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Home My WebLink AboutI-9_Benthic Macroinvertebrate Monitoring Study Plans 2018-20222022 Benthic Macroinvertebrate Community Monitoring Study Plan -Final ntroduction As part of the City of Durham's National Pollutant Discharge Elimination System (NPDES) Stormwater Permit (NCS000249) [1], Section I: Water Quality Assessment and Monitoring, the City of Durham maintains an ambient water quality program aimed at assessing physical, chemical, and biological parameters at select sites in watersheds that drain from City jurisdictional areas. The majority of these sites are sampled on a rotating schedule each month of every other year with some sites sampled continuously as part of ongoing nutrient load monitoring efforts. Benthic macroinvertebrates are sampled on this odd/even year cycle using methods derived from standard operating procedures used by the North Carolina Department of Environmental Quality (NCDEQ) Biological Assessment Branch (BAB) at select sites. "Benthic macroinvertebrates" is a broad term applied to organisms from the mollusk, annelid, and arthropod phylum, usually found living near the bottom of aquatic environments. These invertebrates occupy numerous ecological niche roles in a hydrologic system, and are found in various substrates including submerged roots and leaf litter. Aquatic invertebrates, especially insects with an aquatic nymph stage, also make ideal indicators of overall stream health given their extended, sedentary life cycles (1-2 years), diversity, and commonness. Of particular importance are those belonging to the orders of Ephemeroptera, Plecoptera, and Trichoptera (EPT), due to their sensitivity to pollutants [2]. NCDEQ and other stakeholders have developed tolerance values (TV) associated with each taxon. These TV are further developed to yield five bioclassification ratings; Poor, Fair, Good -Fair, Good, and Excellent. With these bioclasses, it is possible to assess waterbodies across spatial and temporal differences. Streams with small drainage areas located mostly or entirely within the Triassic Basin may naturally be subject to flow cessation. Additionally, ideal habitat for aquatic invertebrates such as rocks and cobble typically found in other ecoregions, may be absent. Because of these natural conditions, the NCDEQ BAB typically designates stream sites with drainage areas smaller than 165 mi.' located in the Triassic Basin as "Not Rated" [2]. However, the City will assign bioclassifications to sites located in smaller Triassic Basin watersheds to monitor for spatial and temporal differences [3]. In the 2022 calendar year, a total of 7 sites will be sampled (Table 1, Figure 1) for benthic macroinvertebrates. A summary of CY2020 sampling data, from the selected sites is provided in Appendix I. City of Durham- WQU 2/25/2022 Figure 1. CY 2022 ambient and benthic monitoring sites. City of Durham- WQU 2/25/2022 Table 1. CY 2022 Benthic m acroin vertebrate community assessment sites. River Lat/Long Drainage Sampling basin Watershed Site code Stream Location (dec. deg.) Ecoregion area (mi.2) method 35.91102 Triassic Third Fork TFO.OTC Third Fork Cr. NC 751 16.1 Qua14 Q. M -78.96051 Basins u LL 35.8868 Triassic12.9 Northeast NEO.ONE Northeast Cr. Sedwick Rd. Qua14 -78 8998 Basins Johnson Mill 36.1416 Little LR9.6LR Little R. Slate Belt 78.2 Full Scale Rd. -78.9208 West Point on 36.0716 EN8.9ER Eno R. Slate Belt 141.1 Full Scale the Eno park -78.9100 a, Eno Penny's Bend 36.0731 ' a, EN4.9ER Eno R. Slate Belt 149.3 Full Scale z access -78.8635 Mineral Springs 35.9850 Triassic L. Lick LL3.4LLC Little Lick Cr. 6.0 Qua14 Rd. -78.8198 Basins 35.9777 Triassic Lick LC1.11-C Lick Cr. Southview Rd. 10.8 Qua14 -78.7496 Basins Methods Benthic macroinvertebrate sampling involves the collection of various fauna from a variety of instream habitats, and enumerating them either in the field or lab to the lowest possible taxonomic level. Next, a calculation is performed based on relative abundance and tolerance to stressors for each taxon which yields a bioclassification rating. The City of Durham currently uses two methodologies for the sampling and assessment of benthic macroinvertebrates; "Full Scale" and "Qual4" [3]. The method used depends on the drainage area of the stream and the ecoregion, as outlined below. • Full Scale: o Not in Triassic Basin Ecoregion and drainage area (DA) > 3 mi.' or in Triassic Basin Ecoregion and DA > 20 m i .2. o Sufficient diversity of habitat types are present. o Sampleable during summer season (June 1— August 31), unless seasonal corrections will be made. • Qual4: o Not in Triassic Basins ecoregion and DA < 3 mi.2 or in Triassic Basins ecoregion and DA < 20 mi.2. o Aquatic life use assessment not required. o Sampled during spring season (March 1— May 31). The Full Scale and Qua14 methods are similar in execution and target multiple substrates. However, there are some differences between the methods that are summarized below (Table 2). All benthic sampling will be conducted in accordance with the City of Durham's Standard Operating Procedures for Benthic Macroinvertebrate Community Monitoring, 2022 v. 1.0. City of Durham- WQU 2/25/2022 3 Table 2. Key Differences between Full scale and Qua14 methodologies. Sample Type Habitat Targeted # Samples for Full Scale # Samples for Qua14 Kick net Riffle 2 1 Sweep net Undercut banks, root mats, aquatic vegetation (macrophyte) beds 3 1 Leaf pack Decaying leaf packs (shredders) 1 1 Rock/log wash Rocks/logs in non -riffle areas 2 None Sand bag Depositional areas 1 None Visuals All potential habitats 10 min./person 10 min./ person Schedule & Responsibilities Benthic macroinvertebrate monitoring for CY 2022 will be coordinated by City of Durham Water Quality Unit staff in contract with Larry Eaton, President of Eaton Scientific, LS. Eaton Scientific should provide one additional staff member for benthic macroinvertebrate sampling efforts, with additional support as needed from City of Durham Stormwater & GIS staff. It is understood that staff from the City may have limited experience with benthic macroinvertebrate sampling and should not be expected to identify or enumerate benthic macroinvertebrates, but are capable of working in field conditions. Sites may be moved within a limited range based on the best professional judgement of the Eaton Scientific's team. City staff will provide field meters, forms, and digital cameras for these sampling events. Eaton Scientific will be responsible for all necessary field equipment required, as well as any additional materials and equipment necessary for the laboratory identification and analysis of samples. In accordance with standard operating outlined above Qua14 samples will be collected between March 1, 2022 and May 31, 2022, while Full Scale samples will be collected between June 1, 2022 and August 31, 2022. With results reported in an electronic format to the current Ambient Monitoring Program Project Manager at the earliest convenience. Further detail in relation to Eaton Scientific's requirements are contained in the Contract for Benthic Macroinvertebrate Community Assessment Services for The Stormwater Quality Program. 