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HomeMy WebLinkAboutNC0020800_Renewal Application_20170315NPDES DOCYNENT SCANNING COVER SHEET NPDES Permit: NC0020800 Andrews WWTP Document Type: Permit Issuance Wasteload Allocation Authorization to Construct (AtC) Permit Modification Complete File - Historical Renewal Application Speculative Limits Instream Assessment (67b) Environmental Assessment (EA) Permit History Document Date: March 15, 2017 This document is printed on reuse paper - ignore any content on the rezrerse side ..,CILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE FORM 2A NPDES FORM 2A APPLICATION OVERVIEW NPDES APPLICATION OVERVIEW Form 2A has been developed in a modular format and consists of a "Basic Application Information" packet and a "Supplemental Application Information" packet. The Basic Application Information packet is divided into two parts. All applicants must complete Parts A and C. Applicants with a design flow greater than or equal to 0.1 mgd must also complete Part B. Some applicants must also complete the Supplemental Application Information packet. The following items explain which parts of Form 2A you must complete. BASIC APPLICATION INFORMATION: A. Basic Application Information for all Applicants. All applicants must complete questions A.1 through A.8. A treatment works that discharges effluent to surface waters of the United States must also answer questions A.9 through A.12. Additional Application Information for Applicants with a Design Flow z 0.1 mgd. All treatment works that have design flows greater than or equal to 0.1 million gallons per day must complete questions B.1 through B.6. C Certification. All applicants must complete Part C (Certification). SUPPLEMENTAL APPLICATION INFORMATION: D Expanded Effluent Testing Data. A treatment works that discharges effluent to surface waters of the United States and meets one or more of the following criteria must complete Part D (Expanded Effluent Testing Data): 1. Has a design flow rate greater than or equal to 1mgd, 2. Is required to have a pretreatment program (or has one in place), or 3. Is otherwise required by the permitting authority to provide the information. Toxicity Testing Data. A treatment works that meets one or more of the following criteria must complete Part E (Toxicity Testing Data): 1. Has a design flow rate greater than or equal to 1 mgd, 2. Is required to have a pretreatment program (or has one in place), or 3. Is otherwise required by the permitting authority to submit results of toxicity testing. Industrial User Discharges and RCRA/CERCLA Wastes. A treatment works that accepts process wastewater from any significant industrial users (SIUs) or receives RCRA or CERCLA wastes must complete Part F (Industrial User Discharges and RCRA/CERCLA Wastes). SIUs are defined as: 1. All industrial users subject to Categorical Pretreatment Standards under 40 Code of Federal Regulations (CFR) 403.6 and 40 CFR Chapter I, Subchapter N (see instructions); and 2. Any other industrial user that: a. Discharges an average of 25,000 gallons per day or more of process wastewater to the treatment works (with certain exclusions); or b. Contributes a process wastestream that makes up 5 percent or more of the average dry weather hydraulic or organic capacity of the treatment plant; or c. Is designated as an SIU by the control authority. . Combined Sewer Systems. A treatment works that has a combined sewer system must complete Part G (Combined Sewer Systems). ALL APPLICANTS MUST COMPLETE PART C (CERTIFICATION) EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 1 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE BASIC APPLICATION INFORMATION PART A. BASIC APPLICATION INFORMATION FOR ALL APPLICANTS: All treatment works must complete questions A.1 through A.8 of this Basic Application Information Packet. A.1. Facility Information. Facility Name ANDREWS WASTEWATER TREATMENT PLANT Mailing Address PO BOX 1210 ANDREWS, NC 28901 Contact Person NANCY CURTIS TIM WOOD Title MAYOR WWTP ORC Telephone Number (828) 321-3113 (828) 321-3006 Facility Address 464 REAGAN AVE. (not P.O. Box) ANDREWS, NC 28901 A.2. Applicant Information. If the applicant is different from the above, provide the following: Applicant Name TOWN OF ANDREWS Mailing Address PO BOX 1210 ANDREWS, NC 28901 Contact Person NANCY CURTIS Title MAYOR Telephone Number (828) 321-3113 Is the applicant the owner or operator (or both) of the treatment works? ® owner ❑ operator Indicate whether correspondence regarding this permit should be directed to the facility or the applicant. ❑ facility ® applicant A.3. Existing Environmental Permits. Provide the permit number of any existing environmental permits that have been issued to the treatment works (include state -issued permits). NPDES NC0020800 PSD UIC Other RCRA Other A.4. Collection System Information. Provide information on municipalities and areas served by the facility. Provide the name and population of each entity and, if known, provide information on the type of collection system (combined vs. separate) and its ownership (municipal, private, etc.). Name Population Served Type of Collection System Ownership Town of Andrews 1500 Separate Municipal Total population served EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 2 of 22 51 Ir'ACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE A.5. Indian Country. a. Is the treatment works located in Indian Country? ❑ Yes ® No b. Does the treatment works discharge to a receiving water that is either in Indian Country or that is upstream from (and eventually flows through) Indian Country? ❑ Yes ®No A.6. Flow. Indicate the design flow rate of the treatment plant (i.e., the wastewater flow rate that the plant was built to handle). Also provide the average daily flow rate and maximum daily flow rate for each of the last three years. Each years data must be based on a 12-month time period with the 12th month of "this year" occurring no more than three months prior to this application submittal. a. Design flow rate 1.5 mgd Two Years Aqo b. Annual average daily flow rate 0.616 (2014) c. Maximum daily flow rate 1.850 (10/15/14) Last Year This Year 0.713 (2015) 0.570 (2016) 2.143 (12/31115) 1.961 (2/25/16) A.7. Collection System. Indicate the type(s) of collection system(s) used by the treatment plant. Check all that apply. Also estimate the percent contribution (by miles) of each. ® Separate sanitary sewer 100 0 Combined storm and sanitary sewer % A.8. Discharges and Other Disposal Methods. a. Does the treatment works discharge effluent to waters of the U.S.? ® Yes 0 No If yes, list how many of each of the following types of discharge points the treatment works uses: i. Discharges of treated effluent 1 ii. Discharges of untreated or partially treated effluent None iii. Combined sewer overflow points iv. Constructed emergency overflows (prior to the headworks) v. Other b. Does the treatment works discharge effluent to basins, ponds, or other surface impoundments that do not have outlets for discharge to waters of the U.S.? ❑ Yes If yes, provide the following for each surface impoundment: Location: N/A N/A N/A ® No Annual average daily volume discharge to surface impoundment(s) mgd Is discharge 0 continuous or 0 intermittent? c. Does the treatment works land -apply treated wastewater? 0 Yes ® No If yes, provide the following for each land application site: Location: Number of acres: Annual average daily volume applied to site: mgd Is land application 0 continuous or 0 intermittent? d. Does the treatment works discharge or transport treated or untreated wastewater to another treatment works? ❑Yes ®No EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 3 of 22 IiACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NCOO2O8OO PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE If yes, describe the mean(s) by which the wastewater from the treatment works is discharged or transported to the other treatment works (e.g., tank truck, pipe). If transport is by a party other than the applicant, provide: Transporter Name Mailing Address Contact Person Title Telephone Number ( ) For each treatment works that receives this discharge, provide the following: Name Mailing Address Contact Person Title Telephone Number ( ) If known, provide the NPDES permit number of the treatment works that receives this discharge Provide the average daily flow rate from the treatment works into the receiving facility. mgd e. Does the treatment works discharge or dispose of its wastewater in a manner not included in A.B. through A.8.d above (e.g., underground percolation, well injection): If yes, provide the following for each disposal method: Description of method (including location and size of site(s) if applicable): ❑ Yes ® No Annual daily volume disposed by this method: Is disposal through this method 0 continuous or 0 intermittent? EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 4 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE WASTEWATER DISCHARGES: If you answered "Yes" to question A.8.a, complete questions A.9 through A.12 once for each outfall (including bypass points) through which effluent is discharged. Do not include information on combined sewer overflows in this section. If you answered "No" to question A.8.a, go to Part B, "Additional Application Information for Applicants with a Design Flow Greater than or Equal to 0.1 mgd." A.9. Description of Outfall. a. Outfall number 001 b. Location VALLEY RIVER, ANDREWS. NC. 28901 (City or town, if applicable) (Zip Code) CHEROKEE (County) NC (State) 35°11'51' 83°50'46' (Latitude) (Longitude) c. Distance from shore (if applicable) N/A ft. d. Depth below surface (if applicable) N/A ft. e. Average daily flow rate 0.633 mgd f. Does this outfall have either an intermittent or a periodic discharge? ❑ Yes ® No (go to A.9.g.) If yes, provide the following information: Number f times per year discharge occurs: Average duration of each discharge: Average flow per discharge: mgd Months in which discharge occurs: g. Is outfall equipped with a diffuser? 0 Yes ® No A.10. Description of Receiving Waters. a. Name of receiving water VALLEY RIVER b. Name of watershed (if known) HIWASSEE RIVER WATERSHED United States Soil Conservation Service 14-digit watershed code (if known): c. Name of State Management/River Basin (if known): United States Geological Survey 8-digit hydrologic cataloging unit code (if known): d. Critical low flow of receiving stream (if applicable) acute cfs chronic cfs e. Total hardness of receiving stream at critical low flow (if applicable): _ mg/I of CaCO3 EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 5 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE A.11. Description of Treatment a. What level of treatment are provided? Check all that apply. ® Primary ® Secondary ❑ Advanced 0 Other. Describe: b. Indicate the following removal rates (as applicable): Design BOD5 removal or Design CBOD5 removal 85 % Design SS removal 85 % Design P removal % Design N removal Other % c. What type of disinfection is used for the effluent from this outfall? If disinfection varies by season, please describe: CHLORINATION If disinfection is by chlorination is dechlorination used for this outfall? ® Yes ❑ No Does the treatment plant have post aeration? ❑ Yes ® No A.12. Effluent Testing Information. All Applicants that discharge to waters of the US must provide effluent testing data for the following parameters. Provide the indicated effluent testing required by the permitting authority for each outfall through which effluent is discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QAIQC requirements of 40 CFR Part 136 and other appropriate QAIQC requirements for standard methods for analytes not addressed by 40 CFR Part 136. At a minimum, effluent testing data must be based on at least three samples and must be no more than four and one-half years apart. Outfall number: 001 PARAMETER MAXIMUM DAILY VALUE AVERAGE DAILY VALUE Value Units Value Units Number of Samples pH (Minimum) 5.8 s.u. ipH (Maximum) 7.4 s.u. 0.684 MGD 1460 Flow Rate 2.685 MGD Temperature (Winter) 16.1 °C 11.35 °C 92 Temperature (Summer) 25.1 °C 21.5 °C 92 • For pH please report a minimum and a maximum daily value POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD MLJMDL Conc. Units Conc. Units Number of Samples CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS BIOCHEMICAL OXYGEN DEMAND (Report one) BOD5 74.8 MG/L 23.3 MG/L 1460 SM5210B 2.0 CBOD5 FECAL COLIFORM >9000 #/100ML 62 #I100ML 1460 SM9222D 1/100m1 TOTAL SUSPENDED SOLIDS (TSS) 95.6 MG/L 19.7 MG/L 1460 SM2540D 5.0 END OF PART A. REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS OF FORM 2A YOU MUST COMPLETE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 6 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE BASIC APPLICATION INFORMATION PART B. ADDITIONAL APPLICATION INFORMATION FOR APPLICANTS WITH A DESIGN FLOW GREATER THAN OR EQUAL TO 0.1 MGD (100,000 gallons per day). All applicants with a design flow rate z 0.1 mgd must answer questions B.1 through B.6. All others go to Part C (Certification). B.1. Inflow and Infiltration. Estimate the average number of gallons per day 100,000 gpd that flow into the treatment works from inflow and/or infiltration. Collection Dept. Briefly explain any steps underway or planned to minimize inflow and infiltration. Ongoing identification and repair of problem areas by Sewer B.2. Topographic Map. Attach to this application a topographic map of the area extending at least one mile beyond facility property boundaries. This map must show the outline of the facility and the following information. (You may submit more than one map if one map does not show the entire area.) a. The area surrounding the treatment plant, including all unit processes. b. The major pipes or other structures through which wastewater enters the treatment works and the pipes or other structures through which treated wastewater is discharged from the treatment plant. Include outfalls from bypass piping, if applicable. c. Each well where wastewater from the treatment plant is injected underground. d. Wells, springs, other surface water bodies, and drinking water wells that are: 1) within'/4 mile of the property boundaries of the treatment works, and 2) listed in public record or otherwise known to the applicant. e. Any areas where the sewage sludge produced by the treatment works is stored, treated, or disposed. f. If the treatment works receives waste that is classified as hazardous under the Resource Conservation and Recovery Act (RCRA) by truck, rail, or special pipe, show on the map where the hazardous waste enters the treatment works and where it is treated, stored, and/or disposed. 8.3. Process Flow Diagram or Schematic. Provide a diagram showing the processes of the treatment plant, including all bypass piping and all backup power sources or redunancy in the system. Also provide a water balance showing all treatment units, including disinfection (e.g.. chlorination and dechlorination). The water balance must show daily average flow rates at influent and discharge points and approximate daily flow rates between treatment units. Include a brief narrative description of the diagram. B.4. Operation/Maintenance Performed by Contractor(s). Are any operational or maintenance aspects (related to wastewater treatment and effluent quality) of the treatment works the responsibility of a contractor? El Yes 0 No If yes, list the name, address, telephone number, and status of each contractor and describe the contractor's responsibilities (attach additional pages if necessary). Name: Earth Environmental Services Mailing Address: 75 Bison Ln. Murphy, NC. 28906 Telephone Number: (828) 837-9543 Responsibilities of Contractor: Laboratory Analysis/Sampling B.S. Scheduled improvements and Schedules of Implementation. Provide information on any uncompleted implementation schedule or uncompleted plans for improvements that will affect the wastewater treatment, effluent quality, or design capacity of the treatment works. If the treatment works has several different implementation schedules or is planning several improvements, submit separate responses to question 8.5 for each. (If none, go to question B.6.) a. List the outfall number (assigned in question A.9) for each outfall that is covered by this implementation schedule. 001 b. Indicate whether the planned improvements or implementation schedule are required by local, State, or Federal agencies. ❑ Yes ® No EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 7 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE c. If the answer to B.5.b is "Yes," briefly describe, including new maximum daily inflow rate (if applicable). N/A d. Provide dates imposed applicable. For improvements applicable. Indicate Implementation Stage - Begin Construction - End Construction - Begin Discharge - Attain Operational e. Have appropriate Describe briefly: by any compliance schedule planned independently dates as accurately as possible. Level permits/clearances concerning other or any actual dates of completion for the implementation steps listed of local, State, or Federal agencies, indicate planned or actual completion Schedule Actual Completion MM/DD/YYYY MM/DD/YYYY below, as dates, as 0 No 04/30/2017 (Tentative Date) (Tentative Date) / / / 12/31/2017 / / / / / / / / / Federal/State requirements been obtained? ® Yes B.6. EFFLUENT TESTING DATA (GREATER THAN 0.1 MGD Applicants that discharge to waters of the US must effluent testing required by the permitting authority on combine sewer overflows in this section. All information using 40 CFR Part 136 methods. In addition, this data QA/QC requirements for standard methods for analytes based on at least three pollutant scans and must be Outfall Number: 001 ONLY). provide effluent testing data for the following parameters. Provide for each outfall through which effluent is discharged. Do not include the indicated information conducted other appropriate data must be reported must be based on data collected through analysis must comply with QA/QC requirements of 40 CFR Part 136 and not addressed by 40 CFR Part 136. At a minimum effluent testing no more than four and on -half years old. POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD ML/MDL Conc. Units Conc, Units Number of Samples CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS AMMONIA (as N) CHLORINE (TOTAL RESIDUAL, TRC) DISSOLVED OXYGEN TOTAL KJELDAHL NITROGEN (TKN) NITRATE PLUS NITRITE NITROGEN OIL and GREASE PHOSPHORUS (Total) TOTAL DISSOLVED SOLIDS (TDS) OTHER END OF PART B. REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS OF FORM 2A YOU MUST COMPLETE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. I'2pc: 5 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WVVTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE ...(].1^- 1. *•� i. :7� 9-i -,�:J •y' ,v :..+ ?. �. h! L, Y •- w.' BASIC AP LICA.TION;I VFORMeATIO,�V— `- = t ' �'' Y .; kf . ,. 4. ,0. Y �:+'i`.►?ts.. i. r �•'1... L:,-.. .`.•'-^rc.[:S T-, _ .� "' A. ...., =;- :k s.x.. tt.' _ i ' • -- [ ... _ ..,,,,..C.4p =A sqb..] :•rF!'O , [ 'Gti 1r.-.Y✓Sz-1.'Ya �r µ1.- •-Y , . - _-• PART C:•. CERTIEiCATION� I:e �, q '� _,, *g { ,• '� ..s Sr... nC 2 �7 All applicants must complete the Certification Section. Refer to instructions to determine who is an officer for the purposes of this certification. All applicants must complete all applicable sections of Form 2A, as explained in the Application Overview. Indicate below which parts of Form 2A you have completed and are submitting. By signing this certification statement, applicants confirm that they have reviewed Form 2A and have completed all sections that apply to the facility for which this application is submitted. Indicate which parts of Form 2A you have completed and are submitting: ® Basic Application Information packet Supplemental Application Information packet: ® Part D (Expanded Effluent Testing Data) ® Part E (Toxicity Testing: Biomonitoring Data) ® Part F (Industrial User Discharges and RCRA/CERCLA Wastes) 0 Part G (Combined Sewer Systems) )5..4:_ 4.: + .N Lz�`:-ii•1c�. f '- -s •-•1-.` ti.•` . „,- � t5-..i 'c- lads-. .. '4,f� e.', M'c7`. r ..'•?•� - . _ r ALL APPLICANTS MUST`COMPLETE, THE FOLLOWINGCERTIF.ICATION ''. �. k I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system or those persons directly responsible for gathering the information, the information is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Name and official title Mayor Nancy Curtis -740Signature ��e Telephone number (828) 321-3113 - Date signed 3 / , 5 )17 Upon request of the permitting authority, you must submit any other information necessary to assure wastewater treatment practices at the treatment works or identify appropriate permitting requirements. SEND COMPLETED FORMS TO: NCDENR! DWR Attn: NPDES Unit 1617 Mail Service Center Raleigh, North Carolina 27699-1617 EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 9 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE SUPPLEMENTAL APPLICATION INFORMATION PART D. EXPANDED EFFLUENT TESTING DATA Refer to the directions on the cover page to determine whether this section applies to the treatment works. Effluent Testing: 1.0 mgd and Pretreatment Works. If the treatment works has a design flow greater than or equal to 1.0 mgd or it has (or is required to have) a pretreatment program, or is otherwise required by the permitting authority to provide the data, then provide effluent testing data for the following pollutants. Provide the indicated effluent testing information and any other information required by the permitting authority for each outfall through which effluent is discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected through analyses conducted using 40 CFR Part 136 methods. In addition, these data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. Indicate in the blank rows provided below any data you may have on pollutants not specifically listed in this form. At a minimum, effluent testing data must be based on at least three pollutant scans and must be no more than four and one-half years old. Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.) POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD ML/MDL Conc. Units Mass Units Conc. Units Mass Units Number of Samples METALS (TOTAL RECOVERABLE), CYANIDE, PHENOLS, AND HARDNESS. ANTIMONY ARSENIC 0.010 Mg/I 0.010 Mg/I 3 BERYLLIUM CADMIUM 0.001 Mg/I 0.001 Mg/I 3 CHROMIUM 0.005 Mg/I 0.005 Mg/I 3 COPPER 0.067 Mg/I 0.033 Mg/I 3 LEAD 0.005 Mg/I 0.005 Mg/I 3 MERCURY 0.0002 Mg/I 0.0002 Mg/I 3 NICKEL 0.010 Mg/I 0.010 Mg/I 3 SELENIUM 0.010 Mg/I 0.010 Mg/I 3 SILVER 0.005 Mg/I 0.005 Mg/I 3 THALLIUM ZINC 0.080 Mg/I 0.044 Mg/I 3 CYANIDE 0.042 Mg/I 0.010 Mg/I 3 TOTAL PHENOLIC COMPOUNDS HARDNESS (as CaCO3) Use this space (or a separate sheet) to provide information on other metals requested by the permit writer EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 10 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.) POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD MUMDL Conc. Units Mass Units Conc. Units Mass Units Number of Samples VOLATILE ORGANIC COMPOUNDS ACROLEIN ACRYLONITRILE BENZENE BROMOFORM CARBON TETRACHLORIDE CHLOROBENZENE CHLORODIBROMO- METHANE CHLOROETHANE 2-CHLOROETHYLVINYL ETHER CHLOROFORM DICHLOROBROMO- METHANE 1,1-DICHLOROETHANE 1,2-DICHLOROETHANE TRANS-I,2-DICHLORO- ETHYLENE 1,1-DICHLORO- ETHYLENE 1,2-DICHLOROPROPANE 1,3-DICHLORO- PROPYLENE ETHYLBENZENE METHYL BROMIDE METHYL CHLORIDE METHYLENE CHLORIDE 1,1, 2, 2-TETRA- CHLOROETHANE TETRACHLORO- ETHYLENE TOLUENE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 11 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.) POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD MLIMDL Conc. Units Mass Units Conc. Units Mass Units Number of Samples 1,1,1- TRICHLOROETHANE 1,1,2- TRICHLOROETHANE TRICHLOROETHYLENE VINYL CHLORIDE Use this space (or a separate sheet) to provide information on other volatile organic compounds requested by the permit writer ACID -EXTRACTABLE COMPOUNDS P-CHLORO-M-CRESOL 2-CHLOROPHENOL 2,4-DICHLOROPHENOL 2,4-DIMETHYLPHENOL 4,6-DINITRO-O-CRESOL 2,4-DINITROPHENOL 2-NITROPHENOL 4-NITROPHENOL PENTACHLOROPHENOL PHENOL 2,4,6- TRICHLOROPHENOL Use this space (or a separate sheet) to provide information on other acid -extractable compounds requested by the permit writer BASE -NEUTRAL COMPOUNDS ACENAPHTHENE ACENAPHTHYLENE ANTHRACENE BENZIDINE BENZO(A)ANTHRACENE BENZO(A)PYRENE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 12 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Outfall number: 001 (Complete once for each outfall discharging effluent to waters of the United States.) POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD MLIMDL Conc. Units Mass Units Conc. Units Mass Units Number of Samples 3,4 BENZO- FLUORANTHENE BENZO(GHI)PERYLENE BENZO(K) FLUORANTHENE BIS (2-CHLOROETHOXY) METHANE BIS (2-CHLOROETHYL)- ETHER BIS (2-CHLOROISO- PROPYL) ETHER BIS (2-ETHYLHEXYL) PHTHALATE 4-BROMOPHENYL PHENYL ETHER BUTYL BENZYL PHTHALATE 2-CHLORO- NAPHTHALENE 4-CHLORPHENYL PHENYL ETHER CHRYSENE DI-N-BUTYL PHTHALATE DI-N-OCTYL PHTHALATE DIBENZO(A,H) ANTHRACENE 1,2-DICHLOROBENZENE 1,3-DICHLOROBENZENE 1,4-DICHLOROBENZENE 3,3-DICHLORO- BENZIDINE DIETHYL PHTHALATE DIMETHYL PHTHALATE 2,4-DINITROTOLUENE 2,6-DINITROTOLUENE 1,2-DIPHENYL- HYDRAZINE EPA Form 3510-2A (Rev. 1-93). Replaces EPA forms 7550-6 & 7550-22. Page 13 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Outfall number: 001 (Complete once for each outfa I discharging effluent to waters of the United States.) POLLUTANT MAXIMUM DAILY DISCHARGE AVERAGE DAILY DISCHARGE ANALYTICAL METHOD ML/MDL Conc. Units Mass Units Conc. Units Mass Units Number of Samples FLUORANTHENE FLUORENE HEXACHLOROBENZENE HEXACHLORO- BUTADIENE HEXACHLOROCYCLO- PENTADIENE HEXACHLOROETHANE INDENO(1,2,3-CD) PYRENE ISOPHORONE NAPHTHALENE NITROBENZENE N-NITROSODI-N- PROPYLAMINE N-NITROSODI- METHYLAMINE N-NITROSODI- PHENYLAMINE PHENANTHRENE PYRENE 1,2,4- TR ICHLOROBENZENE Use this space (or a separate sheet) to provide information on other base -neutral compounds requested by the permit writer Use this space (or a separate sheet) to provide information on other pollutants (e.g , pesticides) requested by the permit writer END OF PART D. REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS OF FORM 2A YOU MUST COMPLETE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550.6 & 7550-22. Page 14 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE SUPPLEMENTAL APPLICATION INFORMATION PART E. TOXICITY TESTING DATA POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the facility's discharge points: 1) POTWs with a design flow rate greater than or equal to 1.0 mgd; 2) POTWs with a pretreatment program (or those that are required to have one under 40 CFR Part 403); or 3) POTWs required by the permitting authority to submit data for these parameters. • At a minimum, these results must include quarterly testing for a 12-month period within the past 1 year using multiple species (minimum of two species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC requirements for standard methods for analytes not addressed by 40 CFR Part 136. • In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a toxicity reduction evaluation, if one was conducted. • If you have already submitted any of the information requested in Part E, you need not submit it again. Rather, provide the information requested in question E.4 for previously submitted information. If EPA methods were not used, report the reasons for using alternate methods. If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E. If no biomonitoring data is required, do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to complete. E.1. Required Tests. Indicate the number of whole effluent 0 chronic ❑ acute E.2. Individual Test Data. Complete the column per test (where each species * POLLUTANT ANALYSIS SCAN RESULTS ATTACHED toxicity tests conducted in the past four and one-half years. following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one constitutes a test). Copy this page Test number: if more than three tests are being reported. Test number: Test number: a. Test information. Test Species & test method number Age at initiation of test Outfall number Dates sample collected Date test started Duration b. Give toxicity test methods followed. Manual title Edition number and year of publication Page number(s) c. Give the sample collection method(s) used. For multiple grab samples, indicate the number of grab samples used. 24-Hour composite Grab d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each. Before disinfection After disinfection After dechlorination EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 15 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Test number: Test number: Test number: e. Describe the point in the treatment process at which the sample was collected. Sample was collected: f. For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both Chronic toxicity Acute toxicity g. Provide the type of test performed. Static Static -renewal Flow -through h. Source of dilution water. If laboratory water, specify type; if receiving water, specify source. Laboratory water Receiving water i. Type of dilution water. If salt water, specify "natural" or type of artificial sea salts or brine used. Fresh water Salt water j. Give the percentage effluent used for all concentrations in the test series. •-��4•yy�I.yu�y say % k. Parameters measured during the test. (State whether parameter meets test method specifications) pH Salinity Temperature Ammonia Dissolved oxygen I. Test Results. Acute: Percent survival in 100% effluent LC50 95% C.I. Control percent survival % % % Other (describe) EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 16 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE Chronic: NOEC IC25 % % % Control percent survival Other (describe) m. Quality Control/Quality Assurance. Is reference toxicant data available? Was reference toxicant test within acceptable bounds? What date was reference toxicant test run (MM/DD/YYYY)? / / ! / / / Other (describe) E.3. Toxicity Reduction Evaluation. ❑ Yes ❑ No Is the treatment works involved in a Toxicity Reduction Evaluation? If yes, describe: E.4. Summary of Submitted Biomonitoring Test Information. If you have cause of toxicity, within the past four and one-half years, provide the dates of the results. Date submitted: / / (MM/DD/YYYY) submitted biomonitoring test information, or information regarding the the information was submitted to the permitting authority and a summary Summary of results: (see instructions) END OF PART E. REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS OF FORM 2A YOU MUST COMPLETE. EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 17 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE SUPPLEMENTAL APPLICATION INFORMATION PART F.INDUSTRIAL USER DISCHARGES AND RCRA/CERCLA WASTES All treatment works receiving discharges from significant industrial users complete part F. GENERAL INFORMATION: or which receive RCRA,CERCLA, ot, an approved pretreatment program? Users (CIUs). Provide the number or other remedial wastes must of each of the following types of questions F.3 through F.8 and F.1. Pretreatment program. Does the treatment works have, or is subject ®Yes ID No F.2. Number of Significant Industrial Users (SlUs) and Categorical Industrial industrial users that discharge to the treatment works. a. Number of non -categorical Sills. 0 b. Number of ClUs. 1 SIGNIFICANT INDUSTRIAL USER INFORMATION: to the treatment works, copy Supply the following information for each SIU. If more than one SIU discharges provide the information requested for each SIU. F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages as necessary. Name: TEAM INDUSTRIES, INC. Mailing Address: 3750 AIRPORT RD. ANDREWS, NC. 28901 F.4. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge. METAL FINISHING, ELECTROPLATING, AND ANCILLARY PROCESSES F.5. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's discharge. Principal product(s): GEAR CASES Raw material(s): ALUMINUM AND OTHER MATERIALSIMETALS F.6. Flow Rate. a. Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into day (gpd) and whether the discharge is continuous or intermittent. *90,000 gpd ( continuous or X intermittent) *PERMITTED the collection system in gallons per FLOW into the collection system b. Non -process wastewater flow rate. Indicate the average daily volume of non -process wastewater flow discharged in gallons per day (gpd) and whether the discharge is continuous or intermittent. 25,000 gpd ( continuous or X intermittent) F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following: a. Local limits ® Yes ❑ No b. Categorical pretreatment standards ® Yes 0 No If subject to categorical pretreatment standards, which category and subcategory? 40 CFR 433 EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 18 of 22 FACILITY NAME AND PERMIT NUMBER: ANDREWS WWTP, NC0020800 PERMIT ACTION REQUESTED: RENEWAL RIVER BASIN: HIWASSEE F.8. Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e.g., upsets, interference) at the treatment works in the past three years? ❑ Yes ® No If yes, describe each episode. RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE: F.9. RCRA Waste. Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe? ❑ Yes EI No (go to F.12) F.10. Waste transport. Method by which RCRA waste is received (check all that apply): 0 Truck ❑ Rail ❑ Dedicated Pipe F.11. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units). EPA Hazardous Waste Number Amount Units CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER: F.12. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities? ❑ Yes (complete F.13 through F.15.) ® No F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to origniate in the next five years). F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received). Include data on volume and concentration, if known. (Attach additional sheets if necessary.) F.15. Waste Treatment. a. Is this waste treated (or will be treated) prior to entering the treatment works? ❑ Yes ❑ No If yes, describe the treatment (provide information about the removal efficiency): b. Is the discharge (or will the discharge be) continuous or intermittent? ❑ Continuous 0 Intermittent If intermittent, describe discharge schedule. END OF PART F. REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS OF FORM 2A YOU MUST COMPLETE EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 19 of 22 Name and Address of Facility: Town of Andrews WWTP P.O. Box 1210 Andrews.N.C. 28901 Long Term Monitoring Plan Samples -Effluent Earth EnviroNet8I Services Michael Ladd 75 Bison Lane Murphy, NC 28906 Robbinsville Lab (828) 479-6428 Madison County Lab (828) 649-9250 Residence (828) 837-9543 Date: 3/10/2015 Sample Collector. Date and Time Sample Received: Date and Time Sample Collected: Date and Time Sample Analyzed: MB 2/17-20/15 See 2/16-20/15 C.O.C. < MHT Test 2116-17/15 2/17-18/15 2/18-19/15 2/19-20/15 Report Limit Method Units BOD 35.1 19.2 13.6 17.3 2.0 SM5210B mg/L TOTAL SUSPENDED RESIDUE 25.0 22.8 16.3 18.3 1.0 SM2540D mg!L pH 6.7 6.6 6.5 6.3 0-14 SM4500H+ units AMMONIA NITROGEN 4.1 2.6 2.8 2.4 0.1 SM4500NH3F mg/L TEMPERATURE 9.4 8.2 6.4 6.3 0.1 SM2550B deg C CYANIDE TOTAL < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA-SMas00 CN E mg!L CADMIUM < 0.001 < 0.001 < 0.001 < 0.001 0.0010 SCA-EPA 200.7 mg/L CHROMIUM < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA-EPA 200.7 mg/L COPPER 0.008 0.006 0.006 0.006 0.001 SCA-EPA 200.7 mg/L LEAD < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA-EPA 200.7 mg!L NICKEL < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mg/L ZINC 0.042 0.032 0.032 - 0.033 0.010 SCA-EPA 200.7 mg/L SILVER < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA-EPA 200.7 mg/L ARSENIC < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mglL SELENIUM < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mglL MOLYBDENUM . < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mglL MERCURY < 0.00020 < 0.00020 < 0.00020 < 0.00020 0.00020 SCA-EPA 200.7 mg!L OIL & GREASE 5.1 < 5.0 < 5.0 < 5.0 5.0 SCA-EPA1664A mg/L N.C. Laboratory ID# 352 Analyzed By: Note: pH and Temperature performed as Field Analyses by EES Lab Supervisor. Michael J. Ladd; EES-Robbinsville Certified By: MB /naud te,,ta Earth EflviroRentaI Serices Name and Address of Facility: Town of Andrews WWTP P.O. Box 1210 Andrews.N.C. 28901 Long Term Monitoring Plan Samples -Effluent Michael Ladd 75 Bison Lane Murphy, NC 28906 Robbinsville Lab (828) 479-6428 Madison County Lab (828) 649-9250 Residence (828) 837-9543 Date: 6/16/2014 Sample Collector: Date and Time Sample Received: Date and Time Sample Collected: Date and Time Sample Analyzed: MB 5/20-23/14 See 5/19-23/14 C.O.C. < MHT Test 5/19-20/14 5/20-21/14 5/21-22/14 5/22-23/14 Report Limit Method Units BOD 23.1 16.6 16.9 15.4 2.0 SM5210B mg/L TOTAL SUSPENDED RESIDUE 14.3 8.1 8.5 8 1.0 SM2540D mg/L ..pH 6.6 6.1 6.1 6.0 0-14 SM4500H+ units 'AMMONIA NITROGEN 5.1 3.2 2.8 2.2 0.1 SM4500NH3F mg/L TEMPERATURE 17.8 16.5 17.2 18.2 0.1 SM2550B deg C CYANIDE TOTAL 0.006 0.006 0.015 0.023 0.005 SCA-SM4500 CN E mglL CADMIUM < 0.001 < 0.001 < 0.001 < 0.001 0.0010 SCA-EPA 200.7 mg/L CHROMIUM < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA-EPA 200.7 mglL COPPER 0.067 0.007 0.007 0.008 0.010 SCA-EPA 200.7 mg/L LEAD < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA-EPA 200.7 mglL NICKEL < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mg/L ZINC 0.037 0.037 0.036 0.043 0.030 SCA-EPA 200.7 mg/L SILVER < 0.005 < 0.005 < 0.005 < 0.005 0.005 SCA-EPA 200.7 mg/L ARSENIC < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mg/L SELENIUM < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mg/L MOLYBDENUM < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mg/L MERCURY < 0.00020 < 0.00020 < 0.00020 < 0.00020 0.00020 SCA-EPA 200.7 mg/L OIL & GREASE < 5.0 < 5.0 < 5.0 < 5.0 5.0 SCA-EPA1664A mg/L N.C. Laboratory ID# 352 Analyzed By: Note: pH and Temperature performed as Field Analyses by EES Lab Supervisor. Michael J. Ladd; EES-Robbinsville Certified By: MB Earth Environifiental Services Name and Address of Facility: Town of Andrews WWTP P.O. Box 1210 Andrews. N.C. 28901 Long Term Monitoring Plan Samples -Effluent Michael Ladd 75 Bison Lane Murphy, NC 28906 Robbinsville Lab (828) 479-6428 Madison County Lab (828) 649-9250 Residence (828) 837-9543 Date: 3/4/2013 Sample Collector Date and Time Sample Received: Date and Time Sample Collected: Date and Time Sample Analyzed: MB 2/5-8/13 See 2/4-8/13 C.O.C. < MHT Test 2/4-5/13 2/5-6/13 2/6-7/13 2/7-8/13 Report Limit Method Units BOD 21.8 35.6 25.1 11.5 2.0 SM5210B mg/L TOTAL SUSPENDED RESIDUE 7.0 21.0 14.5 < 5.0 1.0 SM2540D mglL pH 6.5 6.7 6.4 6.2 0-14 SM4500H+ units AMMONIA NITROGEN 1.4 2.1 1.6 0.9 0.1 SM4500NH3F mg/L TEMPERATURE 11.5 11.0 10.7 11.7 0.1 SM2550B deg C CYANIDE TOTAL < 0.0050 < 0.0050 < 0.0050 < 0.0050 0.0050 SCA-SM4500 CN E mglL CADMIUM < 0.0010 < 0.0010 < 0.0010 < 0.0010 0.0010 SCA-EPA 200.7 mg/L CHROMIUM < 0.0050 0.012 0.0055 < 0.0050 0.0050 SCA-EPA 200.7 mg/L COPPER < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mg/L LEAD < 0.0050 < 0.0050 < 0.0050 < 0.0050 0.0050 SCA-EPA 200.7 mg/L NICKEL < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mg/L ZINC < 0.030 0.042 < 0.030* < 0.030 0.030 SCA-EPA 200.7 mg/L SILVER < 0.0050 < 0.0050 < 0.0050 < 0.0050 0.0050 SCA-EPA 200.7 mg/L ARSENIC < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mglL SELENIUM < 0.010 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mg/L MOLYBDENUM < 0.010 < 0.010 < 0.010 < 0.010 0.010 SCA-EPA 200.7 mg/L MERCURY < 0.00020 < 0.00020 < 0.00020 < 0.00020 0.00020 SCA-EPA 200.7 mg/L ,OIL & GREASE < 5.0 < 5.0 < 5.0 < 5.0 5.0 SCA-EPA1664A ' mg/L N.C. Laboratory lD# 352 Analyzed By: Note: pH and Temperature performed as Field Analyses by EES Lab Supervisor. Michael J. Ladd; EES-Robbinsville Certified By: MB PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. March 25, 2016 Mr. Michael Ladd Earth Environmental Services 75 Bison Lane Murphy, NC 28906 RE: ETS PROJECT NUMBER: 11291 Dear Mr. Ladd: Enclosed are toxicity test results for samples from the Andrews WWTP received by Environmental Testing Solutions, Inc. March 08 through March 10, 2016. Parameter Test Procedure EPA Method Final Code Number Result TGP3B North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure (Ceriodaphnia Pass/Fail Toxicity Test) EPA-821-R-02-013 PASS If this test was performed as an NPDES requirement or by Administrative Letter, please enter a P on the Effluent Discharge Monitoring Form (MR-1) for the collection date March 08, 2016 using the parameter code TGP3B. Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT-3) by April 30, 2016. If you have any questions concerning these results, please feel free to contact me. Sincerely, I1I11 Limner Laboratory Director This report should not be reproduced. except in its entirety, without the written consent of Environmental Testing Solutions. Inc. The results in this report relate only to the samples submitted for analysis. North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600 South Carolina Certificate Number: Clean Water Act: 99053-001 .:4+ Environmental Testing Solutions, Inc. Effluent Aquatic Toxicity Report Form - Phase II Chronic Ceriodaphnia dubia PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Date: March 25.2016 Facility: Earth Environmental Services Andrews W VTP NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee Laboratory Performing Test: Environmental Testing Solutions, Inc. Comments: Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor: Project: 11291 Samples: 160308.15,160310.13 Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center Raleigh, NC 27699-1621 Sample Information Collection start date: Grab: Composite duration: Alkalinity (mg/L CaCO,): Hardness (mg/L CaCO3): Conductivity (µmhoslcm): Total residual chlorine (rng/L): Sample Temp. at Receipt CC): Control Organisms Sample t Sample 2 Control 03-07-16 03-09-16 24.25-h 25-h 30,31,32 45,42.37 251 243 149. 152. 156 < 0.10 <0.10 1.1 0.9 Organism Number 2 3 4 5 6 7 8 9 Test Information Treatment: Initial pH (SO): Final pH (SU): Initial DO (tng/L): Final DO (mg/L): Initial Temp. (°C): Final Temp. (°C): Start date: 03-09-16 End date: 03-16-16 Start time: 1348 End time: 0625 Sian Rcacnal 1 Rcncnal 2 Stan Rcncnal t Katmai 2 13% 13% 13% Control Control Control 7.38 7.17 7.08 7.51 7.31 7.08 7.16 7.13 7.10 7.16 7.14 7.05 7.6 7.6 8.0 7.8 7.7 7.8 7.8 7.9 7.9 7.8 7.8 7.8 25.0 25.3 24.8 24.8 24.8 24.9 25.1 25.1 25.1 25.1 24.9 25.0 Number of Young Produced 29 30 29 31 27 28 31 29 27 29 26 30 28.8 Adult Survival: (L)ive, (D)ead L L L L L LLL LLLL Effluent Percentage Treatment 2 Organisms 2 Number of Young Produced 32 35 36 35 34 34 35 33 33 34 36 34.1 Adult Survival: (L)ive, (D)ead LLLL LLLL _32 LLLL -18.2 Effluent Percentage Treatment 3 Organisms % Reduction 2 3 4 5 6 7 8 9 10 lI 12 Mean Number of Young Produced Adult Survival: (L)ive, (D)ead Effluent Percentage Treatment 4 Organisms Number of Young Produced Adult Survival: (L)ive, (D)ead t. Reduction I 2 3 4 5 6 7 8 9 10 11 12 mean Effluent Percentage Treatment 5 Organisms Number of Young Produced % Reduction 1 2 3 4 5 6 7 8 9 10 Il 12 clean Adult Survival: (L)ivc, (D)ead Effluent Percentage Treatment 6 Organisms Number of Young Produced Adult Survival: (L)ive, (D)ead '.: Reduction 2 3 4 5 6 7 8 9 10 Il 12 Mean Overall Analysis: Result: PASS LOEC: >13% NOEC: 13% ChV: >13% Reduction Chronic Test Results Final Control Mortality (%): 0.0 %Control with 3rd Broods: 100 Control Reproduction CV: 5.5 48 Hour Mortality Control: 0 of 12 1WC: 0 of 12 Significant?: No Final Mortality Significant at: No concentration Reproduction Analyses Reproduction LOEC: >13% Reproduction NOEC: 13% Overall Method: Homoscedastic t ,Normal Distribution: Yes Method: Shapiro-Wilk's Statistic: 0.950 Critical Value: 0.884 Equal Variances: Yes Method: F-Test Statistic: 1.323 Critical Value: 5.320 Non -Parametric Analysis (if applicable) Method: . Effluent % Rank Stun Critical Sum 13% MVO form AT-3 (8/91) Rev. 11/95 It I I I i i i i 1 1 1 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia (EPA-821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Control Bench Sheet Control #: ( Date: 03-09-16 Test Grouping Information: Facility Project # 6 5 'Tlu-1 Un t j\..) -P )1?A0 4 gelt*Linuil�i�P 1I7g2 112661 t 1 A I 3 1.mi0t.to kuu) fP 2 A net N.04 UJu.) -P 1- Control Daily Renewal Information: Test Organism Information: Page 1 of 1 Organism Source: In-house Culture Age: < 24-hours old 1 Source (culture board): 03-0%-tb PS I ,O, it € Replicate # 1 4 5 6 7 8 9 10 11 12 Culture board cup # Z Z'i :II:01 Bb 11 li 440 t '{ A to tl Date and time organisms were born between: 0b.Ch.1b 0Oa- i Vb0 .— Average transfer volume: 0.0121 mL Transfer bowl information: pH (S.U.): -VW Temperature (°C): -,A 1 Day Date Test Initiation, renewal, feeding or termination - SSW Batch Selenastrum Batch YWT Batch Location Incubator/Shelf Randomizing Template Time Analyst 0 03-09-16 initial` d'g y� a \ O3^i3•)6A O1:-L%-i o (rt25.iio Z-GZ eel) 1 03-10-16 Feeding . 2 ��16 03Qdi2 Renews /Fe d" g n 6 4.. 6 as.6.166 3 03-12-16 Feeding bk.' 4 03-13-16 Feeding (i30a' - N 5 03-14-16 Renewal.5O 2/FeedingOk u3 oar it. 6 03-15-16 Feed_i��.+g�O _ , 7 03-16-16 Termination °G�LS Chemical Analyses: Initiation Renewal One Renewal Two Initial Final Initial Final Initial Final Concentration Analyst )IV WO N6 NG Control, SSW •Analyst identified for each pH (S.U.) 1 1;51 1.1 to 1.' i 7./ / 7. Og 'Zar Dissolved oxygen (mg/L) '.,8 1, ?) -�.1 7,9 -7, g 7 d Conductivity (µmhos/cm) (� Q "; . 1 5'2. ! 51 `Alkalinity (mg/1 CaCO3) 30 3( 32. *Hardness (mg/LCaCO3) L Lib LiZ - 31• 'Temperature (°C) da erformed i.t A........1.....1 • -14, 8 $ 1 IA. q 1.4•6k 2 -ek 1 IS . 0 y, p p . oxygen and cunuuctrvny meal irements only temperatures performed at the tune of test initiation. renewal or termination by the analyst identified in the Daily Renewal Information table Alkalinity and hardness performed by the analysts indentitied on the test bench sheets and transcribed to this bench sheet Survival and Reproduction Data: Day Observations Replicate number 1 2 3 4 5 6 7 8 9 10 11 12 2 Renewal One Adult mortality (L = live, D = dead) � L-- L 1✓ 5 Renewal Two Number of broods present Number of young produced 1 S Li 1 1V Li 1.% L is L ) Li., 1'1 L lam{ 11 11-. 15 L 12 Li it., Adult mortality = Live, D = dead) L � \- x- L-(L 7 Final Number of broods present Number of young produced 1 L 16 L : `4 L Ito LL no � 13 t`1 L i 11 a a L S Lii IA 1q l Total young produced Z V Zk Z.� Z� 3t 1R Z1 Q Z ` lia 10 Final adult mortality (L=Live,0=dead) � � 1,— t L- L_ � l_ `— X for 3rd Broods X ) X x x ontrol Acceptance Criteria: of Male Adults (< 20%) Adults having 3rd Broods (? 80%) ton Mean Offspring/Female (>_ 15 offspring/surviving female) 0.31811. fJ� 2ae % Mortality (< 20%) U % CV (< 40%) 567 SOP AT12 • Exhibit AT12.3 revision tt.nt.tat Page 1 of 1 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia (EPA-821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Test Bench Sheet Paired with Control # I Date: 0343.W,, Client Earth Environmental Services Facility Andrews WWTP Project # IZ..C% Test Concentration (Chronic Limit) 13.0% Samples were not aerated or treated unless otherwise noted on this form. Control, dilution water and test renewal information are included on the Control Bench Sheet indicated above. NPDES # NC0020800 Outfall 001 County Cherokee Dilution preparation: mL Sample mL Dilution water, Total volume mL 39 261 300 Chemical Analyses: Initiation Renewal One Renewal Two Initial Final Initial Final Initial •Final Concentration Analyst 50 citt,6 i ti6 Test Concentration pH (S.U.) 7. 3Ii5 1' l to - M l i 7, /3 .O.6 7.0a Dissolved oxygen (mg/L) 7-to�- 9. 1- (p 7, -L i,49 7.4 Conductivity (µmhos/crn) 17 0 f , :.: I ae ' ` ' %, ` ti ; *Temperature (°c) 1S-0 'IS •‘ A-5•5 IS.\ 'L'1• f "LS. • 100%° pH (S.U.) 1. 7-to1.O1 . - ' V • :` • t `, Dissolved oxygen (mg/L) 'i.1 .. ( ; .. _. .. _, .. Conductivity (µmhos/cm) 251 21+3 i .. '',.--- s *Total residual chlorine (mg/L) 40.10 , - LO• 10 . _ ' ` :- ' Sample number Sample 1 tte o3ot, ,s Sample 2 l to a 1 a, t3• Analyst tdenufied for each day, performed pH, dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual Chlorine Bench Sheet and transcribed to this bench sheet. Survival and Reproduction Data (performed at test concentration) : Da y Observations Replicate number 1 2 3 4 5 6 7 8 9 10 11 12 2 Renewal One Adult mortality (L = Live, D =dead) � �-- L L. L. L. `- 5 Renewal Two Number of broods present Number of young produced L t S 'Lt.- t Occ� La- ' L tl cL La L Iek tl• . t t L t L IS L IA IA Adult mortality (L = Live, D = dead) L '- V- L L V_ L 7 Final Number of broods present Number of young produced a t-i Li. 11 L i L t L I L IL �ilkl L► S L 1 L (1 L S L Lp 1 Total young produced -b L 3S 36 3S sA 3%-. 3S b-S 33 31_ 's`t ND Final adult mortality (L = Live, D = dead) ,L.- L. L L. L.- - L V- L Sample 2 was used for Renewals one (day 2) and Two (day 5). Samples were diluted to the test concentration prior to use with soft synthetic water and warmed to 25.0 i 1.0°C in a warm water bath. Comments: Test Results and Statistical Analyses: Test results % Mortality , Mean offspring per female 341 % Reduction from control ,18,21 ' Statistics t-Stat or RankSum r gvsli 1-Tailed Critical 2 , 8 PASS or FAIL 10k55 SOP AT12 - Exhibit AT12.2, revision 11-01-14 Ceriodaphnia (labia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 - North Carolina Modification Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc. Client: Test dates: Project number: Reveiwed by: Andrews WW'1'P March 09 - 16, 2016 11291 Concentration (%) Day Number of young produced by replicate number Survival ("A.) Average reproduction (offspring/female) Coefficient of variation (%) Percent reduction from control CA) 1 2 3 4 5 6 7 8 9 10 11 12 Control 5 13 16 13 15 14 14 14 17 12 15 12 16 100 28.8 5.5 Not applicable 7 16 14 16 16 13 14 17 12 15 14 14 14 Total 29 30 29 31 27 28 31 29 27 29 26 30 13% 5 15 18 17 17 18 19 16 18 15 15 19 17 100 34.1 4.0 -18.2 7 17 17 19 18 16 15 19 15 18 17 15 19 Total 32 35 36 35 34 34 35 33 33 32 34 36 Dunnett's NISI) value: P%ISD: 1.522 5.3 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10`h percentile) = 13%. Upper PMSD bound determined by USEPA (90`h percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile. respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a: USEPA, 2001b). USEPA. 2001a. 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency. Cincinnati. OH. Andrews 03-09-14 Environmental Testing Solutions, Inc. Start Date: 3/9/2016 End Date: 3/16/2016 Sample Date: Comments: Ceriodaphnia Survival and Reproduction Test -Reproduction Test ID: CdPFCRNC Sample JD: Andrews VVWTP Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Conc% `:.-1..-..2 3 4 5 6 •: -9 10, D-Control 29.000 30.000 29.000 31.000 27.000 28.000 31.000 29.000 27.000 29.000 D-Control 26.000 30.000 13 32.000 35.000 36.000 35.000 34.000 34.000 35.000 33.000 33.000 32.000 13 34.000 36.000 Transform: Untransformed . 1-Tailed Conc% Mean N-Mean Mean Min Max.::; ,CV%.., ::N.. t-Stat .Critical;'. MSD D-Control 28.833 1.0000 28.833 26.000 31.000 5.500 12 13 34.083 1.1821 34.083 32.000 36.000 4.046 12 -8.654 2.508 1.522 Auxiliary Tests - Statistic Critical Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94975 0.884 F-Test indicates equal variances (p = 0.65) 1.32271 5.31963 Hypothesis Test (1-tail, 0.01) Homoscedastic t Test indicates no significant differences Treatments vs D-Control Skew Kurt -0.2526 -0.8427 MSDu _ MSDp MSB MSE F-Prob df 1.52174 0.05278 165.375 2.20833 1.6E-08 1, 22 Andrews 03-09-16 f44, Environmental Testing Solutions, Inc. Whole Effluent Toxicity Chain -of -Custody Form 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Facility: Earth Environmental Services, Inc. NPDES #: NC0020800 Pipe II: 001 County: Cherokee Andrews WWTP Species: Ceriodaphnia (labia Test type: Pass/Fail Chronic Purchase order: Effluent dilution: 13% Parameter code: TGP3B Sample information: (to be completed by sample collector) Composite sample: Start date: End date: Number of samples per hour: Chilled during collection? If chilled, specify temperature: Sample location: f &.&I A 4- 64 Time: e 4 3 .A Volume collected for testing: / %i tz-ef' Time: , i :tom Number of containers filled for testing: le s i 5i.lep,L Method of transport to laboratory: Comments: 7 Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: 1)11A1A+1 Print Relinquished by: Print Relinquished by: Signature Date and time Signature Date and lime Prins Signature Date and time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS y: /Yh L Print Signature Date and time Custody seals intact?: Yes No Samples received in good condition?: Tracking number: Not used Yes No Received by: b PL(i(v.0 Print Received by: Signature Date and time Print Received at ETS by: Signature Date and time Print Signature Sample temperature upon receipt at ETS (°C): Total residual chlorine upon receipt at ETS: (DPD PrescnccAbsense Indicator. MI)L - U.10 tng'L) Project number: 11211 Sample number: I1O03C9 LtS Q3, ts8.l)o lt,12%1 Date and time 1,1'4-- Present Absent Comments: +fir 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 , Environmental Testing Solutions, Inc. Whole Effluent Toxicity Chain -of -Custody Form Facility: Earth Environmental Services, Inc. Andrews WWTP Species: Ceriodaplinia dubia Test type: Pass/Fail Chronic NPDES #: NC0020800 Pipe #: 001 County: Cherokee Purchase order: Effluent dilution: 13% Parameter code: TGP3B Sample information: (to be completed by sample collector) Composite sample: Start date: End date: Number of samples per hour: Chilled during collection? If chilled, specify temperature: Time: Time: P teiv 0 Sample location: Rv 0 OEMs A S Volume collected for testing: g10 eat A-01,6 1 it led Number of containers filled for testing: cat. Method of transport to laboratory: Comments: Triple rinse sample container with sample before filling. E`er Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sam le collected by: 3/(6lr(,), Signature Date ar d time Print Relinquished by: L L Print Relinquished by: Signature Date and time Print Signature Date and time Received by: Print Received by: Signature Date and time Print Signature Date and time Sample receipt information: (to be completed by ETS personnel) Relinquished t ETS b o At CA' L te- dv (32s Print Custody seals intact?: Signature Ycs No Samples received in good condition?: Tracking number: Not used Yes Date and time No Received at ETS by: figa_ Ulm Print Signature Sample temperature upon receipt at ETS (°C): Total residual chlorine upon receipt at ETS: (DPD Prescttec:Absense Indicator, MDI. - 0.It) mg'l.) Project number: `t 1 Sample number: 14)0310,13 03•io►Fi (32C Date and time O.q•c, Present Absent Comments: 7-day IC25 (g/L NaCI) Environmental Testing Solutions, Inc. 1.14 1.12 1.10 1.08 1.06 1.04 1.02 2.5 2.0 1.5 1.0 0.5 1.4 1.3 1.2 1.0 0.9 0.8 a A a a 5 5 5 5 S b b b b 0_"\ 0..