NC0000272_2013 Dioxin in Fish Tissue Report_20140331

75 evergreen,,, 1 a Office
packaging 75 Main Street• Conlon, NC 28716
NMM#08-14
January 31, 2014 CERTIFIED MAIL
RETURN RECEIPT REQUESTED
Landon Davidson 7099 3220 0371 4392
Regional Supervisor
Division of Water Resources
Asheville Regional Office
North Carolina Department of Environment
and Natural Resources
2090 US Highway 70
Swannanoa,North Carolina 28778
Subject: 2013 Dioxin in Fish Tissue Report
NPDES NC 0000272
Blue Ridge Paper Products Inc dba Evergreen Packaging
Canton Mill
Dear Mr.Davidson—
Enclosed is the subject report with results of August 2013 dioxin in fish tissue for Waterville Lake as
required by special condition I.A.(9.)of the May 2010 NPDES permit.
In January 2007,the NC Department of Health and Human Services(DHHS) lifted the fish advisory
for common carp in Waterville Lake and requested two additional years of fish tissue sampling and
surveillance,which were completed in 2009. The May 2010 NPDES Permit for the Blue Ridge
Canton Mill requires three(3)additional years of fish tissue sampling and surveillance in the
summer of 2011,2013 and 2014. This report completes the 2013 surveillance. The concentrations
of dioxin and furan congeners in channel catfish and common carp fillet samples collected from
Waterville Lake in August 2013 remain below the 4 ppt TEQ fish advisory level established by the NC
DHHS.
Very truly y
�ick McCracken
EHS&S Water Supervisor
828-646-2874 RECEIVED
nick.mccracken(a)eveMack.com Division of water Resources
Enclosure—2013 Dioxin in Fish Tissue Report � _3
MU
Water Ouatity Regtonat Ooeratlona
Asheville Regional Office
1i h [)y desiaf)

2013 Dioxin in Fish Tissue Report
31 Jan 2014, Page 2
cc w/enclosure:
NC DENR (2 copies)
Division of Water Resources
Water Resources Section
ATTENTION—CENTRAL FILES
1617 Mail Service Center
Raleigh,North Carolina 27699-1617
Sergei Chernikov, NC DWR—Complex Permits Section(electronic)
Rick Smith,Duke Power(electronic)
Larry Wilson, UfK(electronic)
C-File(cover letter only)

RESULTS OF 2013
DIOXIN MONITORING IN FISH TISSUE
Prepared for:
Blue Ridge Paper Products Inc., dba Evergreen Packaging
Canton Mill
Canton,North Carolina
Prepared by:
Theodore B. Henry,Ph.D.
Center for Environmental Biotechnology and
Departments of Forestry,Wildlife and Fisheries
University of Tennessee
Knoxville,Tennessee
J. Larry Wilson,Ph.D.
Departments of Forestry,Wildlife and Fisheries
University of Tennessee
Knoxville,Tennessee
January 2014

TABLE OF CONTENTS
Page
List of Tables . . . . . . . . . . . . . . . . .
List of Figures. . . . . . . . . . . . . . . . .
EXECUTIVE SUMMARY . . . . . . . . . . . . . iv
1. INTRODUCTION . . . . . . . . . . . . . 1
2. SAMPLING LOCATIONS . . . . . . . . . . . . . 2
3. SAMPLING OBJECTIVES . . . . . . . . . . . . . 6
4. FISH COLLECTION TECHNIQUES AND LEVEL OF EFFORT . . . 7
5. SAMPLE PREPARATION . . . . . . . . . . . . . 13
6. ANALYTICAL RESULTS . . . . . . . . . . . . . 15
7. REFERENCES . . . . . . . . . . . . . . . 27
8. APPENDICES . . . . . . . . . . . . . . 30
APPENDIX A: CHAIN OF CUSTODY FORM
APPENDIX B: TESTAMERICA WEST SACRAMENTO
ANALYTICAL REPORT
APPENDIX C: BLUE RIDGE PAPER CANTON MILL FISH FILLET
TISSUE ANALYSIS RESULTS 1990-2004
APPENDIXD: 2006 REVISIONS TO DIOXIN MONITORING PLAN
i

LIST OF TABLES
Number Title Page
2-1 Pigeon River sampling station information . . . . . . . . . 3
4-1 Fish collection techniques and level of effort . . . . . . . . 8
4-2 Summary of fish composites collected at two stations in Waterville Lake,
August 2013 . . . . . . . . . . . . . . . . . . 9
6-1 Summary of Pigeon River fish tissue analysis results—2013 . . . . 17
6-2 Toxicity equivalence factors for CDD/F congeners . . . . . . . 18
6-3 Summary of CDD/F isomer analyses,toxicity equivalent
factors, and toxicity equivalent values for the 2013 Waterville Lake fish
tissue composites . . . . . . . . . . . . . . . . 20
6-4 Blue Ridge Paper Canton Mill fish fillet tissue analysis results
2005—2013 . . . . . . . . . . . . . . . . 22
�fv
n

I
LIST OF FIGURES
Number Title Page
ES-1 TCDD Concentrations in Common Carp Fillets Collected from the Pigeon River,
1990-2013(Stations 4A and 413) . . . . . . . . . . . . . . . v
ES-2 TCDD Concentrations in Channel Catfish Fillets Collected from the Pigeon River,
1997-2013 (Stations 4A and 413) . . . . . . . . . . . . . . . vi
2-1 Historical sampling station locations on the Pigeon River . . . . . . . . 4
2-2 Sampling Stations 4A and 4B on the Pigeon River . . . . . . . . . . 5
4-1 a Lengths of fish collected from Waterville Lake for tissue analyses,
2004-2013 . . . . . . . . . . . . . . . . . . 11
4-lb Weights of fish collected from Waterville Lake for tissue
analyses,2004-2013 . . . . . . . . . . . . . . . . . . . 12
6-1 TCDD Concentrations in Common Carp Fillets Collected from the Pigeon River,
1990-2013 (Stations 4A and 413) . . . . . . . . . . . . . . 25
6-2 TCDD Concentrations in Channel Catfish Fillets Collected from the Pigeon River,
1997-2013 (Stations 4A and 413) . . . . . . . . . . . . . . . 26
iii

EXECUTIVE SUMMARY
The Blue Ridge Paper Products Inc., dba Evergreen Packaging, Canton Mill contracted the
Department of Forestry, Wildlife and Fisheries of the University of Tennessee, Knoxville(UTK)
to collect fish tissue samples for dioxin analysis from the Pigeon River during the summer of
2013 in accordance with NPDES permit conditions. This was the second sampling effort
conducted by the university for the current permit. UTK field personnel followed the March
2006 revision (Wilson 2006) to the December 2001 Fish Tissue Sampling Plan prepared by EA
Engineering, Science and Technology, Inc., (EA Engineering 2001a) of Chicago, Illinois. UTK
prepared this 2013 fish tissue sampling report using the revised protocol of 2006.
Bottom feeding species (channel catfish and common carp) were collected in August 2013 from
two locations (Stations 4A and 413) in the Pigeon River (Waterville Lake) and analyzed for
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8-tetrachlorodibenzofilran(TCDF), and other
CDD and CDF congeners.
Concentrations of 2,3,7,8-TCDD and 2,3,7,8-TCDF in channel catfish fillets were below
detection level (DL) in Stations 4A and 4B (4A DL = 0.48 ppt for both 2,3,7,8-TCDD and
2,3,7,8-TCDF; 4B DL = 49 ppt for both 2,3,7,8-TCDD and 2,3,7,8-TCDF)... In common carp,
2,3,7,8-TCDD was 1.8 ppt in fillet composites collected at Station 4A and was 0.70 ppt
(estimated) at Station 4B. For 2,3,7,8-TCDF the concentrations in common carp fillets were 2.0
ppt at Station 4A and 1.4 ppt at Station 4B. The concentration of 2,3,7,8-TCDF in the common
carp whole body sample collected at Station 4B was 2.4 ppt and 5.8 ppt for 2,3,7,8-TCDD and
2,3,7,8-TCDF respectively(Table 6-1).
The mean weights of common carp collected from Stations 4A and 4B (5.052 kg, Station 4A;
4.364 kg, Station B) were lower than those collected in 2007 (6.490 kg, Station 4A; 6.552 kg,
Station 4B),but consistent with weights of common carp collected in other previous years (2011,
Station A, 4.144, Station B, 5.542; 2009, 4.303 kg, Station 4A; 4.848 kg, Station B; 2008,4.727,
Station 4A, 4.067, Station 413; 2006, 4.505 kg, Station 4A, 5.637 kg, Station 413; 2005, 3.857 kg
- Station 4A, 4.695 kg - Station 4B; 2004, 3.750 kg - Station 4A, 3.900 kg - Station 4B; 1999-
2003, 3.193 kg, Station 4A, 5.042 kg, Station 4B). Lipid concentrations in common carp fillets
in 2013 were 4.2-8.7%.
In January 2007,the NC DHHS lifted the fish advisory for common carp in Waterville Lake and
requested two additional years of fish tissue sampling and surveillance, which was completed in
2009. The May 2010 NPDES Permit for the Blue Ridge Canton Mill requires three(3),
additional years of fish tissue sampling and surveillance. All concentrations in fish tissue fillets
remain below the NC DHHS 4 ppt TEQ action level for fish consumption advisory.
i�
iv

Figure ES-1. TCDD Concentrations in Common Carp Fillets Collected from the Pigeon River,
1990-2013
(Stations 4A and 413)
70
60 ---
+Station 4A
50 i
12 -,t- Station 48
o
M 40
30
O
� 20 �
C1 �
10
A b
0 --
1990 1991 1992 1993 2994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013
TIME
b)TCDD concentrations at Station 4B were not detected, therefore,the values plotted represent the detection limit for those samples.
The detection limit for 2,3,7,8-TCDD was elevated in the 2007 carp fillet for Station 4B because of a I0-fold dilution of the sample
which was required to overcome sample matrix effects(high lipid concentration) during analysis. Consequently, reporting the
detection limit for this sample can give a misleading impression that the concentration of 2,3,7,8-TCDD increased that year.
v

r
Figure ES-2. TCDD Concentrations in Channel Catfish Fillets Collected from the Pigeon River,
1997-2013
(Stations 4A and 4B)
2.50
f Station 4A
2.00
--l- Station 4B
0
- c
�A
ro 1.50
a ; %
i %
A �
a : `
1.00 `
G a b
— h ; �`
FAr----,/-, b `.
- a,
b ,�A,_- a b a b
A----A''' a a a
0.00 1 1 1
1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013
TIME
a) TCDD concentrations were not detected at Station 4A, therefore the values plotted represent the detection limit for the sample.
b) TCDD concentrations were not detected at Station 413, therefore the values plotted represent the detection limit for the sample.
c)The 2011 TCDD result for catfish fillets in 2011 at Station 4B was above detection limit at 1.7 ppt . The TEQ for the 2011 Station
4B catfish fillets remained below the 4 ppt action level of the NC DHHS.
vi

�. 1.INTRODUCTION
The Blue Ridge Paper Products hic., dba Evergreen Packaging, Canton Mill contracted the
Department of Forestry, Wildlife and Fisheries of the University of Tennessee,Knoxville(UTK)
to collect fish tissue samples for dioxin analysis from the Pigeon River during the summer of
2013 in accordance with NPDES permit conditions. UTK field personnel followed the March
2006 revision (Wilson 2006) to the December 2001 Fish Tissue Sampling Plan prepared by EA
Engineering, Science and Technology, Inc. (EA Engineering 2001 a) of Chicago, Illinois.
This report details the results of a study conducted during August 2013 to determine the
concentrations of 2,3,7,8-TCDD (dioxin) and 2,3,7,8-TCDF (firan) in bottom-feeding fish
collected from the Pigeon River near Canton, North Carolina. The report follows the template
developed by EA Engineering for prior reporting years. The 2013 fish tissue study is number 22
in a series of fish tissue surveys designed and conducted to be responsive to the requirements of
A.(9.) Dioxin Monitoring Special Condition in Blue Ridge Paper's current NPDES permit for the
Canton Mill (Permit No.NC0000272). Sampling locations, selection of target species, sampling
methods, and sample preparation/preservation techniques are in accordance with the study plan.
The approved study methods and scope detailed herein generally follow those used since 1990
(EA 1990, 1991, 1992, 1993a, 1993b, 1994, 1995, 1996, 1997, 1998, 2000, 2001b, 2001c, 2003,
2004, Wilson 2006, Henry and Wilson 2007, 2008, 2009, 2011). However, selected project
details were modified to be responsive to the suggestions/recommendations of the North
Carolina Department of Environment and Natural Resources (NCDENR), the Tennessee
Wildlife Resources Agency, U.S. EPA Region IV, and the North Carolina Department of Health
and Human Services (NC DHHS). The principal change in the program was that the collection
and analysis of sportfish composite samples have not been required since 2000. In 2006,project
details were modified again to be responsive to the North Carolina Division of Water Quality
(NC DWQ) and these modifications were adhered to in the present investigation(i.e., 2013).
The principal changes to the program in 2006 were the elimination of sampling of the main stem
of the Pigeon River beginning in 2006 and the collection of at least one whole body bottom
feeder sample from Waterville Lake (Stations 4A or 4B). These changes are included*in more
detail in Appendix D.
The 2013 study was conducted during August, during which time biologists from UTK collected
and prepared fish tissue samples from the two Waterville Lake sampling locations (Stations 4A
and 4B) on the Pigeon River. Details relevant to the location of Pigeon River sampling stations
and fish tissue sampling objectives follow in Sections 2 and 3, respectively. Fish collection
techniques and level of effort are detailed in Section 4; methods of sample preparation and
shipment are presented in Section 5. Analytical results are summarized in Section 6 and
references are provided in Section 7.
1

2. SAMPLING LOCATIONS
In accordance with the study plan (Wilson 2006) that was implemented in 2006, 2007, and 2008
(Henry and Wilson 2007, 2008, 2009, 2011), fish were collected from two sampling locations on
the Pigeon River. For this sampling period two sites (Stations 4A and 4B) were sampled.
Detailed sampling station information for both sites is provided in Table 2-1 and Figure 2-2.
Monitoring Stations 4A and 4B are located in Waterville Lake at RM 41.5 and 39.0, respectively
(Figure 2-1). Station 4A is located approximately 21.8 RM downstream from the Mill outfall,
near the Messer Branch-Pigeon River confluence (Figure 2-2). Monitoring Station 4B is in the
vicinity of the Wilkins Creek-Pigeon River confluence, approximately 24.3 RM downstream
from the Canton Mill outfall (Figure 2-2). Appropriate habitats were sampled within each study
reach in an effort to collect the desired complement of fishes.
Fish sampling was conducted in the vicinity of each location described above; however, the
distance or effort at each station depended on how difficult it was to collect fish at that station.
The Station 4B study area consisted of an approximate 1.0 RM reach of Waterville Lake located
from the dam to the confluence of Wilkins Creek. Sampling at Stations 4A and 413 included
electrofishing and setting of trot lines near the shore along both the left and right banks of
Waterville Lake.
Reservoir water level in 2013 was approximately at full pool and was similar to water levels in
2006. Stations 4A and 4B sample areas covered approximately the.same surface area as water
/ levels in 2004-2006.
�LJ
2

l
Table 2-1. Pigeon River sampling station information.
Station Station Location and Site Description/Habitat Type Fish Community
Number RM Distance from Outfall
4A 41.5 Upper Waterville Lake Characterized by deep-water lentic There are no dominant species. Bluegill,black
(21.8 RM downstream from habitat with bedrock covered by crappie,flathead and channel catfish,small mouth
the Canton Mill outfall) loose, unconsolidated bottom bass, and largemouth bass are common. Common
sediments. Maximum depth carp are present but not common.
sampled approximately 3 in.
4B 39.0 Lower Waterville Lake Characterized by deep-water lentic There are no dominant species. Bluegill,black
from the dam to confluence habitat,bedrock, and soft crappie,flathead and channel catfish,small mouth
with Wilkins Creek(24.3 sediments. Maximum depth bass, and largemouth bass are common. Common
RM downstream from the sampled approximately 12 in. Very carp are present but not common.
Canton Mill outfall) I steep banks with little cover.
3

Figure 2-1. Historical sampling station locations on the Pigeon River; fish tissues were
collected from two sites(4A and 413) for dioxin analyses in 2013. More precise
geographical locations of each site are presented in Figure 2-2.
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Figure 2-2. Sampling Stations 4A (indicated by A)and 4B (indicated by B)on the Pigeon River.
5

3. SAMPLING OBJECTIVES
In accordance with the study plan (Wilson 2006), the goal of the fish collection effort was to
collect one composite bottom feeder fillet sample at both sampling stations 4A and 4B. Each
composite consisted of 3 to 5 similarly-sized (shortest specimen within 75% of the length of the
longest) adult individuals of the target species. Common carp (Cyprinus carpio) and channel
catfish (Ictalurus punctatus) were the targeted species at sites 4A and 4B. In addition to fillet
samples, one whole body common carp was collected from Station 4B.
In summary fish were collected as follows:
• Bottom feeder fillet composite—one sample at both locations
• Catfish fillet composite—one sample at both locations
• Common carp whole body composite—one sample from Station 4B
Every reasonable effort was made to collect the desired size, species and/or number of fish,
however, the outcome of the sampling effort each year is dependent on physical river conditions
and the natural diversity and abundance of target fishes at each sampling location. The 2013
Pigeon River collections yielded the desired species at each location. In addition, the number of
specimens collected made it possible to composite individuals of similar weight and length (with
larger/adult specimens preferred), and the 75%rule was met for all samples.
u 6

4. FISH COLLECTION TECHNIQUES AND LEVEL OF EFFORT
Sampling was conducted during 5-6 August 2013. Notes were recorded at each sampling station
with regard to the type of sampling gear, level of effort(time), and habitat(Tables 2-1 and 4-1).
All fish submitted for tissue analysis (including whole body specimen) were measured to the
nearest millimeter (total length) and weighed to the nearest gram (wet weight). These data are
summarized in Table 4-2. For 2013, the mean total length of carp was 683 min and fish ranged
from 630-722 mm. In 2011, the mean total length for carp was 685 min and fish ranged from
625-740 mm. Previously, fish collections were conducted by EA Engineering, Science, and
Technology(see References), and the mean for carp total length was 618 mm for fish submitted
for analysis between 1993 and 2003.
The field investigators were equipped with an array of collecting gear, which enabled sampling
of all habitats regardless of river conditions. U.S. EPA recommends active methods of fish
collection in the Sampling Guidance Manual (Versar 1984), such as electrofishing, trawling,
angling, or seining. These are preferred over passive methods (e.g., gill nets,trap nets, trot lines)
because samples are collected from more definable areas (Versar 1984). A boat electrofishing
unit(pulsed direct current) was used at both stations. The boat electrofisher was equipped with a
Smith Root Type VIA electrofisher, and powered by a 240-volt, 5000-watt generator.
Electrofishing techniques followed those described in the National Dioxin Study(Versar 1984).
{_ Fish collection techniques and level of effort (time) expended at both stations are summarized in
Table 4-1. Total study effort for collection of fish at Stations 4A/413 was 5.8 hours electrofishing
and trot lines were used for 50.1 hours.
7

Table 4-1. Fish collection.techni ues and level of effort.
Station Sampling Sampling
Number RM Dates Techniques Level of Effort Comments
4A 41.5 5-6 Aug 2013 Boat An approximate 1.0 RM reach of river was sampled and the entire
electrofishing 2.4 hours area was electrofished. No fish captured by trot lines.
Trot lines(two) 5.3 hours total
4B 39.0 5-6 Aug 2013 Boat 3.4 hours An approximate 1.4 RM reach was sampled by electrofishing. Trot
electrofishing lines were set throughout the area and yielded two channel catfish.
Trot lines(two) 44.8 hours total
8

