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HomeMy WebLinkAboutNC0001422_Fish Tissue Monitoring Study Results_20220329DUKE ENERGY® PROGRESS March 28, 2022 Certified Mail # 7019 2280 0001 4397 3327 (2 copies) NCDEQ-DWR Water Sciences Section 1621 Mail Service Center Raleigh, North Carolina 27699-1621 Subject: Sutton's Fish Tissue Monitoring Study Results Dear Sir or Madam: L. V. Sutton Energy Complex 801 Sutton Steam Plant Rd Wilmington, NC 28401 Enclosed is Duke Energy Progress LLC's (Duke Energy's) NPDES Fish Tissue Monitoring Study Results. This submittal is in accordance with condition A. (11.) of NPDES permit NC0001422 for the L.V. Sutton Energy Complex which became effective July 1, 2020. An electronic version will be emailed. 0 910.341.4750 t 910.341.4790 If there are any questions, please contact either: • Mr. R. Glenn Rivenbark, NPDES ORC for the L. V. Sutton Energy Complex; phone (910) 341-4774, e-mail Roland.Rivenbark@duke-energy.com; • Mr. Kent Tyndall, EHS Professional for the L. V. Sutton Energy Complex; phone (910) 341- 4775 or e-mail Kent.Tvndall@duke-energy.com; or • Ms. Elizabeth Glenn, Environmental Specialist in our Environmental Permitting & Compliance group, phone (980) 373-0530 or email Elizabeth.Glenn@duke-energy.com I certify, under penalty of law, that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fines and imprisonment for knowing violations. Sincerely Jame o • her General Manager C: via email Sergei Chernikov NCDEQ-DWR NPDES 1617 Mail Service Center Raleigh, NC 27699-1617 via email Morella Sanchez -King NCDEQ-DWR, WiRO 127 Cardinal Drive Extension Wilmington, NC 28405-3845 L. V. SUTTON ENERGY COMPLEX 2018-2021 FISH TISSUE MONITORING REPORT NPDES Permit # NC0001422 April 2022 Water Resources DUKE ENERGY PROGRESS, LCC. New Hill, North Carolina Table of Contents Page 1.0 Introduction 1 2.0 Study Site Description and Sampling Locations 1 3.0 Target Species 2 4.0 Field Sampling Methods 3 5.0 Laboratory Processing and Analysis 3 6.0 Data Analysis and Reporting 3 7.0 References 5 List of Tables Table Page 1 Sample location, species, number collected, and size (length and weight) of fish collected for trace element analysis from 2018 through 2021 4 2 Means and ranges of trace elements in fish muscle tissue collected from the four sampling areas from 2018 through 2021. All values are in wet weight (µg/g) including the USEPA screening values shown next to each element. Non -detects are represented by <DL (less than detection limit) in the table. 5 List of Figures Figure Page 1 Sampling areas for fish tissue trace elements monitoring on the Cape Fear River and Sutton Lake. 2 i 1.0 Introduction Duke Energy owns and operates the L. V. Sutton Energy Complex (Sutton) located adjacent to the Cape Fear River near Wilmington in New Hanover County, North Carolina. The Sutton National Pollutant Discharge Elimination System (NPDES) Permit (No. NC0001422 Section A.19) requires monitoring of trace elements (arsenic, selenium, and mercury) in fish tissues within Sutton Lake and the Cape Fear River annually (Figure 1). Fish were collected according to the study plan submitted to the NCDEQ- DWR and mentioned in the NDPES permit (dated October 1, 2017) and the resulting data are submitted in this report (Duke 2018). 