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HomeMy WebLinkAboutNC0024406_Application_20210517CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Belews Creek, North Carolina NPDES Permit # NC0024406 Duke Energy Environmental Sciences Huntersville, NC April 2021 4's DUKE ENERGY., CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Contents Executive Summary 1 1 Introduction 2 1.1 Physical Description 2 1.2 Thermal Permitting History 6 1.3 Environmental Monitoring History 6 1.4 Station Operations and Weather Characteristics 7 2 Study Goals and Objectives 12 3 Methods 13 3.1 Temperature Analysis 15 3.2 Limnology 15 3.3 Planktonic Community 18 3.4 Habitat Formers 18 3.5 Benthic Macroinvertebrate Community 19 3.5.1 Mussel Community 19 3.6 Fish Community 22 3.6.1 Fish Community Assessment 22 3.6.2 RIS Population Assessment 22 3.6.3 Fish Community Similarities 23 3.6.4 Seasonal RIS Distributions 23 3.6.5 Reference Lake 23 3.7 Other Vertebrate Wildlife 25 3.8 Endangered Species 25 4 Results and Discussion 25 4.1 Temperature Analysis 25 4.1.1 In -Situ Thermal Monitoring 25 4.1.2 Satellite Imagery 31 4.2 Limnology 36 4.2.1 Water Quality 36 4.2.2 Water Chemistry 39 4.2.3 Chlorophyll -a 43 4.3 Plankton Rationale 43 4.4 Habitat Formers 44 4.5 Macroinvertebrate Rationale 49 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.5.1 Mussel Community 50 4.6 Fish Community 52 4.6.1 Fish Community Assessment 52 4.6.2 RIS Population Assessment 57 4.6.3 Fish Community Similarities 67 4.6.4 Seasonal RIS Distributions 69 4.6.4.1 Mayo Reservoir Comparison 71 4.7 Other Vertebrate Wildlife 78 4.8 Endangered Species 78 5 Balanced and Indigenous Assessment 79 6 References 80 Tables Table 1-1. Net capacity factors, expressed in percent (%), and monthly average discharge water temperatures (measured at CCW discharge) for BCSS during 2017 8 Table 1-2. Net capacity factors, expressed in percent (%), and monthly average discharge water temperatures (measured at CCW discharge) for BCSS during 2020 8 Table 1-3. Number of flow releases and daily average water temperatures at spillway compliance point for BCSS during 2017 and 2020. 9 Table 3-1. Analytical methods and reporting limits for parameters monitored in Belews Lake in 2020...17 Table 4-1. Summary of surface (0.3 m) water quality measurements made in Belews Lake during 2020 monitoring period. 38 Table 4-2. Summary of nutrients, major anions, and physical parameters in Belews Lake during 2020. Non -detect values are presented to the reporting limit. 41 Table 4-3. Summary of major cations, metals, and hardness (calculated) in Belews Lake during 2020. Non -detect values are presented to the reporting limit. 42 Table 4-4. Chlorophyll -a concentrations (in µg/L) collected semiannually from five zones in Belews Lake during 2020. 43 Table 4-5. Mean (and range) of water quality parameters for each zone in Belews Lake during spring 2020 electrofishing. 52 Table 4-6. Number of fish collected from electrofishing within six zones of Belews Lake during spring 2020. 52 Table 4-7. Percent pollution tolerance, trophic guild, and percent of hybrids for fish collected from electrofishing within six zones of Belews Lake during spring 2020. 54 Table 4-8. Mean (and range) of water quality parameters for each zone in Belews Lake during fall 2020 gill netting. 54 Table 4-9. Number of fish collected from gill netting within six zones of Belews Lake during fall 202055 ii CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 4-10. Mean (and range) of water temperature for each zone in Belews Lake during winter and summer 2020 electrofishing and gill netting. 69 Table 4-11. Numbers of RIS collected from electrofishing within six zones of Belews Lake during quarterly sampling in 2020. 70 Table 4-12. Numbers of RIS collected from gill netting within six zones of Belews Lake during quarterly sampling in 2020. 70 Table 4-13. Mean (and range) of water temperature for each zone in Mayo Reservoir during quarterly 2020 electrofishing and gill netting. 71 Table 4-14. Percent pollution tolerance, trophic guild, and percent of hybrids for fish collected from electrofishing within three zones of Mayo Reservoir during spring 2020 71 Table 4-15. Numbers of each species captured in Belews Lake and Mayo Reservoir during quarterly sampling with electrofishing and gill nets during 2020. Species richness and Shannon's diversity index (H) are also shown 77 Table 4-16. Location, behavior, and number of aquatic, vertebrate wildlife observed in the Belews discharge arm (2016-2020). 78 Figures Figure 1-1. Roanoke River drainage in North Carolina and Virginia, and location of Belews Lake. 4 Figure 1-2. BCSS location on Belews Lake, associated tributaries, and other site features. 5 Figure 1-3. Mean monthly air temperatures recorded near Belews Lake, North Carolina during current study period compared to 2010-2019 average 10 Figure 1-4. Total annual precipitation recorded at Belews Lake during current study period compared to 2010-2019 average (horizontal line). Historical data collected from USGS monitoring station at Pine Hall, NC 11 Figure 1-5. Total monthly precipitation recorded at Belews Lake during 2017 and 2020 compared to 2010 — 2019 monthly averages. Historical data collected from USGS monitoring station at Pine Hall, NC 11 Figure 3-1. Limnological (water quality and chemistry) sample locations in Belews Lake. 14 Figure 3-2. Aquatic plant species presence/absence sample locations in Belews Lake. 19 Figure 3-3. Fish and mussel sampling locations, and zones of thermal influence in Belews Lake. 21 Figure 3-4. Fish sampling locations and zones in Mayo Reservoir. 24 Figure 4-1. Surface water temperatures from temperature loggers at Belews Lake. Data cover the 2017 monitoring period. For the box plots, the line represents the median, mark "x" represents the mean, boxes depict 25th and 75th percentiles, and whiskers depict 10th and 90th percentiles 26 Figure 4-2. Isotherm plots for main lake (top) and discharge arm (bottom) areas from monthly water temperature profiles on Belews Lake, North Carolina. Data cover the 2017 monitoring period Temperatures are in degrees Celsius 27 i i i CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Figure 4-3. Isotherm plots for main lake (top) and discharge arm (bottom) areas from monthly water temperature profiles on Belews Lake, North Carolina. Data cover the 2017 monitoring period Temperatures are in degrees Celsius 28 Figure 4-4. Isotherm plots for main lake (top) and discharge arm (bottom) areas from monthly water temperature profiles on Belews Lake, North Carolina. Data cover the 2017 monitoring period Temperatures are in degrees Celsius 29 Figure 4-5. Isotherm plots for main lake (top) and discharge arm (bottom) areas from monthly water temperature profiles on Belews Lake, North Carolina. Data cover the 2017 monitoring period Temperatures are in degrees Celsius 30 Figure 4-6. Belews Lake, extreme summer thermal plume map (July 27, 2002). 32 Figure 4-7. Belews Lake, extreme winter thermal plume map (January 4, 2018). 33 Figure 4-8. Near extreme summer scenario isotherm plot on Belews Lake. Data gathered from historical Duke Energy water temperature profile data on August 27, 2002. Temperatures in degrees Celsius. 34 Figure 4-9. Near extreme winter scenario isotherm plot on Belews Lake. Data gathered using historical Duke Energy water temperature profile data on January 25, 2018. Temperatures in degrees Celsius. 35 Figure 4-10. Temperature (left panels) and dissolved oxygen (right panels) profile plots of Belews Lake during (top to bottom) February, April, July, and October 2020. Temperatures are in degrees Celsius, and dissolved oxygen is in mg/L. 37 Figure 4-11. Aquatic vegetation population densities and distribution noted at survey locations. 45 Figure 4-12. Distribution of Water Willow Justicia americana at sampled sites by zone. 46 Figure 4-13. Aquatic vegetation population densities and distribution at survey locations by thermal influence zone 47 Figure 4-14. Percentage of points surveyed found to be vegetated per zone of thermal influence by density class (1-4). 48 Figure 4-15. Species summary of vegetated points by zone of thermal influence 49 Figure 4-16. Mean catch rate (CPUE) by number of all freshwater mussels collected in 2020 within six zones in Belews Lake 51 Figure 4-17. Mean length of Eastern Elliptio collected in 2020 within six zones in Belews Lake. Error bars are 90% confidence intervals. 51 Figure 4-18. Mean catch rate (CPUE) by number (top panels) and by weight (bottom panels) of all species collected within six zones from electrofishing in Belews Lake during spring 2020. Error bars are 90% confidence intervals. Letters over bars indicate significance at a = 0.10. 53 Figure 4-19. Mean catch rate (CPUE) by number (top panels) and by weight (bottom panels) of all species collected within six zones from gill netting in Belews Lake during fall 2020. Error bars are 90% confidence intervals. 56 Figure 4-20. Mean catch rate (CPUE) by number of stock size and larger centrarchid RIS collected within six zones from electrofishing in Belews Lake during spring 2020. Error bars are 90% confidence intervals. Letters over bars indicate significance at a = 0.10 58 iv CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Figure 4-21. Length -frequency of Largemouth Bass (left panels) and Alabama Bass (right panels) collected within six zones from electrofishing in Belews Lake during spring 2020. 60 Figure 4-22. Length -frequency of Bluegill (left panels), Redbreast Sunfish (center panels), and Redear Sunfish (right panels) collected within six zones from electrofishing in Belews Lake during spring 2020. 61 Figure 4-23. Mean catch rate (CPUE) of Channel Catfish and Gizzard Shad collected within six zones from gill netting in Belews Lake during fall 2020. Error bars are 90% confidence intervals. Letters over bars indicate significance at a = 0.10 63 Figure 4-24. Length -frequency of Channel Catfish (left panels) and Gizzard Shad (right panels) collected within six zones from gill netting in Belews Lake during fall 2020. 64 Figure 4-25. Largemouth Bass (left panels) and Alabama Bass (right panels) condition (relative weight) by zone of Belews Lake and by length category for fish collected during fall 2020. The horizontal line represents the median for each zone, the boxes represent the 25th and 75th percentile, and the whiskers show 10th and 90th percentiles. 65 Figure 4-26. Bluegill (top left panels), Redbreast Sunfish (top right panels), and Redear Sunfish (bottom panels) condition (relative weight) by zone of Belews Lake and by length category for fish collected during fall 2020. The horizontal line represents the median for each zone, the boxes represent the 25th and 75th percentile, and the whiskers show 10th and 90th percentiles 66 Figure 4-27. Channel Catfish (left panels) and Gizzard Shad (right panels) condition (relative weight) by zone of Belews Lake and by length category for fish collected during fall 2020. The horizontal line represents the median for each zone, the boxes represent the 25th and 75th percentile, and the whiskers show 10th and 90th percentiles. 67 Figure 4-28. Bray -Curtis similarities of fish CPUE from spring electrofishing in Belews Lake during spring 2020. 68 Figure 4-29. Bray -Curtis similarities of fish CPUE from fall gill netting in Belews Lake during fall 202068 Figure 4-30. Mean catch rate (CPUE) by number of stock size and larger RIS collected from electrofishing in Mayo Reservoir during spring 2020. Error bars are 90% confidence intervals. 72 Figure 4-31. Length -frequency of centrarchid RIS collected from electrofishing in Mayo Reservoir during spring 2020. 73 Figure 4-32. Mean catch rate (CPUE) by number of stock size and larger RIS collected from gill netting in Mayo Reservoir during fall 2020. Error bars are 90% confidence intervals. 74 Figure 4-33. Length -frequency of RIS collected from gill netting in Mayo Reservoir during fall 2020 75 Figure 4-34. Condition (relative weight) of RIS in Mayo Reservoir for fish collected during fall 2020. The horizontal line represents the median for each species, the boxes represent the 25th and 75th percentile, and the whiskers show 10th and 90th percentiles 76 v CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Appendices Appendix A Final BCSS 316(a) Study Plan Appendix B Real Statistics® Statistical Analysis Appendix C Box and whisker plots depicting historical analytical data compared to 2020. Appendix D RDL and LOQ values for Nutrient Parameters collected in 2020 at Belews Lake Appendix E Tables of fish caught seasonally from Belews Lake during 2020 using electrofishing and gill nets. vi CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Executive Summary An initial 316(a) demonstration was conducted at Belews Lake to examine the physicochemical characteristics, in -lake productivity, habitat formers, mussel community, fish community, and other vertebrate wildlife during 2020, in accordance with the National Pollutant Discharge Elimination System (NPDES) permit (No. NC0024406) for Belews Creek Steam Station (BCSS) and the North Carolina Department of Environmental Quality (NCDEQ) approved study plan. This report presents operational and environmental data collected during the year and compares the information with historical data. The primary objective of this demonstration was to assess the impact of the thermal discharge from BCSS on the aquatic biological community populations in Belews Lake. Duke Energy has one permitted thermal discharge to the Dan River from the Belews Lake spillway. To evaluate the impacts of the thermal discharges on Belews Lake, six distinct zones were delineated. Zones were lettered from A to F in order of thermal impact with A being the most impacted. Zone A represented the area nearest the condenser cooling water (CCW) discharge, Zone B was adjacent to Zone A in the discharge arm of Belews Lake, and Zone C represented the area of the main lake influenced by the CCW discharge canal exit. Zones D and E represented areas with less thermal influence and F represented background conditions that were not subject to thermal influence. Belews Lake was characterized as oligotrophic based on long-term and current nutrient and chlorophyll -a concentrations. Chlorophyll -a concentrations during 2020 were comparable to historical data and were below the NC state water quality criteria (40 µg/L) at all monitoring locations. Seasonal water quality and chemistry data continued to affirm that Belews Lake provides a suitable physicochemical environment for sustaining a balanced and indigenous biological community. Similarly, data collected for other biological communities (e.g., freshwater mussels, habitat formers) also support that Belews Lake is suitable for sustaining a balanced indigenous community (BIC). A total of 10,767 fish representing 24 distinct species (plus one hybrid complex) within eight families were collected from Belews Lake during 2020. Bluegill were the most abundant species (58% of the fish captured), and annual catch rates of fish during spring 2020 were generally similar to those noted in historical biological reports (Duke Energy 2005). The fish community found in the thermally influenced zones of Belews Lake (Zones A, B, and C) encompassed multiple trophic guilds (e.g., insectivores, omnivores, and piscivores) supporting a balanced indigenous fish community. Additionally, fish captured in the thermally influenced zones had similar proportions of pollution tolerance to the associated comparison zones, and no zones were dominated by pollution -tolerant species. The proportion of sunfish identified as hybrids was less than 3% with no pattern between thermally influenced and non -influenced zones. Catch rates and size structures of representative important species (RIS; Largemouth Bass, Alabama Bass, Bluegill, Redbreast Sunfish, Redear Sunfish, Gizzard Shad, and Channel Catfish) indicated multiple age classes of each species throughout the lake. There were no observed differences between the thermally influenced zones and associated reference zones that would suggest negative effects to the fish populations as a result of operations at BCSS. Condition factors, and indication of fish health, were average for all RIS. No patterns of condition were observed that would indicate any negative impacts from the thermal discharge at BCSS. The 2020 data indicated that the Belews Lake fish community is balanced and indigenous and is composed mostly of indigenous species expected from a reservoir 1 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station located in the NC piedmont. Additionally, no threatened or endangered species exist in the project area that may be impacted from the thermal discharge of BCSS. Under the current thermal compliance point, the survival, reproduction, development, and growth of BIC have not been appreciably reduced due to operations. Additionally, the BCSS thermal plume has not blocked or inhibited access to any potential spawning habitat, spawning activities, or the development of early juveniles of RIS and the BIC. Consequently, the current thermal limits and BCSS operations have ensured the protection of a BIC in Belews Lake. 1 Introduction 1.1 Physical Description Belews Creek Steam Station (BCSS) is a two -unit, coal-fired electric generating plant located in the Dan River (Roanoke) drainage of Stokes County, North Carolina, approximately 15 miles northeast of Winston-Salem (Figure 1-1). The reservoir, Belews Lake, was constructed principally as a cooling water source for BCSS, providing condenser cooling water (CCW) to the station. The lake first reached full pond in 1973 after the dam was completed in 1970, with BCSS Unit 1 beginning commercial operation in August 1974, followed by Unit 2 operation in December 1975. Each 1,245.6 MW unit is cooled by CCW pumped at a maximum rate of 33.1 m3/s. Historically, BCSS has been operated as a baseload generating station; however, coal-fired generation has declined significantly in recent years. Belews Lake has a surface area of 15.63 km2 at full pond elevation (221 m-msl) and is relatively deep for a Piedmont reservoir (14.6 m mean depth). The watershed, however, is small (197 km2) and has an average drainage flow of 2.8 m3/s. Belews Lake is comprised of distinct regions, which in part relate to its principle tributaries: the West Belews Creek arm; the Belews Creek arm; and the main body of the reservoir, wherein their confluence lies (Figure 1-2). The upper portion of the West Belews Creek arm receives heated effluent from the BCSS once -through CCW system. This area is physically separated from the remainder of Belews Lake, except for a 1.5-km, man-made canal that facilitates the return of heated CCW effluent to the Belews Creek arm (Figure 1-2). Low inflows, combined with evaporative loss from the station, results in a long average retention time of 1,500 days. A make-up pumping station (located on the Dan River adjacent to Belews Lake) is used if the lake elevation drops to 220.1m-msl or below. The shoreline of Belews Lake is mostly steep, buffered primarily by undeveloped forest with sparse residential development. Much of the nutrient load from the watershed is sequestered in the upper reaches of the lake. As a result, there is a productivity gradient from "uplake" areas to "downlake" (Figure 1-2). Within the downlake region of Belews Lake, a high degree of uniformity in water quality has historically been evident (Duke Energy 2005, 2011, 2015). This is principally due to a forced circulation pattern induced by the operation of the combined 66.2-m3/s capacity of the BCSS CCW pumps. The CCW system flow rate significantly exceeds typical inflow rates from combined reservoir tributaries (estimated to average 2.8 m3/s; Cumbie 1978). Particularly during the thermally stratified portion of the year, BCSS CCW pumping effectively maintains a circulation pattern within the epilimnion of the downlake region. 2 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Moving southward from the downlake region into the Belews Creek arm, beginning at about 1 km from the BCSS CCW discharge canal, a narrowing of the lake occurs. This area has historically been termed the "midlake" region, characterized as a transitional zone situated between the reservoir's headwaters and the downlake region. In this portion of the lake, the upper part of the water column typically reflects downlake water quality as influenced by the edge of the BCSS thermal plume. However, the deeper portion of the midlake water column typically reflects water quality more like that observed in the headwater portion of the reservoir (i.e., cooler water, with greater concentrations of nutrients and suspended solids). Following the fish population collapse that occurred in the 1970s and early 1980s due to selenium loading in Belews Lake, this midlake region was considered a key indicator area in reservoir -wide assessments and was closely monitored to assess the early stages of recovery of the fish community. 3 NORTH CAROLINA CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 0 20 40 80 Kiiorme:ers Figure 1-1. Roanoke River drainage in North Carolina and Virginia, and location of Belews Lake. 4 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 3.11' Fine Hail Thermal Compliance .Point Belews Creek Steam Station CCW ❑ischa rge Canal 65 �eEews West Belews Creek Creek • Belews Creek Belews Lake Ccw Inta ccw Discharge •7i}F �'Jl;f� �} East Belews Creek Belews Dam r I -= !El esrs Cheeky =�. a lEIS' =L i cep. • 0 0.5 1 11 11 I I I o . D.75 1.5 I I wtok Aakr.�nr i� papn.8cres 2 Miles 3 Kilometers Figure 1-2. BCSS location on Belews Lake, associated tributaries, and other site features. 5 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 1.2 Thermal Permitting History Belews Creek Steam Station operates under NPDES permit no. NC0024406. The initial permit was issued by the State of North Carolina in 1970 prior to development of the Clean Water Act (CWA) and associated §316(a) requirements for a thermal variance. The initial permit granted BCSS a temperature variance that stated daily average ambient water temperatures shall not exceed 32 °C at the dam discharge as a result of BCSS operations. Subsequent permits -maintained language similar to the initial permit until 2012. In 2012, North Carolina's Division of Water Resources (DWR) issued BCSS a NPDES permit, and stated in Section A. (15.), "The thermal variance granted by the State of North Carolina terminates on expiration of the NPDES permit. Should the permittee wish a continuation of its thermal variance beyond the term of this permit, reapplication for such continuation shall be submitted in accordance with 40 CFR Part 125, Subpart H and Section 122.21 (1)(6)...The temperature analysis and the balanced and indigenous study plan shall conform to the specifications outlined in 40 CFR Part 125 Subpart H and the Environmental Protection Agency's (EPA) draft 316a Guidance Manual, dated 1977." Upon review of the 2011-2015 BCSS 316(a) report submitted to the State in 2016, North Carolina Department of Environment and Natural Resources (NCDENR) commented that the report did not satisfy the 2012 permit requirements specified in Section A. (15.). To address these comments and continue operating under a thermal variance, the 2019 NPDES permit for BCSS required in Section A. (24.) a 1-year comprehensive §316(a) Demonstration study, performed in accordance to specifications in 40 CFR Part 125 Subpart H and the EPA's 1977 draft 316(a) Guidance Manual. Also, per the requirements of the 2019 NPDES permit, a BCSS §316(a) Study Plan (Study Plan) was prepared by Duke Energy and submitted to the North Carolina Department of Environmental Quality (NCDEQ) and the U.S. Environmental Protection Agency (USEPA) on July 8, 2019 (Appendix A, Duke Energy 2019). Approval of the Study Plan was received from the NCDEQ on November 7, 2019 and studies commenced in January 2020. 