Table 4-2. Summary of fish composites collected at two stations in Waterville Lake,August
2013. A composite sample was generated for each site and each composite consisted of the
right-side fillet(R) from all five fish collected from the site. A whole body(WB)composite
from a single common carp was obtained from site 4B.
Total Total
Length whole body
Date Station Species (mm) (g) Sample type Composite
5 Aug 13 4A Channel catfish 511 1270 Fillet R
(RM 41.5)Channel catfish 489 925 Fillet R
Channel catfish 460 935 Fillet R
Channel catfish 485 1090 Fillet R
Channel catfish 391 550 Fillet R
MEAN 467 954
4A Common carp 690 4555 Fillet R
(RM 41.5)Common carp 631 3525 Fillet R
Common carp 722 5980 Fillet R
Common carp 719 6300 Fillet R
Common carp 680 4900 Fillet R
MEAN 688 5052
5 Aug 13 4B Channel catfish 498 1140 Fillet R
(RM 39.0)Channel catfish 445 920 Fillet R
6 Aug 13 Channel catfish 537 1200 Fillet R
Channel catfish 525 1355 Fillet R
Channel catfish 500 1050 Fillet R
MEAN 501 1133
5 Aug 13 4B Common carp 685 4560 Fillet R
6 Aug 13 (RM 39.0)Common carp 695 4960 Fillet R
Common carp 697 4700 Fillet R
Common carp 677 4480 Fillet R
Common carp 630 3120 Fillet R
MEAN 677 4364
6 Aug 13 4B Common carp 724 5080 Whole body WB
(RM 39.0)
9

t Target species were collected at both sampling stations in 2013. Common carp, the target
bottom feeder at Stations 4A, and 4B, were collected (and prepared for fillet and/or whole body
analysis) at those stations. Because of the physiography of the Waterville Lake Stations 4A and
4B,trot-lines were used in addition to the electrofishing gear for the collection of bottom feeding
species. All trot lines were pulled and examined on a regular basis to reduce stress or specimen
mortality. All specimens submitted for analysis appeared healthy and in good condition: Lengths
and weights for each fish making up each composite are provided in Table 4-2.
Bottom feeder fillet composites samples consisted of five channel catfish and five common carp
at Station 4A, and five channel catfish and five common carp at Station 4B. A single bottom
feeder whole body common carp from Station 4B was submitted for analysis (Table 4-2).
All 20 composite fillets submitted for analysis in 2013 met the US EPA Region IV
recommendation (Cunningham 1990) that the smallest specimen in each composite be equal to
or greater than 75% of the total length of the largest specimen in that composite (Tables 4-2 and
6-4).
Figure 4-la-b illustrates the length and weight distribution of fish collected for,tissue analysis.
from Waterville Lake since 2004. The size distribution of catfish remained similar between
2004 and 2013. The size of the carp taken for whole body tissue analysis from Station 4B in
2013 was similar to size of whole body' fish collected in previous years.
10

Max, Min and Mean of Lengths taken from
80o
Fish Tissue Samples 2004-2013
750
^700 '
E 650 f
--600
0 550
500
J 450
400
CO C CO "— fa a3 "— CO " ca — CU ca — M a3 "— M a., ca "— ca ca — aS CO -
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C Ca �r¢�m �r¢�rm v¢�m v¢�m v¢�m
v v v v v d �t �t 4 c1
2004 2005 2006 2007 2008 2009 2011 2013
Figure 4-la. Lengths of fish collected from Waterville Lake for tissue analyses, years 2004-2013.
11

Max, Min and Mean of Weights taken from
Fish Tissue Samples 2004-2013
9000
8000
7000
�6000
s 5000
24000
3000
2000
1000 } JL
0
•+ ++ �+ ++ r r 4 r-. .r+ N r a+ Q ++ •+ i+ .r+ .�.. .F.
f0 � (D � — f0fti � f0 �— Ca ",Cc — M C13 Ca � f6 �— f6 � (0 �— f6 � f0 �—
U f6U N OU c6U c0 OU (OU c6 OU c6() N OU c6U c0 OU c0U cl{ OU f6U c6 OU coU O
�¢v m �¢�tm v¢vm v¢�m Ict �¢1rm
�r �r
. 2004 2005 2006 2007 2008 2009 2011 2013
Figure 4-lb. Weights of fish collected from Waterville Lake for tissue analyses, years 2004-2013.
12

I t
' ✓ 5. SAMPLE PREPARATION
All fish tissue samples were prepared in accordance with U.S. EPA Region IV recommendations
(Cunningham 1990) as described in EA (2001a). To prevent cross-contamination between
sampling stations, all sampling equipment likely to come into contact with the fish was rinsed
extensively with site water between stations.
Specimens collected at each station were sorted by size and species, and target species were
identified. The objective was to obtain a 3- to 5-fish composite sample for channel catfish and
common carp at both 4A and 4B sample locations, as well as a common carp whole body sample
from either station in Waterville Lake which met the species and size objectives discussed in
Section 3. From the target fishes collected, specimens of similar length and weight were selected
for each composite sample. All specimens retained were immediately placed on ice for later
processing. For each fish retained, length and weight data were recorded on the appropriate
fisheries data sheet.
Following identification of target organisms, selection of composite samples, and collection of
length/weight data, each specimen was prepared for shipment and analysis. Bottom feeder fillet
samples consisted of epaxial muscle tissue and skin from one side of the fish. Bottom feeder
whole body samples consisted of the entire fish. Fillet samples were prepared by removing
scales (or removing the skin from catfishes) and then making an incision behind the opercula(on
both sides of the fish) from the base of the spine (behind the skull)to just below the pectoral fin.
Care was taken to cut through the epaxial muscle without puncturing the rib cage or gut lining.
'! A second incision was made along the length of the spine to the caudal fin on both sides of the
fish. The epaxial muscle was then gently cut away from the rib cage to obtain a fillet. In this
fashion, all flesh and skin was obtained from head to tail on both sides of the fish. Fillet knives
were solvent rinsed (hexane and acetone) between fish from different stations. Each composite
sample was wrapped in aluminum foil (dull side toward sample), labeled, and placed on ice in
the field.
All individual specimens (fillets) composing a single composite sample were placed in a water-
tight plastic bag labeled with the station name, sample number, and species identification. A
chain-of-custody form was filled out for each cooler of samples submitted for analysis. Each
form included composite specific information and instructions. All chain-of-custody forms had
the following information:
• sample location and station identification,
• sampling team initials,
• date of collection,
• species name,
• sample type(i.e., fillet or whole body)
A copy of the chain-of-custody record is provided in Appendix A.
All samples were frozen solid prior to shipment to the analytical laboratory. The frozen samples
were packed on dry ice and shipped via overnight delivery, where they arrived on 9/5/13 at
13

' TestAmerica West Sacramento. The back-up fillets were retained in a freezer at the Canton Mill
until laboratory analytical results for the right side fillets were received and verified.
t
14

6. ANALYTICAL RESULTS
The fish tissue samples were received in one shipment (9/5/13) at TestAmerica under chain-of-
custody protocol. Once received at the laboratory, samples were compared to the chain-of-
custody record to verify the content of each shipping container. Each individual fish or fillet
within a composite was homogenized separately by TestAmerica personnel, and equal aliquots
of the homogenate from each fish were removed to constitute the composite. Dioxin and furan
analyses were performed using high resolution Gas Chromatography/Mass Spectrometry
(GC/MS) as required by the U.S. EPA. Laboratory documentation of all chemical extractions
and analyses are provided in Appendix B. All chemical analyses of the samples were conducted
using EPA Method 8290 (U.S. EPA 1994) as specified in the Fish Tissue Sampling Study Plan
(EA 2001a).
The quality of the analytical results was assured through reproducible calibration and testing of
the extractions and GC/MS systems. A laboratory method blank was prepared along with each
batch of samples. The laboratory also used precision and recovery standards for determination
of initial and ongoing precision and accuracy.
Laboratory reports for all 2013 Pigeon River fish tissue dioxin, furan, and lipid content analyses
are provided in Appendix B. Each laboratory analysis report form lists the concentration of
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8,-tetrachlorodibenzofuran (TCDF), and all
other CDD/CDF congeners. Results of the dioxin, furan, and lipid content analyses are
summarized in Table 6-1. Detection limits are reported parenthetically on a sample-specific
basis. Only fillet results are discussed below because North Carolina Department of Health and
Human Services (NC DHHS) considers only fillet results when issuing health advisories.
Concentrations of 2,3,7,8-TCDD and 2,3,7,8-TCDF in channel catfish fillets were below
detection limit(DL) in Station 4A(DL= 0.48 ppt and 0.48 ppt respectively) and were also below
DL at Station 4B (DL = 0.49 for both 2,3,7,8-TCDD and 2,3,7,8-TCDF (Table 6-1)). In
common carp, the 2,3,7,8-TCDD concentration was 1.8 ppt at Station 4A and was detected
(estimated 0.70 ppt, DL= 0.49) in fillet composites collected at Station 4B. For 2,3,7,8-TCDF,
the concentrations in common carp fillets were 2.0 at Station 4A and 2.6 at Station 4B. The
concentration of 2,3,7,8-TCDF in the common carp whole body sample collected at Station 4B
was 2.4 and 5.8 for 2,3,7,8-TCDD and 2,3,7,8-TCDF respectively(Table 6-1).
Examination of the data in Table 6-1 indicates that all fish collected during this study had body
burdens well below the FDA dioxin health advisory level (25 ppt) for fish tissue [as established
and presented in FDA(1981, 1983) and Cordle(1983)].
The NC DHHS recommends an average dioxin toxicity equivalent(TEQ) level of 3 ppt or less in
fish tissue fillets. The NC DHHS issues dioxin fish tissue advisories at an average toxicity
equivalent of 4 ppt or greater(e-mail correspondence with Dr. Luanne Williams 18 January
2005). The TEQ of each chlorodibenzodioxin and furan(CDD/F) isomer is based on the toxicity
equivalence factor(TEF) (WHO 2005 and Table 6-2) as described in the 2001 Study Plan (EA
2001b). The TEQ value is calculated assuming additivity of effects for the individual congeners
of dioxin and furans and is expressed as an "equivalent amount of 2,3,7,8-TCDD" (NC DEHNR
1991). The measured concentration of each CDD/F isomer,when multiplied by its appropriate
TEF, yields the TEQ of that isomer(the toxic concentration of that isomer relativd to the toxicity
of 2,3,7,8-TCDD). In cases where concentrations were below the level of detection, a value of
15

- zero was used in the TEQ calculation. Based on an advisory by the World Health Organization
(e-mail correspondence with Dr. Luanne Williams 20 September 2006),new TEFs were
developed in 2005 by the WHO and are now being used instead of the 1997 TEFs.
16

Table 6-1. Summary of the Pigeon River fish tissue analysis results 2013.
Station Percent
Number Sample ID Composite/Sample Type 2.3,7,8-TCDD(°l 2,3,7,8-TCDF'°) Livid
4A SITE 4A channel catfish 5 fillet samples ND(DL=0.48) ND(DL=0.48) 4.0
SITE 4A common carp 5 fillet samples 1.8 2.0 8.7
4B SITE 4B channel catfish 5 fillet sample ND(DL=0.49) ND(DL=0.49) 2.7
SITE 4B common carp 5 fillet samples 0.70'(DL=0.49) 1.4 4.2
SITE 4B common carp 1 whole body sample 2.4 5.8 15
(a)Units=ppt(parts per trillion)or pg/g(picogram/gram)
(b)Estimated results. Result is less than the reporting limit
(c)Analyte was positively identified,confirmation(CON)analysis used.
(ND)Not detected,concentrations below the instrument detection limit(DL).
17

Table 6-2. Toxicity equivalence factors for CDD/F congeners.
DIOXIN DIBENZOFURAN
Isomer(a) TEF(b) TEF(b)
2,3,7,8-TCDD 1 2,3,7,8-TCDF 0.1
1,2,3,7,8-PeCDD 1 1,2,3,7,$-PeCDF 0.03
2,3,4,7,8-PeCDF 0.3
1,2,3,4,7,8-HxCDD 0.1 1,2,3,4,7,8-HxCDF 0.1
1,2,3,7,8,9-HxCDD 0.1 1,2,3,7,8,9-HxCDF 0.1
1,2,3,6,7,8-HxCDD 0.1 1,2,3,6,7,8-HxCDF 0.1
2,3,4,6,7,8-HxCDF . 0.1
1,2,3,4,6,7,8-HpCDD 0.01 1,2,3,4,6,7,8-HpCDF 0.01
1,2,3,4,7,8,9-HpCDF 0.01
OCDD 0.0003 OCDF 0.0003
(a) In each homologous group, the relative toxicity factor for the congeners not listed is 1/100 of
the value listed for the other congeners in that homologous group.
i (b)TEF=toxic equivalence factor=relative toxicity assigned.
18

The TEQ calculation and summarization schemes presented in Table 6-3 followed methods used
by NC DHHS (NC DEHNR 1991).
All fish fillets analyzed had TEQ values below the NC DHHS advisory limit (3 ppt). For
channel catfish, all TCDD and TCDF congeners analyzed were below detection resulting in a
TEQ of 0.000. The TEQ for common carp was 2.455 at Station A and 0.893 at Station B. The
values reported in years 2005-2011 for channel catfish are consistent with the results for 2013.
Common carp TEQ results in 2007 were higher (Station 4A, 13.44 ppt; Station 4B, 1.263 ppt)
than observed in previous years (2005-2006), and results for 2008 and 2009 (2009, Station 4A,
1.560; Station 4B, 1.496 ppt) are further evidence that the 2007 results were anomalous. The
2013 whole body sample for common carp collected at Station 4B had a TEQ result of 3.196 ppt.
Table 6-4 illustrates 2,3,7,8 TCDD concentrations in common carp fillet samples collected in
2005, 2006, 2007, 2008, 2009, 2011, 2013 and results for previous years (1990-2004) are in
Appendix C. Between 1990 and 2006, 2,3,7,8-TCDD concentrations in carp fillets declined
dramatically(94.99 %) at all sampling stations (Table 6-4,Appendix C, Figure 6-1).
The mean weights of common carp collected from Stations 4A and 4B (5.052 kg, Station 4A;
4.364 kg, Station B) were lower than those collected in 2007 (6.490 kg - Station 4A; 6.552 kg -,
Station 4B), but consistent with weights of common carp collected in previous years (2008,
4.727- Station 4A, 4.067 Station 4B; 2006, 4.505 kg - Station 4A, 5.637 kg - Station 4B; 2005,
3.857 kg- Station 4A, 4.695 kg- Station 4B; 2004, 3.750 kg- Station 4A, 3.900 kg- Station 4B;
1999-2003, 3.193 kg- Station 4A, 5:042 kg - Station 4B). Lipid concentrations in common carp
fillets in 2013 were 4.2-8.7%.
19

Table 6-3. Summary of CDD/F isomer analyses, toxicity equivalent factors, and toxicity equivalent values for the 2013 Waterville
Lake fish tissue composites.
STATION 4A
Channel catfish fillet Common cart)fillet
CDD/F congeners TEF(`) Results('! T Results") TEOro>
Dibenzodioxin
2,3,7,8-TCDD 1.0 ND 0.000 1.8 1.8
1,2,3,7,8-PeCDD 1.0 ND 0.000 ND 0.000
1,2,3,4,7,8-HxCDD 0.1 ND 0.000 ND 0.000
1,2,3,6,7,8-HxCDD 0.1 ND 0.000 3.2" 0.32
1,2,3,7,8,9-HxCDD 0.1 ND 0.000 ND 0.000
1,2,3,4,6,7,8-HpCDD 0.01 ND 0.000 12 0.12
OCDD 0.0003 ND 0.000 50 0.015
Dibenzofuran
2,3,7,8-TCDF 0.1 ND 0.000 2.0 0.20
1,2,3,7,8-PeCDF 0.03 ND 0.000 ND 0.000
2,3,4,7,8-PeCDF 0.3 ND 0.000 ND 0.000
1,2,3,4,7,8-HxCDF 0.1 ND 0.000 ND 0.000
1,2,3,6,7,8-HxCDF 0.1 ND 0.000 ND 0.000
2,3,4,6,7,8-HxCDF 0.1 ND 0.000 ND 0.000
1,2,3,7,8,9-HxCDF ' 0.1 ND 0.000 ND 0.000
1,2,3,4,6,7,8-HpCDF 0.01 ND 0.000 ND 0.000
1,2,3,4,7,8,9-HpCDF 0.01 ND 0.000 ND 0.000
OCDF 0.0003 ND 0.000 ND 0.000
Total TEQ 0.000 2.455
(a)Units=ppt(parts per trillion)or pglg(picogram per gram)
(b)Dioxin Equivalent Concentration using methodology from U.S.EPA(1989)
(c)Toxicity Equivalent Factors from World Health Organization(WHO 2005)
(d)Estimated result. Result is less than reporting limit.
20

r
Table 6-3 (cont). Summary of CDD/F isomer analyses,toxicity equivalent factors, and toxicity equivalent values for the 2013
Waterville Lake fish tissue composites.
STATION 4E
Channel catfish fillet Common cart)fillet Common carp whole body
CDD/F congeners TEFt`) Results TEOtb� Results(') TEOc"� Resul[st°� TEOro>
Dibenzodioxin
2,3,7,8-TCDD 1.0 ND 0.000 0.70 0.76d 2.4 2.4
1,2,3,7,8-PeCDD 1.0 ND 0.000 ND 0.000 ND 0.000
1,2,3,4,7,8-HxCDD 0.1 ND 0.000 ND 0.000 ND 0.000
1,2,3,6,7,8-HxCDD 0.1 ND 0.000 ND 0.000 4.9 0.49
1,2,3,7,8,9-HxCDD 0.1 ND 0.000 ND 0.000 ND 0.000
1,2,3,4,6,7,8-HpCDD 0.01 ND 0.000 4.9 0.049 15 0.15
OCDD 0.0003 ND 0.000 14 0.0042 58 0.016
Dibenzofuran
2,3,7,8-TCDF 0.1 ND 0.000 1.4 0.14 1.4 0.14
1,2,3,7,8-PeCDF 0.03 ND 0.000 ND 0.000 ND 0.000
2,3,4,7,8-PeCDF 0.3 ND 0.000 ND 0.000 ND 0.000
1,2,3,4,7,8-HxCDF 0.1 ND 0.000 ND 0.000 ND 0.000
1,2,3,6,7,8-HxCDF 0.1 ND 0.000 ND 0.000 ND 0.000
2,3,4,6,7,8-HxCDF 0.1 ND 0.000 ND 0.000 ND 0.000
1,2,3,7,8,9-HxCDF 0.1 ND 0.000 ND 0.000 ND 0.000
1,2,3,4,6,7,8-HpCDF 0.01 ND 0.000 ND 0.000 ND 0.000
1,2,3,4,7,8,9-HpCDF 0.01 ND 0.000 ND 0.000 ND 0.000
OCDF 0.0003 ND 0.000 ND 0.000 ND 0.000
Total TEQ 0.000 0.893 3.196
(a)Units=ppt(parts per trillion)or pg/g(picogram per gram)
(b)Dioxin Equivalent Concentration using methodology from U.S.EPA(1989)
(c)Toxicity Equivalent Factors from World Health Organization(WHO 2005)
(d)Estimated result. Result is less than reporting limit.
21

Table 6-4. Blue Ridge Paper Canton Mill fish fillet tissue analysis results,2005—2013
2005 Results(d) 2006 Results(e)
Number Length Range Number Length Range
Station Species of fish (mm) 2,3,7,8-TCDD(0." Station Species of fish (mm) 2,3,7,84CDDt4"1
1 Black redhorse 5 359-384 ND(0.35) 4A Common carp 3 635-692 0.64 t`I
RM 64.5 RM 41.5 Channel catfish 5 430-500 ND(0.37)
2 Common carp 4 595-650 ND(0.45) 4B Common carp 3 650-745 0.73 t`I
RM 59.0 Channel catfish 3 443460 ND(0.29) RM 39.0 Channel catfish 4 510-590 0.77(`)
3 Common carp 4 605-630 ND(0.46)
RM 52.3
4A Common carp 3 601-665 1.3
RM 41.5 Channel catfish 5 464-508 ND(0.36)
4B Common carp 4 595-736 2.8
RM 39.0 Channel catfish 5 513-603 0.76t`l
5 Black redhorse 4 440-501 ND(0.41)
RM 19.0
Total fish filleted= 37 Total fish filleted= 15
(a)Dioxin analyses conducted by TestAmerica(formerly Severn Trent Laboratories).
(b)Units=ppt(parts per trillion)or pg/g(picogram/gram)
(c)Estimated result. Result is less than the reporting limit.
(d)Survey conducted in August and September
(e)Survey conducted in August
ND=Non-detectable at the detection limit in parentheses
22

