 4.la �s4 '.1.1.. 4.3o „,coo S ' s.st% -5. ko
Analyst:
Weight per initial number of larvae(mg)
C/Initial number of larvae
ti
Analyst: _ 0. t. ®.1 0. lD 0' t° ®- o. p 0. 0'
IA✓erage weight per initial Percent
1lirumber of larvae(mg) reduction ,t �g
from control f).1:10 (A.s�. 0•'1�1 53.io f. O.sst '40.1 i.
(%)
* eight measurements performed using Cahn 28 Automatic Electrobalance,SN 41520.
Comment codes: c=clear,d=dead,fg=fungus, k=killed, m =missing,sk=sick,sm=unusually small,
Ig=unusually large, d&r=decanted and returned,w=wounded.
Comments: ....
1
jSOP AT2O-Revision 6-Exhibit AT20.3
V

Environmental Testingbolutions,Inc
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1000.0)
Species: Pimephales promelas
Facility: Andrews WTP Entered by.
W
Kelley Keenan
Test start date: October 04,2022 Reviewed by:
Project number: 17295 li"'Sn Inc(
Sample numbers 221003.01,221005.33,221007.02
Concentration(%) Replicate I Initial number of Final number of larvae A=Pan weight(mg) R.Pan*Larvae larvae weight(mg) Weight/Surviving Mean weight/ Coefficient of variation Weight/Initial number Mean survival Mean weight/ Coefficient of variation Percent reduction from
larvae weight(mg) =B-A number of larvae(mg) Surviving number of Iteee..xsat eo•o•nin..e.c., of larvae(mg) I%) Initial number of I%l control(%)
I ((N) eel.....)1%) lyryae log)
A 10 10 12.31 20.83 8.52 0.852 0.852
B 10 10 14.59 23.06 8.47 0.847 0.847
_
Control C 10 10 15.69 25.01 9.32 0.932 0.886 4.8 0 932 100.0 0.886 4.8 Not applicable
O 10 10 14.25 23.36 9.11 0.911 _ 0.911
E 10 7 14.75 18.74 3.99 0.570 0.399
F 10 8 13.73 _ 19.00 5.27 0.659 0.527
3.25% G 10 5 14.83 17.38 2.55 0.510 0.655 24.8 0.255 52.5 0.317 59.6 64.2
H 10 1 13.90 14,78 0.88 0.880 0.088
10 6 14.78 18.36 3.58 0.597 0.358
1 10 4 13.08 15.42 2.34 0.585 0.234
6.54'o X 10 7 14.00 18.14 4.14 0.591 0.600 3.1 0.414
57.5 0.346 22.6 61.0
L 10 6 13.01 16.77 3.76 0.627 0.376
M 10 6 14.83 18.39 3.56 0.593 0.356
N 10 3 13.49 15.32 1.83 0.610 0.183
13% 0 10 6 13.90 17.13 3.23 0.538 0.572 6.1 0.323 47.5 0.270 30.5 69.5
P 10 4 14.95 17.14 2.19 0.548 0.219
a 10 7 _ 14.91 19.29 - 4.38 0.626 0.438
R 10 6 15.16 18.70 3.54 0.590 0.354
26% 5 10 6 _ 13.61 17.83 4.22 0.703 0.633 7.7 0 422 65.0 0.411 9.4 53.6
T 10 7 14.89 19.19 4.30 0.614 0.430
U 10 9 15.00 20.88 5.88 0.653 0.588
✓ 10 9 15.82 21.78 5.96 0.662 0.596
52% W 10 8 13.56 19.15 5.59 0.699 0.694 7.0 0.559 77.5 0.531 19.2 40.1
X 10 5 15.73 19.53 3.80 0.760 0.380
Outfall 001: MSD= Minimum Significant Difference
Dunnett's MSD value: 0.1735 PMSD= Percent Minimum Significant Difference
PMSD: 19.6 PMSD is a measure of test precision.The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10th percentile) =12%.
Upper PMSD bound determined by USEPA(90th percentile)=30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile,respectively,of PMSD data from EPA's WET Interlaboratory Variability Study(USEPA,2001a;USEPA,2001b).
USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-B-01-004 and EPA-821-8-01-005.US Environmental Protection Agency,Cincinnati,OH.

i
..Analyses KelleyEntered Keenanby:
Statistical Analyses Reviewed by:
Jim Sumner
1. Environmental Testing Solutions,Inc.
' Larval Fish Growth and Survival Test-7 Day Survival I
Start Date: 10/4/2022 Test ID: PpFRCR Sample ID: Andrews WWTP
Erd Date: 10/11/2022 Lab ID: ETS-Envir.Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas
Cc mments:
1 Conc-% 1 2 3 4
D-C o ntro l 1.0000 1.0000 1.0000 1.0000
3.25 0.7000 0.8000 0.5000 0.1000
6.5 0.6000 0.4000 0.7000 0.6000
13 0.6000 0.3000 0.6000 0.4000
26 0.7000 0.6000 0.6000 0.7000
52 0.9000 0.9000 0.8000 0.5000
1 Transform:Arcsin Square Root Rank 1-Tailed
Conc-% Mean N-Mean Mean Min Max CV% N Sum Critical
D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
*3.25 0.5250 0.5250 0.8014 0.3218 1.1071 43.216 4 10.00 10.00
*6.5 0.5750 0.5750 0.8620 0.6847 0.9912 14.867 4 10.00 10.00
*13 0.4750 0.4750 0.7591 0.5796 0.8861 20.120 4 10.00 10.00
*26 0.6500 0.6500 0.9386 0.8861 0.9912 6.464 4 10.00 10.00
*52 0.7750 0.7750 1.0977 0.7854 1.2490 19.920 4 10.00 10.00
I
Atixiliary Tests Statistic Critical Skew Kurt
Stiapiro-Wilk's Test indicates normal distribution(p>0.01) 0.91821 0.884 -1.078 2.25044
Equality of variance cannot be confirmed
Hypothesis Test(1-tail,0.05) NOEC LOEC ChV TU
S.eel's Many-One Rank Test <3.25 3.25
T'eatments vs D-Control
Dose-Response Plot
1
0.9 -
0.8 -
0.7 -
m
I a 0.63 _
1 cn0.5 -
85 0.4 -
0.3
0.2
' 0.1 -
1 0 .
I a, M co C•I
N
.cr r r
0
O
I
1

Entered by.
Kelley Keenan
Statistical Analyses Reletved by:
Jim Sumner
Environmental Testing Solutions.Inc.
Larval Fish Growth and Survival Test-7 Day Growth
Start Date: 10/4/2022 Test ID: PpFRCR Sample ID: Andrews WWTP
End Date: 10/11/2022 Lab ID: ETS-Envir.Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas
Comments:
Conc-% 1 2 3 4
D-Control 0.8520 0.8470 0.9320 0.9110
3.25 0.3990 0.5270 0.2550 0.0880
6.5 0.3580 0.2340 0.4140 0.3760
13 0.3560 0.1830 0.3230 0.2190
26 0.4380 0.3540 0.4220 0.4300
52 0.5880 0.5960 0.5590 0.3800
Transform:Untransformed 1-Tailed Isotonic
Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean
D-Control 0.8855 1.0000 0.8855 0.8470 0.9320 4.799 4 0.8855 1.0000
*3.25 0.3173 0.3583 0.3173 0.0880 0.5270 59.559 4 7.892 2.410 0.1735 0.3750 0.4234
*6.5 0.3455 0.3902 0.3455 0.2340 0.4140 22.551 4 7.500 2.410 0.1735 0.3750 0.4234
*13 0.2703 0.3052 0.2703 0.1830 0.3560 30.494 4 8.545 2.410 0.1735 0.3750 0.4234
•26 0.4110 0.4641 0.4110 0.3540 0.4380 9.381 4 6.590 2.410 0.1735 0.3750 0.4234
•52 0.5308 0.5994 0.5308 0.3800 0.5960 19.171 4 4.927 2.410 0.1735 0.3750 0.4234
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution(p>0.01) 0.95134 0.884 -0.4311 1.3907
Bartlett's Test indicates equal variances(p=0.11) 9.06531 15.0863
Hypothesis Test(1-tail,0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df
Dunnett's Test <3.25 3.25 0.17352 0.19596 0.20636 0.01037 9.2E-07 5,18
Treatments vs D-Control
Linear Interpolation(200 Resamples)
Point % SD 95%CL(Exp) Skew
1C05` 0.2818 0.0205 0.2434 0.3759 1.4498
IC10* 0.5637 0.0410 0.4869 0.7518 1.4498
IC15• 0.8455 0.0616 0.7303 1.1277 1.4498 1.0
IC20• 1.1274 0.0821 0.9738 1.5036 1.4498 0.9:
I C25* 1.4092 0.1026 1.2172 1.8795 1.4498
IC40` 2.2547 0.1641 1.9475 3.0073 1.4498 0.8-
1050• 2.8184 0.4052 2.4344 5.1367 3.6912 0.7-
•indicates IC estimate less than the lowest concentration •
w 0.6- ♦ O - .• •
0.5-
N •
K 0.4-
0.3-
0.2-
0.1-
0.0 0
0 20 40 60
Dose%
Dose-Response Plot
1
0.9
0.8
0.7
1-tail,0.05 level
r of significance
0.6
C9 0.5
0.4 •
0.3
0.2
01
0
O N w1 M N N
O
U
6

II
• I
Page 4 of 5
Environmental Testing Solutions.Inc.
Species: Pimephales promelas Date: 10-04-22
Client: Andrews WWTP
Daily Chemistry:
Temperatures performed at the time of test initiation,renewal or termination by the analyst identified in the Daily Renewal Information table
located on Page 1. Alkalinity,hardness and chlorine(total residual)performed by the analyst identified on the bench sheet specific for each
analysis and transcribed to this bench sheet.
Day
(Analyst identified for each day,performed pH,D.O.and conductivity measurements only.)
0 I 1 I 2
Analyst vic cc IA' VL' "C 1/L,
Concentration Parameter
pH(S.U.) ,,.Si (9•((9 (8 0. d .510 (9.q�
..-
—lc
Dissolved oxygen(mg/L) '4,{� ). �� '48 r4.-1
CONTROL, Conductivity(µmhos/cm) 300 slC 30 -
MHSW Alkalinity(mg CaCO3/L) (s D ....-t.. LA)
Hardness(mg CaCO3/L) R S iQ &is
Temperature(°C) -%.4. f L '1.4`A ^‘90-1 Z`-l< 3 'L°{.& -t (0pH(S.U.) 13O :.OP ;al i 1 ,,s
Dissolved oxygen(mg/L) /4.s IIEB- i_--
3.25%
Conductivity(µmhos/cm) 30/ 3 Os
Temperature(°C) Z‘11 �� '�,�•.1 'ta,., -Li�/�i` -�4,
/4
pH(S.U.) -.30 --o 72C i J•+ 6V (0 c ,0
Dissolved oxygen(mg/L) 7,S a 8
6.5% 3
Conductivity(µ SQmhos/cm) 50C) ' pC
(3 _.
Temperature(°C) 1•14•1 .A.q.", nu.i, 1�•L INI '1.4.k-k
pH(S.U.) .atS q"a J . .2® b./ cc.c13
Dissolved oxygen(mg/L) / ,(A WI 8 t/.0 /r,/�
13% 'e��
Conductivity(µmhos/cm) 300 jOg 31 il
Temperature 1°C) 114l•l �p -NA'S 1.� y•1 —L '5 ',M.& —Lg.Ll I
pH(S.U.) 2 T 5 � 1. 1 l Ct L . C.
Dissolved oxygen(mg/L) --.) /7r4 0 (� .
26% r.
Conductivity(µmhos/cm) '3(y 3(7- 1�
Temperature(°C) 'l.°{., ► T Lt.6 -9 '1„°+,g '�1.�.
^ 3
pH(S.U.) 7.2.1 . Vci i r it.ZJ 3 -.. 'MERINO
Dissolved oxygen(mg/L) �-' .� ��.
52% �T p _
Conductivity(µmhos/cm) O d�� .3 Z'VA.
L �•3G"
Temperature(°C) •�ki• .14. ' A. 6' 1••t-..•, '�.Z' 0 �.�1 .SpH(S.U.) l� - 1.00
`A
Dissolved oxygen(mg/L) „(, ,64
Conductivity(µmhos/cm) 370 I 31n,--/
100% Alkalinity(mg CaCOiL) t 1 i
Hardness(mg CaCO3/L) (p Pt 6 S
Chlorine(mg/L) C 0.10 G 0.10
Temperature(°C) 211.4:, is-o
Initial Final Initial Final Initial Final
I
SOP AT2O-Revision 6—Exhibit AT20.3
I