\ 0�.\ Oti"\ \. A 0�,\ t_A 0.. t 09"\ \A,s o�As \, 0, 0_As 0_As \ti,\ 09� 00,\ 0a� \0" \0" \\" \ti" " Oti" 0�" 0,, 05" 0.. " " Oq" \0 \\" \ti" o Oti" tvi 0.s" Ceriodaphnia dubia Chronic Reference Toxicant Control Chart I I I 1 I 1 I I I I I I I I I I I I USEPA Control Limits (± 2 Standard Deviations) 1 I I 1 1 1 1 1 1 1 1 1 1 1 1 1 I I 1 USEPA Warning and Control Limits (75th and 90th Percentile CVs) • •- +- •---- . --0---•--•—•—•---•- a •— -• • —• • -•• 1 1 1 1 1 1 1 I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Laboratory Warning and Control Limits (101h and 251t' Percentile CVs) 1 1 1 1 1 1 1 1 ► 1 1 1 1 1 1 1 1 1 1 Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC25) Warning Limits (mean IC25 ± SA 10 or SA 75) Control Limits (mean IC25 ± Sn 25, SA 90, or 2 Standard Deviations) aJnie.ea.e a.mwea by mmmn Q) 'dy; _:t Environmental Testing Solutions, Inc. June 27, 2016 Mr. Michael Ladd Earth Environmental Services 75 Bison Lane Murphy, NC 28906 RE: ETS PROJECT NUMBER: 11545 Dear Mr. Ladd: PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Enclosed are toxicity test results for samples from the Andrews WWTP received by Environmental Testing Solutions, Inc. June 14 through June 16, 2016. Parameter Test Procedure EPA Method Final Code Number Result TGP3B North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure (Ceriodaphnia Pass/Fail Toxicity Test) EPA-821-R-02-013 PASS If this test was performed as an NPDES requirement or by Administrative Letter, please enter a P on the Effluent Discharge Monitoring Form (MR-1) for the collection date June 14, 2016 using the parameter code TGP3B. Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT-3) by July 31, 2016. If you have any questions concerning these results, please feel free to contact me. Sincerely, Laboratory Director This report should not be reproduced, except in its entirety, without the written consent of Environmental Testing Solutions. Inc. The results in this report relate only to the samples submitted for analysis. North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600 South Carolina Certificate Number: Clean Water Act: 99053-001 Environmental Testing Solutions, inc. Effluent Aquatic Toxicity Report Form - Phase 11 Chronic Ceriodaphnia dubia PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Date: June 27, 2016 Facility: Earth Environmental Services NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee Andrews WWTP Laboratory Performing Test: Environmental Testing Solutions, Inc. Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor: l Comments: Project: 11545 Samples: 160614.07, 160616.07 Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center Raleigh, NC 27699-1621 Sample Information Collection start date: Grab: Composite duration: Alkalinity (mg/L CaCO)): Hardness (mg►L CaCO,): Conductivity (Itmhoslcm): Total residual chlorine (mg/L): Sample Temp. at Receipt (°C): Control Organisms Sample 1 Sample 2 Control 06-13-16 06-15-16 24-h 24.5-h ' 30 40 260 234 151.155.156 <0.10 <0.10 1.2 0.7 Organism Number 2 3 4 5 6 7 8 Test Information Treatment: Initial pH (SU): Final pH (SU): Initial DO (tng/L): Final DO Initial Temp. ("C): Final Temp. ("C): 9 10 11 12 atesn Start date: End date: • Start time: End time: 06-15-16 06-22-16 1230 0703 Stan RetenalI Rcncnsl2 Sun RetcaalI Renewzl2 13% 13% 13% Control Control Control 7.55 7.67 7.50 7.67 7.75 7.81 7.70 7.77 7.61 7.71 7.86 7.56 7.8 7.8 7.8 7.8 7.9 7.7 7.9 8.0 7.8 7.9 8.0 7.9 24.9 25.0 24.8 24.8 24.7 24.7 25.2 25.1 25.0 24.7 24.7 24.7 Number of Young Produced 26 25 25 29 25 26 26 27 28 26 27 26.6 Adult Survival: (L)ive, (D)ead L _29 L L L L L L L L L L , L Effluent Percentage Treatment 2 Organisms 2 3 4 5 6 7 8 9 10 11 12 me. Number of Young Produced 32 29 29 30 27 29 30 28 28 27 29 29 28.9 Adult Survival: (L)ive. (D)ead L L LLL L L L L L L L -8.8 Effluent Percentage Treatment 3 Organisms 2 3 4 5 6 7 8 9 10 11 12 '/. Reduction Number of Young Produced Adult Survival: (L)ive, (D)ead Effluent Percentage 1111 Treatment 4 Organisms 1 ': Reduction 2 3 4 5 6 7 8 9 10 11 12 Ntan Number of Young Produced Adult Survival: (Clive. (D)ead Effluent Percentage Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 s: Reduction Mean Number of Young Produced Adult Survival: (L)ive. (D)ead Effluent Percentage Treatment 6 Organisms 2 3 4 5 6 7 8 9 10 11 12 Reduction Mean Number of Young Produced Adult Survival: (L)ive, (D)ead °,» R W,,niou Overall Analysis: Result: PASS LOEC: >13% NOEC: 13% ChV: >13% Chronic Test Results Final Control Mortality (%): 0.0 %Control with 3rd Broods: 100 Control Reproduction CV: 5.4 48 Hour Mortality Control: 0 of 12 MC: 0 of 1, Significant?: No Final Mortality Significant at: No concentration Reproduction Analyses Reproduction LOEC: >13% Reproduction NOEC: 13% Overall Method: Homosccdastic t Nonnal Distribution: Yes Method: Shapiro-Witk's Statistic: 0.938 Critical Value 0.884 Equal Variances: Yes Method: F-Test Statistic: 1.096 Critical Value: 5.320 Non -Parametric Analysis (if applicable) Method: . Effluent % Rank Stun Critical Sum 13% DWQ form AT--3 (8/91) Rei: 11/95 Test Grouping Information: Facility Project # 6 •a•-tr.•o. 't %ksA-z- 5 tom sew tu..e, 4 Fr A IQ %ksab I (S4 a 3 $ 0 2.t•1Sv tt-Le. 2 Amoces mAS r•' =r; �;1•�•°:-: i.'ir;�'4. .� p." 1- Control Daily Renewal Information: Page 1 of 1 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubla (EPA-821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Control Bench Sheet Control #: ! Date: 06-15-16 Test Organism Information: Day 0 1 2 Date 06-15-16 06-16-16 06-17-16 3 06-18-16 4 5 6 06-19-16 06-20-16 06-21-16 7 06-22-16 Test Initiation, renewal, feeding or termination Time Initiation/Feeding Fee Rene gi ing 'sent, r Analyst Organism Source: in-house Culture Age: < 24-hours old Source (culture board): 014-01-00 A Replicate # 11.1 2 3 4 5 6 7 II1 8 10 11 12 Culture board cup # S �wI . �lb t tS11.9 �I U. -11 Date and time organisms were born between: 04-tS4lo oW E To +z..tO Average transfer volume: 0.0121 ml Transfer bowl information: pH (S.U.): 1, Dtl Temperature rC): - Ls. SSW Batch S.•t24b Ob•ttab Seienastnsm Batch Ywr Batch os-A-1b Location Incubator/Shelf Randomizing Template Sity Feed0tng_, feG Renewal 2/Feeding 3S Feed Temdnauon a'io.5 aW Ob•11•lb 116 Chemical Analyses: Concentration Initiation Initial Final Renewal One Initial Final Control, SSW Analyst pH (S.U.) Dissolved oxygen (mg/L) Conductivity (µmhos/cm) *Alkalinity (mg/L CaCO3) `Hardness (mg/LCaCO3) *Temperature (°C) d` N6 Renewal Two Initial 1.41 -1. e 1St 3 go 7,7/ 7,8 -•75- 7.1 od• ti • 5-t7 1%1.1 451 Io DINcai Final /t 7.5( 7.q rig 4. 'Analyst identified for each day, performed pH, dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation,ILk rwa ortermination by the analyst identified in the Daily Renewal Information table. Alkalinity and hardness performed by the analysts indentified on the test bench sheets and transcribed to this bench sheet. Survival and Reproduction Da Day Observations Replicate number 1 2 3 4 5 6 7 8 9 10 11 12 2 Renewal One Adult mortality (L. Live, D z dead) L L 1✓ L L L L. 5 Renewal Two Number of broods present Number of young produced L, lZ- L l3 L 1 i, I i. It I, L Lk' l._ i l l f 3 t Z i,z IS Lk_ 12_ Liz._ i S Ig 1 b Adult mortality (L a live, 0 e dead) L. —IL L L. L V_ L..L L L_ 7 Final Number of broods present of young produced t't I t t a• l a l. t`-1 L' lb Li t"? L 1 11 I IL L 11:Number li Total young produced b Z- 7-c% 'Ls �-5 -Zel �-S -2!o -2-6 Z"� le � Z1 Final adult mortality (L'Live,Dodead) L..X L. L. L L � L. � L for 3rd Broods x ? X- )< X y /� ) )( $. x X r.,.,rr.,r A.....,.-«,-.-__-..r._--__ %of Male Adults (5 20%) % Adults having 3rd Broods (a 80%) % Mortality (5 20%) f 100. 61, Mean Offspring/Female (k 15 offspring/surviving female) 21640 % CV (< 40%) 6.42 SOP AT12 - Exhibit AT12.3, revision 11-01-14 ti. Environmental Testing Solutions, Inc. Ceriodaphrria dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 - North Carolina Modification Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: Test dales: Project number: Revciwed by: Andrews WWTP June 15-22,2016 11545 Concentration (%) Day Number of young produced by replicate number Survival (%) Average reproduction (offspring/fcmnle) Coefficient of variation (%) Percent reduction from control (%) 1 2 3 4 S 6 7 8 9 10 11 12 Control 5 12 13 lI 11 13 12 11 12 15 12 13 13 100 26.6 5.4 Not applicable 7 14 I6 14 14 16 13 15 14 12 16 13 14 Total 26 29 25 25 29 25 26 26 27 28 26 27 13% 5 16 15 12 15 11 II 15 13 12 12 12 15 100 28.9 4.8 -8.8 7 16 14 17 15 16 18 15 15 16 15 17 14 Total 32 29 29 30 27 29 30 28 28 27 29 29 Dunnctt's f 1SD value: PMSD: 1.445 5.4 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole elpuent toxicity test. Lower PMSD bound determined by USEPA (I0th percentile) = 13%. Upper PMSD bound determined by USEPA (9011' percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200Ia: USEPA, 2001b). USEPA. 200I a, 200lb. Final Report: Interlaboratory Variability Study ol'EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-0-01-004 and EPA-821-13-01-005. US Environmental Protection Agency, Cincinnati. 011. rlrrditcuw 06-15-16 i } t� Environmental Testing Solutions, Inc. Start Date: 6/15/2016 End Date: 6/22/2016 Sample Date: Comments: Ceriodaphnla Survival and Reproduction Test: Reproduction Test ID: CdPFCRNC Sample ID: Andrews VWVTP Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Conc % 1 2. 3 4 5- 6 7 < 8 9 10' D-Control 26.000 29.000 25.000 25.000 29.000 25.000 26.000 26.000 27.000 28.000 D-Control 26.000 27.000 13 32.000 29.000 29.000 30.000 27.000 29.000 30.000 28.000 28.000 27.000 13 29.000 29.000 Transform: Untransformed ' 1-Wed Conc-% Mean N-Mean Mean MIn Max CV% . N t-Stat- Critical MSD 0-Control 26.583 1.0000 26.583 25.000 29.000 5.430 12 13 28.917 1.0878 28.917 27.000 32.000 4.769 12 -4.049 2.508 1.445 Auxiliary Tests Statistic . Critical Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93804 0.884 F-Test indicates equal variances (p = 0.88) 1.09562 5.31963 Skew Kurt 0.62647 -0.1212 Hypothesis Test (1-tail,-0.01) Horn oscedastic t Test indicates no significant differences Treatments vs D-Control MSDu MSDp MSB MSE F-Prob df 1.44544 0.05437 32.6687 1.99242 5.4E-04 1, 22 Andrews 06-15-16 L Environmental Testing Solutions, Inc. Whole Effluent Toxicity Chain -of -Custody Form 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Facility: Earth Environmental Services, Inc. Andrews WWTP Species: Ceriodapltnia dubia Test type: Pass/Fail Chronic NPDES #: NC0020800 Pipe If: 001 County: Cherokee Purchase order: Effluent dilution: 13% Parameter code: TGP3B it Number of samples per hour: Chilled during collection? If chilled, specify temperature: —.— _ ._ems-..._._ .--�-:--�------ --�� �_.. Sample information: (to be completed by sample collector) Composite sample: Sample location: eft-1 -0 'L 4•f Start date: la (r -4/ C Time: �S .l: ti Volume collected for testing: End date: (/ ( Cc-iL[.'O Time: i 801- P/ 1 S a rv� / .I S0� �� f-' Method of transport to laboratory: Number of containers filled for testing: 7 S Comments: Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: rgnatttrc Relinquished by: Mk and time Print Relinquished by: Signature Date and tune Print Signature Mtn and time 1 Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: i4'M't c-Ifx .t (01(4( 0 150S Print Signature Custody seals intact?: )'es Date and time No Not used Samples received in good condition?: J Tracking number: Yes Received by: 1 ><C k Vef Prim Received by: Si nnture I L co(Iw i s r Date and titan K Print Received at ETS by: Siittattac Date and time v %Neal, Print Sienaturc Sample temperature upon receipt at ETS (°C): `o Total residual chlorine upon receipt at ETS: (DPD Presence/Absensc Indicator. MDL s 0.10 tng'L) ‘e-t`l•lb tS( Date and time Present Absent Project number:IMIc Sample number: 10 Oita Comments: 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. Whole Effluent Toxicity Chain -of -Custody Form Facility: Earth Environmental Services, Inc. NPDES #: NC0020800 Pipe #: 001 County: Cherokee Andrews WWTP Purchase order: Species: Cerlodaphizia (labia Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP3S Sample information: (to be completed by sample collector) Composite sample: 1 it Sample location A ,' ` t tJ 0:4 `% �'t�l 1 i is ,. ; e.. �t Start date: a` J( .S f ((J Time: 080 Volume collected for testing: / 1' < 7 End date: (Oft /[ V Time: . o831 1"v Number of containers filled for testing: Number of samples per hour: /S (LI, (e/ 3 o V 0 5 a,L Chilled during collection? 2S Comments: If chilled, specify temperature: / ) Method of transport to laboratory: Per e Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: !YllGht..tf er'yt-cdJ4 G Print Signature Relinquished by: (Midi 3IA; Date and time Received by: Print Relinquished by: Signature Due and time Print Signature Date and tine Received bv: Print Signature Date and time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: �i G( die-tha ('te(r-6 �• 'i Print Custody scats intact?: Ycs No Samples received in good condition?: Tracking number: No: used Vex Ps Date and time N. Print Signature Date and time Received at ETS by: 06.1L' I o -5,.) Maell- i343 # Print Signature Sample temperature upon receipt at ETS (°C): Total residual chlorine upon receipt at ETS: (DPD Presencer,\bseose Indicator. NIDL = 0.10 mg/L.) Date and tune Pram Absent Project number: %Lig Sample number: I I O(o 114.0',- Comments: 7-day IC25 (g/I., NaC1) Environmental Testing Solutions, Inc. 1.I4 I.12 1.10 1.08 1.06 1.04 1.02 2.5 2.0 1.5 1.0 0.5 1.4 1.3 1.2 1.1 1.0 0.9 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart 1 1 1 1 1 1 1 I I 1 1 1 1 I i I I I I I USEPA Control Limits (± 2 Standard Deviations) ...• ........................................................................... I I I I I I I I I IIIIIIIIIIIIIIII USEPA Warning and Control Limits (75rt' and 906 Percentile CVs) I I I I I I I I I I I I I I I I I IIIIIIIIIIIIIIIIIII Laboratory Warning and Control Limits (10`f' and 25th Percentile CVs) I I 1 I I I I 1 I I I I I I I 1 I I I I 0.8 ,. AS 0.3AS . AS 1 S NS •5 N5 N.y • �`' �`' ♦5 �5 �5 �6 �b �b .b 6 0 OZ 01Oa 0=' Os�. OO 09. 01 -�,. O� pq'x' `4 or`�A�' `'LAB 0.." 0,� 09. O.L 0' 03 0`-' 0 — O 0''�0 0 0 0 1 Nb Test date ---c— 7-day IC,5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. -- — • Central Tendency (mean IC25) — — - Warning Limits (mean IC,5 f SA 10 or SA 75) Control Limits (mean IC25 ± SA 25, SA.90, or 2 Standard Deviations) • LnS.red.nd am Swann 1.4 PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. September 23, 2016 Mr. Michael Ladd Earth Environmental Services 75 Bison Lane Murphy, NC 28906 RE: ETS PROJECT NUMBER: 11720 Dear Mr. Ladd: Enclosed are toxicity test results for samples from the Andrews WWTP received by Environmental Testing Solutions, Inc. September 06 through September 08, 2016. Parameter Test Procedure Code EPA Method Final Number Result TGP3B North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure (Ceriodaphnia Pass/Fail Toxicity Test) EPA-821-R-02-013 PASS If this test was performed as an NPDES requirement or by Administrative Letter, please enter a P on the Effluent Discharge Monitoring Form (MR-1) for the collection date September 06, 2016 using the parameter code TGP3B. Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT-3) by October 31, 2016. If you have any questions concerning these results, please feel free to contact me. Sincerely, umner Laboratory Director This report should not be reproduced, except in its entirety, without the written consent of Environmental Testing Solutions, Inc. The results in this report relate only to the samples submitted for analysis. North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600 Sottth Carolina Certificate Number: Clean Water Act: 99053-001 Environmental Testing Solutions, Inc. Effluent Aquatic Toxicity Report Form - Phase II Chronic Ceriodaphnia dubia PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Date: September 23, 2016 Facility: Earth Environmental Services Andrews W1VTP NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee Laboratory Performing Test: Environmental Testing Solutions, Inc. Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor: ,. Comments: Project: 11720 Samples: 160906.06, 160908.07 Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center Raleigh, NC 27699-1621 Sample Information Collection start date: Grab: Composite duration: Alkalinity (mg/L CaCO3): Hardness (mg/L CaCO3): Conductivity (µlnhos/cm): Total residual chlorine (mg/L): Sample Temp. at Receipt ("C): Control Organisms 1 Sample 1 Sample 2 Control 09.05-16 09-07-16 24.5-h 24.5-h 34 40 530 385 161. t60. I.