NC0083321_Report_20210104

ONWASA
onwasa.com
228 Georgetown Rd
Jacksonville. NC 28540
December 23, 2020
S. Daniel Smith, Director
Division of Water Resources, NCDEQ
1617 Mail Service Center
Raleigh, North Carolina 27699-1617 D © MOWED
ATTN: Brianna Young, Environmental Specialist PI -
RE: NPDES Permit No. NC0083321
Hubert Water Treatment Plant DIVISION OF WATER RESOURCES
DIRECTOR'S OFFICE
Grade ! Physical Chemical WPCS - -
Onslow Water and Sewer Authority, Onslow County
Director Smith:
report areprovided rin accordance with the requirements
This letter and enclosed
q
of Section A.(3) Discharge Alternatives Evaluation of the NPDES permit issued for the
above-referenced facility.
To help determine the underlying cause(s) for previous Whole Effluent Toxicity
(WET) test failures and the best alternative(s) to correct this situation, ONWASA entered
into a contract with W.K. Dickson & Company (Wilmington Office) earlier this year. They
partnered with an analytical laboratory (Shealy Consulting, LLC of Batesburg-Leesville,
SC) to establish sampling protocols, conduct sample analysis and provide a report on
their findings. A copy of that report is attached for your review.
As noted in Section 1.0 Executive Summary, Shealy Consulting worked with facility
staff to implement a comprehensive sampling program that included treatment lagoon
influent and effluent, as well as sediment samples taken at multiple locations. While
toxicity failures occurred in samples collected from the lagoon influent during ion
exchange resin process discharge, none of the final effluent or sediment samples
indicated any toxicity. Passing toxicity results were reported for the quarterly WET testing
done in June and September 2020.
Based on the study results where no acutely toxic final effluent samples could be
obtained and since passing NPDES testing for the second and third quarter of 2020,
further analysis of the potential discharge alternatives listed in Section A.(3) of the permit
has been suspended pending your review of this information and direction on how to
proceed. ONWASA is, however, proceeding with the report's recommendation to remove

I'
Page Two
December 23, 2020
solids from the lagoon system; a Request for Proposal to complete this work is currently
being advertised and we anticipate completion of all work by April 1, 2021.
Thank you for your consideration of this information and any guidance you can
provide to ensure ONWASA remains compliant with NPDES requirements. Please feel
free to contact me directly at [phone] or dmohronwasa.com you have any
additional questions.
Sincerely,
David M. Mohr, P.E.
Chief Operations Officer
Onslow Water and Sewer Authority
Encl.
C: Jeff Hudson, ONWASA Chief Executive Officer
Seth Brown, ONWASA Treatment Facilities Administrator
Carter Hubard, PE, W.K. Dickson and Company

SHEALY
TOXICITY IDENTIFICATION EVALUATION
ONSLOW WATER AND SEWER AUTHORITY
HUBERT WATER TREATMENT PLANT
NOVEMBER 2020
REPORT SUBMITTED TO:
W.K. DICKSON & CO., INC.
REPORT SUBMITTED BY:
Ac
Gam-- --�"
Laura S. davis
SHEALY CONSULTING, LLC.
343 W. Columbia Avenue
Batesburg-Leesville, South Carolina 29006

TABLE OF CONTENTS
SECTION TITLE PAGE
Section 1.0: Executive Summary 1
Section 2.0: Introduction 4
Section 3.0: Materials and Methods 6
3.1: Sample Collection, Handling, and Preservations 6
3.2: Sample Preparation 7
3.3: Toxicity Test Methodology 8
3.4: Analytical Procedures 11
Section 4.0: Results 12
4.1: Toxicity Test Results 12
4.2 Analytical Results 15
Section 5.0: Conclusions and Recommendations 16
Section 6.0: References 17
Appendix A: Site Map with Sampling Locations
Appendix B: August 11, 2020, Test Data
Appendix C: August 26, 2020, Test Data
Appendix D: August 31, 2020, Test Data
Appendix E: Analytical Results for Effluent Collected August 11, 2020
i

LIST OF TABLES
TABLE DESCRIPTION PAGE
Table 1: 2019-2020 Toxicity Test Results with Americamysis bahia 4
Table 2: Required Conditions for Acute Toxicity Tests with Americamysis bahia 9
Table 3: Test Methods for Analytical Procedures 11
Table 4: Toxicity Test Results for Samples Collected August 11, 2020 12
Table 5: Toxicity Test Results for Individual Grab Samples from Location #4
Collected August 11, 2020 13
Table 6: Toxicity Test Results for Samples Collected August 26, 2020 14
Table 7: Toxicity Test Results for Samples Collected August 31, 2020 14
Table 8: Analytical Characterization of Effluent Collected August 11, 2020 15

TOXICITY IDENTIFICATION EVALUATION FOR THE
ONSLOW WATER AND SEWER AUTHORITY
HUBERT WATER TREATMENT PLANT
NOVEMBER 2020 REPORT
SECTION 1.0 EXECUTIVE SUMMARY
The Onslow Water and Sewer Authority (ONWASA) of North Carolina operates the Hubert
Water Treatment Plant (WTP) which discharges wastewater into Queen Creek (permit
#NC0083321). The facility is required to conduct quarterly Whole Effluent Toxicity (WET)
toxicity tests with the species Americamysis bahia and has experienced five (5) failures of
quarterly tests conducted March 2019 to March 2020. The engineering firm of W.K. Dickson &
Co, Inc. (WKD) requested that Shealy Consulting, LLC (Shealy), conduct a Toxicity Identification
Evaluation (TIE) in response to these toxicity test failures.
On July 16, 2020 personnel from ONWASA, WKD and Shealy met at the Hubert WTP to tour the
facility and discuss the project. During that on-site visit, a thorough examination of the process
areas, chemicals used in the various processes and the three (3) treatment lagoons and one
overflow lagoon was made. It was also noted during the visit that the most recent toxicity test
for June 2020 resulted in a passing test, the first in many months. There was no apparent
reason for the passing test for June 2020. It was observed during the on-site visit that solids
were at very high levels in all three (3) lagoons, particularly lagoon #1 in which solids were all
the way to within inches of the surface. With this fact in mind the objective of initial portion of
the TIE was to evaluate the impact (if any) the solids had on the toxicity issue. This type of
environment with very high solids accumulations and low dissolved oxygen is ideal for the
formation of toxic organic selenides and hydrogen sulfide. Therefore, the first step of the TIE
HUBERT WTP TOXICITY IDENTIFICATION EVALUATION 1
SHEALY J

was to identify any areas of the lagoon system that could be contributing to final effluent
toxicity.
Three (3) Hubert WTP final effluent samples were collected and tested using non-compliance
process control procedures. This was performed in order to obtain a toxic sample on which to
perform toxicity characterization tests. The identification of toxic components in the effluent
requires fractionization of samples through a series of effluent manipulations. In this way, toxic
constituents of the effluent can be characterized and partitioned out into groups such as
oxidants, volatiles, non-polar organics, metal, etc. Phase I procedures are not toxicant specific
but are intended to establish whether a group of compounds are responsible for the observed
toxicity. In addition, grab samples of water entering and exiting (NPDES location) Lagoon #3
and entering Lagoon #1 (influent) were collected along with sediment samples from four (4)
different locations in Lagoons #1 and#2.
The toxicity tests on final effluent samples collected August 11, 26, and 31, 2020 all resulted in
passing toxicity tests. In fact, none of the final effluent samples indicated any toxicity
whatsoever. Grab water samples collected at entry and exit locations from Lagoon#3 also
passed toxicity. Sediment samples collected at four(4) locations in Lagoons #1 and #2
processed into elutriate tests following USEPA methods also indicated no toxicity whatsoever
which was not expected.
The only samples producing toxicity failures were collected at the influent entry into Lagoon 1
(labelled Location #4 on the sampling map located in the Appendices). Grab samples tested
from this location did indicate that toxicity was entering the lagoon system during the study,
even though toxicity was not observed in the final effluent. After the first set of samples were
collected on August 11, 2020, each time a sample was collected from location it was noted on
the chain of custody which water treatment process was discharging. Not surprisingly, the
toxicity for the influent samples varied significantly appearing to be associated with specific
water treatment process that was occurring during the time of sample collection. For instance,
the sampling conducted August 31, 2020 included two (2) samples collected from location #4
SHEALY HUBER I WTP TOXICITY IDEN I IFICATION EVALUATION 2

during the "Resin" process discharge into Lagoon #1, one test resulting in 10% mortality and
one test resulting in 100% mortality of the test organisms. Both grab samples collected on
August 26, 2020 at location#4 during the "resin" process discharge resulted in 100% mortality
to the test organisms. For the ten (10) samples collected at location#4 during the study the
only samples that were toxic were the ones collected during the "Resin" process discharge.
None of the samples labelled "backwash" or "no process started" were toxic.
As discussed previously, the toxicity tests on final effluent samples collected during this study
all resulted in passing tests. In addition, the NPDES test conducted for September 2020 also
resulted in a passing test. Since no acutely toxic final effluent samples could be obtained during
this study and since passing NPDES tests were observed for the second and third quarter of
2020, the TIE was terminated.
It is not known at this time what caused the toxicity at the Hubert WTP prior to June 2020, but
it appears that currently the lagoon system is removing the toxicity entering the system from
the water treatment process. It is also apparent from the testing on sediments samples
collected during the study that toxicity is not accumulating in the solids nor is it being
generated by the solids, at least not at the time of sample collection. However, it is
recommended that the solids be removed from the lagoon system as much as possible since
high solids accumulation such as what was observed during the July 2020 visit, will reduce
water retention time in the system and therefore treatment time. To ensure the best possible
results for aquatic toxicity testing it is beneficial to have as much treatment time as possible to
remove potentially toxic constituents entering the lagoon system. It is also recommended that
each time the Hubert WTP collects a sample for toxicity that a minimum of two (2) extra gallons
be collected and held in cold storage for TIE testing in the event of a future toxicity failure.
SHEALY .4) HUBERT WTP TOXICITY IDENTIFICATION EVALUATION

SECTION 2.0 INTRODUCTION
The Onslow Water and Sewer Authority of North Carolina operates the Hubert Water
Treatment Plant (WTP) which discharges wastewater into Queen Creek (permit#NC0083321).
The facility is required to conduct quarterly Whole Effluent Toxicity (WET)toxicity tests with the
species Americamysis bahia at a test concentration of 90%.
The facility has experienced five (5)failures of seven (7) toxicity tests conducted in 2019 and
2020. Table 1 provides toxicity test results for 2019-2020.
Table 1: 2019-2020 Toxicity Test Results with Americamysis bahia
Date % Mortality at 90% Result
Test Concentration
March 2019 60% Fail
June 2019 55% Fail
September 2019 80% Fail
December 2019 30% Fail
March 2020 60% Fail
June 2020 5% Pass
September 2020 0% Pass
The wastewater treatment facility consists of three (3) lagoons and one (1) overflow lagoon.
Wastewater from the WTP includes backwash from greensand and carbon filtration in addition
to reject wastewater from ion exchange units. The wastewater initially flows into Lagoon#1,
through Lagoon #2, and effluent is discharged after flowing through Lagoon #3. The maximum,
monthly average discharge is approximately 0.30 MGD. The effluent flows into Queen Creek,
classified as SA-HQW in the White Oak River Basin. A schematic of the WTP lagoon system is
available in Appendix A.
As a result of the toxicity failures, W.K. Dickson & Co., Inc., on behalf of the Onslow Water and
Sewer Authority, requested a Toxicity Identification Evaluation (TIE) to be conducted by Shealy
SHEA Y HUBER! WTP I OXICI FY IDENTIFICAI ION EVALUATION 4

Consulting, LLC. The first phase of the TIE plan included toxicity screening tests of grab water
samples and solids samples from specific locations throughout the lagoon system, as well as
final effluent composite samples as required in the NPDES permit (#NC0083321). This report
provides results of toxicity screening tests conducted August 2020.
SHEALY o HUBERT WTP TOXICITY IDENTIFICATION EVALUATION

SECTION 3.0 MATERIALS AND METHODS
3.1 SAMPLE COLLECTION, HANDLING, AND PRESERVATION
Water and solids samples were collected by personnel of Hubert WTP and were transported to
the testing laboratory by Shealy Consulting, LLC. Water samples were collected as composite or
grabs, according to the study plan designation at each location. Samples of solids were
collected from the bottom of the solids layer at each sampling location. Solids were collected in
glass amber jars.
Sample locations are designated on the schematic provided in Appendix A. Samples collected
on August 11, 2020, and August 26, 2020, included water and solids samples from the following
locations throughout the Hubert WTP lagoon system:
➢ Sample Location#1—24-hour composite from NPDES sampling point.
➢ Sample Location#2— Five individual grab samples taken throughout the day at the
NPDES sampling point.
• Sample Location#3— Five individual grab samples taken throughout the day at the point
where water enters Lagoon 3.
➢ Sample Location#4— Five individual grab samples taken throughout the day at the point
where influent enters Lagoon #1.
Sample Location#5—grab sample of solids from northeast corner of Lagoon 1
➢ Sample Location #6—grab sample of solids from northwest corner of Lagoon 1
➢ Sample Location #7—grab sample of solids from northeast corner of Lagoon 2
➢ Sample Location#8—grab sample of solids from northwest corner of Lagoon 2
Samples collected August 31, 2020, included water samples only:
• Sample Location#1—24-hour composite from NPDES sampling point.
Sample Location#2— Five individual grab samples taken throughout the day at the
NPDES sampling point.
➢ Sample Location#3— Five individual grab samples taken throughout the day at the point
where water enters Lagoon 3.
SHEA Y HUBERT W I P IOXICII Y !DENCIFICATION EVALUATION 6

Y Sample Location #4— Five individual grab samples taken throughout the day at the point
where influent enters Lagoon #1.
All samples were transported on ice and were maintained in refrigerator units until used in
testing. Chain of custody(COC) records are provided with the test data for each sample date in
the following appendices:
➢ Appendix B—Samples collected August 11, 2020
Appendix C—Samples collected August 26, 2020
Appendix D—Samples collected August 31, 2020.
3.2 SAMPLE PREPARATION
Effluent samples from Location#1
An aliquot of the 24-hour composite taken at the NPDES sampling point (Location#1) was
heated to a temperature of 25+1°C. The salinity of the sample was adjusted to 25 ppt using
Crystal Sea salt. This salinity-adjusted sample was subjected to a 24-hour pass/fail bioassay
with Americamysis bahia at the NPDES testingconcentration of 90% effluent.
Y
Grab water
samples from Locations#2, 3, and 4
P
The five grab samples from Location #2 were combined prior to testing. 200 mL of each grab
sample were combined to create one composite sample for Location #2. The composite sample
was heated to 2 +1° an
d C a d adjusted to 25ppt usingCrystal Sea salt. This salinity-adjusted
Y Y J
sample was subjected to a 24-hour serial dilution bioassay with A. bahia at the following
concentrations: 0, 6.25, 12.5, 25, 50, and 90%. The grab samples from Locations#3 and #4
were treated in the same manner as Location #2.
If the combined grab samples from any location resulted in a test failure, each grab from that
location was diluted to 90%and tested individually in a pass/fail bioassay.
SHEALY o HUBERT WTP TOXICITY IDENTIFICATION EVALUATION 7

Solids samples from Locations#5, 6, 7, and 8
Solids were processed according to methods in EPA 503/8-91/001, Evaluation of Dredged
Material Proposed for Ocean Disposal, February 1991. Solids from Sample Location#5 were
thoroughly homogenized then 500 mL was placed in a glass beaker with prepared laboratory
saltwater in a sediment-to-water ratio of 1:4.The samples were allowed to stir on a magnetic
stir plate for 30 minutes. When the viscosity of the solids impeded the stirring mechanism, the
solids were thoroughly stirred by hand every 5 minutes. The mixture was then allowed to settle
for one hour. The supernatant was collected via siphoning and salinity adjusted to 25 ppt, if
needed. Solids from Sample Locations#6-8 were treated individually in the same manner.
All salinity-adjusted supernatants were subjected to 24-hour serial dilution bioassays with A.
bahia at the following concentrations: 0, 6.25, 12.5, 25, 50, and 90%.
3.3 TOXICITY TEST METHODOLOGY
Salt water was prepared using Crystal Sea®Marinemix. This commercial sea salt does not
contain EDTA and is the same sea salt used for Hubert WTP compliance toxicity testing. Salt
water was prepared August 4, 2020 and allowed to aerate one week prior to use in testing.
Testing was conducted in a room that provided isolation and the ability to regulate temperature.
Temperature was monitored and remained between 24-26°C. Lights were set for a 16-hour light
and 8-hour dark photoperiod. Light intensity was checked and confirmed to be between 50-100
foot-candles. Table 2 provides required test conditions according to the acute toxicity testing
procedures in EPA 821-R-02-012 Methods for Measuring the Acute Toxicity of Effluents and
Receiving Waters to Freshwater and Marine Organisms (EPA 2002).
SHEA • HUBERT WTP TOXICITY IDENTIFICATION EVALUATION

