HomeMy WebLinkAboutNC0020451_Renewal (Application)_20211103 -y-vd
ROY COOPER I� '
Governor
ELIZABETH S.BISER
Secretary
S.DANIEL SMITH NORTH CAROLINA
Director Environmental Quality
November 05, 2021
Town of West Jefferson
Attn: Brandon Patrick, Town Manager
PO Box 490
West Jefferson, NC 28694-0490
Subject: Permit Renewal
Application No. NC0020451
West Jefferson WWTP
Ashe County
Dear Applicant:
The Water Quality Permitting Section acknowledges the November 3, 2021 receipt of your permit renewal application
and supporting documentation. Your application will be assigned to a permit writer within the Section's NPDES WW
permitting branch. Per G.S. 150B-3 your current permit does not expire until permit decision on the application is made.
Continuation of the current permit is contingent on timely and sufficient application for renewal of the current permit. The
permit writer will contact you if additional information is required to complete your permit renewal. Please respond in a
timely manner to requests for additional information necessary to allow a complete review of the application and renewal
of the permit.
Information regarding the status of your renewal application can be found online using the Department of Environmental
Quality's Environmental Application Tracker at:
https://deu.nc.gov/permits-regulations/permit-guidance/environmental-application-tracker
If you have any additional questions about the permit, please contact the primary reviewer of the application using the
links available within the Application Tracker.
Sincerely,.174.-441
Wren Thedford
Administrative Assistant
Water Quality Permitting Section
ec: WQPS Laserfiche File w/application
•
North Carolina Department of Environmental Quality I Division of Water Resources
Wmston-Sakm Regional Office 1450 West Hanes MIII Road,Suite 300 Winston-Salem.North Carolina 27105
**”.9-.NSk9-.NSk,...c.'' 336.776.9800
,
Tom Hartman,Mayor F 'tz Russell W. Barr, Ill,Alderman
Brantley Price,Town Manager vt,;+ - % Calvin Green,Alderman
Bradley Jordan,Chief of Police / Crystal C. Miller,Alderman
John B."Jak"Reeves,Town Attorney John K. Reeves,Alderman
4'rv, Stephen Shoemaker,Alderman
TO' I Of est A
erson
Established 1915
"Prosperity, growth,Achievement"
10/28/2021
NCDEQ/Division of Water Resources RECEIVED
Complex NPDES Permitting Unit NOV 0 3 2021
1617 Mail Service Center
Raleigh, NC, 27699-1617 NCDEQ/DWR/NPDES
Please find enclosed our request for renewal of Permit No. 0020451 for the Town of West
Jefferson.
Let me know if you need further information or have any questions.
Sincerely,
Brandon Patrick
Town of West Jefferson
3362463558
3369776725
wwtp@townofwj.com
Post Office Box 490 of S.Jefferson Ave. West Jefferson, NC 28694
Phone:336-246-3551 Fax:336-246-4409
This institution is an equal opportunity provider and employer.
www.townofwjnc.com
Form U.S. Environmental Protection Agency
2A ;.EPA Application for NPDES Permit to Discharge Wastewater
NPDES NEW AND EXISTING PUBLICLY OWNED TREATMENT WORKS
SECTION 1. BASIC APPLICATION INFORMATION FOR ALL APPLICANTS (40 CFR 122.21(j)(1) and (9))
Tc 1.1 Facility name
E Town of West Jefferson 1
04
_- Mailing address(street or P.O. box)
,e PO Box 490
5 City or town State ZIP code
to
LL' West Jefferson NC 28694
Contact name (first and last) Title Phone number Email address
1 Brandon Patrick ORC 336-246-3558 wwtp@townofwj.com
Location address(street, route number, or other specific identifier) El Same as mailing address:
335 Clearwater Drive
City or town State ZIP code
West Jefferson NC 28694
1.2 Is this application for a facility that has yet to comme a discharge?
ElYes 3 See instructions on data submission No
requirements for new dischargers.
7 1.3 Is pplicant different from entity listed under Item 1.1 above?
E
Yes El No 4 SKIP to Item 1.4.
4 Applicant name
z
as Town of West Jefferson
a
a Applicant address (street or P.O. box)
a PO Box 490
City or town State ZIP code
West Jefferson NC 28694
Contact name (first and last) Title Phone number Email address
Brantley Price Town Manager 336-246-3551 admin@townofwj.com
1.4 Is the applicant the facility's owner, operator, or both? (Check only one response.)
Owner ❑ Operator ❑ Both
1.5 To which entity should the NPDES permitting authority send correspondence? (Check only one
response.)
Facility ❑ Applicant ❑ (Facility and applicant they are one and the same)
tge 1.6 Indicate below any existing environmental permits. (Check all that apply and print or type the
corresponding permit number for each.)
a Existing Environmental Permits
E INPDES (discharges to surface ❑ RCRA(hazardous waste) ❑ UIC (underground
p water) injection control)
.... �eO;G:!o s1
c
lb ❑ PSD (air emissions) ❑ Nonattainment program El NESHAPs (CAA)
c
(CAA)
w
EPA Form 3510-2A(Revised 3-19) Page 33
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
NC0020451 Town of West Jefferson OMB No.2040-0004
❑ Ocean dumping (MPRSA) El Dredge or fill (CWA 0 Other(specify)
Section
404)
6 1.7 Provide the collection system information requested below for the treatment works.
CD
Municipality Population Collection System Type Ownership Status
Served Served (indicate percentage) 1 /
3 Town of West 755 _100_%separate sanitary sewer NJ Own IS
aJefferson %combined storm and sanitary Maintain
sewer 0 Own ❑
❑ Unknown Maintain
tn ❑ Own ❑
to Maintain
c %separate sanitary sewer ❑ Own 0
ati %combined storm and sanitary Maintain
sewer 0 Own ❑
0 0 Unknown Maintain
❑ Own ❑
Maintain
%separate sanitary sewer ❑ Own 0
%combined storm and sanitary Maintain
sewer 0 Own ❑
❑ Unknown Maintain
❑ Own ❑
Maintain
separate sanitary sewer 0 Own 0
%combined storm and sanitary Maintain
sewer 0 Own ❑
❑ Unknown Maintain
❑ Own 0
Maintain
Total
Population 1305
Served
Separate Sanitary Sewer Combined Storm and
System Sanitary Sewer
Total percentage of each type of 100%
sewer line (in miles)
1.8 Is the treatment works located in Indian Country?
0
0 ❑ Yes No
c,,
to 1.9 Does the facility discharge to a receiving water that fl ws through Indian Country?
:a ,
c
❑ Yes No
2 1.10 Provide design and actual flow rates in the designated spaces. Design Flow Rate
g �, 1.O mgd
c ig Annual Average Flow Rates(Actual)
•y LL Two Years Ago Last Year This Year
as
G 0.320 mgd 0.347 mgd 0.288 mgd
Maximum Daily Flow Rates(Actual)
EPA Form 3510-2A(Revised 3-19) Page 34
Two Years Ago Last Year This Year
1.293 mgd 1.528 mgd 0.680 mgd
0 1.11 Provide the total number of effluent discharge points to waters of the United States by type.
a_ Total Number of Effluent Discharge Points by Type
Constructed
a ~ Combined Sewer
Treated Effluent Untreated Effluent Bypasses Emergency
Overflows Overflows
A 1
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
OMB No.2040-0004
Outfalls Other Than to Waters of the United States
1.12 Does the POTW discharge wastewater to basins, ponds, or other surface impoundments that do
not have outlets for discharge to waters of the United States?
Q. ❑ Yes �Q[No 4 SKIP to Item 1.14.
rn
i5 1.13 Provide the location of each surface impoundment and associated discharge information in the
at
table below.
Surface Impoundment Location and Discharge Data
Average Daily Volume Continuous or Intermittent
Q Location Discharged to Surface (check one)
Impoundment
2 ❑ Continuous
U) gpd ❑ Intermittent
0 Continuous
gpd ❑ Intermittent
❑ Continuous
gpd ❑ Intermittent
1.14 Is wastewater applied to land?
❑ Yes riNo 3 SKIP to Item 1.16.
1.15 Provide the land application site and discharge data requested below.
Land Application Site and Discharge Data
Average Daily Volume Continuous or
Location Size Applied Intermittent
(check one)
❑ Continuous
acres gpd ❑ Intermittent
acres gpd 0 Continuous
❑ Intermittent
0 Continuous
acres gpd ❑ Intermittent
1.16 Is effluent transported to another facility for tr atment prior to discharge?
❑ Yes No 4 SKIP to Item 1.21.
EPA Form 3510-2A(Revised 3-19) Page 35
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
NC0020451 Town of West Jefferson OMB No.2040-0004
1.17 Describe the means by which the effluent is transported (e.g., tank truck, pipe).
1.18 Is the effluent transported by a party other than the applicant?
El Yes ❑ No 4 SKIP to Item 1.20.
1.19 Provide information on the transporter below.
Transporter Data
Entity name Mailing address (street or P.O. box)
City or town State ZIP code
Contact name (first and last) Title
Phone number Email address
1.20 In the table below, indicate the name, address, contact information, NPDES number, and average
daily flow rate of the receiving facility.
0 Receiving Facility Data
4.4
o Facility name Mailing address (street or P.O. box)
t
City or town State ZIP code
0
° Contact name (first and last) Title
Phone number Email address
L
NPDES number of receiving facility(if any) ❑
None Average daily flow rate mgd
= 1.21 Is the wastewater disposed of in a manner other than those already mentioned in Items 1.14
through 1.21 that do not have outlets to waters of the United States (e.g., underground percolation,
ca underground injection)?
to
❑ YesNo -) SKIP toIt m 1.2
3.
O 1.22 Provide information in the table below on these other disposal methods.
Information on Other Disposal Methods
Disposal Annual
Method Location of Size of Average Continuous or
Description Disposal Site Disposal Site Daily Intermittent (check one)
Discharge
Volume
acres gpd ❑ Continuous
0 Intermittent
acres gpd ❑ Continuous
0 Intermittent
acres gpd ❑ Continuous
0 Intermittent
EPA Form 3510-2A(Revised 3-19) Page 36
c m 1.23 Do you intend to request or renew one or more of the variances authorized at 40 CFR 122.21(n)?
(Check all that apply.
S Consult with your NPDES permitting authority to determine what information needs to be submitted
and when.)
❑ Discharges into marine waters (CWA Section 301(h)) 0 302(b)(2))Water quality
related effluent limitation (CWA Section
Not applicable
1.24 Are any operational or maintenance aspects (related to wastewater treatment and effluent quality)
of the treatment works the responsibility of a ontractor?
0 Yes No 4SKIP to Section 2.
6 1.25 Provide location and contact information for each contractor in addition to a description of the
contractors operational and maintenance responsibilities.
Contractor Information
t�
-
Contractor 1 Contractor 2 Contractor 3
Contractor name
(company name)
Mailing address
(street or P.O. box)
City, state, and ZIP
code
Contact name (first
and last)
Phone number
Email address
Operational and
maintenance
responsibilities of
contractor
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
NC0020451 Town of West Jefferosn OMB No.2040-0004
SECTION 2.ADDITIONAL INFORMATION(40 CFR 122.21(j)(1)and(2))
Outfalls to Waters of the United States
c 2.1 Does the treatment works have a design flow greater than or equal to 0.1 mgd?
a)
g Yes ❑ No + SKIP to Section 3.
2.2 Provide the treatment works' current average daily Average Daily Volume of Inflow and
volume of inflow and infiltration. Infiltration
60,000gpd
c
co Indicate the steps the facility is taking to minimize inflow and infiltration.
Last year, we videoed the majority of our terracotta lines, and patched 3 holes. We also applied for
a CDBG grant to redo our main sewer lines, all terracotta, in the downtown area. These are our
oldest lines. We have budgeted $200,000 for lining terracotta lines this year.
EPA Form 3510-2A(Revised 3-19) Page 37
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
NC0020451 Town of West Jefferson OMB No.2040-0004
a 2.3 Have you attached a topographic map to this application that contains all the required information?
a. (See instructions for specific requirements.)
og
o.
F°— Yes ❑ No
0 2.4 Have you attached a process flow diagram or schematic to this application that contains all the
required information?
ets
(See instructions for specific requirements.)
Lam' Yes 0 No
2.5 Are improvements to the facility scheduled?
0 Yes No 4 SKIP to Section 3.
E
a)
Q Briefly list and describe the scheduled improvements.
E
1.
v
2.
3.
E
d
o 4.
a.
2.6 Provide scheduled or actual dates of completion for improvements.
d Scheduled or Actual Dates of Completion for Improvements
Affected Attainment of
Scheduled Begin End Begin
,= Outfalls Operational
Improvement Construction Construction Discharge
(from above) (list outfall (MM/DD/YYYY) (MM/DD/YYYY) (MM/DD/YYYY) Level
number) (MM/DD/YYYY)
1.
2.
3.
4.
2.7 Have appropriate permits/clearances concerning other federal/state requirements been obtained?
Briefly explain your response.
❑ Yes ❑ No 0 None required or applicable
Explanation:
SECTION 3. INFORMATION ON EFFLUENT DISCHARGES(40 CFR 122.21(j)(3) to(5))
3.1 Provide the following information for each outfall. (Attach additional sheets if you have more than
three outfalls.)
Outfall Number Outfall Number
O 001 Outfall Number
State NC
U
N
p County Ashe
EPA Form 3510-2A(Revised 3-19) Page 38
City or town West Jefferson
Distance from shore 0 ft. ft. ft.
Depth below surface 0 ft. ft. ft.
Average daily flow 0.322 mgd mgd mgd
rate
Latitude 36°24 ' 20 "
Longitude 80 ° 29' 24 " "
3.2 Do any of the outfalls described under Item 3.1 have sea nal or periodic discharges?
rn
❑ Yes No 4 SKIP to Item 3.4.
y 3.3 If so, provide the following information for each applicable outfall.
0
Outfall Number Outfall Number Outfall Number
o _001
Number of times per
a year discharge occurs
Average duration of
each discharge
co (specify units)
Average flow of each
discharge mgd mgd mgd
Months in which
discharge occurs
48- 3.4 Are any of the outfalls listed under Item 3.1 equipped wit a diffuser?
❑ Yes No 4 SKIP to Item 3.6.
4 3.5 Briefly describe the diffuser type at each applicable outfall.
0
Outfall Number Outfall Number Outfall Number
u uj Does the treatment works discharge or plan to discharge wastewater to waters of the United States
3.6 fro one or more discharge points?
cam
Yes ❑ No +SKIP to Section 6.
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
OMB No.2040-0004
3.7 Provide the receiving water and related information (if known)for each outfall.
Outfall Number Outfall Number
001 Outfall Number
Unknown Tributary
Receiving water name of Little Buffalo
Creek
EPA Form 3510-2A(Revised 3-19) Page 39
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
NC0020451 Town of West Jefferson OMB No.2040-0004
sM Name of watershed, river,
a.
or stream system New River
U
In
U.S. Soil Conservation
9 Service 14-digit watershed 050500001010040
co code
c'`'' Name of state
' management/river basin New River
lY U.S. Geological Survey 8-
digit hydrologic cataloging
unit code
Critical low flow(acute) 0.6 cfs cfs cfs
Critical low flow(chronic) 0.39 cfs cfs cfs
Total hardness at critical mg/L of mg/L of mg/L of
low flow CaCO3 CaCO3 CaCO3
•a
3.8 Provide the following information describing the treatment provided for discharges from each outfall.
'c Outfall Number Outfall Number
us _00I Outfall Number
Q Highest Level of ' Primary ❑ Primary ❑ Primary
E Treatment(check all that 0 Equivalent to ❑ Equivalent to ❑ Equivalent to
m apply per outfall) / secondary secondary secondary
F= Si Secondary 0 Secondary 0 Secondary
0/Advanced 0 Advanced ❑ Advanced
H Other(specify) 0 Other(specify) 0 Other(specify)
Tertiary-Sand Filters
Design Removal Rates
9 by
Outfall
BOD5 or CBOD5 85 % % %
TSS 85 % % %
0 Not applicable 0 Not applicable% ❑ Not applicable
Phosphorus 85 %
0 Not applicable 0 Not applicable % 0 Not applicable%
Nitrogen 85 %
Other(specify) ❑ Not applicable 0 Not applicable% ❑ Not applicable
ck
EPA Form 3510-2A(Revised 3-19) Page 40
EPA NPDES Permit Number Facility Name Form Approved 03/05/19
Identification Number NC0020451 Town of West Jefferson OMB No.2040-0004
3.9 Describe the type of disinfection used for the effluent from each outfall in the table below. If
o disinfection varies by season, describe below.
U
0
0.
U
Outfall Number Outfall Number Outfall Number
_001_
Disinfection type UV
Seasons used All
Dechlorination used? [ Not applicable ❑ Not applicable 0 Not applicable
❑ Yes 0 Yes ❑ Yes
I No 0 No ❑ No
3.10 Have you completed monitoring for all Table A parameters and attached the results to the
application package?
Yes ❑ No
m 3.11 Have you conducted any WET tests during the 4.5 years prior to the date of the application on any
of th facility's discharges or on any receiving water near the discharge points?
w Yes ❑ No -4 SKIP to Item 3.13.
3.12 Indicate the number of acute and chronic WET tests conducted since the last permit reissuance of
the facility's discharges by outfall number or of the receiving water near the discharge points.
Outfall Number Outfall Number Outfall Number
001
Acute Chronic Acute Chronic Acute Chronic
Number of tests of 30
discharge water
Number of tests of
receiving water
3.13 Does the treatment works have a design flow greater than or equal to 0.1 mgd?
11 Yes 0 No 4 SKIP to Item 3.16.
3.14 Does the POTW use chlorine for disinfection, use chlorine elsewhere in the treatment process, or
otherwise have reasonable potential to discharge chlorinein in its effluent?