I-4' -1 Page 5 of 5
Environmental Testing Solutions,Inc.
I
Species: Pimephales promelas
Client: And rews WWTP Date: 10-04-22
Day
(Analyst identified for each day,performed pH,D.O.and conductivity measurements only.)
3 4 5 6
Analyst fr(_, (r< ifk-. (1(,, 1k
Concentration Parameter I pH U.) j3 u�; /�.0 /i'•� ./t (( e i /i.
Dissolved oxygen(mg/L
)
� .� 10, "�:' �3 --4-:)-'
v_J
1
CONTROL, Conductivity(µmhos/cm) 3u4 3 3vt-. 3 CI'
1 MHSW Alkalinity(mg CaCOs/L) �,t'L k`ep 7
Hardness(mg CaCOm/L) `, r &.1 f�1 `- G P - i.._ t . )
7{( Temperature(°C) Zppl- b 1,e..yS-t 1-."rd- ! -/�p./�/t6 NA.6 r�A.A.1 'NA•1 1.A.1D
IpH(S.U.) _7-OO ti..'I3 ,I"c' -/ (9gl 14•.^ 4..Ob /�7ce l.041
Dissolved oxygen(mg/L) 11 1-.3 5 01k-( _ '. 1 '7 r i 'j-;[ (a. -
3.25% Conductivity(µmhos/cm) 3IU ,3Cto. '3IL 313
Temperature(°C) .-t-t.`6•1 �i1-'t-6 -t�1.'4 1 `,..k. 1 /1_4• 1 1...`l-( /L-t. k -LA-4
pH(S.U.) 4,1( l4.412i d.1✓ I4I--•(/T . 1f ).••O2. 4, -1. 14.WI
Dissolved oxygen(mg/L) ,i -1-.a b [r�•} (Q y,t„u �r r •1 t✓.
6.5% Conductivity(µmhos/cm) 3l I t p 1'4 y e, ✓1.C3 `` �g
I Temperature(°C) 124.. 1 '!a�A 1..`it.'1 r-A.t /�IA. ) ,•'l-tt ^-..'l. g- �n•'1 A
pH(S.U.) -4-.11 le-t4 4'I`O y.aoR 7 L12- q.0Z "�i.'J(O t,.•l�
Dissolved oxygen(mg/L) 40,o 1-,� 'Y IO 6 ' 1.,t ')-•9 La,to
13% — 2
Conductivity(µmhos/cm) 3tR ^� GG [3tI y� — c C.0 , -1 ,_. 318 L -. a
Temperature(°C) ' `- 1 1.�.-9 -A.O �.�• 6 LA-c a•k.L Z.A•6 `A-
pH(S.U.) --11 _ L•Q3 a-:ro3 /4•0")- 1-.L1 D `�..0'4 '�.3(0 ,1- l 0
-Y Dissolved oxygen(mg/L) 010 4,3 7 a',''f' -S "�,O 6,0 (a} Z
26°� 32y 3oct 331 3 2c L
-1
Conductivity(µmhos/cm)
Temperature �.''i- 1 �.�-to AA. -1.A.k L4.et I A. k l.�•I -l..`A-•�
pH(S.U) 'q.03 (O.'IA (t-.190 'fir-0(p '�-.40 ' -.0(0 ��'.3l '1..,{03-
�. Dissolved ox52% ygen(mg/L) CJ�` (p,$ -y 5 `"-� /TAO �7 Vu '-i ^
Conductivity(µmhos/cm) 3,+ 1 330 I 34• ,1 3'fJ { -a a�
Temperature(°C) _14• �1.t-q •Lt4• F IA- LA-at mL4.-L. 1.-1-9 {' .1
1 pH(S.U.) tQ p.J
Dissolved oxygen(mg/L) ice 5
Conductivity(µmhos/cm) 35
100% Alkalinity(mg CaCO,/L) q Q
Hardness(mg CaCOa/L) y 0 - �! I
I Chlorine(rng/L) 0 a I eJ i __..�.
Temperature(°C) ZY.
Ci
Initial Final Initial Final Initial Final Initial Final
SOP AT2O-Revision 6—Exhibit AT20.3

xr'. ...:gym r
- 351 Depot Street
° = Asheville,NC 28801
r - Phone: [phone]
( Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Earth Environmental Services NPDES#: NC0020800 Pipe#: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Pimephales promelas Effluent dilution: 3.25,6.5, 13,26,52%
Test type: Chronic Definitive Parameter code: THP6C
Z.
Sample information: (to be completed by sample collector)
g
Comp isite sample: Sample location: f47 1��'-�l 1'
I Stall K ate: /0/.Z42- Time: (;;3()4,11 Volume collected for testing: / 6 4//6 n
e End d ate: jo/3129.— Time: (;f 3c1,q,lu Number of containers filled for testing: i ,.
Number of samples per hour: 4(�a.,,,, I � Method of transport to laboratory: corn rt.e f
Chil e i during collection? ( -T`e Yei Comments: x
If chit ed,specify temperature: 3 c C 1
AT¢tple rinse sample container with sample before filling. Completely fill the sample container with no air space. 5
t{j
$ Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. a
Sample elk ody: (to be completed by sample collector and facility personnel)
Sample 4oBected by:
J' r,/ C�3C?61,r
P i Signature Data and time •3,
Relinquished by: Received by: i
/J/ari�''.6e!Hey 7�' 764,,(.44 -oo: �, 1
l Print ! Signature Date and tune Prim Signature
����JJJJ tgnatur Date and time
Relinqui It:d by:
Received by:
10032t_
Pant Signature Dat,,and time Print Signature Date and tune i
,w.,r>wa.-,_ a.�..,.._,.s .u3�. ,o..r. ....� ++..,�..... .-,c.�r�...n. .�x_.,-a .... n�«.�..x. �.�r�u
Sample recept information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
►o(e)3/z id�u3a.�
f-ct.....s.C-311bLA—• -3SAn1 U� Oes 35-
1 Print Signature Date and time Print Signature Date aFia time
I
Cus oily seals intact?: - - = Sample temperature upon receipt at ETS(°C):
Yes No Not used
Sam tks received in good condition?: 121 -
Yes No Total residual chlorine upon receipt at ETS: _ J
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) Present Abs..mt
Tratlk ng number: 0 fs
Project nuntber:n -2AS Sample numbeMI00.3.0I
Comm: a:

gi
351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Earth Environmental Services NPDES#: NC0020800 Pipe#: 001 County: Cherokee
'1,4.ndrews WWTP Purchase order:
I
Species: Pimephales promelas Effluent dilution: 3.25,6.5, 13,26,52%
Test type: chronic Definitive Parameter code: THP6C
Sample information: (to be completed by sample collector)
1 1
Compol<ite sample: Sample location: '- a2.4 f
Stan'date: 'tp .,2 Time: Volume collected for G,3o�rrl testing: /6 l/ofri
End date: t I, Time: (,)3'f 4.M Number of containers filled for testing:
if tst
Number of samples per hour: )X /� Method of laboratory:to transport /_�
p n'� L�/'JL'r
Chil ei during collection? yes Comments: li
If coil ed,specify temperature:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. t
a
Sample cuS:ody: (to be completed by sample collector and facility personnel)
Sample dollected by:
c taLL:4_ (a/�2Z S.
PPS lureaill
r.
A" S Date and time
Relinqui;lt_d by:
Received by:
sz- ��h4�' , r? I io/S/zz
i rk -l�.r --i i /S/zC, 1:W4iAw 7:yyA,�
Print \ Signature Date and time Pint Signature Date and time
Relinqui,6pd by: Received by:
L :L,L ;nmnT
Signature Date and
1
Sample rec®pt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by: /t
I .0,S•22.Ti,' (0/er/2L /0
F3 01~wC.Dro..s `{0 kf�`' gitn logo
Print Signature Dace and time Print Signature Date and time
Custody seals intact?: - MN © Sample temperature upon receipt at ETS(°C):Yes No Not used 1.3 a
Samp es received in good condition?: 1."--1 1-7
Yes No Total residual chlorine upon receipt at ETS: _ J
�( (DPD Presence/Absense Indicator,MDL=0.10 mg/L) Pr
e`ent Absent
Tra g number: Project number:��Z`1QS
Sample number: 00.100 3
Comm ts:
1

•
.;., n +.ti. aa*.�r,,.z ars•,,g-,,.
351 Depot Street
I Asheville,NC 28801
Phone: [phone]
Fax: [phone]
I
EnvironmentalTesting Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Earth Environmental Services NPDES#: NC0020800 Pipe#: 001 County: Cherokee
Andrews WWTP Purchase order:
t
Species: Pimephales promelas Effluent dilution: 3.25,6.5, 13,26,52%
Test type: Chronic Definitive Parameter code: THP6C
.>.4.rr.. ,,. .. .�-�,,..._ _ � -.•,:__ . _�.,� .�._._::,. �-tea
Sample i formation. (to be completed by sample collector)
Comp site sample: Sample location: 7t,,4;11
R
Sta ate: !.? a Time: 4230,+AI Volume collected for testing: a2, e,citoe?S i
End ate: 22— Time: 6,57,Q-/t4 Number of containers filled for testing: a
Nu b r of samples per hour: ig-pra 4. (4 Method of transport to laboratory: Calf i e✓' a
Chil ed during collection? 'r 5 Comments: w
If chilled,specify temperature: ,2°e
Tr ple rinse sample container with sample before filling. Completely fill the sample container with no air space.
i
' Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample et 5 ody: (to be completed by sample collector and facility personnel)
i
Sample tq/ected by: 1
Marrr �}� /oh/-Z
AP t i Hint ��` �. (0 5i A v)
t Signature Date and time
Relinquiahnd by: 5 Received by:
Mari(VC': /o/7//2- COCi.Ade( lam, t� / /0/7 2z
P t Signature ate and time P run SIgnature Date and time •
Relinqui,bed by: Received by:
ek 0 t)-37Z—
Print Signature Date and time
Print Signature ;Pate and time
1
Sample re 'Opt information: (to be completed by ETS personnel)
Relinqui lice'to ETS by: Received at ETS by:
k, , ,,,,,e- _ 0q51) IXEL- V--- Ogg)
/0-17.12- /0.(l)•2L
Pam Signature Date and tint, Prim Signature Date and time
Cus oily seals intact?: — — Sample temperature upon receipt at ETS(°C): j eZ
Yes No\ Not used
Sam rles received in good condition?: IIIE -
Yea No Total residual chlorine upon receipt at ETS: _ IMII
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) Pr"ent Abu"t
Tra.lung number: tP �
Project number:112•4c Sample number: ,02.-
Commto a:
i

