NC0081736_Renewal (Application)_20201028

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Secretory -th, ,."'
rrecaS. DANIEL SMITH NORTH CAROLINA
Director Environmental Quality
October 28, 2020
Pender County
Attn: Chad McEwen, City Manager
PO Box 5
Burgaw, NC 28425-0005
Subject: Permit Renewal
Application No. NC0081736
Melinda B Knoerzer Adaptive Ecosystem WWTP
Pender County
Dear Applicant:
The Water QualityPermittingSection acknowledges the October 28, 2020 receipt of your permit renewal application and
9 P
supporting documentation. Your application will be assigned to a permit writer within the Section's NPDES WW permitting
branch. Per G.S. 150B-3 your current permit does not expire until permit decision on the application is made.
Continuation of the current permit is contingent on timely and sufficient application for renewal of the current permit. The
permit writer will contact you if additional information is required to complete your permit renewal. Please respond in a
timely manner to requests for additional information necessary to allow a complete review of the application and renewal
of the permit.
Information regarding the status of your renewal application can be found online using the Department of Environmental
Quality's Environmental Application Tracker at:
https://deq.nc.gov/permits-regulations/permit-guidance/environmental-application-tracker
If you have any additional questions about the permit, please contact the primary reviewer of the application using the
links available within the Application Tracker.
Sincerely,
aCtiAl_624.,
Wren Thedford 1
Administrative Assistant
Water Quality Permitting Section
cc: Central Files w/application
ec: WQPS Laserfiche File w/application
North Caro ra D_partrrertof Envronmerstai Qua ty I D:vsor,of Water Resocrzes
1). Q) W' n ngton Regora Off De 1127 Cardona!Drve Exterson I Wi?rnrngton,North Caro re 28405
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Pc
October 5, 2020
Division of Water Resources
Water Quality Permitting Section—NPDES
1617 Mail Service Center
Raleigh, NC 27699-1617
Subject: Pender County Utilities (PCU)—NPDES Permit NC0081736—Permit Renewal Application
To Whom It May Concern:
Enclosed please find a signed original and a copy of the NPDES permit renewal application package for
the US 421 Wastewater Treatment Facility located in Wilmington, North Carolina.The current NPDES
permit was issued effective March 9,2018, and will expire on July 31, 2021.
The enclosed NPDES permit application package consists of the following:
1. Completed EPA Form 2A RECEIVED
2. Tables A, B, C, and E
3. Topographic Map OCT 2 8 2020
4. Process Flow Diagram and Schematic NQ>'dR/ PDEs
5. Plant Process Narrative
6. 2020 Toxicity Testing Results Using Two Species
NPDES application Form 2A describes the sanitary sewer received from the Pender County Commerce
Park.The 421 Wastewater Facility discharges water to the Cape Fear River through outfall 001 after the
UV disinfection process. At this time the facility does not have a pretreatment program but has
submitted a pleminary to the PERCS unit with the Department of Environmental Quality.
Table C for the Effluent Parameters for Selected POTWS did not include data from 2018.This data can
not be found by the contracted laboratory or the Wastewater Treatment Facility.Therefore,the number
of samples data was calculated from 2019 and 2020 data.
If you have any questions or need additional information, please contact Amy Cox (Utilities Analyst) at
[phone], or by email at [e-mail].
Chad McEwen
Pender County Manager
605 East Fremont Street- PO Box 995
Burgaw, NC 28425
Phone: [phone] Fax: [phone]

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
Form U.S.Environmental Protection Agency
2A &EPA Application for NPDES Permit to Discharge Wastewater
NPDES NEW AND EXISTING PUBLICLY OWNED TREATMENT WORKS
SECTION 1.BASIC APPLICATION INFORMATION FOR ALL APPLICANTS(40 CFR 122.21(j)(1)and(9))
1.1 Facility name
Melinda K.Knoerzer Adaptive Ecosystem Wastewater Treatment Facility(US 421 WWTF)
Mailing address(street or P.O.box)
P.O.Box 995
City or town State ZIP code
0 Burgaw NC 28425
Contact name(first and last) Title Phone number Email address
Kenny Keel,P.E. Utilities Director [phone] [e-mail]
Location address(street,route number,or other specific identifier) D Same as mailing address
es 469 Quality Way
uL
City or town State ZIP code
Wilmington NC 28401
1.2 Is this application for a facility that has yet to commence discharge?
❑ Yes 4 See instructions on data submission ❑✓ No
requirements for new dischargers. F E i1` ,�D
1.3 Is applicant different from entity listed under Item 1.1 above?
❑ Yes ❑✓ No 4 SKIP to Item 1.4. ®CT 2 8 2020
Applicant name VCDEQ/DWR/NPDES1/
Applicant address(street or P.O. box)
0
City or town State ZIP code
0
Contact name(first and last) Title Phone number Email address
1.4 Is the applicant the facility's owner,operator,or both?(Check only one response.)
✓❑ Owner ❑ Operator ❑ Both
1.5 To which entity should the NPDES permitting authority send correspondence?(Check only one response.)
El Facility ❑� Applicant ❑ Facility and applicant
(they are one and the same)
1.6 Indicate below any existing environmental permits.(Check all that apply and print or type the corresponding permit
number for each.)
Existing Environmental Permits
❑ NPDES(discharges to surface ❑ RCRA(hazardous waste) ❑ UIC(underground injection
water) control)
NC0081736
❑ PSD(air emissions) ❑ Nonattainment program(CAA) 0 NESHAPs(CM)
to ❑ Ocean dumping(MPRSA) ElDredge or fill(CWA Section ❑ Other(specify)
404)
EPA Form 3510-2A(Revised 3-19) Page 1

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
1.7 Provide the collection system information requested below for the treatment works.
Municipality Population Collection System Type Ownership Status
Served Served (indicate percentage)
480 10o %separate sanitary sewer 0 Own 0 Maintain
V Pender %combined storm and sanitary sewer ❑ Own 0 Maintain
m Commerce Park ❑ Unknown ❑ Own ❑ Maintain
c %separate sanitary sewer ❑ Own 0 Maintain
to
%combined storm and sanitary sewer ❑ Own ❑ Maintain
= 0 Unknown 0 Own ❑ Maintain
a.
a %separate sanitary sewer 0 Own ❑ Maintain
c %combined storm and sanitary sewer ❑ Own 0 Maintain
0 ❑ Unknown ❑ Own 0 Maintain
E %separate sanitary sewer ❑ Own ❑ Maintain
N %combined storm and sanitary sewer 0 Own 0 Maintain
c ❑ Unknown 0 Own 0 Maintain
Total 480
0 Population
c., Served
Separate Sanitary Sewer System Combined Storm and
Sanitary Sewer
Total percentage of each type of o 0
sewer line(in miles) 100 /° 0 /o
'' 1.8 Is the treatment works located in Indian Country?
c
o ❑ Yes ✓❑ No
0
c.)
c 1.9 Does the facility discharge to a receiving water that flows through Indian Country?
c 0 Yes ElNo
1.10 Provide design and actual flow rates in the designated spaces. Design Flow Rate
0.5 mgd
- Annual Average Flow Rates(Actual)
Two Years Ago Last Year This Year
co
-0 rec 3 0.10 0.11
c _o mgd On mgd mgd
"- Maximum Daily Flow Rates(Actual)
o Two Years Ago Last Year This Year
0.17 mgd 0.16 mgd 0.14 mgd
co 1.11 Provide the total number of effluent discharge points to waters of the United States by type.
o Total Number of Effluent Discharge Points by Type
d a Constructed
a'1— Treated Effluent Untreated Effluent Combined Sewer Bypasses Emergency
cs a Over
flows Overflows
0
to
C 1 N/A N/A N/A N/A
EPA Form 3510-2A(Revised 3-19) Page 2