2021-2022 (CT# 17788). City of Durham- WQU 2/25/2022 References [1] City of Durham, Public Works Department, City of Durham Stormwater Management Plan (NCS000249), Durham, North Carolina: Clty of Durham, 2019. [2] NCDEQ, Standard Operating Procedures for the Collection and Analysis of Benthic Macroinvertebrates, V. 5.0, Raleigh, North Carolina: Division of Water Resources, 2016. [3] Stormwater & GIS Services, Water Quality Unit, Standard Operating Procedures for Benthic Macroinvertebrate Community Monitoring, Durham: City of Durham, 2022. City of Durham- WQU 2/25/2022 Appendix I- Results of CY 2018 Benthic Macroinvertebrates (Even Year) sites Appendix I z a �, Watershed siteDrainage Area Ecoregion 1 Sampling '8 E m m o= m m a m o a `—° A Identifier (4 mi j method 2$ i F F r roc m b 6 m u Third Fork TFO.OTC 16.1 TB Qual4 46 190 48 I 4 7.41 Poor a Northeast NE9.ONE 129 TB Qual4 46 106 49 2 7.54 Poor Little LR9.6LR 7&2 CSB Full Scale 89 283 84 28 4.96 Excellent EN8.9ER 141.1 CSB Full Scale 1 65 239 74 23 5.54 Good = Eno 80 31 Little Lick EN4.9ER 149-3 CSB Full Scale 76 273 90 2 5.13 6.98 Excellent Poor LL3.4LLC 6.0 TB Qua41 51 112 Lick LCJLKC 10.8 TB 9 5.80 Gaod-Fair Qual4 42 156 47 1 Level IV ecaregion, where TB = Triassic Basins; CSB = Carolina Slate Belt 2Qual4 (small streams) monitored in spring- Full Scale (rivers) monitored in summer. s Equivalent to EPT taxa richness (EPT S) S Binclassifiaation calculated using Full Scale (Large Streams} or Qual4 (Small Streams) criteria City of Durham- WQU 2/25/2022 6 Appendix II- Taxon and Tolerance Values of Collected Macroinvertebrates from the 2018 Sampling Season. Appendix II -Taxon and Tolerance Values of North Carolina Benthic Macroinvertebrates collected by City of Durham contractors in previous years. This list is not exhaustive of all Taxa found in North Carolina. Taxon Tolerance Value Taxon Tolerance Value Taxon Tolerance Value EPHEMEROPTERA DIPTERA; CHIRONOMIDAE ODONATA Familyeaetidoe Ablabesmyia mallochi 7.4 FamilyAeshnidae Plauditus dubius 2.2 Cladotanytarsus spp 4.0 Boyeria vinosa 5.6 Pseudocloeon propinquum 5.8 Cricotopus bicintus 8.7 Family Calopterygidae Family Caenidae Cricotopus/Orthocladius 3.4 Hetaerina spp 4.9 Caenis spp 6.8 Cricotopus vierriens group 5.4 Family Coenagrionidae Family Heptageniidae Cryptotendipes spp 6.2 Argia spp 8.3 Stenacron interpunctatum 6.4 Dicrotendipes simpsoni 9.8 Enallagma sp 8.5 PLECOPTERA Dicrotendipesmodestus 9.4 Family Corduliidae Family Nemouridae Dicrotendipes neomodestus 7.9 Neurocordulia obsoleta 5.3 Amphinemura spp 3.8 Diplocladius cultirger 8.0 Somatochlora spp 8.9 Prostoia spp 5.2 Hydobaenus spp 9.2 Family Gomphidae Family Perlidoe Kiefferulus dux Gomphus spp 5.9 Perlesta spp 2.9 Natarsia spp 9.6 Hagenius brevistylus 4.4 TRICHOPTERA Nilotanypus fimbratus 4.1 Stylogomphus albistylus 5.0 Family Hydropsychidae Orthocladius oliveri: 4.4 Family Libellulidae Cheumatopsyche spp 6.6 Orthocladius robacki 6.4 Perithemis spp 9.4 Hydropsyche betteni 7.9 Orthocladius rubicundus 5.7 Family Macromiidoe Family Limnephilidae Para. nigrohalteralis 4.9 Macromia spp 6.2 Ironoquia punctatissima 6.7 Phaenopsctra obediens 6.5 CRUSTACEA MISC DIPTERA Polypedilum halterale 7.4 Family Cambaridae Family5imuliidae Polypedilum fallax 6.5 immature crayfish 7.5 Simulium spp 4.9 Polypedilum flavum 5.7 Family Talitridae Family Tipulidae Polypedilum illinoense 8.7 Hyalella spp 7.2 Hexatoma spp 3.5 Polypedilum scalaenum 8.5 ORDER HEMIPTERA Tipula spp 7.5 Polypedilum tritum Metrobates spp OLIGOCHAETA Rheocricotopus robacki 4.7 Ranatra spp 6.3 Family Lumbriculidae 7.0 Rheotanytarsus spp 6.5 Rhagovelia spp Family Tubificidae Stelechomyiaperpulchrum 4.0 Rheumatobatesspp llyodrilus templetoni 9.3 Stenochironomus spp 6.3 Sigarra spp 8.7 Limnodrilus hoffineisteri 9.4 Stictochironomus devinctus 6.3 Synorthocladius spp 4.2 Thienemannimyia group 8.4 Tribelos jacundum 5.7 Tvetenia vitrces 3.5 City of Durham- WQU 2/25/2022 Appendix II- Taxon and Tolerance Values of Collected Macroinvertebrates from the 2018 Sampling Season. Taxon Tolerance Value Taxon Tolerance Value Taxon Tolerance Value CO LEO PTERA M O LLU SCA M EGALOPTE RA Family Dryopidae FamilyAncylidae Family Corydalidae Helichus spp 4.1 Ferrissia spp 6.6 Corydalus cornutus 5.2 Family Dytiscidae Family Hydrobiidae Nigronia serricornis 4.6 Neoporus mellitus 3.9 Amnicola spp 4.1 Family Sialidoe Family Elmidae Family Physidae Sialis spp 7.0 Dubiraphia vittata 5.0 Physa spp 8.7 OTHER TAXA Macronychus glabratus 4.7 Planorbidae Family Glossihoniidae Microcylloepeus spp 3.3 Helisoma anceps 6.6 Desserobdella phalera 6.6 Tanytarsus gibbus 6.6 Family Pleuroceridae Placobdella ornata Tanytarsus sp 0 6.6 Elimia spp 2.7 Family Planariidae Thienemaniella spp 6.4 Leptoxis spp 1.7 Dugesia tigrina 7.1 Stenelmis spp 5.6 Family Viviparidoe Family Pyralidae Family Hydrophilidae Campelomadecisum 5.8 Family Tetrastemmatidae Sperchopsis tessalatus 4.4 Family Corbiculidae Prostoma graecens 6.6 Family Psephenidae Corbicula fluminea 6.6 Family Hydrachnidae Psephenus herricki 2.3 Family Sphaeriidoe Hydracarina spp 5.5 City of Durham- WQU 2/25/2022 PUBLIC WORKS CITY OF DURHAM CY21 Benthic Macroinvertebrate Monitoring Study Plan Joseph Smith, Environmental Planning & Compliance Analyst City of Durham, Department of Public Works, Stormwater & GIS Division March 11, 2021 Introduction As part of the City of Durham's National Pollutant Discharge Elimination System (NPDES) Stormwater Permit (NCS000249), the City of Durham maintains an ambient water quality program aimed at assessing physical, chemical, and biological parameters at select sites in watersheds that drain from City jurisdictional areas. Benthic macroinvertebrates are sampled annually using methods derived from standard operating procedures used by the North Carolina Department of Environmental Quality (NCDEQ) Biological Assessment Branch. "Benthic macroinvertebrates" is a broad term applied to organisms from the mollusk, annelid, and arthropod phylum, usually found living near the bottom of aquatic environments. These invertebrates occupy numerous ecological niche roles in a hydrologic system, and are found in various substrates including submerged roots and leaf litter. Aquatic invertebrates, especially insects with an aquatic nymph stage, also make ideal indicators of overall stream health given their extended, sedentary life cycles (1-2 years), diversity, and commonness. Of particular importance are those belonging to the orders of Ephemeroptera, Plecoptera, and Trichoptera (EPT), due to their sensitivity to pollutants. NCDEQ and other stakeholders have developed tolerance values (TV) associated with each taxon. These TV are further developed to yield 5 bioclassification ratings; Poor, Fair, Good -Fair, Good, and Excellent. Of note, small catchments in the Triassic Basin are usually not rated by the State due to the hydrogeology which is characterized by periods of flow cessation, and lack of suitable habitat to colonize [1]; consequently, sampling by the City in small catchments in the Triassic Basin is only used to assess the bioclassification of aforementioned streams within city limits in comparison to one another. In the 2021 calendar year, a total of 6 sites will be sampled (Table 1, Figure 1) for benthic macroinvertebrates. As part of the 2021 Ambient Monitoring Study Plan, sampling at PN2.4PN Panther Creek at Burton Road was discontinued, PN1.9PN (Panther Creek at Cooksbury Drive) located downstream was selected to replace it. For the 2021 Benthic Macroinvertebrate Monitoring Study sampling will be conducted at this new location and sampling at PN2.4PN will be discontinued. A summary of results, including CY2019 sampling data, from the selected sites is provided in Appendix I. 101 City Hall Plaza, Third Floor, Durham, NC 27701 I 919.560.4326 I DurhamNC.gov Follow Us @Cit o© rhamNNCC Person Con - Durham GpuM-. I £t 2021 Denthic Sites USGS rlvwand Stage Gages + USGS Stage Duly Gages 41 _ • NPDES Swrntwater, Individual r y ■ NPDES Swrmwatar, (,aneral ! ■ NPDES Waatexater Pamata % s DWR Ambient M-Wng Shea UCFRSACaalftionSR- ■ M&AMonaa nug Stations EEl SDWREL.sd"- n Ri-8-in U — � NNII¢rlt LOadfng Spec 20191mpaVred Streams S lorexo C9 / I CRy of Durham 5exersheds _ f JC3 Durham County •,`�1 I� ! r 4 -hl EN8AER :ts'e ec Itise.4��tN — PN2.441 r _ l EL9.9EC 1 I ! �+ EL7.1ECEL6.6G'C f r` ■• lf ' ELB.BSEC EL7.15EC ■ ve Fear 1' L VI ELB:BSECITf ■ �' - NH4.9SC —6 I I NH4.45CTD ! — NH9.38C LL9.iLLC, J/{ NH2:9MC NH1.73CTA roTc; `. ti °� .. CC2.scc Durh msC unry Jp _J Chat Coun " 2021 r,fomonmidey��iss 5eav6.dr.5anme-C'u1 ofPMuo tMl'. N�DWRAAL, mr�Ema«�amayaary.� mut wman ernw ue� rml�r.'swwrr�m��i9,,m�.�wso®i®.pxnum m,x. Miles Ambient Monitoring -- siwes-TFe ry'ar nEAAamaT�M1•m e�.oyy wwr�ru NPDF:sPo na NC nEAa,R aoiau tarvoF "�'�' 01018� '� "c°`"�'6Or ^w Mre.sa�a..ww.e,e�aiu-Nlmzoi r�-� 0 0.5 1 2 3 Sites OURNAM FIGURE 1. CY 2021 AMBIENT AND BENTHIC MONITORING SITES. 