1.3 Environmental Monitoring History Duke Energy has performed or sponsored environmental monitoring on Belews Lake since dam construction was completed in 1970. The initial study was performed during 1970-1977 and included three years prior to full pond, one year at full pond before station operation, and three years after the station began operation (Weiss and Anderson 1978). This study evaluated water quality and chemistry, phytoplankton, zooplankton, and benthic macroinvertebrates. The North Carolina Wildlife Resource Commission (NCWRC) surveyed the Belews Lake fishery for sport fish potential during the same time period (Van Horn 1978). By 1975, substantial declines in fish populations and recruitment became evident in lower Belews Lake, and it was determined that selenium loading from BCSS ash basin sluicing into the lake, exacerbated by the long retention time, was inhibiting fish reproduction (Harrell et al. 1978). Environmental studies were restructured to monitor effects of a perturbation related to discharge of ash basin effluent to the lake and selenium bioaccumulation during the period 1976-1985. During 6 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 1984, BCSS began dry fly -ash collection to eliminate most selenium accumulation and other trace element inputs to the ash basin. These lake recovery sampling programs evolved over time and new sampling programs were created when BCSS redirected its regulated ash basin discharge to the Dan River in October 1985. Belews Lake biota began to recover once this redirection occurred (Duke Power Company 1996). Duke Energy and NCDEQ (formally NCDENR) have acknowledged that macroinvertebrate and fish populations have substantially recovered from the selenium contamination episode during the early operations of BCSS (Duke Energy 2005; Barwick and Harrell 1997). Over the past several decades, environmental monitoring on Belews Lake has focused on water quality/chemistry, benthic macroinvertebrates, and fisheries. Water quality and water chemistry samples have been collected on at least a semi-annual basis since 1977. Annual cove rotenone surveys were performed from 1977 to 1994 to sample fish populations (Duke Power Company 1996). Electrofishing surveys began in 1983 to sample the fish community, and these surveys have continued. During 1991-2016, benthic macroinvertebrate community samples were collected. Starting in 1996, lake environmental data were submitted to NCDEQ during each NPDES permit cycle. The NCWRC has also conducted several fisheries surveys and research projects on Belews Lake over the years (Hining 2003, 2005a, 2005b; Hodges 2012). For the purposes of this report, historical data available since 2001 are provided for context with 2020 data (Duke Energy 2005, 2011, 2015). Also, extensive water quality data collected by Duke Energy in 2017 are utilized in the temperature analysis portion of the report, in conjunction with hyperspectral satellite imagery obtained in 2019 to delineate the thermal plume. 1.4 Station Operations and Weather Characteristics Station capacity factors, along with cooling water temperatures, have a direct effect on the resulting thermal discharge into the lake. When maintenance is performed or electricity demand is decreased, capacity is reduced. In 2017, the average annual net unit capacity was 39.8% for Unit 1 and 57.6% for Unit 2 (Table 1-1). In 2020, the average annual net unit capacity was 27.5% for Unit 1 and 26.8% for Unit 2 (Table 1-2). The NPDES thermal compliance discharge limit for BCSS is an ambient water temperature of 32 °C (89.6 °F) and is defined as the daily average discharge water temperature when a discharge from the lake dam occurs (No. NC0024406). Discharges from the lake dam are primarily utilized to ensure the structural integrity of the dam and maintain lake level. Thermal discharge limits were met throughout 2017 and 2020 (Table 1-3). The maximum daily average water temperature recorded at the spillway was 29.2 °C (84.6 °F) reported in July 2020 (Table 1-3). 7 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 1-1. Net capacity factors, expressed in percent (%), and monthly average discharge water temperatures (measured at CCW discharge) for BCSS during 2017. Net capacity factor Monthly average discharge temperature Unit 1 Unit 2 Station °C °F January 32.9% 32.1% 32.5% 16.7 62.0 February 10.5% 15.6% 13.1% 14.4 57.9 March 44.6% 84.3% 64.5% 21.8 71.2 April 70.5% 56.7% 63.6% 28.6 83.5 May 56.6% 69.6% 63.1% 32.0 89.6 June 79.9% 63.6% 71.7% 37.1 98.8 July 85.7% 82.4% 84.0% 41.4 106.5 August 78.9% 79.3% 79.1% 40.1 104.2 September 0% 53.4% 26.4% 29.8 85.6 October 0% 29.2% 14.5% 23.7 74.7 November 0% 46.1% 22.9% 19.0 66.2 December 17.6% 79.3% 48.5% 19.8 67.6 Average 39.8% 57.6% 48.6% 27.0 80.6 Table 1-2. Net capacity factors, expressed in percent (%), and monthly average discharge water temperatures (measured at CCW discharge) for BCSS during 2020. Net capacity factor Monthly average discharge temperature Unit 1 Unit 2 Station °C °F January 1.9% 68.2% 35.1% 16.7 62.1 February 15.3% 3.2% 9.3% 12.3 54.2 March 32.8% 17.1% 25.0% 16.6 61.8 April 3.3% 0% 1.4% 17.0 62.6 May 0% 19.1% 9.2% 20.7 69.2 June 55.0% 67.2% 61.1% 34.7 94.4 July 64.6% 76.2% 70.4% 38.5 101.3 August 68.0% 65.9% 67.0% 38.2 100.8 September 56.0% 5.3% 30.7% 31.9 89.5 October 26.9% 0% 13.2% 23.3 74.0 November 0% 0% 0% 19.1 66.4 December 6.4% 0% 3.1% 15.2 59.4 Average 27.5% 26.8% 27.1% 23.7 74.7 8 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 1-3. Number of flow releases and daily average water temperatures at spillway compliance point for BCSS during 2017 and 2020. Month Number of Minimum Daily Maximum Daily Flow Average Water Average Water Releases Temperature (°C) Temperature (°C) 2017 January 4 11.4 12.1 February 5 11.1 12.3 March 3 12.3 16.1 April 13 14.5 21.8 May 18 16.6 22.6 June 3 19.8 25.6 July 0 - August 0 - September 0 - October 0 - November 0 - December 0 - 2020 January 22 11.0 13.1 February 21 10.3 11.7 March 11 11.2 15.1 April 14 14.1 16.8 May 20 15.7 19.4 June 9 21.0 24.5 July 5 25.8 29.2 August 21 19.0 31.4 September 6 19.7 24.4 October 10 21.5 23.5 November 16 15.9 19.3 December 20 10.4 15.5 Meteorological forces can exert significant influences, both directly and indirectly, on the physical, chemical, and biological characteristics of aquatic ecosystems, and documentation of local and regional meteorology can often provide insight into the spatial and temporal dynamics of these characteristics (Wetzel 2001). Two important meteorological parameters are air temperature and precipitation, and data for these two variables were obtained from two local area monitoring stations: National Weather Service (NWS) monitoring station in Greensboro, NC (air temperature) and United States Geological Survey Station (USGS) station (precipitation) near Pine Hall, NC. Air temperatures influence variability in a waterbody's thermal regime via seasonal water column heating and cooling. Air temperatures recorded at the NWS station during 2017 and 2020 were generally above average compared to data collected since 2010 (Figure 1-3). Notably higher than average air temperatures were recorded in February 2017, March 2017, April 2017, March 2020, and November 2020 with monthly means 2.0-6.0 °C greater than the average (Figure 1-3). Monthly air temperatures were notably below average in December 2017 and May 2020 with monthly means 1.7 °C and 3.9 °C below average, respectively. 9 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Temperature VC) 35 30 25 20 15 10 0201D-2019 Avg 2017 —111-2020 k # \A ��As. `,�a ft �` , a Figure 1-3. Mean monthly air temperatures recorded near Belews Lake, North Carolina during current study period compared to 2010-2019 average. Precipitation affects hydrologic characteristics in aquatic ecosystems by controlling water volume, inflow rates, and water column mixing. This hydrodynamic influence can be additionally magnified or modified by reservoir outflow characteristics, resulting in variations in spatial and temporal water quality and biological regimes. In addition to influencing hydrologic and hydraulic characteristics, precipitation can impact water quality by direct chemical loading associated with atmospheric chemistry or indirectly via constituent loading associated with watershed runoff. The rainfall total of 116.3 cm recorded in 2017 and 152.8 cm in 2020 was above the historical average of 107.9 cm (Figure 1-4). Monthly precipitation totals during 2017 and 2020 were variable and ranged from 7.6 cm below average in July 2020 to 14.6 cm above average in April 2017 (Figure 1-5). These rainfall patterns directly affected the hydrologic inflow and discharge of the reservoir. 10 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Precipitation (cm) 180 160 140 120 100 80 60 40 20 0 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 Figure 1-4. Total annual precipitation recorded at Belews Lake during current study period compared to 2010- 2019 average (horizontal line). Historical data collected from USGS monitoring station at Pine Hall, NC. Precipitation (cm) 30 25 20 15 10 O 2010-2019 Avg t 2017 f 2020 �a°%). e��• etJ �etr t Q o��y� ��et°sec �vec �`vec P he,Qxe 0('+°�e Oece Figure 1-5. Total monthly precipitation recorded at Belews Lake during 2017 and 2020 compared to 2010-2019 monthly averages. Historical data collected from USGS monitoring station at Pine Hall, NC. 11 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 2 Study Goals and Objectives In accordance with NPDES permit no. NC0024406, Section A. (24.) and the agency approved Study Plan (Appendix A), Duke Energy conducted an initial and comprehensive one-year demonstration study on Belews Lake in 2020 to support a thermal variance request under §316(a) of the CWA. The two primary goals accomplished during this study were: (1) the completion of a temperature analysis, and (2) the demonstration of a self-sustaining, balanced and indigenous biological population (BIP) or community (BIC) of fish and shellfish in Belews Lake. Data obtained for the temperature analysis were used to describe the spatial extent of the BCSS thermal plume in Belews Lake, both horizontally and vertically. Characterization of the plume throughout the water column was used to assess migration barriers to fish movement as it relates to thermal tolerances of certain fish species. As detailed in the Study Plan, several study components were proposed to make a BIC assessment of Belews Lake, including a temperature analysis, limnological sampling, fisheries sampling, freshwater mussel surveys, identification of habitat formers, other vertebrate wildlife observations, and identification of any rare, threatened or endangered species. Also, emphasis was given to the study of Representative Important Species (RIS), which were incorporated in the assessment to indicate a BIC exists within Belews Lake. The following species/species groups were approved by the NCDEQ as RIS in the Study Plan: Gizzard Shad Dorosoma cepedianum, Channel Catfish lctalurus punctatus, Redbreast Sunfish Lepomis auritus, Bluegill Lepomis macrochirus, Redear Sunfish Lepomis microlophus, Alabama Bass Micropterus henshalli, Largemouth Bass Micropterus salmoides, and native freshwater mussels. Although Alabama Bass and Channel Catfish are not considered to be native, or "indigenous" to the Roanoke River basin, the subpart of 40 CFR Part 125 states that a BIC, "...may include ...species whose presence or abundance results from substantial, irreversible environmental modifications." Coutant (2013) suggested that an example of this, "would include reservoir species not native to the area before impoundment" (e.g., introduced game and nongame fish). If available, additional reference locations are valuable in 316(a) demonstrations (Coutant 2013). Mayo Reservoir is an impoundment of Mayo Creek in Person County, North Carolina, approximately 65 miles ENE of Belews Lake created to supply make-up water to Mayo Steam Electric Plant (MP). Although Mayo Reservoir is smaller and shallower than Belews Lake, it is a good candidate as a reference lake because of reasons such as little to zero thermal input, has similar productivity and nutrient load (low to moderate), is in the same river basin as Belews Lake, has a similarly long retention time, and was recently found to have a "relatively balanced and stable fish community" by the North Carolina Division of Water Resources (NCDWR) in 2018. Therefore, fish community reference data from Mayo Reservoir were compared with data from Belews Lake during the study. Fish and shellfish (freshwater mussels) data were collected lake -wide and were evaluated against the following four primary BIC criteria defined in 40 CFR 125.71. The four criteria state that BICs are biotic communities typically characterized by: a. Having diversity and representative trophic levels within expectations, b. The ability to self -sustain through successful reproduction and recruitment over seasonal changes, 12 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station c. Having adequate food items, and d. A lack of domination by pollution tolerant species. 3 Methods For the purposes of this report and the assessment of Belews Lake biological communities, it was necessary to establish zones of study as they relate to thermal plume characteristics. This included the following six zones, setup alphabetically with respect to water temperatures warmest at Zone A and coolest at Zone F (Figure 3-1): Zone A— upper West Belews Creek arm, adjacent to the immediate CCW thermal discharge and thermal plume; Zone B — upper West Belews Creek arm, distant from the immediate CCW discharge, but within the thermal plume; Zone C — lower Belews Creek arm in proximity to the CCW discharge canal exit and within the thermal plume; Zone D — lower West Belews Creek arm, near the CCW intake and within the outer thermal plume; Zone E — main lake area near Belews Dam, downlake and within the outer thermal plume; and, Zone F — lower East Belews Creek arm, distant and outside of the thermal plume. 13 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 410.._0(4? Ly-Th Jr-7 .1 3� Sf ciNsr Sampling Locations • WO Profiles &WC WO Prorile5, WC & 2O17 Loggers 0. WC Only ▪ WO Prolli;es aril 2417 Loggers ▪ PVile Only A 0 0.375 0,76 1.5 Miles rr r r� �.. T ri.LI 8 0.5 'I 2 FKilorneters Figure 3-1. Limnological (water quality and chemistry) sampling locations and zones in Belews Lake. 14 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 3.1 Temperature Analysis The Belews Lake temperature analysis included three aspects: (1) compilation and review of station operations data, (2) intensive in -situ monitoring, and (3) thermal plume characterization. Operations data obtained included net generation totals from 2017 and 2020 (Tables 1-1 and 1-2). Additionally, continuous temperature data was collected at the following locations: Zone A, Zone C, Zone D, Zone E, and Zone F. A rigorous temperature sampling program in Belews Lake was conducted in 2017 (January —December) in anticipation of the upcoming temperature analysis requirement within the 2020 316(a) demonstration. Monthly water quality profiles (measurements from surface to bottom at 1-m intervals) were collected at 12 locations in Belews Lake, along with continuous in -situ temperature profile loggers (Onset®, Bourne, Massachusetts) at five of the locations (Figure 3-1). These continuous loggers recorded hourly temperature data and were deployed from the surface down to 20 m (or lake bottom, whichever was less) at two -meter intervals, except for location 416.0 near the dam. An internal temperature logger provided surface water temperature data for location 419.2 (Zone F). Data were analyzed using time -series graphs and contour plots to display seasonal conditions and document thermal stratification in Belews Lake. Surfer Software (Golden Software® 2017) and SigmaPlot® were used for developing contour plots for water temperature profiles. Spatial analysis of the surface thermal plume in Belews Lake during winter and summer extreme scenarios were produced from archived satellite imagery. Satelytics, Inc. (Perrysburg, Ohio) provided the images from Landsat 7 Band 6 (thermal; X = 10.4-12.5 µm) satellites, with thermal resolution reported at 1 °C. Water temperature data was calculated from a patent -applied algorithm derived from hyperspectral Landsat satellite imagery data measured at a density of approximately 5 samples per acre. Based on historical meteorology, hydrological, and operational conditions (full generation), January 4, 2018 was selected for the extreme winter condition, while July 27, 2002 was selected for the extreme summer condition. In both cases, data provided in the thermal plume maps represent actual conditions or temperatures as measured by the Landsat 7 Band 6 satellites. The resulting imagery data were imported into ArcGIS Pro to produce thermal plume maps for both extreme scenarios. The combination of monthly profiles, continuous temperature loggers, and satellite imagery were all used in the temperature analysis to determine the vertical, horizontal, and spatial extent of the thermal plume under extreme conditions. 3.2 Limnology Limnological data are used to characterize the environmental conditions of a waterbody. These data help provide an understanding of the basic productivity and biological changes within aquatic ecosystems, examples of which could include chronic changes in population dynamics or acute changes in fish behavior. Additionally, the collection and analysis of water quality and chemistry data can be used to evaluate station operation, watershed, and meteorological effects on the aquatic ecosystem. Field parameter measurements of temperature, dissolved oxygen (DO), pH, and specific conductivity were collected in situ at each location once per quarter in conjunction with fisheries sampling. Field parameters were collected with a Hydrolab data sonde (OTT Hydromet, Loveland, Colorado) or an Aqua TROLL 600 multiparameter sonde (In -Situ, Fort Collins, Colorado) starting at the lake surface (0.3 m) and continuing at one -meter intervals to 10 m, then two -meter intervals to the lake bottom. Pre- and 15 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station post -calibration procedures associated with operation of the sondes were documented in electronic format. Hydrolab data were captured and stored electronically and converted to spreadsheet format following data validation. All Hydrolab data were uploaded to EQuIS Professional (Earthsoft, Pensacola, Florida) following data validation. Aqua TROLL data were captured and stored in an electronic format using Earthsoft EDGE, then uploaded to EQuIS Professional following data validation. Water chemistry samples for laboratory analysis were collected semi-annually in 2020 with a peristaltic pump, or by direct grab at the surface (0.3 m). Samples not requiring filtration were discharged directly into high -density polyethylene (HDPE) or polyethylene terephthalate (PET) sample bottles. Dissolved - fraction samples were field -filtered with a 0.45-µm in -line filter capsule and peristaltic pump. Filter capsules were pre -rinsed by running a minimum of 500 mL of sample water through the filter prior to filling the the sample bottles. Filtered and unfiltered sample bottles were pre -acidified where applicable. Chlorophyll -a and nutrient samples were collected from the measured photic zone using a depth integrated sampler. The photic zone was determined as twice the secchi depth, which was measured by lowering a secchi disk to extinction prior to sampling. Samples were stored on ice and in the dark immediately following collection to minimize the potential for physical, chemical, and/or microbial transformation. Sampling and sample transport followed an established Chain of Custody process. Laboratory analytical methods, reporting limits, and sample preservation techniques are included in Table 3-1. The analytical parameters included in this study (excluding low-level mercury and chlorophyll -a) were analyzed by a NCDWR certified laboratory, primarily the Duke Energy Analytical Laboratory in Huntersville, NC (Certification #248). Laboratory analysis for low-level mercury samples were performed by Shealy Environmental labs in West Columbia, SC. Chlorophyll -a samples were analyzed by ETT Environmental laboratories in Greer, SC. 16 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 3-1. Analytical methods and reporting limits for parameters monitored in Belews Lake in 2020. Parameter Method (EPA/APHA) Preservation Reporting Limit Temperature Thermistor, APHA 2550 In situ 0.1 °C Luminescent (LDO) cell, Oxygen, Dissolved In situ 0.1 mg/L ASTM D888-09-C pH Glass Electrode, EPA 150.2 In situ 0.1 unit Conductance, Specific Thermistor, EPA 120.1 In situ 1 µS/cm3 Chlorophyll -a SM10200H 6 °C 2.0 µg/L Alkalinity SM 2320E 6 °C 5 mg/L Phosphorus, Total Colorimetric, EPA 365.1 0.5% H25O4 0.005 mg/L Orthophosphorus Colorimetric, EPA 365.1 6 °C 0.005 mg/L Ammonia Colorimetric, EPA 350.1 0.5% H2SO4 0.02 mg/L Nitrate + Nitrite Colorimetric, EPA 353.2 0.5% H2SO4 0.01 mg/L Total Dissolved Solids SM 2540C 6 °C 25 mg/L Total Kjeldahl Nitrogen Colorimetric, EPA 351.2 0.5% H2SO4 0.1 mg/L Total Organic Carbon SM 5310E 0.5% H2SO4 0.2 mg/L 'Calcium ICP, EPA 200.7 0.5% HNO3 0.01 mg/L 'Magnesium Atomic Emission/ICP, EPA 200.7 0.5% HNO3 0.005 mg/L 'Mercury, Low -Level EPA 1631E Clean cooler 0.5 ng/L Chloride Ion Chromatography, EPA 300.0 6 °C 0.1 mg/L Sulfate Ion Chromatography, EPA 300.0 6 °C 0.1 mg/L Secchi Hutchinson (1975) N/A N/A Turbidity Turbidimetric, SM2130-2011 6 °C 0.05 NTU Total Hardness Calculation, EPA 200.7 N/A N/A 'Arsenic, Total ICP Mass Spectroscopy, EPA 200.8 0.5% HNO3 1.0 µg/L 'Copper, Total ICP Mass Spectroscopy, EPA 200.8 0.5% HNO3 1.0 µg/L 'Selenium, Total ICP Mass Spectroscopy, EPA 200.8 0.5% HNO3 1.0 µg/L 'Zinc, Total ICP Mass Spectroscopy, EPA 200.8 0.5% HNO3 5 µg/L 'Total Metals, Low -Level Mercury, and Total Hardness were only collected at Zone D and Zone F. Water quality and analytical chemistry data were subjected to various numerical and graphical techniques to evaluate spatial and temporal trends within the lake, interrelationships among constituents, and the potential effect on lake biota. Data were evaluated using seasonal comparisons between sampling locations and historical comparisons across the lake. Contour plots for water temperature and dissolved oxygen were developed using SigmaPlot® software. Data within each lake zone were compared to lake -wide values for context. Analytical results reported to be equal to or less than the method reporting limit were evaluated at the reporting limit for purposes of numerical and statistical assessments. Real Statistics (Real Statistics Resource Pack 2019) and SigmaPlot® were utilized for developing time - series, box and whisker, contour plots, and other statistical analyses. Time series and box and whisker 17 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station plots were utilized to compare surface values (0.3-m samples). Contour plots for water temperature and dissolved oxygen were developed using SigmaPlot® software. Statistical analysis of surface temperatures by season from continuous temperature loggers deployed in 2017 was completed utilizing the Tukey-Kramer multiple comparison method to verify that zone groupings appropriately represent thermally impacted and background areas within the lake, (i.e., statistically significant at the p<0.05 significance level) (Appendix B). The availability of locations outside of the CCW thermal discharge in the uplake reaches of the lake (Zone F) and in the downlake area near Belews Dam (Zones D and E) allowed for comparison with areas thermally influenced by the BCSS CCW discharge (Zones A, B, and C) to assess potential impacts (Figure 3-1). The 2020 data were compared to historical data to evaluate recent changes in the waterbody and detect long-term trends (Appendix C). For the purposes of this report, historical water quality and analytical data included the years 2001-2019. 3.3 Planktonic Community Sampling of Belews Lake plankton communities was not performed in 2020 as part of the demonstration study. Instead, a plankton rationale or narrative is provided. The narrative describes plankton communities within the framework of the Belews Lake BIC and their categorization as a low potential impact (LPI) biotic category. The narrative includes an assessment of plankton as LPI using scientific literature and historical planktonic data collected in Belews Lake (Coutant 2013; Duke Energy 2005, 2011, 2015; Duke Power Company 1996, 2000; Weiss and Anderson 1978). 3.4 Habitat Formers Habitat formers are defined as assemblages of living or once living plants and animals with relatively sessile life stages that may have aggregated distributions. Such assemblages may function as living substrate for epibiota, direct or indirect food sources, biological mechanisms for sediment stabilization, nutrient cycling pathways or traps, and/or spawning or rearing habitats (EPA 1977). In Belews Lake, habitat formers are primarily made up of aquatic plant communities, including those classified as submerged, floating leaf, free-floating, and emergent. Aquatic plant communities and species distribution were assessed through an intensive survey of the littoral zone (0-4.6 m) of Belews Lake in September 2020. This time period corresponds to the end of the growing season when aquatic plants would be at their maximum biomass and distribution. This survey covered approximately 475 hectares or 31% of the lake. Aquatic plant communities were assessed using a meandering shoreline survey of 1,252 points spaced roughly 80 m apart to determine species presence/absences (Figure 3-2, Madsen and Wersal 2012). Sampling of submersed plants was accomplished using hydroacoustic technology to identify any existing submersed biomass. Once detected, submersed populations were sampled using a double -sided rake for species identification. Vegetation density of submersed plants was also assessed at each point using a 0 to 4 rating scale (0 = no vegetation present; 1 = vegetation present at low densities, < 25% coverage; 2 = vegetation present at moderate densities, 25-50% coverage; 3 = vegetation present at moderate to high densities, 50-75% coverage; and 4 = vegetation present at extremely high densities, >75% coverage). Emergent and floating leaf species directly adjacent to sampling points were assessed visually, and given a density designation of 0 to 4 based on the continuity of the existing bed (0 = no plants directly adjacent; 1= trace amounts of vegetation present, no continuity; 2 = moderate amounts of vegetation present, not extending to next sampling location; 3 = moderate to high amounts of vegetation present, extending >50% to next sampling location; and 4 = 18 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station high densities of vegetation present, completely continuous to next sampling point). Areas of emergent or floating vegetation with >3 species were designated as an "diverse wetland community". As discussed above, results were summarized based on designated zones of thermal influence, derived from preliminary summer (growing season) temperature data (Figure 3-1). Points Sampled: 1,172 Littoral Area Sampled: 475 ha of Total Lake Area: 31% Avg. Depth: 3.05 m Data Collection Date(s): Sep 14-16, 2020 Belews Lake Aquatic Vegetation ° Sampling Locations 0 1.25 2.5 l i i i l 5 Kilometers Figure 3-2. Aquatic plant species presence/absence sample locations in Belews Lake. 3.5 Benthic Macroinvertebrate Community Sampling of Belews Lake benthic macroinvertebrate communities (insects) was not performed in 2020 as part of the demonstration study. Instead, a macroinvertebrate rationale or narrative is provided. The narrative describes macroinvertebrate communities within the framework of the Belews Lake BIC and their categorization as an LPI biotic category. It includes an assessment of macroinvertebrates as LPI using scientific literature and historical macroinvertebrate data collected in Belews Lake (Coutant 2013; Duke Energy 2005, 2011, 2015; Duke Power Company 1996, 2000; Weiss and Anderson 1978). 