NC0037508_Toxicity_20100929

Lopez, Dale
From: Lopez, Dale
Sent: Wednesday, September 29, 2010 9:59 AM
To: Henson, Belinda; Brantley, Mark; Rawls, Paul; White, Hughie; Allen, Trent; Danny Strickland;
Lawyer, Mike
Cc: Lopez, Dale; Dana Folley
Subject: Moore County Regional POTW
Janna Scherer reported that the September quarterly WET resulted in a "Fail". She will begin her search for the cause,
starting within the POTW, then to the collection system, and the ClUs. Luckily, she will also have a lot of lab data on the
influent and effluent because she did her LTMP during the same week.
1

Lopez, Dale
From: Scherer, Janna [[e-mail]]
Sent: Wednesday, September 29, 2010 3:46 PM
To: Lopez, Dale; Brobst, Dennis; Henson, Belinda; Corporon, Joe
Cc: Sikes, Brant
Subject: Cc of letter mailed to Aquatic Toxicoloy Unit
Attachments: Elimination of Copper and Zinc as Causative Effluent Toxicants.tif; Moore County TOXICITY
TRE.xls
Please find attached the final report from the Moore County WPCP regarding the elimination of copper and zinc as
causative effluent toxicants. If you would like a hard copy mailed to you, please let me know.
Thank you,
Janna Scherer
Janna L. Scherer
Pretreatment Coordinator/Lab Supervisor
Moore County WPCP
[phone]
1

TubCic Works
Water PoCatfon ControCTtant
io94 .�ldcfor Road
Aberdeen, NC 28315
County of Moore
September 29, 2010
North Carolina Division of Water Quality
Environmental Sciences Section
Aquatic Toxicology Unit
Attn: Cindy Moore
1621 Mail Service Center
Raleigh, NC 27699-1621
RE: Moore County WPCP Permit # NC0037508
Elimination of Copper and Zinc as Causative Effluent Toxicants
Ms. Moore,
(gio) 281-3146--Tlione
(gio) 281-2o47- Fax
In Compliance with Option #3, as listed on the NOV dated November 21, 2009, Moore
County Water Pollution Control Plant is providing documentation that eliminates copper
and zinc as causative effluent toxicants. The final report is enclosed.
As stated in an e-mail sent on September 29, 2010, we apologize for the report being
slightly late. The report was due September 28, 2010 and is late due to an oversight on
our part. We apologize for any inconvenience this may have caused.
If you have any questions or require more information concerning this correspondence,
please contact me at your convenience.
Sincerely,
J. Brant Sikes, Superintendent
Moore County Water Pollution Control Plant
Cc: Belinda Henson, Fayetteville Regional Office
Dale Lopez, Fayetteville Regional Office
Joe Corporon, Point Source Branch
Marshall Hyatt- Water Management Division, USEPA Region IV, 61 Forsyth St.,
SW, Atlanta, OA 30303
Central Files
Dennis Brobst, Public Works Director, County of Moore

Elimination of Copper and Zinc as Causative Effluent Toxicants at the Moore
County Water Pollution Control Plant.
Date of Report: September 29, 2010
The Moore County Water Pollution Control Plant (MCWPCP) believes that copper and
zinc have been eliminated as causative effluent toxicants at the plant. As reported in a
letter to the Aquatic Toxicology Unit of the NC Division of Water Quality dated January
21, 2010, MCWPCP chose to conduct Toxicity Identification Evaluations (TIE's) to
provide results that definitively rule out copper and zinc as a cause of effluent toxicity.
Toxicity tests were conducted during December 2009, March 2010, and June 2010.
During each sampling event, a portion of sample was retained for a TIE on metals.
Should the toxicity test have resulted in a "fail", the metals would have been extracted
from the retained sample and another toxicity test would have been ran. The results of all
three toxicity tests are as follows
• December 2009 test: Pass (>100). The sample was split between three
different labs and all indicated a result of "Pass".
o March 2010 test: Pass.
o June 2010 test: Pass.