0 Yes 4 Complete Table B, including chlorine. L10 No 4 Complete Table B, omitting chlorine.
3.15 Have you completed monitoring for all applicable Table B pollutants and attached the results to this
app ication package?
Yes 0 No
EPA Form 3510-2A(Revised 3-19) Page 8
3.16 Does one or more of the following conditions apply?
• The facility has a design flow greater than or equal to 1 mgd.
• The POTW has an approved pretreatment program or is required to develop such a program.
• The NPDES permitting authority has informed the POTW that it must sample for the
parameters in Table C, must sample other additional parameters (Table D), or submit the
ir 'sults of WET tests for acute or chronic toxicity for each of its discharge outfalls (Table E).
Yes 4 Complete Tables C, D, and E as applicable. ❑ No -4 SKIP to Section 4.
3.17 Have you completed monitoring for all applicable Table C pollutants and attached the results to this
app+cation package?
Yes ❑ No
3.18 Have you completed monitoring for all applicable Table D pollutants required by your NPDES
permitting authority and attached the results to this appli tion package?
❑ Yes No additional sampling required by NPDES
permitting authority.
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
NC0020451 Town of West Jefferson OMB No.2040-0004
3 3.19 Has the POTW conducted either(1) minimum of four quarterly WET tests for one year preceding
�__, this permit application or(2)at least four annual WET tests in the past 4.5 years?
.�q_q
Yes ❑ No 4 Complete tests and Item 3.26.
15
Table E and SKIP to
= 3.20 Have you previously submitted the results of the above tests to your NPDES permitting authority?
H
d
a) Yes
0 No 4 Item 3.26. Provide results in Table E
c and SKIP to
W 3.21 Indicate the dates the data were submitted to your NPDES permitting authority and provide a
summary of the results.
Date(s)Submitted Summary of Results
(MM/DD/YYYY)
Report forms are attached from All samples passed.
04/2016 through 07/2021 from two
species.
3.22 Regardless of how you provided your WET testing data t the NPDES permitting authority, did any
of the tests result in toxicity?
❑ Yes No 4 SKIP to Item 3.26.
3.23 Describe the cause(s)of the toxicity:
3.24 Has the treatment works conducted a toxicity reduction evaluation?
❑ Yes ❑ No 4 SKIP to Item 3.26.
EPA Form 3510-2A(Revised 3-19) Page 9
3.25 Provide details of any toxicity reduction evaluations conducted.
3.26 Have you completed Table E for all applicable outfalls and attached the results to the application
package?
❑ Yes [ Not applicable because previously
submitted information to the NPDES permitting authority.
SECTION 4. INDUSTRIAL DISCHARGES AND HAZARDOUS WASTES(40 CFR 122.21(j)(6) and (7))
4.1 Do-s the POTW receive discharges from Sills or NSCIUs?
Ui Yes ❑ No 4 SKIP to Item 4.7.
v° 4.2 Indicate the number of SlUs and NSCIUs that discharge to the POTW.
Number of SIUs Number of NSCIUs
1
a, 4.3 Does the POTW have an approved pretreatment program?
Li'Yes ❑ No
43 4.4 Have you submitted either of the following to the NPDES permitting authority that contains
information substantially identical to that required in Table F: (1)a pretreatment program annual
report submitted within one year of the application or(2)a pretreatment program?
Yes ❑ No 4 SKIP to Item 4.6.
4.5 Identify the title and date of the annual report or pretreatment program referenced in Item 4.4. SKIP
to Item 4.7.
2020 Annual Pretreatment Report submitted on 02/16/2021
4.6 Have you completed and attached Table F to this appli ation package?
❑ Yes No
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
NC0020451 Town of West Jefferson OMB No.2040-0004
4.7 Does the POTW receive, or has it been notified that it will receive, by truck, rail, or dedicated pipe,
any wastes that are regulated as RCRA hazardous w tes pursuant to 40 CFR 261?
❑ Yes No 4 SKIP to Item 4.9.
4.8 If yes, provide the following information:
Annual
Hazardous Waste Transport Method Amount
Waste Number (check all that apply) of Units
Waste
Received
❑ Truck ❑ Rail
❑ Dedicated pipe 0 Other(specify)
EPA Form 3510-2A(Revised 3-19) Page 10
3 ❑ Truck ❑ Rail
❑ Dedicated pipe ❑ Other(specify)
tn
as
❑ Truck 0 Rail
0 Dedicated pipe ❑ Other(specify)
2
f0
4.9 Does the POTW receive, or has it been notified that it will receive, wastewaters that originate from
remedial activities, including those undertaken pursuant to CERCLA and Sections 3004(7)or
3008(h)of RCRA?
U
era to
❑ Yes [VNo 4 SKIP to Section 5.
y4.10 Does the POTW receive (or expect to receive) less than 15 kilograms per month of non-acute
hazardous wastes as specified in 40 CFR 261.30(d)and 261.33(e)?
❑ Yes 4 SKIP to Section 5. ❑ No
4.11 Have you reported the following information in an attachment to this application: identification and
description of the site(s)or facility(ies)at which the wastewater originates; the identities of the
wastewater's hazardous constituents; and the extent of treatment, if any, the wastewater receives
or will receive before entering the POTW?
❑ Yes ❑ No
SECTION 5.COMBINED SEWER OVERFLOWS(40 CFR 122.21(j)(8))
Li 5.1 Does the treatment works have a combined sewer system?
0 Yes No 4 SKIP to Section 6.
k 5.2 Have you attached a CSO system map to this application? (See instructions for map
cis requirements.)
N ❑ Yes ❑ No
5.3 Have you attached a CSO system diagram to this application? (See instructions for diagram
requirements.)
❑ Yes ❑ No
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
NC0020451 Town of West Jefferson OMB No.2040-0004
5.4 For each CSO outfall, provide the following information. (Attach additional sheets as necessary.)
CSO Outfall Number CSO Outfall Number CSO Outfall Number
G
TIs City or town
8 State and ZIP code
cn
County
Latitude '
EPA Form 3510-2A(RevisedPage 11
3-19)
Longitude " ° " ° "
Distance from shore ft. ft. ft.
Depth below surface ft. ft. ft.
•L
5.5 Did the POTW monitor any of the following items in the past year for its CSO outfalls?
o
CSO Outfall Number CSO Outfall Number CSO Outfall Number
0
g
to Rainfall ❑ Yes ❑ No 0 Yes ❑ No 0 Yes 0 No
U
CSO flow volume ❑ Yes 0 No ❑ Yes ❑ No ❑ Yes 0 No
CSO pollutant ❑ Yes ❑ No ❑ Yes ❑ No 0 Yes 0 No
concentrations
Receiving water ❑ Yes 0 No ❑ Yes ❑ No ❑ Yes ❑ No
quality
CSO frequency 0 Yes 0 No ❑ Yes ❑ No ❑ Yes ❑ No
Number of storm ❑ Yes 0 No 0 Yes ❑ No 0 Yes ❑ No
events
a`�i 5.6 Provide the following information for each of your CSO outfalls.
cn CSO Outfall Number CSO Outfall Number CSO Outfall Number
c Number of CSO
wevents in the past events events events
0 year
0 hours hours hours
v Average duration per
event 0 Actual or 0 0 Actual or 0 ❑Actual or ❑
Estimated Estimated Estimated
million gallons million gallons million gallons
Average volume per
event 0 Actual or ❑ 0 Actual or 0 0 Actual or ❑
Estimated Estimated Estimated
Minimum rainfall inches of rainfall inches of rainfall inches of rainfall
causing a CSO event ❑ Actual or ❑ 0 Actual or 0 ❑Actual or 0
in last year Estimated Estimated Estimated
EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
NC0020451 Town of West Jefferson OMB No.2040-0004
m 5.7 Provide the information in the table below for each of your CSO outfalls.
t0 CSO Outfall Number CSO Outfall Number CSO Outfall Number
•� Receiving water
m name
adName of watershed/
v stream system
0 Unknown 0 Unknown 0 Unknown
EPA Form 3510-2A(Revised 3-19) Page 12
U.S. Soil
Conservation
Service 14-digit
watershed code
(if known)
Name of state
management/river
basin
U.S. Geological ❑ Unknown 0 Unknown ❑ Unknown
Survey
8-Digit Hydrologic
Unit
Code (if known)
Description of known
water quality impacts
on receiving stream
by CSO (see
instructions for
examples)
SECTION 6.CHECKLIST AND CERTIFICATION STATEMENT(40 CFR 122.22(a)and(d))
E6.1 In Column 1 below, mark the sections of Form 2A that you have completed and are submitting with
cam, your application. For each section, specify in Column 2 any attachments that you are enclosing to
alert the permitting authority. Note that not all applicants are required to provide attachments.
Column 1 Column 2
Section 1 Information for All
App icants : Basic Application ❑ w/variance request(s) ❑ w/additional attachments
Section 2: Additional Li w/topographic map w/process flow diagram
Information ❑z w/additional attachments
w/Table A ❑ w/Table D
v Section 3: Information on 131
w/Table B ❑ w/Table E
Effluent Discharges
w/Table C Vw/additional attachments
Section 4: Industrial NI w/SIU and NSCIU attachments ❑ w/Table F
Discharges and ❑ w/additional attachments
Hazardous Wastes
❑ Overflows Section 5: ❑ w/CSO map ❑ w/additional attachments
Combined Sewer ❑ w/CSO system diagram
NI Section 6Certification
Statement : Checklist and ❑ w/attachments
Certification Statement
6.2
I certify under penalty of law that this document and all attachments were prepared under my
direction or supervision in accordance with a system designed to assure that qualified personnel
properly gather and evaluate the information submitted. Based on my inquiry of the person or
persons who manage the system, or those persons directly responsible for gathering the
information, the information submitted is, to the best of my knowledge and belief, true, accurate,
and complete. I am aware that there are significant penalties for submitting false information,
including the possibility of fine and imprisonment for knowing violations.
EPA Form 3510-2A(Revised 3-19) Page 13
Name (print or type first and last name) Official title
' Rirk-/-114 1-3)C /Copt
Signature l Date signed
EPA Form 3510-2A(Revised 3-19) Page 14
EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
NC0020451 Town of West Jefferson 001 OMB No.2040-0004
TABLE A.EFFLUENT PARAMETERS FOR ALL POTWS
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of
Value Units Value Units Method` (include units)
Sam_ples ,
Biochemical oxygen demand
❑BOD5 or❑CBOD5 9.06 mg/I 1.57 mg/I 181 SM5210B-2011 ❑ML
(report one) - ❑MDi_
Fecal coliform 517.2 MPN/100m1 25.86 MPN/100m1 183 IDEXX Colilert 1 ❑ML
18MPN MPN/100m1 ❑MDL
Design flow rate 1.528 MGD r 0.322 MGD 1238
i
pH(minimum) 6.14 pH
pH(maximum) 7.62 pH
3r$
Temperature(winter) 22.1 °C 13.5 °C 82
Temperature(summer) 23.1 °C 20.5 °C 84
Total suspended solids(TSS) 19 mg/I 1.213 mg/I 183 SM2540D-2011 2.660 mg/1 0 ML
❑MDL
1 Sampling shall be conducted according to sufficiently sensitive test procedures (i.e., methods)approved under 40 CFR 136 for the analysis of pollutants
or pollutant parameters or required under 40 CFR chapter I, subchapter N or O. See instructions and 40 CFR 122.21(e)(3).
EPA Form 3510-2A(Revised 3-19)
Page 13
This page intentionally left blank.
EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved
NC0020451 Town of West Jefferson 001 03/05/19 OMB No.
2040-0004
TABLE B. EFFLUENT PARAMETERS FOR ALL POTWS WITH A FLOW EQUAL TO OR GREATER THAN 0.1 MGD
Maximum Daily Discharge Average Daily Discharge
Pollutant i Analytical ML or MDL
NumValue Units Value Units mp�es f Method' (include units)
SaAmmonia (as N) 4.48 mg/I 0.12 mg/I 183 ammonia 0.5mg/I ❑ML
0 MDL
Chlorine o ML
(total residual, TRC)2 0 MDL
Dissolved oxygen 10.96 mg/I 7.86 mg/I 181 SM19 4500G 0.1mg/I ❑ML
❑MDL
mg/I mg/I SM19 4500N 0.08mg/I ❑ML
Nitrate/nitrite 5.5 3.18 9 ❑MDL
mg/I mg/I SM19 4500N 0.5mg/I El ML
Kjeldahl nitrogen 7.14 4.59 9 0 MDL
Oil and grease 1.8 mg/I 0.36 mg/I 5 SM19 5520B 1mg/I 0 ML
❑MDL
Phosphorus 4.02 mg/I 1.73 mg/I 11 EPA 365.2 0.02mg/I ❑ML
❑MDL
Total dissolved solids 226 mg/I 147 mg/I 3 SM19 2540C 1mg/I 0 ML
0 MDL
1
1 Sampling shall be conducted according to sufficiently sensitive test procedures (i.e., methods)approved under 40 CFR 136 for the analysis of pollutants
• or pollutant parameters or required under 40 CFR chapter I, subchapter N or O. See instructions and 40 CFR 122.21(e)(3).
2 Facilities that do not use chlorine for disinfection, do not use chlorine elsewhere in the treatment process, and have no reasonable potential to discharge
chlorine in their effluent are not required to report data for chlorine.
EPA Form 3510-2A(Revised 3-19) Page 17
This page intentionally left blank.
EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved
NC0020451 Town of West 001 03/05/19
Jefferson OMB No.2040-0004
TABLE C. EFFLUENT PARAMETERS FOR SELECTED
POTWS
Maximum Daily Discharg. Average Daily Discharge Analytical ML or MDL
Pollutant Number of Method' (include units)
Value Units Value Units Samples
Metals,Cyanide,and Total Phenols
Hardness(as CaCO3) 170 mg/I 79.3 mg/I 17 Calculation ❑ ML
❑ MDL
Antimony, total <0.01 mg/I <0.01 mg/I 3 EPA 200.7 0.01mg/I ❑ ML
recoverable ❑ MDL
Arsenic, total <0.01 mg/I <0.01 mg/I 6 EPA 200.7 0.01mg/I ❑ ML
recoverable ❑ MDL
Beryllium, total <0.001 mg/I <0.001 mg/I 4 EPA 200.7 0.001mg/I 0 ML
recoverable 0 MDL
Cadmium, total <0.0002 mg/I <0.0002 mg/I 5 EPA 200.7 .0001mg/I 0 ML
recoverable ❑MDL
Chromium, total <0.001 mg/I <0.001 mg/I 6 EPA 200.7 0.001mg/I 0 ML
recoverable 0 MDL
Copper, total 0.098 mg/I 0.012 mg/I 45 EPA 200.7 0.001mg/I ❑ML
recoverable 0 MDL
Lead, total recoverable <0.01 mg/I <0.01 mg/I 6 EPA 200.7 0.01mg/I ❑ ML
❑ MDL
Mercury, total 0.687 Ng/I 0.212 Ng/I 6 EPA 245.1 0.0001mg/I ❑ ML
recoverable ❑ MDL
Nickel,total recoverable <0.001 mg/I <0.001 mg/I 6 EPA 200.7 0.001mg/I 0 ML
_ ❑ MDL
Selenium, total 0.298 mg/I 0.016 mg/I 18 EPA 200.7 0.001mg/I ❑ML
recoverable ❑ MDL
Silver,total recoverable <0.001 mg/I <0.001 mg/I 6 EPA 200.7 0.001mg/I ❑ML
0 MDL
Thallium, total <0.001 mg/I <0.001 mg/I 3 EPA 200.7 0.001mg/I ❑ML
recoverable 0 MDL
EPA Form 3510-2A(Revised 3-19) Page 19
Zinc, total recoverable 0.068 mg/I 0.021 mg/I 6 EPA 200.7 0.001mg/I ❑ ML
0 MDL
Cyanide <0.005 mg/I <0.005 mg/I 4 SM19 4500C 0.005mg/I ❑ ML
❑ MDL
Total phenolic <0.01 mg/I <0.01 mg/I 3 EPA 420.1 0.01mg/I 0 ML
compounds ❑ MDL
Volatile Organic Compounds
Acrolein <50 ug/I <50 ug/I 3 EPA 624 50u /1 ❑ ML
g ❑ MDL
Acrylonitrile <50 ug/I <50 ug/1 3 EPA 624 50ug/I ❑ ML
❑ MDL
Benzene <5 ug/I <5 ug/1 3 EPA 624 5 ug/l ❑ ML
0 MDL
Bromoform <5 ug/1 <5 ug/I 3 EPA 624 5 ug/I ❑ ML
0 MDL
EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
NC0020451 Town of West 001 OMB No.2040-0004
Jefferson
TABLE C. EFFLUENT PARAMETERS FOR SELECTED
POTWS
Maximum Daily Discharg: Average Daily Discharge
Pollutant Analytical ML or MDL
Value Units Value Units Number of Method' (include units)
Samples
Carbon tetrachloride <5 ug/I <5 ug/I 3 EPA 624 5 ug/I ❑ ML
❑ MDL
Chlorobenzene <5 ug/I <5 ug/I 3 EPA 624 5 ug/I ❑ML
o MDL
Chlorodibromomethane <5 ugh' <5 ug/I 3 EPA 624 5 ug/I ❑ M
❑MDL
Chloroethane <10 ugh' <10 ugh' 3 EPA 624 10ug/ ❑ ML
❑ MDL
2-chloroethylvinyl ether <10 ug/I <10 ug/I 3 EPA 624 10ug/ ❑ ML
❑ MDL
Chloroform <5 ugh' <5 ug/I 3 EPA 624 5 ug/I ❑M
❑MDL
Dichlorobromomethane <5 ug/I <5 ug/I 3 EPA 624 5 ug/I ❑M
❑MDL
1,1-dichloroethane <5 ug/I <5 ug/I 3 EPA 624 5 ug/I ❑ML
❑ MDL
1,2-dichloroethane <5 ug/I <5 ug/I 3 EPA 624 5 ug/I ❑ ML
❑ MDL
trans-1,2- <5 ug/I <5 ugh' 3 EPA 624 5 ug/I oo ML
MDL
dichloroethylene
1,1-dichloroethylene <5 ug/I <5 ug/I 3 EPA 624 5 ug/I ❑o ML
MDL
1,2-dichloropropane <5 ug/I <5 ug/I 3 EPA 624 5 ug/I ❑ML
o MDL
1,3 dichloropropylene <5 ug/I <5 ug/I 3 EPA 624 5 ug/I ❑ML
o MDL
Ethylbenzene <5 ug/I <5 ug/I 3 EPA 624 5 ug/IJ ❑ML
o MDL
Methyl bromide <10 ug/I <10 ug/I 3 EPA 624 10 ug/l 0 ML
❑ MDL
Methyl chloride <10 ug/I <10 ug/I 3 EPA 624 10 ug/l 0 ML
❑ MDL
EPA Form 3510-2A(Revised 3-19) Page 21
Methylene chloride <5 ug/1 <5 ug/I 3 EPA 624 5 ug/I ❑ ML
❑ MDL
1,1,2,2-tetrachloroethane <5 ug/I <5 ug/I 3 EPA 624 5 u /I ❑ ML
g ❑ MDL
Tetrachloroethylene <5 ug/I <5 ug/1 3 EPA 624 5 ug/I ❑ML
❑MDL
Toluene 21.3 ug/1 7.1 ug/1 3 EPA 624 5 u /I ❑ML
g ❑MDL
1,1,1-trichloroethane <5 ug/1 <5 ug/I 3 EPA 624 5 u /I ❑ML
g ❑MDL
1,1,2-trichloroethane <5 ug/1 <5 ug/I 3 EPA 624 5 u /I ❑ ML
g ❑ MDL
EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved
NC0020451 Town of West 001 03/05/19
Jefferson OMB No.2040-0004
TABLE C. EFFLUENT PARAMETERS FOR SELECTED
POTWS
Maximum Daily Discharg: Average Daily Discharge
Pollutant —__..__ Analytical ML or MDL
Value Units Value Units Number of Method' (include units)
Samples
Trichloroethylene <5 ug/I <5 ug/I 3 EPA 624 5 ug/I ❑ML
❑MDL
Vinyl chloride <2 ug/I <2 ug/I 3 EPA 624 2 ug/1 ❑ML
❑MDL
Acid-Extractable Compounds
p-chloro-m-cresol <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/i 0 ML
❑MDL
2-chlorophenol <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
0 MDL
2,4-dichlorophenol <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑ MDL
2,4-dimethylphenol <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑ MDL
4,6-dinitro-o-cresol <0.07 mg/1 <0.07 mg/1 3 EPA 625 .07mg/I ❑ ML
0 MDL
2,4-dinitrophenol <0.08 mg/I <0.08 mg/1 3 EPA 625 .08mg/I ❑ ML
❑ MDL
EPA Form 3510-2A(Revised 3-19) Page 22
2-nitrophenol <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑MDL
4-nitrophenol <0.04 mg/I <0.04 mg/I 3 EPA 625 .04mg/I 0 ML
❑ MDL
Pentachlorophenol <0.025 mg/I <0.025 mg/I 3 EPA 625 .025mg/I 0 ML
❑ MDL
Phenol <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑MDL
2,4,6-trichlorophenol <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ ML
❑ MDL
Base-Neutral Compounds
Acenaphthene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005m /I ❑ ML
g ❑ MDL
Acenaphthylene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ ML
❑ MDL
Anthracene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑ MDL
Benzidine <0.085 mg/I <0.085 mg/I 3 EPA 625 .085mg/I ❑ ML
0 MDL
Benzo(a)anthracene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ ML
❑ MDL
Benzo(a)pyrene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑MDL
3,4-benzofluoranthene <0.01 mg/I <0.01 mg/I 3 EPA 625 .01mg/I ❑ML
❑MDL
EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
NC0020451 Town of West 001 OMB No.2040-0004
Jefferson
TABLE C. EFFLUENT PARAMETERS FOR SELECTED
POTWS
Maximum Daily Discharg) Average Daily Discharge Analytical ML or MDL
Pollutant Number of Method' (include units)
Value Units Value Units Samples
Benzo(ghi)perylene <0.015 mg/I <0.015 mg/I 3 EPA 625 .015mg/I ❑ ML
❑ MDL
Benzo(k)fluoranthene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ ML
❑ MDL
Bis (2-chloroethoxy) <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
methane 0 MDL
EPA Form 3510-2A(Revised 3-19) Page 23
Bis (2-chloroethyl)ether <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑ MDL
Bis (2-chloroisopropyl) <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
ether ❑ MDL
Bis (2-ethylhexyl) <0.08 mg/I <0.08 mg/I 3 EPA 625 .08mg/I 0 ML
phthalate 0 MDL
4-bromophenyl phenyl <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
ether 0 MDL
Butyl benzyl phthalate <0.01 mg/I <0.01 mg/I 3 EPA 625 .01mg/I 0 ML
❑ MDL
2-chloronaphthalene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑ MDL
4-chlorophenyl phenyl <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ ML
ether ❑ MDL
Chrysene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ML
❑ MDL
di-n-butyl phthalate <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑ MDL
di-n-octyl phthalate <0.03 mg/I <0.03 mg/I 3 EPA 625 .03mg/I 0 ML
❑MDL
Dibenzo(a,h)anthracene <0.05 mg/I <0.05 mg/I 3 EPA 625 .05mg/I ❑ ML
❑ MDL
1,2-dichlorobenzene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ML
0 MDL
1,3-dichlorobenzene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
0 MDL
1,4-dichlorobenzene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑MDL
3,3-dichlorobenzidine <0.06 mg/I <0.06 mg/I 3 EPA 625 .06mg/I ❑ML
0 MDL
Diethyl phthalate <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑MDL
Dimethyl phthalate <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ML
0 MDL
2,4-dinitrotoluene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ML
0 MDL
2,6-dinitrotoluene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ML
g 0 MDL
EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved
NC0020451 Town of West 001 03/05/19
Jefferson OMB No.2040-0004
EPA Form 3510-2A(Revised 3-19) Page 24
TABLE C. EFFLUENT PARAMETERS FOR SELECTED
POTWS
Maximum Daily Discharg: Average Daily Discharge
Pollutant Analytical ML or MDL
Value Units Value Units Number of Method' (include units)
Samples
1,2-diphenylhydrazine <0.02 mg/I <0.02 mg/I 3 EPA 625 .02mg/I ❑ ML
❑MDL
Fluoranthene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑MDL
Fluorene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ML
0 MDL
Hexachlorobenzene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑ MDL
Hexachlorobutadiene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ ML
❑MDL
Hexachlorocyclo- <0.06 mg/I <0.06 mg/I 3 EPA 625 .06mg/I 0 ML
pentadiene 0 MDL
Hexachloroethane <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ML
❑MDL
Indeno(1,2,3-cd)pyrene <0.014 mg/I <0.014 mg/I 3 EPA 625 .014mg/I ❑ML
❑ MDL
Isophorone <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑ MDL
Naphthalene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ML
0 MDL
Nitrobenzene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ ML
0 MDL
N-nitrosodi-n- <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
propylamine 0 MDL
N-nitrosodimethylamine <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑MDL
N-nitrosodiphenylamine <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I ❑ML
0 MDL
Phenanthrene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑ MDL
1 Sampling shall be conducted according to sufficiently sensitive test procedures (i.e., methods)approved under 40 CFR 136 for the analysis of
pollutants or pollutant parameters or required under 40 CFR Chapter I, Subchapter N or O. See instructions and 40 CFR 122.21(e)(3).
EPA Form 3510-2A(Revised 3-19) Page 25
PyreneI
<0.005 mg/I <0.005 mg/I 3 EPA 625 005mg/I 171
ML
❑MDL
1,2,4-trichlorobenzene <0.005 mg/I <0.005 mg/I 3 EPA 625 .005mg/I 0 ML
❑MDL
EPA Form 3510-2A(Revised 3-19) Page 26
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• 40, ,,,e _ 2&4 _24,4:4,4,2*.1 4:"'..-,-,4%.--",•M4.— . ..e.' !I.A1'. - '''' " A - -
i
• PO Box 7565
16 ` - Asheville,NC 28802
II
S Phone: (828)350-9364
;v..,.. ? Fax: (828)350-9368
''-', 1 Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: April 22,2016
Facility: Water Quality - NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Solutions,Inc. Comments:
Signature of Operator in Responsible Charge: eva
Signature of Laboratory Supervisor: 04/K.M.tO. Project: 11351
Samples: 160405.24,160407.26
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 04-06-16 04-13-16 1447 0755
Sample Information Sample 1 Sample 2 Control
Collection start date: 04-04-16 04-06-16 7. Test Information Start Renewal 1 Renewal 2 Stan Renewal 1 Renewal 2
Grab: Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24-h 24-h Initial pH(SU): 7.38 7.46 7.45 7.32 7.17 7.28
Alkalinity(mg/L CaCO3): 30,34 Final pH(SU): 7.49 7.51 7.45 7.17 7.23 7.33
Hardness(mg/L CaCO3): 38,36 Initial DO(mg/L): 8.0 8.0 7.9 7.7 7.8 7.9
Conductivity(µmhos/cm): 497 486 159,161.157 Final DO(mg/L): 8.1 8.0 8.0 7.9 7.9 7.8
Total residual chlorine(mg/L): <0.10 <0.10 Initial Temp.(°C): 24.8 25.0 25.1 24.7 24.8 24.8
Sample Temp.at Receipt CC): 2.6 4.2 Final Temp.(°C): 25.1 24.9 24.8 25.1 25.0 24.9
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of Young Produced 26 27 26 29 26 28 29 27 31 28 30 28 27.9 1 Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)ead L L L L LLL L L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 5.8
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Control: 0 of 12
Number of Young Produced 31 38 35 33 34 33 30 31 32 32 31 30 32.5 IwC: 0 of 12
Adult Survival: (L)ive,(D)ead L L L L HE, L L L L L L L -16.4 Significant?: No
Redocuon Final Mortality Significant at: No conc.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D)ead ' Reproduction NOEC: 56.4%
Red°aion Overall Method: Homoscedastic t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Method: Shapiro-Wilk's
Number of Young Produced _ Statistic: 0.927
Adult Survival: (L)ive,(D)ead Critical Value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 2.040
Number of Young Produced _ Critical Value: 5.320
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
%Reduction
Method:
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean 56.4% .
Number of Young Produced
Adult Survival: (L)ive,(D)ead
%Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev. 11/95
• PO Box 7565
• E T S
Asheville,NC 28802
• • Phone: (828)350-9364
Fax: (828)350-9368
3 Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form- Phase II Chronic Ceriodaphnia duhia Date: August 05,2016
Facility: Water Quality NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testing olution Inc. Comments:
Signature of Operator in Responsible Charge: 0 12A Cet e
Signature of Laboratory Supervisor: c ^0/ - Project: 11626
�\\/// Samples: 160719.11,160721.10
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 07-20-16 07-27-16 1057 0816
Sample Information Sample 1 Sample 2 Control
Collection start date: 07-18-16 07-20-16 Test Information start Renewal I Renewal 2 Start Renewal 1 Renewal 2
Grab: . . Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24-h 24-h Initial pH(SU): 7.83 7.85 7.87 7.68 7.75 7.70
Alkalinity(mg/L CaCO3): 32,33 Final pH(SU): 7.63 7.90 7.84 7.62 7.66 7.50
Hardness(mg/L CaCO3): 40,40 Initial DO(mg/L): 8.0 8.0 8.0 7.9 7.9 8.2
Conductivity(µmhos/cm): 516 526 155,152,156 Final DO(mg/L): 8.0 7.9 7.9 7.9 7.9 7.8
Total residual chlorine(mg/L): <0.10 <0 10 Initial Temp.(°C): 25.0 25.1 24.9 24.7 24.7 24.6
Sample Temp.at Receipt(°C): 2.5 0.6 Final Temp.(°C): 24.8 24.8 24.7 24.9 24.8 24.7
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of Young Produced 28 28 28 33 30 29 29 26 29 30 28 28 28.8 Final Control Mortality(%): 0 0
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 5.9
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mena Control: 0 of 12
Number of Young Produced 31 32 28 31 29 35 29 33 29 35 29 35 31.3 l WC: o of 12
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L_ -8.7 Significant?: No
v.R°duc6a° Final Mortality Significant at: No conc.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mc.„ Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 56.4%
Reduction Overall Method: Homoscedastic t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mren Method: Shapiro-Wilk's
Number of Young Produced Statistic: 0.926
Adult Survival: (L)ive,(D)ead Critical Value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 2.421
Number of Young Produced Critical Value: 5.320
•
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
Reduction
Method:
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1. 2 3 4 5 6 7 8 9 10 11 12 Mean 56.4% .
Number of Young Produced
Adult Survival: (L)ive,(D)ead
Redua,oe
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev. 11/95
'o PO Box 7565
4110
Asheville,NC 28802
0 Phone: (828)350-9364
._. 4-?...,;.:„.:1; Fax: (828)350-9368
Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-.Phase It Chronic Ceriodaphnia dubia Date: October 21,2016
Facility: Water Quality NPDES#: NC.0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Solutio -,Inc. Comments:
Signature of Operator in Responsible Charge: 1
Signature of Laboratory Supervisor: ..- Project; 11798
Samples: 16 1 004.19,161006.23
Mall Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Stan time End time:
Raleigh,NC 27699-1621 10-05-16 10-12-16 1248 0642
Sample Information Sample t. Sample 2 Control
Collection start date: 10-03-16 10-05-16 Test Information sen Renal I tttneend?. Sam Rn¢aat I RW,r,.,,a>
Grab: Treatment: 56.4% 56,4% 56.4% Control Control Control
Composite duration: 2I h 21 11Initial pH(SU): 7.46 7.65 7.59 7.34 7.55 7.44
Alkalinity(mg/L.CaCO)): 32,30,31 gins'pli(SU): 7.64 7.70 7.66 7,46 7.53 7.26
Hardness(m81L CaCO3): 44,42,40 Initial DO(mgit.): 79 8A 8<0 7.8 7.9 7.9
Conductivity(pmhoostcm): �ti al- 1eal.tei.157 Final DO(tng/L): 7.7 7.9 8.1 7.7 7.8 7.7
Total residual chlorine(mg/1..): 0 10 ,0 I 0 Initial Temp.(°C): 25.1 24,9 24.8 24.7 24.6 24.9
Sample Temp.at Receipt CC): 3 r. Final Temp.(°C); 24.9 25.1 24.9 24.9 24,9 25.2
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 I I 12 &WA Chronic Test Results
Number of Young Produced 29 29 28 31 28 32 29 31 31 27 29 29 29.4 Final Control Mortality(`6): 0.0
Adult Survival: (L)ive,(D)ead I. L L L_ L L L_ L L L 1,_ L %Control with 3rd Broods: 100
Control Reproduction CV: 5.1.
Effluent Percentage 56.4i) 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 n...n Control: 0 of 12
I Number of Young Produced 30 27 33 34 34 28 29 32 32 29 32 32 31.0 MC: 0 of 12
Adult Survival: (L)ive,(D)ead 1. L L 1_, _ L L, L L L L L L -5.4 Significant?: 14o
%ace`It° Final Mortality Significant at: No cone.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 '1 1 12 tkan Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 56.4%
::t c,en Overall Method: H4maostedastes t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms I 2 3 4 5 6 7 8 9 10 II 12 ;,,,,,n Method: Shapiro-wilts
Number of Young Produced Statistic. 0.963
Adult Survival: (L)ive,(D)ead Critical value: 0.884
Uqual Variances: Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 i 0 I I 12 s,�„.. Statistic: 2.408
Number of Young Produced Critical value: 5.320
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
Method:
Effluent Percentage' I Effluent 9'a Rank Stun Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Nem 56.4% ,
Number of Young Produced
Adult Survival: (:L)ive,(D)ead
'.Rab..wt
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% C'hV: >56.4%
DWQ form AT-3(8/91)Rev. 11/95
• PO Box 7565
• Asheville,NC 28802
1 . I 1 )T ''1 Phone: (828)350-9364
' w� f4�� Fax: (828)350-9368
Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: January 23,2017
Facility: Water Quality NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin, Solu'ons,Inc. Comments:
1 Signature of Operator in Responsible Charge: ( , //,,t
Signature of Laboratory Supervisor: im
•i Project: 12042
Samples: 170110.22,170112.30
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 01-11-17 01-18-17 1418 0652
Sample Information Sample 1 Sample 2 Control
Collection start date: 01-09-17 01-11-17 Test Information Stan Renewal 1 Renewal2 start Renewal 1 Renewal2
Grab: Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24-h 24-h Initial pH(SU): 7.69 7.49 7.55 7.22 7.33 7.39
Alkalinity(mg/L CaCO3): 31,32 Final pH(SU): 7.63 7.65 7.55 7.38 7.31 7.00
Hardness(mg/L CaCO3): 40,40 Initial DO(mg/L): 7.9 7.9 8.0 7.9 7.8 7.9
Conductivity(µmhos/cm): 570 580 lea.154,153 Final DO(mg/L): 8.0 8.0 7.9 7.8 7.8 7.8
Total residual chlorine(mg/L): <0.10 <0.10 Initial Temp.(°C): 24.8 24.7 24.7 24.6 24.6 24.7
Sample Temp.at Receipt(°C): 3.9 1 8 Final Temp.(°C): 25.2 25.0 24.9 25.0 24.9 25.1
4
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of Young Produced 27 25 26 27 30 31 26 28 27 27 29 28 27.6 Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 6.3
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms I 2 3 4 5 6 7 8 9 10 11 12 Mean Control: 0 of 12
Number of Young Produced 33 38 35 38 32 36 32 33 32 37 37 38 35.1 iwC: 0 of 12
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L -27.2 Significant?: No
Reduction Final Mortality Significant at: No conc.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Reproduction Analyses
Number of Young Produced - Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D)ead Reproduction NOEC 56.4%
v.Reduction Overall Method: Homoscedastic t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Method: Shapiro-Wilk's
Number of Young Produced Statistic: 0.934
Adult Survival: (L)ive,(D)ead Critical Value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 2.154
Number of Young Produced Critical Value: 5.320
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
%Reduction
Method: .
