NC0021156_Effluent Toxicity Report_20230331

Action History (UTC-05:00) Eastern Time (US & Canada)
Submit by Anonymous User 3/31/2023 12:08:40 PM (Supplemental Submittal)
Submit by bradley.bennett 6/27/2023 2:33:34 PM (Supplemental Info Submittal)
• The task was assigned to Wren.Thedford, bradley.bennett 3/31/2023 12:08:41 PM
• bradley.bennett assigned the task to bradley.bennett 6/27/2023 9:33:23 AM

Initial Review Information
......... ......... .......................................................................................................................................................................................................................
Updated ID#: * IMPORTANT: REVIEWER SHOULD VERIFY and revise here if necessary.
NCO021156
How should this project be handled?
File Discard
Information Submitted on 3/31/2023
Submitter Information
Name: Keith E. Lynch
Who is submitting the information?
Email Address:* [e-mail]
Submittal Information
The Division of Water Resources (DWR) is providing this site to allow for the upload of required supplemental information
associated with discharge monitoring reports (DMR). All DMR data is required to be submitted electronically through
the eDMR system and should not be submitted here. Division staff will review all uploaded documents and will only
maintain required information. Documents such as laboratory reports are not required to be submitted and will not be
maintained in the system.
Project Information:
Please supply the permit number for this project.
...............................................................................................................................
Existing ID#* Permit Number
N CO021156
Examples: NCxxxxxxx or NCGxxxxxx
Facility Name: * City of Mount Holly
County: Gaston
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1 5feb23chronictox. pdf 2.07M B
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Please provide descriptive comments about the attached information.
15 February 2023 Chronic Toxicity report.
* By checking the box and signing box below, I certify that:
o I have given true, accurate, and complete information on this form;

• I agree that submission of this Supplemental Information form is a "transaction" subject to Chapter 66, Article 40 of the
NC General Statutes (the "Uniform Electronic Transactions Act')
• I agree to conduct this transaction by electronic means pursuant to Chapter 66, Article 40 of the NC General Statutes
(the "Uniform Electronic Transactions Act');
• I understand that an electronic signature has the same legal effect and can be enforced in the same way as a written
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• I intend to electronically sign and submit the Supplemental Information form."
Full Name: Keith Edward Lynch
Signature:
�;M- 41
C.ra mr, wio
Date Submitted: 03/31/2023

Ceriodaphnia dubs Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: City of Mount Holly WWTP
Facility: WWTP
NPDES #! NCO021156
Test Reviewed and Approved By:
V 4
Robert W, Kelley, MU
President
Certification 4ES7819
Test Date: 15-Feb-23
Laboratory ID #: T64180
Test results presented in this report conform to all requirements of
Patrick D, Timms
QA/QC Officer
S'CI)HEC Ccrtiflcation #23104
NELAC, conducted under NJ LAC Certification Number L87819
Florida Dept. of Health. Included results pertain only to provided samples,
NCDENR. Certification# 022