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
Outfalls Other Than to Waters of the United States
1.12 Does the POTW discharge wastewater to basins,ponds,or other surface impoundments that do not have outlets for
discharge to waters of the United States?
❑ Yes ElNo 4 SKIP to Item 1.14.
1.13 Provide the location of each surface impoundment and associated discharge information in the table below.
Surface Impoundment Location and Discharge Data
Average Daily Volume Continuous or Intermittent
Location Discharged to Surface
Impoundment (check one)
❑ Continuous
gpd ❑ Intermittent
❑ Continuous
gpd ❑ Intermittent
O Continuous
y gpd ❑ Intermittent
. 1.14 Is wastewater applied to land?
❑ Yes ❑✓ No 4 SKIP to Item 1.16.
O 1.15 Provide the land application site and discharge data requested below.
Land Application Site and Discharge Data
co
o Continuous or
Location Size Average Daily Volume Intermittent
o' Applied (check one)
acres d ❑ Continuous
o gp ❑ Intermittent
acres d 0 Continuous
o gp ❑ Intermittent
-a 0 Continuous
acres gpd 0 Intermittent
T. 1.16 Is effluent transported to another facility for treatment prior to discharge?
o ❑ Yes ❑✓ No 4 SKIP to Item 1.21.
1.17 Describe the means by which the effluent is transported(e.g.,tank truck,pipe).
1.18 Is the effluent transported by a party other than the applicant?
❑ Yes ❑ No 4 SKIP to Item 1.20.
1.19 Provide information on the transporter below.
Transporter Data
Entity name Mailing address(street or P.O.box)
City or town State ZIP code
Contact name(first and last) Title
Phone number Email address
EPA Form 3510-2A(Revised 3-19) Page 3

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
1.20 In the table below,indicate the name,address,contact information,NPDES number,and average daily flow rate of the
receiving facility.
Receiving Facility Data
-a Facility name Mailing address(street or P.O.box)
City or town State ZIP code
0
t.)
Contact name(first and last) Title
0
Phone number Email address
flNPDES number of receiving facility(if any) ❑None Average daily flow rate mgd
cn
0 1.21 Is the wastewater disposed of in a manner other than those already mentioned in Items 1.14 through 1.21 that do not
have outlets to waters of the United States(e.g.,underground percolation,underground injection)?
12)
r ❑ Yes ❑✓ No 4 SKIP to Item 1.23.
c 1.22 Provide information in the table below on these other disposal methods.
Information on Other Disposal Methods
o Disposal Location of Size of Annual Average Continuous or Intermittent
-a Method Daily Discharge
R Description Disposal Site Disposal Site Volume (check one)
acresgpd 0 Continuous
0 Intermittent
0
acresgpd 0 Continuous
❑ Intermittent
acresgpd ❑ Continuous
❑ Intermittent
1.23 Do you intend to request or renew one or more of the variances authorized at 40 CFR 122.21(n)?(Check all that apply.
d w Consult with your NPDES permitting authority to determine what information needs to be submitted and when.)
a ❑ Discharges into marine waters(CWA ❑ Water quality related effluent limitation(CWA Section
cr
at et Section 301(h)) 302(b)(2))
❑✓ Not applicable
1.24 Are any operational or maintenance aspects(related to wastewater treatment and effluent quality)of the treatment works
the responsibility of a contractor?
❑ Yes ❑ No+SKIP to Section 2.
1.25 Provide location and contact information for each contractor in addition to a description of the contractor's operational
and maintenance responsibilities.
Contractor Information
Contractor 1 Contractor 2 Contractor 3
c Contractor name
(company name)
Mailing address
(street or P.O.box)
City,state,and ZIP
code
Contact name(first and
0 last)
Phone number
Email address
Operational and
maintenance
responsibilities of
contractor
EPA Form 3510-2A(Revised 3-19) Page 4

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
SECTION 2.ADDITIONAL INFORMATION(40 CFR 122.21(j)(1)and(2))
o Outfalls to Waters of the United States
2.1 Does the treatment works have a design flow greater than or equal to 0.1 mgd?
o ✓❑ Yes 0 No 4 SKIP to Section 3.
0 2.2 Provide the treatment works'current average daily volume of inflow Average Daily Volume of Inflow and Infiltration
;) and infiltration.
1085 gpd
Indicate the steps the facility is taking to minimize inflow and infiltration.
Visual Observations for defects
0
2.3 Have you attached a topographic map to this application that contains all the required information?(See instructions for
R 0
0 specific requirements.)
a,2
✓❑ Yes ❑ No
E 2.4 Have you attached a process flow diagram or schematic to this application that contains all the required information?
3 (2 (See instructions for specific requirements.)
a a,
o ❑✓ Yes 0 No
2.5 Are improvements to the facility scheduled?
❑ Yes ✓❑ No 4 SKIP to Section 3.
Briefly list and describe the scheduled improvements.
0
1.
E
a 2.
E
co
w
0
3.
s 4.
co
g 2.6 Provide scheduled or actual dates of completion for improvements.
Scheduled or Actual Dates of Completion for Improvements
E Affected Attainment of
Scheduled Begin End Begin
Outfalls Operational
o Improvement Construction Construction Discharge
Q. (from above) (list outfall (MM/DD/YYYY) (MM/DD/YYYY) (MM/DD/YYYY) Level
cu
number) (MM/DD/YYYY)
cu
.
d
U 2.
c
3.
4.
2.7 Have appropriate permits/clearances conceming other federal/state requirements been obtained?Briefly explain your
response.
❑ Yes 0 No ❑ None required or applicable
Explanation:
EPA Form 3510-2A(Revised 3-19) Page 5