$9 <0.10 <0.10 4.5 0.9 Organism Number 2 3 4 Test Information Treatment: Initial pH (SU): Final pH (SU): initial DO (mg/L): Final DO (mg/L): Initial Temp. ("C): Final Temp. ("C): Start date: End date: Start time: End time: 09-07-16 09-14-16 1010 0802 Stan Renaeal 1 Rama! 2 Stan Rc enal1 Rencnel 2 13% 13% 13% Control Control Control 7.67 7.50 7.59 7.52 7.49 7.53 7.78 7.69 7.63 7.63 7.81 7.64 7.9 7.8 7.9 7.9 7.8 7.9 7.9 8.0 7.9 7.9 8.0 7.9 24.9 24.7 24.9 24.8 24.9 24.8 25.0 24.9 25.1 24.8 24.8 25.0 Number of Young Produced 30 26 27 28 28 29 28 27 27 29 29 30 28.2 Adult Survival: (L)ive, (D)ead L L L LLL L L L L L L Effluent Percentage 13% Treatment 2 Organisms 1 •t Number of Young Produced 32 29 26 28 28 27 29 27_ 29 27 26 30 28.2 Adult Survival: (L)ive, (D)ead L L I. L L L L L L L L L 0.0 Effluent Percentage Treatment 3 Organisms Reduction 2 3 4 5 6 7 8 9 10 11 12 wan Number of Young Produced Adult Survival: (L)ive, (D)ead Effluent Percentage Treatment 4 Organisms Number of Young Produced Adult Survival: (L)ive, (D)ead % Reduction 1 2 3 4 5 6 7 8 9 10 11 12 Atcan Effluent Percentage Treatment 5 Organisms Number of Young Produced Reduction 1 2 3 4 5 6 7 8 9 10 II 12 Mean Adult Survival: (L)ive, (D)ead Effluent Percentage Treatment 6 Organisms 1 % Reduction 2 3 4 5 6 7 8 9 10 11 12 Nlean Number of Young Produced Adult Survival: (L)ive. (D)ead Overall Analysis: Result: PASS LOEC: >13% NOEC: 13% ChV: Reduction >13% Chronic Test Results Final Control Mortality (%): 0.0 %Control with 3rd Broods: 100 Control Reproduction CV: 4.5 48 Hour Mortality Control: 0 of 12 MC.0 of 12 Sitnificant?: No Final Mortality Significant at: No concentration Reproduction Analyses Reproduction LOEC: >13% Reproduction NOEC: 13% Overall Method: Homosccdastic t Normal Distribution: Yes Method: Shapiro -Wick's Statistic: 0.933 Critical Value: 0.884 Equal Variances: Yes Method: F-Test Statistic: 1.906 Critical Value: 5.320 Non -Parametric Analysis (if applicable) Method: Effluent % Rank Sum Critical Stun 13% . D IVO form AT-3 (8/91) Rev. 11/95 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnio dubia Test Grouping Information: Facility Project # 6 1!a pc4 WeSv K.L.e 11i 1to 5 VgAt4Alta t\TZj WW1 WW1/4 4 CC1VM111e. 3 eVtt av1'4 L. 2 f1/41314P 44 11"1? O 1- Control MOON Daily Renewal Information: Page 1 of 1 (EPA-821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Control Bench Sheet Control #: Date: 09-07-16 Test Organism Information: Organism Source: Age: In-house Culture Source (culture board): < 24-hours old Replicate # Culture board cup # Date and time organisms were born between: o8.3q-Ib A 1 2 I 3 I 4 5 6 78 9101112 S to t1 2 I zt 18 O \•fl AD cei --o r000 S$ Average transfer volume: Transfer bowl information: 0.03 mL pH (S.U.): '1. %3 Temperature (°C): 14.1 Day Date Test Initiation, renewal, feeding or termination SSW Batch Selenastrum Batch YWT Batch Location Incubator/Shelf Randomizing Template Time Analyst 0 09-07-16 initlation/Feeding toto 4k tfev. 01.30-k9 ot•ti•tte. 7-CM r r. &LAX. C �t Ky a 1 09-08-16 Feeding -/ -•.e.:U = :.-.,sue 4v141. ; 12''' t 2 09-09-16 Renewal l/Feeding eat o o Y� Vc• II.,II.,-t ,� �'' - .'i• • t - y. .f - ` r r .'•y z•t . Y i i i 'n. t f. 3 09-10-16 Feeding ri : a , t i Sh. is - Y y+, - 3.a! } ,�x ! 'thy Y �-,- t (V d t 3.t 4 09-11-16 Feeding C. ., r . ,- < w SS ► !�l! ,• C it -: >. , 5 09-1z-16 Rene 12/Feedin 0" 20 g Idk t oMo�S-i1o',-.7', t. ' t � E •'ly S t Ct r t 4 r 3.1�ti F t ' f [ ., 6 09-13-16 �fy,(�fr sj Fe`�6n+i V At '" s_. _ , 1 1 r _ 7 09-14-16 Tenni�natjon 8 O r a .. Y :, r tiy, 7 �;a r` _< i s s • Chemical Analyses: Initiation Renewal One Renewal Two Initial Final Initial Final Initial Final Concentration Analyst AlG 44 � A Control, SSW *Analyst pH (S.U.) 7 7.5") 7. e 7 (ta ti 7.g-9. 7-- %y Dissolved oxygen (mg/L) 7.1 7. ej 47.' ',GI 7. Q ?i Conductivity (µmhos/cm) H ._ �� % p }}�;;a atn:If � .;� , ,,, ``, w *Alkalinity (mg/L CaCo3) 3 314 '}? t Mar G 0 10l b 1 'ES " ` '. \ t e:� ' *Hardness (mg/L CaCO3) `,� i --a.. '!:, *Temperature (°C) -2.4i t '?A. f- 14 • � 1A. i 75.0 identified for each day, performed pH, dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation renewal .,.......,;...s.- b ormatton table. Alkalinity and hardness performed by the analysts indentified on the test bench sheets and transcribed to this bench sheet Survival and Reproduction Data: Day 2 Renewal One 5 Renewal Two 7 Final A Observations Adult mortality (t = Uve, D = dead) Number of broods present Number of young produced Adult mortality (L=Live, D=dead) Number of broods present Number of young produced Total young produced Final adult mortality it. .live, D = dead) 1 tta L 30 2 L lZ to LL t1 2(0 L 3 iS 1. L 4 11 Li t1 L X for 3rd Broods Control Acceptance Criteria: % of Male Adults (s 20%) % Adults having 3rd Broods (>_ 80%) Replicate number . 6 7 8 9 10 11 12 L. L, L LLLJL it. 15 IZ. no t 3 is li L L... L- 1--. L l_ L . L 03 L! i3 a l5 L, it l~ 110 tt{ L! i. Z.i. `2. Q 2.1 Z.1 1.9 lei 3D L✓ L L✓ - L L ‘Ixxx>4xx Mean Offspring/Female (2 15 offspring/surviving female) 2e2 r % Mortality (< 20%) • % CV (< 40%) �*57. SOP AT12 - Exhibit AT12,3. revision 11-01-14 ma�cc Tc Page 1of1 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia (EPA-821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Test Bench Sheet Paired with Control # 1 Date: Oct- U%I'o Client Earth Environmental Services Facility Andrews WWTP Project # 1 MO Test Concentration (Chronic Limit) 13.0% Samples were not aerated or treated unless otherwise noted on this form. Control, dilution water and test renewal information are Included on the Control Bench Sheet indicated above. NPDES # NC0020800 Outfall 001 county Cherokee Dilution preparation: mt. Sample ml Dilution water Total volume ml 39 261 300 Chemical Analyses: a Initiation Renewal One Renewal Two Initial Final initial Final Initial Final Concentration Analyst Hi 445 N6 /14, II/4 , 44 Test Concentration pH (S.U.) - -#•.4' 7, 72 7, co r741 7�s- -•63 Dissolved oxygen (mg/L) 7, f %s ,. 7 d d:Q ? 7. 4 Conductivity (µmhos/cm) ' 1, = . *Temperature (°C) 'TA. 4\ -IS • 0 1,,�4-1 -1..A A 1't A is .1: 100% pH (S.U.) 1. 98 ,, ` 7.34 Dissolved oxygen (mg/L) - -7.. q l . 7 �� Conductivity (µmhos/cm) 5 53 Q ` 38s 4 t••,' .f -. a - � �� =4 _ *Total residual chlorine (mg/L) a 0 . IQ - - r . - Sample number Sample 1 lb0°10b.0b Sample 2 1to00t 0�0'1 , , *Analyst identified for each day, performed pH. dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation renewal or terrain •• t'^.. � t the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual Chlorine Bench Sheet and transcribed to this bench sheet. Survival and Reproduction Data (performed at test con Day p se or Observations Replicate number 1 2 3 4 5 6 7 8 9 10 11 12 2 Renewal One Adult mortality O. = live, 0 = dead) L" L L. L L L L. L L L L. L 5 Renewal Two Number of broods present Number of young produced L lb 1:1 1S L, 1' L IL L. 11.. L 12, L 1a L i S L 1 L 13 L 15 L l b Adult mortality (L=Uve,D=dead) L, '- ' ' L ' L L L. L- L L Li i I t 7 Final Test was initiated using Number of broods present Number of young produced L 04) L 1" Li Is L 11_ it. a IS Li 1S L 12. L 11,0 L % %A Li 11 Total young produced 3L 25 2.4. 2.g 2 8 21 -2.4i 21 25 2.1 2( 30 Final adult mortality (L = Live, 0 = dead) Sample 1 Sam le 2 was .......1f I R 1. d L L L k.,. L L L L. L. L enewa s One ( ay 2) and Two (day 5). Samples were diluted to the test concentration prior to use with soft synthetic water and warmed to 25.0 ± 1.0°C in a warm water bath. Comments: Test Results and Statistical Analyses: Test results % Mortality 67, Mean offspring per female �6.2 % Reduction from control Statistics t-Stator Rank Sum o •oro 1-Tailed Critical 2.9)8 PASS or FAIL Figs.3 SOP AT12 - Exhibit ATI2.2, revision 11-01-14 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 - North Carolina Modification Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc. Client: Test dates: Project number: Reveiwed by: Andrews WWTP September 07 - 14, 2016 11720 Concentration (%) Day Number of young produced by replicate number Survival (%) Average reproduction (offspring/female) Coefficient of variation (%) Percent reduction from control (%) 1 2 3 4 5 6 7 8 9 10 11 12 Control 5 16 14 15 17 14 16 15 12 16 13 15 17 100 28.2 4.5 Not applicable 7 I4 12 12 11 14 13 13 15 II 16 14 13 Total 30 26 27 28 28 29 28 27 27 29 29 30 13% • 5 16 15 11 16 12 12 14 15 13 13 15 16 100 28.2 6.2 0.0 7 16 14 15 12 16 15 15 12 16 14 II 14 Total 32 29 26 28 28 27 29 27 29 27 26 30 Dunnett's MSD value: PMSD: 1.564 5.6 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of tcst precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10° percentile) = 13%. Upper PMSD bound determined by USEPA (90`'' percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200Ia: USEPA, 200 Ib). USEPA. 200Ia, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-13-01-004 and EPA-821-13-01-005. US Environmental Protection Agency, Cincinnati, 01-1. ,lndrews 09-07-14 Environmental Testing Solutions, inc. Start Date: 9/7/2016 End Date: 9/14/2016 Sample Date: Comments: Ceriodaphnia Survival and Reproduction Test -Reproduction:, Test ID: CdPFCRNC Sample ID: Andrew WWTP Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Cone%" _ .1 2 3 _ 4 g : 6 7 8 9 10 0-Control 30.000 26.000 27.000 28.000 28.000 29.000 28.000 27.000 27.000 29.000 D-Control 29.000 30.000 13 32.000 29.000 26.000 28.000 28.000 27.000 29.000 27.000 29.000 27.000 13 26.000 30.000 Transform: Untransformed 1-Tailed Conc-% Mean =, '.N-Mean Mean Min Max CV% N t-Stat ' Critical MSD 0-Control 28.167 1.0000 28.167 26.000 30.000 4.499 12 13 28.167 1.0000 28.167 26.000 32.000 6.211 12 0.000 2.508 1.564 Auxiliary Tests Statistic • Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93258 0.884 0.54371 0.305 F-Test indicates equal variances (p = 0.30) 1.90566 5.31963 Hypothesis Test (1-tali, 0.01) MSDu MSDp . MSB MSE F-Prob df Homoscedastic tTest indicates no significant differences 1.56421 0.05553 0 2.33333 1 1. 22 Treatments vs D-Control Andrews 09-07-16 35 I Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. Whole Effluent Toxicity Chain -of -Custody Form Facility: Earth Environmental Services, Inc. Andrews WWTP Species: Ceriodapli:nia dubia Test type: Pass/FaiI Chronic NPDES II: NC0020800 Pipe I: 001 County: Cherokee Purchase order: Effluent dilution: 13% Parameter code: TGP3B Sample information: (to be completed by sample collector) Composite sample: Start date: f) .5) (k Time: End date: ) h )( Time: .S Number of samples per hour: Chilled during collection? If chilled, specify temperature: Sample location: ( 41 Volume collected for testing: f It 1--e e" roc {- Number of containers filled for testing: I Method of transport to laboratory: Comments: Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Simple custody: (to be completed by sample collector and facility personnel) Sample collected by: LaAA Print Relinquished by: Signature Date and time Print Relinquished by: Signature Dale and lime Received by: L Signature nv: ao l umc Received by: Print Signature Date and time Prins z_...=sxxc.;acesr_avCl.«,1.7 .r.-ac-T— ws---^ Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: Print Custody seals intact?: tL Received at ETS by: act' 14 Signature Date and time MM,, l� Not used Samples received in good condition?: Tracking number: Yes MIN No Signature Dam and time 3-Sql-va-e)A, 1,114 AIL• tint Signature Sample temperature upon receipt at ETS (°C): Total residual chlorine upon receipt at ETS: (DPD Presence/Abscnsc Indicator, MDI. = 0.10 mg4.) Project number: i fl W Sam 1ile number: b CP106,0Lo DI, col row y .S C- Prescnl Absent Comments: K i Environmental Testing Solutions, Inc, Whole Effluent Toxicity Chain -of -Custody Form 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Facility: Earth Environmental Services, Inc. Andrews WWTP Species: Ceriodaphnia dubia NPDES #: NC0020800 Pipe #: 001 County: Cherokee Purchase order: Effluent dilution: 13% Test type: Pass/Fail Chronic Parameter code: TGP3B Sample information: (to be completed by sample collector) Composite sample: Start date: End date: CfJsj( Number of samples per hour: Chilled during collection? If chilled, specify temperature: Sample location: f=-,,tcl� KCY t-, Time: t e'''"k Volume collected for testing: Time: IsNumber of containers filled for testing: 1 I Say:\ Method of transport to laboratory: c J / f' Comments: Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: /:Ili C .\� {& 1 Lac1cA Print Relinquished by: cow( )c s iimauuc Date and lime i'— Print Relinquished by: Signature Doc and time Print I Signature Date and time • Received bv: Print Received by: Signature Dale and time — Print Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: 1,681 "; ''_r� Custody seals intact?: tgnalrre Ell No Na used Samples received in good condition?: J Yea Tracking number: Date and Hine No ts Received at EIS by: Signature Dale and lime Pnrd Stgmalurc Sample temperature upon receipt at ETS (°C): Total residual chlorine upon receipt at ETS: (DPI) Presence/Absense Indicator, MDL = 0.10 ntg'L) 09-o�-i�• 1yZt-j Project numher: 1(-}t1,40 Sample number: 1\409 - o - Comments: }'a '1� + 7-day IC25 (g/L NaCI) Environmental Testing Solutions, Inc. 1.14 1.12 1.10 1.08 1.06 1.04 1.02 2.5 2.0 1.5 1.0 0.5 1.4 1.3 1.2 1.0 0.9 0.8 Cerio daphnia dubia Chronic Reference Toxicant Control Chart I I 1 I I I I 1 I I I 1 1 1 1 I 1 1 1 USEPA Control Limits (± 2 Standard Deviations) • ill I I I I I 1 I I I I I I I 1 I USEPA Warning and Control Limits (7511' and 90th Percentile CVs) • • • • • • • ♦ • • • • • • • • • • • • I I I I I I I I t I I I I I ! I I I I I I I I I I I I I I 1 I I I I I I I I I Laboratory Warning and Control Limits (101i' and 25th Percentile CVs) Oa.01,N0 �5.\06p9.'No1,a.'No$01- %, `' `O0� `��,\`�p�.\% `�0��.0�9.�0�0- %No5�0NpS.�Q`�.0001- %1 `,1; 0���0�` -fib Test date • 7-day IC,5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in C'eriocicrphnia reproduction for the test population. Central Tendency (mean IC,5) Warning Limits (mean IC25 ± SA.10 or SA.75) Control Limits (mean IC,5 f SA 25' SA 90, or 2 Standard Deviations) PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. January 03, 2017 Mr. Michael Ladd Earth Environmental Services 75 Bison Lane Murphy, NC 28906 RE: ETS PROJECT NUMBER: 11962 Dear Mr. Ladd: Enclosed are toxicity test results for samples from the Andrews WWTP received by Environmental Testing Solutions, Inc. December 06 through December 08, 2016. Parameter Test Procedure EPA Method Final Code Number Result TGP3B North Carolina Ceriodaphnia Chronic Effluent Toxicity Procedure (Ceriodaphnia Pass/Fail Toxicity Test) EPA-821-R-02-013 PASS If this test was performed as an NPDES requirement or by Administrative Letter, please enter a P on the Effluent Discharge Monitoring Form (MR-1) for the collection date December 06, 2016 using the parameter code TGP3B. Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT-3) by January 31, 2017. If you have any questions concerning these results, please feel free to contact me. Sincerely, Jim Sumner Laboratory Director This report should not be reproduced. except in its entirety. without the written consent of Environmental Testing Solutions. Inc. The results in this report relate only to the samples submitted for analysis. North Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37786, Wastewater: 600 South Carolina Certificate Number: Clean Water Act: 99053-001 PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. Effluent Aquatic Toxicity Report Form - Phase II Chronic Ceriodaphnia dubia Date: January 03, 2017 Facility: Earth Environmental Services Andrews WWTP Laboratory Performing Test: Environmental Testing Solutions, Inc. Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor: NPDES #: NC- 0020800 Pipe #: 001 County: Cherokee Comments: Project: 11962 Samples: 161206.16, 161208.18 Mail Original To: North Carolina Department of Environment and Natural Resources DWQ/ Environmental Sciences Branch 1621 Mail Service Center Raleigh, NC 27699-1621 Sample Information Collection start date: Grab: Composite duration: Alkalinity (mg/L CaCO3): Hardness (mg/L CaCO;): Conductivity (Iunhos/cm ): Total residual chlorine (rng/L): Sample Temp. at Receipt ("C) Control Organisms Sample 1 Sample 2 Control 12-05-16 12-07-16 • 24-h 24-h 32,31,31 40, 40, 42 313 323 155.158.165 < 0.10 <0.10 1.3 0.5 ' Organism Number 2 3 4 5 6 7 8 Test Information Treatment: Initial p11(SU): Final pH (SU): • Initial DO (mg/L): Final DO (mg/L): Initial Temp. (°C): Final Temp. (°C): 9 10 11 12 ht. Stan date: End date: Start time: End time: 12-07-16 12-14-16 1230 0700 Stan Rcnenal 1 Rcncncal 2 Start Rcncral I Reno.' 2 13% 13% 13% Control Control Control 7.27 7.47 7.41 7.22 7.52 7.54 7.53 7.41 7.30 7.47 7.42 7.28 7.8 7.7 7.7 7.8 7.8 7.9 7.9 7.8 7.8 7.9 7.8 7.8 24.8 24.8 24.7 24.8 24 6 24.7 25.1 24.8 25.1 24.9 25.1 25.2 Number of Young Produced 27 30 25 26 28 29 29 26 27 30 27 29 27.8 Adult Survival: (L)ive. (D)ead L L L LLLL L L L L L Effluent Percentage' 13%] Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Number of Young Produced 28 29 26 28 25 29 31 32 28 28 32 32 29.0 Adult Survival: (L)ive, (D)ead L L L L L L L L L L L L -4.5 Effluent Percentage Treatment 3 Organisms 1 % Reduction 2 3 4 5 6 7 8 9 10 11 12 Mean Number of Young Produced Adult Survival: (L)ive, (D)cad Effluent Percentage Treatment 4 Organisms ': Reduction I 2 3 4 5 6 7 8 9 10 11 12 Mean Number of Young Produced Adult Survival: (L)ive, (D)ead Effluent Percentage Treatment 5 Organisms %Reduction 1 2 3 4 5 6 7 8 9 10 11 12 Mean Number of Young Produced Adult Survival: (L)ive, (D)ead Effluent Percentage Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Reduction Mean Number of Young Produced Adult Survival: (L)ive, (D)ead Overall Analysis: Result: PASS LOEC: >13% NOEC: 13% ChV: `» Reduction Chronic Test Results Final Control Mortality (%): 0.0 %Control with 3rd Broods: 100 Control Reproduction CV: 6.0 48 Hour Mortality Control: 0 of 12 IWC: 0 of 12 Significant?: No Final Mortality Significant at: No concentration Reproduction Analyses Reproduction LOEC: >131/. Reproduction NOEC: 13% Overall Method: Ilumoscedastic t Normal Distribution: Yes Method: Shapiro-Wilk's Statistic: 0.952 Critical Value: 0.884 Equal Variances: Yes Method: F-Test Statistic: 1983 Critical Value: 5.320 Non -Parametric Analysis (if applicable) Method: . Emuent °;a Rank Sun Critical Sum 13% . >13% DN'Q form AT-3 (8/91) Ret'. 11/95 Page 1 of 1 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia (EPA-821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Control Bench Sheet Control #: 1 Date: 12-07-16 Test Grouping Information: Facility Project - {# 6 13.44,4i: ttava 5 COVAMWS 4 Ca.,. l FP51b2. 11i0%s-1 ttcwo 3 so& 1 J -.