2.0 Study Site Description and Sampling Locations Target species were collected from within Sutton Lake and the Cape Fear River. In Sutton Lake, the water is continuously or near continuously circulating, and is considered mixed and homogeneous, as a result, target species were collected from the whole lake as available. From the Cape Fear River, fish were collected in three areas (i.e., UP, DI, and DW; Figure 1). Area UP is located approximately between 1.1 and 2.8 miles upstream of the plant discharge and served as a reference location to monitor background trace element concentrations in fish muscle tissue. The area in the immediate vicinity of the plant discharge point (DI) served to monitor potential near - field uptake of elements in fish muscle tissue. Area DW, between approximately 2.1 and 2.6 miles downstream of the plant discharge point, served to monitor far -field uptake of elements in fish muscle tissue. 1 L V. Sutton Energy Complex 0 1.000 Z000 4,000 Feet I ill! Fish Tissue Monitoring Locations Cape Fear River (U P. DI. DW) FM Sutton Lake (Whole Lake) Figure 1. Sampling areas for fish tissue trace elements monitoring on the Cape Fear River and Sutton Lake. 3.0 Target Species The target species of fish for tissue analysis from both the lake and river were Micropterus spp. (i.e., Largemouth Bass) and Lepomis spp. (i.e., Bluegill, Hybrid Sunfish, Redbreast Sunfish, Redear Sunfish, or Warmouth). The study plan called for six individuals per species group to be collected annually within each sample location resulting in a total of 48 fish from the lake and 144 fish from the river. As recommended by the US Environmental Protection Agency (US EPA) for whole body 2 assessments, an attempt was made to limit the smallest fish to 75% of the largest fish total length by species depending on availability (US EPA 2000). 4.0 Field Sampling Methods Fish were collected using approved procedures (Procedure ESFP-SW-0600-Trace Elements Field Procedure -Rev. 0 applies to fish collected after 2020 and NR-0095-Trace Element Field Procedure - Rev. 1 applies to fish collected prior to 2020) under Duke Energy's North Carolina Biological Laboratory Certification # 006 located at New Hill, NC. Only live fish that showed little or no signs of deterioration were retained for analysis. Retained fish were identified to species, measured for total length (mm), weighed (g), individually tagged (Floy tags), bagged by species/site, and placed on ice and transferred to a freezer within 24 hours of collection following ESFP-SW-0600- Trace Elements Field Procedure -Rev. 0 (applies to fish collected after 2020) or NR-0095-Trace Elements Field Procedure -Rev. 1 (applies to fish collected prior to 2020). 