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Biological Fidelity Analysis of
Stream Classes
- UPDATE
Funded by: Environmental Defense Fund
Conducted by: RTI International
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Outline of Presentation
Background
Current Status:
–Site selection
–Aquatic biota
–Stream classification
–Preliminary statistical analysis results
Individual species
Community assemblage
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Project Objectives:
To adopt a stream classification system that
represents the distribution of aquatic biota in North
Carolina
–evaluate the 7 stream classes of the Environmental Flow
Specialist (EFS) hydrological stream classification system:
A. Coastal Streams
B. Small Stable Streams
F. Medium Stable Streams
C. Large Stable Streams
E. Large Piedmont Rivers
D. Small Flashy Streams
G. Small Seasonal
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Project Objectives:
To adopt a stream classification system that
represents the distribution of aquatic biota in North
Carolina
–Compare fidelities of aquatic biota to different stream
classification systems
–If necessary, modify the EFS stream classes to more
accurately describe the distribution of biota
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Study Methods:
Aquatic Biota and Datasets:
–Fish
–Benthos
–Aquatic species in Natural Heritage Program database
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Aquatic Biota and Datasets
Aquatic Biota Database
Benthos NCDENR DWQ Benthic Macroinvertebrate
Fish NCDENR DWQ Fish Community, USGS
NAQWA, WRC Trout, WRC Diversity (Gameland
Surveys)
Natural Heritage
Inventory
NCDENR Natural Heritage Program
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Study Methods:
Aquatic Biota and Datasets:
–Fish
–Benthos
–Aquatic species in Natural Heritage Program database
Sites:
–185 NHD+ catchments with USGS gages (used to develop
EFS stream classes)
–~600 NHD+ catchments with stream classes based on
WaterFALL hydrologic data
Aquatic biota data within each NHD+ catchment will
be paired with the assigned stream class
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Study Methods:
Stream Classifications Systems:
–EFS
–McManamay et al. (2011)
–Konrad (in review)
Statistical Analyses:
–Random Forest (individual species)
–Similarity Analyses (communities)
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Current Status:
Site Selection
Aquatic Biota
Stream Classifications
Preliminary Statistical Analyses
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1. Site Selection
185 NHD+ catchments with EFS stream classes
527 NHD+ catchments:
–equal representation of 3 physiographic regions
–“minimally impaired” condition:
Water quality: Excellent, Good or Good-Fair Benthos site
condition
Water quantity (instream flow alteration): total drainage area
of monitoring station is at least twice the size of the total
drainage area of the closest upstream flow alteration
–Within each physiographic region, proportional
representation of streams with and without upstream flow
alterations
–Random selection of qualifying sites
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1. Site Selection
Final number of sites (criteria applied to 185 NHD+
catchments + new sites):
–578:
217 mountains
231 piedmont
130 coastal plain
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1. Site Selection
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2. Aquatic Biota
All monitoring sites within 578 NHD+ catchments:
–Fish, benthos, NHP aquatic species
–30 years of data (1981-2010) assuming stream classes
based on ~ 2010 condition
Aquatic Biota Number of Monitoring Sites
Benthos 636
Fish 238
Natural Heritage
Inventory
656
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3. Stream Classification
Stream classes based on WaterFALL hydrologic
data:
–Generate for all 578 NHD+ catchments:
2006 NLCD
40 years of climate date (1967-2006)
Instream flow alterations (dams, withdrawals and discharges
– 1995-2012)
–Plan to generate these stream classes within the next
couple of months
–Calibrate and QAQC stream classes with a comparison
against classes determined by USGS gage data
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3. Stream Classification
Stream classification systems:
–EFS (7 classes), McManamay et al. (2011) (8 classes)
–Konrad (in review) (8 classes)
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4. Preliminary Statistical Analyses
Analyses:
–Random Forest (individual species)
Presence/absence
–Similarity Analysis (communities)
Presence/absence
Abundance
Dataset:
–Benthos (106 out of 185 NHD+ catchments with EFS
stream classes)
–Taxonomic ambiguity resolved
–Remove Parent – Keep Child (resolved to Genus level)
dataset used in both sets of analyses
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Number of NHD catchments for each stream class
4. Preliminary Statistical Analyses
Stream Class Number of NHD+
Catchments
A14
B41
C6
D30
E1
F8
G 6
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Random Forest is a decision-tree modeling and classification
approach (Cutler et al., 2007)
A decision tree is a predictive model that uses a set of binary
rules (yes/no) to split the data based on the predictor variable
Random Forest Approach
All Stream Class
and Aquatic
Biology Data
Stream Class A
Species 1
present
Species 2
present
Species 3
present
Species 4
present
YesYes No No
Stream Class B
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Random Forest Analysis -Biological fidelity
to stream class
= high probability
= medium probability
= low probability
= absent
Aquatic
species
Stream Class
ABCDEFG
1
2
3
4
5
6
7
8
9
10
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= high probability
= medium probability
= low probability
= absent
Random Forest Analysis –NO Biological
fidelity to stream class
Aquatic
species
Stream Class
ABCDEFG
1
2
3
4
5
6
7
8
9
10
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4. Preliminary Statistical Analyses
Low
Medium High
Importance:ABCDEFG
Ceratopsyche 0.032 0.034 0.005 0.076 0.000 0.012 0.032
Serratella 0.031 0.008 ‐0.003 0.071 0.000 0.000 ‐0.010
Heptagenia 0.013 ‐0.003 0.034 0.025 0.000 0.001 0.010
Pycnopsyche ‐0.012 0.011 ‐0.006 0.019 0.000 0.004 0.027
Phylocentropus 0.038 0.001 0.001 0.002 0.000 0.000 0.005
Cardiocladius 0.000 ‐0.004 ‐0.004 0.001 0.000 0.016 0.001
Pteronarcys 0.007 0.000 0.013 0.019 0.000 0.004 0.004
Neophylax 0.014 0.008 0.011 0.013 0.000 ‐0.006 ‐0.015
Paragnetina 0.004 0.005 0.008 0.013 0.000 0.000 0.007
Brachycentrus ‐0.006 0.000 ‐0.001 0.013 0.000 ‐0.005 0.004
Promoresia 0.007 0.000 ‐0.003 0.012 0.000 0.004 0.008
Epeorus 0.008 0.005 0.008 0.011 0.000 ‐0.005 0.009
Microtendipes ‐0.010 0.006 0.010 0.011 0.000 ‐0.008 0.010
Taxa
Stream Class
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4. Preliminary Statistical Analyses
Only ~10% of taxa (41 out of 408) showed a higher
fidelity to one stream class over another
Probability of general occurring in each stream class are
probably low
Some of the results may be an artifact of the low
numbers of NHD+ catchments for each stream class (1-
41)
Minimum number of NHD+ catchments for more robust
analyses is ~ 40.
36% of taxa had < 3 records
Fidelities could be further confounded by including all
benthos sites in the analysis
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Questions?