-e. 2 pwoiteuiS t 1.1,6L 1- Control: :`r ±:Y Daily Renewal Information: Test Organism Information: Organism Source: In-house Culture Age: < 24-hours old Source (culture board): tV-19 -'t' P Replicate # _ 1 2 3 4 5 6 7 8 9 10 11 12 Culture board cup # L ` 1 8 ►1 it It 3 2e no 7.1 30 Date and time organisms were born between: 12•61•'b CMS S 113 t.120 Ave'ra'ge transfer volume: 0.03 mL Transfer bowl information: pH (S.U.): 1-$sg Temperature (°C): i.4,S Day Date Test Initiation, renewal, feeding or termination Time Analyst SSW Batch Selenastrum Batch YWT Batch Location Incubator/Shelf Randomizing Template 0 1 2 3 12-07-16 12-08-16 12-09-16 12-10-16 Initiation/Feeding r't3D Fe o2g35- 4 12-11-16 Renewal 1(Ftcding 1 y Feeding t>io.f: 5 6 12-12-16 12-13-16 Feeding Oro! NG RC Re - t\ Z1-041 1 %-So•t o C. 'LC.Z Renewal 2/Feeding vVZ� Feeding t'L-oB441► 7 12-14-16 Termination Chemical Analyses: Initiation Renewal One Renewal Two Initial Final Initial Final Initial Concentration Control, SSW Analyst u6 Final tyC pH (S.U.) Dissolved oxygen (mg/L) Conductivity (µmhos/cm) -I .8 1SS 7Y7 7.1 162. I66 *Alkalinity (mg/L CaCO3) 3Z 31 • l LDS It 7.9 *Hardness (mg/L CaCO3) *Temperature (°C) 0 410 1.`1•l� z5.1 r4 At--; *Analyst identified for each day, performed pH, dissolved oxygen and conductivity measurements only Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table Alkalinity and hardness performed by the analysts indentified on the test bench sheets and transcribed to this bench sheet • )UrVFVUI Ur1U f1CfRvucrc.crvu Day aiar.u. Observations Replicate number 1 2 3 4 5 6 7 8 9 10 11 12 2 Renewal One Adult mortality (L = Live, D = dead) L L_ L. L. L L L- L L L L L-- 5 Renewal Two Number of broods present Number of young produced , iZ- L IS X.iZ 1L L a U.. t' L3' lb 1� L 12 is 1..7 16 Ii I -I Adult mortality (L = Live, 0 = dead) L L L_ L L L L L L- L 7 Final Number of broods present Number of young produced Li. 6i IL L 12- II 1t.k � 1. i kc [. , t S a 1 `2� EL 1 eL- IS a 11 1 1 Li 1 S Total young produced Z\ -LS Zlo 2.,C la Z \ It. -2:1 a Z41 Final adult mortality = Live, 0 =dead) ... L L_ L. L. L t� L L �•- L L.- t� ✓ X� for 3rd Broods � X / /�[`" X /X' >( ?C )( X. )� Control Acceptance Criteria: % of Male Adults (5 20%) m % Adults having 3rd Broods (? 80%) tOcs%. Mean Offspring/Female (>_ 15 offspring/surviving female) 21-8 % Mortality (5 20%) % CV (< 40%) to.0• SOP AT12 - Exhibit AT12.3. revision 11-01-14 North Carolina Chronic Pass/Fail Whole Effluent Toxicity Test, Species: Ceriodaphnia dubia (EPA-821-R-02-013 Method 1002.0, NC Modification — December 2010, Version 3.0) - Test Bench Sheet Client Earth Environmental Services Page 1 of 1 Paired with Control # l Date: l"t• O"1'ik, Facility Andrews WWTP Project # l Nol,_ Test Concentration (Chronic Limit) 13.0% Samples were not aerated or treated unless otherwise noted on this form. Control, dilution water and test renewal information are included on the Control Bench Sheet indicated above. NPDES # NC0020800 Outfall 001 County Dilution preparation: Cherokee ml Sample ml Dilution water Total volume mL 39 261 300 Chemical Analyses: Initiation Renewal One Renewal Two Initial Final Initial Final Initial Final Concentration Analyst / h/6 gv6 j`- llu 01/6 Test Concentration pH (S.U.) 3•27. 7..3 `. 91 0..t-11 7. .?O Dissolved oxygen (mg/L) 7. g 7' 7. 7 1LV 1.1 7.7 Conductivity (µmhos/cm) p 'I S I- ' ` *Temperature (°C) 1,I *8 1.5- k -A. 8 NA. 4 'yt - 1 'ZS • X 1009%o pH (S.U.) 7.28 .i ' 7 .� .- . t' + , Dissolved oxygen (mg/L) -7 et7 r $• , � - 4 Conductivity (µmhos/cm) 313 s. ''s,- 3J ' , ;` . ' ° *.tA' *Total residual chlorine (mg/L) 4 0.‘1) - ' Sample number Sample 1 1V1.06. tI. Sample 2 1 611.0 0 . tQ 'Analyst identified for each day, performed pH, dissolved oxygen and conductivity measurements only. Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on the Control Bench Sheet. Total residual chlorine performed by the analyst identified on the Total Residual Chlorine Bench Sheet and transcribed to this bench sheet. Survival and Reproduction Data (performed at test concentration) : Day Observations Replicate number 1 2 3 4 5 6 7 8 9 10 11 12 2 Renewal One Adult mortality (L = Live, D = dead) L k.... L L '— L L._ L .. 5 Renewal Two Number of broods present Number of young produced �'Y- VA 12.. 1S L la L f 3 1 t' L Ti La._ (S , L IS L L L L 1' La t 1. L 18 Adult mortality (L = Live, D =dead} `- `' ` L L. `" \_ L L L..- ,./4 '34 Final Number of broods present Number of young produced L 14 a 14 iL ‘1.-1 L l S . 12. L 1 Z L l ko L l'1 LL IL L 1b Li IS L i%k Total young produced Zf „ 114 Q C ,�S 'Li 31 32. 'It ,Z& •sZ 3,Z Final adult mortality (L = Live, D = dead) k- L L._ L ,L. Test was initiated using Sample 1. Sample 2 was used for Renewals One (day 2 and Two (days). Samples were diluted to the test concentration prior to use with soft synthetic water and warmed to 25.0 ± 1.0°C in a warm water bath. Comments: Test Results and Statistical Analyses: Test results % Mortality 01. Mean offspring per female 263'o % Reduction from control ' L.i•S")- Statistics t-Stator Rank Sum . I.51Z 1-Tailed Critical Z+� PASS or FAIL piss SOP AT12 • Exhibit AT12.2, revision 11-01-14 Ceriodaphrtia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 - North Carolina Modification Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc. Client: Test dates: Project number: Reveiwed by: Andrews W WTP December 07- 14, 2016 11962 Concentration (%) Day Number of young produced by replicate number Survival (%) Average reproduction (offspring/female) Coefficient of variation (%) l'ercent reduction from control (%) 1 2 3 4 5 6 7 8 9 10 11 12 5 12 15 13 12 12 14 16 13 12 13 16 14 100 • 27.8 6.0 Not applicable Control 7 15 15 12 14 16 15 13 13 15 17 11 15 Total 27 30 25 26 28 29 29 26 27 30 27 29 13% 5 14 15 14 13 13 17 15 15 12 15 17 18 100 29.0 8.1 -4.5 7 14 14 12 15 12 12 16 17 16 13 15 14 Total 28 29 26 28 25 29 31 32 28 28 32 32 Dunnett's MSD value: Pi1SD: 2.074 7.5 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10th percentile) = 13%. Upper PMSD bound determined by USEPA (90`h percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 200Ia. 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-13-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, 01-1. elsulri we 12-07-16 Environmental Testing Solutions, Inc. Ceriodaphnia Survival and Reproduction Test -Reproduction Start Date: 12/7/2016 End Date: 12/14/2016 Sample Date: Comments: Test ID: CdPFCRNC Sample ID: Lab ID: ETS-Envir. Testing Sol. Sample Type: Protocol: FWCHR-EPA-821-R-02-013 Test Species: Andrews VVVVTP DMR-Discharge Monitoring Report CD-Ceriodaphnia dubia Conc% 1 2 3 4 5 6 7 8 9 10 D-Control 27.000 30.000 25.000 26.000 28.000 29.000 29.000 26.000 27.000 30.000 D-Control 27.000 29.000 13 28.000 29.000 26.000 28.000 25.000 29.000 31.000 32.000 28.000 28.000 13 32.000 32.000 Transform: Untransformed 1-Tailed Conc% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD 0-Control 27.750 1.0000 27.750 25.000 30.000 5.976 12 13 29.000 1.0450 29.000 25.000 32.000 8.053 12 -1.512 2.508 2.074 Auxiliary Tests Statistic Critical Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.952 0.884 F-Test indicates equal variances (p = 0.27) 1.98347 5.31963 Hypothesis Test (1-tail, 0.01) Homoscedastic t Test indicates no significant differences Treatments vs D-Control Skew Kurt -0.0618 -0.7378 MSDu MSDp MSB MSE F-Prob df 2.07405 0.07474 9.375 4.10227 0.14484 1,22 indretrs 12-07-16 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. Whole Effluent Toxicity Chain -of -Custody Form Facility: Earth Environmental Services, Inc. Andrews WWTP Species: Ceriodaphnia dubia Test type: Pass/Fail Chronic NPDES #: NC0020800 Pipe #: 001 County: Cherokee Purchase order: Effluent dilution: 13% Parameter code: TGP3B Sample information: (to be completed by sample collector) Composite sample: Start date: End date: ! l b l' Number of samples per hour: Chilled during collection? If chilled. specify temperature: Time: Time: Y {� Sample location:(—tA ._(-S- 1Jeryi Ac kt�K Volume collected for testing: rl (i I Number of containers filled for testing: Method of transport to laboratory: Comments: Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be <6.0°C upon receipt at the laboratory. S'smple custody: (to be completed by sample collector and facility personnel) Sample collected by: PIA-21.C. I1I.6/ ite-agt, Rid Ki13,A4 Print Relinquished by: Signature Date and time Print Relinquished by: T Signature Date and III! Print Signature Date and time Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: La. r �--- / (3 3P Print Signature Date and bare Custody seals intact?: YiN Samples received in good condition?: 'tracking number: \o nut used I ...\1I Yes s Received by: /� 744 / yt / Pnnt Iteceired by: Print Recciveti t ETS bv: Signature Signature /at-v.. 1 /4, f2 O'° t 1—-A (/- 133 e Pint Signature Sample temperature upon receipt at ETS CC): Total residual chlorine upon receipt at ETS: (DPD Presence:Absense Indicator. MDL = 0.10 mg'L) ale and trnK r.3c, Present ✓I Al.sent Project number: I (a1(/Z Sample number: l "PIZU(a.I(p Comments: ri Environmental Testing Solutions, Inc. �e - r�(.7-`�� Whole Effluent Toxicity Chain -of -Custody Form 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 Facility: Earth Environmental Services, Inc. Andrews WWTP Species: Ceriodaphnia dubia Test type: Pass/Fail Chronic NPDES #: NC0020800 Pipe #: 001 County: Cherokee Purchase order: Effluent dilution: 13% Parameter code: TGP3B wenzetawrar Sample information: (to be completed by sample collector) Composite sample: Start date: tafk.("' End date: i .. -/ Q' Number of samples per hour: Chilled during collection? If chilled, specify temperature: Time: �S.es4i�QvL, Time:Cg' Sample location'! !t f C-41v Volume collected for testing: / I) Number of containers filled for testing: / �lFz� Method of transport to laboratory: Comments: Eg S Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) Sample collected by: 11��PKl' fimirL6/ Print Relinquished by: Signature /.2/n'I/4; Date and time Prim Relinquished by: Signature Date and time Print Signaturc Dale and time Received by: Print Signature Received by: Date and tines Print Signature Dale and once Esc+.._. sv.,.� �sissa.vs-+ov1s.�.nrn.r..s._c.rrn-unawurmmx.�rars�v..-asav,..msww+exnwaw+ Sample receipt information: (to be completed by ETS personnel) Relinquished to ETS by: 0'1'rc.6tc- Print Custody seals intact?: Signature Yes Nu Samples received in good condition?: Tracking number: /-1/// ,,Z5 3 " Nut used Date and time Received at ETS be: Sample temperature upon receipt at ETS (°C): Total residual chlorine upon receipt at ETS: (DIM Presence'Absensc Indicator. MDL = 0.10 mg I.I ate and lime 0.5L Present Absent Project number: ! \"110 - Sample number: r'44012_ 4 Cr Comments: Environmental Testing Solutions, Inc. 1.14 1.12 1.10 1.08 1.06 1.04 1.02 2.5 1.5 1.0 0.5 Ceriodaphtzia dubia Chronic Reference Toxicant Control Chart I I I • I i I I I I I I II I I I I I USEPA Control Limits (± 2 Standard Deviations) I I I I I I I I I I I I I I I I I I I I I I I I I USEPA Warning and Control Limits (75t11 and 90th Percentile CVs) •— —• • • • --• --• • • • •-- •--•--• -• — • - • - --• I I I I I I I I I I I I I I Laboratory Warning and Control Limits (10th and 2517 Percentile CVs) •—•—•_-_•_•_• • 0.8 I I I I I I I I I I I I I I I I A \�' ,os•5 \5 0', \5 \5 \� \� 9 \6 \b 5 \6 5 \b 0 \b 10 L \6 1,0 3 \b \ 0 \ 0 b 0 0�,\ 0� ' 00.\ ' \0 0 ' \\ 0�" \ti0a" 0\.\ti' OZ 09' Oti 0 ' O.s. "OVA " 0\A ' Oq.\ ' Ob 0 ' 0,\ ' OaA ' Oq,\ \0 0 \\ 0 \ti.0 Test date • 7-day IC,{ = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC,5) Warning Limits (mean IC25 ± SA to or SA 75) Control Limits (mean IC25 ± SA 25, SAA, or 2 Standard Deviations) .( merte.ee ■.r ewM by Ilm Sumn • • t A6 opt. �.r_: r 2:b 740 r r • 1 Quad: Andrews, N.C. Subbasin: 040502 Latitude: 35°11'51" Longitude: 83°50'46" Receiving Stream: Valley River Stream Class: C-Trout HUC#: 06020002 NC0020800 Town of Andrews WWTP I \ ` . t t r 1� . SCALE 1:24000 EN:SING YA*KYE TOP 11�! 171%9+' IN14:11T ELEV. 1707.7v' (.1 rtr s• P 8" I:KA�111' VC.) EXISTING SLUDGE DRYING arcs • CONC. ' EX/STING • SWDGE DRYING BEDS EXISflNC u/UINCIC _•< 1 TOP EL 1716.29' • - DIP INV EL 171169'• Y N r • PVC. 7t trCIP a+sTlN UANP. t,e .«. 1 TCs, EL 171404' I r to- +NT. Et. 1712.11J' r 12' C. V. aN I BOX COOING YANI•al TOP EL, 1717.91' ON CL 1712.81' (('f'1 •T < \` E3ISTiNG HE, V CF S c � .o` / 61. 0 ASPriALT PAVING ------'..N Annual Monitoring and Pollutant Scan Permit No. K.4-001.0 Outfall ) Month kK-✓ Year �a_i` j L{L{ Facility Name: Town of Andrews WWTP ORC: Michael Ladd Date of sampling: 9/12/2014 Phone: 828-321-3006 Analytical Laboratory: Earth Environmental Services, Environmental Testing Solutions, Rogers and Callcott Parameter Sample Type Analytical Method Quantitation Level Sample Result Units of Measurement Number samples Ammonia as NI Composite SM4500NH3F 0.1 6.8 mg/I Dissolved oxygen Composite SM4500-0 G 0.1 7.4 mg/I Nitrate/ Nitrite Composite SM4500NO3-H 0.20 1.72 mg/ 1 Total Kjeidahl nitrogen Composite 351.2 0.20 9.34 mg/I Total Phosphorus Composite 200.7 0.020 2.6 mg/I Total dissolved solids Composite SM2540 C 1.0 323.0 Hardness Composite SM2340 B 0.66 77 mg/I Chlorine (total residual. TRC) Grab SM 4500 Cl G 0.010 0.011 mg/I Oil and grease Grab 1664 A 5.0 ND mg/I Antimony Composite 200.8 5.0 ND ug/ 1 Arsenic Composite 200.8 10.0 ND ug/1 Beryllium Composite 200.8 1.0 ND ug/1 (:ndmium Composite 200.8 1.0 ND ug/ 1 Chromium Copper Lead Composite Composite 200.8 200.8 5.0 1.0 ND 9.0 ug/1 ugh Composite 200.8 5.0 ND ugh Mercu n Composite 200.8 0.2 ND ug/I Nickel Composite 200.8 10.0 ND ug/1 Selenium Composite 200.8 10.0 ND ug/1 Silver Composite 200.8 5.0 ND ug/ I Thallium Composite 200.8 0.5 ND ug/I Zinc Composite 200.8 10.0 46 ug/I Cyanide Total phenolic compounds Grab Grab SM4500 CN E 420.1 0.005 0.005 ND 0.009 mg/ 1 mg/1 Volatile organic compounds Acrolein Grab 624 5.0 ND ug/1 Acrvlonitrile Grab 624 5.0 ND ug/1 Benzene Grab 624 2.0 ND ug/1 Bromoform Grab 624 2.0 ND ug/1 Carbon tetrachloride Grab 624 2,0 ND ug/1 Chlorobenzene Grab 624 2.0 ND ug/1 Chlorodibromomethane Grab 624 2.0 ND ug/I Chlorocthanc Grab 624 2.0 ND ug/l 2-chlorocthylvinyl ether Chloroform Grab Grab 624 624 5.0 2.0 ND 2.7 ugh l ug/ 1 Dich lorohromomcthanc Grab 624 2.0 ND ug/ I 1.1-dichloror.thane Grab 624 2.0 ND ug/1 1 .2-dichloroetbane Grab 624 2.0 ND ug/1 Trans- 1 .2-die hloroet hylenc Grab 624 2.0 ND ugh 1 Form - DMR- PPA-1 Page 1 Annual Monitoring and Pollutant Scan Permit No. i1 C V ti 1C $ C Outfall 0 0 Parameter Sample Type Analytical Method Quantitatlon Level Sample Result Units of Measurement Number c samples Volatile organic compounds (Cont.) 1.1-dichloroethylene Grab 624 2.0 ND ug/l 1 1,2-dichloropropane Grab 624 2.0 ND ug/1 1 1,3-dichloropropylcne Grab 624 2.0 ND ug/1 1 Ethylbenzene Grab 624 2.0 ND ug/1 1 Methyl bromide Grab 624 2.0 ND ug/I 1 Methyl chloride Grab 624 2.0 ND ug/1 1 Methylene chloride Grab 624 2.0 ND ug/1 1 1,1,2,2-tetrachloroethanc Grab 624 2.0 ND ug/1 1 Tetrachloroethylene Grab 624 2.0 ND ug/1 1 Toluene Grab 624 2.0 ND ug/1 1 1,1,1-trichloroethane Grab 624 2.0 ND ugh 1 1.1.2-trichloroethane Grab 624 2.0 ND ug/1 l Trichloroethylene Grab 624 2.0 ND ug/1 1 Vinyl chloride Grab 624 2.0 ND ug/1 _ 1 Acid -extractable compounds P-chloro-m-crcao Grab 625 5.00 ND ug/1 1 2-chlnrophenol Grab 625 5.00 ND ug/1 1 2.4-dichlnmphenol Grab 625 5.0U ND ug/1 1 2,4-dimethylphanal Grab 625 5.00 ND ug/I 1 4,6-dinitro-o-cresol Grab 625 5.00 ND ugh 1 2,4-d niu•ophenol Grab 625 5.00 ND ug/1 1 2-niu-ophenol Grab 625 5.00 ND ugh 1 4-nitrophenol Grab 625 5.00 ND ug/1 1 Pentuehloruphenol Grab 625 5.00 ND ug/I 1 Phenol Grab 625 5.00 ND ugh 1 2,4,6-trichloruphenol Grab 625 5.00 ND ug/1 1 Base -neutral compounds Accnaphthcne Grab 625 5.00 ND ug/1 1 Accnaphthylcnc Grab 625 5.00 ND ug/1 1 Anthracenc Grab 625 5.00 ND ug/1 1 Bcnzidine Grab 625 80.0 ND ug/1 1 Bcnzotalanthraccnc Grab 625 5.00 ND ug/1 1 lienzola►pyrene Grab 625 5.00 ND ug/I 1 3,4 benzotluoranthene Grab 625 5.00 ND ug/1 1 Benzoighi)perylene Grab 625 5.00 ND ug/1 1 Benzo(k)fluoranthene Grab 625 5.00 ND ug/I 1 Bis (2-chloroethoxyl methane Grab 625 5.00 ND ugh 1 Bis (2-chloroethyl) ether Grab 625 5.00 ND ugh 1 Bis (2-chloroisopropyl) ether Grab 625 5.00 ND ug/1 1 Bis 12-cthylhexyl) phthalate Grab 625 5.00 28.7 ug/1 1 4-bromophenyl phenyl ether Grab 625 5.00 ND ug/1 1 Butyl henry' phthalate Grub 625 5.00 ND ug/1 1 2-chloronaphthalenc Grab 625 5.00 ND ug/1 t• 1 4-chlorophenyl phenyl ether Grab 625 5.00 ND ug/1 1 Form - DMR- PPA-1 Page 2 Annual Monitoring and Pollutant Scan Permit No. DJC DOJCD u Outfall D l: Month S1"ylwl (Ire Year 1 (-f Parameter Sample Type Analytical Method Quantitation Level Sample Result Unite of -Measurement. Number p samples Base -neutral compounds (cont.) Chtysene Grab 625 5.00 ND ug/1 1 Di-n-butyl phthalate Grab 625 5.00 ND ug/1 1 Di-n-octvl phthalate Grab 625 5.00 ND ug/1 1 Dibenzola.h)anthracene Grab 625 5.00 ND ug/1 1 1.2-dichlorobenzene Grab 625 5.00 ND ug/1 1 1,3-dichlorobenzene Grab 625 5.00 ND ug/1 1 1.4-dichlorobenzene Grab 625 5.00 ND ug/1 1 3,3-dichlorobenzidinc Grab 625 5.00 ND ug/1 1 Diethyl phthalate Grab 625 5.00 ND ug/1 1 Dimethyl phthalate Grab 625 5.00 ND ug/I 1 2,4-dinitrotoluene Grab 625 5.00 ND ug/1 1 2.6-dinitrotoluene Grab 625 5.00 ND ug/1 1 1.2-diphenylhydrazine Grab 625 5.00 ND ug/1 1 Fluoranthene Grab 625 5.00 ND ug/I 1 Fluorenc Grab 625 5.00 ND ug/1 1 Hexachlorobenzene Grab 625 5.00 ND ug/1 1 Hexachlorobutadiene Grab 625 5.00 ND ug/1 1 Hexachlorocyclo-pcntadicnc Grab 625 5.00 ND ug/1 1 Hexachloruethanc Grab 625 5.00 ND ug/1 1 Indeno(1,2,3-cd)pyrene Grab 625 5.00 ND ug/1 1 Isophoronc Grab 625 5.00 ND ug/1 1 Naphthalene Grab 625 5.00 ND ug/1 1 Nitrobenzene Grab 625 5.00 ND ug/1 1 N-nitrosucii-n-pmpylamine Grab 625 5.00 ND ug/1 1 N-nitrosudimethylaminc Grab 625 5.00 ND ug/1 1 N-nttrosodiphenylamine Grab 625 5.00 ND ug/I 1 Phenanthrene Grab 625 5.00 ND ug/1 1 Pyrenc Grab 625 5.00 ND ug/1 1 1.2.4,-lnchfur obenzene Grab 02P a.0U ND ug/I 1 I certify under penalty of law that this document and all attachments were prepared under my direction and supervision in accordance with a system to design to assure that qualified perdonnel properly gather and evaluat the information submitted. Based on my inquiry of the person or persons that manage the system, or those persons directly responsibel for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate and complete. I am aware that there arc significant penalties for submitting false information, including the possibility of fines and imprisonment for knowing violations. t -I Authorized. pres tativelime kt Signature CPPLI Date Form - DMR- PPA-1 Page 3 Permit No. A'1LU% Outfall 0 01 Annual Monitoring and Pollutant Scan Facility Name: Town of Andrews WWTP Date of sampling: 12/8/2015 ORC: Tim Wood Phone: 828-321-3006 Month � C -C? 04 1x-✓ Year J C (- Analytical Laboratory: Earth Environmental Services, Environmental Testing Solutions, Rogers and Cancan Parameter Sample Type Analytical Method Quantitation Level Sample Result Unita of Measurement Number u samplea Ammonia las NI Composite SM4500NH3F 0.1 3.0 mg/1 1 Dissolved oxygen Composite SM4500-0 G 0.1 7.8 mg/1 1 Nitrate/Nitrite Composite SM4500NO3-H 0.10 1.18 mg/1 1 Total Kjcldahl nitrogen Composite 351.2 0.10 6.74 mg/1 1 Total Phosphorus Composite 200.7 0.010 1.0 mg/I 1 Total dissolved solids Composite SM2540 C 1.0 170.0 mg/1 1 Hardness Composite SM2340 B 1.0 76 mg/1 1 Chlorine (total residual. TRC) Grab SM4500CLG 0,010 ND mg/1 1 Oil and grease Grab 1664 A 5.0 ND mg/1 1 Antimony Composite 200.8 5.0 ND ug/1 1 Arsenic Composite 200.8 10.0 NO ug/I 1 Beryllium Composite 200.8 1.0 ND ug/1 1 Cadmium Composite 200.8 1.0 ND ug/1 1 Chromium Composite 200.8 5.0 ND ug/1 1 Copper Composite 200.8 2.0 5.0 ugh 1 Lead Composite 200.8 5.0 ND ug/l I. Mercury Composite 245.1 0.2 ND ugh 1 Nickel Composite 200.8 10.0 ND ug/1 1 Selenium Composite 200.8 10.0 ND ug/1 1 Silver Composite 200.8 5.0 ND ug/1 1 Thallium Composite. 