Table 2: Required Conditions for Acute Toxicity Tests with Americamysis bahia.
Test ripe: Static non-renewal._tare-renewal.or flow-threu=L
options)
2 Test duration 24,48,or 96 h(available option:
5. Temperature: 20'C=1'C;or 25'C±PC(recommended.)
Test temperatures must not deviate(ie..maximum minus minimum
temperature)by more than 3'C during the test(required)
4 Light quality: Ambient laboratory illumination erecommended)
5 Light intensity: 10 20 µE'm is(50-100 ft-c)
(ambient laboratory levels)(recommended)
6. Photopenod: 16 b light.S h darkness(recommended)
7. Test chamber size: 250 mL (reconunended minimum)
S. Test solution volume: 200 mL(reconmrended minimum)
9 Renewal of test
solutions: After 48 h(required minimum)
10. Age of test organisms: 1-5 days;less than or equal to 24-h range in age(required)
U. No.orgIni-m-per
test chamber: 1 C for 4:is:eat and receiving water tests(required minimum)
12. No.replicate chambers 2 is; effluent test(required minimum)
per concentration: 4 for receiving water test(required minimum)
13. No.organisms per 20 far effluent test(required minimum)
concentration: 40 for receiving water test,(required minimum)
14. Feeding regime: .uremia nauphi are made available while holding prior to the test:
feed 0.2 mL of concentrated suspension of Anemia nauplu_ 24-h
old,daily(approximately 100 naupiii per mysid)(recommended)
For the purposes of reviewing WET test data submitted under NPDES permits.each test condition listed
above is identified as required or recommended(see Subsection 12.2 for more information on test review)
Additional requirements may be provided in individual permits.such as specifying a given test condition
where several options are given in the method.
Acute and chronic toxicity tests performed simultaneously to obtain acute chronic ratios must use the same
temperature. salinity,and dilution water.
elEALY a HUBERT WTP TOXICITY IDENTIFICATION EVALUATION 9

During testing, test chamber temperature was measured with an immersion thermometer in
increments of 0.1°C and calibrated against a NIST-traceable thermometer. pH was measured
using a HACH HQ40d meter, dissolved oxygen (DO) was measured using a YSI-55 meter, and
specific conductance was measured with a YSI PRO30 meter. All calibrations and/or calibration
checks were conducted daily and indicated the meters were working within specifications.
Test organisms consisted of Americamysis bahia at less than 5 days of age. Test organisms
were obtained from Aquatic Indicators of St. Augustine, Florida. They were fed newly hatched
Artemia nauplii two hours prior to use in bioassays. A control chart indicating the health of the
mysids was provided by Aquatic Indicators (Appendix E).
Test methodology for the A. bahia toxicity tests adhered to "Methods for Measuring the Acute
Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms". Fifth Edition
EPA 821-R-02-012. Tests were conducted either as pass/fail tests at a concentration of 90%, or
serial dilution tests at 6.25, 12.5, 25, 50, and 90%. Ten test organisms were placed in each test
vessel containing 200 ml of solution. The composite effluent sample included four (4) test
replicates per treatment to best represent compliance testing conditions. The screening tests for
locations#2-8 consisted of two (2) replicates per treatment.
To initiate the tests,test solutions were prepared using class 'A'graduated cylinders and pipettes.
Solutions were warmed to 25+1°C. The A. bahia were fed freshly hatched Artemia salina nauplii
two (2) hours prior to test initiation. Tests were initiated by randomly placing ten (10) mysids in
each test chamber. The test boards were maintained at 25+1°C and under a 16- hour light / 8-
hour dark cycle. The tests were terminated 24 hours after initiation. Control treatment
acceptance criterion at test termination was to have at least 90% test organism survival.
Dissolved oxygen, pH, salinity, and temperature were recorded for test solutions at test initiation
and termination.
SHEALY HUBERT WTP TOXICITY IDENTIFICATION EVALUA 1101\ 10

3.4 ANALYTICAL PROCEDURES
The effluent sample collected from Location #1 on August 11, 2020, was analyzed for the
following: metals, ions(ion chromatography), and fluoride at Pace Analytical, Columbia,SC. The
metals scan included aluminum, antimony, arsenic, barium, beryllium, boron, cadmium, calcium,
chromium, cobalt, copper, iron, lead, magnesium, manganese, molybdenum, nickel, potassium,
selenium, thallium, tin, titanium, vanadium, and zinc.
Salinity, specific conductance, pH, temperature, and dissolved oxygen for all test solutions were
analyzed at Shealy Consulting, LLC.
Table 3:Test Methods for Analytical Procedures
Analyte Method
Bromide EPA 300.0
Chloride EPA 300.0
Fluoride EPA 300.0
Nitrate-N EPA 300.0
Sulfate EPA 300.0
Metals EPA 200.7
pH SM 4500 H+B-2011
Dissolved oxygen SM 4500 OG-2011
Specific Conductance SM 2510 B-2011
Salinity SM 2520 B-2011
Temperature SM 2550 B-2011
4HEALY o HUBERT WTP TOXICITY IDENTIFICATION EVALUATION 1 1

SECTION 4.0 RESULTS
4.1 TOXICITY TEST RESULTS
Test Results for Samples Collect August 11, 2020
On August 11, 2020, water samples were collected from Locations#1 through#4, and solids
were collected from Locations#5 through#8. All water samples were clear except for sample
'E' from Location #4 which contained a brownish-red particulate matter. None of the water
samples had an odor of sulfur, or any other odor.
Solids collected from Locations#5-7 produced a fairly clear supernatant. The solids from
Location#8 produced a brown supernatant. None of the solids, or the resulting supernatants,
had a sulfurous odor, or any other odor.
Table 4 provides toxicity test results for the August 11, 2020, samples. The toxicity test on
composited effluent from the NPDES compliance test location (Location#1) resulted in a 'pass'.
Tests on combined grab samples from Locations 2 and 3 both resulted in 'pass'. The combined
grab samples from Location#4 (influent) resulted in a 'fail', with an LCso of 67%. Toxicity tests
for all elutriates of solids collected at Locations#5, 6, 7, and 8 resulted in 'pass'.
Table4:Toxicity Test Results for Samples Collected August 11, 2020
p g
Location Test Type LCso % Mortality at Result
90%Concentration
Location#1 (Effluent) Pass/Fail 10% Pass
Location#2—Combined Grabs Serial Dilution >90% 5% Pass
Location#3—Combined Grabs Serial Dilution >90% 0% Pass
Location#4—Combined Grabs Serial Dilution 67% 100% Fail
Location#5— Elutriate Serial Dilution >90% 10% Pass
Location#6— Elutriate Serial Dilution >90% 5% Pass
Location#7— Elutriate Serial Dilution >90% 10% Pass
Location#8—Elutriate Serial Dilution >90% 0% Pass
SHEAY HUBERT WTP TOXICITY IDENTIFICATION EVALUATION 1 2

Since the combined grabs for Location #4 resulted in a test failure, each grab sample was
salinity-adjusted, diluted to 90%, and tested individually in a pass/fail toxicity test. Table 5
provides the results for the individual grab samples collected on August 11, 2020, at Location
#4, which represents the influent into the wastewater pond system.
Table 5:Toxicity Test Results for Individual Grab Samples from Location#4
Collected August 11, 2020
Location and Time % Mortality at Result
90%Concentration
Location#4A(Influent) 100% Fail
Location #4B (Influent) 100% Fail
Location#4C (Influent) 100% Fail
Location#4D (Influent) 5% Pass
Location#4E (Influent) 30% Fail
Test Results for Samples Collect August 26, 2020
All water samples were clear except for sample 'E'from Location#4 which contained a
brownish-red particulate matter. None of the water samples had an odor of sulfur, or any
other odor.
Solids collected from Locations#5-7 produced a fairly clear supernatant. The solids from
Location#8 produced a brown supernatant. None of the solids, or the resulting supernatants,
had a sulfurous odor, or any other odor.
The toxicity test on composited effluent from the NPDES compliance test location (Location#1)
resulted in a 'pass'. The grab samples from Location#4 (influent)were tested individually, and
all but sample#4D resulted in 'pass'. Toxicity tests for all elutriates of solids collected at
Locations#5, 6, 7, and 8 resulted in 'pass'. Table 6 provides a summary of the toxicity test
results for samples collected August 26, 2020.
p g
SHEALY HUBERT WTP TOXICITY IDENTIFICATION EVALUATION 13

Table 6:Toxicity Test Results for Samples Collected August 26, 2020
Location/Collection Time Process and Start % Mortality at Result
Time 90%Concentration
Location#1 Effluent Collected @1100 N/A 0% Pass
Location#4A Influent collected @0659 Resin @ 0640 10% Pass
Location #4B Influent collected @1000 Backwash @ 0924 0% Pass
Location #4C Influent collected @1203 No process started 5% Pass
Location#4D Influent collected @1428 Resin @1333 100% Fail
(slow rinse stage)
Location#4E Influent collected @1610 No process started 0% Pass
Location#5—Elutriate N/A 0% Pass
Location#6— Elutriate N/A 5% Pass
Location #7— Elutriate N/A 0% Pass
Location#8— Elutriate N/A 15% Pass
Test Results for Samples Collect August 31, 2020
The toxicity test on composited effluent from the NPDES compliance test location (Location#1)
resulted in a 'pass'. The grab samples from Location#4 (influent)were tested individually, and
all but samples#4A and#4D resulted in 'pass'. Table 7 provides a summary of the toxicity test
results for samples collected August 31, 2020.
Table 7:Toxicity Test Results for Samples Collected August 31, 2020
Location/Collection Time Process and Start % Mortality at Result
Time 90%Concentration
Location#1 Effluent Collected @0800 N/A 7.5% Pass
Location #4A Influent collected @0822 Resin @ 0700 100% Fail
Location #4B Influent collected @1125 No process started 0% Pass
Location #4C Influent collected @1312 Resin @1236 5% Pass
Location #4D Influent collected @1556 Resin @1451 100% Fail
Location #4E Influent collected @1648 Backwash @1620 5% Pass
G SHEALY a HUBERT WTP TOXICITY IDENTIFICATION EVALUATION 14

4.2 ANALYTICAL RESULTS
The following chemical analyses were conducted on the effluent sample collected August 11,
2020: metal scan, ion chromatography scan, and fluoride. Since this sample passed the Mysid
toxicity test, the analytical results provide baseline data for the comparison of future failing
effluent samples. The results for the IC scan, fluoride analysis, and all detected metals are
provided in Table 8. The analytical report is provided in Appendix E.
Table 8:Analytical Characterization of Effluent Collected August 11, 2020
Analyte Result
Chlorite 3100 mg/L
Sulfate 4.4 mg/L
Barium 0.070 mg/L
Calcium 780 mg/L
Iron 0.20 mg/L
Magnesium 17 mg/L
Manganese 0.31 mg/L
Potassium 15 mg/L
Sodium 950 mg/L
Fluoride <1.0 mg/L
SHEALY o HUBERT WTP TOXICITY IDENTIFICATION EVALUATION 15
dri ,

SECTION 5.0 CONCLUSIONS AND RECOMMENDATIONS
The toxicity tests on final effluent samples collected August 11, 26, and 31, 2020 all resulted in
passing toxicity tests. In fact, none of the final effluent samples indicated any toxicity
whatsoever. Grab water samples collected at entry and exit locations from Lagoon#3 also
passed toxicity. Sediment samples collected at four(4) locations in Lagoons#1 and#2
processed into elutriate tests following USEPA methods also indicated no toxicity whatsoever
which was not expected.
The only samples producing toxicity failures were collected at the influent entry into Lagoon 1
(labelled Location #4 on the sampling map located in the Appendices). Grab samples tested
from this location did indicate that toxicity was entering the lagoon system during the study,
even though toxicity was not observed in the final effluent. After the first set of samples were
collected on August 11, 2020, each time a sample was collected from location it was noted on
the chain of custody which water treatment process was discharging. Not surprisingly, the
toxicity for the influent samples varied significantly appearing to be associated with specific
water treatment process that was occurring during the time of sample collection. For instance,
the sampling conducted August 31, 2020 included two (2) samples collected from location#4
during the "Resin" process discharge into Lagoon #1, one test resulting in 10% mortality and
one test resulting in 100% mortality of the test organisms. Both grab samples collected on
August 26, 2020 at location#4 during the "resin" process discharge resulted in 100% mortality
to the test organisms. For the ten (10) samples collected at location #4 during the study the
only samples that were toxic were the ones collected during the "Resin" process discharge.
None of the samples labelled "backwash" or"no process started"were toxic.
As discussed previously, the toxicity tests on final effluent samples collected during this study
all resulted in passing tests. In addition, the NPDES test conducted for September 2020 also
resulted in a passing test. Since no acutely toxic final effluent samples could be obtained during
SHEALY Al I P �`�It � l li?i r I )` �I 'v

this study and since passing NPDES tests were observed for the second and third quarter of
2020, the TIE was terminated.
It is not known at this time what caused the toxicityat the Hubert WTP prior to June 2020, but
it appears that currently the lagoon system is removing the toxicity entering the system from
the water treatmentprocess. It is also apparent from the testingon sediments samples
pp P
collected during the study that toxicity is not accumulating in the solids nor is it being
generated by the solids, at least not at the time of sample collection. However, it is
recommended that the solids be removed from the lagoon system as much as possible since
high solids accumulation such as what was observed during the July 2020 visit, will reduce
water retention time in the system and therefore treatment time. To ensure the best possible
results for aquatic toxicity testing it is beneficial to have as much treatment time as possible to
remove potentially toxic constituents entering the lagoon system. It is also recommended that
each time the Hubert WTP collects a sample for toxicity that a minimum of two (2) extra gallons
be collected and held in cold storage for TIE testing in the event of a future toxicity failure.
HE o
HUBERT WTP TOXICITY IDENTIFICATION EVALUATION 1 7

SECTION 6: REFERENCES
U.S. Environmental Protection Agency, 1999. Toxicity Reduction Evaluation Guidance for
Municipal Wastewater Treatment Plants. EPA 833-B-99-002. Office of Wastewater
Management, Washington, D.C.
APHA. Standard methods for the Examination of Water and Wastewater.
US Environmental Protection Agency(EPA). 1991. Evaluation of Dredged Material Proposed for
Ocean Disposal. EPA 503-8-91-001. Department of the Army, U.S. Army Corps of
Engineers.
US Environmental Protection Agency (EPA). 2002. Methods for Measuring the Acute Toxicity of
Effluents and Receiving Waters to Freshwater and Marine Organisms. Fifth Edition
EPA 821-R-02-012. Environmental Research Laboratory, Duluth, MN.
HEALY a
HUBERT WTP TOXICITY IDENTIFICATION EVALUATION 18

APPENDIX A
SITE MAP WITH SAMPLE
LOCATIONS

i
.... -.. , .,..
. • - - ,-. ',„.'.4.-0,,,,,',. .:-
• ' .'4.•--.2-IFT.SIIIMIES..44.,N14.T.T.X.-" . ,
... , •,, ,
.....
• HUBERT WTP
,
SAMPLING LOCATIONS
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,.
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2020 Diropn Tq.cl

APPENDIX B
AUGUST 11, 2020
TEST DATA

SHEALY CONSULTING, LLC N2 4 5 3 0
AS H EA L Y,14) CHAIN OF CUSTODY RECORD
- "- CONSUMING.uc 343 W. Columbia Avenue• Batesburg-Leesville. SC 29006
Telephone No. [phone] I
www.shealyconsulting.net Page - of f
. Requested Analysis
Client !' Ltp �� / %%E
Address j`
City State Zip Code -
`` Preservaiee
Project Name l 1.Unpres. 5.Ha.
2.NaOH/7ruk 6.Na.Thin
f 3.H2SO4 7.NaOH
Sampler Name Phone# Bottle Size: G,,1 4.HNO3
Preservative Codes: 1
Jompwm 1 S eal i Lab POSample ID/Description Sampler Start Sampler End Indicate number of containers in the boxes below
{Containers for each sample may be comb,nerd on one fine.)�+r c.an ��D,ate Time Date Time
/ —
i rlC',.. C tt lkerid fir_'
i. Rel squished by c n Date Time 1.,F3ec_,eWed by / Date Time, i
.}.1-vi I JI01 i-t(-l2 tie( , 1 Cr r �' 7
2. R ' ed by - e eL 2. Re ved by mate Time
,1 at Tipp
3.Rel wished by ) Da Time 3. Received ., Dad Time
.S c s,�,-� T�rc �3 ai � . S a5 �.- 12L� , �-- .8/I 073, 5
4.Relinquished by Date Time 4. Recei ed by Date Tme
0 Continuation of Custody on attached sheet 0 Received on ice(Circle) Yes No Ice Pack SC Receipt Terri ' r C
DISTRIBUTION:WHITE-Return to laboratory with Sample(s);CANARY-Field/Client Copy Document Number:SCF-TS-248 Rev.3 Effective Date: 6/1/2017

SHEALY CONSULTING, LLC N9 4 534
SHEA CHAIN OF CUSTODY RECORD
wmNG.uc 343 W. Columbia Avenue• Batesburg-Leesville,SC 29006
Telephone No.[phone] Page 11 of
www.shealyconsulting.net
1
Client
dbl.horf I lit
Il.I.MllII .I
Address —�J�
City State Zip Code . -
x
Project Name 1 Preservaive-- 1.Unpres. 5.HCL
2.NaOH/ZnA 6.Na."Mo.
3.H2SO4 7.NaOH
Sampler Name Phone# Bottle Size: mow, 4.HNO3
Preservative Codes: 1
Sample ID/Description c "N Sampler Start Sampler End
Indicate number of containers in the boxes below.
(Containers for each sample may be combinerd on one line.) c,.,c Date Time Date Time
1 arc_h N
Ct.+ PD£S 7 y 5-,I'1a 07;(¢
SCUP 2 - L SaMple Po :in I DI (sJ
cafnple 2 - 6 L= ..,(-A. ocr em4. N
. .-4S le 2 - ` i.`1�.Lt c T
sa p le 2 - i) G- c-it-2--.) cc as.- 3
_ I i - �,
.54 rn.pk 7 - L w C- t i.a,a- /7!b I 10.1 ..
1.Relinquished by Date Time rued by Date Time 1
2.R . hed b to . Arne 2Beceived by to Time—. c‘j Lif
3.Relinquis by to Time 3. eived by D e Time
S�' r�_LK /-fir ' 1 a� C S 3 �� -- 6�1212z C U 3
4.Relinquished by Date Time 4.R ce. ed by Date Time
0 Continuation of Custody on attached sheet 0 Received on ice(Circle) Yes No Ice Pack SC Receipt Temp` 'OC
DISTRIBUTION:WHITE-Return to laboratory with Sample(s);CANARY-Field/Client Copy Document Number:SCF-TS-248 Rev.3 Effective Date: 6/1/2017