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean 56.4% .
Number of Young Produced
Adult Survival: (L)ive,(D)ead
%Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev.11/95
•
PO Box 7565
• Asheville,NC 28802
• Phone: (828)350-9364
to Fax: (828)350-9368
''''''',,.-..'-'4'''.' E-mail: Jim@etsnclab.com
y' Environmental Testing Solutions,Inc.
Date: February 22,2017
Effluent Aquatic Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test
Facility: Water Quality Laboratory NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testing Solu Aons,Inc. Comments:
Signature of Operator in Responsible Charge: (_�j,�q (,;a x
Signature of Laboratory Supervisor: �_ Project: 12099 '
Samples: 170206.03,170208.46,170210.29
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time: '
Raleigh,NC 27699-1621 02-07-17 02-14-17 0840 0757
Replicate number
1 2 3 4 Test Organisms
Control Surviving number of larvae 10 10 10 10 Survival(%) 100.0 Outside supplier:
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.611 I In-house Culture
Weight/original(mg/larvae) 0.499 0.694 0.649 0.600 Average wt/ 0.611
surviving(mg) Begin hatch: 02-06-17 1530
Effluent Surviving number of larvae 10 10 10 10 End hatch: 02-07-17 0615
14% Original number of larvae _10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.590 0.670 0.709 0.671 Average wt(mg) 0.660
%Effluent Surviving number of larvae 10 10 10 10
28% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.530 0.606 0.513 0.600 Average wt(mg) 0.562
%Effluent Surviving number of larvae 10 10 10 10
56.4% Original number of larvae - 10 - 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.620 0.536 0.569 0.603 Average wt(mg) 0.582
%Effluent Surviving number of larvae 10 10 10 10
78% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.558 0.592 0.500 0.542 Average wt(mg) 0.548
%Effluent Surviving number of larvae 6 5 6 6
100% Original number of larvae 10 10 10 10 Survival(%) 57.5
Weight/original(mg/larvae) 0.452 0.334 0.419 0.317 Average wt(mg) 0.381
Water Quality Data
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6
(
Initial 1 Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final
Control
pH(SU): 7.40 7.08 7.14 7.24 7.28 7.07 7.51 7.13 7.49 7.14 7.53 7.29 7.50 7.26
DO(mg/L): 7.9 7.5 7.7 7.4 7.8 7.1 8.0 7.1 7.8 6.9 7.8 7.3 7.9 7.6
Temp.(C): 24.8 24.6 24.7 24.4 24.6 24.5 24.7 24.6 24.6 24.5 24.8 24.7 24.8 25.1
High Concentration
pH(SU): 7.83 7.60 7.75 7.71 7.88 7.46 7.96 7.29 7.89 7.65 7.94 7.77 791 7.76
DO(mg/L): 8.1 7.5 7.8 7.5 8.0 7.6 8.1 7.7� 8.0 7.5 8.0 7.5 80 7.4
Temp.(°C): 25.0 24.3 24.8 24.3 25.0 24.4 24.6 24.5 24.8 24.4 25.0 24.5 25.0 24.7
Sample Information Sample 1 Sample 2 Sample 3 Control Analyses Survival Growth
Collection start date: 02-05-17 02-07-17 02-09-17 Normal: No Yes
Grab: Horn.Var. No Yes
Composite duration: 24-h 24-h 24-h NOEC: 78% 78%
Alkalinity(mg/L CaCO3): 83 80 80 30-32 LOEC: 100% 100%
Hardness(mg/L CaCO3): 100 100 98 40 ChV: 88.3% 88.3%
Conductivity(µmhos/cm): 564 571 594 144-158 Method: Steel's Dunnett's
Total residual chlorine(mg/L): <0.10 <0.10 <0.10
Sample Temp.at Receipt(°C): 1.2 0.7 1.2 Survival Growth
%Effluent Critical Calculated Critical Calculated
14% 10.000 18.000 2.360 -1.294
28% 10.000 18.000 2.360 1.262
56.4% 10.000 18.000 2.360 0.745
78% 10.000 18.000 2.360 1.634
Overall Analysis: l00% to.000 _ 10.000
Result: PASS LOEC: 100% NOEC: 78% ChV: 88.3%
DWQ form AT-5(8/03)
r
PO Box 7565
Asheville,NC 28802
* Phone: (828)350-9364
v1 '•ia : Fax: (828)350-9368
t., -=_ E-mail: Jimtetsnclab.com
,
Environmental Testing Solutions,Inc. Date: March 27,2017
Effluent Aquatic Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test
Facility: Water Quality Laboratory NPDES#: NC-0020451 Pipe#: 001 County: Mite -
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin.Solutions,In•. Comments:
Signature of Operator in Responsible Char e: 7777,4_ , %/
Signature of Laboratory Supervisor: Ml.. . Projeta: 12176
Samples: 170306.02,170308.36.170310.30
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQI Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time
Raleigh,NC 27699-1621 03-07-17 03-14-17 0851 0812
Replicate number
1 2 3 4 Test Organisms
Control Surviving number of larvae 10 10 10 10 Survival(%) 100.0 Su tier:
Organ isms Original number aflame 10 10 10 10 Avenge wt(mg) 0.658 J In-house Culture I
Weight/original(mg/larvae) 0.639 0.697 0.637 0.658 Averagewt; 0.658
surviving(mg) Begin hatch: 03-06-17 1605
%Effluent Surviving number of larvae 10 10 10 10 End hatch: 03-07-17 0812
14% Original member of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.691 0.699 0.650 0.687 Average wt(mg) 0.682
%Effluent Surviving number of larvae 10 10 10 10
28% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mgtlarvae) 0.650 0.725 0.664 0.557 Average wt(mg) 0.649
%Effluent Surviving number of larvae 10 10 10 10
56.4% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.585 0.634 0.648 _ 0.572 Average wt(mg) 0.610
%Effluent Surviving number oflarvae - 10 10 10 10
78% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.685 0.706 0.778 0.705 ' Average wt(mg). 0.719
%Effluent Surviving number of larvae 9 8 8 8
100% Original number of larvae 10 10 10 10 Survival(%) 82.5
Weight/original(mg/larvae) 0.550 0.546 0.554_ 0.607 Average wt(mg) 0564
Water Quality Data
Day 0 Day I Day 2 Dry 3 4 Day 5 _ Day` 6
Initial 1 Final Initial I Final Initial I Final Initial I Final Initial Emil Initial I_ Final Final Initial Final
Control
pH(SU): 7.02 7.15 7.20 7.36 7.21 7.05 7.38 7.30 7.55 7.28 7.47 7.18 7.49 7.23
DO(mg/L). 7.9 7.9 7.8 7.8 7.9 7.6 8.0 7.4 7.8 7.4 8.0 7.6 8.0 7.7
Temp.CC): • 24.7 24.3 24.7 24.2 24.6 24.5 24.8 24.3 24.7 24.2 _ 24.6 24.4 24.6 24,2 •
High Concentration
pH(SU): 7.71 7.58 7.74 7.87 7.73 7.53 7.87 7.71 7.69 7.69 7.93 7.69 7.85 7.76
DO(mg14: 8.0 8.0 8.0 8.0 8.0 7.7 8.0 7.9 7.9 7.8 V 7.9 7.7 7.8 7.6
Temp.("C): 24.7 24"4 24.6 24.3 24.7 24.5 25.0 24.2 24.8 24.4 24.8 _ 24.6 24.8 24.6
Sample Information Sample I Sample2 Sample3 Control Analyses Survival Growth
Collection start date. 03-05-17 03-07-17 03-09-17 Nonml: No Yes
Grab: Hour.Var. No Yes
Composite duration: 24-6 24-h 24-h NOEC: 78% 78%
alkalinity(mg/1.CaCO.r1: 45 43 43 32-35 LOEC', 100% 100%
Hardness(mg1L CaCO1): 100 100 100 38.40 ChV: 88.3% 88.3%
Conductivity()unhoe/cm): 538 506 505 152-161 Method: Steel'>: Drmnett's
Total residual chlorine(mg,L): <0.10 <0.10 <0.10
Sample Temp.at Receipt("Cl: 0.6 0.6 5.9 Survival Growth
%Effluent Critical Calculated Critical Calculatd
14% 10.000 18.000 2.360 -0.796
28% 10.000 18.000 1360 0.290
. 56.4% 10.000 18.000 2.360 1.591
78% 10.000 1111.000 2,360 -2.014
Overall Analysis: t00% 10,000 lo.000 .
Result: PASS LOEC: 100% NOEC: 78% ChV: 88.3%
DWQ fame 4T-5(8/03)
• PO Box 7565
0 Asheville,NC 28802
1116
Phone: (828)350-9364
:''" - : 4 Fax: (828)350-9368
..,,) Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form - Phase II Chronic Ceriodaphnia dubia Date:
Facility: Water Quality NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Solutions,Inc. Continents:
Signature of Operator in Responsible Charge: .
Signature of Laboratory Supervisor: Project: 12266
. Samples: 170404 20,170406.25
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Stan date: End date: Stan time: End time:
Raleigh,NC 27699-1621 04-05-17 04-12-17 1446 0643
Sample Information Sample 1 Sample 2 Control
Collection start date: 04-03-I6 04-05-16 "Test Information - Su.. Rercwali Ren0ra12 sun Renewal l Rcnowal2
Grab: Treatment: 56.4% 56,4% 56,4% Control Control Control
Composite duration: 1-11 .1 Hi Initial pH(SU):. 6.95 7.64 7.37 7.11 7.47 7.17
Alkalinity(mg1L CaCO3): t I.?; Final pH(SU): 7.41 7.45 7.38 7.48 7.16 7.07
Hardness(nigil..CaCO3):' . .In it, Initial DO(mg/Li 7.8 7.9 7.9 7.8 8.0 7.8
Conductivity(pniliosicm): 136 458 i Final DCI(mg/L): 7.9 8.0 7,9 7.9 8.0 7.8
Total residual chlorine(mg/I.): <0.10 <0.10 Initial Temp.(°C): 24.9 24.7 24.7 24.7 24.6 24.7
Sample Temp.at Receipt(°C): 2.8 1.4 Final Temp.("C): 25.1 25.2 25.1 25.0 24.9 24.9
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 si Chronic Test Results
Number of Young Produced 27 26 25 26 28 26 29 26 27 27 28 29 27.0 Final Control Mortality(%): 0,0
Adult Survival: (L)ive,(D)ead L L L L L L_ L L L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 4.7
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 act,.. Control: 0 or 12
Number of Young Produced 34 33 34 30 32 32 29 28 32 33 33 29 31.6 iwc: 0 of 12
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L -17.0 Significant?: Ni3
Reduction Final Mortality Significant al No cone.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mon Reproduction Analyses
Number of Young Produced Reproduction LOU' >56.4%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 56.4%
x Reduction Overall Method: Homoscedesdc I
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 'Wen Method: Siutpern-wok's
Number of Young Produced Statistic: 0.955
Adult Survival: (L)ive.(D)ead Critical Value 0.884
%Rcdunim,
Equal Variances: yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 M.. Statistic: 2.606
Number of Young Produced _ _ Critical Value: 5.320
Adult Survival: (L)ive,(D)ead Non-ParainetricAnalysis(if applicable)
%Radiate.
Method:
Effluent Percentage Effluent% Rank Sun Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 me. 56.4%
Number of Young Produced
Adult Survival: (L)ive,(D)cad
%RedMion
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT--3(8/91)Rev.11/95
f• PO Box 7565
0 Asheville,NC 28802
. ETs Phone: (828)350-9364
Fax: (828)350-9368
E-mail: Jim@etsnclab.com
'Of► Environmental Testing Solutions,Inc. Date: April 28,2017
Effluent Aquatic Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test
1 Facility: Water Quality Laboratory NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin olutio s,Inc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: Project: 12246
Samples: 170403.02,170405.28,170407.15
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: _ End time:
Raleigh,NC 27699-1621 04-04-17 04-11-17 0905 0923
Replicate number
1 2 3 4 Test Organisms
Control Surviving number of larvae 10 10 10 10 Survival(%) 100.0 Source:
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.740 In-house Culture
Weight/original(mg/larvae) 0.754 0.650 0.706 0.851 Average wt/ 0.740
surviving(mg) Begin hatch: 04-03-17 1630
%Effluent Surviving number of larvae 10 10 10 10 End hatch: 04-04-17 0610
Ea Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.785 0.736 0.792 0.825 Average wt(mg) 0.785
%Effluent 'Surviving number of larvae 10 10 10 10
Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.821 0.724 0.767 0.773 Average wt(mg) 0.771
%Effluent Surviving number of larvae 10 10 10 10
EMI Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(rng/larvae) 0.830 0.839 0.772 0.730 Average wt(mg) 0.793
%Effluent Surviving number of larvae 10 10 10 10
EIS Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.809 0.763 0.845 0.855 Average wt(mg) 0.818
%Effluent Surviving number of larvae 10 10 10 9
100% Original number of larvae 10 10 10 10 Survival(%) 97.5
Weight/original(mg/larvae) 0.789 0.870 0.809 0.714 Average wt(mg) 0.796
Water Quality Data
Day 0 Day 1 Day 2 Da 3 Day 4 Day 5 Day 6
Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final
Control
pH(SU): 7.46 7.17 7.11 7.15 7.19 7.11 7.47 7.23 7.47 7.16 7.53 7.11 7.17 7.19
DO(mg/L): 7.9 7.8 7.8 7.7 7.8 7.2 8.0 7.4 7.9 7.6 8.0 7.7 7.8 6.5
Temp.(°C): 24.9 24.3 24.7 24.2 24.5 24.5 24.7 24.6 24.6 24.2 24.6 24.5 24.6 24.6
High Concentration
pH(SU): 7.74 , 7.53 7.63 7.48 7.57 7.43 7.73 _ 7.35 7.74 7.40 7.79 7.32 7.54 7.32
DO(rng/L): 8.0 7.9 7.9 7.7 7.9 7.5 7.9 7.4 7.8 7.6 8.0 8.0 8.0 6.6
Temp.(°C): 24.8 24.5 24.9 24.4 24.7 24.6 25.0 24.4 24.8 24.4 24.7 24.4 24.8 24.7
Sample Information Sample 1 Sample 2 Sample 3 Control Analyses Survival Growth
Collection start date: 04-02-17 04-04-17 04-06-17 Normal: Yes Yes
Grab: . . Horn.Var. Yes Yes
Composite duration: 24-h 24-h 24-h NOEC: 100% 100%
Alkalinity(mg/L CaCO3): 72 • 60 66 31-35 LOEC: >100% >100%
Hardness(mg/L CaCO1): 89 87 95 40-40 ChV: >100% >100%
Conductivity(µmhos/cm): 437 437 468 149-163 Method: Visual lnsp Dunnett's
Total residual chlorine(mg/L): <0.10 <0.10 <0.10
Sample Temp.at Receipt(°C): 1.0 0.3 3.1 Survival Growth
%Effluent Critical Calculated Critical Calculated
14% . 2.410 -1.121
28% . 2.410 -0.786
56.4% 2.410 -1.330
78% . 2.410 -1.970
Overall Analysis: 100% . 2.410 -1.400
Result: PASS LOEC: >100% NOEC: 100% ChV: >100%
DWQ fonn AT-5(8/03)
•41,
PO Box 7565
• Asheville,NC 28802
f• ETS
Phone: (828)350-9364
Fax: (828)350-9368
E-mail: Jim@etsnclab.com
3 Environmental Testing Solutions,Inc. Date: May 26,2017
Effluent Aquatic Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test
Facility: Water Quality Laboratory NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Solutions,Inc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: Project: 12331
Samples: 170501.02,170503.34,170505.16
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 05-02-17 05-09-17 0852 0926
Replicate number
1 2 3 4 Test Organisms
Control Surviving number of larvae 10 10 10 9 Survival(%) 97.5 Source:
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.621 In-house Culture
Weight/original(mg/larvae) 0.586 0.574 0.721 0.602 Average wt/ 0.637
surviving(mg) Begin hatch: 05-01-17 1700
%Effluent Surviving number of larvae 10 10 10 10 End hatch: 05-02-17 0605
ERE Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.709 0.632 0.767 0.786 Average wt(mg) 0.724
%Effluent Surviving number of larvae 10 10 10 10
BIM Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.605 0.605 0.662 0.646 Average wt(mg) 0.630
%Effluent Surviving number of larvae 10 10 10 9
56.4% Original number of larvae 10 10 10 10 Survival(%) 97.5
Weight/original(mg/larvae) 0.603 0.608 0.587 0.563 Average wt(mg) 0.590
%Effluent Surviving number of larvae 10 10 10 10
78% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.636 0.671 0.686 0.694 Average wt(mg) 0.672
%Effluent Surviving number of larvae 10 10 10 10
100% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.575 0.733 0.726 0.605 Average wt(mg) 0.660
Water Quality Data
Day 0 Day I Day 2 Day 3 Day 4 Day 5 Day 6
/ r
Initial I Final Initial 1 Final Initial I Final Initial I Final Initial I Final Initial I Final Initial 1 Final
Control
pH(SU): 7.94 7.78 7.81 7.49 7.77 7.40 7.77 7.59 7.85 7.34 7.84 7.60 7.84 7.37
DO(mg/L): 7.9 7.7 7.8 7.0 7.7 7.0 7.8 8.0 8.0 6.6 7.9 7.7 7.8 6.6
Temp.(°C): 24.7 24.6 24.7 24.6 24.7 24.3 24.7 24.2 24.7 24.6 24.8 24.5 24.6 24.5
High Concentration
pH(SU): 8.13 7.92 8.08 7.72 7.98 7.51 8.00 7.75 8.04 7.50 8.15 7.87 8.10 7./1
DO(mg/L): 8.0 7.7 7.7 6.8 7.8 7.2 7.9 7.5 8.4 6.4 8.1 7.8 8.0 7.6
Temp.(°C): 25.0 24.5 24.9 24.4 24.6 24.5 24.9 24.5 25.0 24.4 25.0 24.5 24.7 24.7
Sample Information Sample I Sample 2 Sample 3 Control Analyses Survival Growth
Collection start date: 04-30-17 05-02-17 05-04-17 Normal: Yes Yes
Grab: . . . Horn.Var. Yes Yes
Composite duration: 24-h 24-h 24-h NOEC: 100% 100%
Alkalinity(mg/L CaCO3): 69 67 63 35-35 LOEC: >100% >100%
Hardness(mg/L CaCO3): 90 80 92 40-40 ChV: >100% >100%
Conductivity(µmhos/cm): 451 381 448 156-170 Method: Visual Insp Dunnetfs
Total residual chlorine(mg/L): <0.10 <0.10 <0.10
Sample Temp.at Receipt(°C): 4.1 4.0 2.4 Survival Growth
%Effluent Critical Calculated Critical Calculated
14% . 2.410 -2.657
28% 2.410 -0.226
56.4% . . 2.410 0.789
78% . . 2.410 -1.319
Overall Analysis: l00% 2.410 -1.008
Result: PASS LOEC: >100% NOEC: 100% ChV: >100%
DWQ form AT-5(8/03)
f
• 203., A.