5.0 Laboratory Processing and Arsenic, Selenium and Mercury Analysis All fish samples were processed in the New Hill Trace Element Laboratory according to procedure ESFP-SW-0601-Trace Elements Sample Preparation -Rev. 0 (applies to samples processed after 2020) or NR-00107-Trace Element Monitoring Laboratory Procedure -Rev. 4 (applies to samples processed prior to 2020). The processed samples (lyophilized left axial muscle; right muscle occasionally included when needed) were analyzed for arsenic, selenium, and mercury by x-ray spectrophotometry in accordance with procedure ESFP-SW-0602-Trace Elements Analysis Procedure -Rev. 0 (applies to samples processed after 2020) or NR-00107-Trace Element Monitoring Laboratory Procedure -Rev. 4 (applies to samples processed prior to 2020). Fish collected during 2018 were analyzed by Pace Analytical in the Green Bay, Wisconsin lab (As and Se were analyzed in accordance with EPA 6020 and Hg was analyzed in accordance with EPA 7374). Quality control was achieved by use of replicates and certified reference materials (i.e., NIST tissue standards). The remaining sample materials were archived and kept for at least two years in the event that re -analysis was needed. 6.0 Data Analysis and Reporting In total, 28 Micropterus spp. and 28 Lepomis spp. were collected and analyzed for trace elements within Sutton Lake, and 69 Micropterus spp. and 71 Lepomis spp. were collected and analyzed for 3 trace elements from the three locations on the Cape Fear River (Table 1). Fish species and sizes ranged with annual availability (Appendix A). Table 1. Sample location, species, number collected, and size (length and weight) of fish collected for trace element analysis from 2018 through 2021. Sample location Lake UP DI DW Species Micropterus spp. Lepomis spp. Micropterus spp. Lepomis spp. Micropterus spp. Lepomis spp. Micropterus spp. Lepomis spp. Number Length (mm) collected Mean Range 28 304 28 179 23 289 22 189 22 349 26 179 24 307 23 184 (242-379) (149-202) (200-349) (158-230) (211-451) (142-230) (245-391) (142-227) Weight (g) Mean Range 355 (157-665) 110 (66-162) 371 (97-583) 146 (80-222) 699 (134-1260) 124 (50-227) 447 (195-1020) 135 (52-233) At least six individuals per species group were collected from both the river sites and Sutton Lake, except for fish collected from the Cape Fear River during 2018. Flood waters from Hurricane Florence inundated the Cape Fear River with anoxic waters causing fish kills throughout the lower river (NCWRC 2018). Despite multiple attempts to collect trace element fish from the Cape Fear River during 2018, only a few fish were collected (i.e., one from UP and three from DW; Appendix A). Arsenic, selenium, and mercury concentrations (converted to µg/g wet weight) in the fish muscle tissue collected from 2018 to 2021 are shown in Table 2. 4 Table 2. Means and ranges of trace elements in fish muscle tissue collected from the four sampling areas from 2018 through 2021. All values are in wet weight (µg/g) including the USEPA screening values shown next to each element. Non -detects are represented by <DL (less than detection limit) in the table. Sample Location Lake UP DI DW Species Micropterus spp. Lepomis spp. Micropterus spp. Lepomis spp. Micropterus spp. Lepomis spp. Micropterus spp. Lepomis spp. Arsenic (1.2) Mean I Range2 0.16 0.16 0.21 0.17 0.30 0.20 0.27 0.24 (0.04-0.27) (0.03-0.25) (0.13-0.38) (0.13-0.22) (0.16-0.61) (0.11-0.35) (0.17-0.45) (0.16-0.41) Mercury (0.4) Mean' Range2 0.05 (<DL-0.20) 0.03 (<DL-0.04) 0.16 (0.07-0.31) 0.12 (<DL-0.32) 0.18 (0.08-0.33) 0.11 (<DL-0.23) 0.13 (<DL-0.31) 0.11 (<DL-0.34) Selenium (20) Mean' Range2 1.99 (1.59-2.70) 2.44 (1.76-3.39) 0.31 (0.19-0.39) 0.36 (0.20-0.63) 0.33 (0.20-0.53) 0.39 (0.17-1.32) 0.30 (0.23-0.41) 0.32 (0.20-0.67) 1 When concentrations were less than the detection limit, then half the detection limit value was used for statistical analysis. 