1
SHEALY_. CHAIN OF CSHEALYOUSTODY NSULTING RE, LLC
CORD Flo
�`l'`
_.--. CONSUO NG.uC 343 W. Columbia Avenue • Batesburg-Leesville, SC 29006
Telephone No. [phone]
www.shealyconsulting.net Page of
Requested Analysis
Client
}i LL bor.+ --T--1 t---
Aad-ess -Z)
City State Zip Code ie
G Preservaive
Project Name I.Unpres. S.HCL
2.NaOH/ZnA 6.Na.Thio.
t/, 3.H2SO4 7.NaOH
Sampler Name Phone S Bottle Size: L, i 4.HNO3
Preservative Codes: 1
Sheaiv Lab ID
Sample ID/Description pOS'« Sampler Start Sampler End Indicate number of containers in the boxes below.
Containers for each'sample may be combinerd on one tine.) o.�c Date Time Date Time
�i 3 .ri H '5 wader- Qnfcr ) 1 1.41-2J v7:24.
S��',� E L_,_A.c r-, i 3 l - J 1 -J L, f c:
r S"- ') -C'�-31
Xt,n��E .3-- C CT 'ems- (i-,2-' t f 3�- i
I if ,,R_,,
sarrplf .3- C�- y- l rJ t I
/ J �- I i
—L`
_Sample 3 y (7=,i' ti-A (7' 1 1 J~. I -IcI
7. Relinquished by Date Time R c rued b
Y ( Date Time
2. ed by Date Time 2.gec ved thy Date Time
3.Relin uuiiAhed lay to Time 3. Received b r Date Tim J
4.Relinquished by Date Time 4.Rec'eiu4d by / Date Time
❑ Continuation of Custody on attached sheet 0 Received on ice(Circle) Yes No Ice Pack SC Receipt Temp"? 'C
DISTRIBUTION:WHITE-Return to laboratory with Sample(s):CANARY-Field/Client Copy Document Number:SCF-TS-248 Rev.3 Effective Date: 6/1/2017

SHEALY CONSULTING, LLC N2 4536
SHEA -4 CHAIN OF CUSTODY RECORD
NSUUnNG LLC 343 W. Columbia Avenue• Batesburg-Leesville,SC 29006
Telephone No.[phone]
www.shealyconsulting.net Page of_t_
Client Az j r _J, i r E
Address �l' l
T-J
City State Zip Code r
Preservaive
Project Name I.Unpres. 5.HCL
2.>JaOHi'ZnA 6.Na.Thio.
i/ 3.H2SO4 7.NaOH
Sampler Name Phone Bottle Size: Cx.� 4.HNO3
Preservative Codes: I
Sample ID/Description o�'° Sampler Start Sampler End Indicate number of containers in the boxes below.
(Containers for each sample may be combineerd on one line.) c.,6 Date Time Date Time
SQrr)ple - rG5 ift-Y k.ct7,4- z-rIftrsj g 1�, O7.Z3
LC L 0 c-r, / I _ J 0 l 5
Sc r,,- ie -6 i,.— L'-1 b I h C I -(.,r,
-�
�Sarrt p/E ✓4r-ti-,z� ,s°J i T
tr I
1.Relinquished by D to Time ived by Date Tim
V-(/-�v (7Z /
2. Dished by t o 1 2.deceived ky to Tim
C
3.Relin !shed bX e Time 3. eived b Date , Time
4.Relinquished by Date Time 4. Recejed by Date Time
0 Continuation of Custody on attached sheet 0 Received on ice(Circle) Yes No Ice Pack SC Receipt Tern 4O
DISTRIBUTION:WHITE-Return to laboratory with Sample(s);CANARY-Field/Client Copy Document Number:SCF-TS-248 Rev.3 Effective Date: 6/1/2017

SHEALY CONSULTING, LLC N° 4537
SHEA CHAIN OF CUSTODY RECORD
N$ULUNG.LLC 343 W. Columbia Avenue• Batesburg-Leesville,SC 29006
Telephone No.[phone] Page 1 of I
www.shealyconsulting.net
Client f 'u i +- `
(7 ►7F r d I
Address
City State Zip Code LI
Preservaive
Project Name �� 1.Unpres. 5.HCL
2.NaOH/ZnA 6.Na.Thio.
I 3.H2SO4 7.NaOH
Sampler Name Phone# Bottle Size: L. 4.HNO3
Preservative Codes:
Sample ID/Description cc"'poste Sampler Start Sampler End
or Indicate number of containers in the boxes below.
(Containers for each sample may bye combinerd on one line.) cyan Date Time Date Time
Sarvp(E- .5 — H r= /ern.e r' L 5 o n n I /1J , 1;d3j i l-.2 ( 3 03 J 0 CD
Sam ple ic` (sI J Corve con I LSD t o1 �-l1-'1, t' 3( ) _
1 Tot s i
� p k 7 N t= Corn c r L y -r 07 [s o ;cs 1 Wit` -2' ( s i �
1
.. r � I � .
c/
Sample 8 -- C r-twr ld400n 02 L ; i:cls-7 I r -z o ! 3 22 J i S 3
1.Relinquished by Date Time (vyby e to Tim@
f� • �� cr-�0 t 7 0� _ ��7 — C' i f
2.R .iited by ' afe T' e� 2.R e1 ed by t? Time'=
3.Reljpi�ish by t Time3 3.R ived by Dat Tin 3„
, ,
4.Relinquished by Date Time 4.Received by Date Time
0 Continuation of Custody on attached sheet 0 Received on ice(Circle) Yes No Ice Pack SC Receipt Tem,'4'C
DISTRIBUTION:WHITE-Return to laboratory with Sample(s);CANARY-Field/Client Copy Document Number:SCF-TS-248 Rev.3 Effective Date: 6/1/2017

Shealy C'ointdhug-Lt.(' Page I of I
Document number SCF-TR-322 Replaces Date 10,1 1�I1
Revision nwnbcr 2 Effective Date.0025,1 i
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis bahia
EPA 821-R-02-012(METHOD 2007.0)
CLIENT: K l e le S cr, / fu 13.e-1- Lk--) T
SAMPLE LOCATION: ,)(� m p / " eer»71Qs rto
TEST CONCENTRATION (IWC): 90
Test Start Date/"Time: i 5 tt Test End Date/Time: 8 /13 /aoL I750
Test Organism Age: 3 dal Test Organism Source: A )- Temperature of Organisms at Initiation: 0?4.3
Anemia Lot Number: T 2v-44 S I Salt Water Batch: 080 1-1 a 0 - .a1 Salinity: a 5 960 Incubator#: f}t.t o eX
Date/Time Fed prior to Test Set-up: 67/ia(20;.4? L I S4 5 Date/Time Fed @ 24 hours: IV/t4
Test Conditions(Check if in compliance,describef non-conformity if not):
Test Solution Volume:200 mL .Test Chamber Volume:250 nil. • #Mysids/ Rep: 10 • 16 Hour Light/S Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
( DATE/TIME AT TOX LAB
sr.016 8/i Tao V UG THROUGHOUT
sit 2/2to O S 25
Test Initiation 24 Hour Reading Test Termination
Analyst FLA) T
Date s Ia/ao B I1 30-C.`
Time 1750 1 35 0
Aerated at
Start? How
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-Karber or l'rohit
48 Hour LCSO: > 90 95'!% Confidence Interval: 0^1
! flS -
COMMENTS:P ` S

ShealyConsul LLC 2 of 3
�B. Page
Document number:SCE-TB-322 Replaces Date:I0/l Pt 3
Revision timber 2 Effective Date(16R5/I I
Client Name: f9 _`De rJ T I L Sample ID:"To I b4 Initiation Date: g/0/2Z)
ACUTE SERIAL DILUTION TEST for Mvsidopsis bahia
DAILY MORTALITY and WATER CHEMISTY DATA
%
Treatment Replicate # Dead # Dead Mortality
24 Hours 48 Hours 448-Hours
Control q 0
B p
ae
D p
N..' ,
A a 10 /
90 /. B
0 D tr)
q
B
B
B `\
A
B
WATER CHEMISTRY DATA
Temp. D.O. plI Salinity Temp. D.O. pH Temp. D.O. pt1 Salinity
Treatment (°C) (mg/l.) (SU) (ppt) (DC) (mg/L) (SU) (°C) (mg/L) (SU) (PO)
SM2S50B SM45000G SM4500HB SM2520B SM255OB SM450000 SM4500HB SM2550B SM45000G SM4500HB SM2520B
0{IRS 0 FIRS 0 HRS 0 FIRS 24 HRS 24 FIRS 24 HRS 48 FIRS 48 FIRS 48 FIRS 48 FIRS
i )L. a 8.9 g.a js. 3 j25.a 70 8. 1
go/ ,24.3 9.5 8. 0 .241.4 Jti_o 6, b B. I
5c�
DO Meter N U6E1533 OR FI Meter p120200067017/t32612567033 Thermometer P 'I 1p ( 3 Conductivity Meterd VS I Pn 30
Ism ►oszSz /
Comments: /to J /00 32 3

Shealy(son,tdttng.LLC. Page 3 of 3
l.N.ctumad tuunber SCF•TB-322 Replace Date 10/11/13
Rcwston ntunber 2 Effecnve Date ab'23114
Client Name: at,t_ber7 Loc& on Sample ID:J C I(e`I Initiation Date: lI e2/.ZU
DATA VALIDATION:
LEVEL I: LEVEL II:
1)Slats input correct? Yes No
CONTROL VALIDATION: 2)Does test pass? Yes No
Mortality < 10% ✓ 3) If test failed,has client been notified? I I Yes No
4)Date of notification:
ADDITION VERIFICATION: 5)Is DMR attachment correct? Yes No
Initials of 1'(mortality talc: E �t 6)Is test cover sheet collect? Yes No
Initials of 2nd mortality talc: 7)Is dilution series 0.5 or greater? Yes No
Data input requirements met? 8)If there are any protocol deviations.are they discussed Yes No
in the Case Narrative',
All sample holding times met? "'
Sample information collect(see COC)?� LEVEL II by:
Temperatures in range?
LEVEL I by: E7

islality:Hubert Now
Analyst:EV✓T rat pt.,.Iron., ,at n,venomerlda.a,elate summer, rnt+Nu atN a sn,.ndpaww.a heir Ow'Crna anon'au an'
Test ID:Si m t!1 Coposde
r e,IO e.w.
--- - - to.avarrwewywlwrMeh Iron moadle tntt to Mr 11.61.1N eats m tew'Sweto stems;Lorton ewer
Date:8/12/2020 roe Wooled ernes.tent IV.ton NOtCII.eC in nl.rrlee Wow to,no eel mewls rdtatted
lab ID:Shealy sirseen
Species:Atneacemysls bahia(myned shnmp)
Test Type:Acute Survival Select High or low:
IWC Concentration:90 NDIV/01
Hypothesis Test:NON/01
Mint Estimate Test: Unto,Interpolation
IStaIIetIwDats eons Mean renter, AMe Way CV 101V I
D
ate w the Irahue honed vn 1.412016112 1 0 0.000
N a n m
eese...for enecnm taadntawn NA
NA
tw,emr dntrArwn not mxtd. —... NA
NA
Venencenol rest. NA
prrhwe names Our..of opera NDN01 o01V01 Nate•Pot stahftc.tests.'NS'.ndlules that!hecnncentre:onit
1 / NDrv/o! NDN/ol not,lee,ttwu rn yallerwuhulne tont rot.woe.e' .utnmet
�mwwnrel dr,war need uata.r fro conunaratron.e Natnucaay mereeent renm the cones+
+.+n. NON/01 NON/0! 11OIV/01 NON/01
Tea Acceptability CAusla
ICSO s90 Coterlen TM VeRr Pass/fall TAC Nags
lower 9S%Ca.Odance Mersa NA Control mean rcsponw n 9 1 Pns No Rap
Ilgae/S%Ce.Manee Interval NA NA 1 NA_ Note-TIN east alt.is never transformed for roc comer non
Control CV NA 0000 NA •i
Wool NAI eMtp NA NA NA
ne NA NA
TNT Pan Nola-it only two concern,aeon,owe entered,caloJatet pool estimate rota NOEL,NOAEC teat a Wpontt
CakolaN hole NA are na aPplume.
Table hole NA
Roletiva N(Nett et'WC 0.00
a0:VrO.
#01V/0.
Toxicity Test Results
1.2W
1.000 • .. —t,ewhhe.n,
0.200
0000 - • O tx41i--
0 90
Ceee.ntralcm

Shealy Consulting,U.C. Page I o(l
Document number.SCF•T&321 Replaces Date MI I fill
Revision number 2
F..ffemive Dale 06/25014
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis bal:iu
EPA 821-R-02-012(METHOD 2007.0)
CLIENT: L W L i L i< crn / r I.
SAMPLE LOCATION: JC1-rry)l L 0 c coti&ri #
TEST CONCENTRATION (IWC): I
Test Start Date/Time: `C'(ialo"l.o o 055 5 Test End Date/Time: Si i3/.90 1355
Test Organism Age: 3 L'i 0Test Organism Source: A .1-. Temperature of Organisms at Initiation: ?4.3
Anemia Lot Number:TW 'I 5'I Salt Water Batch:S W e$( 0,p—. j Salinity: a5 96o incubator#: Annex
Date/Time Fed prior to Test Set-up: 8/I 2/a-037-0 I51-UDate/Time Fed Q 24 hours: ._
Test Conditions(Check if in compliance,describe non-conformity if not):
Test Solution Volume:200 mL .Test Chamber Volume:250 mL • k Mysids/Rep: 10• 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
DATE/TIME AT TOX LAB
ROUGHOUT
£ 0mf)Cs,tit 8/11 I,J42 ENTIRE TEST 8'14 d ,39
Test Initiation 24 Hour Reading Test Termination
Analyst Ln - t.„._.0
Date I.a/°Z0°2e 53'13/ -0-
Time 15 5 155
Aerated at
Start? Flow
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-Ka rber or Probit
48 Hour LC50: > 90 95% Confidence Interval: N -
COMMENTS:

Shealy ibmamhang,LI.0 Page 2 of 3
Donm:nt number St"F-Tli-322 Replaces Date:IO/t 1/13
Revision number 2 Effective Date.06%25/1.1
•
Client Name: �:�(����r Lk) Tf At- t Sample ID:SO 1(o 9 Initiation Date: 6/)o?/ao
ACUTE SERIAL DILUTION TEST for Aflsidopsis Bahia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
/ 24 Hours \ 48 Hours a4.4 Hours
Control A O \ 0
B O
A co
(e . c `' B 0 0
A
I Q
,1 r75 B O
p A
,L) \ o
B o
A 0
G B 0
10
\.
�� 0 B 5 1.
A
B
A
B
WATER CHEMISTRY DATA
Temp. U.O. pi( Salinity Temp. D.O. pH Temp. D.O. pH Salinity
Treatment (°C) (mg/L) (SU) (PPt) (°C) (mg/I.) (SU) (°C) (mg/L) (SU) (PPt)
SM255011 SM45000G SM4500HB SM252013 SM25508 SM45000G SM4500HB SM255011 SM45000G SM45001(B SM252011
0 HRS 0(IRS 0 IIRS 0(IRS 24 HRS 24 EIRS 24 HRS 48 HRS 48 HRS 48 FIRS 48 EFRS
Cif aL- 1 9, R 9, a as.a a250 61, v
to. a5 2I.,4 9, 0 g . a 9sa a5 I �.(8 cg•I
I . 5 di!. 9 9. . 1 a 5. i .1s. (t, u.
025 b '-l.J 9. I g. a aso 050 6.8 i
So a'-l.7- 4. 1 ?).I a 4 -7 c24 8 10.7 V . 1
90 au & 9.. 3 E• 1 o2/ 3 Asa (0. 7
DO Meter* 06E:1533 OR 0-F113 pII Meter 0120200067017/132612567033 Thermometer* 310150 3 Conductivity Meter* YS I 1 IV J
I5rY) (032 2/
itv,j- 1003a3
Comments:

Shealy Connmtting,LLC Page 3 of 3
Document number.SCF-TBJ22 Roptaccs Date 'Oil 1'13
Rcvtston mmtber 2 Effechoo Date 06/25,14
JD 1105
Client Name:: 1-.:AI )(;,(.4 LL) 1 r Sample ID: :TO 141 Initiation Date: ^: h2/c9n
DATA VALIDATION:
LEVEL I: LEVEL II:
1)Slats input correct? Yes No
CONTROL VALIDyl ION: 2)Docs test pass? � Yes No
Mortality< 10% 3)If test failed,has client been notified? Yes No
4)Date of notification:
ADDITION VERIFICATION: 5)Is DMR attachment correct? N' [[ Yes No
Initials of 1"mortality care: 6)Is test cover sheet correct? Yes No
Initials of 2'° mortality care: 7)Is dilution series 0.5 or Greater? Yes No
Data input requirements met? ✓ 8)If there are any protocol deviations,are they discussed Yes No
All sample holding times met? ✓
in the Case Narrative?
Sample infonnation correct(see COC)? ✓ LEVEL Il by:
Temperatures in range? ✓
LEVEL I by:

Shealy Cansultin&LLC Page 1 of 3
Document numbs-SCF-TB-322 Replaces Date 10/I 1/I3
Revision number 2 Effective Date 0025/I4
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsisbahlu
EPA 82I-R-02-012(METHOD 2007.0)
/1 ckson j i he•-'�' DTP
CLIENT: (Ai f�J
SAMPLE LOCATION: . t�Mrtr L 4 3
TEST CONCENTRATION (IWC): c
Test Start Date/Time: f,f i d ia,0w 1610 Test End Date/Time: b/i 3/ .) 181 DD
Test Organism Age: 3 dn1 i"f'est Organism Source: aS, Temperature of Organisms at Initiation: a4. 3
Artemia Lot Number:TRU '-I 5) Salt Water Batch:SW 0BO Liao--c25 Salinity: 5b %o Incubator 4:Armeyc
Date/Time Fed prior to Test Set-up: 8 f i a/c20 1545Date/Time Fed Q 24 hours:
Test Conditions(Check if in compliance,describe non-conformity if not):
Test Solution Volume:200 mL -Test Chamber Volume:250 mL . 4 Mysids/Rep: 10. 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
DATE/TIME AT TOX LAB
Camp Csn'ltIiO ' THROUGHOUT NTIRETEST S
11 /t2/a.0 06' - '
Test Initiation 24 Hour Reading Test Termination
Analyst F �7 614D-r
Date 5 II 4a4:::,:.►.z) 8/ 13/ _ —
Time I S IC I f)) 10 l
Aerated at
Start? How
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearm an-Ka rber- or I'ro1)t
48 Hour LC50: > 90 95% Confidence Interval:
COMMENTS: ict m pLt. - 3 = 0200 rr I x_acit. 0.f -J 0 L.>J J ) J o l q.?i
T uI 3j 4 3-017y

Shealy Consulting,LLC
Page 2 of 3
D� un t 4' .t 2 x xn nm�er.<F rn ._ Replaces Date.10/11/13
Item um number 2
EtTective Dates 06/23/14
l
Client Name: //iA ix r 4 cJ - Al/ .3 Sample ID:S O I'i Ll Initiation Date: e / /.9-O
ACUTE SERIAL DILUTION TEST for Mvsidopsis bahia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate it Dead # Dead % Mortality
4 24 Hours 48 Hours a4 ATHours
Control A co 5 %B
6. 5 B o
r) O
B D
ia .5 g
A
c,2 e o O
A o
.ic BCD
90 A0
B 0 C'
A
B
A
B
WATER CHEMISTRY DATA
Temp. D.O. pi Salinity Temp. D.O. pit Temp. D.O. pH Salinity
Treatment (°C) (ing/L) (SIT) (PM) (°C) (mg/L) (St) (°C) (mg/L) (SLY) (PPt)
SM255011 SM450000. SM4500118 SM2S2013 SM2SSOB SM4S000G SM4S001IB SM25501t SM450000 SM450OHB SM2520B
U HRS 0 FIRS 0 HRS 0 HRS 24 HRS 24 HRS 24 FIRS 48 HRS 48 FIRS 48 HRS 48 HRS
etrin4El a5. I 9. $, q a5 a as I -1.0 ' . I 7
6 -25 a5:0 9- 3 g. , as a 07q-14 to.S 8. .;. zz7
is 5 a5..9 9. 3 S. a .25 a DV.3 to. S g. a
a5 a5. 3 9. 3 8. .? asd 024.T 61, g. l
sv c75. 3 9. ,/ ( a117- a43 6. S 8. i
90 d{.3- 9. L/ ° R a4 t 074 a (0. 1- v .
DO Meter ll 06E1533 OR _ 1134 p I Meter 8120200067017/132612567033 Thermometer* 3l015O.1 Conductivity Meter** VS I RE,30
t5m to32 2/
Comments:
Us 3 1CM 323

Shealy i onstdttsg,LLC. Page 3 of 3
Ductunent number:SCF•T13-322 Replacer Dute 10/11/13
Revtven munber 2 Effective Date 00/2914
ful10 /
Client Name: duiv-r-i t�) re/ T/ 2 Sample ID: T6 t 1 'i Initiation Date: c9j i(ICJ
DATA VALIDATION:
LEVEL I: LEVEL II:
1)Stats input correct? Yes No
CONTROL VALIDATION: 2)Does test pass'? 4 Yes No
Mortality<10% ✓ 3)If test failed.has client been notified? e`�[ I Yes No
4)Date of notification: 1
ADDITION VERIFICATION: 5)Is DMR attaclunent correct? Yes No
Initials of la`mortality talc: 1Z 6) Is test cover sheet correct? Yes No
7)Is dilution series 0.5 or greater? Yes No
Initials of 2"'mortality tale:
Data input requirements met? ✓ 8) If there are am protocol deviations.are they discussed Yes No
All sample holding times metes
in the Case Narrative?
Sample information correct(see COC)? "
Temperatures in range? LEVEL II by:
LEVEL I by: F1_77

Shealy consulting.LLC.
Page 1 of 3
Document number SCF-TB-322 Replaces Date.1011 I/11
Remon number 2 Effective Date.0ti/23/14
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis bahia
EPA 821-R-02-012(METHOD 2007.0)
CLIENT: cl S err-) / r`i 1,-) 1 r
4
SAMPLE LOCATION: �f�:r i y7 �
r
TEST CONCENTRATION (IWC): 94_1 °Al
Test Start Date/Time: 6la aoj-c I $ 5 Test End Date/Time: kla.2-c
Test Organism Age: sdair Test Organism Source: i Temperature of Organisms at Initiation: 2`t • ,3
Artemia Lot Number:7,20_y 5 I Salt Water Batch: ,30)O130'i o20— o?5 Salinity: 025 %o Incubauor : I nntY
Date/Time Fed prior to Test Set-up: 5/I a/dam 545 Date/Time Fed @ 24 hours:
Test Conditions(Check if in compliance,describe non-conformity if not): ✓
Test Solution Volume: 200 mL •Test Chamber Volume:250 mL • #Mysids/Rep: 10 . 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
DATE/TIME AT TOX LAB
COrr1os;}t, 9/'fl� THROUGHOUT I_
11 ENTIRE TEST Sj l0120 0q 1 O
Test Initiation 24 Hour Reading Test Termination
Analyst AD7'
Date g It '4 7(7 ) g 3/02.0:AD
Time Ic9075 iooZ5
Aerated at
Start?How
Long?
RESULTS:
CeTIS
Statistical Analysis Used: e/eeale-SM Trimmed Spea nit an-Karber or Probit
48 Hour LCSO: CD • 1 cq 95% Confidence Interval: 10-4 . J a - to-4- I Q
COMMENTS: Jo irtnt•¢_ - .200 m I .Q&ck o ' Sep 13 5) i�, i (a DM 1 7-I
,T al-78) To179
F-ou I C) 9v

Mealy ComaInng,LLC Page 2 of 3
Document number.SCF-TB-722
Rtplacaz Date.1011 I 13
Revispn number.2 E)Techvc Date.Oo,2 b 1.1
Tn Is?5 -
Client Name: A/i. 131r4 w rr/ H `'// Sample ID:Y pj 71 Initiation Date: e //./ (j
ACUTE SERIAL DILUTION TEST for Mpsidopsis bahia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
124 Hours 48 Hours .44Hours
A
Control B 0 0
A
toa5 B © O
A 0
// .?. 5 B 00
A 0
r›? 5 B 0 0
A a
SO B 0
90 A /O
B /0 100 /.
A
B
A
B
WATER CHEMISTRY DATA
Temp. D.O. pH Salinity Temp. D.O. pH Temp. D.O. pH Salinity
Treatment (°C) (mg/L) (SU) (ppt) (°C) (mg/L) (SG) (°C) (mg/1.) (St!) (ppt)
SM2550B SM45000G SM4500H13 SM2520B SM2550B SM45000G SM4500HB SM2550B SM45000G SM4500116 5M25200
1' 0 HRS 0 HRS 0 HRS 0 HRS 24 IBIS 24 HRS 24 HRS 48 FIRS 48 HRS 48 FIRS 48 HRS
61n144 0)S 7 9. a s. a Q 5 a .25 5 (o.(i R. 1
te..15 as.a y. a 13. a5 1 .25`{ 6. S. l
/02.5 n;5, 3 9 a 9. •2 a51 .25 ( IP- 4 S- d
./7-7
a5 -2s a 9. 3 $. d °2y. 9 c.2c..0 (c (c E>
50 a-5 o 9. 3 °• 1 a'/( (Q5 9 (.0-4 S . ,
90 a?f 3 (-)I- 5 t3. d &a/4, a 5 5 (o.(.o o
DO Meter M 06E1533 OR SF 1 pH Meter N120200067017/132612567033 Thermometer M 3/0 I 5 o 3 conductivity Mcter# VS I Prn 30
1Snn103252/
Comments:
it. T too 323
1

Shealy nnw IIii LLC. Cage l of 3
Docwnenl!lumber SCF-TB•322
Reple va(hie I0i11A3
Revisicm numbx 2 1.7hrchve[Ale.0025/14
fC�li5 —
Client Name: 1lf.c.. 4 e3 T Sample ID: To al g Initiation Date: <L2// /DATA VALIDATION:
LEVEL I: LEVEL II:
1)Slats input correct? Yes No
CONTROL VALIDATION: 2)Does test pass? Yes No
Mortality< Io% ✓ 3) If test failed,has client been notified? _ Yes No
4)Date of notification:
ADDITION VERIFICATION: 5) Is DMR attaclunent correct? Yes No
Initials of 1°mortality caic: w1 6)Is test cover sheet correct? Yes No
Initials of 2"`'mortality calc: 7)Is dilution series 0.5 or Greater? Yes No
Data input requirements met? 8)If there are any protocol deviations.are they discussed Yes No
MI sample holding times met? in the Case Narrative?
Sample information correct(see COC)? LEVEL II by:
Temperatures in range?
LEVEL I by: fL,s1

•
Summary Sheet
Fundy Hubert Analyst E9T
Test lD See 4 Comb.nerf Species Amencomye.a Dann in rvemd shmnp)
Dale 6r122020 Test Type API lie Coruna;
Lab ID Shealy MC Conc. 90
Input
Number of Ornanisms Exposed no Counted
Conamwelio s
Replicate 0 0.20 12,5 25 50 90
I 10 10 10 10 10 10
10 10 10 10 10 10
Number of Orgasms Sutvmryl of Reapondaq
ConcenVabons
Replicate 0 6.25 12.5 25 50 90
10 10 t0 10 10 0
10 ie 10 10 10 0
Total Ornsnoms 20 20 20 20 20 20
Total Responn,nd 20 20 20 20 20 0
4 Rospondinq 1000% 1000% 1000% 1000% 1005% 00%
Output
Steinke)Oata Conc Mean(Transformedt Mean Stdav CV Start lest
slaexs ae m.ea on. Nonau wu.aea..n,w l.wo 0 1412016112 I 0 0000
nansy,,,p us vre b, 625 1412016112 I 0 0 000
Mon.cae awm 125 1412016112 I 0 0 000
Va..,....ley. 25 1/12016112 1 0 0 000
SO 1412016112 I 0 0 000
t nw Oa.*et nsen 90 0 0 v
,.....a... .,amr n ire r,eeuu rime.,M.er.Ka,n+ NA
Test Accepatxhty Crasns
Coronae tetl Value PasaiF en Crosier Test Valve PeslaFeo
Control mean .=09 , Pass N4 NA NA
response NA PM6p
NA NA NA NA
Coelho'CV NA I NA MOO 'IA NA NA
TAC Fags NO naps
' Hypothesis test Steam Many-One Rnni Test
Point estimate lest Loom Inlnrpolahan
NOEC LOEC LCSO 95%Confidence Intervals
67 12 67 12 67 12
TOT Calculated t-value Table t-value Relative%Effect at IWC
NA NA NA
Note-For stebstcai tasts.'NS'motets,nest Oa co,.cenreahon n nal staes6c0tly different from Isle control.acre 'C,ndcales that the concentration
$hlalMrcany anernnl from the control
Toxicity Test Results curve type r
1!MI
1 qkf --._..,�__e.,...e�._ ., ... —.......v...
r
A 06ob
r.
0400 •
0 21x1
C OR) . ..,
0 6.25 12 5 25 50 en
Concentration.
NOTICE
The Unite0 States Environmental Protection Agency(EPA).Through cis Office of Wastewater Management.funded and managed the
development of the whole effluent toxicity(WET)Tool described hens This,s a too'that Calculates WET test sndpants for the EPA-
anemved WET test methods and is used by FPA internaiu for analy,mo void WET lest dale Neither the EPA nor env of then
6/132020 PaOo I nl I wet_analrtKarspeaM0not-20190910

:Mealy consulting.I.I.C. rage I of
Document number SCF-TB-322 Replaces Date I Oil 1113
Revision number 2 EITecuve Dale 06/25114
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis bahiu
EPA 821-R-02-012(METHOD 2007.0)
CLIENT: ' )KILOi cksar, / 1tr?i 0 r11
SAMPLE LOCATION: J mp o. `- - iiid, tr rri&wi Saar )Z2A)
TEST CONCENTRATION (IWC): cl(_1
Test Start Date/Time: `;'1 ' I LI-/ '3-20 1315 Test End Date/Time: b /15/aoa•O I 3 50
Test Organism Age: 5 ci @a, -- est Organism Source: rt Temperature of Organisms at Initiation: ,25 I
Artemia Lot Number -4 J ' Salt Water Batch:Sl, JO13 0 4 a0•- o/5 Salinity: c-5 9Go Incubator#: fin Q
ty nn t
Date/Time Fed prior to Test Set-up: S l 1y/ o i I o0 Date/Time Fed @ 24 hours:
Test Conditions(Check if in compliance,describe non-conformity if not):
Test Solution Volume:200 mL •Test Chamber Volume: 250 nmL • #Mysids/Rep: 10 • 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
DATE/TIME AT TOX LAB
—0I —} S -- THROUGHOUT
J t)11 i S I 1 1 ` ZC..1 ENTIRE TEST g I I e2/2 09 10
Test Initiation 24 Hour Reading Test Termination
Analyst f', A )T f�rr
Date 8/14/20:20 g 1 I s j
Time 1 3 15 1 3 5_)D
Aerated at
Start?How _
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-Karber or Probit
48 Hour LC50: 95"A Confidence Interval: -
COMMENTS: 14 N z F't
4 13 z FoLz
LkC L 1
-LI p - Pa4 , 4 lD Pass
ear a i 1 tpo I-4 E ; Fal I

Shealy Comultuig,LLC.
Page 2 of 3
Document number SCF•T8d22 Replaces Date IOil lay
Revision number.
2 Effective Date.06i25/14
Client Name: /4/4 Ile r'1- 1 I t/ Sample 1 v2o oLQ�ID:( fJ Initiation Date: O I
ACUTE SERIAL DILUTION TEST for Mysidopsis bahia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
24 Hours 48 Hours ,44 48 Hours
Control A
B 0
t4 a A I 0
:JOi15 B I 1 CEO
B I 00
►0
4C .Tol11- B IA 'D I0O
41 I. A
DI �� B o 5
Li lot 79 B 4 30 a/.
A
B
A
B
WATER CHEMISTRY DATA
Temp. D.O. pH Salinity Temp. D.O. pH Temp. D.O. pit Salinity
Treatment (°C) (mg/L) (SG) (ppt) (°(') (mg/1.) (SU) (°C) (mg/L) (SU) (ppt)
SM2550B SM45000G SM4500HB ShI2S2011 Sh12550B SM45000G SM4500HB SM2550B SM45000G SM4500HB Sh1252011
/� 1 0 FIRS 0 FIRSI 0
FIRS 0 FIRS 24 FIRS 3 24 FIRS 24
24 ORS 48
48 FIRS 48 FIRS 48 FIRS 481-IRS
W04),01 c25.Lp T 10 O 1 a5.0 c/.1 10.6, Z�. �C
Li 0 a 5. 5 -4. too B aQ.0 o?/ t 6. 3 7
7 7-
I-a .25.5 1- 1-I : 3- aUl.o wq 1 _3 .-e,,t .z,
y c Q 5 I 7.1 -fig LI. ) a4.3 tag 1 -
Li D -2 5 7 .1 4 8. o a41- aq to- 3 S. 3
4 E, aLi, 9 -7 5 3. ai-I.$ _ .2,.0_ 6.5 g ) _
DO Meter M 06E1533 OR 05FI I3 p11 Meter M120200067017/132612567033 Thermometer N d WI 5 0 3 Conductivity Meter# 4 S t Pa 313
15rnt0325L7
Comments: up 3 ic.00 323

'ALMly(�'nudung,LLC Page 3 o(3
DOeun enl number.SCF-TB-322 keplunxs Date.10,11,13
Kevnion umbel 2
ECretuvt Date O6/1S/14
Client Name: NL,.h'(I I ( � Sample ID: Initiation Date: 8 J I`{`ow' ;)-D
DATA VALIDATION:
LEVEL I: LEVEL D:
1)Stats input correct'? Yes No
CONTROL VALIDATION: 2)Does test pass? Yes No
Mortality < lO% w/ 3) If test failed,has client been notified? Yes No
4)Date of notification:
ADDITION VERIFICATION: 5)Is DMR attachment correct? Yes , No
Initials of 1s`mortality calc: Ca..") 6)Is test cover sheet correct? Yes No
Initials of 2"`'mortality calc: 7)Is dilution series 0.5 or treater? Yes No
Data input requirements met? .� x) If there are any protocol deviations,are they discussed Yes No
in the Case Narrative?
7
All sample holding times met?
�
Sample information correct(see COC)? '' LEVEL 11 by:
Temperatures in range?
LEVEL I by: Et.*iT