PO Box 7565
* ETs 'ty ��' Asheville,NC 28802
Phone: 828 350-9364
Fax: (828)350-9368
Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: July 24,2017
Facility: Water Quality NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testis olutyQ s,Inc. Comments:
Signature of Operator in Responsible Charge: ff//{ C�f9
Signature of Laboratory Supervisor: Project: 12513
Samples: 170711.20,170713.20
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 07-12-17 07-19-17 1342 0741
Sample Information Sample 1 Sample 2 Control
Collection start date: 07-11-17 07-13-17 Test Information start Renewal 1 Renewal Stan Renewal 1 Renewal 2
Grab: X X Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: Initial pH(SU): 7.74 7.70 7.86 7.67 7.69 7.74
Alkalinity(mg/L CaCO3): 33,33 Final pH(SU): 7.81 7.93 7.79 7.77 7.81 7.52
Hardness(mg/L CaCO3): 40,40 Initial DO(mg/L): 8.0 7.9 8.0 7.9 7.9 7.9
Conductivity(pmhos/cm): 468 472 159.156,156 Final DO(mg/L): 8.0 8.1 7.8 7.7 8.0 7.6
Total residual chlorine(mg/L): <0.10 <0.10 Initial Temp.CC): 24.7 24.8 24.7 24.9 24.8 24.6
Sample Temp.at Receipt(°C): 3.4 0.9 Final Temp.CC): 25.2 24.9 24.7 24.9 25.0 24.8
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of Young Produced 29 26 27 28 29 27 28 33 28 32 27 28 28.5 Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 7.3
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Control: o of 12
Number of Young Produced 29 34 34 37 29 35 32 35 30 34 31 30 32.5 IWC: 0 of 12
Adult Survival: (L)ive,(D)ead L L L L L L L L L L , L L -14.0 Significant?: No
Reduction Final Mortality Significant at: No conc.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 56.4%
Reduction Overall Method: Homoscedastic t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Method: Shapiro-Wilk's
Number of Young Produced Statistic: 0.945
Adult Survival: (L)ive,(D)ead Critical Value: 0.884
%Reduction
Equal Variances: " Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 1.681
Number of Young Produced Critical Value: 5.320
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
%Reduction
Method:
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean 56.4% .
Number of Young Produced
Adult Survival: (L)ive,(D)ead
%Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev. 11/95
7
• PO Box 7565
Asheville,NC 28802
� J Phone: (828)350-9364
ETS Fax: (828)350-9368
•
3 Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: October 20,2017
Facility: Water Quality NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Soluti ns,Inc. Comments:
Signature of Operator in Responsible Charge: L/l-.r►,{
Signature of Laboratory Supervisor: Project 12744
Samples: 171003.23,171005.24
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 10-04-17 10-11-17 1545 0710
Sample Information Sample 1 Sample 2 Control
Collection start date: 10-02-17 10-04-17 Test Information Stan Renewal 1 Renewal 2 Stan Renewal 1 Renewal 2
Grab: Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24-h 24-h Initial pH(SU): 7.48 7.60 7.48 7.23 7.28 7.10
Alkalinity(mg/L CaCO3): 35,35 Final pH(SU): 7.63 7.53 7.51 7.29 7.22 7.05
Hardness(mg/L CaCO3): 40,38 Initial DO(mg/L): 7.9 7.9 7.9 7.8 8.0 8.0
Conductivity(pmhos/cm): 506 508 152,159,161 Final DO(mg/L): 7.7 8.0 8.0 7.7 7.7 7.7
Total residual chlorine(mg/L): <0.10 <0.10 Initial Temp.(°C): 25.0 24.8 25.2 24.8 24.8 24.9
Sample Temp.at Receipt(°C): 0.9 0.5 Final Temp.(°C): 25.1 24.9 24.9 25.0 24.9 25.0
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of Young Produced 29 27 31 31 33 30 29 31 31 29 34 29 Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L 30.3 %Control with 3rd Broods: 100
Control Reproduction CV: 6.3
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mein Control: 0 of 12
Number of Young Produced 27 29 27 28 28 29 32 28 29 26, 28 28 28.3 IWC: 0 of 12
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L 6.9 Significant?: No
i Reduction Final Mortality Significant at: No conc.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4% '
Adult Survival: (L)ive,(D)ead Reproduction NOEL: 56.4%
Reduction Overall Method:
Homoscedastic t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Meta Method: Shapiro-Wilk's
Number of Young Produced Statistic: 0.921
Adult Survival: (L)ive,(D)ead Critical value: 0.884
Reduction
Equal Variances: Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 1.677
Number of Young Produced Critical Value: 5.320
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
Y Reduction
Method:
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Meat. 56.4%
Number of Young Produced
Adult Survival: (L)ive,(D)ead
Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev. 11/95
PO Box 7565
... ETs Asheville,NC 28802
,it
• Phone: (828)350-9364
i-1,4 Fax: (828)350-9368
Environmental Testing Solutions,Inc.
11 Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: April 20,2018
Quality
Facility:Y Q ty NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testing Soluti ns,Inc. Comments:
Signature of Operator in Responsible Charge: Cywora (3i�
Signature of Laboratory Supervisor: d
,� Project: 13208
Samples: 180403.20,180405.28
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 04-04-18 04-11-18 1338 0643
Sample Information Sample 1 Sample 2 Control
Collection start date: 04-02-18 04-04-08 Test Information Start Renewal 1 Renewal 2 Sun Renewal I Renewal 2
Grab: Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24-h 24-h Initial pH(SU): 7.66 7.89 8.01 7.77 7.76 7.79
Alkalinity(mg/L CaCO3): 32,33 Final pH(SU): 7.88 8.04 8.02 I 7.72 7.77 7.66
Hardness(mg/L CaCO3): 42,40 Initial DO(mg/L): 8.2 8.0 8.0 7.7 7.7 7.8
Conductivity(pmhos/cm): 320 440 150 148.153 Final DO m( g/L): 8.1 8.1 8.0 7.9 7.6 7.5
Total residual chlorine(mg/L): <0.10 <0.10 Initial (°
Temp. C
p ) 25.0 24.8 24.7 24.8 24.8 24.7
Sample Temp.at Receipt(°C): 1.8 2.0 Final Temp.(°C): 25.2 25.0 24.8 25.1 25.0 24.8
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of Young Produced 29 32 29 30 32 31 29 30 28 28 31 27 Final Control Mortality(%): 0.0
29.7
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 5.4
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mae, Control: 0 of 12
Number of Young Produced 34 33 32 31 32 36 30 33 33 29 30 31 32.0 IwC: o of 12
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L -7.9 Significant?: No
RedoC°O° Final Mortality Significant at: No conc.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Meant Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 56.4%
%ReduCUon Overall Method: Homoscedastic t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Maas Method: Shapiro-Wilk's
Number of Young Produced Statistic: 0.979
Adult Survival: (L)ive,(D)ead Critical value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 1.465
Number of Young Produced Critical value: 5320
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
V.Reduction
Method:
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean 56.4% .
Number of Young Produced
Adult Survival: (L)ive,(D)ead
Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev. 11/95
, ,, • PO Box 7565
, .... , ..... ig Asheville,NC 28802
. ' : ETS
Phone: (828)350-9364
Fax: 828( )350-9368
3 Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: January 26,2018
Facility: Water Quality NPDES#: NC.0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Solutions,Inc. Comments:
Signature of Operator in Responsible Charge: ii2e l `
Signature of Laboratory Supervisor: vr /�,g Project: 12967
Samples: 180109.20,180111.29
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 01-10-18 01-17-18 1418 0743
Sample Information Sample 1 Sample 2 Control
Collection start date: 01-08-18 01-10-18 Test Information scan Rem-,..,t Renewal 2 Sian Rout t Renewal 2
Grab. Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24-h 24-h Initial pH(SU): 7.95 7.88 8.06 7.61 7.69 7.87
Alkalinity(mg/L CaCO3): 35,34 Final pH(SU): 7.90 8.09 8.06 7.66 7.83 7.77
Hardness(mg/L CaCO3): 42,40 Initial DO(mg/L): 8.0 8.3 8.0 7.8 7.7 7.8
Conductivity(pmhos/cm): 536 520 152,151,150 Final DO(mg/L): 8.2 8.2 8.1 7.9 8.1 7.7
Total residual chlorine(mg/L): <0.10 <0.10 Initial Temp.(°C): 24.6 25.0 24.8 24.7 24.8 24.9
Sample Temp.at Receipt(°C): 13 0.6 Final Temp.(°C): 25.0 25.1 24.9 25.1 24.9 24.7
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of Young Produced 30 28 28 29 29 28 30 33 31 30 28 28 29.3 Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)ead L L L L L L L L _ L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 5.3
Effluent Percentage 56.4% 48 Hour Mortality
Treatm
ent 2 Organisms 1 2 3 4, 5 6 7 8 9 10 11 12 Mean Control: 0 of 12
Number of Young Produced 36 39 33 32 34 36 32 36 35 34 32 37 34.7 IWC: o of 12
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L -18.2 Significant?: No
C1O° Final Mortality Significant at: No conc.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 56.4%
Reduction Overall Method: Homoscedastic I
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Method: Shapiro-Wilk's
Number of Young Produced Statistic: 0.946
Adult Survival: (L)ive,(D)ead Critical Value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 2.050
Number of Young Produced _ Critical Value: 5.320
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
%Reduction
Method:
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean 56.4%
Number of Young Produced
Adult Survival: (L)ive,(D)ead
%Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev.11/95
. p. ETs
* PO Box 7565
• Asheville,NC 28802
Phone: (828)350-9364
Fax: (828)350-9368
~ Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: July 27,2018
Facility: Water Quality NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testi lutions Inc. mments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: Project: 13427
Samples: 180710.27,180712.31
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 07-11-18 07-18-18 1318 0726
Sample Information Sample I Sample 2 Control
Collection start date: 07-09-18 07-11-18 Test Information Sun Renewal t Renewal 2 Stan Renew.'1 Renewal
Grab: ! Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24-h 24-h Initial pH(SU): 7.27 7.30 7.43 7.27 7.25 7.44
Alkalinity(mg/L CaCO3): 30,30,30 Final pH(SU): 7.29 7.46 7.16 7.15 7.30 6.93
Hardness(mg/L CaCO3): 40,40,38 Initial DO(mg/L): 7.9 7.9 8.0 7.9 7.8 7.8
Conductivity(µmhos/cm): 376 392 lso,155,150 Final DO(mg/L): 7.8 8.1 8.2 8.0 7.9 8.0
Total residual chlorine(mg/L): <0.10 <0.10 . Initial Temp.(°C): 24.8 24.9 25.0 24.8 24.7 24.8
Sample Temp.at Receipt(°C): 1.0 0.4 Final Temp.(°C): 25.2 24.8 24.9 24.9 25.1 25.0
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of YoungProduced 29 29 31 31 30 31 30 31 30 29 28 29 Final Control Mortality % 0.0
IIIIIIIIIIII129.8O
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L ( %Control with 3rd Broods: 100
Control Reproduction CV: 3.5
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 man Control: 0 of 12
Number of Young Produced 35 30 29 36 33 31 36 32 35 33 31 32.8 IWC: 0 of 12
Adult Survival: (L)ive,(D)ead I L I L I L I L I L I L I L I L 133 L I L I L I L I -10.1 I Significant?: No
%Reduction
Final Mortality Significant at: No conc.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Man Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 56.4%
ned0a O° Overall Method:
Homosr.edastic t
Effluent Percentage
Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mao Method:
(L)ive,
) ( ) I I I I I I I I I I I Shapiro-WillesNumber of Young Produced 0.
Statistic: 0.967
Adult Survival: Live, Dead
Critical Value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage Method.
F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Man Statistic:
5.114
Number of Young Produced Adult Survival: (L)ive,(D)eadI I I I I I I I I I I I I
Critical Value: 5.320
Non-Parametric Analysis(if applicable)
Reduction
Method:
Effluent Percentages s Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Man 56.4%
Number of Young Produced
Adult Survival: (L)ive,(D)ead l l l l l l l l l l l l l
°.Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev.11/95
•
PO Box 7565
r
ill
Asheville,NC 28802
0 E Mir lib 4 `/ Phone: (828)350-9364
Fax: (828)350-9368
Environmental Testing Sol Ions,Inc.
Effluent Aquatic Toxic ty Report Form-Phase II Chr i is Ceriodaphnia dubia Date: October 22,2018
Facility: Water Qual NP liES#: NC-0020451 Pipe#: 001 County: Ashe
West Je erson WWTP
Laboratory Performin: Test: Environmental Testin Solutions,I Comments:
Signature of Operator n Responsible Charge:
Signature of Iaborato l Supervisor: jot Project: 13617
Samples: 181002.11,181004.11
Mail Original To: 1 orth Carolina Department of Environment and Natural Resources
l WQ/Environmental Sciences Branch
621 Mail Service Center Start date: End date: Start time: End time:
aleigh,NC 27699-1621 10-03-18 10-10-18 1211 0634
Sample Informatio Sample 1 Sample 2 Control
Collection start date: 10-01-18 10-03-18 Test Information Sun Renewal I Rrnewd 2 Stan Renewal 1 Rrxwd 2
Grab: Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24-h 24-h Initial pH(SU): 7.65 7.60 7.61 7.34 7.25 7.22
Alkalinity(mg/L CaIO3): 30,30 Final pH(SU): 7.67 7.68 7.77 7.26 7.34 7.44
Hardness(mg/L CaC a 3): 40,40 Initial DO(mg/L): 8.1 8.1 8.4 7.6 7.6 7.7
Conductivity(girth. an): 461 482 Ise,iss,tso Final DO(mg/L): 8.3 8.2 8.5 7.6 7.8 7.7
Total residual chlo' (mg/L): <0.10 <0.10 Initial Temp.(°C): 25.0 24.9 24.8 24.6 24.6 24.7
Sample Temp.at R-..pt(°C): 3.2 2.9 Final Temp.(°C): 24.9 25.2 25.0 25.0 25.I 25.2
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of Young Produced 26 30 25 25 27 30 28 26 26 24 27 27 Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)e.. L L L L L L L L L L L L 26.8 %Control with 3rd Broods: 100
Control Reproduction CV: 7.0
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mm. Control: 0 of 12
Number of Young Produced 30 27 30 27 25 28 31 28 28 28 27 28 28.1 IwC: 0 of 12
Adult Survival: (L)ive,(D)e:. L L L L L L L L L L L L -5.0 Significant?: No
x Reduction Final Mortality Significant at: No conc.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Man Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D .•. Reproduction NOEC: 56.4%
%Reduction Overall Method:
Hotno.cedutic t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Method: Shapiro-Wilk's
Number of Young Produced Statistic: 0.942
Adult Survival: (L)ive,(D)e.. Critical value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 1.323
Number of Young Produced Critical value: 5.320
Adult Survival: (L)ive,(D)e.. Non-Parametric Analysis(if applicable)
%Reduction
Method:
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 me. 56.4%
Number of Young Produced _
Adult Survival: (L)ive,(D)e..