101 City Hall Plaza, Third Floor, Durham, NC 27701 919.560.4326 I DurhamNC.gov Follow Us @C© ofDurhamNC TABLE 1. CY 2021 BENTHIC MONITORING SITES. River basin Watershed Site code Stream Location Latitude (dd.ddd) Longitude (dd.ddd) Sampling method Drainage area (mi?) Cape Fear New Hope NH2.3MC Mud Cr Pickett Rd 35.9753 -78.9844 Qual4 5.4 NHI.OSC Sandy Cr Garrett Rd 35.9571 -78.9738 Qual4 6.9 Neuse Ellerbe EL1.9EC Ellerbe Cr Glenn Rd 36.0596 -78.8327 Full Scale 21.9 EL5.5GC Goose Cr Camden Ave 36.0248 -78.8609 Qual4 6.4 EL7.1EC Ellerbe Cr Acadia St 36.0200 -78.8952 Qual4 5.7 Panther PN1.9PN Panther Cr Cooksbury Dr. 36.0369 -78.8064 Qual4 2.6 Methods Benthic macroinvertebrate sampling involves the collection of various fauna from a variety of instream habitats, and enumerating them either in the field or lab to the lowest taxonomic level. Next, a calculation is performed based on relative abundance and tolerance to stressors for each taxon which yields a bioclassification rating. The City of Durham currently uses two methodologies for the sampling and assessment of benthic macroinvertebrates; the "Full Scale' and "Qual4" [1]; however, the criteria to assign a methodology to a particular site has been modified as listed in the City of Durham's Standard Operating Procedure [2]. This modification of the method is due to the prevalence of small watersheds with Triassic Basin soils located within the City of Durham. Standard NC Department of Environmental Quality methodology requires Triassic basins to have at least 165 square miles of drainage area, otherwise they are given the "Not Rated" classification [1]; by modifying this approach, the City is able to determine a bioclassification of small-scale Triassic streams. The determination of what methodology is used at each sampling site is summarized below: • Full Scale: o Not in Triassic Basins ecoregion and drainage area (DA) > 3 mi.z or in Triassic Basins ecoregion and DA > 20 mi.z. o Sufficient diversity of habitat types are present. o Sampleable during summer season (June 1- August 31), unless seasonal corrections will be made. • Qual4: o Not in Triassic Basins ecoregion and DA < 3 mi.z or in Triassic Basins ecoregion and DA < 20 mi.2. o Aquatic life use assessment not required. o Sampled during spring season (March 1 - May 31). The Full Scale and Qua14 methods are similar in execution and target multiple substrates. However, there are some differences between the methods that are summarized below (Table 2). All benthic sampling will be conducted in accordance with the City of Durham's Standard Operating Procedures for Benthic Macroinvertebrate Community Monitoring- Draft, 2019 v. 1.0, as well as NC Department of Environmental Quality's Standard Operating Procedures for the Collection and Analysis of Benthic Macroinvertebrates, 2016 v5.0. TABLE 2. KEY DIFFERENCES BETWEEN FULL SCALE AND QUAL4 METHODOLOGIES. Sample Type Habitat Targeted # Samples for Full Scale # Samples for Qua14 Kick net Riffle 2 1 Sweep net Undercut banks, root mats, aquatic vegetation macro h e beds 3 1 Leafpack Deca ing leaf packs shredders 1 1 Rock/log wash Rocks/logs in non -riffle areas 2 None Sand bag Depositional areas 1 None Visuals All potential habitats 10 min./person 10 min.person 101 City Hall Plaza, Third Floor, Durham, NC 27701 919.560.4326 I DurhamNC.gov Follow Us @C© ofDurhamNC �� t� Schedule & Responsibilities Benthic macroinvertebrate monitoring for CY 2021 will be coordinated by City of Durham Water Quality Unit staff in contract with Larry Eaton, President of Eaton Scientific, LS. Eaton Scientific should provide one additional staff member for benthic macroinvertebrate sampling efforts, with additional support as needed from City of Durham Stormwater & GIS staff. It is understood that staff from the City may have limited experience with benthic macroinvertebrate sampling and should not be expected to identify or enumerate benthic macroinvertebrates, but are capable of working in field conditions. Sites may be moved within a limited range based on the expert advice from members of the Eaton Scientific's team. City staff will provide field meters, forms, and digital cameras for these sampling events. Eaton Scientific will be responsible for all necessary field equipment required, as well as any additional materials and equipment necessary for the laboratory identification and analysis of samples. In accordance with standard operating procedures put forth by NCDEQ, Qua14 samples will be collected between March 1, 2021 and May 31, 2021, while Full Scale samples will be collected between June 1, 2021 and August 31, 2021. With results reported in an electronic format to the current Ambient Monitoring Program Project Manager by November 1st, 2 02 1. Further detail in relation to Eaton Scientific's requirements are contained in the Contract for Benthic Macroinvertebrate Community Assessment Services for the Stormwater Quality Program, 2021-2022 (Contract # 17788). 101 City Hall Plaza, Third Floor, Durham, NC 27701 919.560.4326 I DurhamNC.gov Follow Us @C© ofDurhamNC �� t� References [1] NCDEQ, Standard Operating Procedures for the Collection and Analysis of Benthic Macroinvertebrates, V. 5.0, Raleigh, North Carolina: Division of Water Resources, 2016. [2] City of Durham, Stormwater & GIS Servicies, Standard Operating Procedures for Benthic Macroinvertebrate Community Monitoring for Determination of Bioclassiflications, DRAFT, v. 1.0, Durham, North Carolina: Water Quality Unit, 2019. [3] City of Durham, Public Works Department, City of Durham Stormwater Management Plan (NCS000249), Durham, North Carolina: CIty of Durham, 2019. 101 City Hall Plaza, Third Floor, Durham, NC 27701 I 919.560.4326 I DurhamNC.gov Follow Us @Cit o© rhamNNCC Appendix I- Summary of Benthic Sampling Results from 2007 to 2019 Results from 2007- 2019 benthic sampling where: S = total # taxa; N = total # individuals; EPT S = total # EPT taxa; EPT N = total # EPT individuals; BI = biotic index; WQ rating = final bio classification. Samples that had a WQ Rating that was missing or "Not Rated" have been excluded. Date S N rating 3/19/2008 26 74 5 16 8.27 Poor 3/19/2008 30 109 5 - 7.74 Poor 2/12/2009 51 204 5 25 7.61 Poor 9/16/2009 37 106 7 45 6.89 Fair 9/16/2009 48 158 7 48 7.18 Fair EL1.9EC 7/1/2010 64 - 8 32 6.87 Fair 6/17/2011 45 6 26 6.91 Fair 6/13/2013 28 3 - 7.3 Poor 2015 29 90 6 - 5.92 Good/Fair 7/17/2017 56 240 12 - 6.85 Fair 6/26/2019 65 268 11 64 6.99 Fair 3/19/2008 13 30 0 0 8.52 Poor 3/19/2008 18 83 0 0 8.44 Poor 2/11/2009 22 79 2 11 7.55 Poor 2/11/2009 29 125 2 20 7.82 Poor EL5.5GC 4/8/2011 36 - 3 - 7.72 Poor 4/26/2013 35 - 3 - 7.9 Poor 4/8/2015 16 90 3 - 7.01 Poor 3/30/2017 42 154 3 - 7.28 Poor 5/10 2019 36 114 4 22 7.08 Poor 7/9/2007 30 98 5 22 7.09 Poor 3 10 2009 35 137 4 13 7.62 Poor 3 10 2009 43 192 5 17 7.62 Poor 4 29 2010 53 235 4 24 7.75 Poor NHI.OSC 8 16 2010 49 - 6 21 7.61 Poor 4 14 2011 23 4 - 7.52 Poor 4 25 2013 48 5 - 7.4 Poor 4 7 2015 16 55 2 - 6.85 Fair 3 30 2017 41 95 4 - 7.27 Poor 5 10 2019 29 89 5 25 6.83 Fair 3 9 2009 55 207 6 24 7.61 Poor 4 28 2010 59 215 9 51 6.35 Fair 4 15 2011 28 - 10 32 6.34 Fair NH2.3MC 4 25 2013 53 - 7 - 6.8 Fair 4 7 2015 26 75 5 - 5.17* Good* 3 30 2017 64 194 12 - 6.61 Good -Fair 5 10 2019 57 185 9 37 6.52 Fair 2 9 2009 39 169 8 31 7.01 Fair 5 4 2010 50 145 5 29 7.41 Poor 3 21 2011 28 - 3 - 7.64 Poor PN2.4PN 5 16 2013 41 - 3 - 6.3 Fair 4 7 2015 29 77 6 - 5.72 Good -Fair 3 30 2017 41 121 6 - 5.88 Fair 5/10/2019 40 140 9 51 5.64 Good -Fair 101 City Hall Plaza, Third Floor, Durham, NC 27701 I 919.560.4326 I DurhamNC.gov I Follow Us @CO o© rhamNNCC Appendix II- Taxon and Tolerance Values of Collected Macroinvertebrates from the 2019 Sampling Season. Appendix II -Taxon and Tolerance Values of North Carolina Benthic Macroinvertebrates collected by City of Durham contractors in 2019. This list is not exhaustive of all Taxa found in North Carolina. Taxon TV Taxon TV Taxon TV EPHEMEROPTERA MISC DIPTERA DIPTERA; CHIRONOMIDAE, cont. Family Baetidae Family Ceratopogonidae Paratanytarsus spp 8 Baetis flavistriga 6.8 Palpomyia complex 5.7 Paratendipes subaequalis 5.6 Baetis intercalaris 5 Family Culicidae Polypedilum aviceps 3.6 Baetis pluto 3.4 Anopholes spp 8.6 Polypedilum flavum 5.7 Plauditus dubius 2.2 Culex spp Polypedilum halterale 7.4 Plauditus punctiventris Family Dixidae Polypedilum illinoense 8.7 Procloeon spp 1.9 Dixella spp 4.7 Polypedilum scalaenum 8.5 Pseudocloeon propinquum 5.8 Family Simuliidae Polypedilum tritum Family Caenidae Simulium spp 4.9 Procladius spp 8.8 Caenis spp 6.8 Family Tipulidae Rheocricotpus robacki 7.9 Family Heptageniidae Antocha spp 4.4 Rheotanytarsus spp 6.5 Stenacron interpunctatum 6.4 Tipula spp 7.5 Smittia PLECOPTERA DIPTERA; CHIRONOMIDAE Stenochironomus spp 6.3 Family Nemouridae Ablabesmyia mallochi 7.4 Synorthocladius 4.2 Amphinemoura 3.8 Brillia flavifrons 5.7 Tanytarsus acifer 6.6 Family Perlidae