Samplc. L1 D
Pam
facility:Hubert Nes.
Analyst:EWT owaeww ton.aemtan of 4t..Ms soon...MenneA..net,a+Jt":' tM'Ten natelbpn-
TettIDi Site40 OM1w below
tot*w„tawa Wwsnavn fron.wiAle tem tot.%atntst tee.cot on t.-t*to Wenn.'Moan below-
Date:8/14/2020 ow*Noun.sw...teen......n.nO.51 war,in In.NpC/Iqf n t.l,at!Way to ns*neep.rtt aaMaavN
tab ID,Shealy stint..
Species:Amencamysts bahia(rtrysid shrimpl
Test Type:Acute Survival Select Nigh or Low
MC Concentration:90
Hypothesis Test:T-Test with ttonferroni Adjustment
Point Estlm.te Test: PREF!
IStatistieN Data Cont. Mean TrentIonerel ntaa. SIdev CV T-teal
212 1 0
.•,.441... r 930942 0.95 D.11573743i�
•de,ned r..e„wa1 .ew,. aA ,.na
NA
NA
i)ra a*Neengeneov.va,w,ce NA
Orr awe..eo,.ai mnree,d reyeMr NOAEC 90 Nate sor ttanstu,testa'NS`ndreales that the concentration n
--- not Nat,tecany ANrent Nom tee come...A.le'y nnocatet teat
1.OAEC 790 tea concentration Lt statittvanv Afferent Iron the rontro,
Test Acceptability Criteria
1CSO M(EI COlette t Test Value Pass/Fell TAC flags
bonertf%C..A4nw Wendel MEft cannotneenroseer,v aa0.9 1 Pass No nags
Upper 95%Confidence YeertM1 MEfI NA 1 NA Note -e.!ev.a.,.e•s never trsMm twea la TAC comp....tone
C*e.I CV NA 0.003 NA
Mod NA] IMsO NA NA NA
NA NA NA
1ST NA --_- Not. It one,Iwo tonoentratrons were entered,"nowt*Paint estimate 4N NW,NOMC test.n,oants
C04eMN 1-eMe NA are rot IOW,ralw
Table t,Mle. NA
Aeletlw%Wen at 11VC
Curve Type 1
Toxicity Test Results
I.20D
1.000
0110
10.5D0
1
0.403
1
0.000 _.-__— 0ODD.
0 10
Cancwxralkts

samfk LE
Fcz1
Farah},Hubert now
Analyst:EWT p.m
and..mIn*. tv.rne ntdae non
...,an .m ode. b one..tn1 on On'Grr�ul...rm
test ID:Sit.a E
a.daY oe
Date:a/14/2020 n.net,n...egorrw.,..nnn.neetOldslpn.r Nano.CA C not ektsbe*n Pa'Lot Somenrmoans ace tone
r.r.eeM d.tarwprw..uww.uil.n.40.r 1 oI.WA Clrue(w rM lobs Yore in men,soon,ant a..M
Lab ID:Shealy ,rants,
Species:Americamysls bahia(mysd shrimp)
Test Type:Acute Surswal Select High Of low:
IWC Concentration:90
Hypothesis Test,T-Tea with Bonferronl Adplstwent
Point Estimate Test: NEE!
ISt.bdirel 0.la Cent n1..n ltrenn.m.d) I M can --_ Shen CV T too
111/P0 .I
t 1.412016112-. 1 0 l',1".04111111111M
art 6fted am n.l(ranter"... a02i'79
I 90 _ o9966t2171 0. nl 115
V da..nq r
I...na.,a t. aa x.dn, NA
NA
Ua•rrnorm"O..tIlbIas NA
NA
NA
Note.for statdbul[Ws."NS'wed,tat that the cnncentatwn,s
not stadstkally 6Nerent from the control.while"V"neeatat that
LOAEC itla con werelan is tUtntlt y Afferent hem ins contra
Test AaepteblUt)Criteria
1CSO 30111 Criterion Tee VMr Pass/Fail TAC Rag,
lower 9S14 Confide..Mendel allEfl Centro,mean resew.*
t data No fiats
Upper es%coeed.nn Interval MET, NA t NA None•The tad se".h never transformed NO SAC component
(tonne 1V NA 0000 NA
MSpI NAI eMSU NA NA NA
NA NA NA
TST NA N.e. If emit two toNentr.bnns ere entered...kWeted peon estimate and NOW.NOAEC teat endprm,
Calculated S.W. NA arc non wohe Me
Table moles NA
beletlee IS Meet et IWC n.lent•nr 1 w*toned In Ion.lest he.Wan id...nNN to nod n.nnw*wen,..
eeterml•the'mien,'rat r.WAtYh et IAa tot A..ee we USUCA'.Method Gunton.and
Rwemrn.Wtbna leo wl.ele Mk*[dainty(WA)Testmt 1401 fe Owl Ill.)ler pittance in
.I roily ell..content..11on..tprne.artrn
undefined
Toxicity Test Results
1200 -
LOCO
—r.e.thMre,
o4m
I
.�a6DA I ------
I
0.4(3
00:01 — .. . . D 000
0 90
——- - COKMI.Mbet

Shealy C on.utting.L.LC Page I of 3
Utaument number SCE-rH3-322 Replace Date I Oil I l l s
Revision number 2 Flrenwe Datc o425, 4
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysldopsis huiria
EPA 82I-R-02-012(METHOD 2007.0)
CLIENT: C t K I'D C /c S / KiLL/ t r' LJ 'Tip
SAMPLE LOCATION: Sam p Lc 1,f 5 / E/t.ci-r r-a k
TEST CONCENTRATION (IWC): �1 0 „Ao
Test Start Date/Time: Si „1/.1-c302.p 1845 Test End Date/Time: 8l1 31a.o. zu 1 SLI5
Test Organism Age: &lain Test Organism Source: A Z Temperature of Organisms at Initiation: i-. 3
Anemia Lot Number: Two-LI 51 Salt Water Batch:3t j 0130420- 05 Salinity: ia5 %o Incubator#: r,ex
Date/Time Fed prior to Test Set-up: e i 12/2.Oap (5'45 Date/Time Fed tea 24 hours:
Test Conditions(Check if in compliance,describe non-conformity if not):
Test Solution Volume:200 mL • Test Chamber Volume:250 mL• #Mysids/Rep: 10• 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
�p DATE/rI\IE ATTOX LAB
8/i l''o 1 303 ENTIRE TEST S/I�I 1 0930
Test Initiation 24 Hour Reading Test Termination
Analyst E_t..t9 T ur)
Date 8/1.2/ 2 o ey2.O 2 j 13/e0,1-10
Time
845 I y S
Aerated at
Start? How
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-Karbcr or l'rohit
48 Hour LC50: > 9 0 95% Confidence Interval: sit -
COMMENTS:

Shealy t'onsulung,LLC. Page 2 of 3
Doctmtent number SCF-TB-322 Replaces Date IOil 1�1?
Revision number 2 °�
F.ITechve Date Otv2Si Li
Client Name: fr/V-h.ei4 W Tr /E/ &sSampIc 1D:.:5°It�f' Initiation Date: ('-i/%.2/
ACUTE SERIAL DILUTION TEST for Mvsidopsis bahia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
24 Hours 48 Hours aq -sours
Control A
B Q
(o-a5 A I
B \50
B
A
B
WATER CHEMISTRY DATA
Temp. D.O. pH Salinity Temp. D.O. 01 Temp. D.O. pH Salinity
Treatment (°C) (mg/L) (SU) (PPt) (°C) (mg/L) (SU) (°C) (mg/L) (SU) (PM)
SM2550B SM45000G SM4500HB SM2520B SM2550B SM SM45000G 45001113 SM2550B SM B SM45000G 4SOOITB SM2520
I0 FIRS 0 HI(S 0 HRS 0 IIRS 24 HRS 24 IIRS 24 HRS 48 HRS 48 HRS 48'IRS 48 HRS
eeniVC44 5. (0 9. a '3. 2 Asa '2 6 4-) 1p--1, $ I N.
0.25 c25. 1 9- a g- a 05 I a57 I -I- g I
� as � —
la-5 0 9• a S. .250 as-1 (o.Le g I
II
a5 aq 1 9. 1 S v a49 9413 (0 6 I
so e2 Li 5 g .L1 '7 7 '/b 0N. 7 (o-() •..9
90 ,v./ 9 6- 8 7.y a' / 9y-1 (9.(2 --i•.
nc
DO\letcr# 06E1533 OR . '1134 pH Meter 8120200067017/132612567033 Thermometer,/ •S IV•)O 3 Conductivity Meter,/ YS I AO 30
1Sni 103252/
Comments: I(e T 10&3 23

Shealy Catechism LLC him 3 of 3
Document number SCF-TB-322 Replaces Date:10/I 1/13
Revision timber 2 Effective Date 06/25/1,1
Client Name:41.o,j.UP'I 14-5 rP ,5 adv;ASample ID:J O I/30 Initiation Date: l3 f La/'a,C3
DATA VALIDATION:
LEVEL I: LEVEL II:
I)Stats input correct? Yes No
CONTROL VALIDATION: 2)Does test pass? Yes No
Mortality<10% 3)If test failed,has client been notified? Yes No
4)Date of notification:
ADDITION VERIFICATION: 5)Is DMR attachment correct? Yes No
Initials of I't mortality talc: F,.,.Y7 6)Is test cover sheet correct? Yes No
Initials of 2'mortality calc: 7)Is dilution series 0.5 or treater? Yes No
8)If there are any protocol deviations,are they discussed Yes
Data input requirements met? ✓ in the Case Narrative? No
All sample holding times metes
Sample information correct(see COC)? v
Temperatures in range? LEVEL Q by:
LEVEL I by: T

Shealy Congolting.LLC I'age 1 GI i
Document number SCF-TB-22 Replaces Date 10/1I/I3
Revision number 2 Effective Date 06/25/I4
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis ba/ila
EPA 821-R-02-012(METHOD 2007,0)
CLIENT: L J K C kern ¢�t< `1cr 1.3 ri)
SAMPLE LOCATION: J m p? LoCc i 07--i f4 - e/u' ►'�,��
TEST CONCENTRATION (IWC): Q 0
Test Start Date/Time: S// c{/o?() ./r) 19C0 Test End Date/Time: ��'/l3(fi0 I900
Test Organism Age: 33 Test Organism Source: A.I. Temperature of Organisms at Initiation: c2 / 3
Artemia Lot Number:Tzo-`I5.1 Salt Water Batch:ju)OiaD4;24 -a?5 Salinity: =25 %. Incubator#:/inn%
Date/Time Fed prior to Test Set-up: B I I a f c 15455 Date/Time Fed a 24 hours: !V
Test Conditions(Check if in compliance,describe non-conformity if not):
Test Solution Volume: 200 mL •Test Chamber Volume:250 mL • #Mysids/Rep: 10• 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
DATEJfIME AT TOX LAB
TO 18 I 8 020 (310 EN ORE TEsruT 9/1./a 0930
Test Initiation 24 Hour Reading Test Termination
Analyst aT 8L,L3 T
Date 6 f 8/ 13/aoatt
Time , 1900 / 90
Aerated at
Start? How
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-Karber or Probit
48 Hour LC50: > 90 / 95"in Confidence Interval: N IV-
COMMENTS:

Shealy Consulting,LLC'. Page 2 of 3
Doc anent nunber.SCF.TB-322 Replaces Date 10/11/13
Revision number 2 Effective Date 06/25/14
Client Name:du�)..ir� LC re-�1,6 f[,.,_`-fit a..tSample ID:1-I.P Initiation Date: `v/10/ `
ACUTE SERIAL DILUTION TEST for /Ylpsidopsis bahia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
24 Hours 48 Hours .2448'Hours
Control A
B CD 0
A 0
Co. a5 B C.)
A 0
/. 5. B 0 O
A..25 B 0� 0
A
SO B 0 0
,
B I 5 %
A
B
A
WATER CHEMISTRY DATA
Temp. D.O. pH Salinity Temp. D.O. pH Temp. D.O. pH Salinity
Treatment (°C) (mg/L) (SU) (ppt) (°C) (mg/I.1 (Sir) (°C) (mall.) (SU) (pptl
/ SM2550B SM45000G SM4500118 SM2520B SM2550B SM45000G 5M45001113 SM2550B SM45000G S614500116 SM2520B
0 FIRS 0 FIRS 0 FIRS 0 FIRS 24 FIRS 24 FD2S 24 FIRS 48 FIRS 48!IRS 48 FIRS _ 48 FIRS
Corr1101 ,)5 A 9- a g- 2 a 5-a ';251 Cn_1 iii. A
z 7. 3
6.e25 a4/3 9, a S. a a'5 ( .0 43Q
F..,r t•, t Lilo
i.2.5 021.4' 9 3 e 5 a 024I (0.1 8 . d
a 5 62 I 9 g. 3 Q 5 ol t32 i q L9.6 g
so .2'1. 1 o y E. c25a 2y 3 69 0 o . I
90 .2 y I <8 q 8 a c25 I2 (0.4' 8 -l
DO Meter S 06 3 a K '5 1134 pH Meter H120200067017/132612567033 Thermometer S 3/0 J 0_ Conductivity Meters V6I m 30
15 m ro3252/
Comments: ►t.nov 323

Slwaly Consulong.LLC Page)of 3
Document rumba:SCF-TB-322 Replaces Date 10,t 113
Revtsuan nwnber 2 EITecUve Date Don V l
//
Client Name:/%t.i.F tv� IJ n' �� �r I(1 f ..Sample II): To 18) Initiation Date: 6/1c2/;2-0
DATA VALIDATION:
LEVEL I: LEVEL II:
I)Stats input correct? Yes No
CONTROL VALIDATION: 2)Does test pass? Yes No
Mortality< 10% # 3)If test failed,has client been notified? Vb Yes No
4)Date of notification:
ADDITION VERIFICATION: 5)Is DMR attachment correct? Yes No
Initials of 14'mortality calc: 601 6) Is test cover sheet correct? Yes No
7) Is dilution series 0.5 or greater? Yes No
Initials of 2'3 mortality talc:
Data input requirements met? Sl If there are any protocol deviations,are they discussed Yes No
in the Case Narrative?
All sample holding times met? 1
Sample information correct(see COC)?_-' LEVEL II by:
Temperatures in range?
LEVEL I by:

Shedv Cmuulnng,LLC paxe I of 3
Doeuniem member.SCF-TB-322 I.
Ren.um rwwz: 2 Effective
Date 2iiI 3
Dale U0�_ I1
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis bahia
EPA 821-R-02-012(METHOD 2007.0)
CLIENT: (�1 i c L S / Mu 41,2:f Cam' F ('
SAMPLE LOCATION: , S [, m()Le Lto C cA_'r1`b*'�
TEST CONCENTRATION (IWC): 9 0 %
Test Start Date/Time: e! 1240.20 1'1/OTest End Date/Time: 3/i 3 J)O 2O /91(
Test Organism Age: .3 dwrest Organism Source: a__, Temperature of Organisms at Initiation: d2'- . 3
Anemia Lot Nwnber. T 2cw-'45( Salt Water Batch:,S I nJ 09,04 o2SSalinity: a`Jj 96. Incubator#: &
A1�ne/G fI t,420
Date/Time Fed prior to Test Set-up: g l I a/220.>1 U I S 45 Date/Time Fed Q 24 hours: n11 rT
Test Conditions(Check if in compliance,describe non-conformity if not):
Test Solution Volume: 2(X)mL •Test Chamber Volume: 250 mL . # Mysids/Rep: ltt . 16 Hour Light/8 Flour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
n DATE/TIME AT TM LAB
J 01 S c b Jlll)o 1313 TENTIRE EST 8/12/.2,3 05 30
Test Initiation 24 Hour Reading Test Termination
Analyst F.A-)— T
Date f Ia/e2o` w 6/13/(Jir).:2D — --
Time 1 9 10 / 9/0 _
Aerated at
Start?How
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Snearntan-Farber or I'rohit
NIA
48 Hour LC50: > 9QJ 95% Confidence Interval: -
COMMENTS:

Shealy Consulting,LLC Page 2 of 3
Document number SCF-TB-322 Replaces Date !RI 1,13
Revision number.2
F.Recnve Date Qo123r1 t
Client Name:/-!t&h.e(I turf' *7 ekt•fi,R kSample ID:3-015; Initiation Date: 6 I i.lo9-D
ACUTE SERIAL DILUTION TEST for MYlvsidopsis bairia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
24 Hours 48 Hours e2448'Hours
Control A2
B 0
A le .;15 B © 0
(.7)
A
I•,7 5 B 0
A
025 B d
A 0
.Cc_: B a I0
A a
90 B IO '/a
A
B
A
B
WATER CHEMISTRY DATA
Temp. D.O. pit Salinity Temp. D.O. Or Temp. D.O. pll Salinity
Treatment (°C) (mg/L) (SU) (PPU (°C) (mg/L) (SU) 1°C) (mg/L) (SU) (ppt)
/ SM@55013 SM450000 SM4500HB SM2520a 5M2550B SM15000G SM45001111 SM25501) SM.15000G SMJ500HB SM2520B
0 HRS 0 FIRS 0 HRS 0[IRS 24 FIRS 24 FIRS 24 HRS 48 tIRS 48 FIRS 48 FIRS 49 FIRS
atnhcl 9, a 8 a.. 05a ass GO; g .I
L .15- 014 a 9 a S . a as l d4.g c.P.-1. ° . 1
a. 5. 6?93 y. / 8 . 1 as. I a?,/g to.LP g. i \
as aLi3 9, o go 6250 a5,7 rr.to f3 - 1
so - °. 3 7.T a u } c9 5 .3 Io. le 'a o
90 (24 ;2 6 9 74 ay I 25 4 lP S ` o \\:.\\,
DO Meter N 06E1533 OR Usr t 13 pH Meter 9120200067017/132612567033 Thermometer N 3 IO5ii3 Conductivity Meterl /5 7 Pro 3
t5rn1032.51
14 7" la,' a z. 3
Comments:

Shealy ConsdWhe.LW Page 3 of 3
Document number.SCF-TBJ22 Replaces Dope:IWI 1/13
ReV131011 iaunbet 2
Effective Darr 06R5/I4
Client Name: /L.rd IA) T f
/-{u 'lu'h,rfaSample ID:-To Initiation Date: S/i(lam'
DATA VALIDATION:
LEVEL I: LEVEL II:
1)Slats input correct? Yes No
CONTROL VALIDATION: 2)Does test pass? Yes No
Mortality < 10% # 3)If test failed,has client been notified? Yes No
4)Date of notification:
ADDITION VERIFICATION: 5)Is DMR attachment correct? Yes No
Initials of 1"mortality calc: fd 6)Is test cover sheet correct? Yes No
7)Is dilution series 0.5 or greater? Yes No
Initials of 2'"'mortality calc:
8)If there are any protocol deviations.are they discussed Yes No
Data input requirements met': —/—
All sample holding times met? ✓
in the Case Narrative?
Sample information correct(see COC)?
Temperatures in range? LEVEL II by:
LEVEL I by: 7

Shealy Consulting.LW Page I oil
Document number SCF-TI3-322 Replace.Date 10,11 r13
Revenon number.2 Effective Date 06,25,I
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis bahia
EPA 821-R-02-012(METHOD 2007.0)
- - I
/
CLIENT: G&J k LO , C .�r;� 1..I/�.��e rt SAMPLE LOCATION: jr ,p �Le Ld C t_4-7 ) g /�
TEST CONCENTRATION (IWC): 90
Test Start Date/Time: S4/1 /01,G,zc IUD Test End Date/Time: 8/1 3/c2U 2L) /9.X
Test Organism Age: 3 dal)Test Organism Source: A -t-• Temperature of Organisms at Initiation: c 4 3
Anemia Lot Number.iT 2 -''15I Salt Water Batch:jW O o O4.2D •- 25 Salinity: •25 %. Incubator# 4 neX.
Date/Time Fed prior to Test Set-up: 8/I.2/e2Q 1545 Date/Time Fed @ 24 hours:
Test Conditions(Check if in compliance,describe non-conformity if not):
Test Solution Volume: 2(X)inL .Test Chamber Volume: 250 mL . #Mysids/Rep: 10 . 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
DATE/TIME AT TO\LAB
HOU tl
a-0 l 83 gill 1.7. 13�1a ETHROUGNTIRE TESTT $ I.2/20 0 3 O
Test Initiation 24 Hour Reading Test Termination
Analyst et A DT , i \�
Date g/La/620020 8II3/2o.2.0
Time 19020 19 a o
Aerated at
Start?Hoy*,
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-Farber or Probit
J V�
48 Hour LC50: > 90 95 /3 Confidence Interval: N -
COMMENTS:

Shealy Canstdling,LLC. Page 2 013
Doc*unent number:SCF-TB-322 Replaces Date 10/11/13
Ronson nmtber 2 Effective Date 06/25/11
Client Name:f lu 101 4 W Tf'a 9 c L h,c÷A,Sample ID:TOI 3 Initiation Date: //;2/t0
ACUTE SERIAL DILUTION TEST for Alpsidonsis!whin
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
st 24 Hours 48 Hours .24 {ours
Control A
B 0
A 0
Co. 25 B a 0
A CD
1 a. 5 B 0
A
2) ' \
25 B 1 5'/
A
„CO B 0 0
A 6
90 B 0 0
A 1
B
A
B
WATER CHEMISTRY DATA
Temp. D.O. pH Salinity Temp. D.O. p1l Temp. D.O. p11 Salinity
Treatment (°C) (mg/L) (SU) (Mt) (°C) (mg/L) (SU) (°C) (mg/L) (SU) (PPt)
i SM255011 SM450000 SM4500118 SM25208 SM2S5011 SM45000G SM45001113 SM2SSOB SM4S000G n14500118 5M252013
0 FIRS 0 IIRS 0 HRS 0 FIRS 24 FIRS 24 FIRS 24 FIRS 48 IIRS 48 HRS 48 FIRS 48 FIRS
Cant l 0214 (a 9- a 8 a as-a s 5.3 6. 0 2..;.;
cs. a5 a4.4 9. a (t3 a a5.o .24 5 6. 9 o. I
/a, s iq.2 q 1 o 1 a5.a ,?4: 3 ( B g 1
as. .243 0.(0 -79 a5c� .2y 1 6. 8 v
so .24(o - .q 7,6 a 4-S , L/. - (o. S S 0
9D .24 5 6 -Qi 7. 5 a2 i-q a 4.to 67, a .3 .9
DO Meter M 06F.1533 OR SF1134 pH Meter M120200067017/132612567033 Thermometer M ')1050,o Conductivity Meted/ yS1 P1t3o
ISM 1032 2./
I
Comments: LS /00 3 2-3

Shealy t:onsultatg,LLC' page 3 of 3
Doettnrm munber SC'F-I R-1t' Replaces Date 10111/13
Revi tt tuunhet Effective Date 06/25/I4
Client Name: t lit he t./ �/t�fti .(Cet- Sample ID: I'Li_3 Initiation Date: bt // ./,3-0
DATA VALIDATION:
LEVEL I: LEVEL II:
1)Stats input connect? Yes No
CONTROL VALIDATION: 2)Does test sass? Yes No
Mortality < 10% t/ 3)If test failed,has client been notified? Yes No
4)Date of notification:
ADDITION VERIFICATION: 5)Is DMR attachment correct? Yes No
Initials of I''mortality calc: R��-. 6)Is test cover sheet correct? Yes No
Initials of 2n'mortality calc: `�"'1 7}Is dilution series 0.5 or greater? Yes No
Data input requirements met? 4/ 8)If them are any protocol deviations.are they discussed Yes No
in tlx:Case Narrative'?
All sample holding times met? ✓
Sample information correct(see COC)?' LEVEL II by:
Temperatures in range?
LEVEL I by: FIT

APPENDIX C
AUGUST 26, 2020
TEST DATA

•
SHEALY CONSULTING, LLC N9 4543
SHEA CHAIN OF CUSTODY RECORD
uiTING.ii.c 343 W.Columbia Avenue•Batesburg-Leesville, SC 29006
Telephone No.[phone]
www.sheaiyconsulting.net Page_I of
Client /
Address
City State Zip Code Li
pr servaive
Project Name r-^-' /�' I.Unprcs. 5.HCL
I 1 L / Tax ;c t • 2.NaOH/ZnA 6.Na.7hio.
I 3.H2SO4 7.NaOH
Sampler Name Phone# Bottle Size: Q aj 4.HNO3
Preservative Codes: J 1
Sample ID i Description c°"'P"'° Sampler Start Sampler End
Containers for each sample maybe combmerd on one line.) Of Indicate number of containers in the boxes below.
P ceao Date Time Date Time
C "S---1 CM.3 c) Y-*2 G--4-,-. i t D d
---*P`r it,l ni- (1....)c.s.;,21-i c-7-7 I) j----6 I 84-i
1.Relinquished b ( Date Time L �d bye ) Date Time
q y-.I�✓�CS /-��- r� 2 6-2O /(01� 1� \_7�c`J JC
2. \ fished by (7l -- ate 1'` Tske,,0 j 2.Received by .� ;
�L T 1 c l` to Time
3.Relinq is ed by
5 Date • Time 3.ge ceived by . , ^ n to Time 11 :�1-1 $1a�/,� 09OD ��� h�fLut J "
e- �a,712n, F)9DO
4.Relinquished by Date Time 4.R :Zed by Date Time
❑ Continuation of Custody on attached sheet 0 Received on ice(Circle) Yes No Ice Pack SC Receipt Tempt�iC
DISTRIBUTION:WHITE-Return to laboratory with Sample(s);CANARY-Field/Client Copy Document Number:SCF-TS-248 Rev.3 Effective Date: 6/1/2017

SHEALY CONSULTING, LLC N2 4 54 1
SHEA CHAIN OF CUSTODY RECORD
N$UOING,llC 343 W.Columbia Avenue• Batesburg-Leesville,SC 29006
Telephone No.[phone] r
www.shealyconsulting.net Page 3 of l
ClienAL her / LJ TP
Address
I0
City State Zip Code -.`�
41 Preservaive
_ I.Unpres. 5.HCL
Project Name r 2.NaOH/ZnA 6.Na.Thin.
T"1 ` o ic C I 3.H2SO4 7.NaOH
Sampler Name Phone# Bottle Size: �� 4.HNO3
9aa
Preservative Codes: +
Sample ID/Description C"P°S Sampler Start Sampler End
or Indicate number of containers in the boxes below.
(Containers for each sample may be combinerd on one line.) crab Date Time Date Time
�n ue r- 004° !
oi 85
In-/ /t&en1 4C (.. �..2c. (z03 No f4a4,e5S 51-444
I n"PI Ae in ti- LI- D (z- y-,2 t y1 k ee5 ash Esc
: 1)i taS
InP( tAert LI a G— -,2c.. tbj.V Nv p/1cess s, / ( ID
1.Relinquished by Date Time 1 ved Date Time
—I/4V. L. , , 2 g)r(o .2 I �7 . �, .— . -ib j L 17
2.Bel&juishedy by' _ C Dat ll C T�e�, 2. R ceived-by y \ Dete, Time „
3.Relinquished by Date Ti 3.�ewe+ by ^ ,/ Dat Time
C S (.. .S t:C« _( +- sta�/2tz 010.8 C-> �� C ;..JI�c?-�— s j.)''2�: C q0o
4.Relinquished by Date Time 4.IR cellved by Date Time
❑ Continuation of Custody on attached sheet 0 Received on ice(Circle) Yes No Ice Pack SC Receipt Temp/r f"C
DISTRIBUTION:WHITE-Return to laboratory with Sample(s);CANARY-Field/Client Copy Document Number:SCF-TS-248 Rev.3 Effective Date: 6/1/2017

SHEALY CONSULTING, LLC N2 4545
SHEA CHAIN OF CUSTODY RECORD
SUITING.LLC 343 W.Columbia Avenue• Batesburg-Leesville, SC 29006
Telephone No.[phone] Page of
www.shealyconsulting.net —
Client
lV 1.-C 6erv4 T"!L'
Address
City State Zip Code t_ki
\--
Preservaive
Project Name P
I.Un res. ;.HCL
1✓ - Q 1/i run 2.NaOH/ZnA 6.Na.Thio.
3.H2504 '.NaO11
Sampler Name Phone I Bottle Size: t 1' -i HNO3
Preservative Codes: 1
cc'""'''Sample ID/Description cc'""''' Sampler Start Sampler End
Indicate number of containers in the boxes below.
(Containers for each sample may be combinerd on one hne.) Gr,t Date Time Date Time
Loco 5 E 190
-z(0 it30
i-o LcA.-`k ori-1 I 0 I Ci
1.Relinquished by i Date Time ed by Date Time
2.R T _ced by �� �� ate,c Tires. 2.Rpceiyed bj ate . Time
3.Relingi d b 3.R iv{ed b � � � DateTime
c l _ r2}I Dg�527/u cYI a5
4.Relinquished by Date Time 4.Receiiuea'by Date Time
0 Continuation of Custody on attached sheet 0 Received on ice(Circle) Yes No Ice Pack SC Receipt Terre] gc
DISTRIBUTION:WHITE-Return to laboratory with Sample(s);CANARY-Field/Client Copy Document Number:SCF-TS-248 Rev.3 Effective Date: 6/1/2017

Shealy Consulting,LW Page I of 3
Document number.SCF-TB-322 Replace Date!Oil 1 13
Ransgon number 2 Effective Date.Inv 2 I4
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis bahia
EPA 821-R-02-012(METHOD 2007.0)
CLIENT: j/< /).e r' [� TP
SAMPLE LOCATION:/// ErP/(.c_e n
TEST CONCENTRATION (IWC): 9L) %
Test Start Date/Time: 8'a7-JQ0 15o) Test End Date/Time: 33 / :t /2 f 5
Test Organism Age: 3.al y Test Organism Source: ri L Temperature of Organisms at Initiation: 024- l
Artemia Lot Number Tac - L 51 Salt Water Batch:,3/A J0$/9ao- 5 Salinity: ,.,?5 %o Incubator#:tin/vie
Date/Time Fed prior to Test Set-up:B/ 2 Je2O '1 — Date/Time Fed Via? 24 hours: /v / ►4}
I.2.5Q Ewl to z1/2.J ✓
Test Conditions(Check if in compliance,describe non-conformity if not):
Test Solution Volume: 200 mL .Test Chamber Volume: 250 niL • #Mysids/Rep: 10• 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
DATE/TIME AT TOX LAB
To i Q - g'02i/ i r�U ENTIRTHROUGHOUTE.TEST �ix.7/e2O a
5100
Test Initiation 24 Hour Reading Test Termination
Analyst ��)_)1' T
Date 81 ,21P0 e/a 12_0
Time 1500 1500
Aerated at
Start? Ilow
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-Farber or Prohit
48 !lour LC50: 95% Confidence Interval: -
COMMENTS: P5S

Shealy l:mwlhng.LLC Path:2 of)
Document number SCE-TB-322 Replaces Dale 10'1 1 13
Revision raunher 2
Effective UatC.Ow'_t'1 I
Client Name: l .be rit IAJT-r C, akte,4 jSample ID: 3-01g4 Initiation Date: 5/2 t/2C 2a
ACUTE SERIAL DILUTION TEST for Mvsidopsis bahia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
24 Hours 48 Hours .7L/9 Hours
Control A 10
B 0 0 /
Ac n
Rp 0
90 ;. B o 0
ls \
v
A
A
B --_
A
B
A
B
WATER CHEMISTRY DATA
Temp. D.O. pll Salinity Temp. D.O. pH Temp. D.O. pH Salinity
Treatment (CC) (mg/L) (SI) (Ppt) (°C) (mg/I.) (St') (°C) (mglL) (SC) (ppt)
SM2550B 55145000C SM150 Oil B SM2520B 5M25509 SM1e000C SM15001111 8M255011 SM45000G SM1500HB 551252011
0 HRS 0 FIRS 0 FIRS 0 FIRS 24 HRS 21 HAS 24 FIRS 48 HRS 48 FIRS 48 FIRS 48!IRS
Cando l e 4. I 7. 61 8 .2 2'9 :;t/. j .7 S 4
qoi .241. / 1.1,1 eg. 0 25..2 a4 1 '-1_50 .7.9
IX)\leter# 06E1533 OR Ft1 p11 SteterN120200067017I 132612567033 Thermometer/ 3 10 50 3 Conductivity Meter*/ YS I fr.. 30
Comments:

ihcal>•+'•alu�nng,ILL
Pytc?ol'3
Docwnan nundut:sCF:rR. 22
kcplace. Dale 10'I I I.i Ravnn+n number 2 }
I?Ilechvc Dale O0•1 /Id
Client Name: M1,t/�2✓ W TP trk1s*Sample ID: Sole' Initiation Date: id 1/J43
DATA VALIDATION:
LEVEL I: LEVEL II:
I)Stats input correct? Yes No
CONTROL VALIDTION: 2)Does test pass? Yes No
Mortality<10% ✓ 3)If test failed.has client been notified? [ Yes No
4)Date of notification: f1V
ADDITION VERIFICATION: 5) Is DMR attachment correct? Yes No
Initials of mortality calc: 6)Is test cover sheet correct'? Yes No
Initials of 2"d mortality calc: - 7)Is dilution series 0.5 or greater? Yes No
Data input requirements met? ✓ 8) If there are any protocol deviations.are they discussed Yes No
All sample holding times met? ✓ in the Case Narrative?
Sample information correct(see COC)? LEVEL II by
Temperatures in range? ✓
LEVEL I by: t '7"

Shealy('onsulhng.LLC Pagc I of 3
Document mmber.SCF-TB-322 Replaces Datc 10/I 1/13
Revl.wnnumbet 2 Effective Date 0o'25i14
SHEALY CONSULTING, LLC.
JS-HOUR ACUTE SERIAL DILUTION WITH Mysidapsis bahia
EPA 821-R-02-012(METHOD 2007.0)
CLIENT: Lt-h Q r L 2 l P
SAMPLE LOCATION: J r I rl c,1 c r — -J ins(;v elu.al samp&n
TEST CONCENTRATION (IWC): (10
Test Start Date/Time: iik2 7 kt c 1515 Test End Date/Time: 8/.2.t:3 J, IS i 5
Test Organism Age: 5 _ Test Organism Source: I Temperature of Organisms at Initiation: 0/4 /
Anemia Lot Number:Tao--4 5-4 5 I Salt Water Batch:.5 L.-)0 5 IQ i O-a5 Salinity: a 5 %o Incubator#: /47lne.ic
Date/Time Fed prior to Test Set-up: g/021/av jv95 C7 Date/Time Fed ret 24 hours: N► Pt
Test Conditions(Check if in compliance,describe non-confonnity if not):
Test Solution Vohune: 200 nrL •Test Chamber Volume: 250 mL . #Mysids/Rep: 10• 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
DATE/TIME AT TOX LAB
:TO 15 S H (;
a.J O 1�9 5/2&lava° T E:ROCItvTIRE TESTHOUT 61/.27.'/.24.1 goO f�
Test Initiation 24 Hour Reading Test Terminate
Analyst Crr:.e, ?T
Date B/a 7/e2L).. 4 ' fg 0 t'3/a2 TJp-40
Time 15 1.5 1515
Aerated at
Start?How
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-Karber or Probit
48 Hour LC50: 95% Confidence Interval: -
COMMENTS:
LiCL .7- Pa55
4 6 ss
LG _ (o 5S
qD RuI
e Pas5