During each toxicity test, LTM sampling was conducted. The table below shows the
monthly copper and zinc results for the past year:
Tox Result
Cu 1
Cu 2
Zn 1
Zn 2
mg/L
mg/L
mg/L
mg/L
Sep-09
Fail
c-0_0187
0.014
0.046
0.060
LTMP
Oct-09
Fail (<10.25)
0.014
0.011
0.047
0.052
Nov-09
Pass(50.2)
0,014
0.010
0.051
0:050
. Dec-09
Pass(>100)
0.012
0.011
0.079
0.052
LTMP
Jan-10
0.012
0.009
0.068
0.045
Feb-10
0.009
0.009
0.044
0.033
Mar-10
Pass
• 0.007
0.014
. 0.039
0.060
LTMP
Apr-10
0.007
0.009
0.038
-0.036
May-10
0.008
.0.009
0.048
0.055
Jun-10
Pass
0.009
0.015
0.055
0.077
LTMP
Jul-10
0.013
0.016
0.056
0.062
Aug-10
0.011
0.012
0.063
0.056
As shown in the table, the concentrations of copper and zinc were not significantly higher
during the failed toxicity events. A review of the MCWPCP Long Term Monitoring
(LTM) data from 2004 - present revealed that copper consistently trends higher than the
Action Level of 7 ug/L, with no toxicity issues. LTM results, for copper, from 2004 -
present range from 7 ug/L to 18 ug/L. Several NC cases are documented in which

instream total copper ranged up to 378 ug/L with no chronic toxicity to Daphnia. Based
on all of the information described above, the MCWPCP believes that copper and zinc
have been eliminated as causative effluent toxicants at the plant. The results of the
toxicity tests conducted in December 2009, March 2010, and June 2010 are included as
attachments to this. report.
In addition to eliminating copper and zinc as causative effluent toxicants,,the MCWPCP
conducted a comprehensive Toxicity. Reduction Evaluation (TRE) in an attempt to
determine and eliminate the source of toxicity. The TRE. consisted of all of the
following:
o Review of LTM results from 2004 — present.
Investigation into SRI's as potential source of toxicity.
• Investigation into in-house chemical feed systems.
® Consideration of any equipment/process changes that could have contributed
to toxicity.
® Review of a similar toxicity failure event that occurred during 2006.
• Consideration of Commercial Lab. Subsequent samples were split between
multiple labs.
Investigation into TDS as source of toxicity.
o . Draining and cleaning of the chlorine contact basin.
• Repair of the flash mixer in the chlorine contact basin.
• Investigation into surfactants as a source of toxicity. (MBAS)
• A Priority Pollutant Analysis (PPA) was conducted simultaneously with the
November toxicity test. All parameters tested normal.
• Investigation into a chemical used by five of our permitted septic haulers.
® Review of all septic hauler data.
e Tested the hardness of the effluent in conjunction with a toxicity test.
A spreadsheet depicting the items included in the TRE and their results is included with
this report, as an attachment.

X
Sig a of Opera or in Responsible -Charge
�J^ 11Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 L e: 06/24/10
Facility: MOORE COUNTY NPDES#: NC0037508 Pipe#: 001 Count : M66 ;2°1.,.i ''''-
Laboratory Performinest: MERITECH, INC.. BY: -.--
Comments: Dilution Water Batch
#813 & 814 Used
-* PASSED: 17.32% Reduction *
Signature of Laboratory Supervisor
Work Order:
MAIL ORIGINAL TO:
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
-B 4 &n e t-a1- Bien arch --
Div. of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, N.0 27699-1621
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced
26
25
28
21
24
27
26
25
26
25
26
27
Adult (L)ive (D)ead 1L
L
Effluent %: 41%
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced
23
21
22
16
21
16
20
23
27
20
18
26
Adult (L)ive (D)ead
'Chronic Test Results
Calculated t = 3.939
Tabular t = 2.508
% Reduction = 17.32
145
% Mortality
Avg.Reprod.
0.00
Control
25.50
Control
0.00
Treatment
2
21.08
Treatment
2
Control CV
6.995%
% control orgs
producing 3rd
brood
100%
PASS FAIL
Check One
pH
Control
Treatment 2
D.O.