Pimephales promelas
. . Chronic Reference Toxicant Control Chart
„„ Environmental Testing Solutions,Inc. Source: In-house Culture
0.9 1 I I I I I I I I I I I I I 1 I I I I I
- Control Limits (± 2 Standard Deviations) -
0.8 - -
•
0.7 - • • •
_n . • _
.., • •
- • • • • •
U • -
•
-
to
I I I I I 1 I I I I I I 1 I I 1 I I I I
iri
t" 1.2 1 I I I I I I I I I I I I I I I 1 I I I
V
oWarning Limits -
-
• • �...
•
• • • • i • •
•
0.4
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
ON- 60%OQ.0°0$0�13 0a,03 OS' 1°05 11�1 11 0� 01�a 01��0%��pa��' Og�3 O6��01 1�01 1ti 0%�2 OS 10.0� L
Test date
• i 7-day IC25=25%inhibition concentration. An estimation of the potassium chloride concentration
which would cause a 25%reduction in Pimephales growth (calculated using ToxCalc).
---- Central Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
— -- Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
..-. .= Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
.. USEPA Warning Limits (mean logarithmic IC25±SA 75 converted to anti-logarithmic values,
SA 75=75th percentile of CVs reported nationally by USEPA)
inme sa Ind
ne:,b..ea ey
m Sumner
e at

‘\...._______„
, , .10
.,
. . . f..,(4,A,
PO Box 7565
Asheville, NC 28802
— Phone: [phone]
,. Environmental Testing Solution;Inc.
Fax: [phone]
-cember 19, 2022
s. Wanda Ladd
:r:rth Environmental Services, Inc.
Bison Lane
urphy, NC 28906
Project Number: 17454
»t Start Date: December 06, 2022
cility Tested: Andrews WWTP
nclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Parameter
Code Test Procedure, Method Number Result (ChV)
THP6C Fathead minnow, Pimephales promelas,Larval Survival and Growth Chronic
EPA-821-R-02-013, Method 1000.0 >$2%
III toxicity testing results required as part of your permit must be entered on the Effluent Discharge
1 onitoring Form (MR-1) for the month in which it was performed, using the parameter code THP6C.
Additionally, the DMR Reporting Form AT-5 must be signed and e-mailed or mailed to the following
ac dress:
ATForms.ATBCa�ncdenr.clov
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh, NC 27699-1621
Please call if you have any questions concerning these results.
Sin:erely,
/(floVv...--___
Jim Sumner
Laioratory Supervisor
1
North Carolina Certificate Numbers: Biological Analyses: 037,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001

t
-�� PO Box 7565
Asheville,NC 28802
Phone: [phone]
• - Fax: [phone]
Environmental Testing Solutions,Inc.
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date: December 19,2022
Facility: Earth Environmental Services,Inc. NPDES#: NC 0020800 Pipe#: 001 County: Cherokee
Andrews WWTP
Laboratory Performing Test: Environmental Testing Soluti.ns,Inc.,Certificate#037 Comments:
Signature of Operator in Responsible Charge(ORC): ,, : /%' L,. ,
ORC Phone/E-mail: f ;£f 7 76gd e/ t. k1 /oni*andr?Ji r,Or9 Project#: 17454
Signature of Laboratory Supervisor: LA.0.....-s---•--- Sample#: 221205.02,221207.20,221209.08
e-Mail to: ATForms.ATBPncdenr.gov Test Organisms: Pimephales promelas
Or Mail Original to: North Carolina Division of Water Resources Supplier
Water Sciences Section/Aquatic Toxicology Branch In-house Culture
1621 Mail Service Center Begin hatch: 12-05-22 1455
Raleigh,NC 27699-1621 End hatch: 12-06-22 0500
Test Start Date: December 06,2022
Replicate number
1 2 3 4
Control Surviving number of larvae 10 10 10 10 Survival(%) 100.0
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.799
Weight/original(mg/larvae) 0.681 0.772 0.765 0.976 Average wt/ 0.799
surviving(mg)
%Effluent Surviving number of larvae 10 10 10 10
3.25% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.693 0.695 0.765 0.737 Average wt(mg) 0.723
%Effluent Surviving number of larvae 10 10 10 10
6.5% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.795 0.666 0.805 0.620 Average wt(mg) 0.722
%Effluent Surviving number of larvae 10 10 10 10
13% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.727 0.683 0.863 0.719 Average wt(mg) 0.748
%Effluent Surviving number of larvae 10 9 10 10
26% Original number of larvae 10 10 10 10 Survival(%) 97.5
Weight/original(mg/larvae) 0.709 0.643 0.727 0.813 Average wt(mg) 0.723
%Effluent Surviving number of larvae 9 10 10 10
52% Original number of larvae 10 10 10 10 Survival(%) 97.5
Weight/original(mg/larvae) 0.730 0.636 0.679 0.649 Average wt(mg) 0.674
Water Quality Data
Day 0 Day l Day 2 Day 3 Day 4 Day 5 Day 6
Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final
Control
pH(SU): 7.29 7.23 7.27 7.16 7.44 7.24 7.26 7.16 7.12 7.22 7.15 7.17 7.26 7.11
DO(mg/L): 7.8 7.8 7.7 7.3 7.7 7.2 7.8 7.9 7.8 7.4 7.7 7.5 7.8 7.5
Temp.('C): 24.8 24.8 24.8 24.7 24.8 24.7 24.7 24.7 24.8 24.5 24.8 24.7 24.8 24.6
High Concentration
pH(SU): - 7,29 7.19 7.31 7.04 7.19 7.11 7.22 7.12 7.21 7.18 7.29 7.13 7.27 7.07
DO(mg/L): 7.7 7.9 7.9 7.4 7.9 7.2 8.0 7.5 8.1 7.2 7.8 7.3 8.0 7.2
Temp.(°C): 25.0 24.7 25.0 24.7 25.0 24.5 24.8 24.8 24.9 24.6 24.9 24.5 24.8 24.5
Sample Information Sample 1 Sample 2 Sample 3 Control Analyses Survival Growth
Collection start date: 12-04-22 12-06-22 12-08-22 ,.r, - ..,°,„ Normal: Yes Yes
Grab: • . T»�.,. .,,. Hom.Var. Yes Yes
Composite duration: 24-h 22.25-h 22.5-h ,, NOEC 52% 52%
Alkalinity(mg/L CaCO3): 63 59 65 62-63 LOEC: >52% >52%
Hardness(mg/L CaCO3): 61 67 80 84-88 ChV: >52% >52%
Conductivity(µmhos/cm): 258 186 209 300-311 Method: Visual lnsp. Dunnett's
Total residual chlorine(mg/L): <0.10 <0.10 <0.101; ,;„.
Sample Temp.at Receipt('C): 0.6 2.1 0.3 Survival Growth
%Effluent Critical Calculated Critical Calculated
3.25% . . 2.410 1.343
6.5% . 2.410 1.361
13% . 2.410 0.893
26% . 2.410 1.335
Overall Analysis: 52% . 2.410 2.210
Result: PASS LOEC: >52% NOEC: 52% ChV: >52%
DWR Report Form AT-5
I

•
R WW ' Page 1 of 5
•
Environmental Testing Solutions,Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephales promelas
Client: Andrews WWTP County: Cherokee
NPDES#: NC 0020800 Outfall#: 001
Project#: t"14 A
Dilution preparation:
Dilution prep(%) 3.25 6.5 13 26 52 Sample was not aerated or treated unless otherwise
Effluent volume(mL) 32.5 65 130 260 520 noted on this form. Sample was warmed to 25.0±1.0°C
Diluent volume(mL) 967.5 935 870 740 480 in a warm water bath and then diluted to the test
concentrations with moderately hard synthetic water
Total volume(mL) 1000 1000 1000 1000 1000 (MHSW).
Test organism information: Test information:
Organism source: In-house culture Randomizing template: 6L49-
Incubator number
Age: <24-hours old and shelf location: 1 c°
Spawn date: 1V°10•1.•L Artemia CHM number: CHM1149
iti•OS• rt. tAS5 Drying information for weight determination:
Hatch dates and times: 'gib kA,-0b•t"L_ OSOO Date/Time in oven: 11-rb•VL P1+x7
_ *Initial oven temperature: t#p•C
Transfer vessel information: pH(S.U.)= —WAFtDate/Time out of oven: l'1•.A•11.. n-50
Temperature(°C)= 1.5•1— *Final oven temperature: (®4•L
Average transfer volume(mL): <0.25mL Total drying time: '4l•1$toAcl,
*60°C Oven,Thermometer SN:14-98565
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation,renewal, I Sample MHSW
or termination number batch used
Time Analyst Time Analyst Time Analyst used
0 12-06-22 0S05 ,,( V1.30 Ul2S A. 1Z%'WS.01.. 13o•z1 S
1 12-07-22 OSoe n MD G11© At I. y
2 12-08-22 0 otl 1N 06 �! r.'n t 0 h -,-Lalfl. \\-3E tL
3 12-09-22
r u` f. �!+
C)S� yit t ODO e t°
4 12-10-22 (ADO (t \ v o0 V. 0 etn— k( I.A.e1011a.Olt VtOka•An-
5 12-11-22
ono® k ,Zoo 4r nVO
\•,,
6 12-12-22 USSS 11.00
�. A oleos A
7 12-13-22 O Ir9 L
YIL
Chemical analyses:
Parameter Reporting Limit _ Method number Meter Serial number
pH 0.1 S.U. SM 4500-H+B-2011_ Accumet AR20 _ 93312452 _
Dissolved Oxygen(D.0.) 1.0 mg/L SM 4500-0 G-2016 YSI Model 52CE 18D104324
Conductivity 14.9 µmhos/cm SM 2510 B-2011 Accumet AR20 93312452
Alkalinity 5.0 mg CaCO3/L SM 2320 B-2011 Accumet AR20 93312452
Hardness 5.0 mg CaCO3/L SM 2340 C-2011 Not applicable Not applicable _
Chlorine,Total Residual 0.1 mg/L ORION 97-70-1977 Accumet AB250 92349123
Temperature 0.1°C SM 2550E-2010 Digital Thermometer 1Si9Lbgb`S
Control information: Acceptance criteria Summary of test endpoints:
%Mortality: 01. _ S 20% 7-day LCso(%) >$L 7.
Average weight per initial larvae: 0.11E NOEC(%) _ S Z in
Average weight per surviving larvae: 0.."-Pt CI >_0.25mg/larvae LOEC(%) SZ 1.
ChV(%) — 7SZ 1.
IC25(%) )SZ. 7.
SOP AT2O-Revision 6--Exhibit AT20.3
I