2 Detection limits varied with year due to the use of multiple labs to process samples during the permit cycle. In addition to the length and weight of each fish, the dry -to -wet weight ratios are presented to convert the arsenic, mercury, and selenium concentrations wet weight values back to dry weight values as desired (Appendix A). The arsenic, mercury, and selenium concentrations in fish tissues were very low and showed no indication of bioaccumulation from legacy coal operations of Sutton. In addition, all fish collected from 2018 through 2021 were well below the US EPA wet weight Screening Values for Recreational Fishermen of 1.2 µg/g for arsenic, 20 t.g/g for selenium, and 0.4 dug/g for mercury (US EPA 2000; Table 2). 7.0 References Duke Energy. 2018. Fish Tissue Monitoring of Sutton Lake and the Cape Fear River. Study Plan. Sutton Energy Complex NPDES No. NC0001422. March 27, 2018. NCWRC. 2018. Wildlife Commission Biologist Investigate Widespread Fish Kills After Hurricane Florence. www.ncwildlife.org.News-Archives. October 4, 2018. U.S. EPA. 2000. Guidance for assessing chemical contaminant data for use in fish advisories. Vol. 1. Fish sampling and analysis. Third edition. EPA 823-B-00-007. United States Environmental Protection Agency, Office of Water, Washington, DC. 5 APPENDICES Appendix A. Arsenic, mercury, and selenium concentrations (wet weight) in individual fish axial muscle collected and analyzed from 2018 - 2021 for the Sutton NPDES permit # NC0001442. Species Arsenic Mercury Dry to Collection Length Weight Selenium Wet Species group Location Date (mm) (g) (As) (Hg) (Se) pg/L Weight pgil, Pe, Ratio Bluegill Lepomis spp. Lake 6/20/2018 177 102 0.04 0.01 2.02 0.21 Bluegill Lepomis spp. Lake 6/20/2018 167 88 0.06 0.01 2.18 0.2 Bluegill Lepomis spp. Lake 6/20/2018 149 69 0.05 0.01 2.38 0.18 Redbreast Sunfish Lepomis spp. Lake 7/16/2018 192 138 0.04 0.02 2.08 0.21 Bluegill Lepomis spp. Lake 7/16/2018 170 100 0.04 0.01 1.92 0.19 Bluegill Lepomis spp. Lake 7/16/2018 172 108 0.03 0.02 1.92 0.2 Largemouth Bass Micropterus spp. Lake 6/20/2018 273 213 0.05 0.03 2.18 0.21 Largemouth Bass Micropterus spp. Lake 6/20/2018 270 243 0.04 0.03 1.98 0.19 Largemouth Bass Micropterus spp. Lake 6/20/2018 311 346 0.06 0.06 1.98 0.2 Largemouth Bass Micropterus spp. Lake 6/20/2018 331 450 0.06 0.07 2.14 0.21 Largemouth Bass Micropterus spp. Lake 7/16/2018 341 480 0.09 0.04 1.78 0.2 Largemouth Bass Micropterus spp. Lake 7/16/2018 362 582 0.08 0.07 1.81 0.21 Bluegill Lepomis spp. Lake 3/21/2019 196 153 0.21 < 0.06 1.91 0.19 Redear Sunfish Lepomis spp. Lake 3/21/2019 189 111 0.21 < 0.06 2.17 0.21 Bluegill Lepomis spp. Lake 3/21/2019 184 99 0.14 < 0.05 2.11 0.16 Redear Sunfish Lepomis spp. Lake 3/20/2019 201 124 0.2 < 0.07 3.07 0.23 Bluegill Lepomis spp. Lake 3/20/2019 180 95 0.17 < 0.05 2.01 0.17 Redear Sunfish Lepomis spp. Lake 6/18/2019 195 128 0.2 < 0.06 2.31 0.2 Largemouth Bass