3.5.1 Mussel Community Qualitative mussel surveys were conducted in August 2020 to document the presence/absence of freshwater mussels, determine assemblage richness, and provide the relative abundance (catch-per- 19 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station unit -effort, CPUE) of each species. Methods followed Duke Energy procedures (on file with NCDEQ) with each survey encompassing 4.0 person -hours of search time or at least a distance of 200 m. Mussels were collected visually and tactilely (grubbing) of all habitats within the survey area that were less than 4 m in depth and placed in mesh bags. After the survey, the bags were brought to the surface and mussels were identified to species. Lengths of 50 individuals (if available) from a representative sample of each species at each site were measured to the nearest mm. The length of the mussel was measured, with calipers, along the ventral margin from anterior to posterior end. Mussels were replaced at the approximate location from which they were removed. Two timed surveys were conducted within six study zones (12 total surveys, Figure 3-3), and all native mussel species collected were considered RIS. 20 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Belews Creek Steam Station Sampling Locations • Electrofishing • Gillnetting Mussel Survey 0 0.75 1.5 3 Kilometers Figure 3-3. Fish and mussel sampling locations, and zones of thermal influence in Belews Lake. 21 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 3.6 Fish Community 3.6.1 Fish Community Assessment Electrofishing surveys were conducted in Belews Lake during January, April, July, and October 2020. Four shoreline transects, each consisting of approximately 1,000 seconds of shock time, were surveyed in the six zones of Belews Lake (Figure 3-3). Transects included habitats representative of those typically found in Belews Lake and were selected such that similar habitats were sampled in all zones. All sampling was conducted during daylight. Surface water temperature, dissolved oxygen, pH, and specific conductance were measured at each transect with a calibrated meter. Electrofishing transects paralleled the shoreline and total shock time was recorded. Stunned fish were collected by two netters (one netter was used during April, July, and October 2020 due to the Covid-19 pandemic) and held in a live well until the transect was completed. Fish were identified to species, enumerated, and for each transect a subsample of each species were measured for total length (mm) and weighed to the nearest gram. Experimental gill nets were deployed concurrently with each quarterly electrofishing survey. Gill nets were 2.4 m tall and 30.5 m long with four incremental 7.6-m panels (2.5, 5.1, 7.6, and 10.2- cm monofilament stretch mesh). Two gill nets were set in each zone for two net nights, totaling four net -- nights every quarter. Every study location had three or four potential gill net locations (Figure 3-3), two of which were randomly selected each night. Nets were set on lake bottom approximately perpendicular to the shoreline with the small mesh nearest to shore. After each net night the fish were removed. Processing of the fish was completed using the same methods described for electrofishing. Data collected during the spring electrofishing surveys were used to assess the balanced and indigenous nature of the Belews Lake fish community and provide information relative to the potential thermal influence of BCSS. This season, when surface water temperatures should be between 15 and 23 °C, is the standard time for sampling using this technique (Miranda and Boxrucker 2009). The assessment included spatial comparisons of species pollution tolerance, trophic guild, and hybrid complexes. 3.6.2 RIS Population Assessment The relative abundance and length distributions of centrarchid RIS was determined from the spring electrofishing survey. Additionally, the gill nets were included in the assessment specifically to target Gizzard Shad and Channel Catfish, and similar to electrofishing, a standardized sampling timing has been identified for using gill nets for these species. The fall gill netting survey, when water temperatures were expected to be below 20 °C, was used to assess the relative abundance and length distribution of these two species (Miranda and Boxrucker 2009). Mean catch -per -unit -effort (CPUE) was calculated for each zone along with 90% confidence intervals, and comparisons were made among zones using a one-way ANOVA with a Bonferroni correction for multiple comparisons. For all comparisons, a was maintained at 0.1. Length -frequencies were graphed and compared among zones. Data collected during the fall electrofishing and gill net surveys were used in conjunction to assess the condition (relative weight) of each RIS among zones. A standard weight equation has not been published for Redbreast Sunfish, however an equation has been created from data collected by Duke Energy (unpublished data). 22 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 3.6.3 Fish Community Similarities Differences of fish communities among zones were assessed using Primer 7 (Primer-e, New Zealand). Square root transformed CPUE data were grouped with Bray -Curtis similarities and plotted using non - metric multi -dimensional scaling. Data used included both the standardized spring electrofishing survey and the standardized fall gill net survey. Additionally, differences among zones were assessed using analysis of similarities (ANOSIM). Although multiple comparisons were made between zones, no correction was made to reduce the Type I error rate because the low sample size would make the new significance level unachievable. For the purposes of this analysis, only pairs with a significance level <0.10 and a sample statistic (R) >0.50 were considered meaningful. 3.6.4 Seasonal RIS Distributions Data collected outside of the above specified seasons, specifically winter and summer, were utilized to assess distributions of RIS among zones during times when the thermal influences from BCSS would be the most extreme. 3.6.5 Reference Lake Quarterly fisheries surveys were conducted on Mayo Reservoir using the same gear types and techniques as on Belews Lake. Mayo Reservoir does not have a thermal influence, and as such, only two electrofishing transects along with four gill net -nights of effort were conducted in each of three zones of the lake (Figure 3-4). Data collected from Mayo Reservoir were compared to those collected from Belews Lake. Specific indices compared between lakes included pollution tolerant species percentages, species trophic level percentages, species richness, and Shannon Diversity Index. In addition, RIS population assessment metrics (i.e., CPUE, size structure, and condition) were calculated for Mayo Reservoir. 23 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Zone B Mayo Station Zone E Zone G Sampling Locations • Gillnetting Only • Electrofishing and Gillnetting 0 0.4 0.8 1.6 Miles i II ( I I I I I I 0 0.5 1 2 Kilometers Figure 3-4. Fish sampling locations and zones in Mayo Reservoir. 24 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 3.7 Other Vertebrate Wildlife Wildlife observations associated with the Belews 316(a) studies were conducted for aquatic wildlife species or species that use Belews Lake for activities such as foraging or other life functions. A one -hour stationary observation was conducted during the 2020 late summer period in the heated effluent discharge arm. This observation was supplemented with wildlife observations during other field activities in the discharge arm in 2020 (e.g., habitat formers survey). 3.8 Endangered Species The USFWS Information for Planning and Construction (IPaC) database was accessed to determine whether any federally -listed species could be present near BCSS. Information regarding the protected and federally listed species were obtained via the United States Fish and Wildlife Service's (USFWS) Forsyth County, Guilford County, Rockingham County, Stokes County, NC county -wide lists (USFWS 2018), as well as site knowledge. 4 Results and Discussion 4.1 Temperature Analysis 4.1.1 In -Situ Thermal Monitoring Analysis of in -situ thermal monitoring data during 2017 revealed temporal (seasonal and annual) and spatial (horizontal and vertical) variations in water temperatures typical of other relatively deep reservoirs (with similar climate and morphometry), in the Southeastern United States. Water temperatures in southeastern reservoirs are influenced by physical factors such as watershed inflows, thermal inputs from industrial discharges, incident solar thermal energy, water depth, clarity, and retention time. These reservoirs are characterized by an annual warming period (spring —summer) in which vertical thermal stratification develops, and an annual cooling period (fall —winter) where deterioration of stratification leads to a single homogenous mixing period within the lake (Wetzel 2001). Accordingly, Belews Lake is classified as monomictic (Wetzel 2001). During the 2017 monitoring period, temperature loggers measured hourly water temperatures in five zones across the lake (i.e., Zones A, C, D, E, & F). The surface water temperatures at Zone A ranged from 11.4 °C to 41.2 °C with a mean of 25.6 °C. The water temperatures at Zone C exhibited means of 24.7 °C and ranged from 11.7 °C to 38.2 °C during 2017. Similarly, surface temperatures within Zone E yielded a mean of 21.9 °C and ranged from 10.9 °C to 35.5 °C. The surface temperatures at Zone D ranged from 12.8 °C to 34.4 °C with a mean of 22.5 °C. The surface temperatures at Zones C were slightly warmer than Zones D and E, which is expected due to the proximity to the CCW discharge. As a means of comparison, surface temperatures collected uplake in Zone F had a mean water temperature of 21.0 °C and ranged from 5.3 °C to 34.1 °C. Thus, the mean water temperature at Zone F is similar to the means observed in Zones D and E. The comparison as whole revealed that there is relatively small spatial variability in surface temperatures within the main lake in Zones D, E, and F (Figure 4-1). 25 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Surface Water Temperature (°C) 45 40 35 30 25 20 15 10 5 0 T x 1 1 x 1 T x 1 1 x 1 Zone A Zone C Zone D Zone E Zone F Figure 4-1. Surface water temperatures from temperature loggers from five zones in Belews Lake. Data cover the 2017 monitoring period. For the box plots, the line represents the median, mark "x" represents the mean, boxes depict 25th and 75th percentiles, and whiskers depict 10th and 90th percentiles. Thermal stratification in Belews Lake begins coincidental with post -winter atmospheric heating, typically in late March or early April. Analysis of historical thermal data for Belews Lake has illustrated that temporal and spatial variations in water temperatures are consistent with those found in other southeastern reservoirs. General patterns of well -mixed winter conditions and pronounced stratified summer conditions emerge each year due to the monomictic nature of the reservoir (Duke Energy 2015). The pronounced stratification pattern in Belews Lake continued during the 2017 monitoring period, largely influenced by atmospheric conditions and a relatively long retention time (Figures 4-2, 4-3, 4-4, 4-5). 26 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 40 38 36 34 32 30 28 26 24 22 20 18 16 14 12 10 E:,, 8 6 4 2 0 Figure 4-2. Isotherm plots for main lake (top) and discharge arm (bottom) areas from monthly water temperature profiles in Belews Lake. Data cover the 2017 monitoring period. Temperatures are in degrees Celsius. 27 20 18 16 14 12 10 8 iheri arum imnew.e Apr CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 471 suc ITS May a "A a+... o.mu: ]rrrs-km Sm.. N.n . ii.m. ,> tin fs 2. 2. � 2013 SRI 1114 minurk Jun [wwe. wma... oxniur a Y4 Si Figure 4-3. Isotherm plots for main lake (top) and discharge arm (bottom) areas from monthly water temperature profiles on Belews Lake. Data cover the 2017 monitoring period. Temperatures are in degrees Celsius. 28 40 38 36 34 32 30 a- 28 26 24 22 20 18 16 14 10 8 6 av 4,� 2 0 arr.,, ovne,r,w Dan�w; ow®1.1,11kla.s an i.1 7711 .1.,mironew..•annn> 1.1 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 1,1...111.11111 Sep Sep Figure 4-4. Isotherm plots for main lake (top) and discharge arm (bottom) areas from monthly water temperature profiles on Belews Lake. Data cover the 2017 monitoring period. Temperatures are in degrees Celsius. 29 40 38 36 34 32 30 28 18 16 14 8 4 2 11-5 2,0 4,8 - •t?7 .4 x i. Oct Rl r Y L'-'4 -' • , ]*.n.Item... Pin I ,e a,.ia`O.wam. r Oct ��ty.ppypn gy,. P,. IN) .718 ,>s 1:44.+4414rr Sawa �sn.ni CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 21111 RP 94.0,6 L:.gnn "ftwoui Nov i....k............i....�....a....i....i....i,....-� ...u.. ,h awro w. INsIAL r+an, 14 no aqa •+g.2 a06 Dec 00.444 4.464140Aw.,.4,) Figure 4-5. Isotherm plots for main lake (top) and discharge arm (bottom) areas from monthly water temperature profiles on Belews Lake. Data cover the 2017 monitoring period. Temperatures are in degrees Celsius. 30 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Temperature data from 2017 show water temperatures decreasing more rapidly with less distance away from the CCW discharge during the fall/winter months when compared to the summer months (Figures 4-3 and 4-4), a function of convective mixing driven by cooler atmospheric temperatures. It is evident that winter plume geometry and its extent is dictated by plant operations and weather conditions (Figures 4-2, 4-3, 4-4, and 4-5). Both summer and winter temperature profiles in 2017 reflected warming of the upper water column due to the buoyant nature of the thermal plume from BCSS at the discharge canal exit (Zone C) and to a lesser extent at the uplake area (Zone F), which is consistent with historical water temperature profiles (Duke Energy 2015). Therefore, the 2017 winter season data indicates that lake conditions would not exhibit temperatures that would have an adverse impact on the lentic community in Belews Lake in terms of causing mortality, including avoidance, or limiting reproductive success. The temperature analysis revealed that Belews Lake and a major portion of the discharge arm near BCSS (not accessible by the public) maintains temperatures fully adequate to support biological communities under adverse lake -level and weather conditions during the year. Moreover, these conditions provide an adequate zone of passage for aquatic life. Since BCSS generation is highest during the summer months, it is likely that higher water temperatures will occur during periods of peak power demand. Evidence of this can be observed in the discharge arm near BCSS during the summer months when power demand is higher, adding warmer water to the area closest to the CCW discharge (Figures 4-3 and 4-4). The thermal isotherm figures derived from monthly profiles in 2017 show that the difference in epilimnion temperatures between the area immediate of the discharge canal (410.0) and main lake are minimal during the summer months (Figure 4-4). In July 2017, the warmest epilimnion temperatures of 36 °C were observed near the confluence of the BCSS discharge canal. However, these high temperatures were localized to a small area immediate of the discharge canal, while the rest of the main lake exhibited an epilimnion depth of 5-m at 32 °C across the lake. Thus, these summer profile data demonstrate that the operations of BCSS assure the propagation and protection of the BIC, as represented by the RIS that could reside near the BCSS thermal discharge. 4.1.2 Satellite Imagery Satellite imagery was used to evaluate the thermal plume characteristics during extreme summer and extreme winter events. The extreme summer event was used to examine the unusual circumstances of high air temperatures and low lake -level that occurred on July 27, 2002. On this date, the average air temperature was 25.6 °C and the high temperature was 31.7 °C (The Weather Company (TWC) 2020). The summer months of 2002 were characterized as a severe drought year which experienced a total rainfall of 0.3 cm between the months of May and September (TWC 2020). Due to drought conditions, lake level was approximately 6.5-ft below full -pond, which was near the peak of 7-ft below full -pond for the 2002 drought year. Generation at BCSS was also high, which yielded CCW discharge water temperatures greater than 40 °C from June to August 2002. This scenario captured hot and dry summer conditions combined with high power generation (peak CCW thermal load) at Belews Lake. The winter extreme scenario was examined to evaluate thermal plume characteristics under very cold air temperatures during high power generation. Such conditions were present at Belews Lake on January 4th, 2018 when the average air temperature was 8.9 °C and the low temperature was 5.7 °C (TWC 2020). The average air temperature in January 2018 was 2.1 °C and power generation at BCSS was operation at an average plant net capacity factor of 81% during January 2018. Additionally, precipitation totals were below average in January 2018, with a total of 4.75 cm. 31 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Belews Creek Steam Station r'? --vr r, ` _ Summer Flume Temperature (C) Scale 0 0.425 0.85 II I 0 0.75 1.5 Figure 4-6. Belews Lake, extreme summer thermal plume map (July 27, 2002). - High : 31.8+ Low : 22.1 A 1.7 Miles i I I 3 Kilometers 32 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Belews Creek Steam Station Winter Plume Temperature (C) Scale 0 0.425 0.85 ti 'I II ' 0 0.75 1.5 High : 16.2+ Low : 0.5 1.7 Miles I � H 3 Kilometers Figure 4-7. Belews Lake, extreme winter thermal plume map (January 4, 2018). 33 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station The thermal plume in Belews Lake was delineated as 2.8 °C above the ambient temperature (EPA 1988) in Zone F (i.e., 419.2). Satellite imagery for extreme summer and winter scenarios show a larger thermal plume during the winter extreme scenario (Figures 4-6 and 4-7). The satellite imagery of the summer plume indicated that the warmest water was confined to the discharge area near Zones A and B as well as a portion of the main lake in Zones C and D (Figure 4-6). This is expected given the proximity to the plant and the relatively shallow depth of the discharge arm. However, the thermal plume extent encompassed Zones A, B, and C as well as a portion of Zone D. The summer plume map also shows cooler water temperatures as the discharge arm flows into the main lake from the discharge canal (Figure 4-6). Data from temperature loggers in July and August of 2017 support this assertion, revealing that water cools 2.5-3.0 °C between the BCSS CCW discharge (Zone A) and discharge canal exit (Zone C). The summer plume map also indicates that the warmest water is primarily localized downlake, mainly between the discharge canal confluence and plant intake (Figure 4-6). Isotherm plots for time periods near the extreme summer scenario show that a combination of severe drought conditions, high generation, and high air temperatures could result in a 12 m epilimnion with temperatures >32 °C (Figure 4-8). 225 220 418 416 Sampling Locations 410 419.3 419.2 405 215- 195- 190- 185- 28 74J 16 LF LV - 32 16 August 27, 2002 (severe drought) Average Station Temperature (°C} Discharge Temp: 41.40°C 180- 1175 .. I I I I I I I I I I I I I' 1 1! 1 I 1 1 1 1 I 1 1 1 1 1 1 1 1 1 I 1 1 1 1 I 1 1 1 1 I 1 1 1 1 I 1 1 1 1 I 1 1 1 I 1 1 -8 -6 -4 -2 0 2 4 6 8 10 12 ['Mane from Belews Dam (km) 14 16 Figure 4-8. Near summer extreme scenario isotherm plot on Belews Lake. Plot generated from water temperature profile data measured on August 27, 2002. Temperatures in degrees Celsius. ■ 335 — 33 — 32 i 30 29 2$ 2 26 a%2a4 a}21 17 18 1154 13 111211 10 9 56 3 2 1 0 The winter scenario plume shows the warmest water primarily resides in the discharge arm (Zones A and B) and in the main lake near the discharge canal exit (Zones C and D) (Figure 4-7). The spatial distribution of the winter thermal plume is confined to the majority of the discharge arm (Zones A & B) 34 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station and most of the main lake (Zones C, D, and E). This finding is characteristic of a reservoir with thermal inputs under typical winter conditions, which are subject to more diffusion and subsurface mixing in the winter months. Isotherm data from the near extreme winter scenario demonstrate that only a small portion of the main lake near the discharge canal exit (410.0) experiences a 16 °C isotherm extending approximately 2-m deep (Figure 4-9). Moreover, the isotherm plot indicates that the CCW discharge water undergoes cooling by the time it reaches the main lake to a point where temperatures are uniform across the downlake portion of the lake (Figure 4-9). Therefore, the combination of fast heat dissipation and homogenous water temperatures in upper water column indicate that higher water temperatures are mainly confined to the portion of the lake near the discharge canal confluence in the winter. 225 220 215 210 — E 205 — d- E 200 190 — 185 — 180 — 175 Sampling Locations 418 416 410 419.3 419.2 405 10 10 January 25, 2018 Average Station Discharge Temp: 18.23°C West Belews Creek Arm Belews Creek Arm -8 -6 -4 -2 0 2 4 6 8 10 12 14 16 Distance from Belews Dam (km) Figure 4-9. Near extreme winter scenario isotherm plot on Belews Lake. Plot generated from water temperature profile data measured on January 25, 2018. Temperatures in degrees Celsius. 40 38 36 34 32 30 28 26 24 22 20 18 16 14 12 10 8 6 4 2 0 Satellite imagery data, combined with water temperature profiles, shows that the BCSS thermal plume is not expected to block or inhibit access to any potential spawning habitat, spawning activities, or the development and growth of eggs, larvae, and early juveniles of RIS and the BIC. The extreme summer and winter scenarios indicated that the RIS would not be appreciably reduced due to normal operations. The potential for mortality associated with high discharge temperatures is negligible under the extreme conditions captured for BCSS and would be even less so under typical seasonal weather and lake -level conditions. 