Page 2 of 5
Environmental Testing Solutions,Inc.
Species: Pimephales promelas
Client: Andrews WWTP Date: 12-06-22
Survival and Growth Data
Day CONTROL 3.25% 6.5%
ABCDEFGHI J K L
0 10 10 10 10 10 10 10 10 10 10 10 10
1 10 10 10 10 to t0 /D ID to to /0 10
2 tb /0 11) 10 ID /0 tO t0 /0 ID It) t0
3 10 10 10 t0 ID 10 10 10 1 ® 10 10 tO
4 to tD (0 10 10 tO tb 10 tti 10 10 to
5 10 10 10 1
0 10 ID ID 10 r0 to 10 to
6
10 to t0 1t3 � !p t0 t D ID it) 10 1D
10 ( 0 tp 1 ) 10 ID t0 1D t 0 1
0 t® 16
*A=Pan weight( ) 3.2q t� 1�IS 1;.Cj 1 )2.(9S 11.4E !S• )2.52 1313 �L t. 15.12 1G1.1.
Tray color code: ytaC i
Analyst:
Date: 1l-Z6i-ZZ
*B=Pan+Larvae weight(mg) Z01L 23. O 222.E 23.07 I!5%1V- 3.30 igv7l 21.16 2 LZ 23.13 ZOA.
Analyst:Z
Date: 12- tS_2Z
C=Larvae weight(mg)=B-A
(*At 1.11. .VS d1.16 6s.4'S1 t9.45 1.65 1.31 1.4S b.1.6. 9. iS 4,10
Analyst:
Weight per initial number of larvae(mg)
=C/Initial number of larvae
n/ 0
% A. ^ems 6, �� �� �� ^"5^ Cx v� �e®� xi)
Analyst: _ p° ®�
O' O' 0. ®* 10' ®` O' cr 0' 0*
Average weight per initial Percent
number of larvae(mg) reduction
from Q.1aldl 0.115 g.S 7. 0.111. °Lk- 7,
control(%)
*Weight measurements performed using Cahn 28 Automatic Electrobalance,SN 41520.
Comment codes: c=clear,d=dead,fg=fungus, k=killed, rn =missing,sk=sick,sm=unusually small,
Ig=unusually large, d&r=decanted and returned,w=wounded.
Comments:
SOP AT2O-Revision 6—Exhibit AT20.3

Page 3 of 5
Environmental Testing Solutions,Inc.
Species: Pimephales promelas
Client: Andrews WWTP Date: 12-06-22
Survival and Growth Data
I Day 13% 26% 52%
M N 0 P Q R 5 T U V W X
0
10 10 10 10 10 10 10 10 10 10 10 10
1 I 10 It 16 10 10 10 10 / 0 / 0 10 /0
2 10 1 (0 to L0 to 10 l0 10 10 10 10 10
I _
3 10 in !0 in. I ® 10 10 /0 10 IO 10 / 0
4 I® 10 10 /O 10 44 10 10 IO 10 10 0
5 It l0 ID ID /O at 10 /0 10 10 / 0 /0
6 i0 lb ID It 10 at 10 /0 c`d /0 /0 10
10 ( p tp 10 10 ci 10 10 Q 10 /p /0
A=Pan weight(m ) 15.90 1q (.01.& 1t►.�l 1�.%- 141.7g 12.43 /14.22 Py.(O% 1�y 04.0 I 12.3S Iy,i 19
ray color code: v€.i3 Vt
nalyst: �(-
ate: (t-Zq-2Z
122�{t B=Pan+Larvae weight(mg)
21.L4q �Z3.2Z 21.5e- 21.�1.d 1g.3(0 21.1 c1 22.(81 2Z 1L4 2074 101.1g 20.06
1+nalyst: Gf'c
G ate: i Z-15-ZZ
i =Larvae weight(mg)=B—A
1.1•1 c..g3 $.1s3 1.1C '.e°1 60•°$N 1.2.1 g.t3 A.10 L..bto t.,i9 ("'‘VI
jnalyst:
1Ti
eight per initial number of larvae(mg)
C/Initial number of larvae
a�
nalyst: �. �. b• d. ®' ®. ® �, ®, 0 �-
1S . o.
erage weight per initial Percent Q
mber of larvae(mg) reduction 0.-i & �. 1. ,'1'1 q. 7 ,1
from control ®. -i "1 7
(%)
*' eight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520.
Comment codes: c=clear, d=dead,fg=fungus, k=killed, m =missing,sk=sick,sm =unusually small,
Ig=unusually large, d&r=decanted and returned,w=wounded.
Comments:
SOP AT20-Revision 6—Exhibit AT20.3

1 .
Environment/Wresting Solutions,int.
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1000.0)
Species: Pimephales promelas
Facility: Andrews WWTP
Entered by.
Kelley Keenan
Test start date: December 06,2022 Reviewed by:
Project number: 17454 Jim sun Ber
Sample numbers 221205.02,221207.20,221209.08
Concentration(%) Replicate Initial number of Final number of larvae A=Pan weight(mg) B=Pan a Larvae Larvae weight(mg) Weight/Surviving Mean weight/ Coefficient of variation Weight/Initial number Mean survival Mean weight/ Coefficient of variation Percent reduction from
larvae weight(mg) =B.A number of larvae(mg) Surviving number of Ira••..wurar w,.,,,....„.no, of larvae(mg) 1%1 Initial number of I%) control(%)
larvae(mg) or inra•I I%) larvae(mg)
A 10 10 13.29 20.10 6.81 0.681 0.681
Control B 10 10 15.98 23.70 7.72_ 0.772 0,772
C 10 10 14.58 22.23 7.65 0.765 0.799 15.7 100.0 0.799 15.7 Not applicable
0.765
D 10 10 13.91 23.67 9.76 0.976 0.976
E 10 10 12.65 19.58 6.93 0.693 - 0.693
3.25% - F 10 10 11.45 18.40 6.95 0.695 0.695
G 10 10 15.73 23.38 7.65 0.765 0.723 4.8 0.765 100.0 0.723 4.8 9.5
H 10 10 12.52 19.89 7.37 0.737 0.737
10 10 13.23 21.18 7.95 0.795 0.795
I
6.5% 1 10 10 14.61 21.27 6.66 0.666 0.666
0.722 12.8 100.0 0.722 12.8 9.6
10 10 15.12 23.17 8.05 0.805 0.805
K
L 10 10 14.78 20.98 6.20 0.620 0.620
M 10 10 15.70 22.97 7.27 0.727 0.727
13% N 10 10 14.66 21.49 6.83 0.683 0.693
O 10 10 14.59 23.22 8.63 0.863 0.748 10.6 0.863 100.0 0.748 10.6 6.3
P 10 10 14.35 21.54 7.19 0.719 0.719
Q 10 10 14.75 21.84 7.09 0.709 0.709
26% R 10 9 12.93 19.36 6.43 0.714 - 0.643
0.741 6.697.5 0.723 9.7 9.5
5 10 10 14.22 21.49 7.27 0.727 0.727
T 10 10 14.68 22.81 8.13 0.813 0.813
U 10 9 14.84 22.14 7.30 0.811 0.730
52% V 10 10 14.41 20.77 6.36 0.636 0.636
0.694 11.6 97.5 0.674 6.2 15.7
W 10 10 12.38 19.17 6.79 0.679 0.679
X 10 10 14.16 20.65 6.49 0.649 0.649
Outfall 001: MOD= Minimum Significant Difference
Dunnett's MSD value: 0.1363 PMSD= Percent Minimum Significant Difference
PMSD: 17.1 PMSD is a measure of test precision.The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10th percentile)=12%.
Upper PMSD bound determined by USEPA(90th percentile)=30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile,respectively,of PMSD data from EPA's WET Interlaboratory Variability Study(USEPA,2001a;USEPA,2001b).
USEPA. 2001a,2001b.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-1301-004 and EPA-821-8-01-005.US Environmental Protection Agency,Cincinnati,OH.

1
a
Emmen by:
Kelley Keenan
Statistical Analyses Revioned by:
lim Sumner
Environment(Testing Solution.Inc
Larval Fish Growth and Survival Test-7 Day Growth
Start Date: 12/6/2022 Test ID: PpFRCR Sample ID: Andrews
End Date: 12/13/2022 Lab ID: ETS-Envir.Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas
Comments:
Conc-% 1 2 3 4
D-Control 0.6810 0.7720 0.7650 0.9760
3.25 0.6930 0.6950 0.7650 0.7370
6.5 0.7950 0.6660 0.8050 0.6200
13 0.7270 0.6830 0.8630 0.7190
26 0.7090 0.6430 0.7270 0.8130
52 0.7300 0.6360 0.6790 0.6490
Transform:Untransformed 1-Tailed Isotonic
Conc% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean
D-Control 0.7985 1.0000 0.7985 0.6810 0.9760 15.698 4 0.7985 1.0000
3.25 0.7225 0.9048 0.7225 0.6930 0.7650 4.823 4 1.343 2.410 0.1363 0.7307 0.9150
6.5 0.7215 0.9036 0.7215 0.6200 0.8050 12.843 4 1.361 2.410 0.1363 0.7307 0.9150
13 0.7480 0.9368 0.7480 0.6830 0.8630 10.564 4 0.893 2.410 0.1363 0.7307 0.9150
26 0-7230 0.9054 0.7230 0.6430 0.8130 9.686 4 1.335 2.410 0.1363 0.7230 0.9054
52 0.6735 0.8435 0.6735 0.6360 0.7300 6.199 4 2.210 2.410 0.1363 0.6735 0.8435
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution(p>0.01) 0.95114 0.884 0.70237 0.38126
Bartlett's Test indicates equal variances(p=0.36) 5.47691 15.0863
Hypothesis Test(1-tail,0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df
Dunnett's Test 52 >52 1.92308 0.13634 0.17074 0.0067 0.0064 0.42089 5,18
Treatments vs D-Control
Linear Interpolation(200 Resamples)
Point % SD 95%CL(Exp) Skew
IC05` 1.913
IC10 28.285
IC15 49.256 1.0
IC20 >52
IC25 >52 0.9
IC40 >52 0.8-
IC50 >52 0.7-
indicates IC estimate less than the lowest concentration
d 0.6-
a
c
a0.5-
a
&0.4-
i 0.3
II 0.2-
0.1 Z® -4
0.0 rT-
0 20 40 60
Dose%
Dose-Response Plot
1.2
1
0.8
c _ _ 1-tail,0.05 level
c9 0.6 of significance
n7
is 0.4
0.2
0
o n r
C N �
O
6