Chironomus spp 9.3 Tanytarsus buckleyi 6.6 Perlesta spp 2.9 Corynoneura spp 5.7 Tanytarsus sp G 6.6 TRICHOPTERA Cladotanytarsus sp E 4 Thienemaniella spp 6.4 Family Hydropsychidae Cladotanytarsus viridiventris 4 Thienemannimyia group 8.4 Cheumatopsyche spp 6.6 Cricotopus bicintus (C/O sp 1) 8.7 Tribelos jacundum 5.7 Hydropsyche betteni 7.9 Cryptochironomus fulvus 6.7 Tvetenia bavarica gp (E sp 1) 3.6 Family Leptoceridae Cryptotendipes spp 6.2 Tvetenia vitraces 3.5 Triaenodes ignitus 4.6 Diamesa spp 6.6 COLEOPTERA Family Philopotamidae Dicrotendipes neomodestus 7.9 Family Dryopidae Chimarra spp 3.3 Eukieferiella claripennis 6.2 Helichus fastigiatus 4.1 Kiefferulus dux Helichus lithophagus 3 Nilotanypus fimbratus 4.9 Family Dytiscidae Orthocladius robacki (C/O sp 12) 6.4 Neoporus mellitus 3.9 Paracladopelma spp 6.3 Neoporus spp 5 Parametriocnemus lundbecki 3.7 Family Elmidae Dubiraphia spp 5 Macronychus glabratus 4.7 Stenelmis spp 5.6 Family Noteridae Hydrocanthus spp 101 City Hall Plaza, Third Floor, Durham, NC 27701 919.560.4326 I DurhamNC.gov I Follow Us @CiittyofDurhamNC Appendix II- Taxon and Tolerance Values of Collected Macroinvertebrates from the 2019 Sampling Season. Taxon TV Taxon TV Taxon TV ODONATA MOLLUSCA OTHER TAXA Family Aeshnidae Family Ancylidae Family Irpobdellidae Boyeria vinosa 5.6 Ferrissia spp 6.6 Erpobdella/Mooreobdella spp 8.6 Family Calopterygidae Family Hydrobiidae Family Glossihoniidae Calopteryx spp TS Family Physidae Desserobdella phalera 6.6 Family Coenagrionidae Physa spp 8.7 Desserobdella picta Argia spp 8.3 Family Planorbidae Family Planariidae Enallagma sp 8.5 Mentus dilatus 7.6 Dugesia tigrina 7.1 Family Corduliidae Family Corbiculidae Family Hydrachnidae Somatochlora spp 8.9 Corbicula fluminea 6.6 Hydracarina spp 5.5 Family Gomphidae HETEROPODA Spongilla spp Progomphus obscurus 8.2 Belostoma spp 9.5 Family Hydrachnidae Family Libellulidae Ranatra spp 6.3 Hydracarina spp 5.5 Pachydiplax longipenis 9.6 Sigarra spp 8.7 OLIGOCHAETA Family Lumbriculidae 7 Family Naidae Pristinella spp 7.7 Pristinella spp 7.7 Slavina appendiculata 8.4 Family Tubificidae Limnodrilus hoffineisteri 9.4 CRUSTACEA Family Asellidae Caecidotea spp 8.4 Family Cambaridae immature crayfish TS 101 City Hall Plaza, Third Floor, Durham, NC 27701 1 919.560.4326 1 DurhamNC.gov I Follow Us @CityofDurhamNC f �. PUBLIC WORKS CITY OF DURHAM February 4, 2020 MEMO To: Michelle Woolfolk, Sr. Environmental Planning and Compliance Manager, COD JV Loperfido, Environmental Planning and Compliance Manager, COD Larry Eaton, President, Eaton Scientific From: Joseph Smith, Environmental Planning and Compliance Analyst, COD Subject: CY20 Benthic Macroinvertebrate Monitoring Study Plan Introduction As part of the City of Durham's National Pollutant Discharge Elimination System (NPDES) Stormwater Permit (NCS000249), Section I: Water Quality Assessment and Monitoring, the City of Durham maintains an ambient water quality program aimed at assessing physical, chemical, and biological parameters at select sites in watersheds that drain from City jurisdictional areas. The majority of these sites are sampled on a rotating schedule each month of every other year with some sites sampled continuously as part of ongoing nutrient load monitoring efforts. In conjunction with the ongoing monthly ambient chemistry sampling, benthic macroinvertebrates are sampled annually using methods derived from standard operating procedures used by the North Carolina Department of Environmental Quality (NCDEQ) Biological Assessment Branch. "Benthic macroinvertebrates" is a broad term applied to organisms from the mollusk, annelid, and arthropod phylum, usually found living near the bottom of aquatic environments. These invertebrates occupy numerous ecological niche roles in a hydrologic system, and are found in various substrates including submerged roots and leaf litter. Aquatic invertebrates, especially insects with an aquatic nymph stage, also make ideal indicators of overall stream health given their extended, sedentary life cycles (1-2 years), diversity, and commonness. Of particular importance are those belonging to the orders of Ephemeroptera, Plecoptera, and Trichoptera (EPT), due to their sensitivity to pollutants. NCDEQ and other stakeholders have developed tolerance values (TV) associated with each taxon. These TV are further developed to yield 5 bioclassification ratings; Poor, Fair, Good -Fair, Good, and Excellent. With these bioclasses, it is possible to assess waterbodies across spatial and temporal differences. Of note, small catchments in the Triassic Basin are usually not rated by the State due to the hydrogeology which is characterized by periods of flow cessation, and lack of suitable habitat to colonize [1]; consequently, sampling by the City in small catchments in the Triassic Basin is only used to assess the bioclassification of aforementioned streams within city limits in comparison to one another. In the 2020 calendar year, a total of 7 sites will be sampled (Table 1, Figure 1) for Benthic macroinvertebrates. A summary of results, including CY2018 sampling data, from the selected sites is provided in Appendix I. 101 City Hall Plaza, Third Floor, Durham, NC 27701 919.560.4326 I DurhamNC.gov Follow Us @CityofDurhamNC 0 FIGURE 1. CY 2020 AMBIENT AND BENTHIC MONITORING SITES. 101 City Hall Plaza, Third Floor, Durham, NC 27701 1 919.560.4326 1 DurhamNC.gov I Follow Us @CityofDurhamNC TABLE 1. CY 2020 BENTHIC MONITORING SITES. Lat/Long Drainage River Site Sampling basin Watershed code Stream Location (dec. Ecoregion area method de mi.z 35.91102 Triassic Third Fork TFO.OTC Third Fork Cr. NC 751 16.1 Qual4 ¢ -78.96051 Basins Northeast NEO.ONE Northeast Cr. Sedwick Rd. 35.8868 Triassic 12.9 Qual4 -78.8998 Basins Johnson Mill 36.1416 Little LR9.6LR Little R. Slate Belt 78.2 Full Scale Rd. -78.9208 EN8.9ER Eno R. West Point on 36.0716 Slate Belt 141.1 Full Scale the Eno ark -78.9100 y Eno Penny's Bend 36.0731 EN4.9ER Eno R. Slate Belt 149.3 Full Scale z access -78.8635 Mineral 35.9850 Triassic L. Lick LL3.4LLC Little Lick Cr. 6.0 Qua14 Springs Rd. -78.8198 Basins 35.9777 Triassic Lick LC1.1LC Lick Cr. Southview Rd. 10.8 Qua14 -78.7496 Basins MTS!ro0? Benthic macroinvertebrate sampling involves the collection of various fauna from a variety of instream habitats, and enumerating them either in the field or lab to the lowest taxonomic level. Next, a calculation is performed based on relative abundance and tolerance to stressors for each taxon which yields a bioclassification rating. The City of Durham currently uses two methodologies for the sampling and assessment of benthic macroinvertebrates; the "Full Scale" and "Qual4" [1]; however, the criteria to assign a methodology to a particular site has been modified as listed in the City of Durham's Standard Operating Procedure [2]. This modification of the method is due to the prevalence of small watersheds with Triassic Basin soils located within the City of Durham. Standard NC Department of Environmental Quality methodology requires Triassic basins to have at least 165 square miles of drainage area, otherwise they are given the "Not Rated" classification [1]; by modifying this approach, the City is able to determine a bioclassification of small-scale Triassic streams. The determination of what methodology is used at each sampling site is summarized below: • Full Scale: o Not in Triassic Basins ecoregion and drainage area (DA) > 3 mi.z or in Triassic Basins ecoregion and DA > 20 mi.z. o Sufficient diversity of habitat types are present. o Sampleable during summer season (June 1 - August 31), unless seasonal corrections will be made. • Qual4: o Not in Triassic Basins ecoregion and DA < 3 mi.z or in Triassic Basins ecoregion and DA < 20 mi.z. o Aquatic life use assessment not required. o Sampled during spring season (March 1 - May 31). The Full Scale and Qua14 methods are similar in execution and target multiple substrates. However, there are some differences between the methods that are summarized below (Table 2). All benthic sampling will be conducted in accordance with the City of Durham's Standard Operating Procedures for Benthic Macroinvertebrate Community Monitoring- Draft, 2019 v. 1.0, as well as NC Department of Environmental Quality's Standard Operating Procedures for the Collection and Analysis of Benthic Macroinvertebrates, 2016 v5.0. 