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HomeMy WebLinkAbout20080868 Ver 2_Section III G Benthos 2021 PCS Creeks Report_20220605G. BENTHOS This section contains more detailed information in support of the response to Question 3 in Section II. Section II-C Question 3 contains benthos information on total abundance and species richness comparisons and conclusions drawn from multivariate evaluations of all creek study data. Section II-C Question 3 also includes discussion of benthic guilds and trophic levels. Appendix A, Section H contains a history of benthic macroinvertebrate collections and a description of methods (field, laboratory, and statistics). In -situ hydrographic and water quality data collected prior to each collection of 2021 are included at the end of Appendix I (on flash drive only); ECU water quality data are presented in Appendix E (on flash drive only). Note that ECU water quality collection sites are various distances upstream from benthos collections sites. Water quality data are discussed in Section II-C Question 6 and Section III-C. Appendix I also contains raw benthic data collected for each sweep and ponar grab in 2021 for all sampled creeks. 1.0 Results and Discussion of Sweep and Ponar Data Figures III-G1 through III-G12 show dendrograms of species community composition for each creek sample location and type (e.g., upstream or downstream and sweep or ponar) that exhibited statistically significant groupings. Thus, in this comparison, each stream had four different analyses: upstream -sweeps, downstream -sweeps, upstream-ponars, and downstream-ponars. When appropriate, additional cluster analysis of all taxa and comparisons of results from each particular creek to control creeks were included. It should be noted that clusters with different colored lines on the cluster dendrograms represent non -significant group structure among factors (e.g., years, creeks) at the five percent level (P = 0.05). Tables III-G1 through III-G5 display summary information for all monitored creeks for comparison. Table III-G1 contains the cumulative list of the 405 benthic taxa collected in ponars or sweeps across the 23 years of the study and the NCDWR-assigned sensitivity values for 190 of them (46.9 percent of total taxa). Tables III-G2 and III-G4 show the four most abundant upstream and downstream taxa per year in each creek. Table III-G3 and Table III-G5 show the benthic community structure within all creeks, including averages for each creek and the range of averages for upstream and downstream sites for each creek across all years. The structure of benthic assemblages in estuarine environments responds to many stressors that freshwater and marine assemblages seldom encounter. Common estuarine stressors include frequent and often extreme fluctuations in environmental conditions, particularly salinity, dissolved oxygen, and temperature. Typically, these stressors result in a more tolerant taxa compared to more stable freshwater or marine waters. However, the upstream communities in many of the study creeks (e.g., SCUT1, Little Creek, Jacobs Creek, Huddles Cut, DCUT11, and Duck Creek) are subject to frequent periods of lower water levels and lower flow regimes during portions of their life cycle. The sensitivity values range from 1 to 5, with 1 being most tolerant/insensitive and 5 being most intolerant/sensitive (relative to environmental stressors). For the sensitivity value cumulative list shown in Table III-G1, over the 23 years the average value of all taxa (with assigned sensitivity values) shown is 1.9. The most intolerant taxa at 5.0 (e.g., Mysidae, Metamysidopsis swifti) represented at 0.25 percent of the total taxa and species with a value of 1.0 (e.g., Naididae, Dero digitata) represented 8 percent of the total taxa recorded. The overall sensitivity value of 1.9 for all taxa was influenced largely by the assemblage of taxa found in six orders which comprised 22 percent of all taxa encountered. Five of these orders (Diptera, Hemiptera, Coleoptera, Amphipoda, Odonata) were in the phylum Arthropoda and one order (Haplotaxida) was in the phylum Annelida. III-G-1 Summary data for sweep data by each upstream and downstream site during the course of the study are presented by year in Table III-G3. Annual average and range of averages are presented in these tables for four parameters: 1) total abundance, 2) total taxa, 3) total taxa with sensitivity values, and 4) EBI (estuarine biotic index). Highest values for these parameters were recorded in 1998, 2008, or 2011. Summary data for all ponar data by each upstream and downstream site during the course of the study are presented by year in Table III-G5. Annual average and range of averages are presented in these tables for five parameters: 1) total abundance, 2) total taxa. 3) total taxa with sensitivity values, 4) Shannon -Wiener index, and 5) EBI (estuarine biotic index). Highest values for these parameters were scattered within two periods: 1998-2004 and 2012- 2018. To further evaluate an aspect of the community structure, the highest values and range of highest values for the Shannon -Wiener Index and EBI are presented for each upstream and downstream creek site in Table III-G6. As has been the consistent case, the downstream samples had the highest percentage of years with the highest Shannon -Wiener Diversity score (69.6 percent), highest EBI ponar score (69.6 percent), and highest EBI sweep score (78.3 percent). When the highest average scores for the three measures are compared, out of the 15 creeks, the downstream samples had 10 of 15 for Shannon -Wiener, 10 of 15 for EBI ponar grabs, and 11 of 15 for EBI sweeps. A majority of the highest scores across all three measures occur in the downstream samples (50 compared to 20 upstream), which has been the trend since 2016. The four creeks with the most years of data and their respective percentage of high scores were: Huddles Cut with 23 years (42.6 percent), Jacks Creek with 14 years (24.6 percent), Muddy Creek with 12 years (17.4 percent), and Tooley Creek with five years (10.4 percent). The other creeks ranged from 0 to 3 years. Among all the creeks, Huddles Cut had the highest percent of monitored years with the highest EBI scores for both ponar grabs (seven years; 38.9 percent) and sweeps (11 years; 61.1 percent) and the highest percent of years with the highest Shannon -Weiner Diversity score (five years; 27.8 percent). For the ponar grabs, Huddles Cut had the highest EBI score in seven of the 18 years, five of which were after- all drainage basin reduction. The following report sections present results on each creek divided into three parts: a) creeks with pre -Mod Alt L data, b) creeks with pre- and post -Mod Alt L data, and c) control creeks. Within each part, for each creek and sample location, the sweep data discussion precedes the ponar data discussion. a. Pre -Mod Alt L Creeks Sweep and Ponar Grab Data Broomfield Swamp Creek (2019-2021) According to the similarity profile test (SIMPROF), there were no clusters among the three years of upstream or downstream sweeps and ponars collected in Broomfield Swamp Creek. The 2021 macroinvertebrate collection for Broomfield Swamp Creek was the third pre -Mod Alt L sample. For the sweeps, the number of dominants identified to species was four for both upstream and downstream, two of which were the same (Table III-G2). Total abundance, total taxa, and EBI were highest in the downstream sample (Table III-G3). For the ponars, the number of dominants identified to species to date was six upstream and seven downstream (Table III-G4) and the total abundance, total taxa, Shannon -Wiener Index, and EBI were highest in the downstream sample (Table III-G5). III-G-2 Upstream sweeps were dominated by four different species (Mytilopsis leucophaeta and species of Corixidae, Tanytarsus, and Naididae without hair) in 2021 (Table III- G2). Community structure comparisons for the upstream sweeps show that species richness was 26 (16 in 2020), total abundance was 264 individuals (809 in 2020), and the EBI score was 1.31 (1.54 in 2020) in 2021 (Table III-G3). Downstream sweeps dominants were Gammarus tigrinus Dicrotendipes nervosus, and species of Naididae without hair and Tanytarsus in 2021 (Table III- G2). The community structure comparisons for the downstream sweeps show that species richness was 29 (17 in 2020), total abundance was 380 individuals (821 in 2020), and the EBI score was 1.48 in 2021 (1.64 in 2020) (Table III-G3). Upstream ponar grabs contained Chironomus decorus, Tanypus neopunctipennis and species of Naididae without hair and Procladius (Table III-G4). In 2021 the total abundance was 297 individuals (87 in 2020), species richness was 12 (5 in 2020), the Shannon -Weiner Index was 1.76 (1.21 in 2020), and the EBI score was 1.03 (1.22 in 2020) (Table III-G5). Ponar grab dominants from downstream were Dicrotendipes nervosus, Amphicteis floridus, Limnodrilus hoffmeisteri, and species of Naididae without hair in 2021 (Table III-G4). In 2021, total abundance was 37 (144 in 2020), species richness was 11 (10 in 2020), the Shannon - Weiner Index was 2.02 (1.16 in 2020), and the EBI was 1.20 (1.91 in 2020) (Table III-G5). b. Post -Mod Alt L Creeks Sweep and Ponar Grab Data Jacks Creek (1998-2005; 2011-2021) The 2021 macroinvertebrate collection for Jacks Creek was the 19th sample year and seventh post -Mod Alt L year. Drainage basin reduction in Jacks Creek ceased in 2015. For the sweeps to date, the number of dominants identified to species for upstream was 12 and 13 for downstream; in which three were only upstream and four downstream (Table III- G2). Sweep community structure metrics for both upstream and downstream years are in Table III-G3. For the ponars to date, the number of dominants identified to species upstream was 12 and 15 for downstream; in which five were only upstream and eight downstream (Table III-G4). Ponar community structure metrics for both upstream and downstream by year are in Table III- G5. i. Jacks Creek Upstream Sweeps Multivariate cluster analysis by SIMPROF of benthic taxa richness and abundance from upstream Jacks Creek sweeps revealed significant variation between all years among six distinct clusters; with 2021 grouped in the largest cluster (Figure III-G1a). Clusters A (1999) and B (1998) each consisted of a single year. Cluster C consisted of two pre years (2011 and 2013), D consisted of four pre years (2000, 2001, 2004, and 2005), E consisted of 2014-2021, and F consisted of three pre years (2012, 2002, and 2003). Seven post -Mod Alt L years are clustered together in E with a one earlier pre -Mod Alt L year (2014). The other clusters consisted of all pre -Mod Alt L years. Comparison of interannual variability between clusters by similarity percentages (SIMPER) revealed similarity ranged from 31.2 to 55.5 percent. Clusters B and F were the least similar (31.2 percent similarity) with differences predominantly from the absence of Ablabesmyia peleensis, and species of Cricotopus and Cladotanytarsus in cluster F. Sweeps from upstream Jacks Creek were consistently dominated by species of Apocorophium (seven out of 19 years) and Littoridinops (18 out of 19 years), along with Gammarus tigrinus (11 out of 19 years), and Chironomus decorus (eight out of 19 years); no new dominant species have appeared since 2012 (Table III-G2). Community structure comparisons for the upstream sweeps indicate that species richness ranged from 18 taxa (1999) to 42 taxa (1998) and total abundance ranged from 783 (1999) to 3,529 individuals (2011) (Table III-G-3 III-G3). The 2021 samples contained 901 individuals among 31 taxa. The EBI scores ranged from 1.59 (2014) to 2.07 (2013); the 2021 score was 1.92 (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance from upstream Jacks Creek sweeps by similarity percentages (SIMPER) revealed 51.0 percent similarity with differences driven predominantly by higher abundances of Corixidae species and species of Apocorophium and Tanytarsus in the post -Mod Alt L years (2015-2021). Comparison of interannual variability among upstream sweeps by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L upstream communities. ii. Jacks Creek Downstream Sweeps The SIMPROF test of benthic taxa richness and abundance of the downstream sweeps from Jacks Creek revealed significant variation between all years among five distinct clusters (Figure III-G1 b). Comparison of interannual variability between groups by means of similarity percentages (SIMPER) revealed similarity ranged from 17.6 to 47.9 percent. Clusters A (1998), B (2021), and C (2002) each consisted of one year. Cluster D consisted of years 2011-2014 and 2020, and E consisted of the remaining years (1999-2001, 2003-2005 and 2015-2019). Earlier pre -Mod Alt L years are mixed among clusters A-D. Sweeps collected from downstream Jacks Creek consistently contained Gammarus tigrinus (14 out of 19 years) and species of Littoridinops (18 out of 19 years), Apocorophium (13 out of 19 years), and Hargeria rapax (5 out of 19 years); 2018 was the last year a new dominant appeared (Table III-G2). Community structure comparisons for the downstream sweeps collected indicate that species richness ranged from 19 (1999 and 2021) to 45 taxa (1998) and total abundance ranged from 304 (2021) to 2,477 individuals (1998) (Table III-G3). The 2021 samples had the lowest total abundance to date (304 individuals) among the lowest number of taxa (19) since 1999. The 2021 EBI score was 1.98 (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within the downstream Jacks Creek sweeps by similarity percentages (SIMPER) revealed 47.6 percent similarity with differences driven predominantly by higher abundances of Dicrotendipes nervosus in the post -Mod Alt L years. Comparison of interannual variability between the downstream Jack Creek sweeps by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. iii. Jacks Creek Upstream Ponar Grabs The SIMPROF test of benthic taxa richness and abundance within the upstream Jacks Creek ponar grabs revealed slight variation of statistical significance between all years among three distinct clusters (Figure III-G1c). Cluster A consisted of one year (2002), but the other clusters consisted of a mixture of pre and post years. Comparison of interannual variability between the clusters by means of similarity percentages (SIMPER) indicated that clusters A and C had the lowest similarity percentage (17.5 percent similarity); which were driven predominantly by the absence in species of Macoma, greater abundance in species of Chironomus, and lower abundance in species of Littordinops in C. The 19 years of data revealed that upstream ponar grabs of Jacks Creek consistently contained Gammarus tigrinus (15 out of 19 years), Chironomus decorus (12 out of 19 years), and Amphicteis floridus (11 out of 19 years) as dominant species. In 2021, Laeonereis culveri is a dominant for the first time in Jacks upstream (Table III-G4). Community III-G-4 structure comparisons for the upstream ponar grabs indicate that species richness ranged from five (2012) to 30 taxa (2004) and total abundance ranged from 90 (2016) to 1,356 individuals (2001). In 2021, total abundance was 188 individuals among 15 taxa (Table III-G5). The Shannon - Wiener diversity scores ranged from 0.72 (1999) to 2.33 (2011) with the 2021 score at 1.67. The EBI scores ranged from 1.07 (2012) to 2.00 (1999), with the 2021 score at 1.18. Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance from upstream Jacks Creek ponar grabs by similarity percentages (SIMPER) revealed 38.4 percent similarity with differences driven predominantly by higher abundances of Gammarus tigrinus and lower abundances of Chironomus species and Amphicteis floridus in the post -Mod Alt L years. Comparison of interannual variability between the upstream Jacks Creek ponar grabs by ANOSIM detected no spatial differences of statistical significance between pre - and post -Mod Alt L macroinvertebrate communities. iv. Jacks Creek Downstream Ponars The SIMPROF test of benthic taxa richness and abundance within the downstream Jacks Creek ponar grabs revealed slight variation of statistical significance between years among three distinct clusters; which each cluster has a mixture of pre and post years (Figure III-G1d). Comparison of interannual variability between the clusters by means of similarity percentages (SIMPER) indicated that clusters A and B had the lowest percent similarity (25.2); differences were predominantly due to higher abundances of Gammarus tigrinus, Mediomastus ambiseta, and Eteone heteropoda in B. Ponar grabs from downstream Jacks Creek often contained Chironomus decorus as a dominant; although it was not one of the four most abundant genera in 2016-2018 and 2020; no new dominant species appeared in eight of the 19 years, the last was in 2018. Other dominant species included Gammarus tigrinus (10 out of 19 years), Streblospio benedicti (nine out of 19 years), and species of Chironomus (six out of 19 years) (Table III-G4). Community structure comparisons for the downstream ponar grabs indicate that species richness ranged from 11 (2000) to 25 taxa (2015) and total abundance ranged from 183 (2014) to 1,639 individuals (2003). The 2021 samples contained 564 individuals among 10 taxa. The Shannon - Wiener diversity scores ranged from 0.96 (2019) to 2.51 (2014) and the EBI scores ranged from 1.11 (2012) to 2.33 (2016); the 2021 Shannon -Wiener diversity score was the lowest of 0.80 and the EBI score was 1.20 (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance from downstream Jacks Creek ponar grabs by similarity percentages (SIMPER) revealed 34.1 percent similarity with differences driven predominantly by lower abundances in species of Chironomus, Tubificoides, and Mediomastus, and higher abundances in Gammarus tigrinus, and species of Apocorophium in the post -Mod Alt L years. Comparison of interannual variability between the downstream Jacks Creek ponar grabs by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. Jacobs Creek (2011-2021) The 2021 benthic macroinvertebrate collections for Jacobs Creek represented the 11th consecutive year and the eighth post -Mod Alt L. Drainage basin reductions in Jacobs Creek ceased in 2015. For the sweeps to date, the number of dominants identified to species upstream and downstream was eight and nine, respectively; of those species three were found only upstream and four were found only downstream (Table III-G2). Sweep community structure metrics for upstream and downstream by year are in Table III-G3. For the ponars to III-G-5 date, the number of dominants identified to species upstream was nine with 12 for downstream; of those species four were found only upstream and seven only downstream (Table III-G4). Ponar community structure metrics for upstream and downstream by year are in Table III-G5. i. Jacobs Creek Upstream Sweeps According to SIMPROF, there were no clusters among the 11 years of upstream sweeps collected in Jacobs Creek. Sweep dominants from upstream Jacobs Creek typically were species of Littoridinops (11 out of 11 years), Apocorophium (eight out of 11 years), and Chironomus (five out of 11 years). Palemonetes pugio is considered a new dominant for 2021 (Table III-G2). Community structure comparisons for the upstream sweeps indicate that species richness ranged from 19 (2012) to 34 taxa (2016) and total abundance ranged from 718 (2020) to 1,678 individuals (2011). The 2021 samples contained the second lowest total abundance (792 individuals) among 27 taxa. The EBI scores range from 1.63 (2017) to 2.40 (2012) with an EBI score of 1.92 in 2021 (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance in upstream Jacobs Creek sweeps by similarity percentages (SIMPER) revealed 49.1 percent similarity which was predominately driven by more species of Tanytarsus and Chironomus in post -Mod Alt L years. Comparison of interannual variability between Jacobs Creek upstream sweeps by ANOSIM detected statistical significance between pre- and post -Mod Alt L macroinvertebrate communities (R = 0.449, P = 0.02). ii. Jacobs Creek Downstream Sweeps The SIMPROF test of benthic taxa richness and abundance within the downstream Jacobs Creek sweeps revealed slight variation of statistical significance between all years among three distinct clusters (Figure III-G2). Cluster A contained post -Mod Alt L years (2015-2017, 2019, 2021), while the other two clusters contained a mixture of pre and post years. Comparison of interannual variability between the clusters by means of similarity percentages (SIMPER) indicated clusters A and C had the lowest percent similarity of 48.2. Differences were driven predominantly by a higher abundance of Littoridinops tenuipes in C and higher abundances of Gammarus tigrinus in A. Sweeps from downstream Jacobs Creek consistently contained species of Apocorophium (nine out of 11 years), Littoridinops (10 out of 11 years), and Gammarus (seven out of 11 years) and Hargeria rapax (six out of 11 years). There were no new species in 2021 (Table III-G2). Community structure comparisons for the downstream sweeps indicate that species richness ranged from 29 (2013 and 2016) to 35 taxa (2014 and 2015), total abundance ranged from 890 (2021) to 2,198 individuals (2018) and EBI scores ranged from 1.80 (2017) to 2.10 (2011) (Table III-G3). The 2021 samples contained 32 taxa and had an EBI score of 1.85. Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance of Jacobs Creek downstream sweeps by similarity percentages (SIMPER) revealed 54.4 percent similarity with differences driven predominantly by higher abundance of Gammarus tigrinus and lower abundances for species of Apedilum for the post -Mod Alt L years (2014 — 2021). Comparison of interannual variability between Jacobs Creek downstream sweeps by ANOSIM detected no statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. III-G-6 N. Jacobs Creek Upstream Ponar Grabs According SIMPROF, there were no clusters among the 10 years of upstream ponar grabs from Jacobs Creek. Ponar grabs from upstream Jacobs Creek contained Chironomus decorus as a dominant (seven out of 11 years) with Apocorophium species as an occasional dominant (six out of 11 years); Laeonereis culveri was a new dominant species in 2021 (Table III- G4). Community structure comparisons for the ponar grabs from upstream indicate that species richness was highest in 2015 (23) after the 2014 and 2019 lows of 15 taxa, and total abundance ranged from 59 (2016) to 1,277 individuals (2013) (Table III-G5). The 2021 upstream samples contained the third lowest total abundance (170 individuals) among the lowest species richness (12 taxa). Shannon -Wiener diversity scores ranged from 1.45 (2021) to 2.48 (2020) and EBI scores range from 1.13 (2021) to 2.27 (2013). The 2021 upstream ponar grabs had the lowest Shannon -Wiener diversity score and EBI. Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within the upstream Jacobs Creek ponar grabs by similarity percentages (SIMPER) revealed 38.1 percent similarity. Comparison of interannual variability between Jacobs Creek upstream ponar grabs by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. iv. Jacobs Creek Downstream Ponar Grabs According to SIMPROF, there were no clusters among the 10 years of ponar grabs collected from downstream Jacobs Creek. Ponar grabs dominants were typically Chironomus decorus (eight out of 11 years) and species of Apocorophium (four out of 11 years) and Gammarus (five out of 11 years); three of the 10 years had no new dominant species (2012, 2018, and 2020) (Table III- G4). Species richness ranged from 12 (2012) to 24 taxa (2011) and total abundance ranged from 213 (2016) to 877 individuals (2017). The 2021 samples had 480 individuals among 15 taxa, the Shannon -Wiener diversity score was the lowest (0.47), and the EBI score was the lowest (1.11) in the 11 years of Jacobs Creek monitoring (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance of Jacobs Creek downstream ponar grabs by similarity percentages (SIMPER) revealed 39.1 percent similarity with differences driven predominantly by the absence of Apedilum species and lower abundances of Chironomus species, Parachironomus species, and Apocorophium species for the post -Mod Alt L years (2014-2021). Rangia cuneata was present only in post -Mod Alt L years although the species was also collected pre -mod Alt L by ponar within another nearby creek (2000 in Tooley Creek). Comparison of interannual variability between Jacobs Creek downstream ponar grabs by ANOSIM detected no statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. Drinkwater Creek (2011-2021) The 2021 benthos samples for Drinkwater Creek represented the 11th consecutive year of benthic macroinvertebrate collections and the ninth post -Mod Alt L year; drainage basin reduction in the Drinkwater Creek watershed ceased in 2014. For the sweeps to date, the number of dominants identified to species was seven for upstream and eight for downstream; among those species, three were found only upstream and four only downstream (Table III-G2). Sweep community structure metrics for upstream and downstream by year are in III-G-7 Table III-G3. For the ponars to date, the number of dominants identified to species was eight upstream and 12 downstream; among those species, two were found only upstream and six only downstream (Table III-G4). Ponar community structure metrics for upstream and downstream Drinkwater Creek by year are in Table III-G5. i. Drinkwater Creek Upstream Sweeps Multivariate cluster analysis by a similarity profile test (SIMPROF) of benthic taxa richness and abundance within the upstream Drinkwater Creek sweeps revealed slight variation of statistical significance between all years among three distinct clusters (Figure III-G3a). Comparison of interannual variability between the clusters by means of similarity percentages (SIMPER) indicated that cluster A contained one pre year and seven post years (2011, 2013, 2015-2019), B contained one year (2020), and C contained one pre and post year (2012 and 2014). Clusters A and B had the lowest SIMPER (51.6), with differences predominantly due to higher abundance of Littoridinops tenuipes and Trichocorixa sexcinta in cluster B. Sweeps from upstream Drinkwater Creek consistently were dominated by species of Littoridinops (10 out of 11 years) and Apocorophium (10 out of 11 years); no new dominant species have appeared since 2019. (Table III-G2). Community structure comparisons for the upstream sweeps collected indicate that species richness ranged from 22 (2015 and 2020) to 34 (2011), total abundance ranged from 596 individuals (2019) to 2,032 individuals (2011), and EBI scores ranged from 1.75 (2014) to 2.25 (2011) (Table III-G3). The 2021 EBI score was tied with 2020 EBI (1.82), while the total abundance was 1,250 individuals among 28 taxa (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L upstream benthic sweep taxa composition and abundance from Drinkwater Creek by similarity percentages (SIMPER) revealed 56.3 percent similarity with differences driven predominantly by higher abundance of Gammarus tigrinus and lower abundances in species of Dasyhelea and Apedilum for the post -Mod Alt L years (2013-2021). Comparison of interannual variability between Drinkwater Creek upstream sweeps by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. ii. Drinkwater Creek Downstream Sweeps Multivariate cluster analysis by a similarity profile test (SIMPROF) test of benthic taxa richness and abundance within the downstream Drinkwater Creek sweeps revealed slight variation of statistical significance between all years among two distinct clusters (Figure III-G3 b). Comparison of interannual variability between the clusters by means of similarity percentages (SIMPER) indicated that cluster A contained 2020 only, while cluster B contained the remaining years (2011-2019 and 2021). Differences between the two clusters were due to higher abundance of Littoridinops tenuipes, Trichocorixa sexcinta, and species of Corixidae in A. Sweeps from downstream Drinkwater Creek consistently were dominanted by species of Littoridinops (11 out of 11 years) and Apocorophium (eight out of 11 years) along with Gammarus tigrinus (seven out of 11 years); no new dominant species appeared since 2019 (Table III-G2). Species richness ranged from 24 (2020) to 40 (2016), total abundance ranged from 884 individuals (2019) to 1,785 individuals (2015), EBI scores ranged from 1.73 (2020) to 2.04 (2011 and 2019). In 2021 the total abundance was 970 individuals among 25 taxa and the EBI score was 1.76 (Table III-G3). Comparison of interannual variability between pre- and post -Mod III-G-8 Alt L downstream benthic sweep taxa composition and abundance from Drinkwater Creek by similarity percentages (SIMPER) revealed 56.2 percent similarity with differences driven predominantly by lower abundances of Americamysis almyra and Palaeomonetes pugio and higher abundances of Gammarus tigrinus for the post -Mod Alt L years (2013-2021). Comparison of interannual variability between Drinkwater Creek downstream benthic sweeps by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. Drinkwater Creek Upstream Ponar Grabs According to SIMPROF, there were no clusters among the 11 years of ponar grabs collected from upstream Drinkwater Creek. Ponar grabs within upstream Drinkwater Creek consistently were dominanted by species of Chironomus (eight out of 11 years) and Amphicteis floridus (nine out of 11 years) (Table III-G4). Species richness ranged from 11 (2019) to 23 taxa (2013), total abundance ranged from 242 (2020) to 1,267 individuals (2012) (Table III-G5). The 2021 total abundance was 343 individuals among 20 taxa, Shannon -Wiener diversity score was 1.78 (range: 1.58 — 2.32), and the EBI score was 1.74 (range: 1.59 — 2.10) (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L upstream benthic ponar taxa composition and abundance within Drinkwater Creek by similarity percentages (SIMPER) revealed 52.9 percent similarity with differences driven predominantly by the absence of Aulodrilus sp. and the lower abundances of Macoma balthica and Littordinops species in the post -Mod Alt L years (2013 — 2021). Comparison of interannual variability by ANOSIM detected no spatial differences of statistical significance between Drinkwater Creek upstream ponar pre- and post -Mod Alt L macroinvertebrate communities. iv. Drinkwater Creek Downstream Ponar Grabs Multivariate cluster analysis by SIMPROF of benthic taxa richness and abundance from downstream Drinkwater Creek ponar grabs revealed significant variation between all years among five different clusters (Figure III-G3 c). Cluster A consisted of one pre - Mod Alt L year (2012), B contained two post -Mod Alt L years (2019 and 2021), C contained one year (2015), D contained two post -Mod Alt L years (2016 and 2017), and E contained the remaining years (2011, 2013, 2014, 2018, and 2020). Comparison of interannual variability between clusters by similarity percentages (SIMPER) ranged between 13.2 and 52.8 percent, with clusters A and D being less similar predominately due to greater abundance of species of Chironomus and Apedilum. Ponar grabs within downstream Drinkwater Creek usually were dominated by species of Chironomus (eight out of 11 years), Mediomastus ambiseta (seven out of 11 years) and Streblospio benedicti (seven out of 11 years) as dominants; there have been no new dominant species since 2019 (Table III-G4). Species richness ranged from 12 (2021) to 23 taxa (2018 and 2020) and total abundance ranged from 161 (2015) to 1,140 individuals (2013). The 2021 samples had 508 individuals, the lowest Shannon -Wiener diversity score of 0.59 (previous range: 1.34 — 2.46), and the lowest EBI score of 1.09 (previous range: 1.57 — 2.23) (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L downstream benthic ponar taxa composition and abundance within Drinkwater Creek by similarity percentages (SIMPER) revealed 34.2 percent similarity with differences driven predominantly by the absence of Apedilum sp. and lower abundances of Parachironomus sp., III-G-9 and Macoma balthica in the post -Mod Alt L years (2013 — 2021). Comparison of Drinkwater Creek downstream interannual variability by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L downstream ponar macroinvertebrate communities. Tooley Creek (1998 — 2001; 2010 - 2021) The 2021 benthos samples for Tooley Creek represented the 16th year of benthic macroinvertebrate collections and was the 10th year considered post -Mod Alt L (2012 -2021); drainage basin reduction in the Tooley Creek watershed ceased in 2013. For the sweeps to date the number of dominants identified to species was 11 for upstream and nine for downstream; among those species four were only upstream and three were only downstream (Table III-G2). Sweep community structure metrics for upstream and downstream are in Table III-G3. For the ponars to date, 15 dominants were identified to species in the upstream and 14 downstream; among those species four were found only upstream and four were found only downstream (Table III-G4). Ponar community structure metrics for upstream and downstream are in Table III-G5. i. Tooley Creek Upstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance within upstream Tooley Creek by SIMPROF revealed slight variation of statistical significance between years among five distinct clusters (Figure III-G4a). Cluster A and E contained all pre -Mod Alt L years (A: 1998 and 1999; E: 2000 and 2001), B contained a mixture of pre- and post -Mod Alt L years, C contained only one year (2019), and D contained all post -Mod Alt L years (2015-2017 and 2021). Comparison of interannual variability between the clusters by similarity percentages (SIMPER) indicated that cluster A and B were the least similar (36.1 percent); differences were due to higher abundances in Gammarus tigrinus and Amphicteis floridus in cluster A. Sweeps within upstream Tooley Creek were dominated by Littoridinops species every year and species of Apocorophium every year since 2010 except for 2015; no new dominants have appeared since 2015 (Table III-G2). Community structure comparisons for the upstream sweeps indicate that species richness ranged from 17 (1999) to 42 taxa (1998), total abundance ranged from 918 (2010) to 2,572 individuals (2017), and EBI scores range from 1.80 (2015 and 2018) to 2.08 (2001 and 2012) (Table III-G3). In 2021, total abundance was 1,962 individuals among 24 taxa with an EBI score of 1.95 (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L upstream sweep taxa composition and abundance within Tooley Creek by similarity percentages (SIMPER) revealed 49.9 percent similarity with differences driven predominantly by lower abundances of Gammarus tigrinus and Cassidinidea lunifrons, and higher abundances of both Nematoda species and Hargeria rapax in the post -Mod Alt L years (2012 — 2021). Comparison of interannual variability between upstream sweeps by ANOSIM detected spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities (R = 0.272, P = 0.02). ii. Tooley Creek Downstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance within downstream Tooley Creek by a similarity profile test (SIMPROF) revealed slight variation of statistical significance between years among five distinct clusters (Figure III-G4 b). Cluster A and B both consisted of one year (1998 and 2020, respectively), cluster C of three pre - Mod Alt L years (1999-2001), cluster D contained all post -Mod Alt L years (2015-2019 and 2021), and E a mixture of pre- and post -Mod Alt L years (2010-2014). As determined by a SIMPER III-G-10 analysis, clusters A and B were the least similar at 22.2 percent and differed based on the absence of Chironomus species and Enallagma species in B. Sweeps within downstream Tooley Creek consistently were dominated by species of Littoridinops (15 out of 16 years) and Apocorophium (13 out of 16 years), and Hargeria rapax (10 out of 16 years); no new dominants have appeared since 2014 (Table III- G2). Gammarus tigrinus, abundant in the first four years (1998 — 2001), was again abundant in 2015-2021. Species richness ranged from 18 (2020) to 47 taxa (1998), total abundance ranged from 321 (2020) to 3,360 individuals (1998), and EBI scores ranged from 1.80 (2019) to 2.07 (2014) (Table III-G3). In 2021, total abundance was 899 individuals among 22 taxa and the EBI score was 1.92 (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L downstream sweeps taxa composition and abundance within Tooley Creek by SIMPER revealed 50.8 percent similarity with differences driven predominantly by higher abundances of Gammarus mucronatus and lower abundances of Cyprideis littoralis in the post -Mod Alt L years (2012-2021). Comparison of interannual variability in Tooley Creek downstream sweeps by ANOSIM detected spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities (R = 0.234, P = 0.04). iii. Tooley Creek Upstream Ponar Grabs Multivariate cluster analysis of benthic ponar taxa richness and abundance within upstream Tooley Creek by SIMPROF revealed slight variation of statistical significance between years among six distinct clusters (Figure III-G4 c). Cluster A consisted of two post -Mod Alt L years (2016 and 2020), B of three pre -Mod Alt L years (1998, 1999, and 2001), C of one year (2010), D of three years (2000, 2012, and 2021), E of one year (2013), and F of one pre -Mod Alt L year and five post -Mod Alt L years (2011, 2014-2015, and 2017-2019). Comparison of interannual variability between clusters by similarity percentages (SIMPER) revealed similarity ranged from 17.5 to 42.6 percent. Ponar grabs within upstream Tooley Creek often were dominated by Chironomus decorus (11 out of 16 years), species of Gammarus (eight out of 16 years), and species of Apocorophium (six out of 16 years). Americamysis almyra was a dominant for the first time in 2021 (Table III-G4). Community structure comparisons indicate that species richness ranged from 11 (2000 and 2012) to 24 (2001) and total abundance ranged from 50 (2020) to 942 (2013). In 2021, the total abundance was 316 individuals among 14 taxa, the Shannon -Wiener diversity score was 1.22 (range: 0.49 — 2.43), and the EBI score was 1.25 (range: 1.15 — 2.09) (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L benthic ponar taxa composition and abundance within upstream Tooley Creek by similarity percentages (SIMPER) revealed 34.5 percent similarity with differences driven predominantly by lower abundances of Gammarus tigrinus, Amphicteis floridus, and Macoma balthica in the post - Mod Alt L years (2012 — 2021). Comparison of interannual variability in Tooley Creek upstream ponar grabs by ANOSIM detected no spatial differences of statistical significance between pre - and post -Mod Alt L macroinvertebrate communities. iv. Tooley Creek Downstream Ponar Grabs Multivariate cluster analysis of benthic ponar taxa richness and abundance within downstream Tooley Creek by a similarity profile test (SIMPROF) revealed III-G-11 variation of statistical significance between years among two clusters (Figure III-G4 d). Cluster A contained three pre -Mod Alt L years (1998-2000), while cluster B contained the remainder. Ponar grabs within downstream Tooley Creek frequently were dominanted by Mediomastus ambiseta (every year since 2011) and Chironomus decorus, although Chironomus decorus was not among the four most dominant species in 2015-2018. Streblospio benedicti was a dominant for the past three years; 2021 did not contain any new dominant species (Table III-G4). Species richness ranged between 11 (2000 and 2021) and 25 (2001) and total abundance ranged between 270 individuals (1999) and 1,415 individuals (2012 (Table III-G5). The 2021 samples contained 511 individuals, the Shannon -Wiener diversity score was the lowest 0.68 (previous range: 1.00 — 2.31) and the EBI score was 1.16 (range: 1.16 — 2.07) (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L ponar benthic taxa composition and abundance within downstream Tooley Creek by similarity percentages (SIMPER) revealed 36.8 percent similarity with differences driven predominantly by higher abundances of Streblospio benedicti, Mediomastus ambiseta, Eteone heteropoda, and species of Chironomus in the post -Mod Alt L years (2012 — 2021). Comparison of interannual variability of Tooley Creek downstream ponar grabs by ANOSIM detected spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities (R = 0.335, P = 0.006). Huddles Cut (1999-2001; 2007-2021) The 2021 benthos samples for Huddles Cut represented the 18th year of benthic macroinvertebrate collections and the 12th year considered post -Mod Alt L (2010 — 2021); drainage basin reduction from mine activities in the Huddles Cut watershed ceased in 2011. For the sweeps to date the number of dominants identified to species was nine for upstream and 16 for downstream; among those species, four were only upstream and 11 were only downstream (Table III-G2). Community structure metrics for upstream and downstream sweeps are in Table III-G3. For the ponars to date the number of dominants identified to species was 16 for upstream and 18 for downstream; of those species five were only upstream and six were only downstream (Table III-G4). Community structure metrics for upstream and downstream ponars are in (Table III-G5). i. Huddles Cut Upstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance within upstream Huddles Cut by SIMPROF revealed statistically significant variation between years among five distinct clusters (Figure III-G5 a). Cluster A contained one year (1999), B contained two pre -Mod Alt L years (2000 and 2001), C contained two post -Mod Alt L years (2012 and 2020), while D and E contained a mixture of pre- and post -Mod Alt L years. Comparison of interannual variability between clusters by similarity percentages (SIMPER) revealed clusters A and C were least similar (28.3 percent) predominately driven by greater abundance in Gammarus tigrinus, and species of Littordinops in A. Sweeps within upstream Huddles Cut often were dominated by Cyprideis littoralis (14 out of 18 years) and Palaeomonetes pugio every year except 2015 and 2021 (Table III-G2). Community structure comparisons for the upstream sweeps within Huddles Cut indicate that species richness ranged from 13 (2009) to 29 taxa (2018), total abundance ranged from 219 (2021) to 1,647 individuals (2016), and EBI scores ranged from 1.72 (2021) to 2.22 (2009); 2021 had 17 taxa and the lowest total abundance (219 individuals) and lowest EBI score (1.72) to date (Table III-G3). III-G-12 Comparison of interannual variability between pre- and post -Mod Alt L Huddles Cut upstream sweep taxa composition and abundance by SIMPER revealed 47.7 percent similarity. Differences were due predominantly to higher abundances of Gammarus tigrinus and species of Chironomus and Apocorophium, and lower abundances of species of Littoridinops and Tanytarsus in the post -Mod Alt L years (2010 — 2021). Comparison of interannual variability among upstream sweeps by ANOSIM revealed spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities within Huddles Cut (R = 0.365; P = 0.006). ii. Huddles Cut Downstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance within downstream Huddles Cut by a similarity profile test (SIMPROF) revealed slight variation of statistical significance between years between two distinct clusters (Figure III-G5 b). Cluster A contained four sample years (1999, 2000, 2001, and 2017) and B consisted of the other 13 years (2007-2016, 2018-2021). Comparison of interannual variability between the clusters by similarity percentages (SIMPER) indicated 39.2 percent similarity. Sweeps in downstream Huddles Cut consistently contained Gammarus tigrinus (a dominant every year but 2001) and Palaeomonetes pugio (a dominant 14 out of 18 years), with species of Apocorophium as an occasional dominant (10 out of 18 years); Dicrotendipes nervosus was a new dominant in 2021 (Table III-G2). Species richness ranged from 12 (2011) to 33 taxa (2017), total abundance ranged from 75 (2011) to 1,057 individuals (2017), EBI scores ranged from 1.74 (2008) to 2.36 (2020); 2021 sweeps had 141 individuals among 15 taxa and an EBI score of 1.93 (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within downstream Huddles Cut sweeps by SIMPER revealed 43.4 percent similarity with differences driven predominantly by higher abundances of Gammarus tigrinus, Gammarus mucronatus, and species of Apocorophium in the post -Mod Alt L years (2010 — 2021). Comparison of interannual variability among the Huddles Cut downstream sweeps by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. iii. Huddles Cut Upstream Ponar Grabs Multivariate cluster analysis of taxa richness and abundance in Huddles Cut upstream ponar grabs by SIMPROF revealed slight variation of statistical significance between years among four distinct clusters (Figure III-G5 c). Comparison of interannual variability between the clusters by SIMPER indicated similarity ranged from 16.9 to 34.7 percent. Cluster A contained four years (2009, 2010, 2019, and 2021), B contained five post -Mod Alt L years (2012-2014, 2018, and 2020), C contained three years (1999, 2001, and 2016), and D contained a mixture of pre- and post -Mod Alt L years (2000, 2007, 2008, 2011, 2015, and 2017). Ponar grabs within upstream Huddles Cut contained various dominant species over the years, but Chironomus decorus and Cyprideis littoralis (both eight out of 18 years), were often one of the top four with other species as dominant for several years (Table III-G4). Species richness ranged from three (2010) to 25 taxa (2018) and total abundance ranged from 46 (2000) to 596 individuals (2016); 2021 ponars contained 178 individuals among nine taxa, the Shannon -Wiener diversity score was 0.43 (range: 0.32 — 2.52), and the EBI score was 1.09, the lowest to date (previous range: 1.11 — 1.98) (Table III-G5). III-G-13 Comparison of interannual variability between pre- and post -Mod Alt L upstream ponar grabs taxa composition and abundance within Huddles Cut by SIMPER revealed 31.2 percent similarity with differences driven predominantly by higher abundances of Heteromastus filiformis, species of Chironomus, and Macoma tenta, and lower abundances of Cyprideis littoralis and Amphicteis floridus in the post -Mod Alt L years (2010- 2021). Comparison of interannual variability among Huddles Cut upstream ponar grabs by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. iv. Huddles Cut Downstream Ponar Grabs Multivariate cluster analysis of ponar grabs taxa richness and abundance within downstream Huddles Cut by a similarity profile test (SIMPROF) revealed slight variation of statistical significance between years among four distinct clusters (Figure III-G5 d). Comparison of interannual variability between the clusters by similarity percentages (SIMPER) indicated similarity ranged from 6.2 to 24.7 percent. Cluster A and B each contained only one year (2021 and 2015, respectively), cluster B contained seven years (1999-2001, 2007, 2010, 2016, and 2017), and C contained nine years (2008, 2009, 2011-2014 and 2018-2020). Ponar grabs within downstream Huddles Cut contained various dominants over the years, but Chironomus decorus (eight out of 18 years) was usually one of them with Lepidactylus dytiscus (four out of 18 years), Heteromastus filiformis (three out of 18 years), and Neanthes succinea (three out of 18 years) as occasional dominants; no new dominant species since 2017 (Table III-G4). Species richness ranged from seven (2015) to 24 taxa (2001 and 2017) and total abundance ranged from 108 (2019) to 1,980 individuals (2013). The 2021 ponars contained 121 individuals among eight taxa, the Shannon -Wiener diversity score was 1.26 (range: 1.24 — 2.56), and the EBI score was 1.55 (range: 1.13 — 3.08) (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L downstream benthic taxa composition and abundance within Huddles Cut ponar grabs by SIMPER revealed 25.4 percent similarity with differences driven predominantly by higher abundances of Nematoda species and Mediomastus ambiseta and lower abundances of Chironomus species and Amphicteis floridus in the post -Mod Alt L years (2010-2021). Comparison of interannual variability among the Huddles Cut downstream ponar grabs by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. Porter Creek (2011-2021) The 2021 benthic macroinvertebrate collection in Porter Creek was the 11th consecutive year of Mod Alt L samples and the sixth year considered post -Mod Alt L. For the sweeps to date the number of dominants identified to species was 10 upstream and 10 for downstream; among those species, five were only upstream and another five were only downstream (Table III-G2). Community structure metrics for upstream and downstream sweeps are in Table III-G3. For the ponars to date, the number of dominants identified to species was nine for upstream and 10 for downstream; among those species, five were only upstream and six were only downstream (Table III-G4). Community structure metrics for upstream and downstream ponars are in Table III-G5. i. Porter Creek Upstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance within upstream Porter Creek by SIMPROF revealed statistically significant variation III-G-14 between years among three distinct clusters (Figure III-G6 a). Cluster A contained one year (2019), B contained three years (2015-2017), C contained the remaining years. Comparison of interannual variability by SIMPER revealed that clusters A and B were the least similar (45.1 percent) with differences driven predominantly by the absence of Dero species and less abundance in species of Tanytarsus in B. Sweeps from upstream Porter Creek contained Gammarus tigrinus, a dominant every year, while other frequent dominants were species of Littoridinops (nine out of 11 years) and Cyprideis littoralis (six out of 11 years). Goeldichironomus devineyae was a dominant for the first time in 2021 (Table III-G2). Species richness for the upstream sweeps ranged from 21 (2015 and 2020) to 34 taxa (2012), total abundance ranged from 765 (2016) to 2,804 individuals (2012), and EBI scores ranged from 1.85 (2011) to 2.05 (2013). The 2021 sweeps had 832 individuals among 33 taxa, and an EBI score of 1.86 (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within upstream Porter Creek sweeps by SIMPER revealed 53.5 percent similarity with differences driven predominantly by lower abundances of species of Apocorophium and Tanytarsus, Dicrotendipes nervosus and Cyathura polita in the four post -Mod Alt L years (2016- 2020). Comparison of interannual variability among the Porter Creek upstream sweeps by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate. ii. Porter Creek Downstream Sweeps The SIMPROF test of benthic sweeps taxa richness and abundance from downstream Porter Creek revealed slight variation of statistical significance between years among four distinct clusters (Figure III-G6 b). Cluster A and B both contained one year (2021 and 2012, respectively), cluster C contained three years (2016, 2017, and 2019), and D contained the other six years (2011, 2013 — 2015, 2018, and 2020). Clusters A and D had the lowest similarity (30.5 percent) predominately due to the absence of Littoridinops tenuipes, Gammarus mucronatus, and species of Chironomus in A. Sweeps from downstream Porter Creek always were dominated by species of Apocorophium (9 out of 11 years) and consistently contained species of Littoridinops (10 out of 11 years) and Gammarus (10 out of 11 years), and these genera were the three most abundant in 2016-2020 (Table III-G2). Species richness ranged from 16 (2021) to 33 taxa (2013), total abundance ranged from 180 (2021) to 1,791 individuals (2011), and EBI scores ranged from 1.82 (2020) to 2.18 (2012). In 2021, total abundance and total taxa was the lowest (180 individuals among 16 taxa) and an EBI of 2.06 (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within downstream Porter Creek sweeps by SIMPER revealed 49.7 percent similarity with differences driven predominantly by lower abundances of Hargeria rapax, Gammarus mucronatus, Palaeomonetes pugio, Polydora cornuta, and species of Chironomus in the post -Mod Alt L years (2016-2021). Five years post -Mod Alt L, comparison of interannual variability among the Porter Creek downstream sweeps by ANOSIM detected spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities (R = 0.301; P = 0.01). III-G-15 N. Porter Creek Upstream Ponar Grabs According to SIMPROF, there were no clusters among the 11 years of upstream ponar grabs from Porter Creek. Ponar grabs from upstream Porter Creek contained Gammarus tigrinus as a dominant species for all sampled years except 2012 with species of Apocorophium and Amphicteis floridus as frequent co -dominants (Table III-G4). Community structure comparisons for the upstream ponar grabs indicate that species richness ranged from 11 (2021) to 22 taxa (2011) and total abundance ranged from 232 (2021) to 2,136 individuals (2012). In 2021, ponars had the lowest total abundance (232 individuals) and the lowest species richness (11 taxa). The Shannon -Wiener diversity score was 1.68 (range: 1.53 — 2.30), while the EBI was 1.73 (previous range: 1.68 — 2.06 (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within upstream Porter Creek ponar grabs by SIMPER revealed 48.3 percent similarity with differences driven predominantly by lower abundances of species of Tanytarsus and Apocorophium in the five post -Mod Alt L years (2016- 2021). Comparison of interannual variability among the upstream ponars by ANOSIM detected no spatial difference of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. iv. Porter Creek Downstream Ponar Grabs According to SIMPROF, there were two clusters among the eight years of ponar grabs collected from downstream Porter Creek. Cluster A contained two post -Mod Alt L years (2019 and 2021), while cluster B contained the remaining years. Comparison of interannual variability by SIMPER revealed that 32.6 percent similarity between the two clusters with differences driven predominantly by higher abundances of Chironomus sp. And lower abundances of Streblospio benedicti in B. Ponar grabs from downstream Porter Creek usually were dominated by species of Chironomus (eight out of 11 years) and Macoma (seven out of 11 years), with Mediomastus ambiseta (five out of 11 years), Streblospio benedicti (six out of 11 years), and Gammarus tigrinus (four out of 11 years) being less frequent dominants (Table III-G4). Species richness ranged from nine (2021) to 19 taxa (2013) and total abundance ranged from 123 (2012) to 743 individuals (2017). In 2021, ponar grabs had 356 individuals among the lowest species richness (9 taxa), the lowest Shannon -Wiener diversity score of 0.49 (previous range: 0.79 — 2.03) and the lowest EBI score of 1.14 (previous range: 1.20 — 1.94) (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within downstream Porter Creek ponar grabs by SIMPER revealed 38.8 percent similarity with greater abundance of Chironomus species and Macoma species predominately driving differences in post years (2016-2021). Comparison of interannual variability among the Porter Creek downstream ponars by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. DCUT11 (2013-2021) The 2021 benthic macroinvertebrate collection in DCUT11 was the ninth consecutive year of Mod Alt L samples with 2021 being the fourth post -Mod Alt L year. For the sweeps to date the number of dominants identified to species was six for upstream and five for downstream; among those species two were found only upstream and one was found only III-G-16 downstream (Table III-G2). Community structure metrics for upstream and downstream sweeps are in Table III-G3. For the ponar grabs to date the number of dominants identified to species was five upstream and nine downstream; among those species one was found only upstream and four were found only downstream; no species were only upstream while five were only downstream (Table III-G4). Community structure metrics for upstream and downstream ponars are in Table III-G5. i. DCUT11 Upstream Sweeps According to SIMPROF, there were no clusters among the nine years of upstream sweeps from DCUT11. Sweeps from upstream DCUT11 consistently contained Gammarus tigrinus (a dominant species each year except 2021), Cyprideis littoralis (every year except 2013, 2019, and 2021), and species of Littoridinops (every year except 2019). There has been no new dominant species since 2017 (Table III-G2). Species richness has ranged from 22 (2016) to 30 taxa (2019) while abundance ranged from 421 (2021) to 1,750 individuals (2015). The 2021 taxa count was 29 and the EBI score was 1.52 (range: 1.46-2.07) (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within upstream DCUT11 sweeps by similarity percentages (SIMPER) revealed 59.3 percent similarity. Differences between pre- and post- Mod Alt L included higher abundances of Littoridinops tenuipes and species of Apocorophium in post - Mod Alt L years (2018-2021). Comparison of interannual variability among the DCUT11 upstream sweeps by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. ii. DCUT11 Downstream Sweeps Multivariate cluster analysis of benthic sweeps taxa richness and abundance within downstream DCUT11 by a similarity profile test (SIMPROF) revealed statistically significant variation between years between two distinct clusters (Figure III-G7 a). Cluster A contained a mixture of pre- and post -Mod Alt L years (2013-2018, and 2020) and B contained two post -Mod Alt L years (2019 and 2021). Comparison of interannual variability between clusters by similarity percentages (SIMPER) revealed that these two clusters were 48.1 percent similar with differences driven predominantly by higher abundances of Amphicteis floridus, Cyprideis littoralis, and species of Apocorophium in A. Like the upstream, downstream sweeps were consistently dominated by Gammarus tigrinus (every year except 2021), along with species of Littoridinops (every year), and usually Cyprideis littoralis (2014-2018). Other dominant species included Tanytarsus species (a dominant species since 2016). There were no new dominant species since 2015 (Table III-G2). Community structure comparisons show that species richness was lowest in 2018 (19 taxa) and highest in 2019 (32 taxa). Total abundance was lowest in 2021 (374 individuals) and highest in 2015 (2,205). In 2021, the taxa count was 24 and EBI score was the lowest 1.50 (previous range 1.65 — 2.03) (Table III-G3). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within downstream DCUT11 sweeps by similarity percentages (SIMPER) revealed 58.1 percent similarity. Differences between pre- and post- Mod Alt L years include the absence of Nematoda species and higher abundances of Littoridinops tenuipes in post Mod -Alt L years (2018-2021). Comparison of interannual variability among the DCUT11 downstream sweeps by ANOSIM detected no spatial differences of statistical III-G-17 significance between pre- and post -Mod Alt L macroinvertebrate communities. DCUT11 Upstream Ponar Grabs Multivariate cluster analysis of benthic sweeps taxa richness and abundance within upstream DCUT11 by a similarity profile test (SIMPROF) revealed statistically significant variation between years between two distinct clusters (Figure III-G7 b). Cluster A contained one year (2021), while B contained the remaining years (2013-2020). Comparison of interannual variability between clusters by similarity percentages (SIMPER) revealed that these two clusters were 23.2 percent similar. Ponar grabs from upstream DCUT11 consistently contained Gammarus tigrinus (a dominant every year except 2015), species of Littoridinops (a dominant each year except 2019), and Cyprideis littoralis (a domiannt every year except 2013, 2019, and 2021). The last new dominant species appeared in 2019 (Table III-G4). The total abundance was lowest in 2021 (104 individuals) and highest in 2014 (2,192 individuals), while the lowest species richness was in 2021 (10 taxa) and highest in 2019 (22 taxa). In 2021, the Shannon -Weiner Index was 2.04 (range: 1.17-2.42) and EBI score was 1.82 (range: 1.80-2.01) (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within upstream DCUT11 ponar grabs by SIMPER revealed 46.6 percent similarity. Comparison of interannual variability among the DCUT11 upstream ponar grabs by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. iv. DCUT11 Downstream Ponar Grabs According to SIMPROF, there were no clusters among the eight years of ponar grabs collected from downstream DCUT11. Ponar grabs from downstream DCUT11 were consistently dominated by Gammarus tigrinus (dominant each year). Other dominants were species of Apocorophium (a dominant five out of nine years) and Amphicteis floridus (a dominant every year except 2018 and 2020). No new dominant species have appeared since 2019 (Table III-G4). Total abundance was lowest in 2016 (248 individuals) and highest in 2020 (2,105 individuals) while species richness was lowest in 2013 (14 species) and highest in 2014 (28 species). In 2021, total abundance was 624 individuals among 22 taxa. Shannon -Weiner Index was 1.91 (range: 1.31-2.19), and the EBI score was 1.73 (range: 1.67-2.07) (Table III-G5). Comparison of interannual variability between pre- and post -Mod Alt L benthic taxa composition and abundance within downstream DCUT11 ponar grabs by SIMPER revealed 48.7 percent similarity. Comparison of interannual variability among the DCUT11 downstream ponar grabs by ANOSIM detected no spatial differences of statistical significance between pre- and post -Mod Alt L macroinvertebrate communities. c. Control Creeks Sweep and Ponar Grab Data SCUT1 (2019-2021) According to SIMPROF, there were no clusters among the three years of upstream or downstream sweeps and ponars collected in SCUT1. The 2021 macroinvertebrate collection for SCUT1 was the third sample year. For the sweep collection, the number of dominants identified to species was five for III-G-18 upstream and six for downstream; among those species three species were found only upstream and four only downstream (Table III-G2). Community structure metrics for upstream and downstream sweeps are in Table III-G3. For the ponars to date, the number of dominants identified to species was five for upstream and six for downstream; among those species two were found only upstream and four only downstream (Table III-G4). Community structure metrics for ponars are in Table III-G5. Upstream sweeps were dominated each year by species of Naididae without hair, but Corixidae was only present in 2019 and 2021 (Table III-G2). Community structure comparisons for the 2021 sweeps collected from upstream SCUT1 showed that species richness was lowest in 2020 (20 taxa) and highest in both 2019 and 2021 (28 taxa), while total abundance was lowest in 2019 (401 individuals) and highest in 2020 (944 individuals), In 2021, there was 579 individuals among 28 taxa. The EBI score was 1.25 (range: 1.18-1.53) (Table III- G3). Downstream sweeps were dominated by Gammarus tigrinus all three years, Amphicteis floridus being a dominant for the second year, and two other species present only one year (Dicrotendipes nervosus and species of Littoridinops) (Table III-G2). Community structure comparisons showed the lowest species richness in 2020 (18 taxa) and highest in 2019 (35 taxa), while total abundance was lowest in 2021 (446 individuals) and highest in 2020 (997 individuals). In 2021, species richness was 28 taxa. The EBI score was 1.83 (range: 1.72-1.98) (Table III-G3). Ponar grabs from upstream SCUT1 contained species of Naididae without hair all three years, while the three other taxa were only present one year (Procladius sp., Tubificoides heterochaetus, and Chironomus decorus) (Table III-G4). The total abundance was the lowest in 2020 (334 individuals) and highest in 2019 (509 individuals) while species richness was lowest in 2020 (7 taxa) and highest in 2019 (18 taxa). The Shannon -Weiner Index was 2.02 (previous range: 0.99-1.72) and the EBI score was 1.05 (previous range: 1.23-1.74) (Table III- G5). Cyprideis littoralis was a dominant for first two years in ponar grabs from downstream SCUT1 while Amphicteis floridus and Gammarus tigrinus were dominants for both 2020 and 2021 ((Table III-G4). The total abundance was lowest in 2020 (92 individuals) and the highest was in 2021 (277), while species richness was lowest in 2020 and 2021 (8 taxa) and highest in 2019 (16 taxa). The Shannon -Weiner Index was 1.00 (previous range: 1.74-2.13), and the EBI was 1.96 (previous range: 1.25-1.77) (Table III-G5). Little Creek (2011-2021) The 2021 benthos samples for Little Creek represented the 11th consecutive year of Mod Alt L benthic macroinvertebrate collections. For the sweeps to date, the number of dominants identified to species was eight for upstream and nine for downstream; among those species three were found only upstream and four only downstream (Table III-G2). Community structure metrics for upstream and downstream sweeps by year are in Table III-G3. For the ponars to date the number of dominants identified to species was six for upstream and 12 for downstream; among those species three were found only upstream and nine were found only downstream (Table III-G4). Community structure metrics for upstream and downstream ponars are in Table III-G5. i. Little Creek Upstream Sweeps Multivariate cluster analysis of sweep taxa richness and abundance within upstream Little Creek by SIMPROF revealed slight variation of statistical significance between years among four distinct clusters (Figure III-G8 a). Comparison of interannual variability between the clusters by SIMPER indicated similarity ranged from 20.4 to 53.7 percent. Cluster A and B consisted of only one year (2012 and 2019, respectively), C consisted of two years (2011 and 2013), and D consisted of seven years (2014-2018, 2020, and 2021). The two least similar III-G-19 clusters were A and B (20.4 similarity percentage), with differences being driven by the absence of Corixidae species, and higher abundances in Enchytraeidae species and Tanytarsus species in A. Dominants in sweeps within upstream Little Creek contained Gammarus tigrinus every year except 2012 and Cyprideis littoralis from 2015-2020, while species of Littoridinops were dominant six out of 11 years. There were no new dominant species in 2021 (Table III-G2). Total abundance ranged from 404 (2021) to 2,002 individuals (2013), while species richness ranged from 15 (2020) to 29 taxa (2017 and 2021). The EBI score was 1.42 (previous range: 1.50 to 1.92) (Table III-G3). ii. Little Creek Downstream Sweeps Multivariate cluster analysis of sweep taxa richness and abundance within downstream Little Creek by SIMPROF revealed slight variation of statistical significance between years among four distinct clusters (Figure III-G8 b). Cluster A and B consisted of one year (2021 and 2018, respectively), C consisted of two years (2011 and 2013), and D consisted of the remainder of years. Comparison of interannual variability between the clusters by SIMPER ranged from 25.5 to 48.8. Sweeps within downstream Little Creek contained species of Littoridinops every year except 2013 and species of Apocorophium every year except 2019 and 2021 as dominants, while Gammarus tigrinus was among the four most abundant species every year since its first appearance as a dominant in 2015; no new dominant species since 2019 (Table III-G2). Species richness ranged from 16 (2021) to 36 taxa (2012 and 2019), total abundance ranged from 59 (2021) to 1,502 individuals (2017), and EBI scores ranged from 1.79 (2017) to 2.25 (2013) (Table III-G3). The 2021 EBI score was 2.04. iii. Little Creek Upstream Ponar Grabs Multivariate cluster analysis of taxa richness and abundance within downstream Little Creek ponar grabs by SIMPROF revealed slight variation of statistical significance between years among two distinct clusters (Figure III-G8 c). Cluster A contained one year (2020) and B contained the other eight (2011-2019, and 2021). The SIMPER test revealed that the two clusters were 26.9 percent similar. Ponar grabs within upstream Little Creek usually contained Gammarus tigrinus (every year except 2012 and 2021), Amphicteis floridus, and Chironomus decorus as dominants, with Limnodrilus hoffmeisteri present as a dominant for the first time in 2021 (Table III-G4). Species richness ranged from 6 (2020) to 23 taxa (2013) and total abundance ranged from 69 (2020) to 1,865 individuals (2013) (Table III-G5). In 2021, the total abundance was 534 individuals among 15 taxa. The Shannon -Wiener diversity score in 2021 was 1.32 (range: 1.08 — 2.27) and the EBI score was 1.49 (range: 1.38 — 2.00). iv. Little Creek Downstream Ponar Grabs Multivariate cluster analysis of taxa richness and abundance within downstream Little Creek ponar grabs by SIMPROF revealed slight variation of statistical significance between years among three distinct clusters (Figure III-G8 d). Cluster A contained two years (2019 and 2021), B contained 2012, and C contained the remainder years (2011, 2013- 2018, and 2020). The SIMPER test revealed that the three clusters ranged from 27.6 to 33.3 percent similarity. III-G-20 Ponar grabs within downstream Little Creek often included Mediomastus ambiseta (five out of 11 years), Streblospio benedicti (seven out of 11 years), and species of Apocorophium (five out of 11 years) as dominants; Tanypus neopunctipennis was a new dominant species in 2021 (Table III-G4). Species richness ranged from 11 (2019 and 2021) to 28 taxa (2013) and total abundance ranged from 354 (2020) to 2,023 individuals (2013). In 2021, total abundance was 539 individuals, Shannon -Wiener diversity score was the lowest at 0.38 (previous range: 0.75 — 2.40), and EBI score was the lowest at 1.06 (previous range: 1.24 — 1.98) (Table III-G5). PA2 (2011-2021) The 2021 benthos samples for PA2 represented the 11th consecutive year of Mod Alt L benthic macroinvertebrate collections. For the sweeps to date, the number of dominants identified to species was six for both upstream and downstream; among those species one was found only upstream and another was found only downstream (Table III-G2). The upstream and downstream sweeps had matched dominants in four years (2014, 2015, 2017 and 2020) (Table III-G2). Community structure metrics for upstream and downstream sweeps by year are in Table III-G3. For the ponars to date the number of dominants identified to species was nine for both upstream and downstream; among those species one was found only upstream and downstream (Table III-G4). Community structure metrics for upstream and downstream ponars by year are in Table III-G5. i. PA2 Upstream Sweeps Multivariate cluster analysis of sweeps taxa richness and abundance within upstream PA2 by SIMPROF revealed slight variation of statistical significance between years between two distinct clusters (Figure III-G9 a). Cluster A contained four years (2011-2014) and B contained seven years (2015-2021). The SIMPER test showed that both clusters were 52.5 percent similar, with differences predominantly driven by higher abundances of Gammarus tigrinus, species of Corixidae, and Trichocorixa sexcinta in B. Sweeps within upstream PA2 always contained Littoridinops and Apocorophium species (except 2013) as dominants, with Goeldichironomus devineyae (every year except 2011, 2016, 2017, and 2020) and Gammarus tigrinus (only five out of 11 years) less frequently; no new dominant species have appeared since 2014 (Table III-G2). Species richness ranged from 19 (2020) to 31 taxa (2019), total abundance ranged from 977 (2014) to 2,040 individuals (2016), EBI scores ranged from 1.70 (2019) to 2.23 (2012). In 2021 total abundance was 1,121 individuals among 27 taxa and an EBI score of 1.74 (Table III-G3). ii. PA2 Downstream Sweeps According to SIMPROF, there were five distinct clusters (Figure III- G9 b). Cluster A consisted of one year (2020), B consisted of two years (2016 and 2017), C consisted of three years (2015, 2019, and 2021), D consisted of one year (2012), and D consisted of four years (2011, 2013-2014 and 2018). The SIMPER test showed that clusters ranged from 50.2 to 69.1 percent similarity; clusters C and D were the least similar with the absence of Littordinops species and Apedilum species predominately driving differences in C. Sweeps within downstream PA2 contained Apocorophium species each year, Littoridinops species each year except 2018, and often also had Goeldichironomus devineyae (every year except 2017, 2018, and 2020) as dominants; no new dominants since 2018 (Table III-G2). Species richness ranged from 21 (2013) to 31 taxa (2019), total abundance ranged from 1,123 (2014) to 2,183 individuals (2018), and EBI scores ranged from 1.75 (2021) to 2.33 (2012). In 2021, total abundance was 1,132 individuals among 30 taxa and EBI score was the III-G-21 lowest (1.75) (Table III-G3). PA2 Upstream Ponar Grabs According to SIMPROF, there were no clusters among the 11 years of ponar grabs collected from upstream PA2. Ponar grabs within upstream PA2 usually contained Littoridinops species (every year except 2013, 2016, and 2018) and Chironomus decorus (every year except 2015, 2017, and 2020) as dominants; Laeonereis culveri was a new dominant in 2021 (Table III- G4). Species richness ranged from seven (2012) to 19 taxa (2017) and total abundance ranged from 305 (2014) to 784 individuals (2011). In 2021, total abundance was 374 individuals among 14 taxa, Shannon -Wiener diversity score was 1.35 (range: 1.07-2.28), and the EBI score was the lowest at 1.18 (previous range: 1.42-2.87) (Table III-G5). iv. PA2 Downstream Ponar Grabs Multivariate cluster analysis of ponar grabs taxa richness and abundance within downstream PA2 by SIMPROF revealed slight variation of statistical significance between years among four distinct clusters (Figure III-G9 c). Cluster A contained (2020), B contained two years (2016 and 2017), and C contained four years (2015, 2018, 2019, and 2021), and D contained the remainder years (2011-2014). The SIMPER test showed that similarity ranged from 25.5 to 44 percent. Ponar grabs within downstream PA2 usually had species of Littoridinops (each year except 2011, 2016, and 2019) as a dominant, while other dominants frequently included Chironomus decorus (seven out of 11 years) and Laeonereis culveri (two out of 11 years), and Amphicteis floridus (four out of 11 years. There were no new dominant species in 2014 and 2018-2021 (Table III-G4). Community structure comparisons for the ponar grabs within downstream PA2 indicate that species richness ranged from 11 (2014) to 27 (2015 and 2017) and total abundance ranged from 137 individuals (2020) to 1,392 individuals (2018). The 2021 ponars contained 21 taxa and total abundance of 762. The Shannon -Wiener diversity score was 2.11 (range: 1.15 — 2.46) and the EBI score was 1.47 (range: 1.38 — 2.82) (Table III-G5). Long Creek (2011-2021) The 2021 benthos samples for Long Creek represented the 11 th consecutive year of benthic macroinvertebrate collections. For the sweeps to date the number of dominants identified to species was eight for both upstream and downstream; among those species five were found only upstream and five were found only downstream (Table III-G2). Community structure metrics for upstream and downstream sweeps are in Table III-G3. For the ponars to date the number of dominants identified to species was 11 for upstream and 12 for downstream; among those species one was found only upstream and two were only downstream (Table III-G4). Community structure metrics for upstream and downstream ponars by year are in Table III-G5. i. Long Creek Upstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance within upstream Long Creek by SIMPROF revealed slight variation of statistical significance between years among five distinct clusters (Figure III-G10 a). Cluster A, B, and C all had one year each (2020, 2012, and 2019, respectively), D consisted of five years (2015-2018, and 2021), and E consisted of three years (2011, 2013, and 2014). Comparison of interannual variability by means of similarity percentages (SIMPER) ranged from 18.2 to 58.0 percent. Clusters A and C were least similar with a percent similarity of 18.2, which was predominately III-G-22 from the absence of species of Chironomus and Tanytarsus in A. Sweeps within upstream Long Creek usually contained species of Apocorophium (every year except 2013 and 2019) and Littoridinops (every year except 2014), and often Chironomus decorus (every year except 2017, 2018, and 2020) or species of Gammarus (five out of 11 years) as dominants; no new dominant species in 2021 (Table III-G2). Species richness ranged from 21 (2020) to 40 taxa (2019), total abundance ranged from 250 (2020) to 2,930 individuals (2019), and EBI scores ranged from 1.64 (2019) to 2.05 (2011). In 2021 the total abundance was 1,292 individuals among 24 taxa and the EBI was 1.84 (Table III- G3). ii. Long Creek Downstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance within downstream Long Creek by SIMPROF revealed slight variation of statistical significance between years among four distinct clusters (Figure III-G10 b). Cluster A consisted of only one year (2012), B consisted of three years (2016, 2019, and 2021), C consisted of five years (2011, 2013-2015, and 2018) and D consisted of 2017 and 2020. Comparison of interannual variability by SIMPER indicated that the clusters A and D were least similar with a 34 percent similarity. This difference was predominantly driven by higher abundances of Americamysis almyra in A and an absence of Nematoda species in D. Sweeps within downstream Long Creek have not contained any new dominants since 2014, but usually contained species of Gammarus (nine of the 11 years had both G. tigrinus and G. mucronatus as dominants) and species of Apocorophium (almost every year) (Table III-G2). Species richness ranged from 17 (2020) to 37 taxa (2015), total abundance ranged from 530 (2020) to 1,566 individuals (2017), and EBI scores ranged from 1.86 (2021) to 2.12 (2016). In 2021, total abundance was 672 individuals among 26 taxa and the EBI score was the lowest to date (1.86) (Table III-G3). Long Creek Upstream Ponar Grabs According to SIMPROF, there were no clusters among the 11 years of ponar grabs for upstream Long Creek. Ponar grabs within upstream Long Creek usually contained Chironomus decorus (eight out of 11 years) and species of Gammarus (nine out of 11 years) as dominants (two of the eight years had both G. tigrinus and G. mucronatus as dominants), with Ampphicteis floridus (two out of 11 years) a less frequent dominant. Tanypus neopunctipennis was a new dominant species in 2021 (Table III-G4). Species richness ranged from 8 (2021) to 32 taxa (2018) and total abundance ranged from 86 (2016) to 941 individuals (2013). In 2021, there were 510 individuals among the lowest species richness to date (8 taxa), a Shannon -Wiener diversity score of 0.52 (previous range: 1.11 — 2.56) and an EBI score of 1.18 (previous range 1.22 — 2.31) (Table III-G5). iv. Long Creek Downstream Ponar Grabs According to SIMPROF, there were no clusters among the 10 years of ponar grabs for downstream Long Creek. Ponar grabs within downstream Long Creek usually contained Chironomus decorus (six out of 11 years) and Streblospio benedicti (eight out of 11 years as dominants. Like Long upstream, Tanypus neopunctipennis was a new dominant in 2021 (Table III-G4). Species richness ranged from 9 (2020) to 22 taxa (2018) and total abundance ranged III-G-23 from 70 (2020) to 941 individuals (2015). In 2021, total abundance was 511 individuals among 10 taxa. Shannon -Wiener diversity score was the lowest to date with 0.54 (previous range: 0.94- 2.66) and the EBI score was 1.06 (range: 1.27-2.03) (Table III-G5). Muddy Creek (1998-2005; 2007-2021) The 2021 benthos samples for Muddy Creek represented the 23rd year of benthic macroinvertebrate collections and as the only early control creek, it has the longest data set among all creeks in the study for benthos, fish, and sediment metals. For sweeps to date the number of dominants identified to species was eight for upstream and 11 for downstream; among those species none was found only upstream and three were only downstream (Table III-G2). Community structure metrics for upstream and downstream sweeps by year are in Table III-G3. For ponars to date the number of dominants identified to species was 18 for upstream and 17 for downstream; among those species four were found only upstream and three only downstream (Table III-G4). Community structure metrics for upstream and downstream ponars by year in Muddy Creek are in Table III-G5. i. Muddy Creek Upstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance from upstream Muddy Creek by SIMPROF revealed statistically significant variation between years among six distinct clusters (Figure III-G11 a). Cluster A contained 1998, B contained four years (1999-2001, and 2004), C contained 2002, D contained 2019, E contained seven years (2005, 2007, 2015-2018, and 2021), and F contained nine years (2003, 2008-2014, and 2020). Clusters that had the lowest percent similarity were A and C (37.3) predominantly due to higher abundances of species of Apedilum and Polydora cornuta in C. Sweep data revealed no new taxa for upstream Muddy Creek dominants since 2012. Dominants over the years usually contained species of Littoridinops (22 out of 23 years), Gammarus, and Apocorophium (both 15 out of 23 years) (Table III-G2). Species richness ranged between 23 (2010) and 40 taxa (2019), total abundance ranged from 602 (2012) to 2,521 individuals (1998), and EBI scores ranged from 1.67 (1998) to 2.42 (2008). In 2021 abundance was 1,314 individuals among 24 taxa and EBI was 2.00 (Table III-G3). ii. Muddy Creek Downstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance from downstream Muddy Creek by SIMPROF revealed statistically significant variation between years among eight distinct clusters (Figure III-G11 b). Cluster A contained three years (2002, 2008, and 2009), B contained 1998, C contained two years (2012 and 2020), D contained year 1999, E contained eight years (2011 and 2013), F contained four years (2000, 2003, 2007, and 2010), G contained three years (2001, 2004, and 2005), and H contained the remainder of the years (2014-2019, and 2021). Sweeps within downstream Muddy Creek usually contained Littoridinops species (19 out of 23 years), Apocorophium species (17 out of 23 years), and Gammarus species (20 out of 23 years) as dominants; no new dominant species have appeared since 2012 (Table III-G2). Species richness ranged from 23 (2021) to 50 taxa (1998), total abundance ranged from 532 (2012) to 3,062 individuals (1998), and EBI scores ranged from 1.77 (2001) to 2.47 (2008). In 2021, there were 900 individuals among the lowest species richness to date (23 taxa), and an EBI score of 2.01 (Table III-G3). III-G-24 iii. Muddy Creek Upstream Ponar Grabs Multivariate cluster analysis of benthic ponar grabs taxa richness and abundance from upstream Muddy Creek by a similarity profile test (SIMPROF) revealed statistically significant variation between years among four distinct clusters (Figure III-G11 c). Cluster A contained two years (2012 and 2014), B contained two years (2017 and 2020), C contained two years (1998 and 1999), and D contained 17 years (2000-2005, 20072011, 2013, 2015, 2016, 2018, 2019, and 2021). Ponar grab data revealed that upstream Muddy Creek most often contained Chironomus decorus (17 out of 23 years) as a dominant, with Streblospio benedicti (eight out of 23 years), and species of Gammarus (seven out of 23 years) less frequently; 2020 was the last year a new dominant species appeared (Table III-G4). Species richness ranged from six (2001) to 21 (1998) and total abundance ranged from 35 individuals (2008) to 1,191 individuals (1998). In 2021, there were 256 individuals among 10 taxa, Shannon -Weiner score was 0.71 (previous range: 0.82 — 2.37), and EBI score was 1.20 (range: 1.05 — 2.05) (Table III-G5). iv. Muddy Creek Downstream Ponar Grabs Multivariate cluster analysis of benthic ponar grabs taxa richness and abundance from downstream Muddy Creek by SIMPROF revealed variation of statistical significance between years among five distinct clusters (Figure III-G11 d). Cluster A contained 1998, B contained two years (2017 and 2020), C contained 2008, D contained 2013, and E contained the remainder years (1999 — 2005, 2007, 2009 — 2012, and 2014 — 2016, 2018-2019, and 2021). Ponar grabs in downstream Muddy Creek usually contained Chironomus decorus (18 out of the 23 years) with Gammarus tigrinus (six out of 23 years) and Streblospio benedicti (nine out of 23 years) appearing less frequently; one new dominant species was present in 2021, Tanypus neopunctipennis (Table III-G4). Species richness ranged from 12 (2003 and 2008) to 26 taxa (2009) and total abundance ranged from 61 (2020) to 1,329 individuals (2011). In 2021, the total abundance was 498 individuals among 14 taxa, a Shannon -Wiener diversity score of 0.63 (previous range: 1.02 — 2.54), and an EBI score of 1.12 (previous range: 1.24 — 2.02) (Table III-G5). DCUT19 (2013-2021) The 2021 benthos samples for DCUT19 represented the eighth consecutive year of Mod Alt L benthic macroinvertebrate collections. For the sweeps to date the number of dominants identified to species was eight for upstream and nine for downstream; among those species one was found only upstream and one only downstream (Table III-G2). Community structure metrics for upstream and downstream sweeps by year are in Table III-G3. For the ponars to date the number of dominants identified to species was seven for upstream and nine for downstream; among those species one was found only upstream and three only downstream (Table III-G4). Community structure metrics for upstream and downstream ponars by year are in Table III-G5. i. DCUT19 Upstream Sweeps Multivariate cluster analysis of benthic sweeps taxa richness and abundance within upstream DCUT19 by SIMPROF showed no statistical significance between study years. Sweep data revealed that upstream DCUT19 contained species of Littoridinops each year as a dominant with Goeldichironomus devineyae (two out of eight years) III-G-25 and species of Tanytarsus (four out of eight years) less often; no new species appeared for the first time as a dominant in 2021 (Table III-G2). Species richness ranged from 23 (2013) to 33 (2016), total abundance ranged from 674 individuals (2021) to 2,011 individuals (2014), and EBI scores ranged from 1.54 (2019) to 2.00 (2018). In 2021, the total abundance was the lowest to date (674 individuals) among 24 taxa with an EBI of 1.84 (Table III-G3). ii. DCUT19 Downstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance from downstream DCUT19 by SIMPROF revealed statistically significant variation between years among two distinct clusters (Figure III-G12). Cluster A only had 2021, while cluster B had the remaining years (2013-2020). Sweeps collected in downstream DCUT19 contained Littoridinops species every year as a dominant and often had Gammarus tigrinus (seven out of eight years), and Hargeria rapax (four out of eight years) as other dominants. There were three new dominant species present in 2021 (Table III-G2). Species richness ranged from 23 (2021) to 35 (2014), total abundance ranged from 456 individuals (2021) to 2,471 individuals (2013), and EBI scores ranged from 1.74 (2016) to 1.99 (2018). In 2021, total abundance was the lowest to date (456 individuals) among the lowest species richness (23 taxa), and an EBI score of 1.88 (Table III-G3). DCUT19 Upstream Ponar Grabs According to SIMPROF, there were no clusters among the eight years of ponar grabs from upstream DCUT19. Ponar grabs revealed that upstream DCUT19 contained species of Littoridinops each year and often contained Chironomus decorus (four out of eight years) as dominants; Dicrotendipes nervosus was a new dominant species in 2021 (Table III-G4). Species richness ranged from 13 (2015 and 2018) to 21 (2013) and total abundance ranged from 170 individuals (2015) to 1,169 individuals (2016). In 2021, total abundance was 730 individuals among 15 taxa, Shannon -Wiener diversity score was 1.99 (range: 1.67 - 2.49), and EBI score was 1.63 (previous range 1.71 - 2.12) (Table III-G5). iv. DCUT19 Downstream Ponar Grabs According to SIMPROF, there were no clusters among the seven years of ponar grabs from downstream DCUT19. Ponar grabs within downstream DCUT19 contained Gammarus tigrinus every year and often contained Amphicteis floridus (five out of eight years) or Hargeria rapax (five out of eight years) as dominants. Dicrotendipes nervosus was a new dominant species in 2021 (Table III-G4). Species richness ranged from 13 (2019) to 27 (2015) and total abundance ranged from 636 individuals (2018) to 2,641 individuals (2017). In 2021, total abundance was 2,040 individuals among 19 taxa, Shannon -Wiener diversity score was 2.13 (range: 1.25 - 2.74), and EBI score was 1.71 (range 1.71 - 2.03) (Table III-G5). Duck Creek (2011-2021) The 2021 benthos samples for Duck Creek represented the 11th consecutive year of benthic macroinvertebrate collections. For the sweeps to date the number of dominants identified to species was seven for upstream and 10 for downstream; among those species two were found only upstream and five were found only downstream (Table III-G2). Community structure metrics for upstream and downstream sweeps by year are in Table III-G3. For the downstream ponars to date the number of dominants identified to species was six III-G-26 upstream and 15 downstream; among those species one was found only upstream and 10 only downstream (Table III-G4). Community structure metrics for upstream and downstream ponars by year are in Table III-G5. i. Duck Creek Upstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance within upstream Duck Creek by SIMPROF revealed slight statistically significant variation between years among three distinct clusters (Figure III-G13 a). Cluster A contained 2019 and 2021, B contained four years (2015-2018), and C contained five years (2011-2014 and 2020). Comparison of interannual variability between the clusters by SIMPER indicated similarity ranged from 50.4 to 54.4 percent. Clusters A and C were the least similar (50.4 percent similarity) with differences predominantly driven by the absence of Dero species, Pristina species, and Kiefferulus species in C. Sweeps in upstream Duck Creek almost always contained Gammarus tigrinus (10 out of 11 years) and sometimes contained Dicrotendipes nervosus (three out of 11 years) and Lirttordinops tenuipes (three out of 11 years) as dominants; no new dominant species occurred in 2021 (Table III-G2). Species richness ranged from 19 (2021) to 34 taxa (2017), total abundance ranged from 627 (2013) to 1,956 individuals (2015), and EBI scores ranged from 1.50 (2019) to 1.92 (2013 and 2020). In 2021, total abundance was 1,357 individuals among the lowest species richness to date of 19 taxa and EBI score was 1.64 (Table III-G3). ii. Duck Creek Downstream Sweeps Multivariate cluster analysis of benthic sweep taxa richness and abundance within downstream Duck Creek by SIMPROF revealed slight statistically significant variation between years among four distinct clusters (Figure III-G13 b). Cluster A and B contained one year each (2021 and 2020, respectively), C contained three years (2015-2017 and 2019), D contained five years (2011-2014 and 2018). Comparison of interannual variability between the clusters by SIMPER indicated percent similarity ranged from 45.1 to 61.3. Clusters A and D were less similar due largely to higher abundance of Hargeria rapax, Amphicteis floridus, and species of Chironomus in D. Sweeps in downstream Duck Creek usually contained Gammarus tigrinus (10 out of 11 years) and Littoridinops species (nine out of 11 years) as dominants, while Paleomonetes pugio was a dominant for the third year in a row in 2021 (first appearance in 2019) (Table III-G2). Species richness ranged from 20 (2016) to 32 taxa (2013 and 2018), total abundance ranged from 574 (2021) to 2,172 individuals (2014), and EBI scores ranged from 1.89 (2018) to 2.13 (2016). In 2021, total abundance was the lowest to date (574 individuals) among 24 taxa and an EBI score of 2.03 (Table III-G3). iii. Duck Creek Upstream Ponar Grabs Multivariate cluster analysis of benthic ponar grabs taxa richness and abundance within upstream Duck Creek by a similarity profile test (SIMPROF) revealed slight statistically significant variation between years between three distinct clusters (Figure III-G13 c). Cluster A and B had one year each (2019 and 2021, respectively) and C contained the remainder (2011-2018 and 2020). Comparison of interannual variability between the clusters by SIMPER indicated clusters A and B were 30.2 percent similar with differences predominately due to the absence of Amphicteis floridus and greater abundance of species of Candonidae in A. Ponar grabs in upstream Duck Creek consistently contained Gammarus tigrinus (nine out of 11 years), while Amphicteis floridus (eight out of 11 years) was III-G-27 frequently its co -dominant species; 2016 was the last year a new dominant appeared (Table III- G4). Species richness ranged from eight (2018) to 19 taxa (2013 and 2016) and total abundance ranged from 122 (2021) to 1,944 individuals (2014). In 2021, total abundance was the lowest to date (122 individuals) among 13 taxa, the Shannon -Wiener diversity score was 2.27 (previous range: 1.34 — 2.20), and the EBI score was 1.64 (range: 1.54 — 2.01) (Table III-G5). iv. Duck Creek Downstream Ponar Grabs According to SIMPROF, there were no clusters among the nine years of ponar grabs for downstream Duck Creek. Ponar grabs in downstream Duck Creek frequently contained Chironomus decorus (eight out of 11 years) as dominant species often with Mediomastus ambiseta (four out of 11 years), Gammarus tigrinus (three out of 11 years), and Amphicteis floridus (two out of 11 years) less frequently; no new dominant species appeared in 2021 (Table III-G4). Species richness ranged from 11 (2019) to 25 (2017) and total abundance ranged from 147 individuals (2016) to 1,206 individuals (2011). In 2021, total abundance was 285 individuals among 14 taxa, Shannon -Wiener diversity score was 1.54 (range: 0.83 — 2.20), and the EBI score was 1.35 (range: 1.27 — 2.25) (Table III-G5). 2.0 Ponar Grabs Benthic Feeding Guild and Trophic Level Results Each species ever collected in a ponar grab was assigned a number between 0 and 3 for each of the four trophic level categories (herbivore, detritivore, carnivore, and parasite) and each of the six functional feeding guild categories (gatherer/collector, filterer/collector, scraper, predator, grazer, and shredder). A '0' indicated that the species had no affinity to that particular category whereas a '3' indicated that the species had high affinity to that particular category. An advantage to this approach is that a species could be assigned nonzero values in multiple categories, which addresses species that are omnivorous (e.g., herbivore and carnivore), or species that display multiple or facultative feeding modes (e.g., predator and gatherer/collector). Omnivory and multiple feeding modes are extremely common among benthic macroinvertebrates. The technique of fuzzy correspondence analysis (FCA) allowed examination of the list of species designations for differentiation. An FCA is similar to an ordinary correspondence analysis except that species can have membership in multiple categories. The outputs of an FCA are standardized, normally distributed scores for a certain number of axes for each species. Each axis represents some type of differentiation of the categories. From year to year, an axis number and the categories along that axis number may change as far as the loadings are determined. Six axes were produced which differentiated the trophic level categories and functional feeding guild categories. Thus, each species was described by a separate score for six different axes, each describing a different combination of trophic level and/or functional feeding guild (refer to Section II-C for a description of the guild and trophic level distribution across the six axes and a depiction of the loadings). To characterize the trophic level and functional feeding guild of the benthic macroinvertebrate community of each creek, each species' FCA score on each of the six axes was multiplied by its relative abundance for each ponar grab conducted in the creek. Relative abundance was computed as the abundance of a particular species divided by the total abundance of the sample. Species with an abundance of 0 were given a relative abundance of 0. A sum of the FCA scores weighted by the relative abundance of each species was obtained. This process was repeated for each of the six axes from the FCA. Thus, the benthic III-G-28 macroinvertebrate community of every ponar grab across all creeks, years, locations (upstream vs. downstream), and sample (1 - 5) is described by this summed value for each of the six FCA axes. These summed weighted FCA scores were used in subsequent statistical analyses. Temporal depictions within the six axes and cluster dendrograms based on the FCA scores are in Figures III-G14 — G31 and described in detail below. The arrows above or below zero on the right edge of the temporal depictions of the six axes are the same on each figure and indicate the relationships among the major trophic levels and/or feeding guilds for that axis. Values relative to zero (positive or negative designations as shown on Figure II-C21 a - f) are important to note for interpretation of the benthic community relationships in the Section III-G upstream/downstream axis figures; e.g., an "increase" in a particular year fora specific guild could appear as a rise above zero on one axis and a dip below zero on another, depending on the negative or positive designation for that specific trophic level or guild across all years (as shown in Figure II-C21 a - f). Gatherer/collector and predator were consistently close to the axes' origins and therefore less distinctly differentiated. a. Post -Mod Alt L Creeks Guild Data from Ponar Grabs i. Jacks Creek Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for upstream ponar grabs of Jacks Creek by SIMPROF revealed slight variation of statistical significance between years among seven distinct clusters (Figure III-G14 a). Cluster A contained 2012, B contained five years (2002-2004, 2013, and 2020), C contained two years (2000 and 2021), D contained four years (2001, 2005, 2011, and 2015), E contained 1998, F contained two years (2014 and 2016), and G contained five years (1999, 2017-2019). The six post -Mod Alt L years split among four of the groups with pre -Mod Alt L years. The upstream ponar grab macroinvertebrate community data were mainly composed of the carnivore trophic level in the three earliest years (1998 — 2000) and in 2021, but mostly composed of the herbivore and detritivore trophic levels (Figure III-G14 b). Feeding guilds have been variable over the years, but in 2021, there was an increase in shredder. Comparison of interannual variability between pre- and post -Mod Alt L ponar grab taxa FCA scores in upstream Jacks Creek by SIMPER revealed that differences were predominantly due to slightly less herbivore and detritivore in the post -Mod Alt L years. However, comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities of upstream Jacks Creek by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for Jacks Creek upstream stations were compared to the corresponding years and stations for Muddy Creek (control creek containing long-term data from 1998 only and Little Creek (closest control creek with data from 2011)). Separate mixed -model analysis of variances (ANOVAs) for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed no significant Creek: Mod Alt L Status interaction for the comparison of upstream Jacks Creek to Muddy Creek (Figure III-G14 c). The lack of a significant interaction indicates that changes in guild structure between pre- and post -Mod Alt L years did not differ between impact and control creeks. III-G-29 ii. Jacks Creek Downstream Guilds/Trophic Levels According to SIMPROF, there were no clusters among FCA scores for ponar grabs. Herbivore and detritivore trophic levels consistently dominated the downstream benthic macroinvertebrate community of Jacks Creek. However, herbivore and detritivore trophic guilds trended closer to zero in 1999, 2000, and 2020 (Figure III-G14 b). Predominance of different functional feeding guilds did slightly vary, mostly between scraper, grazer, and parasite; although shredder trended closer to zero from 2015-2017, and 2020 on Axis 4 (Figure III-G14 b). Filter -collector was predominant from 2011-2019; although there was a trend towards zero in 2013, 2015, and 2019. Comparison of interannual variability between pre- and post -Mod Alt L downstream ponar grab taxa FCA scores within Jacks Creek by SIMPER revealed that differences were driven predominantly by more scraper in the post -Mod Alt L years. However, comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities of downstream Jacks Creek by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for Jacks Creek downstream ponar samples were compared to the corresponding years and samples for the control creeks Muddy and Little. Separate mixed -model ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed no significant Creek: Mod Alt L Status interaction for the comparison between Jacks Creek and Little or Muddy creeks (Figure III-G14 c). The lack of a significant interaction indicates that changes in guild structure between pre- and post -Mod Alt L years did not differ between impact and control creeks. N. Jacobs Creek Upstream Guilds/Trophic Levels According to SIMPROF, there were no clusters among FCA scores for ponar grabs. Ponar grab data revealed that the benthic macroinvertebrate community in 2021 upstream Jacobs Creek was mostly composed of carnivore and parasite trophic levels, which has not occurred yet in Jacobs Creek (Figure III-G15 b). Guilds and trophic levels have not varied substantially over the ten years; however, there was some variability among each ponar grab sample in 2013, 2016, 2017, and 2020. Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores within upstream Jacobs Creek by SIMPER revealed that differences were predominantly due to slightly more shredder and scraper for the post -Mod Alt L years. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities of upstream Jacobs Creek by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for Jacobs Creek upstream stations were compared to the corresponding years for PA2 (closest control creek) and Long Creek (control creek across South Creek from Jacobs Creek). Separate ANOVAs for each FCA axis, with Creek Status (Impact/Control) as fixed effects and ponar subsample as a random effect, revealed no significant Creek: Mod Alt L Status interaction for the comparisons. The lack of a significant interaction indicates that changes in guild structure III-G-30 between pre- and post -Mod Alt L years did not differ between impact and control creeks (Figure III-G15). iv. Jacobs Creek Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for downstream ponar grabs of Jacobs Creek by SIMPROF revealed slight variation of statistical significance between years among two distinct clusters (Figure III-G15 a). Cluster A consisted of 2014 (the first year post -Mod Alt L) and 2020 and B contained a mixture of pre- and post -Mod Alt L years. Trophic and functional feeding guilds drove differences between clusters A and B predominately by more scraper and grazer in A. Downstream Jacobs Creek in 2021 was mostly composed of filter - collector functional feeding guild (Axis 3) (Figure III-G15 b). This composition has varied over the 10 years, becoming more abundant in 2015-2019, followed by a composition closer to zero until 2021 (Axis 3). Scraper, grazer, and parasite increased from 2016-2018 before shredder started to increase. Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores within downstream Jacobs Creek by SIMPER revealed that differences were predominantly due to slightly more shredder, grazer, and parasite for the post - Mod Alt L years. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities of downstream Jacobs Creek by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for Jacobs Creek downstream were compared to the corresponding years for PA2 and Long Creek. Separate mixed -model ANOVAs for each FCA axis with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect revealed no significant interactions. The lack of a significant interaction indicates that changes in guild structure between pre- and post - Mod Alt L years did not differ between impact and control creeks (Figure III-G15 c). v. Drinkwater Creek Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream Drinkwater Creek by SIMPROF revealed slight variation of statistical significance between years among three distinct clusters (Figure III-G16 a). Cluster A consisted of two post - Mod Alt L years (2013 and 2015), B contained two years (2011 and 2017), and C contained the rest of the years (2012, 2014, 2016, 2018-2021). Ponar grab data revealed that the benthic macroinvertebrate community in upstream Drinkwater Creek was mostly composed of herbivore and detritivore trophic levels; however, herbivore and detritivore trended toward zero in 2020 and 2021. Except for slight changes in shredder, scraper, or grazer, the feeding guild relationships in the upstream community did not vary much over the years (Figure III-G16 b). Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores within upstream Drinkwater Creek by SIMPER revealed that differences were predominantly due to slightly more shredder and scraper post -Mod Alt L. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities in upstream Drinkwater Creek by means of ANOSIM detected no spatial differences of statistical significance. III-G-31 Differences in guild composition between pre- and post -Mod Alt L years for Drinkwater Creek upstream were compared to the corresponding years for PA2 (closest control creek) and Long Creek (control creek across South Creek from Drinkwater Creek). Separate ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed significant Creek: Mod Alt L Status interactions for the comparison between Drinkwater Creek and PA2 or between Drinkwater Creek and Long Creek (Figure III-G17). The lack of a significant interaction indicates that changes in guild structure between pre- and post -Mod Alt L years did not differ between creeks. vi. Drinkwater Creek Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream Drinkwater Creek by SIMPROF revealed slight variation of statistical significance between years among two distinct clusters (Figure III-G16 c). Cluster A consisted of one post - Mod Alt L year (2014) and B contained the remaining years (2011-2013, 2015-2021). Differences between cluster A and B was predominantly driven by slightly more scraper in A. Similar to upstream, the downstream benthic macroinvertebrate community was mostly composed of herbivore and detritivore trophic levels; Axis 3 differentiation from zero decreased since 2012, an indication that composition of shredder and grazer more closely resembled the other guilds after 2012 (Figure III-G16 b). Filterer/collector was the predominant functional feeding guild, except in 2014, 2016, and 2017. Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores within downstream Drinkwater Creek by SIMPER revealed differences driven predominantly by more scrapers for the post -Mod Alt L years. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities in downstream Drinkwater Creek by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for Drinkwater Creek downstream were compared to the corresponding years for PA2 (closest control creek) and Long Creek (control creek across South Creek from Drinkwater Creek). Separate ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed one significant Creek: Mod Alt L Status interaction for the comparison between Drinkwater Creek and PA2 (Axis 1: F = 7.54, P = 0.01); herbivore and detritivore trophic levels were greater in post -Mod Alt L years of Drinkwater Creek than in PA2 (Figure III-G17). vii. Tooley Creek Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream Tooley Creek by SIMPROF revealed slight variation of statistical significance between years among two distinct clusters (Figure III-G18 a). Cluster A contained 2020, while cluster B contained the remaining years. Ponar grab data revealed that the benthic macroinvertebrate community in upstream Tooley Creek was mostly composed of herbivore and detritivore trophic levels, with a temporary increase in carnivore and parasite in 1999 and 2000. Filter -collector was a predominant guild from 2010-2016, 2018, and 2021, with a slight decrease in 2013-2015 (Axis 2). Scraper increased in relative abundance from 2015-2020 (Axis 6) (Figure III-G18 b). III-G-32 Comparison of interannual variability between pre- and post -Mod Alt L ponar grab taxa FCA scores within upstream Tooley Creek by SIMPER revealed that differences were driven predominantly by slightly more scraper and shredder for the post -Mod Alt L years. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities in upstream Tooley Creek by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for Tooley Creek upstream stations were compared to the corresponding years and stations for Muddy Creek (control creek with long-term data from 1998), PA2 (control creek just south of Tooley Creek with data since 2011), and Long Creek (control creek across South Creek from Tooley Creek with data since 2011). Separate ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed no significant Creek: Mod Alt L Status interactions for the comparison between Tooley Creek and Muddy Creek, Tooley Creek and PA2, or Tooley Creek and Long Creek (Figure III-G19). The lack of a significant interaction indicates that changes in guild structure between pre- and post -Mod Alt L years did not differ between impact and control creeks. viii. Tooley Creek Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within downstream Tooley Creek by SIMPROF revealed slight variation of statistical significance between years among six distinct clusters (Figure III-G18 c). Cluster A contained one post -Mod Alt L year (2016), B contained two early years (1998 and 2000), C contained two post -Mod Alt L years (2014 and 2018), D contained 2020, E contained 1999, and F contained eight years (2001, 2010-2013, 2015, 2017, 2019, and 2021) (Figure III-G18 c). Clusters B and E contained all pre - Mod Alt L years, while clusters A, C, and D contained all post -Mod Alt L years (Figure III-G18 c). Similar to upstream ponar grabs, the downstream benthic macroinvertebrate community of Tooley Creek was composed mostly of herbivore and detritivore, with a slight decrease only in 1999 (Figure III-G18 b). Filter/collector varied in earlier years but was the predominant functional feeding guild from 2010-2018 (Axis 2). Scraper, grazer, and parasite increased sharply in 2016 (Axis 4), but slowly declined from 2017-2021. There was variability in guild composition among ponar samples in 2016 (Figure III-G18 b). Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores from downstream Tooley Creek by SIMPER revealed that differences were driven predominantly by slightly more grazer, scraper, and parasite for the post -Mod Alt L years. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities in downstream Tooley Creek by means of ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for Tooley Creek downstream were compared to the corresponding years and stations for Muddy and Long creeks and PA2. Separate mixed -model ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed a significant Creek: Mod Alt L Status interaction for the Tooley/Muddy comparison (Axis 1: F = 6.31, P = 0.02) and Tooley/Long (Axis 1: F = 7.04, P = 0.02), but none for Tooley/PA2 comparison (Figure III-G19). Comparisons revealed greater abundances of herbivore and detritivore trophic level for Tooley Creek than both Muddy Creek and Long Creek post -Mod Alt L. III-G-33 ix. Huddles Cut Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream Huddles Cut by SIMPROF revealed slight variation of statistical significance between years among six distinct clusters (Figure III-G20 a). Cluster A contained three post -Mod Alt L years (2010, 2014, and 2018), B and C both contained one year (2016 and 2021, respectively), D contained two pre -Mod Alt L years (2000 and 2019), E contained two years (1999 and 2009), and F contained nine years (2001, 2007, 2008, 2011-2013, 2015, 2017, and 2020) (Figure III-G20 a). Ponar grab data revealed that the benthic macroinvertebrate community in upstream Huddles Cut was mostly composed of herbivore and detritivore, until 2021 where carnivore and parasite increased. The other functional feeding guilds fluctuated around zero/the axis across the years with the greatest variation in 2010 (Figure III-G20 b). Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores from upstream Huddles Cut by SIMPER revealed that differences were driven predominantly by slightly more filter/collector for the post -Mod Alt L years. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities in upstream Huddles Cut by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for Huddles Cut upstream stations were compared to the corresponding years for Muddy Creek (control creek with long-term data from 1998). Separate mixed -model ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed one significant Creek: Mod Alt L Status interaction for the comparison between Huddles Cut and Muddy Creek (Axis 1: F = 4.68, P = 0.04) (Figure III-G21). There were slightly less herbivore and detritivore in Huddles pre -Mod Alt L compared to Muddy Creek. x. Huddles Cut Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within downstream Huddles Cut by SIMPROF revealed slight variation of significance between years among three distinct clusters (Figure III-G20 c). Cluster A contained four years (2008, 2011, 2014, and 2015), B contained five years (2000, 2009, 2010, 2018, and 2019), and C contained nine years (1999, 2001, 2007, 2012, 2013, 2016, 2017, 2020, and 2021). Each cluster contained a mix of pre- and post -Mod Alt L years. Unlike upstream, the benthic macroinvertebrate community of downstream ponar grabs of Huddles Cut was not dominated by herbivore and detritivore every year but was also dominated by parasite and/or carnivore for several years (2008, 2011, 2014, and 2015) (Figure III-G20 b). The dominant functional feeding guilds varied little in previous years, except for an increase in scraper, grazer, and parasite in 2001 and 2012 (Axis 4) and an increase in 2020 of filter -collector (Axis 2). Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores within downstream Huddles Cut by SIMPER revealed that differences were driven predominantly by slightly less shredder and scraper for the post -Mod Alt L years. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities in downstream Huddles Cut ponar grabs by ANOSIM detected no spatial differences of statistical significance. III-G-34 Differences in guild composition between pre- and post -Mod Alt L years for Huddles Cut downstream were compared to the corresponding years for Muddy Creek. Separate mixed -model ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed no significant Creek: Mod Alt L Status interactions for the comparison between Huddles Cut and Muddy Creek (Figure III-G21). The lack of a significant interaction indicates that changes in guild structure between pre- and post -Mod Alt L years did not differ between creeks. xi. Porter Creek Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream Porter Creek by SIMPROF revealed slight variation of significance between years among four distinct clusters (Figure III-G22 a). Cluster A contained one pre -Mod Alt L year (2013), B contained three post -Mod Alt L years (2018-2020), C contained four years (2011, 2012, 2014, and 2021), and D contained three years (2015-2017). Ponar grab data revealed that the upstream Porter Creek benthic macroinvertebrate community was mostly composed of herbivore and detritivore trophic levels and filter -collector functional feeding guild (Figure III-G22 b). Functional feeding guilds varied over the years. However, there was a decrease in shredder and scraper from 2014 to 2016 (Axis 3) (Figure III-G22 b). Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores within upstream Porter Creek by SIMPER revealed that differences were driven predominantly by slightly more scraper and shredder post -Mod Alt L (2016-2020). Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities in upstream Porter Creek ponar grabs by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for Porter Creek upstream were compared to the corresponding years for Duck Creek (closest control creek; across the Pamlico River) and Little Creek (control creek in South Creek with similar basin size). Separate mixed -model ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed significant Creek: Mod Alt L Status interactions for the comparison between Porter and Little Creek (Axis 2: F = 7.73, P = 0.04), but not for Porter Creek and Duck Creek (Figure III-G23). There were slightly less filter/collector in Porter Creek pre -Mod Alt L compared to Little Creek. xii. Porter Creek Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within downstream Porter Creek by SIMPROF revealed slight variation of significance between years among four distinct clusters (Figure III-G22 c). Cluster A contained 2020, B contained six years (2011, 2013, 2015-2018), C contained two years (2012 and 2014), and D contained two years (2019 and 2021). Similar to upstream, the benthic community of the downstream Porter Creek ponar grabs was mostly composed of the herbivore and detritivore trophic level (Figure III-G22 b). Grazer, scraper, and shredder were the predominant functional feeding guild in 2019 and 2020, while filter/collector was predominant from 2011-2018. Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores within downstream Porter Creek by SIMPER revealed that III-G-35 differences were driven predominantly by slightly more scraper post -Mod Alt L. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities in upstream Porter Creek ponar grabs by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for Porter Creek downstream were compared to the corresponding years and stations for Duck and Little creeks. Separate mixed -model ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed no significant Creek:Mod Alt L Status interactions for the comparison between either Porter Creek and Duck Creek or between Porter Creek and Little Creek (Figure III-G23). xiii. DCUT11 Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for upstream ponar grabs of DCUT11 by SIMPROF revealed slight variation of statistical significance between years among five distinct clusters (Figure III-G24 a). Clusters A, B, and C each contained one pre -Mod Alt L year (2014, 2016, and 2013, respectively), D contained three years (2017-2019), and E contained three years (2015, 2020, and 2021) (Figure III-G24 a). The benthic macroinvertebrate community in upstream ponar grabs of DCUT11 was mostly composed of herbivore and detritivore trophic level (Figure III-G24 b). Grazer, scraper and shredder (Axis 2), scraper, grazer, and parasite (Axis 4), and scraper (Axis 6) were predominant guilds in 2015 and 2020-2021 but trended near zero in other years (Figure III-G24 b). Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores within upstream DCUT11 by SIMPER revealed differences were driven predominantly by more shredder and scraper post -Mod Alt L. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities in upstream DCUT11 ponar grabs by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for DCUT11 downstream were compared to the corresponding years and stations for Duck and DCUT19. Separate mixed -model ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed no significant Creek: Mod Alt L Status interactions for the comparison between DCUT11 and Duck Creek (Figure III- G25). xiv. DCUT11 Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for downstream ponar grabs of DCUT11 by SIMPROF revealed slight variation of statistical significance between years among three distinct clusters (Figure III-G24 c). Cluster A consisted of two years (2013 and 2018), B contained three years (2017, 2019, and 2021), and C contained four years (2014- 2016 and 2020) (Figure III-G24 c). Similar to upstream, the benthic macroinvertebrate community of downstream ponar grabs of DCUT11 was mostly composed of the herbivore and detritivore trophic level (Figure III-G24 b). Filterer/collector was the predominant functional feeding guild, particularly in 2013, 2018, and 2020 (Figure III-G24 b). III-G-36 Comparison of interannual variability between pre- and post -Mod Alt L ponar taxa FCA scores within downstream DCUT11 by similarity percentages (SIMPER) revealed differences were driven predominantly by more herbivore and detritivore post -Mod Alt L. Comparison of interannual variability between pre- and post -Mod Alt L guild and trophic level composition of benthic macroinvertebrate communities in downstream DCUT11 ponar grabs by ANOSIM detected no spatial differences of statistical significance. Differences in guild composition between pre- and post -Mod Alt L years for DCUT11 downstream were compared to the corresponding years and stations for Duck and DCUT19. Separate mixed -model ANOVAs for each FCA axis, with Creek and Mod Alt L Status as fixed effects and ponar subsample as a random effect, revealed no significant Creek: Mod Alt L Status interactions for the comparison between DCUT11 and Duck Creek (Figure III- G25). b. Control Creeks Guild/Trophic Level Data from Ponar Grabs i. Little Creek Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for the ponar grabs within upstream Little Creek by SIMPROF revealed slight variation of statistical significance between years among three distinct clusters (Figure III-G26 a). Cluster A contained 2011, B contained 2021, and C contained the other eight years (2012-2020) (Figure III-G26 a). Ponar grab data revealed that the benthic macroinvertebrate community within upstream Little Creek was composed mostly of herbivore and detritivore trophic levels (Figure III-G26 b). The dominant functional feeding guilds varied little over 11 years, except for the increase in grazer, scraper and shredder (Axis 2), scraper, grazer, and parasite (Axis 4), and scraper (Axis 6) in 2011 (Figure III-G26 b). ii. Little Creek Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for the ponar grabs within downstream Little Creek by SIMPROF revealed slight variation of statistical significance between years among five distinct clusters (Figure III-G26 c). Clusters A, B, and C each contained one year (2013, 2019, and 2021, respectively), D contained five years (2012, 2014, 2015, 2017, and 2018), and E contained three years (2011, 2016, and 2020). Similar to upstream Little Creek, the benthic macroinvertebrate community of downstream Little Creek ponar grabs was composed mostly of herbivore and detritivore trophic levels, which trended to zero in 2021 (Figure III-G26 b). Filter -collector was the dominant functional feeding guild for previous years, but trended closer to zero in 2020, then increased again in 2021. Scraper increased slightly in 2019 but trended towards zero in 2021. PA2 Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream PA2 by SIMPROF revealed slight variation of statistical significance between years among five distinct clusters (Figure III-G27 a). Cluster A contained two years (2017 and 2020), B contained 2021, C contained two years (2015 and 2019), D contained two years (2012 and 2013), and E contained four years (2011, 2014, 2016, and 2018). Ponar grab data revealed that the benthic macroinvertebrate community within upstream PA2 was composed mostly of herbivore and detritivore trophic levels, III-G-37 except in 2021 when there were more parasite and carnivore (Axis 1). There was an increase in scraper in 2015, 2017, 2019-2021 (Axis 5 and 6) (Figure III-G27 b). In 2021 shredder, grazer, and parasite decreased. iv. PA2 Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream PA2 by SIMPROF revealed slight variation of statistical significance between years between two distinct clusters (Figure III-G27 c). Cluster A contained 2020, while B contained the remaining years. Similar to upstream, the benthic macroinvertebrate community within downstream PA2 was mostly composed of herbivore and detritivore trophic levels (Figure III-G27 b). The dominant functional feeding guild was scraper although the guild decreased in 2013 and 2014 (Axis 6). Filter -collector increased in 2016 and 2018, but trended towards more grazer, scraper and shredder in 2019-2021 (Axis 2). v. Long Creek Upstream Guilds/Trophic Levels According to SIMPROF, there were no clusters among FCA scores for upstream ponar grabs of Long Creek. Ponar grab data revealed that the benthic macroinvertebrate community within upstream Long Creek was composed mostly of herbivore and detritivore, but trended closer to zero in 2020 and 2021. The functional feeding guilds varied over the 10 years, with slightly less variation since 2016 (Figure III-G28 a). vi. Long Creek Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within downstream Long Creek by SIMPROF revealed slight variation of statistical significance between years among four distinct clusters (Figure III-G28 b). Cluster A contained two years (2016 and 2019), B contained two years (2017 and 2021), C contained five years (2011-2013, 2015, and 2020), and D contained two years (2014 and 2018). Similar to upstream, the benthic macroinvertebrate community of downstream Long Creek ponar grabs mostly consisted of herbivore and detritivore trophic level (Figure III-G28 a). The dominant functional feeding guild was filter/collector although scraper increased in 2016, 2017, and 2019. vii. Muddy Creek Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream Muddy Creek by SIMPROF revealed slight variation of statistical significance between years among four distinct clusters (Figure III-G29 a). Clusters A and B contained one year each (2010 and 2004, respectively), C contained 11 years (1998, 2003, 2005, 2011-2013, 2015, 2016, 2017, 2018, and 2021), and D contained 10 years (1999-2002, 2007-2009, 2014, 2019, 2020). Ponar grab data revealed that the benthic macroinvertebrate community within upstream Muddy Creek was composed mostly of herbivore and detritivore trophic levels, although parasite and carnivore were abundant in 2000, 2004, and 2010 (Figure III-G29 b). Filterer/collector was dominant functional feeding guild, although grazer, scraper, and shredder increased in some years (2000, 2010, 2014, and 2018-2020). III-G-38 viii. Muddy Creek Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within downstream Muddy Creek by SIMPROF revealed slight variation of statistical significance between years among eight distinct clusters (Figure III-G29 c). Cluster A contained two years (1998 and 2013), B contained 2021, C contained two years (2000 and 2008), D contained 2010, E contained three years (1999, 2019, and 2020), F contained six years (2003, 2007, 2011, 2012, 2014, and 2018), G contained 2004, and H contained seven years (2001, 2002, 2005, 2009, 2015, 2016, and 2017). The benthic macroinvertebrate community within downstream Muddy Creek was composed mostly of herbivore and detritivore trophic levels, but trended toward zero in 2019-2021 (Figure III-G29 b). Similar to upstream, filterer/collector was the predominant functional feeding guild, although some years contained higher abundance of scraper, shredder, and grazer (e.g., 1998 and 2013). ix. DCUT19 Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream DCUT19 by SIMPROF revealed slight variation of statistical significance between years among three distinct clusters (Figure III-G30 a). Cluster A contained 2020, B contained four years (2013, 2015, 2016, and 2021), and C contained four years (2014, 2017, 2018, and 2019). Ponar grab data revealed that the benthic macroinvertebrate community within upstream DCUT19 was composed mostly of herbivore and detritivore trophic levels (Figure III-G30 b). The dominant functional feeding guilds were grazer, scraper, and shredder except in 2014 when filter/collector was dominant. Scraper, grazer, and parasite (Axis 4) and scraper (Axis 6) trended towards zero in 2014, 2017, and 2019, but increased in other years. x. DCUT19 Downstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream DCUT19 by SIMPROF revealed slight variation of statistical significance between years among three distinct clusters (Figure III-G30 c). Cluster A contained two years (2013 and 2020), B contained two years (2015 and 2017), and C contained five years (2014, 2016, 2018, 2019, and 2021). Similar to upstream DCUT19, the benthic macroinvertebrate community within downstream DCUT19 ponar grabs consisted mostly of herbivore and detritivore trophic levels (Figure III-G30 b). Filterer/collector was the most dominant functional feeding guild, especially in 2013. Shredder and scraper increased and decreased over the eight years, but steadily increased from 2018-2020 (Axis 3). xi. Duck Creek Upstream Guilds/Trophic Levels Multivariate cluster analysis of the FCA scores for ponar grabs within upstream Duck Creek by SIMPROF revealed slight variation of statistical significance between years between two distinct clusters (Figure III-G31 a). Cluster A contained nine years (2012, 2014-2021) and B contained two years (2011 and 2013). Ponar grab data revealed that the benthic macroinvertebrate community within upstream Duck Creek was composed mostly of herbivore and detritivore trophic levels, although parasite and carnivore increased in 2013. The dominant functional feeding guilds were grazer, scraper, and shredder, although filter -collector increased in 2012 and 2014 (Figure III-G-39 III-G31 b). xii. Duck Creek Downstream Guilds/Trophic Levels According to SIMPROF, there were no clusters among FCA scores for upstream ponar grabs of Duck Creek. The benthic macroinvertebrate community within downstream Duck Creek ponar grabs trophic levels consistently had high abundance of herbivore and detritivore and had an increase of parasite and carnivore in 2015 and 2016; grazer feeding guild increased in 2013, while scrapers increased slightly in 2017 and 2018 (Figure III-G31 b). 3.0 Summary and Conclusions The most common species encountered for most creeks/years for sweeps were (in order): species of Littoridinops (most often not identified to species, but L. tenuipes when it was identified), species of Apocorophium (most often not identified to species, but either A. lacustre, or A. louisianum when identified), and Gammarus tigrinus. The most common species encountered in most creeks/years for ponars were (in order): Chironomus decorus (when present, almost always one of the two most dominant taxa), Gammarus tigrinus, Amphicteis floridus, species of Littoridinops, species of Apocorophium, Mediomastus ambiseta, Streblospio benedicti, and Macoma balthica. Dissimilarities in abundance between these dominant taxa mostly accounted for differences among creeks/years/stations, along with the occasional presence or absence of less dominant taxa in some creeks/years. Interannual comparisons between pre- and post -Mod Alt L years indicated five significant spatial differences among benthic macroinvertebrates for four creeks with post -Mod Alt L data (Sweeps -upstream Jacobs Creek, downstream Tooley Creek, upstream Huddles Cut, and downstream Porter Creek; Ponar-downstream Tooley Creek). This result suggests that close to 82 percent of the comparisons (23 out of 28) in the impacted creeks showed the benthic macroinvertebrate community of post -Mod Alt L years as similar to that of the pre -Mod Alt L years; thus, it is difficult to discern any mine -related patterns in benthic macroinvertebrate communities. Huddles Cut upstream benthic samples were compared to those of Muddy Creek (the only control creek with data corresponding to Huddles Cut pre -Mod Alt L years) to determine possible reasons for significant differences between pre- and post -Mod Alt L community structure. Muddy Creek benthic communities did not experience a significant difference between corresponding pre- and post -Mod Alt L years, as seen in Huddles Cut upstream. Variability displayed at the Huddles Cut upstream benthic station could be attributed to mine activities, but it could also be related to the sand bar which formed at the mouth of Huddles Cut in 2009 (completely blocked flow in some conditions in portions of that year). The sand bar has remained a persistent feature since then and continues to periodically narrow the channel near the mouth, which restricts exchange of water between Huddles Cut and the Pamlico River. Complete blockage has not been noted since 2009; however, sand continues to be redistributed at the mouth of Huddles Cut, forming a dynamic narrow channel that has inlet -like characteristics in response to the movement of the sand from the bar. Some of the sand from the bar appears to have moved to the beach at the mouth which also periodically enters into the channel and contributes to a narrower inlet/outlet. A confident separation of such non -mine related changes in creek condition from potential mine -related changes is difficult. The benthic data continue to show considerable variation between years. For instance, in 2021, benthic abundance and/or taxa count for 27 out of 40 creek sites were record lows for both ponars and/or sweeps. That is ten more sites than in 2020. These record lows have III-G-40 occurred in downstream sites more than upstream (7 upstream sites vs 12 downstream sites for ponars and 5 upstream sites vs 10 downstream sweeps) with an increase in specific tolerant freshwater species occurring in the downstream sites, such as Chironomus decorus, Goeldichironomus devineyae and Dicrotendipes nervosus. Since these record lows were in both impact and control creeks, it's unlikely these changes in benthic abundance and taxa count were due to mine -related activities. This variation in community structure may be explained by temporal changes in water chemistry, recruitment, predation, and the naturally heterogeneous distribution characteristics of estuarine invertebrates (West 1985). Specifically, since record low abundance and taxa numbers were in downstream sites more than upstream, lower salinity levels and greater volume of Tar River discharge over the past few years could be factors in changes within the benthic community. For example, variation in salinity concentration seems to have driven population changes in several commonly collected stenohaline taxa. Amphicteis floridus and Streblospio benedicti, two species that have a preference for freshwater, have increased in abundance in years where salinity is lower in contrast to Mediomastus ambiseta and Macoma balthica, two species that prefer mesohaline conditions and have increased during years of high salinity. Salinity in the Pamlico River and its tributaries is mostly driven by discharge from the Tar River and changes in sea level (Kimmel 2016); thus, it seems likely that the changes seen in the benthic macroinvertebrate communities of the PCS study creeks are mostly in response to external abiotic conditions. This conclusion corresponds to other studies which demonstrated the importance of abiotic conditions like river discharge on salt marsh communities in Georgia, USA (Wieski and Pennings 2014, Li and Pennings 2016). Temporal variability analysis showed that the macroinvertebrate ponar (upstream/downstream) guilds/trophic levels displayed a strong positive correlation with environmental variables within two creeks (DCUT19 and Jacobs Creek). The environmental parameters strongly correlated to the macroinvertebrate guilds/trophic levels were temperature, conductivity, nitrate, dissolved oxygen, and salinity. These results suggest that changes in benthic macroinvertebrate community structure are driven by environmental variables. Note that for Muddy Creek, water quality is only collected by CZR during benthos collections. For the guilds, scraper was a dominant trophic guild among most of the creek sites (e.g., Drinkwater upstream, Tooley upstream, DCUT 11 upstream, PA2 downstream, and DCUT19 upstream), while filter feeder trophic guild was predominant in Porter Creek downstream. Common scraper or filter -feeder trophic guilds were Littordinops tenuipes (scraper) and Mediomastus sp. (collector filter -feeder, as well as collector -gatherer). Interannual comparisons between pre- and post -Mod Alt L years for guilds indicated no significant spatiotemporal differences among benthic macroinvertebrates for all seven creeks with post -Mod Alt L data. However, Porter Creek, Drinkwater Creek, and Tooley Creek showed differences in pre- and post -Mod Alt L guild structure composition when compared to concomitant composition of years in control creeks. There were less filterer/collector in upstream Porter Creek compared to Little Creek during Porter post -Mod Alt L years (Axis 2. Fiqure III-G21). In downstream Drinkwater Creek, there were more herbivore and detritivore than in PA2 during Drinkwater Creek post -Mod Alt L years (Axis 1; Figure III-G15). There were more herbivore and detritivore in downstream Tooley Creek compared to both Muddy Creek and Long Creek during Tooley post -Mod Alt L years (Axis 1; Figure III-G18). However, these effects were not observed in downstream Porter Creek, upstream Drinkwater Creek, or upstream Tooley Creek, thus it is unclear if changes in trophic level or feeding guild were due to mine -related activities. The nature of a benthic community is variable. Aquatic insect populations may produce single or multiple generations in a year, or the generation time can be longer than a year. III-G-41 In addition, ecosystem function provided by particular taxa may change depending on environmental condition and/or life stage. The life cycle completion time can vary greatly across a species' range and between populations of the same species in upper and lower areas of the same stream. These and other factors may have contributed to the year-to-year variability that has been documented in this study. Such variability cannot easily be attributed to simple changes in hydrographic parameters or habitat structure, and the range of variability documented during the baseline period of this study reinforces the need for careful interpretation of any post -Mod Alt L variation that may occur. III-G-42 Jacks Creek, Upstream (Sweeps) a Jacks Creek, Downstream (Sweeps) b 0 o - o _ onm Group Averages 0 o - o _ Gruup Averages m - I m - I m I _L_ m ( ABC D I E F E ABCD E d 0- 0 N O - ❑ paj m E£ ro m? 3 o ❑ _I 0p N E z b ❑ ❑ I 0 0 N ON E ro% ❑ I 0 N E 3 0 ❑ 0 a E ro 3 0 ❑ 00 N L E rt c 3 0 ❑ N e 0=" ❑ I 0 N' E m ❑ I 0 E m' 3 0 ❑ 0p N E a' ❑ 0 N E E ro ry 3 3 b 0 CI ❑ 0 m N N E 0 ❑ ! NN E 0 ❑ I 0 0p E m 0 `a ❑ 0 N E m i 0 ❑ 0 N E w 0 ❑ I m N E ro 0 I o - 0- 1 0 N O - = m m E E E ro rt E a D>> 1 1 a rn o N E ro a a D= I 1 co- o 0 0 N N E ro a 1 0 0 N E ro a D 1 0 0 N E ro a D>>>=>>>> 1 0 N E T a I W pp N E a 1 co pp [V E ro a 1 po N E E ro m a a 1 Q rn o a N N E a 1 N o N E a 1 -CO 0 N E a 1 r 0 N E ro a D 1 N 0 N E m 4° a D> 1 o 0 N E a I L.11 o h E ro a I Jacks Creek, Upstream (Ponar) C Jacks Creek, Downstream (Ponar) d a a — o_ 40 Group Averages o o — Q _ 0 Group Averages A B I C - m- A B C 1 I E d 0 N O - EEEEEEEEEEEEEEEEEEE m E ❑O I N C) N 1 rt m m i i ❑O ❑ I cr,1 'p N N m u, ❑ ❑O 1 pp' Si N 2 m m m m y 0 1 1 2 m rt m ,n m i 0 ❑ I I O' N N ° m ,n ❑ 0 1 O O.p N N m m � w I ❑ I I fV cc ro s m or m i g ❑ ❑ 1 I pM1p N N N ro £' y b 0 ❑ I C] Q N N ro 2' n v, 0 I O N 2' r"n 0 1 O'-p— N 1 m E ❑ 1 N w t5X m m g ❑ 1 m E 1 E Fu v - N 0 - mnmnommnmonnomponom NI a RI' N E m E a WI E E ro ro m g, ��ffii a Q1 o N' E ro 2' 2 a ml ml rn o N E m °' a NI 0 N E m � a �I 0 N E ro rt m p, a QI 0 N E � yy 2 p_ ml pp 0 2 N E ro a of 0 N E m m � a �I pp N E w yy rr�� a 04 4 N E E yy a Q of NI a o N N E m � a MI 0 N E m m .I 4 N E E m ro � m 4 a of 0 N E ro a o N Figure III-G1 a — d. Dendrogram of clusters based on benthic taxa richness and abundance for all years in Jacks Creek: a) six clusters in upstream sweeps; b) five clusters in downstream sweeps; c) three clusters in upstream ponar grabs; and d) three clusters in downstream ponar grabs. Bold years are post -Mod Alt L and black lines represent significant cluster structure. III-G-43 a 2 E .(7) 0 0 0 - r 0 co 0 CO 0 7r 0 N 0 - A Jacobs Creek, Downstream (Sweeps) Group Averages B C 2021 Downstream 2015 Downstream 2019 Downstream 2016 Downstream 2017 Downstream 2011 Downstream 2013 Downstream 2014 Downstream 2018 Downstream 2012 Downstream 2020 Downstream Figure III-G2. Dendrogram of clusters based on benthic taxa richness and abundance for all years in Jacobs Creek: Three clusters for downstream sweeps. Bold years are post - Mod Alt L and black lines represent significant cluster structure. III-G-44 Drinkwater Creek, Upstream (Sweeps) Group Averages A B C E E E E E E E E E E E a a a _ 2_ a a a a a =I =] =I =I =I =I =I =I =I =I T r 17 0 0 0, O N C N o N O O O o O O O O O pp N N N N N N N N N a 0 0 - 6 0 - Drinkwater Creek, Downstream (Sweeps} Group Averages A E E E E E E E E E E E w 5 5 ; 2`0' 5 d d 5 2 Wz2O2 W2 N O w b b 0 a ❑ ❑ ❑❑I❑ ❑ 0 0 ❑I ❑l 8 I o1 0,vI .1 1 N0,mr N � 0 0 0 0 El OO N O O 0 N N N N N N N N N b Drinkwater Creek, Downstream (Ponar) Group Averages - A IB m E N ❑ V 0 E E E E E E E E E £ E m d v d m d 0 0 0 a 0 0 0 0 0 O. ❑I 0 ❑I ❑I ❑I 01 01 ❑I ❑I ❑1 N COIfJ fO r O C'1 V .I,N N a 0 o a o {o 0 0 0 0 O N N N N N N N N N N N Figure III-G3 a - c. Dendrogram of clusters based on benthic taxa richness and abundance for all years in Drinkwater Creek: a) three clusters for upstream sweeps, b) two clusters for downstream sweeps, and c) five clusters for downstream ponar grabs. Bold years are post -Mod Alt L and black lines represent significant cluster structure. III-G-45 Tooley Creek, Upstream {Sweeps) a Tooley Creek, Downstream (Sweeps} b 0 o - v _ w Group Averages A B CD E 0 o - v _ w Group Averages ABC D E E E ❑ v — O 0 - NI- N - 0- ❑OOO❑O❑❑O❑❑❑❑❑❑ cn EEEEEEEESEEEEEE m 3 3 N 4 NI N 3 rn .,- m o N m 3 n r'n O N m 3 3 Yl oI N r/1 w s rI oo N N W 3 N N N g 2 I N rt 3 ro E m N m 2 oa N N I m m 2 Ir N N rt cu m Ol ❑ a N 3 1 N 0 - m0NoT0m�<0F000 O6pp EE m ro 3 m a E EE w W 3 3 a d E. I I f N m 3 a I EE ro ra m a NppppppppN m a l EE m 3 a I m 2 2 I E w d a I E m a I 11 EEEEE m 2 a m E 2. I m 2 m 3 1 m a Tooley Creek, Upstream {Ponar) c Tooley Creek, Downstream (Ponar) d 0 o - O- Group Averages 0 o - O - Group Averages 1 A 8 m_ A e CD I F m- 1 1 2 — II N o q- N 0 - m1 N CCEE m? m� 7> pN O EE m m m �' 7 7 j o m1 .1 1 rn o 3 3 N E ro ee �g� 7> o NI p N N E of O N EEEEEEEEE m 7? pN N 1 col N ro m 7 41 p N II m m 7>>> �I N m m [czY m n1 N m m 7 n_ p N m � 7ft .1 O p o 7_ N 0 O rn m E m N co $$ Q I rn 3 E ro m g; o I O N EEEEEEECEEEEEE m m 3 ❑o o ?,, m � 3 CO 2 I Ps.O N N o I N w m m �' 3 xx O pW O m � zz;;;;$ �b Q F N N N N O I CO N m m2 O I Q p m � zz O I N N ar CI Q 4'J N N rt m 3$$ O t7 . m � Figure III-G4 a — d. Dendrograms of clusters based on benthic taxa richness and abundance for all years in Tooley Creek: a) five clusters for upstream sweeps; b) five clusters for downstream sweeps; c) six clusters for upstream ponar grabs; and d) two clusters for downstream ponar grabs. Bold years are post -Mod Alt L and black lines represent significant cluster structure. III-G-46 Huddles Cut, Upstream (Sweeps) a Huddles Cut, Downstream (Sweeps) b a o m _ Group Averages A B o o - _ Ea m Group Averages A B C D E 5 ___L m r V N 0 - v' ❑ O N E W t0 l "� is ❑ '''' rp N E na 13 O [D E ttl m 0 O N E y 3 ❑ pWp E N w ! 30 ❑ 0,1 N E N el 2! m 30 ❑ N 2 E v, 3o ❑ ,I N E W t6 m 1120, w is 0 Lol E na w i 0 O N N E m i! v, 3 0 . E W of E 0 O O E Sv H 3 0 O O E N w 3 0 N N E£ b ❑ O O g to (0 w2 b ❑ .- O E t6 v) N 3a ❑ E c0 N ✓+ E i ❑ N 9 9 E 2 m Q N 0 - E E ? N p m n a 7 7 0 O rn o N E to m Sv a 7 7 N o N E N a D o M E E N 2 2 a °, 7 e, C N ro , 7 RI o N E t4 a E ' a 7 7 O WI 0 0 N N E 1 to a 7 m o N E ltl E a 7 ,- R E a 7 r, o N E 2 8 a 7 m 0 N E g >u a 7 0 N E N a r o N E @" m a 7 7 011 E fu 7 P1I O 4 N N E W n 7 fOJ E N m n Huddles Cut, Upstream (Ponar) c Huddles Cut, Downstream (Ponar) d O - o_ m Group Averages OO - Group Averages A BC D o_ m .?, m '-^ m- A B C D E N v o . ❑ N p EEEEEEEEEEEEEEEEEE 3a ❑ m N l O ...mi.,' O ry m N 3 ❑ r ON m C l O 0... O m 0 3 ❑ 6i 63 m h g ❑ 0 O ro N 30 ❑ 0 b N i ❑ CD C'' N l O 6 rt ra N 3 O COO N E 3Q ❑ CO. m W ❑ OD tV — m m W C 30 g ❑ [QV rt G 30 ❑ a CO N N m N , ❑ N 1 ❑ N Cp N m N 1 I E1 N I a o _ v.:N EEEEEEEEEEEEEEEEEE ns a 7 o. N m a 7 7 ., m, N O N N m a 7 w� ppppppN°ppN m a 7 03 ro rt a a 7 V, ro'.'! a 7 7 4I N' a 7 N a 7 o �, O m a 7 OI rn n 7 0 a a 7 o, O a 7 �, p a 7 r, O N m a 7 CO O N m n 7 p m a 7 0,r-, p m a Figure III-G5 a - d. Dendrograms of clusters based on benthic taxa richness and abundance for all years in Huddles Cut: a) five clusters for upstream sweeps; b) two clusters for downstream sweeps; c) four clusters for upstream ponar grabs; and d) four clusters for downstream ponars. Bold years are post -Mod Alt L and black lines represent significant cluster structure. III-G-47 E d0 0 o— Porter Creek, Upstream (Sweeps} Group Averages A B C 2D15_Upstream 2021Upstream 2013_Upstr,eam a E N a 2 — 0 A Porter Creek, Downstream (Sweeps) Group Averages B c E E e N E E N N N N 220 0 0 0 • 0 0 0 0 0 0 0 NI Ni rnl �I N▪ ' I rnl CI 0I I N N N N N N N N N N b cv PA A Porter Creek, Downstream (Ponar) Group Averages W • �G E E E E N EEEEE g g W g N c 33 N y 0 0 0 0 O ❑ ❑ ❑ ❑ ❑ ❑ 0 0 ❑ ❑ 0 rnl ] MI �I rl COI NI I pN N N N N oon N N N N N N N • N Figure III-G6 a — c. Dendrogram of clusters of benthic taxa richness and abundance for all years in Porter Creek: a) three clusters for upstream sweeps; b) four clusters in downstream sweeps; and c) two clusters in downstream ponar grabs. Bold years are post -Mod Alt L and black lines represent significant cluster structure. III-G-48 DCUT11, Downstream (Sweeps) Group Averages A 6 E E E E D 3 C b D ❑ ❑ ❑ ❑ 1 [a in v o 0 o 0 0 N N N N a E E Sv � 0 0 DCUT11, Upstream (Panay) Group Averages B E E E E E N N E 2 a_d d 2 Q d 770 777 I 1 I 11 1 1 1 N Q' to N a col rr o 0 p o 0 0 0 0 N N N N N N N N b Figure III-G7 a - b. Dendrogram of clusters of benthic taxa richness and abundance for all years in DCUT11: a) two clusters for downstream sweeps and b) two clusters in upstream ponar. Bold years are post -Mod Alt L and black lines represent significant cluster structure. III-G-49 Little Creek, Upstream (Sweeps) a Little Creek, Downstream (Sweeps) b 0 o - o _ m Group Averages A BC ❑ 0 o - o _ w Group Averages a 2- A B C 0 a m- E73 r I N d - Q O - v I v o _ N o_ E N t O ❑ ❑ I N N E mr6 2 b I�NeoI N E N O 0 O N E ; S b 0 O N E b 2 0 O N E N C ❑ GE C 2 b ❑ wIu�ImIr N O N N 2 b E ; N g ❑ CI O N E E ;m N g ❑ _ _ N N 1 o _ N o_ rtl I I E E E E N am 2 g' a a a 7 7 7 7 I I 1 I N CI 0 N N E a 7 I N N E a 7 I Q 4] N E b y 7 I r.- nn E b y 7 I E a 7 1 N E m a 7 1 m E mr6 a 7 I m ry Little Creek, Upstream (Ponar) c Little Creek,Downstream (Ponar) d a a - m - Group Averages A BC a a - m - Group Averages A B N E N V n O _ N _ O EEEE g m t ❑ .I N N g m 2 0 0 1 NI N O O N N m g 2 ❑ o N � g 2 0 1 01 N 01 N E m � 2 0 m1 o N E m 2 ❑ ..1 E g � 2 ❑ �I 0 N E g � 2 ❑ ml 0 0 N N SE d g 2 ❑ o N E g m d E 2 2 0 d �I O N d O _ V o _ N o - 7 NI O EEEE m m a 7 NI a lV ro ra m a 7 rl pp N m a 7 NI o N E m � a 7 .0 0 N EEEEEEE m � a 7 NI. o N m � a 7 VI O N g 2' a 7 ml p N 2' m g g a 7 VI O N g m a D rl O N a 7 WI pp N m m O Figure III-G8 a — d. Dendrogram of clusters based on benthic taxa richness and abundance for all years in Little Creek (control): a) four clusters in upstream sweeps; b) four clusters in downstream sweeps; c) two clusters in upstream ponar grabs; and d) three clusters in downstream ponar grabs. Black lines represent significant cluster structure. III-G-50 0 E do 0 PA2 Creek, Upstream (Sweeps) Group Averages A B ro m m ro m E E 6 5 5 r� m m W' �q �Wp' a ava a a a _a a Q a a a =I =1 =I =I =I =I =I ❑I =I =I ▪ N m Q O 0 t17 !� W N 0 0 0 pN N N N N N N N N N N a 0 N 6 0 O— PA2 Creek, Downstream (Sweeps) Group Averages A 8 C D E EEE 0 of 101 FI N N O O O O N N N N 2015 Downstream E 4 E £ £ E 13 E 2 2$ b N} N b 0 0 0 1 ❑1 ❑I 0I ❑I 60I N r> •r co 0 0 O O 0 O N N N N N N b PA2 Creek, Downstream (Ponar) Group Averages A B C o E ro E E E E rt �?, N m � c 5 0 0 0 0 �S �S5 g o ❑1 ❑I 0 1 ❑I ❑I ❑I ❑I ❑I ❑1 ❑1 ❑1 o m r co ,n w v N co N N 0 0 0 00 00 0 0 0 2 0 O N N N N N N N N N N Figure III-G9 a — c. Dendrogram of clusters based on benthic taxa richness and abundance for all years in PA2 (control): a) two clusters in upstream sweeps; b) five clusters in downstream sweeps; and c) four clusters in downstream ponar grabs. Black lines represent significant cluster structure. III-G-51 A Long Creek, Upstream (Sweeps) Group Averages 0 E E E E E E N E E N N N Qi �i Q1 N N Q 0. 0. 0. 0. 0 2 2 0. 71 =I =I =I =I =I 0 N N T 0) N r CO 4 O O o a o 0 o p N N N N N N N N N 2411 Upstream 0. a A B Long Creek, Downstream {Sweeps) Group Averages c o E E E E E N E E E E E N 8 Q1 N N N cif be. 3�3 E gg b O i i i b g i E gg b N fD Qe C] KS V IrJ n o N N N N N N N N N N N N b Figure III-G10 a — b. Dendrogram of clusters based on benthic taxa richness and abundance for all years in Long Creek (control): a) five clusters in upstream sweeps and b) four clusters in downstream sweeps. Black lines represent significant cluster structure. III-G-52 Muddy Creek, Upstream (Sweeps) a Muddy Creek, Downstream (Sweeps) b 0 O _ COm Group Averages AB CDE F 4 v _ Group Averages A BC DE F G H '� 1 o _ N 0— EEEEEEEEEEEEEEEEEEEEEE£ m 12, a��a>v��P��v� r 2 g 0000000000000000CIO 1 1 N m 0 0 00003000,0000000000000000 N N I w of r ,n g 1 1 co CO 0 m N,- N 2 g g 1 1 N 0 N N N w m r y g$ g b 1 1 0 O N ry N g 1 0 1,- O N N g m N g g 1 1 O O 0 N N m g m12,��v� m 2 $$ t 1 1 M V 0 0 N N ra 2 ,e g bO 1 1 10 0 0 N N m 2 gg 1 1 i.- V N N ro m n: a�m�mp y 33 g 00000 1 1 10 m N N m ra g g 1 1 CO 0. N N 2 N gg 33 b O 1 1 N N o _ N o— 7 mo o 01 9- £ E E E£ m m w '�r2�'vmy��uS,��2'm2'��2'2'yy��m2� Q 2_2 Q 2_ 7 7 7 7 7 1 1 1 1 1 vrn NQir o 0 rn 0 a 0 00,0000 N N r N N E E m w 2 Q 7 7 1 1 N N E E m r� 7 7 1 1 W� N 00 N N E£ w ns Q� 7 7 1 1 tiro O 00 N N i E E m ra ro M r�, Q d 7 7 1 1 �n my O 000 N N E E Q d 7 7 1 1 ,i1 0 N N E£ W Q 7 7 1 or�� N O 000 N N E E W ry p_ 2_ 7 7 1 1 roa 00 N N E E m m d 7 7 1 1 mw 0 00 N N E E W mu2y�' Q 2 7 1 1 0 N Muddy Creek, Upstream (Ponar) c Muddy Creek, Downstream (Ponar) d o o - Group Averages o o - ,- 0 Group Averages 0- 1 1_ I 1 A B C D p- I `. .- AB CDE E d o- vd o_ N 0 - N n 7 7 1 N v 4 4 N N 5 i D7 1 1 r N N E 77 1 o N 4 Or N E 5 N nu�2."d 77 D 7 7 1 1 1 m w a rn m o T O N — E N 7 7 1 1 o O N o N N E 7 1 m 0 4 N E i Q"'a 7 7 7 1 1 rn w o 4 O 4 4 4 N N N — 6 E 2.a 7 7 1 1 A N 4 4 N N L 2.; 7 7 1 1 1 W N 4 4 4 N N m E ��an"d a 7 7 1 1 t7 n 4 0 4 4 N N E 7 7 1 n m 4 4 N N 1 E 5 5 77 1 1 m m 0 4 4 N N I 21 v o _ o N 0 - N E 6 O O 0 10 W N rn 0 m N 4 N E E b 0 1 1 W c'l O 4 N E 5 N b t 0 0 0 1 1 1 O rn O 4 4 4 N N N E 0 00 1 V N 4 4 N N N E g 1 1 al D rn Oi E m b O O O 1 l N W 0 4 4 4 4 N N N m E b O 0 1 1 ti o 4 4 4 N N N E 0 1 1 m Ln 4 4 N E 1 E b b O 0 0 1 1 1 N v 4 4 4 4 N N N — g 0 0 1 ,p w O 4 4 N N NE E 0 1 1 r 4 N — 0 W 1 4 N Figure III-G11 a — d. Dendrograms of clusters of benthic taxa richness and abundance for all years in Muddy Creek (control): a) six clusters for upstream sweeps; b) eight clusters for downstream sweeps; c) four clusters for upstream ponar grabs; and d) five clusters for downstream ponar grabs. Black lines represent significant cluster structure. III-G-53 a �L E .N co ifi 0 0 — 0 a) 0 co 0 N Q — DCUT19, Downstream (Sweeps) Group Averages A B 2021 Downstream 2015 Downstream 2017 Downstream 2016 Downstream 2019 Downstream 2013 Downstream 2014 Downstream 1 2018 Downstream 2020_Downstream Figure III-G12. Dendrogram of clusters based on benthic taxa richness and abundance for all years in DCUT19 (control): Two clusters for downstream sweeps. Black lines indicate significant cluster structure. III-G-54 Duck Creek, Upstream (Sweeps) Group Averages A B C E m m E m 2 2 2 E a a a a a a n n a a Q mI 1 (el pI �I ml I 0I 0I NI ,-I N N N N N N N N N N N a, 0 m T � E o � 0 N O Duck Creek, Downstream (Sweeps) Group Averages A B C D E E E E E E E E E E E w w m gg b gg g O o g g g g g b o OI O OI 0I 0I 0 DI 0 01 OI ❑I O rf1 o m tr m N C'1 N 0 0 0 0 0 0 o q p o 0 N N N N N N N N N N b 0 ro 0 Duck Creek, Upstream (Ponar) Group Averages B C 1 E E N m W N c n a n a a a 0_n n n n 71 DI =I =I =I =I DI =I 71 71 =I a, o r- m CO N Ci O I[1 N 0 0 0 0 0 0 o p 0 0 0 N N N N N N N N N N N Figure III-G13 a - c. Dendrogram of clusters based on benthic taxa richness and abundance for all years in Duck Creek (control): a) three clusters for upstream sweeps; b) four clusters for downstream sweeps; and c) three clusters for upstream ponars. Black lines represent significant cluster structure. III-G-55 m O A W d Jacks Creek (Upstream) Group Averages 6 c D E F EEEEEEEEEEE 2itl " ` 21 '0 0 m a a a a a a a a a o I �I �I of TI I �I N�I Lt,I 05 peV 0 0 0 0 o O o o pN o o o p to N N N N N N N N N n EE g a c a az N N a 0.5 — ao -0.5 -1 5 1.5 1.0 y 0.5 • 0.0 -0.5 -1.0 05 N 0.0 • -0.5 0.5 0.0 -0.5 0.5 N 0.0 -1.0 Jacks Creek (Ponar Data) 1 1 1 1 1 I I I I I 1 I I I I I I • I 1 1 I I I I I I I I I I I I I I I I I I • I I � I • I . I g I I I I I • I 1 I 1 • 1 1 1 1 1 1 1 1 1 I • 1 1 1 1 1 I I r 1 • 1 . 1 I I 1 tite I 1 I 1 1 1 1 1 1 1 1 1 1 • 1 1 1 1 1 1 1 1 . • I • 1 1 1 1 • 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 • 1 1 Downstream 1 1 1 1 • 1 1 1 1 1 1 1 1 1 Upstream 1 1 1 1 1 1 1 1998 2000 2002 2004 2006 2008 2010 2012 2014 2016 2018 2020 ME PE MCA •1teCerl t Poe Post Pre Pat Pre Post 1 P re Post Pre Pat Pre Jacks Comparisons (Upstream) Pre POET PIe Poet Pre Pal 8 4 11 -1.5 — Pre Pule Pre Post Pre Pale Jacks Comparisons (Downstream) o 0.0 - 6 att. 5 ! Medley T • 4 -05 - 1 1 1 1 1 1 Pro Poet Pre P50 Pro Poet 0.5 - aa to - .0.5 - 1 T T 11 Q • • A 4 . P re Post Pre Peet Pre Pus, Pro Pal Are Poet Pro Pal 15 os - 00 w -a- Pre Pole Pie Post Pre Past c Pre Pat Pre POP Pre Peet 1.5 - -05— • • T • 10 • Pre Post Pre Pat Are Post Pro Post Pre Pat Pre Pat Jack. to- 0 5 - 4 - 010..00 a , 1±▪ 1 0S Pre Post Pre Posl Pre Figure III-G14 a - c. Dendrograms of clusters of FCA scores (bold year are post -Mod Alt L) and temporal depiction of those scores weighted by relative abundance of each species in Jacks Creek: a) seven clusters in upstream ponar grabs; b) temporal depiction within the six axes of FCA scores of Jacks Creek upstream and downstream ponar grabs; and c) pre- and post -Mod Alt L boxplots of six axes of FCA scores for Jacks Creek years compared to same years in Muddy and Little creeks (controls). III-G-56 8 Jacobs Creek (Downstream) Group Averages E v 2 2012_Downstream m m E E E E 09 1:1�I ❑I ❑I �I CD CI • N O O O O Fr Er (V ON ON N N N a 0.5 0.0 -0.5 -1.0 -1.5 1.5 1.0 y 0.5 ▪ 0.0 -0.5 -1.0 0.5 u) 0.0 -0.5 -1.0 0.5 0.0 N • -0.5 -1.5 0.5 -0.5 -1.0 co < -1 -2 Jacobs Creek (Ponar Data) 1 1 1 1 ! I 1 a I 1 1 1 • ! I • a I 1 . I e I P • 1 I I i I I I 1 I 1 1 1 1 1 I I I 1 • .i 1 I 1 1 1 • 1 • 1 1 1 ! 1 1 1 1 I _— Downstream Upstream' • 1 I I H 1 I 1 1 1 I I • 1 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 PE. DE i PAu OFL POEM I K MORPA PAW i lJH Lag • PM PMt Pr* Pal PM Pset PA.1 Lens, 1 • • Poe Pat Po Pal Pre Post 1 1 • PAR r;r Lane 4. Pre Pore Pre Port Pre Pope Pie Port Pre P09t Pre P. Jacobs Comparisons (Upstream) 05 - 0.0 - Pre Pal PM Pat Pre P051 Pre Pore Pre Post Pre Post o.D - g .0.6 - Jacobs Comparisons (Downstream) Pre POre Pre POW Pre Poet D0 - -05 PAR T I # • L e•e PM P0e1 PM POSE PM Pon Pre Pat Pre Poet PM Pat Pre Pat PM Prat Poe Post 0.0 - -0.5 J.orbs '-r PAZ L an Pre Pore Pre Pore Pie Port L a•• 0.0- I I t I t Pro Post PM Pore PR POSt Figure III-G15 a — c. Dendrograms of clusters of FCA scores (bold years are post -Mod Alt L and temporal depiction of those scores weighted by relative abundance of each species in Jacobs Creek: a) two clusters in downstream ponar grabs; b) temporal depiction within the six axes of FCA scores upstream and downstream ponar grabs; and c pre- and post -Mod Alt L boxplots of six axes of FCA scores for Jacks Creek years compared to same years in Muddy and Little creeks (controls). III-G-57 co 0 Drinkwater Creek (Upstream) Group Averages 0 C E E E E E E E E E E E o_ a a a a a u `8_ o_ o_ n =❑ I 1 1 1 1 1 [ ▪ 1 1 1 Cl u1 fp+ 0N N OD N 0 N • N • N CV N N N N 0 N N a 0.5 -1.0 -1 5 1.5 1.0 co 0.5 0.0 -0.5 -1.0 0.5 0.0 -0.5 -1.0 0.5 0.0 N -0.5 -1,5 0.5 -1.0 Drinkwater Creek (Ponar Data) • 1• = I I 1 1 • • 1 I I I • • I I I i e I 1 • • 1 • 1 1 1 1 8 1 • 1 : 1 • I I 1 1 I • I 1 I • I • I 1 • I I ■ I I I I 1 I I I • I I I 1 1 I • • I I I I o I I — DOWnstream Upstream • • I I 1 I • 1 • 1 1 1 1 1 • I N 0- 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 HE, OE PA CA Go.SC.SH c SH.SC sH E.C,GR.PA PA SH x GR A CO O 0 Drinkwater Creek (Downstream) Group Averages 201 2Downstream B E w 2011 _Downstream C E E E E ozi E E ▪ E eA N (ll [A !11 00 Vi ..I .1 ..I of I f • N • N • N N N Figure III-G16 a — c. Dendrogram of Drinkwater Creek clusters of FCA scores (bold years are post -Mod Alt L), temporal depiction of those scores weighted by relative abundance of each species, and pre- and post -Mod Alt L comparisons to control creeks: a) three clusters in upstream ponar grabs; b) temporal depiction within the six axes of FCA scores upstream and downstream ponar grabs; and c) two clusters in downstream ponar grabs. III-G-58 1_0 — 0.5 — H 0.0 — 7 0.0 O nn Kwater PAZ T T 1 -0.5 — - 10 — Pre Post Pre Post Pre Post 1.0 — 05 — 1 0.0 - 0.5 — O rinkwater I I I 1 I I i c L PA2 Long - 1 0 — Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post 1.0 — 05 — -0.5 — Drinkwater Z I � 1 i PA2 Long _L -1 0 — Pre Post Pre Post Pre Post Drinkwater Comparisons (Upstream) 1.0 — 0.5 — [V y 0.0 — Q Drinkwater PAZ T - 1-0 — Pre Post Pre Post Pre Post Pre Post Pre Post Pre Past 1 0 — 0,5 — t7 00 0.0- Q 19 0,0 - Orinkwater 1 PAZ a 8 - 1 0 — Pre Post Pre Post Pre Post 1,0 — 05 — - 0,5 — Drinkwater _L PA2 Long - 1 0 — Drinkwater Comparisons (Downstream) rJ Pre Post Pre Post Pre Post 1-0 Long 1.0 — 0.5 — 0 0.0 — ▪ 0.0 - 1-0 — Orinkwater T T 1 _L PA2 e I Pre Post Pre Post Pre Past 1.0 — 0.5 — - 0.5 — 1-0 Drinkwater PA2 Long Pre Post Pre Post Pre Post 0,5 — 0.0 - Q IA Id. 0,0 - Lrinkwater � T i PA2 Long T _L - 1 0 — Pre Post Pre Post Pre Post 1.0 — 05 — - 0.5 — - 1 0 — Orinkwatr 1 PA2 Long Pre Post Pre Post Pre Post Figure III-G17. Pre- and post -Mod Alt L boxplots of six axes of FCA scores for Drinkwater Creek years compared to same years in Long creek and PA2 (controls). III-G-59 0 A N 0 0 0 Tooley Creek (Upstream) Group Averages EEEEEEEEEEEEEcts a en 0 N IA N . Cl Q- 0_ n q n n n n N 1 1 1 1 N I 1I0 1 1 I I 0 O C7 1I1 W N f� D O V rp b 0 0 Q b b 0 m q p b O - b b N N N N N N N N N N N N 2011 _Upstream a E 05 00 -0s -1,0 -1 5 15 1A 0,5 0,0 -05 -t0 0.5 N 00 ▪ -0,5 -1,0 05 0,0 -05 •1.0 -1,5 0,5 I 0.0 ▪ A.5 -1.0 CO < -1 Tooley Creek (Ponar Data) - 1 1 1 1 1 1 1 1 1 1 1 1 1 ' • s 1 1 I 1 1 1 1 1 1 1 1 1 -r 1 1 1 1 1 1 1 - 1 1 1 1 • 1 1 1 1 1 1 1 1 1 1 1 1 i. 1 1 • 1 1 1 1 1 1 1 1 1 1 ----- -♦ 1 .