. . I
Page 4 of 5
Environ mental Testing Solutions,Inc.
Species: Pimephales promelas Date: 12-06-22
Client: Andrews WWTP
Daily Chemistry:
Temperatures performed at the time of test initiation,renewal or termination by the analyst identified in the Daily Renewal Information table
located on Page 1. Alkalinity,hardness and chlorine(total residual)performed by the analyst identified on the bench sheet specific for each
analysis and transcribed to this bench sheet.
Day
(Analyst identified for each day,performed pH,D.O.and conductivity measurements only.)
0 1 2
Analyst c £-G h VC ./1-' N`' rG�
Concentration Parameter l` !
pH(S.U.) '4.2ct 3.73 7.2 /}.t(p /4.1.1(-1 -4.2
Dissolved oxygen(mg/L) 't.I 1.6 --)--1 •3 -4.. . ..,.._
CONTROL, Conductivity(µmhos/cm) 30(.4 t( 3 00
MHSW Alkalinity(mg CaCO3/14 tp^L .11,, ko`5
Hardness(mg CaCO3/L) as-k • . a a
Temperature(°C) '1'`' .. g •1..`.g- -LA. 6 --L�`{..t 'NA.S AA.,
pH(S.U.) �l.L(—r�` '4.31 • i' i 10 .2:S
° Dissolved oxygen(mg/L) '�. 9-'1' •".t '°a 2. •3
3.25/ J
Conductivity(µmhos/cm) ^soy 20 3 • i/,
Temperature(°C) -NA,d I -.°'L•t0 "1.A.i m.4-1 AA.q IN
pH(S.U.) 3c42 q. 3_3 '4-.15 14.2.5".
Dissolved oxygen(mg/L) '1" I 7
1- •
` -8 IIMIIII
6.5% 00 2 2ct t
Conductivity(µmhos/cm) 3
Temperature(°C) - ,*1 'AA.ko A.A.i 'vA.beill tk•<j
pH(S.U.) 44 - 7 - ?.31"1 •t- t .2.0
Dissolved oxygen(ing/L) ,.'l g 11E1111 .v/ - •4.1
13% •
Conductivity(µmhos/cm) 2ciS 2'I% 283
Temperature(°C) -t.4.44i -IA.4i .0 -IA., 1S.0 MN
pH(S.U.) ;3q 1_22 '1-3L-j '4-.I2_ 22_
Dissolved oxygen(mg/L) .'1 t '-•,8 11. MEM
-1• 1-
26% —
Condudiviry(µmhos/cm) ZSCI 2 S01
Temperature(°C) •%:t• —77,.�."t "LS- ZS-0 'tt4.
pH(S.U.) R•2q 't•(c( _ ;.3t INI
• a ' .t
Dissolved oxygen(mg/L) "t.�t • IIMAIIIIItata ,.1"
52% Conductivit
y Z-r� toC
t 1
Temperature(°C) 16.0 1-A--1 1-S• ® Z.`t•-t li,•0 •S
pH(S.U.) -t.( '4.1
Dissolved oxygen(mg/L) "'t.to
Conductivity(µmhos/cm) 2 lete
100% Alkalinity(mgCaCO3/L) Lo Sat
Hardness(mg CaCO3/L) CC 6—'1
Chlorine(mg/L) 40.ID <J. 0 ...
Temperature(°C) 1.S•O ZS.0
initial Final initial Final Initial Final
SOP AT20-Revision 6—Exhibit AT20.3
I

Page 5 of 5
Environmental Testing Solutions,Inc.
Species: Pimephales promelas
Client: Andrews WWTP Date: 12-06-22
Day
(Analyst identified for each day,performed pH,D.O.and conductivity measurements only.)
I 3 4 5 6
Analyst _., f _ USC 13 c., `L-•C Lam_ zC
r ncentration Parameter
pH(S.U.) *3.ZO 1-Ito 1.1 -4.a1. -..1S "4.f - ".z(D 4 ft
Dissolved oxygen(mg/L) "'\• e R 1..'?) 1.4. .y - "N.f -1.• &
CONTROL, Conductivity(µmhos/cm) 301,4 30i 3Po 'SOU
MHSW Alkalinity(mg CaCO,/L) tal.--
Hardness(mg CaCO,/L) .b 1.11 0
I' Temperature(°C) ^.��-t1.-A,-.'L D Z -y .A
A• 6 . C Z"k•S 1..�0. t ��..4,.1 -t.�•i -vsk-to
T
pH(5.U.) -3I t1.0 -t y-• 1.a.a 1.35 -q-20 q-3O '4..(0
Dissolved oxygen(mg/L) .1..C1 1'k" `t.c 4.4
-1- ,• ( n3.2s%
302 •�
Conductivity(µm , 3D,1 2gathos/cm) -y mot, ( y g �` -y Temperature(°C) _'�-1 -v.k.(o -I,A- g �^A.-d -a.A• 6 L:'t•4 'IA• % 1-40
pH(S.U.) q.31 1'/•11 30 --IV, 7.3i(- �Z..o -4eJ0 -4_0q'
Dissolved oxygen(mg/L) .1Q• ! 1 .0 - "7,, ,.o., -1,' .• ..
6.5%
Conductivity(µmhos/cm) 2cco 0 3(( 3 ot., u.1Zt1z er ZctS
Temperature(°C) Z�-1 1..�1•L, -NA•R -1.A•S 1.`i.41 1-14•y -Lot.J "L`l•S
pH(S.U.) Z .p _ 1-• 1 _ 1.11 i. 3 -4.1- . O "4.0•
Dissolved oxygen(rng/L) ^1,.q -IA 0 S''.O 'g 4.' ',.'S •-•e • I .
13�o Conductivity(µmhos/cm) 2 O OICys �t T oZq$ ( _ nq,1Oe1 C
Temperature(°C) ^N... g' -1.A. E1 •- .4•1 -�-4 1..�•9, 1.4.C7 61..1.3 \4Q•y`
pH(S.U.) Y.2S '-•lt0 1.a 4, 1-14 7 '.( 1 .. .2q .U—i
Dissolved oxygen(mg/L) ,.9 g 1.. ••0 q .1 Y -1 • .
1 26% T
Conductivity(µmhos/cm) 743 a la. Qgq ,•
Temperature(°C) --NA- ' •t e
pH(S.U.) --2
Dissolved oxygen(mg/L) e D
52% C`SC `9-2
Conductivity(µmhos/cm) ZLICCI 1
2.
Temperature(°C) ,.,..k..4 -y,6
i
pH(S.U.) �i
Dissolved oxygen(mg/L)
Conductivity(µmhos/cm( .201
100% Alkalinity(mg CaCo,/L) — k,,,C*j
Hardness(mg CaCO,/L) S
Chlorine(mg/L) G O.ID L_
Temperature(°C) 14.0
Initial Final Initial Final Initial Final Initial Final
SOP AT20-Revision 6—Exhibit AT20.3

•
fe4-µ, iz; 351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Enviro n mental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: 'Earth Environmental Services NPDES#. NC0020800 Pipe#: 001 County: Cherokee
Andrews WWTP Purchase order:
1
Species: tmephales promelas Effluent dilution: 3.25,6.5, 13,26,52%
Test type: hronic Definitive Parameter code: THP6C
iSample in prmation: (to be completed by sample collector)
Comp rite sample: Sample location:
Star,date: We-//ZZ Time:j :3(j,QNf Volume collected for testing: / C 4.//,./
k End te: j Z/5/2 Z Time: O8:3D,4p Number of containers filled for testing: /
Nurnler of samples per hour: Method of transport to laboratory: Co u r+'e r
Chilli during collection? Comments: i
If chi ed,specify temperature: t
T pie rinse sample container with sample before filling. Completely fill the sample container with no air space. t
i,
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample cu `.ody: (to be completed by sample collector and facility personnel)
' p{
Sample c011ected by:
_.,„c -...r VE./37 7.2- I
Print Signature Due and time I
ille
#. RelinquiSLd by: Received by:
e. L
Signature Date and time 1
Print
Prim Sigmture Date and time
Relinqui, d by: Received by:
I - — M i
-1 k.
Pnnt Signature Date and time Print Signature Date and time
Sample re:aipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
\1,i4 f ..C:6(0r„1N- [1 :5z.M4 ` t I/` 1152-
1 Print Signature Date and time
Print Signature Dam an3 Gmc
Cusli dy seals intact?: - - ® Sample temperature upon receipt at ETS(°C):
Y D de IC
es No Not ued
Sampils received in good condition?: IS 1-1
Yes No Total residual chlorine upon receipt at ETS: _
N A (DPD Presence/Absense Indicator,MDL=0.10 mg/L) Pre'ent Ab'ent
Tra ' g number: �� ?'
Project number: 11`I'S Sample number:�!205.u2
Commglr is:

et
351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
I
Envirot'nental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: i arth Environmental Services NPDES#: NC0020800 Pipe#: 001 County: Cherokee
Andrews WWTP Purchase order:
7
Species: Aimephales promelas Effluent dilution: 3.25,6.5, 13,26,52%
Test type: Chronic Definitive Parameter code: THP6C
Sample information: (to be completed by sample collector)
Com toSampleEl:RUMt- i
Po�' sample: location:
ti
Start gate: Time: 33e 4r1 Volume collected for testing: l 6cU10 rt
End crate. 1 Time: 4Q"—il 4M Number of containers filled for testing: I
i
Numl►er of samples per hour: 14-frtN (4 Method of transport to laboratory: c 0,1 Pi e C
Chillelld during collection? 3' C \(P' Comments:
If chirllkd,specify temperature: -3t— ( I
TIple rinse sample container with sample before filling. Completely fill the sample container with no air space.
I Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. t
a i
i g'
1
3
il
Sample cos-ody: (to be completed by sample collector and facility personnel)
1 Sample cc'ected by:
1 i
, ll:5a2,...,.x (&,,,,,,4 44
rs
6.�m Signature Date and time
d 6 is u Relin
q F by: 1 Received by: QlC.e.„1/P
!, MILK t' Idff14 1
u t�fr Y — /��k�
I Pnn! Signature D to and time Print .I MUIC Date and time
Relinquished by: Received by:
— --
1. Prim Signature Date and time Porn signrturc Date and time 1
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
IJ4&D / 47d 1 i1
IX (2. •) 2
Z
Print Jr Signature Date and time Print Signature Date and time
Cus oly seals intact?: — — Sample temperature upon receipt at ETS(°C):
IYes Nor usd 1 l—
Sam.1.s received in good condition?: IN
Yes No Total residual chlorine upon receipt at ETS: _ 1
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) Pr''''' Able"t
Tratkng number: O
Project number Sample number: 12021961.2D
comm.'s:

351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
._ Envir• mental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility arth Environmental Services NPDES# NC0020800 Pipe#: 001 County: Cherokee
ndrews WWTP Purchase order:
1
Species: 1'imephales promelas Effluent dilution: 3.25,6.5, 13,26,52%
Test type: t hronic Definitive Parameter code: THP6C
1
Sample i i rmation: (to be completed by sample collector)
Comps ite sample: Sample location: l.tt'✓\.[—
Sta ate: Q i j{1 Time: d;(�-) Volume collected for testing: t tort
End 1:te: Time: Number of containers filled for testing: ' .
1 Nu .-r of samples per hour: Apperk I 4-
Method of transport to laboratory: ef31•c��
Chil':9 during collection? Comments: 4
If c ' ed,specify temperature: aUL
ple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory. li
1
Sample c . ody: (to be completed by sample collector and facility personnel)
0
Sample f leered by: „
IAA4._
ItPo Signawre Dale and time Pi
$ Relinqu d by: j Received by:
a
41902 L /a p. VgAt:e;,-,..---:, j 1
�)j/ ter, r.
Prim Signature ,{ Date and time Pont Signature Date and time
1 Relinqui. -d by: _ J Received by:
..., .,
P z{ � //. Z)
Prim Signature Date and time Print Signature Date and time
Sample rePipt information: (to be completed by ETS personnel)
Relinqui li .to ETS by: Received at ETS by:
i it
Pont Signature Datc and time Pont Signature Date and time
Cus .y seals intact?: - _ S, Sample temperature upon receipt at ETS(°C):
Yes No Not used 4 .3'C
Sam s received in good condition?: lall -
N°IN Total residual chlorine upon receipt at ETS: _ ��
(DPD PresenceiAbsense Indicator,MDL=0.10 mg/L) Present Absent
Tra ng number: N
Project number: 114S7 Sample number: 1:1,(1 OC•Otc-
Comm s:
i —

s
,, Pimephales promelas
Chronic Reference Toxicant Control Chart
r Environmental Testing Solutions, Source: In-house Culture
Inc.
0.9 1 1 I 1 1 1 I 1 I I 1 I I I 1 I I 1 1 1
- Control Limits (± 2 Standard Deviations) -
0.8 — —
• •
_ • •
0.7 • • —
,ea,�..._ • .,
•-- — _- ----- .
- • • • • • •..
• -
_1 0.6 —
t;,lo
I I I 1 I I I I I I I I I I I I I I I I
N
UN 1.2 I I 1 I I I I I I I 1 I I I I 1 I I I I
Warning Limits
ri 1.0 - -
• •
- •
! _fi •, • ..• -- , •: . , �..� �•.---- .�y :,nm�—. �...�. -
•
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
0a 1.1 13.0.03.21 1a i>05:L1 O :IN O�y1 0�_l-0$IT 0$1-ti OS l-03al-O�al- 1 al-1 al-O al- 13al-OQ IT 0a 2'L 06al-
06 01 0'6 09- 10- 11 11,. 01- 01. 03" 0p' OS- 00 0�' 0�' 0$. 09' %.0 %). NI'
I
Test date
• 7-day IC25=25%inhibition concentration. An estimation of the potassium chloride concentration
which would cause a 25%reduction in Pimephales growth(calculated using ToxCalc).
1 Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
- - _ — Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
-.,. .a USEPA Warning Limits (mean logarithmic IC25±SA 75 converted to anti-logarithmic values,
SA75=75th percentile of CVs reported nationally by USEPA)
re.ea a:
xerewea
cy
1r Sumner
1