101 City Hall Plaza, Third Floor, Durham, NC 27701 I 919.560.4326 I DurhamNC.gov I Follow Us @CO o© rhamNNCC TABLE 2. KEY DIFFERENCES BETWEEN FULL SCALE AND QUAL4 METHODOLOGIES. Sample Type Habitat Targeted # Samples for Full Scale # Samples for Qua14 Kick net Riffle 2 1 Sweep net Undercut banks, root mats, aquatic vegetation macro h to beds 3 1 Leafpack Decaying leaf packs shredders 1 1 Rock/log wash Rocks/logs in non -riffle areas 2 None Sand bag Depositional areas 1 None Visuals All potential habitats 10 min./person 10 min. erson Schedule & Responsibilities Benthic macroinvertebrate monitoring for CY 2020 will be coordinated by City of Durham Water Quality Unit staff in contract with Larry Eaton, President of Eaton Scientific, LS. Eaton Scientific should provide one additional staff member for benthic macroinvertebrate sampling efforts, with additional support as needed from City of Durham Stormwater & GIS staff. It is understood that staff from the City may have limited experience with benthic macroinvertebrate sampling and should not be expected to identify or enumerate benthic macroinvertebrates, but are capable of working in field conditions. Sites may be moved within a limited range based on the expert advice from members of the Eaton Scientific's team. Of particular interest is the reassessment of the site located in the Lick Creek Watershed, LC1.1LC. Given its historically Fair to Poor rating; this site has relatively poor habitat for benthic macroinvertebrates, and may not be a suitable location due more to hydrogeology and less to upstream anthropological impacts. City staff will provide field meters, forms, and digital cameras for these sampling events. Eaton Scientific will be responsible for all necessary field equipment required, as well as any additional materials and equipment necessary for the laboratory identification and analysis of samples. In accordance with standard operating procedures put forth by NCDEQ, Qua14 samples will be collected between March 1, 2020 and May 31, 2020, while Full Scale samples will be collected between June 1, 2020 and August 31, 2020. With results reported in an electronic format to the current Ambient Monitoring Program Project Manager at the earliest convenience. Further detail in relation to Eaton Scientific's requirements are contained in the CY 2020 Amended "Contract for Benthic Macroinvertebrate Monitoring Services in Support of Durham's Ambient Monitoring Program for Calendar Years 2017 and 2018" dated February 24, 2017 (Contract #14294). 101 City Hall Plaza, Third Floor, Durham, NC 27701 I 919.560.4326 I DurhamNC.gov I Follow Us @CO o© rhamNNCC References [1] NCDEQ Standard Operating Procedures for the Collection and Analysis of Benthic Macroinvertebrates, V. 5.0, Raleigh, North Carolina: Division of Water Resources, 2016. [2] City of Durham, Stormwater & GIS Servicies, Standard Operationg PRocedures for Benthic Macroinvertebrate Community Monitoring for Determination of Bioclassifications, DRAFT, v. 1.0, Durham, North Carolina: Water Quality Unit, 2019. [3] City of Durham, Public Works Department, City of Durham Stormwater Management Plan (NCS000249), Durham, North Carolina: City of Durham, 2019. 101 City Hall Plaza, Third Floor, Durham, NC 27701 I 919.560.4326 I DurhamNC.gov I Follow Us @CO o© rhamNC Appendix I- Results of CY 2018 Benthic Macroinvertebrates (Even Year) sites Appendix I Results from 2002- 2018 benthic sampling where: S = total # taxa; N = total # individuals; EPT S = total # EPT taxa; EPT N = total # EPT individuals; BI = biotic index; WQ rating = final bio classification. Samples that had a WQ Rating that was missing or "Not Rated" have been excluded. Site ID Collection D. 8/6/2002 34 174 17 95 5.46 Good 8/28/2003 48 218 15 122 5.89 Good -Fair 6/23/2004 52 -- 14 87 5.82 Good -Fair 6/17/2005 43 204 15 95 5.72 Good - Fair 6/21/2006 63 211 18 90 6.50 Good -Fair 6/29/2007 53 195 20 99 5.76 Good -Fair EN4.9ER 6/9/2008 57 237 19 110 5.94 Good -Fair Full Scale 6/23/2009 51 261 17 147 5.86 Good -Fair 6/29/2010 51 212 23 146 5.37 Good 6/16/2011 81 27 139 5.96 Good 7/19/2012 56 -- 20 111 6.08 Good -Fair 8/7/2014 46 177 22 5.28 Good 7/14/2016 49 175 23 4.94 Good 7/6/2018 73 273 3.0 118 5.59 Good -Fair 5/30/2002 55 265 21 116 5.30 Good 6/24/2003 43 190 12 65 6.24 Good -Fair 6/22/2004 44 181 18 100 5.22 Good 6/13/2005 56 -- 19 115 5.20 Good 6/20/2006 50 195 18 78 5.87 Good -Fair EN8.9ER 6/19/2007 69 253 30 123 5.72 Good 6/10/2008 55 193 21 82 6.03 Good -Fair Full Scale 6/24/2009 62 252 18 100 5.70 Good -Fair 6/28/2010 59 219 18 92 5.92 Good -Fair 6/14/2011 63 -- 17 80 5.97 Good -Fair 8/7/2014 51 254 20 5.10 Good 6/23/2016 45 164 20 5.26 Good 7/6/2018 72 286 21 133 5.12 Good 7/15/2004 46 -- 7 24 7.09 Fair 6/16/2005 27 112 6 14 7.43 Fair 8/15/2006 37 131 10 48 6.67 Fair 7/9/2007 22 41 4 10 7.15 Poor LC1.1LC 3/14/2008 23 96 6 28 7.35 Fair 6/30/2009 56 233 8 7.06 Fair Qual4 4/20/2010 44 163 8 66 6.11 Fair 7/2/2010 71 -- 7 40 7.18 Fair 5/9/2012 52 -- 9 35 6.97 Fair 5/8/2014 21 60 6 6.35 Fair 4/7/2016 29 91 10 5.62 Fair 7/6/2018 27 88 7 29 5.78 Good -Fair 101 City Hall Plaza, Third Floor, Durham, NC 27701 I 919.560.4326 I DurhamNC.gov Follow Us @CO o© rhamNC Appendix I- Results of CY 2018 Benthic Macroinvertebrates (Even Year) sites Results from 2002- 2018 benthic sampling where: S = total # taxa; N = total # individuals; EPT S = total # EPT taxa; EPT N = total # EPT individuals; BI = biotic index; WQ rating = final bio classification. Samples that had a WQ Rating that was missing or "Not Rated" have been excluded. 8/1/2002 20 97 2 20 8.07 Poor 9 3 2003 31 133 4 13 8.02 Poor 7 22 2004 29 110 1 10 7.29 Poor 6 15 2005 23 142 1 10 7.46 Poor 8 24 2006 38 136 2 4 8.35 Poor 6/12/2008 41 135 2 13 8.25 Poor LL3.4LLC 6/12/2008 32 92 2 4 8.26 Poor Qua14 2/16/2009 41 133 4 8 7.96 Poor 2/16/2009 35 108 4 8 7.77 Poor 4/13/2011 35 -- 3 -- 7.99 Poor 5/9/2012 52 -- 5 25 7.62 Poor 5/8/2014 29 63 3 3 7.22 Poor 4/7/2016 18 44 3 -- 6.03 Poor 3/28/2018 13 50 1 10 7.66 Poor 8/25/2004 77 -- 29 168 5.33 Good 6/20/2005 63 315 29 155 5.09 Excellent 8 2 2006 86 320 24 141 5.6 Good 8 21 2007 80 342 18 107 5.84 Good -Fair 6 17 2008 72 257 24 120 5.59 Good LR9.6LR 7 9 2009 66 314 25 152 5.76 Good Full Scale 8/19/2010 62 -- 20 138 5.45 Good -Fair 8/15/2012 78 -- 27 161 5.58 Good 8/6/2014 68 254 27 -- 4.91 Good 6/23/2016 50 217 25 -- 4.91 Good 7/6/2018 93 403 27 167 5.15 Good 6 25 2002 43 166 9 34 7.55 Poor 7 15 2003 37 140 5 34 7.16 Poor 8 10 2004 33 -- 5 23 7.57 Poor 7/11/2005 38 190 4 33 7.57 Poor NEO.ONE 9/21/2006 34 130 3 9 7.92 Poor Qua14 8/2/2007 36 -- 3 -- 7.85 Poor 5/8/2014 18 43 2 7.89 Poor 4/7/2016 19 69 2 7.03 Poor 3/28/2018 28 67 1 3 7.31 Poor 8/14/2002 37 190 3 14 8.08 Poor 9/11/2003 34 157 4 24 7.26 Poor 8/9/2004 35 170 5 34 7.65 Poor 7/5/2005 30 138 5 27 7.41 Poor 9/16/2006 39 159 4 22 7.69 Poor 6 11 2008 37 122 1 3 7.35 Poor TFO.OTC 6 11 2008 46 169 2 11 7.35 Poor Qua14 4 8 2010 35 103 3 12 8.01 Poor 4 8 2010 40 129 3 12 7.97 Poor 8 12 2010 45 -- 6 26 7.43 Fair 4/13/2011 35 -- 3 -- 7.74 Poor 5/8/2014 25 70 6 8.13 Poor 4/7/2016 10 22 2 7.00 Poor 3/28/2018 34 95 4 17 7.46 Poor 101 City Hall Plaza, Third Floor, Durham, NC 27701 I 919.560.4326 I DurhamNC.gov I Follow Us @CO o© rhamNC Appendix II- Taxon and Tolerance Values of Collected Macroinvertebrates from the 2018 Sampling Season. Appendix II -Taxon and Tolerance Values of North Carolina Benthic Macroinvertebrates collected by City of Durham contractors in 2018. This list is not exhaustive of all Taxa found in North Carolina. Taxon Tolerance Value Taxon Tolerance Value Taxon Tolerance Value EPHEMEROPTERA DIPTERA; ODONATA CHIRONOMIDAE Family Baetidae Ablabesmyia mallochi 7.4 FamilyAeshnidae Plauditus dubius 2.2 Cladotanytarsus spp 4.0 Boyeria vinosa 5.6 Pseudocloeon propinquum 5.8 Cricotopus bicintus 8.7 Family Calopterygidae Family Caenidae Cricotopus/Orthocladius 3.4 Hetaerina spp 4.9 Caenis spp 6.8 Cricotopus vierriens group 5.4 Family Coenagrionidae Family Heptageniidae Cryptotendipes spp 6.2 Argia spp 8.3 Stenacron interpunctatum 6.4 Dicrotendipes simpsoni 9.8 Enallagma sp 8.5 PLECOPTERA Dicrotendipes modestus 9.4 Family Corduliidae Family Nemouridae Dicrotendipes neomodestus 7.9 Neurocordulia obsoleta 5.3 Amphinemura spp 3.8 Diplocladius cultirger 8.0 Somatochlora spp 8.9 Prostoia spp 5.2 Hydobaenus