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
SECTION 3.INFORMATION ON EFFLUENT DISCHARGES(40 CFR 122.21(j)(3)to(5))
3.1 Provide the following information for each outfall.(Attach additional sheets if you have more than three outfalls.)
Outfall Number o01 Outfall Number Outfall Number
State North Carolina
New Hanover
County
w
City or town Wilmington
Distance from shore 160 ft. ft. ft.
Q
Depth below surface 15 ft. ft. ft.
Average daily flow rate 0.12 mgd mgd mgd
Latitude 34° 19' 26" °
Longitude 78° 00' 50" "
3.2 Do any of the outfalls described under Item 3.1 have seasonal or periodic discharges?
o ❑ Yes ✓❑ No 4 SKIP to Item 3.4.
R 3.3 If so,provide the following information for each applicable outfall.
r
Outfall Number Outfall Number Outfall Number
7.0
Number of times per year
o discharge occurs
a Average duration of each
discharge(specify units)
c Average flow of each mgd mgd mgd
codischarge
Months in which discharge
occurs
3.4 Are any of the outfalls listed under Item 3.1 equipped with a diffuser?
❑✓ Yes ❑ No 4 SKIP to Item 3.6.
3.5 Briefly describe the diffuser type at each applicable outfall.
O.
Outfall Number 001 Outfall Number Outfall Number
d
VJ
9 diffuser nozzles on
approximately 8'centers
o ui 3.6 Does the treatment works discharge or plan to discharge wastewater to waters of the United States from one or more
discharge points?
g w ❑✓ Yes ❑ No 4SKIP to Section 6.
EPA Form 3510-2A(Revised 3-19) Page 6

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
3.7 Provide the receiving water and related information(if known)for each outfall.
Outfall Number 001 Outfall Number Outfall Number
Receiving water name Cape Fear River
Name of watershed,river, Indian Creek
o or stream system
C2- U.S.Soil Conservation
y Service 14-digit watershed 030300050501
o code
Name of state Cape Fear River Basin
management/river basin
rn
U.S.Geological Survey
8-digit hydrologic 03030005
42)
cataloging unit code
Critical low flow(acute) cfs cfs cfs
Critical low flow(chronic) cfs cfs cfs
Total hardness at critical mg/L of mg/L of mg/L of
low flow CaCO3 CaCO3 CaCO3
3.8 Provide the following information describing the treatment provided for discharges from each outfall.
Outfall Number o01 Outfall Number Outfall Number
Highest Level of 0 Primary 0 Primary ❑ Primary
Treatment(check all that 0 Equivalent to 0 Equivalent to ❑ Equivalent to
apply per outfall) secondary secondary secondary
0 Secondary 0 Secondary 0 Secondary
0 Advanced 0 Advanced 0 Advanced
0 Other(specify) 0 Other(specify) 0 Other(specify)
0
0
Design Removal Rates by
.r_ Outfall
U,
BOD5 or CBOD5 85 %
1°i TSS 85 % ok
l Not applicable 0 Not applicable 0 Not applicable
Phosphorus %
® Not applicable 0 Not applicable 0 Not applicable
Nitrogen
Other(specify) VI Not applicable 0 Not applicable 0 Not applicable
EPA Form 3510-2A(Revised 3-19) Page 7

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
3.9 Describe the type of disinfection used for the effluent from each outfall in the table below. If disinfection varies by
season,describe below.
._
c Outfall Number 001 Outfall Number Outfall Number
Disinfection type UV
C)
Seasons used 4 seasons
Dechlorination used? ✓❑ Not applicable ❑ Not applicable ❑ Not applicable
❑ Yes ❑ Yes ❑ Yes
❑ No ❑ No El No
3.10 Have you completed monitoring for all Table A parameters and attached the results to the application package?
✓❑ Yes ❑ No
3.11 Have you conducted any WET tests during the 4.5 years prior to the date of the application on any of the facility's
discharges or on any receiving water near the discharge points?
❑✓ Yes ❑ No 4 SKIP to Item 3.13.
3.12 Indicate the number of acute and chronic WET tests conducted since the last permit reissuance of the facility's
discharges by outfall number or of the receiving water near the discharge points.
Outfall Number 001 Outfall Number Outfall Number
Acute Chronic Acute Chronic Acute Chronic
Number of tests of discharge 12
water
Number of tests of receiving
water
3.13 Does the treatment works have a design flow greater than or equal to 0.1 mgd?
,� ❑✓ Yes ❑ No 4 SKIP to Item 3.16.
3.14 Does the POTW use chlorine for disinfection,use chlorine elsewhere in the treatment process,or otherwise have
reasonable potential to discharge chlorine in its effluent?
❑ Yes 4 Complete Table B,including chlorine. ✓❑ No 4 Complete Table B,omitting chlorine.
= 3.15 Have you completed monitoring for all applicable Table B pollutants and attached the results to this application
package?
❑✓ Yes ❑ No
3.16 Does one or more of the following conditions apply?
• The facility has a design flow greater than or equal to 1 mgd.
• The POTW has an approved pretreatment program or is required to develop such a program.
• The NPDES permitting authority has informed the POTW that it must sample for the parameters in Table C,must
sample other additional parameters(Table D),or submit the results of WET tests for acute or chronic toxicity for
each of its discharge outfalls(Table E).
Yes 4 Complete Tables C,D,and E as
❑ applicable. IDNo SKIP to Section 4.
3.17 Have you completed monitoring for all applicable Table C pollutants and attached the results to this application
package?
Yes ❑ No
3.18 Have you completed monitoring for all applicable Table D pollutants required by your NPDES permitting authority and
attached the results to this application package?
❑✓ Yes ❑ No additional sampling required by NPDES
permitting authority.
EPA Form 3510-2A(Revised 3-19) Page 8

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
3.19 Has the POTW conducted either(1)minimum of four quarterly WET tests for one year preceding this permit application
or(2)at least four annual WET tests in the past 4.5 years?
El Yes ❑ No 4 Complete tests and Table E and SKIP to
Item 3.26.
3.20 Have you previously submitted the results of the above tests to your NPDES permitting authority?
El Yes ❑ No 4 Provide results in Table E and SKIP to
Item 3.26.
3.21 Indicate the dates the data were submitted to your NPDES permitting authority and provide a summary of the results.
Date(s)Submitted Summary of Results
(MM/DD/YYYY)
all results passed from 2018 to 2020
Submitted thru eDMR&Aquatic Toxicology Branch/Division of
1/22/18-7/24/20 Water Resources @ DEQ
O
R 3.22 Regardless of how you provided your WET testing data to the NPDES permitting authority,did any of the tests result in
toxicity?
❑ Yes ✓❑ No 4 SKIP to Item 3.26.
u3.23 Describe the cause(s)of the toxicity:
d
w
3.24 Has the treatment works conducted a toxicity reduction evaluation?
❑ Yes ❑ No 4 SKIP to Item 3.26.
3.25 Provide details of any toxicity reduction evaluations conducted.
3.26 Have you completed Table E for all applicable outfalls and attached the results to the application package?
ID Yes Not applicable because previously submitted
information to the NPDES 'ermittin' authori .
SECTION 4. INDUSTRIAL DISCHARGES AND HAZARDOUS WASTES(40 CFR 122.21(j)(6)and(7))
4.1 Does the POTW receive discharges from SIUs or NSCIUs?
✓❑ Yes ❑ No 4 SKIP to Item 4.7.
4.2 Indicate the number of SIUs and NSCIUs that discharge to the POTW.
Number of SIUs Number of NSCIUs
y 1
0
R 4.3 Does the POTW have an approved pretreatment program?
❑ Yes ❑✓ No
R 4.4 Have you submitted either of the following to the NPDES permitting authority that contains information substantially
identical to that required in Table F:(1)a pretreatment program annual report submitted within one year of the
application or(2)a pretreatment program?
❑ Yes ✓❑ No 4 SKIP to Item 4.6.
R 4.5 Identify the title and date of the annual report or pretreatment program referenced in Item 4.4.SKIP to Item 4.7.
U)
4.6 Have you completed and attached Table F to this application package?
✓❑ Yes ❑ No
EPA Form 3510-2A(Revised 3-19) Page 9