}
r AV19
PO Box 7565
' Asheville, NC 28802
I Phone: [phone]
Envuonmental Testing Solutions.Inc. Fax: [phone]
I
1
i
I
IU)`arch 19, 2023
s. Wanda Ladd
arth Environmental Services, Inc.
t Bison Lane
Murphy, NC 28906
TS Project Number: 17693
Est Start Date: March 07, 2023
Facility Tested: Andrews WWTP
i
Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc.
Para meter
GCode Test Procedure, Method Number Result (ChV)
THP6C Fathead minnow, Pimephales promelas,Larval Survival and Growth Chronic
EPA-821-R-02-013, Method 1000.0 >52/0
AI'ltoxicity testing results required as part of your permit must be entered on the Effluent Discharge
ionitoring Form (MR-1)for the month in which it was performed, using the parameter code THP6C.
dditionally, the DMR Reporting Form AT-5 must be signed and e-mailed or mailed to the following
a dress:
ATForms.ATB(a�ncdenr.gov
North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
' 1621 Mail Service Center
' Raleigh, NC 27699-1621
Pl.ase call if you have any questions concerning these results.
Si 4 erely,
i ,...---
Jill Sumner
L.i
oratory Supervisor
North Carolina Certificate Numbers: Biological Analyses: 037,Drinking Water: 37786,Wastewater: 600
South Carolina Certificate Number: Clean Water Act: 99053-001
1
l

,
,,,- - PO Box 7565
Asheville,NC 28802
Phone: [phone]
- Fax: [phone]
Environmental Testing Solutions Inc.
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date: March 19,2023
Facility: Earth Environmental Services,Inc. NPDES#t: NC 0020800 Pipe a: 001 County: Cherokee
Andrews WWTP
Laboratory Performing Test: Environmental Testing Solution nc. Ce ficate#037 Comments:
Signature of Operator in Responsible Charge(ORC):
G
ORC Phone/E-mail: 57 g -S 5,.- 430 - • r W; Project#: 17693
Signature of Laboratory Supervisor:_- Sample#: 230306.02,230308.26,230310.12
e-Mail to: [e-mail] Test Organisms: Pimephales promelas
Or Mail Original to: North Carolina Division of Water Resources Supplier
Water Sciences Section/Aquatic Toxicology Branch In-house Culture
1621 Mail Service Center Begin hatch: 03-06-23 1425
Raleigh,NC 27699-1621 End hatch: 03-07-23 0500
Test Start Date: March 07,2023
Replicate number
1 2 3 4
Control Surviving number of larvae 10 10 10 9 Survival(%) 97.5
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.752
Weight/original(mg/larvae) 0.777 0.737 0.753 0.742 Average wt/ 0.773
surviving(mg)
%Effluent Surviving number of larvae 10 10 10 10
3.25% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.657 0.741 0.726 0.833 Average wt(mg) 0.739
%Effluent Surviving number of larvae 10 10 10 10
6.5% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.626 0.688 0.730 0.724 Average wt(mg) 0.692
%Effluent Surviving number of larvae 10 10 10 10
13% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.763 0.759 0.691 0.757 Average wt(mg) 0.743
%Effluent Surviving number of larvae 10 10 10 10
26% Original number of larvae 10 10 10 I 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.708 0.702 0.803 0.800 Average wt(mg) 0.753
%Effluent Surviving number of larvae 10 9 10 10
52% Original number of larvae 10 10 10 10 Survival(%) 97.5
Weight/original(mg/larvae) 0.689 0.697 0.670 0.660 Average wt(mg) 0.679
Water Quality Data
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6
Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final
Control
pH(SU): 8.16 7.89 8.01 7.75 8.04 7.56 7.92 7.83 8.16 7.74 8.07 7.83 8.04 7.93
DO(mg/L): 7.8 7.9 7.9 7.2 7.6 6.9 7.6 7.3 7.8 7.3 7.9 7.8 7.9 7.4
Temp.('Cl: 24.8 24.5 24.8 24.8 24.7 24.6 24.8 24.6 24.6 24.7 24.7 24.6 24.8 24.5
High Concentration
pH(SU): 7.98 7.82 7.99 7.72 7.91 7.49 7.94 7.86 7.87 7.62 8.00 7.77 8.08 7.85
DO(mg/L): 7.8 7.8 8.0 7.4 7.9 6.4 7.8 7.4 7.8 7.1 7.9 7.6 7.8 7.4
Temp.(°C): 24.9 24.6 24.7 24.8 25.0 24.4 24.9 24.6 24.7 24.5 24.8 24.5 25.0 24.6
Sample Information Sample 1 Sample 2 Sample 3 Control Analyses Survival Growth
Collection start date: 03-05-23 03-07-23 03-09-23 Qyzimi Normal: Yes Yes
Grab: • 11 Horn.Var. Yes Yes
Composite duration: 24.25-h 24-h 24 5 h 1 cz NOEC: 52% 52%
'�sde
Alkalinity(mg/L CaCO3): 61 69 67 59-62 LOEC: >52% >52%
Hardness(mg/L CaCO3): 74 70 68 91-92 ChV: >52% >52%
Conductivity(µmhos/cm): 223 237 234 290-309 Method: Visual Insp. Dunnett's
Total residual chlorine(mg/L): <0.10 <0.10 <O10f
Sample Temp.at Receipt(°C): 2.8 2.0 3 2 _'",�+,-,,itZ1qt Survival Growth
%Effluent Critical Calculated Critical Calculated
3.25% . 2.410 0.404
6.5% 2.410 1.872
13% . . 2.410 0.303
26% . . 2.410 -0.031
I Overall Analysis: 52% . 2.410 2.276
Result: PASS LOEC: >52% NOEC: 52% ChV: >52%
DWR Report Form AT-5

7wt a ""Wr .,# ,> Page 1 of 5
EnvironmentaiTesting 5o1utions,Inc.
,
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephales promelas
Client: Andrews WWTP County: Cherokee
NPDES#: NC 0020800 Outfall#: 001
Project#: VIVO)
Dilution preparation:
Dilution prep(%) 3.25 6.5 13 26 52 Sample was not aerated or treated unless otherwise
Effluent volume(mL) 32.5 65 130 260 520 noted on this form. Sample was warmed to 25.0±1.0°C
Diluent volume(mL) 967.5 935 870 740 480 in a warm water bath and then diluted to the test
concentrations with moderately hard synthetic water
Total volume(mL) 1000 1000 1000 1000 1000 (MHSW).
`
Test organism information: Test information:
Organism source: In-house culture Randomizing template: Lklt?,.
Incubator number Age: <24-hours old 1C.shelf location:
Spawn date: cr -pt•15 Artemia CHM number: CHM1222
U14-01.-13 t'1..S Drying information for weight determination:
Hatch dates and times: -r crb-6t•"1.-S 0 S OQ Date/Time in oven: ay. c i.r
*Initial oven temperature: ‘,DO•C._
Transfer vessel information: pH(S.U.)= a•kS Date/Time out of oven: cr,-tt,ts Crab
Temperature(°C)= '1-{.A *Final oven temperature: 4.00•C
Average transfer volume(mL): <0.25 mL Total drying time: 14440askS
i *60°C Oven,Thermometer SN:14-985B5
r oily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation,renewal, Sample MHSW
or termination number batch used
Time Analyst I Time Analyst Time Analyst used
0 03-07-23 oSIA 4 `IIO ,A011 1SoI ob.Ct OAle-tsA
1 03-08-23 OSbO �( 1 OO cT"t- y� L�'
2 03-09-23 U
OS00 ItC0 1xx, TV-LA) V 13krS0a as. 1n-of-1"b
3 03-10-23 US00 0U0 4 V` 0-11to �{
4 03-11-23 (�i
oL�0o � 1Z.00 (A Od IO y -1.3v3!0.1'L 03•06-1.SA
5 03-12-23 Ub00 I^ 1Ice Ii{ o&iO It 1
6 03-13-23 OSOO 1100 _ �110 11 1
7 03-14-23 Cr,
ObZq fik-
Chemical analyses:
Parameter Reporting Limit _Method number Meter _ Serial number
pH 0.1 S.U. SM 4500-Hi-B-2011 Accumet AR20 93312452
Dissolved Oxygen(D.O.) 1.0 mg/L SM 4500-0 G-2016 YSI Model 52CE 18D104324 —
Conductivity 14.9 µmhos/cm SM 2510 B-2011 Accumet AR20 93312452
Alkalinity 5.0 mg CaCO3/L SM 2320 B-2011 Accumet AR20 93312452
Hardness 5.0 mg CaCO3/L SM 2340 C-2011 Not applicable Not applicable
Chlorine,Total Residual 0.1 mg/L ORION 97-70-1977 Accumet A6250 92349123
Temperature 0.1°C SM 2550E-2010 Digital Thermometer t-S0b6li6SS
Control information: Acceptance criteria Summary of test endpoints:
%Mortality: 1.5 7 S 20% 7-day LCso(%) ) S 2-7,
Average weight per initial larvae: O,—IS^2- _ NOEC(%) 52 7.
Average weight per surviving larvae: 0--1-13 >_0.25mg/larvae LOEC(%) y S2.7.
ChV(%) )527.
IC25(%) >S2.7a
SOP AT2O-Revision 6—Exhibit AT20.3
a
I