200.8 0.5 ND ug/1 1 Zinc Composite 200.8 10.0 32 ug/1 1 Cyanide Grab sM4snn C.N.F. 0.005 0.005 mg/1 1 Total phenolic compounds Grab 420.1 0.005 ND mg/1 1 Volatile organic compounds Acrolein Grab 624 5.0 ND ug/1 I Acrylonitrile Grab 624 5.0 ND ug/1 1 Benzene Grab 624 2.0 ND ug/I 1 Bromoforrr. Grab 624 2.0 ND ug/I 1 Carbon tetrachloride Grab 624 2.0 ND ug/I 1 Chlorobrozene Grab 624 2.0 ND ug/1 1 Chlorodibromomethane Grab 624 2.0 ND ug/1 1 Chloroethane Grab 624 2.0 ND ug/1 1 2-chloroethylvinyl ether Grab 624 5.0 ND ug/1 1 Chloroform Grab 624 2.0 2.5 ug/1 1 Dichlorobromomethane Grab 624 2.0 ND ug/1 1 1.1-dichloroethane Grab 624 2.0 ND ug/I 1 1.2-dichloroethanc Grab 624 2,0 ND ug/1 , 1 Trans-1.2-diehloroethylene Grab 624 2.0 ND ug/1 1 Form - DMR- PPA-1 Page 1 Annual Monitoring and Pollutant Scan Permit No. hJCGG �LSSiiv Outfall t1C Month el"��e�' Year Parameter Sample Type Analytical Method Quantitation Level Sample Result Units of Measurement Number c samples Volatile organic compounds (Cont.) 1,1-dichloroethylene Grab 624 2.0 ND ug/1 1 1,2-dichloropropane Grab 624 2.0 ND ugh 1 1.3-dichloropropylene Grab 624 1.0 ND ug/1 1 Ethylbenzene Grab 624 2.0 ND ug/1 1 Methyl bromide Grab 624 2.0 ND ug/1 1 Methyl chloride Grab 624 2.0 ND ug/I 1 Methylene chloride Grab 624 2.0 ND ug/1 1 I ,1,2,2-trtrachlorocthanc Grab 624 2.0 ND ugh l 1 Tetrachlornethylene Grab 624 2.0 ND ug/1 1 Toluene Grab 624 2.0 ND ug/1 1 1.1,1-trichloroethane Grab 624 2.0 ND ug/1 1 1.1,2-trichloroethane Grab 624 2.0 ND ug/1 1 Trichloroethylene Grab 624 2.0 ND , ugh 1 Vinyl chloride Grab 624 2.0 ND ugh! I Acid -extractable compounds P-chloro-m-creso Grab 625 5.00 ND ug/l 1 2-chlorophenol Grab 625 5.00 ND ug/1 1 2,4-dichlorophenol Grab 625 5.00 ND tig/1 1 2,4-dimerhylphcnol Grab 625 5.00 ND ug/1 1 4,6-dinitro-o-cresol Grab 625 5.00 ND ug/1 1 2.4-dinitrophenol Grab 625 20.0 ND ug/1 1 2-nitrophenol Grab 625 5.00 ND ug/1 1 4-nitrophcnol Grab 625 5.00 ND ug/1 1 Pentachloruphenol Grab 625 10.0 ND ug/1 1 Phenol Grab 625 5.00 ND ug/1 1 2,4,6-trichlorophenol Grab 625 5.00 ND ug/1 1 Base -neutral compounds Acenaphthene Grab 625 5.00 ND ug/1 1 Acenaphthylenc Grab 625 5.00 ND ug/1 1 Anthracene Grab 625 5.00 ND ug/1 1 Benzidine Grab 625 80.0 ND ug/1 1 Benzo(alanthracene Grab 625 5.00 ND ug/1 1 Benzo(a)pyrcne Grab 625 5.00 ND ug/1 1 3,4 henzofluoranthene Grab 625 5.00 ND ug/l 1 Benzo(ghi)perylcnc Grab 625 5.00 ND ugh 1 Benau(k)fluoranthcne Grab 625 5.00 ND ug/1 1 Bis (2-chloroethoxy) methane Grab 625 5.00 ND ug/1 1 Bis (2-chloroethyll ether , Grab 625 5.00 ND ug/1 1 Bis (2-chloroisoprupyl) ether Grab 625 5.00 ND ug/1 1 Bis (2-ethylhexyl) phthalate Grab 625 5.00 6.88 ug/l 1 4-bromophenyl phenyl ether Grab 625 5.00 ND ugh 1 Butyl bcnzyl phthalate Grab 625 5.00 ND ug/l 1 2-chloronaphthalene Grab 625 5.00 ND ug/1 l 1 4-chlnrophcnyl phenyl ether Grab 625 5.00 ND ug/1 1 Form - DMR- PPA-1 Page 2 �,� Annual Monitoring and Pollutant Scan Permit No. Outfall L' .i Month AA? C -a „t4 ba/. Year 2: Parameter Sample Type Analytical Method Quantitation Level Sample Result Units of Measurement Number t sample) Base -neutral compounds 1cont.) Chrvsenc Grab 625 5,00 ND ug/1 1 Di-n-butvl phthalate Grab 625 5.00 ND ug/1 1 Di-n-octyl phthalate Grab 625 5.00 ND ug/1 I Dibenzola.hlanthracene Grab 625 5.00 ND ug/1 1 1,2-dichlorobenzene Grab 625 5.00 ND ug/1 1 1.3-dichlorobenzene Grab 625 5.00 ND ug/1 1 1.4-dichlorobenzene Grab 625 5.00 ND ug/1 1 3,3-dichlorobenzidine Grab 625 5.00 ND ug/1 1 Diethyl phthalate Grab 625 5.00 ND ug/1 1 Dimcthyl phthalate Grab 625 5.00 ND ug/1 1 2.4-diniuotoluenc Grab 625 5.00 ND ug/1 1 2,6-dinttrotoluene Grab 625 5.00 ND ug/1 1 l.2-diphcnylhydrazine Grab 625 5.00 ND - ugh 1 Fluoranthcnc Grab 625 5.00 ND ug/1 1 Fluorene Grab 625 5.00 ND ug/1 1 Hexachlorobenzene Grab 625 5.00 ND ug/1 1 Hcxachlorobutadiene Grab 625 5.00 ND ug/1 1 Hexuchlurocyclo-pentadiene Grab 625 5.00 ND ug/1 1 Hexachlorocthanc Grab 625 5.00 ND ug/1 1 Indenn11.2.3-cdlpyrcnc Grab 625 5.00 ND ug/l 1 lsophurunc Grab 625 5.00 ND ug/1 1 Naphthalene Grab 625 5.00 ND ug/1 1 Nitrobenzene Grab 625 5.00 ND ug/1 1 N-nitrosodi-n-propylaminr Grab 625 5.00 ND ug/1 1 N-nitrosxlimethylamine Grab 625 5.00 ND ug/1 1 N-nitrosodiphcny-lamine Grab 625 5.00 ND ug/1 1 Phenanthrene (:rnh 625 5.00 ND ug/1 1 Pvrene Grab 625 5.00 ND ug/1 1 1,2,4,-trtchlorobenzene Grab 625 J.UU Ni) ugh) I I certify under penalty of law that this document and all attachments were prepared under my direction and supervision in accordance with a system to design to assure that qualified perdonnel properly gather and evaluat the information submitted. Based on my inquiry of the person or persons that manage the system, or those persona directly responsibel for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fines and imprisonment for knowing violations. Form - DMR- PPA-1 Tun wood Authorized Representative name Si ature Date r. Page 3 Annual Monitoring and Pollutant Scan Permit No. % JC.GGav Outfall G " I Month /'' Year C 1 ‘to Facility Name: Town of Andrews WWTP ORC: Tim Wood Date of sampling: 3/8/2016 Phone: 828-321-3006 Analytical Laboratory: Earth Environmental Services, Environmental Testing Solutions, Rogers and Callcott Parameter Sample Type Analytical Method Quantitation Level Sample Result Units of Measurement Number t sample. Ammonia las N) Composite SM4500NH3F 0.1 3.7 mg/I 1 Dissolved oxygen Composite SM4500-0G 0.1 7.7 mg/I 1 Nitrate/Nitrite Composite SM4500NO3-H 0.10 3.02 mg/I 1 Total Kjeldahl nitrogen Composite 351.2 0.10 4.90 mg/1 1 Total Phosphorus Composite 200.7 0.050 1.6 mg/1 I Total dissolved solids Composite SM2540 C 1.0 167.0 mg/1 1 Hardness Composite SM2340 C 1.0 73 mg/I I Chlorine (total residual. TRCI Grab SM 4500 CL G 0.010 ND mg/1 1 Oil and grease Grab 1664 A 5.0 ND mg/I 1 • Antimony Composite 200.8 5.0 ND ug/1 1 Arsenic Composite 200.8 10.0 ND ug/1 1 Beryllium Composite 200.8 1.0 ND ug/l 1 Cadmium Composite 200.8 1.0 ND ug/1 1 Chromium Composite 200.8 5.0 ND ug/1 1 Copper Composite 200.8 2.0 5.0 ug/1 1 Lead Composite 200.8 5.0 ND ug/1 1 Mercury Composite 245.1 0.2 ND ug/1 1 Nickel Composite 200.8 10.0 ND ug/l 1 Selenium Composite 200.8 10.0 ND ug/1 1 Silver Composite 200.8 5.0 ND ug/1 1 Thallium Composite 200.8 0.5 ND ug/1 1 Zinc Composite 200.8 10.0 24 ug/1 1 Cyanide Grab SM4500 Cti E 0.005 0.005 mg/1 1 Total phenolic compounds Grab 420.1 0.005 ND mg/1 1 Volatile organic compounds Acrolein Grab 624 5.0 ND ug/1 1 Acrvlonitrilc Grab 624 5.0 ND ughl 1 Benzene Grab 624 2.0 ND ug/1 1 Bromoform Grab 624 2.0 ND ug/1 1 Carbon tetrachloride Grab 624 2.0 ND ug/1 1 Chlorobenzene Grab 624 2.0 ND ug/1 1 Chlorodibromomethane Grab 624 2.0 ND ug/I 1 Chlorocthane Grab 624 2.0 ND ug/1 1 2-chloroethylvinyl ether Grab 624 5.0 ND ug/1 1 Chloroform Grab 624 2.0 ND ug/1 1 I)inhlnrohromomcthane Grab 624 2.0 ND ugh 1 1,1-dichlornethanc Grab 624 2.0 ND ug/1 1 1.2-dichloroethane Grab 624 2.0 ND ug/1 r. 1 Trans-l.2-dichloroethylene Grab 624 2.0 ND ug/1 `' 1 Form - DMR- PPA-1 Page 1 /J G G G �Gg 0 C Annual Monitoring and Pollutant Scan Permit No. Outfall G O I Month 1► arC-1 Year -Z0 l Parameter Sample Type Analytical Method Quaantitation Level Sample Result Units of Measurement Numbet, gyp* Volatile organic compounds (Cont.) 1,1-dichloroethylene Grab 624 2.0 ND ug/I 1 1,2-dichloropropane Grab 624 2.0 ND ug/1 1 1,3-dichloropropylene Grab 624 1.0 ND ug/1 1 Ethylbenzene Grab 624 2.0 ND ugh! 1 Methyl bromide Grab 624 2.0 ND ug/1 1 Methyl chloride Grab 624 2.0 ND ug/1 1 Methylene chloride Grab 624 2.0 ND ug/1 1 1,1,2,2-tetrachlorocthanc Grab 624 2.0 ND ug/1 1 Tetrachloroethylene Grab 624 2.0 ND ug/1 1 Toluene Grab 624 2.0 ND ugh 1 1.1,1-trichloroethane Grab 624 2.0 ND ugh! 1 1,1,2-trichloroethane Grab 624 2.0 ND ug/1 1 Trichloroethylene Grab 624 2.0 ND ug/1 1 Vinyl chloride Grab 624 2.0 ND ug/1 1 Acid-eatraatable compounds P-chloro-m-creso 2-chlorophenol 2,4-dichlorophenol Grab Grab Grab 625 625 625 5.00 5.00 5.00 ND ND ND ug/1 ug/1 ug/ I 2,4-dimethylphenol 4,6-dinitro-o-cresol 2,4-d i n itrophenol 2-nitrophenol 4-nitrophenol Grab Grab Grab Grab Grab 625 625 625 625 625 5.00 5.00 20.0 5.00 5.00 ND ND ND ND ND ug/1 ug/ I ug/I ug/1 ug/1 Pentachlorophenol Phenol 2,4,6-trich torophenol Grab Grab Grab 625 625 625 10.0 5.00 5.00 ND ND ND ug/I ug/I ug/1 1 1 1 1 1 1 1 1 1 Base -neutral compounds Acenaphthene Grab 625 5.00 ND ug/1 Acenaphthylene Grab 625 5.00 ND ug/1 Anthracene Benzidine Benzo(a)anthracene Grab Grab Grab 625 625 625 5.00 80.0 5.00 ND ND ND ug/1 ug/1 ug/1 Benzola►pyrcne Grab 625 5.00 ND ug/ 1 3.4 benzofluoranthenc Benzolghi)perylene Benzo(k)fluoranthene Grab Grab Grab 625 625 625 5.00 5.00 5.00 ND ND ND ug/1 ugh 1 ug/I Bis (2-chloroethoxy) methane Grab 625 5.00 ND ug/I Bis 12-chloroethyl) ether Grab 625 5.00 ND ug/I Bis (2-chloroisopropyl) ether Grab 625 5.00 ND ug/1 Bis (2-ethythexyl) phthalate Grab 625 5.00 5.11 ug/1 4-bromophenyl phenyl ether Butyl bcnzyl phthalate 2-chloronaphthalcnc 4-chlorophenyl phenyl ether Grab Grab Grab Grab 625 625 625 625 5.00 5.00 5.00 5.00 ND ND ND ND ug/1 ug/1 ug/1 ^ ug/ I 1 1 1 1 1 1 1 Form - DMR- PPA-1 Page 2 C GCS C� Annual Monitoring and Pollutant Scan Permit No. Outfall 0 u Month n��"C• 11 Year ) Parameter Sample Type Analytical Method Quantitation Level Sample Result Units of Measurement Number sample Rase -neutral compounds (cont.) Chrysene Grab 625 5.00 ND ug/1 1 Di-n-butyl phthalate Grab 625 5.00 ND ug/1 1 Di-n-octyl phthalate Grab 625 5.00 ND ug/1 1 Dibenzo(a.hlanthracene Grab 625 5.00 ND ug/1 1 1,2-dichlorobenzene Grab 625 5.00 ND ug/1 1 1,3-dichlorobenzene Grab 625 5.00 ND ug/1 1 1.4-dichlorobenzene Grab 625 5.00 ND ug/1 1 3,3-dichiurobenzidine Grab 625 5.00 ND ug/I 1 Diethyl phthalate Grab 625 5.00 ND ug/1 1 Dimethyl phthalate Grab 625 5.00 ND ug/1 1 2,4-dinitrotoluene Grab 625 5.00 ND ug/1 1 2,6-dinitrotoluene Grab 625 5.00 ND ugh' 1 1,2-dipherwlhvdrazine Grab 625 5.00 ND ug/1 1 Fluoranthene Grab 625 5.00 ND ugh 1 Fluorene Grab 625 5.00 ND ug/1 1 Hexachlorobenzene Grab 625 5.00 ND ugh 1 Hexachlorobutadienc Grab 625 5.00 ND ug/1 1 Hexachlorocvclo-pcntadiene Grab 625 5.00 ND ug/1 1 Hexachloroethane Grab 625 5.00 ND ug/1 1 lndcnol1.2.3-cd)pyrene Grab 625 5.00 ND ugh 1 lsophorone Grab 625 5.00 ND ug/l 1 Naphthaiene Grab 625 5.00 ND ug/1 1 Nitrobenzene Grab 625 5.00 ND ugh 1 N-nitrosodi-n-propylamine Grab 625 5.00 ND ug/1 1 N-nitrosodimethylamine Grab 625 5.00 ND ug/l 1 N-mtrosodiphcnylamine Grab 625 5.00 ND ug/l l Phenanthrcnc Grab 625 5.00 ND ug/1 1 Pvrene Grab 625 5.00 ND ug/1 1 1,2,4,-tricniorobenzene Grab b2b b.f./0 NU ugh 1 I certify under penalty of law that this document and all attachments were prepared under my direction and supervision in accordance with a system to design to assure that qualified perdonnel properly gather and evaluat the information submitted. Based on my inquiry of the person or persons that manage the system, or those persons directly responsibel for gathering the information, the information submitted is , to the best of my knowledge and belief, true, accurate and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fines and imprisonment for knowing violations. Form - DMR- PPA-1 tam wooa Authorized Representative name Signature 1/ /1U Date Page 3 [nvironmontalTesting Solutions Inc. PO Box 7565 Ache it lc. NC 28802 I'hanc: (8281350-9361 nix: (8281350-9368 L-maii:.iinrei'etsnclab corn 1)4aTc Au ens,. 20.2018 EI'lltrent Aquatic Tozicits Rtport Form - Chronic Fathead Minnow Multi -Concentration Test racilitg: Andrews WWTP NPDES NC- 0020800 Pipes • 001 Count%: Cherokee I .ulxtratan Pet lest Environmental ['or ft )' clutu n;5.. Ijnc s Ignature operator in Responsible Charge ,tcP „/"� � �` _ir)Q/ • immune of I.ab rattxt Super%icor / ^ ( ommcnts :'tote.-7- I:991 Samples' 10680(701. I A )8Cifi ? . 11.11) 10.:5 • Mail Original To: North t.'arclma Deparanent of Environment and Natui ti Rootlet::: DWQ, f?m it mmcrrtal Sciences Branch 162 i Mail Sin ice (.shier Rsletph. NC 27699-1621 Control Orpanisnis % Effluent Effluent Replicate number 1 2 3 4 SumvinsE rumher of laresc 10 10 10 10 Ullcmal cnmt er of la • a: 10 I 0 10 10 Weleht 0ri:ritnl111ta larvae) 0.691 0.650 0.620 0.626 St'Ti4i:tp numhe: oflzr ae. 10 10 to 10 ulr,•atal number 10 10 10 10 uaahronfooaleoi =star) 0.666 0.663 0.653 0748 5I44\6iu_ m_rher of I:Ir.:4. 10 10 10 10 _ _ ra` •• :u- per of Inr.: 10 70 10 ICI 0 706 0 770 0.542 0.666 I7f(l aerll •.u.... ..• r .'(::.. .s•.a I5. :a.-.ar 111 111 10 10 0.6_ 8. IC 0.630 10 0 683 In 0.677 % Effluent - I .. • - r.::,tr: of Ixsoe 10 10 In 26 "'a Effluent IEEE I uul n.ur.:er er Ian ae 10 ld 10 U e i:ht ongineI 1 wig 1aj, ne) 0.670 0695 0 • t'.775 5a: t i , i;:4 number of Ian w 10 10 10 10 Origilctl turn]:1 •,11a,ae 10 10 10 10 acl rl,,,''.r-1 m iris 0.703 0690 0.701 0.646 V. ate, Qunli Data Control } •L:Li ,▪ 74 ICDuren - t...:9 t 1. Steal (lair: Fnd 447 Stet tune OS. I4.IS 01159 S.u: na7 .°°, '•tern°r wr Itng� A. cap; w: st'.I%it ttIC'rr: Sun i, t •. e. a• c t.; I,r:E1 Sur, a. I •-r Sirn-itnl 1't1 1t errMc ur (Ing 4unnal 1,0 \t KTr,�T ff:lgI 100.0 0.647 t1.617 100.0 0_683 100.(I 0.686 100.0 6.65- 100.0 0.720 100.0 0.(185 Teat Organisms S Ii&e:C 117,1 , x Coln :re trcc:n I:m;h rad 1.4 cil 1S.06.IS 171I. Ox-07-:S r : r list o real' Dal I Irli:a r %vial DRY retrial 1 $l Da), 3 Lllual Erna! Dar 4 Initial I Final Day 5 banal Das 6 t:,nu a real -I 7 50 768 7.43 1.61 7 -. -- 4:• 7 71 --' f . -• -.S -t;' 'i 't` ` - - .i -•S ,--' .i7 : - - - 4-1 _ sl . x» -.a' 7 28 _I - _. s SI 5G Si 68 5: -I Ft c-, 24.9 21 1 ?4 S 24 0 15 C 24.7 _` n _ _ • _4 r 24.9 _- tiample Inforutation C,.., dal: stardare Citall Cpi: x:ae dlrrt• -.• iliainin un,T Ilardt•e$I Tn:nl _•lr::nr_ SamTlr Ter10 a: Fte:al.: ; • S2::1Ph 1 Sample 2 Stvnplr 3 L. _ c ,l 08-0i-18 05.09-Ir tell Overall Analysis: Result. PASS I-OLt_. NO[(. ;:11..; i'il`'. 5_^nai 'leas}: �te5 1 % 1 wisi Imp ,Ir vieal 1::... ;: ' tfrita.o; ( rural Cakulatcl -cal Ca..utnred -'- ° - -• .-0 • • D IS'() fair, .4 7'- 5 0.'03) P() Box 7565 Asheville. NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. August 20, 2018 Ms. Wanda i.add Earth Environmental Services 75 Bison Lane Murphy. NC 28906 RE: ETS PROJECT NUMBER: 13491 Dear Ms. Wanda Ladd: Lnclosed are toxicity test results for samples from the Andrews WWTP received by Ent ironnlcatal Testing Solutions. Inc. August 06 through August 10. 2018. Parameter Test Procedure Code EPA Method Num her Final Result TliP(iC' Fathead Minnow (Pirnephales promelcts) Larval EPA-821-R-02-01; C'h\ >52% Survival and Growth, Test Method 1000.0 (.:hv = Chronic Value. A numeric % iluc rcprc entire the geometric rncart of the highest concentration having nu d, ,,:table impairment of survival or growth and the lowest roncentrtrtion that does have a detectable impairment of survival or nr,ntitlr. If this test was performed as an NPUES requirement or by Administrative Letter, please enter the Chit value >52% on the Effluent Discharge Monitoring Form (MR-1) for the collection date August 06, 2018 using the parameter code TIIP6C. Additionally, please sign and submit the original i)WQ Aquatic Tu\ieitr Form (AT-5) by September30, 2018. If you have any questions concerning these results. please feel free to contact me. Sincerely. Jiin Sumner Laboratory Director fhis report should not be reproduced. except in its ctttiret. ciithout the urine') consent oflimironmental Testing Solutions. Inc. 'Ihe results in this report relate only.to the samples submitted for anal sis. North Carolina Certificate :t'rtntherc_ Biological Analyses: 37, Drinking Water.. 37786, Wastewater: 600 South f'aroiirra Certificate Manner: Clean Water Act: 99053-001 PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) i50-9368 Environmental Testing Solutions, Inc. October 22. 2018 Ms. Wanda I, add Earth Environmental Services 75 Bison Lane Murphy. NC 28906 RE: ETS PROJECT NUMBER: 13632 Dear Ms. Wanda Ladd: Enclosed are toxicity test results fir samples from the Andress WWTP received by Environmental Testing Solutions. Inc. October 08 through October 12. 2018. Parameter Test Procedure Code EPA Method N umber Final Result 1.