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
4.7 Does the POTW receive,or has it been notified that it will receive,by truck,rail,or dedicated pipe,any wastes that are
regulated as RCRA hazardous wastes pursuant to 40 CFR 261?
❑ Yes ✓❑ No 4 SKIP to Item 4.9.
4.8 If yes,provide the following information:
Annual
Hazardous Waste Waste Transport Method Amount of Units
Number (check all that apply) Waste
Received
❑ Truck ❑ Rail
C ❑ Dedicated pipe ❑ Other(specify)
❑ Truck ❑ Rail
❑ Dedicated pipe ❑ Other(specify)
N ❑ Truck ❑ Rail
❑ Dedicated pipe ❑ Other(specify)
I R
N
4.9 Does the POTW receive,or has it been notified that it will receive,wastewaters that originate from remedial activities,
including those undertaken pursuant to CERCLA and Sections 3004(7)or 3008(h)of RCRA?
0
0 Yes ❑✓ No 4 SKIP to Section 5.
% 4.10 Does the POTW receive(or expect to receive)less than 15 kilograms per month of non-acute hazardous wastes as
specified in 40 CFR 261.30(d)and 261.33(e)?
❑ Yes 4 SKIP to Section 5. 0 No
4.11 Have you reported the following information in an attachment to this application:identification and description of the
site(s)or facility(ies)at which the wastewater originates;the identities of the wastewater's hazardous constituents;and
the extent of treatment,if any,the wastewater receives or will receive before entering the POTW?
0 Ye
s El No
SECTION 5.COMBINED SEWER OVERFLOWS(40 CFR 122.21(j)(8))
5.1 Does the treatment works have a combined sewer system?
c� ❑ Yes ❑' No 4SKIP to Section 6.
cts
5.2 Have you attached a CSO system map to this application?(See instructions for map requirements.)
a 0 Yes 0 No
5.3 Have you attached a CSO system diagram to this application?(See instructions for diagram requirements.)
c`ni ❑ Yes ❑ No
EPA Form 3510-2A(Revised 3-19) Page 10

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
5.4 For each CSO outfall,provide the following information.(Attach additional sheets as necessary.)
CSO Outfall Number CSO Outfall Number CSO Outfall Number
c City or town
0
o. State and ZIP code
o
to 1
o County
0
° °
o Latitude °
o rn °
Longitude "
Distance from shore ft. ft. ft.
Depth below surface ft. ft. ft.
5.5 Did the POTW monitor any of the following items in the past year for its CSO outfalls?
CSO Outfall Number CSO Outfall Number CSO Outfall Number
Rainfall ❑ Yes ❑No ❑ Yes ❑ No ❑ Yes ❑ No
0)
c
•o CSO flow volume ❑ Yes ❑No 0 Yes 0 No 0 Yes ❑ No
CSO pollutant ❑ Yes ❑No 0 Yes 0 No ❑ Yes ❑ No
o concentrations
c) Receiving water quality ❑ Yes ❑No ❑ Yes 0 No 0 Yes 0 No
CSO frequency ❑ Yes ❑No 0 Yes 0 No 0 Yes ❑ No
Number of storm events ❑ Yes ❑No ❑ Yes 0 No ❑ Yes ❑ No
5.6 Provide the following information for each of your CSO outfalls.
CSO Outfall Number CSO Outfall Number CSO Outfall Number
co
Number of CSO events in events events events
y the past year
co
a
c Average duration per hours hours hours
c
event 0 Actual or 0 Estimated 0 Actual or 0 Estimated 0 Actual or 0 Estimated
a)
Li' million gallons million gallons million gallons
o Average volume per event
Cn
6-) 0 Actual or 0 Estimated 0 Actual or 0 Estimated 0 Actual or 0 Estimated
Minimum rainfall causing inches of rainfall inches of rainfall inches of rainfall
a CSO event in last year 0 Actual or 0 Estimated 0 Actual or 0 Estimated 0 Actual or 0 Estimated
EPA Form 3510-2A(Revised 3-19) Page 11

EPA Identification Number NPDES Permit Number Facility Name Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF OMB No.2040-0004
5.7 Provide the information in the table below for each of your CSO outfalls.
CSO Outfall Number CSO Outfall Number CSO Outfall Number
Receiving water name
Name of watershed/
w stream system
w U.S.Soil Conservation ❑ Unknown 0 Unknown 0 Unknown
Service 14-digit
c watershed code
: (if known)
d Name of state
ce management/river basin
NU.S.Geological Survey 0 Unknown 0 Unknown 0 Unknown
8-Digit Hydrologic Unit
Code(if known)
Description of known
water quality impacts on
receiving stream by CSO
(see instructions for
exam'les
SECTION 6.CHECKLIST AND CERTIFICATION STATEMENT(40 CFR 122.22(a)and(d))
6.1 In Column 1 below, mark the sections of Form 2A that you have completed and are submitting with your application. For
each section,specify in Column 2 any attachments that you are enclosing to alert the permitting authority.Note that not
all applicants are required to provide attachments.
Column 1 Column 2
▪ Section 1:Basic Application
Information for All Applicants ❑ w/variance request(s) El w/additional attachments
❑ Section 2:Additional El w/topographic map El w/process flow diagram
Information El w/additional attachments
El w/Table A El w/Table D
❑ Section 3:Information on El w/Table B ❑ w/Table E
;= Effluent Discharges
E El w/Table C ❑✓ w/additional attachments
Section 4:Industrial El w/SIU and NSCIU attachments 0 w/Table F
N El Discharges and Hazardous
c Wastes El w/additional attachments
0 ❑ Section 5:Combined Sewer 0 w/CSO map 0 w/additional attachments
d Overflows ❑ w/CSO system diagram
U
Section 6:Checklist and
R ❑ Certification Statement El w/attachments
Y 6.2 Certification Statement
U
d
v /certify under penalty of law that this document and all attachments were prepared under my direction or supervision in 1
accordance with a system designed to assure that qualified personnel properly gather and evaluate the information
submitted.Based on my inquiry of the person or persons who manage the system,or those persons directly responsible
for gathering the information,the information submitted is,to the best of my knowledge and belief,true,accurate,and
complete.I am aware that there are significant penalties for submitting false information,including the possibility of fine
1 and imprisonment for knowing violations.
(prin or type first and last name) Official title
w • V.
ate si
, Cc_ _._...) ---.
lcA(C{
EPA Form 3510-2A(Revised 3-19) Page 12