spp 9.2 Family Gomphidae Family Perlidae Kiefferulus dux Gomphus spp 5.9 Perlesta spp 2.9 Natarsia spp 9.6 Hagenius brevistylus 4.4 TRICHOPTERA Nilotanypus fimbratus 4.1 Stylogomphus albistylus 5.0 Family Hydropsychidae Orthocladius oliveri: 4.4 Family Libellulidae Cheumatopsyche spp 6.6 Orthocladius robacki 6.4 Perithemis spp 9.4 Hydropsyche betteni 7.9 Orthocladius rubicundus 5.7 Family Macromiidae Family Limnephilidae Para. nigrohalteralis 4.9 Macromia spp 6.2 Ironoquia punctatissima 6.7 Phaenopsctra obediens 6.5 CRUSTACEA MISC DIPTERA Polypedilum halterale 7.4 Family Cambaridae Family Simuliidae Polypedilum fallax 6.5 immature crayfish 7.5 Simulium spp 4.9 Polypedilum flavum 5.7 Family Talitridae Family Tipulidae Polypedilum illinoense 8.7 Hyalella spp 7.2 Hexatoma spp 3.5 Polypedilum scalaenum 8.5 ORDER HEMIPTERA Tipula spp 7.5 Polypedilum tritum Metrobates spp OLIGOCHAETA Rheocricotopus robacki 4.7 Ranatra spp 6.3 Family Lumbriculidae 7.0 Rheotanytarsus spp 6.5 Rhagovelia spp Family Tubificidae Stelechomyia perpulchrum 4.0 Rheumatobates spp Ilyodrilus templetoni 9.3 Stenochironomus spp 6.3 Sigarra spp 8.7 Limnodrilus hoffineisteri 9.4 Stictochironomus devinctus 6.3 Synorthocladius spp 4.2 Thienemannimyia group 8.4 Tribelos jacundum 5.7 Tvetenia vitrces 3.5 101 City Hall Plaza, Third Floor, Durham, NC 27701 919.560.4326 DurhamNC.gov Follow Us @CityofDurhamNC itA Appendix II- Taxon and Tolerance Values of Collected Macroinvertebrates from the 2018 Sampling Season. Taxon Tolerance Value Taxon Tolerance Value Taxon Tolerance Value COLEOPTERA MOLLUSCA MEGALOPTERA Family Dryopidae FamilyAncylidae Family Corydalidae Helichus spp 4.1 Ferrissia spp 6.6 Corydalus cornutus 5.2 Family Dytiscidae Family Hydrobiidae Nigronia serricornis 4.6 Neoporus mellitus 3.9 Amnicola spp 4.1 Family Sialidae Family Elmidae Family Physidae Sialis spp 7.0 Dubiraphia vittata 5.0 Physa spp 8.7 OTHER TAXA Macronychus glabratus 4.7 Planorbidae Family Glossihoniidae Microcylloepeus spp 3.3 Helisoma anceps 6.6 Desserobdella phalera 6.6 Tanytarsus gibbus 6.6 Family Pleuroceridae Placobdella ornata Tanytarsus sp 0 6.6 Elimia spp 2.7 Family Planariidae Thienemaniella spp 6.4 Leptoxis spp 1.7 Dugesia tigrina 7.1 Stenelmis spp 5.6 Family Viviparidae Family Pyralidae Family Hydrophilidae Campeloma decisum 5.8 Family Tetrastemmatidae Sperchopsis tessalatus 4.4 Family Corbiculidae Prostoma graecens 6.6 Family Psephenidae Corbicula fluminea 6.6 Family Hydrachnidae Psephenus herricki 2.3 Family Sphaeriidae Hydracarina spp 5.5 101 City Hall Plaza, Third Floor, Durham, NC 27701 1 919.560.4326 1 DurhamNC.gov I Follow Us @CityofDurhamNC Benthic macroinvertebrate monitoring study plan, 2019 Susan Gale, Water Quality Specialist City of Durham, Department of Public Works, Stormwater and GIS Services February 28, 2019 To meet requirements of the City of Durham's National Pollutant Discharge Elimination System (NPDES) Stormwater permit, the City performs ambient water quality monitoring at selected locations throughout the City and County. The ambient monitoring program primarily focuses on monthly water chemistry monitoring but is supplemented by assessment of instream biological communities. For 2019, a total of 6 ambient monitoring sites have been selected for monitoring and assessment of benthic macroinvertebrate communities. In addition to the benthic macroinvertebrate monitoring to be completed as part of the City's ambient monitoring program, specialized data collections will be conducted this year at five locations as part of a special study that will compare indicators of toxicity (midge deformities) to sediment chemistry. Ambient monitoring sites In order to provide greater coverage and characterizations of the surface waters within and immediately downstream of the City's boundary, ambient monitoring is performed on a rotating two-year basis: a certain subset of monitoring sites are sampled in even years, and a second subset are monitored in odd years. The sites to be monitored for benthic macroinvertebrates in 2019 ("odd -year sites") are shown in Table 1Error! Reference source not found. and Figure 1. All monitoring sites and the majority (if not entirety) of their watersheds are located within the Triassic Basins Level IV ecoregion. Previous results from these sites are summarized in Table 2 at the end of this document. Table 1 Benthic macroinvertebrate monitoring locations for calendar year 2019. River basin Watershed Site code Stream Location Latitude (dd.ddd) Longitude (dd.ddd) Sampling method Drainage area (mi.z) Cape Fear New Hope NH2.3MC Mud Cr Pickett Rd 35.9753 -78.9844 Qual4 5.4 NHI.OSC Sandy Cr Garrett Rd 35.9571 -78.9738 Qual4 6.9 Neuse Ellerbe EL1.9EC Ellerbe Cr Glenn Rd 36.0596 -78.8327 Full Scale 21.9 EL5.5GC Goose Cr Camden Ave 36.0248 -78.8609 Qual4 6.4 EL7.1EC Ellerbe Cr Acadia St 36.0200 -78.8952 Qual4 5.7 Panther PN2.4PN Panther Cr Burton Rd 36.0338 -78.8124 Qual4 2.6 There are two significant changes to sampling locations as compared to prior odd -year sites: Site EL5.6EC (Ellerbe Cr. at Midland Terrace) is being discontinued this year. This reach has been monitored regularly from 2006-2017 but it has consistently received a Poor bioclassification with Biotic Index (BI) scores >7.00. It is not anticipated that additional sampling will gain any additional information. There are two other monitoring locations on Ellerbe Cr. (above and below this site) as well as on a major tributary (Goose Cr.), which should provide sufficient coverage for this watershed. Site EL7.9EC (Ellerbe Cr. at Murray Ave.) is being discontinued and replaced by EL7.1EC (Ellerbe Cr. at Acadia St.), the location of a USGS stream gage. This same site change was made to the monthly water chemistry monitoring program in 2018 to enhance the accuracy of chemical constituent loading calculations. Site EL7.1EC has never been sampled by the City for benthic macroinvertebrates, but this is a restored stream reach and appears to have sufficient habitat to support this type of community. Ambient monitoring methods and schedule The sampling method to be used (either Full Scale or the more abbreviated Qua14) at each site is indicated in Table 1. The method was determined in accordance with the City of Durham Stormwater & GIS Services' Standard Operating Procedures (City of Durham, 2019), which are very similar to the methods used by the NC Department of Environmental Quality's (NCDEQ) benthic monitoring program (NCDEQ, 2016). A key difference is the selection of method and calculation of bioclassification and other metrics for streams located in the Triassic Basins ecoregion: the Qua14 sampling method is used for watershed drainage areas up to 20 square miles in this ecoregion. A second key difference is the assignment of bioclassifications for Triassic Basins streams; while NCDEQ does not rate these streams, the City does calculate bioclassifications for streams in the limited area in and near the City. In accordance with the SOP, all Qual4 samples should be collected March 1— May 31. Full Scale samples should be collected June 1—August 31. In addition to completing benthic macroinvertebrate sampling, the following tasks should be completed at each site: instream habitat assessment; completion of a "collection card" site information form; basic field water chemistry measurements (dissolved oxygen, specific conductance, pH, temperature, turbidity); and digital photographs of each location. Benthic macroinvertebrate monitoring services for CY 2019 are being contracted out to Eaton Scientific. The contractor should provide one additional staff member for field sampling as well as necessary field equipment for collection of macroinvertebrate samples and any materials/equipment necessary for laboratory identifications and data analysis. Electronic results (taxonomic list, relative abundances, calculated metrics, and bioclassifications in an Excel spreadsheet), original hard copy field forms, and preserved specimens should be provided to the City of Durham Stormwater & GIS Services by November 30, 2019. The City will provide one staff member to assist with field sampling as well as field meters, field forms, and a digital camera to collect field measurements and photos. Midge toxicity sample collections and analyses The City of Durham will be requesting additional data collections and analyses at five locations within the City to support a special study. These will support midge deformity analyses to determine potential toxicity at each location. Sampling and analysis will be completed by Eaton Scientific in accordance with the NC Department of Environmental Quality benthic monitoring SOP (NCDEQ, 2016). The Quality Assurance Project Plan (QAPP) is currently being developed for this study, and will be provided to Eaton Scientific for review and comment. It is anticipated that sampling for this special study will occur in the spring (April — May 2019), but will be scheduled with input from Eaton Scientific. References City of Durham, Department of Public Works, Stormwater & GIS Services. 