35 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.2 Limnology 4.2.1 Water Quality As stated in the temperature analysis section, Belews Lake exhibited a similar water temperature stratification and destratification regime in 2020 when compared to the 2017 monitoring period and historical data gathered by Duke Energy (Figure 4-10 and Duke Energy 2015). In addition to water temperature data, epilimnetic dissolved oxygen profiles in Belews Lake consistently exceeded the minimum NC water quality standard of 6 mg/L set for high quality waters (NCDWR 2019) in 2020 (Figure 4-10). Summer dissolved oxygen profiles from 2020 exhibited a metalimnetic depression in oxygen concentrations (Figure 4-10) coincident with the thermocline depth, as has been reported previously (Duke Energy 2015). This phenomenon, which is characteristic of southeastern reservoirs that undergo pronounced seasonal thermal stratification, is caused by the thermocline acting as a density barrier. This density gradient at the thermocline impedes vertical settling to the extent that overlying oxygen - consuming material settles to this depth and subsequently depletes the dissolved oxygen. During 2020, the metalimnetic dissolved oxygen depression was most clearly demonstrated at location 410.0 (Figure 4-10). Two-dimensional contour plots of epilimnetic dissolved oxygen profiles illustrate the seasonal distribution of dissolved oxygen throughout the reservoir in 2020. These contour plots indicate little spatial differentiation in dissolved oxygen concentrations in the winter months due to the relatively complete mixing of the water column (Figures 4-10). Summer dissolved oxygen profiles from 2020 depicted consistent prevalence of adequate dissolved oxygen concentrations across the reservoir in the epilimnion and within the discharge arm area (Figure 4-10). When comparing the summer dissolved oxygen profiles between locations, the data show that there is some variation in the degree of dissolved oxygen depletion in the deeper regions of the reservoir. In all, dissolved oxygen concentrations throughout Belews Lake provided sufficient habitat to support a BIC and allows for fish migration/movement throughout the surface waters (Figure 4-10 and Table 4-1). The temporal and spatial variability of pH in southeastern reservoirs is often correlated to lake productivity. During 2020, surface pH ranged from 6.9 to 8.5 with little difference among sampling locations across Belews Lake (Table 4-1). Higher values were observed at sampling sites located farther uplake where productivity was higher. Overall, pH values across the lake were within the range of NC water quality standards and would not adversely impact a BIC in Belews Lake. Specific conductance throughout Belews Lake was low, ranging from 45 to 82 µS/cm (Table 4-1). Low specific conductance indicated low ionic concentrations and little impact from wastewater sources in the reservoir. The uplake region of the reservoir (Zone F) exhibited lower specific conductance readings on average, which is characteristic of the headwaters of the lake. During 2020, specific conductivity measurements were within the historical ranges for all locations (Duke Energy 2015). 36 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Lc•7alora Localors #96.1 4103 41'92 408.1 410.5 419 2 408 I418 416 411 4 10 413 405 4}87] L 418 416 411 4 1}}15.3 01 5 5 10 • 15 • 20 • 25 • 30 35 #0 0 5 10 • 15 20 25 - 3] • 35 • #0 -6 4 -2 Aka Balaws Coa 4 0 2 4 6 8 10 2alaaa Cr.aic 1766TCC` 10 • 15 • 20 . 25 • 35 40 10 12 14 1i3 18 20 22 24 t 26 28 10 • 30 32 1� 34 20 - 36 25 - � 36 x • 35 • 40 5 - 4 -2 0 2 4 Wes[ ao'o,r Cmck Ciffrxo?IQnl 0 2 ii 3 4 5 ii 6 ii 7 8 9{ 0 Figure 4-10. Temperature (left panels) and dissolved oxygen (right panels) profile plots of Belews Lake during (top to bottom) February, April, July, and October 2020. Temperatures are in degrees Celsius, and dissolved oxygen is in mg/L. 37 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 4-1. Summary of surface (0.3 m) water quality measurements made in Belews Lake during 2020 monitoring period. Parameter NC water quality Zone A Zone B Zone C Zone D Zone E Zone F standard Temperature (°C) Dissolved Oxygen (mg/L) pH Specific conductivity (µS/cm) Mean Range Mean Range Mean Range Mean Range Mean Range Mean Range <_ 32 °C 23.1 11.0-37.6 21.8 10.8-34.5 22.0 10.9-35.7 21.2 11.0-33.2 21.1 11.0-32.4 19.71 9.4-30.8 >_ 6.0 mg/L 8.4 7.2-9.7 8.5 7.4-9.9 8.4 7.2-9.5 8.6 7.4-9.7 8.8 7.6-10.0 9.3 8.4-10.6 6.0-9.0 7.4 7.2-7.6 7.5 7.3-7.8 7.3 6.9-7.6 7.5 7.0-7.9 7.5 7.0-7.7 7.5 6.9-8.5 81 79-83 77 66-82 80 76-82 80 79-82 80 77-81 67 45-78 38 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.2.2 Water Chemistry During 2020, various trends in Belews Lake water chemistry were observed (Tables 4-2 and 4-3). Multiple state -certified laboratories were utilized for nutrients analysis. These laboratories had different reporting limits, constraining the ability to make comparisons between zones. Regardless, nutrient concentrations in the lake were low and trends could made when including historical data at the lower reporting limit (Appendix C). The uplake region closest to the headwaters of the lake (Zone F) exhibited the highest concentrations of nutrients in Belews Lake as evidence of total phosphorous, nitrate -nitrite, and total Kjeldahl nitrogen (TKN) values (Table 4-5). Nutrients measured in lower zones of the lake were typically much lower than that of the areas near the headwaters. The water chemistry in the upper end of the discharge arm (Zone B) was similar to that observed in the upper zone of the main lake (Zone F), which was most likely due to the similar water depth, clarity, and nutrient loading observed in this reach of the discharge arm (Table 4-2). Spatially, total phosphorous exhibited a decreasing trend from uplake to downlake (Table 4-2) which has been documented previously in Belews Lake as well as other reservoirs (Duke Energy 2015; Yurk & Ney 1989). Similar to phosphorous, orthophosphate was low throughout Belews Lake during the 2020 study period and historically. During 2020, only one orthophosphate sample was greater than the detection limit, which occurred in Zone F. Low concentrations of total phosphorous and orthophosphate indicated that Belews Lake was phosphorous -limited, as is common in freshwater lakes (USEPA 1978; Schindler et al. 2008). Despite being low, phosphorous concentrations in Belews Lake were sufficient to sustain primary productivity to support a BIC. Nitrogen concentrations (i.e., ammonia, nitrate -nitrite, and TKN) were low in all zones of Belews Lake during 2020 (Table 4-2), similar to ranges observed historically. TKN values were higher uplake than downlake which was likely due to the decomposition of organic materials such as sediment detritus, phytoplankton, or riparian inputs (e.g., leaf litter). Although overall concentrations of nitrogen were low, the ratio of nitrogen to phosphorous also supports that the lake was phosphorous -limited. These conditions support edible phytoplankton communities (rather than nitrogen -fixing blue-green algae) and an overall BIC. In Belews Lake, the major anions monitored were chloride and sulfate. During the 2020 monitoring period, both anions yielded consistent concentrations across zones and seasons (Table 4-2). Compared to historical data, both chloride and sulfate concentrations were below historical medians (Appendix C). All anion concentrations were within the range necessary for an overall BIC. The major cations monitored at Belews Lake were calcium and magnesium. These cations were only measured at locations 419.2 (Zone F) and 418.3 (Zone D), which provided an uplake and downlake comparison of constituents. During 2020, concentrations of both cations were consistent across the two locations and were similar to the historical ranges (Table 4-3). Hardness concentrations, which were calculated based on calcium and magnesium values, were within the historical ranges for both locations in 2020. The hardness concentrations observed in 2020 characterized the water in Belews Lake as soft (Table 4-3), falling below the NC water quality criteria (100 mg/L). All cation concentrations were within the range necessary to support an overall BIC. Turbidity values in Belews Lake generally decreased uplake to downlake, both historically and in 2020 (Table 4-2). Spatially, turbidity samples were highest in the two zones farthest uplake (Zones 6 and F) and were lowest in the zone nearest the dam (Zone E; Table 4-2). To add, mean turbidity values were 39 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station below the NC water quality criteria for lakes (25 NTU; Table 4-2) for all zones except Zone F which was closer to the headwaters of the lake. Similar to turbidity, secchi depth is a visual measure of water clarity by evaluating light penetration into the water column, therefore the greater the secchi depth, the greater the water clarity. Secchi depths were shallowest uplake and deepest downlake (Table 4-2). During 2020, mean secchi depths ranged from 0.1 m in Zone F to 3.1 m in Zone D. Based on these depths collected in 2020, secchi values were generally within the historical range and revealed that Belews Lake exhibits characteristics of an oligotrophic waterbody. Both turbidity and secchi depth were within the ranges that would support a BIC in Belews Lake. During 2020, all arsenic and selenium concentrations and all but one zinc concentration were below the lab reporting limits (Table 4-3). Data from the 2020 monitoring period also showed total copper concentrations that were within the historical ranges. To add, mean concentrations for total copper were below the NC water quality criteria (2.7 µg/L chronic and 3.6 µg/L acute). Similarly, low-level mercury concentrations were within the historical range. By the 1990s, the water chemistry in Belews Lake exhibited recovery from the legacy metals loading that led to the fish community collapse as noted in previous studies (Duke Energy 2015). As such, trace metals concentrations collected during 2020 were below the NC water quality standards and those collected since 2001 suggest these constituents should no longer be impacting the fish community or negatively affecting the overall BIC of Belews Lake (Table 4-3 and Appendix C). 40 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 4-2. Summary of nutrients, major anions, and physical parameters in Belews Lake during 2020. Non -detect values are presented to the reporting limit. NC Water Quality Criteria' Zone B Zone C Zone D Zone E Zone F Mean Range Mean Range Mean Range Mean Range Mean Range Nutrients' Total phosphorus N/A 0.05 <0.050-0.050 0.033 0.016-<0.050 0.029 0.006-<0.050 0.029 0.007-<0.050 0.083 <0.050-0.123 (mg/L) Orthophosphate N/A 0.028 <0.005-<0.050 0.028 <0.005-<0.050 0.028 <0.005-<0.050 0.028 <0.005-<0.050 0.028 <0.005-<0.050 (mg/L) Ammonia N/A <0.06 <0.02-<0.10 <0.06 <0.02-<0.10 <0.06 <0.02-<0.10 <0.06 <0.02-<0.10 <0.06 <0.02-<0.10 nitrogen (mg/L) Nitrite + nitrate WS: 0.01 0.148 0.095-0.200 0.140 0.077-0.195 0.132 0.079-0.183 0.127 0.071-0.178 0.148 <0.020-0.282 nitrogen (mg/L) mg/L Total Kjeldhal N/A 0.30 0.23-0.36 0.34 0.19-0.56 0.18 0.10-0.22 0.22 0.19-0.26 0.49 0.25-0.74 nitrogen (mg/L) Total organic N/A 3.4 3.2-3.5 3.0 2.9-3.0 2.9 2.7-3.1 3.0 2.7-3.3 4.5 3.6-5.6 carbon (mg/L) Major Anions Chloride (mg/L) 230 mg/L 5.1 4.8-5.3 5.5 5.2-5.7 5.6 5.2-5.9 5.6 5.2-5.9 3.7 2.7-4.9 Sulfate (mg/L) WS: 250 5.9 5.8-6.0 6.2 5.9-6.5 6.3 5.9-6.6 6.2 5.9-6.6 3.6 2.7-5.1 mg/L Physical Turbidity (NTU) 25 NTU 16.2 1.57-30.9 6.57 2.01-13.4 2.22 1.70-3.65 1.90 1.83-2.03 45.1 3.45-97.0 Secchi depth (m) N/A 1.7 0.4-3.0 1.3 0.8-2.5 2.5 2.1-3.1 2.3 2.0-2.6 0.8 0.1-1.7 Alkalinity (TIP) 20,000 21 19-22 22 22-22 22 21-23 22 21-23 19 14-24 mg/L3 Total Dissolved N/A 65 62-68 64 61-70 62 56-78 65 54-73 73 56-88 Solids NC Aquatic Life Criteria (ALC; 2017) water quality standard; water supply (WS) criteria were noted where ALC were not available. z RDL (result detection limit) and LOQ (limit of quantitation) varied for nutrient parameters collected during the winter and summer sampling period. See Appendix D for table listing the RDL and LOQ values for nutrients. Therefore, summary statistics reflect both RDL and LOQ values. All nutrient samples were analyzed with the sample laboratory methods. 3 EPA National Recommended Water Quality Criteria for Aquatic Life used when NC standard is not available 41 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 4-3. Summary of major cations, metals, and hardness (calculated) in Belews Lake during 2020. Non -detect values are presented to the reporting limit. Parameter NC Water Quality Criteria' Zone D Zone F Mean Range Mean Range Calcium (mg/L) Magnesium (mg/L) Arsenic, total (µg/L) Copper, total(µg/L) Mercury, low-level (ng/L) Selenium, total (µg/L) Zinc, total(µg/L) Total Hardness (mg/L) N/A N/A A: 340 µg/L / C: 150 µg/L A: 3.6 µg/L2/ C: 2.7 µg/L2 12.0 ng/L 5.0 µg/L A/C: 36 µg/L2 WS: 100 mg/L Major Cations 5.55 2.71 5.43-5.67 2.61-2.80 4.83 2.23 4.34-5.32 2.00-2.45 Metals <1 <1—<1 <1 <1—<1 1.09 <1.00-1.18 2.04 <1.00-3.07 0.528 <0.500-0.555 3.465 <0.500-6.430 <1 <1—<1 <1 <1—<1 <5.00 <5.00—<5.00 5.24 <5.00-5.48 25.0 24.3-25.7 21.3 19.1-23.4 NC Aquatic Life Criteria (ALC; 2017) water quality standard; water supply (WS) criteria were noted where ALC were not available. 'Acute (A) and chronic (C) ALC for these metals are hardness dependent and apply as a function of the parameter's water effect ratio which is set forth in 15A NCAC 02B .0211 (NCDEQ 2017). The values presented here are based on a hardness value of 25 mg/L. 42 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.2.3 Chlorophyll -a Chlorophyll -a concentrations were measured for each location from 2020 and compared spatially among zones. In general, chlorophyll -a concentrations in Belews Lake were low. Mean concentrations across zones ranged from <2.0 µg/L in zones D and E to 26.8 µg/L in Zone F (Table 4-4). Higher concentrations in Zone F were due to watershed inputs (e.g., higher nutrient concentrations) entering Belews Lake from upstream tributaries. Seasonally, concentrations were generally highest in the winter. However, this pattern was mostly only observed in Zone F, as most other samples were near or below the reporting limit (Table 4-4). Combining chlorophyll -a, secchi depth, and total nutrient data suggested that conditions in Belews Lake were reflective of an oligotrophic waterbody (Weiss and Kuenzler 1976; Carlson 1977; USGS 1982; NCDENR 2010). Chlorophyll -a samples collected during 2020 period had concentrations below the NC water quality criteria of 40 µg/L. As noted above, the higher concentrations observed at Zone F during the winter months were commensurate with the nutrients afforded near the headwaters of the lake. The data also indicated that that there was no trend in chlorophyll -a concentrations between thermally -influenced zones and areas uplake and downlake. Thus, this would suggest that there was no impact from operations of BCSS. Table 4-4. Chlorophyll -a concentrations (in µg/L) collected semiannually from five zones in Belews Lake during 2020. Zone B Zone C Zone D Zone E Zone F Winter Summer Mean 2.2 2.0 <2.0 <2.0 26.8 Range n/a <2.0-2.1 <2.0 <2.0 17.2-36.4 Mean <2.0 2.2 <2.0 <2.0 3.0 Range n/a <2.0-2.7 <2.0 <2.0 <2.0-4.0 4.3 Plankton Rationale In one of the most recent and independent reviews of CWA §316(a) language, Coutant (2013) states that there is "essentially no risk of aquatic damage from the thermal discharge" on a biological community considered LPI in the BIC framework, and therefore; "A low potential impact demonstration can be a "short form" demonstration with less extensive (and expensive) studies. In practice, LPI has generally been applied to particular biotic categories (such as phytoplankton) rather than for the whole site." Such a determination was made for Belews Lake plankton communities in the final Study Plan, using recommendations by Coutant (2013), the EPA §316(a) guidance manual (EPA 1977), and results of plankton data collected at another Duke Energy reservoir with a thermal discharge (Duke Energy 2019b). Although a plankton sampling program was initiated on Belews Lake in 1984, this effort collected plankton solely for selenium analyses, with no community information available. As such, no historical data are included in this assessment. Three decades worth of plankton data collected in Lake Norman suggested high inter -annual variability with little beneficial information gained on lake health relative to other, higher trophic level community data such as fish (Duke Energy 2019b). Therefore, the BIC determination for Belews Lake does not include an assessment of plankton communities, which are considered LPI. 43 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.4 Habitat Formers Surveys of aquatic plants in Belews Lake revealed limited growth of submersed and floating leaf aquatic plants, however several locations were noted to contain varying densities of emergent plants (Figure 4- 11). Sterile Grass Carp Ctenopharyngodon idella have been historically stocked to control the invasive aquatic plant, Hydrilla Hydrilla verticillata. Grass carp feed almost exclusively on submersed vegetation, and thus sustained stockings can lead to declines in both Hydrilla and other submersed aquatic plant species as well. Grass Carp were stocked in Belews Lake (2005-2018) to control Hydrilla populations. The lack of submersed and floating leaf aquatic plants can most likely be explained by continued pressure from an existing Grass Carp population. As these fish are sterile, the Grass Carp population should continue to decline over time. The most dominant and widely distributed species in Belews Lake was Water Willow Justicia americana, which was found at various densities in every zone of the main lake (i.e., Zones C, D, E, and F) (Figure 4-12). Arrowhead Sagitatia Iancifolia, Arrow Arum Peltandra virginica, Button Bush Cephalanthus occidentalis, Bulrush Scirpus sp., and Umbrella Sedge Cyperus sp. were also noted throughout. Isolated populations of Swamp Rosemallow Hibiscus moscheutos, Cattail Typha sp., Giant Cutgrass Zizaniopsis millacea, and Giant Reed Arundo donax were also identified during the survey. Several locations contained more than 3 species; thus, such points were designated as diverse wetland communities. A benthic scum was noted throughout zones A and B, however an identification could not be made. This scum was most likely a consortium of decaying filamentous algal cells and other bacteria which are common in waterbodies during the fall. The number of vegetated points and associated density varied by zone. Zone B was the most vegetated zone sampled, with approximately 23.3% of points vegetated (24 of 103 points sampled). Zones F and A followed with 20.89% and 12.67% respectively (61 of 292 and 19 of 150 points sampled). Zones C, E, and D were the least vegetated at 7.8%, 4.8%, and 2.6% of points vegetated (Figure 4-13). Zones B and F also shared the greatest number of high density (rating = 4) points (Figures 4-13). Distribution and density of vegetated points was to be expected, as the reservoir morphology in highly vegetated zones was also conducive to plant growth. For example, highly vegetated zones B and F were located in the more riverine portions of the reservoir, characterized by shallow water and an expansive littoral zone for aquatic plant growth (Figures 4-14 and 4-15). On the contrary, poorly vegetated zones C, D and E, were located in the more lacustrine, deep water portions of the reservoir with less shallow water suitable for aquatic plant growth (Figures 4-14 and 4-15). There was no apparent trend in locations and density of vegetated points and zones of thermal influence. 44 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Figure 4-11. Aquatic vegetation population densities and distribution noted at survey locations. 45 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Figure 4-12. Distribution of Water Willow Justicia americana at sampled sites by zone. 46 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Density • 1 Figure 4-13. Aquatic vegetation population densities and distribution at survey locations by thermal influence zone. 47 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station F E 3 Percentage of Points Vegetated per Zone by Density Class ■ C '; Ei% 10% 15% 2C5; 23 . 111. 2 NI3 ■4 Figure 4-14. Percentage of points surveyed found to be vegetated per zone of thermal influence by density class (1-4). 48 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 70 60 50 40 30 20 10 0 N. x Species Summary of Vegetated Points by Zone 3 1 ■ > 3 species ■ J. americana ■ Typha sp. ■ Cyperus sp. ■ H. moschuetos ■ Scirpus sp. ■ C. occidentalis ■ S. lancifolia ■ P. virginica Figure 4-15. Species summary of vegetated points by zone of thermal influence. 4.5 Macroinvertebrate Rationale An LPI determination was made for Belews Lake benthic macroinvertebrates (insects) due to "essentially no risk of aquatic damage from the thermal discharge," as defined by Coutant (2013). Similar to plankton, a benthic macroinvertebrate monitoring program was implemented on Belews Lake in 1984 to monitor and quantify bioaccumulation of selenium in select benthic macroinvertebrates. However, unlike the plankton monitoring program, an additional aspect of macroinvertebrate sampling was implemented in 1991 to examine the density and diversity of these populations. In each year, five replicate bottom samples were collected each from an uplake, a midlake (near the BCSS condenser cooling water discharge canal), and a downlake location. Results from these surveys show that the density and diversity of benthic macroinvertebrates was highly variable, displaying no temporal or spatial trend; therefore, similar to plankton, little beneficial information can be gained on lake health relative to other, higher trophic level community data such as fish (Duke Energy 2015). 49 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.5.1 Mussel Community A total of two species of freshwater mussels were documented during the 2020 surveys. Eastern Elliptio Elliptio complanata was abundant in five of the zones and represented 99.6% of the 4,036 mussels collected. The Paper Pondshell Utterbackia imbecillis (not native to this river basin) was rarely encountered in these surveys (14 individuals) and was collected in Zones B, E, and F. There is significant taxonomic uncertainty in the genus Elliptio, and we observed morphological variability within each of the zones. Genetic studies are ongoing at several universities, and preliminary results suggest that there will be numerous revisions within this species complex. Regardless, no mussel species currently listed as threatened or endangered by NCWRC or USFWS were documented in Belews Lake. Zone D had a very high CPUE of 193.2 mussels/hr and CPUE was also high in the remaining zones except for Zone A (Figure 4-16). No mussels were collected in Zone A, however, thermophilic algae covered the substrate which limited the available habitat in this zone. The mean length of Eastern Elliptio (Figure 4-17) was smallest in Zone C (59.0 mm) and largest in Zone F (70 mm) which may be associated with the productivity gradient from uplake areas to downlake. Mostly adult mussels were observed during these surveys, but several young individuals were encountered in Zone C, D and E, suggesting that recruitment has occurred. Freshwater mussels are an important component of a balanced aquatic community and the abundance of Eastern Elliptio suggests that appropriate habitat conditions exist in Belews Lake. 50 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station CPUE (number/hr) 225 - 200 - 175 - 150 - 125 - 100 - 75 - 50 - 25 - 0 A B C D E F Figure 4-16. Mean catch rate (CPUE) by number of all freshwater mussels collected in 2020 within six zones in Belews Lake. Mussel Length (mm) 80 - 70 - 60 - 50 - 40 A B C D E F Figure 4-17. Mean length of Eastern Elliptio collected in 2020 within six zones in Belews Lake. Error bars are 90% confidence intervals. 51 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.6 Fish Community 4.6.1 Fish Community Assessment The spring 2020 electrofishing surveys were conducted at mean water temperatures ranging from 16.6 °C (Zone C) to 18.2 °C (Zones B and E; Table 4-5). Dissolved oxygen, conductivity, and pH were similar among zones and were within ranges that would support fish assemblages typical of a piedmont reservoir (Table 4-5). The 2020 spring electrofishing surveys resulted in the collection of 2,208 individuals (16 individual species and a centrarchid hybrid complex; Table 4-6) with a total weight of 375.3 kg (Table 4-9). Bluegill were the most abundant species, and Alabama Bass had the highest total biomass of any species. Table 4-5. Mean (and range) of water quality parameters for each zone in Belews Lake during spring 2020 electrofishing. Parameter A B C D E F Temperature (°C) 17.6 (16.8- 18.2 (18.1- 16.6 (16.6- 18.1 (17.5- 18.2 (17.8- 17.6 (17.5- 18.7) 18.5) 16.7) 18.5) 18.5) 17.7) Dissolved Oxygen 9.1 (9.1-9.2) 9.8 (8.7-10.9) 8.9 (8.4-9.2) 9.5 (9.2-9.8) 8.9 (8.2-9.6) 9.0 (8.8-9.2) (mg/L) Conductivity (µS/cm) 81 (79-83) 83 (82-83) 81 (78-83) 80 (77-83) 82 (79-83) 68 (66-70) pH 7.7 (7.5-7.8) 7.8 (7.4-8.2) 7.8 (7.6-7.9) 7.7 (7.5-7.8) 7.7 (7.4-7.9) 7.7 (7.6-7.8) Table 4-6. Number of fish collected from electrofishing within six zones of Belews Lake during spring 2020. Species A B C D E F Origin No. No. No. Kg No. Kg No. Kg No. Kg No. Kg Centrarchidae Alabama Bass Introduced 56 61 61 20.38 60 20.38 79 25.18 49 13.00 15 5.14 Black Crappie Native 9 18.67 Bluegill Native 483 368 368 2.25 97 2.25 44 0.64 83 1.32 297 4.76 Green Sunfish Introduced 2 15 15 0.09 2 0.09 5 0.17 4 0.06 Hybrid sunfish Hybrid 4 9 9 0.83 15 0.83 5 0.36 8 0.94 2 0.28 Largemouth Bass Native 10 22 22 10.82 12 10.82 8 6.37 20 16.09 46 20.91 Redbreast Sunfish Native 1 1.03 25 1.03 22 0.96 37 1.49 16 0.65 Redear Sunfish Introduced 27 54 54 2.38 43 2.38 16 1.73 13 1.49 7 1.17 Warmouth Native 6 6 1 0.02 White Crappie Introduced 9 2.66 Clupeidae Gizzard Shad Native 4 4 13 4.56 Cyprinidae Common Carp Introduced 1 1 1 3 15.88 Grass Carp Introduced 1 Satinfin Shiner Native 1 0.01 Ictaluridae Channel Catfish Introduced 1 1 2 2.31 9 6.10 Moronidae White Perch Introduced 1 1 1 0.19 Percidae Yellow Perch Native 3 0.07 Total 585 542 542 37.78 254 37.78 174 35.24 217 36.81 436 81.13 Number of taxa 11 9 9 7 6 8 16 52 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station The mean CPUE of fish by number (p=0.001) varied among zones during spring 2020 (Figure 4-18). The mean catch rate was significantly higher in Zone A than in zones C, D, and E and significantly higher in Zone B than zones D and E. The mean CPUE of fish by weight was not different (p=0.195) among zones during spring 2020 (Figure 4-18). The patterns generally showed higher catch rates in zones with higher nutrient levels (see Section 4-2). Additionally, zones with higher thermal influences (A and C) did not have catch rates outside what would be expected for the nutrients in those zones. These patterns match what has been observed in Belews Lake since 1994 (Duke Energy 2015). CPUE (number/hr) 1000 - 800 - 600 - 400 - 200 - z yz xy x x xyz 100 - 80 - 2E 60 - 0) W U 40- 20 - A B C D E F A B C D E F Zone Figure 4-18. Mean catch rate (CPUE) by number (top panels) and by weight (bottom panels) of all species collected within six zones from electrofishing in Belews Lake during spring 2020. Error bars are 90% confidence intervals. Letters over bars indicate significance at a = 0.10. 