Shealy CormInng.LLC. Paine 2 or3
Document number SCF-TB-322 Replaces Uate.10/1 1,13
Revision number.2
Effective We Otu25,i:1
31,165 —
Client Name: ti-u_19i, - Li) iP "nib 4i to'/'Sample ID: Jos Sq Initiation Date: p / o.-/ /o14),/-fc1
ACUTE SERIAL DILUTION TEST for Mrsidopsis bal:ia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead °% Mortality
24 Hours 48 Hours .2#1 Hours
Control A
B I 5 !.
B 4 l 0 1
A
: / 1
`16 B 0 C)
A
4 C B C S "�
Li C A In
o0
B /0
II.
A C'
1—
A
B
A
B
WATER CHEMISTRY DATA
Temp. D.O. p11 Salinity Temp. D.O. pH Temp. D.O. pH Salinity
Treatment (°C) (mg/1.) (S(") (PP() (°C) (mg/L) (SU) (°C) (mg/L) (SU) (PP1)
5M25508 S311500(X1 55145001(13 SM252011 S51255011 SM15000C SM4500HB 5M255011 SM4S000G SM450011B SM25208
0 FRS 0 FIRS 01-IRS 0 FIRS 24 I IRS 24 I IRS 24 FIRS 18!IRS 48 IIRS 48 FIRS 49 FIRS
0011 I ail. 7.&• A,Q .24 8 074.0 3.4I-3 9. 2
14 a-1 I 7.7 s c I .2'.3 024 o 7. lio g 3
8 0,1.3 7.14 ' I 2'/.4, ad 1 7- 55 8.d
C 24 3 7.?8 E. .. 02q. I e24. 1 -7.-7o V . g
1) .L 3-• 7.4 e2&.i (94 .7 -t.3a ylr
E- ay. I f.9D 8, 2_ a 5. 2 a q. i 7, e y s. 3 /gyp
DO Meter 9 06E1533 OR 1134 pi1 Meter 9J20200067017/132612567033 Thermometer 300 50.3 Conductivity Meter,/ `/'S1 T P8.030
Comments:

Shealy Unsmiling.LLc'
Docvmenlntenbcr SC'F•TI3-322 a
Replaces Dale
10 of3 0lI 1 1 13
Revision number 2 Effective Date OoI2S%I I
-so iW5—
Client Name: kJ i)( , d J_1)-1/„( "4 Sample ID: TO 115i Initiation Date: 6h / .
DATA VALIDATION:
LEVEL I: LEVEL II:
I)Stats input correct? Yes No
CONTROL VALIDATION: 2)Does test pass? Yes No
Mortality < 10% 3)If test failed,has client been notified? Yes No
4)Date of notification:
ADDITION VERIFICATION: 5)Is DMR attaclunent correct? f\I Yes No
6)of I" mortality calc: r.....)1 Is test cover sheet correct? Yes No
7 Is dilution series 0 5or ?�
Initials of 2"'mortality cafe: — > d o se s greater? Yes No
Data input requirements met? ✓ 8) If there are anN protocol deviations.are they discussed Yes No
All sample holding times met?
in the Case Narrative?
Sample information correct(see COC)? LEVEL II by:
Temperatures in range? -
LEVEL I by: EC,4)-1

Shealy Conu 1ttrtg.LU' Paige 1 ar3
Replaces
Document number:SCF-TB-322
Date 10111,13
Revision uaunber-2 Effective Wit Oui/25/1!
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis bahia
EPA 821-R-02-012(METHOD 2007.0)
CLIENT: /11AlJrt r V T f
SAMPLE LOCATION: Jpj; , rt:Vi i)- (pn - -7 - b 1
TEST CONCENTRATION (IWC): gip %
Test Start Date/Time: 8/J1/02. 15,25 Test End Date/Time: 8/a 9A-' 15 a5
Test Organism Age: 3 d wrest Organism Source: A 7 Temperature of Organisms at Initiation: 07 4•
Anemia Lot Number: TAD. 45) Salt WaterBatch:.5t✓L►S/Gv?O j Salinity: W5 96. Incubator#: Isac
Datc/Time Fed prior to Test Set-up: /.. 7/ow Ia5D Date/Time Fed(it.24 hours: /4
Test Conditions(Check if in compliance.describe non-conformity if not):
Test Solution Volume:200 mL •Test Chamber Volume: 250 mL • #Mysids/Rep: 10. 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
DATE/TIME AT TOX t.AB
TO190- THROLIGFIOUT
J 01 5 3 fY/ / ENTIRE TEST E3/ r�J-ow 0gc25
Test Initiation 24 Hour Reading Test Termination
Analyst L4Ild C [
Date 6 la-7 Po 43'a35/_
Time 15a 5 15.25
Aerated at
Start?How -_
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-liar'ber or l'robit
48 Hour LC50: 95% Confidence Interval: -
COMMENTS: pas,

Shealy Consulting.l.l.t' 2
f 3
Document number SCF.PI1•322 Page .12
Replies Date'IOiI 1:11
Revision mother 2 Ft1eenve hate 06/25/I.l
L� J 0 191c -•
Client Name: >4 to hri'i Sc(i;rill-r)'LS Sample ID: TO)93 Initiation Date: g(c2 7/a-J L '
ACUTE SERIAL DILUTION TEST for Mvsidopsis bahia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
24 Hours 48 Hours di/48 Hours
Control A C
B �� 0
5 B A
ri C)
A C 5,/
<< B r
A r
B 0 C)
F A l
B
e I S. /
A
B
A
B
B
WATER CHEMISTRY DATA
Temp. D.O. pH Salinity Temp. D.O. pH Temp. D.O. pH Salinity
Treatment (°C) (mgll.) (St) (ppa (°C) (mgli.) (SI') (°C) (mg/l.) (Si") (Ppt)
8M25501) SM4S000C SM4500118 SAI252011 8M2350B SM.5000C S1%1.1500116 SM25506 SAI4S000G S545(101113 851252011
0 FIRS 0 HRS 0 FIRS 0 HRS 24 HRS 24 HRS 24 FIRS .18 I S 48 IIRS _48 FIRS 48 HRS
COMIC a44 I 7.bI s.a a4.8 074 a ' .g'/ 8 3
5 a45 569.7 43. 2 .../ , Qia 7•-29 g. 3
(r 251 5.8li V 1 a,-/.2 .2'1- l 7 33 te .ik
7 5 a/ 95 t,0 ?LI a V N o 6. 9a e 1
v -2-1. 5 5. 31 -7. 5 -74.L{ WN.a 4 /9 )
DO Meter S 11010533 O FI IS pH Meter 8120200067017/(326125(7033
Thermometer 8 3 I503 conductivity Melerli .► / .0 A _-
Comments:

Shealy Cotwt@tag,LLC Page 3 of 3
Document minim-SCF-TE1-322 Replace::Date 10/11,13
RCOI,VPn uwttbcr 2EIT•cnrc D'ule.0o125rI I
1 rrt _9 TOt
/j,r -
Client Name:� � r Ara'1 ... / Vert 13' Sample ID: J D/g.3 Initiation Date: ¢!/J
DATA VALIDATION:
LEVEL I: LEVEL II:
l)Stats input correct? Yes No
CONTROL VALIDATION: 2)Does test pass? Yes No
Mortality < 10% ' 3)If test failed,has client en notified'' Y N
4)Date of notification: / •
ADDITION VERIFICATION: 5)Is DMR attachment correct'? Yes No
Initials of I"mortality calc: CL,r 6) Is test cover sheet correct? Yes No
„r 7) Is dilution series 0.5 or greater? Yes No
Initials of 2t'd mortality calc:
Data input requirements met? ✓ 8)If there arc any protocol deviations.are they discussed Yes No
in the Case Narrative?
All sample holding times met? ✓
Sample information correct(see COC)? LEVEL II hy:
Temperatures in range? ✓
LEVEL I by: EUji

APPENDIX D
AUGUST 31, 2020
TEST DATA

SHEALY CONSULTING, LLC t42
SHEALY,-4 CHAIN OF CUSTODY RECORD
.-- CONSuuiNG.uc 343 W. Columbia Avenue• Batesburg-Leesville,SC 29006
Telephone No. [phone] I
www.shealyconsulting.net Page of
Requested Analysis
Client , n
Address
C,ty State Zip Code
Preservaivc
Project Name ✓ — )� I Cnpres. 5.HCL
I ;'t / /��,K. , C r 2.NaOH/ZnA 6.Na.7hio.
t 3.H2SO4 7.NaOH
Sampler Name Phone a Bottle Size: tic 1 4.HNO3
Preservative Codes: k
a _ ,
Sample ID/Description °o�°'' Sampler Start Sampler End Sheaty Lab ID
Indreate number of containers in the boxes below.
(Containers for each sample may be combrnev on one line.; Date Time Date Time
EIT/Li E n 4- /1 13 5--3 1 Gov
�" `3-- 3I 0b3LI J oi9,4
7.Relinquished by Dare Time IT ed by Date Time
s....,)csi
4 6P-3/-.),-, lees-5-
2. RzuisklQ,d by / ) (�at� time 2. eived bX• /� Date� Time
t ' ,��� <• 0/' � ; L r` ,)c .�� I l c I2_uN . •l-; C aC I ca-\
3.Relinq&l�'aed by Dat Time 3. eived by Da a Time
J C Jfle.c.4-t 1�se_a.._ 9/1120 0 9,SO _ ( 01-11,L.D. Jh 2s,+^--' _4/1/ZO , 0930
4.Relinquished by Date Time 4. e ed by Date Time
jIMIIIIIIIIMIMIIIIIINImimE
0 Continuation of Custody on attached sheet 0 Received on ice(Circle) Yes No ice Pack SC Receipt Temp, 'C
DISTRIBUTION:WHITE-Return to laboratory with Sample(s):CANARY-Field/Client Copy Document Number:SCF-TS-248 Rev.3 Effective Date: 6/1/2017
L

SHEALY CONSULTING, LLC No 4542
SHEALY 0 CHAIN OF CUSTODY RECORD
ON$ULTING.LLC 343 W.Columbia Avenue• Batesburg-Leesville,SC 29006
Telephone No. [phone] ,
www.shealyconsulting.net Page • of
Client Nt.he r f ( 3-1-_
Address 1
1.9
City State Zip Code ''1�
LP Preservaive
Project Name 1.Unpres. 5.HCL
T 1-- 2.NaOHfZnA 6.Na.lino.T L /0?C 1 �'! L2 3.H2SO4 7.NaOH
Sampler Name Phone 4 Bottle Size: r2- 4.HNO3
Preservative Codes: J`
Sample ID/Description c°^'0'' Sampler Start Sampler End
Indicate number of containers in the boxes below.
(Container;for each sample may be combinerd on one line) srac Date Time Date Time
} r"Y_3/ Oft z <564 S44.44.64
.�r, •'1L QnD L} o G 4-4-- 0700 I 3—oI95
in-c1t,enIL- 46 L+ . , ' , Izs" NO ',tote ") I Jo19(0
_Ln 4 I L,cerr Li-C C 3 i l3iz it-
cs`z Co' To r9
--1-n .-r 1,...ce n G. 'S 3 I (SO 1es.'-'11' $ ►'(
1Lrs-- ( Jof48
.Lncf(A. )-- 1- Lie G q3f l(",c(� 5ff 57 1 To I 9 9
1.Relinquished by l l� Date Time 1. c by • Date Time.
2. uished te, e T 2.�eceive�by Date Time i
mac--- 1' I-,� 'y 4 �� )r ; r��� 5, 1-;<.b d L%
3.Reli uished b�c Date Time 3.Re ved b
Jo c c A rte. q/ i 110 0 9 34 i.c.�. j�,7p i- q�t]� Time 0 9 3 R
4.Relinquished by Date Time 4.Recejd by Date Time
❑ Continuation of Custody on attached sheet 0 Received on ice(Circle) Yes No Ice Pack SC Receipt Temp•I C
DISTRIBUTION:WHITE-Return to laboratory with Sample(s);CANARY-Field/Client Copy Document Number:SCF-TS-248 Rev.3 Effective Date: 6/1/2017

Shealy i'.autdtlnk.tic F'tbe I of)
Ikictunent number SCF•TB.322
Reviunnnunber2 Replaces ctiveDatc.lo/lI/I3
Effective Date Ou4N17
SHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis bahia
EPA 821-R-02-012(METHOD 2007.0)
CLIENT: f/1<.ber/ GJTe
SAMPLE LOCATION: PPIulnT - toCcAcr 1
TEST CONCENTRATION IW • qD %
Test Start Date/Time: q' I/01023 /y 3- ) Test End Date/Time: We:2 A D&O (5 0,5
Test Organism Agc: 3C1'ct Test Organism Source: AI Temperature of Organisms at Initiation: 211. 9
Anemia Lot Number T20-N 51 Salt Water Bat
ch: Sw O 819 20-.? �5 %o Incubator#:
5 Salinity.:►� Annex
Date/Time Fed prior to Test Set-up: 9/1/x .v /ca.)30 Date/Time Fed ra_�,24 hours: N 1 A
Test Conditions(Check if in compliance.describe non-conformity if not): ✓
Test Solution Volume: 2(K)mL •Test Chamber Volume: 250 mL • #Mysids/Rep: 10 . 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED D:VI•E/TIME RECEIVED
DATE./TIME. AT FOX LAB
TtlROUGHou'r T ID q4 S f 31120 08 ZO 0O ENTIRE TEST (]q CI
Test Initiation 24 Hour Reading Test Termination
Analyst f4.0 f�Jz
Date �� �a0. _0 9/aJa.O cgs-)
Time /4 • ° 1.5-0.5
Aerated at
Start?How
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-Karber or Probit
48 Hour LC50: 95% Confidence Interval: -
COMMENTS: — / `), S S

Slwaly Conanlong.LLC Page 2 of
Document number SCE-TR-322 Repla xs Date:I011 1 1.1
R wnk n number 2 EO'a uve Date 06'25,I4
Client Name:fluber¢ WTe Ef'f'Itten# Sample ID: O1cw Initiation Date: 9 / 1120
ACUTE SERIAL DILUTION TEST for Mpsidopsis hahia
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
24 Hours 48 Hours 24 44 Hours
Control A `''
B O c
C e)
90 /. B 7, 5
/ --
$ C I
>1' D 0; —
A
B
A
B
A
B
A
B
WATER CHEMISTRY DATA
Temp. 1).0. pll Salinity Temp. D.O. pIt Temp. D.O. pH Salinity
"Treatment (°C) (mgiL) (St') (PP)) eC) (mg/1.4 (SI') (°C) (mg/L) (SU) (ppt)
S11125508 SM4501,0(: SM1450011B SM252011 SMISSOR 53,1450000 S13145001111 SM2ScoIl SM4S000G SM150014B SM25201)
0 FIRs 0 IIRS 0 HRS 0 HRS 24 HRS 24 HRS 211IRS 48 IIRS 18 FIRS 48 HAS 48!IRS
Con+►o I .25S (2,8 8, O 94,q e2 .3 .7t a 8.0
9o% a5.-1 -7. I 8 ,0 0,-I.8 .2460 '7. 3 F . I
" 3.Et.tir V fi/u6
DO kleter 8 06F:1533 0 05F1131 p11 Meter 01211200uo7u1 7/132012567033 Thermometer 8 310503 Conductivity‘letcr8 Y�3b
Comments:

Shealy Cbmulung.LLC Pegs 3 of 3
Document number:SCF-TB-322 Replaces Dale 10/11/I3
Revision number Z Effective Date-06R5'1.I
Client Name: I- kerb E fl( Utn4 Sample ID:T0I qi4 Initiation Date: 20
DATA VALIDATION:
LEVEL I: LEVEL II:
I)Stats input correct? Ycs No
CONTROL VALIDATION: 2) Does test pass? Yes No
Mortality< 10% 3)If test failed,has client been notified? k / 141 Yes No
4)Date of notification:
ADDITION VERIFICATION: 5) Is DMR attachment correct? Yes No
Initials of I"mortality cafe: J7 6) Is test cover sheet correct? Yes No
Initials of 2"d mortality cote: 7) Is dilution series 0.5 or greater? Yes No
8) If there are any protocol deviations,arc the\ discussed Yes No
Data input requirements met? 1/ in the Case Narrative?
All sample holding times met? ✓
Sample information correct(see COC)? ✓ LEVEL II by:
Temperatures in range? ✓
LEVEL I by: L .)1