Page 2 of 5
Environmental Testing Solutions.Inc.
Species: Pimephales promelas
Client: Andrews WWTP Date: 03-07-23
Survival and Growth Data
Day CONTROL 3.25% 6.5%
A BCD E F G H I J K L
0
10 10 10 10 10 10 10 10 10 10 10 10
1
to Ip 10 ID ►D (D (0 10 / 0 to to /0
2
ID 10 10 ID ID ID ! b ID ID ID ID ID
3
10 10 CO PO 10 /p ID 10 10 /0 I0 l0
4
/ 0 ID 10 10 ID 10 10 10 10 / 0 ID ID
5 10 1D /0 ID l0 / 0 10 10 / 0 /0 ( 0 /0
6 1p (0 Io /0 10 I (0 (D (p rt> (0 (O
' t p (o (0 '04 (0 (0 10 to (0 to to (0
*A=Pan weight(mg)
Tray color code: 01(evy
Analyst: et. )t1..5) 14.7/Itf I,5 15.3o ttt.L3 (5.36 15.75 t3. 32 I`¢:SS 14..ss.? i e.69 13.61
Date: 02-22-23
*B=Pan+Larvae weight(mg)
ADate:nalyst:
0 3-1 G bL 22.2522.14 22./Y 22.7121.a0 22.7723.01 y1.45 21.1 t 21.70 21.1920.BS
-2
C=Larvae weight(mg)=B—A
1.11 —On 1.S S '1.`l"L 1.•S"1 sil 1.1s. S.35 UJ. o✓. & 8 1.30 7•i44
Analyst: its
Weight per initial number of larvae(mg)
I =C/Initial number of larvae
M �� ati ham � f� % 0
^� �a
Analyst: cc p" 0• 0• O• a O• 4 0•
Average weight per initial Percent
number of larvae(mg) reduction from 0- 1S2— 0-7'i Li 7_ O.I,12.. 3.0 7.
control(%)
*Weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520.
Comment codes: c=clear,d=dead,fg=fungus, k=killed, m =missing,sk=sick,sm= unusually small,
Ig=unusually large,d&r=decanted and returned,w=wounded.
Comments:
SOP AT2O-Revision 6—Exhibit AT20.3

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a EnvironmentafTesting Solutions,Inc
Quality Control: Verification of Data Entry,Calculations,and Statistical Analyses
Chronic Whole Effluent Toxicity Test(EPA-821-R-02-013,Method 1000.0)
Species: Pimephales promelas
Entered by.
Facility: Andrews WWTP Kelley Keenan
Test start date: March 07,2023 Reviewed by,
Project number: 17693 °m 5a7"ef
Sample numbers 230306.02,230308.26,230310.12
Concentration(N) Replkate Initial number of Final number of larvae A=Pan weight lmg) B=Pan+Larvae I Larvae weight(erg) Weight/Surviving Mean weight/ Coefficient of variation Weight/Initial number Mean survival Mean weight/ Coefficient of variation Percent reduction from
larvae weight(mg) =B-A number of larvae(mg) Surviving number of 10..nwayateetrv..Ato5ower, of larvae(mg) (%) Initial number of (NI control(%)
larvae(mg) nrurv.ei(%) larvae(mg)
A 10 10 14.51 22.28 7.77 0.777 0.777
B 10 10 14.79 22.16 7.37 0.737 0.737
Control 0.773 4.9 97.5 0.752 2.4 Not applicable
C 10 10 14.65 22.18 7.53 0.753 0.753
9 10 15.30 22.72 7.42 0.824 0.742
E 10 10 14.63 21.20 6.57 0.657 0.657
F 10 10 15.36 22.77 7.41 0.741 0.741
3.25% G 10 10 15.75 23.01 7.26 0.726 0.739 9,8 0.726
100.0 0.739 9.8 1.7
H 10 10 13.32 21.65 8.33 0.833 0.833
1 10 10 14.85 21.11 6.26 0.626 0.626
1 10 10 14.82 21.70 6.88 0.688 0.688
6.5% -._ - 0.692 6.9 100.0 0.692 6.9 8.0
K 10 10 14.69 21.99 7.30 0.730 0.730
L 10 10 13.61 20.85 7.24 0.724 0.724
M 10 10 15.41 23.04 7.63 0.763 0.763
N 10 10 13.11 20.70 7.59 0.759 0.759
13% 0 10 10 13.93 20.84 6.91 0.691 0.743 4.6 0.691 100.0 0.743 4.6 1.3
P 10 10 14.06 21.63 7.57 0.757 0.757
Q 10 10 14.77 21.85 7.08 0.708 0.708
R 10 10 15.50 22.52 7.02 0.702 0.702
26% 0.753 7.4 100.0 0.753 7.4 -0.1
S 10 10 14.83 22.86 8.03 0.803 0.803
T 10 10 13.88 21.88 8.00 0.800 0.800
U 10 10 13.53 20.42 6.89 0.689 0.689
✓ 10 9 14.60 21.57 6.97 0.774 0.697
52% - 0.698 7.5 97.5 0.679 2.5 9.7
W 10 10 15.76 22.46 6.70 0.670 0.670
X 10 10 13.44 20.04 6.60 0.660 0.660
Outten 001: MSD= Minimum Significant Difference
Dunnett's MSD value: 0.0776 PMSO= Percent Minimum Significant Difference
PMSD: 10.3 PMSD Is a measure of test precision.The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA(10th percentile)=12%.
Upper PMSD bound determined by USEPA(90th percentile)=30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile,respectively,of PMSD data from EPA's WET Interlaboratory Variability Study(USEPA,2001a;USEPA,2001b).
USEPA. 2001a,200113.Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix.EPA-821-8-01-004 and EPA-821-8-01-005.US Environmental Protection Agency,Cincinnati,OH.

_ Entered by:
�Kelley Keen
xi
Analyses 4Rev.rned by.
l,m Sumner
Environmental Testing Solutions,Inc.
0`
Larval Fish Growth and Survival Test-7 Day Growth
Start Date: 3/7/2023 Test ID: PpFRCR Sample ID: Andrews
End Date: 3/14/2023 Lab ID: ETS-Envir.Testing Sol. Sample Type: DMR-Discharge Monitoring Report
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas
Comments:
Conc-% 1 2 3 4
D-Control 0.7770 0.7370 0.7530 0.7420
3.25 0.6570 0.7410 0.7260 0.8330
6.5 0.6260 0.6880 0.7300 0.7240
13 0.7630 0.7590 0.6910 0.7570
26 0.7080 0.7020 0.8030 0.8000
52 0.6890 0.6970 0.6700 0.6600
Transform:Untransformed 1-Tailed Isotonic
Conc-%, Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean
D-Control 0.7523 1.0000 0.7523 0.7370 0.7770 2.367 4 0.7523 1.0000
3.25 0.7393 0.9827 0.7393 0.6570 0.8330 9.796 4 0.404 2.410 0.0776 0.7393 0.9827
6.5 0.6920 0.9199 0.6920 0.6260 0.7300 6.900 4 1.872 2.410 0.0776 0.7293 0.9694
13 0.7425 0.9870 0.7425 0.6910 0.7630 4.636 4 0.303 2.410 0.0776 0.7293 0.9694
26 0.7533 1.0013 0.7533 0.7020 0.8030 7.405 4 -0.031 2.410 0.0776 0.7293 0.9694
52 0.6790 0.9026 0.6790 0.6600 0.6970 2.502 4 2.276 2.410 0.0776 0.6790 0.9026
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-Wilk's Test indicates normal distribution(p>0.01) 0.97945 0.884 -0.0344 0.32989
Bartlett's Test indicates equal variances(p=0.16) 7.89765 15.0863
Hypothesis Test(1-tail,0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df
Dunnett's Test 52 >52 1.92308 0.07755 0.10309 0.00419 0.00207 0.12345 5,18
Treatments vs D-Control
Linear Interpolation(200 Resamples)
Point % SD 95%CL(Exp) Skew
IC05 33.561 12.818 0.000 44.742 -0.7810
IC10 >52
IC15 >52 1.0 -
IC20 >52
0.9-
I IC25 >52
IC40 >52 0.8
IC50 >52 0.7-
0.6-
to
c 0.5-
0
a 0.4-
Os
10.3-
0.2-
0.1-
O 20 40 60
Dose%
Dose-Response Plot
0.9
0.8
0.7 _ 1-tail,0.05 level
of significance
0.6
0 0.5
0
0.4
0.3
0.2
0.1
0
O u N N
ti

11 54 Page 4 of 5
Environmentallosting Solutions,Inc.
Species: Pimephales promelas Date: 03-07-23
Client: Andrews WWTP
Daily Chemistry:
Temperatures performed at the time of test initiation,renewal or termination by the analyst identified in the Daily Renewal Information table
located on Page 1. Alkalinity,hardness and chlorine(total residual)performed by the analyst identified on the bench sheet specific for each
analysis and transcribed to this bench sheet.
Day
(Analyst identified for each day,performed pH,D.O.and conductivity measurements only.)
0 1 2
Analyst A
Concentration Parameter �
pH(S.U.) gm, '4.e9 1 .o�e 3. 1.5tp
Dissolved oxygen(mg/L) .@ '.� A 7,I 1.2 44 LO
CONTROL, Conductivity(pmhos/cm) �(,qO 301 ?ot3
{ MHSW Alkalinity(mg CaCO3/L) SA ' -----.._ atSi
Hardness(mgCaCO3/L) Pct.. erV IA.'an 4t-L--
i Temperature(°C) A.: -g Z.` .-5 li-k•8 -1.A. (2 �ZU°t--1 Z�•10
C1 pH(S.U.) OU -Y•614 0U2 I l. " e `1)496 qs-6
Dissolved oxygen(mg/L) '16 /)... iq ;,2- F3 O U..g
3.25% y,,
Conductivity(pmhos/cm) Sea. Jog qe .
Temperature(°C) 1`tL. ' -LA•, Z`'L t c ^o-t. S. " k. ' -124.ti
t7 pH(S.U.) .'Z "141 1'J 8/sp 1• • 4,c9 _
Dissolved oxygen(mg/L) "',- IAA �� ,Z. ✓•o LI.8
6.5% 6 Conductivity(µmhos/cm) `3VI r� 3 t,p a ... 29 'e
Temperature 1°C) y'•�\\,.•6 /-1...k- e 't�• \ -v k./� IA.
. 4 1l-L
pH(S.U.) CE,0-+I' 7,DS "1.48 #1,`1V/ M.02. 7•��
Dissolved oxygen(mg/L) -'.. � !y,"d , 61Z-
0.0 u.8
13% a9 Z 'k)2. �C
Conductivity(µmhos/cm)
I Temperature(°C) LA•4\ 'IA-"1 -A.1 -A.A.S -1_4..6 /-� t.10
pH(S.U.) 0.03 ' ..Q( `&Ot 4.1c /7•iq -,s
Dissolved oxygen(mg/L) 1•3- '1-.8 r}�{g 4J,k4 t .0 iO•kA
26% (p ) 2® 2. Dd ._ --. 63�
Conductivitymhos/cm ^1 y,;,,
Temperature(°C) "L .1 A-4- 1 IA•1 �"L'I•S _.. Z"1.1 1.9-6
pH(S.U.) 'I.% ,.7 . i I 3 °4 I 0 /1 `4�
Dissolved oxygen(mg/L) �•,� I, _� �,� -7 ,
52% Conductivity(µmhos/cm) A a1,0
Temperature(°C) /�'\`1.• O A4• J S.0 -1.4-4
pH(S.U.) L"l 3 'j' .
jy$
Dissolved oxygen(mg/L) •
Conductivity(pmhos/cm) 221 1 _ 41
100% Alkalinity(mg CaCO3/L) L t ke
Hardness(mg CaCO3/L) .-VA —10
Chlorine(mg/L) •C Q, 1 t7 '(0.10
Temperature(°C) -IS•D 15•Q
Initial I Final Initial Final Initial Final
SOP AT2O-Revision 6-Exhibit AT20.3
I

