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HomeMy WebLinkAbout2019 I-33 thru I-94 PonarTable 1-33. Raw data from benthic ponar grab samples collected in May 2019 at each station in all 15 creeks in the PCS study. Benthic organisms were not counted beyond 100 individuals in any sample. Taxonomic hierarchy Broomfield Creek SCUT1 control Jacks Creek Little Creek (control) Jacobs Creek PA2 control Drinkwater Creek Long Creek control Tooley Creek Muddy Creek control Huddles Cut Porter Creek DCUT11 DCUT19 control Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Annelida Clitellata Enchytraeidae sp. - 2 Glossiphoniidae sp. 33 1 - - Naididae Naididae sp. 3 Naididae sp. w/ hair 17 10 9 - Dero sp. 18 6 - 1 - 3 - - - - - 26 - 1 - 3 Naididae sp. w/o hair 163 217 283 - - 147 - 2 3 3 2 60 17 3 - - 22 26 Limnodrilus hoffineisteri 56 78 34 2 - - - - - - 4 4 - - Paranais litoralis - 4 - - - - - - - Tubificoides heterochaetus 3 - - - - 5 - 1 - 1 - 3 11 - - Polychaeta Ampharetidae Amphicteis floridus - 15 17 4 41 4 18 44 87 21 1 1 42 4 1 - 1 1 95 224 155 7 35 Capitellidae Heteromastus filiformis - - - - - - - - - - 6 - Mediomastus ambiseta 11 - - Nereididae Neanthes succinea 5 9 Spionidae Marenzelleria viridis - - 12 1 Polydora cornuta 1 - - Streblos io benedicti 4 2 62 4 37 22 35 110 12 31 18 48 22 1 1 Arthropoda Collembola Sminthuridae sp. - - - - - - 1 - - - - - - - - 4 Insecta Coleoptera Hydrophilidae Berosus sp. 1 2 5 Haliplidae Peltodytes sp. - - - - - - - - - - 3 Diptera Ceratopogonidae Bezzia/Palpomyia complex 3 17 2 3 1 1 1 2 18 14 17 28 - Chaoboridae Chaoborus albatus 33 - 6 - - - - - - Chironomidae Apedilum sp. - - - - - - - - - - - - - - 2 - - Chironomus decorus 3 21 1 11 1 38 44 148 90 485 147 491 363 402 33 486 179 421 136 264 86 356 379 9 78 406 8 6 79 11 172 1-39 Table 1-33. (continued). Taxonomic hierarchy Broomfield Creek SCUT1 control Jacks Creek Little Creek (control) Jacobs Creek PA2 control Drinkwater Creek Long Creek control Tooley Creek Muddy Creek control Huddles Cut Porter Creek DCUT11 DCUT19 control Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Chironomus stigmaterus 1 2 5 7 3 149 15 1 7 29 Cladopelma sp. 1 - - - - - - - - - - - - Clinotanypus sp. - 3 1 Coelotanypus sp. - - 37 - Cryptochironomus sp. 4 4 27 Cryptotendipes sp. - - 2 Dicrotendipes nervosus 4 1 3 12 1 73 13 17 42 3 Dicrotendipes w/ tubule - - - 6 - - - - - - Endochironomus sp. 1 1 - - - - - Goeldichironomus devineyae - 3 3 1 10 2 7 4 4 3 - Goeldichironomus holoprasinus - - - - - - - - 4 - - 3 Glyptotendipes sp. - - - 1 9 1 1 - - - Kiefferulus sp. - 7 3 - 96 - - - - - 3 8 Nanocladius sp. - - - 2 19 3 3 - - Orthocladiinae sp. - - - - - 5 - - Parachironomus sp. 4 - - 2 1 - 11 1 2 11 8 Polypedilum sp. - - 3 - - 3 - - - - - - - - - Procladius sp. 5 6 62 - 33 - 2 - - 1 - - 9 8 6 4 4 2 293 Tanypus neopunctipennis - 48 7 14 3 3 36 2 30 43 6 4 2 8 9 2 4 10 57 21 8 5 - 24 - 44 Tanytarsus limneticus - - 5 3 - 10 - 8 - - - - - - - - - - - - - - - - - Tanytarsus sp. 3 7 8 5 36 35 4 58 22 81 9 26 1 3 3 22 1 25 17 290 15 9 Hemiptera Belostomatidae Abedus/Belostoma sp. - - - - - 5 - - - Corixidae Corixidae sp. - 2 1 1 1 3 3 8 17 2 - 2 Trichocorixa calva 1 - - - - - - - - - - - Trichocorma sexcinta - - 1 - - 12 1 1 2 - 4 Odonata Coenagrionidae Enallagma sp. - 1 - 1 1 - - 5 1 1 1 2 - 5 - Libellulidae Perithemis tenera 1 - 2 - - - - - - - 2 5 3 Trichoptera Oecetis inconspicua - 2 - - Malacostraca Amphipoda Corophiidae Apocorophium lacustre - 1 - - 25 Apocorophium louisianum 1 - - 2 1 - - 1 - 8 - Apocorophium sp. - - - 1 - - 1 3 4 3 6 1 9 2 33 - - 10 - 1 - - - 8 2 - Gammaridae Gammarus tigrinus 2 1 8 10 5 255 5 28 6 11 35 7 15 11 18 6 1 4 3 176 36 134 203 4 476 58 14 Decapoda Palaemonidae Palaemonetes pugio - - - - - - - - 1 - - - - - - - - 1 - - - - - - - Isopoda Anthuridae Cyathura polita - - - - - 12 Sphaeromatidae Cassidinidea lunifrons 1 2 2 2 1 1-40 Table 1-33. (concluded). Taxonomic hierarchy Broomfield Creek SCUT1 control Jacks Creek Little Creek (control) Jacobs Creek PA2 control Drinkwater Creek Long Creek control Tooley Creek Muddy Creek control Huddles Cut Porter Creek DCUT11 DCUT19 control Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Mysida Mysidae Americamysis almyra 2 - 9 1 1 1 14 3 2 - 3 4 1 2 3 - 6 - - - - - - Ostracoda Candonidae Candonidae sp. 2 - 44 3 3 - 13 1 2 - 92 1 - - - - 125 1 - 9 - 88 6 177 158 71 - Cytherideidae Cyprideis littoralis - 2 22 1 6 4 14 1 2 - 1 3 2 34 1 - 1 38 3 2 37 18 3 4 - 219 Mollusca Bivalvia Bivalvia sp. 5 - - - - - - - - - 1 - - - - - - - - - - - - - Dreissenidae Mytilopsis leucophaeata - 3 2 4 Mactridae Mactridae sp. - - - - 1 - - Rangia cuneata 6 1 - 2 1 Tellinidae Macoma balthica - - - - - - - - - Macoma tenta - 2 2 5 1 - 1 - Gastropoda Basommatophora Physidae sp. (physa /physella) - - - - - 24 - - - - - - - - 1 - - - 1 - - 3 - Neotaenioglossa Hydrobiidae Littoridinops sp. 6 - 17 5 3 4 6 34 3 35 57 87 24 9 16 31 21 3 2 18 39 6 42 23 54 20 61 84 31 Total taxa 11 14 18 16 15 10 16 11 15 14 18 18 11 16 10 16 16 14 7 15 11 8 21 12 22 15 15 13 15 11 — = Species not collected at station 1-41 Table 1-34. Taxa collected from benthic ponar grab samples collected in May 2019 in all 15 creeks in the PCS study. Taxa for which EBI was calculated are shown with a * and other are shown with a V. Taxonomic hierarchy Broomfield Creek SCUT1 control Jacks Creek Little Creek (control) Jacobs Creek PA2 control Drinkwater Creek Long Creek control Tooley Creek Muddy Creek control Huddles Cut Porter Creek DCUT11 DCUT19 control Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Annelida Clitellata Enchytraeidae sp. Glossiphoniidae sp. Naididae Naididae sp. Naididae sp. w/ hair Dero sp. Naididae sp. w/o hair * * * Limnodrilus hoffineisteri Paranais litoralis Tubificoides heterochaetus Polychaeta Ampharetidae Amphicteis floridus * * * * * * * * * * * Capitellidae Heteromastus filiformis Mediomastus ambiseta Nereididae Neanthes succinea Spionidae Marenzelleria viridis Polydora cornuta Streblos io benedicti * * * * * Arthropoda Collembola Sminthuridae sp. Insecta Coleoptera Hydrophilidae Berosus sp. Haliplidae Peltodytes sp. Diptera Ceratopogonidae Bezzia/Palpomyia complex * * Chaoboridae Chaoborus albatus Chironomidae Apedilum sp. Chironomus decorus * * * * * * * * * * * * * * * * * * * 1-42 Table 1-34. (continued). Taxonomic hierarchy Broomfield Creek SCUT1 control Jacks Creek Little Creek (control) Jacobs Creek PA2 control Drinkwater Creek Long Creek control Tooley Creek Muddy Creek control Huddles Cut Porter Creek DCUT11 DCUT19 control Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Chironomus stigmaterus ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ Cladopelma sp. ✓ Clinotanypus sp. Coelotanypus sp. Cryptochironomus sp. Cryptotendipes sp. Dicrotendipes nervosus Dicrotendipes w/tubule ✓ Endochironomus sp. Goeldichironomus devineyae Goeldichironomus holoprasinus ✓ ✓ Glyptotendipes sp. Kiefferulus sp. ✓ ✓ ✓ ✓ ✓ Nanocladius sp. Orthocladiinae sp. ✓ Parachironomus sp. Polypedilum sp. Procladius sp. * * * Tanypus neopunctipennis * * * * * * * * * * * * * Tanytarsus limneticus ✓ ✓ ✓ ✓ Tanytarsus sp. * * * * * * * * * * * * Hemiptera Belostomatidae Abedus/Belostoma sp. Corixidae Corixidae sp. Trichocorixa calva ✓ Trichocorixa sexcinta Odonata Coenagrionidae Enallagma sp. Libellulidae Perithemis tenera ✓ ✓ ✓ ✓ ✓ Trichoptera Oecetis inconspicua Malacostraca Amphipoda Corophiidae Apocorophium lacustre Apocorophium louisianum Apocorophium sp. * * * * Gammaridae Gammarus tigrinus * * * * * * * * * * * * * * * * * Decapoda Palaemonidae Palaemonetes pugio Isopoda Anthuridae Cyathura polita Sphaeromatidae Cassidinidea lunifrons 1-43 Table 1-34. (concluded). Taxonomic hierarchy Broomfield Creek SCUT1 control Jacks Creek Little Creek (control) Jacobs Creek PA2 control Drinkwater Creek Long Creek control Tooley Creek Muddy Creek control Huddles Cut Porter Creek DCUT11 DCUT19 control Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Mysida Mysidae Americamysis almyra * * * * Ostracoda Candonidae Candonidae sp. ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ Cytherideidae Cyprideis littoralis ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓ Mollusca Bivalvia Bivalvia sp. ✓ ✓ Dreissenidae Mytilopsis leucophaeata Mactridae Mactridae sp. ✓ Rangia cuneata Tellinidae Macoma balthica Macoma tents Gastropoda Basommatophora Physidae sp. (physa /physella) Hydrobiidae Littoridinops sp. * * * * * * * * * * * * * * * * * * Total taxa 11 14 18 16 15 10 16 11 15 14 18 18 11 16 10 16 16 14 7 15 11 8 21 12 22 15 15 13 15 11 — = Species not collected at station 1-44 Table 1-35. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Broomfield Creek upstream station in May 2019. Species/taxon Broomfield Creek Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Dero sp. 3 3 0 0 12 3.6 Glossiphoniidae sp. 0 9 12 4 8 6.6 Limnoddlus hoffineistreri 12 0 12 16 16 11.2 Naididae sp. w/ hair 6 3 0 8 0 3.4 Naididae sp. w/o hair 0 6 21 80 56 32.6 Tubificoides heterochaetus 3 0 0 0 0 0.6 Arthropoda I nsecta D i pte ra Bezzia/Palpomyia complex 0 3 0 0 0 0.6 Chaoborus albatus 3 12 6 8 4 6.6 Chironomus decorus 0 0 3 0 0 0.6 Cryptochironomus sp. 0 0 0 4 0 0.8 Dicrotendipes nervosus 0 0 0 0 4 0.8 Parachironomus sp. 0 0 0 4 0 0.8 Mollusca Gastropoda Littoridinops sp. 6 0 0 0 0 1.2 Total taxa by sample 33 36 54 124 100 Total abundance = 347 Total taxa present = 11 Shannon -Wiener diversitv = 1.75 1-45 Table 1-36. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Broomfield Creek upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Dero sp. 18 1.0 10 10.0 Limnodrilus hoffineistreri 56 1.0 30 30.0 Naididae sp. w/ hair 17 1.0 10 10.0 Naididae sp. w/o hair 163 1.0 100 100.0 Tubificoides heterochaetus 3 1.1 3 3.3 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 3 2.3 3 6.9 Chironomus decorus 3 1.0 3 3.0 Cryptochironomus sp. 4 1.0 3 3.0 Dicrotendipes nervosus 4 1.0 3 3.0 Parachironomus sp. 4 1.1 3 3.3 Mollusca Gastropoda Littoridinops sp. 6 2.0 3 6.0 Total 171 178.5 Estuarine Biotic Index = 1.04 Total taxa = 11 Total taxa with sensitivitv values = 9 a Only those taxa for which a sensitivity value is available are displayed bTotal number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-46 Table 1-37. Numbers of benthic macro invertebrates collected in each ponar grab sample at the Broomfield Creek downstream station in May 2019. Species/taxon Broomfield Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Dero sp. 0 0 2 0 4 1.2 Limnodrilus hoffineisteri 24 29 11 4 10 15.6 Naididae sp. w/ hair 2 2 2 1 3 2.0 Naididae sp. w/o hair 56 42 56 21 42 43.4 Paranais litoralis 0 2 2 0 0 0.8 A rth ropoda I nsecta Diptera Chironomus decorus 2 4 9 2 4 4.2 Cladopelma sp. 0 0 0 1 0 0.2 Cryptochironomus sp. 0 0 4 0 0 0.8 Dicrotendipes nervosus 0 0 1 0 0 0.2 Endochironomus sp. 0 0 0 1 0 0.2 Procladius sp. 0 2 2 1 0 1.0 Hemiptera Trichocorixa calva 0 0 0 1 0 0.2 Odonata Perethemis tenera 1 0 0 0 0 0.2 Malacostraca Amphipoda Gammarus tigrinus 0 1 0 0 1 0.4 Mysida Americamysis almyra 0 0 2 0 0 0.4 Ostracoda Candonidae sp. 1 1 0 0 0 0.4 Total taxa by sample 86 83 91 32 64 Total abundance = 356 Total taxa present = 14 Shannon -Wiener diversity = 1.30 147 Table 1-38. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Broomfield Creek downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Dero sp. 6 1.0 3 3.0 Limnodrilus hoffineisteri 78 1.0 30 30.0 Naididae sp. w/ hair 10 1.0 10 10.0 Naididae sp. w/o hair 217 1.0 100 100.0 Paranais litoralis 4 1.1 3 3.3 Arthropoda Insecta Diptera Chironomus decorus 21 1.0 10 10.0 Cryptochironomus sp. 4 1.0 3 3.0 Dicrotendipes nervosus 1 1.0 1 1.0 Endochironomus sp. 1 1.1 1 1.1 Procladius sp. 5 1.2 3 3.6 Malacostraca Amphipoda Gammarus tigrinus 2 2.0 1 2.0 Mysida Americamysis almyra 2 2.2 1 2.2 Total 166 169.2 Estuarine Biotic Index = 1.02 Total taxa = 14 Total taxa with sensitivitv values = 10 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. 1-48 Table 1-39. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the SCUT1 upstream station in May 2019. Species/taxon SCUT1 Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Glossiphoniidae sp. 0 0 0 0 1 0.2 Limnoddlus hoffineisted 2 5 16 6 5 6.8 Naididae sp. w/o hair 43 47 100 53 40 56.6 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 3 3 6 5 0 3.4 Chironomus decorus 2 1 4 2 2 2.2 Clinotanypus sp. 0 1 1 0 1 0.6 Coelotanypus sp. 3 7 14 7 6 7.4 Cryptochironomus sp. 1 5 7 9 5 5.4 Dicrotendipes nervosus 2 0 0 1 0 0.6 Kiefferulus sp. 4 1 1 0 1 1.4 Polypedilum sp. 0 0 3 0 0 0.6 Procladius sp. 1 1 2 2 0 1.2 Tanypus neopunctipennis 2 10 16 16 4 9.6 Tanytarsus sp. 0 0 1 1 1 0.6 Odanata Enallagma sp. 1 0 0 0 0 0.2 Malacostraca Amphipoda Gammarus tigrinus 0 0 0 0 1 0.2 Ostracoda Cyprideis littoralis 1 0 0 1 0 0.4 Mollusca Bivalvia Bivalvia sp. 0 2 2 1 0 1.0 Gastropoda Littoridinops sp. 10 7 0 0 0 3.4 Total taxa by sample 1 75 1 90 173 1 104 67 Total abundance = 509 Total taxa present = 18 Shannon -Wiener diversity = 1.72 Mpe Table 1-40. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the SCUT1 upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Limnodrilus hoffineisteri 34 1.0 30 30.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 17 2.3 10 23.0 Chironomus decorus 11 1.0 10 10.0 Coelotanypus sp. 37 1.8 30 54.0 Cryptochironomus sp. 27 1.0 10 10.0 Dicrotendipes nervosus 3 1.0 3 3.0 Procladius sp. 6 1.2 3 3.6 Tanypus neopunctipennis 48 1.0 30 30.0 Tanytarsus sp. 3 2.3 3 6.9 Odanata Enallagma sp. 1 1.5 1 1.5 Malacostraca Amphipoda Gammarus tigrinus 1 2.0 1 2.0 Mollusca Gastropoda Littoridinops sp. 17 2.0 10 20.0 Total 141 194.0 Estuarine Biotic Index = 1.23 Total taxa present= 18 Total taxa with sensitivity values = 14 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-50 Table 1-41. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the SCUT1 downstream station in May 2019. Species/taxon SCUT1 Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Limnodrilus hoffineistreri 0 0 0 0 2 0.4 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 0 0 0 0 2 0.4 Chaoborus albatus 6 0 0 0 0 1.2 Chironomus decorus 12 0 14 4 8 7.6 Goeldichironomus devineyae 3 0 0 0 0 0.6 Kiefferulus sp. 0 3 0 0 0 0.6 Parachironomus sp. 0 0 2 0 0 0.4 Procladius sp. 24 0 12 14 12 12.4 Tanypus neopunctipennis 3 0 2 0 2 1.4 Tanytarsus sp. 3 0 0 0 4 1.4 Odonata Perithemis tenera 0 0 0 0 2 0.4 Malacostraca Amphipoda Gammarus tigrinus 0 0 0 0 8 1.6 Ostracoda Candonidae sp. 15 15 0 6 8 8.8 Cyprideis littoralis 6 0 0 0 16 4.4 Mollusca Bivalvia Rangia cuneata 6 0 0 0 0 1.2 Gastropoda Littoridinops sp. 3 0 0 0 2 1.0 Total taxa by sample 1 81 1 18 30 1 24 66 Total abundance = 219 Total taxa present = 16 Shannon -Wiener diversity = 2.13 1-51 Table 1-42. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the SCUT1 downstream station in May 2019. Species/taxona Number of individuals" Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Oligochaeta Limnodrilus hoffineisteri 2 1.0 1 1.0 Tubificida Naididae (Tubificinae) sp. w/o hair 283 1.0 100 100.