NC0031836_Regional Office Historical File Pre 2018

AIM
i
January 7, 2009
NCDENR — Division of Water Quality
Raleigh Central Office
1617 Mail Service Center
Raleigh, NC 27699-1617
CC: Files
Re: Toxicity Data
Fourth Creek Wastewater Treatment Plant
NPDES Permit No. NCO031836
Dear Mr. Adu-Poko:
Enclosed you will find the remaining effluent toxicity data regarding the 4th Creek WWTP permit
renewal application. I made mention of this data in the original cover letter for this renewal application.
This will complete all of the data that your office requested.
Should you require more information, or if further action is required, please contact me at 878-3438
Si 4rly,
Andy Smith
Plant Supervisor, Fourth Creek WWTP
JA N 2 0 2009
DUAU- 4rfac'r:rt�;cn

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 12/18/08
Facility: STATESVILLE-4TH CREEK 36% NPDES#: N00031836 Pipe#: 01 County: IREDELL
Labq�aty P�zformin,Test: MERITECH, INC
, i /4` /l 1Comments: Dilution Water Batch
X
#709 & 710 Used
Signature of Laboratory Supervisor I * PASSED: -2.61% Reduction **
Work Order: Environmental Sciences Branch
MAIL ORIGINAL TO: Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
I-- - - -- - - ---- -
c
Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t = -0.654
Tabular t = 2.508
'ONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Reduction = -2.61
# Young Produced 24 28 27 25 26 26 26 29 27 20 25 24
Adult (L) ive (D) ead L L L L L L L L L L L L
'ffluent %• 36%
% Mortality
Avg.Reprod.
0.00
25.58
Control
Control
0.00
26.25
Treatment 2
Treatment 2
'REATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Control CV
9.046% PASS FAIL
# Young Produced 25 30 23 27 28 26 27 29 29 26 24 21 % control orgs X
producing 3rd
brood Check One
Adult (L) ive (D) ead L L L L L L L L L L L L 100%
1st sample 1st sample 2nd sample Complete This For Either Test
pH Test Start Date: 12/10/08
Control 7.90 7.64 7.90 7.90 7.92 7.82 Collection (Start) Date
Sample 1: 12/08/08 Sample 2: 12/11/08
Treatment 2 7.56 7.87 7.91 7.86 7.57 7.77 Sample Type/Duration 2nd
lst P/F
s s s Grab Comp. Duration D
t e t e t e I S S
a n a n a n Sample 1 X 24.0 hrs L A A
r d r d r d U M M
t t t Sample 2 X 24.0 hrs T P P
1st sample 1st sample 2nd sample
D.O. Hardness (mg/1) 44
.......... ..........
Control 7.60 7.55 7.84 7.60 7.55 7.45
Spec. Cond.(µmhos) 178 389 330
Treatment 2 7.88 7.61 7.79 7.52 7.54 7.50
Chlorine (mg/1) ,,,,,,,, <0.1 <0.1
LC50/Acute Toxicity Test Sample temp. at receipt(°C) ,,,,,,,, 2.0 1.9
(Mortality expressed as %, combining replicates)
Note: Please
Concentration Complete This
Section Also
Mortality
start/end Mren
LC50 = % Method of Determination
95% Con ire Limits Moving Average Probit _
% -- % Spearman Karber _ Other
Control
a]
High
---
pH
Organism Tested: Ceriodaphnia dubia Duration(hrs):
Copied from DWQ form AT-1 (3/87) rev. 11/95 (DUBIA ver. 4.41)
F •

Meritech, Inc.
c� l j Mini Chronic Pass/Fail T st: Ceriodaphnia dubia Incubator #:
Client: \'cL� U t II e q Geek Pipe #: d 1 JCounty: — Date Start: Z- iD D s, Date End: 12- 7/0
NPDES #. NC Cxo 3 t S'3& Date / Time of Culture Transfer: qAfi Time Start: hm Time End: 'C?C' Ax\
Dilution Water: Lake Reidsville Date / Time Neonates born: 1st Renewal Date: 1-2 j zlor Time: J.S
Test Organism Source: Tray #__�f_ Age of neonates at Test Start: , hours 2nd Renewal Date: / V15- or Time:
Stirred / Aerated for D.O.: Y /® Randomized:Y / N Culture Tray Temp: ✓1 °C Analyst(s): Reviewed by: '—
Dav #2 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced
b
U
O
I
0
U
0
I
C�
0
0
I
Adults Live / Dead
C_
L
-
L
L.
l__
L_
l_
L
L,
L.
-
Dav #5 1 2 3. 4. 5 6 7 8 9 10 11 12
# Young Produced
Adults Live / Dead
Dav #7 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced
J
f
;_
%
Adults Live /Dead
L
L-
L
L
L
(
L
L
t-
L_
Total Produced Lkq ke , n(U -, x) ) ,
Percent of Control producing third brood: (h %
Test Sample Organism Reproduction
Effluent %: -3
Dav #2 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced
U
O
C
C)
C
U
C)
eD
G
Ca
Adults Live / Dead
r1
tr
L
C,
L
tC--
Dav #5 1 2, 3 4 5 6 7 8 9 10 11 12
# Young Produced
Adults Live /.Dead
L
L-
(_
L
L
j_
L
(_
Dav #7 1 2 3 4 5 6 7 8 9 10 11 12
# Youn Produced
(�
Adults Live / Dead
L
L
(,
L
L
L
L
L
L
L
Total Produced �� .'�'� �20 2 j ��,d I
Comments:
Sample 1: 1 zdglUSf Sample 2.-
Sample Information
100% pH
G / C?
Duration
Sample 1
7.16
'2- hours
Sample 2
-7. l7
'Z hours
Batch #
/)6_
(l
�io
Sample
Sample
_�
Transfer Day
0
2
5
Hardness
(mg/L)
`-�a
��11
q V
Spec. Cond.
(umhos/cm)
Chlorine
(mg/L)
Receipt Sample
Temp (°C)
2.0
J9
1``1
nH 1st Samole
Control
7.qo
Sample
7`.,V
/
D.O. 1St Samnla
Control
7,60='
S
Sample
7A;
i,�^
Temp. 1st Samole
Control
10
TO
Sample
2o
14
Transferred by: Fed by:
Day 0 ✓h(1- GU
Day 1
Day 2 'L %y'
Day 3
Day 4 r�
Day 5 >~bfh— lilt.
Day 6
Terminated by:
1st Sample 2nd Sample
7,y�)
1st Sample �,I' 2nd Sample
7.
1 st Sample 2nd Sample
11V� All z q

MERITECH, INC. MeritechSample ID#:
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txmrtech(a)bellsouth.net Web Site: www.meritech-labs.com
1 Y)V
CLIENT INFORMATION
Client:--
6-75 � .>, , 1 t
Contact Person:
NPDES#: NC O, �) -3%,?36
Address:,!
Phone:i 4
City:
J Cf'�5
~�a
Pipe #: 01
County:
_z7/Z&�
State:
/ VCR Zip:
25
SAMPLE INFORMATION
Sample Site:
Sample Type:
❑ Grab
Composite
# of containers:
Z
Sampling Time:
Start Date:
U
U Start Time:
AM PM
End Date:
�. Q a� End Time:
S AM PM
—► CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
***SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED AND COVERED WITH ICE***
Collector's Name: Print:
Signature:
TOXICITY TEST INFORMATION
Test Required:
K Chronic (7 days)
Test Organism: )) Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours)
X Pimephales prorrielas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
iwc:-36 %
Test Concentrations (if multiple dilutions):
Comments:
5 j
SHIPPING INFORMATION
Relinquished by:
Date: Z l% d Time: D Z
AM PM
Received by:
_ -
Date: / 2 � � - � Time: �
� PM
Relinquished b
q Y
— — .0
Date: f Z 0 Time: j
AM.: PM
Received by:
Date: Time:
AM PM
Sample Temperature (°C):
Method of Shipment:
❑ UPS ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: G f
Received b Date: �� / Time: ) S AM
Sample Temperatures (°C): �� /� ,0 / / Sample Condition: 1 cc(
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. Meritech Sample ID #:
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
�! Email: txmrtechCoDbellsouth.net Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: S� TlLfiC% PO#:
Contact Person: ���j`/fi� Z.� � NPDES#:_NC OD31F9L
Address: 3 Phone:—-3y3g'
City: fs Ir�i Pipe #:
County: State: )VC— Zip: z j
SAMPLE INFORMATION
Sample Site:
Sample Type: ❑ Grab Composite # of containers:
Sampling Time: StartDate://�� Start Time: IZ�Vp 0 PM
End Date: ! �14: End Time: Qa AM PM CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AND COVERED WITH ICE ***
Collector's Name: Print: o1j�� tnw­re+f Signature:
TOXICITY TEST INFORMATION
Test Required: , Chronic (7 days) Test Organism: V Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours) X Pimephales pronielas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
' Iwc.: % Test Concentrations (if multiple dilutions):
j�
Comments: f j/ 'I!% z5
SHIPPING INFORMATION
Relinquished by: tom , _ Date: 1 Time: /2-4 S�
Received by: f" Date: A2— —/ e7i —� Time: 45
Relinquished by:
Received by:
Date:
Date:
Sample Temperature (°C):
Time:
Time:
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
AM PM
AM PM
AM PM
AM PM
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
Received Date: Ar, C Time: � AM M
Sample Temperatures C): �� / / / Sample Condition: C
WHITE = Laboratory copy YELLOW = Client copy

Effluent Toxicity Statistical Results
- Chronic
Pass/Fail Date: 12/18/08
Facility: STATESVILLE-4TH CREEK 36%
NPDES#:
NCO031836 Pipe#: 01 County:IREDELL
Laboratory Performing Test: MERITECH,
INC
Reduction: -2.61%
CONTROL
36% Effluent
# Replicates
12
12
Female Live
12
12
Adult Male
0
0
Adult Dead
0
0
Adult Mortality
0.00%
0.00%
# Neonates
307
315
Mean # Neonates
25.583
26.250
Standard Deviation
2.314
2.667
Coefficient of Variation
9.046%
Fisher's Exact Test
A = 12 B = 12 a = 12 b = 12
a/A = 1.00 b/B = 1.00
Success is: survival
Critical b value = 8
12 > 8
The test concludes that the proportion of survival is not
significantly different for the control and the effluent groups.
Test Passes!
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA
ORDERED OBSERVATIONS
i Group Neonates Centered i Group Neonates Centered
1
C
20
-5.5833
13
C
26
0.4167
2
E
21
-5.2500
14
C
26
0.4167
3
E
23
-3.2500
15
E
27
0.7500
4
E
24
-2.2500
16
E
27
0.7500
5
C
24
-1.5833
17
C
27
1.4167
6
C
24
-1.5833
18
C
27
1.4167
7
E
25
-1.2500
19
E
28
1.7500
8
C
25
-0.5833
20
C
28
2.4167
9
C
25
-0.5833
21
E
29
2.7500
10
E
26
-0.2500
22
E
29
2.7500
11
E
26
-0.2500
23
C
29
3.4167
12
C
26
0.4167
24
E
30
3.7500

SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.)
i
x(n-i-1)
COEFFICIENTS AND
x(i)
DIFFERENCES
a(i)
x(n-i-1) - x(i)
1
3.7500
-5.5833
0.4493
9.3333
2
3.4167
-5.2500
0.3098
8.6667
3
2.7500
-3.2500
0.2554
6.0000
4
2.7500
-2.2500
0.2145
5.0000
5
2.4167
-1.5833
0.1807
4.0000
6
1.7500
-1.5833
0.1512
3.3333
7
1.4167
-1.2500
0.1245
2.6667
8
1.4167
-0.5833
0.0997
2.0000
9
0.7500
-0.5833
0.0764
1.3333
10
0.7500
-0.2500
0.0539
1.0000
11
0.4167
-0.2500
0.0321
0.6667
12
0.4167
0.4167
0.0107
0.0000
1
W = X 130.385B
137.1667
Calculated W = 0.951 Critical W = 0.884
0.951 Z 0.884
The reproduction data is normally distributed
evaluated at a 99% confidence interval.
Test Passes!
F test for Homogeneity of Variance
Effluent variance 7.1136
F = _ = 1.33
Control variance 5.3561
Numerator degrees of freedom: 11 Denominator degrees of freedom: 11
Critical F = 5.32
1.33 5 5.32 =► The Test PASSES, the variances of the two groups
are significantly the same, homogeneous.

EQUAL VARIANCE t TEST
25.6 - 26.3
t = = -0.654
1.019
Degrees of freedom = 22
Critical t = 2.508
-0.654 < 2.508
Test passed. There is not a significant difference in reproduction
between the Control and the effluent evaluated at a 99% confidence interval.
Chronic Test PASSES

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:12/22/2008
Facility: Statesville - Fourth Creek
t
A
rat Meri ch, Inc.
x
Signature of O erator in Responsit
Signature of Laboratory Supervisor
Test Initiation Date/Time
% Eff. Repl.
Control Surviving #
Original #
Wt/original (mg)
0 Surviving #
Original #
Wt/original (mg)
18 Surviving #
Original #
Wtoriginal (mg)
36 Surviving #
Original #
Wt/original (mg)
55 Surviving #
Original #
Wt/original (mg)
73 Surviving #
Original #
Wt/original (mg)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp. at Receipt (°C)
Dilution H2O Batch #
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
NPDES # NC00 31836
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
12/9/2008
1
3:15 PM
2 3 4
Pipe #: 001 County: Iredell
Comments
Avg WUSurv. Control 0.635 Test Organisms
Cultured In -House
% Survival 100.0 Outside Supplier
Avg Wt (mg) 0.635 Hatch Date: 12/8/08
10
10
10
10
10
10
10
10
0.670
0.590
0.649
0.630
10
10
10
10
10
10
10
10
0.568
0.527
0.605
0.657
10
10
10
9
10
10
10
10
0.641
0.645
0.657
0.553
9
9
10
10
10
10
10
10
0.669
0.653
0.699
0.606
10
10
10
10
10
10
10
10
0.632
0.620
0.656
0.574
Day
% Survival 100.0 Hatch Time: 3:00 pm CT
Avg Wt (mg) 0.598
% Survival 100.0
Avg Wt (mg) 0.589
% Survival 97.5
Avg Wt (mg) 0.624
% Survival 95.0
Avg Wt (m
g)F. 0.657
% Survival 100.0
Avg Wt (m
g).F 0.621
0 1 2 3 4 5 6
7.92
/ 7.47
8.03
/ 7.72
7.86
/ 7.75
8.01
/ 7.79
8.01
/ 7.62
7.93
/ 7.56
7.94
17.62
7.59
/ 6.50
7.59
/ 7.64
7.97
/ 7.42
7.81
/ 7.75
8.03
/ 7.40
7.93
/ 7.38
7.83
/ 7.28
25.2
/ 24.9
25.0
/ 24.8
25.0
124.8
24.9
/ 25.2
25.0
124.8
25.0
/ 24.9
24.9
/ 24.9
0 1 2 3 4 5 6
7.26
/ 7.48
7.42
/ 7.63
7.60
17.68
7.72
/ 7.75
7.41
/ 7.32
7.23
17.48
7.18
/ 7.55
8.08
/ 6.27
7.67
/ 7.46
7.93
/ 7.09
7.70
/ 7.75
7.35
/ 6.48
7.45
/ 6.48
6.88
/ 6.73
25.0
/ 24.9
24.8
/ 24.8
25.2
/ 24.8
25.1
1 25.2
25.0
/ 24.8
25.0
/ 24.9
24.9
/ 24.9
1 2 3
12/8/2008
12/9/2008
12/11 /2008
24.0
24.0
24.0
42
40
50
56
1 52
1 62
389
405
327
<0.1
<0.1
<0.1
2.0
1.9
1.9
314 315 316 317
48 44 44 40
53 54 56 57
201 208 208 223
Survival
Growth
Normal
Hom. Var.
NOEC
73
73
LOEC
>73
>73
ChV
>73
>73
Method
Steel's
Dunnet's
Overall Result
ChV >73
Stats
Survival
Growth
Conc.
Critical
Calculated
Critical Calculated
9
10
18
2.41 1.3056
18
10
18
2.41 1.6165
36
10
16
2.41 0.3819
55
10
14
2.41-0.7816
73
10
18
2.41 0.5063

Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Client: 5tatesvi I le - 4th Creek # of Organisms per Chamber: 10
NPDES #: NC 0031836
Initiated by:
Date/Time Fed: 12/9/08 `�^✓
Date/Time Born: 12/8/08 3:00 PM CT
Transferred by:
Test Vessel Size: 400 ml
Test Solution Volume: 250 ml
Temp. of Stock: °C
# of Reps: 4
Start Date: 12/9/08 Time: f 5
End Date: 12/16/08 Time:
Randomization: Yes No
Incubator #: 2
Fed by:
Date Time Initials Date Time 1 Initials Time 2 Initials Time 3 Initial
Day 1 12/10 1Z 4001411 RA, Day 0 12/9 / ' ,WM M-L.
Day 2 12/11 ' -,a' Day 1 12/10 {'h !- J? ; t(5," Iutil�
Day 3 12/12 �.1Cb rtl �/ Day 2 12/11
/ r
Day 4 12/13 ��� Day 3 12/12 Am
I� l ZW 14,1 `%.�
12/14 '�/� 12 1 ����^^'' *
Day 5 CGS /'L Day 4 / 3 `y/CGt.� �l j�
Day 6 12/15 y%/ Day 5 12/14
Day 6 12/15
Test Termination Data: , /' /O/AL _
Initials/Signature: AIL -
End Date: 12/16/08
End Time: / 0 311au
= Weekend, only 2 feedings needed

It/1 L TECH, /NC_
ENVIRONMENTAL LABORATORIES
A Uivisioi> c: LVn, ter Ti>yy a�d C-vntro/s, /nc
Chemical and Physical Determinations
Client: 5-}bAa5\A1L - Greek NPDES#: Nc tx,3i f3--,,5& StartDate: 12-c O Time: 31S- ,�--
Test Organism: Pimephaies promelas Analyst(s): Z 1-&Z- End Date: tz f(v 0 (? Time:
Day
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Batch #
.a (
3
-�-�
ID
---��
j
pH Initial
7,04 Z
5,0 3
7a � i
,Q (
L) i
'j q3
Final-?,
q?
2. 7
1.7
6e2-�
7, (0 Z.
D.O. Initial
7,�
1917
7..
Final
.5-0
7.tq q
7. q Z-
Temp. Initial
Z. , Z
ZJ
�r C
- 4, ��
?S-1 0
Z
Final
2-L4 cl
2q:b
.c
Conductivity Initial
ICj(o
?�
2W
1 3
Final
"Z,
Residual Chlorine
Z-0 ,
Hardness
q 1�
—�
Alkalinity
Dav
Concentration: /
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH Initial
-7, �;
7U
G
7- 93
5
1,15
7�
Final
?. --5Z
% 7
�`
7 7 k
' . 6q
7•
6o
D.O. Initial
7 (U0
i
`sl
7 M
7
%f, `1 I
-2,P O
Final
S
b
7.
. 73
`l, 23
-.17
i
Temp. Initial
2 S Z
-2 -0
Final
2-9 .q
2
q, s/
y
Z
Conductivity Initial
2-j
2 a
2-26
2451
Final
Residual Chlorine
Dav
Concentration:
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH Initial
, Q
%
7 -7'i
�-�
.`7)
�lo
Final
, f.�
q
777
, � '
, Z
, 6�
D.O. Initial
7
��
�.g/
, ;`
7 & ,
Final
L
7q%
7..7q
72-g
7•-3
Temp. Initial
S. Z
S ., U
2 ;,0
��
Final
29
24- Q,
? t� , S�
v� 2--
?
-I,
Conductivity Initial
214(}
7:�-4 S
213S
g9s-
a
Final0
Residual Chlorine
-Zfl/ I
/-6-
i
�� . i
/ �',I
L�`,
�u,

MER/TECH, //V C_
IENViRONMENTL�L'LABORATO RIE S"`
Chemical and Physical Determinations
Client: J`'+Aeaddle - y�Gfeek NPDES#: Auer prJ :2,lg3& StartDate: 12-q�bff
Test Organism: Pimepha►es promeias Analyst(s): 0L /0—End Date:
Dav
Page 2 of 2
Time:
Time:c..�
Concentration: 3(p %,
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH Initial
7, '
7,&
% S
q. q 2-,,
Final
�7, � S
7• (0
�. � 47
D.O. Initial
, 7
7 L_ 0'
7.
s
,7
Final
3
�,
�,$-
(.'�
-2- 0 3
Temp. Initial
Z�Z
Z`{�q
�5,
=i '%
S ��
S,©
ZV.9
Final
2� ,Z
Z'i
Lei
Conductivity Initial
,2
7 ?
Z'i
.L
2�5
�l
Final
J/,S—t
(�
$
Residual Chlorine
z�,C`
Day
Concentration: 6157'/,
0-1
1-2
2-3
34
4-5
5-6
6-7
Remarks
pH Initial
7
`7
- ;
-1,41
7, 3Z
Final
, �{
?.?
9
--2,
D.O. Initial
?.Cf f
Z
7 .
7. ? cl
7
Final
�.3C�;
S70
,2
2, 7 �
f! . J
Temp. Initial
��
Z-
2j,L
;� , i
2S''_
' j,0
z ,
Final
Zq l
2q.
.,)
Conductivity Initial
:= j �=
-��
Final
- ?) f
Residual Chlorine
l
c.
�a,�
t-(,
zz • !
/-C', ,/
l e'
Da
Concentration: 7.3 Z
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH Initial
L&
7, q 2
7,
7,-2
`j,
'? -16
Final
�, �G•'3
?- %��
7
j)
5
D.O. Initia
I X,C)
,7
7,
7
1,
, e
Final
2-'7
1 � b-
7 '7.S
, 4
73
Temp. Initial
Z, , (�
`-j j
. Z
? I
a 5 •cal
25, G
IV,
Final
2
2.4•a
Conductivity Initial
�}
7✓
050
3'
�}
i�5
(;
Final77
i.J
340,
3
10
Residual Chlorine
Day
Concentration: 100%
0-1
1-2
2-3 3-4
4-5
5-6
6-7
Remarks
pH
.1A
7 .
Conductivity
141
Residual Chlorine
< Gl,
L
Hardness
441
q0
SO
Alkalinity
(U
Z

MERITECH, INC.
Mortality Data: Chronic Fathead Test
qk
Client: ) oAes i lc' ' `t Cie,,--: Start Date: + `%lGb'Start Time: 5
Z ��
End Date: G End Time: ,,-� ��-
Concentration
�p
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
0
-
"�
0
0
�
0
0
�-
0
Rep #2
V
d
Rep #3
Rep #4
U
`�
t
U
Ll
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Concentration
P
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
V
C>
Rep #2
v
r1
0
V
y
0
Rep #3
Rep #4
U
U
l
�_
Concentration
($
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
i]
0
V
Rep #1
Rep #2
V
(�
v
0
0
V
Rep #3
Rep #4
v_

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: 12jfLud to - L tee-k Start Date: � Z! G'Fi Start Time:
End Date: Z/ End Time: il��1
Concentration 36 Y
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
U
C)
Q
V
U
0_
d
Rep #2
0
��_
U
Rep #3
V
�%
�_
Q
C)
Rep #4
`�
�-
P`\
�%
C'
Concentration
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1I—
Rep #2_
Rep #3 Ci
Q
Rep #4 U
UV
—
Concentration
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
J_
o
Rep #2
r,
U
-.
'i
rr
V
0
Rep #3 �/
�
Rep #4
�!
"
0
1
�.--

®. _ ®®
ENVIRONMENTAL LABORATORIES
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility:
NPDES #: _NC
Date of Test: 2-
Initial weights taken on: 12 JI- CS by: fV_L
Final weights taken on: by: W __
Page: I of 2
Outfall: co 1
Organism: Pimephales promelas
CONIC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WT./ORG. (mg)
SURV %
C
0
A
rM, 3G
SCs. 0
'7
I
D- (,qo
T
0
B
qq9 .2b
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802. 01
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I D
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1 w
A
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0-03
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119(a.cc,
I.75
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G. 5175
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0. S9,3
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100
D
0 , (
8 1 0, (8
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0. (o5 1 I
I

r..:.WHRITEC14, IMC.
ENVIRONMENTAL LABORATORIES
0,, _.on c.` L's rer Tech--I.gy Anti ronr,ots, t,—
FISH LARVAL SURVIVALIGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Ile - `tom Cte
NPDES #: A)C pC3;3i f30
Date of Test: I ---l?fox
Initial weights taken on: 12--11- 0� by: !vim
Final weights taken on: 12-ift- 08 by:AkJ—_
Page: Z of 2
Outfall: 00 /
Organism: Pimephales promelas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WT.IORG. (mg)
SURV %
3Co /
A
to
B
'�,0
0.53
G.y5
IU
0-Lq5
fC170
io.er✓,i�.5�1
f�.5`�
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0.�5`1
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D
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B
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4
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o
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6 1 83
s.ri4
o . Sq4

MERITECH, INC. Meritech Sample ID #:
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txmrtech(a-)be llsouth. net Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: —51 r
Contact Person: /- G&6 eon z PUO7'l >
Address: ,ae2��trn'�
City: �'��syz.�-��L
State: /VCR
County:
Sample Site:
Sample Type: ❑ Grab Composite
Sampling Time: Start Date: 0
End Date: / 0�
SAMPLE INFORMATION
PO#:
1 Y)V
NPDES#: NC QV 3/,?3
Phone:l012L J T
P�ipey#: r
Zip: 2TO2/
# of containers: Z
Start Time: - / 7 AM PM
End Time: TS AM PM CHRONIC TESTS SHOULD BE
AFTER 10:00 AM IF AT ALL POS:
—SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED AND COVERED WITH
Collector's Name: Print: ��� f,�T-7 Signature:
TOXICITY TEST INFORMATION
Test Required: Chronic (7 days) Test Organism: Ceriodaphnia dubia (water flea)
Pimephales prorhelas (fathead minnow)
El Acute (24-48 hours) [�
❑ Mysidopsis bahia (shrimp)
IWC.. % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
Date: Z q �� Time: d AM
Relinquished by:
Date: Time:
Received by: c� �J
Relinquished by:
Date: 1 Z — ` "d � Time: 1- �� � Am,
Received by: Date: Time: AM
Sample Temperature (°C):
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery "
SAMPLE RECEIVING (Laboratory Use Only)
c
Relinquished by:
Received b .
Date: ci cl f ci c Time: A
�7-, Sample Condition: ^ d
Sample Temperatures (°C): t U �',
PM
PM
PM
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. MeritechSample ID#:
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txmrtech(cbbellsouth.net Web Site: www.meritech-labs.com
\,=)vu�-9
CLIENT INFORMATION
Client: �7 f C%1ic�� PO#:
Contact Person: NPDES#: NC I/ 3j IfJ3,�
Address: &,;77
r_���J� /�� Phone:�f
City:-}�C�SIf7��I� Pipe#:
County: State: / V(f-- Zip: ZQ roZ�
SAMPLE INFORMATION
Sample Site:
Sample Type: ❑ Grab Composite # of containers:
Sampling Time: Start Date: Start Time: �` � AM PM
End Date:/j} /' End Time: /-.'AD PM —; CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
***SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED AND COVERED WITH ICE***
Collector's Name: Print: q
4t/ljzrz Signature
��,�l�
TOXICITY TEST INFORMATION
Test Required: j� Chronic (7 days) Test Organism: X Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours) ,N Pimephales pror►ielas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: .3 % Test Concentrations (if multiple dilutions):
Comments: TJ�12.��
SHIPPING INFORMATION
Relinquished by: c Pj�� Date: l Z�(O�D
Received by: Date: l y—1 o­Qf,
Relinquished by: Date: /7- —/�
Received by:
Date:
Sample Temperature ('C):
Time: AM PM
Time: AM PM
Time: 2­& AM
Time: AM PM
Method of Shipment: ❑ ups ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
(� SAMPLE RECEIVING Laboratory Use Onlv)
Relinquished by:
Received byDate:
Sample Temperatures ()C):
(O CJ'� AM
Sample Condition:
Time: D `�o
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. Mentech Sample ID #:
BioassaV Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txmrtech(d)bellsouth.net Web Site: www.meritech-labs.com
J /CLIENT INFORMATION
Client: �� `7 �� PO#: Contact Person: �/�7� ��j')S NPDES#: NC 0693-%Q-tOr9L �}
Address: ��� ��/l�rr /� CL Phone: ��— �?� �N36
City: _s!'�I s�G `� �Piipp�e/#: _
County:. State: Zip: lzs_a�5
SAMPLE INFORMATION
Sample Site:
Sample Type: ❑ Grab A Composite # of containers:
Sampling Time: Start Date: / i%/off Start Time: /gyp PM
End Date: l�14�99�End Time: Z17)00 AM PM —► CHRONIC TESTS SHOULD BETAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AND COVERED WITH ICE ***
Collector's Name: Print. in Signature:
TOXICITY TEST INFORMATION
Test Required: V Chronic (7 days) Test Organism: Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: % Test Concentrations (if multiple dilutions):
Comments: f / �� j � /�i14 L1
SHIPPING INFORMATION
Relinquished by: Date: / Time: 46 AM PM
Received by: 1` ' Date: `�- —�Z Time: 616— AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature (°C):
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery'*
((��
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: &C 3y-1,,,� / ! I
Received Date: I A [ G Time: Y AM �M j
Sample Temperatures C): Sample Condition: C
WHITE = Laboratory copy YELLOW = Client copy

Shapiro - Wilk's Test for Normality
---------------------------------------------------------------------------
D = 0.0285
W = 0.9514
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
---------------------------------------------------------------------------
Data PASS normality test (alpha = 0.01). Continue analysis.
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------
Calculated B1 statistic = 3.3032 (p-value = 0.6534)
Data PASS BI homogeneity test at 0.01 level. Continue analysis.
----------------------------------------------------------------------
Critical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)
ANOVA Table
---------------------------------------------------------------------------
SOURCE DF SS MS F
---------------------------------------------------------------------------
Between 5 0.0120 0.0024 1.5207
Within (Error) 18 0.0285 0.0016
---------------------------------------------------------------------------
Total 23 0.0406
---------------------------------------------------------------------------
(p-value = 0.2328)
Critical F = 4.2479 (alpha = 0.01, df = 5,18)
= 2.7729 (alpha = 0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Dunnett's Test
- TABLE 1 OF 2
Ho:Control<Treatment
----------------------------------------------------------------------------
TRANSFORMED MEAN CALCULATED IN
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
-------------------------
1
control
-----------
0.6348
------------------
0.6348
------ ---
2
9
0.5980
0.5980
1.3056
3
18
0.5892
0.5892
1.6165
4
36
0.6240
0.6240
0.3819
5
55
0.6568
0.6568
-0.7816
6
73
0.6205
0.6205
0.5063
----------------------------------------------------------------------------
Dunnett critical value = 2.4100
(1 Tailed, alpha = 0.05, df = 5,18)
SIG
T STAT 0.05
Dunnett's Test - TABLE 2 OF 2 Ho:Control<Treatment
----------------------------------------------------------------------------
NUM OF MIN SIG DIFF % OF DIFFERENCE
GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM
CONTROL
-------------------------
1
control
-------
4
----------------
-------
------------
2
9
4
0.0678
10.7
0.0368
3
18
4
0.0678
10.7
0.0455
4
36
4
0.0678
10.7
0.0107
5
55
4
0.0678
10.7
-0.0220
6
----------------------------------------------------------------------------
73
4
0.0678
10.7
0.0143

Title:
statesville - 4th
creek survival
File:
statessurviv
Transform:
NO TRANSFORMATION
----------------------------------------------------------------------------
Shapiro
- Wilk's Test
for Normality
D =
0.0175
W =
0.7713
Critical W = 0.8840
(alpha = 0.01
, N = 24)
W = 0.9160
(alpha = 0.05
, N = 24)
----------------------------------------------------------------------------
Data FAIL normality test
(alpha = 0.01).
Try another transformation.
Warning
- The first three
homogeneity
tests are sensitive to non -normality
and should not be performed
with this data as is.

Title: statesville - 4th creek survival
File: statessurviv Transform: NO TRANSFORMATION
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.

Title:
statesville - 4th
creek survival
File:
statessurviv
Transform:
NO
TRANSFORMATION
Steel's Many -One
Rank Test
- Ho:
Control<Treatment
----------------------------------------------------------------------------
MEAN IN
RANK
GRIT.
SIG
GROUP
IDENTIFICATION
ORIGINAL UNITS
SUM
VALUE
DF 0.05
-------------------------
1
control
-----------
1.0000
-------
------
----- ---
2
9
1.0000
18.00
10.00
4.00
3
18
1.0000
18.00
10.00
4.00
4
36
0.9750
16.00
10.00
4.00
5
55
0.9500
14.00
10.00
4.00
6
73
1.0000
18.00
10.00
4.00
----------------------------------------------------------------------------
Critical
values are 1 tailed
( k = 5 )

60 s I HIlic
W, lu -imal) mi

PV
MEMORANDUM
Division of Water Quality
- Aquatic Toxicology Unit
October 30, 2003
To: Kurt Trumbower
Environmental Sciences Branch, DWQ
Through: Matt Matthews �'
Supervisor, Aquatic Toxicology Unit
From: Sandy Mort
Quality Assurance Officer, Aquatic Toxicology Unit
Subject: Ambient toxicity test results
Fourth Creek at SR 2308
Iredell County
The aquatic toxicity test using grab samples of ambient waters collected from Fourth Creek at
SR 2308 has been completed. Ambient samples were collected on October 21 and 24, 2003 by
Kurt Trumbower for use in a chronic Ceriodaphnia dubia pass/fail toxicity test. The test using
these samples resulted in a pass. Toxicity test information follows.
Test Type
Test Concentrations
Test Result
Control Survival
Control Mean Reproduction
Test Treatment Survival
Treatment Mean Reproduction
First Sample pH
First Sample Conductivity
First Sample Total Residual Chlorine
Second Sample pH
Second Sample Conductivity
Second Sample Total Residual Chlorine
3-Brood Ceriodaphnia dubia pass/fail
100% sample
Pass
100%
22.4 neonates
100%
24.4 neonates
6.89 SU
151 micromhos/cm
<0.0052 mg/L
7.41 SU
146 micromhos/cm
<0.0052 mg/L
Test results for these samples indicate that the water quality of these ambient waters has not been
impacted. Please contact us if further effluent toxicity monitoring is desired. We may be
reached at [phone].
Basin: YAD06
cc:
John Lesley, DWQ-MRO
Environmental Sciences Branch Water Quality Section

PV
MEMORANDUM
Division of Water Quality
- Aquatic Toxicology Unit
October 30, 2003
To: Kurt Trumbower
Environmental Sciences Branch, DWQ
Through: Matt Matthews �'
Supervisor, Aquatic Toxicology Unit
From: Sandy Mort
Quality Assurance Officer, Aquatic Toxicology Unit
Subject: Ambient toxicity test results
Fourth Creek at SR 2308
Iredell County
The aquatic toxicity test using grab samples of ambient waters collected from Fourth Creek at
SR 2308 has been completed. Ambient samples were collected on October 21 and 24, 2003 by
Kurt Trumbower for use in a chronic Ceriodaphnia dubia pass/fail toxicity test. The test using
these samples resulted in a pass. Toxicity test information follows.
Test Type
Test Concentrations
Test Result
Control Survival
Control Mean Reproduction
Test Treatment Survival
Treatment Mean Reproduction
First Sample pH
First Sample Conductivity
First Sample Total Residual Chlorine
Second Sample pH
Second Sample Conductivity
Second Sample Total Residual Chlorine
3-Brood Ceriodaphnia dubia pass/fail
100% sample
Pass
100%
22.4 neonates
100%
24.4 neonates
6.89 SU
151 micromhos/cm
<0.0052 mg/L
7.41 SU
146 micromhos/cm
<0.0052 mg/L
Test results for these samples indicate that the water quality of these ambient waters has not been
impacted. Please contact us if further effluent toxicity monitoring is desired. We may be
reached at [phone].
Basin: YAD06
cc: Rex Gleason, DWQ-MRO
Environmental Sciences Branch water guauty mcrzon

PP-PPPP_
State of North Carolina
Department of Environment and Natural Resources
Division of Water Quality
59 Woodfin Place Asheville, North Carolina 28801
April 8, 2002
.loft aC75 1 c DODO C�a&� L,"11f
CERTIFIED MAIL
RETURN RECEIPT REQUESTED
Mr. L.F. Hudson, Jr.
City of Statesville -Fourth Creek
P.O. Box 1111
Statesville, North Carolina 28687
SUBJECT: NOTICE OF VIOLATION
Whole Effluent Toxicity (WET) Testing
Copper and Zinc Action Level Policy
NPDES Permit No. NC0031836
Statesville -Fourth Creek WWTP
Iredell County
Dear Mr. Hudson:
This is to inform you that a review of your toxicity self -monitoring report form for the month of
January 2002 indicates a violation of the toxicity limitation specified in your NPDES Permit. You should
undertake necessary actions to eliminate or reduce effluent toxicity to acceptable levels.
The Division's Copper and Zinc Action Level Policy is triggered by two or more toxicity limit
violations that occur during the toxicity testing calendar quarter specified by the toxicity testing special
condition of your NPDES Permit. You have previously received attachments addressing policy
implementation and a copy of the Division's Toxicity Reduction Guidance.
You should initiate follow-up toxicity testing upon a single WET test failure which occurs
during the toxicity testing calendar quarter.
Your facility is currently monitoring for copper and/or zinc and is exceeding the action level for
these parameters. The policy states that whenever a facility experiences two or more toxicity limit
violations during a toxicity testing calendar quarter, the NPDES Permit will be modified to include
numeric limits for copper and/or zinc UNLESS the pem- ittee provides one or more of the following:
1. Instream measurements of dissolved metal during low flow conditions that demonstrate
compliance with the Action Level standard
2. A revision of the prospective permit limit using improved inputs that in concert with existing
or additional monitoring data demonstrates compliance with the Action Level standard
3. Toxicity Identification Evaluation (TIE) results that definitively rule out copper and/or zinc
as causes of effluent toxicity
4. Demonstration by an alternative method approved by the Division and EPA that copper
and/or zinc is not the cause of toxicity
The Division has evaluated your copper and/or zinc monitoring data. The Division has also
developed a prospective NPDES permit limit based on your facility's instream waste concentration, the

2
copper and/or zinc action level criterion and a translator procedure. Based on this data your prospective
copper permit limit is 52 µg/L and your prospective zinc permit limit is 453 µg/L.
The permittee, upon experiencing two or more toxicity limit violations during a toxicity
testing calendar quarter must either provide DWQ with:
a) Written notification indicating acceptance of the prospective copper
and/or zinc permit limit. Notification is due within 30 days after the date of
the second WET Notice of Violation.
OR
b) Written notification indicating your choice of option(s) as noted on page
one of this correspondence. Notification is due within 30 days after the date
of the second WET Notice of Violation. DWQ approval of options 1-3
(previous page) is not necessary as the Division expects work to rule out
copper and/or zinc as causative effluent toxicants to begin immediately upon
the second WET permit limit violation
Written notification (a or b above) shall be sent to:
North Carolina Division of Water Quality
Environmental Sciences Branch
Aquatic Toxicology Unit
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Please note that if you choose item b) above, you will be given nine months to submit
documentation that eliminates copper and/or zinc as causative effluent toxicants. Your final report
should be comprehensive and include all data used to support your conclusion(s). Should the data
indicate copper and/or zinc as the source of effluent toxicity or if the data are inconclusive as to copper
and/or zinc's role as a toxicity source, then the NPDES Permit will be reopened and the metal limits
specified above will be applied to the permit. The report is due nine months after the date of the second
WET Notice of Violation.
Three copies of the final report shall be submitted to:
North Carolina Division of Water Quality
Environmental Sciences Branch
Aquatic Toxicology Unit
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
You should consider submitting the above correspondence certified mail.
Failure to notify DWQ of your acceptance of a copper and/or zinc limit, failure to notify
DWQ of your option selection or failure to make acceptable demonstration to DWQ that copper
and/or zinc are not causative effluent toxicants within the stated time frames will result in
reopening of the NPDES Permit to include copper and/or zinc numeric limitations, as appropriate.
If the effluent is toxic, then we expect work to commence immediately with toxic effluent
samples. Failure to initiate work with toxic effluent samples during the study period is unacceptable and
will be considered a failure to make acceptable demonstration as cited above. We recommend a
minimum of three separate sampling events during the nine month period to definitively rule out copper
and/or zinc as causative effluent toxicants.

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Please note that your actions to notify DWQ of prospective limit acceptance or to notify DWQ
of plan option(s) and subsequent submission of a plan are contingent upon two or more toxicity NPDES
permit limit violations occurring during a toxicity testing calendar quarter.
Also note that the WET limit will remain in your NPDES permit regardless of whether metals
are the source of effluent toxicity. The data resulting from your actions to disprove copper and/or zinc as
sources of effluent toxicity will determine whether NPDES Permit limits will be assigned for these
parameters. You are responsible for initiating actions to address these issues.
You may consider entering into a Special Order by Consent (SOC) with DWQ. A SOC provides
regulatory relief for specific NPDES permit limit violations and, if signed, will contain a requirement to
conduct a Toxicity Reduction Evaluation. The SOC will contain a compliance schedule, stipulated
penalties for failing to meet milestone activity dates and may contain upfront penalties to settle past
permit limit violations.
Additional information regarding the Division's copper and/or zinc action level policy can be
found at the following web site - http://vvww.esb.enr.state.nc.us. Click on the "Aquatic
Toxicology" Unit and go to the prompt "DOWNLOADS" located at the bottom of the page. This web
site also contains EPA's "Toxicity Reduction Evaluation Guidance for Municipal Wastewater Treatment
Plants." Section 4 of this document contains pertinent information on Toxicity Identification
Evaluations.
If you have any questions concerning this correspondence, please contact me at [phone]
or Mr. Matt Matthews, Supervisor of the Aquatic Toxicology Unit at [phone].
Sincerely,
Rex Gleason, P.E.
Regional Water Quality Supervisor
cc: Mooresville Regional Office
Susan Wilson-NPDES Unit
Tom Poe -Pretreatment
Lydia Mayo-USEPA Region IV, Atlanta Federal Center, 61 Forsyth St., SW, Atlanta, GA 30303
Central Files
Aquatic Toxicology Unit Files