Micropterus spp. Lake 3/20/2019 351 530 0.17 0.09 1.71 0.18 Largemouth Bass Micropterus spp. Lake 3/20/2019 335 467 0.17 0.07 1.65 0.19 Largemouth Bass Micropterus spp. Lake 3/20/2019 311 359 0.21 0.11 1.71 0.21 Largemouth Bass Micropterus spp. Lake 3/20/2019 326 398 0.2 0.1 1.67 0.21 Largemouth Bass Micropterus spp. Lake 3/20/2019 379 665 0.16 < 0.05 2.00 0.18 Largemouth Bass Micropterus spp. Lake 3/20/2019 363 596 0.18 0.2 2.32 0.2 Bluegill Lepomis spp. UP 8/29/2019 168 106 0.13 0.08 0.35 0.18 Largemouth Bass Micropterus spp. UP 10/8/2019 325 484 0.38 0.15 0.31 0.21 Largemouth Bass Micropterus spp. UP 10/8/2019 318 452 0.27 0.14 0.33 0.22 Largemouth Bass Micropterus spp. UP 10/8/2019 315 418 0.24 0.22 0.29 0.22 Largemouth Bass Micropterus spp. UP 10/8/2019 307 429 0.18 0.27 0.27 0.22 Largemouth Bass Micropterus spp. UP 10/8/2019 349 560 0.17 0.24 0.27 0.21 Largemouth Bass Micropterus spp. UP 10/8/2019 200 97 0.19 0.1 0.38 0.2 Warmouth Lepomis spp. UP 9/26/2019 191 168 0.16 0.18 0.34 0.2 Warmouth Lepomis spp. UP 9/26/2019 184 162 0.15 0.17 0.29 0.2 Bluegill Lepomis spp. UP 9/26/2019 189 145 0.19 0.12 0.34 0.2 Bluegill Lepomis spp. DI 8/29/2019 150 65 0.14 0.09 0.42 0.14 Bluegill Lepomis spp. DI 8/29/2019 163 73 0.19 < 0.06 0.42 0.2 Bluegill Lepomis spp. DI 8/29/2019 185 137 0.26 0.09 0.79 0.19 Bluegill Lepomis spp. DI 8/29/2019 168 91 0.18 0.15 0.44 0.19 Bluegill Lepomis spp. DI 8/29/2019 156 65 0.12 0.1 0.36 0.14 7 Species Species group Location Collection Length Weight Date (mm) (g) Arsenic Mercury (As) (Hg) µO- µ� Dry to Selenium Wet (Se) µg/L Weight Ratio Bluegill Lepomis spp. DI 8/29/2019 160 76 0.24 0.13 0.6 0.23 Redear Sunfish Lepomis spp. DI 8/29/2019 166 65 0.24 < 0.06 1.32 0.2 Redear Sunfish Lepomis spp. DI 8/29/2019 151 72 0.16 0.11 0.27 0.17 Bluegill Lepomis spp. UP 9/26/2019 184 133 0.15 0.11 0.47 0.19 Bluegill Lepomis spp. UP 9/26/2019 176 105 0.14 0.09 0.53 0.18 Redear Sunfish Lepomis spp. DW 8/29/2019 196 118 0.31 0.15 0.29 0.18 Bluegill Lepomis spp. DW 8/29/2019 166 90 0.2 0.1 0.67 0.19 Redear Sunfish Lepomis spp. DW 10/8/2019 158 65 0.35 0.08 0.41 0.21 Redear Sunfish Lepomis spp. DW 10/8/2019 142 52 0.41 < 0.06 0.44 0.21 Bluegill Lepomis spp. DW 10/8/2019 165 93 0.19 0.09 0.35 0.19 Bluegill Lepomis spp. DW 10/8/2019 173 105 0.38 0.20 0.49 0.18 Largemouth Bass Micropterus spp. DI 8/29/2019 309 412 0.18 0.30 0.44 0.19 Largemouth Bass Micropterus spp. DI 8/29/2019 211 134 0.17 0.24 0.3 0.19 Largemouth Bass Micropterus spp. DI 8/29/2019 305 462 0.19 0.24 0.35 0.2 Largemouth Bass Micropterus spp. DI 8/29/2019 330 584 0.16 0.33 0.25 0.23 Largemouth Bass Micropterus spp. DI 8/29/2019 296 380 0.25 0.12 0.36 0.21 Largemouth Bass Micropterus spp. DI 8/29/2019 325 520 0.21 0.25 0.53 0.21 Warmouth Lepomis spp. DI 8/29/2019 162 115 0.18 0.18 0.24 0.2 Bluegill Lepomis spp. DI 8/29/2019 161 82 0.16 0.18 0.36 0.2 Largemouth Bass Micropterus spp. DW 8/29/2019 283 340 0.45 0.13 0.41 0.23 Largemouth Bass Micropterus spp. DW 8/29/2019 294 370 0.29 0.11 0.36 0.22 Largemouth Bass Micropterus spp. DW 8/29/2019 288 356 0.31 0.09 0.35 0.21 Largemouth Bass Micropterus spp. DW 10/8/2019 303 418 0.31 0.18 0.36 0.22 Largemouth Bass Micropterus spp. DW 10/8/2019 340 