Control
Treatment 2
1st sample 1st sample 2nd sample
8.08
8.02
7.93
8.03
8.10
8.04
7.84
B.00
s s
t e t
a n a
r d r
t t
1st sample 1st
7.40
7.38
7.45
7.40
e
n
d
8.10
8.00
7.94
7.96
a
r
t
sample 2nd sample
7.54
7.30
7.64
7.26
e
n
d
7.55
7.27
7.56
7.21
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
Complete This For Either Test
Test Start Date: 06/16/10
Collection (Start) Date
Sample 1: 06/14/10 Sample 2: 06/17/10
Sample Type/Duration 2nd
1st . P/F
Grab Comp. Duration D
I S S
Sample 1 X 24.1 hrs L A A
U M M
Sample 2 X 23.4 hrs T P P
Hardness (mg/1)
Spec. Cond. (jimhos)
Chlorine (mg/1)
Sample temp_ at receipt(°C)
44
172
442
448
<0.1
<0.1
'0.7
2.1
%
%
%
%
%
a
%
%
%
%
%
%
%
%
9.5
%
%
%
%
Concentration
Mortality
start/end
LC50 =
95% Confidence Limits
Method of Determination
Moving Average _ Probit _
Spearman Karber _ Other
Note: Please
Complete This
Section Also
start/end
Control
High
r,,,,,.
pH
Organism Tested: Ceriodaphnia dubia
Duration (hrs) :
Copied from DEM form AT-1 (3/87) rev. 11/95 (DUBIA ver. 4.32)
D.O.

:lienfi 4N\0c;�
• Mini Chronic Pass/Fail Test: Ceriodaphnia dubia
Pipe*: 001 County: Date Start: 4l10
1PDES #: NC ct , 3--) 5v K Date / Time of Culture Transfer: (p .1./7 ' f5": %rAP" Time Start: / 0: 4-J")
)ilution Water: Lake Reidsville Date/ Time Neohates bom: j -L -t ) ~
"est Organism Source: Tray # Age of Neonates at Test Start: A. hours
Randomized:/ N Culture Tray Tenip: °C
roduction
2 3
stirred / Aerated for D.O.: Y
Control Organism Repj
Day #2 1
# Young Produced
Adults Live ./ Dead
Dav #5
# Young Produced
Adults Live / Dead
D7
#'Young Produced
Adults Live / Dead
0
0
Ir
0
5 6 7 8 9 10 11 12
l.-
0
0
Lr.
L-.
0
fL.--
1 2 3
L
15/d1
2
11
/ID
3
4
.3/7
L
11
L
5
L
5
la-
6
'L
;,
7 8 9 10 1 12
la
1
rf
8
'MD
L
bnc
L
3
11
t_
10 11 12
/3
it
I1
L
Total Produced •
gi
,),
fp?
if
;q
„• -
r2
-
Test Sample Organism Ikeproduction
Percent of Control producing third brood: / t %
2 3 4 5 6 7 8' 9 10 11 12
Effluent %: 41
Day #2 1
#Young Produced
Ci
0
0
0
0
0
0
0
0
0
0
0
Adults Live / Dead
L-
L-
L-
1..-
U
1--
L
1.- L-
I--
L.-
L
Dav #5
1
2
4
5
6
7
9 / 10 11 12
# Young Produced
' a
. 7'4'3
i
3 '
•-3 r
�9
,
it✓
t
(0,
- /j'
Adults Live / Dead
Li
L
'L,.(--
L
L
.
L
L
L
L_
L.
Day #7
1f 2
3
4
5
6
7
8
10 11 12
#Young Produced
, I
8'
J P
Jo
9
g-
%,
l'
)0
t'i
,(,),
Adults Live / Dead
t--t
L
L-
L.,
L
L
L
L
L
L
I--
L
Total Produced
Comments: is t)
A- Leek heal ,/
(7
•f
Ig
1st Renewal Date: Oh rs%rr2
2nd Reriewal Date: t /"2-i lr0
Arialyst(s): Yid kept,
Collection (Start)Dates:
Sample 1: �//`di 0
Incubator #: 1
Date End: 6( f t O
Time End: A:Ale) AM
Time: 9 AJvi
Time: g-; 3/,
Reviewed by:
• Sample 2: ()17 JJ 0
Sample Information
•
100% pH
G / C?
Duration
Sample 1
' -7,7.3
�•'-
2- ,r
hours
Sample 2
7, 6 2.