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 2 2.3 1 2.3 Chironomus decorus 38 1.0 30 30.0 Goeldichironomus devineyae 3 2.0 3 6.0 Parachironomus sp. 2 1.1 1 1.1 Procladius sp. 62 1.2 30 36.0 Tanypus neopunctipennis 7 1.0 3 3.0 Tanytarsus sp. 7 2.3 3 6.9 Odanata Perithemis tenera 2 1.0 1 1.0 Malacostraca Amphipoda Gammarus tigrinus 8 2.0 3 6.0 Mollusca Bivalvia Rangia cuneata . 6 1.0 3 3.0 Gastropoda Littoridinops sp. 5 2.0 3 6.0 Total 81 101.3 Estuarine Biotic Index = 1.25 Total taxa = 16 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-52 Table 1-43. Numbers of benthic macro invertebrates collected in each ponar grab sample at the Jacks Creek upstream station in May 2019. Species/taxon Jacks Creek Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Dero sp. 0 1 0 0 0 0.2 Polychaeta Amphicteis floridus 3 2 4 6 0 3.0 Streblospio benedicti 2 0 0 2 0 0.8 Arthropoda Insecta Diptera Chironomus decorus 7 3 14 20 0 8.8 Glyptotendipes sp. 0 1 0 0 0 0.2 Parachironomus sp. 0 1 0 0 0 0.2 Tanypus neopunctipennis 4 4 2 0 4 2.8 Tanytarusu limneticus 1 2 2 0 0 1.0 Tanytarsus sp. 1 2 1 4 0 1.6 Hemiptera Corixidae sp. 1 1 0 0 0 0.4 Malacostraca Amphipoda Apocorophium louisianum 1 0 0 0 0 0.2 Apocorophium sp. 0 0 1 0 0 0.2 Gammarus tigrinus 2 5 3 0 0 2.0 Mys ida Americamysis almyra 7 0 0 2 0 1.8 Ostracoda Candonidae sp. 2 0 1 0 0 0.6 Cyprideis littoralis 1 0 0 0 0 0.2 Mollusca Gastropoda Littoridinops sp. 3 0 0 0 0 0.6 Total taxa by sample 35 22 1 28 34 1 4 Total abundance = 123 Total taxa present = 15 Shannon -Wiener diversity = 2.17 1-53 Table 1-44. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Jacks Creek upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Oligochaeta Dero sp. 1 1.0 1 1.0 Polychaeta Amphicteis floridus 15 2.0 10 20.0 Streblospio benedicti 4 1.1 3 3.3 Arthropoda I nsecta Diptera Chironomus decorus 44 1.0 30 30.0 Glyptotendipes sp. 1 1.3 1 1.3 Parachironomus sp. 1 1.1 1 1.1 Tanypus neopunctipennis 14 1.0 10 10.0 Tanytarsus sp. 8 2.3 3 6.9 Corixidae sp. 2 1.3 1 1.3 Malacostraca Amphipoda Apocorophium louisianum 1 2.2 1 2.2 Apocorophium sp. 1 2.2 1 2.2 Gammarus tigrinus 10 2.0 10 20.0 Mysida Americamysis almyra 9 2.2 3 6.6 Mollusca Gastropoda Littoridinops sp. 3 2.0 3 6.0 Total 78 111.9 Estuarine Biotic Index = 1.43 Total taxa = 15 Total taxa with sensitivity values = 12 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. 'Sensitivity values provided by NCDWR. 1-54 Table 1-45. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Jacks Creek downstream station in May 2019. Species/taxon Jacks Creek Downstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 0 0 3 4 10 3.4 Streblospio benedicti 0 0 1 1 0 0.4 Arthropoda I nsecta Diptera Chironomus decorus 26 25 23 28 46 29.6 Tanytarsus sp. 1 0 1 1 2 1.0 Malacostraca Amphipoda Gammarus tigrinus 0 1 4 0 0 1.0 Mysida Americamysis almyra 0 0 0 0 1 0.2 Ostracoda Candonidae sp. 0 1 0 2 0 0.6 Cyprideis littoralis 2 1 0 0 3 1.2 Mollusca Bivalvia Rangia cuneata 0 0 0 1 0 0.2 Gastropoda Littoddinops sp. 2 1 1 0 0 0.8 Total taxa by sample 31 29 33 37 62 Total abundance = 192 Total taxa present = 10 Shannon -Wiener diversity = 0.96 1-55 Table 1-46. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Jacks Creek downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value n Product of SV n Annelida Polychaeta Amphicteis floridus 17 2.0 10 20.0 Streblospio benedicti 2 1.1 1 1.1 Arthropoda Crustacea Americamysis almyra 1 2.2 1 2.2 I nsecta Diptera Chironomus decorus 148 1.0 100 100.0 Tanytarsus sp. 5 2.3 3 6.9 Malacostraca Amphipoda Gammarus tigrinus 5 2.0 3 6.0 Mollusca Bivalvia Rangia cuneata 1 1.0 1 1.0 Gastropoda Littoridinops sp. 4 2.0 3 6.0 Total 118 136.2 Estuarine Biotic Index = 1.17 Total taxa = 10 Total taxa with sensitivitv values = 8 a Only those taxa for which a sensitivity value is available are displayed bTotal number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-56 Table 1-47. Numbers of benthic macroi nve rte b rates collected in each ponar grab sample at the Little Creek upstream station in May 2019. Species/taxon Little Creek Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 12 3 42 54 36 29.4 Arthropoda I nsecta D i pte ra Bezzia/Palpomyia complex 0 0 3 0 0 0.6 Chironomus decorus 12 6 36 30 6 18.0 Dicrotendipes nervosus 3 3 3 3 0 2.4 Dicrotendipes w/ tubule 0 0 0 0 6 1.2 Glyptotendipes sp. 3 0 0 6 0 1.8 Goeldichironomus devineyae 3 0 0 0 0 0.6 Kiefferulus sp. 60 0 3 15 18 19.2 Polypedilum sp. 3 0 0 0 0 0.6 Procladius sp. 12 3 12 6 0 6.6 Tanypus neopuntipennis 3 0 0 0 0 0.6 Tanytarsus limneticus 3 0 0 0 0 0.6 Tanytarsus sp. 12 6 3 6 9 7.2 Malacostraca Amphipoda Gammarus tigrinus 81 33 21 69 51 51.0 Mollusca Bivalvia Mytilopsis leucophaeata 0 3 0 0 0 0.6 Gastropoda Littoridinops sp. 0 6 0 0 0 1.2 Physidae sp. (physalphysella) 9 6 3 3 3 4.8 Total taxa by sample 216 69 126 192 129 Total abundance = 732 Total taxa present = 16 Shannon -Wiener diversitv = 1.95 1-57 Table 1-48. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Little Creek upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 147 1.0 100 100.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 3 2.3 3 6.9 Chironomus decorus 90 1.0 30 30.0 Dicrotendipes nervosus 12 1.0 10 10.0 Glyptotendipes sp. 9 1.0 3 3.0 Goeldichironomus devineyae 3 2.0 3 6.0 Procladius sp. 33 1.2 30 36.0 Tanypus neopuntipennis 3 1.0 3 3.0 Tanytarsus sp. 36 2.3 30 69.0 Malacostraca Amphipoda Gammarus tigrinus 255 2.0 100 200.0 Mollusca Bivalvia Mytilopsis leucophaeata 3 1.5 3 4.5 Gastropoda Littoridinops sp. 6 2.0 3 6.0 Physidae sp. 24 1.5 10 15.0 Total 318 474.4 Estuarine Biotic Index = 1.49 Total taxa = 16 Total taxa with sensitivity values = 13 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-58 Table 1-49. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Little Creek downstream station in Mav of 2019. Species/taxon Little Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Dero sp. 0 0 0 0 3 0.6 Polychaeta Amphicteis floridus 1 1 1 0 1 0.8 Streblospio benedicti 2 5 32 1 22 12.4 Arthropoda Insecta Diptera Chironomus decorus 100 93 92 100 100 97.0 Chironomus stigmaterus 1 0 0 0 0 0.2 Tanypus neopunctipennis 1 0 1 1 0 0.6 Hemiptera Corixidae sp. 0 0 0 0 1 0.2 Trichocorixa sexcinta 0 1 0 0 0 0.2 Malacostraca Amphipoda Apocorophium sp. 0 0 1 0 0 0.2 Gammarus tigrinus 1 1 1 2 0 1.0 Mysida Americamysis almyra 0 0 0 1 0 0.2 Mollusca Gastropoda Littoridinops sp. 23 2 8 0 1 6.8 Total taxa by sample 129 103 136 105 128 Total abundance = 601 Total taxa present = 11 Shannon -Wiener diversity = 0.75 1-59 Table 1-50. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Little Creek downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 4 2.0 3 6.0 Streblospio benedicti 62 1.1 30 33.0 Oligocheata Dero sp. 3 2.0 3 6.0 Arthropoda Insecta Diptera Chironomus decorus 485 1.0 100 100.0 Tanypus neopunctipennis 3 1.0 3 3.0 Hemiptera Corixidae sp. 1 1.3 1 1.3 Trichocorixa sexcincta 1 1.0 1 1.0 Malacostraca Amphipoda Apocorophium sp. 1 2.2 1 2.2 Gammarus tigrinus 5 2.0 3 6.0 Mysida Americamysis almyra 1 2.2 1 2.2 Mollusca Gastropoda Littoridinops sp. 34 2.0 30 60.0 Total 176 220.7 Estuarine Biotic Index = 1.24 Total taxa = 11 Total taxa with sensitivitv values = 10 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. Table 1-51. Numbers of benthic macro i nve rte brates collected in each ponar grab sample at the Jacobs Creek upstream station in May 2019. Species/taxon Jacobs Creek Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 0 0 0 2 0 0.4 Polychaeta Amphicteis floridus 0 0 8 28 5 8.2 Streblospio benedicti 0 0 2 0 2 0.8 Arthropoda I nsecta Diptera Chironomus decorus 31 14 56 26 20 29.4 Chironomus stigmaterus 0 0 0 2 0 0.4 Endochironomus sp. 0 0 0 0 1 0.2 Parachironomus sp. 0 0 0 10 1 2.2 Tanypus neopunctipennis 8 12 2 10 4 7.2 Tanytarusu limneticus 0 0 6 4 0 2.0 Tanytarsus sp. 6 10 4 10 5 7.0 Malacostraca Amphipoda Apocorophium sp. 0 0 0 2 1 0.6 Gammarus tigrinus 1 2 0 24 1 5.6 Mysida Americamysis almyra 0 0 0 0 1 0.2 Ostracoda Candonidae sp. 3 2 4 4 0 2.6 Cyprideis littoralis 1 0 0 2 1 0.8 Mollusca Gastropoda Littoridinops sp. 1 0 0 0 2 0.6 Total taxa by sample 51 40 82 124 44 Total abundance = 341 Total taxa present = 15 Shannon -Wiener diversity = 1.92 1-61 Table 1-52. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Jacobs Creek upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 2 1.0 1 1.0 Polychaeta Amphicteis floridus 41 2.0 30 60.0 Streblospio benedicti 4 1.1 3 3.3 Arthropoda I nsecta Diptera Chironomus decorus 147 1.0 100 100.0 Endochironomus sp. 1 1.1 1 1.1 Parachironomus sp. 11 1.1 10 11.0 Tanypus neopunctipennis 36 2.3 30 69.0 Tanytarsus sp. 35 2.3 30 69.0 Mysida Americamysis almyra 1 2.2 1 2.2 Malacostraca Amphipoda Apocorophium sp. 3 2.2 3 6.6 Gammarus tigrinus 28 2.0 10 20.0 Mollusca Gastropoda Littoridinops sp. 3 2.0 3 6.0 Total 222 349.2 Estuarine Biotic Index = 1.40 Total taxa = 15 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed bTotal number of individuals caught in all five ponar replicates. 'Sensitivity values provided by NCDWR. 1-62 Table 1-53. Numbers of benthic macro i nve rte brates collected in each ponar grab sample at the Jacobs Creek downstream station in May 2019. Species/taxon Jacobs Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Tubificoides heterochaetus 2 0 1 0 2 1.0 Polychaeta Amphicteis floridus 2 1 0 1 0 0.8 Streblospio benedicti 11 3 8 2 13 7.4 Arthropoda I nsecta Diptera Bezzia/Palpomyia complex 0 0 1 0 0 0.2 Chironomus decorus 100 100 100 91 100 98.2 Chironomus stigmaterus 2 0 1 0 2 1.0 Tanypus neopunctipennis 0 0 0 1 1 0.4 Tanytarsus sp. 0 0 2 0 2 0.8 Hemiptera Corixidae sp. 0 0 1 0 0 0.2 Malacostraca Mysida Americamysis almyra 1 2 1 0 10 2.8 Ostracoda Candonidae sp. 0 0 0 1 0 0.2 Cyprideis littoralis 7 2 2 0 3 2.8 Mollusca Bivalvia Macoma tenta 0 0 1 0 1 0.4 Gastropoda Littoddinops sp. 0 0 31 0 4 7.0 Total taxa by sample 125 108 149 96 138 Total abundance = 616 Total taxa present = 14 Shannon -Wiener diversitv = 0.90 1-63 Table 1-54. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Jacobs Creek downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Tubificoides heterochaetus 3 1.1 3 3.3 Polychaeta Amphicteis floridus 4 2.0 3 6.0 Streblospio benedicti 37 1.1 30 33.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 1 2.3 1 2.3 Chironomus decorus 491 1.0 100 100.0 Tanypus neopunctipennis 2 2.3 1 2.3 Tanytarsus sp. 4 2.3 3 6.9 Hemiptera Corixidae sp. 1 1.3 1 1.3 Mysida Americamysis almyra 14 2.2 10 22.0 Mollusca Bivalvia Macoma tenta 2 2.0 1 2.0 Gastropoda Littoridinops sp. 35 2.0 30 60.0 Total 183 239.1 Estuarine Biotic Index = 1.30 Total taxa = 14 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed bTotal number of individuals caught in all five ponar replicates. 'Sensitivity values provided by NCDWR. 1-64 Table 1-55. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the PA2 upstream station in May 2019. Species/taxon PA2 Creek Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 1 0 0 1 1 0.6 Polychaeta Amphicteis floridus 6 1 2 4 5 3.6 Streblospio benedicti 5 1 4 1 11 4.4 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 0 0 0 1 0 0.2 Chironomus decorus 83 93 48 71 68 72.6 Chironomus stigmaterus 2 1 1 3 0 1.4 Tanypus neopunctipennis 3 2 16 5 4 6.0 Tanytarusu limneticus 2 0 2 0 4 1.6 Tanytarsus sp. 32 2 15 1 8 11.6 Hemiptera Corixidae sp. 1 0 0 0 0 0.2 Odanata Enallagma sp. 1 0 0 0 0 0.2 Malacostraca Amphipoda Apocorophium louisianum 1 0 0 1 0 0.4 Apocorophium sp. 1 0 0 3 0 0.8 Gammarus tigrinus 1 1 3 1 0 1.2 Decapoda Palaemonetes pugio 0 1 0 0 0 0.2 Isopoda Cassidinidea lunifrons 1 0 0 0 0 0.2 Mysida Americamysis almyra 3 0 0 0 0 0.6 Ostracoda Candonidae sp. 0 0 0 0 2 0.4 Cyprideis littoralis 0 0 1 0 0 0.2 Mollusca Gastropoda Littoridinops sp. 9 1 41 5 1 11.4 Total taxa by sample 1 152 1 103 1 133 1 97 1 104 Total abundance = 589 Total taxa present = 18 Shannon -Wiener diversity = 1.48 Table 1-56. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the PA2 downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 3 1.0 3 3.0 Polychaeta Amphicteis floridus 18 2.0 10 20.0 Streblospio benedecti 22 1.1 10 11.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 1 2.3 1 2.3 Chironomus decorus 363 1.0 100 100.0 Tanypus neopunctipennis 30 1.0 30 30.0 Tanytarsus sp. 58 2.3 30 69.0 Hemiptera Corixidae sp. 1 1.3 1 1.3 Crustacea Mysidae Americamysis almya 2.2 0.0 Odanata Enallagma sp. 1 2.3 1 2.3 Malacostraca Amphipoda Apocorophium louisianum 2 2.2 1 2.2 Apocorophium sp. 4 2.2 3 6.6 Gammarus tigrinus 6 2.0 3 6.0 Decopoda Palaemonetes pugio 1 2.5 1 2.5 Isopoda Cassidinidea lunifrons 1 2.0 1 2.0 Mollusca Gastropoda Littoridinops sp. 57 2.0 30 60.0 Total 225 318.2 Estuarine Biotic Index = 1.42 Total taxa = 18 Total taxa with sensitivity values = 14 Table 1-57. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the PA2 downstream station in May 2019. Species/taxon PA2 Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 0 1 0 2 0 0.6 Tubificoides heterochaetus 0 0 0 1 0 0.2 Polychaeta Amphicteis floridus 4 10 1 7 22 8.8 Streblospio benedecti 15 2 7 7 4 7.0 Arthropoda Collembola Sminthuridae sp. 0 1 0 0 0 0.2 Insecta Diptera Bezzia/Palpomyia complex 0 1 0 0 0 0.2 Chironomus decorus 62 96 44 100 100 80.4 Chironomus stigmaterus 0 0 1 1 1 0.6 Parachironomus sp. 0 0 0 0 1 0.2 Procladius sp. 0 0 1 1 0 0.4 Tanypus neopunctipennis 1 7 10 12 13 8.6 Tanytarsus sp. 1 4 6 3 8 4.4 Odanata Enallagma sp. 0 1 0 0 0 0.2 Malacostraca Amphipoda Apocorophium lacustre 0 1 0 0 0 0.2 Apocorophium louisianum 0 0 0 0 1 0.2 Apocorophium sp. 1 1 0 0 1 0.6 Gammarus tigrinus 1 1 1 0 8 2.2 Mys ida Americamysis almyra 0 0 0 2 0 0.4 Ostracoda Cyprideis littoralis 0 2 0 0 0 0.4 Mollusca Gastropoda Littoridinops sp. 20 59 2 3 3 17.4 Total taxa by sample 105 187 1 73 139 1 162 Total abundance = 666 Total taxa present = 18 Shannon -Wiener diversity = 1.46 1-67 Table 1-58. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the PA2 downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 3 1.0 3 3.0 Tubificoides heterochaetus 1 1.1 1 1.1 Polychaeta Amphicteis floridus 44 2.0 30 60.0 Streblospio benedecti 35 1.1 30 33.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 1 2.3 1 2.3 Chironomus decorus 402 1.0 100 100.0 Parachironomus sp. 1 1.1 1 1.1 Procladius sp. 2 1.2 1 1.2 Tanypus neopunctipennis 43 1.0 30 30.0 Tanytarsus sp. 22 2.3 10 23.0 Crustacea Mysidae Americamysis almya 2 2.2 1 2.2 Odanata Enallagma sp. 1 2.3 1 2.3 Malacostraca Amphipoda Apocorophium lacustre 1 2.2 1 2.2 Apocorophium louisianum 1 2.2 1 2.2 Apocorophium sp. 3 2.2 3 6.6 Gammarus tigrinus 11 2.0 10 20.0 Mollusca Gastropoda Littoridinops sp. 87 2.0 30 60.0 Total 254 350.2 Estuarine Biotic Index = 1.38 Total taxa = 18 Total taxa with sensitivity values = 15 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. .: Table 1-59. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Drinkwater Creek upstream station in May 2019. Species/taxon Drinkwater Creek Upstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 12 36 21 12 6 17.4 Arthropoda I nsecta D i pte ra Chironomus decorus 12 12 9 0 0 6.6 Chironomus stigmaterus 18 45 54 24 8 29.8 Tanypus neopunctipennis 0 0 6 0 0 1.2 Tanytarsus sp. 24 21 9 9 18 16.2 Hemiptera Corixidae sp. 0 0 3 0 0 0.6 Trichocorixa sexcinta 0 3 9 0 0 2.4 Malacostraca Amphipoda Apocorophium sp. 0 3 3 0 0 1.2 Gammarus tigrinus 12 6 15 0 2 7.0 Ostracoda Candonidae sp. 21 15 12 42 2 18.4 Mollusca Bivalvia Veneroida Mytilopsis leucophaeata 0 0 0 0 2 0.4 Gastropoda Littoridinops sp. 12 6 6 0 0 4.8 Total taxa by sample 111 147 147 87 38 Total abundance = 530 Total taxa present = 11 Shannon -Wiener diversity = 1.97 Table 1-60. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Drinkwater Creek upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 87 2.0 30 60.0 Arthropoda Insecta Diptera Chironomus decorus 33 1.0 30 30.0 Tanypus neopunctipennis 6 1.0 3 3.0 Tanytarsus sp. 81 2.3 30 69.0 Hemiptera Corixidae sp. 3 1.3 3 3.9 Trichocorixa sexcinta 12 1.0 10 10.0 Malacostraca Amphipoda Apocorophium sp. 6 2.2 3 6.6 Gammarus tigrinus 35 2.0 30 60.0 Mollusca Bivalvia Mytilopsis leucophaeata 2 2.0 1 2.0 Gastropoda Littoridinops sp. 24 2.0 10 20.0 Total 150 264.5 Estuarine Biotic Index = 1.76 Total taxa = 11 Total taxa with sensitivity values = 9 a Only those taxa for which a sensitivity value is available are displayed bTotal number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-70 Table 1-61. Numbers of benthic macro invertebrates collected in each ponar grab sample at the Drinkwater Creek downstream station in May 2019. Species/taxon Drinkwater Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Tubificoides heterochaetus 0 0 0 1 0 0.2 Polychaeta Amphicteis floridus 2 7 1 4 7 4.2 Streblospio benedicti 25 29 4 38 14 22.0 A rth ropoda I nsecta Diptera Chironomus decorus 100 100 86 100 100 97.2 Chironomus stigmaterus 4 2 1 6 2 3.0 Tanypus neopunctipennis 0 2 2 0 0 0.8 Tanytarsus sp. 5 0 2 1 1 1.8 Hemiptera Trichocorixa sexcinta 0 1 0 0 0 0.2 Malacostraca Amphipoda Apocorophium sp. 1 0 0 0 0 0.2 Gammarus tigrinus 2 1 3 1 0 1.4 Mysida Americamysis almyra 0 0 1 0 2 0.6 Ostracoda Candonidae sp. 0 0 0 1 0 0.2 Cyprideis littoralis 0 0 0 0 1 0.2 Mollusca Bivalvia Bivalvia sp. 0 1 0 0 0 0.2 Macoma tenta 0 2 0 0 0 0.4 Mactridae sp. 0 1 0 0 0 0.2 Gastropoda Littoridinops sp. 0 4 3 2 0 1.8 Total taxa by sample 139 150 103 154 127 Total abundance = 673 Total taxa present = 16 Shannon -Wiener diversitv = 1.03 1-71 Table 1-62. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Drinkwater Creek downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Tubificoides heterochaetus 1 1.1 1 1.1 Polychaeta Amphicteis floridus 21 2.0 10 20.0 Streblospio benedicti 110 1.1 100 110.0 Arthropoda Insecta Diptera Chironomus decorus 486 1.0 100 100.0 Tanypus neopunctipennis 4 1.0 3 3.0 Tanytarsus sp. 9 2.3 3 6.9 Hemiptera Trichocorixa sexcinta 1 1.0 1 1.0 Malacostraca Amphipoda Apocorophium sp. 1 2.2 1 2.2 Gammarus tigrinus 7 2.0 3 6.0 Mysida Americamysis almyra 3 2.2 3 6.6 Mollusca Bivalvia Macoma tenta 2 2.0 1 2.0 Gastropoda Littoridinops sp. 9 2.0 3 6.0 Total 229 264.8 Estuarine Biotic Index = 1.16 Total taxa = 16 Total taxa with sensitivity values = 12 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. 1-73 Table 1-63. Numbers of benthic macro invertebrates collected in each ponar grab sample at the Long Creek upstream station in Mav 2019. S ecies/taxon Long Creek Up stream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 0 0 0 1 0 0.2 Streblospio benedicti 3 4 2 3 0 2.4 Arthropoda Insecta Diptera Chironomus decorus 33 24 43 51 28 35.8 Tanypus neopunctipennis 0 0 1 1 0 0.4 Hemiptera Corixidae sp. 0 2 1 0 0 0.6 Malacostraca Amphipoda Apocorophium louisianum 1 0 0 0 0 0.2 Apocorophium sp. 3 2 4 0 0 1.8 Gammarus tigrinus 4 7 2 1 1 3.0 Mysida Americamysis almyra 2 0 1 0 1 0.8 Ostracoda Cyprideis littoralis 0 1 0 2 0 0.6 Mollusca Gastropoda Littoridinops sp. 6 8 1 1 0 3.2 Total taxa by sample 52 48 55 60 30 Total abundance = 245 Total taxa present = 10 Shannon -Wiener diversitv = 1.11 1-73 Table 1-64. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Long Creek upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)° Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 1 2.0 1 2.0 Streblospio benedicti 12 1.1 10 11.0 Arthropoda Insecta Diptera Chironomus decorus 179 1.0 100 100.0 Tanypus neopunctipennis 2 1.0 1 1.0 Hemiptera Corixidae sp. 3 1.3 3 3.9 Malacostraca Amphipoda Apocorophium louisianum 1 2.2 1 2.2 Apocorophium sp. 9 2.2 3 6.6 Gammarus tigrinus 15 2.0 10 20.0 Mysida Americamysis almyra 4 2.2 3 6.6 Mollusca Gastropoda Littoridinops sp. 16 2.0 10 20.0 Total 142 173.3 Estuarine Biotic Index = 1.22 Total taxa = 10 Total taxa with sensitivitv values = 9 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-74 Table 1-65. Numbers of benthic macro invertebrates collected in each ponar grab sample at the Long Creek downstream station in May 2019. Species/taxon Long Creek Downstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 1 0 0 0 0 0.2 Streblospio benedicti 9 3 7 10 2 6.2 Arthropoda Insecta Coleoptera Berosus sp. 0 1 0 0 0 0.2 Diptera Chironomus decorus 100 63 70 100 88 84.2 Dicrotendipes nervosus 1 0 0 0 0 0.2 Tanypus neopunctipennis 0 2 1 4 1 1.6 Hemiptera Corixidae sp. 4 0 2 1 1 1.6 Trichocorixa sexcinta 0 0 1 0 0 0.2 Odanata Enallagma sp. 3 0 1 1 0 1.0 Malacostraca Amphipoda Apocorophium sp. 0 1 0 0 1 0.4 Gammarus tigrinus 3 0 4 3 1 2.2 Isopoda Cassidinidea lunifrons 0 0 0 1 1 0.4 Mysida Americamysis almyra 0 0 0 0 1 0.2 Ostracoda Cyprideis littoralis 0 0 1 0 1 0.4 Mollusca Bivalvia Macoma tenta 1 1 2 0 1 1.0 Gastropoda Littoridinops sp. 15 0 6 6 4 6.2 Physidae sp. (physalphysella) 0 0 1 0 0 0.2 Total taxa by sample 1 137 1 71 1 96 1 126 1 102 Total abundance = 532 Total taxa present = 16 Shannon -Wiener diversity = 0.94 Table 1-66. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Long Creek downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value n Product of SV n Annelida Polychaeta Amphicteis floridus 1 2.0 1 2.0 Streblospio benedicti 31 1.1 30 33.0 Arthropoda Insecta Diptera Chironomus decorus 421 1.0 100 100.0 Dicrotendipes nervosus 1 1.0 1 1.0 Tanypus neopunctipennis 8 2.3 3 6.9 Coleoptera Berosus sp. 1 1.5 1 1.5 Hemiptera Corixidae sp. 8 1.3 3 3.9 Trichocorixa sexcincta 1 1.0 1 1.0 Odanata Enallagma sp. 5 1.5 3 4.5 Malacostraca Amphipoda Apocorophium sp. 2 2.2 1 2.2 Gammarus tigrinus 11 2.0 10 20.0 Isopoda Cassidinidea lunifrons 2 2.0 1 2.0 Mysida Americamysis almyra 1 2.2 1 2.2 Mollusca Bivalvia Macoma tenta 5 2.0 3 6.0 Gastropoda Littoridinops sp. 31 2.0 30 60.0 Physidae sp. 1 1.5 1 1.5 Total 190 247.7 Estuarine Biotic Index = 1.28 Total taxa = 16 Total taxa with sensitivitv values = 15 a Only those taxa for which a Sensitivity value is available are displayed bTotal number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-76 Table 1-67. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Tooley Creek upstream station in May 2019. Species/taxon Tooley Creek Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 0 2 0 0 0 0.4 Tubificoides heterochaetus 2 0 1 0 0 0.6 Polychaeta Amphicteis floridus 16 16 10 0 0 8.4 Streblospio benedicti 12 0 5 1 0 3.6 Arthropoda Insecta Diptera Apedilum sp. 2 0 0 0 0 0.4 Bezzia/Palmonyia complex 0 0 2 0 0 0.4 Chironomus decorus 18 4 51 34 29 27.2 Goeldichironomus devinyae 0 0 1 0 0 0.2 Parachironomus sp. 2 0 0 0 0 0.4 Tanypus neopunctipennis 4 2 1 1 1 1.8 Tanytarsus sp. 2 18 2 2 2 5.2 Odanata Enallagma sp. 0 0 1 0 0 0.2 Malacostraca Amphipoda Apocorophium louisianum 0 8 0 0 0 1.6 Apocorophium sp. 2 22 5 2 2 6.6 Gammarus tigrinus 10 0 5 1 2 3.6 Mysida Americamysis almyra 0 0 2 0 0 0.4 Ostracoda Cyprideis littoralis 20 2 8 0 4 6.8 Mollusca Gastropoda Littoridinops sp. 10 2 2 2 5 4.2 Total taxa by sample 100 76 96 43 45 Total abundance = 360 Total taxa present = 16 Shannon -Wiener diversity = 2.11 drl Table 1-68. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Tooley Creek upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 2 1.0 1 1.0 Oligochaeta Tubificoides heterochaetus 3 1.1 3 3.3 Polychaeta Streblospio benedicti 18 1.1 10 11.0 Arthropoda I nsecta Diptera Apedilum sp. 2 4.0 1 4.0 Bezzia/Palmonyia complex 2 2.3 1 2.3 Chironomus decorus 136 1.0 100 100.0 Goeldichironomus devineyae 1 2.0 1 2.0 Parachironomus sp. 2 1.1 1 1.1 Tanypus neopunctipennis 9 1.0 3 3.0 Tanytarsus sp. 26 2.3 10 23.0 Odanata Enallagma sp. 1 1.5 1 1.5 Malacostraca Amphipoda Apocorophium louisianum 8 2.2 3 6.6 Apocorophium sp. 33 2.2 30 66.0 Gammarus tigrinus 18 2.0 10 20.0 Tanaidacea Mysida Americamysis almyra 2 2.2 1 2.2 Mollusca Gastropoda Littoridinops sp. 21 2.0 10 20.0 Polychaeta Terebellida Amphicteis floridus 42 1.5 30 45.0 Total I 1 186 1 267.0 Estuarine Biotic Index = 1.51 Total taxa = 16 Total taxa with sensitivity values = 15 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. I dE:3 Table 1-69. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Tooley Creek downstream station in May 2019. Species/taxon Tooley Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Tubificoides heterochaetus 5 1 4 0 1 2.2 Polychaeta Mediomastus ambiseta 3 2 5 0 1 2.2 Polydora cornuta 0 0 1 0 0 0.2 Streblospio benedicti 22 4 19 2 1 9.6 Arthropoda I nsecta Diptera Chironomus decorus 88 36 52 33 55 52.8 Glyptotendipes sp. 1 0 0 0 0 0.2 Tanypus neopunctipennis 0 0 2 0 0 0.4 Odanata Enallagma sp. 1 0 0 0 0 0.2 Malacostraca Amphipoda Gammarus tigrinus 1 3 2 0 0 1.2 Isopoda Cassidinidea lunifrons 1 0 1 0 0 0.4 Mysida Americamysis almyra 0 0 2 1 0 0.6 Ostracoda Cyprideis littoralis 0 1 0 0 0 0.2 Mollusca Bivalvia Macoma tenta 1 0 0 0 0 0.2 Gastropoda Littoridinops sp. 1 0 1 1 0 0.6 Total taxa by sample 124 47 89 37 58 Total abundance = 355 Total taxa present = 14 Shannon -Wiener diversitv = 1.00 1-79 Table 1-70. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Tooley Creek downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Tubificoides heterochaetus 11 1.1 10 11.0 Polychaeta Mediomastus ambiseta 11 2.0 10 20.0 Polydora cornuta 1 1.2 1 1.2 Streblospio benedicti 48 1.1 30 33.0 Arthropoda I nsecta Diptera Chironomus decorus 264 1.0 100 100.0 Glyptotendipes sp. 1 1.3 1 1.3 Tanypus neopunctipennis 2 1.0 1 1.0 Odanata Enallagma sp. 1 1.5 1 1.5 Malacostraca Amphipoda Gammarus tigrinus 6 2.0 3 6.0 Isopoda Cassidinidea lunifrons 2 2.0 1 2.0 Mysida Americamysis almyra 3 2.2 3 6.6 Mollusca Bivalvia Macoma tenta 1 2.0 1 2.0 Gastropoda Littoridinops sp. 3 2.0 3 6.0 Total 165 191.6 Estuarine Biotic Index = 1.16 Total taxa = 14 Total taxa with sensitivity values = 13 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. Table 1-71. Numbers of benthic macro invertebrates collected in each ponar grab sample at the Muddy Creek upstream station in May 2019. Species/taxon Muddy Creek Upstream Samples 1 2 3 4 5 Average Arthropoda Insecta Diptera Chironomus decorus 36 5 7 28 10 17.2 Procladius sp. 1 0 0 0 0 0.2 Tanypus neopunctipennis 3 1 0 0 0 0.8 Odanata Enallagma sp. 0 0 1 0 0 0.2 Malacostraca Amphipoda Gammarus tigrinus 1 0 0 0 0 0.2 Ostracoda Candonidae sp. 38 26 2 24 35 25.0 Mollusca Gastropoda Littoridinops sp. 1 0 0 1 0 0.4 Total taxa by sample 80 32 1 10 53 1 45 Total abundance = 220 Total taxa present = 7 Shannon -Wiener diversity = 0.88 1-81 Table 1-72. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Muddy Creek upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Arthropoda Insecta Diptera Chironomus decorus 86 1.0 30 30.0 Procladius sp. 1 1.2 1 1.2 Tanypus neopunctipennis 4 1.0 3 3.0 Odanata Enallagma sp. 1 1.0 1 1.0 Malacostraca Amphipoda Gammarus tigrinus 1 2.0 1 2.0 Mollusca Gastropoda Littoridinops sp. 2 2.0 1 2.0 Total 37 39.2 Estuarine Biotic Index = 1.07 Total taxa = 7 Total taxa with sensitivity values = 6 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-82 Table 1-73. Numbers of benthic macro i nve rte brates collected in each ponar grab sample at the Muddy Creek downstream station in May 2019. Species/taxon Muddy Creek Downstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 1 1 1 1 0 0.8 Streblospio benedicti 15 5 0 0 2 4.4 Arthropoda I nsecta Diptera Chironomus decorus 100 76 53 28 99 71.2 Tanytarsus limneticus 10 0 0 0 0 2.0 Tanytarsus sp. 0 0 1 0 0 0.2 Hemiptera Corixidae sp. 0 3 11 3 0 3.4 Trichocorixa sexcinta 0 2 0 0 0 0.4 Odanata Enallagma sp. 0 1 1 0 0 0.4 Malacostraca Amphipoda Apocorophium sp. 1 0 0 0 9 2.0 Gammarus tigrinus 0 3 1 0 0 0.8 Isopoda Cassidinidea lunifrons 0 1 1 0 0 0.4 Mysida Americamysis almyra 2 0 2 1 1 1.2 Ostracoda Candonidae sp. 0 0 0 0 1 0.2 Cyprideis littoralis 0 0 0 0 1 0.2 Mollusca Gastropoda Littoridinops sp. 0 9 9 0 0 3.6 Physidae sp. (physalphysella) 0 0 1 0 0 0.2 Total taxa by sample 129 101 81 33 113 Total abundance = 457 Total taxa present = 15 Shannon -Wiener diversity = 1.02 1-83 Table 1-74. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Muddy Creek downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 4 2.0 3 6.0 Streblospio benedicti 22 1.1 10 11.0 Arthropoda Insecta Diptera Chironomus decorus 356 1.0 100 100.0 Tanypus neopunctipennis 10 1.0 10 10.0 Tanytarsus sp. 1 2.3 10 23.0 Hemiptera Corixidae sp. 17 1.3 10 13.0 Trichocorixa sexcincta 2 1.0 1 1.0 Odanata Enallagma sp. 2 1.0 1 1.0 Malacostraca Amphipoda Apocorophium sp. 10 2.2 10 22.0 Gammarus tigrinus 4 2.0 3 6.0 Isopoda Cassidinidea lunifrons 2 2.0 1 2.0 Mysida Mysidae Americamysis almyra 6 2.2 3 6.6 Mollusca Littoridinops sp. 18 2.0 10 20.0 Physidae sp. 2 2.0 1 2.0 Total 1 172 221.6 Estuarine Biotic Index = 1.24 Total taxa = 15 Total taxa with sensitivitv values = 13 a Only those taxa for which a sensitivity value is available are displayed bTotal number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-84 Table 1-75. Numbers of benthic macroi nve rteb rates collected in each ponar grab sample at the Huddles Cut upstream station in May 2019. Species/taxon Huddles Cut Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Enchytraeidae sp. 0 0 0 0 2 0.4 Polychaeta Amphicteis floridus 0 0 1 0 0 0.2 Neanthes succinea 2 0 3 0 0 1.0 Arthropoda I nsecta Di pte ra Chironomus decorus 66 99 38 96 80 75.8 Chironomus stigmaterus 0 0 1 0 0 0.2 Goeldichironomus devineyae 8 2 0 0 0 2.0 Tanypus neopunctipennis 18 16 3 18 2 11.4 Tanytarsus sp. 0 0 0 1 2 0.6 Ostracoda Cyprideis littoralis 4 2 8 10 14 7.6 Mollusca Bivalvia Rangia cuneata 0 0 1 1 0 0.4 Gastropoda Littoridinops sp. 2 27 1 5 4 7.8 Total taxa by sample 100 146 56 131 104 Total abundance = 537 Total taxa present = 11 Shannon -Wiener diversity = 1.07 1-85 Table 1-76. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Huddles Cut upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 2 1.0 1 1.0 Polychaeta Amphicteis floridus 1 2.0 1 2.0 Neanthes succinea 5 1.7 3 5.1 Arthropoda Insecta Diptera Chironomus decorus 379 1.0 100 100.0 Goeldichironomus devineyae 10 2.0 10 20.0 Tanypus neopunctipennis 57 1.0 30 30.0 Tanytarsus sp. 3 2.3 3 6.9 Mollusca Bivalvia Rangia cuneata 2 1.0 1 1.0 Gastropoda Littoridinops sp. 39 2.0 30 60.0 Total 178 225.0 Estuarine Biotic Index = 1.26 Total taxa = 11 Total taxa with sensitivity values = 9 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. Table 1-77. Numbers of benthic macroi nve rte b rates collected in each ponar grab sample at the Huddles Cut downstream station in May 2019. Species/taxon Huddles Cut Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 15 3 15 3 24 12.0 Polychaeta Heteromastus filiformis 6 0 0 0 0 1.2 Marenzelleria viridis 3 3 3 0 3 2.4 Neanthes succinea 6 0 3 0 0 1.8 Arthropoda I nsecta Diptera Chironomus decorus 3 0 0 3 3 1.8 Tanytarsus sp. 0 0 3 0 0 0.6 Malacostraca Amphipoda Gammarus tigrinus 0 0 0 3 0 0.6 Mollusca Gastropoda Littoridinops sp. 0 6 0 0 0 1.2 Total taxa by sample 33 12 24 9 30 Total abundance = 108 Total taxa present = 8 Shannon -Wiener diversity = 1.51 1-87 Table 1-78. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Huddles Cut downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 60 1 30 30.0 Polychaeta Heteromastus filiformis 6 1.1 3 3.3 Marenzelleria viridis 12 2.6 10 26.0 Neanthes succinea 9 1.7 3 5.1 Arthropoda Insecta Diptera Chironomus decorus 9 1.0 3 3.0 Tanytarsus sp. 3 2.3 3 6.9 Malacostraca Amphipoda Gammarus tigrinus 3 2.0 3 6.0 Mollusca Gastropoda Littoridinops sp. 6 2.0 3 6.0 Total I 1 58 1 86.3 Estuarine Biotic Index = 1.49 Total taxa = 8 Total taxa with sensitivity values = 8 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. IW:%3 Table 1-79. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Porter Creek upstream station in May 2019. S ecies/taxon Porter Creek Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Dero sp. 14 3 1 4 4 5.2 Naididae sp. w/ hair 9 0 0 0 0 1.8 Naididae sp. w/o hair 17 0 0 0 0 3.4 Polychaeta Amphicteis floridus 1 0 0 0 0 0.2 Streblospio benedicti 1 0 0 0 0 0.2 Arthropoda Insecta Diptera Chironomus decorus 8 10 8 30 22 15.6 Chironomus stigmaterus 1 0 0 6 0 1.4 Glyptotendipes sp. 1 0 0 0 0 0.2 Nanocladius sp. 0 2 0 0 0 0.4 Parachironomus sp. 3 0 0 6 2 2.2 Procladius sp. 4 2 3 0 0 1.8 Tanypus neopuntipennis 15 4 0 2 0 4.2 Tanytarsus sp. 0 6 4 6 6 4.4 Hemiptera Corixidae sp. 0 0 0 2 0 0.4 Odonata Perethemis tenera 0 2 0 0 0 0.4 Trichoptera Oecetis inconspicua 0 0 0 2 0 0.4 Malacostraca Amphipoda Gammarus tigrinus 50 28 26 48 24 35.2 Decapoda Palaemonetes pugio 1 0 0 0 0 0.2 Isopoda Cassidinidea lunifrons 1 0 0 0 0 0.2 Ostracoda Candonidae sp. 1 0 0 4 4 1.8 Cyprideis littoralis 2 0 1 0 0 0.6 Mollusca Gastropoda Littoridinops sp. 8 0 12 20 2 8.4 Total taxa by sample 137 57 55 130 64 Total abundance = 443 Total taxa present = 21 Shannon -Wiener diversity = 2.08 SRI Table 1-80. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Porter Creek upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 17 1.0 10 10.0 Oligochaeta Dero sp. 26 1.0 10 10.0 Polychaeta Streblospio benedicti 1 1.1 1 1.1 Arthropoda Insecta Diptera Chironomus decorus 78 1.0 30 30.0 Glyptotendipes sp. 1 1.3 1 1.3 Nanocladius sp. 2 2.0 1 2.0 Parachironomus sp. 11 1.1 10 11.0 Procladius sp. 9 1.2 3 3.6 Tanypus neopunctipennis 21 1.0 10 10.0 Tanytarsus sp. 22 2.3 10 23.0 Hemiptera Corixidae sp. 2 1.3 1 1.3 Trichoptera Oecetis inconspicua 2 4.6 1 4.6 Malacostraca Amphipoda Gammarus tigrinus 176 2.0 100 200.0 Isopoda Cassidinidea lunifrons 1 2.0 1 2.0 Mollusca Gastropoda Littoridinops sp. 42 2.0 30 60.0 Polychaeta Terebellida Amphicteis floridus 1 1.5 1 1.5 Total 220 371.4 Estuarine Biotic Index = 1.68 Total taxa = 21 Total taxa with sensitivity values = 17 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. .8 Table 1-81. Numbers of benthic macro invertebrates collected in each ponar grab sample at the Porter Creek downstream station in May 2019. Species/taxon Porter Creek Downstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 0 1 0 0 0 0.2 Marenzellaria viridis 1 0 0 0 0 0.2 Streblospio benedicti 0 1 0 0 0 0.2 Arthropoda Insecta Diptera Chironomus decorus 100 92 100 44 70 81.2 Procladius sp. 3 4 1 0 0 1.6 Tanypus neopuntipennis 3 1 2 0 2 1.6 Tanytarsus sp. 0 0 0 1 0 0.2 Malacostraca Amphipoda Apocorophium sp. 0 0 0 1 0 0.2 Gammarus tigrinus 0 25 3 7 1 7.2 Ostracoda Cyprideis littoralis 0 0 2 0 0 0.4 Mollusca Bivalvia Macoma tenta 1 0 0 0 0 0.2 Gastropoda Littoridinops sp. 3 16 3 0 1 4.6 Total taxa by sample 111 140 111 53 74 Total abundance = 489 Total taxa present = 12 Shannon -Wiener diversity = 0.72 IMsSI Table 1-82. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Porter Creek downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Streblospio benedicti 1 1.1 1 1.1 Marenzellaria viddis 1 2.6 1 2.6 Arthropoda Insecta Diptera Chironomus decorus 406 1.0 100 100.0 Procladius sp. 8 1.2 3 3.6 Tanypus neopunctipennis 8 1.0 3 3.0 Tanytarsus sp. 1 2.3 1 2.3 Malacostraca Amphipoda Apocorophium sp. 1 2.2 1 2.2 Gammarus tigrinus 36 2.0 30 60.0 Mollusca Bivalvia Macoma tenta 1 2.0 1 2.0 Gastropoda Littoridinops sp. 23 2.0 10 20.0 Polychaeta Terebellida Amphicteis floridus 1 1.5 1 1.5 Total 152 198.3 Estuarine Biotic Index = 1.31 Total taxa = 12 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed. 1-92 Table 1-83. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the DCUT11 upstream station in May 2019. Species/taxon DCUT11 Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Limnodrilus hoffineisteri 0 0 0 0 4 0.8 Naididae sp. 0 0 0 3 0 0.6 Naididae sp. w/o hair 0 0 3 0 0 0.6 Polychaeta Amphicteis floridus 3 6 9 45 32 19.0 Arthropoda Collembola Sminthuridae sp. 0 4 0 0 0 0.8 Insecta Coleoptera Berosus sp. 0 0 0 0 2 0.4 Diptera Bezzia/Palpomyia complex 6 0 6 6 0 3.6 Chironomus decorus 3 0 0 3 2 1.6 Dicrotendipes nervosus 18 2 9 6 38 14.6 Goeldichironomus devineyae 0 0 0 0 2 0.4 Nanocladius sp. 3 0 3 3 10 3.8 Orthocladiinae sp. 0 2 3 0 0 1.0 Parachironomus sp. 0 0 0 0 8 1.6 Procladius sp. 0 0 0 0 6 1.2 Tanypus neopunctipennis 0 0 0 3 2 1.0 Tanytarsus sp. 9 0 0 12 4 5.0 Hemiptera Abedus/Belostoma sp. 3 2 0 0 0 1.0 Odonata Enallagma sp. 3 0 0 0 2 1.0 Perithemis tenera 0 0 0 3 2 1.0 Malacostraca Amphipoda Gammarus tigrinus 18 22 12 30 52 26.8 Ostracoda Candonidae sp. 3 8 36 33 8 17.6 Cyprideis littoralis 0 0 0 33 4 7.4 Mollusca Gastropoda Littoridinops sp. 0 0 9 39 6 10.8 Physidae sp. (physalphysella) 0 0 0 3 0 0.6 Total taxa by sample 69 46 90 222 184 Total abundance = 611 Total taxa present = 22 Shannon -Wiener diversity = 2.42 1-93 Table 1-84. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the DCUT11 upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Limnoddlus hoffineisted 4 1.0 3 3.0 Naididae sp. 3 1.0 3 3.0 Naididae sp. w/o hair 3 1.0 3 3.0 Polychaeta Amphicteis floridus 95 2.0 30 60.0 Arthropoda Insecta Coleoptera Berosus sp. 2 1.5 1 1.5 Diptera Bezzia/Palpomyia complex 18 2.3 10 23.0 Chironomus decorus 8 1.0 3 3.0 Dicrotendipes nervosus 73 1.0 30 30.0 Goeldichironomus devineyae 2 2.0 1 2.0 Nanocladius sp. 19 2.0 10 20.0 Parachironomus sp. 8 1.1 3 3.3 Procladius sp. 6 1.2 3 3.6 Tanypus neopunctipennis 5 1.0 3 3.0 Tanytarsus sp. 25 2.3 10 23.0 Hemiptera Abedus/Belostoma sp. 5 1.0 3 3.0 Odonata Enallagma sp. 5 1.5 3 4.5 Malacostraca Amphipoda Gammarus tigrinus 134 2.0 100 200.0 Mollusca Gastropoda Littoridinops sp. 54 2.0 30 60.0 Physidae sp. (physalphysella) 3 1.3 3 3.9 Total 252 452.8 Estuarine Biotic Index = 1.80 Total taxa = 22 Total taxa with sensitivity values = 17 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. Table 1-85. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the DCUT11 downstream station in May 2019. Species/taxon DCUT11 Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Dero sp. 1 0 0 0 0 0.2 Polychaeta Amphicteis floridus 36 100 45 40 3 44.8 Arthropoda Insecta Coleoptera Berosus sp. 0 0 0 4 1 1.0 Diptera Bezzia/Palpomyia complex 5 9 0 0 0 2.8 Chironomus decorus 0 6 0 0 0 1.2 Dicrotendipes nervosus 1 0 0 12 0 2.6 Goeldichironomus devineyae 1 6 0 0 0 1.4 Nanocladius sp. 0 3 0 0 0 0.6 Procladius sp. 1 3 0 0 0 0.8 Tanytarsus sp. 2 6 9 0 0 3.4 Malacostraca Amphipoda Gammarus tigrinus 18 100 69 16 0 40.6 Ostracoda Candonidae sp. 0 0 3 0 3 1.2 Cyprideis littoralis 0 12 6 0 0 3.6 Mollusca Bivalvia Rangia cuneata 0 0 1 0 0 0.2 Gastropoda Littoridinops sp. 3 3 9 4 1 4.0 Total taxa by sample 1 68 1 248 142 1 76 8 Total abundance = 542 Total taxa present = 15 Shannon -Wiener diversity = 1.55 1-95 Table 1-86. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the DCUT11 downstream station in May 2019. Species/taxona Number of individuals" Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Dero sp. 1 1.0 1 1.0 Polychaeta Amphicteis floridus 224 2.0 100 200.0 Arthropoda Insecta Coleoptera Berosus sp. 5 1.5 3 4.5 Diptera Bezzia/Palpomyia complex 14 2.3 10 23.0 Chironomus decorus 6 1.0 3 3.0 Dicrotendipes nervosus 13 1.0 10 10.0 Goeldichironomus devineyae 7 2.0 3 6.0 Nanocladius sp. 3 2.0 3 6.0 Procladius sp. 4 1.2 3 3.6 Tanytarsus sp. 17 2.3 10 23.0 Malacostraca Amphipoda Gammarus tigrinus 203 2.0 100 200.0 Mollusca Bivalvia Rangia cuneata 1 1.0 1 1.0 Gastropoda Littoridinops sp. 20 2.0 10 20.0 Total 257 501.1 Estuarine Biotic Index = 1.95 Total taxa = 15 Total taxa with sensitivity values = 13 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. Table 1-87. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the DCUT19 upstream station in May 2019. Species/taxon DCUT19 Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Limnodrilus hoffineistreri 4 0 0 0 0 0.8 Polychaeta Amphicteis floridus 40 76 12 9 18 31.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 4 4 3 0 6 3.4 Chironomus stigmaterus 8 0 12 0 9 5.8 Dicrotendipes nervosus 4 4 6 0 3 3.4 Goeldichironomus devineyae 4 0 0 0 0 0.8 Goeldichironomus holoprasinus 0 4 0 0 0 0.8 Nanocladius sp. 0 0 3 0 0 0.6 Procladius sp. 4 0 0 0 0 0.8 Tanypus neopuntipennis 4 8 3 6 3 4.8 Tanytarsus sp. 100 100 39 9 42 58.0 Malacostraca Amphipoda Gammarus tigrinus 0 4 0 0 0 0.8 Ostracoda Candonidae sp. 72 24 45 9 27 35.4 Cyprideis littoralis 0 0 0 0 3 0.6 Mollusca Gastropoda Littoridinops sp. 20 20 6 3 12 12.2 Total taxa by sample 1 264 1 244 129 1 36 123 Total abundance = 796 Total taxa present = 15 Shannon -Wiener diversity = 1.78 1-97 Table 1-88. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the DCUT19 upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Oligochaeta Limnoddlus hoffineisted 4 1.0 3 3.0 Polychaeta Amphicteis floridus 7 2.0 3 6.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 17 2.3 10 23.0 Goeldichironomus devineyae 4 2.0 3 6.0 Dicrotendipes nervosus 17 1.0 10 10.0 Nanocladius sp. 3 2.0 3 6.0 Procladius sp. 4 1.2 3 3.6 Tanypus neopunctipennis 24 1.0 10 10.0 Tanytarsus sp. 290 2.3 100 230.0 Malacostraca Amphipoda Gammarus tigrinus 4 2.0 3 6.0 Mollusca Gastropoda Littoridinops sp. 61 2.0 30 60.0 Total 178 363.6 Estuarine Biotic Index = 2.03 Total taxa = 15 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. Table 1-89. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the DCUT19 downstream station in May 2019. S ecies/taxon DCUT19 Downstream Sampies 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 0 4 0 6 12 4.4 Polychaeta Amphicteis floridus 0 0 0 3 4 1.4 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 8 0 4 0 16 5.6 Chironomus decorus 24 8 4 3 40 15.8 Dicrotendipes nervosus 12 0 0 6 24 8.4 Goeldichironomus devineyae 0 0 0 0 4 0.8 Malacostraca Amphipoda Apocorophium lacustre 0 8 0 9 8 5.0 Apocorophium sp. 0 8 0 0 0 1.6 Gammarus tigrinus 100 100 76 100 100 95.2 Isopoda Cyathura polita 4 0 4 0 4 2.4 Ostracoda Candonidae sp. 40 4 8 6 100 31.6 Cyprideis littoralis 0 4 0 0 0 0.8 Mollusca Bivalvia Veneroida Mytilopsis leucophaeata 0 4 0 0 0 0.8 Gastropoda Littoridinops sp. 16 0 4 12 52 16.8 Total taxa by sample 204 140 100 145 364 Total abundance = 953 Total taxa present = 13 Shannon -Wiener diversity = 1.69 Table 1-90. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the DCUT19 downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 22 1.0 10 10.0 Polychaeta Amphicteis floridus 7 2.0 3 6.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 28 2.3 10 23.0 Chironomus decorus 79 1.0 30 30.0 Dicrotendipes nervosus 42 1.0 30 30.0 Goeldichironomus devineyae 4 2.0 3 6.0 Malacostraca Amphipoda Apocorophium lacustre 25 2.2 10 22.0 Apocorophium sp. 8 2.2 3 6.6 Gammarus tigrinus 476 2.0 100 200.0 Isopoda Cyathura polity 12 2.0 10 20.0 Mollusca Bivalvia Mytilopsis leucophaeata 4 1.5 3 4.5 Gastropoda Littoridinops sp. 84 2.0 30 60.0 Total 1 242 418.1 Estuarine Biotic Index = 1.73 Total taxa = 13 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. IMi18I11 Table 1-91. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Duck Creek upstream station in May 2019. Species/taxon Duck Creek Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Dero sp. 0 3 0 0 0 0.6 Naididae sp. w/o hair 2 0 3 12 9 5.2 Arthropoda Insecta Coleoptera Peltodytes sp. 0 0 3 0 0 0.6 Diptera Chironomus decorus 8 3 0 0 0 2.2 Cryptotendipes sp. 2 0 0 0 0 0.4 Dicrotendipes nervosus 0 3 0 0 0 0.6 Goeldichironomus devinyae 0 3 0 0 0 0.6 Kiefferulus sp. 0 0 0 0 3 0.6 Procladius sp. 2 0 0 0 0 0.4 Tanytarsus sp. 0 0 0 0 15 3.0 Hemiptera Corixidae sp. 2 0 0 0 0 0.4 Trichocorixa sexcinta 4 0 0 0 0 0.8 Odonata Perethemis tenera 0 0 0 0 3 0.6 Malacostraca Amphipoda Apocorophium sp. 2 0 0 0 0 0.4 Gammarus tigrinus 28 12 9 3 6 11.6 Ostracoda Candonidae sp. 2 33 9 12 15 14.2 Total taxa by sample 1 52 1 57 24 1 27 51 Total abundance = 211 Total taxa present = 15 Shannon -Wiener diversity = 1.94 1-101 Table 1-92. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Duck Creek upstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae (tubificinae) sp. w/o hair 26 1.0 10 10.0 Oligochaeta Dero sp. 3 1.0 3 3.0 Arthropoda Insecta Diptera Chironomus decorus 11 1.0 10 10.0 Cryptotendipes sp. 2 2.7 1 2.7 Dicrotendipes nervosus 3 1.0 3 3.0 Goeldichironomus devineyae 3 2.0 3 6.0 Procladius sp. 2 1.2 1 1.2 Tanytarsus sp. 15 2.3 10 23.0 Coleoptera Peltodytes sp. 3 1.4 1 1.4 Hemiptera Corixidae sp. 2 1.3 1 1.3 Trichocorixa sexcinta 4 1.0 3 3.0 Malacostraca Amphipoda Apocorophium sp. 2 2.2 1 2.2 Gammarus tigrinus 58 2.0 30 60.0 Total 67 116.8 Estuarine Biotic Index = 1.64 Total taxa = 15 Total taxa with sensitivity values = 12 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-102 Table 1-93. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Duck Creek downstream station in May 2019. Species/taxon Duck Creek Downstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 0 5 5 6 19 7.0 Arthropoda Insecta Diptera Chironomus decorus 22 53 17 34 46 34.4 Clinotanypus sp. 0 0 1 0 0 0.2 Goeldichironomus holoprasinus 1 1 1 0 0 0.6 Kiefferulus sp. 2 1 0 3 2 1.6 Procladius sp. 12 74 25 82 100 58.6 Tanypus neopunctipennis 10 16 0 3 15 8.8 Tanytarsus sp. 0 3 0 3 3 1.8 Malacostraca Amphipoda Gammarus tigrinus 1 1 6 5 1 2.8 Ostracoda Cyprideis littoralis 5 13 13 97 91 43.8 Mollusca Gastropoda Littoridinops sp. 6 6 0 8 11 6.2 Total taxa by sample 59 173 68 241 288 Total abundance = 829 Total taxa present = 11 Shannon -Wiener diversity = 1.65 1-103 Table 1-94. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Duck Creek downstream station in May 2019. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 35 2 30 60.0 Arthropoda Insecta Diptera Chironomus decorus 172 1 100 100.0 Clinotanypus sp. 1 1.4 1 1.4 Procladius sp. 293 1.2 100 120.0 Tanypus neopunctipennis 44 1.0 30 30.0 Tanytarsus sp. 9 2.3 3 6.9 Malacostraca Amphipoda Gammarus tigrinus 14 2.0 10 20.0 Mollusca Gastropoda Littoridinops sp. 31 2.0 30 60.0 Total 1 304 398.3 Estuarine Biotic Index = 1.31 Total taxa = 11 Total taxa with sensitivitv values = 8 a Only those taxa for which a sensitivity value is available are displayed b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-104