C; F C
Table 6-4 (cont). Blue Ridge Paper Canton Mill fish fillet tissue analysis results, 2005—2013
2007 Results(d) 2008 Results(e)
Number Length Range Number Length Range
Station Species offish (mm) 2,3,7,8-TCDDjab1 Station Species offish (mm) 2,3,7,8-TCDD(4b)
4A Common carp 4 680-775 6.4 4A Common carp 3 653-686 1.2
RM 41.5 Channel catfish 4 490-593 0.57t`t RM 41.5 Channel catfish 5 475-525 ND(0.29)
4B Common carp 5 672-762 ND(6.9) 4B Common carp 3 630-665 ND(0.36)
RM 39.0 Channel catfish 5 505-670 0.67`I RM 39.0 Channel catfish 5 459-595 ND(0.34)
Total fish filleted= 18 Total fish filleted= 16
(a)Dioxin analyses conducted by TestAmerica(formerly Severn Trent Laboratories).
(b)Units=ppt(parts per trillion)or pg/g(picogram/gram)
(c)Estimated result. Result is less than the reporting limit.
(d)Survey conducted in August and September
(e)Survey conducted in August
ND=Non-detectable at the detection limit in parentheses
23

Table 6-4 (cont). Blue Ridge Paper Canton Mill fish fillet tissue analysis results, 2005 —2013
2009 Results(d) 2011 Results(e)
Number Length Range Number Length Range
Station Species of fish (mm) 2,3,7,8-TCDD'3,bi Station Species of fish (mm) 2,3,7,8-TCDDIN)
4A Common carp 5 620-698 1.1 4A Common carp 5 625-710 0.83`
RM 41.5 Channel catfish 3 493-578 0.52t`l RM 41.5 Channel catfish 5 435-478 ND(0.24)
4B Common carp 5 647-696 0.93'`I 4B Common carp 5 625-740 1.1
RM 39.0 Channel catfish 5 467-574 ND(0.43) RM 39.0 Channel catfish 5 466-550 1.7
Total fish filleted= 18 Total fish filleted= 20
(a)Dioxin analyses conducted by TestAmerica(formerly Sevens Trent Laboratories).
(b)Units=ppt(parts per trillion)or pg/g(picogram/gram)
(c)Estimated result. Result is less than the reporting limit.
(d)Survey conducted in August and September
(e)Survey conducted in August
ND=Non-detectable at the detection limit in parentheses
24

Table 6-4 (cont). Blue Ridge Paper Canton Mill fish fillet tissue analysis results,2005 —2013
2013 Results(d)
Number Length Range
Station Species offish (mm) 2,3,7,8-TCDD(ab)
4A Common carp 5 631-722 1.8
RM 41.5 Channel catfish 5 391-511 ND(0.48)
413 Common carp 5 630-697 0.701`l
RM 39.0 Channel catfish 5 445-537 ND(0.49)
Total fish filleted= 20
(a)Dioxin analyses conducted by TestAmerica(formerly Severn Trent Laboratories).
(b)Units=ppt(parts per trillion)or pg/g(picogram/gram)
(c)Estimated result. Result is less than the reporting limit.
(d)Survey conducted in August
ND=Non-detectable at the detection limit in parentheses
25

Figure 6-1. TCDD Concentrations in Carp Fillets Collected from the Pigeon River,
1990-2013
(Stations 4A and 413)
70
60
Station 4A
50
--or- Station 48
0
40
Z
d
a �
W 30
m
a
0 20 -
O �
F �
10 A� �
A
i
0 ,
1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013
TIME
b) TCDD concentrations at Station 4B were not detected,therefore,the values plotted represent the detection limit for those samples.
The detection limit for 2,3,7,8-TCDD was elevated in the 2007 carp fillet for Station 4B because of a 10-fold dilution of the sample
which was required to overcome sample matrix effects(high lipid concentration) during analysis. Consequently, reporting the
detection limit for this sample can give a misleading impression that the concentration of 2,3,7,8-TCDD increased that year
26

Figure 6-2. TCDD Concentrations in Channel Catfish Fillets Collected from the Pigeon River,
1997-2013
(Stations 4A and 4B)
2.50
+Station 4A
2.00 -- - -
- t- Station 4B
o c
Y
d 1.50 \\
a ; .,
Yl �
M j `\
A � \
a 1.00
0
a b
U �---�_, b
I- A i �\ a `\
a \
0.50 a I----�_, a a a --A a
b ��__ b b
a
0.00 _ _ - —
1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013
TIME
a) TCDD concentrations were not detected at Station 4A, therefore the values plotted represent the detection limit for the sample.
b) TCDD concentrations were not detected at Station 413, therefore the values plotted represent the detection limit for the sample.
c) The 2011 TCDD result for catfish fillets in 2011 at Station 4B was above detection limit at 1.7 ppt . The TEQ for the 2011 Station
4B catfish fillets remained below the 4 ppt action level of the NC DHHS.
27

• 7. REFERENCES
Curdle, F. 1983. Use of epidemiology in the regulation of dioxins in the food supply, in
Accidental Exposure to Dioxins: Human Health Aspects (F. Coulston and F. Pocchiara,
eds.),pp 245-256. Academic Press,New York.
Cunningham, W.R. 1990. Letter to Paul Wiegand. 30 January:
EA Engineering, Science, and Technology Inc. 1990. Study Plan for the Monitoring of Dioxin
in Fish Tissue. EA Report No. 11370.01,prepared for Champion International Corporation,
Canton,North Carolina.
EA Engineering, Science, and Technology Inc. 1991. Results of the 1990 Dioxin Monitoring in
Fish Tissue. EA Report No. 11370.02, prepared for Champion International Corporation,
Canton,North Carolina.
EA Engineering, Science, and Technology Inc. 1992. Results of the 1991 Dioxin Monitoring in
Fish Tissue. EA Report No. 11370.03, prepared for Champion International Corporation,
Canton,North Carolina.
EA Engineering, Science, and Technology hie. 1993a. Results of the 1992 Dioxin Monitoring
in Fish Tissue. EA Report No. 11370.05,prepared for Champion International Corporation,
Canton,North Carolina. April.
EA Engineering, Science, and Technology hie. 1993b. Results of the 1993 Dioxin Monitoring
in Fish Tissue. EA Report No. 11370.06,prepared for Champion International Corporation,
Canton,North Carolina. December.
EA Engineering, Science, and Technology Inc. 1994. Results of the 1994 Dioxin Monitoring in
Fish Tissue. EA Report No. 11370.07, prepared for Champion International Corporation,
Canton,North Carolina. December.
EA Engineering, Science, and Technology hie. 1995. Results of the 1995 Dioxin Monitoring in
Fish Tissue. EA Report No. 13043.01, prepared for Champion International Corporation,
Canton,North Carolina. December.
EA Engineering, Science, and Technology hie. 1996. Results of the 1996 Dioxin Monitoring in
Fish Tissue. EA Report No. 13176.'01, prepared for Champion International Corporation,
Canton,North Carolina. December.
EA Engineering, Science, and Technology hie. 1997. Results of the 1997 Dioxin Monitoring in
Fish Tissue. EA Report No. 13353.01, prepared for Champion International Corporation,
Canton,North Carolina. November.
t..J
28

EA Engineering, Science, and Technology Inc. 1998. Results of the 1998 Dioxin Monitoring in
Fish Tissue. EA Report No. 13478.01, prepared for Champion International Corporation,
Canton,North Carolina. December.
EA Engineering, Science, and Technology Inc. 2000. Results of the 1999 Dioxin Monitoring in
Fish Tissue. EA Report No. 13478.01, prepared for Champion International Corporation,
Canton,North Carolina. January.
EA Engineering, Science, and Technology Inc. 2001a. Study Plan for Pigeon River Dioxin
Monitoring in Fish tissue. Prepared for Blue Ridge Paper Products Inc., Canton, North
Carolina. November.
EA Engineering, Science, and Technology Inc. 2001b. Results of the 2000 Dioxin Monitoring
in Fish Tissue. EA Report No. 13745.01, prepared for Blue Ridge Paper Products Inc.,
Canton,North Carolina. December.
EA Engineering, Science, and Technology Inc. 2001c. Results of the 2001 Dioxin Monitoring
in Fish Tissue. EA Report No. 13900.01, prepared for Blue Ridge Paper Products Inc.,
Canton,North Carolina. December.
EA Engineering, Science, and Technology Inc. 2003. Results of the 2002 Dioxin Monitoring in
Fish Tissue. EA Report No. 13900.02, prepared for Blue Ridge Paper Products Inc.,
Canton,North Carolina. December.
EA Engineering, Science, and Technology Inc. 2004. Results of the 2003 Dioxin Monitoring in
Fish Tissue. EA Report No. 13900.03, prepared for Blue Ridge Paper Products Inc.,
Canton,North Carolina. January.
Food and Drug Administration. 1981. FDA advises Great Lakes States to monitor dioxin-
contaminated fish. FDA Talk Paper dated 28 August, in Food Drug Cosmetic Law
Reports,paragraph 41, 321. Commerce Clearing House, Inc. 8 September.
Food and Drug Administration. 1983. Statement by Stanford A. Miller, Director, Bureau of
Foods, FDA before the Subcommittee on Natural Resources, Agriculture Research and
Environment,U.S. House of Representatives. 30 June.
Henry, T.B., and J.L. Wilson. 2006. Results of 2005 dioxin monitoring in fish tissue. Prepared
for Blue Ridge Paper Products, Inc., Canton Mill, Canton,North Carolina. January 2006.
Henry, T.B., and J.L. Wilson. 2006. Results of 2006 dioxin monitoring in fish tissue. Prepared
for Blue Ridge Paper Products, Inc., Canton Mill, Canton,North Carolina. December 2006.
Henry, T.B., and J. L. Wilson. 2008. Results of 2007 dioxin monitoring in fish tissue. Prepared
for Blue Ridge Paper Products, Inc.; Canton Mill, Canton,North Carolina. April 2008.
29

Henry, T.B., and J. L.Wilson. 2008. Results of 2008 dioxin monitoring in fish tissue. Prepared
for Blue Ridge Paper Products, Inc., Canton Mill, Canton,North Carolina. November 2008.
Henry,T.B., and J. L. Wilson. 2009. Results of 2009 Dioxin Monitoring in Fish Tissue. Final
Report to Blue Ridge Paper Products, Inc., dba Evergreen Packaging. December 2009. 29
Pp-
Henry,T.B., and J. L. Wilson. 2011. Results of 2011 Dioxin Monitoring in Fish Tissue. Final
Report to Blue Ridge Paper Products, Inc., dba Evergreen Packaging. December 2011. 29
pp-
North Carolina Department of Environment, Health, and Natural Resources. 1991. Fish Tissue
Dioxin levels in North Carolina: 1990 update. Division of Environmental Management,
Water Quality Section.
U.S. Environmental Protection Agency (U.S. EPA). 1994. Analytical procedures and Quality
Assurance for Multimedia Analysis of Polychlorinated Dibenzo-para-dioxins and
Polychlorinated Dibenzofurans by High-Resolution Gas Chromatography/High-Resolution
Mass Spectrometry(Method 8290).
U.S. Environmental Protection Agency (U.S. EPA). 1989. Interim Procedures for Estimating
Risk Associated with Exposures to Mixtures of Chlorinated Dibenzo-p-dioxins and
- Dibenzofurans (CDDs and CDFs) and 1989 Update Report No. EPA/625/3-89/016, U.S.
i EPA, Risk Assessment Forum,Washington, D.C.
Versar, Inc. 1984. Sampling Guidance Manual for the National Dioxin Study. U.S.
Environmental Protection Agency. EPA Contract 68-01-6160. Work Order Number 8.7.
Office of Water Regulations and Standards, Monitoring and Data Support Division,
Washington, D.C. Final Draft. July.
Wilson, J.L. 2006. Study plan revision: Dioxin monitoring in fish tissue, Waterville Lake.
Prepared for Blue Ridge Paper Products, Inc. Canton Mill, Canton,North Carolina. March
2006.
World Health Organization (WHO). 1997. Exposure and Human Health Reassessment of
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and Related Compounds: Part 11: Health
Assessment of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and Related Compounds.
30

APPENDIX A: CHAIN OF CUSTODY FORM
APPENDIX B: TESTAMERICA WEST SACRAMENTO ANALYTICAL REPORT
APPENDIX C: BLUE RIDGE PAPER CANTON MILL FISH FILLET
TISSUE ANALYSIS RESULTS 1990-2004
APPENDIX D: 2006 REVISIONS TO DIOXIN MONITORING PLAN
Appendices to the 2013 dioxin in fish tissue report are in separate PDF file document
31

APPENDIX A
CHAIN OF CUSTODY FORM
l.�
32

TestAmer)ca Sacramento Chain of Cui tody Record Te$t encq
88D Riverside Parkway IlIe1,1a'111'm,,IltI1IIm1111It ;
�� 'D91111 'NE LFAam IN NwRONMEMAL TEBTINa
West Sacramento.CA 95605 I NIII1tID111It'Lllmly111 '
phone [phone] fax 916.372 tosa Regulatory Program;.. ❑DW ❑+ ❑NPOF3 Rau ❑Olh I 'estArrieric tLaboratorles,Inc. $
Client contact Project Manager. Nick McCracken _ Site COM :Dr.Le. 113203948 Chant of Cus o�fy •- )C No' 0 2013
Evergreen Packaging. - - Tdffax:(S28)8462874 - lab Contact 1 of • 1 coca
175 Main Street - AnaWn Turnaround Time ampler.Univ rally of Tennessee
Carton,NC 28716 ❑ CALENDAR DAYS ❑WORMG DAYS For Lab Use I Mr.
828 646-6700 Phone TAT 0 ddaantevm Below x alkm Client(628)646-6892 FAX ❑ 2 weeo = y �b Sampling.
Project Name,Fish Tissue Sampling ❑ 1 week
Site.Waterville Lake ❑ 2da/s •q M g1 I oe No.
PO# _. _ ❑ Ida/ - E m it LTk
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Semple Sample l F our $ f
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APPENDIX B
TESTAMERICA WEST.SACRAMENTO ANALYTICAL
REPORT
33

TestAmenca
THE LEADER IN ENVIRONMENTAL TESTING
ANALYTICAL REPORT
TestAmerica Laboratories, Inc.
TestAmerica Sacramento
880 Riverside Parkway
West Sacramento, CA 95605
Tel: [phone]
TestAmerica Job ID: 320-3948-1
Client Project/Site: Tissue
For:
Evergreen Packageing FKABlue Ridge Paper
PO BOX 1549
Canton, North Carolina 28716
Attn: Nick McCracken
Authorized for release by:
113012014 2:56:57 PM
Jill Kellmann, Senior Project Manager
[phone]
[e-mail]
LINKS
Review your project
results through
TotalAcce%
Have a Question?
Ask
/The This report has been electronically signed and authorized by the signatory. Electronic signature is
intended to be the legally binding equivalent of a traditionally handwritten signature.
Ex ert
Results relate only to the items tested and the sample(s)as received by the laboratory.
Visit us at:
www.testamericai ncDOM

Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID:320-3948-1
Project/Site:Tissue
Table of Contents ��
CoverPage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Table of Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 F1
Definitions/Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Case Narrative . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 ❑
Detection Summary °
Client Sample Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Isotope Dilution Summary . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . 12
QC Sample Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 °
QC Association Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 d
Lab Chronicle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 410
Certification Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Method Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Sample Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Chain of Custody . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Receipt Checklists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
i
TestAmedca Sacramento
Page 2 of 22 1/30/2014

Definitions/Glossary
Client: Evergreen Packageing FKABlue Ridge Paper TestAmenca Job ID: 320-3948-1
Project/Site:Tissue
Qualifiers
Dioxin
Qualifier Qualifier Description
H Sample was prepped or analyzed beyond the specified holding time
J Result is less than the RL but greater than or equal to the MDL and the concentration is an approximate value.
q The isomer is qualified as positively identified,but at an estimated quantity because the quantitation is based on the theoretical ratio for
these samples.
Glossary
Abbraviatlat Thesis commonly used abbreviations may or may not be pnasint In Oft report
o Listed under the"D"column to designate that the result is reported on a dry weight basis
%R Percent Recovery
CNF Contains no Free Liquid
DER Duplicate error ratio(normalized absolute dMerence)
Di Fac Dilution Factor
DL,RA,RE,IN Indicates a Dilution,Re-analysis,Re-exlmction,or additional Initial metals/amon analysis of the sample
DLC Decision level concentration
MDA Minimum detectable activity
EDL Estimated Detection Limit
MDC Minimum detectable concentration
MDL Method Detection Limit
MIL Minimum Level(Dioxin)
NC Not Calculated
ND Not detected at the reporting limit(or MDL or EDL ff shown)
PQL Practical Quantitabon Limit
QC Quality Control
RER Relative eror ratio
RL Reporting Limit or Requested Limit(Radiochemistry)
RPD Relative Percent Difference,a measure of the relative difference between two points
TEF Toxicity Equivalent Factor(Dioxin)
TEQ Toxicity Equivalent Quotient(Dioxin)
TestAmerica Sacramento
Page 3 of 22 1/30/2014

Case Narrative
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID: 320-3948-1
Project/Site:Tissue
Job ID: 3203948-1
Laboratory:TestAmerica Sacramento
Na aft
CASE NARRATIVE ,
320-3948-1
Receipt
The samples were received on 9/5/2013 8:15 AM;the samples arrived in good condition,properly preserved and where required.on ice.
Except:
Received fish samples frozen solid from dry ice. The laboratory IR thermomenter could not read at that low of a range.
Dioxin-Method 8290
The Ion abundance ratios are outside criteria for one or more analytes in the following sample:SITE 4A COMMON CARP R-FILLETS
(320-3948-2). Ouanfitation is based on the theoretical ion abundance ratio;therefore,these analytes have been reported as an estimated
maximum possible concentration(EMPC). The affected analytes have been flagged.
No other analytical or quality issues were noted.
Dioxin Prep
The following samples were prepared outside of preparation holding time:COMPOSITE OF 4B CHANNEL CAT#3,5(320-3948-8),
COMPOSITE OF 4B COMMON CARP #4,6(320-3948-9).SITE 4A CHANNEL CAT R-FILLETS(320-3948-1),SITE 4A COMMON CARP
R-FILLETS(320-3948-2),SITE 4B COMMON CARP WB(320-3948-7). Samples were received at the laboratory on 9/5/13. The method
8290 preparation holding fines expired 914113 and 9/5113, Sample extraction was initiated 9/12/13.
No other analytical or quality issues were noted.
TestAmerica Sacramento
Page 4 of 22 1/30/2014

Detection Summary C
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID:320-3948-1
Project/Site:Tissue
Client SampleSample ID: SITE 4A CHANNEL CAT R-FILLETS Lab Sample ID: 320-3948-1 1 1;
L
FAnalyte Result Qualifier NONE NONE Unit Dil Fac D Method Prep Type
Percent Lipids 4.0 % 1 Lipids Total/NA �47
Client Sample ID: SITE 4A COMMON CARP R-FILLETS Lab Sample ID: 320-3948-2 (©
Analyte Result Qualifier NONE NONE Unit DII Fac D Method Prep Type L"9
Percent Lipids 8.7 % 1 Lipids Total/NA
Analyte Result Qualifier RL TDL Unit DII Fac D Method Prep Type IY
OCDD 50 H 9.8 4.9 pg/g 1 8290 TotaIMA r
Total TCDF 3.0 H 0.98 0.49 pg/g 1 8290 Total/NA iLLr�}=_
1,2,3,6,7,8-HxCDD 3.2 J H 4.9 2.4 pg/g 1 8290 Total/NA
Total TCDD 1.8 H 0.98 0.49 pg/g 1 8290 Total/NA
I9
Total PeCDF 2.8 J H q 4.9 2.4 pg/g 1 8290 Total/NA
Total HpCDD 15 H 4.9 2.4 pg/g 1 8290 TotaVNA 7tJa
Total HpCDD 3.2 J H 4.9 2.4 pg/g 1 8290 Total/NA ---
1,2,3,4,6,7,8-HpCDD 12 H 4.9 2.4 pg/g 1 8290 Total/NA
2,3,7,8-TCDD 1.8 H 0.98 0.49 pg/g 1 8290 TotaIMA
2,3,7,8-TCDF 2.0 H 0.98 0.49 pg/g 1 8290 Total/NA
Client Sample ID: SITE 4B COMMON CARP WB Lab Sample ID: 320-3948-7 4
Analyte Result Qualifier NONE NONE Unit DII Fee D Method Prep Type I i.
'„
Percent Lipids 15 % 1 Lipids Total/NA
1J
Analyte Result Qualifier RL TOL Unit DII Fee D Method Prep Type lid,
OCDD 58 H 9.7 4.8 p9/9 1 8290 Total/NA -
TotaITCDF 7.1 H 0.97 0.48 pg/g 1 8290 ToteVNA
1,2,3,6,7,8-HxCDD 4.9 H 4.8 2.4 pg/g 1 8290 TotaIMA
Total TCDD 2.4 H 0.97 0.48 pg/g 1 8290 Total/NA
Total PeCDF 3.8 J H 4.8 2.4 pg/g 1 8290 Total/NA
Total HpCDD 15 H 4.8 2.4 pg/g 1 8290 Total/NA
Total HpCDD 4.9 H 4.8 2.4 pg/g 1 8290 TotaIMA
1,2,3,4,6,7,8-HpCDD 15 H 4.8 2.4 pg/g 1 8290 TotaIMA
2,3,7,8-TCDD 2.4 H 0.97 0.48 pg/g 1 8290 TDtaIMA
2,3,7,8-TCDF 5.8 H 0.97 0.48 pg/g 1 8290 TotaIMA
Client Sample ID: COMPOSITE OF 4B CHANNEL CAT#3,5 Lab Sample ID: 320.3948-8
rAnalyte Result Qualifier NONE NONE Unit Dil Fac D Method Prep Type
Percent Lipids 2.7 % 1 Lipids Total/NA
Client Sample ID: COMPOSITE OF 4B COMMON CARP #4,6 Lab Sample ID: 320-3948-9
Analyte Result Qualifier NONE NONE Unit DII Fee D Method Prep Type
Percent Lipids 4.2 % 1 Lipids Total/NA
Analyte Result Qualifier RL TDL Unit DII Fee D Method Prep Type
OCDD 14 H 9.8 4.9 p9/9 1 8290 TotalMA
Total TCDF 1.7 H 0.98 0.49 pg/g 1 8290 TotaVNA
Total TWO 0.70 J H 0.98 0.49 pg/g 1 8290 7otalMA
Total HpCDD 4.9 H 4.9 2.5 pg/g 1 8290 Total/NA
1,2,3,4,6,7,8-HpCDD 4.9 H 4.9 2.5 p9/9 1 8290 Total/NA
2,3,7,8-TCDD 0.70 J H 0.98 0.49 pg/g 1 8290 Total/NA
This Detection Summary does not include radiochemical test results.
TestAmerica Sacramento
Page 5 of 22 1/30/2014