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 01-Mar-23
Facility: Mount Holly WWTP NPDES# NCO021156 F
Laboratory Performing Test: Comments
x
Signature of Operator in Responsible Charge
X
.Signature of Laboratory Supervisor
IVIPAIL UM1110111NAL I U
North CaMling Glij2daphinla Gbraflic ass/Fall R22LgjWion Toxicity Tgal
Gaston
Environmental Sciences Branch
Div. of Water Quality
N:.C. DENR
1621 Mail, Service Center
Raleigh, North Carolina 27699-1621
Chronic Test Results
Calculated t= 0.7609
Critical Value= 2.608
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced 23 21 22 22
Adult (L)Ive (D)ead L L L L L L L L L L L L
Effluent % E=
TREATMENT 2 ORGANISMS
# Young Produced
Adult (L)Ive (D)ead
1 2 A r, r 7
E.L
20
23
1 20
1 21
1 20
21
1 20
1 21 14
1 29
qR
L
L
ILIL
_j
L
IL IL
IL
L
pH 1st sample 1st sample 2nd sample
Control 7.7 8.1 7.7 U 7.7 8,0
Treatment 2 7.8 8.3 7.8 8.5 7.71 8,3
start end start end start end
D.O. 1st sa pie 1st sa Ole 2nd sz mple
Control 8.1 8 2 8,2 8.3 8.2 8.5
Treatment 2 8.0 8.4 8.2 � �85 8.2 8.4
LC501Acute Toxicity Test
(Mortality expressed as %, combining
to This for Either Test
t�(starti teats
13-Feb-23
0%
Control
213
Control
0%
reatment 2
20.9
Treatment 2
ontrol CV
6.7%
PASS FAIL
% 3rd Brood
100%
Test Start Date
15-Feb.23
Sample 2 15-Feb-23
Camp Duration
1st
2nd
Samoa I X 24hrs,
Tax
Tax
Sample 2 X 24hrs.
Dilution
Sample
Sample
Hardness (mgtl-)
85.7
Spec. Cond. (pmhos)
313
465
485
Chlorine (mg/L)
W.
<0 05
<0,05
iple Tamp, at receipt ('C)
0 8
0.2
rvwnamy starifend start/end
LC50 = % Method of Determination control
i95% Confidence Limills Moving Average Probit High Can,;
o/. Spearman Kerber Other
alo I .E pH D.O.
22anism Tested Ceriohnia dubia
DEM Form AT-1

STATISTICAL ANALYSIS RESULTS
icility: Mount #. Jul NPDESiR NCO021156
imple ID: WWTP E170 T64180 1hate: 15-Feb-23
iboratory: I Certification 4: NCO22 E.p, Datc: 11/1/2023
Survival Data
7 Day Survival Test Used: FISHERS TEST
Control 100% Test Statistic: P= 1.000
Efflucilt 100% Critical Value: P= 0.01
PASS: The effluent does not reduce survival Of the test organisms.
Reproduction Data
Raw Data
- Test far Normality
Mean young/fenrale
Std. Dev.
Test Used: Shapira-Wilks Test,
Control 21.3
1.44
Effluent 20.9
1.24
W:
0.962
Critical Valoe;
0.884
Analysisfir Differences in Reproduetion
Test far Ham ogetreity of' Variance
Test Used: Equal Variance t Test,
Test Used: F Test
Calculated t=
0.76
F=
1.34
Critical Value=
2.51
Critical Value=
5,32
The data are homogeneous in variance
PASS: The effluent is
not chronically toxic.
parip i of A

ontrol Mortality and Reproduction byTest ,Day
source
re
1 2 3 4 5 6 7 8
_..
Total
G4 2-2
1
+3 9
11
23
VF4 2-3
2
+3+8
10
21
J8 2-2
3
+3+8
11
22
B3 2-3
4
+4+6
12
22
H10 2, 2
5
+5+7
11
23
88 2-3
6
3
+7+10
20
R4 2-2
7
+4+8
1 p
22
V5 2-3
8
+4+7
19
22
E9 2-3
9
4
+7+9
20
L4 2-2
10
+4+7
10
21
R9 2-2
11
+3+6
9
18
r1 2-3
12
4
+7+11
22
13
NIA
0
14
N/A
0
15
N/A.
0
16
N/A
0
17
NIA
0
18
N/A
p
19
N/A
0
eBn
20
N/A
p
21.3
6 /e Effluent Mortality and Reproduction by Test Clay
1 2 3 4 5 6 7 8
Total
G4 2-2
1
+5+6 14
21
V4 2-3
2
+4+7 9
20
JS 2-2
3
+4+7 12
23
B3 2-3
4
+3+7
10
20
H 10 2-2
5
+4+6
11
21
B$ 2-3
6
+4+7
9
_
20
R4 2-2
7
_
3
+6+12
21
V5 2-3
8
+3+6
11
21
9 2-3
9
4
+7+10
21
L4 2-2
10
+4+6
9
19
R9 2-2
9
+8+11
22
F1 2-3
114
+7+11
23.
0N/A
0
0N/A
0
0
15
NIA
p
0
16
N/A
0
N/A
a
NIA
p
N/A
0
Mean
N/A
0,
20,9
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JC
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22-Feb-23
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12:59 PM
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24.7
24.6
25.2
25.2
25
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Control New temp,. °C
24,6
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