- 1 1 1 1 1 1 1 1 1 1 }ri f_` 1 1 1 i--- I 1 1 m--"���-1 1 1 II 1 1 :_ i 1 [ 1 1 1 1 [ 1 1 1 1 1 I • 1 1 1 1 1 1 1 1 1 1 1 S• 1 _L �� 1 Downstream Downstream 1 1 1 1 1 • • 1 1 1 1 1 1 • 1 •= 1 1 1 1 — Upstream 1 1998 2000 2002 2004 2000 206E 2010 2012 2014 2016 2018 2020 HE, DE P!1 C0. FG sx s0pa,PA Pk9H SC cF1 ax 8 d w N O _ Tooley Creek (Downstream) Group Averages D E F EEEEEEEEEEEE N C 2 C C N N C N N C 1 y N N N W III 1 1 1 1 1 1 I I 1 CO 0O .1.W O W LL'1 1- Q! O C7 N m O N O N O o CD a o O o L2 a p 4 a O 4 a N N N N N N N N N N N N c Figure III-G18 a — c. Dendrogram of Tooley Creek clusters of FCA scores (bold years are post -Mod Alt L) and temporal depiction of those scores weighted by relative abundance of each species: a) two clusters in upstream ponar grabs; b) temporal depiction within the six axes of FCA scores upstream and downstream ponar grabs weighted by relative abundance of each species; and c) six clusters in downstream ponar grabs. III-G-60 1,0 — 0.5 — .X 00- -0,5 — Tooley Mundy T I " L. Long T PA2 III -1,0 — Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Tooley Comparisons (Upstream) 1.0 — 0.5 — N 00- -0.5 — Tooley I 1 Muddy Long PA2 1 -1.0 — Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post Tooley Comparisons (Downstream) 1.o - 1.0 — 05 — .% 00- Tooley T Muddy Long PA2 -0.5 — Pre Post Pre Post Pre Past Pre Post 1,0 — 0.5 — -0 0,0 — -0,5 — Tooley I I1 1 O Muddy Long 1 PA2 Pre Post Pre Post Pre Post Pre Post Pre Past Pre Post Pre Post Pre Post 1.0 — 0.5 — w 0.0 — -0.5 — Tooley Muddy ▪ T 11 g Long PA2 I I 8 Pre Post Pre Post Pre Post Pre Post 1.0 — 0.5 o) X 00 — (0 05 Tooley O T Muddy Long • I 1 PA2 -1.0 — Pre Post Pre Post Pre Post Pre Post 1.0 — 05 — N 00 — Q O) -0 5 — Tooley Muddy Longo PA2 - 1.0 — Pre Post Pre Post Pre Post Pre Post 1.0 — 05 — .% 00 — a r0 00 — x - 0.5 — Tooley 11 T Muddy Long l: PA2 Pre Post Pre Post Pre Post Pre Post 1.0 — 0.5 — a -0 5 — Tooley 1 T Muddy Long PA2 Pre Post Pre Post Pre Post Pre Post Figure III-G19. Pre- and post -Mod Alt L boxplots of six axes of FCA scores for Tooley Creek years compared to same years in Muddy and Long creeks and PA2 (controls). III-G-61 1n a Huddles Cut (Upstream) Group Averages B C D E F EEEEEEEEEEEEEEE N r6 N ro N tri 8 W W to @ 0 rt 8 10 r6 m � a� m � � m m � E 0� �' m m � a a a a 4 a aa s a a a.a a Q a a a o pQ_I OV CD �1 0 aal �1 w1 NI �1 r0nl m1 N- p�nnommnmnnommmDmonn I r1 c;1 0 N N N N N N0 0. N o N N N N N N N N N N a 05 0.0 -1.0 -1 5 1.0 0.5 0.0 -0.5 0.5 0.0 -1.0 -1.5 0.5 -1.0 -2 Huddles Cut (Ponar Data) a minim I I s . • I I I 1 I 1 I I I 1 • • 1 mini • I I I I I I I 1 in I" I. I I I I 1 1 1 • 1� i • Y 1 1 in I 1 1 1 3 i � 1 1 1 1 r Y 1 1 • 1 1 1 1 1 --"1".--,F 1 1 1 � ---- I 1 1 ter-- 1 1 --- 1 ° • -' 1 1 1 1 1 1 1 1 1 1 1 1 1 • 1 1 •• f 1 , � 1 1 1 I 1 1 1 1 1 •f • I 1 1 1• 1 1 1 ' 1 o 8 1 ie •, 1 1 r I : 1 1 � 1 - Downstream 1 1 1 1 1 • 1 � 1 -___ I Upstream i���;- 1 1 1 1 1 1 1 1 1 � �-�' 1 1 1 1555 2000 2001 2002 2003 2006 2005 2006 2007 2005 2009 2010 2011 2012 2012 2016 2015 2016 2017 2015 2015 2020 2021 HE. OE P0. CA GRSCSH vC 9n.SC 5C.4 ,PA PASH SC Tlcrs i sc co 0 N A Huddles Cut (Downstream) Group Averages B c 1 E E E E E E E E E E E E E 0 0 2 0 m f ? P 0 7 7 '' '' . C11 y N N N '4 Pl cc. Vf In 0 EA e) 0 LA CO 0) 00 1tgg i;i1gg ❑ O ❑ ❑ ❑ ❑ O ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ I I I I I 1 Ni I I I I 4! a0 .- CD W 0 0 eD �D O f� C7 W 0 O O O (V N 0 0) O 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 N !V N (V N N N N N N N N N N N N.- N c ri EEEE ro d Figure III-G20 a — c. Dendrograms of clusters of FCA scores (bold years are post -Mod Alt L) and temporal depiction of those scores weighted by relative abundance of each species in Huddles Cut: a) six clusters in upstream ponar grabs; b) temporal depiction within the six axes of FCA scores upstream and downstream ponar grabs; and c) three clusters in downstream ponar grabs. III -G-62 1,0 - 0.5 - 00- Q -0 5 - Huddles IJ Muddy I -1,0 - Pre Post Pre Post 1,5 - 1,0 - • 0.5 - 9.0 - Huddles I-- H Muddy Pre Post Pre Post Huddles Comparisons (Upstream) 1.0 - Muddy 1.0 - 0.5 - X 0.0 -0.5 - -1.0 - Huddles H h I Pre Post Pre Post 1.5 - 1.0 - 0.5 - 0.0 - N .55 0.0 Huddles T I 1 1 Muddy -1.0 - Pre I I Post Pre Post 0.5 M .k 00 tD• - 0 5 - Huddles T T I I I I i Muddy -1.0 - Pre Post Pre Post Huddles 1.5 - 1,0 - 0,5 - 0.0 - a Muddy - 1. 0 - Pre Huddles Comparisons (Downstream) 1.0 - 0.5 - -0.5 - Huddles H I Muddy Pre Post Pre Post Huddles 1.5 - 1.0 - 0.5 - 0.0 - Muddy Post Pre Post 1.0 - 05 - co 00 - CD to - 0.5 - Huddles Muddy Pre Post Pre Post Huddles 1.5 - 1.0 - 0.5 - 0,0 - Muddy a + 8 Post Pre Past Pre Figure III-G21. Pre- and post -Mod Alt L boxplots of the six axes of FCA scores for Huddles Cut years compared to same years in Muddy Creek (control). Pre Post Pre Post III-G-63 6 A 0 6 6 Porter Creek (Upstream) Group Averages B C mE m E E Ea E E n a a a a a a a a a 71 =1 =1 77 1 1 ro N co COcv CV a r- u7 CD o a o 05 0 0 b b b CV CV CV CV CV CV CV CV CV a 0.5 r 50 YI •1.0 -1 5 1.5 1.0 H 0.5 0.0 -0.5 -1.0 0.5 0 0.0 -0.5 -1.0 0.5 -1.5 o-s -1.0 CO 0 N .x < -1 2 Porter Creek (Ponar Data) al • Y I I I I I I I I I I I • t rc 1 1 I 1 1 1 I 1 r 1 1 • • s • • • Er I I I 1 r 1 I I 1 1 I la --r - ors s . • • • 1 1 1 1 1 1 1 1 1 1 I n • St I I I I 1 1 I I 1 1 I _— Downstream Upstream ;- of w ec 8 10 00 10 O 0 0 6 A Porter Creek (Downstream) Group Averages B C ❑ EEEE mE co N 10 2 2 2 2 W N N N N C C C C C !z$✓ C C Czz C O • O O 0 0 b b O a ❑I ❑I ❑I ci ❑I ❑I ❑ ❑I ❑1 ❑1 ❑I a N V9 Ci7 CO *DI CV a a (V b b a 6 a Q 0 0 b b O CV CV CV CV CV N N CV CV CV CV C 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 Figure III-G22 a — c. Six axes of FCA scores, pre- and post -Mod Alt L comparisons to control creeks, and dendrogram of clusters of FCA scores in Porter Creek: a) four clusters in upstream ponar grabs (bold years are post -Mod Alt L); b) temporal depiction within the six axes of FCA scores upstream and downstream ponar grabs weighted by relative abundance of each species; c) four clusters in downstream ponar grabs (bold years are post -Mod Alt L). III -G-64 Pre Post Pre Post Pre Post 1.5 - 1,0 - 05 - - 0.5 - - 1,0 - Pre Post Pre Post Pre Post 1,0 - 0.5 - 0,0 - Porter T li 1 a 1 Duck 1 i Little - 05 - Pre Post Pre Post Pre Post 1,5 - 1,0 - 05 - V 0.0 - - 05 - Porter I. ▪ T Duck 8 T � I 8 Little a T -1 0 - Pre Post Pre Post Pre Post Porter Comparisons (Upstream) o- Pre Post Pre Post Pre Post 1.5 - 1.0 - 0.5 - 0.0 - Porter Duck Li tte 1.0 - 0.5 - 0.Q - -0.5 - Porter 1- • Duck Little -1_0 - Pre Post Pre Post Pre Post 1.5 - 1.0 - 0_5 - rD uer -0.5 - ,.0 - Pre Post Pre Post Pre Post 0.0 - - 0.5 - - 1.0 - Porter Duck Little Pre Post Pre Post Pre Post Porter Comparisons (Downstream) 1.0 - 0.5 - 0.0 - -05 - Porter • Duck T I I Little -1.0 - Pre Post Pre Post Pre Past 1.5 - 1.0 - 0.5 - 0.0 - -0.5 - Porter Duck Little 991 -1.0 - Pre Post Pre Post Pre Post 1.0 - 0.5 - • 0.0 - 05 Porter T II11 � L i 1 Duck Little • T 8 8 -1_0 - Pre Post Pre Post Pre Post 1.5 - 1.0 - 0_5 - 0.0 - - 0_5 - Porter Duck T Little - 1.0 - 7 I 1 I Pre Post Pre Post Pre Post Figure III-G23. Pre- and post -Mod Alt L boxplots of the six axes of FCA scores for Porter Creek years compared to same years in Duck Creek (control) and Little Creek (control). III-G-65 0 A v 0 DCUT11 (Upstream) Group Averages C D E E m m E m 2 m 2 2 2 2 a o_ - - - a a a a 0 01 =1 =1 ❑1 =1 01 =1 =I CD CO M1 COLALo 0 N 0 0 0 0 0 0 0 0 CH N N N N N N N N a co 0.5 0.0 -1.0 -1 5 1.5 1.0 0.5 0.0 -0.5 -1.0 0.5 0.0 -0.5 -1.0 0.5 0.0 -6.5 -1.0 -1.5 0 -1 -2 DCUT11 (Ponar Data) r —��� I I I I I 1 1 1 I I ■ I I I I • I I 1 I I I I • I • I I I I I. • I I I I I I. 1 I I --� — 1 I 1 1 1 1 I I I. Downstream t. Upstream *.1{ 1 I 1 1 1 1 I i I I• HE, OE PA CA CR,SC.SH FC sH ii $C,CR,PA PASH sc aR aic 0 0 A N 0 DCUT11 (Downstream) Group Ave ages B C 2 y '2 2 . . N Vi eA o g o g g o f III❑I ❑I ❑II(Vrz.Lo vr O O b N • N • N N CV N N c 2013 2014 2015 2016 2017 2018 2019 2020 2021 Figure III-G24 a — c. Dendrograms of clusters of FOA scores (bold year is post -Mod Alt L) and temporal depiction of those scores weighted by relative abundance of each species in DCUT11: a) five clusters in upstream ponar grabs; b) temporal depiction within the six axes of FCA scores upstream and downstream ponar grabs; and c) three clusters in downstream ponar grabs. III-G-66 1.0 - 0.5 _ s 0.0 - DCUT11 T 1 1 DCUT19 T 1 Duck Pre Post Pre Post Pre Post 1.0 - 0.5 - 0.0 - DCUT11 V 1 T DCUT19 Duck B 1 1.0 - I I I I I I Pre Post Pre Post Pre Past 1.0 - 0.5 - 52 0.0 - ▪ 0.0 DCUT11 T 4 8 1 B DCUT19 11 11 Duck T -0.5 - t0 - Pre Post Pre Post Pre Post 1.0 - 0.5 - 0.5 - DCUT11 I i DCUT19 Duck T B 0 1 1 1 1 I Pre Post Pre Post Pre Post DCUT11 Comparisons (Upstream) 1.0 - DCUT19 T 0.5 - I 11 os- i P o_o - u7 .52 0.0 - DCUT11 1 1 Duck 1.0 - 0) o_o - -0.5 - 8 -0.5 - Pre Post Pre Post Pre Post 1.0 - 0.5 - OCUT11 DCUT19 Duck 1.0 - 1 1 1 I Pre Post Pre Post Pre Post DCUT11 Comparisons (Downstream) 1.0 - 0.5 - N 0.0 - • 0.0 -0_s - Dcur11 11 1 8 8 DCUT19 T 1 Duck 0 1.0 - Pre Post Pre Post Pre Post 1.0 - 0.5 - DCUT11 T T e 1 DCUT19 Duck DCUT11 DCUT19 1 1 Duck Pre Post Pre Post Pre Post t 1 1 Pre Post Pre Post Pre Post Pre Post Pre Post Pre Post 1.0 - 0.5 - 0.0 0.5 - 0.5 - 1 1 1 Pre Post Pre Post Pre Post DCUT11 DCUT19 Duck 0 1 1 Pre Post Pre Post Pre Post Figure III-G25. Pre- and post -Mod Alt L boxplots of the six axes of FCA scores for DCUT11 years compared to same years in DCUT19 (control) and Duck Creek (control). III-G-67 B Little Creek (Upstream) Group Averages £ E E EEEEEEEE a a a a a a a a a a a ❑I =I =I ❑I ❑I =I =I 71 7= 1 I C7 N a1 a 117 m O 1- a" a 0 N 0 o a N o a o 0 0 0 N N N N N N N N N N N a to 0.5 -1.0 -1 5 1.5 1.0 0.5 0.0 -0.5 -1.0 0.5 0.0 -0.5 -1.0 0.5 0.0 N -0.5 [ri -1.0 -1.5 0.5 0.0 -0 5 -1.0 0 -1 -2 n Little Creek (Ponar Data) [ I • I I ! 1 I I I I ! I I I I I I • I I I I 1 1 1 • 1 1 • • • I I I I ! 1 I I I I I • I I I • I • I I - ---- 1 1 1 1 1 �-•� 1 - . 1 1 • 1 - 1 1 --- ! — Downstream v Upstream 1 1 • • 1 - 1 I • 1 1 1 • I • 1 I PC SCARP., O b Little Creek (Downstream) Group Averages B C D E E m E E EEEEEEE 0 m m c N N Vi N N N N N N N N C C C C C C C C C C C 0 0 0 0 0 0 0 0 0 0 0 ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ �! �I NI al noI �I I I �I of N b b NI b b O O b b N N N N N N N N N N N 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 Figure III-G26 a - c. Dendrogram of clusters of FCA scores and temporal depiction of those scores weighted by relative abundance of each species in Little Creek: a) three clusters in upstream ponar grab; b) temporal depiction within the six axes of the FCA scores for upstream and downstream ponar grabs; and c) five clusters in downstream ponar grab. III-G-68 10 0 N a A PA2 (Upstream) Group Averages c o E EEE E EEEEEEE N 10 N N N N N N N N N o_ a a a a a a a n n a 7 > > > 7 7 = �I 01 �I �I NI �I al �I rl �I b O O b b b b b b b N N N C11 C1 N N N N N N a 0.5 A— 0.0 -0.5 -1.0 -1 5 1.5 1.0 N 0.5 ▪ 0.0 -05 -1.0 0.5 N 0.0 • -0.5 -1.0 0.5 0.0 N -0.5 -1.0 -1.5 0.5 100 ▪ -0.5 co fp -1.0 0 -1 PA2 Creek (Ponar Data) X! 1 t = n • . I 1 1 I I I I I 1 I I • • . E . ec I 1 1 I I I I 1 1 I I • E • f , • 1V 1 1 1 1 I 1 r 1 1 I 1 e • • 1 1 1 1 1 1 1 1 1 1 1 it 1 I 1 I I I [ 1 1 I 1 — Downstream • Upstream • sc 1 1 1 1 ] I I 1 1 1 . e s 0' .scs+ •c utM 0 c 1 3D 0 V 0 A PA2 (Downstream) Group Averages B r E E E E E E E E E E E c to N N [A N N N N N N N i C C C C C i C C C 0 0 0 0 0 g g O ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ ❑ of al Nl ,,,,IM1I �I WI rl �I �I N • N b b b O b b b b b b b N N N N N N N N N N N 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 Figure III-G27 a - c. Six axes of FCA scores and dendrogram of clusters of FCA scores in PA2: a) five clusters in upstream ponar grabs; b) temporal depiction of FCA scores weighted by relative abundance of each species; and c) two clusters in downstream ponar grabs. III-G-69 0.5 0.0 -6.5 -1.0 • 1 5 1.5 1.0 N 1n 0.5 • ▪ 0.0 .0.5 -1.0 0.5 0.0 N -as •1.5 0.5 0.0 ▪ -0.5 -1.0 N -2 Long Creek (Ponar Data) 1 1 I wit t 1 I - 1 1 1 I 1 1 1 1 1 • I I • 1 1 • 1 I 1 I I I I • 1 I I I 1 I I I - ;y I I I • I - • I •._ 1 • 1 I I I 1 1 1 1 1 It 1 1 1 1 1 l _— Downstream Upstream • 1 I J 1 1 1 1 1 1 aL 8 0 A Long Creek (Downstream) Group Averages 6 b m m m m m N N N !q 0 0 N VI N C C C C C C O O O O 01 ❑1 ❑1 ❑1 01 01 fl m r a In 09 0 0 o a o 0 CV CV CV CV CV CV 0 N n 0 E m N 01 01 0 0 o a N IN CV 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 Figure III-G28 a - b. Dendrograms of clusters of FCA scores and temporal depiction of those scores weighted by relative abundance of each species in Long Creek: a) temporal depiction within the six axes of FCA scores upstream and downstream ponar grabs and b) four clusters in downstream ponar grabs. III-G-70 8 o O O ABC Muddy Creek (Upstream) Group Averages E E E E E E E E E E E E E E E E E E E E E E E ra m ra m m m m rt m m m m m m a� '�' � m '�' m m �' m m� � m� m m� m a� � dJ a N N 0 (0 N «I N N [A N N N (l] N N N N N N N N tl] N an_n 000_0_00_ ana anaa1?so_ a a an 7 777=777D=7=====7==7==7 1i11111i111 111 111 111 11 O Cr C] CO r tq I) r C) N 1 - 41 CD CO O O Cr N 9- CA co Q! 1 - O O O O NO 6] O O O O O Q 4 O O O O O O O 0 Q�1 O CV CV N CV N r (V N N CV N CV CV CV CV CV N CV CV CV N r- CV 0.5 0.0 -0.s -1.0 -15 1.5 1.0 0.5 0.0 -0.5 -1.0 0.5 M Vj 0.0 ▪ -0.5 -10 0.5 0.0 -0.5 -10 -1.5 0.5 11, 0o ▪ -0.5 -1.0 No Muddy Creek (Ponar Data) • . . • M • • 1 1 1 1 1 1 1 1 1 1 1 1 N 1 1 1 1.1 1 1 1 1 1 1 • .• • ■ • • 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1•1 1 1 1 1 1 1 1 • . • �� 4 • J :ice__. -• . • • • 1 1 1 1 1 I 1 I 1 1 1 1 1 I I 1 1 1 1 1 I 1 1 I • 1• • an . : • 1• • ac . 1 1 1 1 1 I 1_1 1 1 1 1 1 I 1 1 1 1 1 1 1 1 1 I M n f f,✓i'y •� am- �!— . , 1 1 1 1 1 1 1 1 [ 1 1 1 [ 1 1 1 1 1 1 1 1 1 1 • Downstream w Upstream 1 1 III III II 1 1 1 1 r 1 1 1 1 1 I 1 De OkM V 0 N O B Muddy Creek (Downstream) Group Averages E F G H C E E E E E E E E E E E E E E E E E E E E E E ra m m ra m m ra m m ra m m � m m ra m m ra m m ra mme a�emmermm2r 2m20m22m20m rD (0 «1 N N «) [0 V) ffl N N N to (J] EA rA (1) EA rA N N N] (1) CCE C CC C CC CCE C CC CC C CCE C C o g g o g g o g g o g o o g o o g a o g o o g ❑ ❑❑❑❑❑❑❑❑❑❑❑❑❑❑❑❑❑❑❑❑❑❑ 11 1 I 1 1 I 1 1 1 IJI I I I III I I I co W O ro O 0) C7 O Co r- C7 9- N V ti CO 0 CO N 47 CV O O Q1 N 0 0 O 0 0 0 O rn O o 0 o O o rn o o O o o g o o O o 0 0 0 4 0 • N N N N N N N N N CV N N CV CV N C1 CV CV N CV CV 1998 2000 2002 2004 2006 2008 2010 2012 2014 2016 2018 2020 Figure III-G29 a - c. Dendrogram of clusters of FCA scores and temporal depiction of those scores weighted by relative abundance of each species in Muddy Creek: a) four clusters in upstream ponar grabs; b) temporal depiction within the six axes of the FCA scores for upstream and downstream ponar grabs; and c) eight clusters in downstream ponar grabs. III-G-71 ro 0 DCUT19 (Upstream) Group Averages A B C a o- o_ - n n o- o_ n 71 71 71 71 71 71 71 77 1 ▪ 1 N in C7 (V r• 0)07 Ca 4 4 o d o o d CV CV N N N N N CV N 0.5 -1.0 -1 5 1.5 1.0 H 0.5 0.0 -0.5 -1.0 0.5 N 0.0 " -0.5 -1.0 0.5 0.0 N -0.5 0.5 -1.0 CD 0 DCUT19 (Ponar Data) 1 • 1 1 1 • '� 1 1 1 1 1 1 • I 1 1 1 1 I 1 1 1 I 1 1 1 1 I 1 1 1 1 1 • 1 i• 1 I 1 1 1 1 1 ifs. 1 I 1 1 Downstream Upstream _3._________i�____� t- F 1 1 1 1 HE, DE P.. c FC SH $C A,PA ac 00 00 0 0 0 A 0 0 DCUT19 (Downstream) Group Averages B c y N N N N N N N N C C C C C C C f C o0 0 I❑I ❑IIf ❑1 CO rC1co CDO O▪ O O • CV CV N • N N CV N 2013 2014 2015 2016 2017 2018 2019 2020 2021 Figure III-G30 a - c. Dendrogram of clusters of FCA scores and temporal depiction of those scores weighted by relative abundance of each species in DCUT19: a) three clusters in upstream ponar grabs; b) temporal depiction within the six axes of the FCA scores for upstream and downstream ponar grabs; and c) three clusters in downstream ponar grabs. III-G-72 1� 0 8 6 o m O — 0 0 A Duck Creek (Upstream) Group Averages E E E E E E E 8 a a a - a a `a a a n n a 7 7 7 7 7 7 7 7 7 7 I h] co of NI to co pl )I I 1 0 0 0 0 o 0 0 0 o 0 o N N N N N N N N N N N 0.5 6.0 -0.5 -1.0 -1 5 1.5 1.0 6.5 6.0 -0.5 -1.0 0.5 -10 -1.5 -1.0 2 Duck Creek (Ponar Data) • - I I I I • I 1 I I I 1 I I I I I I I I I I I I I 1 I I I 1 I I I I I I I I I I I I I _ • 1 I I 1 1 I I I 1 I I I 1 I — .Downstream • Upstream 1 1 1 1 1 1 1 1 1 I 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 N C GC Pk CA ac Figure III-G31 a —b. Dendrogram of clusters of FCA scores and temporal depiction of those scores weighted by relative abundance of each species in Duck Creek: a) two clusters in upstream ponar grabs and b) temporal depiction within the six axes of the FCA scores for upstream and downstream ponar grabs. There were no defined clusters for downstream. III-G-73 a) a) a)> vi o I— (n d (6 O ru ai x LC) > 92 —) a) O .5 U c c1 • c c-0 a) C a) p a)L O cn _1L a) 4 c 1 U C w •0 L) O O a U U C)cQ " 0 O O E N N U a) ca J c Y E-' > p A) c o U U 5 a;as a) o L L L = _a U a) OU ca Etca c0� (-) .2 o 0 '- U C E o Z o m 8 6 > Q'� C o : a) 0_ CS)o O L — 4 .- D U a) D a) Ct • • Uo NQO (/)� caUN C Z .0 o0 > >, > CIS 0 - - 6O)- - c H a) cn D ,� c — 0 - NO a 1N O N c O N E D C a) 6 N c) a) cN x D M (> C.) c)C . > a) us N O N co 0 -0 -0 (6 C -0 cac E N c 1 o a) 4' U U C L a) Q U C a) _ o cc a) 6L � cs p p ifs' B 2001 and 2007-2021 in a) 0) a) c .c co j O O CO CO 010101 O M O O N n N CO V N N N N N 01 CO CO , N N COCOCO010100 N N N N N 01 N N N V LL' 0 h E n '2 h m 0 h 2. 42 cc c h E hc _ u m > -E. (N h m m y .2- GIm 2 N�° °E ° h a'y o 0._ a« h ,c c h N i..N `.'mc, a hEL3 h 4,cNmmh, '4" -a -2 a ° x «,c, m E ... maa° 0m m N 0 m o N N N -o osN �° >>>0• m 22 m •• .:---- .; Nm m nNa n 5 m y y h m m a,Nc° 2 c c c o h h h mN h w o o, ch t caca" m hm .m moa m° o m ,m m m m m,c a m m E a-m m h h h m o m m a a am2 2 2 m `o t ,,m 0mmm0o° m 0 y y m ai ai NBU.mNc t0 m UU,m mm ° ,LE w a @N a N `� � � A-0 L Qm 2 0 EE:E 173 a L% 2 % t t 2 a B L o o m .° B N "• B E m a m m ... 0 0 m m m m Q odg o ° ° ° : a .E-8 L- o 0 aE m E y m am 19 m 0 E E E .c - .Em-- o- -2 m° 8_ 0 o t D Gm o Q 0 CO CO W Z Q. Q. Q. Q. CO 0 Q. 0 E 0 0 0 o .Q Q .o,, 2 0 0 0 E UUU_ ,, W= Q, 0 0 0Q 0 CO CO 0 o E 0 W2 mW O; r 0 a,c0 a a u o E a a U � O m @ 0m a U m 0a a CO m o a H.' To a m 0 I— a` -.2 m c 0 o ' O N a,� a ° - 412 c> -o c aa o .m-mo 0.c a Nm m m .o m u m m o `c c o o 2 2 o c c o m c N ««aamm(0(0 N0a -mNNB (o ` "m. N , s am m m L'4'4 72 0 m ,` °` aaau�a, 2 m " -a Q. W o a 0 0 m 0 oo W oW W W W:a, g O 0 a m -o COc _ N c a m 0 _. °m N E a, h m.o .-2.=E h h h .2.- m o- a 0 N° N m m m" -o m m U U U a,= N??N0B m 0 72°°m m 72 E E E m o m o = i ' om- -0 0) am QJ= 0- 0 E m fly a= a, a m m 2 F m a 0 S CO j 0N O co. N m V N O m m O N V N N O m V O r N co co co co co (O m N N N N N N N N N . LL. O . h Qcm o mQ "-E u Lg so0000'o ... m Q mQ m o I- a a h> 0 h N o o m E' 2 m hcw h h N m>o0^� mN mm m «° h N a i0 c h° NNoN ca h a c vo E cOcOE G Ea sx " c m Nmco N wNN N 0. ° N h 0 so n > h m8 s N h Q o m tm:s me •QN x> o• cs mm'N"a 0 o0 «� m x x x x x x co h E ym E cay ,o m m m E 0$2 0>, hc o h h a a c N h h c o, N h h -0 c m am ENh 0 m a LO N h Nm m m m mN N •o00mm m m h m o z c m m -m m 0) N m N-m mmmQ°a o mon Nuhhhh o EE o'am m m m m 0 No2o m. oo t..0m o (0 E G,G,mS.c0.c0UCBcN o ")m 2.Nm:2s-m02 'o 0 0 0 0 m000000h0 0 oom 2� o o o o`0 0 O m GG - mGm.E w'0'-0h O .Q 0 wE0 0 76 L 0 -p 'o 0- `"E 76 0,2 6 0 yom xO0 0 0 0 0 0(` 8 2 "0 O m0 0 T ,DU .I.Q cn DoQ mZUU W00ZWWWtiQQu ',W omOQ010fl-OI-Emro m .-Qc0a0a0a0.C01=1=1=1=1=1= UD;D;I-1-Qm -o ZZ oWW W mn D; D; D; °EZ m W a, W ° @ o n im 0 0 w CO F F L Q O E m 0 0 x = z z o_ o_ 0) > n0 m 0a 0, 0 w 1 _I U m m tN ot mmh mo aNN . s'mN mm o N o 0 -o m 2, ..208; mm Ao = E E m . -(0) (0) aQ Q Q m 0 0aF , 0 0 0 -o < 00 j V M. LL� LL� (O ( O O O O) . CO CO CO m V O O O O) O (O O O m m COLL� 0N N M M i N N N V N N LL� CO C-- CO CO O O LL� V V N O r O O .-.- CO N N CO N N m m N h o 2 to 2 u c , Q `,'2' m h • > u Not h .�o h '- O 2 2 2 Q mo co co >> Nya sNo m o� E Q y Na 0 m c m m h = 0/c E wac o 2 m.S N " d ro o 2N N y 2: o a-o m oo Q z ah ioao00oaEEEEEE>>Qth.00 2 2 h cNN2 o E ,.1) 2- ° ws0L a m a ow0° o •a, c o o" o N ,0a,0 hhhhhhhwEEE a,, a,, a,, ym ,,, my -2 a ux'>>>>E°aaaaaaa a a a o 0 0 o m m>>>> am m 2 m h m 2 c c c c c 2 Zh m 2 0 m o m.N mmm! m •_ ° ' a 2 kJkJ2 2 2 2 2 o GG p N N« "O 2 -p U= 2 2 2 2 2 U .p «««««T a m m- N 70 •Nc 72 0a 0 a 4, °'1 "m°°72 E a w U «2 N N N N N N .6 t t-2'«U U U U -2-2 Z Z Z Z Z Z a `.1NNLof°E20 a0 4.4.4.4.4.a,°m0 0Umm0.OU`N0 Q °N2 2 2 2 -8 -8 Q Q 0 0 0 0 0 0 2 2 O'O'OQ Q Q N NNN iiiiii2. N Q D -° 00o -"6'8 "Q. ' p Q N N O 0 m m m 2 8 RRRRRR 0 0 0'' -8 -8 ro ro W W W=3 1= 1= 1= 1= 1= -`=om W'2 U 2 2 2 -.<- m 0 0 IL Et. E) E) 2. Q -E) -E) -E) 0 U U -a. -a. -a. -a. -a. -a. O W W W W UUUUUUUY Z Z L LU 0- x S S z z a a 0) (n n U 0 0 m t o x Z 1a` a Q 0 v o aaaahwa o c.'c= EN ° . E 0- " 0:o mE E - m 2 c o_ o_ c h 2000 =oQ ..°Euu0c uu, i s0000 c. GG>G > > >>o •;ct..>>>>m= > °o,.,aE LLLL= >>>> O a a o o N • 2 N c0000000mamc o 0 Q Q NNN 'O O 0 0 0 0 0 O%O2 2 o 2 2 G R RRO a. a. ce 10 ti ti 'F.-E. 1-- U U U U U U U U a. Z=0 D j V V O O O. 0 0 0 O O . O m O oO O O I� O O O N M N N CO N m. N O O V oO oO N V V V V N N N 01 N N w 0 m m h m t• m h m � s oro2: c us E � h m • �' a:Q c O -c-c, 4.2 °°Nn °' ° m n L°o22 vt w jmN0Na ao • m m m2E ,n0mnm - a>a a - nos N0h m N0hh'mmu u m 2 2 "a m -o m m m mm4m4m4s sobN ->> >m m m ooN2 ' u �«aaam -- m a, a, , , , -@N NN�aN22 Ey .h 0 0 cm �o tcco m t m -0m s Q.. s 7 o 0 0=-8 a, aa, aa, aac c 0 o N" 'mm 2 E--. E-, ' of-,Eo00a Q m .2 u u WLD m 2 ._o 0 0 .o p m , m -m o -m De >o E E 2 2 ym m� ,o h.2'8 '8 s a-8 o m� m0 m Q=OEo 0 m ` Ea O E 0=_= 2 Ym -E' ° (o Q B Q Q J J Z Z Z Q. Q. Q. W I- - f. m 0 1' J Z Z . W W m .2_ E' Q On m m :2 w a a zt z z z a z a m m a co xc`Q ° ma U 0 W CO Q Ti 12 0 w h m c y h ym .- him au E m o , a, h a E hw m 'o c h a, m w w m ,.2.. c m > ,0aO m h c0_ «h m• ,o3 3,n,n .2 n w Nm ocaE h -0 aQv 2- N c E°o, h o u ° E m -h 0�' o w ha m o oao m' .. .. a sQ m m m • Nc0 m h 0 " os .'N u m • 0c. m QmN2 to ° mm..00-Ev=ti c ,n 2 ,,, -ai -1 24 mma °m m m m o 00 0 om mmm .o :0E Emmao_ u N aa-a, .-m 7 2 oo o -m 0 0 o Ns 2 o c= 2 m 0 2. -2 m� _0 2 0 -o a a a a 0 ,o 0 0° 2-o m m m aam m 0. :am22a m s' 2 a y 2ap a a J2m Ec m m W W co co 2 .Q 0 Z Q J J co V B Q E a, Q "O ZQ E m.< "O W•Eo m m §, J •„U U i i Z co f. ', O a, a, a, a, oU 0 o.=E Q = Eoz a co oUU o x CO 2 == a F0 m 0 s 0 a III-G-74 a) U 0 a) H A u) O V O O N N— N N i i i i (0 ri i In V O i i M M lf') N M O O N N— V lf') N— lf') N— O N i 0 i i i i N LO M N— N— O O N N 0 i i i i M N Taxa Balanidae sp. Balanus sp. Balanus amphitrite amphitrite Balanus improvisus Balanus subalbidus O f6 O a -t 4 Q Q N o o Q O U E N 2 O ( 0 n O U o o a 6 C o 'O C N O o o N .�.. A U ti 'm 0 m a L �. U m E N O N O (p o. O C t o> 0 m m ii e a N n` N N 2 (6 c 0 N O N < Q w (6 (6 'E U O NO 2 Q U U) o o o o N (6 E N o o 'rn (6 O N d (6 N c ti m .ti o m a E m a .c N ii c n aO w m o m o N .N N N N -0 N ti c o - m a 'o m o 0 0 0 co Y o 7 'c O O N Q m o a c a U o w 'O _ O N O C s. U m N O 'T z o N-2 Q. L 2 U= o Q A W 5 >. 2 c 0 CO O a _ 2 > o m m m •a O o '� ti y a o_ m .E ti 0(0 v! Q o U N U ti o ,m m m -0 .G 01 N •E c c (6 Q N Z cr CO 2 m 'O O E N co U ° °� a� E N o co -o = H Q w N E O U N Z m w 'm N E O U (6 Z c) c m° N E O U (6 Z N m O w D O Q o 0 o N Q o O L rn i coa (7 o m Q o W m a N N O O w w �° o aci o -aa Nm ti wE O ° n O o o w m ti �' m m o 0 0 'c o o o a E a m( y c � 'O (6 o E m o N N D N (6 ' ., C Z .5 U 0 U E lL Q. L C 2> Q J a m o CO -o N a o m 7 N A L s U N m a� (q N a a a Q c U C C o °� m Ty) -a a a O c c 'n O O N O �.. �.. o -O J J >' 0 2 z N coti n U y o m m cti U v m .c c t o -o c m w •� w U (6 �- O. .'- o c N 7 ti c ,.. 0 O �. N N 0 J L Q 0 T W -O f6 E .. N J .N 7 N LLJ 0 s Ozu) w aa) aa) z z d N N m -a com := o L C Q o Ocp_ N a oz HH FL' L a) (11 E O L U_ +J C a) a) (Q a) a) N_ (73 (11 () 0 L O aH aH a) LA_ 0 U z a) 0) N N a) (Q N a) (/) III-G-75 Table III-G2. Dominant benthic macroinvertebrate species for sweep samples collected in monitored creeks during May of Mod Alt L sample years 1998-2005 and 2007-2021. Pre -Mod Alt L: Broomfield Swamp Creek (2019-2021), Jacks Creek (1998-2005; 2011-2014), Jacobs Creek (2011-2013), Drinkwater Creek (2011-2012), Tooley Creek (1998-2001; 2010-2011), Huddles Cut (1998-2001; 2007-2009), Porter Creek (2011-2015), and DCUT11 (2013-2017). Post -Mod Alt L: Jacks Creek (2015-2021), Jacobs Creek (2014-2021), Drinkwater Creek (2013-2021), Tooley Creek (2012-2021), Huddles Cut (2010-2021), Porter Creek (2016-2021), DCUT11 (2018-2021). Control Creeks: SCUT1 (2019-2021), PA2, Long, Little, and Duck creeks (2011-2021), Muddy Creek (1998-2005 and 2007-2021), and DCUT19 (2013-2021). A - indicates the creek was not monitored that year. Benthic collection occurred in June for 2010. The count for each species was stopped at 100 individuals per sweep. WDominant W W 0 a Lu -I Et W H L. N 2 d 0 0 m 1998 1999 2000 2001 2002 2003 2004 2005 Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Littoridinops sp. Polypedilum sp. Corixidae sp. Candonidae sp. 40 31 29 23 13.7 10.6 9.9 7.8 Gammarus tigrinus Mytilopsis leucophaeta Naididae w/o hair sp. Cyprideis Iittoralis 300 169 130 122 37.1 20.9 16.1 15.1 Corixidae sp. Naididae sp. w/o hair Tanytarsus sp. Mytilopsis leucophaeta 108 82 12 11 41.0 31.1 4.5 4.2 Ma WDominant W 2 U cc W13 IX J I- W Z 2 0 0 0 Cl m 0 Ce 2 ZIQ 0 0 I- dDominant I- M 0 1998 1999 2000 2001 2002 2003 2004 2005 Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - l - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2015 2016 2017 2018 2019 2020 2021 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored 1998 - - not monitored 1999 - - not monitored 2000 - - not monitored 2001 - - Corixidae sp. Cyprideis littoral's Naididae sp. w/o hair Dero sp. 2002 109 83 73 63 14.6 11.1 9.8 8.5 Gammarus tigrinus Cyprideis littoral's Naididae w/o hair sp. Americamysis almyra 2003 300 204 100 82 36.5 24-8 12.2 10.0 Gammarus tigrinus Tanytarsus sp. Dicrotendipes nervosus Naididae w/o hair sp. 2004 89 70 40 33 23.4 18.4 10.5 8.7 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored not monitored not monitored - - not monitored - - not monitored not monitored 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2015 2016 2017 2018 2019 2020 2021 Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - - Naididae sp. w/o hair Corixidae sp. Goeldichironomus holoprasinus Tanypus neopunctipennis 127 45 40 30 31.7 11.2 10.0 7.5 Cyprideis littoral's Gammarus tigrinus Naididae w/o hair sp. Physidae sp. 300 300 207 52 31-8 31.8 21.9 5.5 Naididae w/o hair sp. Corixidae sp. Chironomus decorus Tanytarsus sp. 207 164 61 43 35.8 28.3 10.5 7.4 SCUT1 (CONTROL) DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Gammarus tigrinus Cassidinidea lunifrons Kiefferulus sp. Candonidae sp. 133 116 99 59 18.0 15.7 13.4 8.0 Gammarus tigrinus Americamysis almyra Cyprideis littoral's Amphicteis floridus 300 227 203 108 30.1 22-8 20.4 10.8 Gammarus tigrinus Amphicteis floridus Dicrotendipes nervosus Littoridinops sp. 300 41 28 23 67.3 9-2 6.3 5.2 III-G-76 Table III-G2 (continued). 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus 300 11.3 Gammarus tigrinus 300 38.4 Amphicteis floridus 300 12.3 Amphicteis floridus 300 10.6 Littoridinops sp. 300 30.6 Chironomus decorus 300 25.2 Cyprideis littoralis 300 13.5 Amphicteis floridus 290 15.1 Littoridinops sp. 300 11.3 Littoridinops sp. 163 20.9 Cyprideis littoralis 300 12.3 Cyprideis littoralis 300 10.6 Goeldichironomus devineyae 115 11.7 Goeldichironomus devineyae 300 25.2 Littoridinops sp. 300 13.5 Gammarus tigrinus 288 15.0 Enallagma sp. 261 9.8 Cassidinidea lunifrons 123 15.7 Gammarus tigrinus 300 12.3 Gammarus tigrinus 300 10.6 Enchytraeidae sp. 111 11.3 Littoridinops sp. 257 21.6 Tanytarsus limnecticus 288 13.0 Tanytarsus sp. 1 276 14.4 Amphicteis floridus 201 7.6 Cyprideis littoralis 97 12.4 Dicrotendipes nervosus 300 12.3 Littoridinops sp. 300 10.6 Palaemonetes pugio 88 9.0 Cyrenoida floridana 81 6.8 Amphicteis floridus 253 11.4 Littoridinops sp. 253 13.2 W 2 2007 2008 2009 2010 2011 2012 2013 2014 WDominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total U IX not monitored - - not monitored - - not monitored - - not monitored - - Chironomus decorus 300 8.5 Littoridinops tenuipes 300 34.7 Nematoda sp. 300 16.9 Littoridinops tenuipes 300 27.5 Cr) I- - - - - - - - - - - - - Apocorophium sp. 300 8.5 Goeldichironomus devineyae 237 27.4 Littoridinops sp. 284 16.0 Chironomus decorus 141 12.9 V.. - - - - - - - - - - - - Littoridinops sp. 300 8.5 Apocorophium sp. 126 14.6 Chironomus decorus 247 13.9 Corixidae sp. 131 12.0 Q - - - - - - - - - - - - Apocorophium louisianum 266 7.5 Chironomus decorus 65 7.5 Goeldichironomus devineyae 216 12.2 Gammarus tigrinus 96 8.8 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Littoridinops sp. 300 19.1 Corixidae sp. 300 18.7 Littoridinops sp. 300 22.9 Littoridinops sp. 300 14.0 Chironomus decorus 300 12.1 Apocorophium sp. 300 18.2 Littoridinops sp. 257 28.5 Apocorophium sp. 293 18.6 Littoridinops sp. 300 18.7 Corixidae sp. 188 14.3 Gammarus tigrinus 300 14.0 Corixidae sp. 220 8.9 Gammarus tigrinus 300 18.2 Apocorophium sp. 251 27.9 Gammarus tigrinus 286 18.2 Tanytarsus sp. 206 12.9 Gammarus tigrinus 185 14.1 Tanytarsus sp. 272 12.7 Littoridinops sp. 216 8.7 Littoridinops tenuipes 268 16.3 Gammarus tigrinus 123 13.7 Amphicteis floridus 107 6.8 Apocorophium sp. 162 10.1 Apocorophium sp. 149 11.4 Chironomus decorus 266 12.4 Amphicteis floridus 208 8.4 Cyprideis littoralis 214 13.0 Chironomus decorus 47 5.2 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus 295 11.9 Gammarus tigrinus 300 30.2 Littoridinops sp. 128 21.7 Gammarus tigrinus 300 14.6 Littoridinops sp. 246 29.7 Americamysis almyra 148 19.0 Littoridinops sp. 239 23.6 Apocorophium sp. 300 21.8 Cricotopus sp. 252 10.2 Apocorophium lacustre 245 24.6 Cricotopus sp. 102 17.3 Littoridinops sp. 300 14.6 Americamysis almyra 100 12.1 Littoridinops sp. 124 15.9 Cyprideis littoralis 132 13.0 Littoridinops sp. 300 21.8 Cassidinidea lunifrons 235 9.5 Cassidinidea lunifrons 170 17.1 Apocorophium lacustre 77 13.1 Dicrotendipes nervosus 230 11.2 Palaemonetes pugio 62 7.5 Apocorophium lacustre 109 14.0 Americamysis almyra 129 12.7 Gammarus tigrinus 264 19.2 Y 2 Littoridinops sp. 224 9.0 Littoridinops sp. 153 15.4 Gammarus tigrinus 73 12.4 Goeldichironomus devineyae 200 9.7 Laeoneries culveri 56 6.8 Amphicteis floridus 89 11.4 Tanytarsus sp. 1 103 10.2 Americamysis almyra 248 18.0 W Q 2007 2008 2009 2010 2011 2012 2013 2014 Ce Ce Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total U H not monitored - - not monitored - - not monitored - - not monitored - - Apocorophium sp. 256 12.7 Hargeria rapax 159 19.7 Nematoda sp. 230 26.1 Littoridinops tenuipes 300 18.8 YCI) - - - - - - - - - - - - Palaemonetes pugio 248 12.4 Apocorophium sp. 146 18.1 Apocorophium sp. 115 13.1 Hargeria rapax 283 17.7 V - - - - - - - - - - - - Hargeria rapax 204 10.2 Littoridinops tenuipes 115 14.2 Hargeria rapax 113 12.8 Apocorophium sp. 187 11.7 Q 0 - - - - - - - - - - - - Gammarus tigrinus 172 8.6 Americamysis almyra 97 12.0 Littoridinops sp. 99 11.3 Gammarus tigrinus 178 11.1 0 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus 300 19.2 Apocorophium sp. 300 19.3 Littoridinops sp. 300 39.2 Gammarus tigrinus 300 26.4 Littoridinops sp. 300 13.7 Apocorophium sp. 300 22.5 Littoridinops sp. 204 67.1 Littoridinops sp. 300 19.2 Gammarus tigrinus 300 19.3 Gammarus tigrinus 224 29.2 Littoridinops sp. 208 18.3 Apocorophium sp. 295 13.5 Littoridinops tenuipes 300 22.5 Gammarus tigrinus 36 11.8 Apocorophium sp. 272 17.4 Littoridinops sp. 300 19.3 Apocorophium sp. 124 16.2 Americamysis almyra 180 15.8 Corixidae sp. 247 11.3 Gammarus tigrinus 255 19.1 Palaemonetes pugio 16 5.3 Americamysis almyra 135 8.6 Apocorophium lacustre 269 17.3 Apocorophium lacustre 38 5.0 Gammarus mucronatus 127 11.2 Gammarus tigrinus 183 8.4 Hargeria rapax 211 15.8 Goeldichironomus devineyae 14 4.6 JACOBS CREEK UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Littoridinops sp. 300 18.0 Littoridinops tenuipes 300 31.9 Goeldichironomus devineyae 300 18.5 Littoridinops tenuipes 300 24.0 - - - - - - - - - - - - Apedilum sp. 222 13.3 Goeldichironomus devineyae 228 24.2 Littoridinops sp. 280 17.3 Chironomus decorus 212 17.0 - - - - - - - - - - - - Apocorophium sp. 218 13.1 Apedilum sp. 190 20.2 Chironomus decorus 234 14.4 Goeldichironomus devineyae 156 12.5 - - - - - - - - - - - - Goeldichironomus devineyae 142 8.5 Chironomus decorus 91 9.7 Apedilum sp. 192 11.9 Apocorophium sp. 122 9.8 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Chironomus sp. 300 18.4 Apocorophium sp. 300 19.0 Littoridinops sp. 300 21.6 Apocorophium sp. 300 23.7 Littoridinops sp. 300 25.2 Littoridinops tenuipes 193 26.9 Littoridinops sp. 300 37.9 Littoridinops sp. 300 18.4 Littoridinops sp. 300 19.0 Apocorophium sp. 256 18.4 Littoridinops sp. 300 23.7 Goeldichironomus devineyae 134 11.3 Apocorophium sp. 132 18.4 Goeldichironomus devineyae 165 20.8 Tanytarsus sp. 236 14.5 Goeldichironomus devineyae 138 8.7 Corixidae sp. 252 18.2 Goeldichironomus devineyae 146 11.5 Chironomus decorus 132 11.1 Corixidae sp. 100 13.9 Apocorophium sp. 79 10.0 Apocorophium sp. 222 13.6 Apocorophium lacustre 136 8.6 Trichocorixa sexcinta 136 9.8 Apocorophium louisianum 142 11.2 Tanytarsus sp. 130 10.9 Gammarus tigrinus 88 12.3 Palaemonetes pugio 59 7.4 JACOBS CREEK DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Apocorophium sp. 285 18.9 Apocorophium sp. 220 21.5 Littoridinops sp. 203 16.4 Apocorophium sp. 274 12.8 - - - - - - - - - - - - Littoridinops sp. 201 13.3 Hargeria rapax 174 17.0 Apocorophium sp. 176 14.2 Chironomus decorus 272 12.7 - - - - - - - - - - - - Hargeria rapax 198 13.1 Americamysis almyra 138 13.5 Chironomus decorus 174 14.1 Littoridinops tenuipes 232 10.8 - - - - - - - - - - - - Chironomus decorus 184 12.2 Apocorophium louisianum 134 13.1 Hargeria rapax 151 12.2 Hargeria rapax 224 10.4 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Apocorophium sp. 300 20.0 Apocorophium sp. 300 18.8 Gammarus tigrinus 300 21.3 Apocorophium sp. 300 13.6 Littoridinops sp. 300 21.1 Hargeria rapax 193 21.3 Littoridinops sp. 175 19.7 Littoridinops sp. 300 20.0 Littoridinops sp. 284 17.8 Apocorophium sp. 274 19.4 Gammarus tigrinus 300 13.6 Apocorophium sp. 233 16.4 Gammarus mucronatus 150 16.6 Hargeria rapax 135 15.2 Gammarus tigrinus 243 16.2 Gammarus tigrinus 168 10.5 Littoridinops sp. 241 17.1 Littoridinops sp. 284 12.9 Goeldichironomus devineyae 204 14.4 Littoridinops tenuipes 150 16.6 Gammarus tigrinus 121 13.6 Rhithropanopeus harrisii 108 7.2 Corixidae sp. 125 7.8 Corixidae sp. 126 8.9 Chironomus decorus 252 11.5 Gammarus tigrinus 139 9.8 Gammarus tigrinus 139 15.4 Goeldichironomus devineyae 94 10.6 III-G-77 Table III-G2 (continued). 