Detection Summary
Client Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID:320-3948-1
I
- - Project/Site:Tissue
` Client Sample ID: COMPOSITE OF 4B COMMON CARP #4,6 Lab Sample ID: 320-3948-9 [J
(Continued)
t
Analyte Result Qualifier RL TDL Unit DII Fac D Method Prep Type
2,3,7,8-TCDF 1.4 H 0.98 0.49 pg/g 1 8290 Total/NA
1-61
T/
0
1-�
RAI
This Detection Summary does not include radiochemical test results.
TestAmerica Sacramento
Page 6 of 22 1/30/2014

Client Sample Results
Client: Evergreen Packageing FKABlue Ridge Paper TestAmenca Job ID: 320-3948-1
Project/Site:Tissue
Client Sample ID: SITE 4A CHANNEL CAT R-FILLETS Lab Sample ID: 320-3948-1
Date Collected:08/05/13 14:00 Matrix:Tissue
Date Received:09/06/13 08:15 _
Method:8290-Dioxins and Furans(HRGC/HRMS)
ArWyu Result Qualifier RL TDL Una D Prepared Analyzed Dil Fac
OCDD ND H 9.6 4.8 pg/g 09/12/1312:21 09/1811320:41 7
Total PeCDD NO H 48 2.4 P9/9 09/12/13 12:21 09/18/13 20:41 1
1,2,3,7,8,9-HxCDD NO H 4.8 2.4 P9/9 09/1211312:21 09/1811320:41 1
1,2,3,4,7,8,9-HpCDF ND H 4.8 2.4 pg/g 09/1211312:21 09/18/1320:41 1
1,2,3,4,6,7,8-HpCDF NO H 4.8 2.4 P9/9 09/1211312:21 09118/1320:41 1
TOW TCDF NO H 0.96 048 P9/9 09/12/1312:21 09/18M320:41 1
TOW HpCDF NO H 48 2.4 pWg 09/12/13 12:21 09/18/13 20:41 1
1,2,3,7,8-PeCOD NO H 4.8 2.4 P9/9 0911211312:21 09/18/1320:41 1
1,2,3,7,8-PeCDF NO H 4.8 2A p9/9 09112/1312:21 09/1811320:41 1
1,2,3,6,7,8-HxCDD NO H 48 2A P9/9 09/12/1312:21 09/1811320:41 1
1,2,3,6,7,8-HpCDF NO H 4.8 2.4 P9/9 09/1211312:21 09/18/1320:41 1
Total TWO NO H 0.96 0.48 P9/9 09/12/1312:21 09/1 aft 320:41 1
1,2,3,7,8,9-HxCDF NO H 4.8 2A pg/9 0911211312:21 09118/1320:41 1
OCDF NO H 9.6 4.8 P919 09/12/1312:21 09/1811320:41 1
Total PeCDF NO H 4.8 2A pg/g 0911211312:21 09/1811320:41 1
TON HpCDD NO H 4.8 2A P9/9 09/171131221 0911811320:41 1 - -
1,2,3,4,7,8-HxCDD NO H 4.8 2.4 pg/9 09117J1312:21 09118/1320:41 t
TON HxCDD NO H 4.8 2.4 pWg 09/12J1312:21 09/1811320:41 1
Total HpCDF NO H 4.8 2.4 pg/g 09112/1312:21 09/1W1320:41 1
1,2,3,4,7,8-HxCDF NO H 4s 2A pyg 09/12/13 12:21 09/18/13 20:41 1
1,2,3,4,6,7,8-HpCDD NO H 4s 2.4 pW9 09/12/1312:21 09118/1320:41 1
2.3,4,6.7,8-HxCDF NO H 4.8 2A pg/g M12/1312:21 09/18/1320:41 1
2,3,7,8-TCDD NO H 0.96 0.48 p9/g 09112/1312:21 091181132041 1
2,3,7,84CDF NO H 0.96 0.48 P9/9 09/12/1312:21 0911811320:41 1
2,3,4,7,8-PeCDF ND H 4.8 2.4 pg/g 09/12/1312*21 0911811320:41 1
ko"Dilution %Recovery Qual/1Nr Lbrdtr Prepared Ansyzed Da Fac
13C-1,2,3,4,7,8-HpCDF 62 40- 335 09/12/1312:21 09118H32041 1
13G1,2,3,6,7,8-HxCDD 68 40-135 0911211312.21 09/1W320.41 1
13C-2.3,7,8-TCDD 66 40-135 09/12/1312:21 0911811320.41 1
13C-1,2,3,7,8-PeCDO 69 40-135 09/1211312.21 09/110320.41 1
13C.1,2,3,4,6,7,8-HpCDF 66 40-136 0911211312.21 0911W320:41 1
1 /3C-1,2,3,7,8-PeCDF 63 40-135 09//2/1312:21 0911W320.41 1
13C-2,3,7,8-TCDF 64 40-135 0911211312:21 OW18/1320.41 1
13C-1,$3,4,6,7,8-HpCDD 68 40-135 0911211312.21 0911W320.41 /
13C-OCDD 72 40-135 M211312.21 09/1811320.41 1
General Chemistry
Anayte Result Qualifier NONE NONE Una D Prepared Analyzed Oil Fac
Percent Lipids 4.0 % OW1211312:21 OW13/1311:07 i
Client Sample ID: SITE 4A COMMON CARP R-FILLETS Lab Sample ID: 320-3948-2
Date Collected:08105/13 16:20 Matrix:Tissue
Date Received:09/05/13 08:15
Method:8290-Dioxins and Furans(HRGC/HRMS)
Am" Result Qualifier RL TDL Una D Prepared Analyzed oil Fac
OCDD 50 H 9.8 4.9 pg/p 09/1211312:21 09/18/1321:24 1
Total PeCDD ND H 4.9 2.4 pg/g 09112113 1211 09/18/13 21:24 1
1,2,3.7,8,9-HxCDD NO H 4.9 2.4 pyp OW12JI312:21 09/18/1321:24 1
TestAmenca Sacramento
Page 7 of 22 1/30/2014

Client Sample Results
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID: 320-3948-1
Project/Site:Tissue
Client Sample ID: SITE 4A COMMON CARP R-FILLETS Lab Sample ID: 320-3948-2
Date Collected: 08/05/13 16:20 Matrix: Tissue
Date Received:09/05/13 08:16
Method:8290-Dioxins and Furans(HRGC/HRMS)(Continued)
Arlalyre Result Ouallfler RL TDL UnR D Prepared Analyzed Dil Fac
1,2,3,4,7,s9-HpCDF ND H 4.9 2.4 pg/g 09/12/1312:21 09/18/1321:24 1
1,2,3,4,6,7,8-HeCDF ND H 4.9 2.4 p9/9 09/1211312:21 09/18/1321:24 1 r
Total TCDF 3.0 H 0.98 0.49 pg/g 09/12/13 12:21 09/18/13 21:24 1
Total HxCDF ND H 4.9 2.4 P9/9 09/12/13 12:21 09118/13 21:24 1
1,2,3,7,8-PeCDD ND H C9 2.4 pg/g 09/12/13 12:21 09/18113 21:24 1
1,2,3,7,8-PeCDF ND H 49 2.4 P9/9 09/12/1312:21 OW18/1321:24 1
1,2,3,6,1,8-1xCDD 3.2 JH 4.9 2.4 pg/g 09/1211312:21 09118/13 21:24 1
1,2,3,s7,8-HxCDF ND H 4.9 2.4 P9/9 09/12/1312:21 09/18/1321:24 1
Total TCDD 1.8 H 0.98 0.49 p9/9 09/1211312:21 09/18/1321:24 1
1,2,3,7.8,9.HxCDF ND H 4.9 2.4 p9/9 09/1211312:21 0911811321:24 1
OCDF ND H 9.8 4.9 P9/9 09/1211312:21 09/18/1321:24 1
Total PeCDF 2.8 J H q 4.9 2.4 p91g 09/12/13 12:21 09118/13 21:24 1
Total HpCDD 15 H 4.9 2.4 1>9/9 0911211312:21 09/18/1321:24 1
1,2,3,4,7,8-HxCDO ND H 4.9 2.4 P9/9 0911211312:21 09/1811321:24 1
Total HpCDD 3.2 J H 4.9 2.4 P9/9 0911211312:21 09/18/13 21:24 1
Total HpCDF ND H 4.9 2.4 p9/9 091121131221 09/18/1321:24 1 -
1,2,3,4,7,8-HxCDF ND H 4.9 2.4 pg/g 0911211312:21 0911811321:24 1 `
1,2,3,4,6,7,8-HpCDD 12 H 4.9 2.4 P919 09/12/1312:21 09118/13 21:24 1
2,3,4,6,7,8-HxCDF ND H 4.9 2.4 pg/9 09/1211312:21 09/1811321:24 1
2,3,7,8-TCDD 1.8 H 0,98 0.49 Py9 09/12/1312:21 09/18/1321:24 1
2,3,7,8-TCDF 2.0 H 0.98 0.49 P9/9 0911211312:21 0912011302:30 1
2.3,4,7,8-PeCDF ND H 4.9 2.4 p9/9 09/1211312:21 09/18/1321:24 1
AlooM DOwdon %Recovey Qual1Mr LANb Pipared Analyzed Da rac
13C-1,$3,4,7,8.HxCDF 55 40-135 09/121i312:21 09/18�1321:24 1
13C-1,2,3,6,7,8-HxCDD 60 40-135 0911211312:21 09/la/1321:24 1
13C-2,3,7,8-TCDD 60 40-135 09/1V1312.21 091IN1321:24 1
13C-1,2,3,7,8-PeCDD 62 40-135 091121131221 09118/13 21:24 1
13C-1,23,4,6,7,8-HpCDF 62 40-135 0911211312:21 09//811321:24 1
13C-1,2,3,7,8-PeCDF 58 40-135 0911211312.21 0911&1321:24 /
13C-2,3,7,8-TCDF 57 40-135 09/12/1312:21 0911811321:24 1
13C-2,3,7,8-TCDF 62 40-135 09/12/13 ffi21 0920//302.30 1
13C-1,23,4,6,7,8-HpCDD 64 40-135 09/12/1311-21 09H8/1321:24 1
13C-WDD 67 40-135 0911V1312.21 0911W321:24 /
General Chemistry
Am" Result QuallRer NONE NONE Unit D Prepared Analyzed Dil Fac
Pemern Lipids 8.7 % M12113 12:21 09/13/13 11:07 1
Client Sample ID: SITE 48 COMMON CARP WEI Lab Sample ID: 320-3948-7
Date Collected:08/06/13 13:10 Matrix:Tissue
Date Received:09/06113 08:15
Method:8290-Dioxins and Furans(HRGC/HRMS)
Am" Rewa Qualltbr RL TOL unk D Prepared Anai Oil Fac
OCDO 58 H 9.7 4.8 py9 09/12/13 12:21 OW18113 22:07 1
Total POWD ND H 4.8 2.4 P9/9 09/12/1312:21 09/18/1322:07 1
1,2,3,7,8,9-HxCDD ND H 4.8 2.4 p9/9 09/12/1312:21 09/1811322:07 1
1,2,3A,7,8,9-HpCDF ND H 4.8 2.4 p9/9 09/12/1312:21 09/18/1322:07 1
1,2,3,4,6,7,8-HpCDF ND H 4.8 2.4 p9/9 09/1211312:21 09/18/1322:07 1
Total TCDF 7.1 H 0.97 0.48 pale 09/12/1312.21 09/1811322:07 1
TestAmerica Sacramento
Page 8 of 22 1/30/2014

Client Sample Results
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID 320-3948-1
ProjectlSite:Tissue
Client Sample ID: SITE 4B COMMON CARP WB Lab Sample ID: 320-3948-7
Date Collected:08/06/13 13:10 Matrix: Tissue
Date Received:09/05/13 08:15
Method:8290-Dioxins and Furans(HRGC/HRMS)(Continued)
Analyte Result Oualxier ILL TDL Unit D Prepared Analyzed Dil Fac
Total HxCDF NO H 4.8 2.4 p9/9 M1211312:21 09/18/1322.07 1
1,2,3.7,8-PeCDD NO H 4.8 2.4 p9/9 09/1211312:21 0911811322:07 1
1,2,3,7,8-PeCDF ND H 4.8 2.4 pg/g 09112/131221 09118/1322:07 1
1,2,3,6,7,8-HxCDD 4.9 H 4.8 2.4 p9/9 09/12/13 12:21 09/18/13 22:07 1
1,2,3,6,7,8-HxCDF ND H 4.8 2.4 p9/9 09112/1312:21 09/1811322:07 1
Total TCDD 2.4 H 0.97 OA8 p9/9 09/12/13 12:21 09/18/13 22:07 1
1,2,3,7,8,9-HxCOF ND H 4.8 2A pg/9 09/1211312:21 09/1811322:07 1
OCDF NO H 9.7 4.8 p9/9 09/1211312*21 09118/1322:07 1
Total PeCDF 3.8 J H 4.8 2.4 p9/9 09/12/13 12:21 09/18/13 22:07 1
Total HpCDD 15 H 4.8 2.4 P9/9 09112/1312:21 0911811322:07 1
1,2,3,4,7,8-HxCDD ND H 4.8 2.4 p9/9 0911211312:21 09/1a/1322:07 1
Total HxCDD 4.9 H 4.8 2.4 Pyg 09/12/13 12:21 09/18/13 22:07 1
Total HPCDF ND H 4.8 2.4 p9/9 09112J1312:21 09/18/13 22:07 1
1,2,3,4,7,8-HXCDF ND H 4.8 2.4 p910 09/12/13 12:21 09/18/13 22:07 1
1,2,3,4,6,7,8-HpCDD 15 H 4.8 2A pole 09/1211312:21 0911811322:07 1
2,3,4,6,7,5.HxCDF NO H 4.8 2.4 polo 09/1211312:21 09/1811322:07 1
2,3,7,8-TCDD 2.4 H 0,97 0.48 p9l9 09/12/13 12:21 09/18/13 22:07 1
2,3,7,8-TCDF 5.8 H Os7 0.48 polo 09/12/13 12:21 09/20/13 03:08 1
2,3,4,7,8-PeCDF ND H 4.8 2.4 py9 09/12/13'12:21 0911811322:07 1
/aotope D1ludon %RaCowry Ow118er Limas Prepared Analyzed Dd Fac
13C-1,2,3,4,7,8-HxCDF 58 40-135 0911211312.21 09N8/1322,07 1
/3C-1,2,3,8,7,8-HxCDD 60 40-135 0911211312.21 09/1811322:07 1
13C-23,7,8-TCDD 59 40-135 OM211312.21 09/1W322.07 /
13C-1,23,7,8-PeCOD 62 40-135 0911211312.21 09/la'1322:07 1
13C-1,2,3,4,6,7.8-HpCDF 63 40-135 0911211312.21 09/18/1322.07 1
13C-1,23,7,8-PeCDF 57 40-135 0911211312:21 09/18n322:07 1
13C-2,3,7,8-TCDF 56 40-135 091121131t21 09//a/1322:07 1
13C-2,3,78-TCDF 60 40-135 09/1211312:21 0920/1303.as 1
13C-1,2,3,4,6,78-HxCDD 65 40-135 08/12(1312:21 09/18132207 /
L 13C-OCDD 71 40-135 09/12/13 12:21 09//a/13 22:07 1
General Chemistry
And" Resun Oualifier NONE NONE Unit D Prepared An@WW Dil Fac
Percent Lipids 15 % OW12/13 12.21 O9/13/13 11:07 1
Client Sample ID: COMPOSITE OF 4B CHANNEL CAT#3,5 Lab Sample ID: 320-3948-8
Date Collected:08/06/13 00:00 Matrix:Tissue
Data Received:09/05113 08:15
Method:8290-Dioxins and Furans(HRGC/HRMS)
Ana" Result OualBfer RL TDL Unx D Prepared Analyzed Dil Fac
OCDD NO H 9.9 4.9 p9/9 09M2/1312:21 09/18/1322:51 1
Total PeCDD NO H 4.9 2.5 p9/9 0911211312:21 09/1811322:51 1
1,2.3,7,8,9-HxCDD ND H 4s 25 p9/9 09/1211312:21 OW18/1322:51 1
1,2,3,4,7,8,9-HpCDF ND H 4.9 2.5 pgig 09/1211312:21 09/18/1322:51 1
1,2,3,4,6,7,8-HpCDF ND H 4.9 2.5 pg/g 09/12/13 1221 09/18/1322:51 1
Total TCDF ND H 0.99 0.49 pg/g 09/12/13 12:21 09/18/1322:51 1
Total HxCDF ND H 4.9 2.5 pg/g 09/12/13 12:21 09/18/13 22:51 1
1.2,3,7,E-PeCDD ND H 4.9 2.5 p9/g 0911211312:21 09/1811322:51 1
1,2,3,7,8-PeCDF ND H 49 2.5 p9/9 09/12/13 12:21 09/18/13 22:51 1
TestAmerica Sacramento
Page 9 of 22 1/30/2014