582 0.28 0.14 0.34 0.21 Largemouth Bass Micropterus spp. DW 10/8/2019 345 598 0.29 0.15 0.31 0.2 Redbreast Sunfish Lepomis spp. Lake 3/19/2020 199 130 0.25 < 0.06 1.76 0.2 Sunfish (Hybrid) Lepomis spp. Lake 9/15/2020 202 162 0.25 < 0.06 2.34 0.19 Bluegill Lepomis spp. Lake 6/24/2020 177 110 0.16 < 0.06 3.39 0.2 Redear Sunfish Lepomis spp. Lake 9/16/2020 155 66 0.19 < 0.06 3.02 0.21 Redear Sunfish Lepomis spp. Lake 9/16/2020 173 101 0.19 < 0.06 2.92 0.2 Sunfish (Hybrid) Lepomis spp. Lake 9/16/2020 196 133 0.23 < 0.06 2.95 0.21 Largemouth Bass Micropterus spp. Lake 3/19/2020 294 299 0.18 < 0.06 2.01 0.21 Largemouth Bass Micropterus spp. Lake 3/19/2020 287 282 0.19 < 0.07 2.04 0.22 Largemouth Bass Micropterus spp. Lake 3/19/2020 282 250 0.2 < 0.06 1.79 0.21 Largemouth Bass Micropterus spp. Lake 3/19/2020 286 279 0.18 0.07 2.27 0.21 Largemouth Bass Micropterus spp. Lake 3/19/2020 298 304 0.18 < 0.06 1.59 0.2 Largemouth Bass Micropterus spp. Lake 3/19/2020 298 308 0.19 < 0.06 1.91 0.21 Redear Sunfish Lepomis spp. UP 9/22/2020 179 102 0.22 0.08 0.42 0.2 Bluegill Lepomis spp. UP 9/22/2020 158 80 0.14 0.11 0.27 0.19 Bluegill Lepomis spp. UP 9/22/2020 172 90 0.17 0.11 0.33 0.2 Bluegill Lepomis spp. UP 9/22/2020 212 208 0.17 0.11 0.43 0.19 Redear Sunfish Lepomis spp. UP 9/22/2020 188 124 0.22 0.07 0.44 0.21 Warmouth Lepomis spp. UP 9/17/2020 190 167 0.14 0.21 0.29 0.19 8 Species Species group Location Collection Length Weight Date (mm) (g) Arsenic Mercury (As) (Hg) µga- AWL Dry to Selenium Wet (Se) µg/L Weight Ratio Largemouth Bass Micropterus spp. UP 9/22/2020 342 583 0.18 0.16 0.27 0.21 Largemouth Bass Micropterus spp. UP 9/22/2020 330 462 0.23 0.16 0.33 0.21 Largemouth Bass Micropterus spp. UP 9/22/2020 325 547 0.23 0.09 0.34 0.21 Largemouth Bass Micropterus spp. UP 9/22/2020 336 578 0.22 0.1 0.36 0.21 Largemouth Bass Micropterus spp. UP 9/22/2020 337 566 0.23 0.14 0.32 0.21 Largemouth Bass Micropterus spp. UP 9/22/2020 309 433 0.17 0.18 0.36 0.21 Redear Sunfish Lepomis spp. DI 3/16/2020 230 218 0.27 0.11 0.48 0.22 Redear Sunfish Lepomis spp. DI 3/16/2020 188 110 0.21 0.06 0.36 0.21 Bluegill Lepomis spp. DI 9/17/2020 204 176 0.16 0.11 0.21 0.21 Bluegill Lepomis spp. DI 9/17/2020 154 64 0.19 < 0.06 0.68 0.2 Bluegill Lepomis spp. DI 9/17/2020 142 50 0.22 0.09 0.38 0.19 Redear Sunfish Lepomis spp. DI 9/17/2020 161 64 0.35 0.07 0.36 0.2 Largemouth Bass Micropterus spp. DI 3/16/2020 322 540 0.26 0.08 0.41 0.21 Largemouth Bass Micropterus spp. DI 3/16/2020 362 765 0.55 0.1 0.3 0.21 Largemouth Bass Micropterus spp. DI 3/16/2020 374 764 0.5 0.09 0.28 0.21 Largemouth Bass Micropterus spp. DI 3/16/2020 360 684 0.35 0.1 0.26 0.2 Largemouth Bass Micropterus spp. DI 3/16/2020 351 709 0.61 0.11 0.33 0.21 Largemouth Bass Micropterus spp. DI 3/16/2020 324 579 0.26 0.12 0.34 0.21 Redear Sunfish Lepomis spp. DW 9/22/2020 194 143 0.27 0.07 0.38 0.21 Bluegill Lepomis spp. DW 9/22/2020 163 90 0.17 0.08 0.22 0.19 Redear Sunfish Lepomis spp. DW 9/22/2020 192 142 0.33 0.07 0.31 0.22 Redear Sunfish Lepomis spp. DW 9/22/2020 182 120 0.31 0.06 0.33 0.21 Warmouth Lepomis spp. DW 9/22/2020 170 118 0.17 0.14 0.27 0.2 Warmouth Lepomis spp. DW 9/22/2020 201 197 0.16 0.34 0.2 0.19 Largemouth Bass