%Reduction
Overall Analysis:
Result: PASS LO C: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev. 1/95
PO Box 7565
I
Asheville,NC 28802
'
Phone: (828)350-9364
Fax: (828)350-9368
•42) Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: January 25,2019
Facility: Water Quality NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
LaboratoryPerformingTest: Environmental Testin Solutio Inc. Comments:
��'
Signature of Operator in Responsible Charge: r '.-
Signature of Laboratory Supervisor: to' ect: 13883
Samples: 190108.25,190110.25
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 01-09-19 01-16-19 1431 0659
Sample Information Sample 1 Sample 2 Control
Collection start date: 01-07-19 01-09-19 Test Information sun Ra .l I a ale sun Renewal 1 R�.12
Grab: 1 -- Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24-h 24-h Initial pH(SU): 8.03 8.01 8.07 7.80 7.79 7.81
Alkalinity(mg/L CaCO3): 33,32 Final pH(SU): 8.05 8.10 8.00 7.74 7.84 7.74
Hardness(mg/L CaCO3): 34,34 Initial DO(mg/L): 8.2 7.9 8.1 7.8 8.0 8.1
Conductivity(µmhos/cm): 436 470 156,152,150 Final DO(mg/L): 8.1 8.2 8.3 8.0 8.0 8.1
Total residual chlorine(mg/L): <0.10 <0.10 Initial Temp.(°C): 25.2 24.7 24.6 24.8 24.8 24.7
Sample Temp.at Receipt(°C): 1.4 0.9 Final Temp.(°C): 25.0 25.1 25.0 25.1 25.0 25.0
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Man Chronic Test Results
Number of Young Produced 28 35 30 30 32 31 29 30 34 31 30 33 31.1 Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L %Control with 3rd Broods: 100
Control Reproduction CV: 6.6
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Man Control: o of 12
Number of Young Produced 32 34 35 31 32 33 34 33 32 27 37 35 32.9 IwC: 0 of 12
Adult Survival: (L)ive,(D)ead L L L . L L , L L L L L L L -5.9 Significant?: No
%RedufA 0n Final Mortality Significant at: No cone.
Effluent Percentage..
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Man Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 56.4%
v RedoC4an Overall Method: Homoscedastic t
Effluent Percentage- Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Man Method: Shapiro-Wilk'.
Number of Young Produced _ Statistic: 0.958
Adult Survival: (L)ive,(D)ead Critical Value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage' Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Man Statistic: 1.469
Number of Young Produced Critical Value: 5.320
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
x Redua,a Method:
Effluent Percentage- Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean 56.4%
Number of Young Produced
Adult Survival: (L)ive,(D)ead
%Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev. 11/95
l
•
•
PO Box 7565
11111
Asheville,NC 28802
Illfr
CPhone: (828)350-9364
F
(828)
8
EmlronmentalTesting5olutions, @ets club c rn
-- E-mail: Jim@etsnclab.com
Inc.c.
Date: April 22,2019
Effluent Aquatic Toxicity Report Form-Chronic Fathead Minnow ulti-Concentration Test
Facility: Water Quality Lab&Operations,Inc. NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Solutions,Inc Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: _
/ Project-. 14082
Samples: 190401.01,190403.31,190405.24
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 04-02-19 04-09-19 0836 0743
Replicate number
1 2 3 4 Test Organisms
Control Surviving number of larvae 10 10 10 10 Survival(%) 100.0 Source:
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.787 I In-house Culture I
Weight/original(mg/larvac) 0.881 0.802 0.803 0.663 Average wt/ 0.787
surviving(mg) Begin hatch: 04-01-19 1310
Effluent Surviving number of larvae 10 10 10 1 10 End hatch: 04-02-19 0610
14% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.757 0.759 0.812 0.852 Average wt(mg) 0.795
%Effluent Surviving number of larvae 10 I
10 10 I 10
28% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.793 0.842 0.816 0.966 Average wt(mg) 0.854
Effluent Surviving number of larvae 10 10 10 10
156.4%I Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.755 0.845 0.819 0.790 Average wt(mg) 0.802
`,...._" %Effluent Surviving number of larvae 10 10 10 10
78% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.752 0.758 0.787 0.768 Average wt(mg) 0.766
%Effluent Surviving number of larvae 9 10 10 9
100% Original number of larvae 10 10 10 10 Survival(%) 95.0
Weight/original(mg/larvae) 0.703 0.793 0.795 0.683 Average wt(mg) 0.744
Water Quality Data
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6
Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final
Control
pH(SU): 7.85 7.64 7.79 7.44 7.56 7.43 7.58 7.42 7.75 7.31 7.73 7.55 7.74 7.50
DO(mg/L): 8.0 7.8 8.1 7.7 8.0 7.6 8.1 7.1 8,l 7.2 8.0 7.6 7.9 7.3
Temp.(`C): 24.8 24.5 24.7 24.5 24.7 24.3 24.7 24.5 24.7 24.5 24.7 24.7 24.8 24.7
High Concentration
pH(SU): 8.20 7.90 8.11 7.71 8.02 7.59 8.08 7.61 8.08 7.53 8.05 7.74 8.04 7.74
DO(mg/L): 8.l 7.8 8.3 7.6 8.3 7.5 8.3 7.0 8.2 6.8 8.2 7.1 8.2 7.3
Temp.(°C): 25.0 24.3 24.9 24.6 25.0 24.5 24.8 24.6 25.0 24.3 24.7 24.7 25.0 24.6
•
Sample Information Sample 1 Sample 2 Sample 3 Control Analyses Survival Growth
Collection start date: 03-31-19 04-02-19 04-04-19 Normal: Yes Yes
Grab:
Horn.Var. Ycs Yes
Composite duration: 24-h 24-h 24-h NOEC: 100% 100%
Alkalinity(mg/L CaCO3): 77 73 74 34,35 LOEC: >100% >100%
Hardness(mg/L CaCO3): 90 90 110 42,42 ChV: >100% >100%
Conductivity(pmhos/cm): 424 422 429 153-167 Method: Visual Insp. Dunnett's
i Total residual chlorine(mg/L): <0.10 <0.10 <0.10
Sample Temp.at Receipt('C): 0.6 2.1 1.9 Survival
Growth
%Effluent Critical Calculated Critical Calculated •
14% . . 2.410 -0.183
28% . 2.410 -1.584
56.4% 2.410 -0.355
�- 78% 2.410 0.496
Overall Analysis: 100
2.410 1.034
Result: PASS LOEC: >100% NOEC: 100% ChV: >100%
DWQ form AT-5(8/03)
•
• PO Box 7565
t� ,
Phone:Asheville(828)NC 350-936428802
' Fax: (828)350-9368
Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: April 22,2019
Facility: Water Quality NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Solutio s,I Comments:
Signature of Operator in Responsible Charge: j
Signature of Laboratory Supervisor: Project 14103
Samples: 190102.25,190404.24
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date, End date: Start time: End time: I
Raleigh,NC 27699-1621 I 04-03-19 04-10-19 1320 ' 0651
Sample Information Sample 1 Sample 2 Control
Collection start date. 04-01-19 04-03-19 �. Test Information Start Renewal I Renewal 2 Start Renewal I Renewal 2
Grab:
if-I Treatment 56.4% 56.4% 56.4% Control Control Control
Composite dinanon: 24 h 24 h 4!i r i Initial pH(SU): 7.91 7.75 7.92 7.79 7,58 7.74
Alkalinity(mg/L CaCO3): ` :
_iy s v .Est 34,35 Final pH(SU): 7.95 7.96 7.89 7.68 7,71 7.63
Hardness(mg/L CaCO3): ��T Y` " ' 42,42 Initial DO(m
g/L):+�-c ( g/L): 8.1 8.1 8.1 8.1 8 1 7.9
Conductivity(pmhos/cm): 419 445 154.157.167 Final DO(mg/L): 8.3 8.1 8.2 8.1 8.0 8.0
Total residual chlorine(mg/L): . '
�) <0 10 <0.10
Initial Temp.CC): 24.8 24.8 25.0 24.7 24.7 24.9
Sample Temp.at Receipt CC): 3.8 1.5litAja: Final Temp.CC): 25.1 25,2 24.9 24,9 25.1 25,0
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Me,. Chronic Test Results
!Number of Young Produced 29 25 24 29 29 30 27 29 26 26 28 28 Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)ead I L I L I L L I L I L I L I L L I L L 1 1, 27.5 %Control with 3rd Broods: 100
Control Reproduction CV: 6.8
Effluent Percentage 56.4%
48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Control: I 0 of
12
Number of Young Produced 26 31 33 30 27 30 33 31 27 25 31 28 29.3 IWC: o I of 12
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L L -6.7 Significant?: No
%Reducion Final Mortality Significant at: No conc.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 56.4%
"Reduction Homoxedaatic[
Overall Method: '
Effluent Percentage I
Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Method:
I I I I i I I l i I I I I Shapiro-Wilks
Number of Young Produced statistic:
0.942
Adult Survival: (L)ive,(D)ead
Critical Value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage) I Method
F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic:
I(Number of Young Produced I I ill1111111 2 017
( Critical Value: 5.320
Adult Survival: (L)ive,(D)ead
Non-Parametric Analysis(if applicable)
Reduction
Method:
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean 56.4%
Number of Young Produced I I
Adult Survival: (L)ive,(D)ead I I 111111 1
%Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev. 11/95
r
• PO Box 7565
• Asheville,NC 28802 I
Phone: (828)350-9364
= 11_7 , Fax: (828)350-9368
Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: July 29,2019
Facility: Water Quality NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Solutions,I _ Comments:
Signature of Operator in Responsible Charge: Aw_-
Signature of Laboratory Supervisor: Project: 14324
Samples: 190709.13,190711.28
' Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 07-10-19 07-17-19 1254 0801
Sample Information Sample I Sample 2 Control
Collection start date: 07-08-19 07-10-19 ,I Test Information start Renewal I Renewal 2 Start Renewal 1 Renewal
Grab: . s}.. Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24 h 24-hs .i� Initial pH(SU): 7.78 7.70 7.88 7.63 7.61 7.65
Alkalinity(mg/L CaCO3): - to tW a,, 32,32 FinalpH SU: 7.80 7.95 7.90 7.50 7.66 7.60 1
..cW ( )
Hardness(mg/L CaCO3): LW:4j 44 46 Initial DO(mg/L): 8.0 8.1 8.1 8.0 8.1 7.7
Conductivity(pmhos/cm): 409 430 160.158.164 Final DO(mg/L): 8.2 8.2 8.2 8.0 7.9 7.8
Total residual chlorine(mg/L): <0.10 <0.10 Initial Temp.CC): 24.8 24.8 25.0 24.6 24.7 24.8
Sample Temp.at Receipt CC): 1.4 0.9 r4 Final Temp.(°C):. 25.1 25.0 24.7 25.0 25.1 24.8
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Chronic Test Results
Number of Young Produced 34 29 28 28 28 28 28 29 30 30 27 28 28.9 ' Final Control Mortality(%): 0.0
Adult Survival: (L)ive,(D)ead L L L L L L L L L L . L L %Control with 3rd Broods: 100
Control Reproduction CV: 6.3
Effluent Percentage 56.4% 48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Control: 0 of 12
Number of Young Produced 32 29 34 31 29 29 28 31 33 32 33 34 31.3 IWC: 0 of 12
Adult Survival: (L)ive,(D)ead L L L L L I. L L L L L L -8.1 Significant?: No
R0dOL11O° Final Mortality Significant at' No cone.
Effluent Percentage
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Reproduction Analyses
Number of Young Produced Reproduction LOEC: >56.4%
Adult Survival: (L)ive,(D)ead Reproduction NOEC: 56.4%
%.Reduction
Overall Method: Homuscedastic t
Effluent Percentage Normal Distribution: Yes
Treatment 4 Organisms I 2 3 4 5 6 7 8 9 10 11 12 Mean Method: Shapiro-Wilk's
1 Number of Young Produced Statistic: 0.956
Adult Survival: (L)ive,(D)ead Critical value: 0.884
Reduction
Equal Variances: Yes
Effluent Percentage Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Statistic: 1.307
Number of Young Produced Critical Value: 5.320 '
Adult Survival: (L)ive,(D)ead Non-Parametric Analysis(if applicable)
%Reduction
Method:
Effluent Percentage Effluent% Rank Sum Critical Sum
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean 56.4%
Number of Young Produced
Adult Survival: (L)ive,(D)ead
�. %Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4%
DWQ form AT-3(8/91)Rev. 11/95
•
PO Box 7565
•
III
Asheville,NC 28802
Phone: (828)350-9364
/ ., Fax: (828)350-9368
E-mail: Jim@etsnclab.com
Environmental Testing Solutions,Inc.
Date: July 26,2019
Effluent Aquatic Toxicity
Report
Form_
Chronic Fathead Minnow Multi-Concentration Test
Facility: Water Quality Lab&Operations,Inc. NPDES#: NC- 0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Solution ,Inc Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor: C Project: 14309
Samples: 190708.01,190710.24,190712.15
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 27699-1621 07-09-19 07-16-19 0907 0819
Replicate number
1 2 3 4 Test Organisms
Control Surviving number of larvae 10 10 10 10 Survival(%) 100.0 Source:
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.787 In-house Culture
Weight/original(mg/larvae) 0.763 0.798 0.782 0.806 Average wt/ 0.787
surviving(mg) Begin hatch: 07-08-19 1700
Effluent Surviving number of larvae 10 10 10 10 End hatch: 07-09-19 0605
14% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.710 0.750 0.726 0.797 Average wt(mg) 0.746
%Effluent Surviving number of larvae 10 10 10 10
28% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.765 0.695 0.849 , 0.772 Average wt(mg) 0.770
%Effluent Surviving number of larvae 10 10 10 10
56.4% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.728 0.756 0.815 0.719 Average wt(mg) 0.755
\__- %Effluent Surviving number of larvae 10 10 10 10
78% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.739 0.699 0.723 0.720 Average wt(mg) 0.720
Effluent Surviving number of larvae 9 9 10 10
l00% Original number of larvae 10 10 10 10 Survival(%) 95.0
Weight/original(mg/larvae) 0.673 0.681 0.741 0.786 Average wt(mg) 0.720
Water Quality Data
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6
Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final
Control
pH(SU): 7.65 7.56 7.63 7.47 7.64 7.43 7.61 7.44 7.59 7.43 7.52 7.52 7.65 7.60
DO(mg/L): 7.8 7.6 8.0 7.6 8.0 8.0 8.1 7.3 7.6 7.3 7.5 7.3 7.7 7.6
Temp.(CC): - 24.7 24.6 24.7 24.4 24.8 24.4 24.7 24.3 24.7 24.6 24.8 24.5 24.7 24.2
High Concentration
pH(SU): 7.96 7.74 7.92 7.74 7.91 7.71 7.90 7.77 7.82 7.41 7.80 7.66 7.85 7.73
DO(mg/L): 8.0 7.7 8.2 7.7 8.1 7.6 8.3 7.0 7.8 7.0 7.6 7.2 7.9 7.5
Temp.CC): 24.8 24.5 24.9 24.6 25.0 24.5 24.9 24.0 24.8 24.5 24.6 24.6 24.6 24.4
Sample Information Sample 1 Sample 2 Sample 3 Control Analyses Survival Growth
Collection start date: 07-07-19 07-09-19 07-11-19 Normal: Yes Yes
Grab: -
Horn.Var. Yes Yes
Composite duration: 24-h 24-h 24-h NOEC: 100% _ 100%
Alkalinity(mg/L CaCO3): 66 76 50 32,32 LOEC: >100% >100%
Hardness(mg/L CaCO,): 87 95 71 46,44 ChV: >100% >100%
Conductivity(µmhos/cm): 327 412 262 156-165 Method: Visual Insp. Dunnett's
Total residual chlorine(mg/L): <0.10 <0.10 <0.10
Sample Temp.at Receipt CC): 1.1 1.9 1.2 Survival Growth
%Effluent Critical Calculated Critical Calculated
14% 2.410 1.386
28% 2.410 0.568
56.4% 2.410 1.094
78% 2.410 2.238
Overall Analysis: 100% 2.410 2.238
Result: PASS LOEC: >100% NOEC: 100% ChV: >100%
DWQ form AT-5(8/03)
L
•
• PO Box 7565
Asheville,NC 28802
t
Phone: (828)350-9364
'� Fax (828)350-9368
..r` Environmental Testing Solutions,Inc.
Effluent Aquatic Toxicity Report Form-Phase II Chronic Ceriodaphnia dubia Date: October 25,2019
Facility: Water Quality NPDES#: NC-0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testin Solutions,Inc. Comments:
Signature of Operator in Responsible Charge:
Signature of Laboratory Supervisor:
Project: 14566
Samples: 191008.22,191010.27
Mail Original To:North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date End date: Start time: End time: I
Raleigh,NC 27699-1621 I 10-09-19 1 10-16-19 1323 0842
Sample Information Sample 1 Sample 2 Control
Collection start date: 10-07-19 10-09-19 Test Information start Renewal I Renewal i sun Renewal I Renewal 2
Grab: Treatment: 56.4% 56.4% 56.4% Control Control Control
Composite duration: 24-h 24-h Initial pH(SU): 7.97 8.00 8.06 7.89 7.86 7.89
Alkalinity(mg/L CaCO3): - 62,60,59 Final pH(SU): 8.00 8.09 8.10 7.81 7.96 7.96
Hardness(mg/L CaCO3): k_ ��- 90 m 94,88 Initial DO
(mg/L): 8.2 8.2 8.1 7.8 7.8 7.9
Conductivity(µmhos/cm): 456 488 306.307.320 Final DO m
( W-): 8.3 8.3 8.1 7.9 7.8 7.9
Total residual chlorine(mg/L): <0.10 <0 10 Initial Tetu . C
£r P (e ) 24.9 25.0 24.8 24.9 24.9 24.7
Sample Temp.at Receipt CC): 2.2 1.1 - Final. r. _. � Temp.(°C): 25.0 25.0 25.1 24.9 25.0 25.1
Organism Number
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Me.,
!Number of Young Produced I 31 I 28 28 I 291 271 27 i 281 29 I 271 26 I 30 28 Chronic Test Results
Adult Survival: (L)ive,(D)ead L L L L L L L L L L L 1 L 1 Final Contro llM28.2 I Mortality(%); 0 0
%Control with 3rd Broods: 100
Control Reproduction CV: 5.0 '
Effluent Percentage 56.4%
48 Hour Mortality
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean Control: 0 of
(Number of Young Produced 34 I 321 351 38 37 30 35 35 31 37 28 31 33.6 IwC: I 0 I of 12
12
Adult Survival: (L)ive,(D)ead I L L L L I L I L I L 1 L 1 L I L ( L I L I -19.2 i
Significant?: No
%Reduction
Final Mortality Significant at No coot.