Client Sample Results
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID: 320-3948-1
Project/Site:Tissue
Client Sample ID: COMPOSITE OF 4B CHANNEL CAT #3,5 Lab Sample ID: 320-3948-8
Date Collected: 08106113 00:00 Matrix:Tissue
Date Received:09/05/13 08:15
Method:8290-Dioxins and Furans(HRGC/HRMS)(Continued)
AR*W Reaua Qualifier RL TDL Unit D Prepared Analyzed Oil Fac
1,2,3,6,7,&HxCDD NO H 4.9 2.5 pg/9 09/1211312:21 0911611322:51 1
1,2,3,6,7,8-HxCDF NO H 4.9 2.5 P9/9 09/1211312:21 09/la/1322:51 1
Total TCDD NO H 0.99 0.49 pg/g 09/12/1312:21 09/18/1322:51 1
1,2,3,7,8s-HxCDF NO H 4.9 2.5 pg/g 09/12/1312:21 09118/1322:51 1
OCDF ND H 9.9 4.9 pg/g 09/12/1312:21 09/18/1322:51 1
Total PeCDF NO H 4.9 2.5 pg/g 09/12/1312:21 09118/1322:51 1
Total HPCDD ND H 4.9 2.5 pg/g 09/1211312:21 09118/1322:51 1
1,2.3,4,7,&HxCDD NO H 4.9 2.5 pg/9 09/12/1312:21 09/18/1322:51 1
Total HxCDD NO H 4.9 2.5 pg/g 09/12/1312:21 09/18/1322:51 1
Total HpCDF NO H 4.9 2.5 pg/g 0911211312:21 09/18/1322:51 1
1,2,3,4,7,8-HxCDF NO H 4.9 2.5 p9/9 09112J1312:21 09/18/1322:51 1
1,2,3,4,6,7,8-HPCDD NO H 4.9 2.5 P9/9 09/1211312:21 09fi8/1322:51 1
2,3,4,6,7,8-HxCDF NO H 4A 2.5 p9/9 09/12/1312:21 09/18/1322:51 1
2,3.7,8-TCDD NO H 0-99 0A9 p9/9 09/12/1312:21 09/1811322:51 1
2,3,7,8-TCDF NO H 0.99 049 p9/9 09/12/1312:21 09118/1322:51 1
2,3,4,7,8-PeCOF NO H 4.9 2.5 pg/9 09/12/1312:21 0911811322:51 1
bo/ope Davison %Recovery Owllnar L6N# Prpwed Analyzed Da Fat
13C-1,2,3,4,7,&HxCDF 62 40-135 09/1Yf312:21 69H8H322:61 1
/3C-1,2,3,6,7,8-HXCDO 64 40-135 0911211312:21 Mas,1322:51 1
13C-2.3,7,8-TCDD 66 40-135 0911211312:21 09H811322:51 1
13C-1,2,3,7,8-PeCDD 69 40-135 0911211312.21 09/18n1322:51 1
13G7,23,4,6,7,8a1pCDF 72 40-135 091121'1312:21 OWlIV1322:51 1
13C-1,2,3,7,8-PeCDF 64 40-135 0911211312.21 0911W322:51 1
13C-2,3,7,8-TCDF 62 40-135 09/12/13/2.21 09/18/1322:51 1
13C-1,2,3,4,Q7,8HPCDD 75 40-135 091121131221 0911&1322.51 1
13C-OCDO 80 40-135 09112113 12.21 COMM 22.51 1
CGeneral Chemistry
An*W Result Qualifier NONE NONE Unit D Prepared Analyzed Oil Fa:
Parted Llpids 2.7 i 09/12/1312:21 09/13/1311:07 1
Client Sample ID: COMPOSITE OF 4B COMMON CARP #4,6 Lab Sample ID: 320-3948-9
Date Collected:08106/13 00:00 Matrix: Tissue
Date Received:09/05/13 08:15
Method:8290-Dioxins and Furarts(HRGCIHRMS)
Analyte Result Qualifier RL TDL Una D Prepared Analyzed oil Fac
OCDD 14 H 9.8 4.9 Pg/g 09/1211312:21 OW1811323:34 1
Total PeCDD NO H 4.9 2.6 p91g 09/12/1312:21 09/18/1323:34 1
1,2.3,7,8,9-HxCDD NO H 4.9 2.6 pg/9 09/1211312:21 09118/1323:34 1
/,2,3,4,7,8,9.HpCDF NO H 4.9 2.5 119/9 0911211312:21 0911811323:34 1
1,2,3,4,6,7,8-HpCDF ND H 4.9 2.6 P9/9 09/12/1312:21 09/1811323:34 1
Total TCDF 1.7 H 0,98 0.49 pg/p 09/1211312:21 0911811323:34 1
TOW HxCDF NO H 4.9 2.6 pg/9 09/12/1312:21 0911811323:34 1
1,2,3,7,&PeCDD NO H 4.9 2.6 pg/g 09/1211312:21 09/18/1323:34 1
1,2,3,7.8-PeCDF NO H 4s 2.5 PW9 09/12/1312:21 09118/1323:34 1
1,2.3,6,7,8-HxCDD ND H 4.9 2.5 Pg/9 09/1211312:21 09/18/1323:34 1
1,2,3,6,7,8-HxCDF NO H 4.9 2.5 pg/g 09/12/13 12:21 09/18/13 23:34 1
Total TCDD 0.70 J H 0.98 0.49 pg/g 09/12/1312:21 09/18/13 23:34 1
1,2,3,7,8,9-HxCDF NO H 4.9 2.5 p9/9 09/12/'1312:21 09118/1323:34 1
TestAmerica Sacramento
Page 10 of 22 1/30/2014

Client Sample Results
Client: Evergreen Packageing FKABlue Ridge Paper TestAmenca Job ID. 320-3948-1
Project/Site:Tissue
Client Sample ID: COMPOSITE OF 4B COMMON CARP #4,6 Lab Sample ID: 320-3948-9
Date Collected: 08/06/13 00:00 Matrix:Tissue
Date Received:09106113 08:15
Method:8290•Dioxins and Furans(HRGCIHRMS)(Continued)
Am" Resuit QualMer RL TDL unit D Preparod Analyzed Oil Fad
OCDF ND H 9.8 4.9 pg/g 09/12/1312:21 09/18/1323:34 1
Total PeCDF ND H 4.9 2.5 pg/g D9/1 211 3 12:21 09118/1323:34 1
Total HpCDD 4.9 H 4.9 2.5 Pg/g 09/12/1312:21 09118/1323:34 1
1,2,3.4,7.8-HxCDD ND H 4.9 2.5 139/9 OW1211312:21 09118/1323:34 1
Total HxCDD NO H 4.9 2.5 Pg/g OW1211312:21 09118/1323:34 1
Toted HpCDF ND H 4.9 2.6 pg/g 09/12/1312:21 09118/1323:34 1
1,2,3,4,7,8-HxCDF NO H 4.9 2.5 p9/9 09/1211312:21 09118/1323:34 1
1,2,3,4,6,7,8-HpCDD 4.9 H 4.9 2.5 p9/9 09/1211312:21 09/1811323:34 1
2,3,4,6,7,8-HxCDF NO H 4.9 2.5 pg/g 09/1211312:21 09118/1323:34 1
2,3,7,8-TCDD 0.70 JH 0.98 0,49 pg/g 09/1211312:21 0911811323:34 1
2,3,7,8-TCDF 1.4 H 0.98 0.49 P9/9 09/1211312:21 09/20/1303:47 1
2,3,4,7,8-PeCDF NO H 4.9 2.5 p9/9 09/1211312:21 0911811323:34 1
1ao11 Dfiutlml %Recovery Que189er LerdO Prepaved Andynd DN Fad
13C-1,23,4,7,8-HxCDF 58 40-135 0911211312:21 091IM323.34 1
13C-1,2,3,6,7,8-HxCDD 60 40-135 09nW1312.21 OWIW1323.34 1
13C-2,3,7,8-TCDD 64 40-135 09/1271312:21 09/1a/1323.34 1
13C-1,2,3,7,8-PeCDO 67 40-135 08/1211312:2i 09118//323.34 1
13C-1,2,3,4,6,7,8-HpCDF 63 40-135 0911211312.21 0,M&1323.34 /
13C-1,$3,7,8-PeCOF 63 40-135 091120312.21 09118N323.34 /
13C-2,3,7,8-TCDF 61 40-135 09112113 12:21 09118113 23.34 1
1302,3,7.8-TCDF 65 40-135 0911211312:21 0912W303.47 /
13C-1,2,3,4,6,7,8-HpCDD 67 40-135 0911211312.21 09/1ar1323.34 /
13C-OCDD 72 40-135 OWIV1312.21 0911ar1323.34 1
General Chemistry
Analrb Result Qualifier NONE NONE unit D Pregnd Analyzed oil Fac
Percent uplds 4.2 % 09/12/1312:21 09/1311311:07 1
TestAmerica Sacramento
Page 11 of 22 1/30/2014

Isotope Dilution Summary ❑
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID:320-3948-1 1
ProjectlSite:Tissue 12
L.
— I
Method: 8290-Dioxins and Furans (HRGC/HRMS)
Matrix:Tissue Prep Type:Total/NA
Percent Isotope Dilution Recovery(Acceptance Limits) L
HxCDF1 HxCDD2 TCDD PeCDD HpCDF1 PeC0F1 TCDF HpCDD
Lab Sample ID Client Sample ID (40.135) (40435) (40435) (40.135) (40435) (40435) (40-135) (40.135)
320-3948-1 SITE 4A CHANNEL CAT R-FILLET: 62 68 66 69 66 63 64 68
320-3948-2 SITE 4A COMMON CARP 55 60 60 62 62 58 57 64 °
R-FILLETS
320.3948-2 SITE 4A COMMON CARP 62
R-FILLETS ❑
320J946-7 SITE 46 COMMON CARP NB 56 60 59 62 63 57 56 65
32D3948-7 SITE 4B COMMON CARP WB 60
3203948-8 COMPOSITE OF 4B CHANNEL 62 64 66 69 72 64 62 75 (1
CAT#3,5 C'1
3203948-9 COMPOSITE OF 4B COMMON 58 60 64 67 63 63 61 67
CARP 94,6 i
3203948-9 COMPOSITE OF 4B COMMON 65 _
CARP #4,6 I
LCS 320.25010/2-A Lab ConWl Sample 69 68 87 68 72 64 65 72
MB 320-2501011-A Method Blank 58 58 59 60 62 56 57 64
Percent Isotope Dilution Recovery(Acceptance Limits)
OCDD !t
Lab Sample ID Client Sample ID (40.135)
320-3948-1 SITE 4A CHANNEL CAT R-FILLET: 72
3203948-2 SITE 4A COMMON CARP 67
R-FILLETS I _�
320-3948-2 SITE 4A COMMON CARP
R-FILLETS
320.3948-7 SITE 4B COMMON CARP WB 71
3203948-7 SITE 4B COMMON CARP WB
3203948-8 COMPOSITE OF 4B CHANNEL 80
CAT#3,5
3203948-9 COMPOSITE OF 48 COMMON 72
CARP #4,6
32D3948-9 COMPOSITE OF 4B COMMON
CARP #4,6
LCS 320-25010/2-A Lab Control Sample 74
MB 320-25010/1-A Method Blank 69
Surrogate Legend
HxCDF1=13C-1,2,3,4,7,8-HxCDF
HxCDD2=13C-1,2,3,6,7,8-HpCDD
TCDD=13C-2,3,7,8-TCOD
PeCDD=13C-1,2,3,7,8-PeCDD
HpCDF1=13G7,2,3,4,6,7,8-HpCDF
PeCDF1=13C-1,2,3,7,8-PeCDF
TCDF=13C-2,3,7,8-TCDF
HpCDD=13C-1,2,3,4,6,7,8-HpCDD
OCDD=13C-OCDD
TestAmerica Sacramento
Page 12 of 22 1/30/2014

QC Sample Results
Client Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID:320-3948-1
Project/Site:Tissue
Method: 8290-Dioxins and Furans(HRGC/HRMS)
F Lab Sample ID: MB 320-2501011-A Client Sample ID: Method Blank
Matrix: Tissue Prep Type:TotaUNA
Analysis Batch: 25486 Prep Batch: 25010
MB Me
Analyte Result Owlkir RL TDL Unit D prepared Analyzed Dil Fac
OCDD ND 10 5.0 pwg 09/1211312:21 09/18/1319:15 1
Total PeCDD ND 5.0 2.5 P9/9 09/12/1312:21 09/18/1319:16 1
1,2,3,7,8,9-HxCDD ND 5.0 2.5 p9/9 09/12/13 12:21 09/1 all 19:15 1
1,2,3,4,7,8,9-HpCDF ND 5.0 2.5 p9/9 09/12/13 12:21 09/18/13 19:15 1
1,2,3,4,6,7,8-HpCDF ND 5.0 2.5 P9/9 09/12/13 12:21 09/18/13 19:15 1
Total TCDF ND 1.0 0.50 p9/9 09/12/13 12:21 09/18/13 19:15 1
Total H%CDF ND 5.0 2.5 P9/9 0911211312:21 09/18/1319:15 1
1,2,3,7,8-PeCDO ND 5.0 2.5 p9/9 0911211312:21 09/18/1319:15 1
1,2,3,7,8-PeCDF ND 5.0 2.5 P9/9 0911211312:21 09/18/13 19:15 1
1,2,3,6,7,8-HxCDD ND 5.0 Is pyg 09/12/13 12:21 09/1 all 19:15 1
1,2,3,6,7,8-HxCDF ND 5.0 2.5 pg/g 0911211312:21 09/18/13 19:15 1
Total TCDD ND 1.0 0.50 pg/g 09/12/1312:21 09/18/13 19:15 1
1,2,3,7,8,9-HxCDF ND 5.0 2.5 pyg 0911211312:21 09118/1319:15 1
OCDF ND 10 5.0 p9/g 0911211312:21 09/18/1319:15 1
Total PeCDF ND &0 2.5 Pg/g 0911211312:21 09/18/13 19:15 1
Total HpCDD ND 5.0 2.5 P9/9 09/12/1312:21 09/18/1319:15 1
1,2,3,4,7,8-HxCDD ND 5.0 2.5 p9/9 09/12/13 12:21 09/18/13 19:15 1
Total HxCDD ND 5.0 2.5 P9/9 09/1211312:21 09/18/13 19:15 1
Total HPCDF ND 5.0 2.5 p9/9 09/1211312:21 09/18/13 19:15 1
1,2,3,4,7,8-HxCDF ND 5.0 25 Pg/g 09/12/13 12:21 09/18/13 19:15 1
1,2,3,4,6,7,8-HpCDD ND 5.0 2.5 pg/g 09/12/13 12:21 09/11IM3 19:15 1
2,3,4,6,7,8-HxCDF ND 5.0 2.5 P9/9 09/12/13 12:21 09/18/13 19:15 1
2,3,7,8-TCDD ND 1.0 0.50 pg/g 09/12/13 12:21 09/18/13 19:15 1
2,3,7,8-TCDF ND 1.0 0.60 pg/g 09112/1312:21 0911811319:15 1
2,3,4,7,8-PeCDF ND 5.0 2.5 p9/9 09/12/13 12:21 09/18/13 19:15 1
MB Me
bo"DiiUdon %Recovery OwIMr Limns Frepawd Ana/yxd DB Fac
13C-1,2,3,4,7,&HxCDF 58 40-135 08/fYi912:21 09/i&1319:15 1
13C-1,2,3,8,7,8-HxCDD 58 40-135 0911211312.21 09M8/1319:15 1
13C-2,3,7,8-TCDD 59 40-135 09112/1312.21 0911410319.15 1
13C-1,2,3,7,8-PeCDD 60 40-135 0911211312.21 091180319.15 1
13C-1,2,346,7,8-HpCDF 62 40-135 09112J1312.21 0911S/1319:15 1
13C-1,237,8-PeCDF 56 40-135 0911211312.21 09118H319:16 1
13C-2,3,7,8-TCDF 57 40-135 0911211312.21 09/18/1319:15 1
13C-1,$34,6,7,8-HPCDD 64 40-135 09/12/1312.21 0911811319:/5 1
13C-OCDD 69 40-135 09/12/13 12.21 09/18/13 19.15 1
Lab Sample ID: LCS 320-2501012-A Client Sample ID:Lab Control Sample
Matrix:Tissue Prep Type:Total/NA
Analysis Batch:25486 Prep Batch:25010
Spike LCS LCS %Rec.
Analyte Added Result Qualifier Unit D %Rec Lknits
OCDD 200 208 Pg/g 104 50-160
1,2,3,7,8,9-HxCDD 100 105 pg/g 105 50-150
1,2,3,4,7,8,9-HpCDF 100 98.0 p9/9 98 50-150
1,2,3.4,6,7,8-1-lpCDF 100 106 P9/9 106 50-150
1,2,3,7,8-PeCDD 100 98.8 P9/9 99 50-150
1,2,3,7,&PeCDF 100 105 pg/g 105 50-150
TestAmerica Sacramento
Page 13 of 22 1/30/2014

QC Sample Results
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID: 320-3948-1
Project/Site:Tissue
Method: 8290-Dioxins and Furans(HRGC/HRMS)(Continued)
Lab Sample ID: LCS 320-25010/2-A Client Sample ID: Lab Control Sample
Matrix: Tissue Prep Type: Total/NA
Analysis Batch: 25486 Prep Batch: 25010
Spike LCS LCS %Rec.
Analyre Added Reeuit Qualifier Unit D %Rec Limits
1,2,3,6,7,8-HxCDD 100 106 pg/9 106 50-150
1,2,3,6,7,8-HxCDF 100 118 p9/9 118 50-150
1,2,3,7,8,9-HxCDF 100 108 pg/g 108 50-150
OCDF 200 197 pg/g 99 50-150
1,2.3,4,7,8-HXCDD 100 110 pg/g 110 50-150 e
1,2.3.4,7,8-HxCDF 100 111 pg/g 111 50-150
1.2.3,4,8,7,8-HpCDD 100 106 pg/g 106 50-150
2,3,4,6,7,8-HxCDF 100 114 pg/g 114 50-150
2,3,7,8-TCDD 20.0 20.4 p9/9 102 50-150
2,3,7,8-TCDF 20.0 230 pg/g 115 50.150
2,3,4,7,8-PeCDF 100 102 pg/g 102 50-150
LCS LCS
ktohipe Dilution %Recovery Qualifier Lhnits
13C-1,2,3,4,7,8-HxCDF 69 40-135
13C-1,2,3,8,7,8-HxCDD 68 40.135 =
13C-2,3.7,8-TCDD 67 40-135 " f"
13C-1,2,3,7,8-PeCDD 68 40-135
13C-1.2,3,4,6,7,8-HxCDF 72 40-135
13C-1,2,3,7,8-PeCDF 64 40.135
13C-2,3,7,8-TCOF 65 40.135
13C-1,2,3,4,8,7,6-HpCDD 72 40-135
13C-OCDD 74 40-135
TestAmerica Sacramento
Page 14 of 22 1/30/2014

QC Association Summary
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID 320-3948-1
Project/Site:Tissue
Specialty Organics
Prep Batch:26010
Lab Sample ID C 1wit Sample ID Prep Type Matrix Method Prep Batch
320-3948-1 SITE 4A CHANNEL CAT R-FILLETS Total/NA Tissue 8290
320-3948-2 SITE 4A COMMON CARP R-FILLETS Total/NA Tissue 8290
320-3948-7 SITE 4B COMMON CARP WS Total/NA Tissue 8290
320-3948-8 COMPOSITE OF 48 CHANNEL CAT#3,5 Total/NA Tissue 8290
320-3948-9 COMPOSITE OF 4B COMMON CARP 94,6 Total/NA Tissue B290
LCS 320-2501012-A Lab Control Sample Total/NA Tissue 8290
MB 320-25010/1-A Method Blank Total/NA Tissue 8290
Analysis Batch:25486
Lab Sample ID Client Sample to Prep Type Matrix Method Prep Bach
3203948-1 SITE 4A CHANNEL CAT R-FILLETS TOlel/NA Tissue 8290 25010
320-3948-2 SITE 4A COMMON CARP R-FILLETS Total/NA Tissue 8290 25010
320-3948-7 SITE 4B COMMON CARP We Total/NA Tissue 8290 25010
320-394" COMPOSITE OF 4B CHANNEL CAT#3,5 Total/NA Tissue 8290 25010
320-3948-9 COMPOSITE OF 4B COMMON CARP #4,6 Total/NA Tissue 8290 26010
LCS 32 0-2 6 01 0/2-A Lab Control Sample Total/NA Tissue 8290 25010
MB 320-25010/1-A Method Blank Total/NA Tissue 8290 25010
Analysis Batch:25602
Lab Sample ID CINm Sample ID Prep Type Matrix Method Prop Bach
320-3948-2 SITE 4A COMMON CARP R-FILLETS Total/NA Tissue 8290 25010
320-3948-7 SITE 48 COMMON CARP WB Total/NA Tissue B290 25010
320-3948-9 COMPOSITE OF 413 COMMON CARP $4.6 Total/NA Timm 8290 25010
General Chemistry
Prep Batch:25092
Lab Sasipla ID CBatt Sample ID Prep Type Ma di, Method Pnp Batch
320-3948-1 SITE 4A CHANNEL CAT R-FILLETS Total/NA Tissue HRMS-Sox
320-3948-2 SITE 4A COMMON CARP R-FILLETS Total/NA Tissue HRMS-Sox
320-3948.7 SITE 4B COMMON CARP WS Total/NA Tissue HRMS-Sox
320-3948-8 COMPOSITE OF 48 CHANNEL CAT#3,5 Total/NA Tissue HRMS-Sox
320-3948-9 COMPOSITE OF 4B COMMON CARP #4,6 Total/NA Tissue HRMS-Sox
Analysis Batch:25097
Lab Sample 10 Clan Sample ID Prep Type Mob Method Prep Balch
3203948-1 SITE 4A CHANNEL CAT R-FILLETS TOtal/NA Tissue Lipids 25092
320-3948-2 SITE 4A COMMON CARP R-FILLETS Total/NA Tissue Lipids 25092
320-3948-7 SITE 4B COMMON CARP WS Total/NA Tissue Lipids 25092
320-3948-8 COMPOSITE OF 4B CHANNEL CAT#3,5 Total/NA Tissue Lipids 25092
320-3948-9 COMPOSITE OF 48 COMMON CARP #4,6 Total/NA Tissue Lipids 25092
TestAmerica Sacramento
Page 15 of 22 1/30/2014