53 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station The percentages of fish species with different pollution tolerance classifications, trophic levels, and the percent of sunfish identified as hybrids differed among zones of Belews Lake during 2020 (Table 4-7). The differences between zones of tolerance rating and trophic status were primarily related to variable catches of Bluegill, which was lower in zones E, D, and C. No zones were dominated by species that were classified as "tolerant", and multiple trophic levels were present in all zones. Overall, there were no patterns in proportions of tolerance rating, trophic levels, or percent hybrid sunfish that would indicate negative impacts from the operations of BCSS. Table 4-7. Percent pollution tolerance, trophic guild, and percent of hybrids for fish collected from electrofishing within six zones of Belews Lake during spring 2020. Category A BCDE F Tolerance rating Tolerant 0.7 3.0 10.6 12.6 19.4 5.5 Intermediate 88.9 84.1 59.8 39.1 54.4 90.6 Not defined 10.4 12.9 29.5 48.3 26.3 3.9 Trophic levels Piscivore 11.3 15.5 28.3 50.0 31.8 19.0 Insectivore 88.4 83.4 71.7 50.0 67.3 75.2 Omnivore 0.2 1.1 0.0 0.0 0.9 5.7 Herbivore 0.2 0.0 0.0 0.0 0.0 0.0 Percent hybrids Sunfish 0.8 2.0 8.2 5.7 5.5 0.6 The fall 2020 gill netting survey was conducted at mean water temperatures ranging from 21.1 °C (Zone F) to 26.8 °C (Zone A; Table 4-8). Dissolved oxygen, conductivity, and pH were similar among zones and were within ranges that would support fish assemblages typical of a piedmont reservoir (Table 4-8). The survey resulted in the collection of 393 individuals (13 species from five families; Table 4-9) with a total weight of 127.4 kg. Alabama Bass were the most abundant species numerically, and Gizzard Shad had the highest total biomass of any species (Table 4-9). Table 4-8. Mean (and range) of water quality parameters for each zone in Belews Lake during fall 2020 gill netting. Parameter Temperature (°C) Dissolved Oxygen (mg/L) Conductivity (µS/cm) pH A B C D E F 26.8 (26.4- 24.7 (24.4- 26.0 (25.6- 23.9 (23.2- 24.0 (23.7- 21.1 (20.7- 27.1) 25.3) 26.4) 24.3) 24.2) 21.7) 7.2 (6.7-7.7) 7.8 (7.7-7.9) 7.5 (7.3-7.6) 7.8 (7.6-8.0) 7.8 (7.6-8.1) 8.1 (7.7-8.3) 80 (76-82) 81 (80-82) 81 (81-82) 80 (80-81) 80 (79-81) 78 (77-78) 7.2 (7.1-7.3) 7.5 (7.3-7.5) 7.4 (7.3-7.4) 7.3 (7.3-7.4) 7.3 (7.3-7.3) 7.1 (7.0-7.1) 54 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 4-9. Number of fish collected from gill netting within six zones of Belews Lake during fall 2020. Species A B C D E F Origin No. Kg No. Kg No. Kg No. Kg No. Kg No. Kg Centrarchidae Alabama Bass Introduced 23 5.58 21 3.80 26 6.93 25 7.53 21 6.88 16 3.36 Black Crappie Native 2 0.78 5 1.67 4 0.93 1 0.54 7 1.30 Bluegill Native 2 0.12 3 0.02 1 0.01 Green Sunfish Introduced 1 0.01 1 0.01 Largemouth Bass Native 3 1.65 1 1.05 6 5.59 7 2.79 Redear Sunfish Introduced 4 0.48 5 0.53 4 0.68 1 0.15 1 0.18 Warmouth Native 4 0.28 Clupeidae Gizzard Shad Native 16 4.72 28 9.63 40 14.77 14 6.31 10 4.48 10 2.87 Threadfin Shad Introduced 2 0.02 Cyprinidae Common Carp Introduced 1 1.69 Ictaluridae Channel Catfish Introduced 22 6.23 14 5.53 4 2.47 4 2.32 5 3.74 11 4.27 Flathead Catfish Introduced 1 0.91 2 2.75 Moronidae White Perch Introduced 4 0.38 3 0.41 1 0.10 6 0.96 Total 72 18.17 88 26.22 85 29.70 45 16.32 43 21.22 60 15.75 Number of taxa 11 9 9 5 5 8 The mean CPUE of fish by number during fall gill net surveys ranged from 10.8 per net -night in Zone E to 22.0 per net -night in Zone B (Figure 4-19). These catch rates and the CPUE by weight did not vary among zones during fall 2020 (p>0.10; Figure 4-19). 55 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station CPUE (fish/net-night) CPUE (kg/net-night) 40 - 30 - 20 - 10 - 0 i 12 - 10 - A B C D E F A B C D E F Zone Figure 4-19. Mean catch rate (CPUE) by number (top panels) and by weight (bottom panels) of all species collected within six zones from gill netting in Belews Lake during fall 2020. Error bars are 90% confidence intervals. 56 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.6.2 RIS Population Assessment All five species of centrarchid RIS (Alabama Bass, Largemouth Bass, Bluegill, Redbreast Sunfish, Redear Sunfish) were collected in all zones of Belews Lake during spring 2020 electrofishing except Redbreast Sunfish in Zone B. The CPUE for these species were compared using only stock size and greater individuals because this is the minimum size at which they are effectively captured with traditional sampling gears (i.e., electrofishing; Neumann et al. 2012). The mean CPUE of the centrarchid RIS varied among zones (Figure 4-20). Bluegill had mean CPUE highest in Zone B, Alabama Bass CPUE was highest in Zones A and D, Redbreast Sunfish CPUE was highest in Zone E, and Redear Sunfish CPUE was highest in Zone C (Figure 4-20). The differences in mean CPUE among zones for RIS was primarily related to nutrient concentrations and habitat preferences, and none of these differences of mean RIS CPUE among zones appeared to be related to the thermal influences of BCSS. The mean CPUE of Largemouth Bass and Alabama Bass among zones were negatively correlated. Although not significantly different among zones, Largemouth Bass CPUE was lower in the deeper, clearer portions of the reservoir where they have likely been displaced by Alabama Bass through congeneric competition (Sammons and Bettoli 1999; Long and Fisher 2000; Pope et al. 2005). Similar trends have been documented in other lakes where Alabama Bass have been introduced (Duke Energy 2019b). Additionally, Redbreast Sunfish relative abundance was lower in the upper portions of the reservoir, which is a pattern documented in other Piedmont reservoirs (Duke Energy 2019b). Overall, the lake -wide mean CPUE for Largemouth Bass was below the 25th percentile for the eco- region (Brouder et al. 2009), but if Alabama Bass were included, this mean lake -wide CPUE would be at the 75th percentile. Conversely, the lake -wide mean CPUE for Bluegill was at the 50th percentile for the eco-region (Brouder et al. 2009). 57 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station CPUE (number/hr) CPUE (number/hr) 100 - 80 - 60 - 40 - 20 - 60 50 40 30 20 10 Alabama Bass z yz yz z yz y I A BCD E F Largemouth Bass z z z z z z Zone E CPUE (number/hr) CPUE (number/hr) CPUE (number/hr) 350 300 250 200 150 100 50 0 60 - 50 - 40 - 30 - 20 - 10- 0 80 60 40 20 0 y A B y y yz yz Bluegill Y Y Yz 111 D E F Redbreast Sunfish zy z yz A B D E F Redear Sunfish yz yz z yz y Y Zone D E 11 F Figure 4-20. Mean catch rate (CPUE) by number of stock size and larger centrarchid RIS collected within six zones from electrofishing in Belews Lake during spring 2020. Error bars are 90% confidence intervals. Letters over bars indicate significance at a = 0.10. Length frequency analyses indicated multiple age -classes of centrarchid RIS throughout Belews Lake during spring 2020 electrofishing. Each RIS had size structures indicative of multiple age classes in each zone with a few exceptions where sample sizes were small. Overall, the size structures of all centrarchid RIS within each zone were similar, suggesting no impact from the operation of BCSS. The size structures for Largemouth Bass and Alabama Bass were skewed toward larger individuals when compared to the average for the eco-region (Brouder et al. 2009). Fish from the youngest available age class (i.e., age-1 during spring electrofishing) were captured for both species, an indication of spawning and recruitment to age-1, but a disproportionately low number of bass were captured under 300 mm (Figure 4-21). Individuals of this intermediate size are those that would grow and recruit to larger sizes in future years. The skewed size structures of Largemouth Bass and Alabama 58 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Bass in Belews Lake may have indicated low mortality or low prey abundance limiting growth of larger individuals, but overall these populations were sustainable. The size structure for Bluegill was skewed toward smaller individuals, whereas Redbreast Sunfish and Redear Sunfish had size structures similar to the average for the eco-region (Brouder et al. 2009; Figure 4-22). The skewed size structure of Bluegill towards smaller individuals may have been indicative of high density, but high mortality (e.g., predation) was a more likely cause. The CPUE of Bluegill in Belews Lake at the 50th percentile for the eco-region, suggesting density -dependent growth was unlikely, whereas the black bass CPUE was at the 75th percentile. The combination of predator size structure (Largemouth Bass and Alabama Bass) and prey size structure (Bluegill and Redbreast Sunfish) suggested these populations may have naturally reached a "big bass" equilibrium (Willis et al. 1993). Overall, the patterns documented in length -frequency and CPUE among zones for all five centrarchid RIS indicated the presence of multiple age classes including both age-1 fish and the largest mature individuals. No differences were observed that would suggest negative impacts from the operations of BCSS. 59 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 30 25 20 15 10 5 0 30 25 A 20 - 15 - 10 - 5- 0 30 25 - 20 - 15- 10- 5- 0 60 50 B D 30 25 20 - 15- 10- 5- 0 30 25 20 15 10 A 0 30 25 - 20 15 10 5 0 30 25 20 n 20 15 15 10 1 n 10 5i IIIIIII 5 0 30 25 - 20 - 15- 10- 5- 0 30 25 - 20 - 15 - 10 - 5- 0 ri �n 100 200 300 400 Total length (mm) E F �n 500 0 30 25 20 15 10 5 0 30 25 20 15 10 5 0 B P C n D n E i F 100 200 300 400 500 Total length (mm) Figure 4-21. Length -frequency of Largemouth Bass (left panels) and Alabama Bass (right panels) collected within six zones from electrofishing in Belews Lake during spring 2020. 60 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 60 50 40 30 20 10 0 60 50 40 30 20 10 0 A 100 80 - 60 - 40 - 20 - 0 20 15 10 5 0 60 20 c 50 40 30 20 10 0 60 50 8 40 m 0 D A B 15- 10- 0 20 15 30 10 20 10 0 60 50 40 30 20 10 0 60 F 50 5 0 20 c D F 15- 10- 5- 0 20 r 15- 40 30 10 - 20 10 0 40 80 120 160 200 5- 0 F 50 40 30 20 10 0 50 40 30 20 10 0 50 40 30 20 10 0 50 40 30 20 10 0 50 40 30 20 10 0 50 40 30 20 10 A B c D E 0 F 80 120 160 200 80 120 160 200 240 280 Total length (mm) Figure 4-22. Length -frequency of Bluegill (left panels), Redbreast Sunfish (center panels), and Redear Sunfish (right panels) collected within six zones from electrofishing in Belews Lake during spring 2020. 61 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station The two species of RIS targeted with gill nets (Channel Catfish and Gizzard Shad) were collected in all zones of Belews Lake during fall 2020. The CPUE for these species were compared using only stock size and greater individuals because this is the minimum size at which they are effectively captured with traditional sampling gears (i.e., gill nets; Neumann et al. 2012). The CPUE of Channel Catfish did not differ among zones (p>0.10), whereas the CPUE of Gizzard Shad was significantly different among zones (Figure 4-23). The mean CPUE of this species in Zone C (10.0/net-night) was significantly higher than the mean CPUE in zones E (2.5/net-night) and F (3.3/net-night). However, this pattern did not appear to be related to the thermal influences of BCSS. 62 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station CPUE (fish/net-night) CPUE (fish/net-night) 8- 6 4- 2- 0 Channel Catfish z z z z z z 18- 16 - 14 - 12 - 10- A B C yz yz z yz Gizzard Shad Y Y A B C D Zone Figure 4-23. Mean catch rate (CPUE) of Channel Catfish and Gizzard Shad collected within six zones from gill netting in Belews Lake during fall 2020. Error bars are 90% confidence intervals. Letters over bars indicate significance at a = 0.10. During fall gill netting, a representative range of sizes of Channel Catfish and Gizzard Shad were captured (Figure 4-24). Sample sizes within each zone were limited, however multiple age classes were represented for each species in each zone. Importantly, no zone was limited to only small and young individuals or large and old individuals. These data suggest that dynamic population functions (i.e., 63 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station recruitment and growth) occurred in all parts of Belews Lake, and these populations had the ability to sustain themselves across seasons and years. 60 50 40 30 20 10 0 60 50 40 30 20 10 0 60 50 40 30 20 10 0 60 50 40 30 20 10 0 60 50 40 30 20 10 0 A 60 40 20 - B 60 40 20 0 D 60 40 20 200 250 300 350 400 450 500 550 Total length (mm) 0 60 40 20 A B c D 200 250 300 350 Total length (mm) 400 E Figure 4-24. Length -frequency of Channel Catfish (left panels) and Gizzard Shad (right panels) collected within six zones from gill netting in Belews Lake during fall 2020. The standard weight for a species (used in calculating relative weight, Wr) is calculated based off the 750h percentile weight. Therefore, fish with a Wr of 100 would have above average condition (Wege 64 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station and Anderson 1978). A Wr around 90 would be considered "average" condition, and Wr values below 80 would be "poor" (Murphy et al. 1990). Only data collected during the fall are included in this analysis because for spring -spawning fish (e.g., centrarchids, ictalurids), Wr can be artificially high or low depending on the spawning status of individual fish. Fish collected with both electrofishing and gill netting were included in the analysis. The condition for Largemouth Bass and Alabama Bass in Belews Lake during 2020 were below average with a mean Wr of 85 and 87, respectively. The mean Wr within a zone was variable for Largemouth Bass, ranging from 83 in Zone E to 97 in Zone A, whereas the mean Wr within a zone was consistent for Alabama Bass (Figure 4-25). Lake -wide mean Wr decreased with increasing size for both species (Figure 4-25). This decreasing trend could be indicative of limited prey availability for the largest individuals. Overall, no trends were apparent in Wr values for either species of black bass that could be attributed to operations at BCSS. Relative weight Relative weight 120 - 110 - 100 - 90 - 80 - 70 - 60 120 - 110 - 100 - 90 - 80 - 70 60 A B C D E F Zone T 1 No Data Relative weight Relative weight 120 110 100 90 80 70 60 120 110 100 90 80 70 60 T 1 T 1_ A B C D E F Zone 5 `-1 oo'1' o `,' ^ ``N oo y y p �J,e y`o ova e``e<< oao g goo 6ea e�e oee Length group Length group Figure 4-25. Largemouth Bass (left panels) and Alabama Bass (right panels) condition (relative weight) by zone of Belews Lake and by length category for fish collected during fall 2020. The horizontal line represents the median for each zone, the boxes represent the 25th and 75th percentile, and the whiskers show 10th and 90th percentiles. The mean Wr of Bluegill and Redear Sunfish in Belews Lake during 2020 was poor with mean values of 81 and 80, respectively. For both species, mean Wr in all zones and length groups were similar with mean Wr values ranging from 77 to 84 (Figure 4-26). The mean Wr of Redbreast Sunfish in Belews Lake was 91, and mean Wr values in the zones and length groups were also similar (Figure 4-26). It is possible the relative weights calculated for Redbreast Sunfish did not accurately assess condition. The standard weight equation for this species was created from historical data collected by Duke Energy and may not contain enough populations to accurately determine parameter values. Regardless, this metric does allow a comparison of overall condition between zones of the lake and among length 65 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station groups. The low relative weights for these sunfish species, especially Bluegill and Redear Sunfish, may be related to food limitations in the low productivity lake. However, no differences in condition were documented for any sunfish species that would indicate impacts from operations at BCSS. Relative weight Relative weight 120 - 110 - 100 - 90 - 80 - 70 - 60 T T 90 - 80 - 70 - 60 A B C D E F T 1_ T 1_ Zone No Data A-e ,h° do ytid QJagA e,a'� Q Length group Relative weight Relative weight 90 - 80 - 70 - 60 Relative weight Relative weight 120 - 110 - 100 - 90 - 80 - 70 - 60 No Data T T 90 - 80 - 70 - 60 A B C D E F 1 T _L Zone No Data '9\1'' a Length group 90 - 80 - 70 - 60 T C D Zone T T sae 5- oaa ka aova Length group Figure 4-26. Bluegill (top left panels), Redbreast Sunfish (top right panels), and Redear Sunfish (bottom panels) condition (relative weight) by zone of Belews Lake and by length category for fish collected during fall 2020. The horizontal line represents the median for each zone, the boxes represent the 25' and 75th percentile, and the whiskers show 10th and 90' percentiles. 66 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Channel Catfish in Belews Lake had below average relative weights with a mean of 86. However, Wr values were variable among zones from 81 in Zone A to 94 in Zone D (Figure 4-27). Gizzard Shad mean W, was average at 89, and this species also exhibited some variability among zones. Mean Wr within each zone was considered average (87-91) for all zones except Zone F where mean W, was 79 (Figure 4-27). Neither species showed trends in mean Wr across different length groups. Like the centrarchid RIS above, the patterns in condition for these two species did not indicate adverse impacts from operations at BCSS. Relative weight Relative weight 120 - 110 - 100 - 90 - 80 - 70 - 60 T 1 T 120 - 110 - 100 - 90 - 80 - 70 - 60 A 1_ C D E F Zone T 1 No Data No Data �p Tc�\ 0 OJQ'���``� ec.6 eV Length group Relative weight Relative weight 120 110 100 90 80 70 60 120 110 100 90 80 70 60 T 1 T 1 T A 8 C D Zone T 1 E F T L 1�0 Length group Figure 4-27. Channel Catfish (left panels) and Gizzard Shad (right panels) condition (relative weight) by zone of Belews Lake and by length category for fish collected during fall 2020. The horizontal line represents the median for each zone, the boxes represent the 25th and 75th percentile, and the whiskers show 10th and 90th percentiles. 4.6.3 Fish Community Similarities The ANOSIM determined the overall sample statistic from the Bray -Curtis non -metric, multidimensional scaling or similarity matrix was significant for both electrofishing (R = 0.573) and gill netting (R = 0.194), meaning dissimilarities existed among zones for both gear types. For electrofishing data, dissimilarities existed between both Zone B and Zone F and all other zones (Figure 4-28). These two zones were shallower and had the higher nutrient levels than other zones of the lake, which likely influenced the dissimilarities in the fish community. For the gill net data, strong dissimilarities only existed between zones E and A and zones E and C (Figure 4-29). These dissimilarities were related to differences in the catch of several species between the zones, however the overlap of zone clusters demonstrated these groupings did not suggest an overall pattern in the lake. No other meaningful dissimilarities were found. Critically, the clusters defined from both the spring electrofishing survey and the fall gill net survey did not indicate grouping based on thermal impact. 67 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Non -metric MDS Transform: Square root Resemblance: S17 Bray -Curtis similarity Similarity 60 70 80 Report Area A A D v B *C m E + F Figure 4-28. Bray -Curtis similarities of fish CPUE from spring electrofishing in Belews Lake during spring 2020. Non -metric MDS Transform: Square root Resemblance: S17 Bray -Curtis similarity Similarity 60 70 80 Report Area A A V B C • D F E E Figure 4-29. Bray -Curtis similarities of fish CPUE from fall gill netting in Belews Lake during fall 2020. 68 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.6.4 Seasonal RIS Distributions Thermal impacts from BCSS were more pronounced during winter and summer fish sampling. Mean water temperatures ranged from 12.0° C in Zone D to 17.4° C in Zone A in winter and from 31.5° C in Zone F to 35.7°C in Zone A in summer (Table 4-10). At least one individual of each species of RIS was captured in every zone each season, except Redbreast Sunfish (Tables 4-11 and 4-12). However, only 16 individuals of this species were captured in the discharge arm of Belews Lake (zones A and B) during the entire year. Some patterns were apparent in the seasonal data that suggest these species make horizontal movements (e.g., highest Gizzard Shad catch was in different zones in different seasons) and vertical movements (e.g., electrofishing catch decreased and gill net catch increased in summer for some species) throughout the year. Overall, these seasonal abundances suggested that while fish in Belews Lake do make movements throughout the year for optimal habitat and forage, the thermal discharge of BCSS does not create unsuitable habitat within any zones of the lake for any species. A full depiction of the number and biomass of species captured seasonally with both gear types can be found in Appendix E. Table 4-10. Mean (and range) of water temperature (°C) for each zone in Belews Lake during winter and summer 2020 electrofishing and gill netting. Parameter A B C D E F 17.4 16.1 15.1 12.0 13.1 12.5 Winter (16.9-17.8) (15.9-16.2) (14.5-15.6) (11.8-12.1) (12.9-13.3) (11.6-13.7) Summer 35.7 34.3 34.2 33.1 32.9 31.5 (34.7-36.6) (33.5-35.4) (33.5-34.9) (32.1-33.4) (32.2-33.8) (30.8-32.1) 69 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 4-11. Numbers of RIS collected from electrofishing within six zones of Belews Lake during quarterly sampling in 2020. A B C D E F W Sp Su F W Sp Su F W Sp Su F W Sp Su F W Sp Su F W Sp Su F Alabama Bass 29 56 2 25 49 61 22 45 26 60 27 77 22 79 36 52 36 49 16 49 16 15 42 46 Bluegill 546 483 340 408 564 368 348 506 28 97 149 146 186 44 302 176 122 83 118 117 187 297 434 229 Largemouth 16 10 4 6 11 22 69 16 1 12 0 6 2 8 42 2 6 20 4 9 18 46 57 25 Bass Redbreast 0 1 1 2 2 0 5 5 4 25 41 37 6 22 132 61 4 37 26 29 2 16 19 21 Sunfish RedearSunfish 36 27 1 11 58 54 10 29 19 43 10 13 3 16 9 7 4 13 4 2 36 7 14 18 Gizzard Shad 0 0 0 0 12 4 0 3 0 0 0 1 0 0 1 2 0 0 0 0 10 13 6 2 Channel 0 0 0 2 0 1 0 1 0 0 1 0 0 0 2 1 1 2 3 0 0 9 8 9 Catfish Table 4-12. Numbers of RIS collected from gill netting within six zones of Belews Lake during quarterly sampling in 2020. A B C D E F W Sp Su F W Sp Su F W Sp Su F W Sp Su F W Sp Su F W Sp Su F Alabama Bass 23 4 4 23 30 9 25 21 31 16 5 26 14 9 17 25 31 11 3 21 11 8 5 16 Bluegill 1 0 1 0 10 0 0 2 1 0 0 3 0 2 0 1 1 0 0 0 0 1 0 0 Largemouth Bass 6 0 1 0 7 2 0 3 1 1 1 1 3 1 1 0 4 4 1 6 3 5 0 7 Redbreast Sunfish 0 0 0 0 0 0 0 0 2 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 Redear Sunfish 0 0 0 4 3 2 9 5 2 1 5 4 1 1 6 1 2 2 6 0 0 1 1 1 Gizzard Shad 12 4 1 16 32 38 32 28 13 18 6 40 9 5 14 14 2 8 2 10 10 75 18 10 Channel Catfish 10 9 19 22 7 17 41 14 3 4 3 4 2 3 1 4 0 1 8 5 9 12 18 11 70 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.6.4.1 Mayo Reservoir Comparison Electrofishing and gill netting on Mayo Reservoir was conducted quarterly at water temperatures representative of those seasons in a North Carolina reservoir without thermal effluent (Table 4-13). Table 4-13. Mean (and range) of water temperature for each zone in Mayo Reservoir during quarterly 2020 electrofishing and gill netting. Parameter B E G Winter 8.3 (7.8-8.8) 8.4 (8.2-8.5) 9.2 (9.1-9.2) Spring 16.3 (15.9-16.8) 16.8 (16.4-17.3) 18.0 (16.6-19.2) Summer 32.3 (31.7-33.2) 31.4 (30.7-32.3) 31.7 (30.5-33.2) Fall 19.7 (19.4-19.8) 20.5 (20.5-20.5) 21.2 (19.9-22.1) The percentages of fish species with different pollution tolerance classifications, trophic levels, and the percent of sunfish identified as hybrids were generally similar among zones of Mayo Reservoir during spring 2020 (Table 4-14). Similar to Belews Lake, the percent of fish classified as "tolerant" was higher in down -lake areas. Additionally, fish classified as omnivores (primarily Gizzard Shad) were captured almost exclusively in uplake portions of both lakes. The percent of sunfish identified as hybrid was low in all zones of Mayo Reservoir (Table 4-14). Belews Lake had higher rates of sunfish hybridization, but this did not appear directly related to any thermal impact. Table 4-14. Percent pollution tolerance, trophic guild, and percent of hybrids for fish collected from electrofishing within three zones of Mayo Reservoir during spring 2020. Category B E G Lake -wide Tolerance rating Tolerant 14.2 0 2.9 6.3 Intermediate 85.4 100.0 97.1 93.6 Not defined 0.5 0.0 0.0 0.2 Trophic levels Piscivore 15.1 38.3 19.3 21.7 Insectivore 84.5 61.7 60.2 69.1 Omnivore 0.5 0.0 20.4 9.2 Percent hybrids Sunfish 0.5 0.0 0.0 0.2 The mean CPUE for centrarchid RIS identified for the Belews Lake demonstration were calculated from spring electrofishing in Mayo Reservoir. Alabama Bass and Redbreast Sunfish are not present in Mayo Reservoir, and therefore were not included in the comparisons. Largemouth Bass had a lake -wide mean CPUE of 55 fish/hr (Figure 4-30). This catch rate was above the 75th percentile for the eco-region (Brouder et al. 2009) and was comparable to the relative abundance of black bass in Belews Lake. Additionally, the mean lake -wide CPUE for Bluegill in Mayo Reservoir (107/hr, Figure 4-30) was similar to Belews Lake, whereas the mean CPUE for Redear Sunfish in Mayo Reservoir (51/hr, Figure 4-30) was higher than Belews Lake. This difference is more likely related to overall reservoir habitat characteristics (e.g., substrate type, localized bathymetry) than thermal impacts from BCSS. 71 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station CPUE (number/hr) 250 - 200 - 150 - 100 - 50 - 0 I Largemouth Bass Bluegill Redear Sunfish Figure 4-30. Mean catch rate (CPUE) by number of stock size and larger RIS collected from electrofishing in Mayo Reservoir during spring 2020. Error bars are 90% confidence intervals. The length frequencies of RIS in Mayo Reservoir were similar to those in Belews Lake. Largemouth Bass size structure in Mayo Reservoir was skewed towards larger individuals, especially those 380-510 mm, whereas Bluegill size structure was skewed towards individuals <150 mm (Figure 4-31). These skewed distributions, as well as the length distribution of Redear Sunfish, were similar to patterns documented in Belews Lake. Overall, the CPUE and size structure distributions of centrarchid RIS in Mayo Reservoir suggest no impact to these fish populations in Belews Lake related to the thermal discharge of BCSS. 72 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station c a) 2 E 30 - 25 - 20 - 15 - 10 - Largemouth Bass 50 40 30 20 10 0 60 50 40 100 200 300 400 500 30 20 10 0 Redear Sunfish Bluegill 40 80 120 160 200 240 280 Total length (mm) Figure 4-31. Length -frequency of centrarchid RIS collected from electrofishing in Mayo Reservoir during spring 2020. The mean CPUE of Channel Catfish (0.8 fish/net-night) and Gizzard Shad (2.7 fish/net-night) in Mayo Reservoir during fall gill netting (Figure 4-32) was lower than the mean CPUE in Belews Lake. The lower relative abundance of these two species in Mayo Reservoir may be related to interspecific competition. Mayo Reservoir had abundant and diverse assemblage of native bullheads as well as an abundant Golden Shiner Notemigonus crysoleucas population. Both taxa have been captured in Belews Lake, but their abundances have been greatly reduced over the past decades. This community shift is due in part to the introduction of Flathead Catfish Pylodictis olivaris, which has affected fish communities without thermal effluent elsewhere (Thomas 1993, Bonvechio et al. 2009, Baumann and Kwak 2011, Kwak et al. 2011, Dobbins et al. 2012). 