G
23. L)
hours
Batch #
Sample
Semple
Transfer Day`
0
2
5
t
,sl�;�+
Hardness
(mglL)
u
V�
�
2
'Y •y
�
's 0.j:i
u"��'
Za.,
�,�w �;r
ill F
.1'' � r1i•C••
Spec. Cond.
(umhos/cm)
,
�]
l
Q
�U
?_
�� v11°
Chlorine
(m9IL)
'' k
aft f ,tN
r��=
1 a4; .:
4O 1
40. i
Receipt Sample
Temp. CC/
i+;
F,s-
F1
bi• l
-2
1st Sample
Control
3,05
g, c2
Sample
7,93
S'.63
D.O.
India l final
1st Sample
Control
7.4d
3'K
Sample
?'1
�,
Temp.
nit al final
1st Sample
Control
Z51)
2,57%
Sample
Zti .&
267 /
initial final
Transferred by: Fed by:
Day 0 PO— fit 1--
Day 1 0/2--
Day 2
Day3
Day 4
Day 5 1/ir
Day 6 •
Terminated by: AV. -
1st Sample
5k,1t
og
,t'D
initial final
1st Sample
7,2to
initial final
1st Sample
*0,-7
79
7,5.`f
initial final
2nd Sample
5,10
1,q(a
Initial final
2nd Sample
tilo
7,1)
initial final
2nd Sample
zyG
final

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 03/25/10
Facility: MOORE COUNTY
X
: MERITECH, INC.
Signature of Laboratory Supefvi
NPDES#: NC0037508 Pipe#: 001 County: MOORE
Work Order:
North Carolina Ceriodaphnia
Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS 1 2 3
Comments: Dilution Water Batch
#795 & 796 Used
* PASSED: -4.58% Reduction
Environmental Sciences Branch
Div. of Water Quality
MAIL ORIGINAL TO: 3 NC DENR
1621 Mail Service Center .
• Raleigh, N.C. 27699-1621
Chronic Test Results
Calculated t = -1.130
Tabular t = 2.508
8 9 10 11 12 % Reduction = -4.58
#'Young Produced
16
22
23
23
22
21
22
21
23
23
23
23
Adult (L)ive (D)ead
L
Effluent %: 41%
TREATMENT 2 ORGANISMS 1 2 3 4
6
9 10 11 12
# Young Produced
27
23
22
19
20
25
23
21
25
24
24
21
Adult (L) ive (D) ead
145
% Mortality
Avg.Reprod.
0.00
Control
21.83
Control
0.00
Treatment 2
22.83
Treatment
2
Control CV
9.126%
% control orgs
producing 3rd
brood
91.7%
PASS FAIL
X
Check One
pH
Control
Treatment 2
D.O.
Control
Treatment 2
1st sample 1st sample 2nd sample
7.97
7.80
7:32
7.72
7.87
7.85
7.51
7.88
7.96
7.88
7.68
7.80
s s s
t e t e t
a 'n a n a
✓ d .r. d r
t t t
1st sample -1st sample 2nd
7.73
7.. 88
8.12
7.96
7.80
7.62
8.12
7,52
e
n
.d
sample
7.53
7.38
7.72
7.23
LC50/Acute Toxicity Test
(Mortality expressed as.% .combining replicates
Complete This For Either Test
Test Start Date: 03/17/10
Collection (Start) Date
Sample 1: 03/15/10 Sample 2: 03/18/10
Sample Type/Duration
Sample 1
Sample 2
Grab
Comp.
Duration
24.2 hrs
23'.9 hrs
2nd
1st P/F
D
I S S
L A A
U M M
T P P
Hardness(mg/1)
Spec. Cond.(µmhos)
Chlorine(mg/1)
Sample temp. at receipt(°C)
42
170
398
429
<0.1
<0.1
0.5
0.2
%
%
%
%
%
%
%
%
• %
%
%
%
%
%
%
%
%
%
%
%
Concentration
Mortality
start/end
LC50 = •%
95% Confidence Limits
%.-- -
Method of Determination
Moving Average _ Probit
Spearman Karber Other
Note: Please
Complete This
Section Also
start/end
Control
High
r.-r
•
pH
Organism Tested: Ceriodaphnia dubia
Duration(hrs) :
Copied from DEM form AT-1 (3/87) rev. 11/95 (DUBIA ver. 4.32)
D.O.