Lab Chronicle
Client Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID:320-3948-1 -i
Projectl5ite:Tissue
L?
r 7
Client Sample ID: SITE 4A CHANNEL CAT R-FILLETS Lab Sample ID: 320-3948-1
Date Collected:0 810 6113 14:00 Matrix:Tissue
Date Received:09/05/13 08:16
Batch Batch oil Initial Final Batch Prepared
Prep Type , Type Method Run Factor Amount Amount Number or Analyzed Analyst Lab
TOtallNA Prep 8290 10.44 g 20 uL 25010 09/12/13 12:21 GOB TAL SAC
e
TOtat1NA Analysis 8290 1 10.44 g 20 uL 25486 09/18l13 20:41 ALM TAL SAC
TotaVNA Prep HRMSSox 10.44g 10.44g 25092 09112/1312:21 AVM TALSAC
TotallNA Analysis Lipids 1 10.44g 10.44g 25097 0911311311:07 GOB TALSAC
Client Sample ID: SITE 4A COMMON CARP R-FILLETS Lab Sample ID: 320-3948-2 ❑
Date Collected:0 810511 3 1 6:20 Matrix:Tissue o
Date Received:09105113 08:16 m
Batch Batch Oil Initial Final Batch Prepared
Prep T Type Method Run Factor Amount Amount. Number or Analyzed Analyst Lab r t
Total/NA a Prep 8290 10.22 g 20 uL 25010 09/12/13 12 21 GOB TALSAC I
Total/NA Analysis 8290 1 10.22 g 20 uL 25486 09/18/13 21:24 ALM TAL SAC
Total/NA Prep 8290 10.22 g 20 uL 25010 09/1211312:21 GOB TAL SAC �—
Total/NA Analysis 8290 1 10.22 g 20 uL 25602 09/20/13 02:30 LLH TALSAC
Total/NA Prep HRMS-Sox 10.22 g 10.22 g 25092 09/12/1312:21 AVM TALSAC
TotagNA Analysis Lipids 1 10.22 g 10.22 g 25097 09/13/13 11:07 GOB TALSAC i I I'
Client Sample ID: SITE 413 COMMON CARP WB Lab Sample ID: 320-3948-7
Date Collected:0 810 611 3 13:10 Matrix:Tissue
Date Received:09/06113 08:15
Batch Batch oil Initial Final Batch Prepared
Prep Type Type Method Run Factor Amount Amount Number or Analyzed Analyst Lab
Total/NA Prep 8290 10.36 g 20 uL 25010 09/12/13 12:21 GOB TALSAC
TOta1/NA Analysis 8290 1 10.36g 20 uL 25486 0911811322:07 ALM TALSAC
Total/NA Prep 8290 10.36 g 20 uL 25010 09/12/13 12:21 GOB TALSAC
TOtel/NA Analysis 8290 1 10.36 g 20 uL 25602 09/20/13 03:08 LLH TAL SAC
TotagNA Prep HRMS-Sox 10.36g 10.36g 25092 09/1211312:21 AVM TALSAC
Total/NA Analysis Lipids 1 10.36 g 10.36 g 25097 09/13/13 11:07 GOB TALSAC
Client Sample ID: COMPOSITE OF 4B CHANNEL CAT#3,5 Lab Sample ID: 320-3948-8
Date Collected:08106/13 00:00 Matrix:Tissue
Date Received:09/05/13 08:16
Batch Batch DII Initial Final Batch Prepared
Prep Type Type Method Run Factor Amount Amount Number or Analyzed Analyst Lab
TotaVNA Prep 8290 10.15 g 20 uL 25D10 09/1211312:21 GOB TALSAC
Total/NA Analysis 8290 1 10.15 g 20 uL 25486 09/18/13 22:51 ALM TALSAC
TotaVNA Prep HRMS-Sox 10.15 g 10.15 g 25092 09/12/13 12:21 AVM TAL SAC
TotagNA Analysis Lipids 1 10.15 g 10.15 g 25097 09/13/13 11:07 GOB TAL SAC
TestAmerica Sacramento
Page 16 of 22 V30I2014

Lab Chronicle M
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID:320-3948-1
Project/Site:Tissue [
Client Sample ID: COMPOSITE OF 4B COMMON CARP #4,6 Lab Sample lb: 320-3948-9 C1
Date Collected:0810 6113 0 0:00 Matrix:Tissue
Date Received:09/05/13 08:16 1-61
Batch Batch DII Initial Final Batch Prepared
Prep Type Type Method Run Factor Amount Amount Number or Analyzed Analyst Lab
TotalfNA Prep 8290 10.16 g 20 uL 25010 09/12/13 12:21 GDB TAL SAC
TotaUNA Analysis 8290 1 10.16 g 20 uL 25486 09/18/13 23:34 ALM TAL SAC
Total/NA Prep 8290 10.16 g 20 uL 25010 09/12/13 12:21 GDB TAL SAC
Tmal/NA Analysis 8290 1 10.16 g 20 uL 25602 09/20/13 03:47 LLH TAL SAC
TotaUNA Prep HRMS-Sox 10.16 g 10.16 g 25092 0911211312:21 AVM TAL SAC
Total/NA Analysis Lipids 1 10.16 g 10.16 g 25097 09/13/13 11:07 GDB TAL SAC
Laboratory References:
0
TAL SAC=TestAmerica Sacramento,B80 Riverside Parkway,West Sacramento,CA 95605,TEL[phone] m
f d,
0
0
TestAmerica Sacramento
Page 17 of 22 1/30/2014

Certification Summary �]
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID:320-3948-1
Project/Site:Tissue
Laboratory: TestAmerica Sacramento J�
All certifications held by this laboratory are listed. Not all certifications are applicable to this report
/I
Authority Program EPA Region Certification ID Expiration Date 7
A21-A A21-A NE-OS-22-13 01-31-14
A21-A DoD ELAP 2928-01 03-31-14
Alaska(UST) State Program 10 UST-055 02-03-14' ❑
Arizona State Program 9 AZ0708 08-11-14
Arkansas DEQ State Program 6 88-0691 06-17-14
California NELAP 9 1119CA 01-31-14
Colorado State 8 N/A 08-31-14 FV
Connecticut State Program 1 PH-0691 06-30-15
Florida NELAP 4 E87570 06-30-14 101
Guam State Program 9 N/A 08-31-14 (1
Hawaii Stale Program 9 N/A 01-31-14' �'
Illinois NELAP 5 200060 03-17-15 1
Kansas NELAP 7 - E-10375 10-31-14
Louisiana NELAP 6 30612 06-30-14 m
Michigan State Program 5 9947 01-31-14
Nebraska State Program 7 NE-OS-22-13 01-31-14 [
Nevada State Program 9 CA44 07-31-14 L�_
New Jersey NELAP 2 CA0g5 06-30-14
New York NELAP 2 11666 03-31-14
Northern Mariana Islands State Program 9 MP0007 02-01-14
Pennsylvania NELAP 3 68-01272 03-31-14 1I��
South Carolina State Program 4 87014 06-30-14
Texas NELAP 6 T104704399-08-TX 05-31-14 1�4
US Fish&Wildlife Federal LE148388-0 12-31-14
USDA Federal P330-11-00436 12-30-14
USEPA UCMR Federal 1 CA00044 11-06-14
Utah NELAP 8 QUAN1 01-31-14
Washington State Program 10 C581 05-05-14
West Virginia DHHR State Program 3 9930C 02-03-14'
Wyoming State Program 8 8TMS-0 01-31-14
•Expired certification is currently pending renewal and is considered valid.
TestAmerica Sacramento
Page 18 of 22 1/30/2014

Method Summary
Client Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID:320-3,948-1
r Project/Site:Tissue
.Method Method Description _ Protocol Laboratory
8290 Dioxins and Furans(HRGC/HRMS) SW846 TALSAC
Lipids Percent Lipids TestAmerica SOP TAL SAC -"
Protocol References:
SW846="Test Methods For Evaluating Solid Waste,Physical/Chemical Methods',Third Edition,November 1986 And Its Updates.
TestAmedca SOP=TestAmerica,Inc., Standard Operating Procedure r
Laboratory References:
TAL SAC=TestAmerica Sacramento,880 Riverside Parkway,West Sacramento,CA 95605,TEL[phone]
0
�o
i . .t
c H�
1 Li] A
TestAmerica Sacramento
Page 19 of 22 1/30/2014

Sample Summary
Client: Evergreen Packageing FKABlue Ridge Paper TestAmerica Job ID:320-3948-1
Project/Site:Tissue
Lab Sample ID Client Sample ID Matrix Collected Received
320-3948-1 SITE 4A CHANNEL CAT R-FILLETS Tissue 6810511 114:00 09/05/1 008:15 �1
320-3948-2 SITE 4A COMMON CARP R-FILLETS Tissue 0 810 5113 16:20 09/05/13 08:15
320-3948-7 SITE 48 COMMON CARP WB Tissue 0 8/0 6113 13:10 09/05/13 08:15
,320-3948-8 COMPOSITE OF 4B CHANNEL CAT#3,5 Tissue 08/0611300:00 09/0511308:15
320-3948-9 COMPOSITE OF 4B COMMON CARP #4,6 Tissue 08/06/13 00:00 09/05/13 08:15
e
a
0
� a
I
TestAmerica Sacramento
Page 20 of 22 1/30/2014

TestAmerlca Sacramento Chain of Custody Record n �pp1 Test erica
88D Riverside Pad:Way 1I1p1Ip1t1 II1IIIIf1 11p11 I11,II1II11�I�I II
1111►Y,1 )j111pVIIIW'111{1 1 iqu1 'NE LFAUEe 1N NVINONMENTAL iEBnNe
West SaCraIllEelO,CA 9$605 1111,WIIIV III1IVIIl`IIHI I I
phone [phone] fax [phone] Regulatory Program: ❑DW QNPDES ❑RCM ❑ota 11 'estAmeric ILaboratorles,Ina ?
Client Contact Project Manager. Nlek McCmcllen Sib Came Dr.La 320-3948 Chain of-Cars oNy X; No, 0 2013
1.
Evergreen Packaging ToWax:(US)646.2674 tab Conine 1 of Coca: _
175 Main Street Analysis Turnaround Time iampler.Univ roily of Tennessee
Canton.NC 2B716 ❑ CALENDAR DAYS El WOPMG DAYS -or Lab Use C nly:
828 646-67DO Phone TATdddremntnem Below Malk-m Client
[phone] FAX ❑ 2weft AbSampling.
Project Name,Fish Tissue Semplkig ❑ 1 weds
Site.Waterville Lake ❑ 2 days Job/SDO No.
POR ❑ Iday E S 2
oarnple
Semple Sample (T yp,, •or g - '
Sample Identification Dam Time Ga A) Maw Coat S SamPle a6e Notes:
S e C — 9 nva Yi (:
1( 1f a is
ayfw
CD
i
o
we. z Ate. S;k 0
n « rffmgy 5-&a 13qs 4 ilylow
6-I 1310 y
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l
�.
Possible Hazard Identification: - Sample Ohposal(A fee rosy be msnsed N1 Impinalenee longer than I month
Are any samples from a listed EPA Hazardous Waste? Please List any EPA Waste Codes for the sample In the
Comments Section tithe lab is to dispose of the sample I,
❑+ Nm Hamrd ❑Flammable LJ So ontmt - person a Unbmrm ❑Re um to dart ❑Dspmal W Lab Ar t, to1 Hands
Special InstructlonslOC Requirements&Commerce:
i
i I '
Custody Seals IntiV, 0 Ye No Custody See No.. ® er amp. en/n
lu Relinq she _ V r O /•j Recehr ` n O m ..
f.P CD Re0 q h Company. Cam wry, '7 `I
elinqu tl Co sterrime: Revs In Lebomto by Com
04 903
IF omi klajc CLWI-0112,Rev.L2.dated 04I02120113
e._.�r
X ® _c7 LUG LJ !�J L J L s I I LJ L'_J

0
Login Sample Receipt Checklist
FJ
Client:Evergreen Packageing FKABlue Ridge Paper Job Number:320-3948-1
Login Number:3948 List Source:TestAmerica Sacramento
List Number:1
Creator.Hytrek,Cheryl
Question Answer Comment
Radioactivity wasn't checked or is</=background as measured by a survey True
meter. 7
The coolers custody seal,if present,is intact. True
Sample custody seals,if present,are intact. NIA F3
The cooler or samples do not appear to have been compromised or True ❑
tampered with.
Samples were received on ice. True ri
Cooler Temperature is acceptable. True U I
Cooler Temperature is recorded. True
COG is present. True
COG is filled out In ink and legible. True i
COG is filled out with all pertinent information. True
Is the Feld Samplers name present on COC? N/A G1
lt
There are no discrepancies between the containers received and the COG. True U7
Samples are received within Holding Time. True
Sample containers have legible labels. True
f Containers are not broken or leaking. True
Sample collection date/times are provided. True
Appropriate sample containers are used. True
Sample bottles are completely filled. True
Sample Preservation Verified. NIA
There is sufficient vol.for all requested analyses,incl.any requested True
MS/MSDs
Containers requiring zero headspace have no headspace or bubble is True
<6mm(1/4').
Multiphasic samples are not present. True
Samples do not require splitting or compositing. True
Residual Chlorine Checked. N/A
TestAmerica Sacramento
Page 22 of 22 1/30/2014

APPENDIX C
BLUE RIDGE PAPER CANTON MILL FISH FILLET TISSUE
ANALYSIS RESULTS, 1990-2004
33

APPENDIX C
Table 64. Blue Ridge Paper Canton Mill Fish Fillet Tissue Results.
1990-2004
From: EA Engineering, Science, and Technology, Inc.
December 2004
36

A
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F

Table 6-4 (cont.).
1994 R.ecultsv* 199S Itesultsm
Number of Length Number of Length
Station Species Fish Range(mm) 2,3,7,8-TCDD(-) Station Species Fish Range(mm) 2,3,7,8-TCDDt t
1 Rockbass 6 156-185 ND(0,083) 1 Rock bass 10 162-205 ND(0.10)
RM 64.5 Redbreast sunfish 10 155-197 ND(0.10) RM 64.5 Rock bass 10 150-220 ND(0.26)
Black redhorse 3 367.435 ND(D.096) Black redhorse 7 375.464 ND(0.21)
2 Redbreast sunfish 10 176-206 ND(0.(Y73) 2 Redbreast sunfish 10 152-194 ND(0.20)
RM 59.0 Redbreast sunfish 10 160-210 ND(0.092) RM 59.0 Redbreast sunfish 10 161-188 ND(0.16)
Common carp 10 490.59D 0.99 Comon carp 10 435-664 1.7
3 Redbreast sunfish 10 148-196 ND(0.15) 3 Redbreast sunfish 10 170-206 ND(0.18)
RM 52.3 Redbreast sunfish 10 158 210 ND(0.074) RM 52.3 Redbreast sunfish 10 154 202 ND(0.20)
Common carp 10 456-565 0.74 Common carp 10 391-571 1.2
4A Black crappie 30 203-231 ND(0,085) 4A Largemouth bass 5 281-439 2.0
RM 41.5 Bluegill 10 185-205 ND(0.084) RM 41.5 Bloeg0l 10 167.199 ND(0.26)
Common carp 10 465.591 3.4 Common carp 10 520-615 5.8
4B Black crapple 10' 20D-215 ND(0.084) 4B Largemouth bass 9 248-391 0.68
RM 39.0 Black Grapple 10 195.220 ND(0.062) RM 39.0 Bluegill 8 158-216 ND(0.34)
Common carp 10 520.635 6.6 Common carp 4 532.626 11.0
5 Redbreast sunfish 6 129.239 ND(0.075) 5 Smallmouth bass 9 280.423 ND(0.11)
RM 19.0 Smallmouth bass 9 234442 ND(0.11) RM 19.0 Redbreast sunfish 7 163-192 ND(0.15)
Smallmouth buffalo 9 440-520 ND 0.06 Black redhorse, 7 44G481 ND f0.45)
Total Fish Filleted 163 156

Table 6-4 (cont.).
1996 Rusultf 1997 Results%)
Number of Length Number of Length '
Station Species Fish Range(mm) 2,3,7,8-TCDDo) Station Species Fish Range(man) 2,3,7,8-TCDD(-)
I Redbreast sunfish 5 154.185 ND(0.13) 1 Redbreast sunfish 5 144-161 ND(0.11)
RM 64.5 Rock bass 5 160-208 ND(0.085) RM 64.5 Rock bass 5 162-194 ND(0.23)
Black redhorse 5 401.440 ND(0,089) Black redhorse 4 291-424 ND(0.22)
2 Redbreast sunfish 5 179-197 ND(0.10) 2 Redbreast sunfish 5 183-200 ND(0.26)
RM 59.0 Redbreast sunfish 5 183-191 ND(0.12) RM 59.0 Redbreast sunfish 5 160-I81 DID(0.12)
Common carp 5 543-580 1.5 Common carp 5 506-615 1.4
3 Redbreast sunfish 5 184-19D ND(0.13) 3 Redbreast sunfish 5 187.202 ND(0.18) '
RM 52.3 Redbreast sunfish5 165-185 ND(0.13) RM 52.3 Redbreast sunfish 5 164-195 ND A lg) '
Common carp 5 516-630 0.87 Common carp 5 450-505 ND(0.33)
4A Black cmppie 5 216.233 ND(0.15) 4A Black crapppie 5 215 231 ND(0.27)
RM 41.5 Black crappie 5 215-229 ND(0.18) RM 41.5 Black crappie 5 220.230 ND(0.10)
Common carp 5 562.632 42 Common carp 5 220-230 20.
Channel catfish 5 419-482 2.0
413 Black cmpple 5 223.258 ND(0.11) 4B Black crappie 5 226.241 ND(0.17)
RM 39.0 Largemouth bass 5 278.310 ND(0.13) RM 39.0 Largemouth bass 5 270-360 ND(0.21)
Common carp 5 470.623 4.0 Common carp 5 605.690 11.0
Flathead catfish 5 430-540 0.62
5 Rock bass 4 169.186 ND(0.077) 5 Rock bass 5 143-214 ND A 15) .
RM 19.0 Small-oath bass 5 315454 ND(0.12) RM 19.0 -Smallmouth bass 5 278.367 ND(0.27)
Smallmouth buffalo 5 451-555 ND 0.12 Smallmouth buffalo 5 406.525 ND 0 22
Total Fish Filleted 89 Total Fish Filleted 99
i

Table 6-4 (cont.).
1998 Resultsra 1999 Results(b)
Number of Length Number of Length
Station Species Fish Range(min) 2,3,7,8-TCDDt't Station Species Fish Range(min) 2,3,7,8-TCDDt t
1 Redbreast sunfish 5 145-176 ND(0.19) 1 Redbreast sunfish 5 141-177 ND(0.21)
RM 64.5 Rock bass 5 158-179 ND(0.29) RM 64.5 Rock bass 5 164.190 ND(0.37)
Black redhorse 5 340-396 ND(0.18) Black redhorse 5 352-427 ND(0.33)
2 Redbreast sunfish 5 164-177 ND(0.20) 2 Redbreast sunfish 5. 167-190 ND(0'.37)
RM 59.0 Redbreast sunfish 5 166.193 ND(0.28) RM 59.0 Redbreast sunfish 5 158-178 ND(0.29)
Common carp 5 551661 1.3 Common carp 5 544.615 ND(0.27)
3 Redbreast sunfish 5 169-193 ND(034) 3 Redbreast sunfish 5 169-189 - ND(0.36)
RM 52.3 Redbreast sunfish 5 167-200 ND(0,22) RM 52.3 Redbreast sunfish 5 162-176 ND(0.37)
Common carp 5 449-550 ND(0.38) Common carp 5 500-591 0.57
4A Black crappie 5 220.240 ND(0.49) 4A Black crappie 5 220-268 ND(0.18)
RM 41.5 Largemouth bass 5 227-330 ND(0.15) RM 41.5 Black crappie 5 219-244 ND(0.08)
Common carp 5 585621 1.6 Common carp 5 574-645 0.58
Channel catfish 5 416458 ND(0.28) Channel catfish 5 425.482 0.83
4B Black crappie 5 233-252 ND(0.15) 4B Black erspple 5 233-244 ND(0.27)
RM 39.0 Largemouth bass 5 259.330 ND(0.17) RM 39.0' Largemouth bass 5 276-305 ND(0.32)
Common carp 5 563686 9.1 Common carp 5 621680 4.7
Flathead catfish 5 414.523 ND(0.20) Flathead catfish 5 372-513 ND(0.46)
5 Rock bass 4 155-M ND(0.11) 5 Rock bass 5 170.203 _ ND(029)
RM 19.0 Sroallmoudn bass 5 295-365 ND(0.21) RM 19.0 Smallsamah bass 5 297-430 ND(0.19)
Smalbnouth buffalo 5 464.537 ND 0.31 Smallmouthbuffalo 5 476-565 ND .31
Total Fish Filleted 99 Total Fish Filleted 100
I