Effluent Percentage) I
Treatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean
I I I I 1 1 1 I 1 I 1 I 1 Reproduction LOEC:
>56.4 Reproduction Analyses
Number of Young Produced 1
Adult Survival: (L)ive,(D)ead
Reproduction NOEC: 56.4%
v.Reducion Overall Method:
Homoscedastic t
Effluent Percentage I
Normal Distribution: Yes
Treatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mea. Method
(Number of Young Produced sn.piro-wiik s
Statistic: 0.984
Adult Survival (L)ive,(D)ead 1 1 I l I
I l l I
I
I
I
Critical Value: 0.884
%Reduction
Equal Variances: Yes
Effluent Percentage
Method: F-Test
Treatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 me. Statistic:
(Number of Young Produced 5.027
Adult Survival: (L)ive,(D)ead I I I I 1 1
I I I I I I I Critical Value: 5.320
Non-Parametric Analysis(if applicable)
%Reduction
Method:
Effluent Percentage
Treatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mqffl
E 56.4%/ Sum Critical Sum
Number of Young Produced I I I l I I 1 1 1 I 1
Adult Survival: (L)ive,(D)ead I I%Reduction
Overall Analysis:
Result: PASS LOEC: >56.4% NOEC: 56.4% ChV: >56.4% t
DWQ form AT-3(8/91)Rev. 11/95
s PO Box 7565
Asheville,NC 28802
0
Phone: (828)350-9364
a Fax: (828)350-9368
E-mail: Jim@etsnclab.corn
Environmental Testing Solutions,Inc. Date: October 25,2019
Effluent Aquatic Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test
Facility: Water Quality Lab&Operations,Inc. NPDES#: NC- 0020451 Pipe#: 001 County: Ashe
. West.Jefferson WW'I"P
Laboratory Performing Test: Environmental Testin lutions,�Inncc .iroc Comments:
Signature of Operator in Responsible Charge: 4.,
Signature of Laboratory Supervisor: t ILI Project: 14539
�J Samples: 191007.01,191009.26,191011.15
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh,NC 2 7699-1 62 1 10-08-19 10-15-19 0818 0739
Replicate number
1 2 3 4 Test Organisms
Control Surviving number of larvae 10 10 10 10 Survival(%) 100.0 Source:
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.794 ( In-house Culture
Weight/original(mg/larvae) 0.757 0.793 0.737 0.890 Average wt/ 0.794
surviving(mg) Begin hatch: 10-07-19 1500
%Effluent Surviving number of larvae 10 10 10 10 End hatch: 10-08-19 0510
14% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.750 0.844 0.900 0.805 Average wt(mg) 0.825
Effluent Surviving number of larvae 10 10 10 10
28% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.776 0.820 0.794 0.838 Average wt(mg) 0.807
%Effluent Surviving number of larvae 10 10 10 10
56.4% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.805 0.785 0.783 0.807 Average wt(mg) 0.795
Effluent Surviving number of larvae 10 10 10 10
78% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.775 0.752 0.842 0.816 Average wt(mg) 0.796
Effluent Surviving number of larvae 10 10 10 10
100% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.727 0.852 0.956 0.944 Average wt(mg) 0.870
Water Quality Data
Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6
Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final
Control
pH(SU): 7.99 7.80 7.89 7.77 7.87 7.75 7.86 7.76 7.81 7.94 8.00 7.80 7.89 7.89
DO(mg/L): 7.6 7.7 7.8 7.6 7.8 7.8 7.8 8.2 7.7 8.0 7.8 7.8 7.9 7.6
Temp.CC): 24.8 24.8 24.7 24.9 _ 24.7 25.2 24.9 24.8 24.8 25.0 24.8 25.1 24.7 25.0
High Concentration
PH(SU): 8.14 7.90 8.04 7.96 8.09 7.90 8.08 7.86 8.20 8.04 8.26 8.02 8.21 8.10
DO(mg/L): 8.1 7.8 7.9 7.7 8.1 7.8 8.0 8.1 8.3 8.1 8.0 7.7 8.0 7.8
Temp.CC): 25.0 24.8 24.9 25.0 25.0 25.1 25.0 25.1 25.0 24.9 25.0 24.9 24.8 24.7
Sample Information Sample 1 Sample 2 Sample 3 Control Analyses Survival Growth
Collection start date: 10-06-19 10-08-19 10-10-19 Normal: Yes Yes
Grab: . Horn.Var. Yes Yes
Composite duration: 24-h 24-h 24-h NOEC: 100% 100%
Alkalinity(mg/L CaCO3): 88 90 96 59-62 LOEC: >100% >100%
Hardness(mg/LCaCO3): 98 98 96 88-94 ChV: >100% >100%
Conductivity(µmhos/cm): 459 461 521 301-320 Method: Visual Insp. Dunnett's
Total residual chlorine(mg/L): <0.10 <0.10 <0.10
•
Sample Temp.at Receipt(°C): 2.6 1.1 2.2 Survival Growth
%Effluent Critical Calculated Critical Calculated
14% . . 2.410 -0.706
28% . 2.410 -0.295
56.4% 2.410 -0.017
78% 2.410 -0.046
Overall Analysis: t00% 2.410 -1.748
Result: PASS LOEC: >100% NOEC: 100% ChV: >100%
DWQ form AT-5(8/O3)
I
PO Box 7565
a Asheville,NC 28802
V Phone: (828)350-9364
p}
.- = Fax: (828)350-9368
)EnsironmentalT ang Solutions,Inc.
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date: January 24,2020
Inc. NPDES#: NC0020451 Pipe#: 001 County: Ashe
Facility: Water Quality Lab&Operations, cy
West Jefferson WWTP
Laboratory Performing Test: Environmental Testing Solutions,Inc.,Certificate#037 Comments:
Signature of Operator in Responsible Charge(ORC):
ORC Phone/E-mail: W ,CV pekk_ Project#: 14740
Signature of Laboratory SuperG isttf: ( Sample#: 200106.02,200108.30,200110.20
e-Mail to: ATForms.ATB@ncdenr.gov Test Organisms: Pimephales promelas
Or Mail Original to: North Carolina Division of Water Resources Supplier
Water Sciences Section/Aquatic Toxicology Branch In-house Culture
' 1621 Mail Service Center Begin hatch: 01-06-20 1325
Raleigh,NC 27699-1621 End hatch: 01-07-20 0610
Test Start Date: January 07,2020
Replicate number
1 2 3 4
Control Surviving number of larvae 10 10 10 10 Survival(%) 100.0
Organisms Original number of larvae 10 10 10 10 Average wt(mg) 0.730
Weight/original(mg/larvae) 0.764 0.716 0.686 0.755 Averagewt/ 0.730
surviving(mg)
%Effluent Surviving number of larvae 10 10 10 10
14% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.729 0.677 0.727 0.716 Average wt(mg) 0.712
%Effluent Surviving number of larvae 10 10 10 10
28% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.703 0.816 0.669 0.718 Average wt(mg) 0.727
%Effluent Surviving number of larvae 10 10 10 10
56.4% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.677 0.722 0.646 0.645 Average wt(mg) 0.673
%Effluent Surviving number of larvae 10 10 10 10
78% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.683 0.638 0.720 0.766 Average wt(mg) 0.702
%Effluent Surviving number of larvae 10 10 10 10
100% Original number of larvae 10 10 10 10 Survival(%) 100.0
Weight/original(mg/larvae) 0.682 0.671 0.603 0.689 Average wt(mg) 0.661
Water Quality Data
Day 0 Day 1 Day 2 Day 3 Day 4 Day S Day 6
Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final Initial I Final
Control
pH(SU): 7.83 7.75 7.73 7.75 7.93 7.67 7.73 7.74 7.89 7.66 7.94 7.68 7.83 7.75
DO(mg/L): 7.8 8.0 7.9 7.8 7.8 7.4 7.8 7.8 7.7 7.9 7.7 7.5 7.8 7.9
Temp.("C): 24.8 25.0 24.7 24.9 24.8 24.8 24.8 24.7 24.8 25.1 24.9 24.9 24.8 24.8
High Concentration
pH(SU): 8.14 7.99 8.12 7.93 8.07 7.81 8.11 7.86 8.00 7.87 8.13 7.83 8.13 7.93
DO(mg/L): 8.1 7.8 8.0 7.9 8.2 7.6 8.1 7.8 8.1 8.0 8.1 75 8.2 7.9
Temp.(°C): 25.0 24.9 24.8 24.7 25.0 25.0 24.7 24.9 24.7 24.9 25.0 24.8 25.0 25.1
Sample Information Sample 1 Sample 2 Sample 3 Control Analyses Survival Growth
Collection start date: 01-05-20 01-07-20 01-09-20 Normal: Yes Yes
Grab: - Horn.Var. Yes Yes
Composite duration: 24-h 24-h 24-h NOEC: 100% 100%
Alkalinity(mg/L CaCO3): 96 88 92 59-61 LOEC: >100% >100%
Hardness(mg/LCaCO3): 100 100, 100 88-90 ChV: >100% >100%
Conductivity(µmhos/cm): 454 483 513 301-318 Method: Visual Insp. Dunnett's
Total residual chlorine(mg/L): <0.10 <0.10 <0.10
Sample Temp.at Receipt("C): 1.5 1.9 2.2 Survival Growth
%Effluent Critical Calculated Critical Calculated
14% . 2.410 0.576
28% . . 2.410 0.120
56.4% 2.410 1.849
78% 2.410 0.913
Overall Analysis: 100% 2.410 2.210
Result: PASS LOEC: >100% NOEC: 100% ChV: >100%
DWR Report Form AT-5
F
• PO Box 7565
Asheville, NC 28802
Phone: (828)350-9364
,.. Environmental Testing Solutions,inc. Fax: (828)350-9368
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: January 24, 2020
Facility: Water Quality Lab&Operations, Inc. NPDES#: NC 0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testing Solutions, Inc., C rtificate#037 Comments:
Signature of Operator in Responsible Charge(ORC):
ORC Phone/E-mail: . Project#: 14766
Signature of Laboratory Supervisor: Sample#: 200107.24,200109.21
e-Mail to: ATForms.ATB@ncdenr.Rov
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-Stat/Rank Sum -4.132
Raleigh,NC 27699-1621 1-Tailed Critical 2.508
%Reduction: -14.1
Percent Average
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Mortality Reproduction
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Control Control
Number of Young Produced 28 29 27 31 32 29 33 30 30 34 33 33 8.3 30.8
Adult Survival: (L)ive, (D)ead L L D L L L L L L L L L Treatment 2 Treatment 2
0.0 35.1
Effluent Percentage 56.4% Control CV
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 7.4 PASS FAIL
Number of Young Produced 37 37 36 35 28 35 38 37 37 33 36 32 %control organisms producing
3rd brood
Adult Survival: (L)ive, (D)ead L L L L L L L L L L L L 100.0
pH(S.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date: January 08, 2020
Control 7.73 7.85 7.73 7.95 7.83 7.90
Treatment 2 7.98 8.01 7.90 8.07 7.97 8.02 Collection (Start) Date:
- Sample 1 01-06-20 Sample 2 01-08-20
:s :° m
W w w
D.O.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control 7.9 8.0 7.8 7.9 7.8 7.8 Grab Comp. Duration
Treatment 2 8.2 8.2 8.1 8.2 8.1 8.3 Sample 1 . X 24-h = r+ r
Sample 2 . X 24-h a a
p 3 E
E
to v)
LC50/Acute Toxicity Test Alkalinity(mg CaCO3/L) 61,60,59 NW, A,
(Mortality expressed as%, combining replicates.) Hardness(mg Cac03/L) 90,90,88 ' '
Conductivity(µmhos/cm) 316,317,301 471 536
Concentration(%) Total Residual Chlorine(mg/L); ej <0.10 <0.10
Mortality(%) Sample Temp.at Receipt C
LC50= Method of Determination
95%Confidence Limits Trimmed Spearman Karber t -0 t: -
nzi _
to Probit WI V '
Other: Control
High Conc.
Organism Tested: Duration: pH (S.U.) DO(mg/L)
DWR Report Form AT-1
PO Box 7565
Asheville,NC 28802
Phone: (828)350-9364
Environmental Testing Solutions,Int.
Fax: (828)350-9368
Effluent Toxicity Report Form-Chronic Pass/Fail and Acute LC50 Date: April 24,2020
Facility: Water Quality Lab&Operations,Inc. NPDES#: NC 0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
Laboratory Performing Test: Environmental Testing Solutions,Inc.,Certificate#037 Comments:
Signature of Operator in Responsible Charge(CRC): .--'
ORC Phone/E-mail:Wwtp@townofWj.CO Project#: 15004
Signature of Laboratory Supervisor: Sample 4: 200407.28,200409.26
e-Mail to: ATForms.ATB(aincd nr.tzov
Or Ma+l Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-5tat/Rank Sum -7.917
Raleigh,NC 27699-1621 1-Tailed Critical 2.508
%Reduction: -19.4
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent Average
Mortality Reproduction
Control Organisms 1 2 3 4 5 b 7 8 9 10 11 12 Control Control
Number of Young Produced 32 30 29 30 29 27 29 30 31 31 33 29 0.0 30.0
Adult Survival: (L)ive,(D)ead L L LLLLL ILLL L Treatment 2 Treatment 2
0.0 35.8
Effluent Percentage 156.4%i Control CV
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 5.3 PASS FAIL
Number of Young Produced 36 38 36 34 33 35 38 35 34 34 38 39 %-p`••37,7,7,, rodit"°
Adult Survival: (L)ive,(D)ead LLL LLLLILLIL 100.0 •
pH(S.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date: April 08, 2020
Control 7.74 7.83 7.83 7.92 7.82 7.76
Treatment 2 7.51 7.68 7.65 7.72 7.65 7.78 Collection(Start)Date:
U,
t v Sample 1 04-06-20 Sample 2 04-08-20
N W N W
D.O.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control 7.8 7.9 7.8 8.0 7.8 17.8 I Grab Comp. Duration
Treatment T 8.1 8.1 8.0 8.3 8.1 8.2 Sample 1 X 24-h ml
Sample 2 X 24-li 2 m a a
5 R i
LC50/Acute Toxicity Test Alkalinity(mg CaCO3/L) 60,60,61
(Mortality expressed as%,combining replicates.) Hardness(mg CaCO3/L) 89,89,87 >.
U � Asa. .1!
Conductivity(µmhos/cm) 315,312,312 432 391
Concentration(%) ) Total Residual Chlorine(mg/L) V h e <0.10 <0.10
I
I
Mortality(%) Sample Temp.at Receipt 1°C) 2.9 1.8
LC50= Method of Determination
95%Confidence Limits Trimmed Spearman Karber
a to Probit W
Other: I Control
I High Conc. 1 1
Organism Tested: Duration: pH(S.U.) DO(mg/L)
DWR Report Form AT-1
k:'' :g'` PO Box 7565
Asheville,NC 28802
Phone: (828)350-9364
Environmental imernil tmlutions.toe-
Fax: (828)350-9368
Effluent Toxicity Report Form-Chronic Pass/Fail and Acute LC50 Date: July 31,2020
Facility: Water Quality Lab&Operations,Inc.NPDES#: NC 0020451 Pipe#: 001 County: Ashe
West Jefferson WWTP
laboratory Performing Test: Environmental Testing Solutions,Inc. C ' • at #03 Comments
Signature of Operator in Responsible Charge(ORC):
ORC Phone/E-mail: 47.5(Aa/1 ' QdQ/yy Project#: 15230
Signature of Laboratory Supery or Sample#: 200707.27,200709.25
e-Mail to: ATForms.ATB@ncdenr.gov
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch Chronic Test Results
1621 Mail Service Center t-Stat/Rank Sum -3.738
Raleigh,NC 27699-1621 1-Tailed Critical 2.508
%Reduction: -10.3
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Percent Average
Mortality Reproduction
Control Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Control Control
Number of Young Produced 28 27 26 28 25 30 27 27 29 27 29 28 0.0 27.6
Adult Survival: (l)ive,(D)ead L L L L L L L L L L L L Treatment 2 Treatment 2
0.0 30.4
Effluent Percentage 56.4% Control CV
Treatment 2 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 5.0 PASS FAIL
Number of Young Produced 34 28 29 33 28 29 32 30 29 33 32 28 %"'"" ""°""°""""`
7rA S and
Adult Survival: (L)ive,(D)ead I L L L L L L L L L L L 100.0 X '
pH(S.U.) 1st Sample 2nd Sample 2nd Sample Test Start Date: July 08,2020
Control 7.78 7.80 7.56 7.92 8.02 7.79
Treatment 2 7.78 7.86 8.16 8.16 7,91 7.90 Collection(Start)Date:
- t -, e -, Sample 1 07-06-20 Sample 2 07-08-20
c
N w
D.O.(mg/L) 1st Sample 2nd Sample 2nd Sample Sample Type/Duration
Control 7.8 7.9 7.8 7.9 7.8 7.9 Grab Comp. Duration
Treatment 2 8.0 8.2 7.8 8.2 7.9 8.1 Sample 1 • X 24-h g t. N
Sample 2 • X 24-h 6 E o
r n ION
LCso/Acute Toxicity Test Alkalinity(mg CaCO3/1) 60,61,60 e 2 '
(Mortality expressed as%,combining replicates.) Hardness(mg CaCO3/L) 87,89,85 `' �
Conductivity(limbos/cm) 316,3og,312 309 399
Concentration(%) ..;#,r:;
Total Residual Chlorine(mg/L) i, <0.10 <0.10
Mortality(%) Sample Temp.at Receipt(°C) ` 1.2 2.7
LC50= Method of Determination
95%Confidence Limits Trimmed Spearman Karber r -o r
-
to Probit W L,, 1
Other: Control
High Conc.
Organism Tested: Duration: pH(S.U.) DO(mg/L) 1
DWR Report Form AT-1
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 10/15/20
Facility: WEST JEFFERSON NPDES#: NC0020451 Pipe#: 001 County: ASHE
Laborat y Per orm' a ECH LABS, INC.