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
110043452475 NC0081736 US421 WWTF 001 OMBNo.2040-0004
TABLE A.EFFLUENT PARAMETERS FOR ALL POTWS
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of Method' (include units)
Value Units Value Units Sam les
Biochemical oxygen demand
l BOD5 or 0 CBOD5 14 mg/L 5 mg/L 161 SM5210B 2 mg/L O MDL
resort one
Fecal coliform 59 cfu/100 mL 2 cfu/100 mL 158 Colilert 18 1 cfu/100 El ML
O MDL
Design flow rate 0.16 mgd 0.11 mgd 365
pH(minimum) 6 s.u.
pH(maximum) 7 S.U.
Temperature(winter) 22 degrees Celsius 17 degrees Celsius 67
Temperature(summer) 33 degrees Celsius 26 degrees Celsius 80
Total suspended solids(TSS) 1s mg/L 7 mg/L 171 SM2540D 2 mg/L p MDL
I Sampling shall be conducted according to sufficiently sensitive test procedures(i.e.,methods)approved under 40 CFR 136 for the analysis of pollutants or pollutant parameters or
required under 40 CFR chapter I,subchapter N or 0.See instructions and 40 CFR 122.21(e)(3).
EPA Form 3510-2A(Revised 3-19) Page 13

This page intentionally left blank.

EPA Identification Number NPDES Period Number Facility Name Outlall Number Form Approved 03/05/19
110043452475 NC0081736 US 421 W WTF 001 OMB No.2040-0004
TABLE B.EFFLUENT PARAMETERS FOR ALL POTWS WITH A FLOW EQUAL TO OR GREATER THAN 0.1 MGD
Maximum Daily Discharge Average Daily Discharge
Analytical ML or MDL 1
Pollutant Value Units Value Units Number of Method') (indude units)
Samples
o ML
Ammonia(as N) 4 mg/L <1 mg/L 165 EPA 350.1.2 1 mg/L o MDL
Chlorine N/A ❑ML
(total residual,TRC)2 ❑MDL
0 ML
Dissolved oxygen 9 mg/L 7 mg/L 160 SM 4500-0•G 5 mg/L o MDL
Nitrate/nitrite 7.6 mg/L 0.3 mg/L 4 EPA 353.2 0.02 mg/L o MDL
0 ML
Kjeldahl nitrogen 13.9 mg/L 1.9 mg/L 4 EPA 351.2 0.5 mg/L o MDL
0 ML
Oil and grease 11 mg/L <5 mg/L 158 EPA 1664 5 mg/L o MDL
Phosphorus 0.09 mg/L <0.05 mg/L 4 Hach 8190-5 0.05 mg/L o MDL
o ML
Total dissolved solids 1340 mg/L 739 mg/L 2 SM 2540 C 2.5 mg/L o MDL
I Sampling shall be conducted according to sufficiently sensitive test procedures(i.e.,methods)approved under 40 CFR 136 for the analysis of pollutants or pollutant parameters or
required under 40 CFR chapter I,subchapter N or 0.See instructions and 40 CFR 122.21(e)(3).
2 Facilities that do not use chlorine for disinfection,do not use chlorine elsewhere in the treatment process,and have no reasonable potential to discharge chlorine in their effluent are not
required to report data for chlorine.
EPA Form 3510-2A(Revised 3-19) Page 15

This page intentionally left blank.

EPA Identficaion Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF 001 OMB No.2040-0004
TABLE C.EFFLUENT PARAMETERS FOR SELECTED POTWS
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of Method' (include units)
Value Units Value Units Samples
Metals,Cyanide,and Total Phenols
Hardness(as CaCO3) 52 mg/L 34 mg/L 6 SM 2340-C 0 mg/L O MDL
Antimony,total recoverable <0.01 mg/L <0.01 mg/L 2 EPA 200.7 0.01 mg/L O MDL
0 ML
Arsenic,total recoverable <0.01 mg/L <0.01 mg/L 2 EPA 200.7 0.01 mg/L o MDL
CI ML
Beryllium,total recoverable <0.01 mg/L <0.01 mg/L 2 EPA 200.7 0.01 mg/L O MDL
CI ML
Cadmium,total recoverable <0.01 mg/L <0.01 mg/L 2 EPA 200.7 0.01 mg/L D MDL
ID ML
Chromium,total recoverable <0.01 mg/L <0.01 mg/L 2 EPA 200.7 0.01 mg/L D MDL
0 ML
Copper,total recoverable <0.01 mg/L <0.01 mg/L 2 EPA 200.7 0.01 mg/L D MDL
Lead,total recoverable <0.01 mg/L <0.01 mg/L 2 EPA 200.7 0.01 mg/L o MDL
Mercury,total recoverable <0.500 ng/L <0.500 ng/L 2 EPA 1631e 0.50 mg/L ML
O MDL
0 ML
Nickel,total recoverable <o.01 mg/L <0.01 mg/L 2 EPA 200.7 0.01 mg/L O MDL
0 ML
Selenium,total recoverable <0.01 mg/L <0.01 mg/L 2 EPA 200.7 0.01 mg/L O MDL
la AIL
Silver,total recoverable <0.01 mg/L <0.01 mg/L 2 EPA 200.7 0.01 mg/L D MDL
Thallium,total recoverable <0.01 mg/L. <0.01 mg/L 2 EPA 200.7 0.01 mg/L o MDL
Zinc,total recoverable 0.013 mg/L 0.013 mg/L 2 EPA 200.7 0.01 mg/L 13 MI-
o MDL
0 ML
Cyanide <0.005 mg/L <0.005 mg/L 2 EPA 335.4 .005 mg/L O MDL
0 ML
Total phenolic compounds 0.010 mg/L <0.005 mg/L 2 EPA 420.1 .005 mg/L O MDL
Volatile Organic Compounds
Acrolein <5 Erg/L <5 pg/L 2 EPA 624 5 /L El ML
pg O MDL
Acrylonitrile <5 pg/L <5 RA EPA 624 5 pg/L ML
0 MDL
Benzene <0.5 pg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
0 ML
Bromoform <0.5 pg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
EPA Form 3510-2A(Revised 3-19) Page 17