73 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station CPUE (fish/net-night) 7 6- 5- 4- 3- 2- 0 Channel Catfish Gizzard Shad Figure 4-32. Mean catch rate (CPUE) by number of stock size and larger RIS collected from gill netting in Mayo Reservoir during fall 2020. Error bars are 90% confidence intervals. The size structure of Channel Catfish in Mayo Reservoir (Figure 4-33) was similar to that documented in Belews Lake. However, Gizzard Shad in Mayo Reservoir (Figure 4-33) were more skewed toward smaller individuals compared to Belews Lake. These differences may be related to variations in recruitment, growth rates, or mortality between the two reservoirs. These dynamic rate functions of Gizzard Shad have been linked to reservoir size, morphometry, turbidity, and productivity (Michaletz 2012, 2017). Regardless, the differences in size structure between the two reservoirs are not indicative of negative impacts from operations of BCSS. 74 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 20 18 - 16 14 12 - 10 - 8- 6- 4- 2- 0 Channel Catfish 100 200 300 400 500 600 30 - 25 - 20 - 15 - 10- 5- 0 Gizzard Shad 200 250 300 350 400 Total length (mm) Figure 4-33. Length -frequency of RIS collected from gill netting in Mayo Reservoir during fall 2020. The mean relative weight of Largemouth Bass and Channel Catfish in Mayo Reservoir were good with both at 96 (Figure 4-34). However, the mean relative weight of Bluegill (74), Redear Sunfish (73), and Gizzard Shad (82) were poor (Figure 4-34). These patterns, especially when combined with CPUE and size structure data, suggest Mayo Reservoir had a high density of RIS prey fish. In addition, a large number of the forage fish Golden Shiner were also captured in Mayo Reservoir. This high density of forage fish likely led to the poor condition and slow growth. In turn, the high abundance of prey allowed for good condition, faster growth, and a larger size structure of the predatory species (e.g., Largemouth Bass). The differences between Mayo Reservoir and Belews Lake were related to overall reservoir dynamics and fish community rather than any thermal impacts from operations at BCSS. 75 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Relative weight Figure 4-34. Condition (relative weight) of RIS in Mayo Reservoir for fish collected during fall 2020. The horizontal line represents the median for each species, the boxes represent the 25th and 75th percentile, and the whiskers show 10th and 90th percentiles. During this study, 24 species of fish (and one hybrid) from eight families were captured in Belews Lake, compared to 23 species of fish (and one hybrid) from six families were captured in Mayo Reservoir (Table 4-15). Only one family (Esocidae) was not represented in Belews Lake, while three families (Moronidae, Percidae, and Poeciliidae) were not captured in Mayo Reservoir. Shannon's diversity index (H) was higher for Mayo Reservoir than Belews Lake, despite Belews Lake having a slightly higher species richness (Table 4-15). The difference in diversity between the two reservoirs was related to the dominance of a few species in Belews Lake compared to Mayo Reservoir. The RIS comprised 91% of fish captured in Belews Lake compared to 74% of fish in Mayo Lake. Additionally, Bluegill were the most common species in both reservoirs, and they comprised 58% of fish captured in Belews Lake compared to only 40% in Mayo Reservoir. 76 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 4-15. Numbers of each species captured in Belews Lake and Mayo Reservoir during quarterly sampling with electrofishing and gill nets during 2020. Species richness and Shannon's diversity index (H) are also shown. Family Catostomidae Species Creek Chubsucker Golden Redhorse Notchlip Redhorse Quillback White Sucker Centrarchidae Alabama Bass Black Crappie Bluegill Green Sunfish Largemouth Bass Pumpkinseed Redbreast Sunfish Redear Sunfish Sunfish (Hybrid) Warmouth White Crappie Clupeidae Alewife Blueback Herring Gizzard Shad Threadfin Shad Cyprinidae Common Carp Creek Chub Esocidae Ictaluridae Moronidae Percidae Poeciliidae Golden Shiner Grass Carp Satinfin Shiner Chain Pickerel Blue Catfish Brown Bullhead Channel Catfish Flat Bullhead Flathead Catfish Snail Bullhead White Catfish Yellow Bullhead White Perch Yellow Perch Eastern Mosquitofish Number of species Shannon's H Scientific Name Erimyzon oblongus Moxostoma erythrurum Moxostoma collapsum Carpiodes cyprinus Catostomus commersonii Micropterus henshalli Pomoxis nigromaculatus Lepomis macrochirus Lepomis cyanellus Micropterus salmoides Lepomis gibbosus Lepomis auritus Lepomis microlophus Lepomis sp. Lepomis gulosus Pomoxis annularis Alosa pseudoharengus Alosa aestivalis Dorosoma cepedianum Dorosoma petenense Cyprinus carpio Semotilus atromaculatus Notemigonus crysoleucas Ctenopharyngodon idella Cyprinella analostana Esox niger Ictalurus furcatus Ameiurus nebulosus Ictalurus punctatus Ameiurus platycephalus Pylodictis olivaris Ameiurus brunneus Ameiurus catus Ameiurus natalis Morone americana Perca flavescens Gambusia holbrooki Belews Lake 0 0 0 1 0 1,325 94 6,301 159 470 2 503 501 218 22 17 20 0 471 243 16 0 2 1 16 0 4 0 267 0 16 0 0 0 80 16 3 25 1.61 Mayo Reservoir 6 1 21 0 12 0 119 1,613 151 530 0 0 467 5 41 0 0 24 351 3 2 1 141 0 0 160 0 19 36 222 0 47 61 12 0 0 0 24 2.05 77 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.7 Other Vertebrate Wildlife Wildlife observations associated with the Belews Creek Steam Station 316(a) studies were conducted for aquatic wildlife species or species that use Belews Lake for activities such as foraging or other life functions. Observations were conducted during the late summer period in the heated effluent discharge area. Ten different taxa of non -fish vertebrate wildlife were observed in the Belews Creek Steam Station discharge area on Belews Lake during summer 2020. All animals were engaging in natural behaviors that included the use of the thermally influenced water in some instances (Table 4-16). Table 4-16. Location, behavior, and number of aquatic vertebrate wildlife observed in the Belews discharge arm (2016-2020). Event (Date) 1-hr survey (8/5/2020) Habitat formers survey (9/16/20) Common Name Double -Crested Cormorant Canada Goose Osprey Great Blue Heron Killdeer Scientific Name Phalacrocorax auritus Branta canadensis Pandion haliaetus Ardea herodias Charadrius vociferous Behavior Foraging Flying/Swimming Flying/Foraging Flying Calling Number 1 15 3 1 1 Wood Duck Common Loon Great Egret Belted Kingfisher Double -Crested Cormorant Great Blue Heron Unidentified Turtle Aix sponsa Gavia immer Ardea alba Ceryle alcyon Phalacrocorax auritus Ardea herodias n/a Flying Swimming Wading Flying Flying Flying Swimming 4 4 1 1 5 1 1 4.8 Endangered Species A review of the USFWS IPaC tool for Belews Lake and the area surrounding BCSS was performed to determine the potential presence of federally listed aquatic species belonging to the lentic community. This search resulted in the identification of two federally endangered aquatic species, one freshwater mussel, the James Spinymussel (Pleurobema collina) and one fish, the Roanoke Logperch (Percina rex) as having the potential to occur near BCSS. Both species inhabit the Dan River watershed, although neither have been documented in Belews Lake. The surveys performed in 2020, in support of this demonstration documented two species of mussel (Eastern Elliptio and Paper Pondshell), and no darter species in Belews Lake. Furthermore, the habitats of both listed species are associated with rivers and streams, and not lacustrine systems like Belews Lake (Federal Register 2008; Hester and Smith 2007). Similarly, the same IPaC tool was used to assess the potential presence of federally listed aquatic vertebrate species. The search resulted in one endangered mammalian species (Gray Bat, Myotis grisencens), one threatened mammalian species (Northern Long -Eared Bat, Myotis septentrionalis), and one threatened reptilian vertebrate species (Bog Turtle, Clemmys muhlenbergii). The Northern Long - Eared Bat and the Gray Bat is not aquatic and therefore would not be directly affected by Belews Creek Steam Station thermal impacts. The Bog Turtle is semi -aquatic and predominately occurs in wetlands, bogs, or habitats with slow moving water and soft muddy bottoms (USFWS 2001). These habitats do not occur in Belews Lake near the Belews Creek Steam Station discharge, and there are no known 78 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station populations within the Belews Lake - Belews Creek Steam Station Project boundary (Duke Energy 2019 Gismo EHS-Viewer Data Base). 5 Balanced and Indigenous Assessment This assessment demonstrated that current BCSS operations will ensure the survival, reproduction, development, and growth of RIS. The temperature analysis indicated that the potential for mortality associated with high discharge temperatures is negligible under the extreme conditions captured for BCSS and would be even less so under typical seasonal weather and lake -level conditions. The distribution and density of habitat formers across Belews Lake was conducive to the reservoir morphology, yielding more vegetation in the shallow portions of the reservoir (Zones B and F). Conversely, deeper portions of the reservoir yielded poorly vegetated areas (Zones C, D, and E). However, there was no apparent trend between locations and density of vegetated points and the zones of thermal influence. Results from the mussel surveys indicated that 2 species were present in Belews Lake. Adult Eastern Elliptio were found in five of the six zones, and young individuals were also located in Zones C, D, and E, which suggests that recruitment has occurred. In addition, the high abundance of Eastern Elliptio indicated that suitable habitat conditions are present in Belews Lake. The assessment of the BCSS thermal plume demonstrate that continued operations will assure the propagation and protection of aquatic vegetation, mussel communities, and the BIC represented by the RIS that could reside in the thermally influenced zones. This demonstration revealed that the Belews Lake fish community was composed mostly of indigenous species (73% native species) expected from a reservoir located in the North Carolina Piedmont (Fowler 1945; Barwick 2002). During 2020, 24 distinct species of fish and one hybrid complex were collected from the six survey zones during quarterly electrofishing and gillnetting. The CPUE of fish during spring 2020 was similar to those documented since 2005 (Duke Energy 2015; Barwick and Harrell 1997). Species diversity was similar in all zones of the lake, regardless of thermal influence. The fish community found in the zones of Belews Lake with the most thermal influence from BCSS (Zones A, B, and C) encompassed multiple trophic guilds (i.e., insectivores, omnivores, and piscivores) supporting a balanced fish community. Additionally, fish captured in these zones had similar proportions of pollution tolerance to other zones of the lake, and no zones were dominated by pollution -tolerant species. Although non -indigenous species such as Alabama Bass and Redear Sunfish were abundant in thermally -influenced zones, they were caught throughout Belews Lake. Some indigenous species (e.g., Largemouth Bass) have experienced reduced CPUE compared to historical surveys which may be a result of an altered fish community related to the introduction of nonnative species (e.g., Alabama Bass, Blue Catfish, and Flathead Catfish). Regardless, the catch was dominated by species native to the watershed. The RIS had size structures in all zones indicative of multiple age classes including both age- 1 fish and the largest mature individuals. The size structures suggest these populations have the capacity to be sustained in all zones of the lake as lake conditions change throughout the year. Based on the diversity and numbers of individuals in the littoral fish community during spring sampling and the condition of RIS collected during fall sampling, Belews Lake supports a balanced and indigenous fish community. Duke Energy has complied with the current thermal compliance point which states the daily average ambient water temperatures shall not exceed 32 °C at the dam discharge. Under these conditions, the 79 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station current study has shown that survival, reproduction, development, and growth of RIS have not been appreciably reduced due to operations. Additionally, the BCSS thermal plume has not blocked or inhibited access to any potential spawning habitat, spawning activities, or the development of early juveniles of RIS and the BIC. Consequently, the current thermal limits and BCSS operations have ensured the protection of a BIC in Belews Lake. 6 References Barwick, D.H., Harrell, R.D. 1997. 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Food habits of co- occurring populations of Largemouth Bass and Spotted Bass in two New Mexico reservoirs. Journal of Freshwater Ecology 20:37-46. Sammons, S. M., and P. W. Bettoli. 1999. Spatial and temporal variation in electrofishing catch rates of three species of Black Bass (Micropterus spp.) from Normandy Reservoir, Tennessee. North American Journal of Fisheries Management 19:454-461. 82 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Schindler, DW, RE Hecky, DL Findlay, MP Stainton, BR Parker, MJ Paterson, KG Beaty, M Lyng, SEM Kasian. 2008. Eutrophication of lakes cannot be controlled by reducing nitrogen input: results of a 37-year whole -ecosystem experiment. Proceedings of the National Academy of Sciences of the United States of America, 105:11254-11258. The Weather Company (TWC). 2020. Weather conditions information web site: Piedmont Triad International Airport Station Historical Weather. (Accessed: 1/4/2020) Web address: http://www.wunderground.com/weather/KGSO. Thomas, M. E. 1993. Monitoring the effects of introduced Flathead Catfish on sport fish populations in the Altamaha River, Georgia. Proceedings of the Annual Conference Southeastern Association of Fish and Wildlife Agencies 47:531-538. United States Geological Survey (USGS). 2020. Water resources data, National Water Information System web site: USGS water for North Carolina. (Accessed: 1/4/2021) Web address: http://waterdata.usgs.gov/nc/nwis. USEPA. 1978. A Compendium of Lake and Reservoir Data Collected by the National Eutrophication Survey in Eastern, North-Central, and Southeastern United States. Working Paper No. 475. Van Horn, S. L. 1978. Development of the sport fish potential of an industrial cooling lake. North Carolina Wildlife Resource Commission, Division of Inland Fisheries. Raleigh, NC. Wege, G. J. and R. O. Anderson. 1978. Relative weight (Wr): a new index of condition for largemouth bass. Pages 79-91 in G. D. Novinger and J. G. Dillard, editors. New Approaches to the Management of Small Impoundments. American Fisheries Society, North Central Division, Special Publication 5, Bethesda, Maryland. Weiss, C.M. and E.J. Kuenzler. 1976. The trophic state of North Carolina lakes. Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina at Chapel Hill. Report No. 119. Weiss, C. M. and T. P. Anderson. 1978. Belews Lake: a summary of a seven year study (August 1970- June 1977) to assess environmental effects of a coal-fired power plant on a cooling pond. ESE No. 475. Department of Environmental Sciences and Engineering, UNC Chapel Hill. Chapel Hill, NC. Willis, D. W., B. R. Murphy, and C. S. Guy. 1993. Stock density indices: development, use, and limitations. Reviews in Fisheries Science 1:203-222. Wilson, D., F. R. Cole, J. Nichols, R. Rudran, and M. Foster, editors. 1996. Measuring and Monitoring Biological Diversity. Standards Methods for Mammals. Smithsonian Institution Press. Washington and London. Yurk, JJ, and JJ Ney. 1989. Phosphorous -fish community biomass relationships in southern Appalachian reservoirs: can lakes be too clean for fish? Lake and Reservoir Management 5:89-90. Zale, A. V., D. L. Parrish and T. M. Sutton, editors. 2012. Fisheries Techniques, third edition. American Fisheries Society. Bethesda, MD. 83 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Appendices 84 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Appendix A Final BCSS 316(a) Study Plan A-1 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Contents 1 Introduction A-4 1.1 Background A-4 1.2 Environmental Monitoring History A-4 1.3 Thermal Permit History A-5 2 Purpose A-5 3 Study Goals A-5 4 Study Plan A-6 4.1 Thermal Analysis A-6 4.2 Limnology A-7 4.3 Habitat Formers A-7 4.4 Planktonic Communities A-7 4.5 Benthic Macroinvertebrate Community A-7 4.5.1 Mussel Community Survey A-7 4.6 Fish Community A-7 4.6.1 Electrofishing A-8 4.6.2 Gill NetsA-8 4.6.3 AnalysisA-8 4.7 Other Vertebrate Wildlife A-8 4.8 Endangered Species A-9 5 Reference Lake A-9 6 Data Management A-10 7 Study Timeline and Reporting A-10 8 References A-10 9 Figures and Tables A-13 Tables Table 1. Belews Creek Steam Station 316(a) Demonstration Study Plan Summary. Table 2. Belews Lake Water Quality and Water Chemistry Monitoring Variables. A-15 Table 3. Representative Important Species (RIS) in Belews Lake. A-15 A-2 A-14 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Figures Figure 1. Belews Lake 316(a) study areas (labeled with bold letters and delineated with solid black lines) and sample locations. A-13 A-3 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 1 Introduction 1.1 Background Belews Creek Steam Station (BCSS) is a two -unit, coal-fired electric generating plant located in Stokes County, North Carolina, approximately 15 miles northeast of Winston-Salem. The station sits adjacent to Belews Lake, an impoundment of Belews Creek, West Belews Creek and East Belews Creek, part of the Dan River (Roanoke) watershed (Figure 1). The reservoir, impounded primarily to supply condenser cooling water (CCW), first reached full pond in 1973 after the dam was completed in 1970. BCSS Unit 1 began commercial operation in August 1974 followed by Unit 2 operation in December 1975. Each 1,245.6-megawatt Unit is cooled by CCW pumped at a maximum rate of 33.1 m3/s (1,170 cubic feet per second [cfs]). Historically, BCSS has been operated as a baseload generating station. Belews Lake has a surface area of 15.63 km2 and is relatively deep for a piedmont reservoir (14.6 m mean depth). The watershed, however, is comparatively small (197 km2) with an average drainage flow of 2.8 m3/s (99 cfs). Low inflows, combined with evaporative loss from the station results in a long average retention time of 1,500 days. The shoreline is mostly steep, buffered primarily by undeveloped forest with sparse residential development. Much of the nutrient load from the watershed is sequestered in the upper reaches of Belews Lake. As a result, there is a productivity gradient from the upper Lake (Areas 1,1 and K) to the lower Lake (Areas B, C, D and E; Figure 1). 1.2 Environmental Monitoring History Duke Energy has performed or sponsored environmental monitoring on Belews Lake since dam construction was completed in 1970. The initial study, performed from 1970 —1977, included three years prior to full pond being reached, one year at full pond before station operation, and three years after the station began operation (Weiss and Anderson 1978). This study evaluated water quality and chemistry, phytoplankton, zooplankton, and benthic macroinvertebrates. The North Carolina Wildlife Resource Commission (NCWRC) surveyed the Belews Lake fishery for sport fish potential during the same time period, from 1971-1978 (Van Horn 1978). By 1975, substantial declines in fish populations and recruitment became evident in lower Belews Lake, and it was determined that selenium loading from BCSS ash basin sluicing into the lake, exacerbated by the long retention time, was inhibiting fish reproduction (Harrell et al. 1978). Environmental studies were restructured to monitor effects of selenium on Belews Lake biota and water quality. These lake recovery sampling programs evolved over time and new sampling programs were created when BCSS redirected its regulated ash basin discharge to the Dan River in October 1985. Belews Lake biota began to recover once this redirection occurred (Duke Power Company 1996). Over the past several decades, environmental monitoring on Belews Lake has focused on water quality/chemistry, benthic macroinvertebrates, and fisheries. Water quality and water chemistry samples have been collected on at least a semi-annual basis since 1977. Annual cove rotenone surveys were performed from 1977 —1994 to sample littoral fish populations (Duke Power Company 1996). Semi-annual or annual electrofishing surveys began in 1983 and continue through the present. From 1991-2016, benthic macroinvertebrate community samples were collected. Starting in 1996, lake environmental data were submitted to North Carolina Department of Environmental Quality (NCDEQ) during each National Pollutant Discharge Elimination System (NPDES) permit cycle. The NCWRC has also conducted several fisheries surveys and research projects on Belews Lake over the years (Hining 2003, 2005a, 2005b; Hodges 2012). A-4 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 1.3 Thermal Permit History BCSS operates under NPDES permit no. NC0024406. The initial permit was issued by the State of North Carolina in 1970 prior to development of the Clean Water Act (CWA) and associated §316(a) requirements for a thermal variance. The initial permit granted BCSS a temperature variance that stated daily average ambient water temperatures shall not exceed 32°C at the dam discharge as a result of BCSS operations. North Carolina's Division of Water Resources (DWR) issued BCSS a new NPDES permit in 2012, and stated in Section A. (15.), "The thermal variance granted by the State of North Carolina terminates on expiration of the NPDES permit. Should the permittee wish a continuation of its thermal variance beyond the term of this permit, reapplication for such continuation shall be submitted in accordance with 40 CFR Part 125, Subpart H and Section 122.21 (1)(6)...The temperature analysis and the balanced and indigenous study plan shall conform to the specifications outlined in 40 CFR Part 125 Subpart H and the Environmental Protection Agency's (EPA) draft 316a Guidance Manual, dated 1977." Upon review of the 2011-2015 BCSS 316(a) report submitted to the State in 2016, North Carolina Department of Environment and Natural Resources (NCDENR) commented that the report did not satisfy the 2012 permit requirements specified in Section A. (15.). To address these comments and continue operating under a thermal variance, the new 2019 NPDES five-year permit for BCSS requests in Section A. (24.) a 1-year comprehensive 316(a) Demonstration study, performed in accordance to specifications in 40 CFR Part 125 Subpart H and the EPA's 1977 draft 316(a) Guidance Manual. 