2019. Standard Operating Procedures for Benthic Macroinvertebrate Community Monitoring, DRAFT, v. 0.0. Water Quality Unit. Durham, NC. NC Department of Environmental Quality (NCDEQ). 2016. Standard Operating Procedures for the Collection and Analysis of Benthic Macroinvertebrates. Division of Water Resources. Raleigh, North Carolina. 0 IE P Contents L Vpr Est) BenthicSftes2019 F—r i M Qua14 e? IV FuIlScale Heb- d ID ELI 9F i� Coj USGS National Hydrography Dataset (N H D y Cached ► _j U.S. Level III and IV ECOTeglOnS (EPA) Q, 7 . :==7' k % PIN PN_,�F__, E ar, Craas -ib Z� ► Streets aW", o EL7 1frEC h7 "o _j W bab D U Uii' rSky C. E4 N 'SM C H2 E L.iw, 2T 4 cq . . . . . . . . . IN l7. -SC L -7 J k" J/ N F 111�1;7_ k Chapel-Hifl 7 154i! Em_= AmGIS P&rketpla _ 14EIP •j- %gi Te of Use Pn cy Contact Ean 0 K-mrtAb� • Carolina DOT E-n HERE. Gi, NGA USGS -N PS I a P service:.. J _ I 1 Overview map of City of Durham benthic m acroi nverteb rate ambient monitoring sites for calendar year 2019. Interactive map available online at http://arcg.is/1o8zT1 Table 2 Results from previous benthic monitoring (2006-2017) at sites being sampled in 2019, where: 5 = total # taxa; N = total # individuals; EPT 5 = total # EPT taxa; EPT N = total # EPT individuals; BI = biotic index; WQ rating = final bioclassification Date S N rating 8/30/2006 29 101 6 17 7.35 Fair 3/19/2008 26 74 5 16 8.27 Poor 3/19/2008 30 109 5 - 7.74 Poor 2/12/2009 51 204 5 25 7.61 Poor 9/16/2009 37 106 7 45 6.89 Fair EL1.9EC 9/16/2009 48 158 7 48 7.18 Fair 7/1/2010 64 - 8 32 6.87 Fair 6/17/2011 45 - 6 26 6.91 Fair 6/13/2013 28 - 3 - 7.3 Poor 2015 29 90 6 - 5.92 Good/Fair 7/17/2017 56 240 12 - 6.85 Fair 10/3/2006 31 104 3 16 7.85 Poor 3/19/2008 13 30 0 0 8.52 Poor 3/19/2008 18 83 0 0 8.44 Poor 2/11/2009 22 79 2 11 7.55 Poor EL5.5GC 2/11/2009 29 125 2 20 7.82 Poor 4/8/2011 36 - 3 - 7.72 Poor 4/26/2013 35 - 3 - 7.9 Poor 4/8/2015 16 90 3 - 7.01 Poor 3/30/2017 42 154 3 - 7.28 Poor EL7.1EC No prior data available 9/18/2006 29 104 3 12 7.29 Fair 7/9/2007 30 98 5 22 7.09 Poor 3/10/2009 35 137 4 13 7.62 Poor 3/10/2009 43 192 5 17 7.62 Poor NHI.OSC 4/29/2010 53 235 4 24 7.75 Poor 8/16/2010 49 - 6 21 7.61 Poor 4/14/2011 23 - 4 - 7.52 Poor 4/25/2013 48 - 5 - 7.4 Poor 4/7/2015 16 55 2 - 6.85 Fair 3/30/2017 41 95 4 - 7.27 Poor 3/9/2009 55 207 6 24 7.61 Poor 4/28/2010 59 215 9 51 6.35 Fair NH2.3MC 4/15/2011 28 - 10 32 6.34 Fair 4/25/2013 53 - 7 - 6.8 Fair 4/7/2015 26 75 5 - 5.17* Good* 3/30/2017 64 194 12 - 6.61 Good -Fair 3/18/2008 26 101 5 16 7.8 Poor 2/9/2009 39 169 8 31 7.01 Fair 5/4/2010 50 145 5 29 7.41 Poor PN2.4PN 3/21/2011 28 - 3 - 7.64 Poor 5/16/2013 41 - 3 - 6.3 Fair 4/7/2015 29 77 6 - 5.72 G/F 3/30/2017 41 121 6 - 5.88 Fair * BI and WQ rating shown are those reported by contract biologist. Due to anomalous nature of results, BI was recalculated using raw data. Recalculated 131 was 5.54, which is a WQ rating of G/F Benthic macroinvertebrate monitoring study plan, 2018 Susan Gale, Water Quality Specialist City of Durham, Stormwater and GIS Services March 2, 2018 To meet requirements of the City of Durham's National Pollutant Discharge Elimination System (NPDES) Stormwater permit, the City performs ambient water quality monitoring at selected locations throughout the City and County. While the ambient monitoring program primarily focuses on monthly water chemistry monitoring, it is supplemented by benthic macroinvertebrate community monitoring. For 2018, a total of 7 ambient monitoring sites have been selected for benthic macro invertebrate monitoring. In addition to the benthic macroinvertebrate monitoring to be completed as part of the City's ambient monitoring program, additional specialized data collections will be conducted as part of a special study (Sandy Creek Watershed Study, project #16-001). The additional monitoring will support midge deformity analyses at four locations in the watershed; this information will be used as an independent assessment of potential toxicity to aquatic invertebrates at these locations. Site information Details on monitoring locations are provided in the appendices. Information is grouped by program/project: ambient monitoring is included in Appendix 1 and the Sandy Creek Watershed special study is in Appendix 2. Schedule and responsibilities Benthic macroinvertebrate monitoring services for calendar years 2017-2018 are being contracted out to Eaton Scientific, LS. The contractor should provide one additional staff member for field sampling for ambient monitoring sites. The contractor will determine additional staff needs for field sampling associated with the midge deformity analyses being completed as part of the Sandy Creek Watershed special study. For ambient monitoring sampling, City of Durham Stormwater & GIS Services will be able to provide one staff member to assist with field sampling, though they may have little or no experience with benthic macroinvertebrate sampling. The City will also provide field meters, field forms, and a digital camera for these sampling events. Eaton Scientific should provide all other necessary field equipment for all sampling (ambient and special study sites) and any materials/equipment necessary for laboratory identifications and data analysis. In accordance with the SOP, all Qua14 samples collected for the ambient monitoring program should be completed between March 1— May 31. Full Scale samples completed as part of the ambient monitoring program must be completed between June 1— August 31. Sampling for the midge deformity analyses will be completed by the contractor without assistance from COD SW staff. Sampling should occur between August 1— September 30, as specified in the schedule provided in the study's Quality Assurance Project Plan. Field water chemistry measurements, habitat assessments, and photographs do not need to be completed during these field visits. All results should be reported to the COD SW project manager as specified in the current contract. References City of Durham, Stormwater & GIS Services (COD SW). 2017. Standard Operating Procedures for Benthic Macroinvertebrate Community Monitoring for Determination of Bioclassifications, DRAFT, v. 0.0. Water Quality Unit. Durham, NC. City of Durham, Stormwater & GIS Services. 2018. Sandy Creek Watershed Study (Project 316-001) Quality Assurance Project Plan. Water Quality Unit. Durham, NC. NC Department of Environmental Quality (NCDEQ). 2016. Standard Operating Procedures for the Collection and Analysis of Benthic Macroinvertebrates. Division of Water Resources. Raleigh, North Carolina. Appendix 1: Ambient monitoring sampling information In order to provide greater coverage and characterizations of the surface waters within and immediately downstream of the City's boundary, all ambient monitoring is performed on a rotating two-year basis: a certain subset of the monitoring sites are sampled in even years, and a second subset are monitored in odd years. The sites to be monitored for benthic macroinvertebrates in 2018 ("even year sites") are shown in Table 1 (below). A map of locations is provided in Figure 1 at the end of this document and an online interactive map is available at http://arcg.is/lnlaHu. Site information sheets will be provided in a separate document. Previous results from these sites are summarized in Table 2. Table 1 Benthic macroinvertebrate ambient monitoring locations for calendar year 2017. River Lat/Long Drainage Sampling basin Watershed Site code Stream Location (dec. deg.) Ecoregion area (mi.2) method 35.9226 Triassic Third Fork TFO.OTC Third Fork Cr. NC 751 16.1 Qua14 a -78.9524 Basins LL Northeast NEO.ONE Northeast Cr. Sedwick Rd. 35.8868 Triassic 12.9 Qual4 -78.8998 Basins Johnson Mill 35.1416 Little LR9.6LR Little R. Slate Belt 78.2 Full Scale Rd. -78.9208 West Point on 36.0716 EN8.9ER Eno R. Slate Belt 141.1 Full Scale the Eno park -78.9100 a, Eno Penny's Bend 36.0731 a, EN4.9ER Eno R. Slate Belt 149.3 Full Scale z access -78.8635 Mineral Springs 35.9850 Triassic L. Lick LL3.4LLC Little Lick Cr. 6.0 Qual4 Rd. -78.8198 Basins 35.9777 Triassic Lick LC1.1LC Lick Cr. Southview Rd. 10.8 Qua14 -78.7496 Basins Comments about this year's sampling locations: In previous years, Third Fork Cr. was monitored at NC751 (site TFO.OTC) to capture conditions at the watershed outlet. In January 2018, this monthly water chemistry monitoring location was permanently moved to Woodcroft Rd. (site TFI.OTC) to coincide with the location of the USGS stream gage. However, the new location is not suitable for benthic macroinvertebrate sampling due to a lack of appropriate habitat and poor stream flow conditions. Benthic macroinvertebrate monitoring for the Third Fork watershed will still occur at the NC751 location (TFO.OTC) and will be re -assessed prior to 2020 monitoring. Monthly water chemistry monitoring by the City has been temporarily suspended this year at the site on the Eno R. at Penny's Bend access (site EN4.9ER) since a cooperating agency (Upper Neuse River Basin Association) is performing water chemistry monitoring this location as part of a special study. Benthic macroinvertebrate monitoring will be completed by the City, since the UNRBA is not collecting biological community data. The sampling method to be used (either Full Scale or Qual4) at each site is determined by the City of Durham Stormwater & GIS Services' Standard Operating Procedures (COD SW 2017), which are very similar to the methods used by the NC Department of Environmental Quality's benthic monitoring program. A key difference is the selection of method and calculation of bioclassification and other metrics for streams located in the Triassic Basins ecoregion: the Qua14 sampling method is used for watershed drainage areas up to 20 square miles in this ecoregion. The second key difference is the assignment of bioclassifications for Triassic Basins streams; while NC DEQ does not rate these streams, the COD SW does calculate bioclassifications for the streams in the limited area in and near the City. In addition to completing benthic macro invertebrate sampling, the contract biologist is responsible for ensuring that the following are completed for each site: instream habitat assessment; "collection card" form; basic field water chemistry measurements (dissolved oxygen, specific conductance, pH, temperature, turbidity); and digital photographs of sampling location. r olp El S.i . 6.3 nli4 Legend r'f Durham Stormwater — Benthic !!! Macroinvertebrate sites a.r.. 2018 f Qua14 n � t { Full ScaleENS L a�lrlf 7yt f _ ,. LY } '% �F#1_ G � �I ' � 5r , '•. : ; L. i.,, � err '1 � - t`sI- � r Esri.com Help Terms of Use Privacy Contact Esri Report .�, 41i71 Abuse i L ar y To,,n of Cary, State Df Figure 1 Overview map of City of Durham benthic macroinvertebrate ambient monitoring sites for calendar year 2017. Interactive map available online at http://arcg.is/1nIaHu. Table 2 Results from previous benthic monitoring (2002-2016) at sites being sampled in 2018, where: S = total # taxa; N = total # individuals; EPT S = total # EPT taxa; EPT N = total # EPT individuals; BI = biotic index; WQ rating = final bioclassification. Samples with the WQ Rating was missing or "Not Rated" have been excluded. Site ID Collection Date S N EPT S EPT N 131 WQ rating 8/6/2002 34 174 17 95 5.46 Good 8/28/2003 48 218 15 122 5.89 Good -Fair 6/23/2004 52 -- 14 87 5.82 Good -Fair 6/17/2005 43 204 15 95 5.72 Good - Fair 6/21/2006 63 211 18 90 6.50 Good -Fair 6/29/2007 53 195 20 99 5.76 Good -Fair EN4.9ER 6/9/2008 57 237 19 110 5.94 Good -Fair 6/23/2009 51 261 17 147 5.86 Good -Fair 6/29/2010 51 212 23 146 5.37 Good 6/16/2011 81 -- 27 139 5.96 Good 7/19/2012 56 -- 20 111 6.08 Good -Fair 8/7/2014 46 177 22 -- 5.28 Good 7/14/2016 49 175 23 -- 4.94 Good 5/30/2002 55 265 21 116 5.30 Good 6/24/2003 43 190 12 65 6.24 Good -Fair 6/22/2004 44 181 18 100 5.22 Good 6/13/2005 56 -- 19 115 5.20 Good 6/20/2006 50 195 18 78 5.87 Good -Fair EN8.9ER 6/19/2007 69 253 30 123 5.72 Good 6/10/2008 55 193 21 82 6.03 Good -Fair 6/24/2009 62 252 18 100 5.70 Good -Fair 6/28/2010 59 219 18 92 5.92 Good -Fair 6/14/2011 63 -- 17 80 5.97 Good -Fair 8/7/2014 51 254 20 -- 5.10 Good 6/23/2016 45 164 20 -- 5.26 Good 7/15/2004 46 -- 7 24 7.09 Fair 6/16/2005 27 112 6 14 7.43 Fair 8/15/2006 37 131 10 48 6.67 Fair 7/9/2007 22 41 4 10 7.15 Poor 3/14/2008 23 96 6 28 7.35 Fair LC1.1LC 6/30/2009 56 233 8 -- 7.06 Fair 4/20/2010 44 163 8 66 6.11 Fair 7/2/2010 71 -- 7 40 7.18 Fair 5/9/2012 52 -- 9 35 6.97 Fair 5/8/2014 21 60 6 -- 6.35 Fair 4/7/2016 29 91 10 -- 5.62 Fair Site ID Collection Date S N EPT S EPT N 131 WQ rating 8/1/2002 20 97 2 20 8.07 Poor 9/3/2003 31 133 4 13 8.02 Poor 7/22/2004 29 110 1 10 7.29 Poor 6/15/2005 23 142 1 10 7.46 Poor 8/24/2006 38 136 2 4 8.35 Poor 6/12/2008 41 135 2 13 8.25 Poor LL3.4LLC 6/12/2008 32 92 2 4 8.26 Poor 2/16/2009 41 133 4 8 7.96 Poor 2/16/2009 35 108 4 8 7.77 Poor 4/13/2011 35 -- 3 -- 7.99 Poor 5/9/2012 52 -- 5 25 7.62 Poor 5/8/2014 29 63 3 3 7.22 Poor 4/7/2016 18 44 3 -- 6.03 Poor 8/25/2004 77 -- 29 168 5.33 Good 6/20/2005 63 315 29 155 5.09 Excellent 8/2/2006 86 320 24 141 5.6 Good 8/21/2007 80 342 18 107 5.84 Good -Fair LR9.6LR 6/17/2008 72 257 24 120 5.59 Good 7/9/2009 66 314 25 152 5.76 Good 8/19/2010 62 -- 20 138 5.45 Good -Fair 8/15/2012 78 -- 27 161 5.58 Good 8/6/2014 68 254 27 -- 4.91 Good 6/23/2016 50 217 25 -- 4.91 Good 6/25/2002 43 166 9 34 7.55 Poor 7/15/2003 37 140 5 34 7.16 Poor 8/10/2004 33 -- 5 23 7.57 Poor NEO.ONE 7/11/2005 38 190 4 33 7.57 Poor 9/21/2006 34 130 3 9 7.92 Poor 8/2/2007 36 -- 3 -- 7.85 Poor 5/8/2014 18 43 2 -- 7.89 Poor 4/7/2016 19 69 2 -- 7.03 Poor 8/14/2002 37 190 3 14 8.08 Poor 9/11/2003 34 157 4 24 7.26 Poor 8/9/2004 35 170 5 34 7.65 Poor 7/5/2005 30 138 5 27 7.41 Poor 9/16/2006 39 159 4 22 7.69 Poor 6/11/2008 37 122 1 3 7.35 Poor TFO.OTC 6/11/2008 46 169 2 11 7.35 Poor 4/8/2010 35 103 3 12 8.01 Poor 4/8/2010 40 129 3 12 7.97 Poor 8/12/2010 45 -- 6 26 7.43 Fair 4/13/2011 35 -- 3 -- 7.74 Poor 5/8/2014 25 70 6 -- 8.13 Poor 4/7/2016 10 22 2 -- 7.00 Poor Appendix 2: Sandy Creek Watershed Study midge deformity sampling information The City of Durham is requesting additional field collections at four locations to support the Sandy Creek Watershed Study. The additional collections will support midge deformity analyses to determine potential toxicity at these locations. This analysis is above and beyond the standard community monitoring described above, and will be completed in accordance with the NC Department of Environmental Quality benthic monitoring SOP (NCDEQ, 2016). Additional requirements for field data collections, staff, and cost have been discussed with Eaton Scientific, and will require additional sampling events to collect the number of Chironomus specimens at each site that are required to complete this analysis (approximate minimum of 20/site). Monitoring locations are described in Table 2. A map showing locations of sites is attached as Figure #. An online interactive Google Map of all sites in the special study is available at https://drive.google.com/open?id=ldOlmLTyzvclR8VPMiFCibN911RQPw3Vo&usp=sharing. Table 3 Midge deformity sampling sites for Sandy Creek Watershed special study Lat/Long Site ID Stream Location (dec degrees) Comment NH4.7SC Sandy Cr. Morreene Rd. 36.0035 Headwaters; Duke campus -78.9522 NH1.6SC Sandy Cr. Larchmont Rd. 35.9647 Outlet of Sandy Cr. study area (Chapel Hill Rd. service road) -78.9695 NH4.4SCTD Tributary D Academy Rd. 35.9908 Headwaters -78.9400 NH1.8SCTA Tributary A Martin Luther King Pkwy. 35.9614 Majority of watershed piped; -78.9608 very high % impervious area ��11�■Nrr r;0e11rr�� 1 ��■ .IIIoil i��rrrr�- � �; �M� Ilrlllrrlli:� M Now t �` �►� '� it OEM. I t YO&I r� 110, AN r ■ Core site — Streams Studv area — Durham streams ?" Figure 2 Overview map of locations for midge deformity monitoring sites for the Sandy Creek watershed study. An online interactive map is also available at https://drive.google.com/open?id=ldOlmLTyzvclR8VPMiFCibN911RQPw3Vl&usp=sharing.