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
110043452475 NC0081736 US 421 W WTF 001 OMB No.2040-0004
TABLE C.EFFLUENT PARAMETERS FOR SELECTED POTWS
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of Method' (include units)
Value Units Value Units Samples
ML
Carbon tetrachloride <0.5 µg/L <0.5 pg/L 2 EPA 624 0.5 pg/L 0 MDL
Chlorobenzene <0.5 pg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
Chlorodibromomethane <0.5 pg/L <0.5 µg/L 2 EPA 624 0.5 pg/L O MDL
Chloroethane <0.5 µg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
0 ML
2-chloroethyhrinyl ether <5 µg/L <5 pg/L 2 EPA 624 5 pg/L O MDL
0 ML
Chloroform <0.5 pg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
Dichlorobromomethane <0.5 µg/L <0.5 µg/L 2 EPA 624 0.5 pg/L O MDL
ML
1,1-dichloroethane <0.5 µg/L <0.5 µg/L 2 EPA 624 0.5 pg/L O MDL
1,2-dichloroethane <0.5 µg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
El ML
trans-1,2-dichloroethylene <0.5 µg/L <0.5 µg/L 2 EPA 624 0.5 pg/L O MDL
1,1-dichloroethylene <0.5 µg/L <0.5 pg/L 2 EPA 624 0.5 pg/L OMDL
1,2-dichloropropane <0.5 pg/L <0.5 µg/L 2 EPA 624 0.5 pg/L O MDL
1,3-dichloropropylene <0.5 µg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
Ethylbenzene <0.5 pg/L <0.5 pg/L 2 EPA 624 0.5 pg/L 1:1O MDL
Methyl bromide <0.5 µg/L <0.5 pg/L 2 EPA 624 0.5 pg/L p MDL
Methyl chloride <0.5 pg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
Methylene chloride <0.5 µg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
1,1,2,2-tetrachloroethane <0.5 µg/L <0.5 pg/L 2 EPA624 0.5 pg/L OMDL
Tetrachloroethylene <0.5 pg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
Toluene <0.5 pg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O+MDL
0 ML
1,1,1-lchloroethane <0.5 pg/L <0.5 pg/L 2 EPA 624 0.5 pg/L O MDL
0 ML
1,1,2-dichloroethane <0.5 will <0.5 pg/L 2 EPA 624 0.5 pg/L OMDL
EPA Form 3510-2A(Revised 3-19) Page 18

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H

EPA Identification Number NPDES Permit Number Facility Name Outtall Number Form Approved 03/05/19
110043452475 NC0081736 US 421 WWTF 001 OMB No.2040-0004
TABLE C.EFFLUENT PARAMETERS FOR SELECTED POTWS
Maximum Daily Discharge Average Daily Discharge Analytical ML or MDL
Pollutant Number of Method
Value Units Value Units (include units)
Samples
o ML
1,2-diphenylhydrazine <5 pg/L <5 pg/L 2 EPA 625 5 PO-0 MDL
Fluoranthene <5 pg/L <5 pg/L 2 EPA 625 5 p9/L O ML
❑MDL
O ML
Fluorene <5 pg/L <5 pg/L 2 EPA 625 5 pg/L 0 MDL
Hexachlorobenzene <5 pg/L <5 pg/L 2 EPA 625 5 /L 0 ML
N9 ❑MDL
0 ML
Hexachlorobutadiene <5 pg/L <5 pg/L 2 EPA 625 5 p9/L 0 MDL
Hexachlorocyclo-pentadiene <25 pg/L <25 pg/L 2 EPA 625 25 pg/L 0 ML
❑MDL
0 ML
Hexachloroethane <5 pg/L <5 pg/L 2 EPA 625 5 pg/L 0 MDL
0 ML
Indeno(1,2,3-cd)pyrene <5 pg/L <5 pg/L 2 EPA 625 5 pglL 0 MDL
Isophorone <5 pg/L <5 pg/L 2 EPA 625 5 pg/L o MDL
0 ML
Naphthalene <5 pg/L <5 pg/L 2 EPA 625 5 pg/L 0 MDL
Nitrobenzene <5 pg/L <5 pg/L 2 EPA 625 5 N9/L ML
❑MDL
0 ML
N-nitrosodi-n-propylamine <5 pg/L <5 pg/L 2 EPA 625 5 pg/L 0 MDL
ML
N-nitrosodimethylamine <5 pg/L <5 pg/L 2 EPA 625 5 pg/L 0 MDL
N-nitrosodiphenylamine <5 pg/L <5 pg/L 2 EPA 625 5 pg/L 0 ML
❑MDL
Phenanthrene <5 pg/L <5 pg/L 2 EPA 625 5 pg/L ML
❑MDL
Pyrene <5 pg/L <5 pg/L 2 EPA 625 5 pg/L 0 MDL
0 ML
1,2,4-trichlorobenzene <5 pg/L <5 pg/L 2 EPA 625 5 pg/L 0 MDL
I Sampling shall be conducted according to sufficiently sensitive test procedures(i.e.,methods)approved under 40 CFR 136 for the analysis of pollutants or pollutant parameters or
required under 40 CFR Chapter I,Subchapter Nor O.See instructions and 40 CFR 122.21(e)(3).
EPA Form 3510-2A(Revised 3-19) Page 21

This page intentionally left blank.

EPA Identification Number NPDES Permit Number Facility Name Outran Number Form Approved 03/05/19
110043452475 NC0081736 US 421 W WTF 001 OMB No.2040-0004
TABLE E.EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample.Copy the table to report additional test results.
Test Information
Test Number 1 Test Number 2 Test Number 3
Test species Pimephales promelas Pimephales promelas Pimephales promelas
Age at initiation of test 5-6 days 5-6 days 5-6 days
Outfall number 001 001 001
Date sample collected 03/07/2019 06/05/2019 09/19/2019
Date test started 03/08/2019 06/06/2019 09/20/2019
Duration 24-hours 24-hours 24-hours
Toxicity Test Methods
Test method number EPA 2000.0 EPA 2000.0 EPA 2000.0
Manual title EPA-821-R-02-012 EPA-821-R-02-012 EPA-821-R-02-012
Edition number and year of publication Fifth Edition,October 2002 Fifth Edition,October 2002 Fifth Edition,October 2002
Page number(s) 1-266 1-266 1-266
Sample Type
Check one: ❑Grab ❑Grab ❑Grab
O 24-hour composite D 24-hour composite '❑24-hour composite
Sample Location
Check one: 0 Before Disinfection ❑ Before Disinfection 0 Before disinfection
❑After Disinfection ❑After Disinfection ❑After disinfection
O After Dechlorination ❑' After Dechlorination 0 After dechlorination
Point in Treatment Process
Describe the point in the treatment process Effluent Outfall 001,after all treatment Effluent Outfall 001,after all treatment Effluent Outfall 001,after all treatment
at which the sample was collected for each processes processes processes
test.
Toxicity Type
Indicate for each test whether the test was 0 Acute ❑' Acute El Acute
performed to asses acute or chronic toxicity,
or both.(Check one response) ❑Chronic El Chronic El Chronic
❑Both ❑Both ❑Both
EPA Form 3510-2A(Revised 3-19) Page 25