Micropterus spp. DW 9/22/2020 354 660 0.26 0.11 0.28 0.2 Largemouth Bass Micropterus spp. DW 9/22/2020 333 612 0.43 0.1 0.28 0.21 Largemouth Bass Micropterus spp. DW 9/22/2020 322 518 0.27 < 0.06 0.3 0.2 Largemouth Bass Micropterus spp. DW 9/22/2020 391 1020 0.31 0.18 0.26 0.2 Largemouth Bass Micropterus spp. DW 9/22/2020 330 533 0.17 0.31 0.23 0.2 Largemouth Bass Micropterus spp. DW 9/22/2020 275 330 0.21 0.08 0.26 0.2 Bluegill Lepomis spp. Lake 6/22/2021 165 75 0.18 < 0.06 3.11 0.2 Redbreast Sunfish Lepomis spp. Lake 6/22/2021 186 116 0.18 < 0.06 2.28 0.2 Bluegill Lepomis spp. Lake 6/22/2021 182 121 0.19 < 0.06 2.57 0.21 Bluegill Lepomis spp. Lake 6/22/2021 176 113 0.21 < 0.06 2.2 0.2 Bluegill Lepomis spp. Lake 6/22/2021 173 97 0.17 < 0.06 2.86 0.2 Bluegill Lepomis spp. Lake 6/22/2021 158 77 0.18 < 0.06 2.34 0.21 Bluegill Lepomis spp. Lake 6/22/2021 162 78 0.2 < 0.06 1.93 0.19 Redear Sunfish Lepomis spp. Lake 6/23/2021 188 134 0.24 < 0.06 2.89 0.21 Bluegill Lepomis spp. Lake 6/23/2021 187 140 0.15 < 0.06 2.63 0.2 Bluegill Lepomis spp. Lake 6/23/2021 173 100 0.14 < 0.06 2.97 0.19 Largemouth Bass Micropterus spp. Lake 6/22/2021 282 264 0.15 < 0.06 2.1 0.2 Largemouth Bass Micropterus spp. Lake 6/22/2021 242 157 0.22 < 0.07 1.85 0.22 Largemouth Bass Micropterus spp. Lake 6/22/2021 330 453 0.16 < 0.06 2.7 0.2 9 Species Species group Location Collection Length Weight Date (mm) (g) Arsenic Mercury (As) (Hg) p0- Ngn, Dry to Selenium Wet (Se) µg/L Weight Ratio Largemouth Bass Micropterus spp. Lake 6/22/2021 271 258 0.21 < 0.07 2.14 0.22 Largemouth Bass Micropterus spp. Lake 6/22/2021 276 268 0.18 < 0.07 2.52 0.22 Largemouth Bass Micropterus spp. Lake 6/22/2021 280 263 0.25 < 0.07 1.79 0.22 Largemouth Bass Micropterus spp. Lake 6/22/2021 325 467 0.15 < 0.06 2.14 0.19 Largemouth Bass Micropterus spp. Lake 6/22/2021 253 209 0.21 < 0.07 1.87 0.22 Largemouth Bass Micropterus spp. Lake 6/22/2021 287 302 0.27 < 0.07 2.01 0.23 Largemouth Bass Micropterus spp. Lake 6/22/2021 266 260 0.2 < 0.07 2.03 0.22 Redear Sunfish Lepomis spp. UP 6/24/2021 230 218 0.18 0.09 0.47 0.21 Redear Sunfish Lepomis spp. UP 6/24/2021 215 222 0.18 0.14 0.37 0.21 Warmouth Lepomis spp. UP 6/24/2021 200 194 0.15 0.32 0.25 0.2 Redear Sunfish Lepomis spp. UP 6/23/2021 200 153 0.18 0.14 0.32 0.19 Redear Sunfish Lepomis spp. UP 6/23/2021 186 111 0.19 < 0.06 0.63 0.2 Bluegill Lepomis spp. UP 6/23/2021 180 140 0.17 0.09 0.2 0.21 Redear Sunfish Lepomis spp. UP 6/23/2021 199 148 0.21 < 0.06 0.4 0.21 Bluegill Lepomis spp. UP 6/23/2021 196 174 0.16 0.14 0.23 0.2 Warmouth Lepomis spp. UP 6/23/2021 171 131 0.16 0.17 0.29 0.19 Bluegill Lepomis spp. UP 6/24/2021 178 138 0.16 0.12 0.21 0.21 Largemouth Bass Micropterus spp. UP 6/23/2021 275 276 0.22 0.15 0.34 0.21 Largemouth Bass Micropterus spp. UP 6/23/2021 222 149 0.23 0.14 0.34 0.2 Largemouth Bass Micropterus spp. UP 6/23/2021 232 169 0.22 0.17 0.39 0.21 Largemouth Bass Micropterus spp. UP 6/23/2021 279 336 0.16 0.2 0.28 0.2 Largemouth Bass Micropterus spp. UP 6/23/2021 261 261 0.13 0.08 0.19 0.12 Largemouth Bass Micropterus spp. UP 6/23/2021 230 191 0.15 0.12 0.24 0.18 Largemouth Bass Micropterus spp. UP 6/23/2021 282 350 0.23 0.07 0.36 0.2 Largemouth Bass Micropterus