Table 6-4 (cunt.).
2000 Results
2001 Resultst'o
Number of Length Number of Len Station Species Fish Range(tom) 2,3,7,8-TCDDt'> 901
Station Species Fish Range(mm) 2,3,7,8-TCDD(O
1 Redbreast sunfish 5 137-148 ND(0.48) I Black redhorse 5 312.4D7 ND(0.25)
RM 64.5 Rock bass 5 162-186 ND(0.45) RM 64.5
Black redhorse 5 357-396 ND(0.38)
2 Redbreast sunfish 5 169-176 ND(0.31) 2 Common carp 5 456-555 ND(0,27)
RM 59.0 Redbreast sunfish 5 164-181 ND(0.43) RM 59.0
Common carp 5 505-582 ND(0.42)
3 Redbreast sunfish 5 169-181 ND(0.43) 3 Common carp 5 5D4.615 ND(0.35)
RM 52.3 Redbreast sunfish 5 186-199 ND(0.32) RM 52.3
Common carp . 5 514-569 ND(0.53)
4A Black crappie 5 212-241 ND(0.29) 4A Channel catfish 5 476-612 1.2
RM 41.5 Black crappie 5 220-241 ND(0.24) RM 415 Common carp 5 528-668 1.3
Common carp 4 559-604 1.1
Charnel catfish 5 435-487 ND(0.70)
4B Black crappie 5 213.231 ND(0.41) 0 Flathead catfish 5 405.463 ND(0.29)
RM 39.0 Black crappie 5 220-230 ND(0.37) RM 39.0 Common carp 5 654-723 5.6
Common carp 4 593-712 4.4
Flathead catfish 5 407-45D ND(0,42)
5 Rock bass 5 171-198 ND(0.45) 5 Black redhorse 5 437-497 ND(0.26)
RM 19.0 Smaldmouth bass 5 209.238 ND(0.31) RM 19.0
Black reborse 5 427-476 ND 0.3
Total Fish Filleted 98 Toml Fish Filleted 40

Table 64 (cont.).
2002 Results(O 2003 Resultsro)
Number of Length Number of Station Species Fish Range(cam) 2,3,7,8-TCDDm Station Species Fish Length Range(mm) 2,3,7,8-TCDDat
1 Black redhorse 5 372-431 ND(0.14) 1 Black redhorse 5 343-420 RM 64.5 RM 64.5 ND(0.20)
2 Common carp 5 517.548 ND(0.28)
RM 59.0 RM 59.0 Common cup 5 512-584 ND(0.18)
3 Common carp 5 575-632 ND(0,22) 3 Common cupRM 52.3 RM 52.3 P 5 545-605 ND(0.31) '
4A Common carp 5 523-648 2.2 4A Common tarp 5 655-017 3.4
RM 41.5 Channel catfish 5 425A75 ND(0.31) RM 41.5 Flathead catfish 5 521.575 ND(DL=0.35)
4B Common carp 5 647-670 6.6 4B Common carp 5 602-745 12.0
RM 39.0 Flathead catfish 5 41M5 ND(0.22) RM 39.0 Flathead catfish 5 511-533 ND(DL-0.20)
RM
5 19.0 Black redhorse 5 430-089 ND(0.14) 5 Black redhorse 5 445-524 ND(DL-0.19)
RM 19.0
Total Fish Filleted 40 Total Fish Filleted 40

i
Table 6-4 (cont.).
2004 Resultsw
Number of Length
Station Species Fish Range(mm) 2,3,7,8-TCDDtn
RM
1 64.5 Black redhorse 5 352440 ND(0.11)
2 Common carp 5 545-668 ND(0.19)
RM 59.0 Channel catfish 5 369-453 ND(0.17)
3 Common carp 5 587-M ND(0.27)
RM 52.3
4A Common carp 5 598-655 1.7
RM 41.5 Flathead catfish 5 508-565 ND(0.30) .
4B Common carp 5 570.66D 1.6
RM 39.0 Channel catfish 5 485-542 ND(0,31)
RM
5 I9.0 Black redhorse 5 _ 420-480 ND(0.13)
'
Total Fish Filleted 40 .
(a) Survey conducted by EA Engineering,Science,and Technology. Analyses conducted by ENSECO Laboratories 1990-1994,.Quanterra Laboratories 1995-1999,Severs Trem Labommries in 2Q00.
2003.
(b) Survey conducted in August.
(c) Survey conducted in August and September. .
(d) Survey conducted is September.
(a) Units=ppt(parts per trillion)or pgfg(picogman pa gram)
ND=Non-detectable at the detection limit In parentheses.
i

APPENDIX D
2006 REVISIONS TO DIOXIN MONITORING PLAN
i
34

V A Michael F.Easley,Governor
\O�� 9QG William G.Ross Jr.,Secretary
North Carolina Department of Environment and Natural Resources
CO r Alan W.Klimek,P.E.Director
Division of Water Quality
Y LS(� Coleen H.Sullins,Deputy Director
nn C E 0 . . . ality
June 6,2006 JUN 12
Mr. Paul S.Dickens
Manager,Environmental Affairs BLUE USE PAPER PRODUCTS INC
Blue Ridge Paper Products BHS DEPARTMENt
P.O. Box 4000
Canton,North Carolina 28716
Subject: Revisions to Dioxin Monitoring Plan
Blue Ridge Paper Products
NPDES No.NCO000272
Dear Mr.Dickens:
The Water Quality Section has reviewed your request for modifications to the subject study plan
originally approved by the Division of Water Quality in February 1990. The 2001 dioxin fish
tissue monitoring plan is approved with the following changes:
• Elimination of main stem river sampling stations-beginning in 2006 fish tissue
samples will be collected only from Waterville Lake.
• Collect a least one whole body bottom feeder sample from Waterville Lake (stations
4A or 4B)to monitor ecological impacts of dioxin contamination in bottom species.
• Continue with the collection of bottom feeder fillet samples in Waterville Lake as
approved in the 1990 study plan.
After reviewing your request to eliminate whole body dioxin analyses from Waterville Lake,our
staff recommends that this analysis be continued. Although the Waterville Lake data submitted
in your 2005 report shows low levels of dioxin, data submitted by Progress Energy in 2005
showed a mean dioxin TEQ value of 6.7 pg/g for common carp. The value was above the 4.0
pg/g TEQ value used by NCDHHS to post fish advisories for dioxin. As this time the dioxin
advisory for Waterville Lake remains in effect,and in our view,warrants continued monitoring.
If you have any further questions or comments please feel free to contact Mark Hale at 919/733-
6946.
'ncerely,��
immie verton
ironmental Sciences Section
cc: Roger Edwards-ARO
Dr.Luanne Williams—NCDHHS
Susan Wilson -DWQ Permits
IJohn Crutchfield—Progress Energy MAT,
ENR
N.C.Division of Water Quality 1617 Mail Service Center Raleigh,North Carolina 27699.1617 [phone] Customer Service
1-877-623-6748

BLUE RIDGE COPY
PAPER PRODUCTS INC.
Certified Mail
Return Receipt Requested
7099 3220 0007 0371 6235
30 March 2006
Mr. Mark Hale
Environmental Biologist
Environmental Services Section
Division of Water Quality
North Carolina Department of Environment
and Natural Resources
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Subject: Study Plan Revision
Dioxin Monitoring in Fish Tissue—Waterville Lake
March 2006
NPDES No.NC0000272
/ Blue Ridge Paper Products Inc.
Canton Mill
Dear Mr. Hale—
The subject study plan revision is enclosed for Division of Water Quality(DWQ)review and
approval.
Background
The Blue Ridge Paper Products Inc. mill in Canton,North Carolina has completed biological
monitoring for dioxin in fish in the Pigeon River for the past 16 years. This annual biological
monitoring is a requirement of the Canton Mill's NPDES permit. The permit requires that we
follow a study plan approved by the DWQ for this work. Dioxin in fish sample collection and
reporting for 2002 through 2005 was conducted under the December 2001 Study Plan for
Pigeon River Dioxin Monitoring in Fish Tissue prepared by EA Engineering, Science and
Technology. EA Engineering completed the dioxin in fish sampling and reporting for years
2002 thru 2004. In 2005,Blue Ridge Paper contracted with the University of Tennessee,
Knoxville(UTK)Department of Forestry,Wildlife and Fisheries for the dioxin in fish tissue
work. UTK followed the 2001 Study Plan for the 2005 dioxin in fish sampling and report. The
2005 report was submitted to the DWQ on 20 Feb 2006.
J % 175 Main Street • PO Box 4000
Canton, North Carolina 28716. 828-646-2000
Raising Your Expectations

Mark Hale, NC DENR DWQ,ESS-
3/30/06, Page 2
Biological Monitoring Review
The first annual dioxin fish tissue sampling study of the Pigeon River was in 1990. At that time,
public health fish consumption advisories related to dioxin were in place for the Pigeon River
downstream of the Canton Mill for both North Carolina and Tennessee reaches of the river.
Dioxin fish tissue concentrations determined from annual sampling declined sharply following
completion of the Canton Mill modernization in 1993. Dioxin fish tissue sample concentrations
in bottom feeding species at main stem river sample locations became non-detect in 2000 and
have remained below analytical detection levels since that time. Dioxin fish tissue sample
concentrations in sport fish species from all sampling locations in the Pigeon River became non-
detect in the mid-1990s.
Based on annual fish tissue sampling, the State of North Carolina lifted the dioxin in fish
advisory for the Pigeon River upstream of Waterville Lake in August 2001. The State of
Tennessee lifted the dioxin in fish advisory for the Tennessee portion of the Pigeon River in
January 2003. The only fish advisory remaining is for carp in Waterville Lake: Dioxin levels in
carp collected from Waterville Lake since 2000 continue to be at or near analytical detection
levels and are below the North Carolina Department of Health and Human Services (NC DHHS)
dioxin toxicity equivalent(TEQ)fish advisory action level of 4 parts per trillion(ppt).
The NC DHHS has indicated that two consecutive years of TCDD TEQ levels in carp of 3 ppt or
f' less would provide basis for lifting the public health advisory for consumption of carp from
Waterville Lake(e-mail correspondence from Dr.Luanne Williams,DHHS to Blue Ridge Paper
dated 9 Mar 2006). The TCDD TEQs for carp fillet composite samples from Stations 4A&4B
in Waterville Lake for the last two years are as follows:
Year Station 4A Station 4B
2004 2.5 ppt 2.2 ppt
2005 2.1 ppt 3.6 ppt
Study Plan Revision
Blue Ridge Paper proposes the following changes to the 2001 biological monitoring Study Plan:
v elimination of main stem river sampling stations --fish tissue samples beginning in 2006
will be collected only from Waterville Lake
Blue Ridge Paper Products Inc
175 Main Street a PO Box 4000
Canton, North Carolina 28716 a 828-646-2000
Raising Your Expectations

Mark Hale, NC DENR DWQ,ESS-
3/30/06, Page 3
• elimination of whole body fish composite samples from the lake--only composite fillet
samples of target bottom feeding fish species will be collected for laboratory analysis of
dioxin.
The 2006 study plan revision is enclosed for your review and approval. A copy of the 2001
study plan is also enclosed for your reference.
Summary
The 2006 dioxin in fish sampling work is planned for the mid-summer period of August and
early September. We request DWQ approval of the 2006 study plan revision no later than the
end of May 2006.
Please contact us if you have questions.
Paul S.Dickens J. Glenn Rogers
Manager,Environmental Affairs Water Compliance Coordinator
828-646-6141 828-646-2874
[e-mail] [e-mail]
I '
U
Enclosures: Study Plan Revision,Dioxin Monitoring in Fish Tissue— Waterville Lake
March 2006
Prepared by University of Tennessee for Blue Ridge Paper Products
Study Plan for Pigeon River Dioxin Monitoring in Fish 7Yssue
December 2001
Prepared by EA Engineering for Blue Ridge Paper Products
cc(w/enclosures):
Bryn Tracy- NC DENR DWQ,ESS
Susan Wilson—NC DENR DWQ,Permits
Sergei Cherinkov—NC DENR DWQ,Permits
Roger Edwards—NC DENR DWQ,ARO
Keith Haynes -NC DENR DWQ, ARO
Dr. Louanne Williams—NC DHHS
Larry Wilson—UTK
Billy Clarke—Roberts &Stevens
John Crutchfield—Progress Energy
Blue Ridge Paper Products Inc
175 Main Street • PO Box 4000
Canton, North Carolina 28716. 828-646-2000
Raising Your Expectations

-' BLUE R.IDGE COPY
PRODUCTS RQDUCTs INC.
STUDY PLAN REVISION
DIOXIN MONITORING IN FISH TISSUE
Waterville Lake
Prepared for:
Blue Ridge Paper Products,Inc.
Canton Mill
Canton,North Carolina
NPDES No.NC 0000272
Prepared by:
J.Larry Wilson,Ph.D.
Departments of Forestry,Wildlife and Fisheries
University of Tennessee
Knoxville,Tennessee
March 2006

Study Plan—2006 Dioxin Monitoring in Fish Tissue,Waterville Iake
University of Tennessee for Blue Ridge Paper Products Inc.Canton Mill
Page I
INTRODUCTION
The Blue Ridge Paper Products Inc. Canton Mill conducts annual biological monitoring of
dioxin in fish in the Pigeon River trader conditions of the NPDES permit for the mill. This
monitoring for years 2002 thru 2005 was in accordance with the December 2001 Study Plan for
Pigeon River Dioxin Monitoring in Fish Tissue prepared by EA Engineering, Science and
Technology(EA 2001 a). This document details changes for dioxin in fish monitoring beginning
in 2006. The 2001 study plan is incorporated by reference. The specific changes to the 2001
study plan include:
• elimination of main stem river sampling stations --fish tissue samples in 2006 will be
collected only from Waterville Lake
• elimination of whole body fish composite samples from the lake-- only composite fillet
samples of target bottom feeding fish species will be collected for laboratory analysis of
dioxin.
The 2006 dioxin in fish tissue sampling from Waterville Lake will be conducted by personnel
from the University of Tennessee, Knoxville(UTK),Department of Forestry,Wildlife and
Fisheries.
SAMPLE LOCATIONS
The schedule for fish tissue collection will be consistent with prior years targeting dry weather
periods in late August and early September. UTK will collect and prepare fish tissue samples
from two locations in Waterville Lake on the Pigeon River(PRM 39.0-41.5). In previous
studies,the fish collection sites in Waterville Lake have been designated 4A(PRM 41.5)and 4B
(PRM 39.0). Site 4A is located approximately 21.8 RM downstream from the Canton Mill
outfall,near the Messer Branch-Pigeon River confluence. Site 4B is in the vicinity of the
Wilkins Creek-Pigeon River confluence, approximately 24.3 RM downstream from the Canton
Mill outfall. Fish sampling will be conducted in the vicinity of each location described above;
however, the distance or effort at each station will depend on how difficult it is to collect target
fish species at that location. In 2005, common carp in the lower portion of the Waterville Lake
were extremely difficult to collect.
FISH COLLECTION TECHNIQUES AND TARGET SPECIES
The goal of the 2006 fish collection effort is one composite bottom feeder fillet sample at each
Waterville Lake sampling station 4A and 4B. Each composite consists of 3 to 5 similarly sized
(shortest specimen within 75% of the length of the longest) adult individuals of the target
species. Common carp (Cyprinus carpio) and channel catfish(Ictalums punctatus) are the
targeted bottom feeder species at sites 4A and 4B. Every reasonable effort will be made to
collect the desired size, species, and number of fish. However,the outcome of sampling effort

Study Plan—2006 Dioxin Monitoring in Fish Tissue,Waterville Lake
University of Tennessee for Blue Ridge Paper Products Inc.Canton Mill
Page 2
I
each year is dependent on physical river conditions and the natural diversity and abundance of
target fishes at each location.
The field investigators will be equipped with an array of collecting gear, which will enable
sampling of all habitats regardless of river conditions. The US EPA recommends active methods
of fish collection in the Sampling Guidance Manual (Versar 1984),such as electrofishing,
trawling, angling, or seining. These are preferred over passive methods (e.g., gill nets, trap nets,
trot lines)because samples are collected from more defineable areas (Versar 1984). Electro-
fishing may be used at both locations (4A and 4B); gill nets may also be used in areas where
water depth will limit the electro-fishing gear. A boat electrofishing unit(pulsed direct current,
Smith Root Type VIA electro-fisher powered by a 240-volt, 5000-Watt generator) will be
provided. Electro-fishing techniques will follow those described in the National Dioxin Study
(Versar 1984). All gill nets will be pulled and examined on a regular basis to reduce specimen
stress or mortality, and specimens submitted for analysis will be selected for good health and
condition.
Fish collection techniques and level of effort(time) expended at both locations (4A and 4B) will
be recorded. Total study effort for the 4A/4B collections in 2005 took 460 gill-net hours and
approximately six hours of electro-fishing over a four-day period to obtain the necessary
specimens. Channel catfish were easily caught,but common carp were extremely difficult to
collect,particularly in the lower portion of the lake(4B).
In summary,fish tissue samples will be collected as follows:
• bottom-feeder composite—one composite bottom feeder fillet sample at each location
4A and 4B
• catfish composite—one composite catfish fillet sample at each location 4A and 4B
SAMPLE PREPARATION
All fish tissue samples will be prepared in accordance with U.S.EPA Region IV
recommendations (Cummingham 1990 as described in EA 2001a). To prevent cross-
contamination between sampling stations, all sampling equipment likely to come into contact
with the fish will be rinsed extensively with site water between stations.
Specimens collected at each location will be sorted by size and species, and target species
identified. UTK will obtain a 3 to 5-fish composite sample at each location(4A and 4B) which
meet the species/size objectives discussed earlier. All specimens retained will be immediately
placed on ice for later processing. All fish submitted for tissue analysis will be measured to the
nearest millimeter(TL) and weighed to the nearest gram(wet weight).
Following collection of channel catfish and common carp, each specimen will be prepared for
shipment and analysis. Samples will consist of epaxial muscle tissue and skin from one side of
the fish. Fillet samples will be prepared by removing scales (or removing the skin from

Study Plan—2006 Dioxin Monitoring in Fish Tissue,Waterville Lake
University of Tennessee for Blue Ridge Paper Products Inc.Canton Mill
Page 3
catfishes) and Shen making an incision behind the opercula(on both sides of the fish)from the
base of the spine (behind the skull) to just below the pectoral fin. Care will be taken to cut
through the epaxial muscle without puncturing the rib cage or gut lining. A second incision will
be made along the length of the spine to the caudal fin on both sides of the fish. The muscle will
be cut away from the rib cage to obtain a fillet. Fillet knives will be solvent rinsed(hexane and
acetone)between fish from different locations. Each composite sample will be wrapped in
aluminum foil (dull side toward sample),labeled, and placed on dry ice. One side fillets will be
sent to Sevem Trent Laboratory for analysis; the opposite side fillets will be retained by Blue
Ridge Paper as back-up fillets.
All individual specimens (fillets) composing a single composite sample will placed together in a
water-tight plastic bag labeled with the station name, sample number, and the number of samples
in that composite. All labels will contain following information: sample identification number,
sample location and station identification, sampling team initials,date of collection, species
name, and sample type(i.e.,fillet). A chain-of-custody form will be filled out for each cooler of
samples submitted for analysis, and each form will include composite specific information and
instructions.
All samples will be frozen solid prior to shipment to the analytical laboratory. The frozen
samples will be packed on dry ice and shipped via overnight delivery to Severn Trent Laboratory
(STL)—Sacramento for analysis.The back-up fillets will be retained in a freezer at the Canton
Mill until laboratory analytical results for the composite fillet samples are received and verified;
the back-up fillet samples will then be destroyed.
SAMPLE ANALYSIS
The composite fillet fish tissue samples received by STL will be analyzed for dioxin(2,3,7,8-
TCDD), furan(2,3,7,8-TCDF) and related isomers using EPA Method 8290(US EPA 1994).
'Percent lipids will also be determined for each composite sample. STL will provide laboratory
analytical reports including QA/QC summaries and completed chain-of-custody forms
documenting receipt by the lab.
REPORTING
Results of 2006 dioxin in fish tissue sampling from Waterville Lake will be submitted to the.NC
DENR within 180 days of the completion of field work as required by the Blue Ridge Paper
NPDES permit. The fish tissue sampling and analytical report will follow the general format of
prior year reports. Dioxin toxicity equivalent(TEQ) levels will be calculated for all 17
chlorodibenzo dioxin and furan(CDD/F) isomers included in EPA Method 8290. The TEQ of
each detectable isomer will be calculated based on the toxicity equivalent factor(TEF)for the
isomer provided by the World Health Organization(WHO 1997). The measured concentration
of each CDD/F isomer will be multiplied by its appropriate TEF to obtain a concentration of the
isomer equivalent to the toxicity of 2,3,7,8-TCDD,the most toxic of the CDD/F isomers. Non-
detectable concentrations will be treated as zero values for TEQ calculation. As requested by the