•11C1 IL I
IPDES #: NCO ;77,6-0 t=5 Date / Time of Culture Transfer: ' )I' ) O, Jt=i0 Ain Time Start:
►ilution Water: Lake Reidsville_
'est Organism Source: Tray #
stirred / Aerated for D.O.: Y
:0 7p.
Date / Time Neonates born: 3 • 1770 j'''S 4pi ): pin 1st Renewal Date: /,,ili U
Age of eonates at Test Start: qo hours 2nd Renewal Date: ' 1 zz/ic,
Randomized :tY / N Culture Tray Temp:;),q, °C Analyst(s): ML, Yi1'L
Collection (Start) Dates:
Control Organism Reproduction'
1 2 3 4
7 8 9 10 1.1
12
# Young Produced
C3:
Cp
0
O
0
0
.0
0
0
0
0
0
Adults Live / Dead
l.-
L- •
t--
L
t-
1--
lam-
L
'11...-
L-
L_
L
Day #5
# Young Produced
Adults Live / Dead
Day #7
# Young Produced
Adults Live / Dead
r
1 2 3 4
(; $ r
L, L.L qs,.- 04,
L L�
/a
5 6 7 8 9 10 11
• L. L_
•6 7 8• 9 1 h V/19
/
L
L_
L
12
L
Total Produced
1/6
aT1go2
z
Percent of Control producing third brood: /_ %
Test Sample Organism Reproduction
Effluent
• Day #2
# Young Produced
Adults live / Dead
Day #5
# Young Produced
Adults Live / Dead
Day #7
# Young Produced
Adults Live / Dead
/b6
1 2 3 4 5 6 7 8 9 10 11 12
1
L
b
5--
L-
0
L-
0
L
0
0
L_
0
3 4 5 6 7 8
5--
L-
L-.
Lf
3 Li
L �Lr
1 2 3 .4 5 6
/021
4/0
L.-
7
L
L.
L
ro�q
L_
1/
L_
L
0
L
0
L
L
0
10 11 12 •
L
L
5--
19 11, 1;
/l 'df
L_
it
Total Produced
I2.71Q2 2.3\1M I1 S5)-9-1a I f 1
Comments: 3 lt'e- c2 )'c 5 SPlie
Sample 1: "jib
Time End: 3' 70pm
Time: S2•'3;,4/\',
Time:
Reviewed by:
Sample Information
Sample2: �Ie�lly
100% pH
G/C?
Duration
Sample 1
Sample 2
(a. gq
223
C•
2 7. hours
Z 1 c• hours
Batch #
76.1''
1 1(4
Sample
Sa2ple
,..__
Transfer Day
0
2
5
g
'
.S +
{
Hardness
(msfL)
y 2
At,
--->
Spec. Cond.
(umhoslcm)
170
,V'^�
V
398
L+ L
-1
Chlorine
(mg!L)
jii '
Ir
.1'''
8:. ytz'' k
i rtil�ll1 .40. 1 l
.40ii
Receipt Sample
Temp. CC)'
�i } \J
%
0 Z.-
PR
lst Sample
Control
787
.30
Sample
D.O.
7r7 Z
initial, - final
1st Sample.
Control
773
Sample
Tema.
8•ii
initial final
1st Sample
Control
24'.2-2915
Sample
214• 51-9t
lnitlal
Tina
Transferred by. . Fed by
Day l7> ` ML.
Day 1
Day 2 1(L- 6;/t
Day 3 tiL�
Day 4
Day 5
Day 6 't%p
Terminated by:
1st Sample
7,87
761
initial final
1si Sample
.iyZ
�L-
7L2
Initial final
1st Sample
111,5
ZI{,v
2nd Sample
67,
Z 80
initial final
2nd Sample
7 r1
•7,72 7,23
initial flnal
2nd Sample
Zti?
-10
«•Y
nitlal final initial final

CAS rer,�-ea oj' = P leE MIA1'�'• 02.5 norms
Effluent Aquatic Toxicity Report Form/Phase II Chronic Ceriodaphnia
Laboratory Performing Tes
nature of O.R.C,
Facility -.Moon- CO L frk NPOES#: NC no V150R Pipetf�County
Comments );Itikicift Wo4tr f oal, 3q'T9 a
* - BD ascot. ,S;n jlc in vets ion in 11-ie' 62%
•11-4t _Con[enfra-fiarl.
r; `-e,Lh Tyke..