spp. UP 6/23/2021 280 352 0.14 0.15 0.24 0.18 Largemouth Bass Micropterus spp. UP 6/23/2021 242 208 0.23 0.18 0.37 0.21 Largemouth Bass Micropterus spp. UP 6/23/2021 237 184 0.28 0.12 0.32 0.2 Bluegill Lepomis spp. DI 6/23/2021 200 186 0.16 0.19 0.22 0.2 Bluegill Lepomis spp. DI 6/23/2021 210 218 0.3 0.12 0.27 0.21 Redear Sunfish Lepomis spp. DI 6/23/2021 202 156 0.29 0.12 0.38 0.19 Bluegill Lepomis spp. DI 6/23/2021 184 137 0.16 0.12 0.28 0.2 Bluegill Lepomis spp. DI 6/23/2021 205 227 0.15 0.23 0.21 0.2 Bluegill Lepomis spp. DI 6/23/2021 205 211 0.18 0.13 0.24 0.22 Bluegill Lepomis spp. DI 6/23/2021 168 106 0.13 0.06 0.17 0.17 Bluegill Lepomis spp. DI 6/23/2021 195 184 0.23 0.11 0.21 0.16 Redear Sunfish Lepomis spp. DI 6/23/2021 202 151 0.11 0.05 0.31 0.12 Bluegill Lepomis spp. DI 6/23/2021 181 127 0.16 0.22 0.27 0.21 Largemouth Bass Micropterus spp. DI 6/23/2021 414 1096 0.16 0.19 0.2 0.14 Largemouth Bass Micropterus spp. DI 6/23/2021 402 1050 0.39 0.16 0.26 0.18 Largemouth Bass Micropterus spp. DI 6/23/2021 451 1238 0.32 0.33 0.32 0.21 Largemouth Bass Micropterus spp. DI 6/23/2021 445 1260 0.39 0.21 0.22 0.16 Largemouth Bass Micropterus spp. DI 6/23/2021 326 570 0.38 0.1 0.33 0.22 Largemouth Bass Micropterus spp. DI 6/23/2021 330 566 0.24 0.1 0.35 0.16 10 Species Species group Location Collection Length Weight Date (mm) (g) Arsenic Mercury (As) (Hg) µPS µl- Dry to Selenium Wet (Se) µg/L Weight Ratio Largemouth Bass Micropterus spp. DI 6/23/2021 335 534 0.26 0.16 0.4 0.21 Largemouth Bass Micropterus spp. DI 6/23/2021 330 558 0.17 0.22 0.29 0.21 Largemouth Bass Micropterus spp. DI 6/23/2021 360 714 0.29 0.25 0.42 0.22 Largemouth Bass Micropterus spp. DI 6/23/2021 423 1252 0.24 0.24 0.27 0.18 Largemouth Bass Micropterus spp. DW 9/15/2021 245 195 0.28 0.16 0.32 0.2 Largemouth Bass Micropterus spp. DW 9/15/2021 335 580 0.3 0.11 0.29 0.22 Largemouth Bass Micropterus spp. DW 9/15/2021 258 215 0.26 0.15 0.29 0.21 Largemouth Bass Micropterus spp. DW 9/15/2021 298 371 0.25 0.14 0.28 0.21 Largemouth Bass Micropterus spp. DW 9/15/2021 291 364 0.18 0.11 0.24 0.22 Largemouth Bass Micropterus spp. DW 9/15/2021 317 451 0.21 0.22 0.27 0.21 Largemouth Bass Micropterus spp. DW 9/15/2021 272 285 0.2 0.13 0.27 0.22 Largemouth Bass Micropterus spp. DW 9/15/2021 263 251 0.22 0.1 0.25 0.21 Largemouth Bass Micropterus spp. DW 9/15/2021 287 322 0.21 0.11 0.24 0.21 Largemouth Bass Micropterus spp. DW 9/15/2021 308 466 0.26 0.12 0.28 0.21 Redear Sunfish Lepomis spp. DW 9/15/2021 193 147 0.21 0.12 0.25 0.2 Redear Sunfish Lepomis spp. DW 9/15/2021 208 199 0.19 0.12 0.24 0.21 Redear Sunfish Lepomis spp. DW 9/15/2021 227 229 0.26 0.08 0.35 0.2 Bluegill Lepomis spp. DW 9/15/2021 208 233 0.19 0.1 0.23 0.23 Redear Sunfish Lepomis spp. DW 9/15/2021 215 228 0.18 0.09 0.27 0.21 Bluegill Lepomis spp. DW 9/15/2021 182 131 0.16 0.13 0.23 0.19 Redear Sunfish Lepomis spp. DW 9/15/2021 184 114 0.21 0.08 0.37 0.2 Bluegill Lepomis spp. DW 9/15/2021 186 141 0.19 0.12 0.25 0.2 Bluegill Lepomis spp. DW 9/15/2021 181 127 0.2 0.13 0.23 0.2 Bluegill Lepomis spp. DW 9/15/2021 175 102 0.19 0.1 0.24 0.2 *To convert to a dry weight, divide the fresh weight concentrations by the dry -to -wet weight ratio. 11