Summary Sheet
Ficd.Iy Huh.,r' Analyst EWT
Test ID Sae 40 Specter. Amervamysrs bate.imysd sh'Iny01
Date 9r12020 Test Type Acute Sunman
Lee ID Shanty
IWC Conc 90
Input
Number of Orgarvsms Eepaed or Counted
Concentrabocs
Repfteate Q 99
I 10 10
2 10 10
10 10
4 10 l0
Noma.,ul Optsnnms Stinson('or R.sp0mmrtd
Coocanlrenpns
Replcale 0 90
1 10 10
2 10 e
3 10 9
4 10 10
Total Oroanisms 40 40
Total Responcenp 40 37
%Responding 100 016 92 5
Output
Staleeca'Data Conc Mean(Tanslolmed) Mean Shim CV I"lost
v.a.r.,..suss anew pea•....am,,,y a.+a l.� 0 +4120181t2 1 0 n000
pan...".ea.ten r. 90 1 295056670 0 925 0 146953 0'13 NS
•row,'ur:vae., NA
O..e..s rAnsgsn.an.p•nu NA
NA
l.ttr, NA
40.,.a..v .e r.a NANA,a so.e:n NA
real Accepteetley Cute,..
Crawler, Test Value Pass/Fad Cntenon Teal Value Pass/Fail
Control mean +=09 I sass
N4 NA NA
respoe NA I
ns NA PUSS
A NA NA
CGwmt CV NA 0 NA MS0 NA I.A. NA
SAC Flaps No rap.
Hypothesis lest T-Test with Bpnler'on•Adluslrnenl
Point estimate lest REF
NOEC LOEC _ LC50 95%Confidence Intervals
90 •90 'MEP "REP .REF'
TST Calculated 1-value Table l-velue Retattve%Effect at IWC
Peas NA NA
750
Note For slatisecel tests'NS-'notate,that the nencentlatrnn•,nil slebslrcally t.lfwpnl from me Mnlrol while m rn.),_ales that the cona,mlaeon
n statsacally Olerent from the cannot
Toxicity Test Results Cnrvo Type I
I:00
,lad _
g.sat
30e00
i
0.400
0 200
0 90
concenrrNwns
NOTICE -" --
The Ln too Stales Environmental Protection Agency(EPA)Through Os Office of Waslewaler Management,!unclog and managm2 Ine
development of the whoto effluent toxicity(WET)Tool descnbe0 here This is a tool that calculates WET test endpoints for the EPA-
aooroved WET lest methyl.and is used by FPA.eternally for analvano valid WET lest data Neither the FPA nor any of then
9,32020
Pane I nl 1 wet anatyl•calspraansnc.l 20190910

S11,1y ..aaritmg.Ll.c Page I 43
I e+cumstl number SC P•TR-322 Replaces Dale 10eI 1,1
K n•+v.m number 2 IitTecttve Date 01e 2s I I
SIHEALY CONSULTING, LLC.
48-HOUR ACUTE SERIAL DILUTION WITH Mysidopsis bahia
EPA 821-R-02-012(METHOD 2007.0)
CLIENT: Ell tint r4 L`)Tr�
SAMPLE LOCATION: I Lie n'i - JQrryi Loe&I,'ern
TEST CONCENTRATION (IWC): p %t 'r
Test Start Date/Time: c Ji/:)C;J a 1L L C) Test End Date/Time: 9�'2/.ay.2C) 1 5—l0
Test Organism Age: 3 do.f.Test Organism Source: ►41. Temperature of Organisms at Initiation: 024. 9
Anemia Lot Numbed-20 -LI S i Salt Water Batch: ed 08 Oa)-25 Salinity: 25 960 incubator#:rl nnex
Date/Time Fed prior to Test Set-up:d/I'a02JD r(1 3O Date/Time Fed a 24 hours: w I 0
Test Conditions(Check if in compliance.describe non-conformit) if not): ✓
Test Solution Volume: 200 mL •Test Chamber Volume: 250 mL . #Mysids/Rep: I0. 16 Hour Light/8 Hour Dark Cycle
SAMPLE USAGE:
SAMPLE ID COLLECTION WHEN USED DATE/TIME RECEIVED
DATE/TIME _ AT ToX LAB _
3o IMS - THROUGHOUT
20199 $13 `20 ENTIRE TEST 411120 0Ct3,2
Test Initiation 24 Hour Reading Test Termination
Analyst f ')7 &JD/
Date l�//°2 v:20 9/a/Jo
Time /Lig0 ( 510
Aerated at
Start? How --
Long?
RESULTS:
Statistical Analysis Used: Toxcalc 5.0 Trimmed Spearman-Karber or Probit
48 Hour LC50: 95% Confidence Interval: -
COMMENTS:
Z71- - Feu1
y - Pass
yc - Vass
r f -
24 E, - Vass

Slimly(imudmg,LLC Page 2 of3
Weinman munber SCF-TR-322 Replace:.Nate.10/11/13
Revuann number 2 Mann Date 06r25/14
To195-
Client Name:(-1t►her4 Lore Inch n+ Sample ID:70199 Initiation Date: 9/120
ACUTE SERIAL DILUTION TEST for Mpsidopsis hahi«
DAILY MORTALITY and WATER CHEMISTY DATA
Treatment Replicate # Dead # Dead % Mortality
24 Hours 48 Hours 2' %Hours
Control
B 0
A 1 n
L B I /CAL i
A
y 6 B
A
Li C B I 5
A I
Li D B 1 (7) / Ln
A Q
LIE B i S"
A
B
A
B
WATER CHEMISTRY DATA
Temp. D.O. pH Salinity Temp. D.O. pH Temp. D.O. P11 Salinity
Treatment (°C) (mg/1.) (S11 1ppt) (CC) (mg/L) (SC) (°C) (mg/'I.) IS1.) (PP()
S\125506 SM45000G S\11500HR SM252011 S\12550I4 S\U5000G S\71500HB 551255013 S3i 15000G S5145001111 SN1252011
0 I ntS 0 IIRS 0 IIRS 0 IIRS 24 ORS 24,11 24 MRS 48 IIRS 18 FIRS 18 ORS 18 IIRS
Con I d4.9 (o,b 8.4 ( .9 '25a 1 qli'O L3
4A a5 0 -1 .4_- i . I I -.4. a •3-.
46 a.5 5 (ski 8., ail, 3 e>z s ' -4-. a g. 3
&lc 21{9 -7, e. a4.o a5. a 7, g 6.3
7. 8
Lie a55 (4).-7- 9 . 9N 07'4 A 024.9 }. a 8.2
IH)Meter# 068.1533 OR 7333 pH Meter N1 2 0 2 0 011671)17/132612567033 Thermometer II 310 503 Conductivity Meter# YS I Pro 30
Comments:

Shealy('attauIOng.LLC Putty l of+
Document number SCF•TR-322 Replaces Date I011 l I3
Revision number 2 Effective D. ,00:25;I
II J TOr95-
Client Name: T1 u�Gr 4 LiTP -Tn-1!uer Sample ID: To I99 Initiation Date: ct I/ZD
DATA VALIDATION:
LEVEL I: LEVEL II:
l)Stats input correct? Yes No
CONTROL VALIDATION: 2)Does test pass? A r , �� Yes No
Mortality < 10% �/ 3)If test failed. has client been notified? a ( 4 _ Yes , No
4)Date of notification:
ADDITION VERIFICATION: 5) Is DMR attachment correct? Yes No
Initials of I"mortality calc: 1 6)Is test cover sheet correct? Yes No
Initials of 2'mortality calc: - 7) Is dilution series 0.5 or greater? Yes , No
Data input requirements met? ✓ 8)If there are any protocol deviations,are they discussed Yes No
in the Case Narrative?
All sample holding times met?
Sample information correct(see COC)? LEVEL II by:
Temperatures in range? ✓
LEVEL I by: (J)

APPENDIX E
TEST ORGANISM REFERENCE
TOXICANT TESTS

UAT Control Chart-I
Control Limits for Standard Reference Toxicant Tests
�� ACUTE•••Mysidopsis bahia(conducted by Hydrosphere Research)
Q/NDICATOt...
1000
950 — ��- y� -�LC50
900 — The LC50 iswithinthe ntrol limns -AVG
850 — t -h.Upper Control Limit
QA Signature �JALh �4 Date:09/02/20
✓ -x-Lower Control Limit
800 Note:Dates with no corresponding LC50 data point,if present on chart,indicates an invalid test
Note:If the control limit(s)for two or more consecutive tests are exceeded then the results must be
750 — explained here and the test must be repeated immediately(EPA-82I-R-02-012,Sections 4.15.3
&4.15.4,page 18). Also,Section 4.15.2 of the same Method states that"...reference toxicant
700 —
r 650
vni 600
0
-� 550
500 +— +�- +�--+ - ' + + I�" +—+—w
e 450
s' 400 ---
350 ��
300 —
250 -- 1
200 --
150 x"---.•...x x x x x x,....--.-•x""'""x— x-----'
100
50
0 I I I I I I 4 I I I 1 I I I I I I I
2ir '''ir rib r- 6i5, i/ 4 -riy /0- //� �Z /ia. 1i, �-' �'i� Sir 6i/ er: 2 d?-
0/9 /�%D �%D 4t) -%fl -%9 /�%9 /2%O r/��/9 r/��/9 k2�/9 ���0 '% ' 0 °--, -0�� -0?, 4,-,,, �0�� /si20
Test Dates

''�+ Control Chart - II
��UA L jC "�4 Coefficient of Variation for Standard Reference Toxicant Tests
� ACUTE Mysidopsis bahia(performed by Hydrosphere Research)
-INDICATORS
0.500
0.490 -
0.480 - - CV= 0.26
0.470 —- -- The CV is less than or equal to the national 90th percentile.
0.460 - -
Comments(if neededl•
0.450
0.440 - - QA Signature: te: 09/02/20
0.430 -
0.420 -
0.410 - -0-CV tNational 90th%
0.400 -
• 0.390 -
2s 0.380 -
• 0.370 -
O 0.360 -
O 0.350 -
-ff
v
• 0.340 -
0.330 -
0.320 -
00 0.310 -
n 0.300 -
< 0.290 -
0.280 -
0.270
0.260 • • O O • • •
0.250 -
0.240 -
0.230 -
0.220 -
0.210
0.200 r t I I I I I I I I I I I I I I I I
L .2� Yi Y� tTi 1i dsi -0 ! ! ! !i .2 .2 .2i .P� 6i 6i c- 8�
Yi Y d� c� J� !� .f� 9� Oi !i 6� .?� .� Yi i • .2
2019 ?0!9 S.30 9�0l9 0l9 2'0 C.la 0!9 �Ol9 �0l9 0!9 020 10c0 0�0 O�0�0 ?0c0 ?'0 9 010 20 0sS0
Test Dates

REFERENCE TOXICANT LOG.
Test: 48-hour Acute
Species: Mysidopsis bahia
Aquatic Indicators, Inc. '"% ;'"'
Vendor: Aquatic Indicators
"Toxicant: Copper Sulfate(}tg Cu/liter)
Lower Upper
N DATE LCSO AVG S.D. 2 SD +2 SD -2 SD CV National National Control Control
75th Y. 90th% Limit Limit
406 2/4/2019 532 345.53 99.77 199.54 545.07 145.99 0.27 0.26 0.26 165.85 525.21
407 3/4/2019 472 356.94 100.60 201.20 558.14 155.73 0.29 0.26 0.26 171.33 542.54
408 4/8/2019 357 346.97 88.97 177.94 524.91 169.03 0.28 0.26 0.26 166.55 527.39
409 4/29/2019 436 350.91 91.15 182.30 533.21 168.60 0.26 0.26 0.26 168.60 533.21
410 6/5/2019 277 345.62 92.26 184.51 530.13 161.11 0.26 0.26 0.26 161.11 530.13
411 7/1/2019 244 338.22 94.25 188.50 526.72 149.73 0.27 0.26 0.26 162.35 514.10
412 8/5/2019 240 326.62 91.16 182.31 508.93 144.31 0.28 0.26 0.26 156.78 496.46
413 9/9/2019 331 329.30 90.40 180.81 510.11 148.49 0.28 0.26 0.26 158.06 500.54
414 10/4/2019 277 320.76 86.59 173.18 493.94 147.59 0.27 0.26 0.26 153.97 487.56
415 11/4/2019 345 325.62 85.00 170.00 495.63 155.62 0.27 0.26 0.26 156.30 494.94
416 12/2/2019 271 325.72 84.94 169.87 495.59 155.84 0.26 0.26 0.26 155.84 495.59
417 1/6/2020 229 321.95 87.58 175.17 497.11 146.78 0.26 0.26 0.26 146.78 497.11
418 2/3/2020 308 317.19 85.48 170.96 488.15 146.23 0.27 0.26 0.26 152.25 482.12
419 3/2/2020 332 317.54 85.53 171.06 488.59 146.48 0.27 0.26 0.26 152.42 482.66
420 3/30/2020 290 315.94 85.74 171.48 487.41 144.46 0.27 0.26 0.26 151.65 480.22
421 5/4/2020 411 318.89 88.01 176.02 494.91 142.86 0.27 0.26 0.26 153.07 484.71
422 6/1/2020 416 329.08 86.84 173.68 502.76 155.40 0.28 0.26 0.26 157.96 500.20
423 6/29/2020 262 331.90 83.46 166.93 498.83 164.97 0.26 0.26 0.26 164.97 498.83
424 8/3/2020 244 330.55 84.71 169.41 499.97 161.14 0.25 0.26 0.26 161.14 499.97
425 8/31/2020 366 332.03 85.08 170.16 502.19 161.87 0.26 0.26 0.26 161.87 502.19

Aquatic Indicators,Inc. Acute Saltwater Method(EPA-821-R-02-012) 47
P.C)Hon 632-St.Augustine,Fl.32085-0632-[phone] Method#2007.0&SOP TST-003.02 '
Survival
EeSi I� /
RT for the month of: �ccSRT for the Client: Control Water: SSW Initiaton Date: B � s 1 / Termination Date: ` /7`2�
` {)--ejOiR Toxicant(desiccated): Cur-,desiccated
Species: Mysidopsis bahia Code: MS Test Vessel: 500-mL Plastic Cup Stock Solution(Concentration): 0.1-g Cusn/L
ID IF: I D�1 Age:Sek 0 Lab,®Comm Test Volume: 200-mLs per replicate Test Concentration(Units): µg WI L
r Toxicant Use Counts pH Dissolved Oxygen(mg/L) 1 Salinity a2W
Vol. (A -I— w p
ofstoek Copper evoretem�xea: valid whsebs) (.ewuu�dan. re,..rie baa9.o.a4.)
Isxrsarrmdaueamkt:l
/400-mLa OWL) �i Oh 24h 48h Oh 24h 4 Oh 24h 48h --- --_
new old soluton old solutionPR
Control 0 A 10 1() 10 (.J} 1.6 -7 E I 41 ( 3
B 10 10 l� !'
r
A 10 rG 1' P. — 0 ( _ r
o.22-mL 55 B 10 /O to • 1"' .O Cr. lD.�—1 2
0.44_0. 1 to - - 10 ./u �0 �i /I .- -�7 b'U t,S
B 10 Ai 1/6� X� N.
0.88-mL 220 10 �( � ^`'6 -7.� N �1 L�
B 10 1G y(� 9� ,r
A )0 /7 1�3 l�-' --
L76-mI 440 e _SS. 13 I• �'/K" —!7 �x7 U �•t �`•�.
io
3S2-mL 880 B 10 0 8 a I �'� I`
Meter ID a: XXX 2.-/ (\ 2 ) 11 id 16�/ IS
Initials: mum r!1 n� ffY�/l`l WI 4 CI) �D
Time �� mi, w 1
r_ Notts&Comments I Temperature(°G�
,�a�a{{'yyy� o IV 'carmine. l�b i�1:' d�`P th • / a II__ _ 24 48
Control ID:-r.Z�r`��i- „��n� Control Mar�ltra
�rdiii7 , 1�
Toxicant Copper ID'�,1-Ly�mouton time. 55 ), �ippllI++��
Y 110 IAliil
Random- Fading Type: Artemis(concentrated slurry) Photoperiod is 16L:8D,Illumination is ambient(50 to I00 Red) 220 2 ,6 ��
Inalian
M
Amount: 2-drops(0.1-nL):-then.da0y ID Measured at the end of each 24-h exposure period,on one replicate. 440 ,5� ��
�s (acceptable range for a valid test it 25*I°C) 880 ��,���'il
Time JSUC 15L5 KNA
mCMdc Box:Lab-In-House Reared,Cans=Commercially obtained Meter IIM:
Version 2(13080•DCFI F:IBenchsheets,Logs&Formsll)BenchSheels11)ACUThtNPDESISRT\SRT.MS.48.ASND.EPA2007.v2g(AI)-

CETIS Analytical Report Report Date: 02 Sep-20 13:32(p 1 of 1)
Test Code: SEP20 MSA-Al 113-2482-9234
Mysidopsis 96-h Acute Survival Test Hydrosphere Research
Analysis ID: 17-5172-1955 Endpoint: 48h Survival Rate CETIS Version: CETISv1.9.3
Analyzed: 02 Sep-20 13:32 Analysis: Trimmed Spearman-Karber Official Results: Yes
Trimmed Spearman-Karber Estimates
Threshold Option Threshold Trim Mu Sigma LC50 96%LCL 95%UCL
Control Threshold 0 20.00% 2.564 0.05794 366.4 280.6 478.5
48h Survival Rate Summary Calculated Variate(A/B) Isotonic Variate
Conc-pg/L Code Count Mean Min Max Std Dev CV% %Effect A/B Mean %Effect
0 D 2 1.0000 1.0000 1.0000 0.0000 0.00% 0.0% 20/20 1 0.0%
55 2 1.0000 1.0000 1.0000 0.0000 0.00% 0.0% 20/20 1 0.0%
110 2 1.0000 1.0000 1.0000 0.0000 0.00% 0.0% 20/20 1 0.0%
220 2 0.9000 0.8000 1.0000 0.1414 15.71% 10.0% 18/20 0.9 10.0%
440 2 0.3000 0.2000 0.4000 0.1414 47.14% 70.0% 6/20 0.3 70.0%
880 2 0.2000 0.2000 0.2000 0.0000 0.00% 80.0% 4/20 0.2 80.0%
48h Survival Rate Detail
Conc-pg/L Code Rep 1 Rep 2
0 D 1.0000 1.0000
55 1.0000 1.0000
110 1.0000 1.0000
220 0.8000 1.0000
440 0.4000 0.2000
880 0.2000 0.2000
48h Survival Rate Binomials
Conc-Ng/L Code Rep 1 Rep 2
0 D 10/10 10/10
55 10/10 10/10
110 10/10 10/10
220 8/10 10/10
440 4/10 2/10
880 2/10 2/10
•
002 360 881-3 CETISTM vl.9.3.0 Analys.A !N QA:09/02/20