2 Purpose In accordance with NPDES permit no. NC0024406, Section A. (24.), Duke Energy will conduct an initial, comprehensive one-year Demonstration study on Belews Lake to support a request for a thermal variance for BCSS under §316(a) of the CWA. The purpose of this Study Plan is to describe the thermal analysis as well as the biological sampling required that will give Duke Energy the opportunity to apply for and acquire an alternative effluent thermal limitation. 3 Study Goals The two primary goals of this 316(a) study are to: 1. Perform a thermal analysis of Belews Lake and 2. Demonstrate the protection and propagation of a balanced, indigenous community (BIC) in Belews Lake through biological surveys. A-5 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Data collected during this study will be evaluated against four primary BIC criteria defined in 40 CFR 125.71. The four criteria state that BICs are biotic communities typically characterized by: a. Having diversity and representative trophic levels within expectations, b. The ability to self -sustain through successful reproduction and recruitment over seasonal changes, c. Having adequate food items, and d. A lack of domination by pollution tolerant species. Representative Important Species (RIS) have been chosen and will be used as part of the assessment due to numerous species being present in Belews Lake. The RIS will be used to indicate a BIC exists within Belews Lake. The NCWRC and the NCDEQ support the species selected as RIS (Kin Hodges, personal communication, June 29, 2018 and Jeff DeBerardinis, personal communication, April 8, 2019). 4 Study Plan The following describes the study components of the proposed Belews Lake 316(a) Demonstration study. Table 1 displays the different sampling programs, locations and frequencies for the year -long study period. 4.1 Thermal Analysis A rigorous temperature sampling program in Belews Lake was conducted in 2017 (January — December) in anticipation of the upcoming temperature analysis requirement within the 316(a) Demonstration. Monthly water quality profiles (measurements from surface to bottom at 1 m intervals) were collected at twelve locations in Belews Lake, along with continuous in situ temperature profile loggers at five of the locations (Figure 1). These continuous loggers recorded temperature hourly and were deployed from the surface down to 20 m (or lake bottom, whichever was less) at 2 m intervals, with the exception of location 416.0 near the dam. Continuous loggers were deployed deeper (down to lake bottom, 36 m) at 416.0 to document thermal stratification formation during summer and subsequent mixing in autumn. Monthly profile data and hourly temperature data collected from the continuous loggers, along with historical profile data and hourly BCSS intake and discharge temperatures (provided through internal Duke Energy sources) will provide the data required to perform a thermal analysis of Belews Lake. Vertical profiles, time -series graphs, surface/transect contour graphs, thermal plume extent (i.e., ambient boundary), degree of stratification, intake temperature and other potential parameters may be used to display different seasonal conditions in the lake. Species -specific heat -tolerance data can be overlaid with these displays (e.g., surface and cross -sectional avoidance areas by species), which will help determine if any fish migrations barriers may exist related to the thermal discharge. Spatial analysis of the surface thermal plume in Belews Lake during winter and summer worst -case scenarios will be produced from archived satellite imagery. Satelytics Inc. will provide these images from Landsat 7 Band 6 (thermal; X = 10.4-12.5 µm) satellites. Thermal resolution will be reported at 1°C. A-6 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.2 Limnology Data from Duke Energy's existing in situ water quality and water chemistry monitoring program will be incorporated into the 316(a) Demonstration to address any potential water quality/chemistry interactions with the thermal effluent (e.g., dissolved oxygen, chlorophyll a, etc.) and how those interactions may affect the Lake's biotic community. A list of variables in the sampling program can be found in Table 2. 4.3 Habitat Formers One qualitative (presence/absence) habitat former (aquatic vegetation) survey will be conducted in the summer during the year -long comprehensive study. Presence and spatial distribution will be recorded for all visible aquatic vegetation species (submerged, floating and emergent). Observations will be mapped and overlaid with thermal plume displays to determine if aquatic vegetation distributions are influenced by the thermal discharge. 4.4 Planktonic Communities Phytoplankton and zooplankton are low potential impact (LPI) biotic categories. Narrative assessments of these components will be made and included within the framework of the Belews Lake BIC utilizing scientific literature and historical planktonic data collected from Belews Lake (Weiss and Anderson 1978). The validity of using a narrative approach can be found in the most recent 316(a) review by Coutant (2013). 4.5 Benthic Macroinvertebrate Community Like phytoplankton and zooplankton, benthic macroinvertebrates are LPI. The narrative assessment will include assessed using scientific literature and historical data (Duke Energy 2005, 2011, 2015; Duke Power Company 1996, 2000; Weiss and Anderson 1978). 4.5.1 Mussel Community Survey Qualitative (timed/distance) mussel surveys will be conducted once during the Demonstration period according to Duke Energy procedure FSH-867.0 (on file with NCDEQ). All native mussel species will be considered RIS (Table 3). The goal will be to complete two timed surveys within each fisheries study location (12 total surveys, see section 4.6 below; Figure 1; Table 1), each survey encompassing 4.0 person -hours of search time or at least a distance of 200 m. Survey site locations, substrate descriptions, species identifications, relative abundance (Catch -Per -Unit -Effort [CPUE]), individual length measurements and survey technique (e.g., tactile search using surface air supply, snorkeling and bathyscope) will be recorded for each location site. Descriptive statistics will be used to analyze results. Results will be mapped using GIS techniques. 4.6 Fish Community Fisheries surveys will be performed during the Demonstration to evaluate for BIC. As stated above, a BIC should be diverse and contain different trophic levels, be self-sustaining year to year, not be dominated by pollution -tolerant species and contain adequate food items. Fish species selected to be RIS are listed in Table 3. A-7 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 4.6.1 Electrofishing Boat electrofishing surveys will be conducted according to standard fisheries methods (Zale et al. 2012) and Duke Energy procedure FSH-250.05. Sampling will be done quarterly (every three months), and four timed transects (approximately 1000 seconds of effort per transect) will be performed in each study location, totaling 24 transects lake wide (Figure 1; Table 1). Transects will be sampled during the day with a Smith -Root GPP Electrofisher mounted on a Smith -Root boat, using pulsed DC current. Transects will be established parallel to the shoreline and be designed to ensure no overlap with other transects will occur. Boat crews will consist of a driver and two netters on the bow. Species identification, enumeration and individual total length (nearest millimeter) and weight (nearest gram) will be recorded for fish collected in each transect. Fish will also be inspected for parasites and any deformities. If fish are not identifiable in the field they will be preserved and taken back to the lab for identification. Water quality measurements (temperature, conductivity, dissolved oxygen and pH) will be taken at each transect with a calibrated probe to evaluate environmental conditions at the time of collection. 4.6.2 Gill Nets Experimental gill nets will be deployed according to Duke Energy procedure FSH-252.03 during each quarterly electrofishing survey to select for bottom -orienting and pelagic fish. Gill nets will be 8' deep and 100' long with four incremental, 25' sections per net (1", 2", 3" and 4" monofilament stretch length). Two gill nets will be set in each study location for two net -nights, totaling four net -nights every quarter per location (24 total net -nights lake wide). Every study location will have three or four potential gill net locations (Figure 1; Table 1), two of which will be randomly selected each net -night. Nets will be set on lake bottom approximately perpendicular to the shoreline with the 1" stretch section nearest to shore. After each net -night the fish will be removed and the net re -set or stored on the boat. Individual fish will be identified, enumerated, measured and inspected using the same methods described for fish collected during electrofishing (see above). 4.6.3 Analysis Data analysis will consist of total taxa numbers and biomass (fish only), mean CPUE calculations, spatial comparisons of RIS, length distributions, species pollution tolerance, trophic guild and hybrid complexes. Comparisons will be made among locations using one-way ANOVA with the conservative Bonferroni multiple -comparison procedure (a = 0.1) and analysis of similarity (ANOSIM) and related community level analyses. Simple means, standard deviations and ranges may also be reported. Biological community indices such as Shannon's Diversity Index and Species Richness will be an additional data assessment tool. Primer 7 and Sigma Plot will be utilized for biological data analyses, and GIS will primarily be utilized for graphic displays. 4.7 Other Vertebrate Wildlife In addition to aquatic biota, Duke Energy will conduct observations regarding "other vertebrate wildlife" (wildlife) that are associated with aquatic habitats and/or rely on the waters for foraging, reproduction, and other life functions (e.g., waterfowl, bald eagles, aquatic mammals, amphibians). According to the USEPA 1977 316(a) Technical Guidance Document, most sites in the United States will likely be considered ones of LPI for other vertebrate wildlife simply because thermal discharge plumes should not generally impact large or unique populations of wildlife (e.g., waterfowl concentrations, A-8 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station eagle wintering areas). Exceptions to sites classified as LPI would be those few sites where the discharge might affect protected, RIS, or threatened and endangered wildlife. Observations for wildlife will be conducted by qualified Duke Energy staff at sampling sites similar in general location to those being conducted for the fisheries study component (Braun 2005; Heyer et. al., 1994; Wilson et.al., 1996). The observations will also be conducted in the same time period (i.e., month, season) as the fisheries fieldwork. Observations will be augmented by literature reviews of pertinent information (e.g., USFWS listed species county list, USFWS Information for Planning and Construction database, facility -specific reports) which will enable Duke Energy to prepare rationale regarding why the site should be considered one of low potential impact or an exception to that. Observations will be conducted for aquatic wildlife species or species that use the Belews aquatic system during activities such as foraging for fish or other life function activities. During this Demonstration, Duke Energy will not be documenting wildlife species that do not fit the criteria mentioned above (e.g., white-tailed deer, most songbirds and wild turkeys). 4.8 Endangered Species Wildlife surveys (Section 4.7 above) will include an assessment of presence/absence of threatened and endangered terrestrial species that may inhabit or potentially use the area near BCSS (e.g., bald eagle). Information regarding the protected and federally listed terrestrial species will be obtained via the United States Fish and Wildlife Service's (USFWS) Stokes, Rockingham, and Forsyth Counties, NC county -wide list (USFWS 2014). Mussel surveys will also be utilized to detect any threatened and endangered mussel species. Scientific literature, federal and state surveys and listings, and Natural Heritage Program database element occurrences will be reviewed or queried for other protected aquatic species that may occur in Belews Lake. Lastly, the NCWRC will be consulted for additional input. 5 Reference Lake If available, additional reference locations are valuable in a 316(a) Demonstration (Coutant 2013). Mayo Reservoir is an impoundment of Mayo Creek in Person County, North Carolina, approximately 65 miles ENE of Belews Lake. Reaching full pond in 1983, it was originally created to supply make-up cooling water to Mayo Steam Electric Plant (MP). Although Mayo Reservoir is smaller and shallower than Belews Lake (1,133 ha compared to 1,563 ha and average depth of 9.0 m to 14.6 m, respectively), it is a good candidate as a reference lake for several reasons listed below: 1. Mayo Reservoir receives little to zero thermal input from MP due to cooling towers, 2. has a similar but less severe legacy issue with selenium loading to the lake, 3. is in the same basin as Belews Lake (Roanoke River basin), 4. has similar productivity and nutrient load to Belews Lake (low to moderate), 5. has a similarly long retention time (1,100 days compared to 1,500 days for Belews Lake), 6. is buffered by similar land use patterns (mostly undeveloped forest), and A-9 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 7. was recently found to have a "relatively balanced and stable fish community" by NCDWR (2018). Consistent monitoring has been occurring on Mayo Reservoir since 1983 and continues through the present (Duke Energy Progress 2017). Water quality and water chemistry are currently collected bi- monthly (six times per year) and fisheries data are collected through shoreline electrofishing four times a year in April, May, October and November. In addition, experimental gill nets identical to those used in Belews Lake will be deployed during fisheries surveys in 2019 and 2020. These environmental reference data from Mayo Reservoir will be compared with data collected from Belews Lake during the 316(a) Demonstration. Indices to be compared between lakes may include pollution tolerant species percentages, species trophic level percentages, Species Richness, Shannon Diversity Index and others, as well as water quality and chemistry parameters. 6 Data Management All data collected by Duke Energy for the BCSS 316(a) Demonstration will be digitally recorded and uploaded into Duke Energy's EQuIS database for retrieval and analysis. Internal QA/QC processes will be established to ensure accuracy of data being submitted to the EQuIS database. 7 Study Timeline and Reporting The BCSS NPDES permit has an effective date of March 25, 2019, with a due date of August 22, 2019 for the final draft of the 316(a) Demonstration Study Plan. The BCSS 316(a) Demonstration will commence January 1, 2020 and will be conducted for one year. According to the NPDES Permit, study results will be presented in a report to NCDEQ within 120 days of monitoring completion (May 1, 2021) for application of a 316(a) variance. 8 References Braun, C. E., editor. 2005. Techniques for Wildlife Investigations and Management. Sixth edition. The Wildlife Society. Bethesda, MD. Coutant, C. 2013. Considerations and requirements for biological determinations related to thermal discharges. Special Report No. 13-02. National Council for Air and Stream Improvement. August 2013. Duke Energy. 2005. Assessment of balanced and indigenous populations in Belews Lake. NPDES No. NC0024406. Duke Power Company, Huntersville, NC. A-10 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Duke Energy. 2011. Assessment of balanced and indigenous populations in Belews Lake. NPDES No. NC0024406. Duke Power Company, Huntersville, NC. Duke Energy. 2015. Assessment of balanced and indigenous populations in Belews Lake. NPDES No. NC0024406. Duke Power Company, Huntersville, NC. Duke Energy Progress. 2017. Mayo Steam Electric Plant 2016 Environmental Monitoring Report. NPDES No. NC0038377. Duke Energy Progress, Raleigh, NC. Duke Power Company. 1996. Assessment of balanced and indigenous populations in Belews Lake. NPDES No. NC0024406. Duke Power Company, Huntersville, NC. Duke Power Company. 2000. Assessment of balanced and indigenous populations in Belews Lake. NPDES No. NC0024406. Duke Power Company, Huntersville, NC. Harrel, R. D., R. L. Fuller and T. J. Edwards. 1978. An investigation of the fish community of Belews Lake North Carolina. DukePWR/78-07. Duke Power Company, Charlotte NC. Heyer, W. R., M. Donnelly, R. McDiarmid, L. Hayek, and M. Foster, editors. 1994. Measuring and Monitoring Biological Diversity. Standards Methods for Amphibians. Smithsonian Institution Press. Washington and London. Hining, K. 2003. Characteristics of the black and white crappie populations in Belews Lake, 2001-2002. North Carolina Wildlife Resource Commission, Division of Inland Fisheries. Raleigh, NC. Hining, K. 2005a. Characteristics of the black and white crappie populations in Belews Lake, 2004. North Carolina Wildlife Resource Commission, Division of Inland Fisheries. Raleigh, NC. Hining, K. 2005b. Comparison of day and night electrofishing for largemouth and smallmouth bass in three North Carolina reservoirs. North Carolina Wildlife Resource Commission, Division of Inland Fisheries. Raleigh, NC. Hodges, K. 2012. Belews Lake largemouth bass survey, 2007-2009. North Carolina Wildlife Resource Commission, Division of Inland Fisheries. Raleigh, NC. NCDWR. Review of Progress Energy, Mayo Electric Generation Plant (NC 0038377) Environmental Monitoring Report (Letter). May 24, 2018. USFWS. 2014. Endangered Species, Threatened Species, Federal Species of Concern, and Candidate Species. New Hanover County, NC. Raleigh Ecological Field Office. Van Horn, S. L. 1978. Development of the sport fish potential of an industrial cooling lake. North Carolina Wildlife Resource Commission, Division of Inland Fisheries. Raleigh, NC. Weiss, C. M. and T. P. Anderson. 1978. Belews Lake: a summary of a seven year study (August 1970- June 1977) to assess environmental effects of a coal-fired power plant on a cooling pond. ESE A-11 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station No. 475. Department of Environmental Sciences and Engineering, UNC Chapel Hill. Chapel Hill, NC. Wilson, D., F. R. Cole, J. Nichols, R. Rudran, and M. Foster, editors. 1996. Measuring and Monitoring Biological Diversity. Standards Methods for Mammals. Smithsonian Institution Press. Washington and London. Zale, A. V., D. L. Parrish and T. M. Sutton, editors. 2012. Fisheries Techniques, third edition. American Fisheries Society. Bethesda, MD. A-12 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station 9 Figures and Tables IA 418.0 Belews Creek Steam Station— ❑ 0 0.5 1 2 Miles I I 1 1 0 0.75 1.5 3 Kilometers NA Sampling Locations: O Water Quality Profiles + Continuous Temp. Loggers • Electrofishing o Gill Nets o Mussels DUKE FNFRGY Figure 1. Belews Lake 316(a) study areas (labeled with bold letters and delineated with solid black lines) and sample locations. A-13 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 1. Belews Creek Steam Station 316(a) Demonstration Study Plan Summary. PROGRAM FREQUENCY LOCATION Limnology Water Quality Quarterly 416.0, 411.0, 412.0, 418.0, 410.5, 410.0, 408.0, 408.1, 408.2, 419.3, 419.2, 405.0 Water Chemistry 1" Quarter, 3rd Quarter 416.0, 411.0, 412.0, 418.0, 418.3, 410.0, 408.2, 419.3, 419.2, 405.0 Chlorophyll a 1St Quarter, 3rd Quarter 416.0, 411.0, 412.0, 418.0, 418.3, 410.0, 408.2, 419.3, 419.2, 405.0 Habitat Formers Aquatic Vegetation Once during summer Lake -wide littoral zone Mussel survey Once during summer 12 transects, 2 each at 6 locations 412.0, 418.0, 410.0, 408.0, 408.2, 419.2 Fisheries Electrofishing Gill nets Quarterly 24 transects, 4 each at 6 locations 412.0, 418.0, 410.0, 408.0, 408.2, 419.2 Quarterly 2x 100ft experimental nets at 6 locations 412.0, 418.0, 410.0, 408.0, 408.2, 419.2 Other Wildlife Observation Once during summer One observation at 6 location vicinities 412.0, 418.0, 410.0, 408.0, 408.2, 419.2 1 No metals collected except at locations D_3_418.3 and J_2_419.2. A-14 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table 2. Belews Lake Water Quality and Water Chemistry Monitoring Variables. Water Quality Water Chemistry Metals (total) Arsenic Copper Mercury (Low Level) Selenium Zinc Ions Calcium Chloride Magnesium Sulfate Temperature pH Dissolved oxygen Specific conductivity Chlorophyll a Nutrients Ammonia nitrogen Nitrite -nitrate nitrogen Total Kjeldhal nitrogen Total phosphorus Orthophosphate Total organic carbon Alkalinity (TIP) Total Hardness Physical Total dissolved solids Turbidity Table 3. Representative Important Species (RIS) in Belews Lake. Fish Common Name Gizzard Shad Channel Catfish Redbreast Sunfish Bluegill Redear Sunfish Spotted Bass Largemouth Bass Mussels Any native species collected Scientific Name Dorosoma cepedianum Ictalurus punctatus Lepomis auritus Lepomis macrochirus Lepomis microlophus Micropterus punctulatus Micropterus salmoides A-15 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Appendix B Real Statistics° Statistical Analysis B-16 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Winter Temperature Comparison by Zone Descriptive Statistics Zone A Zone C Zone D Zone E Zone F Mean 16.41791919 14.94394699 13.3216197 12.8579614 9.905342403 Standard Error 1.245978252 0.832379875 0.470511454 0.33369949 0.803201976 Median 15.72556317 14.38873656 12.86364382 12.65193527 10.06467958 Standard Deviation 2.158097637 1.441724236 0.814949745 0.577984471 1.391186632 Sample Variance 4.657385413 2.078568771 0.664143086 0.334066048 1.935400244 Skewness 1.295090518 1.475954236 1.730223021 1.40024021 -0.508638848 Range 4.146259889 2.718363335 1.423849222 1.099510753 2.768652374 Maximum 18.83722715 16.58073387 14.26253226 13.51072984 11.21 Minimum 14.69096726 13.86237054 12.83868304 12.41121909 8.441347626 Sum 49.25375758 44.83184097 39.96485911 38.57388419 29.71602721 Geometric Mean 16.32642851 14.89891484 13.3053726 12.84941644 9.838731689 Harmonic Mean 16.23849946 14.85534601 13.28951595 12.84099254 9.771098225 AAD 1.61287197 1.091191255 0.627275036 0.435178961 0.975996518 MAD 1.03459591 0.526366023 0.024960781 0.240716182 1.145320417 IQR 2.073129944 1.359181668 0.711924611 0.549755376 1.384326187 Multiplier 2.2 Zone A Zone C Zone D Zone E Zone F Min 14.69096726 13.86237054 12.83868304 12.41121909 8.441347626 Q1-Min 0.517297955 0.263183012 0.012480391 0.120358091 0.811665978 Med-Q1 0.517297955 0.263183012 0.012480391 0.120358091 0.811665978 Q3-Med 1.555831989 1.095998656 0.69944422 0.429397285 0.572660209 Max-03 1.555831989 1.095998656 0.69944422 0.429397285 0.572660209 Mean 16.41791919 14.94394699 13.3216197 12.8579614 9.905342403 Min 14.69096726 13.86237054 12.83868304 12.41121909 8.441347626 Q1 15.20826522 14.12555355 12.85116343 12.53157718 9.253013605 Median 15.72556317 14.38873656 12.86364382 12.65193527 10.06467958 Q3 17.28139516 15.48473522 13.56308804 13.08133255 10.63733979 Max 18.83722715 16.58073387 14.26253226 13.51072984 11.21 Mean 16.41791919 14.94394699 13.3216197 12.8579614 9.905342403 Grand Min 0 Outliers None None None None None B-17 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Shapiro -Wilk Test IIM Zone A Zone C Zone D Zone E Zone F W-stat 0.92280698 0.88877252 0.76314376 0.90470425 0.990161594 p-value 0.462295338 0.350604884 0.029248454 0.400635418 0.810250465 alpha 0.05 0.05 0.05 0.05 0.05 normal yes yes no yes yes Levene's Tests type p-value means 0.19552622 medians 0.762901942 trimmed 0.19552622 Factor Single 11 Factor DESCRIPTION Alpha 0.05 Group Count Sum Mean Variance SS Std Err Lower Upper Zone A 3 49.25375758 16.41791919 4.657385413 9.314770825 0.80289325 14.62896155 18.20687684 Zone C 3 44.83184097 14.94394699 2.078568771 4.157137543 0.80289325 13.15498934 16.73290463 Zone D 3 39.96485911 13.3216197 0.664143086 1.328286173 0.80289325 11.53266206 15.11057735 Zone E 3 38.57388419 12.8579614 0.334066048 0.668132097 0.80289325 11.06900375 14.64691904 Zone F 3 29.71602721 9.905342403 1.935400244 3.870800488 0.80289325 8.116384759 11.69430005 ANOVA Sources SS df MS F P value Eta-sq RMSSE Omega Sq Between Groups 71.89279621 4 17.97319905 9.293697143 0.002114399 0.788022367 1.760084955 0.688633818 