NC0039586_Effluent Aquatic Toxicity Reports_20210611

DUKE Plant
R. Dills
laito ant Manager
Harris Nuclear Plant
ENERGY 5413 Shearon Harris Rd
New Hill, NC 27562-9300
Serial: RA-21-0172
Certified Mail Number: 7014 2120 0003 3196 6562 R ECEIVE�
Return Receipt Requested
North Carolina Division of Water Resources �N 2021
Water Sciences Section /Aquatic Toxicology Branch
1623 Mail Service Center NCDEQ/pWR/NPDES
Raleigh, North Carolina 27699-1623
Subject: Duke Energy Progress, LLC
Harris Nuclear Plant and Harris Energy and Environmental Center
National Pollutant Discharge Elimination System (NPDES)
Permit Number NC0039586, Effluent Aquatic Toxicity Reports
Ladies and Gentlemen:
In accordance with Part I, Section A (8) of NPDES Permit Number NC0039586, enclosed are
the May 2021 Effluent Toxicity Reports for the Harris Nuclear Plant and Harris Energy and
Environmental Center.
Please contact Mr. Bob Wilson at [phone] or Mr. Don Safrit at [phone] if there
are any questions regarding this information.
I certify, under penalty of law, that this document and all attachments were prepared under my
direction or supervision in accordance with a system designed to assure that qualified personnel
properly gather and evaluate the information submitted. Based on my inquiry of the person or
persons who manage the system, or those persons directly responsible for gathering the
information, the information submitted is, to the best of my knowledge and belief, true, accurate,
and complete. I am aware that there are significant penalties for submitting false information,
including the possibility of fines and imprisonment for knowing violations.
Sincerely,
John . Dills
Enclosure: May 2021 Effluent Toxicity Reports

North Carolina Division of Water Resources
Water Sciences Section /Aquatic Toxicology Branch
Serial: RA-21-00172 / page 2
bc: Della Allen
Brice Peters
Don Safrit
Bob Wilson
Darlene Wallace
Nuclear Records

North Carolina Division of Water Resources
Water Sciences Section /Aquatic Toxicology Branch
Serial: RA-21-0172
Enclosure
Harris Nuclear Plant and Harris Energy and Environmental Center
NPDES Permit Number NC0039586
May 2021 Effluent Toxicity Reports
(5 pages including cover)

PO Box 7565
Asheville, NC 28802
Phone: [phone]
Fax: [phone]
Effluent Toxicity Report Form -Acute Pass/Fail Date: May 17,2021
Facility: Duke Energy Corporation NPDES#: NC 0039586 Pipe it 006 County: Wake
Shearon Harris Plant, Outfall 006
Laboratory Performing Test: Environmental Testing Solutions, Inc.,Certificate#037 Comments
Signature of Operator in Responsible Charge(ORC): ,.qlpry f. Q oln< , .
ORC Phone/E-mail: ci 1114, as q- a 41n(e r1.//a.. 4/lefs P_du.kc- 9 y,caM Project#: 15969
Signature of Laboratory Supervisor: ,/„y/ Sample#: 210511.07
e-Mail to: ATForms.ATBPncdenr.gov
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh,NC 27699-1621
North Carolina Acute Pass/Fail Toxicity Test
Collection Date: 05-10-21 Organism Tested
Collection Time: 0625
Test Start Date: 05-12-21 Pimephales promelas
Sample/Type/Duration
Grab Comp. Duration c Control 7.93 7.85
c pH(S.U.)
co. X 24-h 0
— Treatment 7.61 7.56
— o
H `
Alkalinity(mg CaCO3/L) 59 9 w
in
Hardness(mg CaCO3/L) 91
Conductivity(µmhos/cm) 310 108 Control 7.8 7.8
D.O. (mg/L)
Total Residual Chlorine(mg/L) <0.10 Treatment 7.9 8.0
Sample Temp.at Receipt 1°C> 1.0
Mortality Replicate Mean Mortality
Treatment 1(Control) A B C D
0% 0% 0% 0% 0.0%
Treatment 2(Exposure) A B C D
Concentration
Tested 90% 0% 0% 0% 0% 0.0%
Note: If mean control mortality exceeds 10%,the test is considered invalid.
Calculate using Arc-Sine Square t-Stat/ Rank Sum NC PASS X
Root transformed data. 1-Tailed Critical NC FAIL .
I
If the absolute value of the calculated t is less than or equal to the absolute value of the tabular t,check PASS.
If the absolute value of the calculated t is greater than the absolute value of the tabular t,check FAIL.
If all vessels within each treatment have the same response but the treatment two response is greater than the control,check fail.
DWR Report Form AT-2

.� 351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Duke Energy Progress NPDES#: NC0039586 Pipe#: 006 County: Wake
Shearon Harris Plant Purchase order 21867668
Species: Pimephales promelas Effluent dilution: 90%
Test type: 24-hour Pass/Fail Acute Parameter code: TGE6C
Sample information: (to be completed by sample collector)
Composite sample: Sample location: Q ta.I-f a.11 0040 HAI IP
Start date: 5-16-eL l Time: Ono 01,5 Volume collected for testing: I Q,t�
End date: 5-- it-21 Time: 0 6a 3— Number of containers filled for testing: 1 J
Number of samples per hour: l Method of transport to laboratory: ('.
Chilled during collection? '1 t 5 Comments: C n 12_,r- i. e Y`
If chilled,specify temperature: J. 5
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample collected by:
a?e✓►*t4I / tfii4 iJ06b0
• lr,„, signat� a�ttima
Relinquished by: Received by: SAXc 'rtPh1 as-*AA
Print Signature Data and time Print Signature Data and limn
Relinquished by: Received by:
. Pam si�tw.: nano aria�m� Prim sip ��. .�Duty ancrsce
Sample receipt information:
(to be completed by ETS personnel)
Relinquished to ETS by: Stl t P T QAI'ok 1f i a 1\ Aco Received at ETS by:
a SS�lti OS—►(•tl
"W ‘4150
Pnnt Signature Date and urea Pam Signature Date and time
Custody seals intact?: E -LJ ED Sample temperature upon receipt at ETS(°C): O .
Yee No Na used
Samples received in good condition?:
Yc Na Total residual chlorine upon receipt at ETS: EJ Ea/
(DPD Presence/Absense Indicator,MDL=0.10 mg/L) P"""t Abecnt
Tracking number: /i �t
Project number: (S` A. Sample number: 2(OS 11. (J '
Comments:

Y.-. y PO Box 7565
Asheville, NC 28802
Phone: [phone]
Environmental Testing Solutions.inc. Fax: [phone]
Effluent Toxicity Report Form -Acute Pass/Fail Date: May 17, 2021
Facility: Duke Energy Corporation NPDES#: NC 0039586 Pipe#: 007 County: Wake
Shearon Harris E&E Center, Outfall 007
Laboratory Performing Test: Environmental Testing Solutions, Inc.,Certificate#037 Comments
Signature of Operator in Responsible Charge(ORC): I? atbiy<_.—)
ORC Phone/E-mail: q gY-�a 9-a 9/e/p C1e iIQ. cl,l/Gn duk cnergv 401) Project#: 15968
Signature of Laboratory Supervisor: r�},4/ Sample#: 210511.06
e-Mail to: [e-mail]
Or Mail Original to: North Carolina Division of Water Resources
Water Sciences Section/Aquatic Toxicology Branch
1621 Mail Service Center
Raleigh,NC 27699-1621
North Carolina Acute Pass/Fail Toxicity Test
Collection Date: 05-10-21 Organism Tested
Collection Time: 0705
Test Start Date: 05-12-21 Pimephales promelas
Sample/Type/Duration
Grab Comp. Duration Control 7.93 7.85
c pH(S.U.)
X 24-h x Treatment 7.70 7.68
x
0
0
E-
a
Alkalinity(mg CaCO3/L) 59 C
v1 w
Hardness(mg CaCO3/L) 91
Conductivity(µmhos/cm) 310 2860 Control 7.8 7.8
D.O.(mg/L)
Total Residual Chlorine(mg/L) <0.10 Treatment 7.7 7.8
Sample Temp.at Receipt(°C) 1.0
Mortality Replicate Mean Mortality
Treatment 1(Control) A B C D
0% 0% 0% 0% 0.0%
Treatment 2(Exposure) A B C D
Concentration
90% 0% 0% 0% 0% 0.0%
Tested
Note: If mean control mortality exceeds 10%,the test is considered invalid.
Calculate using Arc-Sine Square t-Stat/ Rank Sum NC PASS X
Root transformed data. 1-Tailed Critical NC FAIL
If the absolute value of the calculated t is less than or equal to the absolute value of the tabular t,check PASS.
If the absolute value of the calculated t is greater than the absolute value of the tabular t,check FAIL.
If all vessels within each treatment have the same response but the treatment two response is greater than the control,check fail.
DWR Report Form AT-2

!. x •.rs
351 Depot Street
Asheville,NC 28801
Phone: [phone]
Fax: [phone]
_ Environmental Testing Solutions,Inc.
Whole Effluent Toxicity Chain-of-Custody Form
Facility: Duke Energy Progress NPDES#: NC0039586 Pipe#: 007 County. Wake
Shearon Harris E& E Center Purchase order: 2187668
Species: Pimephales promelas Effluent dilution: 90%
Test type: 24-hour Pass/Fail Acute Parameter code: TGE6C
Sample information: (to be completed by sample collector)
Composite sample: Sample location: e)/2---'a /f n ) ( HE-Ey
Start date: 5--I - l Time: O 7O$ Volume collected for testing: / 3 e .1
End date: ,�- j(-a 1 Time: Q '70,ej Number of containers filled for testing: I
Number of samples per hour: _/ Method of transport to laboratory: (AGr /'le r
Chilled during collection? y>r 5 Comments:
If chilled,specify temperature: /. O
Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample must be<6.0°C upon receipt at the laboratory.
1
1 Sample custody: (to be completed by sample collector and facility personnel)
BY SIGNING BELOW,I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE
Sample collected by:
D /la-R Allen 0 (ia._ -l/- 1
Pour Signature Data and tune
Relinquished by: Received by: t
Pont Signature Dam awl tune Pont Signature Dam and Limo
Relinquished by: Received by:
Prim Signature Dam and time Prim Signature Dale and time
Sample receipt information: (to be completed by ETS personnel)
Relinquished to ETS by: Received at ETS by:
�P TPJ�N1 oS-1E tit .i M
Sr\ N.51) 3-SJMtxa� °S• tLiStD
Prim Signature tune
Date and Prim m me Signature Da and ti
^
Custody seals intact?: t 1 Sample temperature upon receipt at ETS(°C):
Yee No Na usedI`O .C.
Samples received in good condition?:
Yes No Total residual chlorine upon receipt at ETS: I i �,
Nles (DPD Presence/Absense indicator.MDL=0.10 mg/L) Pre'a° AbsentTracking number:
Project number: ISitrk Sample number: 2.1 OS II.010
Comments: