NC0004961_Regional Office Historical File Pre 2018

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Erw ronmental Testing Solutions, Inc.
Effluent Aquatic Toxicitv Resort Form - Phase Il Chronic Ceriodaphnia dubia
PO Box 7565
Asheville, NC 28802
Phone: [phone]
Fax: [phone]
Date: January 2i.'.019
Facility: Duke Energy Corporation NPDES #: NC- 0004961 Pipe #: 002 County: Gaston
Riverbend Steam Station
Laboratory Performing Test: Environmental Testing S-pWisn, lac. Comments:
Signature of Operator in Responsible Charge:
Signature Of Laboratory Supervisor:4gAdjW
Project: 13895
Samples: 190109.12, 190111.09
Mail Original To: North Carolina Department of Environment and Natural Resources
DWQ/ Environmental Sciences Branch
1621 Mail Service Center Start date: End date: Start time: End time:
Raleigh, NC 27699-1621 01-09-19 01-16-19 1450 0728
Sample Information
Collection start date:
Grab:
Composite duration:
- - - Alkalinity (mg/L CaCO3):
Hardness (mg/1. CaCO3):
Conductivity (pmhos'cm):
Total residual chlorine (mg/L):
Sample Temp. at Receipt (°C):
Sample 1
Sample 2
Control
01-08-19
01-10-19
x
x
33,32
34,34
236
221
156.152. 150
<0.10
<0.10
1.1
2.1
Test Information
Treatment:
Initial pH (SU):
Final pH (SU):
Initial DO (mg/L)
Final DO (mg/L):
Initial Temp. CC)
Final Temp. CC):
Organism Number
Control Orpanisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean
Sun
R-al1
Reneaal2
Sun
RencxalI
Rcnewal2
2.7%
2.7%
2.7%
Control
Control
Control
7.83
7.82
8.32
7.80
7.79
7.81
8.33
7.81
7.97
7.73
7.84
7.72
8.2
7.8
8.2
7.8
8.0
8.1
8.2
8.1
8.3
7.9
8.0
8.0
25.1
24.8
24.8
24.9
24.7
2-1
25.0
25.1
24.9
25.1
25.0
25.1
Number of Young Produced
126
29
30
32
32
29
30
31
30
29
31
30
29.9
Adult Survival: Live, (D)ead
I L
L
L
L
L
L
L
L
L
L
L
L
Effluent Percentage z.7%
Treatment 2 Organism., 1 2 3 4 5 6 7 R 9 10 11 12 Mean
Number of Young Produced
136
31
33
32
35
29
36
32
30
35
32
39
33.3
Adult Survival: L)ive, (D)ead
I L
L
L
L
L
L
L
L
L
L
L
L
-11.4
Effluent Percentage=
'reatment 3 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean
lumber of Young Produced
Ault Survival: (I.)ive, (D)ead
%Redwtic
Effluent Percentage=
'reatment 4 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean
lumber of Young Produced
idult Survival: (L)ive, (D)ead
% ReNKtic
Effluent Percentage=
•reatment 5 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 Mean
lumber of Young Produced
Ault Survival: (L)ive, Dead
%ReduGi[
Effluent Percentage=
•reatment 6 Organisms 1 2 3 4 5 6 7 8 9 10 11 12 mean
lumber of Young Produced
Ault Survival: L)ive, Dead
. RCOUCIWn
Overall Analysis:
Result: PASS LOEC: >2.7% NOEC: 2.7% ChV: >2.7%
DWQ form AT-3 (8191) Rev. 11195
Chronic Test Results
Final Control Mortality (1i6):
0.0
% Control with 3rd Broods:
100
Control Reproduction CV:
5.4
48 Hour Mortality
Control:
o
12
1WC:
0 +01f
12
Significant?:
No
Final Mortality Significant at:
No concentration
Reproduction Analyses
Reproduction LOEC:
>2.7%
Reproduction NOEC:
2.7%
Overall Method:
Homoscedastic t
Normal Distribution:
Yes
Method:
Shapiro-Wilk's
Statistic:
0.973
Critical Value:
0,984
Equal Variances:
Yes
Method:
F-Test
Statistic:
3.205
Critical Value:
5.320
Non -Parametric Analysis (ifapplicable)
Method:
Effluent %
Rank Sum
Critical Sun:
2.7%

Am�
RUDENR
North Carolina Department of Environment and Natural Resources
Beverly Eaves Perdue
Governor
Division of Water Quality
Coleen H. Sullins
July 10, 2009
Mr. Gregory Phillips
Duke Power River Bend Steam Station
175 River Bend Road
Mount Holly, North Carolina 28120
Dear Mr. Phillips:
Director
Subject: Toxicity Test Results
Duke River Bend Steam Station
NPDES Permit No. NC0004961
Gaston County, NC
Dee Freeman
Secretary
Enclosed is a copy of the toxicity test results relative to the inspection conducted at the subject facility
on June 23 and June 26 by Donna Hood and John Lesley of this Office. Effluent samples were collected at
outfall 002 on June 23 and 26, 2009 by Division staff for use in a chronic pass/fail toxicity test. The test
utilizing these samples resulted in a pass.
The report should be self-explanatory; however, should you have any questions concerning this report,
please do not hesitate to contact Mr. Lesley or me at [phone].
Sincerely,
Zert B. Krebs
L� Surface Water Protection
Regional Supervisor
Enclosure
cc: Central Files
Susan Meadows, Aquatic Toxicology Unit
Mooresville Regional office
Location: 610 East Center Ave., Suite 301 Mooresville. NC 28115
Phone: [phone] \ Fax: [phone] \ Customer Service: 1-877-623-6748
Internet: w%vw.ncwaterquality.org
An Equal Opportunity \ Affirmative Action Employer - 50% Recycled/10% Post Consumer paper
NorthCarolina
naturally

-)Z_
Division of Water Qualitv
July 6, 2009
MEMORANDUM
To: Rob Krebs
Regional Supervisor, Surface Water Protection, MRO
Through: Cindy A. Moore C*
Supervisor, Aquatic Toxicology Unit
From: Carol Hollenkamp (/
Quality Assurance Officer, Aquatic Toxicology Unit
Subject: Whole effluent toxicity test results
Duke Power -River Bend 002
NPDES Permit # NC0004961/002
Gaston County
The aquatic toxicity test using grab samples of effluent discharged from Duke Power- River Bend 002 has
been completed. Duke Power- River Bend 002 has an effluent discharge permitted that is not limited
entering the Catawba River (7Q10 of 80.0 CFS). Whole effluent samples were collected as grab samples on
June 23 by Donna Hood and on June 26 by John Lesley and a facility representative for use in a chronic
Ceriodaphnia dubia pass/fail toxicity test. The test using these samples resulted in a pass. Toxicity test
information follows.
Test Type
Test Concentrations
Test Result
Control Survival
Control Mean Reproduction
Test Treatment Survival
Treatment Mean Reproduction
First Sample pH
First Sample Conductivity
First Sample Total Residual Chlorine
. Second Sample pH
Second Sample Conductivity
Second Sample Total Residual Chlorine
3-Brood Ceriodaphnia dubia pass/fail
10.0% sample
Pass
100%
18.9 neonates
92%
19.8 neonates
8.38 SU
132.9 micromhos/cm
<0.1 mg/L
7.41 SU
138 micromhos/cm
<0.1 mg/L
Test results for the above samples indicate that the effluent would not be predicted to have water quality
impacts on receiving water. Please contact us if further effluent toxicity monitoring is desired. We may be
reached at [phone].
Basin: CTB 33
cc: Central Files
John Lesley, MRO
Donna Hood, MRO
Aquatic Toxicology Unit E1701•01:1aental Sciences Section

W A1FR
Michael F. Easley. Goverodr
O�
William G. Ross Jr., Secrete
North Carolina Department Environment and Natural Resources
of
7
Coleen Sullins. Director
Division of Water Quality
March 3, 2008
Mr. Gregory Phillips
Duke Power River Bend Steam Station
175 River Bend Road
Mount Holly, North Carolina 28120
Subject: Toxicity Test Results
Duke River Bend Steam Station
NPDES Permit No. NC0004961
Gaston County, NC
Dear Mr. Phillips:
Enclosed is a copy of the toxicity test results relative to the inspection conducted at the
subject facility on February 19, 2008 by John Lesley of this Office. Effluent samples were
collected at outfall 002 on February 19 and 22, 2008 by Division staff for use in a chronic
pass/fail toxicity test. The test utilizing these samples resulted in a pass.
The report should be self-explanatory; however, should you have any questions
concerning this report, please do not hesitate to contact Mr. Lesley or me at [phone].
Sincerely,
obert B. Krebs
Surface Water Protection
Regional Supervisor
Enclosure
cc: Central Files
Susan Meadows, Aquatic Toxicology Unit
Gaston County Health Department
0.
NprNaCarolina
turally
N. C. Division of Water Quality, Mooresville Regional Office, 610 E. Center Ave. Suite 301, Mooresville NC 29115 [phone] Customer Service
1-877-623-6748

Duke Power River Bend Steam Station
NPDES Permit No. NC0004961
Page 2
Whole effluent samples were collected at outfall 002 on February 19 and 22, 2008 by John
Lesley for use in a chronic Ceriodaphnia dubia pass/fail tonicity test. Duke Power River Bend
Steam Station out fall 002 has an effluent discharge entering the Catawba River (7Q10 of 80.0
CFS). The test using these samples resulted in a pass. Toxicity test information follows:
Test Type
Test Concentration
Test Result
Control Survival
Control Mean Reproduction
Test Treatment Survival
Treatment Mean Reproduction
First Sample pH
First Sample Conductivity
First Sample Total Residual Chlorine
Second Sample pH
Second Sample Conductivity
Second Sample Total Residual Chlorine
3-Brood Ceriodaphnia dubia pass/fail
10% sample
Pass
100%
29.3 neonates
100%
32.3 neonates
8.36 SU
156 micromhos/cm
<0.0052 mg/l
7.34 SU
156 micromhos/cm
<0.0052 mg/l
Test results for these samples indicate that the effluent would not be predicted to have
water quality impacts on the receiving stream.

Michael F. Easley, Gover r b—
William G. Ross Jr., Secretary
North Carolina Department of Environment and Natural Resources
August 2, 2006
Mr. Gregory Phillips
Duke Power River Bend Steam Station
175 River Bend Road
Mt. Holly, NC 28120
Subject: Toxicity Test Results
Duke Power River Bend Steam Station
NPDES Permit No. NC0004961
Gaston County, NC
Dear Mr. Phillips:
Alan W. Klimek, P.E. Director
Division of Water Quality
Enclosed is a copy of the toxicity test results relative to the inspection conducted at the
subject facility on July 18, 2006 by John Lesley of this Office. Effluent Samples were collected on
July 18 and 21, 2006 by Division staff for use in a chronic Ceriodaphnia dubia pass/fail toxicity
test. The test utilizing these samples resulted in a pass.
The report should be self-explanatory; however, should you have any questions concerning
this report, please do not hesitate to contact Mr. Lesley or me at [phone].
Sincerely,
D. Rex Gleason, P.E.
Surface Water Protection Regional Supervisor
Enclosure
cc: Central Files
Kevin Bowden, Aquatic Toxicology Unit
Gaston County Health Department
NCDENR
One
NCarolina
NWAMu!!y
Mooresville Regional Office Division of Water Quality Phone [phone] Customer Service
Internet: www.ncwaterquality org 610 East Center Ave, Suite 301 Mooresville, NC 28115 Fax [phone]
An Equal Opportunity/Affirmative Action Employer— 50%, Recycled/10% Post Consumer Paper

Duke Power River Bend Steam Station - outfall 002
NPDES Permit No. NC0004961
Page 2
Whole effluent samples were collected on July 18 and 21, 2006 by John Lesley for use in a
chronic Ceriodaphnia dubia pass/fail toxicity test. Duke Power River Bend Steam Station - 002
has an effluent discharge entering the Catawba River (7Q10 of 80.0 CFS). The test using these
samples resulted in a pass. Toxicity test information follows:
Test Type
Test Concentration
Test Result
Control Survival
Control Mean Reproduction
Test Treatment Survival
Treatment Mean Reproduction
First Sample pH
First Sample Conductivity
First Sample Total Residual Chlorine
Second Sample pH
Second Sample Conductivity
Second Sample Total Residual Chlorine
3-Brood Ceriodaphnia dubia pass/fail
10% sample
Pass
100%
20.5 neonates
100%
21.6 neonates
7.32 SU
195 micromhos/cm
<0.0052 mg/1
7.14 SU
194 micromhos/cm
<0.0052 mg/1
Test results for these samples indicate that the effluent would not be predicted to have
water quality impacts on the receiving stream.

Division of Water Quality
July 27, 2006
MEMORANDUM
To: Rex Gleason
Regional Supervisor, Surface Water Protection, MRO
From: Sandy Mort ApA-
Quality Assurance Officer, Aquatic Toxicology Unit
Subject: Whole effluent toxicity test results
Duke Power -River Bend 002
NPDES Permit # NC0004961/001
Gaston County
_ - jhr 1. O '
Akt) 14ATURAL RESOURCES
$WRESVILLE REGIONAL OFFICE
R F. C7 :' W
JUL. 3 1 2006
VIER QUAUTY SECTION
The aquatic toxicity test using grab samples of effluent discharged from Duke Power -River
Bend 002 has been completed. Duke Power -River Bend 002 has an effluent discharge
(permitted flow = "NA") entering Catawba River (7Q10 of 80.0 CFS). Whole effluent
samples were collected on July 18 and 21, 2006 by John Lesley for use in a chronic
Ceriodaphnia dubia pass/fail toxicity test. The test using these samples resulted in a pass.
Toxicity test information follows.
Test Type 3-Brood Ceriodaphnia dubia pass/fail
Test Concentrations 10% sample
Test Result Pass
Control Survival 100%
Control Mean Reproduction 20.5 neonates
Test Treatment Survival 100%
Treatment Mean Reproduction 21.6 neonates
First Sample pH 7.32 SU
First Sample Conductivity 195 micromhos/cm
First Sample Total Residual Chlorine <0.0052 mg/L
Second Sample pH 7.14 SU
Second Sample Conductivity 194 micromhos/cm
Second Sample Total Residual Chlorine <0.0052 mg/L
Test results for these samples indicate that the effluent would not be predicted to have water
quality impacts on receiving water. Please contact us if further effluent toxicity monitoring is
desired. We may be reached at [phone].
Basin: CTB33
cc: Central Files
John Lesley, MRO

0� WA TFR Michael F. Easley, Gor-.-I
William G. Ross Jr., Secretary
North Carolina Department of Environment and Natural Resources
co 7
> Alan W. Klimek, P. E., Director
Division of Water Quality
Coleen H. Sullins, Deputy Director
Division of Water Quality
September 3, 2003
Mr. Michael Ruhe
Manager, Water Protection
Duke Energy Corporation
Post Office Box 1006 (EC 11E)
Subject: Toxicity Test Results
Duke Power River Bend Steam Station
NPDES Permit No. NC0004961
Gaston County, NC
Dear Mr. Ruhe:
Enclosed is a copy of the toxicity test results relative to the inspection conducted at the
subject facility on August 22, 2003 by John Lesley of this Office. Effluent Samples were collected
at outfall 002 on August 19 and 22, 2003 by Division staff for use in a chronic toxicity test. The
test utilizing these samples resulted in a pass.
The report should be self-explanatory; however, should you have any questions concerning
this report, please do not hesitate to contact Mr. Lesley or me at [phone].
Sincerely,
n
D. Rex Gleason, P.E.
Water Quality Regional Supervisor
Enclosure
cc: Central Files
Aquatic Toxicology Unit
Gaston County Health Department
NCDEh'R
Mooresville Regional Office, 919 North Main Street, Mooresville, North Carolina 28115
Phone [phone] Customer Service
Fax [phone]

Duke Power River Bend Steam Station
NPDES Permit No. NC0004961
Page 2
Whole effluent samples were collected on August 19 and 22, 2003 by John Lesley of this
Office for use in a chronic Ceriodaphnia dubia pass/fail toxicity test. Duke Power River Bend
Steam Station has an effluent discharge (outfall 002) permitted at 5.98 MGD entering the Catawba
River (7Q10 of 80.0 CFS). The test using these samples resulted in a pass. Toxicity test
information follows:
Test Type
Test Concentration
Test Result
Control Survival
Control Mean Reproduction
Test Treatment Survival
Treatment Mean Reproduction
First Sample pH
First Sample Conductivity
First Sample Total Residual Chlorine
Second Sample pH
Second Sample Conductivity
Second Sample Total Residual Chlorine
3-Brood Ceriodaphnia dubia pass/fail
10.0% sample
Pass
100%
27.0 neonates
100%
28.2 neonates
6.96 SU
227 micromhos/cm
<0.0052 mg/l
6.82 SU
229 micromhos/cm
<0.0052 mg/l
Test results for these samples indicate that the effluent would not be predicted to have
water quality impacts on the receiving stream.

Division of Water Quality
- Aquatic Toxicology Unit
August 28, 2003
MEMORANDUM
To: Rex Gleason
Water Quality Supervisor, MRO
Through: Matt Matthews (Y`M
Supervisor, Aquatic Toxicology Unit
From: Sandy Mort A
Quality Assurance Officer, Aquatic Toxicology Unit
Subject: Whole effluent toxicity test results
Duke Power -River Bend 002
NPDES Permit # NC0004961/002
Gaston County
NC UEPT. OF ENVIRONMEi:T
AND NATURAL RESOURCES
t1OORES`t., ' �l,OFFICE
SEP 0 2 2003
The aquatic toxicity test using grab samples of effluent discharged from Duke Power -River Bend 002
has been completed. Duke Power -River Bend 002 has an effluent discharge permitted at 5.98 million
gallons per day (MGD) entering Catawba River (7Q10 of 80.0 CFS). Whole effluent samples were
collected on August 19 and 22, 2003 by John Lesley for use in a chronic Ceriodaphnia dubia
pass/fail toxicity test. The test using these samples resulted in a pass. Toxicity test information
follows.
Test Type
Test Concentrations
Test Result
Control Survival
Control Mean Reproduction
Test Treatment Survival
Treatment Mean Reproduction
First Sample pH
First Sample Conductivity
First Sample Total Residual Chlorine
Second Sample pH
Second Sample Conductivity
Second Sample Total Residual Chlorine
3-Brood Ceriodaphnia dubia pass/fail
10.0% sample
Pass
100%
27.0 neonates
100%
28.2 neonates
6.96 SU
227 micromhos/cm
<0.0052 mg/L
6.82 SU
229 micromhos/cm
<0.0052 mg/L
Test results for these samples indicate that the effluent would not be predicted to have water quality
impacts on receiving water. Please contact us if further effluent toxicity monitoring is desired. We
may be reached at [phone].
Basin: CTB33
cc: Central Files
Environmental Sciences Branch Water Quality Section

Division of Water Quality
July 17, 2001
MEMORANDUM
To: Rex Gleason
Water Quality Supervisor, MRO
Through: Matt Matthews 10VN
Supervisor, Aquatic Toxicology Unit
From: Sandy Mort
Quality Assurance Officer, Aquatic Toxicology Unit
Subject: Whole effluent toxicity test results
Duke Power -River Bend 002
NPDES Permit # NC0004961/002
Gaston County
41C DEP C cIF :-KNIROM"'T
AM NATURAL 'RESh"D!'R(.'E$
JUL 1 9 2001
WATER QUALL r f Scc 1 ION
The aquatic toxicity test using grab samples of effluent discharged from Duke Power -River Bend
002 has been completed. Duke Power -River Bend 002 has an effluent discharge permitted at
5.98 million gallons per day (MGD) entering Catawba River (7Q10 of 80.0 CFS). Whole
effluent samples were collected on July 10 and 13 by John Lesley for use in a chronic
Ceriodaphnia dubia pass/fail toxicity test. The test using these samples resulted in a pass.
Toxicity test information follows.
Test Type 3-Brood Ceriodaphnia dubia pass/fail
Test Concentrations 10% sample
Test Result
Pass
Control Survival
100%
Central Mean Rcproductio..
25.4 neonates
Test Treatment Survival
100%
Treatment Mean Reproduction
28.1 neonates
First Sample pH
7.70 SU
First Sample Conductivity
176 micromhos/cm
First Sample Total Residual Chlorine
<0.1 mg/1
Second Sample pH
8.27 SU
Second Sample Conductivity
171 micromhos/cm
Second Sample Total Residual Chlorine
<0.1 mg/1
Test results for these samples indicate that the effluent would not be predicted to have water
quality impacts on receiving water. Please contact us if further effluent toxicity monitoring is
desired. We may be reached at [phone].
Basin: CTB33
cc: Central Files
Environmental Sciences Branch Water Quality Section