Within Groups 19.33912713 10 1.933912713 Total 91.23192333 14 6.516565952 B-18 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station TUKEY HSD/KRAMER alpha 0.05 group mean n ss df q-crit Zone A 16.41791919 3 9.314770825 Zone C 14.94394699 3 4.157137543 Zone D 13.3216197 3 1.328286173 Zone E 12.8579614 3 0.668132097 Zone F 9.905342403 3 3.870800488 15 19.33912713 10 4.654 111111 Q TEST group 1 group 2 mean q-stat p-value Cohen d Zone A Zone C 1.473972206 1.835825879 0.698338205 1.059914565 Zone A Zone D 3.096299491 3.856427353 0.11887182 2.226509371 Zone A Zone E 3.559957797 4.433911728 0.04623 2.55992013 Zone A Zone F 6.512576792 8.111385657 0.001371947 4.683110692 Zone C Zone D 1.622327285 2.020601475 0.624833555 1.166594805 Zone C Zone E 2.085985591 2.598085849 0.405671525 1.500005564 Zone C Zone F 5.038604586 6.275559778 0.008629009 3.623196127 Zone D Zone E 0.463658306 0.577484374 0.993208449 0.333410759 Zone D Zone F 3.416277301 4.254958303 0.077383608 2.456601322 Zone E Zone F 2.952618995 3.677473929 0.143595411 2.123190563 20 18 16 vi 14 2 12 1- 10 8 6 4 2 0 T x L Distribution of TEMP_C T 1 Zone A Zone C Zone D B-19 Zone E Zone F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Summer Temperature Comparison by Zone Descriptive Statistics Zone A Zone C Zone D Zone E Zone F Mean 37.94084257 36.06800428 32.77845714 31.64037619 30.28288673 Standard Error 1.054099927 1.095905881 0.966990098 0.899462212 0.892690166 Median 38.92136333 35.97137464 32.94702312 31.91161626 30.41312155 Standard Deviation 1.82575463 1.898164666 1.674875979 1.55791425 1.546184723 Sample Variance 3.333379968 3.603029099 2.805209546 2.42709681 2.390687198 Skewness -1.719686391 0.228487294 -0.448309883 -0.759721896 -0.376344946 Range 3.232531049 3.792638202 3.337003853 3.080206765 3.084131234 Maximum 39.06684772 38.0126382 34.36267608 33.04485954 31.75983494 Minimum 35.83431667 34.22 31.02567222 29.96465278 28.6757037 Sum 113.8225277 108.2040128 98.33537142 94.92112858 90.84866019 Geometric Mean 37.91099478 36.03476416 32.74975984 31.61457299 30.25643621 Harmonic Mean 37.88060889 36.00161296 32.72091749 31.58855743 30.22987346 AAD 1.404350603 1.296422614 1.168523278 1.117148945 1.071455351 MAD 0.145484382 1.75137464 1.415652954 1.13324328 1.346713391 IQR 1.616265525 1.896319101 1.668501927 1.540103383 1.542065617 Multiplier 2.2 Zone A Zone C Zone D Zone E Zone F Min 35.83431667 34.22 31.02567222 29.96465278 28.6757037 Q1-Min 1.543523333 0.87568732 0.96067545 0.973481743 0.868708922 Med-Q1 1.543523333 0.87568732 0.96067545 0.973481743 0.868708922 Q3-Med 0.072742191 1.020631781 0.707826477 0.56662164 0.673356696 Max-03 0.072742191 1.020631781 0.707826477 0.56662164 0.673356696 Mean 37.94084257 36.06800428 32.77845714 31.64037619 30.28288673 Min 35.83431667 34.22 31.02567222 29.96465278 28.6757037 Q1 37.37784 35.09568732 31.98634767 30.93813452 29.54441263 Median 38.92136333 35.97137464 32.94702312 31.91161626 30.41312155 Q3 38.99410552 36.99200642 33.6548496 32.4782379 31.08647824 Max 39.06684772 38.0126382 34.36267608 33.04485954 31.75983494 Mean 37.94084257 36.06800428 32.77845714 31.64037619 30.28288673 Grand Min 0 Outliers None None None None None B-20 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station ■ Shapiro -Wilk Test Zone A Zone C Zone D Zone E Zone F W-stat 0.78368331 0.998056367 0.99240311 0.977265686 0.994679007 p-value 0.076112388 0.91577239 0.83332391 0.710929864 0.8605602 alpha 0.05 0.05 0.05 0.05 0.05 normal yes yes yes yes yes Levene's Tests type p-value means 0.986247023 medians 0.999043437 trimmed 0.986247023 ANOVA: Single Factor ' ■ DESCRIPTION Alpha 0.05 Group Count Sum Mean Variance SS Std Err Lower Upper Zone A 3 113.8225277 37.94084257 3.333379968 6.666759937 0.985203959 35.74567135 40.13601379 Zone C 3 108.2040128 36.06800428 3.603029099 7.206058198 0.985203959 33.87283306 38.2631755 Zone D 3 98.33537142 32.77845714 2.805209546 5.610419093 0.985203959 30.58328592 34.97362836 Zone E 3 94.92112858 31.64037619 2.42709681 4.854193621 0.985203959 29.44520498 33.83554741 Zone F 3 90.84866019 30.28288673 2.390687198 4.781374397 0.985203959 28.08771551 32.47805795 ANOVA Sources SS df MS F P value Eta-sq RMSSE Omega Sq Between Groups 121.0538306 4 30.26345765 10.39309731 0.001378872 0.806097795 1.86128068 0.714679127 Within Groups 29.11880525 10 2.911880525 Total 150.1726359 14 10.72661685 B-21 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station TUKEY HSD/KRAMER alpha 0.05 group mean n ss df q-crit Zone A 37.94084257 3 6.666759937 Zone C 36.06800428 3 7.206058198 Zone D 32.77845714 3 5.610419093 Zone E 31.64037619 3 4.854193621 Zone F 30.28288673 3 4.781374397 15 29.11880525 10 4.654 Q TEST group 1 group 2 mean q-stat p-value Cohen d Zone A Zone C 1.872838291 1.900965047 0.672609837 1.097522682 Zone A Zone D 5.162385432 5.239915434 0.02630016 3.025266586 Zone A Zone E 6.300466377 6.395088365 0.007610534 3.692205989 Zone A Zone F 7.657955842 7.772964948 0.00189653 4.487723405 Zone C Zone D 3.289547141 3.338950387 0.203311568 1.927743905 Zone C Zone E 4.427628086 4.494123317 0.0512 2.594683307 Zone C Zone F 5.785117551 5.8719999 0.013254244 3.390200723 Zone D Zone E 1.138080945 1.155172931 0.919492779 0.666939402 Zone D Zone F 2.49557041 2.533049514 0.42827813 1.462456819 Zone E Zone F 1.357489465 1.377876583 0.860740977 0.795517416 45 40 35 30 25 20 15 10 5 0 Distribution of TEMP_C I xT1 I Zone A Zone C Zone D B-22 Zone E Zone F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Fall Temperature Comparison by Zone Descriptive Statistics Zone A Zone C Zone D Zone E Zone F Mean 26.1994132 24.19669029 22.48399347 22.57213816 21.66615198 Standard Error 3.116457346 3.599302882 3.188780232 3.019047789 2.666924351 Median 27.01339516 22.51228754 22.20788179 23.84928669 22.59379217 Standard Deviation 5.397862463 6.234175463 5.523129377 5.22914416 4.619248476 Sample Variance 29.13691917 38.8649437 30.50495811 27.34394865 21.33745648 Skewness -0.663156006 1.12708924 0.22440128 -1.033503927 -0.867247709 Range 10.70326944 12.12221667 11.03590139 10.22166667 9.097707234 Maximum 31.14405694 31.1 28.14 27.04439722 25.7511855 Minimum 20.4407875 18.97778333 17.10409861 16.82273056 16.65347826 Sum 78.59823961 72.59007087 67.4519804 67.71641447 64.99845593 Geometric Mean 25.81174266 23.68509695 22.02810514 22.1385784 21.31884983 Harmonic Mean 25.41310691 23.20765001 21.57789358 21.68419827 20.95841072 AAD 3.839083801 4.602206473 3.770671022 3.832938401 3.341782477 MAD 4.130661783 3.534504207 5.103783178 3.195110528 3.157393322 IQR 5.351634722 6.061108333 5.517950694 5.110833333 4.548853617 Multiplier 2.2 Zone A Zone C Zone D Zone E Zone F Min 20.4407875 18.97778333 17.10409861 16.82273056 16.65347826 Q1-Min 3.286303831 1.767252103 2.551891589 3.513278069 2.970156956 Med-Q1 3.286303831 1.767252103 2.551891589 3.513278069 2.970156956 Q3-Med 2.065330892 4.29385623 2.966059105 1.597555264 1.578696661 Max-03 2.065330892 4.29385623 2.966059105 1.597555264 1.578696661 Mean 26.1994132 24.19669029 22.48399347 22.57213816 21.66615198 Min 20.4407875 18.97778333 17.10409861 16.82273056 16.65347826 Q1 23.72709133 20.74503544 19.6559902 20.33600862 19.62363522 Median 27.01339516 22.51228754 22.20788179 23.84928669 22.59379217 Q3 29.07872605 26.80614377 25.17394089 25.44684196 24.17248883 Max 31.14405694 31.1 28.14 27.04439722 25.7511855 Mean 26.1994132 24.19669029 22.48399347 22.57213816 21.66615198 Outliers None None None None None B-23 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station IShapiro -Wilk Test Zone A Zone C Zone D Zone E Zone F W-stat 0.982945178 0.945248616 0.998125608 0.955261352 0.969753318 p-value 0.749868181 0.548928975 0.917287217 0.592960471 0.666146814 alpha 0.05 0.05 0.05 0.05 0.05 normal yes yes yes yes yes Levene's Tests type p-value means 0.982326821 medians 0.997477456 trimmed 0.982326821 ANOVA: Single Factor DESCRIPTION Alpha 0.05 Group Count Sum Mean Variance SS Std Err Lower Upper Zone A 3 78.59823961 26.1994132 29.13691917 58.27383835 3.132498748 19.21977104 33.17905537 Zone C 3 72.59007087 24.19669029 38.8649437 77.7298874 3.132498748 17.21704813 31.17633246 Zone D 3 67.4519804 22.48399347 30.50495811 61.00991622 3.132498748 15.5043513 29.46363563 Zone E 3 67.71641447 22.57213816 27.34394865 54.6878973 3.132498748 15.59249599 29.55178032 Zone F 3 64.99845593 21.66615198 21.33745648 42.67491296 3.132498748 14.68650981 28.64579414 ANOVA Sources SS df MS F P value Eta-sq RMSSE Omega Sq Between Groups 38.99783629 4 9.749459072 0.331190182 0.850854906 0.116979136 0.33226003 -0.217062167 Within Groups 294.3764522 10 29.43764522 Total 333.3742885 14 23.81244918 B-24 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station TUKEY HSD/KRAMER alpha 0.05 group mean n ss df q-crit Zone A 26.1994132 3 58.27383835 Zone C 24.19669029 3 77.7298874 Zone D 22.48399347 3 61.00991622 Zone E 22.57213816 3 54.6878973 Zone F 21.66615198 3 42.67491296 15 294.3764522 10 4.654 Q TEST group 1 group 2 mean q-stat p-value Cohen d Zone A Zone C 2.002722911 0.639337178 0.990035559 0.369121492 Zone A Zone D 3.715419735 1.186088179 0.912349489 0.684788329 Zone A Zone E 3.627275045 1.1579494 0.918865113 0.668542398 Zone A Zone F 4.533261226 1.447170962 0.839238096 0.835524544 Zone C Zone D 1.712696824 0.546751 0.994482913 0.315666837 Zone C Zone E 1.624552134 0.518612221 0.995491297 0.299420906 Zone C Zone F 2.530538315 0.807833783 0.976446027 0.466403052 Zone D Zone E 0.088144691 0.028138779 0.999999957 0.016245932 Zone D Zone F 0.817841491 0.261082783 0.999691124 0.150736215 Zone E Zone F 0.905986181 0.289221562 0.999537178 0.166982147 35 30 25 20 15 10 5 0 T x 1 Distribution of TEMP_C x 1 X 1 T 1 Zone A Zone C Zone D Zone E Zone F B-25 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Spring Temperature Comparison by Zone Descriptive Statistics Zone A Zone C Zone D Zone E Zone F Mean 26.26883597 23.64887149 21.34009129 20.46571659 18.21893578 Standard Error 2.956245942 2.737096424 3.044174217 3.02320784 2.896059248 Median 27.64755093 25.51173781 22.66550432 21.73297184 19.00638889 Standard Deviation 5.120368171 4.740790072 5.272664411 5.236349581 5.01612176 Sample Variance 26.21817021 22.4750905 27.80098999 27.41935693 25.16147751 Skewness -1.123825954 -1.495222354 -1.059706383 -1.025265347 -0.689020677 Range 9.958419355 8.915691183 10.29241473 10.24009864 9.939097687 Maximum 30.55868817 27.17528392 25.82359214 24.95213829 22.79475806 Minimum 20.60026882 18.25959274 15.53117742 14.71203965 12.85566038 Sum 78.80650792 70.94661448 64.02027388 61.39714978 54.65680733 Geometric Mean 25.91521127 23.30603566 20.87030554 19.98173788 17.72595584 Harmonic Mean 25.54571118 22.94252909 20.37617021 19.47276812 17.21463042 AAD 3.77904477 3.5928525 3.87260925 3.835784628 3.575516933 MAD 2.911137246 1.663546116 3.158087824 3.219166455 3.788369176 IQR 4.979209677 4.457845591 5.146207363 5.12004932 4.969548844 Multiplier 2.2 Zone A Zone C Zone D Zone E Zone F Min 20.60026882 18.25959274 15.53117742 14.71203965 12.85566038 Q1-Min 3.523641054 3.626072533 3.567163451 3.510466093 3.075364256 Med-Q1 3.523641054 3.626072533 3.567163451 3.510466093 3.075364256 Q3-Med 1.455568623 0.831773058 1.579043912 1.609583228 1.894184588 Max-03 1.455568623 0.831773058 1.579043912 1.609583228 1.894184588 Mean 26.26883597 23.64887149 21.34009129 20.46571659 18.21893578 Min 20.60026882 18.25959274 15.53117742 14.71203965 12.85566038 Q1 24.12390987 21.88566528 19.09834087 18.22250574 15.93102463 Median 27.64755093 25.51173781 22.66550432 21.73297184 19.00638889 Q3 29.10311955 26.34351087 24.24454823 23.34255506 20.90057348 Max 30.55868817 27.17528392 25.82359214 24.95213829 22.79475806 Mean 26.26883597 23.64887149 21.34009129 20.46571659 18.21893578 Grand Min 0 Outliers None None None None None B-26 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station ■ Shapiro -Wilk Test Zone A Zone C Zone D Zone E Zone F W-stat 0.945623925 0.884196098 0.952608171 0.956072934 0.981516912 p-value 0.550506924 0.336827889 0.580872987 0.596725706 0.739542248 alpha 0.05 0.05 0.05 0.05 0.05 normal yes yes yes yes yes ■ Levene's Tests type p-value means 0.999678458 medians 0.999834831 trimmed 0.999678458 ■ ANOVA: Single Factor ' DESCRIPTION Alpha 0.05 Group Count Sum Mean Variance SS Std Err Lower Upper Zone A 3 78.80650792 26.26883597 26.21817021 52.43634041 2.933428996 19.73274886 32.80492309 Zone C 3 70.94661448 23.64887149 22.4750905 44.95018101 2.933428996 17.11278438 30.18495861 Zone D 3 64.02027388 21.34009129 27.80098999 55.60197997 2.933428996 14.80400418 27.87617841 Zone E 3 61.39714978 20.46571659 27.41935693 54.83871387 2.933428996 13.92962948 27.00180371 Zone F 3 54.65680733 18.21893578 25.16147751 50.32295502 2.933428996 11.68284866 24.75502289 ANOVA Sources SS df MS F P value Eta-sq RMSSE Omega Sq Between Groups 114.0810813 4 28.52027033 1.104793783 0.406283709 0.306479053 0.606848082 0.027185315 Within Groups 258.1501703 10 25.81501703 Total 372.2312516 14 26.58794654 B-27 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station TUKEY HSD/KRAMER alpha 0.05 group mean n ss df q-crit Zone A 26.26883597 3 52.43634041 Zone C 23.64887149 3 44.95018101 Zone D 21.34009129 3 55.60197997 Zone E 20.46571659 3 54.83871387 Zone F 18.21893578 3 50.32295502 15 258.1501703 10 4.654 Q TEST Aim group 1 group 2 mean q-stat q-stat p-value Cohen d Zone A Zone C 2.61996448 0.893140582 0.893140582 0.966322663 0.515654955 Zone A Zone D 4.928744677 1.680199072 1.680199072 0.758024411 0.970063386 Zone A Zone E 5.803119379 1.978271636 1.978271636 0.641777948 1.142155661 Zone A Zone F 8.049900195 2.744194663 2.744194663 0.357570994 1.584361527 Zone C Zone D 2.308780197 0.78705849 0.78705849 0.978560809 0.454408431 Zone C Zone E 3.183154899 1.085131054 1.085131054 0.934413774 0.626500706 Zone C Zone F 5.429935715 1.851054081 1.851054081 0.692351916 1.068706572 Zone D Zone E 0.874374702 0.298072564 0.298072564 0.999478747 0.172092275 Zone D Zone F 3.121155518 1.063995591 1.063995591 0.938569708 0.614298141 Zone E Zone F 2.246780816 0.765923027 0.765923027 0.980577815 0.442205866 35 30 25 u I 20 ~ 15 10 5 0 T X 1 Distribution of TEMP_C T 1 T x 1 T X 1 T X 1 Zone A Zone C Zone D Zone E Zone F B-28 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Appendix C Box and whisker plots depicting historical analytical data compared to 2020. 0 Historical data (2001-2019) 0 2020 data Horizontal line represents median Boxes show 25th and 75th percentiles Whiskers show 10th and 90th percentiles B C-29 D Zones E F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Total phosphorus (mg/L) 0.14 0.12 - 0.10 - 0.08 - 0.06 - 0.04 - 0.02 - 0.00 0.07 0 0 O 0 0 o O 8 I 0 I B D E F 0.06 - - 0.05 - 0) E a? 0.04 - co 0. co 0 0.03 - 0. 0 0 0.02 - 0.01 - 0.00 O 0 0 0 0 O 0 cU 0 8 B C-30 D E F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Ammonia (mg/L) Nitrite -nitrate (mg/L) 0.20 0.15 - 0.10 - 0.05 - 0.00 HHTTTT LT, B C-31 D D E E F F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Total Kjeldahl nitrogen (mg/L) Total organic carbon (mg/L) 25 B D E F 20 - 15 - 10 - 5- 0 B D C-32 E F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Calcium (mg/L) Magnesium (mg/L) 10 8- 6 No Data I 1 8 I 0 4- 2- 0 B B D D C-33 E E F F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Chloride (mg/L) Sulfate (mg/L) 10 8- 6 4- 0 0 1 e e e 0 0 0 0 2- 0 12 B D E F 10 - 8 6- 8 o 1 0 0 0 4- 2- 0 B D C-34 E F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Turbidity (NTU) Total dissolved solids (mg/L) B B C-35 D D E E F F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Alkalinity (mg/L) Total hardness (mg/L) 35 30 - 25 - 20 - 15 - I O o O O O o o O O 10 - 5- 0 B B D D C-36 E E F F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Arsenic (µg/L) B B C-37 D D E E F F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Mercury (ng/L) Selenium (µg/L) 2.5 F 2.0 - 1.5 - No Data 1.0 - 0 0 0.5 - 0.0 C-38 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station B D C-39 E F CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Appendix D RDL and LOQ values for Nutrient Parameters collected in 2020 at Belews Lake Nutrient Parameter Duke Energy Analytical Lab Pace Analytical Laboratory RDL (mg/L) LOQ (mg/L) Ammonia 0.02 0.10 Nitrate -Nitrite 0.01 0.020 Orthophosphate 0.005 0.050 Total Kjeldahl Nitrogen 0.100 0.050 Total Phosphorous 0.005 0.050 D-40 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Appendix E Tables of fish caught seasonally from Belews Lake during 2020 using electrofishing and gill nets. Table E-1. Number and biomass of fish collected from electrofishing within six zones of Belews Lake during winter 2020. Species A Origin No. Kg B No. Kg No. C Kg D No. Kg E No. Kg No. Kg Centrarchidae Alabama Bass Black Crappie Bluegill Green Sunfish Hybrid sunfish Largemouth Bass Pumpkinseed Redbreast Sunfish Redear Sunfish Warmouth White Crappie Clupeidae Alewife Gizzard Shad Threadfin Shad Cyprinidae Common Carp lctaluridae Blue Catfish Channel Catfish Moronidae White Perch Percidae Yellow Perch Poeciliidae Eastern Mosquitofish Total Number of taxa Introduced 29 Native Native 546 Introduced 1 Hybrid 14 Native 16 Native Native Introduced 36 Native Introduced Introduced Native Introduced Introduced 10.29 49 1 1.69 564 0.01 11 0.64 21 7.23 11 2 2.74 58 3 Introduced 1 9.00 Introduced Introduced Native Native 1 <0.01 644 31.61 8 8.49 26 8.66 22 1.66 36 1.49 16 4.98 0.37 9 1.30 2.29 28 0.50 186 0.56 122 0.70 187 3.20 0.05 4 0.20 1 0.01 1 <0.01 9 0.10 0.39 13 0.71 8 0.28 4 0.24 7.09 1 0.19 2 2.01 6 7.01 18 5.60 1 0.03 <0.01 4 0.03 6 0.09 4 0.05 2 0.06 2.33 19 2.20 3 0.27 4 0.23 36 4.71 0.08 3 0.04 2 0.03 5 0.74 12 5.46 1 0.02 10 3.32 1 <0.01 171 0.51 1 0.01 1 2.47 4 0.60 6 0.60 732 26.21 95 12.31 223 4.63 185 9.81 480 27.97 10 7 7 10 16 E-41 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table E-2. Number of and biomass fish collected from electrofishing within six zones of Belews Lake during spring 2020. Species A Origin No. Kg B No. Kg C No. Kg D No. Kg E No. Kg F No. Kg Centrarchidae Alabama Bass Black Crappie Bluegill Green Sunfish Hybrid sunfish Largemouth Bass Redbreast Sunfish Redear Sunfish Warmouth White Crappie Clupeidae Gizzard Shad Cyprinidae Common Carp Grass Carp Satinfin Shiner Ictaluridae Channel Catfish Moronidae White Perch Percidae Yellow Perch Total Number of taxa Introduced 56 Native Native 483 Introduced 2 Hybrid 4 Native 10 Native 1 Introduced 27 Native Introduced Native 24.69 61 13.83 60 20.38 79 25.18 49 13.00 15 9 1.72 368 3.86 97 2.25 44 0.64 83 1.32 297 0.02 15 0.15 2 0.09 5 0.17 4 0.09 9 0.08 15 0.83 5 0.36 8 0.94 2 6.13 22 11.91 12 10.82 8 6.37 20 16.09 46 0.06 25 1.03 22 0.96 37 1.49 16 3.13 54 1.88 43 2.38 16 1.73 13 1.49 7 6 0.15 1 9 4 1.14 Introduced 1 4.62 1 2.95 Introduced 1 6.08 Native Introduced Introduced Native 1 1.78 1 0.11 5.14 18.67 4.76 0.06 0.28 20.91 0.65 1.17 0.02 2.66 13 4.56 3 15.88 1 0.01 2 2.31 9 6.10 1 0.19 3 0.07 585 46.54 542 37.84 254 37.78 174 35.24 217 36.81 436 81.13 11 9 7 6 8 16 Table E-3. Number of and biomass fish collected from electrofishing within six zones of Belews Lake during summer 2020. Species A Origin No. Kg B No. Kg c No. Kg D No. Kg E No. Kg F No. Kg Centrarchidae Alabama Bass Bluegill Green Sunfish Hybrid sunfish Largemouth Bass Redbreast Sunfish Redear Sunfish White Crappie Clupeidae Alewife Gizzard Shad Threadfin Shad Cyprinidae Satinfin Shiner Ictaluridae Channel Catfish Total Number of taxa Introduced Native Introduced Hybrid Native Native Introduced Introduced Introduced Native Introduced Native 2 0.17 22 3.67 27 1.88 36 340 1.15 348 2.54 149 2.28 302 6 0.06 11 0.07 3 0.03 5 16 0.45 12 0.36 3 0.28 6 4 0.01 69 2.77 42 1 <0.01 5 0.14 41 1.27 132 1 0.02 10 0.64 10 0.44 9 Introduced 370 7 1.85 477 10.18 7 3.69 16 0.25 42 9.32 2.18 118 1.82 434 6.88 0.03 1 0.01 17 0.20 0.38 3 0.18 3 0.10 0.99 4 0.17 57 8.08 2.09 26 0.98 19 0.43 0.72 4 0.50 14 2.19 1 0.34 2 <0.01 1 0.38 6 1.46 43 0.12 1 0.82 2 2.08 234 7.00 536 12.55 7 10 5 0.02 3 1.93 8 4.27 177 5.83 649 33.39 9 12 E-42 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table E-4. Number of and biomass fish collected from electrofishing within six zones of Belews Lake during fall 2020. Species A B C D E F Origin No. Kg No. Kg No. Kg No. Kg No. Kg No. Kg Centrarchidae Alabama Bass Introduced 25 5.99 45 7.40 77 6.34 52 10.12 49 5.26 46 12.97 Black Crappie Native 1 0.28 Bluegill Native 408 0.97 506 2.25 146 1.94 176 1.84 117 2.70 229 5.35 Green Sunfish Introduced 4 0.02 15 0.06 11 0.11 1 <0.01 2 0.08 25 0.63 Hybrid sunfish Hybrid 14 0.53 16 0.10 22 0.62 10 0.26 4 0.29 5 0.26 Largemouth Bass Native 6 1.71 16 1.93 6 3.19 2 0.22 9 5.14 25 5.57 Pumpkinseed Native 1 0.03 Redbreast Sunfish Native 2 <0.01 5 0.10 37 0.90 61 0.87 29 1.23 21 0.43 Redear Sunfish Introduced 11 0.14 29 1.28 13 1.78 7 0.40 2 0.44 18 3.30 Warmouth Native 6 0.15 1 <0.01 2 0.10 Clupeidae Gizzard Shad Native 3 1.09 1 0.42 2 0.95 2 0.35 Cyprinidae Common Carp Introduced 1 2.30 Golden Shiner Native 2 <0.01 Satinfin Shiner Native 10 0.02 lctaluridae Channel Catfish Introduced 2 1.58 1 0.02 1 0.03 9 4.75 Flathead Catfish Introduced 1 1.01 Percidae Yellow Perch Native 7 0.17 Poeciliidae Eastern Mosquitofish Native 1 <0.01 Total 475 13.25 636 14.21 314 15.30 312 14.68 214 15.13 402 35.11 Number of taxa 10 9 9 9 8 15 E-43 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table E-5. Number of and biomass fish collected from gill netting within six zones of Belews Lake during winter 2020. Species A Origin No. Kg B No. Kg c No. Kg D No. Kg E No. Kg No. Kg Catostomidae Quillback Centrarchidae Alabama Bass Black Crappie Bluegill Hybrid sunfish Largemouth Bass Redbreast Sunfish Redear Sunfish Clupeidae Alewife Gizzard Shad Threadfin Shad Cyprinidae Common Carp Ictaluridae Blue Catfish Channel Catfish Flathead Catfish Moronidae White Perch Total Number of taxa Native 1 1.05 Introduced Native Native Hybrid Native Native Introduced Introduced Native Introduced Native 23 12.19 30 14.59 31 17.54 14 7.05 31 19.82 3 0.92 2 0.49 9 2.98 1 <0.01 10 0.08 1 0.01 1 0.01 1 0.11 6 1.31 7 3.42 1 0.43 3 2.73 4 2.47 2 0.10 2 0.06 3 0.30 2 0.10 1 0.06 2 0.40 1 0.01 12 4.81 32 12.25 13 6.01 9 4.89 2 1.12 1 0.02 Introduced 1 Introduced 10 Introduced Introduced 1 58 9 7.40 1 9.60 2.22 7 2.15 3 0.94 2 1.54 1 3.04 2 3.71 <0.01 3 0.21 29.89 96 36.55 64 37.72 32 16.44 42 27.52 10 10 7 6 11 3.78 23 3.89 3 4.38 4 0.05 10 3.52 21 0.15 1 2.82 9 2.05 11 1.71 93 22.35 9 Table E-6. Number of fish and biomass collected from gill netting within six zones of Belews Lake during spring 2020. Species A Origin No. Kg B No. Kg No. c Kg D No. Kg E No. Kg No. Kg Centrarchidae Alabama Bass Black Crappie Bluegill Green Sunfish Largemouth Bass Redear Sunfish White Crappie Clupeidae Alewife Gizzard Shad Threadfin Shad Cyprinidae Common Carp Ictaluridae Blue Catfish Channel Catfish Flathead Catfish Moronidae White Perch Total Number of taxa Introduced 4 Native Native Introduced Native Introduced Introduced Introduced Native 4 Introduced Introduced Introduced Introduced Introduced 1.54 9 4.40 16 5.70 9 4.19 11 5.08 8 1 0.37 11 2 0.01 1 1 2 0.62 1 0.27 1 0.70 4 3.76 5 2 0.28 1 0.14 1 0.05 2 0.67 1 1 1 1.38 38 0.01 10 0.10 1 10.39 18 8.46 5 2.42 8 3.85 75 2 1 3.64 9 5.74 17 4.79 4 4.79 3 2.95 1 0.82 1 1.98 1 <0.01 Introduced 3 0.23 2 0.17 1 0.18 4 0.88 21 9.70 73 23.00 42 23.17 26 11.20 5 9 7 8 4 12.62 4.48 2.60 0.01 0.04 2.01 0.14 0.17 0.01 17.62 0.02 1 1.79 12 8.94 1 4.57 1 1.13 11 2.49 13 2.95 52 34.93 132 40.11 9 13 E-44 CWA §316(a) Balanced and Indigenous Community Study Report Belews Creek Steam Station Table E-7. Number of fish and biomass collected from gill netting within six zones of Belews Lake during summer 2020. Species A B C D E F Origin No. Kg No. Kg No. Kg No. Kg No. Kg No. Kg Centrarchidae Alabama Bass Introduced 4 0.59 25 3.71 5 1.70 17 5.73 3 0.71 5 1.39 Black Crappie Native 6 0.91 Bluegill Native 1 0.22 Largemouth Bass Native 1 0.39 1 0.10 1 0.48 1 0.08 Redbreast Sunfish Native 1 <0.01 Redear Sunfish Native 9 1.32 5 1.02 6 1.97 6 1.45 1 0.09 White Crappie Introduced 1 0.41 Clupeidae Gizzard Shad Native 1 0.39 32 10.38 6 1.87 14 5.97 2 0.44 18 2.87 Threadfin Shad Introduced 1 0.01 Cyprinidae Common Carp Introduced 3 9.25 lctaluridae Channel Catfish Introduced 19 4.61 41 14.28 3 1.65 1 0.08 8 5.31 18 11.58 Flathead Catfish Introduced 1 1.24 2 2.13 1 6.00 Moronidae White Perch Introduced 4 0.37 7 0.75 Total 26 6.18 115 39.37 21 7.58 42 16.07 20 7.99 57 24.00 Number of taxa 5 7 6 7 5 8 Table E-8. Number of fish and biomass collected from gill netting within six zones of Belews Lake during fall 2020. Species A B C D E F Origin No. Kg No. Kg No. Kg No. Kg No. Kg No. Kg Centrarchidae Alabama Bass Introduced 23 5.58 21 3.80 26 6.93 25 7.53 21 6.88 16 3.36 Black Crappie Native 2 0.78 5 1.67 4 0.93 1 0.54 7 1.30 Bluegill Native 2 0.12 3 0.02 1 0.01 Green Sunfish Introduced 1 0.01 1 0.01 Largemouth Bass Native 3 1.65 1 1.05 6 5.59 7 2.79 Redear Sunfish Introduced 4 0.48 5 0.53 4 0.68 1 0.15 1 0.18 Warmouth Native 4 0.28 Clupeidae Gizzard Shad Native 16 4.72 28 9.63 40 14.77 14 6.31 10 4.48 10 2.87 Threadfin Shad Introduced 2 0.02 Cyprinidae Common Carp Introduced 1 1.69 lctaluridae Channel Catfish Introduced 22 6.23 14 5.53 4 2.47 4 2.32 5 3.74 11 4.27 Flathead Catfish Introduced 1 0.91 2 2.75 Moronidae White Perch Introduced 4 0.38 3 0.41 1 0.10 6 0.96 Total 72 18.17 88 26.22 85 29.70 45 16.32 43 21.22 60 15.75 Number of taxa 11 9 9 5 5 8 E-45