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total CtQ not monitored - - not monitored - - not monitored - - not monitored - - Apedilum sp. 300 15.6 Apedilum sp. 300 20.0 Littondinops sp. 300 22.9 Littondinops tenuipes 300 30.7 zW ct 0 1-Apocorophium Littondinops sp. sp. 300 262 15.6 13.6 Littondinops tenuipes Goeldichironomus devineyae 300 239 20.0 15.9 Goeldichironomus devineyae Paranais litoralis 240 106 18.3 8.1 Goeldichironomus devineyae Chironomus decorus 204 153 20.9 15.7 0 (1) Gammarus tigrinus 170 8.8 Apocorophium louisianum 205 13.7 Apedilum sp. 102 7.8 Apocorophium sp. 88 9.0 Cs/D 2015 2016 2017 2018 2019 2020 2021 dDominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Littondinops sp. 300 16.9 Corixidae sp. 300 14.7 Littondinops sp. 300 17.8 Littondinops sp. 300 23.2 Littondinops sp. 300 15.0 Littondinops tenuipes 300 23.6 Littondinops sp. 294 26.2 Apocorophium sp. 267 15.1 Littondinops sp. 274 13.4 Corixidae sp. 300 17.8 Apocorophium sp. 216 16.7 Apocorophium sp. 224 11.2 Apocorophium sp. 259 20.4 Goeldichironomus devineyae 252 22.5 Gammarus tignnus 258 14.6 Apocorophium sp. 252 12.4 Apocorophium sp. 230 13.7 Apocorophium louisianum 172 13.3 Goeldichironomus devineyae 204 10.2 Corixidae sp. 234 18.4 Apocorophium sp. 140 12.5 Goeldichironomus devineyae 215 12.1 Gammarus tignnus 236 11.6 Gammarus tignnus 226 13.4 Goeldichironomus devineyae 172 13.3 Chironomus decorus 184 9.2 Gammarus tignnus 142 11.2 Chironomus decorus 140 12.5 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 J 2 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Ctw not monitored - - not monitored - - not monitored - - not monitored - - Littondinops sp. 300 19.8 Littondinops tenuipes 300 22.5 Littondinops sp. 300 23.8 Littondinops tenuipes 300 26.7 I- Ct Apocorophium sp. 242 16.0 Apocorophium sp. 292 21.9 Goeldichironomus devineyae 295 23.4 Goeldichironomus devineyae 236 21.0 Z I- 0 (1) Goeldichironomus devineyae 206 13.6 Apedilum sp. 202 15.1 Apocorophium sp. 185 14.7 Apocorophium sp. 226 20.1 O Z ci 0 Apedilum sp. 132 8.7 Goeldichironomus devineyae 196 14.7 Amphicteis floridus 132 10.5 Chironomus decorus 119 10.6 2015 2016 2017 2018 2019 2020 2021 d0 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Apocorophium sp. 300 20.7 Littondinops sp. 300 17.0 Littondinops sp. 300 22.6 Apocorophium sp. 300 13.7 Apocorophium sp. 300 15.0 Littondinops tenuipes 300 22.7 Littondinops sp. 300 26.5 Goeldichironomus devineyae 300 20.7 Apocorophium sp. 266 15.0 Corixidae sp. 251 18.9 Chironomus decorus 300 13.7 Goeldichironomus devineyae 300 15.0 Corixidae sp. 241 18.3 Goeldichironomus devineyae 266 23.5 Littondinops sp. 300 20.7 Corixidae sp. 234 13.2 Apocorophium sp. 217 16.4 Gammarus tignnus 296 13.6 Littondinops sp. 300 15.0 Gammarus tignnus 201 15.2 Gammarus tignnus 86 7.6 Gammarus tignnus 132 9.1 Goeldichironomus devineyae 228 12.9 Gammarus tignnus 155 11.7 Apocorophium louisianum 282 12.9 Apocorophium louisianum 284 14.2 Apocorophium sp. 200 15.2 Apocorophium sp. 83 7.3 I DRINKWATER CREEK UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Apedilum sp. 300 14.8 Goeldichironomus devineyae 300 29.2 Apocorophium sp. 282 16.9 Littondinops tenuipes 300 24.8 Littondinops sp. 300 14.8 Littondinops tenuipes 300 29.2 Goeldichironomus devineyae 269 16.1 Chironomus decorus 244 20.1 Apocorophium sp. 278 13.7 Apocorophium sp. 142 13.8 Littondinops sp. 258 15.4 Goeldichironomus devineyae 223 18.4 Gammarus tignnus 199 9.8 Apocorophium louisianum 56 5.5 Apocorophium louisianum 230 13.8 Apocorophium sp. 131 10.8 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tignnus 300 22.8 Apocorophium sp. 300 18.1 Gammarus tignnus 300 20.2 Apocorophium sp. 300 16.9 Gammarus tignnus 207 34.7 Apocorophium sp. 255 29.0 Gammarus tignnus 260 20.8 Littondinops sp. 300 22.8 Gammarus tignnus 300 18.1 Apocorophium sp. 295 19.9 Apocorophium louisianum 284 16.0 Littondinops sp. 62 10.4 Littondinops tenuipes 225 25.5 Apocorophium sp. 258 20.6 Apocorophium sp. 262 19.9 Littondinops sp. 266 16.0 Littondinops sp. 226 15.2 Gammarus tignnus 254 14.3 Cassidinidea lunifrons 54 9.1 Corixidae sp. 110 12.5 Littondinops sp. 220 17.6 Amphicteis flondus 96 7.3 Amphicteis flondus 136 8.2 Apocorophium louisianum 183 12.3 Chironomus decorus 228 12.8 Goeldichironomus devineyae 41 6.9 Gammarus tignnus 76 8.6 Chironomus decorus 142 11.4 DRINKWATER CREEK DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Hargeria rapax 300 22.5 Hargeria rapax 180 18.3 Hargeria rapax 216 17.3 Littondinops tenuipes 245 19.1 Apocorophium sp. 150 11.3 Amencamysis almyra 172 17.5 Apocorophium sp. 190 15.2 Apocorophium sp. 193 15.0 Littondinops sp. 146 11.0 Littondinops tenuipes 132 13.5 Littondinops sp. 149 12.0 Gammarus mucronatus 164 12.8 Gammarus tignnus 137 10.3 Chironomus decorus 111 11.3 Nematoda sp. 120 9.6 Hargeria rapax 150 11.7 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Littondinops sp. 300 18.1 Littondinops sp. 300 21.3 Littondinops sp. 300 22.4 Gammarus tignnus 249 16.5 Littondinops sp. 300 33.9 Littondinops tenuipes 230 23.0 Hargeria rapax 180 18.6 Apocorophium sp. 244 14.8 Gammarus tignnus 295 21.0 Gammarus tignnus 276 20.6 Hargeria rapax 206 13.6 Apocorophium sp. 216 24.4 Hargeria rapax 205 20.5 Littondinops sp. 167 17.2 Gammarus tignnus 218 13.2 Apocorophium sp. 228 16.2 Apocorophium sp. 215 16.1 Littondinops sp. 204 13.5 Gammarus tignnus 147 16.6 Corixidae sp. 147 14.7 Goeldichironomus devineyae 155 16.0 Hargeria rapax 207 12.5 Hargeria rapax 142 10.1 Apocorophium lacustre 174 13.0 Apocorophium sp. 193 12.8 Goeldichironomus devineyae 63 7.1 Goeldichironomus devineyae 103 10.3 Gammarus tignnus 127 13.1 III-G-78 Table III-G2 (continued). J O C' I- Z 0 Q `-' W Yce W I- LLI f/) C' 11 U D W J ~ � J 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Littoridinops sp. Tanytarsus sp. Gammarus tigrinus Amphicteis floridus 300 166 127 122 26.1 14.5 11.1 10.6 Enchytraeidae sp. Tanytarsus sp. Dasyhelea sp. Goeldichironomus devineyae 248 185 160 121 20.3 15.2 13.1 9.9 Gammarus tigrinus Littoridinops sp. Tanytarsus sp. Cyprideis littoralis 300 300 216 201 15.0 15.0 10.8 10.0 Gammarus tigrinus Littoridinops tenuipes Chironomus decorus Physidae sp. (physa/physella) 300 285 170 124 25.1 23.8 14.2 10.4 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus Gyprideis littoralis Gyp Naididae sp. w/o hair Littoridinops sp. 300 250 160 59 31.2 26.0 16.6 6.1 Gammarus tigrinus Cyprideis littoralis Tanytarsus sp. Amphicteis floridus 300 274 62 59 29.5 26.9 6.1 5.8 Gammarus tigrinus Cyprideis littoralis Chironomus decorus Littoridinops sp. 300 291 163 120 23.5 22.8 12.7 9.4 Gammarus tigrinus Cyprideis littoralis Mytilopsis leucophaeata Naididae sp. w/o hair 300 241 100 56 29.1 23.4 9.7 5.4 Gammarus tigrinus Corixidae sp. Gyprideis littoralis Mytilopsis leucophaeata 300 234 154 104 24.9 19.4 12.8 8.6 Cyprideis littoralis Gammarus tigrinus Americamysis almyra Littondinops tenuipes 300 300 73 62 34.1 34.1 8.3 7.0 Chironomus decorus Tanytarsus sp. Gammarus tigrinus Amphicteis floridus 131 58 46 43 32.4 14.4 11 A 10.6 Illkom J O C' H Z Q 0 W Y I- WCnPalaemonetes � U O W p J ~ H J 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Apocorophium sp. Littoridinops sp. pugio Apocorophium louisianum 220 152 116 115 16.1 11.2 8.5 8.5 Palaemonetes pugio Apocorophium sp. Littoridinops tenuipes Hargeria rapax 184 168 122 70 21.8 19.9 14.5 8.3 Nematoda sp. Apedilum sp. Apocorophium sp. Hargeria rapax 209 208 130 113 18.1 18.1 11.3 9.8 Littoridinops tenuipes Apocorophium sp. Chironomus decorus Hargeria rapax 300 179 118 67 30.0 17.8 11.7 6.7 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Apocorophium sp. Littoridinops sp. Gammarus tigrinus Rhithropanopeus hamsii 300 300 223 141 24.1 24.1 17.9 11.3 Littoridinops sp. Apocorophium sp. Corixidae sp. Gammarus tigrinus 300 261 187 162 22.7 19.7 14.1 12.2 Littoridinops sp. Cyprideis littoralis Gammarus tigrinus Apocorophium sp. 300 232 196 189 20.0 15.4 13.0 12.6 Littoridinops sp. Gammarus tigrinus Apocorophium sp. Palaemonetes pugio 192 155 77 47 34.0 27.5 13.7 8.3 Littoridinops sp. Gammarus tigrinus Goeldichironomus devineyae Palaemonetes pugio 251 90 52 50 41.1 14.7 8.5 8.2 Littoridinops tenuipes Apocorophium sp. Gammarus tigrinus Tanytarsus sp. 300 288 244 131 21.0 20.1 17.1 9.2 Palaemonetes pugio Littoridinops sp. Gammarus tigrinus Naididae sp. w/o hair 15 13 6 5 25.4 22.0 10.2 8.5 -IDominant O C' I- Z O Q U W Y Ce w f/) Ce Cl- U O Z O J 1998 1999 2000 2001 2002 2003 2004 2005 Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Littoridinops sp. Chironomus decorus Apocorophium sp. Apedilum sp. 300 242 178 156 18.9 15.2 11.2 9.8 Littoridinops tenuipes Apocorophium louisianum Apocorophium sp. Chironomus decorus 220 202 74 54 29.7 27.3 1.0 7.3 Goeldichironomus devineyae Littoridinops sp. Chironomus decorus Amphicteis floridus 252 212 204 68 25.9 21.8 20.9 7.0 Chironomus decorus Gammarus mucronatus Apocorophium sp. Amphicteis floridus 300 232 213 153 18.6 14.4 13.2 9.5 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total / Dominant Species Total / Dominant Species Total / Dominant Species Total / Dominant Species Total / Dominant Species Total / Dominant Species Total Apocorophium sp. Chironomus sp. Littoridinops sp. Tanytarsus sp. 300 300 300 300 12.9 12.9 12.9 12.9 Littoridinops sp. Gammarus tigrinus Apocorophium sp. Amphicteis floridus 300 268 210 128 19.5 17.4 13.6 8.3 Littoridinops sp. Gammarus tigrinus Apocorophium sp. Amphicteis floridus 300 300 202 132 20.8 20.8 14.0 9.1 Littoridinops sp. Gammarus tigrinus Apocorophium sp. Chironomus decorus 300 234 206 200 21.1 16.4 14.5 14.0 Corixidae sp. Littoridinops sp. Chironomus decorus Enallagma sp. 300 242 234 226 10.2 8.3 8.0 7.7 Gammarus tigrinus Littoridinops tenuipes Apocorophium sp. Uhlorchestia uhleri 76 53 32 32 30.4 21.2 12.8 12.8 Chironomus decorus Littoridinops sp. Apocorophium sp. Amphicteis floridus 300 300 158 108 23.2 23.2 12.2 8.4 LONG CREEK (CONTROL) DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total / Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Apocorophium sp. Gammarus mucronatus Gammarus tigrinus Apocorophium lacustre 226 190 117 45 25.3 21.3 13.1 5.0 Americamysis almyra Apocorophium sp. Apocorophium louisianum Nematoda sp. 214 195 120 120 19.2 17.5 10.8 10.8 Nematoda sp. Hargeria rapax Balanus improvisus Gammarus mucronatus 173 163 138 109 22.3 21.0 17.8 14.0 Gammarus mucronatus Apocorophium sp. Rhithropanopeus hamsii Hargeria rapax 241 215 186 162 17.8 15.9 13.7 12.0 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus mucronatus Apocorophium sp. Gammarus tigrinus Littoridinops sp. 236 212 172 148 17.8 16.0 13.0 11.1 Littoridinops sp. Americamysis almyra Apocorophium sp. Gammarus tigrinus 298 202 166 120 25.5 17.3 14.2 10.3 Apocorophium sp. Gammarus tigrinus Littoridinops sp. Gammarus mucronatus 300 300 300 276 19.2 19.2 19.2 17.6 Gammarus mucronatus Gammarus tigrinus Hargeria rapax Littoridinops sp. 257 245 126 124 26.8 25.5 13.1 12.9 Americamysis almyra Littoridinops sp. Apocorophium sp. Corixidae sp. 300 221 112 77 29.3 21.6 10.9 7.5 Gammarus tigrinus Gammarus mucronatus Balanus improvisus Apocorophium sp. 181 126 61 45 34.2 23.8 11.5 8.5 Hargeria rapax Gammarus tigrinus Littoridinops sp. Americamysis almyra 116 105 98 92 17.3 15.6 14.6 13.7 III-G-79 Table III-G2 (continued). TOOLEY CREEK UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Littoridinops sp. 300 16.6 Gammarus tigrinus 300 26.1 Littoridinops sp. 298 13.8 Littoridinops sp. 300 13.2 not monitored - - not monitored - - not monitored - - not monitored - - Gammarus tigrinus 226 12.5 Cassidinidea lunifrons 253 22.0 Chironomus decorus 282 13.1 Goeldichironomus devineyae 300 13.2 - - - - - - - - - - - - Amphicteis fioridus 214 11.8 Littoridinops sp. 195 17.0 Amphicteis fioridus 267 12.4 Tanytarsus sp. 1 300 13.2 - - - - - - - - - - - - Dicrotendipes sp. 181 10.0 Cyprideis littoralis 104 9.1 Gammarus tigrinus 224 10.4 Cyprideis littoralis 224 9.9 - - - - - - - - - - - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - Littoridinops sp. 240 26.1 Littoridinops sp. 300 18.9 Littoridinops tenuipes 300 26.3 Littoridinops sp. 268 16.4 Chironomus decorus 300 15.6 - - - - - - - - - Apocorophium sp. 189 20.6 Apocorophium sp. 202 12.7 Apocorophium sp. 197 17.3 Apocorophium louisianum 224 13.7 Littoridinops tenuipes 297 15.4 - - - - - - - - - Goeldichironomus devineyae 142 15.5 Chironomus decorus 183 11.5 Nematoda sp. 138 12.1 Apocorophium sp. 220 13.5 Apocorophium sp. 262 13.6 - - - - - - - - - Chironomus decorus 85 9.3 Apedilum sp. 164 10.3 Apocorophium louisianum 131 11.5 Goeldichironomus devineyae 204 12.5 Gammarus mucronatus 257 13.3 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Tanytarsus sp. 300 12.6 Apocorophium sp. 300 15.5 Apocorophium sp. 300 11.7 Littoridinops sp. 300 26.2 Littoridinops sp. 300 25.1 Littoridinops tenuipes 300 28.3 Tanytarsus sp. 300 15.3 Hargena rapax 282 11.8 Littoridinops sp. 300 15.5 Gammarus tigrinus 300 11.7 Chironomus decorus 197 17.2 Apocorophium sp. 140 11.7 Apocorophium sp. 267 25.2 Littoridinops sp. 300 15.3 Littoridinops sp. 236 9.9 Gammarus tigrinus 233 12.0 Littoridinops sp. 300 11.7 Apocorophium sp. 191 16.7 Apocorophium louisianum 113 9.4 Gammarus tigrinus 100 9.4 Hargena rapax 288 14.7 Chironomus sp. 220 9.2 Apocorophium louisianum 220 11.4 Cyprideis littoralis 220 8.6 Hargeria rapax 145 12.7 Gammarus tigrinus 93 7.8 Gammarus mucronatus 98 9.2 Apocorophium sp. 260 13.3 TOOLEY CREEK DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Apocorophium lacustre 300 8.9 Gammarus tigrinus 300 18.6 Apocorophium lacustre 201 15.5 Gammarus tigrinus 216 13.5 not monitored - - not monitored - - not monitored - - not monitored - - Littoridinops sp. 300 8.9 Littoridinops sp. 296 18.4 Gammarus tigrinus 201 15.5 Littoridinops sp. 216 13.5 - - - - - - - - - - - - Gammarus tigrinus 300 8.9 Apocorophium lacustre 250 15.5 Littoridinops sp. 189 14.5 Hargeria rapax 208 13.0 - - - - - - - - - - - - Dicrotendipes sp. 300 8.9 Amphicteis fioridus 232 14.4 Amphicteis fioridus 145 11.2 Amphicteis fioridus 141 8.8 - - - - - - - - - - - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - Apocorophium sp. 300 29.9 Apocorophium sp. 239 22.0 Apocorophium sp. 203 18.3 Hargena rapax 224 24.1 Littoridinops tenuipes 250 17.7 - - - - - - - - - Hargena rapax 133 13.2 Hargena rapax 148 13.6 Americamysis almyra 173 15.5 Apocorophium sp. 105 11.3 Apocorophium sp. 241 17.1 - - - - - - - - - Apocorophium louisianum 119 11.8 Apocorophium louisianum 129 11.9 Littoridinops tenuipes 158 14.2 Littoridinops sp. 77 8.3 Hargena rapax 228 16.2 - - - - - - - - - Palaemonetes pugio 75 7.5 Littoridinops sp. 114 10.5 Hargena rapax 155 14.0 Nematoda sp. 71 7.7 Gammarus mucronatus 224 15.9 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Apocorophium sp. 300 13.4 Littoridinops sp. 300 25.0 Littoridinops sp. 300 22.9 Gammarus tigrinus 213 19.9 Littoridinops sp. 300 21.2 Hargena rapax 90 28.0 Littoridinops sp. 200 22.2 Gammarus tigrinus 300 13.4 Gammarus tigrinus 256 21.3 Gammarus tigrinus 267 20.4 Littoridinops sp. 195 18.2 Apocorophium sp. 247 17.4 Littoridinops tenuipes 73 22.7 Hargena rapax 120 13.3 Littoridinops sp. 300 13.4 Apocorophium sp. 208 17.3 Apocorophium sp. 229 17.5 Hargena rapax 164 15.3 Gammarus tigrinus 164 11.6 Gammarus tigrinus 69 21.5 Tanytarsus sp. 102 11.3 Hargena rapax 275 12.3 Tanytarsus sp. 77 6.4 Apocorophium lacustre 115 8.8 Gammarus mucronatus 105 9.8 Apocorophium louisianum 124 8.8 Apocorophium sp. 49 15.3 Gammarus tigrinus 101 11.2 MI MUDDY CREEK (CONTROL) UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus 300 11.9 Gammarus tigrinus 300 22.5 Chironomus decorus 260 13.2 Littoridinops sp. 264 16.2 Littoridinops sp. 300 20.0 Goeldichironomus devineyae 239 28.8 Littoridinops sp. 275 30.8 Gammarus tigrinus 268 17.9 Amphicteis fioridus 292 11.6 Littoridinops sp. 300 22.5 Littoridinops sp. 246 12.5 Gammarus tigrinus 247 15.1 Apedilum sp. 258 17.2 Littoridinops sp. 229 27.6 Gammarus tigrinus 114 12.8 Apocorophium sp. 259 17.3 Apocorophium lacustre 288 11.4 Amphicteis fioridus 191 14.3 Amphicteis fioridus 208 10.6 Corixidae sp. 225 13.8 Goeldichironomus devineyae 230 15.3 Chironomus decorus 176 21.1 Apocorophium sp. 98 11.0 Littoridinops sp. 256 17.1 Littoridinops sp. 256 10.2 Corixidae sp. 106 7.9 Gammarus tigrinus 201 10.2 Amphicteis fioridus 218 13.4 Enchytraeidae sp. 119 7.9 Gammarus tigrinus 45 5.4 Amphicteis fioridus 51 5.7 Amphicteis fioridus 185 12.4 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Chironomus decorus 300 14.1 Apedilum sp. 141 16.0 Goeldichironomus devineyae 248 25.6 Goeldichironomus devineyae 240 19.6 Goeldichironomus devineyae 300 12.7 Littoridinops tenuipes 184 30.6 Goeldichironomus devineyae 300 20.5 Littoridinops tenuipes 272 20.6 Littoridinops sp. 300 14.1 Littoridinops sp. 120 13.6 Littoridinops sp. 190 19.6 Apocorophium sp. 233 19.0 Apocorophium sp. 300 12.7 Apocorophium louisianum 102 16.9 Littoridinops sp. 300 20.5 Chironomus decorus 224 17.0 Apocorophium lacustre 300 14.1 Goeldichironomus devineyae 119 13.5 Naididae sp. 118 12.2 Palaemonetes pugio 131 10.7 Chironomus decorus 300 12.7 Palaemonetes pugio 76 12.6 Chironomus decorus 150 10.2 Apocorophium sp. 168 12.7 Gammarus tigrinus 283 13.3 Glyptotendipes sp. 80 9.1 Apocorophium sp. 111 11.4 Chironomus decorus 119 9.7 Littoridinops sp. 230 9.7 Goeldichironomus devineyae 64 10.6 Gammarus tigrinus 107 7.3 Parachironomus sp. 132 10.0 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Littoridinops sp. 300 15.8 Apocorophium sp. 290 23.7 Apocorophium sp. 300 17.2 Apocorophium sp. 300 15.3 Littoridinops sp. 144 11.6 Gammarus tigrinus 300 19.8 Littoridinops sp. 275 20.9 Apocorophium sp. 256 13.5 Littoridinops sp. 254 20.8 Gammarus tigrinus 300 17.2 Gammarus tigrinus 300 15.3 Candonidae sp. 140 11.3 Apocorophium sp. 258 17.0 Gammarus tigrinus 251 19.1 Tanytarsus sp. 252 13.3 Gammarus tigrinus 216 17.7 Littoridinops sp. 236 13.5 Littoridinops sp. 300 15.3 Gammarus tigrinus 132 10.6 Apocorophium louisianum 199 13.1 Apocorophium sp. 243 18.5 Chironomus sp. 240 12.6 Apocorophium lacustre 114 9.3 Amphicteis fioridus 134 7.7 Chironomus decorus 233 11.9 Apocorophium sp. 98 7.9 Littoridinops tenuipes 182 12.0 Amphicteis fioridus 135 10.3 MUDDY CREEK (CONTROL) DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus 300 9.8 Gammarus tigrinus 300 26.5 Apocorophium lacustre 231 19.5 Littoridinops sp. 232 10.0 Apedilum sp. 228 27.5 Chironomus decorus 209 26.8 Littoridinops sp. 300 17.4 Apocorophium sp. 300 16.0 Amphicteis fioridus 300 9.8 Littoridinops sp. 300 26.5 Gammarus tigrinus 206 17.4 Cricotopus/Orthocladius sp. 41 218 9.4 Littoridinops sp. 223 26.9 Gammarus tigrinus 148 19.0 Tanytarsus sp. 236 13.7 Gammarus tigrinus 300 16.0 Apocorophium lacustre 265 8.7 Apocorophium lacustre 225 19.9 Amphicteis fioridus 155 13.1 Hargena rapax 210 9.1 Hargena rapax 69 8.3 Littoridinops sp. 110 14.1 Gammarus tigrinus 204 11.8 Littoridinops sp. 265 14.2 Littoridinops sp. 239 7.8 Cyrenoida floridana 100 8.8 Littoridinops sp. 135 11.4 Gammarus tigrinus 205 8.9 Goeldichironomus devineyae 53 6.4 Apocorophium sp. 100 12.8 Apocorophium sp. 197 11.4 Cricotopus sp. 184 9.8 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Apocorophium lacustre 300 28.1 Apedilum sp. 152 21.3 Gammarus mucronatus 174 23.1 Apocorophium sp. 281 16.8 Apocorophium sp. 300 15.0 Palaemonetes pugio 142 26.7 Goeldichironomus devineyae 266 18.5 Gammarus mucronatus 300 15.5 Hargena rapax 214 20.0 Naididae sp. w/o hair 77 10.8 Nematoda sp. 128 17.0 Apocorophium louisianum 176 10.5 Gammarus mucronatus 262 13.1 Littoridinops tenuipes 140 26.3 Littoridinops sp. 198 13.8 Littoridinops tenuipes 300 15.5 Gammarus tigrinus 158 14.8 Goeldichironomus devineyae 75 10.5 Hargena rapax 111 14.8 Gammarus tigrinus 174 10.4 Hargena rapax 208 1.4 Apocorophium sp. 104 19.5 Chironomus decorus 190 13.2 Chironomus decorus 300 15.5 Littoridinops sp. 110 10.3 Palaeomonetes pugio 72 10.1 Palaemonetes pugio 62 8.2 Hargena rapax 165 9.9 Goeldichironomus devineyae 182 9.1 Apocorophium louisianum 28 5.3 Gammarus mucronatus 140 9.7 Goeldichironomus devineyae 237 12.2 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Apocorophium sp. 300 16.4 Apocorophium sp. 300 16.0 Gammarus tigrinus 300 16.9 Gammarus tigrinus 300 21.0 Apocorophium sp. 300 20.4 Gammarus tigrinus 276 35.2 Littoridinops sp. 259 28.8 Littoridinops sp. 300 16.4 Gammarus tigrinus 300 16.0 Littoridinops sp. 300 16.9 Littoridinops sp. 300 21.0 Littoridinops sp. 300 20.4 Littoridinops tenuipes 242 30.9 Gammarus tigrinus 191 21.2 Gammarus tigrinus 240 7.9 Littoridinops sp. 298 15.9 Apocorophium sp. 194 10.9 Apocorophium sp. 295 20.6 Apocorophium louisianum 205 14.0 Apocorophium sp. 66 8.4 Apocorophium sp. 160 17.8 Gammarus mucronatus 145 7.5 Apocorophium louisianum 254 13.6 Tanytarsus sp. 132 7.4 Apocorophium louisianum 150 10.5 Gammarus tigrinus 190 12.9 Corixidae sp. 42 5.4 Palaemonetes pugio 88 9.8 III-G-80 Table III-G2 (continued). M 2 0 W w Ce H O Cr) 0 �. > > = I- 2- M Q 0 W Cr) I- J Cr) 0 = 0 0 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - - - - - - - - - - Gammarus tigrinus Littoridinops sp. Palaeomonetes pugio Amphicteis fioridus 292 241 211 108 28.1 23.2 20.3 10.4 Littoridinops sp. Palaeomonetes pugio Naididae sp. Cyprideis littoralis 147 68 47 30 40.2 18.6 12.8 8.2 Cyprideis littoralis Littoridinops sp. Naididae sp. Palaeomonetes pugio 247 208 144 130 24.8 20.9 14.5 13.1 not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Cyprideis littoralis Littoridinops sp. Palaeomonetes pugio Naididae sp. w/o hair 267 162 102 31 40.5 24.5 15.5 4.7 Palaeomonetes pugio Cyprideis littoralis Gammarus tigrinus Amphicteis fioridus 227 117 24 24 48.7 25.1 5.2 5.2 Palaemonetes pugio Cyprideis littoralis Naididae sp. Apocorophium sp. 133 43 18 11 50.0 16.2 6.8 4.1 Apocorophium sp. Chironomus decorus Amphicteis floridus Palaemonetes pugio 176 147 136 79 26.0 21.7 20.1 11.7 Cyprideis littoralis Palaemonetes pugio Amphicteis floridus Apocorophium sp. 292 149 40 32 46.0 23.5 6.3 5.0 Palaemonetes pugio Cyprideis littoralis Mediomastus amhiseta Strehlospio benedicti 111 43 23 14 40.8 15.8 8.5 5.1 Cyprideis littoralis Amphicteis fioridus Palaemonetes pugio Goeldichironomus devineyae 114 43 30 25 31.7 11.9 8.3 6.9 Palaemonetes pugio Cyprideis littoralis Chironomus decorus Goeldichironomus devineyae 191 59 49 31 39.0 12.0 10.0 6.3 2015 2016 2017 2018 2019 2020 2021 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Littoridinops sp. Tanytarsus sp. Cyprideis littoralis Amphicteis fioridus 1998 247 216 200 109 22.3 19.5 18.0 9.8 Cyprideis littoralis Tubificoides heterochaetus Palaemonetes pugio Tanytarsus sp. 1999 300 237 186 179 18.2 14.4 11.3 10.9 Cyprideis littoralis Littoridinops sp. Apocorophium sp. Palaemonetes pugio 2000 300 183 110 67 34.8 21.2 12.8 7.8 Cyprideis littoralis Palaemonetes pugio Apocorophium sp. Littoridinops sp. 2001 116 79 78 63 17.3 11.8 11.6 9.4 Littoridinops sp. Palaemonetes pugio Cyprideis littoralis Chironomus decorus 2002 221 144 121 94 24.7 16.1 13.5 10.5 Paleomonetes pugio Mediomastus ambiseta Tubificoides heterochaetus Strehlospio benedicti 2003 228 31 27 23 60.0 8.2 7.1 6.1 Chironomus decorus Tanytarsus sp. Amphicteis fioridus Apocorophium sp. 2004 75 28 27 24 34.2 12.8 12.3 11.0 lli Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - - - - - - - - - Littoridinops sp. Gammarus tigrinus Amphicteis fioridus Edotia montosa 68 59 20 17 26.6 23.0 7.8 6.6 Palaeomonetes pugio Apocorophium Iacustre Shrimp zoea Gammarus tigrinus 128 123 70 59 24.3 23.3 13.3 11.2 Littoridinops sp. Amphicteis fioridus Apocorophium Iacustre Naididae sp. 80 29 26 10 40.2 14.6 13.1 5.0 not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus Palaeomonetes pugio Apocorophium Iacustre Rhithropanopeus harrisii 173 89 62 14 41.0 21.1 14.7 3.3 Polydora cornuta Gammarus tigrinus Palaeomonetes pugio Neanthes succinea 117 75 62 35 29.4 18.8 15.6 8.8 Gammarus tigrinus Palaemonetes pugio Gammarus mucronatus Mulinia lateralis 105 51 38 19 36.1 17.5 13.1 6.5 Apocorophium sp. Gammarus tigrinus Palaemonetes pugio Apocorophium Iacustre 114 25 19 16 60.3 13.2 10.1 8.4 Palaemonetes pugio Gammarus tigrinus Apocorophium sp. Nematoda sp. 33 16 7 6 44.0 21.3 9.3 8.0 Palaemonetes pugio Gammarus mucronatus Neanthes succinea Gammarus tigrinus 166 77 59 27 41.2 19.1 14.6 6.7 Gammarus tigrinus Gammarus mucronatus Palaemonetes pugio Balanus improvisus 262 138 64 10 52.2 27.5 12.7 2.0 Gammarus tigrinus Palaemonetes pugio Goeldichironomus devineyae Apocorophium sp. 251 28 22 12 67.0 7.5 5.9 3.2 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus Apocorophium sp. Littoridinops sp. Palaeomonetes pugio 206 83 63 48 45.9 18.5 14.0 10.7 Gammarus tigrinus Apocorophium sp. Palaeomonetes pugio Apocorophium Iacustre 300 107 104 38 44.2 15.8 15.3 5.6 Gammarus tigrinus Apocorophium sp. Apocorophium Iacustre Littoridinops sp. 214 213 146 131 20.2 20.2 13.8 12.4 Gammarus tigrinus Balanus improvisus Gammarus mucronatus Palaeomonetes pugio 300 91 83 41 49.8 15.1 13.8 6.8 Palaeomonetes pugio Gammarus tigrinus Littoridinops sp. Chironomus decorus 150 109 18 4 49.3 35.9 5.9 1.3 Gammarus tigrinus Paleomonetes pugio Lepidactylus dytiscus Gammarus mucronatus 270 135 30 23 53.4 26.7 5.9 4.5 Gammarus tigrinus Apocorophium sp. Cricotopus sp. Dicrotendipes nervosus 90 8 7 7 63.8 5.7 5.0 5.0 PORTER CREEK UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - Apocorophium sp. Gammarus tigrinus Littoridinops sp. Dicrotendipes nervosus 300 300 252 204 12.9 12.9 10.9 8.8 Gammarus tigrinus Candonidae sp. Littoridinops tenuipes Corixidae sp. 300 300 294 290 10.7 10.7 10.5 10.3 Gammarus tigrinus Littoridinops sp. Apocorophium sp. Amphicteis fioridus 300 300 250 139 21.8 21.8 18.2 10.1 Gammarus tigrinus Littoridinops tenuipes Amphicteis fioridus Cyprideis littoralis 300 261 242 215 16.1 14.0 13.0 12.0 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus Cyprideis littoralis Amphicteis fioridus Cyathura polka 300 292 236 132 24.8 24.2 19.5 10.9 Gammarus tigrinus Cyprideis littoralis Americamysis almyra Amphicteis fioridus 300 130 105 59 39.2 17.0 13.7 7.7 Gammarus tigrinus Littoridinops sp. Cyprideis littoralis Amphicteis fioridus 300 258 162 91 28.8 24.8 15.6 8.8 Gammarus tigrinus Littoridinops sp. Apocorophium Iacustre Cyprideis littoralis 300 183 129 112 30.1 18.4 13.0 11.2 Littoridinops sp. Gammarus tigrinus Naididae sp. w/o hair Cyprideis littoralis 249 184 152 109 20.9 15.4 12.7 9.1 Apocorophium sp. Gammarus tigrinus Littoridinops tenuipes Apocorophium Iouisianum 300 300 300 202 19.0 19.0 19.0 12.8 Gammarus tigrinus Littoridinops sp. Amphicteis fioridus Goeldichironomus devineyae 263 244 115 40 31.6 29.3 13.8 4.8 PORTER CREEK DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - Apocorophium Iacustre Gammarus tigrinus Apocorophium Iouisianum Littoridinops sp. 300 300 202 178 16.8 16.8 11.3 10.0 Littoridinops tenuipes Apedilum sp. Hargeria rapax Apocorophium Iouisianum 238 88 73 51 35.8 13.2 11.0 7.7 Hargeria rapax Gammarus tigrinus Apocorophium sp. Gammarus mucronatus 300 298 153 152 23.4 23.2 11.9 11.8 Apocorophium sp. Hargeria rapax Gammarus mucronatus Littoridinops tenuipes 300 254 228 220 19.0 16.1 14.4 13.9 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus Americamysis almyra Littoridinops sp. Palaeomonetes pugio 300 171 153 78 28.1 16.0 14.3 7.3 Gammarus tigrinus Apocorophium sp. Littoridinops sp. Apocorophium Iacustre 300 212 174 76 35.5 25.1 20.6 9.0 Apocorophium sp. Gammarus tigrinus Apocorophium Iacustre Littoridinops sp. 300 300 242 224 25.1 25.1 20.3 18.8 Gammarus tigrinus Littoridinops sp. Gammarus mucronatus Apocorophium sp. 300 242 120 100 28.1 22.7 11.2 9.4 Littoridinops sp. Gammarus tigrinus Apocorophium sp. Cassidinidea lunifrons 250 122 107 93 33.4 16.3 14.3 12.4 Gammarus tigrinus Apocorophium sp. Hargeria rapax Littoridinops tenuipes 252 232 197 160 23.0 21.2 18.0 14.6 Littoridinops sp. Balanus subalbidus Paleomonetes pugio Gammarus tigrinus 89 25 24 22 49.4 13.9 13.3 12.2 III-G-81 Table III-G2 (continued). 2 .- W ct M H 0 d 2 Wnot .- Ct M 0 Z pDominant 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - Gammarus tignnus Littondinops sp. Amphicteis flondus Berosus sp. 300 300 238 181 21.2 21.2 16.8 12.8 Gammarus tignnus Littondinops tenuipes Gyprideis littoralis Dicrotendipes nervosus 300 300 223 156 17.2 17.2 12.8 9.0 2015 2016 2017 2018 2019 2020 2021 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gyprideis littoralis Gammarus tignnus Littondinops sp. Dicrotendipes nervosus 1998 300 300 300 166 17.1 17.1 17.1 9.5 Gammarus tignnus Littondinops sp. Tanytarsus sp. Gyprideis littoralis 292 208 108 88 29.0 20.6 10.7 8.7 Gyprideis littoralis Gammarus tignnus Littondinops sp. Cassidinidea lunifrons 300 300 300 82 26.7 26.7 26.7 7.3 Gyprideis littoralis Gammarus tignnus Littondinops sp. Amphicteis flondus 2001 300 300 300 218 19.3 19.3 19.3 14.0 Dicrotendipes nervosus Dero sp. Gammarus tigrinus Tanytarsus sp. 2002 132 67 67 63 21.5 10.9 10.9 10.3 Apocorophium sp. Gammarus tignnus Littondinops tenuipes Gyprideis littoralis 2003 300 300 300 244 17.9 17.9 17.9 14.5 Dicrotendipes nervosus Littondinops sp. Tanytarsus sp. Amphicteis flondus 2004 102 95 66 47 24.2 22.6 15.7 11.2 1999 2000 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - Apocorophium lacustre Gammarus tignnus Littondinops sp. Apocorophium sp. 300 300 300 292 15.2 15.2 15.2 14.8 Gammarus tignnus Littondinops tenuipes Tanytarsus sp. Cyprideis littoralis 300 300 268 217 15.9 15.9 14.2 11.5 2015 2016 2017 2018 2019 2020 2021 Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Cyprideis littoralis Dicrotendipes nervosus Gammarus tignnus Littondinops sp. 300 300 300 300 13.6 13.6 13.6 13.6 Gammarus tignnus Littondinops sp. Cyprideis littoralis Tanytarsus sp. 272 181 176 134 20.9 13.9 13.5 10.3 Cyprideis littoralis Littondinops sp. Gammarus tignnus Tanytarsus sp. 300 300 278 106 24.1 24.1 22.3 8.5 Cyprideis littoralis Gammarus tignnus Littondinops sp. Tanytarsus sp. 300 300 300 164 22.2 22.2 22.2 12.2 Dicrotendipes nervosus Littondinops sp. Gammarus tignnus Tanytarsus sp. 194 147 145 91 22.4 17.0 16.7 10.5 Littondinops tenuipes Tanytarsus sp. Gammarus tignnus Apocorophium sp. 300 300 284 230 15.8 15.8 15.0 12.1 Dicrotendipes nervosus Cricotopus sp. Littondinops sp. Tanytarsus sp. 141 60 59 39 37.7 16.0 15.8 10.4 DCUT19 (CONTROL) UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - Littondinops sp. Gammarus tignnus Amphicteis flondus Chironomus decorus 300 142 139 132 23.7 11.2 11.0 10.4 Littondinops tenuipes Apocorophium sp. Nematoda sp. Amphicteis flondus 300 250 197 187 15.0 12.4 9.8 9.3 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Littondinops sp. Tanytarsus sp. Gammarus tignnus Cypndeis &torahs 266 107 92 86 32.3 13.0 11.2 10.4 Cypndeis littoralis Littondinops sp. Tanytarsus sp. Goeldichironomus devineyae 300 280 276 95 21.1 19.7 19.5 6.7 Cypndeis littoralis Littondinops sp. Gammarus tignnus Amphicteis flondus 300 300 250 122 18.3 18.3 15.3 7.5 Apocorophium sp. Littondinops sp. Gammarus tignnus Candonidae sp. 300 300 227 180 21.6 21.6 16.3 12.9 Tanytarsus sp. Littondinops sp. Dero sp. Dicrotendipes nervosus 274 258 190 108 24.7 23.3 17.1 9.7 Apocorophium sp. Littondinops tenuipes Berosus sp. Apocorophium louisianum 300 300 165 105 26.0 26.0 14.3 9.1 Littondinops sp. Goeldichironomus devineyae Tanytarsus sp. Dicrotendipes nervosus 300 81 78 52 44.5 12.0 11.6 7.7 DCUT19 (CONTROL) DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - Littondinops sp. Gammarus tignnus Hargena rapax Nematoda sp. 251 232 222 208 10.2 9.4 9.0 8.4 Littondinops tenuipes Hargena rapax Apocorophium sp. Goeldichironomus devineyae 300 287 215 215 12.3 11.8 8.8 8.8 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Littondinops sp. Tanytarsus sp. Cypndeis littoralis Gammarus tignnus 300 300 236 221 13.1 13.1 10.3 9.6 Littondinops sp. Gammarus tignnus Cypndeis littoralis Tanytarsus sp. 300 199 156 141 18.1 12.0 9.4 8.5 Gammarus tignnus Littondinops sp. Cypndeis littoralis Tanytarsus sp. 300 300 293 205 17.0 17.0 16.6 11.6 Gammarus tignnus Littondinops sp. Apocorophium sp. Hargena rapax 300 252 223 66 25.3 21.3 18.8 5.6 Littondinops sp. Gammarus tignnus Tanytarsus sp. Dicrotendipes nervosus 300 214 127 117 25.7 18.4 10.9 10.0 Apocorophium sp. Littondinops tenuipes Gammarus tignnus Hargena rapax 300 300 286 218 18.1 18.1 17.2 13.1 Littondinops sp. Palaemonetes pugio Amphicteis floridus Chironomus decorus 156 81 64 51 34.2 17.8 14.0 11.2 III-G-82 Table III-G2 (concluded). DUCK CREEK (CONTROL) UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Gammarus tignnus 164 17.4 Littoridinops tenuipes 252 22.0 Littoridinops sp. 225 35.9 Littoridinops tenuipes 300 19.4 Littoridinops sp. 123 13.1 Tanytarsus sp. 217 19.0 Cyprideis littoralis 79 12.6 Gammarus tignnus 300 19.4 Dicrotendipes nervosus 119 12.6 Cyprideis littoralis 179 15.6 Tanytarsus sp. 77 12.2 Chironomus decorus 300 19.4 Tanytarsus sp. 96 10.2 Gammarus tignnus 153 13.4 Gammarus tignnus 67 10.7 Tanytarsus sp. 203 13.2 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Dicrotendipes nervosus 300 15.3 Gammarus tignnus 300 17.6 Gammarus tignnus 300 20.3 Gammarus tignnus 300 20.7 Dero sp. 269 15.8 Cyprideis littoralis 300 31.1 Gammarus tignnus 300 22.1 Gammarus tignnus 300 15.3 Littoridinops sp. 300 17.6 Cyprideis littoralis 255 17.3 Paranais litoralis 272 18.8 Pristina sp. 233 13.7 Littoridinops tenuipes 300 31.1 Dicrotendipes nervosus 282 20.8 Tanytarsus sp. 300 15.3 Naididae sp. w/o hair 300 17.6 Littoridinops sp. 195 13.2 Cyprideis littoralis 251 17.3 Gammarus tignnus 186 10.9 Gammarus tignnus 211 21.8 Littoridinops sp. 227 16.7 Cyprideis littoralis 240 12.3 Cyprideis littoralis 234 13.7 Naididae sp. w/o hair 168 11.4 Littoridinops sp. 194 13.4 Kiefferulus sp. 162 9.5 Amphicteis flondus 31 3.2 Tanytarsus sp. 173 12.7 J DUCK CREEK (CONTROL) DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Apocorophium sp. 300 14.5 Littoridinops tenuipes 300 27.7 Gammarus tignnus 300 21.5 Gammarus tignnus 300 13.8 Apocorophium lacustre 290 14.0 Hargeria rapax 209 19.3 Hargeria rapax 285 20.4 Hargeria rapax 300 13.8 Hargeria rapax 274 13.3 Apocorophium sp. 107 9.9 Littoridinops sp. 240 17.2 Apocorophium sp. 290 13.4 Gammarus tignnus 236 11.4 Apedilum sp. 91 8.4 Gammarus mucronatus 139 9.9 Littoridinops tenuipes 227 10.5 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tignnus 290 24.3 Gammarus tignnus 300 25.5 Apocorophium sp. 300 22.8 Gammarus tignnus 300 24.3 Gammarus tignnus 300 33.7 Apocorophium sp. 290 35.8 Palaemonetes pugio 156 27.2 Littoridinops sp. 263 22.1 Apocorophium lacustre 217 18.5 Gammarus tignnus 300 22.8 Littoridinops sp. 300 24.3 Littoridinops sp. 189 21.2 Gammarus tignnus 177 21.9 Gammarus tignnus 151 26.3 Cyprideis littoralis 247 20.7 Apocorophium sp. 206 17.5 Littoridinops sp. 261 19.8 Hargeria rapax 122 9.9 Palaemonetes pugio 115 12.9 Littoridinops tenuipes 141 17.4 Dicrotendipes nervosus 71 12.4 Dicrotendipes nervosus 75 6.3 Littoridinops sp. 159 13.5 Apocorophium lacustre 237 18.0 Goeldichironomus devineyae 107 8.6 Amphicteis flondus 64 7.2 Paleomonetes pugio 93 11.5 Tanytarsus sp. 44 7.7 III-G-83 Table III-G3. Benthic macroinvertebrate community structure data for sweep samples collected at upstream and downstream stations in May during years 1998-2005 and 2007-2021. A "-" indicates the creek was not monitored that year. Pre- and post -Mod Alt L collection years for impact creeks are separated by bold vertical lines. Benthic collection occurred in June for 2010. Summary pre- and post -Mod Alt L averages are shown in Table II-05. An "a" refers to only those taxa for which sensitivity values are available were used in EBI calculations. Note: sensitivity values provided by NCDWR. 1998 1999 2000 2001 2002 2003 2004 2005 2007 Station community structure data by year 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 PA2 (CONTROL) JACOBS CREEK JACKS CREEK SCUT1 (CONTROL) BROOMFIELD SWAMP CREEK Total abundance - - - - - - - - - - - - - - - - - - - - 293 809 264 Q Total taxa - - - - - - - - - - - - - - - - - - - - 30 16 26 D Total taxa with sensitivity values - - - - - - - - - - - - - - - - - - - - 21 11 18 EBIa - - - - - - - - - - - - - - - - - - - - 1.46 1.54 1.31 Total abundance - - - - - - - - - - - - - - - - - - - - 745 821 380 I Total taxa - - - - - - - - - - - - - - - - - - - - 41 17 29 o Total taxa with sensitivity values - - - - - - - - - - - - - - - - - - - - 28 14 22 EBIa - - - - - - - - - - - - - - - - - - - - 1.34 1.64 1.48 Total abundance - - - - - - - - - - - - - - - - - - - - 401 944 579 Q Total taxa - - - - - - - - - - - - - - - - - - - - 28 20 28 D Total taxa with sensitivity values - - - - - - - - - - - - - - - - - - - - 21 12 17 EBIa - - - - - - - - - - - - - - - - - - - - 1.18 1.53 1.25 Total abundance - - - - - - - - - - - - - - - - - - - - 738 997 446 I Total taxa - - - - - - - - - - - - - - - - - - - - 35 18 28 o Total taxa with sensitivity values - - - - - - - - - - - - - - - - - - - - 25 15 20 EBIa - - - - - - - - - - - - - - - - - - - - 1.72 1.98 1.83 Total abundance 2,654 783 2,430 2,823 987 1,190 2,223 1,925 - - - - 3,529 865 1,771 1,090 1,587 1,603 1,311 2,144 2,480 1,647 901 Q Totaltaxa 42 18 34 24 30 24 39 29 - - - - 35 19 29 33 30 35 33 30 37 27 31 D Total taxa with sensitivity values 34 13 27 19 25 19 34 25 - - - - 26 15 21 24 25 24 24 25 24 21 26 EBIa 1.78 1.97 1.74 1.86 1.75 1.72 1.90 1.99 - - - - 2.00 1.99 2.07 1.59 1.90 1.83 1.77 1.74 1.69 1.83 1.92 Total abundance 2,477 995 589 2,053 829 781 1,014 1,379 - - - - 2,016 808 880 1,599 1,565 1,551 766 1,136 2,189 1,334 304 I Totaltaxa 45 19 25 25 32 28 30 29 - - - - 33 26 27 33 31 31 24 32 33 23 19 o Total taxa with sensitivity values 35 16 22 23 28 24 27 25 - - - - 27 24 24 27 28 27 20 28 27 19 15 EBIa 1.77 2.05 1.77 1.85 1.93 2.04 2.19 2.05 - - - - 2.03 1.93 2.02 1.86 2.01 2.00 2.04 2.02 1.74 1.93 1.98 Total abundance - - - - - - - - - - - - 1,678 937 1,560 1,248 1,644 1,582 1,388 1,267 1,190 718 792 Q Total taxa - - - - - - - - - - - - 32 19 24 28 26 34 25 21 32 24 27 D Total taxa with sensitivity values - - - - - - - - - - - - 25 16 18 19 20 23 22 17 25 20 22 EBIa - - - - - - - - - - - - 2.05 2.40 2.11 1.79 1.85 1.92 1.63 1.79 1.83 1.85 1.92 Total abundance - - - - - - - - - - - - 1,523 1,024 1,237 2,145 1,565 1,596 1,409 2,198 1,420 905 890 1 0 Total taxa - - - - - - - - - - - - 33 32 29 35 35 29 32 30 34 30 32 o Total taxa with sensitivity values - - - - - - - - - - - - 26 27 22 30 29 23 27 28 32 27 26 EBIa - - - - - - - - - - - - 2.10 2.06 2.05 1.86 2.01 1.98 1.80 1.91 1.91 1.98 1.85 Total abundance - - - - - - - - - - - - 1,916 1,504 1,312 977 1,789 2,040 1,684 1,292 2,004 1,270 1,121 Q Total taxa - - - - - - - - - - - - 26 27 23 28 30 27 26 24 31 19 27 D Total taxa with sensitivity values - - - - - - - - - - - - 21 19 14 22 22 20 22 21 22 17 23 EBIa - - - - - - - - - - - - 2.09 2.23 2.02 1.71 1.81 1.72 1.81 1.85 1.70 1.86 1.74 Total abundance - - - - - - - - - - - - 1,502 1,330 1,263 1,123 1,628 1,768 1,325 2,183 1,996 1,320 1,132 I Total taxa - - - - - - - - - - - - 28 22 21 30 29 27 29 30 31 26 30 o Total taxa with sensitivity values - - - - - - - - - - - - 20 16 17 22 24 22 24 26 23 23 26 EBIa - - - - - - - - - - - - 2.22 2.33 1.82 1.77 1.84 1.76 1.79 1.84 1.83 1.84 1.75 III-G-84 Table III-G3 (continued). Station data by year 1998 1999 2000 2001 2002 2003 2004 2005 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 HUDDLES CUT MUDDY CREEK TOOLEY CREEK LONG CREEK LITTLE CREEK DRINKWATER CREEK (CONTROL) (CONTROL) (CONTROL) 0_ D Total abundance Total taxa Total taxa with sensitivity values E Bla - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2,032 34 28 2.25 1,026 28 22 1.96 1,670 26 21 1.93 1,212 28 22 1.75 1,457 22 20 1.98 1,661 31 24 1.86 1,484 26 23 1.94 1,777 26 25 1.87 596 30 25 1.87 881 22 18 1.82 1,250 28 23 1.82 Total abundance - - - - - - - - - - - - 1,330 981 1,248 1,285 1,785 1,408 1,337 1,513 884 1,001 970 Total taxa - - - - - - - - - - - - 38 33 32 27 32 40 30 30 26 24 25 0 o Total taxa with sensitivity values - - - - - - - - - - - - 32 29 26 20 27 31 25 26 22 21 19 E Bla - - - - - - - - - - - - 2.04 1.78 1.90 1.88 1.87 1.88 1.89 1.86 2.04 1.73 1.76 Total abundance - - - - - - - - - - - - 1,148 1,238 2,002 1,197 961 1,017 1,279 1,032 1,204 881 404 0_ Total taxa - - - - - - - - - - - - 22 27 22 26 25 27 29 27 24 15 29 D Total taxa with sensitivity values - - - - - - - - - - - - 20 19 16 21 17 21 22 23 19 14 20 E Bla - - - - - - - - - - - - 1.77 1.92 1.77 1.58 1.56 1.68 1.57 1.62 1.50 1.79 1.42 Total abundance - - - - - - - - - - - - 1,363 843 1,152 1,005 1,292 1,324 1,502 564 611 1,430 59 Total taxa - - - - - - - - - - - - 33 36 28 31 33 26 32 21 36 32 16 0 o Total taxa with sensitivity values - - - - - - - - - - - - 22 30 24 26 28 21 27 17 26 28 14 E Bla - - - - - - - - - - - - 1.99 2.02 2.25 1.84 2.05 1.80 1.79 2.08 1.94 1.83 2.04 Total abundance - - - - - - - - - - - - 1,588 740 974 1,612 2,358 1,542 1,445 1,424 2,930 250 1,292 0_ Total taxa - - - - - - - - - - - - 33 29 23 34 31 26 31 32 40 21 24 D Total taxa with sensitivity values - - - - - - - - - - - - 27 25 20 29 23 23 24 28 28 13 20 E Bla - - - - - - - - - - - - 2.05 1.93 1.70 1.86 1.86 1.99 1.88 1.79 1.64 2.03 1.84 Total abundance - - - - - - - - - - - - 892 1,109 777 1,354 1,406 1,169 1,566 959 1,023 530 672 0 Total taxa - - - - - - - - - - - - 24 30 29 33 37 28 28 27 23 17 26 o Total taxa with sensitivity values - - - - - - - - - - - - 23 23 24 26 29 23 26 23 22 16 23 E Bla - - - - - - - - - - - - 2.04 1.98 1.97 2.03 1.97 2.12 1.99 2.01 1.91 2.09 1.86 Total abundance 1,809 1,148 2,148 2,276 - - - - - - - 918 1,587 1,138 1,630 1,928 2,448 1,936 2,572 1,143 1,196 1,061 1,962 0_ Total taxa 42 17 33 33 - - - - - - - 18 31 27 27 28 31 30 36 30 32 28 24 D Total taxa with sensitivity values 34 14 23 29 - - - - - - - 14 22 21 23 25 26 25 31 28 28 26 20 E Bla 1.92 2.02 1.82 2.08 - - - - - - - 1.92 1.99 2.08 1.82 1.81 1.80 2.01 1.85 1.80 1.91 2.01 1.95 Total abundance 3,360 1,616 1,302 1,595 - - - - - - - 1,005 1,085 1,110 928 1,410 2,425 1,202 1,309 1,072 1,417 321 899 Totaltaxa 47 33 31 34 - - - - - - - 33 36 32 32 34 31 31 31 24 32 18 22 0 o Total taxa with sensitivity values 39 28 27 31 - - - - - - - 28 32 26 28 27 26 27 30 22 28 17 21 E Bla 1.81 1.93 1.90 1.90 - - - - - - - 1.96 1.92 2.04 1.90 2.07 1.94 2.03 1.98 2.01 1.80 1.98 1.92 Total abundance 2,521 1,346 1,971 1,633 1,499 831 899 1,494 2,134 882 970 1,224 2,370 602 1,468 1,419 1,964 1,222 1,744 1,961 1,242 1,517 1,314 0_ Total taxa 32 29 30 38 33 26 36 39 31 27 31 23 28 27 30 29 31 33 34 39 40 34 24 D Total taxa with sensitivity values 25 24 18 30 28 22 27 30 23 22 25 17 20 23 23 22 23 23 30 31 28 29 18 E Bla 1.67 1.85 1.70 1.86 2.01 1.78 2.02 2.01 1.89 2.42 1.72 1.89 1.86 2.05 1.86 1.72 1.91 2.03 1.79 1.84 1.75 2.02 2.00 Total abundance 3,062 1,131 1,183 2,318 833 779 1,722 1,871 1,069 712 751 1,669 1,997 532 1,438 1,939 1,960 1,872 1,780 1,430 1,469 784 900 Total taxa 50 33 32 37 35 26 27 34 27 31 33 30 32 27 25 34 32 30 32 31 29 29 23 0 o Total taxa with sensitivity values 41 27 23 32 28 22 23 29 22 26 29 25 25 20 19 27 29 24 26 28 25 24 19 E Bla 1.78 2.00 1.94 1.77 2.42 1.88 2.05 1.80 1.95 2.47 2.00 1.99 1.98 2.15 1.88 1.87 1.93 2.09 1.87 1.95 1.95 1.93 2.01 Total abundance - 1,040 366 995 - - - - 661 468 277 677 635 273 360 490 1,109 1,647 862 670 896 380 219 0_ Total taxa - 25 17 22 - - - - 20 19 13 15 22 23 18 18 25 25 25 29 26 18 17 D Total taxa with sensitivity values - 18 12 17 - - - - 15 15 10 13 19 20 15 15 19 19 22 26 21 16 14 E Bla - 1.96 1.93 1.91 - - - - 2.07 2.11 2.22 1.81 2.13 2.09 1.86 2.01 1.98 1.96 2.02 2.00 2.03 2.09 1.72 Total abundance - 254 527 206 - - - - 422 398 288 189 75 403 502 375 449 679 1,057 603 304 506 141 Totaltaxa - 24 18 19 - - - - 22 23 26 13 12 20 14 24 22 24 33 26 16 22 15 0 o Total taxa with sensitivity values - 19 14 18 - - - - 19 20 23 13 10 18 12 18 18 22 28 22 11 20 15 E Bla - 2.15 2.20 1.94 - - - - 2.03 1.74 2.08 2.19 2.26 2.26 2.19 1.99 2.11 2.17 2.07 2.11 2.18 2.36 1.93 III-G-85 Table III-G3 (continued). Station data by year 1998 1999 2000 2001 2002 2003 2004 2005 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 DUCK CREEK DCUT19 (CONTROL) DCUT11 PORTER CREEK (CONTROL) 0_ D Total abundance Total taxa Total taxa with sensitivity values E Bla - - - - - - - - - - - - 2,320 - - - - - - - - - - - - 23 - - - - - - - - - - - - 21 - - - - - - - - - - - - 1.85 2,804 34 27 1.88 1,375 22 19 2.05 1,862 31 25 1.87 1,208 21 17 1.87 765 26 21 2.00 1,040 33 23 1.86 996 29 21 2.03 1,193 33 28 1.59 1,578 21 18 2.01 832 33 29 1.86 Total abundance - - - - - - - - - - - - 1,791 667 1,284 1,583 1,068 846 1,194 1,068 749 1,095 180 Total taxa - - - - - - - - - - - - 32 28 33 31 32 26 25 18 25 21 16 0 o Total taxa with sensitivity values - - - - - - - - - - - - 26 24 28 25 26 19 21 18 22 18 11 E Bla - - - - - - - - - - - - 2.07 2.18 2.04 2.01 2.03 2.06 2.07 2.08 2.00 1.82 2.06 Total abundance - - - - - - - - - - - - - - 1,413 1,748 1,750 1,008 1,123 1,553 613 1,677 421 0_ Total taxa - - - - - - - - - - - - - - 24 28 29 22 23 28 30 25 29 D Total taxa with sensitivity values - - - - - - - - - - - - - - 19 22 21 17 15 23 24 21 22 E Bla - - - - - - - - - - - - - - 1.90 1.79 1.75 1.85 1.89 1.97 1.46 2.07 1.52 Total abundance - - - - - - - - - - - - - - 1,970 1,890 2,205 1,229 1,246 1,349 867 1,896 374 Total taxa - - - - - - - - - - - - - - 29 27 31 31 24 19 32 28 24 0 o Total taxa with sensitivity