Comments:
X
i re p r toz i, Responsib `e`Charge
Signature of aboratory Supervisor * PASSED: -1.71W Reduction *
Work Order: Environmental Sciences Branch
MAIL ORIGINAL TO: Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t = -0.482
Tabular t = 2.508
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Reduction = -1.71
% Mortality Avg.Reprod.
# Young Produced 26 23 24 24 26 21 23 23 25 27 24 27
0.00 24.42
Adult (L)ive (D)ead L L L L L L L L L L L L Control Control
0.00 24.83
Treatment 2 Treatment 2
Effluent %: 56%
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Control CV
7.503o PASS FAIL
# Young Produced 28 24 25 20 26 23 25 23 26 24 25 29i % control orgy X
- producing 3rd
brood Check One
Adult (L)ive (D)ead L L L L L L L L L L L L 100%.
1st sample 1st sample 2nd sample Complete This For Either Test
pH Test Start Date: 10/07/20
Control 8.03 7.93 7.88 7.94 7.94 7.93 Collection (Start) Date
Sample 1: 10/05/20 Sample 2: 10/07/20
Treatment 2 7.95 7.98 7.82 8.02 7.89 8.01 Sample Type/Duration 2nd
1st P/F
s s s Grab Comp. Duration D
t e t e t e I S S 1
a n a n a n Sample 1 X 24.0 hrs L A A
r d r d r d U M M
t t t Sample 2 X 24.0 hrs T P P
1st sample 1st sample 2nd sample
D.O. Hardness(mg/1) 44
Control 7.91 7.73 7.93 7.46 7.78 7.64
Spec. Cond. (pmhos) 155 256 272
Treatment 2 7.93 7.86 8.08 7.64 7.77 7.74
Chlorine(mg/1) <0.1 <0.1
LC50/Acute Toxicity Test Sample temp. at receipt(0C) 0.8 0.8
(Mortality expressed as %, combining replicates) )
Note: Please
W % % % % % % t % % Concentration Complete This
Section Also
t % % % % % % % % t Mortality
start/end start/end
LC50 = % Method of Determination Control
95% Confidence Limits Moving Average Probit
* -- % Spearman Kerber - Other - High
Conc.
pH D.O.
Organism Tested: Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT-1 (3/87) rev. 11/95 (DUBIA ver. 4.41)
PO Box 228 • Statesville, NC 28687 • 704/872/4697
Page 3 of 9
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 01/14/21
Facility: WEST JEFFERSON NPDES#: NC0020451 Pipe#: 001 County: ASHE
Labor e y Perf rming est: MER CH LABS, INC.
X "lit_n a* Comments:
nature of Qper t in esponsible Charge
r 7'","
Signature of Laboratory Supervisor * PASSED: -9.06% Reduction *
Work Order: Environmental Sciences Branch
MAIL ORIGINAL TO: Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t = -2.241
Tabular t = 2.508
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Reduction = -9.06
% Mortality Avg.Reprod.
# Young Produced 18 23 21 17 23 20 21 23 21 21 24 22
0.00 21.17
Control Control
Adult (L)ive (D)ead L L L L L L L L L L L L
0.00 23 .08
Treatment 2 Treatment 2
Effluent %: 56%
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Control CV
9.835% PASS FAIL
# Young Produced 22 23 23 24 27 24 22 26 22 20 24 20 % control orgs X
producing 3rd
brood Check One
Adult (L)ive (D)ead L L L L L L L L L L L L 100%
1st sample 1st sample 2nd sample Complete This For Either Test
pH Test Start Date: 01/06/21
Control 8.07 7.84 8.16 7.88 8.37 7.95 Collection (Start) Date
Sample 1: 01/04/21 Sample 2: 01/02/21
Treatment 2 7.62 8.07 7.69 8.05 7.88 8.06 Sample Type/Duration 2nd
1st P/F
s s s Grab Comp. Duration D
t e t e t e I S S
a n a n a n Sample 1 X 24 hrs L A A
r d r d r d U M M
t t t Sample 2 X 24 hrs T P P
1st sample 1st sample 2nd sample
D.O. Hardness(mg/1) 46
Control 8.05 7.53 7.98 7.89 8.32 8.09
Spec. Cond. (pmhos) 167 301 296
Treatment 2 8.02 7.87 8.20 8.00 8.31 8.19
Chlorine(mg/1) <0.1 <0.1
LC50/Acute Toxicity Test Sample temp. at receipt(°C) 0.4 0.5
(Mortality expressed as %, combining replicates) I
Note: Please
% % % % % % % % % % Concentration Complete This
Section Also
% % % % % % % % % % Mortality
start/end start/end
LC50 = % Method of Determination Control
95% Confidence Limits Moving Average _ Probit
% -- % Spearman Karber _ Other High
Conc.
pH D.O.
Organism Tested: Ceriodaphnia dubia Duration(hrs) :
Copied from DWQ form AT-1 (3/87) rev. 11/95 (DUBIA ver. 4.41)
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 04/14/21
Facility: WEST JEFFERSON NPDES#: NC0020451 Pipe : 001 County: ASHE
Labo ory ' T ME TECH LABS., INC.
Comments:
X
{ ya�ture of 0 rator in esponsible Charge • '
X U"/c
ignatu e 0 L ora ory rvisor * PASSED.: 6.15% Reduction *
Work Order: Environmental Sciences Branch
MAIL ORIGINAL TO: Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t = 0.816
Tabular t = 2.508
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 & Reduction = 6.15
% Mortality Avg.Reprod,
# Young Produced 23' 18 23 21 13 19 25 16 23 16 23 24
0.00 20.33
Control Control
Adult (L)-ive (D)ead L L L L L L L .L L L L L
8.33 19..08
Treatment 2 Treatment 2
Effluent %: 56%
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Control CV
18.912% PASS FAIL
# Young Produced 16 21 10 20 22 21 20 20 23 21 20 15 % control orgs X
producing 3rd
Adult (Wive (D)ead L L L L D L L L L L L L brood100% Check One
1st sample 1st sample 2nd sample Complete This For Either Test
pH Test Start Dater 04/07/21
Control 7.99 7.90 7.9'0 8.05 7.79 7.98 Collection (Start) Date
Sample 1: 04/05/21 Sample 2: 04/07/21
Treatment 2 7.58 8.00 7.63 8.11 7.80. 8.10 Sample Type/Duration 2nd
1st P/F
s s s Grab Comp. Duration D
t e t e t e I S S
a n a n a n Sample 1 X 24.0 hrs L A A
r d r d r d U M M
t t t Sample 2 X 24.0 hrs T P P
1st sample 1st sample 2nd sample
D.O. Hardness(mg/1) 44
Control 8.12�7.73 7.86 7.42 7.84 7.90
Spec. Cond. (pmhos) 151 443 472
Treatment 2 7.97 7.76 8.03 7.45 7.83W7.88
Chlorine(mg/1) <0.1 <0.1
LC50/Acute Toxicity Test Sample temp. at receipt(°C) 0.5 0..6
(Mortality expressed as %, combining replicates) S
Note: Please
% % % % % % % % % % Concentration Complete This
& It & I & % % & & & Mortality Section Also
start/end start/end
LC50 = I Method of Determination J Control
95% Confidence Limits Moving Average Probit
I -- % Spearman Karber -- Other — High
Conc.
PH A.O.
Organism Tested: Ceriodaphnia dubia Duration(hrs) :
Copied from DWQ form AT-1 (3/87) rev. 11/95 (DUBIA ver. 4.41)
PO B x
•
0 228 • Statesville, NC 28687 • 704/872/4697
Page 3 of 9
Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 07/22/21
Facility: WEST JEFFERSON 11 NPDES#: NC0020451 Pipe#: 001 County: ASHE
Labo i or�for n T st/1���RITECH LABS, INC.
Comments:
X Ar
S' •v. o O r t r n Responsible Charge
X 1
Signature of Laboratory Supervisor * PASSED: 13 .41% Reduction *
I Work Order: Environmental Sciences Branch
MAIL ORIGINAL TO: Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t = 4.270
Tabular t = 2.508
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Reduction = 13.41
% Mortality Avg.Reprod.
# Young Produced 26 30 28 28 26 26 28 30 29 30 22 25
0.00 27.33
Control Control
Adult (L)ive (D)ead L L L L L L L L L L L L
0.00 23 .67
Treatment 2 Treatment 2
Effluent %: 56%
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Control CV
8.871% PASS FAIL
# Young Produced 21 24 27 25 23 24 25 24 25 22 22 22 % control orgs X
producing 3rd
brood Check One
Adult (L)ive (D)ead L L L L L L L L L L L L 100%
1st sample 1st sample 2nd sample Complete This For Either Test
pH Test Start Date: 07/14/21
Control 8.23 8.07 8.15 8.11 7.90 8.00 Collection (Start) Date
Sample 1: 07/12/21 Sample 2: 07/14/21
Treatment 2 7.79 8.05 7.96 8.30 8.00 8.20 Sample Type/Duration 2nd
1st P/F
s s s Grab Comp. Duration D
t e t e t e I S S
a n a n a n Sample 1 X 24.0 hrs L A A
✓ d r d r d U M M
t t t Sample 2 X 24.0 hrs T P P
1st sample 1st sample 2nd sample
D.O. Hardness(mg/1) 48
Control 8.15 7.59 8.02 7.88 8.04 7.59
Spec. Cond. (pmhos) 165 226 488
Treatment 2 8.06 7.60 8.11 7.84 8.01 7.65
Chlorine(mg/1) <0.1 <0.1
LC50/Acute Toxicity Test Sample temp. at receipt(°C) 1.1 1.5
(Mortality expressed as %, combining replicates)
Note: Please
% % % % % % % % % % Concentration Complete This
Section Also
% % % % % % % % % % Mortality
start/end start/end
LC50 = % Method of Determination Control
95% Confidence Limits Moving Average Probit
% -- % Spearman Karber = Other - High
Conc.
pH D.O.
Organism Tested: Ceriodaphnia dubia Duration(hrs) :
Copied from DWQ form AT-1 (3/87) rev. 11/95 (DUBIA ver. 4 .41)
Tom Hartman,Mayor ��'i FFF Russell W.Barr, Ill,Alderman
Y E. 9�` ,
Brantley Price,Town Manager wfr#414?7414)-4,/
' Calvin Green,Alderman
Bradley Jordan,Chief of Police ��, Crystal C.Miller,Alderman
John B."Jak"Reeves,Town Attorney itoc John K. Reeves,Alderman
''NCC ,,,5r Stephen Shoemaker,Alderman
Town of West Jefferson
ESta6Ctshei1915
"Prosperity, Growth,Achievement"
ASHE COUNTY CHEESE
Ashe County Cheese is the holder of Permit number 0003, issued by the Town of
West Jefferson on 12/02/19. The information on the following pages depict the
monitoring requirements of their Permit.
During the period January through December 2020, Ashe County Cheese had the
following violations of their Permit.
DATE SAMPLE PARAMETER MEASURED PERMIT
TYPE VALUE LIMIT
08/11/20 I BOD 195 lbs 100 lbs
Ashe County Cheese regained compliance through re-sampling. Therefore, Ashe County
Cheese was compliant for the monitoring period January—December 2020
Tom Hartman,Mayor �X� . F9 Russell W.Barr,Ill,Alderman
6 rim
Brantley Price,Town Manager ti, �+ �Q`^ ; Calvin Green,Alderman
Bradley Jordan,Chief of Police r�� 74`- ; Crystal C.Miller,Alderman
John B."Jak"Reeves,Town Attorney irk > John K.Reeves,Alderman
Stephen Shoemaker,Alderman
Town of West Jefferson
cEsta6Cuhed 1915
4'rosperity, growth,Ac(tievement"
The Town of West Jefferson has three dental practices:
Mountain Town Dental
Amato Dentistry and Associates
Timothy M Rector, D.D.S., P.A.
All practices have completed the One-Time Compliance Report for Dental Dischargers
and have sent me a copy of the report.
Pretreatment Annual Report(PAR) PAR covers this calendar year=> 2020
Significant Non-Compliance Report(SNCR) Control Authority=Program=Town Name => West Jefferson
WWTP=Wastewater Treatment Plant,use separate form for each WWTP. WWTP Name => West Jefferson WWTP
SIU=Significant Industrial User NPDES# => NC0020451
SNC=Significant Non-Compliance
A SNCR Form must be submitted with every PAR,please write"None"if you had No SIUs in SNC during calendar year
SNC? (Yes/No)
1UP Pipe Industry Name Parameter for each 6-month period.
# # or"Reporting" Jan.-June July-Dec.
NONE
f
Attach a copy of the Division's"SIUs in SNC Historical Report"for your POTW's Sills behind this page .
Is the database correct? Notify the Division of any errors !Database indicates SNC history for previous years.
EVERY SNC MUST be explained in the Narrative, How was,is,or will it be resolved?
REPEAT SNCs are serious matters that MUST be explained in the Narrative.
Form name: PAR,SNCR,2001
Date Revised: 1/4/2001
Chapter 9, PAR Guidance
Pretreatment Performance Summary (PPS)
1. Pretreatment Town Name West Jefferson
2. "Primary"NPDES Number NC0020451 Tfororcce"
e o
or Non_Discharge Permit#if applicable=> usEPAIY
WENDB
3. PAR begin Date,please enter 01/01/yy or 07/01/yy 3.=> 1/1/20 PSSD
4. PAR end Date, please enter 06/30/yy or 12/31/yy 4.=> 12/31/20 PSED
5. Total number of Sills,includes CIUs 5.=> 1 sius
6. Number of CIUs 6.=> 0 cius
7. Enter the Higher Number,either (7a)or(7b)here=> 0 NOIN
7a. Number of SlUs not sampled by POTW 7a.=> 0
7b. Number of Sills not inspected by POTW 7b.=> 0
s. Number of Sills with no IUP,or with an expired IUP 8.=> 0 NOCM
9. Total Number of SlUs in SNC due to either IU Reporting violations or
IUP Limit violations. To avoid double counting some SlUs
Enter 9a+9b -9c here=> 0 PSNC
Enter the Number of Sills in SNC for:
9a. IU Reporting violations 9a.=> 0 MSNC
9b. IUP Limit violations 9b.=> 0 SNPS
9c. Both IU Reporting&Limit violations 9c.=> 0
1o. Number of SlUs in SNC due to IU Reporting violations
that were not sampled or inspected by POTW 10._> 0 SNIN
11. Number of SlUs in SNC due to violation of 11.=> 0 SSNC
Compliance Schedule due dates
12. Number NOVs,NNCs,AOs,or similar assesed to Sills 12.=> 1 FENF
13. Number Civil or Criminal Penalties assessed to Sills 13._> 0 JUDI
14. Number of Sills included in public notice 14.=> 0 SVPU
15. Number of Sills from which penalties collected 15.=> 0 IUPN
is. Total Amount of Civil Penalties Collected 16.=> $ 0
17. Total Number of Sills on a compliance schedule 17.=> 0 socs
Foot Notes: AO Administrative Order NOV Notice of Violation
CIU Categorical Industrial User PAR Pretreatment Annual Report
CO Consent Order POTW Pudic Operated Treatmentt Works
IUP Industrial User Pretreatment Permit SIU Significant Industrial User
NNC Notice of Non-Compliance SNC Significant Non-Compliance,formerly RNC
Chapter name:PAR Guidance File name:PAR_PPS.xls Revision date:August 1,1994 Comprehensive Guide,Chapter 9, Section B, Page 5 Blank PPS Form,Copy and use in your PAR
Pretreatment Program Info Database printed on: 12/22/2020
for Program Name West Jefferson Z IWC%at 7Q10 56.33 \
WWTP Name Town of West Jefferson Stream information
Program Approval Date 11/30/1984 7Q10 Flow cfs/mgd 0.6 / 0.39
1Q10 Flow cfs/mgd 0.51 / 0.33
Pretreatment Status Modified C-TR+
Stream Classification
Region WSRO Basin Number NEW02
County Ashe ` Receiving Stream Name UT LITTLE BUFFALO
NPDES Number NC0020451
NPDES Effective Date Last PAR Rec 02/24/2020 PAR Due Date 03/01/2021 mercury
04/01/2018 1631
NPDES Expire Date Current Fiscal 03/24/2020 required
P 04/30/2022 Year PCI Done
POT is Primary WWTP TRUE Last Audit on 03/12/2019 Audit Year Next23/24 yes
W
Design Flow mgd 0.5000 %Design mgd is SIU permitted 5.00 Permitted SIU flow(mgd)[Pt_SIU) .025
WWTP SIU's 1 Program SIUs 11
WWTP CIU's 0 Program CIUs 0 HWA LTMP MS SUO ERP
date Inactive Date Next Due 04/01/2024 04/01/2024
Date Received by DWR 02/06/2019 03/25/2019 03/25/2019 12/19/2012 02/11/2020
Date Approved 03/04/2019 07/11/2019 07/11/2019 01/10/2013 02/13/2020
Adopt Date Required
Date Adopted 02/04/2013
Info in this Box from Pt Contacts
Date Date Date
PT Pro Attended Attended Attended
Formal Name g.Prime Phonel ext Fax HWA Wksp IUP Wksp PAR Wksp
Mr.Brantley Price I 11(336)246-3551 1(336)246-4409 II
Town Manager IPO Box 490 28694
Mr.Brandon Patrick IPrim 11336-977-6725 I 1336-246-4409 II I I I
wwtp@townofwj.com Pretreatment Coordinator IPO Box 490 28694
SPretreatment Related NOVs from DWQ i \ DWR Central Office Contact Vivien Zhong
5/28/2002 enforce,hwa,sample(pci) DWR Regional Contact Jim Gonsiewski
6/11/2004 enforcement-hg+cd passthru(audit)