�,6C, Fathead Minnow (1'unepJiale. pronrelas) l.arval EI'A-821-I2-02-0 I ChV >52"/0 Survival and Growth. Test Method 1000.0 .Vote: ChV = Chronic Vnlue. A numeric value representing the geometric mean of the highest concentration ha%ing no detectable impair writ or sun ital or growth and the lowest concentration that dues ha%c a detectable impairment of survival or growth. th. If this test was perlormed as an NPDES requirement or by Administrative Letter. please enter the ChV value >52% on the L•'fllucnt Discharge Monitoring Form (MR -I) for the collection date October 08, 2018 using the parameter code TI IP6C. Additionally. please sign and submit the original 1)\VQ Aquatic Toxicity Form (A l -5) by November 30, 2018. If you have any questions concerning these results, please feel free to contact me. Sincerely, r rWl6M�—� Jim Sumner Laboratory Director this report should not be reproduced. c..:ept in its cntirct'. without the written consent of l..m ironntenlat I rating Solutions. Inc. foie results in this repm relate to the s unplrs submitted for annul sis. North Carolina Certificate .Vurnher•s: Biological Analyses: 37. Drinking Water: 37786. Wastewater: 600 South Carolina Cent] icon! Vunher: Clean Water Act: 99053-001 ' Environmental Testing Solutions. Inc. PO Ito, 7565 Asiwillc. \i 288(12 Phc?nc: (828) 350 9361 Fay 18281350-9368 1:-mail: Jim i±etsnclah.cnnl Dale' (tctob2r 22.2018 Effluent Aquatic Toxicity Report Form - Chronic Fathead Minnow Multi -Concentration Test Fai ili1) %ndresis 1'11�TP NC 0020800 Nips is 001 Counts: f'hrrokcr Laboratory Performing Test. Environmental Testin} Su lion. Signature of Operator in Responsible Charge: Signature of Laboratory Supervisor C(Intmenls• Proj&t. 13622 Sample 18010a 01, 181010.30.18101222 flail Original To: North Carolina Department of I-ns-ironmcnt and Natural Resources I)10x Environmental Scicnocs Branch I62I Mail Service (-enter Raleigh. N(' 27699-1621 Control Organisms "/a F.mucnt OMB Replicate number 1 Seeming number of lame 10 10 1 ft 10 otigirsl masher Alarm 10 10 10 10 11 enrhr nnlmml (rngjler.aeY (1.700 0.799 0.894 0.926 % Effluent 6.51f. % Effluent I 1 % Effluent EMI °6 Effluent Su1unag mmitlxrof(nrrn: t0 ill 10 10 (}.lnnnal number of fan tw 10 10 10 10 Wc10111,0npi1tnl (16S, 111nx1 (1.89(i 0.899 1.016 0.942 -, . I'. :.euit117 of larvae 10 -- 10 10 10 Ca.;'nrtl final,/ orlanae 10 10 Ir1 10 A'eiiph(v3irnrml lmgianart 0 9_ 7 0.916 0.833 0.833 Sw.'rvins, nnm1R-r of lsa. 1,1 I O 10 10 t)n_1ir-'1 mnnkm,rttat,a. 10 1:' I !o Pe ,�¢ram.'x, u 3 0.i,2' '1 -41 0890 Nemfnng nwnb.t of !au ar 10 10 10 111 Onitinalnum4.-1oftatcae IQ 10 ID 1(.1 Heigh* inilmwl tap larcnel 0.863 0.892 0.933 0.916 Slrvtvtni wake! of Ianae 10 10 III 10 Orir:itul mother et 0f lannr 10 111 10 10 \tcivil' ocletrul(mplena:l 0.829 0S79 0.834 0.884 \' liter Qualiy Data Control if(ISU 1. Uu tuts 11: Tpnp I C' IIigl1 (:lln(cn Start &me IU-UY-I5( Stln ivtt r'•. I lc'cra,t wtlnit: I cra:Y 1,1; Astaire WI Ir1 • StA,Ival l'.. : vest'[ w7 In,•: S11niv h",1 111 Crar.0 N{ I111i 1 1-rd rune' 10tt..IR 1(10.0 0.846 0.846 100.0 0.938 100.0 0.880 11111.11 0.855 111(I.0 0.901 100.0 0.8S7 Stan tune: OS37 fest Organisms S(ru�1: End hme I 0714 In-house Cullarc I fiesta; hatch I.rld hat-.11 111-O8-18 I625 10-09-18 U60U pity 0 (final 1 Final Day I tar •1 1-•um1 Dab 2 hnrwl I { rind Dar 3 Intnal I Final t):IV 4 1rR:1.1 I16n1 Dm5 lni ai final Dap 6 Irma! I Final 751 - 71R ; • i.74 7i3 4R9 7.3: 26 '1+ (i97 727 6Ri -;' C^ h - 7S -i- 8.0 'C : .4 24 '. 21 i -. _ I .1 ., _ '1 : '4 - 2; t _ I t• :'1 -. 711 t.9: .I - ,., - 'I ! .,, -1' - > . 7.1' 77 'R Si -' . s.1 -. -- ._ '11' '15 240 24.4 2I9 2-2 '4S .. 21X 'J: 24:' J r, Sample Information (.dlect, m start dal,. Grit• Cuuip,',]t. duraaon .•Ul5luun 'mu 1 C•nl'1),. I Ixtlnr.•.1 I - C 3' I . '1! .,.1.I.Kt.:LI. S:cui....::q• :: nece•r•; C Sample 1 IIMM Santi ('0111In1 1011748 10-09.1% 10 list [ 1 21 25-b . :-It 24 "2-h I-----1 16 ..• ii 10-:0 21 4 $I ?6-Ill 2V7 'su IIII 1i' •:010 11 1II) - 14 r. ()serail Analysis: Result: PASS LOEC: >52% NOEC: 52% C'hV': -ltial).e. mina& H,_ m. \(s(-(- • J'1 St:.c"cai Cirnwth S cn yes 'Cc* 'Sea. 72". . .ill. ..2"t .chi. `.'1..1.d Insr, Ihamctt'., • .. _I ...:.::11 (akhlarei ,trod) Cpjii 1 a Calculated . _: 21IU •2_601 ' - :ail 'Iy4'1 >52 u/n Dff Q form .-1T-5 (8473) PO 13ox 7565 Asheville, NC 28802 Phone: (828) 350-936,1 Fax: (828) 350-9368 EnvironmentalTesting Solutions, Inc. November 30. 2018 Ms. Wanda Ladd Earth Environmental Services 75 Bison Lane Murphy. NC 28906 RE: ETS PROJECT NUMBER: 13728 Dear Ms. Wanda Ladd: Enclosed are toxicity test results fir samples from the Andrews WWTP recck cd by Environmental Testing Solutions. Inc. November 05 through November 09. 2018. Parameter Test Procedure Code EPA Method Number Final Result THP6C fathead Minnow (Pinrep/rales promelac) Laval EPA-821-R-02-013 ChV >52% Survival and Growth, Test Method 1000.0 1 nIe: (:hV` = Chronic Value. A numeric Falur representing the geometric mean of the highest concentration lun ing no detectable intpairrnent of sun h al or growth and the lowest concentration that does have a detectable impairment of sun ival or growth. If this test was performed as an NPDES requirement or by Administrative letter, please enter the CIN value >52% on the Effluent Discharge Monitoring Form (MR -I) for the collection date November 05, 2018 using the parameter code THP6C. Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT-5) by December 31, 2018. If you have any questions concerning these results, please fccl free to contact me. Sincerer. 4.441441404`.— t -I Sumner Laboratory Director this report should not be reproduced. except in its entirct,. without the written certsrttt of umvironntental resting Solutions. hie. I he results in this report relate old:, to the samples submitted tor anal%;i.. Yorth Carolina Certificate Azumbers: Biological Analyses: 37, Drinking Water: 37786. tl'astewatcr: 600 South Carolina ('errilkale Vt,nrher Clean Water Act: 99053-001 • •• .3 Environmental Testing Solutions, Inc. PO Box 7565 Atic\'i11e.NC28802 Phonc: (828) 350-9364 Fax: (828) 35U--9368 -mail; Jim ii;ctsnclab.com Date. Not ember 3u, 211I8 Effluent Aquatic Toxicity Report Form - Chronic Fathead Minnow Multi -Concentration Test Fmclh;\ \ndrens 11'111TP NPDFS 0020800 Pipe #: 00I County: Cherokee I aborator Pcltonning Test. 1.mimnmcntal Tcstiu luhl Sitglatute of Operator in Responsible Charge. Signature of I ahnrat4n Supnkix,r. ('ommcnts: Ikupc�r 13728 Samples- 181 105 05. 1811 ]Sail Original To: North Caolina I)epllnmcnt of Ur%honntcut and Mutual Remiurcc; DWQ. I-.n.. ironmental Sciences Branch I621 Mail Sets Center !taleieh. NC 27699-16?1 Control l hrganisms % Effluent Kftlnertt EMI % Effluent EMI % Effluent IF= a Effluent Replicate number I 2 3 4 Srr.+n inp numl) 1 of far, a. 1(1 10 1(1 10 Diii:inal amnia./ of Isvae 10 10 10 10 1\citht•onglnatlme.'brear) 0.675 0.708 11,7411 0 (173 Slrrci+ILIA, itunJbl.t of l_i.ae 10 10 10 11) Ucuind manlier of lame 10 10 10 ill 1\ esghr •mptnal r ntp1ana;.) 0.665 0.692 I) (fi3 0 613 S n i, ug nunlur or Can nr 10 10 10 III i h initial samit.a7 :.ar,.:3 III. 10 ! 0 III 11aaht'Wua:n.r:imi: ar'..::I 0655 0.694 0-7f16 11.7011 Su..r :n•. n:nn r, of Ii, •.,- II 1(1 10 111 (lnrtnnl mmth;t of la,J: I I 110 ID 1(1 Wrinh:ptiyeittaluul lat+ael I:t,'.: i•13 '+.648 _ 0.704 Sus%, ale menherofIan ar 10 10 10 1(1 ()n_ttnal lumber of lawn. . 10 1(1 I 0 10 A'elpin original (nu.l.c.ax1 0.627 0.660 0.653 0.690 S44,uutc auutb.i of lanae 10 10 10 _ 10 Otigirul number of lama: 10 10 111 10 Weightoriginal fine Iat,ee, 0.758 f) 717 0.726 0.787 11 titter Qualify Data Control n11011 110in;•II lamp r High Conceit pi! is.' I' r,n ow. Li. Temp I C, Stun dst; tfal U. Start time' rod lime I I d.,6-11 11.13-11t OS 7 08-10 Sins nal(".n t+entre'm 111121 A, faze Mt - xi. n ng imgi A, eta¢. ,,. Stu I :‘,,I... Su, xcage ni only) Su, n al 1' .1 :\Coale (I tnla•1 1011.0 0.699 0.699 100.0 0.6511 100.0 0.689 11111.1) 11.66'1 1011.11 0.658 100.(I 0.753 Test Ori ani.nis S,+tacc: 16.811 tse ('tilnre , $:gits loath_ I:ud kW, II-05-1S 1700 114)&18 0515 Mv0 h.ittal Maul Day 1 Imnnt I Final My Initial I final eta. 3 Irattal1 final (meal I Final My luau! ) term Da. 6 In.bnl I litt:.l 7 06 7.1)2 7.1.1 6 97 - . - 1. - 1 f, ':S - . 7.22 6 9: '.17 6 96 `' 79 't '' -. 3;! 21' '2.4 249 ;L,. .. -4- 2.I'. - .. 'JR 73.6 21' :IS F1 'S 8.1 7.7 8.2 -. -- -, -. 8(I H , . 7.9 250 74: >>r 245 249 '41 ,_S 21.7 -.. 7,46 _ . 2I.1 246 Sample Informal' l .:..; 15,a) t .rnp'nulu .1lta dims, n pl (..3 Ilarch.y:Ict,1 Cal. ' t ,Kaitutnm nunh„unrr 'rat residual .IJurr,z iu:x Smnpte Temp ar Re:r,r• • _::.pfc I Sample 7 Sample " r -an? 1-1 -1,. 11 •rot• 1: I 1-08-IS i. 58 61 :r..a 267 2G7 -.. -'(111) 'I10 ,.0 :C. I3 1.1 I' Overall Anal, sis: Result: PASS 1°F.C: >52% NOE(': 52% CIA': nal}Sc. u•:i,c.1 Gr.».tn Y,-t ,I bop. Duraait's ,.It.',.I Grovib • --I:I.-. i -r-: ,,., . 1..al ...-.:laled ._ - I 19 ' r i l l _ 1 YJ ., "IS AD'Q fom,.l7=i (X 17.71 Pt) Box 7565 Asheville. NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. August 20, 2018 Ms. Wanda Ladd Earth Environmental Services 75 Bison Lane Murphy. NC 28906 RE: ETS PROJECT NUMBER: 13491 Dear Ms. Wanda l.add: Enclosed are toxicity test results for samples from the Andrews WWTP received by Environmental Testing Solutions. Inc. August 06 through August 10. 2018. Parameter Test Procedure Code EPA Method Number Final Result TI1P6(: Fathead Minnow (Pimephales pry» nelas) I.arval l; PA 82I It-02-013 Chi' >52`% Survival and Growth. rest Method 1000.0 Note• CM' = Chronic Value. A numeric value repr. scnting the geometric mean oldie highest concentration having rx) detectable impairment of survival or growth and the lowest concentration that does hay e a detectable impairment of survival or grov. il'this test was performed as an NPDES requirement or by Administrative Letter. please enter the ChV value >52% on the Lfuent Discharge Monitoring Form (MR -I) for the collection date August 06, 2018 using the parameter code THP6C. Additionally. please sign and submit the original DWQ Aquatic Toxicity Form (AT-51 h) September 30, 2018. Ii you have any questions concerning these results, please feel free to contact me. Sincerely. Jiro Sumner Laboratory Director This rep on should nut he reproduced. except in its cntirct. w ithout the'. riucn consent of Ern i/mum- tal I esting Solutions. Inc. The results in this report relate only to the samples submitted for aual� sic. :North Carolina Certificate Numbers: Biological Analyses: 37. i)rinking Water; 37786. Wastewater: 600 South Carolina Certificate A'umuber: Clean Water Act: 9905 3-001 E nvtronmental Testing Solutions. Inc Effluent Aquatic '1 oxicit} Report Form - Chronic Fathead Minnow PO F3nx. 7.56i i1sil. t it Ir. NC 2S80 Phone: (828) 350-9361 I-rtv (8281 : j11-9368 rr Inaii: Jim•a etsnclab cam I)3ra .iueuit2u _i IS \ 1 ulti-Concentration Test ra;ilitt. Audi -cm ' vrp NPDES = `.0 I►U20800 Pipc y 0h1 Sign turn Pct fOpforming 1rt 1 nsiEnsble Chartal Cc,t:ne;ti; iuu a Ala/ Sir afore nC Operator an Responsible Charrc•1 Sienaturc cf Lab.iratun Supm ianr C.l,mmcros- Protn-t 11,4 )1 Samples* I8ISL* OI. IFOROR ).. I IID810.=U Mail Orizinal To: North Carolina Department ut i-.^ti in:mino a1 , `,n:w tl Re,otircr. I)l1'Q ratiionnterns! Scl nccs Brandt 1631 Mail Srr. . ice ("cnrcr Raleigh \(" 270:•99 1621 C'ontrnl Organisms % Effluent 3.23% 4•o Effluent Fftinent l is: 1 % Effluent EMI "b Effluent 51.,. Replicate number t 3 3 i iu:, nita numb= of lar.ac 100 10 10 10 fhtcmal nonslx or let, a: 10 10 10 10 tlnph:0ririrulutt`Fetid 0.691 1.'.650 0.620 0.626 Sim i.ino number ofisnoe 10 i0 10 1(1 CR:•anai :alu lba of inns. 10 10 10 10 tt.acnt errioiryl r,r=,:la.ac) 0 (00 0 663 0.653 0.748 _ number oflanne 10 :i.. (f) 10 - _ r.,. -amber p`Far.a: 10 1(, 10 • . ...' nn::Itr.a.<1 0 706 0 779 f}_n.l' 0.66%. al., .... f::..: ::,ax III jf I1 ICI ... -: I :-.ae 10 Ii 1.1 Iri ,. --.art 061S 0030 0683 il.6-- munbcrof!fully 1:41,Tizoi rllatnber ;!flame ttbrcla'anginaf S no: If a1:S 10 10 0.670 10 IC 06455 10 (I ':4 I f: S.i.i,n:u:arntre etlam ne 10 10 iI '1 41:-;11,a1nwn cr.•:lance 10 I(1 11 ‘.tacit m!4rrl1.11To. lrr:,a,I 0.70.4 11 (iW., r.1. -. « ater Dale Control -iI `I'1 Iligh Concentration • yJ rzr_ 1.r Stet date OS-07-IR Frei dare OF F4-IS Sian tune re•'. r� (Ixs.J :I,r "I hnni 100.0 0.647 0.617 100.0 0.683 100.0 100.0 0.657 100.0 0.720 100.0 0.685 Teat Organisms C.yutt b-hnre,e '. •11• "c tSec:- Its;4 rn3r:axL f.S _IG 1-'II OS.; -. Wyo Dad 1 Da! Da. 3 Day 4 Day; lnm;li Final bu::a r Ir.- al I F • :1 I:nuxi renal in r i•i1.11 Inc: Final tninal 1"-,_:1 Dash .'a :? :G,p - I1 -c: 4] 77i -'c ':R ' 7 _y _.L - S- -- .5 __ 7 , ..� .•. x1 - _ -� - I - '.4: -�; -_� '- - • o bO -- sl -. 71 56' 6v S, _=.'• =1 I ?SA 246 . . - - . - - V `4' -4!1 2.: 7 7.5.•;. Semple Information ...ar . r:1.1 I-' -I• Uv era ll Analysis: :.rtpie 1 Sample 2 S. 6a•li3.13 os.07-IS <l •" 24 2l-FI SE :R Analyses .1 n'ar • . '..:talrlp. rh.nr_KF Su. -.:gal Gt:rn:: . EEwen! C'rtn.-13 Cal<ular"1 • 7 ,:.,I Ce.:1 ._ . . eia 17.% • - -0 : . 2rj''.. • ReSL.l: PASS I.OEC. '52% NOEC "n i II\' :.52% DUD foruf.-11' (8'.0j PO Box 7565 Asheville, NC 28802 Phone: (828) 350-9364 Fax: (828) 350-9368 Environmental Testing Solutions, Inc. August 20, 2018 Ms. Wanda Ladd Earth Environmental Services 75 Bison Lane Murphy, NC 28906 RE: ETS PROJECT NUMBER: 13491 Dear Ms. Wanda I.add: Lnclosed arc toxicity test results for samples from the Andrews WWTP rci ci' cd b Lin ironntental Testing Solutions. Inc. August 06 through August 10. 2018. Parameter Test Procedure Code EPA Method Number Final Result Ti1<P6C Fathead Minnow (Pinrephates promela5) Larval CPA-821-R-02-013 ChV >52% Survival and Growth. Test Method 1000.0 Vote- ChV = Chronic Value. A numeric value rcprctenting the geometric rnean of the highest concentration having no detectable impairment of sun i%al or growth and the lowest concentration that does have a detectable impairment of survival or grcoth. If this test was performed as an NPl)ES requirement or by Administrative l .etter, please enter the ChV value >52%, on the F.liluent Discharge Monitoring Form (MR-1) for the collection date August 06, 2018 using the parameter code THP6C. :'Additionally, please sign and submit the original l)WQ Aquatic Toxicity Form (AT-5) by September 30, 2018. If you have an) questions concerning these results, please feel free to contact me. Sincerely. Jinn Sumner Laboratory Director [his report should not be reproduced, . cept in its entirety. k%iUwut the %%ritten consent of Environmental Testing_ Solutions. Inc. I he result: in this report relate only to the samples .submitted for analysis. •Vorrh Carolina Certificate Numbers: Biological Analyses: 37, Drinking Water: 37' 6. Wastewater: 600 South Carolina ('t'rtif leare Vionht'r: Clean Water Act: 9905 3-t:u)1 PO Box 7565 Asheville, NC 28802 Phone: (828) 350-936 I Fax: (828) 350-9368 Environmental Testing Solutions, Inc. August 20, 2018 Ms. Wanda Ladd Earth Environmental Services 75 Rison Lane Murphy. NC 28906 RE: F.TS PRO.JF('T NUMBER: 13491 Dear Ms. Wanda Ladd: Enclosed are toxicity test results for"samples from the Andrews WWTP received by Enx ironmental testing Solutions. Inc. August 06 through August 10. 2018. Parameter Test Procedure Code El'A Method Number Final Result THP6C Fathead Minnow IPim/Tlurlrs promelas) Larva) FPA-82 I-R-02-013 ChV >52%, Survival and Growth. lest Method 1000.0 Vote: CtiV = Chronic Value. A numeric value reprinting the geometric mean of the highest concentration having no detectable impairment of sun i'i al or gru\\ th and the l00%est concentration that does have a detectable impairment of survil al or er n tli, If this test was performed as an NPI)hS requirement or by Administrative Letter, please enter the ChV value >52°A, on the F.Illuent Discharge Monitoring Form (MR-1) For the collection date August 06, 2018 using the parameter code THP6C. Additionally, please sign and submit the original DWQ Aquatic Toxicity Form (AT-5) by September 30, 2018. lt' you have any questions concerning these results. please feel free to contact me. Sincerely. Jim Sumner Laboratory Director This report should nut he reproduced. except in it• rrrriieq. vsitho tt the %%ritten consent of Environmental Jesting Solutions. Inc. the results in this rclxan relate onl} to the samples submitted for analysis. North Carolina Certificate Numbers: Biological Analyses: 37. Drinking Water: 37786, Wastewater: 600 Sande ('ca'uline: ('era ic:fi .A umber: Clean Water Act: 99053-001 Environmental lusting Solutions. Inc. PO Box'565 Ashen ille. \C 28802 Phony 8281 350.9364 Fax. l$211) 350-9368 F-mail: Jim•n etsnc[ab.c m Date. August '6. 2111$ Effluent Aquatic Toxicity Report Form - Chronic Fathead Minnow Multi -Concentration Test acrh:s Andress %t'tv'1'P NPDES NC- 0020800 Pipe + . 001 C buns • Cherokee Laburatvr', Performing T(,t. Environmental Tcstina Soluilirtl, Inc: Signaulrc ci Operator in Responsihlc Charge j t�t� Si_nanue n1.1 stxiatorr Supurs Nor Comment' Ittlxa 11491 Sam;$ 18f18',t. J!. 181.l8016.23 1S0S10'6 mail Original I n: North Carolina Department co 'rrs 1rnnntt n+ +r+J s:at Dal Rea urco LWCl. kncironmc•nial Sctcnccs 0ratich 16? I Mal Sett icc (•cnter R:rleiph. NC ?7699. 1621 Control Organisms % Effluent Lllluent 16.5':: Effluent l'ffluenl EMI ° I.tllucnt Replicate number 1 2 3 4 5ur%uil r fium1,0 of tame 10 10 10 10 Chiginal number of lama. 10 10 10 Ill welpItt ptigi nal Fang lame; 0.691 0.650 D 620 0.626 Sunning ntun1'-r ul Iryae 10 10 10 10 Ongiral , b i of larvae 10 10 10 l0 steiehr:nri;, ar47anx1 0.666 (1.663 0 653 0 74S Scti, arc: n.arhtr.af Iann: 10 10 II) 10 hnr:rrt ruma;t v1Ianae 10 10 I U,� (r.6;;6 Aen'-;,:I rl::arIanir. 0_•06 (i-24 0.6.12 1r.?. •:: r.::•0•.: e- Jr. 1:' 0 .:.•ra:::.crr:• f IC 1.) :II 1.:1., ..r .g.-, I ' _ 11 0.68.1 6.477 SW. It rn; nuir hz at Ilmae 10 10 10 10 Onsinal nwnio cr Ianac I O iC) 10 10 Weight ;,14inal frog. Ian ae, 0.670 n 695 0 739 0.7 T Statirrne nunber-ofla.. a: 10 10 10 i11 C;yri_ilral nr:•mbcr of lam;.- 10 10 10 r 10 sLtg:un;tn uaIrna.itlnacr (I703 0690 0.701 0.646 t\:tterQuali s Data Control i+,51. t lera.o I nay) I 1 i .. ...Inal I Furs tray 2 Intnal F nay 3 Start Cate' Frd t)at Slit time: 1_•, t18.07.18 n8 14-1S 0859 ral +'°) .l ensf`e w11m�7 1.r mpg WI ten Intl •:n : Sun ::craft %urilr� tier.1. • . S;rttti•. at i"rI 1: emir: ‚Sil n, u. Sta...41 1 `.'I 5'aa1c utItnu 100.0 n.61- 0.6.1- 100.0 0.683 100.0 0.686 100.0 11.657 100.0 0.720 I01i.0 0.685 nay 4 Test Organisms tree Imlloute ['Aire La:: I '13.07.15 Days r rIal 1 Final Irma; 7 r.a: Imta. " I-:' 3.0 79 a7 IIiab. Concentration _�: --- ".I I -.- ...1,-_ --- I: -.- -- t1 1 R : -- ... 5.3 SI `1 3"' 2:9 '4- _IS y., .. _:U _ 256 :.I� •. _i_ :r. ' 'Sotnplc Information :. Jtrc 5e;rp;c i S.tip!r 88-I::48 ns-6i-t8 ! "•-1, 59 81 • I 9r :t5i 10rrarg Overall Analysis; Rt_5,1lt' PASS r (EC. =>52",. NOLC. 52% ChV: :tnalvarc Item r Su .:.A °.L•tllrmru Cr:ricat Calwlatr:I Cn;::.-1 :.;t: •.;a I: r _ 5,2' WO 4T-; j$.'031