values - - - - - - - - - - - - - - 23 23 26 21 17 16 24 22 19 EBIa - - - - - - - - - - - - - - 2.03 1.86 1.80 1.87 1.98 2.03 1.65 2.00 1.50 Total abundance - - - - - - - - - - - - - - 1,266 2,011 823 1,419 1,636 1,390 1,108 1,152 674 0_ Total taxa - - - - - - - - - - - - - - 23 28 30 33 30 25 25 26 24 D Total taxa with sensitivity values - - - - - - - - - - - - - - 19 24 22 24 21 22 17 22 19 E Bla - - - - - - - - - - - - - - 1.81 1.84 1.87 1.78 1.83 2.00 1.54 1.88 1.84 Total abundance - - - - - - - - - - - - - - 2,471 2,431 2,298 1,653 1,760 1,184 1,166 1,660 456 3 Total taxa - - - - - - - - - - - - - - 32 35 34 34 29 26 32 25 23 0 o Total taxa with sensitivity values - - - - - - - - - - - - - - 26 27 28 27 25 24 25 21 19 E Bla - - - - - - - - - - - - - - 1.90 1.97 1.79 1.74 1.87 1.99 1.81 1.93 1.88 Total abundance - - - - - - - - - - - - 941 1,144 627 1,543 1,956 1,702 1,475 1,450 1,700 966 1,357 0_ Total taxa - - - - - - - - - - - - 30 21 25 21 26 27 34 27 29 22 19 D Total taxa with sensitivity values - - - - - - - - - - - - 24 17 21 18 18 21 23 21 21 18 16 E Bla - - - - - - - - - - - - 1.73 1.85 1.92 1.69 1.64 1.74 1.53 1.52 1.50 1.92 1.64 Total abundance - - - - - - - - - - - - 2,066 1,082 1,397 2,172 1,191 1,175 1,315 1,237 890 809 574 3 Total taxa - - - - - - - - - - - - 26 26 32 28 27 20 23 32 24 22 24 0 o Total taxa with sensitivity values - - - - - - - - - - - - 22 22 28 22 22 17 19 28 20 15 19 E Bla - - - - - - - - - - - - 1.90 2.02 1.91 1.97 1.96 2.13 2.08 1.89 2.01 2.07 2.03 III-G-86 Table III-G3 (concluded). In the sweep summary table below, bold values denote the highest value in a given row (12 bold values total) and bold italics denote the highest value on an upstream or downstream basis (six bold italic values total- three upstream and three downstream). Summary sweep community structure data by year, location (upstream or downstream), and parameter Ranges All creeks (up) 1998 1999 2000 2001 2002 2003 2004 2005 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 Total abundance 1,809 - 2,654 783 - 1,346 366 - 2,430 995 - 2,823 987 - 1,499 831 - 1,190 899 - 2,223 1,494 - 1,925 661 - 2,134 468 - 882 277 - 970 677 - 1,224 635 -3,529 273 - 2,804 360 - 2,002 490 - 2,011 823 - 2,448 765 - 2,040 862 - 2,572 670 - 2,144 293 - 2,930 250-1,677 219-1,962 Total taxa 32 - 42 17 - 29 17 - 34 22 - 38 30 - 33 24 - 26 36 -39 29 - 39 20 - 31 19 - 27 13 - 31 15 -23 22 -35 19 -34 18 -30 18 - 34 21 - 31 22 - 35 23 - 36 21 - 39 24 - 40 15-34 17-33 EBI 1.67- 1.92 1.85-2.02 1.70- 1.93 1.86-2.08 1.75-2.01 1.72- 1.78 1.90-2.02 1.99-2.01 1.89-2.07 2.11 -2.42 1.72-2.22 1.81 - 1.92 1.73-2.25 1.85-2.40 1.70-2.11 1.58-2.01 1.56- 1.98 1.68-2.03 1.53-2.02 1.52-2.03 1.18-2.03 1.53-2.09 1.25-2.00 All creeks (down) Total abundance 2,477-3,360 254 - 1,616 527 - 1,302 206 - 2,318 829 - 833 779 - 781 1,014 - 1,722 1,379 - 1,871 422 - 1,069 398 - 712 288-751 1.89-1,669 75-2,006 403-1,330 502-2,471 375-2,431 449-2,425 679-1,872 766-1,780 564-2,198 304-2,189 321-1,896 59-1,132 Total taxa 45 -50 19 - 33 18 - 32 19 - 37 32 - 35 26 - 28 27 - 30 29 - 34 22 - 27 23 - 31 26-33 13-33 ' 12-38 20-36 14-33 24-35 22-37 20-40 23-33 18-32 16-41 17-32 15-32 EBI 1.77 - 1.81 1.93 - 2.15 1.77 - 2.20 1.77 - 1.94 1.93 - 2.42 1.88 - 2.04 2.05 - 2.19 1.80 - 2.05 1.95 - 2.03 1.75 - 2.47 2.00-2.08 1.96-2.19 1.90-2.26 1.78-2.33 1.82-2.25 1.77-2.07 1.79-2.11 1.74-2.17 1.79-2.08 1.84-2.11 1.34-2.18 1.64-2.36 1.48-1.98 Averages All creeks (up) Total abundance 2,328 1,079 1,729 1,932 1,243 1,011 1,561 1,710 1,398 675 624 940 1,795 1,116 1,341 1,411 1,620 1,473 1,465 1,392 1,270 1,049 892 Total taxa 39 22 29 29 32 25 38 34 26 23 22 19 29 26 24 28 27 29 30 28 31 23 26 EBIa 1.79 1.95 1.80 1.93 1.88 1.75 1.96 2.00 1.98 2.27 1.97 1.87 1.98 2.03 1.91 1.77 1.83 1.87 1.80 1.83 1.64 1.88 1.72 All creeks (down) Total abundance 2,966 999 900 1,543 831 780 1,368 1,625 746 555 520 954 1,422 899 1,273 1,562 1,603 1,344 1,351 1,269 1,098 1,027 558 Total taxa 47 27 27 29 34 27 29 32 25 27 30 25 30 28 28 31 31 29 29 27 30 23 23 EBIa 1.79 2.03 1.95 1.87 2.18 1.96 2.12 1.93 1.99 2.11 2.04 2.05 2.05 2.07 1.99 1.92 1.97 1.97 1.94 1.98 1.86 1.94 1.86 III-G-87 Table III-G4. Dominant benthic macroinvertebrate species for ponar grab samples collected in monitored creeks during May of Mod Alt L sample years 1998-2005 and 2007-2021. Pre -Mod Alt L: Broomfield Swamp Creek (2019-2021), Jacks Creek (1998-2005; 2011-2014), Jacobs Creek (2011-2013), Drinkwater Creek (2011-2012), Tooley Creek (1998-2001; 2010-2011), Huddles Cut (1998-2001; 2007-2009), Porter Creek (2011-2015), and DCUT11 (2013-2017). Post -Mod Alt L: Jacks Creek (2015-2021), Jacobs Creek (2014-2021), Drinkwater Creek (2013-2021), Tooley Creek (2012-2021), Huddles Cut (2010- 2021), Porter Creek (2016-2021), and DCUT11 (2018-2021). Control Creeks: SCUT1 (2019-2021), PA2, Long, Little, and Duck creeks (2011-2021), Muddy Creek (1998-2005 and 2007-2021), and DCUT19 (2013-2021). A - indicates the creek was not monitored that year. Benthic collection occurred in June for 2010. In each ponar grab sample, count of individuals per species stopped at 100. BROOMFIELD CREEK UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Naididae sp. w/o hair Limnodrilus hoffmeisteri Chaoborus albatus Glossiphoniidae sp. 163 56 33 33 47.0 16.1 9-5 9.5 Naididae sp. w/o hair Limnodrilus hoffmeisteri Gammarus tigrinus Streblospio benedicti 44 24 14 3 50.6 27.6 16.1 3.4 Naididae sp. w/o hair Chironomus decorus Procladius sp. Tanypus neopunctipennis 140 29 29 29 47.1 9.8 9.8 9.8 BROOMFIELD CREEK DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Naididae sp. w/o hair Limnodrilus hoffmeisteri Chironomus decorus Naididae sp. w/ hair 217 78 21 10 61.0 21.9 5.9 2.8 Gammarus tigrinus Streblospio benedicti Limnodrilus hoffmeisteri Mytilopsis leucophaeata 105 8 7 6 72.9 5.6 4.9 4.2 Naididae sp. w/o hair Limnodrilus hoffmeisteri Dicrotendipes nervosus Amphicteis floridus 13 8 3 2 35.1 21.6 8.1 5.4 SCUT1 (CONTROL) UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Naididae sp. w/o hair Tanypus neopunctipennis Coelotanypus sp. Limnodrilus hoffmeisteri 283 48 37 34 55.6 9.4 7.3 6.7 Gammarus tigrinus Naididae sp. w/o hair Cyprideis littoralis Limnodrilus hoffmeisteri 222 69 31 5 66.5 20.7 9.3 1.5 Naididae sp. w/o hair Chironomus decorus Tanypus neopunctipennis Procladius sp. 113 95 57 27 29.0 24.4 14.7 6.9 r - J SCUT1 (CONTROL) DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Procladius sp. Candonidae sp. Chironomus decorus Cyprideis littoralis 62 44 38 22 28.3 20.1 17.4 10.0 Gammarus tigrinus Amphicteis floridus Cyprideis littoralis Streblospio benedicti 34 18 11 11 40.0 19.6 12.0 12.0 Gammarus tigrinus Amphicteis floridus Tubificoides heterochaetus Cryptochironomus sp. 156 102 4 4 56.3 36.8 1.4 1.4 III-G-88 Table III-G4 (continued). JACKS CREEK UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Gammarus tigrinus 61 28.4 Gammarus tigrinus 418 81.2 Tanypus neopunctipennis 406 36.1 Gammarus tigrinus 424 31.1 Macoma balthica 163 54.2 Chironomus decorus 474 77.1 Tanytarsus Iimneticus 167 30.4 Chironomus decorus 183 39.5 Amphicteis floridus 58 27.0 Nematoda sp. 50 9.7 Amphicteis floridus 324 28.9 Dicrotendipes nervosus 418 30.7 Littoridinops tenuipes 90 30.0 Amphicteis floridus 41 6.7 Cyprideis Iittoralis 117 21.3 Tanytarsus sp. 1 84 18.1 Littoridinops tenuipes 17 7.9 Amphicteis floridus 31 6.0 Gammarus tigrinus 101 9.0 Amphicteis floridus 213 15.6 Bezzia/Palpomyia complex 12 4.0 Gammarus tigrinus 38 6.2 Littoridinops tenuipes 89 16.2 Tanypus neopunctipennis 38 8.2 Procladius sp. 16 7.4 Littoridinops tenuipes 5 1.0 Chironomus decorus 100 8.9 Chironomus decorus 109 8.0 Cyprideis Iittoralis 8 2.7 Littoridinops tenuipes 34 5.5 Gammarus tigrinus 27 4.9 Gammarus tigrinus 37 8.0 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - not monitored - - Chironomus decorus 133 24.2 Candonidae sp. 167 65.5 Chironomus decorus 150 39.2 Chironomus decorus 286 30.4 - - - - - - - - - - - - Macoma balthica 88 16.0 Chironomus decorus 80 31.4 Candonidae sp. 136 35.5 Amphicteis floridus 174 18.5 - - - - - - - - - - - - Apocorophium sp. 80 14.6 Naididae sp. w/o hair 4 1.6 Littoridinops tenuipes 29 7.6 Gammarus tigrinus 122 13.0 - - - - - - - - - - - - Gammarus tigrinus 72 13.1 Tanytarsus sp. 2 0.8 Apedilum sp. 26 6.8 Candonidae sp. 104 11.1 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Gammarus tigrinus 97 27.5 Marenzelleria viridis 27 30.0 Gammarus tigrinus 162 29.5 Gammarus tigrinus 330 51.9 Chironomus decorus 44 35.8 Chironomus decorus 35 24.6 Chironomus decorus 77 41.0 Amphicteis floridus 84 23.8 Gammarus tigrinus 26 28.9 Littoridinops sp. 71 12.9 Chironomus decorus 71 11.2 Amphicteis floridus 15 12.2 Candonidae sp. 34 23.9 Tanypus neopunctipennis 50 26.6 Littoridinops sp. 50 14.2 Apocorophium sp. 8 8.9 Tanytarsus sp. 66 12.0 Apocorophium sp. 44 6.9 Tanypus neopunctipennis 14 11.4 Gammarus tigrinus 18 12.7 Laeonereis culveri 29 15.4 Apocorophium sp. 21 5.9 Amphicteis floridus 7 7.8 Amphicteis floridus 54 9.8 Parachironomus sp. 44 6.9 Gammarus tigrinus 10 8.1 Littoridinops tenuipes 18 12.7 Amphicteis floridus 9 4.8 X M. X JACKS CREEK DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus decorus 49 22.2 Amphicteis floridus 103 37.6 Chironomus decorus 192 61.1 Gammarus tigrinus 106 26.6 Macoma balthica 443 35.6 Tubificoides sp. 500 30.5 Purbifidoides sp. 239 33.2 Cyprideis Iittoralis 398 39.1 Tubificoides sp. 42 19.0 Leptocheirus plumulosus 37 13.5 Procladius sp. 60 19.1 Macoma balthica 81 20.4 Mediomastus ambiseta 399 32.0 Mediomastus ambiseta 480 29.3 Marenzelleria viridis 118 16.4 Chironomus decorus 349 34.3 Cyprideis Iittoralis 36 16.3 Marenzelleria viridis 25 9.1 Tubificoides sp. 30 9.6 Mediomastus ambiseta 76 19.1 Tubificoides sp. 214 17.2 Streblospio benedicti 355 21.7 Streblospio benedicti 99 13.8 Streblospio benedicti 108 10.6 Amphicteis floridus 26 11.8 Gammarus tigrinus 23 8.4 Amphicteis floridus 11 3.5 Marenzelleria viridis 51 12.8 Littoridinops tenuipes 43 3.5 Mediomastus sp. 231 14.1 Littoridinops tenuipes 57 7.9 Macoma balthica 51 5.0 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - not monitored - - Macoma balthica 280 25.7 Chironomus decorus 145 66.2 Chironomus decorus 268 62.8 Chironomus decorus 45 24.6 - - - - - - - - - - - - Chironomus decorus 234 21.5 Streblospio benedicti 24 11.0 Gammarus mucronatus 40 9.4 Streblospio benedicti 21 11.5 - - - - - - - - - - - - Streblospio benedicti 102 9.4 Candonidae sp. 18 8.2 Gammarus tigrinus 25 5.9 Amphicteis floridus 20 10.9 - - - - - - - - - - - - Gammarus tigrinus 94 8.6 Macoma tenta 8 3.7 Macoma tenta 24 5.6 Macoma tenta 20 10.9 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus sp. 335 40.3 Marenzelleria viridis 181 57.5 Marenzelleria viridis 135 36.3 Gammarus tigrinus 142 32.6 Chironomus decorus 148 77.1 Gammarus tigrinus 223 47.1 Chironomus decorus 467 82.8 Streblospio benedicti 162 19.5 Apocorophium sp. 47 14.9 Rangia cuneata 79 21.2 Apocorophium sp. 138 31.7 Amphicteis floridus 17 8.9 Apocorophium sp. 96 20.3 Littoridinops sp. 21 3.7 Gammarus tigrinus 68 8.2 Apocorophium Iacustre 29 9.2 Gammarus tigrinus 43 11.6 Mediomastus ambiseta 32 7.3 Cyprideis Iittoralis 6 3.1 Littoridinops tenuipes 29 6.1 Streblospio benedicti 19 3.4 Naididae sp. w/o hair 59 7.1 Gammarus tigrinus 13 4.1 Cyprideis Iittoralis 43 11.6 Apocorophium Iouisianum 24 5.5 Gammarus tigrinus 5 2.6 Streblospio benedicti 26 5.5 Amphicteis floridus 16 2.8 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - Y 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % W W ct not monitored - - not monitored - - not monitored - - not monitored - - Aulodrilus sp. 110 27.8 Chironomus decorus 244 36.0 Apedilum sp. 436 34.1 Chironomus decorus 222 39.7 Q (,) W 0 - - - - - - - - - - - - Macoma balthica 109 27.6 Littoridinops tenuipes 143 21.1 Chironomus decorus 298 23.3 Candonidae sp. 126 22.5 Cr) H - - - - - - - - - - - - Amphicteis floridus 37 9.4 Apedilum sp. 119 17.6 Candonidae sp. 208 16.3 Parachironomus sp. 55 10.0 Of/) fi - - - - - - - - - - - - Apocorophium sp. 31 7.8 Candonidae sp. 52 7.7 Littoridinops tenuipes 67 5.2 Littoridinops tenuipes 49 9.8 2015 2016 2017 2018 2019 2020 2021 U m Q Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Gammarus tigrinus 269 23.2 Littoridinops sp. 13 22.0 Littoridinops sp. 322 32.5 Apocorophium sp. 121 24.5 Chironomus decorus 147 43.1 Apocorophium sp. 33 19.4 Chironomus decorus 104 59.8 Apocorophium sp. 255 22.0 Apocorophium sp. 9 15.3 Amphicteis floridus 194 19.6 Chironomus decorus 117 23.7 Amphicteis floridus 41 12.0 Chironomus decorus 31 18.2 Tanypus neopunctipennis 22 12.6 Amphicteis floridus 204 17.6 Marenzelleria viridis 9 15.3 Apocorophium sp. 140 14.1 Macoma balthica 69 14.0 Tanypus neopunctipennis 36 10.6 Gammarus tigrinus 24 14.1 Laeonereis culveri 19 10.9 Littoridinops sp. 116 10.0 Cyprideis Iittoralis 5 8.5 Gammarus tigrinus 78 7.9 Gammarus tigrinus 57 11.6 Tanytarsus sp. 35 10.3 Corixidae sp. 14 8.2 Gammarus tigrinus 6 3.4 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - Y 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % w 11 W not monitored - - not monitored - - not monitored - - not monitored - - Chironomus decorus 184 29.3 Apedilum sp. 240 39.5 Chironomus decorus 319 54.2 Chironomus decorus 129 41.7 () - - - - - - - - - - - - Macoma balthica 137 21.8 Mediomastus ambiseta 144 23.7 Apocorophium sp. 85 14.4 Gammarus mucronatus 71 23.0 Cr) Cr) - - - - - - - - - - - - Mediomastus ambiseta 113 18.0 Chironomus decorus 124 20.9 Streblospio benedicti 32 5.4 Mediomastus ambiseta 36 11.7 CO Z - - - - - - Apocorophium sp. 60 9.6 Parachironomus sp. 54 8.9 Apedilum sp. 27 4.6 Macoma tenta 26 8.4 2015 2016 2017 2018 2019 2020 2021 ct p Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus sp. 443 65.2 Marenzelleria viridis 104 48.8 Marenzelleria viridis 293 33.4 Mediomastus ambiseta 226 31.9 Chironomus decorus 491 79.7 Apocorophium sp. 162 43.8 Chironomus decorus 430 89.6 Gammarus tigrinus 58 8.5 Mediomastus ambiseta 37 17.4 Mediomastus ambiseta 238 27.1 Macoma tenta 130 18.4 Streblospio benedicti 37 6.0 Gammarus tigrinus 51 13.8 Streblospio benedicti 17 3.5 Streblospio benedicti 33 4.9 Apocorophium sp. 15 7.0 Gammarus tigrinus 74 8.4 Chironomus decorus 122 17.2 Littoridinops sp. 35 5.7 Gammarus mucronatus 33 8.9 Tanypus neopunctipennis 8 1.7 Eteone heteropoda 20 2.9 Eteone heteropoda 13 6.1 Rangia cuneata 61 7.0 Gammarus tigrinus 48 6.8 Americamysis almyra 14 2.3 Eteone heteropoda 32 8.6 Americamysis almyra 8 1.7 III-G-89 Table III-G4 (continued). 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total Dominant Species Total 0/0 Dominant Species Total 0/0 Dominant Species Total Dominant Species Total Dominant Species Total 0/0 Dominant Species Total Dominant Species Total not monitored not monitored not monitored not monitored not monitored not monitored not monitored not monitored 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total 0/0 Dominant Species Total Dominant Species Total 0/0 Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total not monitored not monitored not monitored not monitored Apocorophium sp. Chironomus decorus Littondinops tenuipes Apedilum sp. 205 194 69 62 26.1 24.7 8.8 7.9 Candonidae sp. Littondinops tenuipes Apedilum sp. Chironomus decorus 385 68 63 63 65.7 11.6 10.8 Apedilum sp. Chironomus decorus Candonidae sp. 10.8 Goeldichironomus devineyae 253 38 26 19 61.6 9.2 6.3 4.6 Littondinops tenuipes Chironomus decorus Goeldichironomus devineyae Apocorophium sp. 141 71 23 19 ok 46.2 23.3 7.5 6.2 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species (Total Ok Chironomus sp. Amphicteis flondus Tanypus neopunctipennis Littondinops sp. 384 68.2 30 5.3 29 5.2 27 4.8 Chironomus decorus Streblospio benedicti Gammarus tignnus Marenzellena viridis 376 59 38 32 67.9 10.7 6.9 5.8 Littondinops sp. Amphicteis flondus Corixidae sp. Apedilum sp. 167 141 29 14 42.3 35.7 7.3 3.5 Chironomus decorus Gammarus tignnus Apocorophium sp. Candonidae sp. 138 80 62 54 27.2 15.8 12.2 10.7 Chironomus decorus Tanytarsus sp. Littondinops sp. Tanypus neopunctipennis 363 58 57 30 61.6 9.8 9.7 5.1 Apocorophium sp. Littondinops tenuipes Amphicteis flondus Apedilum sp. 76 64 63 43 20.9 Chironomus decorus 17.6 Tanypus neopunctipennis 17.4 Laeonereis culvert 11.8 Littondinops sp. 244 36 28 18 65.2 9.6 7.5 4.8 1998 1999 2000 2001 1 2002 2003 2004 2005 Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total not monitored not monitored not monitored not monitored not monitored not monitored not monitored not monitored 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total not monitored not monitored not monitored not monitored Chironomus decorus Apocorophium sp. Aulodnlus sp. Apedilum sp. 101 79 61 52 23.0 18.0 13.9 11.8 Chironomus decorus Apedilum sp. Candonidae sp. Littondinops tenuipes 488 123 111 31 62.9 Apedilum sp. 15.9 Chironomus decorus 14.3 Gammarus mucronatus 4.0 Littondinops tenuipes 204 81 54 20 45.4 18.0 12.0 4.5 Naididae sp. w/o hair Gammarus mucronatus Chironomus decorus Littondinops tenuipes 64 36 30 22 36.8 20.7 17.2 12.6 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total Dominant Species Total Ok Dominant Species Total Dominant Species ITotall Dominant Species Total Dominant Species Total Dominant Species Total Gammarus tignnus Tanytarsus sp. Littondinops sp. Tanypus neopunctipennis 1998 159 152 106 83 • 20.3 19.4 13.5 10.6 Marenzellena viridis Apocorophium sp. Streblospio benedicti Corixidae sp. 1999 361 164 99 54 37.2 16.9 10.2 5.6 Marenzellena viridis Amphicteis flondus Littondinops sp. Laeonereis culvert 2000 470 325 173 39 38.6 26.7 14.2 3.2 Gammarus tignnus Chironomus decorus Streblospio benedicti Apocorophium sp. 2001 20.6 19.0 12.5 10.3 Chironomus decorus Littondinops sp. Amphicteis flondus Tanypus neopunctipennis 2002 402 87 44 43 60.4 13.1 6.6 6.5 Littondinops tenuipes Apocorophium sp. Gammarus tignnus Amphicteis flondus 2003 40 39 21 10 29.2 28.5 15.3 7.3 Laeonereis culvert Amphicteis flondus Chironomus decorus Littondinops sp. 2004 187 24.5 180 23.6 138 18.1 62 8.1 2005 Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total 0/0 Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total not monitored not monitored not monitored not monitored not monitored not monitored not monitored not monitored 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Ok Dominant Species Total Dominant Species Total Dominant Species Total not monitored not monitored not monitored not monitored Tanytarsus sp. Aulodnlus sp. Littondinops tenuipes Apocorophium sp. 269 154 148 146 21.8 12.5 12.0 11.8 Chironomus decorus Littondinops tenuipes Candonidae sp. Amphicteis flondus 478 244 126 92 37.7 Chironomus decorus 19.3 Littondinops tenuipes 9.9 Amphicteis flondus 7.3 Goeldichironomus devineyae 452 169 63 32 54.7 20.4 7.6 3.9 Chironomus decorus Amphicteis flondus Naididae sp. w/o hair Gammarus tignnus 358 96 69 63 36.8 9.8 7.1 6.5 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Littondinops sp. Chironomus sp. Tanytarsus sp. Amphicteis flondus 67 25.3 63 23.8 30 11.3 27 10.2 Amphicteis flondus Gammarus tignnus Marenzellena viridis Apocorophium sp. 118 32.5 86 23.7 64 17.6 40 11.0 Amphicteis flondus Gammarus tignnus Littondinops sp. Apocorophium sp. 309 278 174 35 33.2 29.9 18.7 3.8 Chironomus decorus Gammarus tignnus Apocorophium sp. Candonidae sp. 359 46.8 145 18.9 83 10.8 52 6.8 Chironomus stigmaterus Candonidae sp. Amphicteis flondus Tanytarsus sp. 149 92 87 81 28.1 17.4 16.4 15.3 Gammarus tignnus Apocorophium sp. Chironomus decorus Amphicteis flondus 64.0 46.0 36.0 22.0 26.4 Gammarus tignnus 19 Chironomus decorus 14.9 Dicrotendipes nervosus 9.1 Amphicteis flondus 164 47.8 75 21.9 24 7.0 21 6.1 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total Dominant Species Total not monitored not monitored not monitored not monitored not monitored not monitored not monitored not monitored 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total Dominant Species Total 0/0 Dominant Species Total Dominant Species Total 0/0 Dominant Species Total Dominant Species Total Dominant Species Total Ok Dominant Species Total not monitored not monitored not monitored not monitored Chironomus decorus Macoma balthica Mediomastus ambiseta Parachironomus sp. 245 194 179 88 22.5 17.8 16.4 8.1 Chironomus decorus Apedilum sp. Parachironomus sp. Littondinops tenuipes 415 256 52 28 51.3 Chironomus decorus 31.6 Gammarus mucronatus 6.4 Apocorophium louisianum 3.5 Apocorophium sp. 231 140 136 134 22.4 13.6 13.2 13.0 Chironomus decorus Gammarus mucronatus Mediomastus ambiseta Streblospio benedicti 415 270 113 98 37.3 24.3 10.2 8.8 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total Dominant Species Total % Dominant Species Total Ok Dominant Species Total % Dominant Species Total % Dominant Species Total 0/0 Chironomus sp. Macoma tenta Marenzelleria viridis Streblospio benedicti 38 29 25 18 23.6 18.0 15.5 11.2 Marenzelleria viridis Littoridinops sp. Streblospio benedicti Mediomastus ambiseta 223 31 28 21 63.0 8.8 7.9 5.9 Marenzelleria viridis Littoridinops sp. Mediomastus ambiseta Corixidae sp. 180 108 52 42 37.3 22.4 10.8 8.7 Mediomastus ambiseta Chironomus decorus Gammarus tigrinus Streblospio benedicti 266 142 127 123 23.4 12.5 11.2 10.8 Chironomus decorus Streblospio benedicti Amphicteis floridus Chironomus stigmaterus 486 110 21 15 72.2 16.3 3.1 2.2 Apocorophium sp. Streblospio benedicti Gammarus tigrinus Mediomastus ambiseta 88 71 48 36 22.6 Chironomus decorus 18.2 Streblospio benedicti 9.2 Mediomastus ambiseta 445 30 10 6 87.6 5.9 2.0 1.2 Table III-G4 (continued). J O C' I- Z 0 Q `-' W Y W I- W f/) C' 0- U D W J ~ � J 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - - - - not monitored - - - - - Littoridinops tenuipes Amphicteis floridus Gammarus tigrinus Tanytarsus sp. 410 88 36 26 67.2 14.4 5.9 4.3 Tanytarsus sp. Candonidae sp. Littoridinops tenuipes Chironomus decorus 416 408 254 212 29.8 29.3 18.2 15.2 Gammarus tigrinus Tanytarsus sp. Littoridinops tenuipes Amphicteis floridus 429 366 199 188 23.0 19.6 10.7 10.1 Chironomus decorus Gammarus tigrinus Amphicteis floridus Littoridinops tenuipes 100 96 58 36 27.3 26.2 15.8 9.8 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Gammarus tigrinus Naididae sp. w/o hair Amphicteis floridus Littoridinops sp. 284 78 60 30 54.4 14.9 11.5 5.7 Gammarus tigrinus Amphicteis floridus Cyprideis littoral's Chironomus decorus 230 191 90 26 36.8 30.6 14.4 4.2 Chironomus decorus Naididae sp. w/o hair Gammarus tigrinus Candonidae sp. 399 179 153 99 34.5 15.5 13.2 8.5 Amphicteis floridus Gammarus tigrinus Chironomus decorus Naididae sp. w/o hair 464 306 242 62 35.8 23.6 18.7 4.8 Gammarus tigrinus Naididae sp. w/o hair Kiefferulus sp. Chironomus decorus 255 147 96 90 34.8 20.1 13.1 12.3 Gammarus tigrinus Cyprideis littoral's Amphicteis floridus Bezzia/Palpomyia complex 45 10 9 3 65.2 14.5 13.0 4.3 Chironomus decorus Amphicteis floridus Limnodrilus hoffmeisteri Procladius sp. 252 202 28 12 47.2 37.8 5.2 2.2 J O C' I- Z Q 0 W Y F- LU � U O W CI J I- I- J 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - - - - not monitored - - - - - not monitored - - - - - not monitored - - - - - Marenzelleria viridis Macoma balthica Streblospio benedicti Aulodrilus sp. 267 95 58 53 42.4 15.1 9.2 8.4 Mediomastus ambiseta Chironomus decorus Macoma tents Macoma balthica 306 94 56 42 57.4 17.6 10.5 7.9 Chironomus decorus Apocorophium louisianum Gammarus mucronatus Amphicteis floridus 500 262 255 214 24.8 13.0 12.6 10.6 Chironomus decorus Streblospio benedicti Amphicteis floridus Mediomastus ambiseta 428 116 98 84 48.1 13.0 11.0 9.4 2015 2016 2017 2018 2019 2020 2021 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Apocorophium sp. Streblospio benedicti Chironomus sp. Amphicteis floridus 396 396 302 230 20.2 20.2 15.4 11.8 Marenzelleria viridis Chironomus decorus Apocorophium sp. Mediomastus ambiseta 158 109 52 38 33.1 22.8 10.9 8.0 Marenzelleria viridis Apocorophium lacustre Apocorophium sp. Amphicteis floridus 328 166 162 154 26.9 13.6 13.3 12.6 Streblospio benedicti Mediomastus ambiseta Gammarus tigrinus Macoma balthica 361 188 150 55 36.5 19.0 15.2 5.6 Chironomus decorus Streblospio benedicti Littoridinops sp. Gammarus tigrinus 485 62 34 5 80.7 10.3 5.7 0.8 Apocorophium sp. Streblospio benedicti Mediomastus ambiseta Parachironomus sp. 112 41 39 28 31.6 11.6 11.0 7.9 Chironomus decorus Tanypus neopunctipennis Streblospio benedicti Procladius sp. 2004 500 14 13 3 92.8 2.6 2.4 0.6 L -IDominant O C' ZDominant O Q U LLI Y W w f/) 0 CL 0Dominant Z O 1998 1999 2000 2001 2002 2003 Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - - - - not monitored - - - - - not monitored - - - - - not monitored - - - - - Apedilum sp. Macoma balthica Chironomus decorus Aulodrilus sp. 170 101 73 35 32.9 19.5 14.1 6.8 Chironomus decorus Macoma balthica Macoma tenta Naididae sp. w/o hair 224 37 31 24 59.6 9.8 8.2 6.4 Chironomus decorus Gammarus mucronatus Mediomastus ambiseta Amphicteis floridus 500 130 110 60 53.1 13.8 11.7 6.4 Chironomus decorus Gammarus mucronatus Eteone heteropoda Apocorophium sp. 450 54 48 42 62.6 7.5 6.7 5.8 2015 2016 2017 2018 2019 2020 2021 Species Total / Dominant Species Total / Dominant Species Total / Dominant Species Total / Dominant Species Total / Dominant Species Total / Dominant Species Total Chironomus sp. Apocorophium sp. Gammarus tigrinus Streblospio benedicti 320 66 49 48 50.6 10.4 7.7 7.6 Marenzelleria viridis Chironomus decorus Gammarus tigrinus Streblospio benedicti 31 22 12 4 36.0 25.6 14.0 4.7 Gammarus tigrinus Marenzelleria viridis Littoridinops sp. Chironomus decorus 209 134 115 109 25.4 16.3 14.0 13.3 Gammarus tigrinus Streblospio benedicti Macoma balthica Gammarus mucronatus 130 74 67 66 23.2 13.2 11.9 11.8 Chironomus decorus Littoridinops sp. Gammarus tigrinus Streblospio benedicti 179 16 15 12 73.1 6.5 6.1 4.9 Apocorophium sp. Gammarus tigrinus Gammarus mucronatus Eteone heteropoda 144 123 32 27 35.9 30.7 8.0 6.7 Chironomus decorus Tanypus neopunctipennis Amphicteis floridus Gammarus tigrinus 457 15 12 10 89.6 2.9 2.3 2.0 LONG CREEK (CONTROL) DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - Macoma balthica Marenzelleria viridis Mediomastus ambiseta Eteone heteropoda 124 58 10 8 57.4 26.9 4.6 3.7 Mediomastus ambiseta Macoma tenta Streblospio benedicti Macoma balthica 500 77 76 52 65.0 10.0 9.9 6.8 Mediomastus ambiseta Chironomus decorus Scoloplos robustus Streblospio benedicti 125 105 52 45 34.4 29.0 14.3 12.4 Chironomus decorus Macoma tenta Macoma balthica Mediomastus ambiseta 148 78 24 5 54.2 28.6 8.8 1.8 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Chironomus sp. Streblospio benedicti Mediomastus ambiseta Macoma tenta 406 295 80 65 43.1 31.3 8.5 6.9 Littoridinops sp. Chironomus decorus Macoma tenta Streblospio benedicti 43 42 24 15 24.4 23.9 13.6 8.5 Marenzelleria viridis Chironomus decorus Littoridinops sp. Macoma tenta 101 62 32 25 34.1 20.9 10.8 8.4 Streblospio benedicti Balanus improvisus Macoma tenta Gammarus mucronatus 49 46 39 31 14.0 13.1 11.1 8.8 Chironomus decorus Littoridinops sp. Streblospio benedicti Gammarus tigrinus 421 31 31 11 79.1 5.8 5.8 2.1 Streblospio benedicti Mediomastus ambiseta Macoma tenta Gammarus tigrinus 22 21 13 5 31.4 30.0 18.6 7.1 Chironomus decorus Streblospio benedicti Tanypus neopunctipennis Littoridinops sp. 442 47 14 2 86.5 9.2 2.7 0.4 III-G-91 Table III-G4 (continued). TOOLEY CREEK UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Gammarus tigrinus 118 24.8 Gammarus tigrinus 206 64.4 Chironomus decorus 418 91.1 Gammarus tigrinus 382 40.8 not monitored - - not monitored - - not monitored - - not monitored - - Amphicteis floridus 90 18.9 Nematoda sp. 33 10.3 Littoridinops tenuipes 11 2.4 Macoma balthica 226 24.1 - - - - - - - - - - - - Littoridinops tenuipes 80 16.8 Amphicteis floridus 32 10.0 Ostracoda sp. 7 1.5 Chironomus decorus 104 11.1 - - - - - - - - - - - - Cyprideis littoralis 78 16.4 Leptocheirus plumulosus 13 4.1 Tanypus neopunctipennis 6 1.3 Amphicteis floridus 87 9.3 - - - - - - - - - - - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - Chironomus decorus 48 34.8 Macoma balthica 116 30.3 Chironomus decorus 96 61.9 Chironomus decorus 439 46.7 Naididae sp. w/o hair 56 24.1 - - - - - - - - - Macoma tenta 24 17.4 Chironomus decorus 61 15.9 Naididae sp. w/o hair 15 9.7 Apocorophium sp. 107 11.4 Macoma balthica 41 17.7 - - - - - - - - - Naididae sp. w/o hair 22 15.9 Apocorophium sp. 41 10.7 Streblospio benedicti 14 9.0 Amphicteis floridus 105 11.2 Chironomus decorus 38 16.4 - - - - - - - - - Tubificoides sp. 11 8.0 Gammarus tigrinus 38 9.9 Candonidae sp. 10 6.5 Gammarus mucronatus 102 10.8 Gammarus mucronatus 13 5.6 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus sp. 77 33.2 Marenzelleria viridis 26 40.0 Littoridinops sp. 55 21.8 Macoma balthica 51 25.8 Chironomus decorus 136 37.8 Littoridinops tenuipes 9 18.0 Chironomus decorus 228 72.2 Apocorophium sp. 54 23.3 Bezzia/Palpomyia complex 6 9.2 Marenzelleria viridis 48 19.0 Chironomus decorus 49 24.7 Amphicteis floridus 42 11.7 Apocorophium sp. 6 12.0 Americamysis almyra 22 7.0 Tanytarsus sp. 22 9.5 Streblospio benedicti 6 9.2 Gammarus tigrinus 41 16.3 Gammarus tigrinus 19 9.6 Cyprideis littoralis 34 9.4 Mediomastus ambiseta 6 12.0 Streblospio benedicti 14 4.4 Littoridinops sp. 13 5.6 Eteone heteropoda 5 7.7 Chironomus decorus 27 10.7 Apocorophium sp. 18 9.1 Apocorophium sp. 33 9.2 Streblospio benedicti 6 12 Tanypus neopunctipennis 11 3.5 TOOLEY CREEK DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus decorus 366 39.8 Gammarus tigrinus 70 25.9 Chironomus decorus 423 56.9 Macoma balthica 444 40.8 not monitored - - not monitored - - not monitored - - not monitored - - Tubificoides sp. 289 31.4 Apocorophium lacustre 42 15.6 Tubificoides sp. 158 21.3 Mediomastus ambiseta 368 33.8 - - - - - - - - - - - - Gammarus tigrinus 81 8.8 Amphicteis floridus 36 13.3 Amphicteis floridus 68 9.2 Cyprideis littoralis 47 4.3 - - - - - - - - - - - - Apocorophium lacustre 57 6.2 Leptocheirus plumulosus 28 10.4 Rangia cuneata 23 3.1 Macoma tenta 34 3.1 - - - - - - - - - - - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - Macoma tenta 151 29.5 Mediomastus ambiseta 159 37.9 Mediomastus ambiseta 500 35.3 Chironomus decorus 457 52.1 Chironomus decorus 391 48.2 - - - - - - - - - Chironomus decorus 103 20.1 Chironomus decorus 90 21.5 Chironomus decorus 353 24.9 Mediomastus ambiseta 190 21.7 Macoma tenta 268 33.0 - - - - - - - - - Mediomastus sp. 80 15.6 Parachironomus sp. 40 9.5 Streblospio benedicti 350 24.7 Streblospio benedicti 119 13.6 Mediomastus ambiseta 62 7.6 - - - - - - - - - Cyprideis littoralis 33 6.4 Apedilum sp. 39 9.3 Tubificoides sp. 92 6.5 Apocorophium sp. 20 2.3 Macoma balthica 48 5.9 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus sp. 500 50.3 Littoridinops sp. 89 31.2 Rangia cuneata 133 19.9 Mediomastus ambiseta 250 23.7 Chironomus decorus 264 74.4 Mediomastus ambiseta 187 68.8 Chironomus decorus 432 84.5 Mediomastus ambiseta 139 14.0 Macoma tenta 78 27.4 Marenzelleria viridis 130 19.4 Balanus improvisus 214 20.3 Streblospio benedicti 48 13.5 Streblospio benedicti 21 7.7 Streblospio benedicti 40 7.8 Tubificoides heterochaetus 114 11.5 Marenzelleria viridis 27 9.5 Mediomastus ambiseta 126 18.8 Macoma tenta 154 14.6 Mediomastus ambiseta 11 3.1 Macoma tenta 18 6.6 Macoma tenta 15 2.9 Streblospio benedicti 113 11.4 Mediomastus ambiseta 22 7.7 Tubificoides heterochaetus 78 11.7 Macoma balthica 141 13.4 Tubificoides heterochaetus 11 3.1 Chironomus decorus 17 6.3 Mediomastus ambiseta 7 1.4 MUDDY CREEK (CONTROL) UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Gammarus tigrinus 410 34.4 Amphicteis floridus 59 39.6 Chironomus decorus 446 82.3 Ostracoda sp. 17 37.0 Chironomus decorus 41 54.7 Chironomus decorus 41 44.1 Ostracoda sp. 13 28.3 Apocorophium lacustre 174 26.8 Amphicteis floridus 301 25.3 Gammarus tigrinus 41 27.5 Procladius sp. 52 9.6 Macoma balthica 14 30.4 Apedilum sp. 11 14.7 Macoma balthica 31 33.3 Rangia cuneata 13 28.3 Gammarus tigrinus 164 25.2 Apocorophium lacustre 265 22.3 Nematoda sp. 13 8.7 Amphicteis floridus 24 4.4 Cyprideis littoralis 7 15.2 Littoridinops tenuipes 7 9.3 Goeldichironomus devineyae 7 7.5 Littoridinops tenuipes 7 15.2 Chironomus decorus 131 20.2 Edotia triloba 55 4.6 Littoridinops tenuipes 9 6.0 Ostracoda sp. 6 1.1 Chironomus decorus 5 10.9 Streblospio benedicti 6 8.0 Littoridinops tenuipes 6 6.5 Chironomus decorus 4 8.7 Macoma balthica 49 7.5 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus decorus 175 60.1 Streblospio benedicti 12 32.4 Chironomus decorus 59 30.9 Ostracoda sp. 48 71.6 Chironomus decorus 145 62.0 Macoma balthica 116 57.1 Chironomus decorus 113 70.6 Candonidae sp. 31 63.3 Streblospio benedicti 28 9.6 Ostracoda sp. 6 16.2 Parachironomus directus 22 11.5 Chironomus decorus 10 14.9 Aulodrilus sp. 54 23.1 Mediomastus ambiseta 40 19.7 Candonidae sp. 17 10.6 Chironomus decorus 9 18.4 Cyprideis littoralis 26 8.9 Chironomus decorus 5 13.5 Ostracoda sp. 20 10.4 Littoridinops tenuipes 3 4.5 Streblospio benedicti 14 6.0 Candonidae sp. 23 11.3 Streblospio benedicti 9 5.6 Gammarus mucronatus 2 4.1 Tanypus neopunctipennis 18 6.2 Macoma balthica 5 13.5 Macoma balthica 19 9.9 Apocorophium sp. 2 3.0 Amphicteis floridus 5 2.1 Macoma tenta 14 6.9 Amphicteis floridus 7 4.4 Parachironomus sp. 2 4.1 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus sp. 149 63.4 Chironomus decorus 100 33.2 Apocorophium sp. 100 35.2 Chironomus decorus 85 28.1 Candonidae sp. 125 56.8 Candonidae sp. 97 48.7 Chironomus decorus 219 85.5 Streblospio benedicti 35 14.9 Streblospio benedicti 72 23.9 Gammarus tigrinus 42 14.8 Candonidae sp. 80 26.5 Chironomus decorus 86 39.1 Apocorophium sp. 58 29.1 Amphicteis floridus 11 4.3 Amphicteis floridus 11 4.7 Gammarus tigrinus 50 16.6 Eteone heteropoda 28 9.9 Gammarus tigrinus 51 16.9 Tanypus neopunctipennis 4 1.8 Streblospio benedicti 10 5.0 Tanypus neopunctipennis 5 2.0 Tanypus neopunctipennis 9 3.8 Marenzelleria viridis 30 10.0 Candonidae sp. 22 7.7 Littoridinops sp. 28 9.3 Littoridinops sp. 2 0.9 Eteone heteropoda 8 4.0 Tanytarsus sp. 5 2.0 MUDDY CREEK (CONTROL) DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Tubificoides sp. 229 65.6 Gammarus tigrinus 67 25.5 Chironomus decorus 469 73.6 Macoma balthica 160 29.1 Macoma balthica 153 38.3 Macoma balthica 309 70.1 Amphicteis floridus 60 18.4 Chironomus decorus 153 41.2 Cyprideis littoralis 42 12.0 Mediomastus ambiseta 37 14.1 Amphicteis floridus 60 9.4 Mediomastus ambiseta 135 24.6 Mediomastus ambiseta 73 18.3 Chironomus decorus 55 12.5 Chironomus decorus 54 16.6 Streblospio benedicti 89 24.0 Mediomastus ambiseta 40 11.5 Chironomus decorus 23 8.7 Procladius sp. 36 5.7 Chironomus decorus 44 8.0 Chironomus decorus 38 9.5 Streblospio benedicti 55 12.5 Littoridinops tenuipes 33 10.1 Cyprideis littoralis 34 9.2 Apocorophium lacustre 11 3.2 Amphicteis floridus 19 7.2 Chironomus plumulosus 32 5.0 Cyprideis littoralis 42 7.7 Apedilum sp. 34 8.5 Macoma tenta 6 1.4 Macoma balthica 32 9.8 Mediomastus ambiseta 25 6.7 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus decorus 65 33.2 Apedilum sp. 29 31.9 Chironomus decorus 92 22.0 Chironomus decorus 61 43.9 Macoma balthica 415 31.0 Chironomus decorus 116 28.2 Gammarus mucronatus 53 40.8 Chironomus decorus 419 76.3 Macoma balthica 55 28.1 Chironomus decorus 28 30.8 Polydora comuta 76 18.2 Macoma tenta 34 24.4 Chironomus decorus 292 21.8 Macoma balthica 83 20.1 Chironomus decorus 31 23.8 Macoma tenta 18 3.3 Macoma tenta 29 14.8 Polydora comuta 16 17.6 Macoma balthica 58 13.9 Macoma balthica 11 7.9 Mediomastus ambiseta 18 13.7 Mediomastus ambiseta 78 18.9 Parachironomus sp. 11 8.5 Streblospio benedicti 14 2.6 Mediomastus ambiseta 13 6.6 Littoridinops sp. 6 6.6 Streblospio benedicti 33 7.9 Cyprideis littoralis 9 6.5 Streblospio benedicti 83 6.2 Macoma tenta 38 9.2 Gammarus tigrinus 10 7.7 Polydora comuta 11 2.0 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus sp. 367 62.0 Chironomus decorus 162 53.3 Marenzelleria viridis 83 40.1 Gammarus tigrinus 118 23.6 Chironomus decorus 356 77.9 Gammarus tigrinus 21 34.4 Chironomus decorus 431 86.5 Streblospio benedicti 49 8.3 Marenzelleria viridis 46 15.1 Gammarus tigrinus 33 15.9 Macoma balthica 77 15.4 Streblospio benedicti 22 4.8 Eteone heteropoda 6 9.8 Tanypus neopunctipennis 31 6.2 Naididae sp. w/o hair 40 6.8 Macoma tenta 30 9.9 Streblospio benedicti 16 7.7 Apocorophium sp. 66 13.2 Littoridinops sp. 18 3.9 Littoridinops tenuipes 6 9.8 Streblospio benedicti 14 2.8 Macoma tenta 31 5.2 Macoma balthica 25 8.2 Apocorophium sp. 15 7.2 Gammarus mucronatus 59 11.8 Corixidae sp. 17 3.7 Edotia montosa 4 6.6 Gammarus tigrinus 4 0.8 III-G-92 Table III-G4 (continued). HUDDLES CUT UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - - - - - - - - - - Amphicteis floridus Monopylephorus sp. Gammanis tigrinus Bezzia/Palpomyia complex 120 62 20 18 46.9 24.2 7.8 7.0 Cyprideis littoralis Laeonereis culven Naididae sp. Chironomus decorus 9 6 5 5 19.6 13.0 10.9 10.9 Amphicteis floridus Laeonereis culven Tanytarsus sp. 1 Naididae sp. 68 41 40 33 22.7 13.7 13.3 11.0 not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Cyprideis littoralis Laeonereis culven Amphicteis floridus Apocorophium lacustre 77 7 6 5 64.7 5.9 5.0 4.2 Chironomus decorus Cyprideis littoralis Laeonereis culven Amphicteis floridus 104 38 10 8 52.0 19.0 5.0 4.0 Chironomus decorus Cyprideis littoralis Goeldichironomus devineyae Heteromastus filiformis 77 28 14 7 52.4 19.0 9.5 4.8 Chironomus decorus Apocorophium sp. Glyptotendipes sp. - 137 12 1 - 91.3 8.0 0.7 - Cyprideis littoralis Heteromastus filifonnis Macoma tenta Bezzia/Palpomyia complex 54 50 23 15 32.5 30.1 13.9 9.0 Heteromastus filiformis Cyprideis littoralis Neanthes succinea Mediomastus ambiseta 182 52 36 20 49.2 14.1 9.7 5.4 Heteromastus filiformis Chironomus decorus Naididae sp. w/o hair Neanthes succinea 144 47 28 26 45.0 14.7 8.8 8.1 Macoma tenta Chironomus decorus Naididae sp. w/o hair Gammarus tigrinus 120 91 50 50 23.6 17.9 9.8 9.8 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Tanytarsus sp. Amphicteis floridus Macoma tenta Bezzia/Palpomyia complex 19 12 11 9 19.6 12.4 11.3 9.3 Tubificoides heterochaetus Tanytarsus sp. Amphicteis floridus Calanoida sp. 251 81 54 43 42.1 13.6 9.1 7.2 Cyprideis littoralis Chironomus decorus Laeonereis culven Macoma tenta 146 39 24 19 49.0 13.1 8.1 6.4 Balanus improvisus Neanthes succinea Heteromastus filiformis Macoma tenta 150 50 35 19 39.7 13.2 9.3 5.0 Chironomus decorus Tanypus neopunctipennis Littoridinops sp. Cyprideis littoralis 379 57 39 38 70.6 10.6 7.3 7.1 Mediomastus ambiseta Heteromastus filiformis Neanthes succinea Macoma tenta 105 55 22 11 44.7 23.4 9.4 4.7 Chironomus decorus Bezzia/Palpomyia complex Tanypus neopunctipennis Polypedilum sp. 164 4 3 2 92.1 2.2 1.7 1.1 1 M. t HUDDLES CUT DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - - - - - - - - - Chironomus decorus Amphicteis floridus Macoma tenta Gammarus tigrinus 91 24 22 20 33.5 8.8 8.1 7.4 Chironomus decorus Amphicteis floridus Marenzelleria viridis Polydora comuta 463 440 111 71 35.9 34.1 8.6 5.5 Mediomastus ambiseta Macoma balthica Amphicteis floridus Macoma tenta 144 106 34 28 28.7 21.1 6.8 5.6 not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus decorus Gammanis tigrinus Streblospio benedicti Littoridinops tenuipes 356 134 36 30 55.1 20.7 5.6 4.6 Nematoda sp. Laeonereis culveri Polydora comuta Naididae sp. 220 72 60 27 44.3 14.5 12.1 5.4 Naididae sp. Nematoda sp. Neanthes succinea Macoma tenta 116 64 8 6 52.7 29.1 3.6 2.7 Marenzelleria viridis Chironomus decorus Laeonereis culven Nemertea sp. 208 28 17 10 66.7 9.0 5.4 3.2 Nematoda sp. Turbellaria sp. Marenzelleria viridis Heteromastus filiformis 416 288 147 78 32.8 22.7 11.6 6.2 Chironomus decorus Streblospio benedicti Heteromastus filiformis Eteone heteropoda 89 80 74 63 17.7 15.9 14.7 12.5 Streblospio benedicti Mediomastus ambiseta Naididae sp. w/o hair Gammarus tigrinus 412 328 226 196 20.9 16.6 11.5 10.0 Nematoda sp. Grania sp. Lepidactylus dytiscus Marenzelleria viridis 500 252 96 92 45.0 22.7 8.6 8.3 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Nematoda