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HomeMy WebLinkAbout20080868 Ver 2_Appendix I Benthos_20220605APPENDIX! BENTHOS Abundance, Taxa richness, Estuarine Biotic Index [EBI] and Hydrographic Data Collected in 2021 2021 Sweeps Tables 1-1 to 1-32 Summary all creeks and by each creek 2021 Ponars Tables 1-33 to 1-94 Summary all creeks and by each creek 2021 Hydrographic Data Tables 1-95 to 1-109 (Tables only on Flash Drive) Table 1-1. Raw data from benthic sweep samples collected in May 2021 at each station in all 15 creeks in the PCS study. Benthic organisms were not counted beyond 100 individuals in any sample. Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up I Down Annelida Rhynchobdellida Piscicolidae Myzobdella lugubris Clitellata Enchytraeidae sp. Naididae Naididae sp. Naididae sp. w/ hair Dero sp. Naididae sp. w/o hair Limnodrilus hoffineisteri Nais sp. Paranais sp. Paranais litoralis Tubificoides heterochaetus Polychaeta Ampharetidae Amphicteis floridus Nereididae Laeonereis culveri Neanthes succinea Spionidae Boccardiella sp. Polydora cornuta Streblospio benedicti - - - - - - - - - 2 - - 2 - - 2 - - 3 5 - - - 2 - - - - - - 2 4 - - 5 8 4 2 22 1 6 2 2 4 4 2 11 - 8 1 - - 7 - 4 1 9 - - 14 - 6 - - - - - - - - - - - - - - - - - - - - - - - - - - 12 - 3 - - - - - - - - - - - - - - - - - - - - - - - - - - - 5 1 5 - - 1 - - - - - - - - - - - - - - - - - - - - - - - - 38 - 82 33 207 - 5 - 32 5 16 10 - - 6 4 4 - 4 2 6 - - 1 31 - 18 3 7 7 9 4 2 6 33 1 - - 10 - 2 - - - - - - - - - - - - - 6 - - - - - - - - 5 - - - - 2 - - - - - - - - - - - - - - - - - - - - - 6 - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - 1 - 3 - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - 2 1 - - - - - - 8 - - - - - - - - - - 4 - 41 31 1 43 3 15 54 36 49 44 31 108 5 172 41 135 56 27 2 115 1 47 6 31 64 20 4 - 1 - 1 - - 3 - 10 4 - 2 2 4 - - 20 1 1 - - - 2 - 2 - - 2 - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - - 2 - - - - - - - - - - - - - - - - - - - 4 - - - - 2 - - - - - - - - - - - - - - - Arthropoda Arachnida Acari sp. Arachnida sp. Insecta Coleoptera Hydrophilidae Berosus sp. Hydropsychidae Hydropsychidae sp. Dytiscidae Neoporus sp. Haliplidae Haliplus fasciatus Peltodytes sp. Ablabesmyia sp. Noteridae Hydrocanthus oblongus - - - - - - - - - - - - - - - - - - - - - - 3 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 8 - 2 - - - - - 2 14 - - - 25 - 5 8 14 4 - - - - 9 - - - 1 - 36 9 33 3 18 - - - - - - - - - - - - - - - - - 4 - - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - 1 - - - 1 4 2 2 - 1 - 1 - 3 2 6 - - 2 - 4 - - - - - - 2 - - - - - 2 - 1 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - - Table 1-1. (continued). Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up I Down Diptera Ceratopogonidae Bezzia/Palpomyia complex Dasyhelea sp. Chironomidae Chironominae (subfamily) Apedilum sp. Chironomus decorus Chironomus stigmaterus Chironomus sp. Chrysops sp. Cladopelma sp. Cladotanytarsus sp Clinotanypus sp. Cricotopus sp. Cryptochironomussp. Dicrotendipes nervosus Endochironomus sp. Goeldichironomus devineyae Glyptotendipes sp. Kiefferulus sp. Nanocladius sp. Orthocladiinae sp. Parachironomus sp. Polypedilum sp. Polypedilum halterale Polypedilum tritum Procladius sp. Tanypus neopunctipennis Tanytarsus sp. Tribelos jucundus Tribelos sp. Curculionidae sp. Dolichopodidae Dolichopodidae sp. Ephydridae sp. Tabanidae Tabanus sp. Tipulidae Ormosia sp. Tipula sp. Baetidae Baetidae sp. Callibaetis sp. Hemiptera Belostomatidae Abedus/Belostoma sp. Belostoma lutarium - 13 4 - 3 - 1 - 5 3 2 8 6 - - - 4 - - - 2 - 3 - 4 3 4 2 5 - - 4 - - - - 1 - 2 3 - - - - - - - - - - 1 - - - - 3 3 - - 4 - - - - - - - - - - - - - 4 - - - - - - - - - - - - - - - - - - - - - - - - 1 1 2 - - - - - - - - - - - - - - - - - - - 4 10 61 13 47 2 131 - 22 80 140 67 142 101 300 73 59 44 56 65 75 - 11 - - - 10 51 77 8 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 35 - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - 3 - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2 - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 - - 7 - - - 14 3 41 36 - - 10 5 4 - 1 - - 7 4 - 5 60 - - - - 4 - 7 - - - - - - - - - - - - - - - - - - - - - - - - - - - 2 40 6 28 18 3 20 1 1 52 11 57 36 42 14 19 81 44 26 5 - 7 8 1 102 141 52 20 282 71 - - - - 5 - - - - 2 - 3 - - - - - - - - - - 1 - - - - - - - - - - - 44 14 1 3 165 94 252 266 82 155 60 29 64 23 71 24 8 2 40 10 - - 81 27 6 40 - - 4 - 1 - - - - 4 - - - - - - - - - - - - - - - - - - - - 4 - 1 1 - - 4 - - - - - - - - - - - - - - - - - - - - - 3 - - - - 1 - - - - - 2 2 5 4 - - 2 4 5 2 - - - 6 - 1 1 - - 2 5 - 2 - - - - - - - - - 2 - - - - - - - - - - - - - - - - - - - - - 1 5 - - - 3 - 10 16 2 - 4 4 - 10 - 1 - 1 1 2 - - 4 - - - - - - - - - 5 - - - - - - 1 - - - - - - - - 1 - - 6 3 1 - 8 - - 9 4 - - - - - - - - - - - - - - - - - - - - - - - - - - 5 8 9 3 - - - - - - - - - - - - - - - - - - - - - - - - - - 2 - 2 5 - - 5 - - - - - - - - - - - - - 1 - - - - 1 - - - - - 11 21 1 14 - 4 - - - 10 2 - - 28 - 4 - - - 1 - - - - - - 2 14 - 12 70 43 13 15 - 58 - 28 31 19 23 70 69 62 30 300 102 94 14 28 2 6 - 66 39 78 29 173 44 10 - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - 2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - - - - - 1 - 1 - 1 - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - 2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2 - - - - - 2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2 - - - - - - - - - - - - - - - 2 - 1 - - - - - - - - - - - - - - - - - - - - - - - 6 - - - - - 1 - - - 13 2 - - - 2 - - - - - - 3 - - - - - - - 1 1 - - - - - - 1 - - 2 2 - - - - - - - - - 4 - 1 8 2 - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 - - 1 - - 1-2 Table 1-1. (continued). Taxonomic hierarchy Collembola sp. Corixidae Corixidae sp. Sigara sp. Trichocorixa calva Trichocorixa kanza Trichocorixa sexcinta Gerridae Gerridae sp. Rheumatobates sp. Naucoridae Pelocoris sp. Pelocoris carolinensis Pelocoris femoratus Sminthuridae sp. Odonata Corydalidae Chauliodes pectinicornis Coenagrionidae Enallagma sp. Libellulidae Libellulidae sp. Libellula sp. Pachydiplaxlongipennis Perithemis tenera Leptoceridae Oecetis inconspicua Oecetis sp. Nepidae Ranatra nigra Malacostraca Amphipoda Corophiidae Apocorophium lacustre Apocorophium louisianum Apocorophium sp. Gammaridae Gammarustigrinus Haustoriidae Lepidactylus dytiscus Talitridae Uhlorchestia (Orchestia) uhleri Daphniidae Daphnia sp. Decapoda Cambaridae sp. Palaemonidae Palaemonetes pugio Panopeidae Rhithropanopeus harrisii Sesarmidae Sesarma reticulatum Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up I Down 1 - - - - - - - - - - - - - - - - - - - - - - - - - - 108 30 164 1 4 1 9 - - - 43 55 2 - 44 2 - 4 4 4 - - 11 - 2 1 - - 23 - - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 6 9 1 - - 3 - - - - - - - - - - - - - - - - - - - - - - - 2 6 - - - - - - - - 2 - - - - - - - - - 1 - - - - - - - - - - - - - - - - - - 1 5 14 - - 4 - - - - 1 - - 2 - - - - - - - - - - 1 - - - - - - - - - - - - - - - - -- - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2 - - - - - - 1 - - - - - - - - - - - - - - - - - - 1 - - - - - - - - - - 6 - - - - - - - 2 1 - - 4 - - - - - - - 2 2 - - - 9 - - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - 3 1 3 - 4 - - - 2 1 4 12 4 - 2 - - 4 - 1 - - 5 - 2 3 2 - - - - - - - - - - - - - - - 2 - - - - - - - - - - - - - - - - - - - - - - - - - - - 3 2 - - - - - - - - - - - - 1 - - - 3 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 3 - - 1 1 - 1 - 2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2 - - 8 - 2 - - - - - - - - - - - 2 - - - 1 2 - - - - 4 - 4 - - 2 - - - - 6 - - - - 23 - - - 15 5 10 16 50 8 36 4 124 3 71 11 6 1 9 - 2 - 4 - - - 1 - - - 251 3 - 1 79 40 140 83 258 11 158 35 260 87 243 160 24 8 25 4 1 - 2 - - 4 4 89 2 300 123 36 46 6 2 121 54 86 260 127 26 105 204 101 251 191 3 90 263 22 21 15 32 2 300 151 - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - 1 - - - 1 - - - - - - - - - - - - - - - - 1 - 2 - - - - - - 22 - - - - - 3 - - - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 13 16 - 15 59 34 16 8 22 13 34 12 18 52 58 88 8 7 17 24 - - 17 81 - 156 - - - - - - - 2 - 5 - - - 1 2 1 - - - - 4 2 - 1 - - - - - 4 - - - - - - - - - - - - - - - - - - - - - - - 1 - - - - - - 1-3 Table 1-1. (concluded). Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up I Down Isopoda Anthuridae Cyathura polita Bopyroidea Probopyrus pandalicola Idoteidae Edotia montosa Sphaeromatidae Cassidinidea ovalis Mysida Mysidae Americamysis almyra Ostracoda Candonidae Candonidae sp. Cytherideidae Cyprideis littoralis Tanaidacea Leptocheliidae Hageria rapax Maxillopoda Sessila Balanidae Balanus subalbidus Balanus improvisus Mollusca Bivalvia sp. Dreissenidae Mytilopsis leucophaeata Mytilidae Geukensia demissa Gastropoda Basommatophora Planorbidae sp. Physidae sp. (physalphysella) Hydrobiidae Littoridinops sp. - - - 1 - 1 - 1 - 2 - - 4 2 - - 35 3 2 - - - - - 2 2 2 2 - 4 - - - - - 2 - - - - - - - 2 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2 1 - 1 3 13 - - - 1 - - - - - - - - - 19 1 22 7 1 15 - 6 2 4 12 36 1 16 1 3 - 7 7 - - 20 9 39 35 21 16 - 17 - - - - 4 7 - - - 20 - - 8 42 88 92 - 34 1 9 - - - - - - - - - 1 - - - - - - - - - - - - - - 4 - - - 1 - - - - - - - 2 - - 8 - 56 47 158 25 1 239 9 30 7 9 13 - 12 58 1 110 1 82 9 300 - 33 1 9 8 3 2 34 4 - - - - - 5 - - - 135 - 6 - 180 - 116 288 120 - 2 - 2 - - - - - 2 - 4 - - - - - 2 - 5 - - - - - - - 30 - 2 - 1 - - - 25 - - 1 - - 5 - - - - - - - - - 4 - - - - - - - - - - - - - - - - - - - - - - - - - 1 - - 1 3 2 5 4 8 2 - - - 3 1 - - - - 2 1 2 - - - 11 32 - 5 2 - 2 1 - - - - - - - - - - - - - - 1 - 1 - - - 134 - - - - - - - - - - - - - - - - - 3 - - 1 - - - - - - - - - - - - - - - - 1 - - - - - - - - - - - - - - - - - - - - - - - 6 3 6 - - - 7 - - - - - - - - - - - - - - - - - - - - - - - 1 7 2 23 257 204 16 13 300 175 294 300 220 167 300 98 300 200 275 259 23 3 244 89 95 59 300 156 227 40 Totaltaxa 26 29 28 28 31 19 29 16 27 32 27 30 28 27 24 26 24 22 24 23 17 15 33 16 29 24 24 23 19 24 — = Species not collected at station 1-4 Table 1-2. Taxa collected in May 2021 from each benthic sweep sample station in all 15 creeks in the PCS study. Taxa for which EBI was calculated are shown with a * and other taxa are shown with a V. Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) control Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek control ( ) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Annelida Rhynchobdellida Piscicolidae Myzobdella lugubris - - - - - - - - - ✓ - - ✓ - - ✓ - - ✓ ✓ - - - ✓ - - - - - - Clitellata Enchytraeidae sp. * * - - * * * * * * * * * * * * * - * * - - * - * * * - - * Naididae Naididae sp. - * - - - - - - - - - - - - - - - - - - - - - - - - - * - Naididae sp. w/ hair * - - - - - - - - - - - - - - - - - - - - - - - - - - - * * Dero sp. * - - * - - - - - - - - - - - - - - - - - - - - - - - - * Naididae sp. w/o hair * * * - * - * * * * - - * * * - * * * - - * * - * * * * * * Limnodrilus hoffmeisteri * * * * - - * - * - - - - - - - - - - - - - * - - - - - - - Nais sp. - * - - - - * - - - - - - - - - - - - - - - - - - - - - * - Paranais sp. - - - - - - - - - - - - - * - - - - - - - - - - - - - - - - Paranais litoralis - * - * - - - - - - - - - - - * - - - - - - - - - - - - - - Tubificoides heterochaetus - - - - - - - - - - - - * * - - - - - - * - - - - - - - - - Polychaeta Ampharetidae Amphicteis fioridus - * - * * * * * * * * * * * * * * * * * * * * * * * * * * * Nereididae Laeonereis culveri - * - * - - * - * * - * * * - - * * * - - - * - * - - * - - Neanthes succinea - - - - - - - - - - - - - - - - - - - - * - - - - - - - - - Spionidae Boccardiella sp. - - - - - - - - - - - - - - - - - - - - - - - - ✓ - - - - - Polydora cornuta - - - - - - - - - - - - - - - - - - - * - - - - - - - - - - Streblospio benedicti - - - - - - - - - * - - - - * - - - - - - - - - - - - - - - Arthropoda Arachnida Acari sp. - - - - - - - - - - - - - - - - - - - - - - ✓ - - - - - - - Arachnida sp. - - - - - - - - - - - - - - - - - - - - - - - - - * - * - - Insecta Coleoptera Hydrophilidae Berosus sp. - - - * * - - - * - * * * * - - - - * - - - * - * * * * * - Hydropsychidae Hydropsychidae sp. - - - - - - - - - - - - - - - - ✓ - - - - - - - - - - - - - Dytiscidae Neoporus sp. - - V - - - - - - - - - - - - - - - - - - - - - - - - - - - Haliplidae Haliplus fasciatus - - - - - - - - - ✓ - - - - - - - - - - - - - - - - - ✓ - - Peltodytes sp. - * * * * - * - * - * * * - - * - * - - - - - - * - - - - - Ablabesmyia sp. ✓ - ✓ ✓ - - - - - - - - - - - - - - - - - - - - - - - - - - Noteridae Hydrocanthus oblongus - - - - - - - - - - - - - - - - - - - - - - ✓ - - - - - - - 1-5 Table 1-2 (continued). Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Diptera Ceratopogonidae Bezzia/Palpomyia complex - * * - * - * - * * * * * - - - * - - - * - * - * * * * * - Dasyhelea sp. - V - - - - V - V V - - - - - - - - - - V - - - - V V - - V Chironomidae Chironominae (subfamily) - - - - - - - - - - - - - V - - - - - - - - - - - - - - - - Apedilum sp. - - - - - - - - * * * - - - - - - - - - - - - - - - - - - - Chironomus decorus * * * * * * * - * * * * * * * * * * * * * - * - - - * * * * Chironomus stigmaterus - - - - - - - - - - - - - - - - - - - - - - - - - - - V - Chironomus sp. - - - - - - - - - - - - - - - - - - - - - * - - - - - - - - Chrysops sp. - - - - - - - - * - - - - - - - - - - - - - - - - - - - - - Cladopelma sp. - V - - - - - - - - - - - - - - - - - - - - - - - - - - - - Cladotanytarsus sp - - - - * - - - - - - - - - - - - - - - - - - - - - - - - - Clinotanypus sp. - - * - - - - - - - - - - - - - - - - - - - - - - - - - - - Cricotopus sp. - * - - * - - - * * * * - - * * * - * - - * * - * * - - - - Cryptochironomus sp. * - * - - - - - - - - - - - - - - - - - - - - - - - - - - - Dicrotendipes nervosus * * * * * * * * * * * * * * * * * * * * - * * * * * * * * * Endochironomus sp. - - - - * - - - * - * - - - - - - - - - - * - - - - - - - Goeldichironomus devineyae - - - - * * * * * * * * * * * * * * * * * * * * - - * * * * Glyptotendipes sp. - - * - * - - - - * - - - - - - - - - - - - - - - - - - - - Kiefferulus sp. V - V V - - V - - - - - - - - - - - - - - - - - - - - - V - Nanocladius sp. - - - * - - - - - * * * * - - * * * * - - - * - * * - - * * Orthocladiinae sp. - V - - - - - - - - - V - - - - - - - - - - - - - - - - - - Parachironomus sp. - - - * * - - - * - * * * - * * - * - * - * * * - - * - - - Polypedilum sp. - - - - - - * - - - - - - * - - - - - - - - * - - * * * - * Polypedilum halterale - - V V - - - - - - - - - - - - - - - - - - - - - - - - - - Polypedilum tritum * V * * - - - - - - - - - - - - - - - - - - - - - - - - - - Procladius sp. * - * * - - * - - - - - - - - - - - - - * - - - - * - - - - Tanypus neopunctipennis - * * * * - * - - - * * - - * - * - - - * - - - - - - * * - Tanytarsus sp. * * * * * - * - * * * * * * * * * * * * * * * - * * * * * * Tribelos jucundus V - V - - - - - - - - - - - - - - - - - - - - - - - - - - - Tribelos sp. V - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Curculionidae sp. - - - - - - - - - - - - - - - - - - - - - - - ✓ - ✓ - - - - Dolichopodidae Dolichopodidae sp. - - - - * - * - * - - - - - - - - - - - - - * - - - - - - - Ephydridae sp. - - - - - - - V - - - - - - - - - - - - - - - - - - Tabanidae Tabanussp. - - - - - - - - - - - - - - - - - - - - - - - - * - - - - - Tipulidae - - - Ormosia sp. V - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Tipula sp. - - - - - - - - - - - - - - - V - - - - - - - - - - - Baetidae Baetidae sp. - - - - V - V - - - - - - - - - - - - - - - - - - - - - - - Callibaetis sp. * - - - - - * - - - * * - - - * - - - - - - * - - - - - - - Hemiptera Belostomatidae Abedus/Belostoma sp. * * - - - - - - * - - * * - - - - - - - - - * - * * * - - - Belostoma lutarium - - - - - - - - - - - - - - - - - - - - - - - - V - - V - - 1-6 Table 1-2 (continued). Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Collembola sp. - - - V - - - - - - - - - - - - - - - - - - - - - - - - - - Corixidae Corixidae sp. * * * * * * * - - - * * * - * * - * * * - - * - * * - - * - Sigara sp. - * - - - - - - - - - - - - - - - - - - - - - - - - - - - - Trichocorixa calve - V V V - - V - - - - - - - - - - - - - - - - - - - - - - - Trichocorixa kanza V V - - - - - - - - V - - - - - - - - - V - - - - - - - - - Trichocorixa sexcintca - - - - - - - - - * * * - - * - - - - * - - * - - - - - - - Gerridae Gerridae sp. - - - - - - - ✓ - - - - - - - - - - - - - - - - - - - - - - Rheumatobates sp. V - - - - - - - - - - - - - - - - - - - - - - - - - - - Naucoridae Pelocoris sp. - - - V - - - - - - - - - - - - - - - - - - - - - - - - - - Pelocoris carolinensis - - - - - - - - - - - - - - - - - - - - - - - - - - - * - - Pelocoris femoratus - - - - * - - - - - - - - - - - - - - - - - - * - - - - - - Sminthuridae sp. - - - - V - - - - - - - V V - - V - - - - - - - V V - - - V Odonata Corydalidae Chauliodes pectinicornis - - V - - - - - - - - - - - - - - - - - - - - - - - - - - - Coenagrionidae Enallagma sp. * * * - * - - - * * * * * - * - - * - * - - * - * * * - - - Libellulidae Libellulidae sp. - - - - - - - - - - - - ✓ - - - - - - - - - - - - - - - - - Libellula sp. - - - - - - - - - - * * - - - - - - - - - - - - * - - - * - Pachydiplax longipennis - - - - - - - - - - - - - - - - - - - - - - - - - - - - * - Perithemis tenera - V V - V - V - - - - - - - - - - - - - - - - - - - - - - - Leptoceridae Oecetis inconspicua - - - - - - - - - - - - - - - * - - - - - - - - - - - - - - Oecetis sp. - - - - - - - - - - - - - - - - - - - - - - * - - - - - - - Nepidae Ranatra nigra - - - - - - - - - - - - - - - - - - - - - - * - - * - * - - Malacostraca Amphipoda Corophiidae Apocorophium lacustre - - - - - - - - - * - - - * * - - - - * - * - - * - - - - * Apocorophium louisianum - - - - * - - - * * * * * * * * * * * * * * * - * - * - - - Apocorophium sp. * - - - * * - * * * * * * * * * * * * * * * * * * - * - - * Gammaridae Gammarus tigrinus * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Haustoriidae Lepidactylus dytiscus - - - - - - - * - - - - - - - - - - - - - - - - - - - - - - Talitridae Uhlorchestia (Orchestia) uhleri - * - - - * - - - - - - - - - - - - - - - - * - * - - - - - Daphniidae Daphnia sp. V - - - - - V - - - - - - - - - - - - - - - - - - - - - 1-7 Table 1-2 (concluded). Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Decapoda Cambaridae sp. - V - - - - - - - - - - - - - - - - - - - - - - Palaemonidae Palaemonetes pugio - - - - * * - * * * * * * * * * * * * * * * * * - - * * - * Panopeidae Rhithropanopeus harrisii - - - - - - - * - * - - - * * * - - - - * * - * - - - - - * Sesarmidae Sesarma reticulatum - - - - - - - - - - - - - - - - - - - - - - - ✓ - - - - - - Isopoda Anthuridae Cyathura polita - - - * - * - * - * - - * * - - * * * - - - - - * * * * - * Bopyroidea Probopyrus pandalicola - - - - - V - - - - - - - V - - - - - - - - - - - - - - - - Idoteidae Edotia montosa - - - - - - - - - - - - * * - * * * - - - * - - - - - - - - Sphaeromatidae Cassidinidea ovalis - V V V V V V - V V V V V V V V V - V V - - V V V V V V - V Mysida Mysidae Americamysis almyra - - - - * * - - - * - - * * * * - * * * - - - - - - - - - * Ostracoda Candonidae Candonidae sp. - - - - - - - - - - - - - - ✓ - - - ✓ - - - - - - - ✓ - - ✓ Cytherideidae Cyprideis littoralis - V V V V V V V V V V V - V V V V V V V V - V V V V V V V V Tanaidacea Leptocheliidae Hargeria rapax - - - - - * - - - * - * - * - * * * - * - * - - - - - * - * Maxillopoda Sessila Balanidae Balanus subalbidus - - - - - * - * - - - - - - - * - * - * - - - * - - * - - * Balanus improvisus - - - - - - - - - * - - - - - - - - - - - - - - - - - - - - Mollusca Bivalvia sp. - - V - - V V V V V V V - - V V - - - - V V V - - - Dreissenidae Mytilopsis leucophaeata * * - * * - * * - - - - - - - - - - - - - - * - * - - - * - Mytilidae Geukensia demissa - - - - - - - - - - - - - - - - * - - * - - - - - - - - - - Gastropoda Basommatophora Planorbidae sp. - - - - - - ✓ - - - - - - - - - - - - - - - - - - - - - - - Physidae sp. (physalphysella) * * * - - - * - - - - - - - - - - - - - - - - - - - - - - - Hydrobiidae Littoridinops sp. * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Totaltaxa 26 29 28 28 31 19 29 16 27 32 27 30 28 25 24 26 24 22 24 23 17 15 33 16 29 24 24 23 19 24 — = Species not collected at station 1-8 Table 1-3. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Broomfield Creek upstream station in Ma v 2021. Species/taxona Annelida Clitellata Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Enchytraeidae sp. 2 1.0 1 1.0 Naididae sp. w/o hair 82 1.0 30 30.0 Limnodrilus hoffineisteri 2 1.0 1 1.0 Naididae sp. w/ hair 3 1.0 3 3.0 Dero sp. 5 1.0 3 3.0 Arthropoda Insecta Diptera Callibaetis sp. 6 1.1 3 3.3 Chironomus decorus 4 1.0 3 3.0 Cryptochironomus sp. 4 1.0 3 3.0 Dicrotendipes nervosus 2 1.0 1 1.0 Polypedilum tritum 5 1.3 3 3.9 Procladius sp. 2 1.2 1 1.2 Tanytarsus sp. 12 2.3 10 23.0 Hemiptera Abedus/Belostoma sp. 1 1.0 1 1.0 Corixidae sp. 108 1.3 100 130.0 Odonata Enallagma sp. 3 1.5 3 4.5 Malacostraca Amphipoda Apocophorium sp. 1 2.2 1 2.2 Gammarus tigrinus 4 2.0 3 6.0 Mollusca Bivalvia Mytilopsis leucophaeta 11 1.5 10 15.0 Gastropoda Littoridinops sp. 1 2.0 1 2.0 Physidae sp. (physa/physella) 6 1.3 3 3.9 Total 184 241.0 Estuarine Biotic Index = 1.31 Total taxa = 26 Total taxa with sensitivity values = 18 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. 1-9 Table 1-4. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Broomfield Creek downstream station in Ma v of 2021. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 4 1.0 3 3.0 Naididae sp. 6 1.0 3 3.0 Naididae sp. (w/o hair) 33 1.0 30 30.0 Limnodrilus hoffmeisteri 6 1.0 3 3.0 Nais sp. 5 1.3 3 3.9 Paranais littoralis 1 1.1 1 1.1 Polychaeta Amphicteis floridus 4 2.0 3 6.0 Laeoneris culveri 1 1.0 1 1.0 Arthropoda 1 nsecta Coleoptera Peltodytes sp. 1 1.4 1 1.4 Diptera Bezzia/Palpomyia complex 13 2.3 10 23.0 Chironomus decorus 10 1.0 10 10.0 Cricotopus sp. 1 1.4 1 1.4 Dicrotendipes nervosus 40 1.0 30 30.0 Polypedilum tritum 8 1.3 3 3.9 Tanytarsus sp. 70 2.3 30 69.0 Tanypus neopunctipennis 11 1.0 10 10.0 Hemiptera Abedus/Belostoma sp. 1 1.0 1 1.0 Corixidae sp. 30 1.3 30 39.0 Sigara sp. 1 1.4 1 1.4 Odonata Enallagma sp. 1 1.5 1 1.5 Perithemis tenera 1 1.0 1 1.0 Malacostraca Amphipoda Gammarus tigrinus 89 2.0 30 60.0 Uhlorchestia (Orchestia) uhleri 1 2.0 1 2.0 Mollusca Bivalvia Mytilopsis leucophaeta 32 1.5 30 45.0 Gastropoda Littoridinops sp. 7 2.0 3 6.0 Physidae sp. (physa/physella) 3 1.3 3 3.9 Total 243 360.5 Estuarine Biotic Index = 1.48 Total taxa = 29 Total taxa with sensitivity values = 22 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. 1-10 Table 1-5. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the SCUT1 upstream station in May 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 207 1.0 100 100.0 Limnodrilus hoffineisteri 33 1.0 30 30.0 Arthropoda Insecta Coleoptera Clinotanypus sp. 1 1.4 1 1.4 Peltodytes sp. 4 1.4 3 4.2 Diptera Bezzia/Palmonyia complex 4 2.3 3 6.9 Chironomus decorus 61 1.0 30 30.0 Cryptochironomus sp. 7 1.0 3 3.0 Dicrotendipes nervosus 6 1.0 3 3.0 Glyptotendipes sp. 4 1.3 3 3.9 Polypedilum tritum 9 1.3 3 3.9 Procladius sp. 2 1.2 1 1.2 Tanypus neopunctipennis 21 1.0 10 10.0 Tanytarsus sp. 43 2.3 30 69.0 Hemiptera Corixidae sp. 164 1.3 100 130.0 Odonata Enallagma sp. 3 1.5 3 4.5 Malacostraca Amphipoda Gammarus tigrinus 2 2.0 1 2.0 Mollusca Gastropoda Littoridinops sp. 2 2.0 1 2.0 Physidae sp. (physa/physella) 6 1.3 3 3.9 Total 228 308.9 Estuarine Biotic Index = 1.25 Total taxa = 28 Total taxa with sensitivity values = 17 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. Table 1-6. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the SCUT1 downstream station in May of 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. (w/hair) Dero sp. 1 1.0 1 1 Naididae sp. (w/o hair) Limnodrilus hoffmeisteri 1 1.0 1 1 Paranais litoralis 3 1.1 3 3.3 Polychaeta Amphicteis floridus 41 2.0 30 60.0 Laeonereis culveri 1 1.0 1 1.0 Arthropoda 1 nsecta Coleoptera Berosus sp. 2 1.5 1 1.5 Peltodytes sp. 2 1.4 1 1.4 Diptera Chironomus decorous 13 1.0 10 10.0 Dicrotendipes nervosus 28 1.0 10 10.0 Nanocladius sp. 1 2.0 1 2.0 Parachironomus sp. 1 1.1 1 1.1 Polypedilum tritum 3 1.3 3 3.9 Procladius sp. 5 1.2 3 3.6 Tanypus neopunctipennis 1 1.0 1 1.0 Tanytarsus sp. 13 2.3 10 23.0 Hemiptera Corixidae sp. 1 1.3 1 1.3 Malacostraca Amphipoda Gammarus tigrinus 300 2.0 100 200.0 Isopoda Cyathura polita 1 2.0 1 2.0 Mollusca Bivalvia Mytilopsis leucophaeta 5 1.5 3 4.5 Gastropoda Littoridinops sp. 23 2.0 10 20.0 Total 192 351.6 Estuarine Biotic Index = 1.83 Total taxa = 28 Total taxa with sensitivity values = 20 'Only those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-12 Table 1-7. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Jacks Creek upstream station in May 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 5 1.0 3 3.0 Naididae sp. w/o hair 5 1.0 3 3.0 Polychaeta Amphicteis floridus 31 2.0 30 60.0 Arthropoda Insecta Coleoptera Berosus sp. 14 1.5 10 15.0 Peltodytes sp. 2 1.4 1 1.4 Diptera Bezzia/Palmonyia complex 3 2.3 3 6.9 Chironomus decorus 47 1.0 30 30.0 Cladotanytarsus sp. 2 3.9 1 3.9 Cricotopus sp. 7 1.4 3 4.2 Dicrotendipes nervosus 18 1.0 10 10.0 Dolichopodidae sp. 1 1.0 1 1.0 Endochironomus sp. 5 1.1 3 3.3 Glyptotendipes sp. 1 1.3 1 1.3 Goeldichironomus devineyae 44 2.0 30 60.0 Parachironomus sp. 5 1.1 3 3.3 Tanypus neopunctipennis 14 1.0 10 10.0 Tanytarsus sp. 15 2.3 10 23.0 Hemiptera Corixidae sp. 4 1.3 3 3.9 Pelocoris femoratus 1 2.3 1 2.3 Odonata Enallagma sp. 4 1.5 3 4.5 Malacostraca Amphipoda Apocorophium louisianum 23 2.2 10 22.0 Apocorophium sp. 251 2.2 100 220.0 Gammarus tigrinus 123 2.0 100 200.0 Decapoda Palaemonetes pugio 13 2.5 10 25.0 Mysida Americamysis almyra 4 2.2 3 6.6 Mollusca Bivalvia Mytilopsis leucophaeata 2 1.5 1 1.5 Gastropoda Littoridinops sp. 257 2.0 100 200.0 Total 483 925.1 Estuarine Biotic Index = 1.92 Total taxa = 31 Total taxa with sensitivity values = 26 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. 1-13 Table 1-8. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Jacks Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)° Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 8 1.0 3 3.0 Polychaeta Amphecteis floridus 1 2.0 1 2.0 Arthropoda Diptera Chironomus decorus 2 1.0 1 1.0 Dicrotendipes nervosus 3 1.0 3 3.0 Goeldichironomus devineyae 14 2.0 10 20.0 Hemiptera sp. Corixidae sp. 1 1.3 1 1.3 Malacostraca Amphipoda Apocophorium sp. 3 2.2 3 6.6 Gammarus tigrinus 36 2.0 30 60.0 Orchestia uhleri 1 2.0 1 2.0 Decapoda Palaemonetes pugio 16 2.5 10 25.0 Isopoda Cyathura polita 1 2.0 1 2.0 Mysida Americamysis almyra 7 2.2 3 6.6 Tanaidacea Hargeria rapax 5 1.6 3 4.8 Maxillopoda Sessila Balanus subalbidus 2 2.0 1 2.0 Mollusca Gastropoda Littoridinops sp. 204 2.0 100 200.0 Total 171 339.3 Estuarine Biotic Index = 1.98 Total taxa = 19 Total taxa with sensitivity values = 15 aOnly those taxa for which a sensitivity value is available are displayed. "Total number of individuals caught in all three sweep replicates. °Sensitivity values provided by NCDWR. 1-14 Table 1-9. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Little Creek upstream station in May 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 4 1.0 3 3.0 Naididae sp. w/o hair 32 1.0 30 30.0 Limnodrilus hoffmeisteri 10 1.0 10 10.0 Nais sp. 2 1.3 1 1.3 Polychaeta Amphicteis floridus 43 2.0 30 60.0 Laeoenreis culveri 3 1.0 3 3.0 Arthropoda 1 nsecta Coleptera Peltodytes sp. 1 1.4 1 1.4 Diptera Bezzia/Palpomyia complex 1 2.3 1 2.3 Chironomus decorus 131 1.0 100 100.0 Dicrotendipes nervosus 20 1.0 10 10.0 Dolichopodidae sp. 1 1.0 1 1.0 Goeldichironomus devineyae 1 2.0 1 2.0 Polypedilum sp. 5 1.5 3 4.5 Procladius sp. 5 1.2 3 3.6 Tanypus neopunctipennis 4 1.0 3 3.0 Tanytarsus sp. 58 2.3 30 69.0 Ephemeroptera Callibaetis sp. 1 1.1 1 1.1 Hemiptera Corixidae sp. 9 1.3 3 3.9 Odonata Perithemis tenera 2 1.0 1 1.0 Malacostraca Amphipoda Gammarus tigrinus 46 2.0 30 60.0 Mollusca Bivalvia Mytilopsis leucophaeata 2 1.5 1 1.5 Gastropoda Littoridinops sp. 16 2.0 10 20.0 Physidae sp. (physa/physella) 7 1.3 3 3.9 Total 279 395.5 Estuarine Biotic Index = 1.42 Total taxa = 29 Total taxa with sensitivity values = 20 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. 1-15 Table 1-10. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Little Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 2 1.0 1.0 1.0 Naididae sp. (w/o hair) 5 1.0 3.0 3.0 Polychaeta Amphicteis floridus 3 2.0 3 6.0 Arthropoda 1 nsecta Diptera Dicrotendipes nervosus 1 1.0 1 1.0 Goeldichironomus devineyae 3 2.0 3 6.0 Malacostraca Apocorophium sp. 1 2.2 1 2.2 Gammarus tigrinus 6 2.0 3 6.0 Lepidactylus dytiscus 1 3.5 1 3.5 Decapoda Palaemonetes pugio 15 2.5 10 25.0 Rhithropanopeus harrisii 2 2.5 1 2.5 Isopoda Cyathura polita 1 2.0 1 2.0 Maxillopoda Sessilia Balanus subalbidus 5 2.0 3 6.0 Mollusca Bivalvia Mytilopsis leucophaeata 1 1.5 1 1.5 Gastropoda Littoridinops sp. 13 2.0 10 20.0 Total 42 85.7 Estuarine Biotic Index = 2.04 Total taxa = 16 Total taxa with sensitivity values = 14 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-16 Table 1-11. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Jacobs Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 22 1.0 10 10.0 Naididae sp. w/o hair 16 1.0 10 10.0 Limnodrilus hoffineisteri 2 1.0 1 1.0 Polychaeta Amphicteis floridus 15 2.0 10 20.0 Laeoenreis culveri 10 1.0 10 10.0 Arthropoda Insecta Coleoptera Berosus sp. 25 1.5 10 15.0 Peltodytes sp. 1 1.4 1 1.4 Diptera Apedilum sp. 1 4.0 1 4.0 Bezzia/Palpomyia Complex 5 2.3 3 6.9 Chironomus decorus 22 1.0 10 10.0 Chrysops sp. 3 2.2 3 6.6 Cricotopus sp. 14 1.4 10 14.0 Dicrotendipes nervosus 1 1.0 1 1.0 Dolichopodidae sp. 1 1.0 1 1.0 Goeldichironomus devineyae 165 2.0 100 200.0 Parachironomus sp. 3 1.1 3 3.3 Tanytarsus sp. 28 2.3 10 23.0 Hemiptera Abedus/Belostoma sp. 1 1.0 1 1.0 Odonata Enallagma sp. 2 1.5 1 1.5 Malacostraca Amphipoda Apocorophium louisianum 15 2.2 10 22.0 Apocorophium sp. 79 2.2 30 66.0 Gammarus tigrinus 2 2.0 1 2.0 Decapoda Palaemonetes pugio 59 2.5 30 75.0 Mollusca Gastropoda Littoridinops sp. 300 2.0 100 200.0 Total 367 704.7 Estuarine Biotic Index = 1.92 Total taxa = 27 Total taxa with sensitivity values = 22 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. 1-17 Table 1-12. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Jacobs Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 1 1.0 1 1.0 Naididae (Tubificinae) sp. w/o hair 10 1.0 10 10.0 Polychaeta Amphicteis floridus 54 2.0 30 60.0 Laeonereis culveri 4 1.0 3 3.0 Streblospio benedicti 4 1.1 3 3.3 Arthropoda Insecta Diptera Apedilum sp. 1 4.0 1 4.0 Bezzia/Palpomyia Complex 3 2.3 3 6.9 Chironomus decorus 80 1.0 30 30.0 Cricotopus sp. 3 1.4 3 4.2 Dicrotendipes nervosus 52 1.0 30 30.0 Endochironomus sp. 2 1.1 1 1.1 Glyptotendipes sp. 4 1.3 3 3.9 Goeldichironomus devineyae 94 2.0 30 60.0 Nanocladius sp. 2 2.0 1 2.0 Tanytarsus sp. 31 2.3 30 69.0 Hemiptera sp. Trichocorixa sexcinta 1 1.0 1 1.0 Odonata Enallagma sp. 1 1.5 1 1.5 Malacostraca Amphipoda Apocorophium lacustre 2 2.2 1 2.2 Apocorophium louisianum 5 2.2 3 6.6 Apocorophium sp. 40 2.2 30 66.0 Gammarus tigrinus 121 2.0 100 200.0 Decapoda Palaemonetes pugio 34 2.5 30 75.0 Rhithropanopeus harrisii 5 2.5 3 7.5 Isopoda Cyathura polita 2 2.0 1 2.0 Mysida Americamysis almyra 20 2.2 10 22.0 Maxillopoda Sessilia Balanus improvisus 4 2.0 3 6.0 Tanaidacea Hargeria rapax 135 1.6 100 160.0 Mollusca Gastropoda Littoridinops sp. 175 2.0 100 200.0 Total 562 1,038.2 Estuarine Biotic Index = 1.85 Total taxa = 32 Total taxa with sensitivity values = 26 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. 1-18 Table 1-13. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the PA2 Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 6 1.0 3 3.0 Polychaeta Amphicteis floridus 36 2.0 30 60.0 Arthropoda 1 nsecta Coleoptera Berosus sp. 5 1.5 3 4.5 Peltodytes sp. 3 1.4 3 4.2 Diptera Apedilum sp. 2 4.0 1 4.0 Bezzia/Palpomyia complex 2 2.3 1 2.3 Chironomus decorus 140 1.0 100 100.0 Cricotopus sp. 41 1.4 30 42.0 Dicrotendipes nervosus 11 1.0 10 10.0 Goeldichironomus devineyae 252 2.0 100 200.0 Nanocladius sp. 2 2.0 1 2.0 Parachironomus sp. 10 1.1 10 11.0 Tanypus neopunctipennis 10 1.0 10 10.0 Tanytarsus sp. 19 2.3 10 23.0 Hemiptera sp. Callibaetis sp. 13 1.1 10 11.0 Corixidae sp. 43 1.3 30 39.0 Trichocorixa sexcinta 5 1.0 3 3.0 Odonata Enallagma sp. 4 1.5 3 4.5 Libellula sp. 3 1.1 3 3.3 Malacostraca Amphipoda Apocorophium louisianum 10 2.2 10 22.0 Apocorophium sp. 140 2.2 100 220.0 Gammarus tigrinus 54 2.0 30 60.0 Decapoda Palaemonetes pugio 16 2.5 10 25.0 Mollusca Gastropoda Littoridinops sp. 294 2.0 100 200.0 Total 611 1,063.8 Estuarine Biotic Index = 1.74 Total taxa = 27 Total taxa with sensitivity values = 23 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-19 Table 1-14. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the PA2 Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 2 1.0 1 1.0 Polychaeta Amphicteis floridus 49 2.0 30 60.0 Laeonereis culveri 2 1.0 1 1.0 Arthropoda 1 nsecta Coleoptera Berosus sp. 8 1.5 3 4.5 Peltodytes sp. 2 1.4 1 1.4 Diptera Bezzia/Palpomyia complex 8 2.3 3 6.9 Chironomus decorus 67 1.0 30 30.0 Cricotopus sp. 36 1.4 30 42.0 Dicrotendipes nervosus 57 1.0 30 30.0 Endochironomus sp. 3 1.1 3 3.3 Goeldichironomus devineyae 266 2.0 100 200.0 Nanocladius sp. 5 2.0 3 6.0 Parachironomus sp. 16 1.1 10 11.0 Tanypus neopunctipennis 2 1.0 1 1.0 Tanytarsus sp. 23 2.3 10 23.0 He mi pte ra Abedus/Belostoma sp. 2 1.0 1 1.0 Callibaetis sp. 2 1.1 1 1.1 Corixidae sp. 55 1.3 30 39.0 Trichocorixa sexcinta 14 1.0 10 10.0 Odonata Enallagma sp. 12 1.5 10 15.0 Libellula sp. 2 1.1 1 1.1 Malacostraca Amphipoda Apocorophium louisianum 16 2.2 10 22.0 Apocorophium sp. 83 2.2 30 66.0 Gammarus tigrinus 86 2.0 30 60.0 Mysida Hargeria rapax 6 1.6 3 4.8 Decapoda Palaemonetes pugio 8 2.5 3 7.5 Mollusca Gastropoda Littoridinops sp. 300 2.0 100 200.0 Total 485 848.6 Estuarine Biotic Index = 1.75 Total taxa = 30 Total taxa with sensitivity values = 26 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-20 Table 1-15. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Drinkwater Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 2 1.0 1 1.0 Naididae sp. w/o hair 6 1.0 3 3.0 Tubificoides heterochaetus 2 1.1 1 1.1 Polychaeta Amphicteis floridus 44 2.0 30 60.0 Laeonereis culveri 2 1.0 1 1.0 Arthropoda Insecta Coleoptera Berosus sp. 14 1.5 10 15.0 Peltodytes sp. 6 1.4 3 4.2 Diptera Bezzia/Palmonyia complex 6 2.3 3 6.9 Chironomus decorus 142 1.0 100 100.0 Dicrotendipes nervosus 36 1.0 30 30.0 Goeldichironomus devineyae 82 2.0 30 60.0 Nanocladius sp. 4 2.0 3 6.0 Parachironomus sp. 2 1.1 1 1.1 Tanytarsus sp. 70 2.3 30 69.0 Hemiptera Abedus/Belostoma sp. 2 1.0 1 1.0 Corixidae sp. 2 1.3 1 1.3 Odonata Enallagma sp. 4 1.5 3 4.5 Malacostraca Amphipoda Apocorophium louisianum 50 2.2 30 66.0 Apocorophium sp. 258 2.2 100 220.0 Gammarus tigrinus 260 2.0 100 200.0 Decapoda Palaemonetes pugio 22 2.5 10 25.0 Isopoda Cyathura polita 4 2.0 3 6.0 Edotia montosa 2 2.6 1 2.6 Mysida Americamysis almyra 8 2.2 3 6.6 Mollusca Gastropoda Littoridinops sp. 220 2.0 100 200.0 Total 598 1,091.3 Estuarine Biotic Index = 1.82 Total taxa = 28 Total taxa with sensitivity values = 23 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. °Sensitivity values provided by NCDWR. 1-21 Table 1-16. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Drinkwater Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (TV)° Abundance value (n) Product of (TV)(n) Annelida Clitellata Enchytraeidae sp. 4 1.0 3 3.0 Naididae sp. w/o hair 4 1.0 3 3.0 Paranais sp. 1 1.1 1 1.1 Tubificoides heterochaetus 1 1.1 1 1.1 Polychaeta Amphicteis floridus 31 2.0 30 60.0 Laeonereis culveri 4 1.0 3 3.0 Arthropoda Insecta Coleoptera Berosus sp. 4 1.5 3 4.5 Diptera Chironomus decorus 101 1.0 100 100.0 Dicrotendipes nervosus 42 1.0 30 30.0 Goeldichironomus devineyae 155 2.0 100 200.0 Polypedilum sp. 1 1.5 1 1.5 Tanytarsus sp. 69 2.3 30 69.0 Malacostraca Amphipoda Apocophorium lacustre 1 2.2 1 2.2 Apocophorium louisianum 8 2.2 3 6.6 Apocorophium sp. 11 2.2 10 22.0 Gammarus tigrinus 127 2.0 100 200.0 Decapoda Palaemonetes pugio 13 2.5 10 25.0 Rhithropanopeus harrisii 1 2.5 1 2.5 Isopoda Cyathura polita 2 2.0 1 2.0 Edotia montosa 1 2.6 1 2.6 Mysida Americamysis almyra 42 2.2 30 66.0 Tanaidacea Hargeria rapax 180 1.6 100 160.0 Mollusca Gastropoda Littoridinops sp. 167 2.0 100 200.0 Total 662 1,165.1 Estuarine Biotic Index = 1.76 Total taxa = 25 Total taxa with sensitivity values = 19 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. °Sensitivity values provided by NCDWR. 1-22 Table 1-17. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Long Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 4 1.0 3 3.0 Naididae sp. (w/o hair) 4 1.0 3 3.0 Polychaeta Amphicteis floridus 108 2.0 100 200.0 Streblospio benedicti 2 1.1 1 1.1 Arthropoda 1 nsecta Diptera Chironomus decorus 300 1.0 100 100.0 Cricotopus sp. 10 1.4 10 14.0 Dicrotendipes nervosus 14 1.0 10 10.0 Goeldichironomus devineyae 60 2.0 30 60.0 Parachironomus sp. 4 1.1 3 3.3 Tanypus neopunctipennis 28 1.0 10 10.0 Tanytarsus sp. 62 2.3 30 69.0 Hemiptera Corixidae sp. 44 1.3 30 39.0 Trichocorixa sexcinta 4 1.0 3 3.0 Odonata Enallagma sp. 2 1.5 1 1.5 Malacostraca Amphipoda Apocorophium lacustre 2 2.2 1 2.2 Apocorophium louisianum 36 2.2 30 66.0 Apocorophium sp. 158 2.2 100 220.0 Gammarus tigrinus 26 2.0 10 20.0 Decapoda Palaemonetes pugio 34 2.5 30 75.0 Rhithropanopeus harrisii 2 2.5 1 2.5 Mysida Americamysis almyra 88 2.2 30 66.0 Mollusca Gastropoda Littoridinops sp. 300 2.0 100 200.0 Total 636 1,168.6 Estuarine Biotic Index = 1.84 Total taxa = 24 Total taxa with sensitivity values = 20 'Only those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-23 Table 1-18. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Long Creek downstream station in Ma v 2021. Species/taxona Number of individuals" Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 2 1.0 1 1 Paranais litoralis 1 1.1 1 1.1 Polychaeta Amphicteis floridus 5 2.0 3 6.0 Arthropoda I nsecta Coleoptera sp. Peltodytes sp. 2 1.4 1 1.4 Diptera Chironomus decorus 73 1.0 30 30.0 Cricotopus sp. 5 1.4 3 4.2 Dicrotendipes nervosus 19 1.0 10 10.0 Goeldichironomus devineyae 29 2.0 10 20.0 Nanocladius sp. 2 2.0 1 2.0 Parachironomus sp. 4 1.1 3 3.3 Tanytarsus sp. 30 2.3 30 69.0 Ephemeroptera Callibaetis sp. 2 1.1 1 1.1 Hemiptera Corixidae sp. 2 1.3 1 1.3 Odonata Oecetis inconspicua 2 4.6 1 4.6 Malacostraca Amphipoda Apocorophium louisianum 4 2.2 3 6.6 Apocorophium sp. 35 2.2 30 66.0 Gammarus tigrinus 105 2.0 100 200.0 Decapoda Palaemonetes pugio 12 2.5 10 25.0 Rhithropanopeus harrisii 1 2.5 1 2.5 Isopoda Edotia montosa 1 2.6 1 2.6 Mysida Americamysis almyra 92 2.2 30 66.0 Tanaidacea Hargeria rapax 116 1.6 100 160.0 Maxillopoda Sessilia Balanus subalbidus 30 2.0 30 60.0 Mollusca Gastropoda Littoridinops sp. 98 2.0 30 60.0 Total 431 803.7 Estuarine Biotic Index = 1.86 Total taxa = 26 Total taxa with sensitivity values = 23 aOnly those taxa for which a sensitivity value is available are displayed. "Total number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. 1-24 Table 1-19. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Tooley Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 11 1.0 10 10.0 Naididae sp. (w/o hair) 4 1.0 3 3.0 Polychaeta Amphicteis floridus 172 2.0 100 200.0 Laeonereis culveri 20 1.0 10 10.0 Arthropoda 1 nsecta Diptera Bezzia/Palmonyia complex 4 2.3 3 6.9 Chironomus decorus 59 1.0 30 30.0 Cricotopus sp. 4 1.4 3 4.2 Dicrotendipes nervosus 81 1.0 30 30.0 Goeldichironomus devineyae 64 2.0 30 60.0 Nanocladius sp. 4 2.0 3 6.0 Tanypus neopunctipennis 4 1.0 3 3.0 Tanytarsus sp. 300 2.3 100 230.0 Malacostraca Amphipoda Apocorophium louisianum 124 2.2 100 220.0 Apocorophium sp. 260 2.2 100 220.0 Gammarus tigrinus 204 2.0 100 200.0 Decapoda Palaemonetes pugio 18 2.5 10 25.0 Isopoda Cyathura polita 35 2.0 30 60.0 Edotia montosa 3 2.6 3 7.8 Tanaidacea Hargeria rapax 288 1.6 100 160.0 Mollusca Bivalvia Geukensia demissa 3 3.4 3 10.2 Gastropoda Littoridinops sp. 300 2.0 100 200.0 Total 871 1,696.1 Estuarine Biotic Index = 1.95 Total taxa = 24 Total taxa with sensitivity values = 20 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-25 Table 1-20. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Tooley Creek downstream station in Ma v of 2021. Species/taxona Number of individualsb Sensitivity value (SV)° Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. (w/o hair) 2 1.0 1 1.0 Polychaeta Amphicteis floridus 41 2.0 30 60.0 Laeonereis culveri 1 1.0 1 1.0 Arthropoda Insecta Coleoptera Peltodytes sp. 4 1.4 3 4.2 Diptera Chironomus decorus 44 1.0 30 30.0 Dicrotendipes nervosus 44 1.0 30 30.0 Goeldichironomus devineyae 23 2.0 10 20.0 Nanocladius sp. 5 2.0 3 6.0 Parachironomus sp. 10 1.1 10 11.0 Tanytarsus sp. 102 2.3 100 230.0 Hemiptera Corixidae sp. 4 1.3 3 3.9 Odonata Enallagma sp. 4 1.5 3 4.5 Malacostraca Amphipoda Apocorophium louisianum 3 2.2 3 6.6 Apocorophium sp. 87 2.2 30 66.0 Gammarus tigrinus 101 2.0 100 200.0 Decapoda Palaemonetes pugio 52 2.5 30 75.0 Isopoda Cyathura polita 3 2.0 3 6.0 Edotia montosa 13 2.6 10 26.0 Mysida Americamysis almyra 34 2.2 30 66.0 Tanaidacea Hargeria rapax 120 1.6 100 160.0 Maxillopoda Sessilia Balanus subalbidus 2 2.0 1 2.0 Mollusca Gastropoda Littoridinops sp. 200 2.0 100 200.0 Total 631 1,209.2 Estuarine Biotic Index = 1.92 Total taxa = 22 Total taxa with sensitivity values = 21 aOnly those taxa for which a sensitivity value is available are displayed. "Total number of individuals caught in all three sweep replicates. °Sensitivity values provided by NCDWR. 1-26 Table 1-21. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Muddy Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 8 1.0 3 3.0 Naididae sp. (w/o hair) 6 1.0 3 3.0 Polychaeta Amphicteis floridus 135 2.0 100 200.0 Laeonereis culveri 1 1.0 1 1.0 Arthropoda 1 nsecta Coleoptera Berosus sp. 9 1.5 3 4.5 Diptera Chironomus decorus 56 1.0 30 30.0 Cricotopus sp. 1 1.4 1 1.4 Dicrotendipes nervosus 26 1.0 10 10.0 Goeldichironomus devineyae 71 2.0 30 60.0 Nanocladius sp. 2 2.0 1 2.0 Tanytarsus sp. 94 2.3 30 69.0 Hemiptera Corixidae sp. 4 1.3 3 3.9 Malacostraca Amphipoda Apocorophium louisianum 71 2.2 30 66.0 Apocorophium sp. 243 2.2 100 220.0 Gammarus tigrinus 251 2.0 100 200.0 Decapoda Palaemonetes pugio 58 2.5 30 75.0 Isopoda Cyathura polita 2 2.0 1 2.0 Mysida Americamysis almyra 1 2.2 1 2.2 Mollusca Gastropoda Littoridinops sp. 275 2.0 100 200.0 Total 577 1,153.0 Estuarine Biotic Index = 2.00 Total taxa = 24 Total taxa with sensitivity values = 18 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-27 Table 1-22. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Muddy Creek downstream station in May 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 1 1.0 1 1.0 Polychaeta Amphicteis floridus 56 2.0 30 60.0 Polydora cornuta 2 1.2 1 1.2 Arthropoda Insecta Diptera Chironomus decorus 65 1.0 30 30.0 Dicrotendipes nervosus 5 1.0 3 3.0 Goeldichironomus devineyae 24 2.0 10 20.0 Parachironomus sp. 1 1.1 1 1.1 Tanytarsus sp. 14 2.3 10 23.0 Hemiptera Corixidae sp. 4 1.3 3 3.9 Trichocorixa sexcinta 1 1.0 1 1.0 Odonata Enallagma sp. 1 1.5 1 1.5 Malacostraca Amphipoda Apocorophium lacustre 4 2.2 3 6.6 Apocorophium louisianum 11 2.2 10 22.0 Apocorophium sp. 160 2.2 100 220.0 Gammarus tigrinus 191 2.0 100 200.0 Decapoda Palaemonetes pugio 88 2.5 30 75.0 Mysida Americamysis almyra 9 2.2 3 6.6 Tanaidacea Hargeria rapax 2 1.6 1 1.6 Maxillopoda Sessilia Balanus subalbidus 1 2.0 1 2.0 Mollusca Bivalvia Geukensia demissa 1 3.4 1 3.4 Gastropoda Littoridinops sp. 259 2.0 100 200.0 Total 440 882.9 Estuarine Biotic Index = 2.01 Total taxa = 23 Total taxa with sensitivity values =19 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. 1-28 Table 1-23. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Huddles Cut upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Tubificoides heterochaetus 8 1.1 3 3.3 Polychaeta Amphicteis floridus 27 2.0 10 20.0 Neanthes succinea 1 1.7 1 1.7 Arthropoda 1 nsecta Diptera Bezzia/Palpomyia complex 2 2.3 1 2.3 Chironomus decorus 75 1.0 30 30.0 Goeldichironomus devineyae 8 2.0 3 6.0 Procladius sp. 1 1.2 1 1.2 Tanypus neopunctipennis 1 1.0 1 1.0 Tanytarsus sp. 28 2.3 10 23.0 Malacostraca Amphipoda Apocorophium louisianum 6 2.2 3 6.6 Apocorophium sp. 24 2.2 10 22.0 Gammarus tigrinus 3 2.0 3 6.0 Decapoda Palaemonetes pugio 8 2.5 3 7.5 Rhithropanopeus harrisii 4 2.5 3 7.5 Mollusca Gastropoda Littoridinops sp. 23 2.0 10 20.0 Total 92 158.1 Estuarine Biotic Index = 1.72 Total taxa = 17 Total taxa with sensitivity values = 14 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-29 Table 1-24. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Huddles Cut downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 1 1.0 1 1.0 Polychaeta Amphicteis floridus 2 2.0 1 2.0 Arthropoda 1 nsecta Diptera Chironomus sp. 1 1.0 1 1.0 Cricotopus sp. 7 1.4 3 4.2 Dicrotendipes nervosus 7 1.0 3 3.0 Goeldichironomus devineyae 2 2.0 1 2.0 Parachironomus sp. 1 1.1 1 1.1 Tanytarsus sp. 2 2.3 1 2.3 Malacostraca Amphipoda Apocorophium lacustre 4 2.2 3 6.6 Apocorophium louisianum 1 2.2 1 2.2 Apocorophium sp. 8 2.2 3 6.6 Gammarus tigrinus 90 2.0 30 60.0 Decapoda Palaemonetes pugio 7 2.5 3 7.5 Rhithropanopeus harrisii 2 2.5 1 2.5 Isopoda Edotia montosa 1 2.6 1 2.6 Hargeria rapax 2 1.6 1 1.6 Mollusca Gastropoda Littoridinops sp. 3 2.0 3 6.0 Total 58 112.2 Estuarine Biotic Index = 1.93 Total taxa = 15 Total taxa with sensitivity values = 15 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-30 Table 1-25. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Porter Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 7 1.0 3 3.0 Naididae sp. w/o hair 31 1.0 30 30.0 Limnodrilus hoffmeisteri 6 1.0 3 3.0 Polychaeta Amphicteis floridus 115 2.0 100 200.0 Laeonereis culveri 2 1.0 1 1.0 Arthropoda Insecta Coleoptera Berosus sp. 1 1.5 1 1.5 Diptera Bezzia/Palpomyia complex 3 2.3 3 6.9 Chironomus decorus 11 1.0 10 10.0 Cricotopus sp. 4 1.4 3 4.2 Dicrotendipes nervosus 8 1.0 3 3.0 Dolichopodidae sp. 1 1.0 1 1.0 Endochironomus sp. 1 1.1 1 1.1 Goeldichironomus devineyae 40 2.0 30 60.0 Nanocladius sp. 6 2.0 3 6.0 Parachironomus sp. 1 1.1 1 1.1 Polypedilum sp. 1 1.5 1 1.5 Tanytarsus sp. 6 2.3 3 6.9 Ephemeroptera Callibaetis sp. 3 1.1 3 3.3 Hemiptera Abedus/Belostoma complex 4 1.0 3 3.0 Corixidae sp. 11 1.3 10 13.0 Ranatra nigra 2 2.6 1 2.6 Trichocorixa sexcinta 2 1.0 1 1.0 Odonata Enallagma sp. 5 1.5 3 4.5 Oecetis sp. 1 2.5 1 2.5 Malacostraca Amphipoda Apocorophium louisianum 9 2.2 3 6.6 Apocorophium sp. 25 2.2 10 22.0 Gammarus tigrinus 263 2.0 100 200.0 Orchestia uhleri 1 2.0 1 2.0 Decapoda Palaemonetes pugio 17 2.5 10 25.0 Mollusca Veneroida Mytilopsis leucophaeata 1 1.5 1 1.5 Gastropoda Littoridinops sp. 244 2.0 100 200.0 Total 444 827.2 Estuarine Biotic Index = 1.86 Total taxa = 33 Total taxa with sensitivity values = 29 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. °Sensitivity values provided by NCDWR. 1-31 Table 1-26. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Porter Creek downstream station in Ma v of 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 1 2.0 1 2.0 Arthropoda 1 nsecta Diptera Dicrotendipes nervosus 1 1.0 1 1.0 Goeldichironomus devineyae 10 2.0 10 20.0 Parachironomus sp. 2 1.1 1 1.1 Hemiptera Pelocoris femoratus 1 2.3 1 2.3 Malacostraca Amphipoda Apocorophium sp. 4 2.2 3 6.6 Gammarus tigrinus 22 2.0 10 20.0 Decapoda Palaemonetes pugio 24 2.5 10 25.0 Rhithropanopeus harrisii 1 2.5 1 2.5 Maxillopoda Sessilia Balanus subalbidus 25 2.0 10 20.0 Mollusca Gastropoda Littoridinops sp. 89 2.0 30 60.0 Total 78 160.5 Estuarine Biotic Index = 2.06 Total taxa = 16 Total taxa with sensitivity values = 11 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-32 Table 1-27. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the DCUT11 upstream station in May 2021. Number of Sensitivity Abundance Product of Species/taxona individualsb value (SV)c value (n) (SV)(n) Annelida Clitellata Enchytraeidae sp. 4 1.0 3 3.0 Naididae sp. w/o hair 18 1.0 10 10.0 Polychaeta Amphicteis floridus 47 2.0 30 60.0 Laeonereis culveri 2 1.0 1 1.0 Arthropoda Insecta Coleoptera Berosus sp. 36 1.5 30 45.0 Peltodytes sp. 2 1.4 1 1.4 Diptera Bezzia/Palmonyia complex 4 2.3 3 6.9 Cricotopus sp. 5 1.4 3 4.2 Dicrotendipes nervosus 102 1.0 100 100.0 Nanocladius sp. 1 2.0 1 2.0 Tabanus sp. 2 1.4 1 1.4 Tanytarsus sp. 66 2.3 30 69.0 Hemiptera Abedus/Belostoma sp. 1 1.0 1 1.0 Corixidae sp. 2 1.3 1 1.3 Odonata Enallagma sp. 2 1.5 1 1.5 Libellula sp. 1 1.1 1 1.1 Malacostraca Amphipoda Apocophorium lacustre 2 2.2 1 2.2 Apocophorium louisianum 2 2.2 1 2.2 Apocophorium sp. 1 2.2 1 2.2 Gammarus tigrinus 21 2.0 10 20.0 Orchestia uhleri 2 2.0 1 2.0 Isopoda Cyathura polita 2 2.0 1 2.0 Mollusca Bivalvia Mytilopsis leucophaeta 1 1.5 1 1.5 Gastropoda Littoridinops sp. 95 2.0 30 60.0 Total 263 400.9 Estuarine Biotic Index = 1.52 Total taxa = 29 Total taxa with sensitivity values = 22 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. 1-33 Table 1-28. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the DCUT11 downstream station in Ma v of 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 1 1.0 1 1.0 Naididae sp. (w/o hair) 3 1.0 3 3.0 Polychaeta Amphicteis floridus 6 2.0 3 6.0 Arthropoda Arachnida sp. 8 2.8 3 8.4 1 nsecta Coleoptera Berosus sp. 9 1.5 3 4.5 Diptera Bezzia/Palpomyia complex 3 2.3 3 6.9 Cricotopus sp. 60 1.4 30 42.0 Dicrotendipes nervosus 141 1.0 100 100.0 Nanocladius sp. 1 2.0 1 2.0 Polypedilum sp. 6 1.5 3 4.5 Procladius sp. 1 1.2 1 1.2 Tanytarsus sp. 39 2.3 30 69.0 Hemiptera Abedus/Belostoma sp. 8 1.0 3 3.0 Corixidae sp. 1 1.3 1 1.3 Ranatra nigra 8 2.6 3 7.8 Odonata Enallagma sp. 3 1.5 3 4.5 Malacostraca Amphipoda Gammarus tigrinus 15 2.0 10 20.0 Isopoda Cyathura polita 2 2.0 1 2.0 Mollusca Gastropoda Littoridinops sp. 59 2.0 30 60.0 Total 232 347.1 Estuarine Biotic Index = 1.50 Total taxa = 24 Total taxa with sensitivity values = 19 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-34 Table 1-29. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the DCUT19 upstream station in May 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 9 1.0 3 3.0 Naididae sp. w/o hair 7 1.0 3 3.0 Polychaeta Amphicteis floridus 31 2.0 30 60.0 Arthropoda 1 nsecta Coleoptera Berosus sp. 33 1.5 30 45.0 Di pte ra Bezzia/Palpomyia complex 4 2.3 3 6.9 Chironomus decorus 10 1.0 10 10.0 Dicrotendipes nervosus 52 1.0 30 30.0 Goeldichironomus devineyae 81 2.0 30 60.0 Parachironomus sp. 4 1.1 3 3.3 Polypedilum sp. 3 1.5 3 4.5 Tanytarsus sp. 78 2.3 30 69.0 Hemiptera Abedus/Belostoma sp. 2 1.0 1 1.0 Odonata Enallagma sp. 2 1.5 1 1.5 Malacostraca Amphipoda Apocorophium louisianum 4 2.2 3 6.6 Apocorophium sp. 2 2.2 1 2.2 Gammarus tigrinus 32 2.0 30 60.0 Decapoda Palaemonetes pugio 17 2.5 10 25.0 Isopoda Cyathura polita 2 2.0 1 2.0 Maxillopoda Sessilia Balanus subalbidus 1 2.0 1 2.0 Mollusca Gastropoda Littoridinops sp. 300 2.0 100 200.0 Total 323 595.0 Estuarine Biotic Index = 1.84 Total taxa = 24 Total taxa with sensitivity values = 19 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-35 Table 1-30. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the DCUT19 downstream station in May of 2021. Number of Sensitivity Abundance Product of Species/taxona individualsb value (SV)` value (n) (SV)(n) Annelida Clitellata Naididae sp. w/o hair 7 1.0 3 3.0 Polychaeta Amphicteis floridus 64 2.0 30 60.0 Laeonereis culveri 2 1.0 1 1.0 Arthropoda Arachnida sp. 2 2.8 1 2.8 Insecta Coleoptera Berosus sp. 3 1.5 3 4.5 Diptera Bezzia/Palpomyia complex 2 2.3 1 2.3 Chironomus decorus 51 1.0 30 30.0 Dicrotendipes nervosus 20 1.0 10 10.0 Goeldichironomus devineyae 27 2.0 10 20.0 Polypedilum sp. 1 1.5 1 1.5 Tanypus neopunctipennis 2 1.0 1 1.0 Tanytarsus sp. 29 2.3 10 23.0 Malacostraca Amphipoda Gammarus tigrinus 2 2.0 1 2.0 Decapoda Palaemonetes pugio 81 2.5 30 75.0 Hemiptera sp. Abedus/Belostoma sp. 1 1.0 1 1.0 Ranatra sp. 2 2.6 1 2.6 Isopoda Cyathura polita 2 2.0 1 2.0 Tanaidacea Hargeria rapax 2 1.6 1 1.6 Mollusca Gastropoda Littoridinops sp. 156 2.0 100 200.0 Total 236 443.3 Estuarine Biotic Index = 1.88 Total taxa = 23 Total taxa with sensitivity values = 19 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. °Sensitivity values provided by NCDWR. 1-36 Table 1-31. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Duck Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. 12 1.0 10 10.0 Naididae sp. w/hair 5 1.0 3 3.0 Dero sp. 38 1.0 30 30.0 Naididae sp. w/o hair 9 1.0 3 3.0 Nais sp. 6 1.3 3 3.9 Polychaeta Amphicteis floridus 20 2.0 10 20.0 Arthropoda I nsecta Coleoptera Berosus sp. 18 1.5 10 15.0 Diptera Bezzia/Palpomyia complex 5 2.3 3 6.9 Chironomus decorous 77 1.0 30 30.0 Dicrotendipes nervosus 282 1.0 100 100.0 Goeldichironomus devineyae 6 2.0 3 6.0 Nanocladius sp. 2 2.0 1 2.0 Tanypus neopunctipennis 14 1.0 10 10.0 Tanytarsus sp. 173 2.3 100 230.0 Hemiptera Corixidae sp. 23 1.3 10 13.0 Odonata Libellula sp. 3 1.1 3 3.3 Pachydiplax longipennis 3 1.0 3 3.0 Malacostraca Amphipoda Gammarus tigrinus 300 2.0 100 200.0 Mollusca Bivalvia Mytilopsis leucophaeata 134 1.5 100 150.0 Gastropoda Littoridinops sp. 227 2.0 100 200.0 Total 632 1,039.1 Estuarine Biotic Index = 1.64 Total taxa = 19 Total taxa with sensitivity values = 16 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. 'Sensitivity values provided by NCDWR. 1-37 Table 1-32. Estuarine Biotic Index (EBI) calculations for benthic sweep samples at the Duck Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 14 1.0 10.0 10.0 Naididae sp. w/ hair 1 1.0 1.0 1.0 Naididae sp. w/o hair 4 1.0 3 3.0 Polychaeta Amphicteis floridus 4 2.0 3 6.0 Arthropoda Insecta Diptera Chironomus decorus 8 1.0 3 3.0 Dicrotendipes nervosus 71 1.0 30 30.0 Nanocladius sp. 5 2.0 3 6.0 Polypedilum sp. 8 1.5 3 4.5 Tanytarsus sp. 44 2.3 30 69.0 Malacostraca Amphipoda Apocorophium lacustre 6 2.2 3 6.6 Apocorophium sp. 4 2.2 3 6.6 Gammarus tigrinus 151 2.0 100 200.0 Goeldichironomus devineyae 40 2.0 30 60.0 Decapoda Palaemonetes pugio 156 2.5 100 250.0 Rhithropanopeus harrisii 4 2.5 3 7.5 Isopoda Cyathura polita 4 2.0 3 6.0 Mysida Americamysis almyra 1 2.2 1 2.2 Maxillopoda Sessilia Balanus subalbidus 5 2.0 3 6.0 Tanaidacea Hargeria rapax 4 1.6 3 4.8 Mollusca Gastropoda Littoridinops sp. 40 2.0 30 60.0 Total 365 742.2 Estuarine Biotic Index = 2.03 Total taxa = 24 Total taxa with sensitivity values = 19 aOnly those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all three sweep replicates. `Sensitivity values provided by NCDWR. 1-38 Table 1-33. Raw data from benthic ponar grab samples collected in May 2021 at each station in all 15 creeks in the PCS study. Benthic organisms were not counted beyond 100 individuals in any sample. Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Annelida Hirudineasp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 4 -- -- -- -- -- -- Clitellata -- Enchytraeidae sp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 2 -- -- -- -- -- -- -- -- Naididae Naididae sp. w/ hair -- -- -- -- -- -- -- -- -- -- -- 7 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Naididae sp. w/o hair 140 13 113 2 -- -- -- 1 -- -- -- 1 -- -- -- -- 2 -- -- -- -- 73 -- -- -- -- -- 72 4 2 Limnodrilus hoffineisteri 24 8 26 -- -- -- 28 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Tubificoides heterochaetus -- -- -- 4 -- -- -- -- -- 3 -- -- -- 3 -- -- -- 1 -- -- -- -- -- -- -- -- -- -- -- -- Polychaeta Ampharetidae Amphicteis floridus -- 2 -- 102 9 16 202 -- 1 2 13 180 21 2 12 1 6 -- 11 3 -- -- 98 1 6 218 21 428 26 11 Capitellidae Heteromastus filiformis -- -- -- -- -- -- -- -- -- 4 -- -- -- -- -- -- -- 3 -- -- -- -- -- -- -- -- -- -- -- -- Mediomastus ambiseta -- -- -- -- -- -- -- -- -- 1 -- -- -- 6 -- -- -- 7 -- 1 -- -- -- 2 -- -- -- -- -- -- Nereididae Laeonereis culveri -- -- -- -- 29 12 -- 2 19 -- 28 187 8 2 -- -- 2 -- -- -- -- 3 -- -- -- 2 2 28 -- -- Neanthes succinea -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 1 -- -- -- -- -- -- -- -- -- -- Spionidae Marenzelleria viridis -- -- -- -- -- -- -- -- -- 1 -- -- -- -- -- -- 2 -- -- -- -- 26 -- -- -- -- -- -- -- -- Polydora cornuta -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 1 -- -- -- -- -- -- -- -- -- -- Streblospio benedicti -- -- -- -- 4 19 -- 13 -- 17 2 28 -- 30 7 47 14 40 -- 14 1 -- -- 8 -- -- -- -- -- 4 Arthropoda Insecta Coleoptera Hydrophilidae Berosus sp. -- -- -- -- 1 -- -- -- -- -- -- -- 1 -- -- -- -- -- -- -- -- -- -- -- -- 2 -- 8 -- -- Haliplidae Ablabesmyiasp. 2 -- 4 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Peltodytessp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 2 -- -- -- -- Diptera Ceratopogonidae Bezzia/Palpomyia complex -- -- 2 -- 6 -- 4 1 4 -- 6 5 5 -- -- 1 1 -- -- -- 4 -- 2 -- 3 6 11 4 8 2 Chaoboridae Chaoborus puctipennis 2 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Chironomidae Chironomus decorus 29 2 95 -- 77 467 252 500 104 430 244 138 75 445 457 442 228 432 219 431 164 -- 45 321 -- -- 234 8 12 132 1-39 Table 1-33. (continuedl. Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Chironomus stigmaterus -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 8 -- -- -- Chironomus p -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 2 -- -- -- -- -- -- -- -- -- -- -- -- -- Coelotanypus sp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 1 Cricotopus sp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 8 16 -- -- -- -- Cryptochironomus sp. 25 1 9 4 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Dicrotendipes nervosus 2 3 10 -- -- -- 6 -- -- -- 1 17 24 -- -- -- -- -- -- -- -- -- 2 -- 26 100 57 228 24 -- Endochironomus sp. -- -- -- -- -- -- 4 -- -- -- -- -- 1 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Goeldichironomus devineyae -- -- -- -- -- -- 2 -- -- 1 1 1 1 -- -- 1 -- -- -- -- -- -- -- -- -- -- -- 84 -- -- Kiefferulus sp. -- -- 2 -- 1 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Nanocladius sp. -- -- -- -- -- -- -- -- 1 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 9 6 7 4 -- -- Orthocladiinae sp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 2 -- -- -- -- Parachironomus sp. -- -- 3 -- -- -- 2 -- 5 -- -- 4 23 1 -- -- -- -- -- -- -- -- -- -- -- -- 2 17 -- -- -- Parakiefferiella coronata -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 6 -- -- -- -- Polypedilum sp. -- -- -- -- -- -- -- -- -- -- -- 2 -- -- -- -- -- -- -- -- 2 -- 1 -- 4 2 -- -- -- -- Polypedilum halterale 4 -- 28 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Polypedilum tritum -- -- 3 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Procladius sp. 29 2 27 2 -- -- 12 3 -- 1 -- -- -- 10 -- 1 -- 2 1 -- -- -- -- 5 -- 4 3 8 4 30 Tanypus neopunctipennis 29 2 57 1 50 10 -- 14 22 8 36 7 5 3 15 14 11 1 5 31 3 -- 3 -- -- 2 49 -- 2 3 Tanytarsus sp. -- 1 4 -- 1 -- -- -- 2 -- 1 59 9 -- -- -- 6 -- 3 1 1 5 5 -- 10 66 85 56 10 82 Tribelos jucundus 7 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Ephydridaesp. -- -- 2 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Tabanidae Chrysopssp. -- -- -- -- -- -- -- -- 2 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Hemiptera Corixidae Corixidae sp. 2 -- -- -- -- -- 2 -- -- -- 12 -- 4 -- -- -- -- -- -- -- -- -- -- -- -- 2 -- -- -- -- Trichocorixa kanza -- 1 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Odonata Coenagrionidae Enallagmasp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 1 Libellulidae Perithemis tenera -- -- 3 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Brachymesia furcata -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 2 -- -- -- -- Malacostraca Amphipoda Corophiidae Apocorophium lacustre -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 2 -- 8 -- -- Apocorophium louisianum -- -- -- -- -- -- -- -- -- -- -- 1 2 -- -- -- -- -- 4 -- -- -- 4 -- -- -- -- 8 -- -- Apocorophium sp. -- -- -- -- 1 -- -- -- -- -- -- 4 4 -- -- -- -- -- 3 -- -- -- 16 -- -- -- -- 12 -- -- Gammaridae Gammarus tigrinus -- 1 1 156 1 10 10 1 6 1 7 27 164 -- 10 1 -- 2 3 4 -- 4 42 1 13 124 2 500 12 7 Decapoda Palaemonidae Palaemonetes pugio -- -- -- -- -- -- -- -- -- -- -- -- 1 -- -- -- -- -- -- -- 1 -- -- -- -- -- 2 -- -- -- Panopeidae Rhithropanopeus harrisii -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 4 -- -- Isopoda Anthuridae Cyathura polita -- -- -- -- -- 1 -- -- -- -- -- 2 1 -- -- -- -- -- -- -- -- -- -- -- -- -- -- 76 -- -- Sphaeromatidae Cassidinidea ovalis -- -- -- -- -- -- -- -- -- -- -- -- 1 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 2 -- 1-40 Table 1-33. (concludedl. Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Idoteidae Edo ia mon osa -- -- -- -- -- -- -- -- -- 1 -- 1 -- -- -- 4 -- 1 1 -- -- -- 4 -- -- Mysida Mysidae Americamysis almyra 2 1 -- 2 3 2 -- 1 3 8 -- 8 4 1 4 -- 22 3 1 3 -- -- -- -- -- -- -- -- -- 1 Hargeria rapax -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 2 -- -- -- -- -- -- -- -- -- -- 404 -- -- Ostracoda Candonidae Candonidae sp. -- -- -- -- 1 -- -- -- -- -- -- 1 2 -- -- -- -- -- 5 1 -- -- 12 -- -- 2 114 52 8 -- Cytherideidae Cyprideis littoralis -- -- -- 4 2 6 2 -- -- -- 1 1 -- 1 3 -- 3 -- 1 -- -- -- -- -- -- 2 -- -- -- 4 Mollusca Bivalvia Bivalviasp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 1 -- -- -- -- -- -- Dreissenidae Mytilopsis leucophaeata -- -- -- -- -- -- 2 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- 2 -- -- -- 2 -- Mactridae Mulinia lateralis -- -- -- -- -- -- -- 2 -- 1 -- -- -- 1 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Tellinidae Macoma tenta -- -- -- -- -- -- -- -- -- 1 -- -- -- -- -- 1 -- 15 -- 2 1 -- -- 13 -- -- -- -- -- -- Gastropoda Basommatophora Physidae sp. (physa /physella) -- -- -- -- -- -- 2 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Hydrobiidae Littoridinops sp. -- -- -- -- 2 21 4 1 5 -- 18 62 9 4 2 2 11 5 -- 4 -- 4 2 4 23 54 118 44 8 5 Totaltaxa 12 11 16 8 15 10 15 11 12 15 14 21 20 12 8 10 14 11 10 14 9 8 11 9 10 22 15 19 13 14 — = Species not collected at station 1-41 Table 1-34. Taxa collected from benthic ponar grab samples collected in May 2021 in all 15 creeks in the PCS study. Taxa for which EBI was calculated are shown with a * and other are shown with a V. Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Annelida Hirudinea sp. -- -- -- -- -- -- -- ---- -- -- -- -- -- -- -- -- -- -- -- -- ✓ -- -- -- -- -- -- -- -- Clitellata Enchytraeidae sp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- * -- -- -- -- -- -- -- -- Naididae Naididaesp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Naididae sp. w/ hair -- -- -- -- -- -- -- -- -- -- -- * -- -- -- -- -- -- -- -- -- -- -- -- Naididae sp. w/o hair -- -- -- -- -- -- -- -- -- -- * -- -- -- -- * -- -- -- -- -- * * * Limnodrilus hoffineisteri * * * -- -- -- * -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Tubificoides heterochaetus -- -- -- * -- -- -- -- -- * -- -- -- * -- -- -- * -- -- -- -- -- -- Polychaeta Ampharetidae Amphicteis floridus -- * -- * * * * -- * * * * * * * * * -- * * -- -- * * * * * * * * Capitellidae Heteromastus filiformis -- -- -- -- -- -- -- -- -- * -- -- -- -- -- -- * -- -- -- -- -- -- -- Mediomastus ambiseta -- -- -- -- -- -- -- -- -- * -- -- -- * -- -- * -- * -- * -- -- -- -- Nereididae Laeonereis cu very -- -- -- -- -- * * * -- -- Neanthes succinea -- -- -- -- -- -- -- -- -- -- -- -- -- -- * -- -- -- -- -- -- -- -- -- Spionidae Marenzelleria viridis -- -- -- -- -- -- -- -- -- * -- -- -- -- -- -- * -- * -- -- -- -- -- -- Polydora cornuta -- -- -- -- -- -- -- -- -- ---- -- -- -- -- -- -- -- -- *-- --Streblospio -- -- -- -- -- -- -- -- benedicti -- -- -- -- * * -- * -- * * * -- * * * * * -- * * -- * Arthropoda Insecta Coleoptera Hydrophilidae Berosus sp. -- -- -- -- * -- -- -- -- -- -- -- * -- -- -- -- -- -- -- -- -- -- -- -- * -- * -- -- Haliplidae Ablabesmyia sp. ✓ -- ✓ -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Peltodytessp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- * -- -- -- -- Diptera Ceratopogonidae Bezzia/Palpomyia complex -- -- -- -- -- * * -- -- -- * -- * -- * * * * * * Chaoboridae Chaoborus puctipennis ✓ -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Chironomidae Chironomus decorus * * * -- * * * * * * * * * * * * * * * * * -- * * -- -- * * * * 1-42 Table I-34. (continuedl. Taxonomic hierarchy Broomfield Creek SCUT1 (control) Jacks Creek Little Creek (control) Jacobs Creek PA2 (control) Drinkwater Creek Long Creek (control) Tooley Creek Muddy Creek (control) Huddles Cut Porter Creek DCUT11 DCUT19 (control) Duck Creek (control) Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Up Down Chironomus stigmaterus -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- ✓ -- -- -- Chironomus sp. -- -- -- -- -- -- -- -- -- ---- -- -- -- -- -- *-- -- -- -- --Coelotanypus -- -- -- -- -- -- -- -- sp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- * Cricotopus sp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- * * -- -- -- -- Cryptochironomus sp. * * * * -- -- -- -- -- -- -- -- -- -- -- -- ---- -- -- -- -- -- -- --- -- -- -- -- -- Dicrotendipes nervosus -- -- -- -- -- * * * ---- -- -- -- -- -- -- --Endochironomus * -- * * * * * -- sp. -- -- -- -- -- -- * -- -- -- -- -- * -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Goeldichironomus devineyae -- -- -- -- -- -- * -- -- * * * * -- -- * -- -- -- -- -- -- -- -- -- -- -- Kiefferulus sp. -- -- ✓ -- ✓ -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Nanocladius sp. -- -- -- -- -- -- -- -- * -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- * * * * -- -- Orthocladiinae sp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- ✓ -- -- -- -- Parachironomus sp. -- -- -- -- -- -- * -- -- * * * -- -- -- -- -- -- -- -- ---- -- -- * * ---- -- -- Parakiefferiella coronata -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- ✓ -- -- -- Polypedilum sp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- * -- * -- * *-- --Polypedilum -- -- halterale ✓ -- ✓ -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Polypedilum tritum -- -- * -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Procladius sp. -- -- * * -- * -- -- -- * -- * -- * * -- -- -- -- * -- * * * * * Tanypus neopunctipennis * * * * * * -- * * * * * * * * * * * * * * -- * -- -- * * -- * * Tanytarsus sp. -- * * -- * -- -- -- * -- * * * -- -- -- * -- * * * * * -- * * * * * * Tribelos jucundus ✓ -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Ephydridaesp. -- -- ✓ -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Tabanidae Chrysopssp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Hemiptera Belostomatidae Abedus/Belostoma sp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Corixidae Corixidae sp. -- -- -- -- -- -- -- * -- * -- -- -- -- -- -- -- ---- -- -- -- * -- ------ -- Trichocorixa kanza -- ✓ -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Odonata Coenagrionidae Enallagmasp. -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- * Libellulidae Perithemis tenera -- -- ✓ -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- Brachymesia furcate -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- ✓ -- -- -- -- Malacostraca Amphipoda Corophiidae Apocorophium lacustre -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- * -- * -- -- Apocorophium louisianum -- -- -- -- -- -- -- -- -- -- -- * * -- -- -- -- -- * -- -- -- * -- -- -- -- Apocorophium sp. -- -- -- -- -- -- -- -- -- -- * * ---- -- -- -- -- ---- * -- -- -- ---- -- Gammaridae Gammarus tigrinus -- * * * * * * * * * * * * -- * * -- * * * -- * * * * * * * * * Decapoda Palaemonidae -- Palaemonetes pugio -- -- -- -- -- -- -- -- -- -- -- -- * -- -- -- -- -- -- -- * -- -- -- -- -- * -- -- Panopeidae Rhithropanopeus harrisii -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- * -- -- Isopoda Anthuridae Cyathura polity -- -- -- -- -- * -- -- -- -- -- * * -- -- -- -- -- -- -- -- -- -- -- -- -- -- * -- -- Sphaeromatidae Cassidinidea ovalis -- -- -- -- -- -- -- -- -- -- -- -- ✓ -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- ✓ -- 1-43 Table 1-34. (concluded). Taxonomic hierarchy s'»»mnem Creek ocuT1 (control) Jacks Creek Little Creek (»»nu»U Jacobs Creek pAo (control) onnxwute' Creek Long Creek (control) Toolevcnex Muddy Creek (control) Huddles Cut Porter Creek ocuT11 ocuT19 (control) Duck Creek (»»nu»U Up Down up Down up Down un Down up Down up Down up Down up Down un Down up Down up Down un Down up Down up Down up Down mmemue sdooamonmsa Myumu Myumue Amoricanpsisalmyra *argem,rapax mutrucomu cunuonmue oanuonmaeep. Cythenuemue o)mxm,isxmrraxs wm|myxu s|vu|v|u e|vawaep. om|ysenmue Mytilopms leunopxaoam Macmuae Mulinia lamraxs Temnmue mmnomammm masm,mmu suuommumnhom Phvemaeep. (pxysalpxysoxa) nyummmue Lomxmnopsep. "-" = Species not collected at station Table 1-35. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Broomfield Creek upstream station in May 2021. Species/taxon Broomfield Creek Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 26 24 32 34 24 28.0 Limnodrilus hoffmeistreri 4 4 6 0 10 4.8 Arthropoda Insecta Coleoptera sp. Ablabesmyia sp. 0 0 2 0 0 0.4 Diptera Chaoborus punctipennis 2 0 0 0 0 0.4 Chironomus decorus 2 2 4 8 13 5.8 Cryptochironomus sp. 6 4 0 14 1 5.0 Dicrotendipes nervosus 2 0 0 0 0 0.4 Polypedilum halterale 2 2 0 0 0 0.8 Procladius sp. 16 2 4 0 7 5.8 Tanypus neopunctipennis 8 4 14 0 3 5.8 Tribelos jucundus 2 2 0 2 1 1.4 Hemiptera Corixidae sp. 0 0 0 2 0 0.4 Mysida Americamysis almyra 2 0 0 0 0 0.4 Total taxa by sample 72 44 62 60 59 Total abundance = 297 Total taxa present = 12 Shannon -Wiener diversity = 1.76 1-45 Table 1-36. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Broomfield Creek upstream station in May 2021. Species/taxona Number of individuals" Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Limnodrilus hoffmeistreri 24 1.0 10 10.0 Naididae sp. w/o hair 140 1.0 100 100.0 Arthropoda Insecta Diptera Chaoborus punctipennis 2 1.4 1 1.4 Chironomus decorus 29 1.0 10 10.0 Cryptochironomus sp. 25 1.0 10 10.0 Dicrotendipes nervosus 2 1.0 1 1.0 Procladius sp. 29 1.2 10 12.0 Tanypus neopunctipennis 29 1.0 10 10.0 Hemiptera Corixidae sp. 2 1.3 1 1.3 Mysida Americamysis almyra 2 2.2 1 2.2 Total 154 157.9 Estuarine Biotic Index = 1.03 Total taxa = 12 Total taxa with sensitivity values = 9 a Only those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. 1-46 Table 1-37. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Broomfield Creek downstream station in May 2021. Species/taxon Broomfield Creek Downstream Samples 1 2 3 4 5 Average Annelida CA -, O O O O O O O -, O GJ C7) 0 0 0 0 0 0 0 0 0 - - W Clitellata Limnodrilus hoffmeisteri 2 0 2 1.6 Naididae sp. w/o hair 4 5 0 2.6 Polychaeta Amphicteis floridus 1 0 0 0.4 Arthropoda Insecta Diptera Chironomus decorus 1 0 0 0.4 Cryptochironomus sp. 1 0 0 0.2 Dicrotendipes nervosus 2 1 0 0.6 Procladius sp. 1 1 0 0.4 Tanypus neopunctipennis 0 1 1 0.4 Tanytarsus sp. 0 0 1 0.2 Trichocorixa kanza 0 0 1 0.2 Malacostraca Amphipoda Gammarus tigrinus 1 0 0 0.2 Mysida Americamysis almyra 0 0 0 0.2 Total taxa by sample 13 8 5 Total abundance = 37 Total taxa present = 11 Shannon -Wiener diversity = 2.02 1-47 Table 1-38. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Broomfield Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Limnodrilus hoffmeisteri 8 1.0 3 3.0 Naididae sp. w/o hair 13 1.0 10 10.0 Polychaeta Amphicteis floridus 2 2.0 1 2.0 Arthropoda Insecta Diptera Chironomus decorus 2 1.0 1 1.0 Cryptochironomus sp. 1 1.0 1 1.0 Dicrotendipes nervosus 3 1.0 3 3.0 Procladius sp. 2 1.2 1 1.2 Tanypus neopunctipennis 2 1.0 1 1.0 Tanytarsus sp. 1 2.3 1 2.3 Malacostraca Amphipoda Gammarus tigrinus 1 2.0 1 2.0 Mysida Americamysis almyra 1 2.2 1 2.2 Total 24 28.7 Estuarine Biotic Index = 1.20 Total taxa = 11 Total taxa with sensitivity values = 10 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. 1-48 Table 1-39. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the SCUT1 upstream station in May 2021. Species/taxon SCUT1 Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Limnodrilus hoffmeisteri 18 0 2 0 6 5.2 Naididae sp. w/o hair 54 14 4 15 26 22.6 Arthropoda Insecta Coleoptera Ablabesmyia sp. 2 0 0 0 2 0.8 Diptera Bezzia/Palpomyia complex 0 0 2 0 0 0.4 Chironomus decorus 54 12 0 24 5 19.0 Cryptochironomus sp. 6 0 2 0 1 1.8 Dicrotendipes nervosus 0 0 0 6 4 2.0 Ephydridae sp. 0 2 0 0 0 0.4 Kiefferulus sp. 0 0 0 0 2 0.4 Parachironomus sp. 0 0 2 0 1 0.6 Polypedilum halterale 12 2 8 0 6 5.6 Polypedilum tritum 0 0 0 3 0 0.6 Procladius sp. 2 2 2 15 6 5.4 Tanypus neopunctipennis 40 8 4 3 2 11.4 Tanytarsus sp. 0 0 2 0 2 0.8 Odanata Perithemis tenera 0 0 2 0 1 0.6 Malacostraca Amphipoda Gammarus tigrinus 0 0 0 0 1 0.2 Total taxa by sample 188 40 30 66 65 Total abundance = 389 Total taxa present = 16 Shannon -Wiener diversity = 2.02 1-49 Table 1-40. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the SCUT1 upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 113 1.0 100.0 100.0 Limnodrilus hoffmeisteri 26 1.0 10 10.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 2 2.3 1 2.3 Chironomus decorus 95 1.0 30 30.0 Cryptochironomus sp. 9 1.0 3 3.0 Dicrotendipes nervosus 10 1.0 10 10.0 Parachironomus sp. 3 1.1 3 3.3 Polypedilum tritum 3 1.3 3 3.9 Procladius sp. 27 1.2 10 12.0 Tanypus neopunctipennis 57 1.0 30 30.0 Tanytarsus sp. 4 2.3 3 6.9 Odanata Perithemis tenera 3 1.0 3 3.0 Malacostraca Amphipoda Gammarus tigrinus 1 2.0 1 2.0 Total 207 216.4 Estuarine Biotic Index = 1.05 Total taxa present= 16 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-50 Table 1-41. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the SCUT1 downstream station in May 2021. Species/taxon SCUT1 Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 0 0 0 0 2 0.4 Tubificoides heterochaetus 2 0 0 0 2 0.8 Polychaeta Amphicteis floridus 35 21 14 12 20 20.4 Arthropoda Insecta Diptera Cryptochironomus sp. 1 1 0 0 2 0.8 Procladius sp. 2 0 0 0 0 0.4 Tanypus neopunctipennis 1 0 0 0 0 0.2 Malacostraca Amphipoda Gammarus tigrinus 0 0 36 54 66 31.2 Mysida Americamysis almyra 0 0 2 0 0 0.4 Ostracoda Cyprideis littoralis 0 0 0 0 4 0.8 Total taxa by sample 41 22 52 66 96 Total abundance = 277 Total taxa present = 8 Shannon -Wiener diversity = 1.00 1-51 Table 1-42. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the SCUT1 downstream station in Ma v 2021. Species/taxona Number of individuals" Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 2 1.0 1 1.0 Tubificoides heterochaetus 4 1.1 3 3.3 Polychaeta Amphicteis floridus 102 2.0 100 200.0 Arthropoda Insecta Diptera Cryptochironomus sp. 4 1.0 3 3.0 Procladius sp. 2 1.2 1 1.2 Tanypus neopunctipennis 1 1.0 1 1.0 Malacostraca Amphipoda Gammarus tigrinus 156 2.0 100 200.0 Mysida Americamysis almyra 2 2.2 1 2.2 Total 210 411.7 Estuarine Biotic Index = 1.96 Total taxa = 8 Total taxa with sensitivity values = 7 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-52 Table 1-43. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Jacks Creek upstream station in May 2021. Species/taxon Jacks Creek Upstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 2 1 6 0 0 1.8 Laeonereis culveri 7 8 12 2 0 5.8 Streblospio benedicti 1 1 2 0 0 0.8 Arthropoda Insecta Coleoptera Berosus sp. 0 0 1 0 0 0.2 Diptera Bezzia/Palpomyia Complex 0 1 4 0 1 1.2 Chironomus decorus 24 14 24 12 3 15.4 Kiefferulus sp. 1 0 0 0 0 0.2 Tanypus neopunctipennis 16 6 21 2 5 10.0 Tanytarsus sp. 1 0 0 0 0 0.2 Malacostraca Amphipoda Apocorophium sp. 0 1 0 0 0 0.2 Gammarus tigrinus 0 0 1 0 0 0.2 Mysida Americamysis almyra 3 0 0 0 0 0.6 Ostracoda Candonidae sp. 0 1 0 0 0 0.2 Cyprideis littoralis 1 0 1 0 0 0.4 Mollusca Gastropoda Littoridinops sp. 2 0 0 0 0 0.4 Total taxa by sample 58 33 72 16 9 Total abundance = 188 Total taxa present = 15 Shannon -Wiener diversity = 1.67 1-53 Table 1-44. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Jacks Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 9 2.0 3 6.0 Laeonereis culveri 29 1.0 10 10.0 Streblospio benedicti 4 1.1 3 3.3 Arthropoda 1 nsecta Coleoptera Berosus sp. 1 1.5 1 1.5 Di pte ra Bezzia/Palpomyia Complex 6 2.3 3 6.9 Chironomus decorus 77 1.0 30 30.0 Tanypus neopunctipennis 50 1.0 30 30.0 Tanytarsus sp. 1 2.3 1 2.3 Malacostraca Amphipoda Apocorophium sp. 1 2.2 1 2.2 Gammarus tigrinus 1 2.0 1 2.0 Mysida Americamysis almyra 3 2.2 3 6.6 Mollusca Gastropoda Littoridinops sp. 2 2.0 1 2.0 Total 87 102.8 Estuarine Biotic Index = 1.18 Total taxa = 15 Total taxa with sensitivity values = 12 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. 1-54 Table 1-45. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Jacks Creek downstream station in May 2021. Species/taxon Jacks Creek Downstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 5 1 10 0 0 3.2 Laeonereis culveri 2 1 2 5 2 2.4 Streblospio benedicti 0 0 7 12 0 3.8 Arthropoda Insecta D i pte ra Chironomus decorus 100 92 100 94 81 93.4 Tanypus neopunctipennis 2 2 2 3 1 2.0 Malacostraca Amphipoda Gammarus tigrinus 3 3 3 0 1 2.0 Isopoda Cyathura polita 0 0 0 0 1 0.2 Mysida Americamysis almyra 0 0 1 0 1 0.4 Ostracoda Cyprideis littoralis 1 0 1 1 3 1.2 Mollusca Gastropoda Littoridinops sp. 9 2 4 2 4 4.2 Total taxa by sample 122 101 130 117 94 Total abundance = 564 Total taxa present = 10 Shannon -Wiener diversity = 0.80 1-55 Table 1-46. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Jacks Creek downstream station in Ma v 2021. Species/taxona Number of individuals" Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 16 2.0 10 20.0 Laeonereis culveri 12 1.0 10 10.0 Streblospio benedicti 19 1.1 10 11.0 Arthropoda Insecta Diptera Chironomus decorus 467 1.0 100 100.0 Tanypus neopunctipennis 10 1.0 10 10.0 Malacostraca Amphipoda Gammarus tigrinus 10 2.0 10 20.0 Isopoda Cyathura polita 1 2.0 1 2.0 Mysida Americamysis almyra 2 2.2 1 2.2 Gastropoda Littoridinops sp. 21 2.0 10 20.0 Total 162 195.2 Estuarine Biotic Index = 1.20 Total taxa = 10 Total taxa with sensitivity values = 9 a Only those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-56 Table 1-47. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Little Creek upstream station in May 2021. Little Creek Upstream Samples Species/taxon 1 2 3 4 5 Average Annelida Clitellata Limnodrilus hoffmeisteri 8 4 12 2 2 5.6 Polychaeta Amphicteis floridus 50 34 78 12 28 40.4 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 0 0 2 0 2 0.8 Chironomus decorus 70 32 74 50 26 50.4 Dicrotendipes nervosus 0 0 6 0 0 1.2 Endochironomus sp. 0 0 4 0 0 0.8 Goeldichironomus devineyae 0 0 2 0 0 0.4 Parachironomus sp. 0 0 0 2 0 0.4 Procladius sp. 6 0 4 0 2 2.4 Hemiptera Corixidae sp. 0 0 0 0 2 0.4 Malacostraca Amphipoda Gammarus tigrinus 0 0 6 4 0 2.0 Ostracoda Cyprideis littoralis 2 0 0 0 0 0.4 Mollusca Bivalvia Mytilopsis leucophaeata 0 0 0 0 2 0.4 Gastropoda Littoridinops sp. 0 0 0 4 0 0.8 Physidae sp. (physalphysella) 0 0 2 0 0 0.4 Total taxa by sample 136 70 190 74 64 Total abundance = 534 Total taxa present = 15 Shannon -Wiener diversity = 1.32 1-57 Table 1-48. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Little Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Limnodrilus hoffmeisteri 28 1.0 10 10.0 Polychaeta Amphicteis floridus 202 2.0 100 200.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 4 2.3 3 6.9 Chironomus decorus 252 1.0 100 100.0 Dicrotendipes nervosus 6 1.0 3 3.0 Endochironomus sp. 4 1.1 3 3.3 Goeldichironomus devineyae 2 2.0 1 2.0 Parachironomus sp. 2 1.1 1 1.1 Procladius sp. 12 1.2 10 12.0 Hemiptera Corixidae sp. 2 1.3 1 1.3 Malacostraca Amphipoda Gammarus tigrinus 10 2.0 10 20.0 Mollusca Bivalvia Mytilopsis leucophaeata 2 1.5 1 1.5 Gastropoda Littoridinops sp. 4 2.0 3 6.0 Physidae sp. (physalphysella) 2 1.3 1 1.3 Total 247 368.4 Estuarine Biotic Index = 1.49 Total taxa = 15 Total taxa with sensitivity values = 14 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-58 Table 1-49. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Little Creek downstream station in May of 2021. Species/taxon Little Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 0 0 0 1 0 0.2 Polychaeta Laeonereis culveri 0 1 0 1 0 0.4 Streblospio benedicti 1 2 1 2 7 2.6 Arthropoda Insecta Diptera Bezzia/Palpomyia Complex 0 0 0 0 1 0.2 Chironomus decorus 100 100 100 100 100 100.0 Procladius sp. 1 0 2 0 0 0.6 Tanypus neopunctipennis 1 1 3 4 5 2.8 Malacostraca Amphipoda Gammarus tigrinus 0 0 1 0 0 0.2 Mysida Americamysis almyra 0 1 0 0 0 0.2 Mollusca Bivalvia Mulinia lateralis 0 0 1 1 0 0.4 Gastropoda Littoridinops sp. 0 0 0 1 0 0.2 Total taxa by sample 103 105 108 110 113 Total abundance = 539 Total taxa present = 11 Shannon -Wiener diversity = 0.38 1-59 Table 1-50. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Little Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 1 1.0 1 1.0 Polychaeta Laeonereis culveri 2 1.0 1 1.0 Streblospio benedicti 13 1.1 10 11.0 Arthropoda Insecta Diptera Bezzia/Palpomyia Complex 1 2.3 1 2.3 Chironomus decorus 500 1.0 100 100.0 Procladius sp. 3 1.2 3 3.6 Tanypus neopunctipennis 14 1.0 10 10.0 Malacostraca Amphipoda Gammarus tigrinus 1 2.0 1 2.0 Mysida Americamysis almyra 1 2.2 1 2.2 Mollusca Bivalvia Mulinia lateralis 2 2.3 1 2.3 Gastropoda Littoridinops sp. 1 2.0 1 2.0 Total 130 137.4 Estuarine Biotic Index = 1.06 Total taxa = 11 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. 1-60 Table 1-51. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Jacobs Creek upstream station in May 2021. Species/taxon Jacobs Creek Upstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis tloridus 0 0 1 0 0 0.2 Laeonereis culveri 3 0 2 9 5 3.8 Arthropoda I nsecta Diptera Bezzia/Palpomyia Complex 0 0 0 1 3 0.8 Chironomus decorus 19 38 37 1 9 20.8 Chrysops sp. 0 0 0 2 0 0.4 Nanocladius sp. 0 0 1 0 0 0.2 Parachironomus sp. 0 0 5 0 0 1.0 Tanypus neopunctipennis 5 6 3 7 1 4.4 Tanytarsus sp. 1 1 0 0 0 0.4 Malacostraca Amphipoda Gammarus tigrinus 0 0 4 2 0 1.2 Mysida Americamysis almyra 1 0 0 0 2 0.6 Mollusca Gastropoda Littoridinops sp. 2 1 2 0 0 1.0 Total taxa by sample 31 46 55 22 20 Total abundance = 174 Total taxa present = 12 Shannon -Wiener diversity = 1.45 1-61 Table 1-52. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Jacobs Creek upstream station in Ma v 2021. Species/taxona Number of individuals" Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 1 2.0 1 2.0 Laeonereis culveri 19 1.0 10 10.0 Arthropoda 1 nsecta Diptera Bezzia/Palpomyia Complex 4 2.3 3 6.9 Chironomus decorus 104 1.0 100 100.0 Chrysops sp. 2 2.2 1 2.2 Nanocladius sp. 1 2.0 1 2.0 Parachironomus sp. 5 1.1 3 3.3 Tanypus neopunctipennis 22 1.0 10 10.0 Tanytarsus sp. 2 2.3 1 2.3 Mysida Americamysis almyra 3 2.2 3 6.6 Malacostraca Amphipoda Gammarus tigrinus 6 2.0 3 6.0 Mollusca Gastropoda Littoridinops sp. 5 2.0 3 6.0 Total 139 157.3 Estuarine Biotic Index = 1.13 Total taxa = 12 Total taxa with sensitivity values = 12 a Only those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all five ponar replicates. 'Sensitivity values provided by NCDWR. 1-62 Table 1-53. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Jacobs Creek downstream station in May 2021. Species/taxon Jacobs Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Tubificoides heterochaetus 1 0 2 0 0 0.6 Polychaeta Amphicteis floridus 1 0 1 0 0 0.4 Heteromastus filiformis 3 0 1 0 0 0.8 Marenzelleria viridis 1 0 0 0 0 0.2 Mediomastus ambiseta 0 0 1 0 0 0.2 Streblospio benedicti 0 1 12 3 1 3.4 Arthropoda Insecta Diptera Chironomus decorus 100 68 100 91 71 86.0 Goeldichironomus devineyae 0 0 1 0 0 0.2 Procladius sp. 0 0 1 0 0 0.2 Tanypus neopunctipennis 3 0 2 0 3 1.6 Malacostraca Amphipoda Gammarus tigrinus 0 0 1 0 0 0.2 Isopoda Edotia montosa 0 1 0 0 0 0.2 Mysida Americamysis almyra 2 4 1 1 0 1.6 Mollusca Bivalvia Macoma tenta 1 0 0 0 0 0.2 Mulinia lateralis 1 0 0 0 0 0.2 Total taxa by sample 113 74 123 95 75 Total abundance = 480 Total taxa present = 15 Shannon -Wiener diversity = 0.47 1-63 Table 1-54. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Jacobs Creek downstream station in May 2021. Species/taxona Number of individuals" Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Clitellata Tubificoides heterochaetus 3 1.1 3 3.3 Polychaeta Amphicteis tloridus 2 2.0 1 2.0 Heteromastus filiformis 4 1.1 3 3.3 Marenzelleria viridis 1 2.6 1 2.6 Mediomastus ambiseta 1 2.0 1 2.0 Streblospio benedicti 17 1.1 10 11.0 Arthropoda Insecta Diptera Chironomus decorus 430 1.0 100 100.0 Goeldichironomus devineyae 1 2.0 1 2.0 Procladius sp. 1 1.2 1 1.2 Tanypus neopunctipennis 8 1.0 3 3.0 Malacostraca Amphipoda Gammarus tigrinus 1 2.0 1 2.0 Isopoda Edotia montosa 1 2.6 1 2.6 Mysida Americamysis almyra 8 2.2 3 6.6 Mollusca Bivalvia Macoma tenta 1 2.0 1 2.0 Mulinia lateralis 1 2.3 1 2.3 Total 131 145.9 Estuarine Biotic Index = 1.11 Total taxa = 15 Total taxa with sensitivity values = 15 a Only those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all five ponar replicates. 'Sensitivity values provided by NCDWR. 1-64 Table 1-55. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the PA2 upstream station in May 2021. Species/taxon PA2 Creek Upstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 3 3 1 1 5 2.6 Laeonereis culveri 10 6 4 4 4 5.6 Streblospio benedicti 0 1 0 0 1 0.4 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 0 0 2 1 3 1.2 Chironomus decorus 54 55 58 38 39 48.8 Dicrotendipes nervosus 0 1 0 0 0 0.2 Goeldichironomus devineyae 1 0 0 0 0 0.2 Parachironomus sp. 1 0 1 2 0 0.8 Tanypus neopunctipennis 10 9 4 3 10 7.2 Tanytarsus sp. 0 0 1 0 0 0.2 Hemiptera Corixidae sp. 5 1 2 4 0 2.4 Malacostraca Amphipoda Gammarus tigrinus 0 2 3 2 0 1.4 Ostracoda Cyprideis littoralis 0 0 1 0 0 0.2 Mollusca Gastropoda Littoridinops sp. 1 2 8 5 2 3.6 Total taxa by sample 85 80 85 60 64 Total abundance = 374 Total taxa present = 14 Shannon -Wiener diversity = 1.35 1-65 Table 1-56. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the PA2 downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 13 2.0 10 20.0 Laeonereis culveri 28 1.0 10 10.0 Streblospio benedecti 2 1.1 1 1.1 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 6 2.3 3 6.9 Chironomus decorus 244 1.0 100 100.0 Dicrotendipes nervosus 1 1.0 1 1.0 Goeldichironomus devineyae 1 2.0 1 2.0 Parachironomus sp. 4 1.1 3 3.3 Tanypus neopunctipennis 36 1.0 30 30.0 Tanytarsus sp. 1 2.3 1 2.3 Hemiptera Corixidae sp. 12 1.3 10 13.0 Malacostraca Amphipoda Gammarus tigrinus 7 2.0 3 6.0 Mollusca Gastropoda Littoridinops sp. 18 2.0 10 20.0 Total 183 215.6 Estuarine Biotic Index = 1.18 Total taxa = 14 Total taxa with sensitivity values = 13 1-66 Table 1-57. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the PA2 downstream station in May 2021. Species/taxon PA2 Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/ hair 0 5 2 0 0 1.4 Naididae sp. w/o hair 0 1 0 0 0 0.2 Polychaeta Amphicteis floridus 43 52 42 33 10 36.0 Laeonereis culveri 51 53 43 29 11 37.4 Streblospio benedecti 2 9 16 1 0 5.6 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 3 1 0 0 1 1.0 Chironomus decorus 34 30 18 30 26 27.6 Dicrotendipes nervosus 4 7 2 4 0 3.4 Goeldichironomus devineyae 0 1 0 0 0 0.2 Parachironomus sp. 4 5 1 13 0 4.6 Polypedilum sp. 0 0 2 0 0 0.4 Tanypus neopunctipennis 4 1 0 0 2 1.4 Tanytarsus sp. 17 26 3 12 1 11.8 Malacostraca Amphipoda Apocorophium louisianum 0 0 0 1 0 0.2 Apocorophium sp. 0 2 0 2 0 0.8 Gammarus tigrinus 6 8 11 2 0 5.4 Isopoda Cyathura polita 0 0 2 0 0 0.4 Edotia montosa 0 1 0 0 0 0.2 Mysida Americamysis almyra 0 0 8 0 0 1.6 Ostracoda Candonidae sp. 1 0 0 0 0 0.2 Cyprideis littoralis 0 1 0 0 0 0.2 Mollusca Gastropoda Littoridinops sp. 31 10 14 5 2 12.4 Total taxa by sample 200 213 164 132 53 Total abundance = 762 Total taxa present = 21 Shannon -Wiener diversity = 2.11 1-67 Table 1-58. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the PA2 downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/ hair 7 1.0 3 3.0 Naididae sp. w/o hair 1 1.0 1 1.0 Polychaeta Amphicteis floridus 180 2.0 100 200.0 Laeonereis culveri 187 1.0 100 100.0 Streblospio benedecti 28 1.1 10 11.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 5 2.3 3 6.9 Chironomus decorus 138 1.0 100 100.0 Dicrotendipes nervosus 17 1.0 10 10.0 Goeldichironomus devineyae 1 2.0 1 2.0 Parachironomus sp. 23 1.1 10 11.0 Polypedilum sp. 2 1.5 1 1.5 Tanypus neopunctipennis 7 1.0 3 3.0 Tanytarsus sp. 59 2.3 30 69.0 Malacostraca Amphipoda Apocorophium louisianum 1 2.2 1 2.2 Apocorophium sp. 4 2.2 3 6.6 Gammarus tigrinus 27 2.0 10 20.0 Isopoda Cyathura polita 2 2.0 1 2.0 Edotia montosa 1 2.6 1 2.6 Mysida Americamysis almya 8 2.2 3 6.6 Mollusca Gastropoda Littoridinops sp. 62 2.0 30 60.0 Total 421 618.4 Estuarine Biotic Index = 1.47 Total taxa = 21 Total taxa with sensitivity values = 19 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-68 Table 1-59. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Drinkwater Creek upstream station in May 2021. Species/taxon Drinkwater Creek Upstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 14 2 0 5 0 4.2 Laeonereis culveri 5 1 0 1 1 1.6 Arthropoda Insecta Coleoptera sp. Berosus sp. 1 0 0 0 0 0.2 Diptera Bezzia/Palpomyia Complex 2 1 0 1 1 1.0 Chironomus decorus 19 14 23 5 14 15.0 Dicrotendipes nervosus 4 8 4 6 2 4.8 Endochironomus sp. 1 0 0 0 0 0.2 Goeldichironomus devineyae 0 0 0 1 0 0.2 Parachironomus sp. 0 0 0 0 1 0.2 Tanypus neopunctipennis 1 2 1 1 0 1.0 Tanytarsus sp. 1 0 3 0 5 1.8 Hemiptera Corixidae sp. 0 1 2 0 1 0.8 Malacostraca Amphipoda Apocorophium louisianum 2 0 0 0 0 0.4 Apocorophium sp. 2 1 0 1 0 0.8 Gammarus tigrinus 24 19 37 27 57 32.8 Decapoda Palaemonetes pugio 0 0 0 1 0 0.2 Isopoda Cassidinidea lunifrons 1 0 0 0 0 0.2 Cyathura polita 1 0 0 0 0 0.2 Mysida Americamysis almyra 0 3 0 0 1 0.8 Ostracoda Candonidae sp. 1 0 1 0 0 0.4 Mollusca Gastropoda Littoridinops sp. 0 3 3 1 2 1.8 Total taxa by sample 79 55 74 50 85 Total abundance = 343 Total taxa present = 20 Shannon -Wiener diversity = 1.78 1-69 Table 1-60. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Drinkwater Creek upstream station in May 2021. Species/taxona Number of individuals" Sensitivity value (SV)` Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis tloridus 21 2.0 10 20.0 Laeonereis culveri 8 1.0 3 3.0 Arthropoda Insecta Coleoptera sp. Berosus sp. 1 1.5 1 1.5 Diptera Bezzia/Palpomyia Complex 5 2.3 3 6.9 Chironomus decorus 75 1.0 30 30.0 Dicrotendipes nervosus 24 1.0 10 10.0 Endochironomus sp. 1 1.1 1 1.1 Goeldichironomus devineyae 1 2.0 1 2.0 Parachironomus sp. 1 1.1 1 1.1 Tanypus neopunctipennis 5 1.0 3 3.0 Tanytarsus sp. 9 2.3 3 6.9 Hemiptera Corixidae sp. 4 1.3 3 3.9 Malacostraca Amphipoda Apocorophium louisianum 2 2.2 1 2.2 Apocorophium sp. 4 2.2 3 6.6 Gammarus tigrinus 164 2.0 100 200.0 Decapoda Palaemonetes pugio 1 2.5 1 2.5 Isopoda Cyathura polita 1 2.0 1 2.0 Mysida Americamysis almyra 4 2.2 3 6.6 Mollusca Gastropoda Littoridinops sp. 9 2.0 3 6.0 Total 181 315.3 Estuarine Biotic Index = 1.74 Total taxa = 20 Total taxa with sensitivity values = 18 a Only those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. 1-70 Table 1-61. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Drinkwater Creek downstream station in May 2021. Species/taxon Drinkwater Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Tubificoides heterochaetus 2 0 1 0 0 0.6 Polychaeta Amphicteis floridus 0 1 0 0 1 0.4 Laeonereis culveri 0 0 0 0 2 0.4 Mediomastus ambiseta 3 0 3 0 0 1.2 Streblospio benedicti 11 1 10 5 3 6.0 Arthropoda 1 nsecta Diptera Chironomus decorus 100 58 100 87 100 89.0 Procladius sp. 3 1 1 1 4 2.0 Tanypus neopunctipennis 1 0 0 0 2 0.6 Mysida Americamysis almyra 0 0 0 0 1 0.2 Ostracoda Cyprideis littoralis 1 0 0 0 0 0.2 Mollusca Bivalvia Mulinia lateralis 0 0 0 1 0 0.2 Gastropoda Littoridinops sp. 2 1 0 1 0 0.8 Total taxa by sample 123 62 115 95 113 Total abundance = 508 Total taxa present = 12 Shannon -Wiener diversity = 0.59 1-71 Table 1-62. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Drinkwater Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Tubificoides heterochaetus 3 1.1 3 3.3 Polychaeta Amphicteis floridus 2 2.0 1 2.0 Laeonereis culveri 2 1.0 1 1.0 Streblospio benedicti 30 1.1 30 33.0 Arthropoda Insecta Diptera Chironomus decorus 445 1.0 100 100.0 Mediomastus ambiseta 6 2.0 3 6.0 Procladius sp. 10 1.2 10 12.0 Tanypus neopunctipennis 3 1.0 3 3.0 Mysida Americamysis almyra 1 2.2 1 2.2 Mollusca Bivalvia Mulinia lateralis 1 2.3 1 2.3 Gastropoda Littoridinops sp. 4 2.0 3 6.0 Total 156 170.8 Estuarine Biotic Index = 1.09 Total taxa = 12 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. 1-72 Table 1-63. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Long Creek upstream station in May 2021. Species/taxon Long Creek Upstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 1 1 5 3 2 2.4 Streblospio benedicti 3 1 1 1 1 1.4 Arthropoda Insecta Diptera Chironomus decorus 100 60 97 100 100 91.4 Tanypus neopunctipennis 5 2 1 0 7 3.0 Malacostraca Amphipoda Gammarus tigrinus 2 3 1 4 0 2.0 Mysida Americamysis almyra 1 0 2 1 0 0.8 Ostracoda Cyprideis littoralis 1 1 1 0 0 0.6 Mollusca Gastropoda Littoridinops sp. 0 0 1 1 0 0.4 Total taxa by sample 113 68 109 110 110 Total abundance = 510 Total taxa present = 8 Shannon -Wiener diversity = 0.52 1-73 Table 1-64. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Long Creek upstream station in Ma v 2021. Species/taxona Number of individuals" Sensitivity value (SV)° Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 12 2.0 10 20.0 Streblospio benedicti 7 1.1 3 3.3 Arthropoda Insecta Diptera Chironomus decorus 457 1.0 100 100.0 Tanypus neopunctipennis 15 1.0 10 10.0 Malacostraca Amphipoda Gammarus tigrinus 10 2.0 10 20.0 Mysida Americamysis almyra 4 2.2 3 6.6 Mollusca Gastropoda Littoridinops sp. 2 2.0 1 2.0 Total 137 161.9 Estuarine Biotic Index = 1.18 Total taxa = 8 Total taxa with sensitivity values = 7 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-74 Table 1-65. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Long Creek downstream station in May 2021. Species/taxon Long Creek Downstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 0 0 1 0 0 0.2 Streblospio benedicti 1 4 24 17 1 9.4 Arthropoda Insecta Diptera Bezzia/Palpomyia Complex 0 0 0 0 1 0.2 Chironomus decorus 70 72 100 100 100 88.4 Goeldichironomus devineyae 1 0 0 0 0 0.2 Procladius sp. 0 1 0 0 0 0.2 Tanypus neopunctipennis 0 1 5 4 4 2.8 Malacostraca Amphipoda Gammarus tigrinus 0 0 0 1 0 0.2 Mollusca Bivalvia Macoma tenta 0 0 0 0 1 0.2 Gastropoda Littoridinops sp. 1 0 1 0 0 0.4 Total taxa by sample 73 78 131 122 107 Total abundance = 511 Total taxa present = 10 Shannon -Wiener diversity = 0.54 1-75 Table 1-66. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Long Creek downstream station in Ma v 2021. Species/taxona Number of individuals" Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 1 2.0 1 2.0 Streblospio benedicti 47 1.1 30 33.0 Arthropoda Insecta Diptera Bezzia/Palpomyia Complex 1 2.3 1 2.3 Chironomus decorus 442 1.0 100 100.0 Goeldichironomus devineyae 1 2.0 1 2.0 Procladius sp. 1 1.2 1 1.2 Tanypus neopunctipennis 14 1.0 10 10.0 Malacostraca Amphipoda Gammarus tigrinus 1 2.0 1 2.0 Mollusca Bivalvia Macoma tenta 1 2.0 1 2.0 Gastropoda Littoridinops sp. 2 2.0 1 2.0 Total 147 156.5 Estuarine Biotic Index = 1.06 Total taxa = 10 Total taxa with sensitivity values = 10 a Only those taxa for which a Sensitivity value is available are displayed. bTotal number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-76 Table 1-67. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Tooley Creek upstream station in May 2021. Species/taxon Tooley Creek Upstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 1 0 0 1 0 0.4 Polychaeta Amphicteis floridus 1 2 1 2 0 1.2 Laeonereis culveri 0 1 0 1 0 0.4 Marenzelleria viridis 1 1 0 0 0 0.4 Streblospio benedicti 1 6 1 4 2 2.8 Arthropoda Insecta Diptera Bezzia/Palmonyia complex 0 0 1 0 0 0.2 Chironomus sp. 0 2 0 0 0 0.4 Chironomus decorus 26 29 61 41 71 45.6 Tanypus neopunctipennis 0 1 2 7 1 2.2 Tanytarsus sp. 3 0 1 2 0 1.2 I sopoda Edotia montosa 3 0 0 0 1 0.8 Mysida Americamysis almyra 1 9 10 0 2 4.4 Hargeria rapax 1 0 1 0 0 0.4 Ostracoda Cyprideis littoralis 1 1 0 0 1 0.6 Mollusca Gastropoda Littoridinops sp. 3 1 0 5 2 2.2 Total taxa by sample 42 53 78 63 80 Total abundance = 316 Total taxa present = 14 Shannon -Wiener diversity = 1.22 1-77 Table 1-68. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Tooley Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 2 1.0 1 1.0 Polychaeta Amphicteis floridus 6 2.0 3 6.0 Laeonereis culveri 2 1.0 1 1.0 Marenzelleria viridis 2 2.6 1 2.6 Streblospio benedicti 14 1.1 10 11.0 Arthropoda Insecta Diptera Bezzia/Palmonyia complex 1 2.3 1 2.3 Chironomus sp. 2 1.0 1 1.0 Chironomus decorus 228 1.0 100 100.0 Tanypus neopunctipennis 11 1.0 10 10.0 Tanytarsus sp. 6 2.3 3 6.9 Isopoda Edotia montosa 4 2.6 3 7.8 Mysida Americamysis almyra 22 2.2 10 22.0 Hargeria rapax 2 1.6 1 1.6 Mollusca Gastropoda Littoridinops sp. 11 2.0 10 20.0 Total 155 193.2 Estuarine Biotic Index = 1.25 Total taxa = 14 Total taxa with sensitivity values = 13 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-78 Table 1-69. Numbers of benthic macroinver ebrates collected in each ponar grab sample at the Tooley Creek downstream station in May 2021. Species/taxon Tooley Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Tubificoides heterochaetus 0 1 0 0 0 0.2 Polychaeta Heteromastus filiformis 1 1 1 0 0 0.6 Mediomastus ambiseta 0 1 2 3 1 1.4 Streblospio benedicti 3 5 4 10 18 8.0 Arthropoda I nsecta Diptera Chironomus decorus 100 100 45 100 87 86.4 Procladius sp. 0 0 0 2 0 0.4 Tanypus neopunctipennis 0 0 0 0 1 0.2 Malacostraca Amphipoda Gammarus tigrinus 0 1 0 0 1 0.4 Mysida Americamysis almyra 0 0 0 1 2 0.6 Mollusca Bivalvia Macoma tenta 1 3 3 2 6 3.0 Gastropoda Littoridinops sp. 0 2 0 1 2 1.0 Total taxa by sample 105 114 55 119 118 Total abundance = 511 Total taxa present = 11 Shannon -Wiener diversity = 0.68 1-79 Table 1-70. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Tooley Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Tubificoides heterochaetus 1 1.1 1 1.1 Polychaeta Heteromastus filiformis 3 1.1 3 3.3 Mediomastus ambiseta 7 2.0 3 6.0 Streblospio benedicti 40 1.1 30 33.0 Arthropoda 1 nsecta Diptera Chironomus decorus 432 1.0 100 100.0 Procladius sp. 2 1.2 1 1.2 Tanypus neopunctipennis 1 1.0 1 1.0 Malacostraca Amphipoda Gammarus tigrinus 2 2.0 1 2.0 Mysida Americamysis almyra 3 2.2 3 6.6 Mollusca Bivalvia Macoma tenta 15 2.0 10 20.0 Gastropoda Littoridinops sp. 5 2.0 3 6.0 Total 156 180.2 Estuarine Biotic Index = 1.16 Total taxa = 11 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. Sensitivity values provided by NCDWR. 1-80 Table 1-71. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Muddy Creek upstream station in May 2021. Species/taxon Muddy Creek Upstream Samples 1 2 3 4 5 Average Polychaeta Amphicteis floridus 1 0 3 6 1 2.2 Arthropoda Insecta Diptera Chironomus decorus 50 31 17 62 59 43.8 Procladius sp. 0 0 0 0 1 0.2 Tanypus neopunctipennis 2 2 0 0 1 1.0 Tanytarsus sp. 1 0 0 0 2 0.6 Malacostraca Amphipoda Apocorophium sp. 0 0 2 0 1 0.6 Apocorophium louisianum 0 0 0 4 0 0.8 Gammarus tigrinus 0 1 0 0 2 0.6 Mysida Americamysis almyra 0 0 1 0 0 0.2 Ostracoda Candonidae sp. 0 2 3 0 0 1.0 Cyprideis littoralis 0 0 1 0 0 0.2 Total taxa by sample 54 36 27 72 67 Total abundance = 256 Total taxa present = 10 Shannon -Wiener diversity = 0.71 1-81 Table 1-72. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Muddy Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Polychaeta Amphicteis floridus 11 2 10 20.0 Arthropoda Insecta Diptera Chironomus decorus 219 1.0 100 100.0 Procladius sp. 1 1.2 1 1.2 Tanypus neopunctipennis 5 1.0 3 3.0 Tanytarsus sp. 5 2.3 3 6.9 Malacostraca Amphipoda Apocorophium sp. 3 2.2 3 6.6 Apocorophium louisianum 4 2.2 3 6.6 Gammarus tigrinus 3 2.0 3 6.0 Mysida Americamysis almyra 1 2.2 1 2.2 Total 127 152.5 Estuarine Biotic Index = 1.20 Total taxa = 10 Total taxa with sensitivity values = 8 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-82 Table 1-73. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Muddy Creek downstream station in May 2021. Species/taxon Muddy Creek Downstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 0 2 1 0 0 0.6 Mediomastus ambiseta 0 0 0 0 1 0.2 Neanthes succinea 0 1 0 0 0 0.2 Polydora cornuta 0 1 0 0 0 0.2 Streblospio benedicti 5 2 2 1 4 2.8 Arthropoda Insecta Diptera Chironomus decorus 66 76 89 100 100 86.2 Tanypus neopunctipennis 9 4 6 8 4 6.2 Tanytarsus sp. 0 0 1 0 0 0.2 Malacostraca Amphipoda Gammarus tigrinus 1 1 2 0 0 0.8 Isopoda Edotia montosa 0 1 0 0 0 0.2 Mysida Americamysis almyra 0 3 0 0 0 0.6 Ostracoda Candonidae sp. 0 0 1 0 0 0.2 Mollusca Bivalvia Macoma tenta 0 0 1 0 1 0.4 Gastropoda Littoridinops sp. 0 1 2 1 0 0.8 Total taxa by sample 81 92 105 110 110 Total abundance = 498 Total taxa present = 14 Shannon -Wiener diversity = 0.63 1-83 Table 1-74. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Muddy Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 3 2.0 3 6.0 Mediomastus ambiseta 1 2.0 1 2.0 Neanthes succinea 1 1.7 1 1.7 Polydora cornuta 1 1.2 1 1.2 Streblospio benedicti 14 1.1 10 11.0 Arthropoda Insecta Diptera Chironomus decorus 431 1.0 100 100.0 Tanypus neopunctipennis 31 1.0 30 30.0 Tanytarsus sp. 1 2.3 1 2.3 Malacostraca Amphipoda Gammarus tigrinus 4 2.0 3 6.0 Isopoda Edotia montosa 1 2.6 1 2.6 Mysida Mysidae Americamysis almyra 3 2.2 3 6.6 Mollusca Bivalvia Macoma tents 2 2.0 1 2.0 Gastropoda Littoridinops sp. 4 2.0 3 6.0 Total 158 177.4 Estuarine Biotic Index = 1.12 Total taxa = 14 Total taxa with sensitivity values = 13 a Only those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-84 Table 1-75. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Huddles Cut upstream station in May 2021. Huddles Cut Upstream Samples Species/taxon 1 2 3 4 5 Average Annelida Polychaeta Streblospio benedicti 0 1 0 0 0 0.2 Arthropoda I nsecta Di pte ra Bezzia/Palpomyia Complex 1 2 0 0 1 0.8 Chironomus decorus 33 34 31 47 19 32.8 Polypedilum sp. 2 0 0 0 0 0.4 Tanypus neopunctipennis 2 0 0 1 0 0.6 Tanytarsus sp. 1 0 0 0 0 0.2 Decapoda Palaemonetes pugio 1 0 0 0 0 0.2 Isopoda Edotia montosa 1 0 0 0 0 0.2 Mollusca Bivalvia Macoma tenta 0 0 1 0 0 0.2 Total taxa by sample 41 37 32 48 20 Total abundance = 178 Total taxa present = 9 Shannon -Wiener diversity = 0.43 1-85 Table 1-76. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Huddles Cut upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Streblospio benedicti 1 1.1 1 1.1 Arthropoda Insecta Diptera Bezzia/Palpomyia Complex 4 2.3 3 6.9 Chironomus decorus 164 1.0 100 100.0 Polypedilum sp. 2 1.5 1 1.5 Tanypus neopunctipennis 3 1.0 3 3.0 Tanytarsus sp. 1 2.3 1 2.3 Decapoda Palaemonetes pugio 1 2.5 1 2.5 Isopoda Edotia montosa 1 2.6 1 2.6 Mollusca Bivalvia Macoma tenta 1 2.0 1 2.0 Total 112 121.9 Estuarine Biotic Index = 1.09 Total taxa = 9 Total taxa with sensitivity values = 9 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-86 Table 1-77. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Huddles Cut downstream station in May 2021. Huddles Cut Downstream Samples Species/taxon 1 2 3 4 5 Average Annelida Hirudinea sp. 4 0 0 0 0 0.8 Clitellata Enchytraeidae sp. 0 0 0 2 0 0.4 Naididae sp. w/o hair 46 21 0 4 2 14.6 Polychaeta Laeonereis culvert 0 3 0 0 0 0.6 Marenzelleria viridis 10 6 6 2 2 5.2 Arthropoda I nsecta Diptera Tanytarsus sp. 2 3 0 0 0 1.0 Malacostraca Amphipoda Gammarus tigrinus 0 0 0 0 4 0.8 Mollusca Gastropoda Littoridinops sp. 2 0 0 0 2 0.8 Total taxa by sample 64 33 6 8 10 Total abundance = 121 Total taxa present = 8 Shannon -Wiener diversity = 1.26 1-87 Table 1-78. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Huddles Cut downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Enchytraeidae sp. 2 1 1 1.0 Naididae sp. w/o hair 73 1 30 30.0 Polychaeta Laeonereis culveri 3 2.0 3 6.0 Marenzelleria viridis 26 2.6 10 26.0 Arthropoda I nsecta Diptera Tanytarsus sp. 5 2.3 3 6.9 Malacostraca Amphipoda Gammarus tigrinus 4 2.0 3 6.0 Mollusca Gastropoda Littoridinops sp. 4 2.0 3 6.0 Total 53 81.9 Estuarine Biotic Index = 1.55 Total taxa = 8 Total taxa with sensitivity values = 7 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-88 Table 1-79. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Porter Creek upstream station in May 2021. Species/taxon Porter Creek Upstream Samples 1 2 3 4 5 Average Annelida N N 0 N 0 0 0 0 0 0 0 0 CO Polychaeta Amphicteis floridus 50 6 26 8 19.6 Arthropoda I nsecta Diptera Bezzia/Palpomyia Complex 2 0 0 0 0.4 Chironomus decorus 41 0 4 0 9.0 Dicrotendipes nervosus 0 2 0 0 0.4 Polypedilum sp. 1 0 0 0 0.2 Tanypus neopuntipennis 3 0 0 0 0.6 Tanytarsus sp. 3 2 0 0 1.0 Malacostraca Amphipoda Apocorophium Iouisianum 0 0 4 0 0.8 Apocorophium sp. 0 10 4 2 3.2 Gammarus tigrinus 2 6 10 22 8.4 Ostracoda Candonidae sp. 0 2 10 0 2.4 Mollusca Gastropoda Littoridinops sp. 0 0 0 0 0.4 Total taxa by sample 102 28 58 32 Total abundance = 232 Total taxa present = 11 Shannon -Wiener diversity = 1.68 1-89 Table 1-80. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Porter Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 98 2.0 30 60.0 Arthropoda Insecta Diptera Bezzia/Palpomyia Complex 2 2.3 1 2.3 Chironomus decorus 45 1.0 30 30.0 Dicrotendipes nervosus 2 1.0 1 1.0 Polypedilum sp. 1 1.5 1 1.5 Tanypus neopunctipennis 3 1.0 3 3.0 Tanytarsus sp. 5 2.3 3 6.9 Malacostraca Amphipoda Apocorophium sp. 16 2.2 10 22.0 Apocorophium louisianum 4 2.2 3 6.6 Gammarus tigrinus 42 2.0 30 60.0 Mollusca Gastropoda Littoridinops sp. 2 2.0 1 2.0 Total 113 195.3 Estuarine Biotic Index = 1.73 Total taxa = 11 Total taxa with sensitivity values = 10 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-90 Table 1-81. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Porter Creek downstream station in May 2021. Species/taxon Porter Creek Downstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 0 1 0 0 0 0.2 Mediomastus ambiseta 0 0 2 0 0 0.4 Streblospio benedicti 0 0 4 3 1 1.6 Arthropoda Insecta Diptera Chironomus decorus 82 37 84 58 60 64.2 Proc/adius sp. 0 0 4 1 0 1.0 Malacostraca Amphipoda Gammarus tigrinus 1 0 0 0 0 0.2 Mollusca Bivalvia sp. 1 0 0 0 0 0.2 Ma coma tenta 1 3 3 4 2 2.6 Gastropoda Littoridinops sp. 1 3 0 0 0 0.8 Total taxa by sample 86 44 97 66 63 Total abundance = 356 Total taxa present = 9 Shannon -Wiener diversity = 0.49 1-91 Table 1-82. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Porter Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 1 2.0 1 2.0 Streblospio benedicti 8 1.1 3 3.3 Mediomastus ambiseta 2 2.0 1 2.0 Arthropoda Insecta Diptera Chironomus decorus 321 1.0 100 100.0 Procladius sp. 5 1.2 3 3.6 Malacostraca Amphipoda Gammarus tigrinus 1 2.0 1 2.0 Mollusca Bivalvia Macoma tenta 13 2.0 10 20.0 Gastropoda Littoridinops sp. 4 2.0 3 6.0 Total 122 138.9 Estuarine Biotic Index = 1.14 Total taxa = 9 Total taxa with sensitivity values = 8 a Only those taxa for which a sensitivity value is available are displayed. 1-92 Table 1-83. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the DCUT11 upstream station in May 2021. DCUT11 Upstream Samples Species/taxon 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 0 0 0 0 6 1.2 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 0 3 0 0 0 0.6 Cricotopus sp. 0 0 0 0 8 1.6 Dicrotendipes nervosus 8 12 0 0 6 5.2 Nanocladius sp. 0 9 0 0 0 1.8 Polypedilum sp. 4 0 0 0 0 0.8 Tanytarsus sp. 4 6 0 0 0 2.0 Malacostraca Amphipoda Gammarus tigrinus 0 3 4 0 6 2.6 Mollusca Bivalvia Mytilopsis leucophaeata 0 0 0 0 2 0.4 Gastropoda Littoridinops sp. 0 12 0 9 2 4.6 Total taxa by sample 16 45 4 9 30 Total abundance = 104 Total taxa present = 10 Shannon -Wiener diversity = 2.04 1-93 Table 1-84. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the DCUT11 upstream station in Ma v 2020. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 6 2.0 3 6.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 3 2.3 3 6.9 Cricotopus sp. 8 1.4 3 4.2 Dicrotendipes nervosus 26 1.0 10 10.0 Nanocladius sp. 9 2.0 3 6.0 Polypedilum sp. 4 1.5 3 4.5 Tanytarsus sp. 10 2.3 10 23.0 Malacostraca Amphipoda Gammarus tigrinus 13 2.0 10 20.0 Mollusca Bivalvia Mytilopsis leucophaeata 2 1.5 1 1.5 Gastropoda Littoridinops sp. 23 2.0 10 20.0 Total 56 102.1 Estuarine Biotic Index = 1.82 Total taxa = 10 Total taxa with sensitivity values = 10 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-94 Table 1-85. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the DCUT11 downstream station in May 2021. Species/taxon DCUT11 Downstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 38 20 96 12 52 43.6 Laeonereis culveri 0 0 2 0 0 0.4 Arthropoda Insecta Coleoptera Berosus sp. 0 0 2 0 0 0.4 Peltodytes sp. 0 0 2 0 0 0.4 Diptera Bezzia/Palpomyia complex 0 2 2 2 0 1.2 Cricotopus sp. 2 0 6 8 0 3.2 Dicrotendipes nervosus 28 2 14 42 14 20.0 Nanocladius sp. 0 0 0 4 2 1.2 Orthocladiinae sp. 0 0 0 2 0 0.4 Parachironomus sp. 2 0 0 0 0 0.4 Parakiefferiella coronata 4 2 0 0 0 1.2 Polypedilum sp. 0 0 2 0 0 0.4 Procladius sp. 2 2 0 0 0 0.8 Tanypus neopunctipennis 0 0 0 2 0 0.4 Tanytarsus sp. 16 10 16 18 6 13.2 Hemiptera Corixidae sp. 0 0 2 0 0 0.4 Odonata Brachymesia furcata 0 0 2 0 0 0.4 Malacostraca Amphipoda Apocorophium lacustre 0 0 0 0 2 0.4 Gammarus tigrinus 40 8 14 32 30 24.8 Ostracoda Candonidae sp. 0 2 0 0 0 0.4 Cyprideis littoralis 0 2 0 0 0 0.4 Mollusca Gastropoda Littoridinops sp. 8 10 20 6 10 10.8 Total taxa by sample 140 60 180 128 116 Total abundance = 624 Total taxa present = 22 Shannon -Wiener diversity = 1.91 1-95 Table 1-86. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the DCUT11 downstream station in Ma v 2021. Species/taxona Number of individuals" Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 218 2.0 100 200.0 Laeonereis culveri 2 2.0 1 2.0 Arthropoda Insecta Coleoptera Berosus sp. 2 1.5 1 1.5 Peltodytes sp. 2 1.4 1 1.4 Diptera Bezzia/Palpomyia complex 6 2.3 3 6.9 Cricotopus sp. 16 1.4 10 14.0 Dicrotendipes nervosus 100 1.0 100 100.0 Nanocladius sp. 6 2.0 3 6.0 Parachironomus sp. 2 1.1 1 1.1 Polypedilum sp. 2 1.5 1 1.5 Procladius sp. 4 1.2 3 3.6 Tanypus neopunctipennis 2 1.0 1 1.0 Tanytarsus sp. 66 2.3 30 69.0 Hemiptera Corixidae sp. 2 1.3 1 1.3 Malacostraca Amphipoda Apocorophium lacustre 2 2.2 1 2.2 Gammarus tigrinus 124 2.0 100 200.0 Mollusca Gastropoda Littoridinops sp. 54 2.0 30 60.0 Total 387 671.5 Estuarine Biotic Index = 1.73 Total taxa = 22 Total taxa with sensitivity values = 17 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-96 Table 1-87. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the DCUT19 upstream station in May 2021. Species/taxon DCUT19 Upstream Samples 1 2 3 4 5 Average Annelida Polychaeta Amphicteis floridus 12 0 0 6 3 4.2 Laeonereis culveri 2 0 0 0 0 0.4 Arthropoda I nsecta Diptera Bezzia/Palpomyia complex 0 2 0 9 0 2.2 Chironomus decorus 22 26 96 63 27 46.8 Chironomus stigmaterus 2 0 6 0 0 1.6 Dicrotendipes nervosus 0 0 48 9 0 11.4 Nanocladius sp. 2 2 3 0 0 1.4 Parachironomus sp. 6 2 9 0 0 3.4 Procladius sp. 0 0 3 0 0 0.6 Tanypus neopuntipennis 6 4 15 9 15 9.8 Tanytarsus sp. 18 4 33 15 15 17.0 Malacostraca Amphipoda Gammarus tigrinus 2 0 0 0 0 0.4 Decapoda Palaemonetes pugio 2 0 0 0 0 0.4 Ostracoda Candonidae sp. 14 16 0 57 27 22.8 Mollusca Gastropoda Littoridinops sp. 12 16 18 36 36 23.6 Total taxa by sample 100 72 231 204 123 Total abundance = 730 Total taxa present = 15 Shannon -Wiener diversity = 2.13 1-97 Table 1-88. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the DCUT19 upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Polychaeta Amphicteis floridus 21 2.0 10 20.0 Laeonereis culveri 2 1.0 1 1.0 Arthropoda Insecta Diptera Bezzia/Palpomyia complex 11 2.3 10 23.0 Chironomus decorus 234 1.0 100 100.0 Dicrotendipes nervosus 57 1.0 30 30.0 Nanocladius sp. 7 2.0 3 6.0 Parachironomus sp. 17 1.1 10 11.0 Procladius sp. 3 1.2 3 3.6 Tanypus neopunctipennis 49 1.0 30 30.0 Tanytarsus sp. 85 2.3 30 69.0 Malacostraca Amphipoda Gammarus tigrinus 2 2.0 1 2.0 Decapoda Palaemonetes pugio 2 2.5 1 2.5 Mollusca Gastropoda Littoridinops sp. 118 2.0 100 200.0 Total 329 498.1 Estuarine Biotic Index = 1.63 Total taxa = 15 Total taxa with sensitivity values = 13 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-98 Table 1-89. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the DCUT19 downstream station in May 2021. Species/taxon DCUT19 Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 0 0 28 16 28 14.4 Polychaeta Amphicteis floridus 100 68 100 60 100 85.6 Laeonereis culveri 0 12 4 0 12 5.6 Arthropoda Insecta Coleoptera Berosus sp. 4 4 0 0 0 1.6 Diptera Bezzia/Palpomyia complex 0 0 0 0 4 0.8 Chironomus decorus 0 0 0 0 8 1.6 Dicrotendipes nervosus 40 16 28 44 100 45.6 Goeldichironomus devineyae 0 12 12 16 44 16.8 Nanocladius sp. 0 0 0 0 4 0.8 Procladius sp. 4 0 0 0 4 1.6 Tanytarsus sp. 12 0 4 0 40 11.2 Malacostraca Amphipoda Apocorophium lacustre 0 4 4 0 0 1.6 Apocorophium louisianum 8 0 0 0 0 1.6 Apocorophium sp. 4 0 0 8 0 2.4 Gammarus tigrinus 100 100 100 100 100 100.0 Decapoda Rhithropanopeus harrisii 0 0 0 4 0 0.8 Isopoda Cyathura polita 20 16 12 12 16 15.2 Edotia montosa 4 0 0 0 0 0.8 Mysida Hargeria rapax 100 48 100 80 76 80.8 Ostracoda Candonidae sp. 0 0 24 0 28 10.4 Mollusca Gastropoda Littoridinops sp. 8 12 4 8 12 8.8 Total taxa by sample 404 292 420 348 576 Total abundance = 2040 Total taxa present = 19 Shannon -Wiener diversity = 2.13 1-99 Table 1-90. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the DCUT19 downstream station in Ma v 2021. Species/taxona Number of individuals" Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 72 1.0 30 30.0 Polychaeta Amphicteis floridus 423 2.0 100 200.0 Laeonereis culveri 28 1.0 10 10.0 Arthropoda Insecta Coleoptera Berosus sp. 8 1.5 3 4.5 Diptera Bezzia/Palpomyia complex 4 2.3 3 6.9 Chironomus decorus 8 1.0 3 3.0 Dicrotendipes nervosus 228 1.0 100 100.0 Goeldichironomus devineyae 84 2.0 30 60.0 Nanocladius sp. 4 2.0 3 6.0 Procladius sp. 8 1.2 3 3.6 Tanytarsus sp. 56 2.3 30 69.0 Malacostraca Amphipoda Apocorophium lacustre 8 2.2 3 6.6 Apocorophium louisianum 8 2.2 3 6.6 Apocorophium sp. 12 2.2 10 22.0 Gammarus tigrinus 500 2.0 100 200.0 Decapoda Rhithropanopeus harrisii 4 2.5 3 7.5 Isopoda Cyathura polita 76 2.0 30 60.0 Edotia montosa 4 2.6 3 7.8 Mysida Hargeria rapax 404 1.6 100 160.0 Mollusca Gastropoda Littoridinops sp. 44 2.0 30 60.0 Total 597 1023.5 Estuarine Biotic Index = 1.71 Total taxa = 19 Total taxa with sensitivity values = 18 a Only those taxa for which a sensitivity value is available are displayed. bTotal number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-100 Table 1-91. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Duck Creek upstream station in May 2021. Duck Creek Upstream Samples Species/taxon 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 0 0 0 0 4 0.8 Polychaeta Amphicteis floridus 4 4 12 4 2 5.2 Arthropoda Insecta Diptera Bezzia/Palpomyia Complex 0 0 4 0 4 1.6 Chironomus decorus 4 0 0 6 2 2.4 Dicrotendipes nervosus 4 4 12 2 2 4.8 Procladius sp. 0 0 2 0 2 0.8 Tanypus neopunctipennis 0 0 0 0 2 0.4 Tanytarsus sp. 4 0 0 4 2 2.0 Malacostraca Amphipoda Gammarus tigrinus 0 0 6 2 4 2.4 Isopoda Cassidinidea ovalis 0 0 2 0 0 0.4 Ostracoda Candonidae sp. 4 0 0 2 2 1.6 Mollusca Bivalvia Mytilopsis leucophaeata 0 0 2 0 0 0.4 Gastropoda Littoridinops sp. 0 6 2 0 0 1.6 Total taxa by sample 20 14 42 20 26 Total abundance = 122 Total taxa present = 13 Shannon -Wiener diversity = 2.27 1-101 Table 1-92. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Duck Creek upstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 4 1.0 3 3.0 Polychaeta Amphicteis floridus 26 2.0 10 20.0 Arthropoda Insecta Diptera Bezzia/Palpomyia Complex 8 2.3 3 6.9 Chironomus decorus 12 1.0 10 10.0 Dicrotendipes nervosus 24 1.0 10 10.0 Procladius sp. 4 1.2 3 3.6 Tanypus neopunctipennis 2 1.0 1 1.0 Tanytarsus sp. 10 2.3 10 23.0 Malacostraca Amphipoda Gammarus tigrinus 12 2.0 10 20.0 Mollusca Bivalvia Mytilopsis leucophaeata 2 1.5 1 1.5 Gastropoda Littoridinops sp. 8 2.0 3 6.0 Total 64 105.0 Estuarine Biotic Index = 1.64 Total taxa = 13 Total taxa with sensitivity values = 11 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-102 Table 1-93. Numbers of benthic macroinvertebrates collected in each ponar grab sample at the Duck Creek downstream station in May 2021. Species/taxon Duck Creek Downstream Samples 1 2 3 4 5 Average Annelida Clitellata Naididae sp. w/o hair 0 0 1 0 1 0.4 Polychaeta Amphicteis tloridus 3 1 5 1 1 2.2 Streblospio benedicti 2 0 0 2 0 0.8 Arthropoda Insecta Diptera Bezzia/Palpomyia Complex 0 1 0 0 1 0.4 Chironomus decorus 25 29 34 18 26 26.4 Coelotanypus sp. 0 1 0 0 0 0.2 Procladius sp. 8 2 6 3 11 6.0 Tanypus neopunctipennis 0 0 2 0 1 0.6 Tanytarsus sp. 11 20 18 4 29 16.4 Odonata Enallagma sp. 1 0 0 0 0 0.2 Malacostraca Amphipoda Gammarus tigrinus 0 2 4 1 0 1.4 Mysida Americamysis almyra 0 1 0 0 0 0.2 Ostracoda Cyprideis littoralis 3 0 1 0 0 0.8 Mollusca Gastropoda Littoridinops sp. 2 1 0 0 2 1.0 Total taxa by sample 55 58 71 29 72 Total abundance = 285 Total taxa present = 14 Shannon -Wiener diversity = 1.54 1-103 Table 1-94. Estuarine Biotic Index (EBI) calculations for benthic ponar samples at the Duck Creek downstream station in Ma v 2021. Species/taxona Number of individualsb Sensitivity value (SV)c Abundance value (n) Product of (SV)(n) Annelida Clitellata Naididae sp. w/o hair 2 1 1 1.0 Polychaeta Amphicteis floridus 11 2 10 20.0 Streblospio benedicti 4 1.1 3 3.3 Arthropoda Insecta Diptera Bezzia/Palpomyia Complex 2 2.3 1 2.3 Chironomus decorus 132 1 100 100.0 Coelotanypus sp. 1 1.8 1 1.8 Procladius sp. 30 1.2 30 36.0 Tanypus neopunctipennis 3 1.0 3 3.0 Tanytarsus sp. 82 2.3 30 69.0 Odonata Enallagma sp. 1 1.5 1 1.5 Malacostraca Amphipoda Gammarus tigrinus 7 2.0 3 6.0 Mysida Americamysis almyra 1 2.2 1 2.2 Mollusca Gastropoda Littoridinops sp. 5 2.0 3 6.0 Total 187 252.1 Estuarine Biotic Index = 1.35 Total taxa = 14 Total taxa with sensitivity values = 13 a Only those taxa for which a sensitivity value is available are displayed. b Total number of individuals caught in all five ponar replicates. ° Sensitivity values provided by NCDWR. 1-104 Table 1-95. Hydrographic parameters measured at Broomfield Creek during 18 May 2021 benthic ponar grab and sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 23.2 25.9 23.2 25.9 Dissolved oxygen (%) 95.1 112.2 95.1 112.2 Dissolved oxygen (mg/L) 8.29 9.00 8.29 9.00 Specific conductivity (mS) 0.92 1.72 0.92 1.72 Conductivity (mS) 0.89 1.80 0.89 1.80 Salinity (psu) 0.45 0.87 0.45 0.87 pH 7.12 7.26 7.12 7.26 Secchi depth (inches) 12.00 20.00 12.00 20.00 Near bottom Temperature (°C) 22.0 22.1 22.0 22.1 Dissolved oxygen (%) 76.6 48.3 76.6 48.3 Dissolved oxygen (mg/L) 6.66 4.35 6.66 4.35 Specific conductivity (mS) 0.93 1.52 0.93 1.52 Conductivity (mS) 0.88 1.44 0.88 1.44 Salinity (psu) 0.46 0.77 0.46 0.77 pH 6.99 6.87 6.99 6.87 Table 1-96. Hydrographic parameters measured at SCUT1 during 18 May 2021 benthic ponar grab and sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 21.6 26.4 21.6 26.4 Dissolved oxygen (%) 28.2 115.2 28.2 115.2 Dissolved oxygen (mg/L) 2.47 9.33 2.47 9.33 Specific conductivity (mS) 1.555 1.973 1.555 1.973 Conductivity (mS) 1.455 2.026 1.455 2.026 Salinity (psu) 0.78 1.00 0.78 1.00 pH 7.0 7.33 7.0 7.33 Secchi depth (inches) > 7.5 26.00 > 7.5 26.00 Near bottom Temperature (°C) 21.6 23.9 21.6 23.9 Dissolved oxygen (%) 23.7 83.5 23.7 83.5 Dissolved oxygen (mg/L) 2.08 6.95 2.08 6.95 Specific conductivity (mS) 1.55 1.90 1.55 1.90 Conductivity (mS) 1.45 1.86 1.45 1.86 Salinity (psu) 0.78 0.97 0.78 0.97 pH 6.98 7.03 6.98 7.03 Table 1-97. Hydrographic parameters measured at Jacks Creek during 12 May 2021 benthic ponar grab and sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 19.7 20.1 19.7 20.1 Dissolved oxygen (%) 66.9 82.0 66.9 82.0 Dissolved oxygen (mg/L) 5.96 7.38 5.96 7.38 Specific conductivity (mS) 3.69 3.58 3.69 3.58 Conductivity (mS) 3.31 3.25 3.31 3.25 Salinity (psu) 1.95 1.89 1.95 1.89 pH 6.93 7.45 6.93 7.45 Secchi depth (inches) 24.00 24.00 24.00 24.00 Near bottom Temperature (°C) 19.7 20.2 19.7 20.2 Dissolved oxygen (%) 64.8 78.7 64.8 78.7 Dissolved oxygen (mg/L) 5.81 7.07 5.81 7.07 Specific conductivity (mS) 3.68 3.59 3.68 3.59 Conductivity (mS) 3.31 3.25 3.31 3.25 Salinity (psu) 1.95 1.88 1.95 1.88 pH 6.90 7.45 6.90 7.45 1-107 Table 1-98. Hydrographic parameters measured at Little Creek during 12 May 2021 benthic ponar grab and sweep. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 18.6 19.9 18.6 19.9 Dissolved oxygen (%) 35.4 84.4 35.4 84.4 Dissolved oxygen (mg/L) 3.29 7.55 3.29 7.55 Specific conductivity (mS) 1.21 4.02 1.21 4.02 Conductivity (mS) 1.06 3.63 1.06 3.63 Salinity (psu) 0.60 2.14 0.60 2.14 pH 6.77 7.23 6.77 7.23 Secchi depth (inches) 18.50 20.50 18.50 20.50 Near bottom Temperature (°C) 18.5 19.8 18.5 19.8 Dissolved oxygen (%) 40.2 76.7 40.2 76.7 Dissolved oxygen (mg/L) 3.73 6.86 3.73 6.86 Specific conductivity (mS) 1.28 4.03 1.28 4.03 Conductivity (mS) 1.13 3.63 1.13 3.63 Salinity (psu) 0.64 2.14 0.64 2.14 pH 6.77 7.21 6.77 7.21 1-108 Table 1-99. Hydrographic parameters measured at Jacobs Creek during 13 May 2021 benthic ponar grab and sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 18.0 20.1 18.0 20.1 Dissolved oxygen (%) 75.2 91.5 75.2 91.5 Dissolved oxygen (mg/L) 6.93 8.17 6.93 8.17 Specific conductivity (mS) 4.91 4.70 4.91 4.70 Conductivity (mS) 4.24 4.26 4.24 4.26 Salinity (psu) 2.64 2.52 2.64 2.52 pH 6.91 7.70 6.91 7.70 Secchi depth (inches) >29.00 26.00 >29.00 26.00 Near bottom Temperature (°C) 17.8 18.1 17.8 18.1 Dissolved oxygen (%) 65.6 75.6 65.6 75.6 Dissolved oxygen (mg/L) 6.12 7.04 6.12 7.04 Specific conductivity (mS) 4.85 4.57 4.85 4.57 Conductivity (mS) 4.19 3.97 4.19 3.97 Salinity (psu) 2.61 2.45 2.61 2.45 pH 6.90 7.30 6.90 7.30 1-109 Table 1-100. Hydrographic parameters measured at PA2 during 13 May 2021 benthic ponar grab sampling and benthic sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 16.5 17.9 16.5 17.9 Dissolved oxygen (%) 43.0 63.1 43.0 63.1 Dissolved oxygen (mg/L) 4.11 5.93 4.11 5.93 Specific conductivity (mS) 4.46 4.48 4.46 4.48 Conductivity (mS) 3.73 3.88 3.73 3.88 Salinity (psu) 2.39 2.40 6.69 2.40 pH 6.68 6.83 2.39 6.83 Secchi depth (inches) >33.0 >28.0 >33.0 >28.0 Near bottom Temperature (°C) 16.2 17.2 16.2 17.2 Dissolved oxygen (%) 39.6 63.0 39.6 63.0 Dissolved oxygen (mg/L) 3.84 5.95 3.84 5.95 Specific conductivity (mS) 4.46 4.48 4.46 4.48 Conductivity (mS) 3.71 3.82 3.71 3.82 Salinity (psu) 2.39 2.40 2.39 2.40 pH 6.68 6.83 6.68 6.83 1-110 Table 1-101. Hydrographic parameters measured at Drinkwater Creek during 13 May 2021 benthic ponar grab and sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 14.9 17.6 14.9 17.6 Dissolved oxygen (%) 45.6 81.7 45.6 81.7 Dissolved oxygen (mg/L) 4.54 7.67 4.54 7.67 Specific conductivity (mS) 3.94 4.56 3.94 4.56 Conductivity (mS) 3.18 3.92 3.18 3.92 Salinity (psu) 2.10 2.45 2.10 2.45 pH 6.69 7.19 6.69 7.19 Secchi depth (inches) >26.0 29.00 >26.0 29.00 Near bottom Temperature (°C) 14.8 17.6 14.8 17.6 Dissolved oxygen (%) 40.1 71.0 40.1 71.0 Dissolved oxygen (mg/L) 4.00 6.60 4.00 6.60 Specific conductivity (mS) 3.94 4.53 3.94 4.53 Conductivity (mS) 3.18 3.89 3.18 3.89 Salinity (psu) 2.10 2.43 2.10 2.43 pH 6.69 7.16 6.69 7.16 Table 1-102. Hydrographic parameters measured at Long Creek during 18 May 2021 benthic ponar grab and sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 24.5 23.7 24.5 23.7 Dissolved oxygen (%) 67.3 95.0 67.3 95.0 Dissolved oxygen (mg/L) 5.46 7.91 5.46 7.91 Specific conductivity (mS) 5.24 5.07 5.24 5.07 Conductivity (mS) 5.19 4.94 5.19 4.94 Salinity (psu) 2.82 2.72 2.82 2.72 pH 6.99 7.36 6.99 7.36 Secchi depth (inches) 29.00 30.00 29.00 30.00 Near bottom Temperature (°C) 24.3 22.9 24.3 22.9 Dissolved oxygen (%) 57.3 77.7 57.3 77.7 Dissolved oxygen (mg/L) 4.67 6.27 4.67 6.27 Specific conductivity (mS) 5.25 5.09 5.25 5.09 Conductivity (mS) 5.19 4.87 5.19 4.87 Salinity (psu) 2.82 2.74 2.82 2.74 pH 6.93 7.23 6.93 7.23 1-112 Table 1-103. Hydrographic parameters measured at Tooley Creek during 18 May 2021 benthic ponar grab and sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 26.4 26.0 26.4 26.0 Dissolved oxygen (%) 105.4 108.8 105.4 108.8 Dissolved oxygen (mg/L) 8.40 8.66 8.40 8.66 Specific conductivity (mS) 5.12 5.23 5.12 5.23 Conductivity (mS) 5.24 5.32 5.24 5.32 Salinity (psu) 2.75 2.80 2.75 2.80 pH 7.19 7.98 7.19 7.98 Secchi depth (inches) 30.00 30.00 30.00 30.00 Near bottom Temperature (°C) 24.6 22.9 24.6 22.9 Dissolved oxygen (%) 84.2 95.7 84.2 95.7 Dissolved oxygen (mg/L) 6.88 7.99 6.88 7.99 Specific conductivity (mS) 5.13 5.23 5.13 5.23 Conductivity (mS) 5.10 5.09 5.10 5.09 Salinity (psu) 2.75 2.83 2.75 2.83 pH 7.16 7.88 7.16 7.88 1-113 Table 1-104. Hydrographic parameters measured at Muddy Creek during 18 May 2021 benthic ponar grab and sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 24.0 23.0 24.0 23.0 Dissolved oxygen (%) 85.3 87.4 85.3 87.4 Dissolved oxygen (mg/L) 7.04 7.35 7.04 7.35 Specific conductivity (mS) 5.23 5.71 5.23 5.71 Conductivity (mS) 5.13 5.49 5.13 5.49 Salinity (psu) 2.82 3.09 2.82 3.09 pH 7.52 7.44 7.52 7.44 Secchi depth (inches) 21.00 22.50 21.00 22.50 Near bottom Temperature (°C) 23.0 22.5 23.0 22.5 Dissolved oxygen (%) 19.3 67.1 19.3 67.1 Dissolved oxygen (mg/L) 1.61 5.71 1.61 5.71 Specific conductivity (mS) 5.50 5.71 5.50 5.71 Conductivity (mS) 5.30 5.44 5.30 5.44 Salinity (psu) 2.98 3.09 2.98 3.09 pH 7.13 7.34 7.13 7.34 1-114 Table 1-105. Hydrographic parameters measured at Huddles Cut during 19 May 2021 benthic ponar grab and sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 24.5 26.9 24.5 26.9 Dissolved oxygen (%) 60.9 97.4 60.9 97.4 Dissolved oxygen (mg/L) 5.00 7.64 5.00 7.64 Specific conductivity (mS) 5.59 5.56 5.59 5.56 Conductivity (mS) 5.54 5.76 5.54 5.76 Salinity (psu) 3.02 2.99 3.02 2.99 pH 7.21 7.33 7.21 7.33 Secchi depth (inches) 12.00 >10 12.00 >10 Near_ Temperature (°C) Dissolved oxygen (%) Dissolved oxygen (mg/L) Specific conductivity (mS) Conductivity (mS) Salinity (psu) pH 23.9 61.4 5.09 6.03 5.91 3.27 7.11 26.9 97.4 7.64 5.56 5.76 2.99 7.33 23.9 61.4 5.09 6.03 5.91 3.27 7.11 26.9 97.4 7.64 5.56 5.76 2.99 7.33 1-115 Table 1-106. Hydrographic parameters measured at Porter Creek during 11 May 2021 benthic ponar grab sampling and benthic sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 22.0 21.7 22.0 21.7 Dissolved oxygen (%) 89.8 102.5 89.8 102.5 Dissolved oxygen (mg/L) 7.80 8.75 7.80 8.75 Specific conductivity (mS) 2.17 2.33 2.17 2.33 Conductivity (mS) 2.05 2.19 2.05 2.19 Salinity (psu) 1.11 1.20 1.11 1.20 pH 7.33 7.79 7.33 7.79 Secchi depth (inches) 20.00 29.50 20.00 29.50 Near bottom Temperature (°C) 22.0 21.3 22.0 21.3 Dissolved oxygen (%) 84.5 89.1 84.5 89.1 Dissolved oxygen (mg/L) 7.35 7.85 7.35 7.85 Specific conductivity (mS) 2.17 2.70 2.17 2.70 Conductivity (mS) 2.05 2.51 2.05 2.51 Salinity (psu) 1.11 1.40 1.11 1.40 pH 7.28 7.49 7.28 7.49 1-116 Table 1-107. Hydrographic parameters measured at DCUT11 during 11 May 2021 benthic ponar grab sampling and benthic sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 21.5 21.9 21.5 21.9 Dissolved oxygen (%) 74.1 89.6 74.1 89.6 Dissolved oxygen (mg/L) 6.51 7.77 6.51 7.77 Specific conductivity (mS) 2.01 1.95 2.01 1.95 Conductivity (mS) 1.87 1.83 1.87 1.83 Salinity (psu) 1.02 0.99 1.02 0.99 pH 6.66 7.43 6.66 7.43 Secchi depth (inches) >25.00 14.00 >25.00 14.00 Near bottom Temperature (°C) 21.3 21.9 21.3 21.9 Dissolved oxygen (%) 54.0 85.6 54.0 85.6 Dissolved oxygen (mg/L) 4.76 7.39 4.76 7.39 Specific conductivity (mS) 2.12 1.94 2.12 1.94 Conductivity (mS) 1.97 1.83 1.97 1.83 Salinity (psu) 1.09 0.99 1.09 0.99 pH 6.52 7.15 6.52 7.15 1-117 Table 1-108. Hydrographic parameters measured at DCUT19 during 11 May 2021 benthic ponar grab and sweep sampling Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 20.4 22.0 20.4 22.0 Dissolved oxygen (%) 44.5 85.6 44.5 85.6 Dissolved oxygen (mg/L) 3.95 7.34 3.95 7.34 Specific conductivity (mS) 2.59 2.30 2.59 2.30 Conductivity (mS) 2.37 2.16 2.37 2.16 Salinity (psu) 1.34 1.18 1.34 1.18 pH 6.26 7.33 6.26 7.33 Secchi depth (inches) >17.00 >21.00 >17.00 >21.00 Near bottom Temperature (°C) 20.5 22.0 20.5 22.0 Dissolved oxygen (%) 36.5 85.6 36.5 85.6 Dissolved oxygen (mg/L) 3.25 7.34 3.25 7.34 Specific conductivity (mS) 2.65 2.30 2.65 2.30 Conductivity (mS) 2.41 2.16 2.41 2.16 Salinity (psu) 1.37 1.18 1.37 1.18 pH 6.30 7.33 6.30 7.33 >21.00 >21.00 1-118 Table 1-109. Hydrographic parameters measured at Duck Creek during 12 May 2021 benthic ponar grab sampling and benthic sweep sampling. Parameter Ponar Sweep Upstream Downstream Upstream Downstream Near surface Temperature (°C) 20.1 20.3 20.1 20.3 Dissolved oxygen (%) 17.2 78.1 17.2 78.1 Dissolved oxygen (mg/L) 1.55 7.06 1.55 7.06 Specific conductivity (mS) 1.01 1.04 1.01 1.04 Conductivity (mS) 0.92 0.99 0.92 0.99 Salinity (psu) 0.50 0.54 0.50 0.54 pH 6.80 7.27 6.80 7.27 Secchi depth (inches) >24.00 30.00 >24.00 30.00 Near bottom Temperature (°C) Dissolved oxygen (%) Dissolved oxygen (mg/L) Specific conductivity (mS) Conductivity (mS) Salinity (psu) pH 20.1 14.3 1.29 1.01 0.92 0.50 6.64 20.3 76.5 6.84 1.08 0.98 0.54 7.22 20.1 14.3 1.29 1.01 0.92 0.50 6.64 20.3 76.5 6.84 1.08 0.98 0.54 7.22 1-119