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
110043452475 NC0081736 US 421 W WTF 001 OMB No.2040-0004
' TABLE E.EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample.Copy the table to report additional test results.
Test Number 1 Test Number? Test Number 3
Test Type
Indicate the type of test performed.(Check one ❑� Static CI Static CI Static
response.)
❑Static-renewal Cl Static-renewal ❑Static-renewal
❑ Flow-through ❑ Flow-through ❑ Flow-through
Source of Dilution Water
Indicate the source of dilution water.(Check ❑✓ Laboratory water e❑ Laboratory water ✓❑ Laboratory water
one response.)
0 Receiving water 0 Receiving water ❑ Receiving water
If laboratory water,specify type. Soft Synthetic Water Soft Synthetic Water Soft Synthetic Water
If receiving water,specify source.
Type of Dilution Water
Indicate the type of dilution water.If salt Fresh water ❑✓ Fresh water CI Fresh water
water,specify"natural"or type of artificial
sea salts or brine used. ElSalt water(specify) ❑ Salt water(specify) ❑ Salt water(specify)
Percentage Effluent Used
Specify the percentage effluent used for all
concentrations in the test series. 0,90% 0,90% 0,90%
Parameters Tested
Check the parameters tested. r❑pH ❑Ammonia ❑✓ pH ❑Ammonia ❑' pH ❑Ammonia
❑Salinity ❑ Dissolved oxygen 0 Salinity ❑ Dissolved oxygen ❑Salinity ❑ Dissolved oxygen
0 Temperature ❑Temperature ❑Temperature
Acute Test Results
Percent survival in 100%effluent N/A % N/A % N/A
LCso N/A N/A N/A
95%confidence interval N/A % N/A °/° N/A %
Control percent survival 100 % 10o % l00 %
EPA Form 3510-2A(Revised 3-19) Page 26

EPA Identification Number NPDES Permit Number Facility Name Outfall Number Form Approved 03/05/19
110043452475 NC0081736 US 421 W WTF 001 OMB No.2040-0004
TABLE E.EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY
The table provides response space for one whole effluent toxicity sample.Copy the table to report additional test results.
Test Number 1 Test Number 2 Test Number 3
Acute Test Results Continued
Other(describe) Pass Pass Pass
1 Chronic Test Results
NOEC % % %
IC2s % % %
Control percent survival % % %
Other(describe)
Quality Control/Quality Assurance
Is reference toxicant data available? ❑✓ Yes ❑No ❑r Yes ❑No ID Yes 0 No
Was reference toxicant test within
acceptable bounds? 0 Yes El No ❑' Yes ❑No El Yes 0 No
What date was reference toxicant test run 03/05/2019 06/04/2019 10/20/0219
(MM/DD1 YYY)?
Other(describe) Under the Parameter tested it only let me Under the Parameter tested it only let me Under the Parameter tested it only let me
check one but we run. check one but we run. check one but we run.
pH,Temperature,and Dissolved Oxygen pH,Temperature,and Dissolved Oxygen pH,Temperature,and Dissolved Oxygen
EPA Form 3510-2A(Revised 3-19) Page 27

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Receiving Stream: Cape Fear River Sub-Basin: 03-06-17
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Stream Class: C-Swamp;PNA 8-digit HUG 03030005 Map not to scale

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Process Flow Narrative

The US 421 Wastewater Treatment Facility Process
Key components of the US 421 Wastewater Treatment Facility adaptive ecosystem treatment
process are aerated primary treatment tanks with attached growth media, also called moving
bed bioreactors (MBBRs). They are a commonly used technology to efficiently remove
carbonaceous material as a primary treatment step.
Three MBBRs are located within the facility. The attached growth media in these reactors, a
floating plastic substrate, is colonized by a community of bacteria in the form of a biofilm that
begins the biological treatment process. These reactors have coarse bubble diffusers that
provide mixing and supply the necessary oxygen to achieve optimum biofilm growth.
Submerged Fixed Film Reactors (SFFRs) follow the primary MBBRs. They reduce remaining BOD
to secondary levels and complete the nitrification process. The surface of the SFFR is covered
with vegetation supported on racks that are located inside a 3,000-SF glass hydroponic
greenhouse structure. These reactors are aerated with fine bubble diffusers, which provide the
oxygen required for treatment and keep the tank contents mixed.
In addition to the MBBRs and SFFRs, the facility includes two 30-ft diameter secondary
clarifiers, two cloth media filters, ultraviolet disinfection, an effluent pump station, a return
activated sludge/waste activated sludge (RAS/WAS) pump station, aerobic digesters, a sludge
loading station, and a screening and grit structure. Treated wastewater from the facility after
the UV Disinfection to a submerged diffuser to the Cape Fear River Basin through outfall 001.
WaterHub'Process Diagram, Pender County WWTP
GlassHouse
Structure
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Primary Equalization _ __
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UV
Clarifier Disc Filter Disinfection*
*Provided by others
Disposal

Toxicity Test Data
2020 for 2 different Species

ern. ,Ins. (C64)977-6$.42 fA) [phone]
P 0 Box 1E414 Greenvlle. SC 29606 4 Craftsman Cu.x. Gists,SC 29030
Pimephales promelas 24 Hr. Acute Pass/Fail Test
EPA-821-R-02-012 Method 2000
Client: PENDER COUNTY
Facility:WWTP 421
NPDES#:NC0081736
Test Date: 09-Sep-20
Laboratory ID#:T57340 APF
Test Reviewed and Approved By:
1/ /if...
Robert W.Kelley,Ph.D. Farhad Rostampour
QA/QC Officer Laboratory Director
Certification#E87819 SCDHEC Certification#23104
Test results presented in this report conform to all requirements of
NELAC,conducted under NELAC Certification Number E87819
Florida Dept.of Health.Included results pertain only to provided samples. NCDENR certification# 022
'age 1 of 4

r
Effluent Aquatic Toxicity Report Form -Acute Pass/Fail Date: 09/14/20
Facility Pender Count WWTP NPDES# NC0081736 Pipe# 001 County Pender
Laboratory Performing Test
X Comments
Signature of Operator in Responsible Charge
—
X
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Environmental Sciences Branch
Div.of Water Quality
N.C.DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
F Toxicity
North Carolina Acute Pass/Fail To c y Test
Collection Date: 9/8/20 Organism Tested:
Collection Time: 1000 Pimephales promelas
Test Start Date: 9/9/20
Sample Type/Duration Control 7.8 7.7
Grab Comp Duration pH Treatment 7.3 7.3
X 24 hr
--_ Control Sample start end
Hardness(mg/L) 82 Control 7.7 8.1
Spec.Cond.(pmhos) 319 2450 D.O. Treatment 8.4 7.4
Chlorine(mg/L) <0.05
Sample temp.at receipt 0.6
Mortality Replicate Mean Mortality
Treatment 1 (Control) A B C D
0% 0% 0% 0% 0%
Treatment 2(Exposure) A B C D
Concentration 90% 0% 0% 0% 0% 0%
Tested
(NOTE): If mean control mortality exceeds 10%,the test is considered invalid)
Calculate using
Arc-Sine Calculated Student's t N/A PASS X
SquareRoot
transformed
rmed Tabular Student's t 1.94 FAIL
data (ONE TAILED)
If the absolute value of the calculated t is less than or equal
to the absolute value of the tabular t, check PASS
If the absolute value of the calculated t is greater than the
absolute value of the tabular t, check FAIL.
If all vessels within each treatment have the same response
but the treatment two response is greater than the control, check FAIL.
DEM form AT-2(8/91)
Page 2 of 4