• 1
., `art"'`-'' , , .t Page 5 of 5
Environmental Testing Solutions.Inc.
Species: Pimephales promelas
1 Client: Andrews WWTP Date: 03-07-23
Day
i (Analyst identified for each day,performed pH,D.O.and conductivity measurements only.)
3 4 5 6
Analyst VC
COT { 65t qs. L 9L� $L ‘A-- Q
ntentration Parameter Q-1 C O n
I pH(S.U) lryy q-D3 0•tt0 4-1T s c L. T 3 I.9q- 1._`p
3
1
iDissolved oxygen(mg/L) ,)-V(41 NMI -4-5 3 A-9 , MRE 1•q
CDNTROL, Conductivity(µmhos/cm) 3011 MI a99 a99 30 '
MHSW Alkalinity(mg CaCO3/L)
Hardness(mg CaCO3/L) ••,, , wimheril
Temperature(°C) Z�`. 'AA-- 1� 1
. -b l`'1-9\ ��• Z`k•10 1..-k. 1.�•S
/� H S c -t 3'1 p dye �/' G
PH(S.U.) 7'Y7-1 �•U 1•�� 7-YT O•l7tP 17 j 'O� �•-13
Dissolved oxygen(mg/L) 1143 4.4 S 1-.3 ® /1 }
13.25%
II Conductivity(µmhos/cm) ' d`i5 _ _ Al __t, ,{ 3 07
Temperature(°C) -1.%�.1.JJ-k �j-R 'op A-0 -(--A.O o-IA• 1 -t 1 --k.1. I 7-A.-
1 pH(S.U.) i - -1. � O-os nt/ O.ii�'. .06 Mill
-
i —1 Q
Dissolved oxygen(mg/L) �•.� 1.4 .O -q-a -4-g = 6
6.5% 3 p —
Conductivity(µmhos/cm) 302. G �l .0s A9q 30 -
Temperature(°C) �'t�. D 1.k. J A'A• 1 AA-i., -IA.1 "14.1 -1_`11CI' _4 .1\-
1 pH(S.U.) ' (O 1'3'3 S.o4 "4.11 -311- ?.77 .0 - -1• gq
i Dissolved oxygen(mg/L) 1-6 �.,-i- 4. NIE 1,53 �1 1. —�-
i3�s ) �qq ass aR4 300
Conductivity(µmhos/cm
1 Temperature(°C) -).-A-`k Q��_l\• 1 --t(�-\- \ --/AA•(c p 1.1�\, k -Z�•�1 �.Lt- S 1.�`-50
I pH(S.U.) '\ors UPS •7.-i tJ 7•L0 J.�.JC. 7 7G f(.0$ -..&
•
Dissolved oxygen(mg/L) 1%-e 3.L+. -,a �.,3 .�j MOM -,.
26% -21Z. a-1 a43 �!
Conductivity(µmhos/cm) -
4 7 IAA.
Temperature(°C) "-VA• C -7 L'1t-6 y -LA- 9 -1_,-k.-1_,-k.•� G -IA.g • ZS.o IAA.
P pH(S.U.) r}:`I Ol0 -1 - 6.a D•op y.Dk" . 25
o Dissolved oxygen(mg/L) .® 1-K I-,S 3., '•1 •,' In..6 1.y
52io 2?`�` `, a lei+ - '0 2eLt
Conductivity(µmhos/cm) -
Temperature(°C) �l.e, L .b .•-\. i 1.-`•S Zq.Q -IA.5 -Is .0 Z'1•kc,
pH(S.U.) - 4:4 to
Dissolved oxygen(mg/L) 4.S
•
1 Conductivity(µmhos/cm) a - -
00% Alkalinity(mg CaCO3/L) (01
Hardness(mg CaCO>/L) \D
Chlorine(mg/L) . Q.I 0
Temperature(°C) -IS-0
Initial Final Initial Final Initial Final Initial Final
SOP AT2O-Revision 6-Exhibit AT20.3
1
i

I
1
351 Depot Street
1's, Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environ—lental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: k irth Environmental Services NPDES#: NC0020800 Pipe#: 001 County: Cherokee
Andrews WWTP Purchase order:
Species: Iimephales promelas Effluent dilution: 3.25,6.5, 13,26,52%
Test type: Chronic Definitive Parameter code: THP6C
Sample in.lwmation: (to be completed by sample collector)
,, Compote sample: Sample location: 'd u. +-
Start date: " Time: (03O401 Volume collected for testing: I 6 I I
End date: Time: 1040 - ) Number of containers filled for testing:
Numy,tr of samples per hour: i pp /Lf Method of transport to laboratory: COu(i° r t
Chiller during collection? 1.1f—c a"C Comments:
If chilled,specify temperature: / a tic I
q Tnple rinse sample container with sample before filling. Completely fill the sample container with no air space.
a {Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
I
)
F.
•
Sample custody: (to be completed by sample collector and facility personnel) `
1
Sample collected by:
i MD*C- 3/&/ —3
ll
(' i Pnnt signal°re wte ana t;me
Relinquished by: Received by:
P
MQU ' 3`b /2-3 0- h✓l C, 7 v/Z3 i
Print v Sicnanire Date and time Pant / Signature Date and time
r.
Relinquished by: Received by: �
k,
kr,44.01 ,
li
Prim Signature Date and times Prim Signnture• Da:,and ti m`
t
Sample receipt information: (to be completed by ETS personnel)
i
Relinquished to ETS by: Received at ETS by:
Fs........,es.„6.ro......v_ mits © �23//�10:44:,4____Lici/o
Date and time Print Signature Date and time
Cus ody seals intact?:`ct - - ® Sample temperature upon receipt at ETS(°C):
Yes No�i used �e aL
Sam.Its received in good condition?:
Ye` No Total residual chlorine upon receipt at ETS: _ 11-71
1 \ (DPD Presence/Absense Indicator,MDL=0.10 mg/L) Pre"'n Abaent
v
Tres.4Jng number: Ik
Project number:11V 3 Sample number:A3036(/•02-
Comm:Jrs:

fit
ems= 351 Depot Street
,- -,, Asheville,NC 28801
Phone: [phone]
Fax: [phone]
_ Environinental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: tY srth Environmental Services NPDES#: NC0020800 Pipe# 001 County: Cherokee
Andrews WWTP Purchase order:
Species: 'finephales promelas Effluent dilution: 3.25,6.5, 13,26,52%
Test type: Chronic Definitive Parameter code: THP6C
_, bay
Sample in'grmation: (to be completed by sample collector)
Compolrte sample: Sample location: i1 -.t I' 1
Start dpte: I"7f j End date: j) { 3 Time: 4.t, f. S1 iv! Volume collected for testing: i (..L.,:-e.; it G!1
Time: (2 2.4 Allel Number of containers filled for testing:
Num tr of samples per hour: pr'o A, j 1-4 Method of transport to laboratory: e, LA iNi e
Chiller during collection? v Comments:
If chilled,specify temperature:
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. j
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
i
t ,
Sample cuspody: (to be completed by sample collector and facility personnel)
Sample collected by: �,� 1
i hex-t}-t .i ,0,1 1 '1... C��lk'i AM t
Print Signature Date and time $
Relinquished by: / Received by: R
k i,'
k Pnnt t Signature �) Date and time Poet Signature Date and time
RelinquijIled by: Received by:
i
0Print Signature Date and time Print Signature Dale and time ["
Sample re e pt information: (to be completed by ETS personnel)
Rellii�n}quis" to ETS by: Received at ETS by:
(0,03.06.23-- xi,
tx_ 03,00,43
Print Signature Date and time Pnnt Signature Dare and time
k
Cus ody seals intact?: - - © Sample temperature upon receipt at ETS(°C):
Yes No Not used p(4•`
Samples received in good condition?: El
Yet No Total residual chlorine upon receipt at ETS: milr--/
_t (DPD Presence/Absense Indicator,MDL=0.10 ma/Li Present Absent
Tratlbng number: rik
zz
Project number: 1 IteCilample number:a`30 3196.2c0
Comma s:
i
i
5

I
351 Depot Street
Asheville,NC 28801
i Phone: [phone]
I Fax: [phone]
Envir•tp entalTesting Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility t arth Environmental Services NPDES#: NC0020800 Pipe#: 001 County: Cherokee
ndrews WWTP Purchase order:
Species: 'imephales promelas Effluent dilution: 3.25,6.5, 13,26,52%
Test type: 1 hronic Definitive Parameter code: THP6C
Sample i t rmation: (to be completed by sample collector)
1 i
r Com. ite sample: Sample location: C(g) 1 V. :,
i
1 i St. S.ate: /Q J'9 Time: (li(PIYli Volume collected for testing: I C :tl1Dt1 -' i L�' C .,
End+ae: 3)(Q I 3 Time: (U aZ74M Number of containers filled for testing: o,
1
r
s Nu ter of samples per hour: � (L{ Method of transport to laboratory: C OLt.e -i
4. Chit" during collection? eS Comments:
If ail i ed,specify temperature: ("E y,
iple rinse sample container with sample before filling. Completely fill the sample container with no air space. t
1 1 Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
1
1
Sample c `.,tody: (to be completed by sample collector and facility personnel)
S
Sample '.lected by:
rA '7 ' C. :3 is
1 _
<S. ,, Pont Signature ( Date and time
Relinqu ed by: b
Received by:AV! b 1. iii f l/ i / 1,4,0
P Signature Date and time Print Signature Date and time 1 '
fi Relinqu s ed by: Received by: -
k _ i,t .-_
Prins Signature Date and time Pnrn .Signature Date and time
Sampler .•ipt information. (to be completed by ETS personnel)
ti""
Relinqu ed to ETS by: A Received at ETS by:
'MI -..'"" &0 C'S 4 illb - VL 102 N
1 Print Signature Date and time Print Signature Date and time
t
CJill,.dy seals intact?: MI Sample temperature upon receipt at ETS(°C):
tYes No Noe used •�
1
Sa 1t les received in good condition?: n -
Ye, No Total residual chlorine upon receipt at ETS: _
EEr
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) E'`ea°"t At''''"
Tr c 'ng number: 0 'l
Project number:(/0/5 Sample number: / JV3i0
ik
Com ts:
I

Pimephales promelas
Chronic Reference Toxicant Control Chart
Environmental Testing Solutions,Inc.
Source-. In-house Culture
0.9 1 1 I I 1 I I I I I I I I 1 1 I I I I I
Control Limits (± 2 Standard Deviations) -
0.8 - -
• •
•
0.7 - • • • • -
• • •
• -
V •
00
I I i I 1 i I I I I I I I I I I I I I I
IA
`V 1.2
V I 1 I I I I I I I I I I I I I I I I I I
1 RI Warning Limits -
ri 1.0 — —
-
• • • • —
0.4 -
1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Og%p 10 6 11 O 11 01��Oa DL 0$ 3 oS�p 6 oS..03 6 01��Al.��1.O$OL 9AS tit OA 11 OS S. . 6 y,)0 L 01 03.01:L3
Test date
T
• 7-day IC25=25%inhibition concentration. An estimation of the potassium chloride concentration
which would cause a 25%reduction in Pimephales growth (calculated using ToxCalc).
--..�.. Central Tendency(mean logarithmic IC25 converted to anti-logarithmic values)
— -- m -{ Control Limits (mean logarithmic IC25±2 standard deviations converted to anti-logarithmic values)
-,... .. Laboratory Warning Limits(mean logarithmic IC25±2 coefficent of variations converted to anti-logarithmic values)
.. USEPA Warning Limits (mean logarithmic IC25±SA 75 converted to anti-logarithmic values,
SA 75=75th percentile of CVs reported nationally by USEPA)
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Location
Subba; i:040502
Town of Andrews WWTP
Latitu : 35°11'51"
Longi xle: 83°50'46"
Receiving Stream:Valley River North SCALE 1:24000
Strea -lass: C-Trout EIUC#: 06020002

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