sp. Aulodrilus limnobius Ostracoda sp. Lepidactylus dytiscus 500 276 124 84 50.2 27.7 12.4 8.4 Nematoda sp. Chironomus decorus Marenzelleria viridis Cyprideis littoralis 124 97 97 66 18.3 14.3 14.3 9.8 Chironomus decorus Cyprideis littoralis Amphicteis floridus Rangia cuneata 462 131 120 55 49.8 14.1 12.9 5.9 Marenzelleria viridis Haustorius sp. Lepidactylus dytiscus Naididae sp. w/o hair 475 62 42 40 59.8 7.8 5.3 5.0 Naididae sp. w/o hair Marenzelleria viridis Chironomus decorus Neanthes succinea 60 12 9 9 55.6 11.1 8.3 8.3 Lepidactylus dytiscus Heteromastus filifonnis Neanthes succinea Eteone heteropoda 34 18 18 14 29.8 15.8 15.8 12.3 Naididae sp. w/o hair Marenzelleria viridis Tanytarsus sp. Gammarus tigrinus 73 26 5 4 60.3 21.5 4.1 3.3 Y W W ct 0' Q 0 W Ce i_ LLJ (/) 11 S d 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - not monitored - - - - - - - - - - - Amphicteis floridus Apocorophium sp. Gammarus tigrinus Streblospio benedicti 379 283 205 180 29.4 22.0 15.9 14.0 Tanytarsus sp. Littoridinops tenuipes Cyprideis littoralis Amphicteis floridus 448 416 300 260 20.8 19.3 13.9 12.1 Apocorophium sp. Gammarus tigrinus Apocorophium louisianum Streblospio benedicti 109 81 44 32 34.4 25.6 13.9 10.1 Amphicteis floridus Streblospio benedicti Tanytarsus sp. Gammarus tigrinus 333 256 205 122 29.0 22.3 17.8 10.6 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Amphicteis floridus Gammanis tigrinus Apocorophium sp. Mytilopsis leucophaeata 484 183 54 29 56.7 21.5 6.3 3.4 Amphicteis floridus Cyprideies littoralis Gammarus tigrinus Littoridinops sp. 500 281 249 45 40.3 22.7 20.1 3.6 Amphicteis floridus Gammarus tigrinus Cyprideis littoralis Littoridinops sp. 500 317 236 149 37.5 23.7 17.7 11.2 Gammarus tigrinus Streblospio benedicti Littoridinops sp. Apocorophium lacustre 400 78 64 32 57.6 11.2 9.2 4.6 Gammarus tigrinus Chironomus decorus Littoridinops sp. Dero sp. 176 78 42 26 39.7 17.6 9.5 5.9 Gammarus tigrinus Apocorophium sp. Apocorophium louisianum Candonidae sp. 172 130 78 22 36.4 27.5 16.5 4.7 Amphicteis floridus Chironomus decorus Gammanis tigrinus Tanytarsus sp. 98 45 42 5 42.2 19.4 18.1 2.2 Y w 11 w 0 II cn W Z 0 dp 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - - - - not monitored - - - - - not monitored - - - - - - - - not monitored - - - - - - - - Macoma balthica Marenzelleria viridis Mediomastus ambiseta Eteone heteropoda 308 207 16 14 52.3 35.1 2.7 2.3 Mediomastus ambiseta Macoma tenta Macoma balthica Streblospio benedicti 42 28 20 18 34.1 22.8 16.3 14.6 Streblospio benedicti Chironomus decorus Mediomastus ambiseta Macoma balthica 109 85 23 14 38.1 29.7 8.0 4.9 Chironomus decorus Tanypus neopunctipennis Macoma tenta Macoma balthica 197 63 58 34 46.4 14.8 13.6 8.0 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus sp. Streblospio benedicti Amphicteis floridus Gammanis tigrinus 500 79 12 12 79.6 12.6 1.9 1.9 Chironomus decorus Marenzelleria viridis Macoma tenta Gammarus tigrinus 102 96 65 31 26.7 25.1 17.0 8.1 Chironomus decorus Amphicteis floridus Streblospio benedicti Marenzelleria viridis 184 170 150 75 24.8 22.9 20.2 10.1 Mediomastus ambiseta Macoma tenta Marenzelleria viridis Macoma balthica 212 111 84 83 32.2 16.9 12.8 12.6 Chironomus decorus Gammanis tigrinus Littoridinops sp. Procladius sp. 406 36 23 8 83.0 7.4 4.7 1.6 Mediomastus ambiseta Streblospio benedicti Gammarus tigrinus Chironomus decorus 399 41 26 11 75.0 7.7 4.9 2.1 Chironomus decorus Macoma tenta Streblospio benedicti Procladius sp. 321 13 8 5 90.2 3.7 2.2 1.4 III-G-93 Table III-G4 (continued). DCUT11 UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - Gammarus tigrinus Amphicteis floridus Apocorophium sp. Littoridinops tenuipes 500 414 155 150 35.1 29.1 10.9 10.5 Littoridinops tenuipes Gammarus tigrinus Amphicteis floridus Cyprideis littoralis 500 458 368 312 22.8 20.9 16.8 14.2 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Littoridinops sp. Cyprideis littoralis Amphicteis floridus Berosus sp. 464 111 63 42 65.1 15.6 8.8 5.9 Cyprideis littoralis Gammarus tigrinus Amphicteis floridus Littoridinops sp. 407 263 224 190 28.6 18.5 15.8 13.4 Gammarus tigrinus Cyprideis littoralis Littoridinops sp. Amphicteis floridus 298 68 28 26 66.8 15.2 6.3 5.8 Gammarus tigrinus Amphicteis floridus Cyprideis littoralis Littoridinops sp. 356 148 107 81 45.4 18.9 13.6 10.3 Gammarus tigrinus Amphicteis floridus Candonidae sp. Dicrotendipes nervosus 134 95 88 73 21.9 15.5 14.4 11.9 Gammarus tigrinus Littoridinops tenuipes Apocorophium sp. Cyprideis littoralis 396 389 236 118 25.8 25.3 15.4 7.7 Dicrotendipes nervosus Littoridinops sp. Gammarus tigrinus Tanytarsus sp. 26 23 13 10 25.0 22.1 12.5 9.6 DCUT11 DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - Gammarus tigrinus Apocorophium sp. Apocorophium louisianum Amphicteis floridus 429 317 57 15 48.9 36.1 6.5 1.7 Amphicteis floridus Gammarus tigrinus Chironomus decorus Dicrotendipes nervosus 447 400 165 114 28.4 25.4 10.5 7.2 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Amphicteis floridus Gammarus tigrinus Dicrotendipes nervosus Apocorophium sp. 358 332 196 64 32.6 30.2 17.8 5.8 Gammarus tigrinus Amphicteis floridus Marenzellena viridis Apocorophium lacustre 108 90 11 8 43.5 36.3 4.4 3.2 Gammarus tigrinus Amphicteis floridus Littoridinops sp. Mytilopsis leucophaeta 440 422 186 50 37.0 35.5 15.7 4.2 Gammarus tigrinus Apocorophium sp. Apocorophium lacustre Tanytarsus sp. 462 456 318 102 29.8 29.5 20.5 6.6 Amphicteis floridus Gammarus tigrinus Littoridinops sp. Cyprideis littoralis 224 203 20 18 41.3 37.5 3.7 3.3 Gammarus tigrinus Apocorophium sp. Apocorophium lacustre Dicrotendipes nervosus 500 438 387 320 23.8 20.8 18.4 15.2 Amphicteis floridus Gammarus tigrinus Dicrotendipes nervosus Tanytarsus sp. 218 124 100 66 34.9 19.9 16.0 10.6 DCUT19 (CONTROL) UPSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - Littoridinops tenuipes Chironomus decorus Amphicteis floridus Gammarus tigrinus 182 146 116 70 21.4 17.2 13.6 8.2 Littoridinops tenuipes Tanytarsus sp. Chironomus decorus Bezzia/Palpomyia complex 497 185 157 55 43.1 16.0 13.6 4.8 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Amphicteis floridus Littoridinops sp. Tanytarsus sp. Cyprideis littoralis 44 36 18 12 25.9 21.2 10.6 7.1 Amphicteis floridus Littoridinops sp. Cyprideis littoralis Nematoda sp. 297 295 160 128 25.4 25.2 13.7 11.0 Candonidae sp. Tanytarsus sp. Amphicteis floridus Littoridinops sp. 256 160 136 116 22.8 14.2 12.1 10.3 Candonidae sp. Apocorophium sp. Littoridinops sp. Apocorophium louisianum 290 136 67 37 46.0 21.6 10.6 5.9 Tanytarsus sp. Candonidae sp. Amphicteis floridus Littoridinops sp. 290 177 155 61 36.4 22.2 19.5 7.7 Apocorophium sp. Littoridinops tenuipes Candonidae sp. Chironomus decorus 128 83 35 28 31.8 20.6 8.7 7.0 Chironomus decorus Littoridinops sp. Tanytarsus sp. Dicrotendipes nervosus 234 118 85 57 32.1 16.2 11.6 7.8 • DCUT19 (CONTROL) DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - Hargeria rapax Gammarus tigrinus Nematoda sp. Cyathura polita 500 424 140 68 36.0 30.5 10.1 4.9 Gammarus tigrinus Naididae sp. w/o hair Hargeria rapax Littoridinops tenuipes 500 428 128 95 34.6 29.6 8.9 6.6 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total Amphicteis floridus Gammarus tigrinus Candonidae sp. Chironomus sp. 395 296 171 165 19.7 14.7 8.5 8.2 Gammarus tigrinus Amphicteis floridus Apocorophium lacustre Cyprideis littoralis 475 147 63 45 50.8 15.7 6.7 4.8 Gammarus tigrinus Amphicteis floridus Littoridinops sp. Cyprideis littoralis 500 500 282 234 18.9 18.9 10.7 8.9 Gammarus tigrinus Naididae sp. w/o hair Apocorophium sp. Hargeria rapax 433 95 31 15 68.1 14.9 4.9 2.4 Gammarus tigrinus Candonidae sp. Littoridinops sp. Chironomus decorus 476 158 84 79 49.9 16.6 8.8 8.3 Hargeria rapax Gammarus tigrinus Apocorophium sp. Amphicteis floridus 500 435 344 78 29.9 26.0 20.6 4.7 Gammarus tigrinus Amphicteis floridus Hargeria rapax Dicrotendipes nervosus 500 423 404 228 24.5 20.7 19.8 11.2 III-G-94 Table III-G4 (concluded). 1998 1999 2000 2001 2002 2003 2004 2005 -IDominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 0 0' 2007 2008 2009 2010 2011 2012 2013 2014 I- Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Z 0 a not monitored - - not monitored - - not monitored - - not monitored - - Amphicteis floridus 436 23.4 Candonidae sp. 500 26.2 Nematoda sp. 293 25.3 Amphicteis floridus 500 25.7 V W - - - - - - - - - - - - Littoridinops tenuipes 416 22.3 Chironomus decorus 484 25.4 Candonidae sp. 227 19.6 Chironomus decorus 500 25.7 WCe Gammarus tigrinus 308 16.5 Tanytarsus sp. 332 17.4 Amphicteis floridus 193 16.6 Gammarus tigrinus 221 11.4 W V) Dicrotendipes nervosus 152 8.2 Littoridinops tenuipes 276 14.5 Littoridinops tenuipes 147 12.7 Littoridinops tenuipes 191 9.8 Ce d 2015 2016 2017 2018 2019 2020 2021 U M Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % 0 Amphicteis floridus 199 33.2 Gammarus tigrinus 386 29.5 Amphicteis floridus 254 43.8 Cyprideis littoralis 126 34.5 Candonidae sp. 71 33.6 Amphicteis floridus 126 59.4 Amphicteis floridus 26 21.3 M Gammarus tigrinus 115 19.2 Amphicteis floridus 247 18.9 Candonidae sp. 74 12.8 Candonidae sp. 95 26.0 Gammarus tigrinus 58 27.5 Gammarus tigrinus 28 13.2 Dicrotendipes nervosus 24 19.7 Candonidae sp. 54 9.0 Candonidae sp. 221 16.9 Gammarus tigrinus 70 12.1 Gammarus tigrinus 55 15.1 Naididae sp. w/o hair 26 12.3 Cyprideis littoralis 22 10.4 Chironomus decorus 12 9.8 Chironomus sp. 44 7.3 Cyprideis littoralis 126 9.6 Naididae sp. w/o hair 64 11.0 Littoridinops sp. 51 14.0 Tanytarsus sp. 15 7.1 Candonidae sp. 20 9.4 Gammarus tigrinus 12 9.8 DUCK CREEK (CONTROL) DOWNSTREAM 1998 1999 2000 2001 2002 2003 2004 2005 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - not monitored - - 2007 2008 2009 2010 2011 2012 2013 2014 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % not monitored - - not monitored - - not monitored - - not monitored - - Macoma balthica 456 37.8 Mediomastus ambiseta 398 54.5 Gammarus mucronatus 123 28.1 Chironomus decorus 500 79.0 Marenzelleria viridis 338 28.0 Chironomus decorus 167 22.9 Macoma balthica 72 16.5 Streblospio benedicti 56 8.8 Mediomastus ambiseta 166 13.8 Macoma balthica 49 6.7 Gammarus tigrinus 70 16.0 Littoridinops tenuipes 15 2.4 Chironomus decorus 81 6.7 Parachironomus sp. 23 3.2 Chironomus decorus 47 10.8 Macoma tenta 13 2.1 2015 2016 2017 2018 2019 2020 2021 Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Dominant Species Total % Chironomus sp. 489 82.2 Gammanis tigrinus 35 23.8 Amphicteis floridus 244 40.6 Gammanis tigrinus 384 38.5 Procladius sp. 293 35.3 Mediomastus ambiseta 331 74.4 Chironomus decorus 132 46.3 Tanytarsus sp. 30 5.0 Leptocheirus plumulosus 35 23.8 Procladius sp. 55 9.2 Chironomus decorus 156 15.6 Cyprideis littoralis 219 26.4 Streblospio benedicti 54 12.1 Tanytarsus sp. 82 28.8 Tanypus neopunctipennis 29 4.9 Coelotanypus sp. 14 9.5 Tubificoides heterochaetus 54 9.0 Mediomastus ambiseta 104 10.4 Chironomus decorus 172 20.7 Chironomus decorus 17 3.8 Procladius sp. 30 10.5 Streblospio benedicti 14 2.4 Macoma tenta 12 8.2 Apocorophium sp. 42 7.0 Balanus improvisus 101 10.1 Tanypus neopunctipennis 44 5.3 Gammarus tigrinus 11 2.5 Amphicteis floridus 11 3.9 III-G-95 Table III-G5. Benthic macroinvertebrate community structure data for ponar grab samples collected at upstream and downstream stations in May during years 1998-2005 and 2007-2021. A "-" indicates the creek was not monitored that year. Pre- and post -Mod Alt L collection years for impact creeks are separated by bold vertical lines. Benthic collections occurred in June for 2010. Summary pre and post Mod Alt L are shown in Table II-05. An "a" refers to only those taxa for which sensitivity values are available were used in EBI calculations. Note: sensitivity values provided by NCDWR. Station community structure data by year 1998 1999 2000 2001 2002 2003 2004 2005 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 JACOBS CREEK JACKS CREEK SCUT 1 (CONTROL) BROOMFIELD SWAMP CREEK Total abundance - - - - - - - - - - - - - - - - - - - - 347 87 297 Total taxa - - - - - - - - - - - - - - - - - - - - 11 5 12 D Total taxa with sensitivity values - - - - - - - - - - - - - - - - - - - - 9 5 9 Shannon -Wiener Index - - - - - - - - - - - - - - - - - - - - 1.75 1.21 1.76 E B la - - - - - - - - - - - - - - - - - - - - 1.04 1.22 1.03 Total abundance - - - - - - - - - - - - - - - - - - - - 356 144 37 Total taxa - - - - - - - - - - - - - - - - - - - - 14 10 11 oTotal taxa with sensitivity values - - - - - - - - - - - - - - - - - - - - 10 10 10 Shannon -Wiener Index - - - - - - - - - - - - - - - - - - - - 1.30 1.16 2.02 E B la - - - - - - - - - - - - - - - - - - - - 1.02 1.91 1.20 Total abundance - - - - - - - - - - - - - - - - - - - - 509 334 389 Total taxa - - - - - - - - - - - - - - - - - - - - 18 7 16 D Total taxa with sensitivity values - - - - - - - - - - - - - - - - - - - - 14 6 11 Shannon -Wiener Index - - - - - - - - - - - - - - - - - - - - 1.72 0.99 2.02 E B la - - - - - - - - - - - - - - - - - - - - 1.23 1.74 1.05 Total abundance - - - - - - - - - - - - - - - - - - - - 219 92 277 Total taxa - - - - - - - - - - - - - - - - - - - - 16 8 8 oTotal taxa with sensitivity values - - - - - - - - - - - - - - - - - - - - 11 6 7 Shannon -Wiener Index - - - - - - - - - - - - - - - - - - - - 2.13 1.74 1.00 E B la - - - - - - - - - - - - - - - - - - - - 1.25 1.77 1.96 Total abundance 215 515 1,121 1,356 295 615 549 463 - - - - 549 255 389 940 353 90 549 636 123 142 188 Totaltaxa 19 8 19 17 13 17 30 18 - - - - 19 5 13 24 18 14 19 24 15 12 15 D Total taxa with sensitivity values 17 5 16 13 12 14 26 15 - - - - 17 4 11 20 16 12 17 22 12 9 12 Shannon -Wiener Index 2.14 0.72 1.82 1.77 1.26 0.97 2.22 2.01 - - - - 2.33 0.78 1.62 2.19 2.20 2.00 2.22 1.83 2.17 1.99 1.67 E B la 1.85 2.00 1.57 1.65 1.67 1.48 1.80 1.58 - - - - 1.57 1.07 1.41 1.63 1.84 1.99 1.79 1.75 1.43 1.44 1.18 Total abundance 221 274 314 398 1246 1639 719 1013 - - - - 1090 203 427 183 831 315 372 436 192 473 564 Totaltaxa 18 17 11 16 18 15 22 18 - - - - 23 15 19 23 25 15 18 14 10 19 10 oTotal taxa with sensitivity values 16 15 11 15 17 14 21 17 - - - - 22 11 17 21 21 14 16 14 8 16 9 Shannon -Wiener Index 2.27 2.07 1.25 1.94 1.64 1.55 2.18 1.59 - - - - 2.39 1.24 1.54 2.51 2.08 1.55 1.91 1.89 0.96 1.80 0.80 E B la 1.40 2.13 1.19 1.95 1.57 1.56 1.71 1.29 - - - - 1.46 1.11 1.41 1.57 1.42 2.33 2.10 2.06 1.17 1.93 1.20 Total abundance - - - - - - - - - - - - 395 678 1,277 559 1,163 59 990 493 341 170 174 Total taxa - - - - - - - - - - - - 18 18 18 15 23 16 22 19 15 21 12 D Total taxa with sensitivity values - - - - - - - - - - - - 17 17 13 13 18 14 19 16 11 19 12 Shannon -Wiener Index - - - - - - - - - - - - 2.10 1.92 1.98 1.89 2.14 2.42 2.15 2.09 1.92 2.48 1.45 E B la - - - - - - - - - - - - 1.66 2.22 2.27 1.35 2.00 2.00 1.91 1.71 1.40 1.78 1.13 Total abundance - - - - - - - - - - - - 628 608 592 309 679 213 877 708 616 370 480 Total taxa - - - - - - - - - - - - 24 12 22 18 23 21 21 19 14 18 15 oTotal taxa with sensitivity values - - - - - - - - - - - - 21 11 19 16 21 21 19 17 11 18 15 Shannon -Wiener Index - - - - - - - - - - - - 2.1 1.58 1.78 1.77 1.55 1.87 2.07 2.06 0.90 1.88 0.47 E B la - - - - - - - - - - - - 1.58 2.17 1.57 1.47 1.43 2.27 2.02 1.68 1.30 2.02 1.11 III-G-96 Table III-G5 (concluded). Station community structure data by year 1998 1999 2000 2001 2002 2003 2004 2005 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 LONG CREEK (CONTROL) LITTLE CREEK (CONTROL) DRINKWATER CREEK PA2 (CONTROL) D Total abundance Total taxa Total taxa with sensitivity values Shannon -Wiener Index E Bla - - - - - - - - - - - - 784 - - - - - - - - - - - - 18 - - - - - - - - - - - - 16 - - - - - - - - - - - - 2.26 - - - - - - - - - - - - 1.89 586 7 6 1.07 2.31 414 14 12 1.54 2.87 305 13 12 1.68 1.75 563 18 15 1.35 1.42 554 14 11 1.23 1.49 395 19 17 1.55 2.02 507 16 15 2.28 1.54 589 18 14 1.48 1.42 363 15 14 2.17 2.19 374 14 13 1.35 1.18 Total abundance - - - - - - - - - - - - 439 776 450 174 783 970 1217 1392 666 137 762 Total taxa - - - - - - - - - - - - 19 12 16 11 27 23 27 25 18 18 21 oTotal taxa with sensitivity values - - - - - - - - - - - - 16 10 14 11 24 22 25 24 15 16 19 Shannon -Wiener Index - - - - - - - - - - - - 2.31 1.15 1.84 1.71 2.35 2.16 1.88 2.46 1.46 1.95 2.11 EBIa - - - - - - - - - - - - 1.80 2.39 2.82 1.49 1.89 2.06 1.99 1.65 1.38 2.04 1.47 Total abundance - - - - - - - - - - - - 1,236 1,267 829 973 265 363 930 767 530 242 343 Total taxa - - - - - - - - - - - - 21 22 23 22 22 17 19 18 11 15 20 D Total taxa with sensitivity values - - - - - - - - - - - - 19 19 21 20 19 16 16 17 9 15 18 Shannon -Wiener Index - - - - - - - - - - - - 2.32 2.08 1.58 2.32 2.27 1.87 1.80 1.75 1.97 2.14 1.78 EBIa - - - - - - - - - - - - 1.88 1.70 1.64 1.59 1.71 2.10 1.95 1.68 1.76 1.80 1.74 Total abundance - - - - - - - - - - - - 1089 810 1140 1113 161 354 483 1138 673 390 508 Total taxa - - - - - - - - - - - - 21 16 22 19 13 17 17 23 16 23 12 oTotal taxa with sensitivity values - - - - - - - - - - - - 20 14 20 16 12 15 16 22 12 22 11 Shannon -Wiener Index - - - - - - - - - - - - 2.33 1.34 2.46 1.85 2.18 1.45 2.00 2.43 1.03 2.38 0.59 EBIa - - - - - - - - - - - - 1.68 2.22 1.86 1.68 1.57 2.23 2.03 1.67 1.16 1.84 1.09 Total abundance - - - - - - - - - - - - 610 1,394 1,865 366 522 625 1,158 1,297 732 69 534 Total taxa - - - - - - - - - - - - 15 11 23 11 12 17 18 19 16 6 15 D Total taxa with sensitivity values - - - - - - - - - - - - 14 9 18 9 11 14 15 16 13 4 14 Shannon -Wiener Index - - - - - - - - - - - - 1.23 1.65 2.27 1.94 1.51 1.74 2.11 1.85 1.95 1.08 1.32 E Bla - - - - - - - - - - - - 1.95 1.78 1.71 1.45 1.75 1.88 1.38 1.64 1.49 2.00 1.49 Total abundance - - - - - - - - - - - - 619 533 2023 889 2018 478 1219 988 601 354 539 Total taxa - - - - - - - - - - - - 22 16 28 19 25 18 20 25 11 17 11 oTotal taxa with sensitivity values - - - - - - - - - - - - 21 15 25 18 22 17 18 23 10 15 11 Shannon -Wiener Index - - - - - - - - - - - - 2.01 1.39 2.34 1.81 2.40 2.08 2.24 2.07 0.75 2.27 0.38 E Bla - - - - - - - - - - - - 1.98 1.84 1.71 1.36 1.68 1.84 1.90 1.72 1.24 1.90 1.06 Total abundance - - - - - - - - - - - - 503 376 941 719 633 86 822 561 245 401 510 Total taxa - - - - - - - - - - - - 18 14 22 25 19 12 25 32 10 19 8 D Total taxa with sensitivity values - - - - - - - - - - - - 17 12 20 22 16 11 21 28 9 18 7 Shannon -Wiener Index - - - - - - - - - - - - 2.04 1.49 1.65 1.57 1.91 1.87 2.27 2.56 1.11 1.84 0.52 EBIa - - - - - - - - - - - - 2.31 1.36 1.89 1.49 1.45 2.05 1.90 1.79 1.22 2.04 1.18 Total abundance - - - - - - - - - - - - 216 769 363 273 941 176 296 351 532 70 511 Total taxa - - - - - - - - - - - - 11 15 16 14 14 14 17 22 16 9 10 oTotal taxa with sensitivity values - - - - - - - - - - - - 10 12 15 13 12 12 16 21 15 9 10 Shannon -Wiener Index - - - - - - - - - - - - 1.25 1.26 1.71 1.29 1.52 2.12 2.03 2.66 0.94 1.67 0.54 EBIa - - - - - - - - - - - - 1.76 1.72 1.44 1.29 1.27 1.66 2.03 1.85 1.28 1.74 1.06 III-G-97 Table III-G5 (continued). Station community structure data by year 1998 1999 2000 2001 2002 2003 2004 2005 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 PORTER CREEK HUDDLES CREEK MUDDY CREEK (CONTROL) TOOLEY CREEK Total abundance 475 320 459 937 - - - - - - - 138 363 155 942 232 232 65 252 198 360 50 316 Totaltaxa 17 13 11 24 - - - - - - - 13 19 11 21 23 19 13 19 18 16 12 14 D Total taxa with sensitivity values 16 10 10 23 - - - - - - - 10 18 10 19 21 16 12 16 16 15 11 13 Shannon -Wiener Index 2.04 1.35 0.49 1.81 - - - - - - - 2.04 2.30 1.40 1.86 2.43 2.25 2.07 2.39 2.21 2.11 2.31 1.22 EBIa 2.09 2.03 1.16 1.58 - - - - - - - 1.28 1.76 1.15 1.80 1.51 1.73 2.03 2.00 1.52 1.51 1.86 1.25 Total abundance 919 270 743 1,088 - - - - - - - 511 416 1,415 877 812 995 285 669 1,056 355 272 511 Totaltaxa 20 18 11 25 - - - - - - - 18 18 17 24 16 20 16 23 22 14 16 11 o Total taxa with sensitivity values 19 15 9 24 - - - - - - - 16 17 16 22 14 19 15 22 20 13 15 11 Shannon -Wiener Index 1.68 2.31 1.37 1.75 - - - - - - - 2.16 1.93 1.57 1.54 1.33 1.66 2.01 2.28 2.21 1.00 1.28 0.68 EBIa 1.45 2.07 1.22 1.77 - - - - - - - 1.59 1.89 1.42 1.46 1.54 1.41 1.86 1.75 1.79 1.16 1.85 1.16 Total abundance 1,191 149 546 51 75 114 46 616 291 35 191 67 234 203 160 49 235 307 284 302 220 199 256 Totaltaxa 21 12 9 6 11 8 11 20 11 9 19 7 12 7 10 8 13 16 15 12 7 12 10 D Total taxa with sensitivity values 20 10 6 5 10 6 9 17 10 8 17 6 10 6 8 5 11 13 14 10 6 11 8 Shannon -Wiener Index 1.77 1.77 0.82 1.47 1.57 1.54 1.93 2.01 1.48 1.86 2.37 1.02 1.21 1.25 1.14 1.23 1.39 1.89 2.19 1.87 0.88 1.51 0.71 EBIa 2.05 2.00 1.17 1.51 1.73 1.33 1.32 1.69 1.14 1.32 1.57 1.36 1.23 1.65 1.05 1.41 1.19 1.50 2.02 1.55 1.07 1.94 1.20 Total abundance 349 263 637 556 400 442 326 371 196 91 418 139 1,329 412 162 549 592 304 207 500 457 61 498 Totaltaxa 15 17 16 25 18 12 21 16 15 12 26 17 24 19 16 19 20 15 19 20 15 15 14 o Total taxa with sensitivity values 14 16 14 24 17 11 19 15 14 10 23 16 22 18 14 17 18 13 17 18 13 13 13 Shannon -Wiener Index 1.25 2.45 1.08 2.32 2.03 1.03 2.54 1.81 1.88 1.76 2.52 1.81 2.17 2.08 2.22 1.19 1.58 1.60 2.12 2.31 1.02 2.25 0.63 EBIa 1.42 1.81 1.28 1.72 1.87 1.39 1.64 1.24 1.47 2.01 1.45 1.63 1.63 1.44 1.74 1.28 1.31 1.53 2.02 1.91 1.24 1.94 1.12 Total abundance - 256 46 300 - - - - 119 200 147 150 166 370 325 509 97 596 298 378 537 235 178 Totaltaxa - 17 10 20 - - - - 18 13 15 3 15 14 15 22 17 23 21 25 11 17 9 D Total taxa with sensitivity values - 13 9 18 - - - - 15 12 11 3 14 13 13 20 15 18 19 24 9 16 9 Shannon -Wiener Index - 1.69 2.27 2.41 - - - - 1.56 1.59 1.61 0.32 1.81 1.83 1.95 2.47 2.52 2.14 1.93 2.30 1.07 1.79 0.43 EBIa - 1.98 1.57 1.72 - - - - 1.63 1.12 1.34 1.11 1.57 1.44 1.29 1.65 1.85 1.65 1.49 1.81 1.26 1.76 1.09 Total abundance - 272 1291 502 - - - - 646 500 220 312 1268 530 1980 1112 996 676 928 794 108 114 121 Totaltaxa - 19 23 24 - - - - 19 13 11 17 21 19 20 19 7 22 24 18 8 11 8 oTotal taxa with sensitivity values - 17 19 19 - - - - 17 11 10 15 17 18 17 15 4 19 21 15 8 11 7 Shannon -Wiener Index - 2.4 1.8 2.4 - - - - 1.53 1.88 1.39 1.44 2.09 2.56 2.43 1.77 1.24 2.52 1.82 1.70 1.51 2.01 1.26 EBIa - 1.62 1.81 1.71 - - - - 1.56 1.29 1.13 2.23 1.96 1.66 1.49 2.03 3.08 1.85 1.53 2.15 1.49 2.44 1.55 Total abundance - - - - - - - - - - - - 1,387 2,136 317 1,150 847 1,240 1,335 695 443 472 232 Total taxa - - - - - - - - - - - - 22 20 15 21 18 16 18 15 21 14 11 D Total taxa with sensitivity values - - - - - - - - - - - - 20 17 14 18 13 12 14 13 17 12 10 Shannon -Wiener Index - - - - - - - - - - - - 2.14 2.30 1.84 2.01 1.53 1.73 1.74 1.61 2.08 1.76 1.68 E Bla - - - - - - - - - - - - 1.86 1.84 1.91 1.80 1.96 1.90 1.91 1.87 1.68 2.06 1.73 Total abundance - - - - - - - - - - - - 589 123 287 425 628 382 743 658 489 532 356 Total taxa - - - - - - - - - - - - 12 10 19 11 11 16 14 16 12 17 9 oTotal taxa with sensitivity values - - - - - - - - - - - - 12 9 17 11 10 14 13 15 11 14 8 Shannon -Wiener Index - - - - - - - - - - - - 1.23 1.72 1.87 1.62 0.79 2.00 2.03 2.00 0.72 1.10 0.49 EBIa - - - - - - - - - - - - 1.94 1.76 1.26 1.33 1.2 1.6 1.55 1.89 1.31 1.79 1.14 III-G-98 Table III-G5 (continued). Station community structure data by year 1998 1999 2000 2001 2002 2003 2004 2005 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 DUCK CREEK (CONTROL) DCUT19 (CONTROL) DCUT11 Total abundance - - - - - - - - - - - - - - 1,423 2,192 713 1,422 446 784 611 1,535 104 Total taxa - - - - - - - - - - - - - - 19 21 18 21 12 18 22 18 10 D Total taxa with sensitivity values - - - - - - - - - - - - - - 15 16 14 15 9 16 17 15 10 Shannon -Wiener Index - - - - - - - - - - - - - - 1.83 2.08 1.20 2.12 1.17 1.71 2.42 2.01 2.04 E Bla - - - - - - - - - - - - - - 2.01 1.88 1.88 1.84 2.00 1.95 1.80 1.99 1.82 Total abundance - - - - - - - - - - - - - - 878 1575 1099 248 1188 1548 542 2105 624 Total taxa - - - - - - - - - - - - - - 14 28 22 17 18 20 15 16 22 oTotal taxa with sensitivity values - - - - - - - - - - - - - - 11 25 17 14 14 18 13 15 17 Shannon -Wiener Index - - - - - - - - - - - - - - 1.31 2.19 1.88 1.54 1.52 1.78 1.55 1.96 1.91 E Bla - - - - - - - - - - - - - - 2.07 1.67 1.71 2.02 1.95 2.05 1.95 1.87 1.73 Total abundance - - - - - - - - - - - - - - 850 1,153 170 1,169 1,124 630 796 402 730 Total taxa - - - - - - - - - - - - - - 21 17 13 19 18 13 15 20 15 D Total taxa with sensitivity values - - - - - - - - - - - - - - 19 14 10 15 13 11 11 19 13 Shannon -Wiener Index - - - - - - - - - - - - - - 2.49 1.95 2.19 2.11 2.32 1.67 1.78 2.23 1.99 EBIa - - - - - - - - - - - - - - 1.71 1.73 1.88 1.98 1.96 2.12 2.03 2.00 1.63 Total abundance - - - - - - - - - - - - - - 1,388 1,446 2,007 935 2,641 636 953 1,672 2,040 Total taxa - - - - - - - - - - - - - - 20 24 27 21 24 17 13 25 19 oTotal taxa with sensitivity values - - - - - - - - - - - - - - 18 23 23 18 19 15 11 23 18 Shannon -Wiener Index - - - - - - - - - - - - - - 1.88 2.01 2.74 1.84 2.52 1.25 1.69 1.98 2.13 E Bla - - - - - - - - - - - - - - 1.78 1.71 1.75 2.03 1.71 1.86 1.73 1.88 1.71 Total abundance - - - - - - - - - - - - 1,865 1,908 1,173 1,944 600 1,307 580 365 211 212 122 Total taxa - - - - - - - - - - - - 17 15 19 15 16 19 11 8 15 9 13 D Total taxa with sensitivity values - - - - - - - - - - - - 15 12 14 12 11 15 8 6 12 7 11 Shannon -Wiener Index - - - - - - - - - - - - 2.20 1.93 2.14 2.09 2.17 2.15 1.75 1.63 1.94 1.34 2.27 E Bla - - - - - - - - - - - - 1.76 1.73 1.85 1.54 1.86 1.77 1.83 1.95 1.64 2.01 1.64 Total abundance - - - - - - - - - - - - 1,206 730 438 633 595 147 601 998 829 445 285 Total taxa - - - - - - - - - - - - 19 18 20 16 16 16 25 21 11 15 14 oTotal taxa with sensitivity values - - - - - - - - - - - - 19 17 18 15 13 14 22 20 8 13 13 Shannon -Wiener Index - - - - - - - - - - - - 1.77 1.52 2.19 0.94 0.83 2.20 2.19 2.08 1.65 1.04 1.54 E Bla - - - - - - - - - - - - 1.91 1.55 1.87 1.27 1.32 2.25 1.73 1.74 1.31 1.77 1.35 III-G-99 Table III-G5 (concluded). In the ponar summary table below, bold values denote the highest value in a given row (16 bold values total) and bold italics denote the highest value on an upstream or downstream basis (eight bold italic values total- four upstream and four downstream). Ranges AllCreeks(up) 1998 1999 2000 2001 2002 2003 2004 2005 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021 Total abundance Totaltaxa Shannon -Weiner ponar grabs EBI ponar grabs 215 - 1,191 149 - 515 46 - 1,121 51 - 1,356 75 - 295 114 - 615 46 -549 46463 -616 119 - 291 35 - 200 147 - 191 67 -150 166 - 1,865 155 - 2,136 160 - 1,865 I 49 - 2,192 97 - 1,163 59 - 1,422 252 - 1,335 198 - 1,297 123 - 796 50-1,535 104-730 17-21 1.77 - 2.14 1.85 - 2.09 8-17 9-19 0.72 - 1.77 1 0.49 - 2.27 6-24 11-13 8-17 11-30 I 18-20 C 2.01 11-18 9-13 15-19 3-13 12-22 5-22 10-23 8-25 1.14 - 2.49 1.23 - 2.47 12-23 12-23 11-25 8-32 7-22 5-21 8-20 1.47 - 2.41 1.26 - 1.57 0.97 -1.54 1.93 - 2.22 1.48 - 1.56 1.59 - 1.86 1.61 - 2.37 0.32 - 2.04 1.21 - 2.33 1 0.78 - 2.30 1 1.20 - 2.52 1.23 - 2.42 1.17 - 2.39 1.61 - 2.56 0.88 - 2.42 0.99-2.48 0.43-2.27 1.03-1.82 1.98 - 2.03 1.16 - 1.57 1.51 - 1.72 1.67 - 1.73 1.33 - 1.48 1.32 - 1.80 1.58 - 1.69 1.14 - 1.63 1.12 1.32 1.34 - 1.57 1.11 - 1.36 1.23 - 2.31 1.07 - 2.31 1.05 - 2.87 1.35 - 1.88 1.19 - 2.00 1.49 - 2.10 1.38 - 2.02 1.52 - 2.12 1.04 - 2.03 1.22-2.19 All Creeks (down) I Total abundance Totaltaxa Shannon -Weiner ponar grabs EBI ponar grabs 221-919 263 - 274 17-19 314 - 1,291 11-23 398 - 1,088 400 - 1,246 18 442 - 1,639 12-15 _ 326 - 719 21-22 371 - 1,013 196 - 646 91 - 500 220 - 418 139 - 511 216 - 1,329 11-24 123 - 1,415 162 - 2,023 174 - 1,575 161 - 2,018 7-27 147 - 970 207 - 2,641 14-27 351 - 1,548 108 - 953 61-2,105 8-25 37-2,040 8-22 0.38-2.13 1.06-1.96 15-20 16-25 16-18 15-19 12-13 11-26 17-18 10-19 14-28 11-28 14-23 14-25 8-18 1.25 - 2.27 2.07 - 2.45 1.08 - 1.80 1.75 - 2.40 1.71 - 1.95 1.64 - 2.03 1.57 - 1.87 1.03 - 1.55 1.39 - 1.56 2.18 - 2.54 1.59 - 1.81 1.53 - 1.88 1.76 - 1.88 1.39 - 2.52 1.44 - 2.16 1.23 - 2.39 I 1.15 - 2.56 1.31 - 2.46 0.94 - 2.51 0.79 - 2.74 1.45 - 2.52 1.52 - 2.52 1.25 - 2.66 0.72 - 2.13 1.04-2.38 1.74-2.44 1.40 - 1.45 1.62 - 2.13 1.19 - 1.81 1.64 - 1.71 1.24 - 1.29 1.47 - 1.56 1.29 - 2.01 1.13 - 1.45 1.59 - 2.23 1.46 - 1.98 1.11 - 2.39 1.26 - 2.82 I 1.27 - 2.03 11.20 - 3.08 I 1.53 - 2.33 1.53 - 2.10 1.65 - 2.15 1.02 - 1.95 Averages All Creeks (up) I Total abundance Totaltaxa Shannon -Weiner ponar grabs EBI ponar grabs 627 19 1.98 2.00 310 543 661 17 1.42 1.62 185 12 1.42 1.70 365 13 1.26 1.41 298 540 205 15 1.52 1.39 118 11 1.73 1.22 169 17 1.99 1.46 118 736 848 13 1.61 1.66 839 853 18 18 1.84 1.99 1.80 1.60 492 17 1.89 1.73 606 17 1.95 1.86 705 586 18 1.95 1.76 440 15 1.76 1.47 328 13.00 1.79 1.86 316 13 1.48 1.36 13 12 21 19 8 18 18 1.38 1.35 2.08 2.01 1.13 1.99 1.97 1.86 2.00 1.37 1.56 1.64 1.25 1.77 All Creeks (down) Total abundance Totaltaxa Shannon -Weiner ponar grabs EBI ponar grabs 496 18 1.73 1.42 270 746 636 23 2.10 1.79 823 18 1,041 14 1.29 1.48 523 692 421 17 296 13 1.82 1.65 319 19 1.96 1.29 321 808 618 15 1.58 1.75 847 584 20 17 1.93 1.69 1.73 1.51 948 19 1.75 1.62 422 880 18 21 1.92 2.05 1.96 1.87 862 506 14 1.24 1.33 482 16 1.76 1.91 541 13 1.10 1.33 18 15 22 17 17 19 20 2.31 1.38 1.84 2.36 1.70 1.71 1.80 1.96 2.07 1.91 1.38 1.72 1.68 1.27 1.52 1.82 1.78 1.85 III-G-100 Table III-G6. Highest Shannon -Weiner diversity score and highest EBI score by creek per year, highest Shannon -Weiner diversity score and highest EBI score for each year among all creeks, and summary of ranges and averages by creek. Short bold underline separates pre -/post -Mod Alt L years for each impact creek. Pink highlighted cells indicate average scores which were higher post -Mod Alt L; yellow highlighted cells indicate averages where post -Mod Alt L score was lower; grey highlighted cells indicate averages that did not change pre- to post -Mod Alt L. Highest Shannon -Wiener Diversity Score among all Ponars in each Creek by Year ' = not monitored; (u) = upstream (d) = downstream; bold values = highest score all creeks that year Highest EBI Score among all Sweeps in each Creek by Year -' = not monitored; (u) = upstream (d) = downstream; bold values = highest score all creeks that year Year Broomfield Swamp SCUT1 Jacks Jacobs PA2 Drinkwater Little Long Tooley Muddy Huddles Cut Porter DCUT11 DCUT19 Duck Year Broomfield Swamp SCUT1 Jacks Jacobs PA2 Drinkwater Little Long Tooley Muddy Huddles Cut Porter DCUT11 DCUT19 Duck 1998 - - 2.27 (d) - - - - - - - - - - - - 2.04 (u) 2.05 (u) - - - - - - - - - - - - - - - 1998 - - 1.78 (u) - - - - - 1.92 (u) 1.78 (d) - - - - - 1999 - - 2.07 (d) - - - 2.31 (d) 2.45 (d) 2.40 (d) - - 1999 - - 2.05 (d) - - - - - 2.02 (u) 2.00 (d) 2.15 (d) - - - - 2000 - - 1.82 (u) - - - 1.37 (d) 1.08 (d) 2.27 (u) - - 2000 - - 1.77 (d) - - - - - 1.90 (d) 1.94 (d) 2.20 (d) - - - - 2001 - - 1.94 (d) - - - - - - - - 1.81 (u) 2.32 (d) 2.41 (u) - - - - 2001 - - 1.86 (u) - - - - - 2.08 (u) 1.86 (u) 1.94 (u) - - - - 2002 - - 1.64 (d) 1.55 (d) - - - 2.03 (d) 1.54 (u) 2.54 (d) 2.01 (u) 1.88 (d) 1.86 (u) 2.52 (d) 1.81 (d) - - 2002 - - 1.93 (d) - - - - - - 2.42 (d) - - - - - 2003 - - - - - - - 2003 - - 2.04 (d) - - - - - - 1.88 (d) - - - - - 2004 - - 2.22 (u) - - - - - - - - - - 2004 - - 2.19 (d) - - - - - - 2.05 (d) - - - - - 2005 - - 2.01 (u) - - - - - - - - - - - 2005 - - 2.05 (d) - - - - - - 2.01 (u) - - - - - 2007 - - - - - - - - - 1.56 (u) - - - - 2007 - - - - - - - - - 1.95 (d) 2.07 (u) - - - - 2008 - - - - - - - - - 1.88 (d) - - - - 2008 - - - - - - - - - 2.48 (d) 2.11 (u) - - - - 2009 - - - - - - - - - 1.61 (u) - - - - 2009 - - - - - - - - - 1.99 (d) 2.22 (u) - - - - 2010 - - - - - - - - 2.16 (d) 1.44 (d) - - - - 2010 - - - - - - - - 1.96 (d) 1.98 (u) 2.19 (d) - - - - 2011 - - 2.39 (d) 2.10 (u/d) 2.31 (d) 2.33 (d) 2.01 (d) 2.04 (u) 2.30 (u) 2.17 (d) 2.09 (d) 2.14 (u) - - 2.20 (u) 2011 - - 2.03 (d) 2.10 (d) 2.22 (d) 2.18 (u) 1.99 (d) 2.05 (u) 1.99 (u) 1.98 (d) 2.26 (d) 2.07 (d) - - 1.90 (d) 2012 - - 1.24 (d) 1.92 (u) 1.15 (d) 2.08 (u) 1.65 (u) 1.49 (u) 1.57 (d) 2.08 (d) 2.56 (d) 2.30 (u) - - 1.93 (u) 2012 - - 1.99 (u) 2.40 (u) 2.33 (d) 1.96 (u) 2.02 (d) 1.98 (d) 2.08 (u) 2.15 (d) 2.26 (d) 2.17 (d) - - 2.02 (d) 2013 - - 1.62 (u) 1.98 (u) 1.84 (d) 2.46 (d) 2.34 (d) 1.71 (d) 1.86 (u) 2.22 (d) 2.43 (d) 1.87 (d) 1.83 (u) 2.49 (u) 2.19 (d) 2013 - - 2.07 (u) 2.07 (u) 1.98 (u) 1.93 (u) 2.25 (d) 1.97 (d) 1.90 (d) 1.88 (d) 2.19 (d) 2.05 (u) 2.03 (d) 1.90 (d) 1.92 (u) 2014 - - 2.51 (d) 1.89 (u) 1.71 (d) 2.32 (u) 1.94 (u) 1.57 (u) 2.43 (u) 1.23 (u) 2.47 (u) 2.01 (u) 2.19 (d) 2.01 (d) 2.09 (u) 2014 - - 1.86 (d) 1.86 (d) 1.77 (d) 1.88 (d) 1.84 (d) 2.03 (d) 2.07 (d) 1.87 (d) 2.01 (u) 2.01 (d) 1.86 (d) 1.97 (d) 1.97 (d) 2015 - - 2.20 (u) 2.14 (u) 2.35 (d) 2.27 (u) 2.40 (d) 1.91 (u) 2.25 (u) 1.58 (d) 2.52 (u) 1.53 (u) 1.88 (d) 2.74 (d) 2.17 (u) 2015 - - 2.01 (d) 2.01 (d) 1.84 (d) 1.98 (u) 2.05 (d) 1.97 (d) 1.94 (d) 1.93 (d) 2.11 (d) 2.03 (d) 1.80 (d) 1.87 (u) 1.96 (d) 2016 - - 2.00 (u) 2.42 (u) 2.16 (d) 1.87 (u) 2.08 (d) 2.12 (d) 2.07 (u) 1.89 (u) 2.52 (d) 2.00 (d) 2.12 (u) 2.11 (u) 2.20 (d) 2016 - - 2.00 (d) 1.98 (d) 1.76 (d) 1.88 (d) 1.80 (d) 2.12 (d) 2.03 (d) 2.09 (d) 2.17 (d) 2.06 (d) 1.86 (d) 1.77 (u) 2.13 (d) 2017 - - 2.22 (u) 2.15 (u) 1.88 (d) 2.00 (d) 2.24 (d) 2.27 (u) 2.39 (u) 2.19 (u) 1.93 (u) 2.03 (d) 1.52 (d) 2.52 (d) 2.19 (d) 2017 - - 2.04 (d) 1.80 (d) 1.81 (u) 1.94 (u) 1.79 (d) 1.99 (d) 1.98 (d) 1.87 (d) 2.07 (d) 2.07 (d) 1.98 (d) 1.87 (d) 2.08 (d) 2018 - - 1.89 (d) 2.09 (u) 2.46 (d) 2.43 (d) 2.07 (d) 2.66 (d) 2.21 (u/d) 2.31 (d) 2.30 (u) 2.00 (d) 1.78 (d) 1.67 (u) 2.08 (d) 2018 - - 2.02 (d) 1.91 (d) 1.85 (u) 1.87 (u) 2.07 (d) 2.01 (d) 2.01 (d) 1.95 (d) 2.11 (d) 2.08 (d) 2.03 (d) 2.00 (u) 1.89 (d) 2019 1.75 (u) 2.13 (d) 2.17 (u) 1.92 (u) 1.48 (u) 1.97 (u) 1.95 (u) 1.11 (u) 2.11 (u) 1.02 (d) 1.51 (d) 2.08 (u) 2.42 (u) 1.78 (u) 1.94 (u) 2019 1.46 (u) 1.72 (d) 1.74 (d) 2.24 (u) 1.83 (d) 1.98 (u) 2.04 (d) 1.91 (d) 1.91(u) 1.95 (d) 2.18 (d) 2.00 (d) 1.65 (d) 1.81 (d) 2.01 (d) 2020 1.21 (u) 1.74 (d) 1.99 (u) 2.48 (u) 2.17 (u) 2.38 (d) 2.27 (d) 1.84 (u) 2.31 (u) 2.25 (d) 2.01 (d) 1.76 (u) 2.07 (u) 2.00 (u) 1.34 (u) 2020 1.64 (d) 1.98 (d) 1.93 (d) 1.98 (d) 1.86 (u) 1.82 (u) 1.83 (d) 2.09 (d) 2.01 (u) 2.02 (u) 2.36 (d) 2.01 (u) 2.07 (u) 1.93 (d) 2.07 (d) 2021 2.02 (d) 2.02 (u) 1.67 (u) 1.45 (u) 2.11 (d) 1.78 (u) 1.32 (u) 0.54 (d) 1.22 (u) 0.71 (u) 1.26 (d) 1.68 (u) 2.04 (u) 2.13 (d) 2.27 (u) 2021 1.48 (d) 1.83 (d) 1.98 (d) 1.92 (d) 1.75 (d) 1.82 (u) 2.04 (d) 1.86 (d) 1.95 (u) 2.01 (d) 1.93 (d) 2.06 (d) 1.52 (u) 1.88 (d) 2.03 (d) Total range (u) 1.21-2.02 1.62-2.22 1.45-2.48 1.48-2.17 1.78-2.32 1.32-1.95 1.11-2.27 1.22-2.43 0.71-2.19 1.56-2.52 1.53-2.30 1.83-2.42 1.67-2.49 1.93-2.27 Total range (u) 1.46/- - 1.78-2.07 2.07-2.40 1.81-1.98 1.82-2.18 - 2.05 1.91-2.08 1.86-2.02 1.94-2.22 2.01-2.05 1.52/2.07 1.77-2.00 1.92 Total avg (u) 1.48 2.02 1.99 2.05 1.83 2.05 1.72 1.75 2.08 1.69 2.10 1.93 2.10 2.01 1.99 Total avg (u) 1.46 - 1.93 2.24 1.88 1.94 - 2.05 2.01 1.97 2.07 2.03 1.80 1.82 1.92 Avg pre/post (u) 1.48/- 1.92/2.04 2.00/2.07 - 2.08/2.04 - - - MI:31 2.00/1.84 1.98/2.18 - - Avg pre/post (u) 1.46/- - 1.93/- - 07/1. - - - `2.09/2.01 -/1.80 - - Total range (d) 2.02/- 1.74-2.13 1.24-2.51 2.10 1.15-2.46 2.00-2.46 2.01-2.40 0.54-2.66 1.37-2.31 1.02-2.54 1.26-2.56 1.87-2.03 1.52-2.19 2.01-2.74 2.08-2.20 Total range (d) 1.48/-64 1.72-1.98 1.74-2.05 1.80-2.10 1.75-2.33 1.88/'- 1.79-2.25 1.86-2.12 1.90-2.07 1.78-2.48 1.93-2.36 2.00-2.17 1.65-2.03 1.81-1.97 1.89-2.13 Total avg (d) 2.02 1.94 1.94 2.10 2.00 2.32 2.20 1.76 1.92 2.0 2.01 1.98 1.84 2.35 2.17 Total avg (d) 1.56 1.84 1.98 1.95 1.93 1.88 1.97 1.99 1.97 2.01 2.17 2.06 1.89 1.89 2.01 Avg pre/post (d) 2.02/- 1.94/- 2.101- - - - I1.95/1U - 1.87/2.01 1.86/11 - - Avg pre/post (d) 1.48/- - 1.99/1.96 2.10/1.92 - -/1.88 - - 1.93/11-- 2.18/2.17 2.07/2.05 1.91/1.84 - - Highest EBI Score among all Ponars in each Creek by Year = not monitored; (u) = upstream (d) = downstream; bold values = highest score all creeks that year Summary of Highest Shannon Weiner Diversityand EBI Scores Year Broomfield SCUT1 Jacks Jacobs PA2 Drinkwater Little Long Tooley Muddy Huddles Porter DCUT11 DCUT19 Duck Swamp Cut 1998 - - 1.85 (u) - - - - - 2.09 (u) 2.05 (u) - - - - - 1999 2.13 (d) 2.07 (d) 2.00 (u) 1.98 (u) 2000 - - 1.57 (u) 1.22 (d) 1.28 (d) 1.81 (d) 2001 - - 1.95 (d) 1.77 (d) 1.72 (d) 1.72 (u) 2002 - - 1.67 (u) 1.87 (d) 2003 - - 1.48 (u) 1.39 (d) 2004 - - 1.80 (u) 1.64 (d) 2005 - - 1.58 (u) 2007 1.69 (u) 1.24 (d) 1.63 (u) 2008 2.01 (d) 1.29 (d) 2009 - - - - - - - - - 2.52 (d) 1.34 (u) - - - - 2010 - - - - - - - - 1.59 (d) 1.63 (d) 2.23 (d) - - - - 2011 - - 1.57 (u) 1.66 (u) 1.89 (u) 1.88 (u) 1.98 (u) 2.31 (u) 1.89 (d) 1.63 (d) 1.96 (d) 1.94 (d) - - 1.91 (d) 2012 - - 1.11 (d) 2.22 (u) 2.39 (d) 2.22 (d) 1.84 (d) 1.72 (d) 1.42 (d) 1.65 (u) 1.66 (d) 1.84 (u) - - 1.73 (u) 2013 - - 1.41 (u/d) 2.25 (u) 2.87 (u) 1.86 (d) 1.71 (u/d) 1.89 (u) 1.80 (u) 1.74 (d) 1.49 (d) 1.91 (u) 2.07 (d) 1.78 (d) 1.87 (d) 2014 - - 1.63 (u) 1.47 (d) 1.75 (u) 1.68 (d) 1.45 (u) 1.49 (u) 1.54 (d) 1.41 (u) 2.03 (d) 1.80 (u) 1.87 (u) 1.73 (u) 1.54 (u) 2015 - - 1.84 (u) 2.00 (u) 1.89 (d) 1.71 (u) 1.75 (d) 1.45 (u) 1.73 (u) 1.31 (d) 3.08 (d) 1.96 (u) 1.88 (u) 1.88 (u) 1.86 (u) 2016 - - 2.33 (d) 2.27 (d) 2.06 (d) 2.23 (d) 1.88 (u) 2.05 (u) 2.03 (u) 1.53 (d) 1.84 (d) 1.90 (u) 2.02 (d) 2.03 (d) 2.25 (d) 2017 2.10 (d) 2.02 (d) 2.02 (u) 2.03 (d) 1.90 (d) 2.03 (d) 2.00 (u) 2.02 (u/d) 1.53 (d) 1.91 (u) 2.00 (u) 1.95 (u) 1.83 (u) 2018 - - 2.06 (d) 1.71 (u) 1.65 (d) 1.68 (u) 1.72 (d) 1.85 (d) 1.79 (d) 1.91 (d) 2.15 (d) 1.89 (d) 2.05 (d) 2.12 (u) 1.95 (u) 2019 1.04 (u) 2.13 (d) 1.43 (u) 1.40 (u) 1.42 (u) 1.76 (u) 1.49 (u) 1.28 (d) 1.51 (u) 1.24 (d) 1.49 (d) 1.68 (u) 1.95 (d) 2.03 (u) 1.64 (u) 2020 1.91 (d) 1.77 (d) 1.93 (d) 2.02 (d) 2.19 (u) 1.84 (d) 2.00 (u) 2.04 (u) 1.86 (u) 1.94 (u/d) 2.44 (d) 2.06 (u) 1.99 (u) 2.00 (u) 2.01 (u) 2021 1.20 (d) 1.96 (d) 1.20 (d) 1.13 (u) 1.47 (d) 1.74 (u) 1.49 (u) 1.18 (u) 1.25 (u) 1.20 (u) 1.55 (d) 1.73 (u) 1.82 (u) 1.71 (d) 1.64 (u) Total range (u) 1.04/'- 1.41-2.13 1.13-2.25 1.42-2.87 1.68-1.88 1.45-2.00 1.18-2.31 1.25-2.09 1.20-2.05 1.34-1.98 1.68-2.06 1.82-2.00 1.73-2.12 1.54-2.01 Total avg (u) 1.04 - 1.62 1.77 2.02 1.75 1.71 1.77 1.78 1.75 1.67 1.87 1.91 1.95 1.78 Avg pre/post (u) 1.04/- - 1.62/1.64 2.04/1.56 - 1.88/1.ii - 1.67/- 1.88/1. 1.92/1 . - Total range (d) 1.20-1.91 1.77-2.13 1.11-2.33 1.47-2.27 1.47-2.39 1.68-2.23 1.71-1.90 1.28-2.03 1.22-2.07 1.24-2.52 1.29-3.08 1.89-1.94 1.95-2.07 1.71-2.03 1.87-2.25 Total avg (d) 1.56 1.95 1.80 1.95 1.89 1.98 1.78 1.72 1.66 1.68 1.90 1.92 2.02 1.84 2.01 Avg pre/post (d) 1.56/1.91 1.65/1.92 -/1.95 - r2.22/1.93 1.71/1.58 - r1.55/1.95 1.94/1.89 2.04/2.00 Shannon -Wiener Diversity Ponar Grabs: years w/ upstream highest score years w/ downstream highest score 8 of 23 or 34.8% 16 of 23 or 69.6% Downstream Average Scores Compared to Upstream Average Scores: EBI Ponar Grabs: years w/ upstream highest score years w/ downstream highest score EBI Sweeps: years w/ upstream highest score years w/ downstream highest score Shannon -Wiener Diversity Ponar Grabs: 10 of 15 creeks had higher downstream averages EBI Ponar Grabs: 10 of 15 creeks had higher downstream averages EBI Sweeps: 11 of 15 creeks had higher downstream averages Creeks with Highest Percent of Monitored Years with Highest Scores: Shannon -Wiener Diversity Ponar Grabs: Huddles Cut 27.8% (5 of 18 years; 2 of which are post all drainage basin reduction) Jacks 26.3% (5 of 19 years, which none are post all drainage basin reduction) Tooley 6.3% (1 of 16; which none are post all drainage basin reduction) Muddy 26.1% (6 of 23 years) EBI ponar grabs: Huddles Cut 38.9% (7 of 18 years; 5 of which are post all drainage basin reduction) Jacks 31.6% (6 of 19 years; 2 of which are post all drainage basin reduction) Tooley 6.3% (1 of 16; which none are post all drainage basin reduction) Muddy 17.4% (4 of 23 years) EBI sweeps: Huddles Cut 61.1% (11 of 18 years; 7 of which are post all drainage basin reduction) Jacks 15.8% (3 of 19; which none are post all drainage basin reduction) Tooley 18.8% (3 of 16; 1 of which is post all drainage basin reduction) Muddy' 8.7% (2 of 23) 7 of 23 or 30.4% 16 of 23 or 69.6% 5 of 23 or 21.7% 18 of 23 or 78.3% III-G-101