24 Hour Acute Toxicity Test
Test Method:EPA 821 R-02-012; Method 2000 Pimeshales peromelas
Client: TENDER COUNTY Sample ID:tFFLUENT Lab ID#: T57340 APF
Start Date: 09/09/20 Time: 01:30 PM Set By: AM
End Date: 09/10/20 Time: 01:49 PM Ended By: JZ
Test Vessel Test Solution Volume Incubator Transfer Volume Dilution Water
Fathead Minnows 500 mL plastic cup 200 mL #1 0.5 mL MHSF
Randomization pattern per SOP 'Neonates from common holding vessel $Light: 50-100 ft-c.16 hr light/8 hr dark
TEST ORGANISMS
for Ceriodaphnia dubiaipw
Comments:
Date Removed: Between: and: MHSF 8-28-20D
imephales promelas Source: ABS ays old: 6 Fish hatched 9-3-20 btw 1300-1430 MT
vsidopsis bahia Source: Days old:
Test Organisms Pre Fed b : AM Time: 1030
MORTALITY DATA
Cone. Initial S Cumulative Mortality Final Initial Final
Cond/ Cond/
Rep. organisms vnr +s nt r-hr +n nr Noiei., Temp D.O. pH Salini By: Temp D.O. pH Salini By:
A 10 0
Control B 10 0
C 10 0
D 10 0 0% 24.61 7.71 7.81 1.AM 24.61 8.11 7.71 IIZ
A 10 0
90.0% B 10 0
C 10 0
D 10 0 0% 24.41 8.41 7.31 I AM 24.61 7.41 7.31 11Z
A
B
C
D 1 I I 11 I
A
B
C
D 11 I 1 11 1 l
A
B
C
D I I I I 11 I 1
A
B
C
D I I 1 1 1 I I
A
B
C
D °C 1 mg/LI 1PPr 1 �c 1 n, iL.I ll'I'l I
Page 3 of 4
l

r
cnr ronenonta,Inc. (C64)077$b42 f A1' (ow 077 693©
PO Box 1E414 Creenvlle SC 29606 4 Go eel! SC 29650
Ceriodaphnia dubia 24 Hr. Acute Definitive Test
EPA-821-R-02-012 Method 2002
Client: PENDER COUNTY
Facility:WWTP 421
NPDES#:NC0081736
Test Date: 09-Sep-20
Laboratory ID#:T57340 C dubia
Test Reviewed and Approved By:
Robert W.Kelley,Ph.D. Farhad Rostampour
QA/QC Officer Laboratory Director
neac Certification#E87819 SCDHEC Certification#23104
Test results presented in this report conform to all requirements of
NELAC,conducted under NELAC Certification Number E87819
Florida Dept.of Health.Included results pertain only to provided sa-'les.ag e 1 of 4 NCDENR Certification# 022
I I

Effluent Toxicity Report Form-Chronic Pass/Fail and Acute LC50 Date 14-Sep-20
Facility:PENDER COUNTY WWTP NPDES#NC0081736 Pipe# 001 County Pender
Laboratory Performing Test: ETT Environmental, Inc. Comments
X
Signature of Operator in Responsible Charge
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch
Div.of Water Quality
N.C.DENR
1621 Mail Service Center
Raleigh,North Carolina 27699-1621
North Carolina Ceriodaohnia Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t=
Critical Value=
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 %Reduction=
#Young Produced %Mortality Avg.Reprod.
Adult (L)ive (D)ead
Control Control
Effluent%
Treatment 2 Treatment 2
Control CV
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
#Young Produced %3rd Brood PASS FAIL
Adult (L)ive (D)ead I I
Complete This for Either Test Test Start Date
Collection(Start)Date 09-Sep-20
pH 1st sample 1st sample 2nd sample Sample 1 07-Sep-20 Sample 2 NA
Control Sample Type(Duration).
Treatment 2 Grab Comp Duration 1st 2nd
Sample 1 , X 24hrs. Tox Tox
Sample 2 Dilution Sample Sample
start end start end start end
D.O. 1st sample 1st sale 2nd sample Hardness(mg/L) 82.0
Control Spec.Cond.(pmhos) 319 2450
Treatment 2 Chlorine(mg/L) <0.05 1
Sample Temp.at receipt(°C) 0.6
LC50/Acute Toxicity Test
(Mortality expressed as%,combining replicates)
0 6.25 12.5 25 50 100 Concentration
I
0 0 0 0 0 0 Mortality start/end start/end
LC50= >100 % Method of Determination 7.7 7.4 Control 8.1 8.1
95%Confidence Limits Moving Average Probit MI 6.8 7.6 High Conc. 8.8 7.9
NA% NA % Spearman Kerber Other X pH D.O.
Organism Tested Ceriodaphnia dubia I
DEM Form AT-1
Page 2 of 4
I t

24 Hour Acute Toxicity Test
Test Method:EPA 821 R-02-012 Method 2002 Ceriodaphnia dubia
Client: PENDER COUNTY Sample ID:PENDER COUNTY Lab ID#: T57340 C dub
Start Date: 09/09/20 Time: 01:00 PM Set By: JC
End Date: 09/10/20 Time: 01:31 PM Ended By: JZ
Test Vessel Test Solution Volume Incubator Transfer Volume Dilution Water
Daphnids 1 oz plastic cup 25 mL #1 0.05 mL MHSF
Randomization pattern per SOP 'Neonates from common holding vessel !Light: 50-100 ft-c.16 hr light/8 hr dark
TEST ORGANISMS
for Ceriodaphnia duhia k - Comments:
Date Removed: mown Between: 1700 and: 2200
imephales promelas Source: Days old: S-X 8/28,A-F 8/28
vsidopsis bahia Source: Days old: RANDOMIZED
Test Organisms Pre Fed b : JC Time: 11:00
MORTALITY DATA
Cone. initial g Cumulative Mortality Final
ii. IN Sand/ NIP! IIII
Rep. organisms a br a n. r n. ae 4, Mon.rp D.O. Salim D.O. Send
A 5 0
-
Control B 5 0
C 5 0
D 5 0 I. 0% 24.5 8.1 ®_aill 24.8 IMIOM ®
A 5 0
6.3% B 5 0
C 5 0
D 5 0 0% 24.5 8.3 7.6_®itaig 8.0®_®
A 5 0 r .
.12.5% B 5 0
C 5 0
D 5 0 0% 24.5 MI 7.6_EN'MIMI 7.9_®
A 5 0
25.0% B 5 0
C 5 0
D 5 0 I 0% 24.5 ®_01111 MIMI' 7.9_®
A 5 0
50.0% B 5 0
C 5 0
D 5 0 I 0% 24.5 8.4®_®WEIE111•311_®
A 5 0 _
100.0% B 5 0
C 5 0
D 5 0 I 0% 24.5 Ern 6.8_From Rap 7.9 7.6_®
A
B
C
D °C tral PPT _Mil FM PPT
Page 3 of 4