Study Plan—2006 Dioxin Monitoring in Fish Tissue,Waterville Lake
University of Tennessee for Blue Ridge Paper Products Inc.Canton Mill
Page 4
NC DHHS (Williams 2006),the TEQ results for the last two years will be presented in a
columnar, side-by-side format to allow easy comparison against health advisory action levels.
References
EA Engineering,Science,and Technology Inc. 1990. Study Plan for the Monitoring of Dioxin in Fish
Tissue. EA Report No. 11370.01,prepared for Champion International Corporation,Canton,
North Carolina.
EA Engineering,Science,and Technology Inc. 1991. Results of the 1990 Dioxin Monitoring in Fish Tissue.
EA Report No. 11370.02,prepared for Champion International Corporation,Canton,North
Carolina.
EA Engineering,Science,and Technology Inc. 1992. Results of the 1991 Dioxin Monitoring in Fish Tissue.
EA Report No. 11370.03,prepared for Champion International Corporation, Canton,North
Carolina.
EA Engineering,Science,and Technology Inc. 1993a. Results of the 1992 Dioxin Monitoring in Fish Tissue.
EA Report No. 11370.05,prepared for Champion International Corporation,Canton,North
Carolina. April.
EA Engineering,Science,and Technology Inc. 1993b. Results of the 1993 Dioxin Monitoring in Fish
Tissue. EA Report No. 11370.06,prepared for Champion International Corporation,Canton,
North Carolina. December.
I
EA Engineering,Science,and Technology Inc. 1994. Results of the 1994 Dioxin Monitoring in Fish Tissue.
EA Report No. 11370.07,prepared for Champion International Corporation,Canton,North
Carolina. December.
EA Engineering,Science,and Technology Inc. 1995. Results of the 1995 Dioxin Monitoring in Fish Tissue.
EA Report No. 13043.01,prepared for Champion International Corporation,Canton,North
Carolina. December.
EA Engineering,Science,and Technology Inc. 1996. Results of the 1996 Dioxin Monitoring in Fish Tissue.
EA Report No. 13176.01,prepared for Champion International Corporation,Canton,North
Carolina. December.
EA Engineering,Science,and Technology Inc. 1997. Results of the 1997 Dioxin Monitoring in Fish Tissue.
EA Report No. 13353.01,prepared for Champion International Corporation,Canton,North
Carolina. November.
EA Engineering,Science,and Technology Inc. 1998. Results of the 1998 Dioxin Monitoring in Fish Tissue.
EA Report No. 13478.01,prepared for Champion International Corporation,Canton,North
Carolina. December.
EA Engineering,Science,and Technology Inc. 2000. Results of the 1999 Dioxin Monitoring in Fish Tissue.
EA Report No. 13478.01,prepared for Champion International Corporation,Canton,North
Carolina. January.
EA Engineering,Science,and Technology Inc. 2001a. Study Plan for Pigeon River Dioxin Monitoring in
1, / Fish tissue. Prepared for Blue Ridge Paper Products,Canton,North Carolina. November.

Study Plan—2006 Dioxin Monitoring in Fish Tissue,Waterville Lake
University of Tennessee for Blue Ridge Paper Products Inc.Canton Mill
Page 5
EA Engineering,Science,and Technology Inc. 2001b. Results of the 2000 Dioxin Monitoring in Fish
Tissue. EA Report No. 13745.01,prepared for Blue Ridge Paper Products,Canton,North Carolina.
December.
EA Engineering,Science,and Technology Inc. 2001c. Results of the 2001 Dioxin Monitoring in Fish Tissue.
EA Report No. 13900.01,prepared for Blue Ridge Paper Products,Canton,North Carolina.
December.
EA Engineering,Science,and Technology Inc. 2003. Results of the 2002 Dioxin Monitoring in Fish Tissue.
EA Report No. 13900.02,prepared for Blue Ridge Paper Products,Canton,North Carolina.
December.
EA Engineering,Science,and Technology Inc. 2004. Results of the 2003 Dioxin Monitoring in Fish Tissue.
EA Report No. 13900.03,prepared for Blue Ridge Paper Products,Canton,North Carolina.
January.
Henry,A.G.and J.L.Wilson. 2006. Results of 2005 Dioxin Monitoring in Fish Tissue. Center for
Environmental Biotechnology and Department of Forestry,Wildlife and Fisheries, University of
Tennessee,Knoxville,Tennessee.
U.S.Environmental Protection Agency(US EPA). 1994. Analytical Procedures and Quality Assurance for
Multimedia Analysis of Polychlorinated Dibenzo-para-dioxins and Polychlorinated Dibenzofurans by
High Resolution Gas Chromotography/High Resolution Mass Spectrometry(Method 8290).
I - Versar,Inc. 1984. Sampling Guidance Manual for the National Dioxin Study. U.S.Environmental
Protection Agency. EPA Contract 68-01-6160. Work Order Number 8.7. Office of Water
Regulations and Standards,Monitoring and Data Support Division,Washington,D.C.Final Draft
July.
Williams, L. 2006. E-mail correspondence from North Carolina Department of Health and Human Services
to Blue Ridge Paper Products concerning review of 2005 Dioxin Monitoring in Fish Report, March
9.
World Health Organization(WHO). 1997. Exposure and Human Health Reassessment of 2,3,7,8-
Tetrachlorodibenzo-p-dioxin(TCDD)and Related Compounds: Part lI Health Assessment of
2,3,7,8-Tetrachlorodibenzo-P-dioxin(TCDD and Related Compounds.

COPY
EA Project 13900.01
STUDY PLAN FOR PIGEON RIVER
DIOXIN MONITORING IN FISH TISSUE
Prepared for.•
Blue Ridge Paper Products
Canton, NC
Prepared by.
EA Engineering, Science, and Technology
444 Lake Cook Road, Suite 18
Deerfield, IL 60015
December2001

TABLE OF CONTENTS
Page
I..........Introduction .................................................................................... 1-1
2. Station Locations .............................................................................. 2-1
3. Fish Collection Techniques and Target Species .......................................... 3-1
4. Sample Preparation ........................................................................... 4-1
5. Analysis of Fish Tissue Samples ............................................................ 5-1
6. Quality Assurance ............................................................................. 6-1
7. Project Schedule ............................................................................... 7-1
8. Monitoring Report ....................................................:........................ 8-1
9. References ...................................................................................... 9-1
i

1. INTRODUCTION
This study plan details the materials and methods that will be used to annually assess the levels
of dioxins and furans in fish tissues from the Pigeon River downstream of the discharge from
the Blue Ridge papermill in Canton,North Carolina. This study plan was developed in
response to requirements in the mill's current NPDES permit(Permit No.NC0000272).
Changes to original permit conditions as detailed in letters from North Carolina Division of
Water Quality(NCDWQ)and North Carolina Department of Health and Human Services
(NCDHHS)dated 22 August 2001 and 31 August 2001,respectively have been incorporated
into this study plan. Section 2 of this study plan provides information regarding the locations
of the sampling stations. Section 3 details the techniques that will be used to collect fish at
each station and the target species. Section 4 describes the procedures that will be used to
prepare the collected fishes for tissue analysis. Section 5 specifies the analytical technique that
will be used to determine the concentrations of the different dioxin and furan isomers in each
tissue sample. The Quality Assurance plan is presented in Section 6,and the project schedule,
monitoring report, and references are found in Sections 7, 8, and 9,respectively.
1

2. STATION LOCATIONS
Six stations have been established for the collection of fishes (Figure 2-1 and Table 2-1).
Station 1,the background site,is located at Pigeon River Mile(RM)64.5 adjacent to the
Canton Recreational Park, approximately 1.2 RM upstream from the Canton Mill outfall.
Except at very high flows,the Canton Mill dam blocks the movement of fishes thereby
preventing the interaction of control and monitoring station fishes. Station 2 is located at RM
59.0,upstream from Clyde,NC and approximately 4.3 RM downstream from the Canton Mill
outfall. Station 3 is located at RM 52.3 just upstream of the old Rt.209 bridge, approximately
11.0 RM downstream from the Canton Mill outfall. Stations 4A and 4B are located in
Waterville Lake at RM 41.5 and 39.0,respectively. Station 4A is located approximately 21.8
RM downstream from the Canton Mill outfall,near the Messer Branch-Pigeon River
confluence. Station 4B is in the vicinity of the Wilkins Creek-Pigeon River confluence,
approximately 24.3 RM downstream from the Canton Mill outfall. Stations 4A and 4B
correspond to the upper and lower ends,respectively,of Waterville Lake. Station 5 is at RM
19 near Bluffton,TN,44.3 miles downstream of the mill outfall. Fish sampling will be
conducted in the immediate vicinity of each location described above,however, the sample
reach may be extended somewhat, if necessary,to facilitate collection of the required number
of fish.
2

1.40
Station 5 ..
RM 19.0 5
Bluffton TN--
(19.35
Hertford,TN
TENNESSEE N
NORTH
... — CAROLINA
Bi0 Hydro Plant I a
(26.0) ` o'
r
e
`m
n �I gP
5
4� Walters Dam— B
Station 46 Station 4A
RM 39.0 RM 41.5
4
Waterville Lake
Fines Creek
Now Hepeo Bridge
FLOW ✓onathans
Creek Old Rt.209
3 Station 3 Station 2 Mill Outfall
RM 52.3 RM 50.0 RM 63.3
Waynesville STP— 2 Canton,NC
Ouffall D
RM SOA �Q��
Q?oGtee Clyde Station 1 1
RM 64.5
(Control)
Figure 2-1. Fish tissue sampling station locations on the Pigeon River.

i
TABIE 2-1 PIGEON RIVER SAMPLING STAT1ON5
Station River Station Location and
Numb er Ile Distance from Outfall Site DescrintioNHabital Type Fish Common*ro
1 64.5 Pigeon Riverupstream from Canton, Characterized by riffle,ion,and pool habitats. Maximum Relative abundance dominated by minnows and darters. River
NC,adjacent to Canton Recreational depth approximately 6 tL Substrate primarily cobble and chub,greenfin darter and rock bass am dominant. Nortbem hog
Park(1.2 RM upstream from Canton boulders interspersed with gravel and sand. sucker,black redhorse,mirror shiner,redbreast sunfish,central
Mill outfall). stoneroller,gri enside darter,and mottled scullim am common.
2 59.0 Pigeon River upstream ftom Clyde,NC Characterized by riffle,rim,and pool habitats with canopy Relative abundance dominated by redbreast sunfish,central
(4.3 RM downstmam from the Contort cover. Maximum depth approximately 5 ft. Substrate stommoller,and northern hog sucker.
Mill outfall). primarily cobble/gravebsand with someboulders and bedrock.
3 52.3 Pigeon River in the vicinity of the Rt. Characterized by rif ce ran,and pool habitats with some Redbreast suofisb,northern hog sucker,and common carp am
209 bridge(11.0 RM downsumm canopy cover. Maximum depth approximately 5 R. dominant. Central stoneroller is common.
from the Canton Mill outfall) Substrate primarily bedrock and bouldets with some i
cobble and sand/gravelffines deposited in pool areas. i
4A 41.5 UpperWmerville Like(21.8 RM Characterized by deep-water fertile habitat,bedrock, Relative abundance dominated by black crappie. Bluegill,
downstream from the Canton Mill and soft bottom sediments. Maximum depth sampled flathead catfish,channel catfish,largemouth bass,and common
outfall) approximately 14 ft. coup are common.
i
i
4B 39.0 Lower Waterville lake(243 RM Characterized by deep-water lentic habitat.bedrock, Relative abundonmofcatch dominated by black crappie.
downstream from the Canton MITI and softbottam sediments. Maximum depth sampled Bluegill,channel catfish,Flathead catfish,and carp am common.
outfall) approximately 40 ft. Very steep banks with little cover.
5 19.0 Pigeon Rivernear BlufRoa,TN,just Characterized by deep pools and runs with some shallow Central stoneroller and nurthem hog sucker are dominant. I
upstream of 140(443 RM downstream ilium Maximum depth approximately 6 to 8 FL Substrate Smallmouth bass,whitetail shiner,telescope shiner,greensick I
from the Canton Mill omfal0 primarily bedrock boulder,and cobble, darter,and banded sculpin are common, Except for
smalim pith bass and rock bass,centramhids am uncommoon.
fF:

3. FISH COLLECTION TECHNIQUES AND TARGET SPECIES
A listing of the Pigeon River fish collection techniques is provided below:
Station Number River Mile Sampling Technique
1 64.5 Electrofishing
2 59.0 Electrofishing
3 52.3 Electrofishing
4A 41.5 Gill nets
4B 39.0 Gill nets
5 19.0 Electrofishing
Except in Waterville Lake, the principal sampling gear will be electrofishing. Depending on
depth,either a pram or boat electrofisher will be used. Gilinetting is the most effective
technique for the collection of bottom-feeder species from the Waterville Lake monitoring
stations. Gill nets will be checked at least once a day,usually within 12 hours of being
deployed. Other appropriate sampling techniques may be used if necessary.
Field notes will be recorded at each sampling station including the type of sampling gear,level
of effort(time),flow and clarity conditions,and selected physiochemical data(e.g.,dissolved
oxygen,water temperature,and conductivity[eectrofishing locations only])using accepted
proceedures and equipment. All fishes submitted for tissue analysis will be measured(total
( length),weighed(wet weight),examined for anomalies,and recorded on a standard Fisheries
Data Sheet(Figure 3-1).
The goal of the fish collection effort is to collect one composite bottom feeder fillet sample at
each of the six sampling stations. Each composite will consist of 3 to 5 similarly sized(shortest
specimen within 75%of the length of the longest)adult individuals of the target species.
Common carp will be the target species at Stations 2,3,4A, and 4B,with northern hog sucker
and black redhorse as alternative species in the riverinc portion of the river below the mill(i.e.,
Stations 2 and 3). Common carp are absent at Stations 1 and 5. At these stations,black
redhorse will be the target bottom feeder,with northern hog sucker and smallmouth buffalo
being alternative species. Alternative representative bottom feeder species may be collected in
the rare event that these target species are not collected.
In Waterville Lake,one additional fillet composite sample using either channel catfish or
flathead catfish will be collected at Stations 4A and 4B. Catfish are raze in the river between
the mill and Waterville Lake. However,in the event that three or more catfish of a similar size
are encountered at either Stations 2 or 3, a catfish fillet composite will be prepared for either or
both of these stations.
In addition to the fillet composites, a whole body composite consisting of 3-5 similarly sized
common carp will be collected at either Station 4A or 4B in Waterville Lake.
5

C
Table 3-1 Fish Collection Techniques
Station Number Station Location Sampling Technique
1 Pigeon River Mile 64.5 Electrofishing
2 Pigeon River Mile 59.0 Electrofishing
3 Pigeon River Mile 52.3 Electrofishing
4A Pigeon River Mile 41.5 Gill nets
4B Pigeon River Mile 39.0 Gill nets
5 Pigeon River Mile 19.0 Electrofishing

In summary,fish will be collected as follows:
c Bottom feeder fillet composite—one sample at all six stations
o Catfish fillet composite—one sample at Stations 4A and at 4B
o Common carp whole body composite--one sample from either Station4A or 4B
o Catfish fillet composite—one sample at Stations 2 and at 3,if specimens are encountered
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4. SAMPLE PREPARATION
Fillet samples will consist of the epaxial muscle tissue from one side of the fish. The fillet
from the other side will be retained as a backup until laboratory results are obtained. Bottom
feeder whole-body samples will consist of the entire fish. Specimens collected will be rinsed in
site water,stunned with a sharp blow to the head and placed on wet ice until processing.Each
fish selected for analysis will be weighed and measured. Fillet samples will be prepared by
removing all scales (or removing skin from catfishes)and subsequently making an incision
behind the opercula(on both sides of the fish)from the base of the spine(behind the skull)to
just below the pectoral fin. Care will be taken to not puncture the gut lining. A second incision
will be made along the length of the spine to the caudal fin on both sides of the fish. The
epaxial muscle will then be gently cutaway from the rib cage to obtain a fillet. In this fashion,
all flesh and skin(except catfishes)will be obtained from head to tail. The fillets from
specimens comprising a particular composite will be combined,then the sample will be
wrapped in aluminum foil(dull side toward sample),placed in a water-tight plastic bag,labeled
(see Section 6), and placed on wet ice. Within 24 hours of processing,the samples will be
frozen.
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5. ANALYSIS OF FISH TISSUE SAMPLES
Samples will be analyzed for dioxin using EPA Method 8290(U.S.EPA 1994). Percent lipids
will also be determined for each sample. The laboratory will measure the concentration of 17
isomers of chlorodibenzo dioxins and furans (CDD/n. The toxic equivalent(TEQ)of each
detectable isomer will be calculated based on toxicity equivalent factors (TEF)provided by the
World Health Organization(WHO 1997). The measured concentration of each CDD/F isomer
will be multiplied by its appropriate TEF to obtain a concentration of that isomer equivalent to
the toxicity of 2,3,7,8-TCDD,the most toxic of the many CDD/F isomers. As per the directive
of NCDHHS,non-detectable concentrations will be treated as zero values for TEQ calculations.
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6. QUALITY ASSURANCE
The following procedures will be followed to prevent contamination of samples collected at
different stations or between composites collected at the same station. Dip nets,fish scalers,
and holding containers will be rinsed with site water prior to use at each station. All fish will
be rinsed in site water to remove any debris(e.g., sediment) prior to processing. During
processing,the measuring board will be wrapped with clear plastic wrap and this wrap will be
changed among stations. Fillet knives will be solvent rinsed(hexane and acetone)between fish
from different stations. Each composite sample will be wrapped in aluminum foil(dull side
toward sample),labeled, and placed on dry ice within 24 hours of processing.
All fillets or whole bodies composing a single composite sample will be foil-wrapped, then
placed in a water-tight plastic bag labeled with the station name,sample number,and the
species in that composite. All labels will contain the following information
c Sample identification number,
• Station identification,
o Sampling team initials,
• Date of sample collection,
• Species name,
• Sample type(i.e.,fillet or whole body)
q A unique composite number
Chain-of-Custody(COC)sheets will be completed following sample preparation and
compositing. The composite identification number and corresponding number and type of fish
comprising that composite sample will be recorded on the COC. Copies of all COCs will be
retained.
Following completion of sampling,samples will be shipped on dry ice to the analytical
laboratory via an overnight courier. Upon receipt,the laboratory will compare the contents
with that noted on the COC sheets and will note the condition of the samples.
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7. PROJECT SCHEDULE
To be consistent with past collections,all samples will be collected in August or September. A
final report will be provided to NCDEQ within 180 days of sampling.
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8. MONITORING REPORT
Each annual monitoring report will include the sampling methods and procedures employed, a
description of the sampling locations,descriptions of each fish collected(e.g.,species,length,
and weight),as well as the results of the chemical analysis(reported as individual TCDD/F
isomers and 2,3,7,8-TCDD Toxic Equivalent Values). Also included in appropriate appendices
will be field notes,copies of all chain-of-custody sheets,and any pertinent memorandum or
communication record forms.
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9. REFERENCES
U.S.Environmental Protection Agency(U.S.EPA) 1994. Analytical Procedures and Quality
Assurance for Multimedia Analysis of Polychlorinated Dibenzo-para-dioxins and
Polychlorinated Dibenzofurans by High-Resolution Gas Chromatography/High-
Resolution Mass Spectrometry(Method 8290).
World Health Organization(WHO). 1997. Exposure and Human Health Reassessment of
2,3,7,8-Tetrachlomdibenzo-p-dioxin(TCDD) and Related Compounds: Part 11 Health
Assessment of 2,3,7,8-Tetrachlorodibenzo-p-dioxin(TCDD)and Related Compounds.
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