Signature of Lab Supervisor
Semple Information
Sam • le 1
uT J — Control
Collection Start Date
Grab
Composite
(Duration)
Hardness(mgll
Spec.Cond.(pmhos/
Chlorine(mg/i
-Pi- 0
.011911111
MINIIILTAIII
.CO
112 4o
'10 iqo
Sample temp. at receip i
!, 5 'C
0. S
Control
Effluent%
!a. 25
Effluent%
Effluent%
41
# Young
Adult
(L)ive (D)ead
# Young
Adult
(L)ive (D)ead
# Young
Adult
(L)ive (D)ead
# Young
Adult
(L)ive (D)ead
1 • 2
24
L
1
_L.
1
29
L
1
25
'-1
L
2
4
L
3
21
L
3
L
2<2
L
z,1
L
4
23
L
19
L
2 3 .4
5
29
5
21
L
Qraanism#
6 7 8
z$
L
6
L._
7
20
L
27
Test
Information'
Start Date End Data
l2,il4/ / /C(1
Start Time
4!
F.nd TIme
3:00ti
Start
Renew1
Renew2
Start
Renew1
Renew2
Treatment
pH Initial
pH Final
0.0. Initial
D.O. Final
Temp. Initial
Temp. Final
9
30
L
1
0 11
1G
L
10o%
121
8.41
1000�
1.2.8
r1.Ito
B. 48
106
q.'io
8.23
Control
'1.9I
'L SJ
1.slo
Control
'1.88
05
7.6C
Control
13.00
11$o
65
1.51
24.1
1.ia9
252
?4b
2'1, 3
1.10
26:2
�T.61
ZS. I
763
24.5
25.2
✓_, 9 10 11 12
2$
L.
22.
L
25
L
5 6 7 8 9 10
19.
L
5
29
L
6
22
L
23
2.7
L
7 8 9 10
Wan
M9Pn
2 4 24 20 2015 15 ! 9 22 '1 21.6
Effluent%
# Young
18
241
18
25
9
1°i
b
Zia
2,g1
I fl
[[•b
$2, f(L)iveeac
L
L
L_ 1._
L.
L
L L
L
Effluent%
i goo i
# Young
2,
Adult
(L)ive (D)ead
4
z 2
LeL
6 7 8 9 10
2.1
2
L
L
11
L
2
L
3 .7
L
Red
2'f 8
Mean
2A.4
25.2
24,6
24.'4
Chronic Test Raab Its
Final Control Mortality 9-)
% Control 3rd Brood
Control Repro CV
Conttrolour mortality
0 ofj_o_
Significant?
0
90
15•5
0 of 10
©
Final Mortality Slgnifi
of
•
o Cone
•
,
Reproduction Analysis:
Repro. LOEC=y l00%• NOEC=_100 %
Method•al.A.Q.n&V,s / a tt
Normal Di9trib? Method:6Amarialti
Statistic: 0, 6'i o Critioal:_l_L
Equal Variances? LIQ.S Method:,�ai,Aid
Statistic: 5'l Crltical:
Non•Perametric Analysis (if apoilcablej:
Method:
Effluent % Rank Sum CritiAal Sum
Overall Analysle:
Result = PASS/FAIL or
Test LOEC= ?IOC/ %: NOEC=jj D__°%
Chronic Valuer- 2-_10o %
MAIL
TO:
ATT: Environmental Sciences Branch
Div, of Water Quality
N_C. DENR
1621 Mail Service Center
Raleigh. N.C. 27699-1621
'Should use highest teat concentration or
highest concentration with 0.0. >5.0 mgl
1% RNducllon from Conn! Rermductbn Moon
DWQ form AT-3 (8/91) Rev. 11/95
RECEIVED 0.3-01-'10 10:12 FROM- 3363421522
TO- Moore UIPCT P0002/0002

Efflueti:. at
eport\Ehase II Chronic Ceripodaphnia jU'tiO9
1 \v/ •
C-�
Facility Moore County WWTP NPDES#NC 0037508
Laboratory P thing Tejj Research
X
if�fdture of O.RC.
Inc.
tur of Lab S '. ervisor
Pipe# 001
olinty?
Comments: Final Effluent [ -
663238/663476
Satnplelnformatioii
Sample 1
Sample 2
Control
Collection Start Date
12/14/09
12/17/09
:1 {i7, F ' i� y+:
Grab
- hi x'sb_a .
Composites}`y
y'
(Duration)
Hardness (mg/I)
24 hrs
24 hrs.
r+ a;= i,
:.....,.,... ,.
e'r=:;fit
4''#' K%r..-,,,, t
48
Spec.Cond. (timhos/cm)
377
394
182
Chlorine (mg/L)
0.03
0.03
_-t.j, - t . '
Sample temp. at receipt
2.1°C
3.0°C
, `= x • .,
Control
Effluent %
10.25
Effluent %
20.5
Effluent %
41
Effluent%
82
Effluent %
100
1
Organism#
3 4 5 6 7
8
Test
Information*
Start Date
12/16/09
End Date
12./23/09
Start Time
1138 AM
End Time
11:40 AM
Treatment
PH Initial
pH Final
D.O. Initial
D.O. Fmal
Temp. Initial
Temp. Final
Start
Renew 1
Renew 2
Start
Renew 1
_ Renew 2
% 100
�
100.0%
100.0%
Control
Control
Control
7.30
7.28
7.24
6.93
7.10
6.94
7.55
7.36
7.32
7.09
7.15
6.93
8.3
8.5
8.6
8.6
8.5
8.6
8.3
8.4
8.3
8.2
8.0
8.L
24.3
24.6
24.2
24.4
24.6
24.2
24.4
24.6
24.8
24.4
24.6
24.8
9
10
11
12
Mean
#Young
24
22
21
26
22
24
25
24
26
24
23.9
Adult
(L)ive (D)ead
LLLLL
L
L
L
L
L
ty.
air r'
1
2
3
4
5
6
7
8
9
10
11
12
Mean
# Young
24
28
24
27
24
22
25
23
26
23
24.6
Adult
(L)ive (D)ead
L
L
L
' L
L
L
L
L
L
L
% Red
-2.93
1
2 3 4
5 6 7 .8
9 10 Mean
# Young '
23
19
24
23
27
24
24
24
22
23
23.3
Adult
% Red
(L)ive (D)ead
L
L
L
L
L
L
L
L
L
L
2.51
1
2
3
4
5
6
8
9
10
Mean
# Young
28
22
24
1
26
24
25
24
25
20
23
24.1
Adult '
-
% Red
(L)ive(D)ead
LLL
L
L
L
L
L
L
L
-0.84
1
2
3
4
5
6
7
8
9
10
Mean
# Young
20
21
18
26
25
24
' 27
21
23
18
22.6
Adult
(L)ive (D)ead
L
L
L
L
L
L
L
L
L
L
% Red
5.44 '
2
4
6
7
8
9
10
Mean
# Young
22
26
22
22
23
24
20
22
23
23
22.7
Adult
(L)ive (D)ead
LLL
L
L
L
L
L
L
L
% Red
5.02
Chronic Test Results
Final Control Mortality'/
% Control 3rd Brood
Control Repro CV
0
100
7.086
48 Hour Mortality
Control IWC
0 of 10 0 of 10
iignifican
Y
Fiinal Mortality Significant
or
No Conc.
Reproduction Analysis:
Repro. LOEC = >100% NOEC = 100 %
Method: Dunnett's T-test
Normal Distrib? yes Method: Kolmogorov
Statistic: - 0.658 Critical: 1.04
Equal Variances? yes Method: Bartlett's
Statistic: 6.23 Critical: 15.1
Non -Parametric Analysis (if applicable):
Method:
Effluent% Rank Sum Critical Sum
Overall Analysis:
Result = PASSIFAIL or
TestLOEC= >100 %: NOEC= 100 %
Chronic Value= >100
MAIL
TO:
ATT: Environmental Sciences Branch
Div. Of Water Quality
N. C. DEAR
1621 Mail Service Center
Raleigh, NC 27699-1621
* Should use highest test concentration or
highest concentration with D.O. >5.0 ing/1
1% Reduction from Control Reproduction Mean
Copy DWQ form AT-3 (8/91) Rev. 11/95